<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="https://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDN1</accession>
    <name>A0A1E3NDN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_22381</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015358.1">
        <property type="nucleotide sequence ID" value="XM_019162253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDN1"/>
    <dbReference type="GeneID" id="30178940"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000012">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003154">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF5">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="37"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="325" mass="35848" checksum="4D1B0B6D2EAF417F" modified="2017-01-18" version="1" fragment="single">LHLELPWVEKYRPQKLGDVVGNTETIQALRRIAQDGNVPHLILSGTPGIGKTTAVLCLARELLGGDGRLLKEAVLELNASDDRGIDVVRNMIKTFAQKKVTLPHNRHKMVILDEADSITASAQQALRRTMEIYSNSTRFVFCCNQSSKIIEPLQSRCSILRFNRLRDEDVLLVLKRILHHEHVHTYTDDGLGALIFTCDGDMRQAVNNLQSVYYGFGLVSSDNVFKLVDMPNPLVVRRMLTDALRGHVDAALAVLRDLCGKGYSALDIVNVCFRVCKTIGGDGVDGGRQLEVLRRVGECQMRVVEGVGSYLQLSGMVVGVAGVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDX6</accession>
    <name>A0A1E3NDX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rrp15p-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_37183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015443.1">
        <property type="nucleotide sequence ID" value="XM_019163065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDX6"/>
    <dbReference type="GeneID" id="30179752"/>
    <dbReference type="OrthoDB" id="20949at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012459">
        <property type="entry name" value="Rrp15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245:SF14">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07890">
        <property type="entry name" value="Rrp15p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25689" checksum="D080F3DB09093BDB" modified="2017-01-18" version="1">MSVQVSKKRQRENKDDKAATKSKAEPVSAPVEETKESESESGSEQDSGSGSESEEAEDEKEVEAEEDDDDIPTVQKKQKTSAKKEAESFSNAMNALLDTRLKAYDRRDPILARSKKELKKFEDVKLEQKAKRLLLAEKKKKLTANRNKDLLPKNDATARQVLQHEKQLKKTAQRGVIKLFNAILMTQTSTESDMSHETGLVGQSKKKELVNEISKEKFLDLVQQVGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDZ4</accession>
    <name>A0A1E3NDZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015463.1">
        <property type="nucleotide sequence ID" value="XM_019164814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDZ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDZ4"/>
    <dbReference type="GeneID" id="30181501"/>
    <dbReference type="OrthoDB" id="9973266at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23946" checksum="8874C1FB1B0A84C6" modified="2017-01-18" version="1">MSEEKIYISYNHIHQLCQEVAPRIMELDIDFIIAIGGGGFIPARILRTFLKQPGESSKRIFAIILSLYEDVQTRGDDAEEVGTGVKRIQWIDYMDSNIDLVGKKVLVIDEVDDSRTTIHYAINELIKDAETQAGGELKTDFYMFVLHNKLKEKRAELPACIAKAGHYIAARETPDCWVAYPWESDDIVHHQLMAEKQGYDIDIDRSSLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE79</accession>
    <name>A0A1E3NE79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-aminoadipate reductase</fullName>
            <ecNumber evidence="6">1.2.1.31</ecNumber>
            <ecNumber evidence="5">1.2.1.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">L-aminoadipate-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_18343</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(S)-2-amino-6-oxohexanoate + AMP + diphosphate + NADP(+) = L-2-aminoadipate + ATP + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.2.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-2-amino-6-oxohexanoate + NAD(+) + H2O = L-2-aminoadipate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12308"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(S)-2-amino-6-oxohexanoate + NADP(+) + H2O = L-2-aminoadipate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.31" evidence="6"/>
    <dbReference type="EC" id="1.2.1.95" evidence="5"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015554.1">
        <property type="nucleotide sequence ID" value="XM_019161956.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NE79"/>
    <dbReference type="GeneID" id="30178643"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00032"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004043">
        <property type="term" value="F:L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05235">
        <property type="entry name" value="SDR_e1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000046">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000787">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014397">
        <property type="entry name" value="Lys2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010080">
        <property type="entry name" value="Thioester_reductase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03443">
        <property type="entry name" value="alpha_am_amid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01746">
        <property type="entry name" value="Thioester-redct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF1">
        <property type="entry name" value="L-2-AMINOADIPATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001617">
        <property type="entry name" value="Alpha-AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="18">
        <location>
            <begin position="838"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ44441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1388" mass="154370" checksum="9713657B73ADBCF8" modified="2017-01-18" version="1">MLDFEFWTKTLDSPTVNIIPFDFQRPTDGSILQDTFEFVLPEGTDFFTALTVYALLSYRLTGDDDITVATNDEFAANEFVLRLSIDPKKTFAELYNQVQSTYEEFYSKIVPIEKIVDVLKTSKSLEKAPALFKSSFQYSGDADSLETTVPGSVRDMAFYFGKRGTLSVYFNSLLYKKSRVEYLAEQFTQVVKAVDHDKTQVISKISLITESQKSILPDPTIDLDWSNFRGAIQDIFSDNAAKFPERTCVIETASFLDPSAKDRHFTYKQIDEASNIVGNFLVKTGIKRGDIVMIYAYRGVDLMISVMGVLKAGATFSVIDPAYPPTRQTVYLKVANPQGIIGIKKAGTIDNLVEEYIRDELNVITRIPNIEVQDDGSLLGGAVEGVDILADYAQFKSQRTGVVVGPDSNPTLSFTSGSEGLPKGVLGRHFSLAYYFPWMAKTFHLSEKDRFTMLSGIAHDPIQRDMFTPLFLGATLLVPTQDDIGTPGKLAEWMGKHNATVTHLTPAMGQLLSAQATAEFKSLHHAFFVGDILTKRDCLRLQTLAENCSIVNMYGTTETQRAVSFFEIPSRAENPRFLETQKDIMPAGTGMLNVQLLVVNRNDSTQTCGIGEVGEIYVRAAGLAEGYRMNDELNKQKFVNNWYVPIDGFVAKDKEVSKNEPWREFWKGPRDRLYRTGDLGRYLPDGNCECCGRADDQVKIRGFRIELGEIDTHISQHPLIRENITLVRKDKNNEPLLVTFFVPKVVSELESFKTVSEEIEKITDPLVKGLVQFANLIKDLKLHLKKRLAAYSIPTLIIPMSKLPLNPNGKVDKPKLQLPSENQLNQVAVYSSNQQDDTEFTEMESKVKDLWISVLPNRPSSITPDDSFFDLGGHSILATRMIFELRKKLAIDVPLGTIFKHPTISSFANEVERLSQLEDGREVEESTPNDESSKIDYYKDAVSLSKSDIKTNYSTLEELDPTKPINVFVTGATGFLGTFIIRELLSREKLDINIHAHVRASSEHAGLERLIKAGKTYGVWDDSFKSKIHVVLGDLSKPQFGLADDKWNKLTETIDVVIHNGALVHWVYPYAKLRDANVISTINVLNLCGENKAKHFTFVSSTSTVDVPHYVHLGASLHSASEDKGGVLESDDLLAAKSGLGTGYGQSKWAAEYIIRRAGDAGLRGAIVRPGYVQGFSQTGASNTDDFLLRMLKGCVEVGSYPEITNTVNTVPVDHVARVVVASALHPPRAPTEKLSVVQVTGHPRIQFNKMLDALNSFGYSVKKSEYREWAKTLEKYVIDDAKDSALFPLLHFVLGDLPEDTKAPELDDRNAVEVLKTDKLWNSDSKDYSSGSGVTKDVLAAYVAYLIKIGFLPEPKGTATSKLPDVEVKEESLELIRAGAGARTSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEJ8</accession>
    <name>A0A1E3NEJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17989</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015678.1">
        <property type="nucleotide sequence ID" value="XM_019161838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEJ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NEJ8"/>
    <dbReference type="GeneID" id="30178525"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF128">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN RGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="6">
        <location>
            <begin position="16"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="479"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="312"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="181"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="347"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="75967" checksum="5398B7B77653136E" modified="2017-01-18" version="1">MSNTNAPSSAELLQDDRVKAILASDSSFDILSQRLKQSVKACEEFSSYVTRKCHYEQKYSKEVARTTNHCNHSIVTNNLIVQGSFIQSLQKVIQFDEKVNGVRDPYITALQTISNQLESLAHHFTTLRKQLKEKGNRAEKEVIDAINLAKKARNKYWNLCSELEKVRNSDKNQTKITLQGRKTNSQQEEDLKSKIKDAEDEYNKKANTSQRLKNVLIKTHRPNIANDFKNLIVELDYAMQIQLQKYSIYTESLVVGMGNQINPINGSDSMKKIATSVDVEKCLYYYLKGSTVKSNENLEPVDFTRHPIVGGILPKPKSTTAATLSNNSGKANNNTTTNKALATATGSTTNNINSKPATHMNSNPTFNTSVNSNLSPPFTSPTKNTQTSQSEFRSPASSNSDAIIPPIPSLENKAPPAYSSLDPGRHSPLLNGPRPVESEINANSSTSSFNDNSINFNNGDNPVESSPASTSEEGKLFGLPLEVLDINEEAVPMFVTKCITMIETYGSNSEGVYRLSPNKANLEELQVALDKNPENLSLLEPQDTTNVSSEYVYLIASLMKRFFAMLPEPLLTNEQRDVYLQAAQLSDKTERQTKMHQLVFELPDSNYFTLRDLLGHFKRLIQMPRVRMDSKNISIVWANNLLGGDFNSKEELDVQQRVVEDLIAFAPFIFTYEEEGTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEL4</accession>
    <name>A0A1E3NEL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015675.1">
        <property type="nucleotide sequence ID" value="XM_019160033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEL4"/>
    <dbReference type="GeneID" id="30176720"/>
    <dbReference type="OrthoDB" id="3995457at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="425" mass="49361" checksum="4E539B17B7017E24" modified="2017-01-18" version="1">MNSRVVFTRDFSGESDSEYQDKSLGTTTESRKLYTRIRITNADPTLKRNLSDEVQDKSDPVLSYMRGGLAKHEVTDNESFDNWGNIIIWVDILAPKNEDFVQYQGHIDGHLIRKVTEDGIAKGIGLGQMHVWIYGLLMDGKYDGSNYEFGLVKVDERLFWSIRILHLGLFKMLFKVELTENSTIDIDEFFVEDLERFYKESIEHRKKEEKWKLEKTRYMHQINLLNDKKEIGKLGNSVRLMKNRITNYFLPLVNSLLEDKRELVEKMDGKDDTFFTSTRKRKTDNLDNDMKGVKMEEEVQQGKKMNDDDEDLDWMQRERNKRMKLLNYTPKMIAETSETRPLENSHVSSTTDDSLVFNKRGRSFRPDTQQDTSEATANSGKKETSRDSSVILEEETQADEATDVSQEKNIDLTDTSGEVTEISDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF03</accession>
    <name>A0A1E3NF03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerophosphocholine acyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_18109</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015823.1">
        <property type="nucleotide sequence ID" value="XM_019161880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF03"/>
    <dbReference type="GeneID" id="30178567"/>
    <dbReference type="OrthoDB" id="406287at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021261">
        <property type="entry name" value="GPCAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201">
        <property type="entry name" value="OS01G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="204" mass="24133" checksum="F0A0759E29822A55" modified="2017-01-18" version="1">MYVITHQLPYDYKLTRFSGSIKLQRWDIMYGIFNTSILYFAWQLCYHYFITIRKADKIKKGKMTSFEYLRKAFAERPIGKFVNSLPEPFPVVAFTIIQYGFQLITMSVCPFLYSYKHVCSLFVSFIFLCATYNGATYYVDFYGKKFQREVMKLQEEIELLHSASEHDHEKRGELQKDKKVSDDGISCTDMQRRRIDSISVSEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF45</accession>
    <name>A0A1E3NF45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U2 small nuclear ribonucleoprotein A'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_18135</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015857.1">
        <property type="nucleotide sequence ID" value="XM_019161892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF45"/>
    <dbReference type="STRING" id="763406.A0A1E3NF45"/>
    <dbReference type="GeneID" id="30178579"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="240" mass="27577" checksum="33C2ABCB67453EC4" modified="2017-01-18" version="1">MKLTQAVLEEAASFISPTEARVLSLRNLNLTSLDAIHVLQSSDIHSIIDITKNSISYLPEFPVLLRLETLILSNNHIRSVTGLAKLVNLQVLSITYNEIVHLTDLEELKELPSLRSLYLTGNPVVKNKDYRLWCIWRFPNLQIIDFKRVKNVERKQASSAFDNNSDKVKAILSVKPIPIFATKTLNREEQYVMDNVDTEEKEDTKPMTEDDRDRLEKELEQADSMEEIQRIEAILMRGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF67</accession>
    <name>A0A1E3NF67_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015331.1">
        <property type="nucleotide sequence ID" value="XM_019160109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF67"/>
    <dbReference type="GeneID" id="30176796"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="353" mass="36081" checksum="9FCD591D6C8B7258" modified="2017-01-18" version="1">MTTVSTGSSFSAPYRNSTTVTPLTTISSTPLSSLPSFQTSTTSTSEVTISSESSVLSSMKTDSSFTTPETTSTVGTTTTSTTSTYEVTIPSESSVLSSMKTDSSYTTPETTSTVGSTTTSTTSTYEVTIPSESSTLSSMGTPSSFTTQEITSSIGTTTAPRTVTSEVTVETITTGYTTTTTVFNKTFTSGSVTVTTILTETTVVPCSSSTYTTVVPLTTTPASPTSSSFPSAQTPTTGTFEVTVPSESSTLSSLKTEFSFTTQETIGTAGTTSVTAPTSAGPTFEASTTSGASATTLNTYTRNPGTSESATGTSSGTGQTNSVTVSYFNSSPKSSVGNGWLSLFAVLSTIFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFA0</accession>
    <name>A0A1E3NFA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_177298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015912.1">
        <property type="nucleotide sequence ID" value="XM_019161713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFA0"/>
    <dbReference type="GeneID" id="30178400"/>
    <dbReference type="OrthoDB" id="5555533at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037652">
        <property type="entry name" value="Mim2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19117">
        <property type="entry name" value="Mim2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="488" mass="56187" checksum="85F8825843867AE4" modified="2017-01-18" version="1">MEGHLNEEILSKLDKLKKDAQVEEDEEEEIDDFWEKKEHERKMKHMKEVEEQKSGKGKEEKHAKANAEQESQTEQIGKTAGEADTFDIATEQENLSQKEVKENRTAIETEQRKAVEEADREKTAEKQRLADEKKTAEEKNKAEEIERKRIEEAKIKAQEAERKRAVEEREKAEKAEQARMEEERKRVEEEKRIAEENKRKAAEAANAEKQKIAEEKKKQEEAEKKRVAEEAERKRMAEEEEKKKELEAKQKRLAEDAEKLRVEEEKRRIALEVAQKKLAQEAETKRLAAVEEEKKRLAGVAEKKHLEAQQKRLAEEAEKNMLAVEAEQKAIEEQQEKLAAKAEKKRKEAESAKAEASRLVKEAEMKKQAKLKLAELAKQKFEAQKLKERLKAGLSADSATGSCNDEPGAGEEDEDEDEDEDDDDDSVYTYSTAVTESNHDEEWDNLVLQLQVVIAIVLIPLAGKFLGRRFAHKVGSFVSGWFFSRNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFE4</accession>
    <name>A0A1E3NFE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015963.1">
        <property type="nucleotide sequence ID" value="XM_019161580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFE4"/>
    <dbReference type="GeneID" id="30178267"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="72" mass="8276" checksum="100DD863F878CBA8" modified="2017-01-18" version="1">MALAKLKSSSFIGVIFRNYKLNSSYKMKLHCSRDAEVQAFFVGTSRELHTSEDTELCTIILSPDKLRLDYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFF8</accession>
    <name>A0A1E3NFF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="3 11">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_17411</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="3 11"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015998.1">
        <property type="nucleotide sequence ID" value="XM_019161592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFF8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFF8"/>
    <dbReference type="GeneID" id="30178279"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000050">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000044">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="252"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="590" mass="67596" checksum="5C0A7895CEF2410D" modified="2017-01-18" version="1">MSDSTEKQVEQVAEGLQKTYLDEPSGEYVSKSELKKRQKLRALELKKAEKAKNAAATAPKISKQIDELANVNPAQYYEIRSAQINGLRQPHTENNPYPHKFHVSSEFEPFIKEYSHLKKGETLPDVKVTIAGRIMVKREAGQKLKFYNLQNNGVNLQVMAQAQDAEGDYAEMHKLLRRGDIIGVEGYPGRTNPSKGGEGELSVFATKVKLLTPCLHMLPTEHYGFKDQEARYRKRYLDLIMNKRSREIFLARSKIIQYIRRFLDTRDFIEVETPILNVIAGGATAKPFLTHHNDLDMEMFMRIAPELYLKELVVGGLERVYEIGRQFRNEGIDMTHNPEFTTCEFYQAYADVYDLMDMTELLFSEMVKDITGDYKVKYQPEGPEGETLTLDFSRPWKRVNMIEELERIFDVKFPSADTFHTQEFNVFLKNILVKHKLDCPPPLTNARMLDKLVGEIEDASINPTFIFGHPQIMSPLAKYDRKIPGLCERFEVFVATKEIANAYTELNDPFDQRARFEEQANQKAQGDDEAQLIDETFCNALEYGLPPTAGWGCGIDRLAMFLTNSNTIREVLLFPTLKPDALVKGAEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFH4</accession>
    <name>A0A1E3NFH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015993.1">
        <property type="nucleotide sequence ID" value="XM_019164553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NFH4"/>
    <dbReference type="GeneID" id="30181240"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="6">
        <location>
            <begin position="246"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="443" mass="51262" checksum="43B9516BDFE29F6A" modified="2017-01-18" version="1">MDLRSIYLLPVHWYLLWAVLVPVCLTVIPPRPYRAYVSTALLYAVLVKAQFFSPIDTSGGDASVALILQGVLLNLFFYTFNLFFLVEYPELTDYRPRYETREHVRALRPCTWQKFSWCVQRSVLVSLTGNGWNWQMSSVEHHRRRLHVSTWLKHFLVNKLLVGYTLYDVFFHIYLSTAYIRTQGWGPGHTRDLVLFASDSTLPVWKQLILAIGSAYCIHFGIDTLYTLTMIVHVFVLRTASIDEFPPLFGSFQGQYTVRSLWGNVWHKLMYQLAIPQSKFLAGCDYKATHRNDRPRYGTETWRKYLMFFLVFTFSGIFHAAGTLNMPWSLGAGYNTNSPFSGIHSGTLSGVLSRCFYSFIFFPAQFVLIILETLVQTLWNKCLRIRLPPAVTAAIGLTWICLSEVYLLQFYIDELTKSGFNVAELVVPFTPVHYIFSHFNIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFH9</accession>
    <name>A0A1E3NFH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptide transporter PTR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_17407</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015992.1">
        <property type="nucleotide sequence ID" value="XM_019161591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFH9"/>
    <dbReference type="GeneID" id="30178278"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="527"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="591"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="73920" checksum="D815714F04413D58" modified="2017-01-18" version="1">MFDKLKEKSFFSSGSVRSLNSSDNTHNIDDYVDSDRVMESSPEYVNNEKKSLSDNSFTKEGIASGEFEIFSDHEYMAEYADEYNPSGLAVATEEDKHKFRNVTTRPDFAAMMILICEFAERGSYYGVQGILTNFIMRPLPEGSRTGRVMPGHIGNAGALGLGLKDANALVMLLQFLAYVMPLVGGYVADDRMGRFKTILIGVWVGIISHILFIIAALPPVIAKGQAAMAPTVLGILSLAVCTGFIKPNLLPLMLDQYPYRTNVLKKLPTGEVVYVDRDHSMETLTMIFYWSINIGAFLQIATSYSAKDVGYWLAFLCPGIMYCIVVIAMLFVRPHLKPEVPQGSILKKLFQVLKVCLKGNWIKRLKNKEFWSYAYPTNMNERGEFFMNTKKQIPIDWTEQEVRDYKSTLTQCSMFSFWVIFNLSDTGLGSTLTAQAGSMSSQGIPNDFFNNFNQITIVAAIPILDYAIYPLLKKMKINFKPVHKIFVGFMLGACASMVSAILQWRIYQTSPCGYYATTCDTPSPLTAWYEIINYGLCALGECFCYTTAYELAYTRAPDNAKGLVMALFLFNSAISSAISEGVSGALIDPNLIWPFTGIAAAGGFCAIVFLVVYWNLDKVMEKEAEERELARQEQILMPVTSNAQSLDPEAEFEMRSAVQSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFQ4</accession>
    <name>A0A1E3NFQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_36243</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016029.1">
        <property type="nucleotide sequence ID" value="XM_019162994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFQ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NFQ4"/>
    <dbReference type="GeneID" id="30179681"/>
    <dbReference type="OrthoDB" id="1688503at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_uL24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13469" checksum="1A1C47B78ADEBC81" modified="2017-01-18" version="1" fragment="single">DVSSSRSKSRKAYFNAPSSARRVMMSAPLSKELREQYGVRSMPVRKEDQVVVVRGSKKGQEGTISSVYRLKFAIQLEKLTKEKSNGASVPLNIHPSKVVITKLHLDKDRKALIARKGGKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFV4</accession>
    <name>A0A1E3NFV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_36007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016134.1">
        <property type="nucleotide sequence ID" value="XM_019162978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFV4"/>
    <dbReference type="STRING" id="763406.A0A1E3NFV4"/>
    <dbReference type="GeneID" id="30179665"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF128">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,5-BISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="4">
        <location>
            <begin position="143"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="2">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="310"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="376"/>
            <end position="382"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="577" mass="67875" checksum="F600D1B3207D86F3" modified="2017-01-18" version="1">MEYLKITKVDDVVMYRRGQRYSGTLHLTTHHIIFTIVPDTTSESKSRPSELWFCYPMIERVDFNRGSALLYEADTKSRQRKLCRGASIRIQFRDFNYISFDFRNILKGNDVFESMMRLTCIDTIDKLYAFIYQPIRIETVFNGWEDYDVRREFERQGLTFDASDGDVGVEKTVGCSQWRITSLNKKYELCASYPRELLVPKNISDTMLGHTVKFRSKNRFPALSYYYKKNGCSIVRCSQPLVGIKQNRSIQDENLLQEVFRTNDLVKGKNLIVDARPLTNAMAQVALGAGTEIMDNYGDNNRKVFLNIENIHVMRDSLNKVKLLLRNSDVNGCNVPLNQEALNKTGWLEHISKLLRATELLVKHLVYEGVHLVVHCSDGWDRTSQVCSLVELCVDPYYRTMEGFIVLVEKEWVSFGHRFNERCGHMQRESKFYNNTEEGNFQKIRNLNQRFKHQQNMKFESPIFQQFLDCVYQVLLQHPTKFQFNERFLRRLVYHLYSCQYGTFLIDRDADKQHLRLPDRTRSVWDYFNSRRPEFTNPDYAPPALPSADYEADVLDIDYGAVVWWSEVFCRDMNRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFX8</accession>
    <name>A0A1E3NFX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_60580</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015581.1">
        <property type="nucleotide sequence ID" value="XM_019163446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFX8"/>
    <dbReference type="GeneID" id="30180133"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="7">
        <location>
            <begin position="499"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="779"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="783"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="799" mass="90213" checksum="88D1D4EE4E6CDF44" modified="2017-01-18" version="1">MWHQRASPSPSTTFYRSSGEYTSSSSNEYSSSNTAFDSDTEKNGLHSPEGYSLGFTDNYDRHSGVGKRWRSPRLKRTVNRGRSRLKRLLCLDALQQGACYKFLLLLKLRLHCYLNKKVLCVLIITSALVVSLLQMYSPLAYYKPRSKWSYLVENRHDFVSDSKVLDLLEYLSENKRLHEKYLLRRDYKDRRITPARYLTHLRTHINETGGTLSESLRYGFSWQDWVDFDERLLPNMDFLLTHNGLPISGCSQFESEIGFPRDNHFKRDFLPNCTDLTPAEIAALANPNYPHFRATAPLDNLGLPIEARIVHGATFLYHHQPPPQRLIFVDGVSNTDLVVPVVHKEPAPPPKDPRSLEPVSELMAQVVAGLPPAESDWFNRAVAVDVARVNRNGGSAPKLALTAADFANPENLTQIQAALPGFGEAGTLDAVLHDSIAGQLAAHPGADYPKYFHEPRLADSRIGGSHYDWRFFNIKEVDNEYKRLSTLSRLMRAWLRFTNNERLPTWLAHGTLLGYSFNGYMLPWDFDHDVQLSAAAMWRLARDYNNTLVVDCTAGDGRAAAGQGHYLLDVSSNFFDRRRGNANGNNAIDARFIDVHTGMYIDITLVSTVADKHAALRRDVPGYDKVLRRELFRLLAQQRLALDGLADDDSPGGTLLACRNHHFYRLAELLTLERRRFEGEQAWVPAAWADVLDREFPRRHTAWRHEGYTWRSELALWVADRRCGRAALDRGGDSCAGDRFVAAMRAMLQFGEQQEQEGAGVGPVLPDWEAVAAADLEAAEIGSSRRGETAGEGRREAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG62</accession>
    <name>A0A1E3NG62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016248.1">
        <property type="nucleotide sequence ID" value="XM_019161674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG62"/>
    <dbReference type="STRING" id="763406.A0A1E3NG62"/>
    <dbReference type="GeneID" id="30178361"/>
    <dbReference type="OrthoDB" id="8059989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="197"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="808"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="925"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="925"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="984" mass="110051" checksum="C700971B36A6435D" modified="2017-01-18" version="1">MNTSKRSSVNGEDIQVDGSSNAPTRLFKPSQPSAHLSNISQLTMNDTTISSCDNDLLLEPLSFDDNPNMSGYDLSNLSANADNLRNLNALRTRDNLTEDNDQVHDYLNIVNIENNQLTLDDFNLDLGLDLDLDLDLDLDVDSTLSYSPPTHLDTKCSVPNSAISDTNTNTNSNLDTPPSKILHNSDFSVDDLKRSFTRLKQTSELINTEKVYICSLKILEKVYLNNFMSDTSTPIYFDVFKKCVTQLLKSHQSLYDGLSEIYEDWYQDSVNLFDTEKAHIDSNAQSPNFDKFNYVSSEKEYLEAIVNLIANSSIDVQTYSTYCSLFQRVLNFSSSKGIESYKRDSMIILNDYFVDHKNLEADLFIDQHLDTRFISVVQMPTNRMVRYKLILQSLLKHIELDEKNSIQLNYYEKTMERINLKIDEINSYVGNEDMQLQKIQQFRLFINKSNNSVLPNKLFLENLENLHLASSFGVVYAIQSKHLILNHEYMCGFLFKSHLILAKCSSVTSNSIDIKFIIPLMSIFEDFENTSSHFVTTYPDTIILKFEDKFSIYEVCLIFPDEKEKLLWQGQLKVNLSNLVTLNSSVNSSAYRKSDFQKNEFLFSDVQRNILSIDLNPEIDKFNVTSYIPNTMNSTIKSEKNNVIYNHDFFQFEVDHFVSELNSPKKILRSDSVTSLSSNNRTESYENDNSSIKTKTSTINAPTHSSPGGKSQLVRIALQERVFAQTCIKTVWSPQFQIYTLNTSISRSLSNIFFHSKISLLSMNNTPTTPASNTFVPPSPSFANSGGSLGSPIKLRNVKSMRSVREVSRKPSIAARGNSLAYSPQCGKSFTPEPIRNEQNKIETGLGHRSHSVRHSNSLRSLSSFLGASSSRSMASSSTINQKSPSNLRGSLKSISALQSAQAQAQAQAQAQAQAQAQAQAQAQASASASASASTSLASTSASASASATAQASATAPLPSAKLGNSVSKFWRSFKHTSAKKPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGB1</accession>
    <name>A0A1E3NGB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_35916</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016277.1">
        <property type="nucleotide sequence ID" value="XM_019162974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGB1"/>
    <dbReference type="GeneID" id="30179661"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900430">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000003">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="20"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="443"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="473" mass="51349" checksum="1ABDDB03D9F1E4EF" modified="2017-01-18" version="1">MSNDVWTTNLSAQYDGASMVWVMVSGALVWIMIPGVGLLYSGMSRKKHALSLLWASMMAICLVSFQWYFWGYSLAFGTAHSKFLGSLEHFCLMGVLGENGGSAGLPDIVFCFFQGMFAMITGCIMLGGACERARLGPMMVFLFIWMTIVYCPVAMWTWNTDGWLNVLGAYDFAGGGPVHQASGVGALAYALWCGKRRDPLTQKGIPKYRPHSVTSVVLGTVFLWFGWFGFNGGSSGDASLRGFYANVNTNLAAACGGLTWMFLDWFRCGHKWTTIGMCSGAVAGLVGITPAAGFVPVYFAVPIGCITAVCCNYGVDLKDILHIDDGMDVFAIHGIGGFIGSMLTGLFAADYVAASDFGVSINGGWINHYYRQLGLQLAACCSIDLWSFVVTSIILNVMNRIPFLKIRLSEEEEDLGTDATQIGEFTYQESNMFIPEPVRSRVVPGDANNADVNAVPLPEKSSTDEEKHDTDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGF5</accession>
    <name>A0A1E3NGF5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_167567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016305.1">
        <property type="nucleotide sequence ID" value="XM_019161332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGF5"/>
    <dbReference type="GeneID" id="30178019"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="139" mass="15227" checksum="C8DF8D691E51139E" modified="2017-01-18" version="1">MRKPRSAVLRKAPRLIYGDRRQLSSSPRGSPREQPTRCTLVGTALCVATGSHDLPRRACRYEQRLSPLLAFRAAAKPEQNQHNVAFPSPAHRVAAAAAATLGHAALEGEASPCPQGWKIIPQLCERGAALYHRRCLNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGL6</accession>
    <name>A0A1E3NGL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016339.1">
        <property type="nucleotide sequence ID" value="XM_019164642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGL6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGL6"/>
    <dbReference type="GeneID" id="30181329"/>
    <dbReference type="OrthoDB" id="406156at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="2">
        <location>
            <begin position="213"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="399" mass="46126" checksum="4CBD138864128000" modified="2017-01-18" version="1">MTSHQDETVQQIRKQYNFRPFVDAPASNEKVDLLDLTVIDLSKFDSGPDARKELATSLEKALTEYGFFRVVNHGFSPEFLDHMKSVAQSTFETTDEVKSKFFAGETKIEEDKDLELGVIRGTGFKPRGYWKYSNDTPDNVEFYNFRHFLHPEIFHNRIEYPEFVQYHLDDIRKYFSELHTEVQRKVLTLMDIILEIPEGTLYNNFFPAIENDTLNSGTGFGRFLLYHPVDQEYNKKTSSTWMRGHTDAGALTFILSQSILSLQIRSYADNQWKYVSHVPNSIIVNIGDTFKFLTDGYFKSSIHRVHTAPEDQKNYTRNTVIYFASPKLDIYMDSGSLASPKLKRLGYKLDDGLERITVRQWDEAKGKFFNKTSANRKTNLTLYGRESVGSLIGENPIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHB3</accession>
    <name>A0A1E3NHB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphatidylethanolamine-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_34840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016622.1">
        <property type="nucleotide sequence ID" value="XM_019162909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHB3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHB3"/>
    <dbReference type="GeneID" id="30179596"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF148">
        <property type="entry name" value="CARBOXYPEPTIDASE Y INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24955" checksum="EEEFF12960F68930" modified="2017-01-18" version="1">MGLVTISDSIKDALYKSEVIPTVIHDDHFTPKGFLMIQYKDSDKEVTMGNTLKVADTQEKPVVHFTLNLSDDHKKSPIRPDDRFTLVLTDPDAPTKGDDKWSEFCHYVVRDIKLNDFTLADTAPAPTTADGKNDIDSFGDKLTTVDLQGDALVPYMGPGPPPKTGLHRYVFLLYKQQRGKRPDAPADRPCWGTGVPGSGATEYAHKHGLELYAVNFFYAKNEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHD3</accession>
    <name>A0A1E3NHD3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_73767</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016081.1">
        <property type="nucleotide sequence ID" value="XM_019164580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHD3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHD3"/>
    <dbReference type="GeneID" id="30181267"/>
    <dbReference type="OrthoDB" id="2687452at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF54">
        <property type="entry name" value="PROTEIN ESCARGOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="238"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="378"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="622"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="719"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="801"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="415"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="631"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="807"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="831"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="922" mass="101936" checksum="C1A8C36FD35E4218" modified="2017-01-18" version="1">MVHTRLSTMPADGMGGEDAFAKDDNRAAKAASHGPTGSVGSVGSAGSAGSAGSAGQSGDAKSTRTSSSSQSMLDTAHNSISSASSAASACFKTAKAIQNAQNEHRSSRQTKEGVEAAAGGIAEEQHSLPGDFGSDAVKMNDHAVANTHTSDSSAEENGRSFNYPGIQNNPQDGDKRGVSEKTLFDNPSPFLHPAMKPMQQLQNSFQYPGFMPFTNAAGLPPNYVYPLLSSIYYNKYVPPQQPQQPQPQQQQQGNSPTELQQQMQRRQKSGANGTGSPFPMDSPLAREDGQKQSLENLFANGAAPQISEPYFQNNQVYGSAKTLQQYYQLQLQQLQQIQQLQQLQQFPSSLDPSISASLLDSINFKAFFLDQHHYQQQQLSGADSQRKTSLSHEQSDYDTNQQQQQQQPLQFPYSLHNQQQMQHGHLSENQTPQTSTPSTFGTPLPPQQPQLPNFQPQQFHQFQKLQHLQQLQQLQQLQQLQQLQQLQQLQQLKQAQMPQRAQSPSANNQLYDLPQQAQQNHPEVPLQSRSDSNVSAANFVPDSNAFGYAAINNSLYHPLSNNLLPDFSQTGGFNAGPLSVLSPALAATFAPSIPLSMIPNLMGPSIGSLPNDPKNMETNAALEKSGKMRPRSKDTSNDPDSPKPYRCAHCTWSFTRPSDLRRHLKSHNAPQYHCPFWNVSYATCPHRNNGSFNRLDVLKRHLKLVHYEPDNEMVFKLKRKNKKNEQDGEGNTDTDGKELAEVYDEETGDLVTRRNDGGYCLSCSVHFTTAKDFIGHVSECALNTPMQEWKYKKNGTIACVKKQGDNPDGELEERTDNEESDEEENFGRNRVNPNNKIKTSTIPPNLDPETSSLYCVDSIGKLAVLNSCEDNAGANEGGIEIDPKKRTYMVSVSDLDTKPEIMDEALKRKRGRPKKNYAVTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHF0</accession>
    <name>A0A1E3NHF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator HFI1/ADA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16852</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016662.1">
        <property type="nucleotide sequence ID" value="XM_019161365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NHF0"/>
    <dbReference type="GeneID" id="30178052"/>
    <dbReference type="OrthoDB" id="10264870at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22933">
        <property type="entry name" value="HFD_HFI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024738">
        <property type="entry name" value="Hfi1/Tada1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12767">
        <property type="entry name" value="SAGA-Tad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="53850" checksum="46C5205EFE99FFBC" modified="2017-01-18" version="1">MLAASHLSASTVGGAGASTTTGNAPPNGGSSNGSGKSNGFHDKHQQQHQIHHPTSKRVEISSFVSKFQKTLGNGWDAYQIAVSLFIVGKLSRAELLEQIAPVLSKKGTRHMHNRLLMSMLANCYRVEPLDGISTSLFGNTSKKRKIGSKSSKYEQLKKDVLSLSIRERVRLKGITKESGKKGMIQNVMVLSRQAMVPKVPIVTTNNTSSVSSSSPHPSATGAASSEANANKKGSNNLEMTLISVKDILDMINEPLCTETYELPERKRMRDIMLGLAREHGLLGGVSMKAVDVLYLGLQYHLKSIIGNVIDNVRAKRDADKKGVDTSKETTNEVDDFLEKKGATIDNCGVETGTKRKKLTITTEDLFDSFSLTPHMIQPYGTVDHLNDVMLKNDDDYDFVSRQTLQANTDWTNGEKVLEWDENNEHNLYVVPNKVTDVSYLLKSHEAIWHETTGHPDDPVPKINLALKDKDIGTPDELNWVINGLLSEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHP1</accession>
    <name>A0A1E3NHP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BCP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_24647</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016778.1">
        <property type="nucleotide sequence ID" value="XM_019162361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHP1"/>
    <dbReference type="GeneID" id="30179048"/>
    <dbReference type="OrthoDB" id="27543at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028983">
        <property type="entry name" value="BCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="286" mass="33216" checksum="61519D3F145ED8ED" modified="2017-01-18" version="1" fragment="single">DSDIDVSSTDDEELQEEEKIDEEDEIVNVDFDFYNLNPTVDFHATKNFLRQLFQDDSVFFPLSELTDLILEEGHVGTTIKADGMESDPFSIISVLNITNNLKSKAMQELTKYYIEKTSKNTQFNVILRQLFSPSSKHKVGLIFSQRLVNMPVETVPPLYTMLLEEMEKSEQREKEYDFDYFLIPSRVAKLVASMADKELDDEDETTARKRKKKDTALPAEYDYVHYEDEQLEQNALHYGYFEFTNKNVEADARRVFNDYGIEPQMNLILINKTSLAKAVAQMAKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHR7</accession>
    <name>A0A1E3NHR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_73178</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016777.1">
        <property type="nucleotide sequence ID" value="XM_019164406.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NHR7"/>
    <dbReference type="GeneID" id="30181093"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02736">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02583">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035698">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035697">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015700">
        <property type="entry name" value="RPC1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="253"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1465" mass="162900" checksum="B590FC772DAFC32C" modified="2017-01-18" version="1">MKHTVVDVSPKCITGLEFGALSDAEIIGQSEVEVFTRNLYDLEHGRKPVQHGALDTHMGVSANTGECQTCHGNLASCHGHFGHIKLALPVFHVGYFKATIQVLQTICKSCSAVLLEDAMKEKFLNDLKRPGLDNLRRMRILKKIQDQAKKQRRCLRCGSINGVVKKAAAGGGPAALKIIHDTYRWVGKKGAPEKEAWDEDFDRLFDDNPELEKFAKRCFDDLNPLKVLNLFKQIEPADCELLGMDPSRGGRPEMYLWRYLPAPPVCIRPSVIMADASNEDDLTVKLTEIVWTSSMIRAGIQKGISINSLMEQWDYLQLAVGVYINSDIATPSMMQNGGTKAAKPIRGFCQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPELVAKVLTYPERVTRYNKKKLQNLLINGPDKHPGANYLQKKDEELKRNLRYGDRHKLAKNLNVGDVVERHIEDGDIVLFNRQPSLHRLSILSHFAKIRPWRTFRLNECVCTPYNADFDGDEMNLHVPQTEEARAEAMNLMGVKNNLLTPKSGEPIIAATQDFITGSYLISKKDSFFDKATLTQMLTSMSDGITDFDLPPPAIFKPVFLWTGKQIFSLLIKPNKNSKVLINLDAKCKTFSPPAKGLPNEMSPNDGFVIVRNSQILAGVMDKSLLGDGKKHSVFYTILRDYGPAEAATAMNRMAKMCARFMGNRGFSIGISDVTPKDDLKEKKERLVEIAYAKCDELIELYQTGKLETQPGCDEEQTLEAKIGGLLSKVREEVGEVCIRELERSNAPLIMATCGSKGSTLNVSQMVAVVGQQIISGNRVPDGFQDRSLPHFLKNSKTPQSKGFVRNSFFSGLNPPEFLFHAISGREGLVDTAVKTAETGYMSRRLMKSLEDFSASYDDTVRNSSNGVVQFIYGGDGLDPLDMEGDGVPVNFNRTWTHSYFTTLNHSDEPLPPYKVREVVKSVLLPLKDALIRVDNVGKVVSKEDFDKIDYVDIYDAERGFYDSVSNYIEGKLTLLATHRVSRGLPSFDEEPEKKVIDDVKKEISVSQILKITEKMIRSFLDLCCTKYRRARVEPGTAVGAIGAHSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINASKVISTPIINAVLVNDRDERAARVVKGRVEKTLLEDVACYIEDVYKENQAFLQVKVDLKTIDKLQLELNIEQIADALITSKLKITRADVSIVGKSKIQVLVNDMVGDFKPTGRSSASKEIVMLAHKDPDERDRLEKNALFFKMQQLKRLLTKVVVKGFPEISRAVINVKDDDTKGLLIEGYGLKNVMATDGVIASKTKTNHILEIFQVLGIEAARSSIINEIDYTMGSHGMAVDHRHIQLLGDVMTFKGEVLGITRFGLAKMRDSVLQLASFEKTTDHLFDAAFYMKTDQVEGVSERIILGQTMGIGTGAFKLAAKDNGKAVNLKTKETLFDSLCKGVVPPSIQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHV2</accession>
    <name>A0A1E3NHV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_12186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016838.1">
        <property type="nucleotide sequence ID" value="XM_019159897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHV2"/>
    <dbReference type="GeneID" id="30176584"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="141" mass="16174" checksum="E845D2009B6C5235" modified="2017-01-18" version="1">MGAVGEPQHGRAGVPRAQHVDRRLLDQQQQRGQDACVRLLRRLQLLRRPLGVAVELVQPSVELGPAGVLVLRRHLVALQLDRPALPQHRGELRRLRAVRARPRRQPAGRPLSVEQHAQHERRHHDRRHRAQERLRGEPALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHW1</accession>
    <name>A0A1E3NHW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016835.1">
        <property type="nucleotide sequence ID" value="XM_019164420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHW1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHW1"/>
    <dbReference type="GeneID" id="30181107"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050031">
        <property type="term" value="F:L-pipecolate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF46">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="8"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="452" mass="50811" checksum="242E0AF20A3F45C0" modified="2017-01-18" version="1">MTLELTDKVVVIGAGIFGISTTLALAKRYPELKIQVVDRFEPPVPDGSSVDTTRCIRVDYTNEIYSELASESLAKIKKDPVISQFFHETGMSYTYDGKHDRWEDFYLAGKESAEKANQAHPEKVVSLDSTEEVFKSIHGKEGAVPSAIDIGRKTWWNKGYRNFSNGFIDAELAMKAYYERANSYPNVTFTFSEVEKLNYYPTTNKFKSVSLKNGEEIAADLAIIAAGAWSGKLVNLQNICKMSAVEVGWLKLTPEEQENWKDMSITTNLSTGINLFPPYNGEMKVLRRSPGYKNTVTVANPDPKANDSIEISYPRTKVTNPSDWIPKDAEIAIRENLKEILPSLYQRPFDRTRLCWLTQTPSANFLIDHHPHSENILLATGGSAHAWKFVPVIGDKIVDFLEGTLEQRLVELWSWREKLGDKADNGSAPRMVGEPQEISQVIRTSGNENLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NII2</accession>
    <name>A0A1E3NII2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_34482</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017053.1">
        <property type="nucleotide sequence ID" value="XM_019162890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NII2"/>
    <dbReference type="STRING" id="763406.A0A1E3NII2"/>
    <dbReference type="GeneID" id="30179577"/>
    <dbReference type="OrthoDB" id="9944568at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 63C, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="13"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="11">
        <location>
            <begin position="285"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="141"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="419" mass="46192" checksum="E08E9CD7B1175676" modified="2017-01-18" version="1">MALSKKEIKITTLLVIDTVFFLLEIIIGYTVNSLALIADSFHMLNDIISLIVALWAVNVAKNRSADAEYTYGWLRAEILGALINAVFLIALCFTILIEALQRMLSPPVITNAQLILTVGTCGLLSNIVGLFLFHEHGHSHDDETTHSHSHKHHKSLSGERSPLLSSDPAAEDQDLRQVLPSYVVGQLDENASSSTKLNASGSRKKAAHQKSLNMQGVFLHVLGDALGNVGVMLTALFIWKTDYSWKYYTDPFISLVITCIIFSSALPLCKRSSRILLQGTPLTVETSDVIQDILQIPSVLGVHDFHIWNLTEKLLIASLHVELNSSPESFLKIATEIKSCLHDYGIHSATIQPEFSAYYSQQHMLPHSFSSTQNLSHAILNNATQDEIPNPQKNLNRSSSEIHCLVDASANCTTGDCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIL4</accession>
    <name>A0A1E3NIL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_26332</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017090.1">
        <property type="nucleotide sequence ID" value="XM_019162443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIL4"/>
    <dbReference type="GeneID" id="30179130"/>
    <dbReference type="OrthoDB" id="18781at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000405">
        <property type="term" value="F:bubble DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17923">
        <property type="entry name" value="DEXHc_Hrq1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055227">
        <property type="entry name" value="HRQ1_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22982">
        <property type="entry name" value="WHD_HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="258"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="476"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1008" mass="114810" checksum="32B977C396E526B6" modified="2017-01-18" version="1" fragment="single">LETLHFKLVSYFTFLSTRKHIITSFDLFKEHLSKSLEKELTVEDVAKIKSIIPHNMIFEYVDKNQFILEEKHFTWKDGFKQKEADIFDIDKNDTESLNEQILVIEFIDSGNKSRHQNNFYKPMTPEEVKKLIIKRDNRFRKTFRNIVKTQMLGSADYINNLSLKYIPEKVKFINPMEQMNQTNIESNHEPIPKLIERFKKSSFYKNQISLTSTHRIPSKQAIYDPNFYDEINIHPDLQALLQSKGMENVYIHQSIGIKAVLSQQKHVISTTSTSSGKSLIYQLPILQSLLTDKNSTSLLLFPTKALSQDQHRALLSFIGDLPSINRDLIKTFDGDTNKTDRQSIRNSANVILTNPDMLHLTMLPNHKTWSRFLKNLKYVVIDELHTYKGSFGANVAYILRRLRRACSLFGNDSIQFISSSATLNEASLHLSKMIGLENSVDDIVWIDESLNGSPCGARNIIGWNPDLDHGGSMISDTAKLIVELLKLNIRTIVFCTIRKTVELVMKEVRFLLKDEPVLLGQIMSYRGGYSISDRRKIEHQMFNGGLTCIVATNALEVGIDIGGLDVVLMCGFPISLSGFEQEMGRAGRRGLDSMCLLVAGGDPVSQFYVNRIDELVEKKEWEDLCVDLKNLMIAEGQLQCAFKEKNVPNDELSNEWVNWWNCLPWEIYENLIRDKLKWEGDISRWTCDDSYLPYPPLMVSIRAIDEDSFAVVDTTGGNGNQEVIETIEASRTTFTLYEGGIFIHQGLPYIIKDFNVDEKYALVERVNVDWTTSQRDFTDVDPVLIERLRSLNETEIPIYFGNIVKTSIVFGFFKVDKRGKILDSVEVNNPPIKYKSKGVWIDFPQELNELIKNHKLNLAGAIHAAQHGIMNVLPRFVTIGSYDEVACECKAPEKEFASRETKRKRPARLILFDNKGGKAGNGLSSRVFECAEMVLQETLKIIEDCTCAFGCPLCCAGMQCTENSLVLSKDGCKIILRYLLKDKDPLRGIPFGPEGNMPEITIETVVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIN4</accession>
    <name>A0A1E3NIN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Carboxypeptidase</fullName>
            <ecNumber evidence="14">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_73467</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="14"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017102.1">
        <property type="nucleotide sequence ID" value="XM_019164486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIN4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIN4"/>
    <dbReference type="GeneID" id="30181173"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 14" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027319" description="Carboxypeptidase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="551"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="497"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="528"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="708" mass="80041" checksum="AEE863A3CE4F6D81" modified="2017-01-18" version="1">MLQICKTWICFWILFSSLTSAAYIPNLHVRATSNENNEFMIDSSLIPGFQESWNDILTMHAGHIPVTEDQSSLYFWSFQSVNETYSTGRTRPLIIWLNGGPGCSSMDGALMEVGPLRVHNKTSVIWNEGWLNVGDLVFIDQPLGTGFSSRNSGDNYDTNLQESSSHLFTFVEKYFEIFASDYDKYDSIILAGESYAGQYIPHLARLMKDSPKFTDKLEALLLGNAWLDPNLQALSYLPFAIDNGIVDPTLQEDSRKITVMLENQESCQNVRNTLVGKEKFEDEKCESIIMNLLRLYKKNKNKCINVYDIRKTDSYPACGNNWPEILPDVTSFLNLQGVQNALHVFSGDHDSSVWRECDNDVSSHYNPSDSVKGASLLNGLLMDGVKVNLFSGTSDLICNYLGSEMVMREYLSPYLQEHGYQILLKSLSEPHRLMKREVDKFKMDSKWLHDEAEVGSFWQRGNLTYIKVDNASHMVAYDVSGPSIGIVNLSLRDSSEGQQSEVKTYSSPEKKRQLELAEQQKEEEAKQQAESNPDSNNSDHSTTTTKSSKRLLALFLLIIVLAVLALYFFRVSTRKPSRYSALAGAHKPSTYGRYAYEWVSGTGGKGKGKNSKKKRVHWVDLEELDEDGSLSLPSKLDSNQTDNQKLVIGGDLENGRRMKSFEMEDLELDTDHEPFDSALQHPREGSFETEELEIQLQDLTKSHMGESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIP0</accession>
    <name>A0A1E3NIP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Respiratory growth induced protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73472</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the control of energetic metabolism and significantly contribute to cell fitness, especially under respiratory growth conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RGI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017107.1">
        <property type="nucleotide sequence ID" value="XM_019164487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIP0"/>
    <dbReference type="GeneID" id="30181174"/>
    <dbReference type="OrthoDB" id="4082176at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006112">
        <property type="term" value="P:energy reserve metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.40">
        <property type="entry name" value="Respiratory growth induced protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022554">
        <property type="entry name" value="RGI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038235">
        <property type="entry name" value="RGI1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10843">
        <property type="entry name" value="RGI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="190" mass="22305" checksum="00DECF620CECBDD1" modified="2017-01-18" version="1">MARKNLKPVHMEPVHIDHLEKAPAVRNSKEMDFDSLEDFEKYIMDESWDNEFDNLNIHVKYLPPFIVNQTKGNEDKIKPQMNSLNKKFRRHLQHHVKRHLLPDINRMAGIDYDFSKDGEEKVANQYGTSSFYKWHFTDETNHGFDESEYNSRNHWKVEVAIESNSNNPWIDVTYKSVAANDGEISPADIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIT7</accession>
    <name>A0A1E3NIT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Karyogamy protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_12492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017167.1">
        <property type="nucleotide sequence ID" value="XM_019159934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIT7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIT7"/>
    <dbReference type="GeneID" id="30176621"/>
    <dbReference type="OrthoDB" id="5559380at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051293">
        <property type="term" value="P:establishment of spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013889">
        <property type="entry name" value="Karyogamy_KAR9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37271">
        <property type="entry name" value="KARYOGAMY PROTEIN KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37271:SF1">
        <property type="entry name" value="KARYOGAMY PROTEIN KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08580">
        <property type="entry name" value="KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="655"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="691" mass="78385" checksum="0CEB493FE61E7DCF" modified="2017-01-18" version="1">MSSHAAASISFLIRKSFDLAKFNDLIDIEKQNAFIAEENLSEISLLSLQETSDQVGERIGYQINWIFEGRIELSSIFTSIDTLEISVNLLLSKSQDISNFKTESLLDTIGKICSSSLEVKRLISPYKKRLAIANHHNEIEKEVLGSVKNEILYCASKLDDLEKKKQMVDIRSQIQNWTLDDFIAKKKELASDLATNYNGLIVFAGLDAQIFDSYTSLHDTILPISQSLKLIPKALDDFYHNARIYYQNLILDNIDNYEQITALYEKYRQDLRTFKKYYITHRINLICEKIFEKVNTDHEQSNSVLEGVLDVLKPIDNYYGLSEDYSSKLKLIEDKFYNIPLEENVQTPPPKSMQCRSKRVFSNPLVDSLNMKPILSGNRIKKRPEVKIFQTPRNIDDDFHMKANTEILERIKAEIGDNSTASMQRIPDTPLTLYSPQSSDSSPDTMQTRNIFDSPDPFITPNSRSFQKSRLPISTPLTTPRITPTKNYMSVDEELKRKHDLVVKGNAHTPLLRFELSTIIKVDPKTKEPHFPISEATADMSKLSLSQSQIPFSCDSRACNESQAIETSKIPLPKRPESRLNMLRSVSRLQTDRSKNGRSSSLGFGRSDSRLENIASSISMKLGTDSPVLISEAKTRPSTVMAQREMKQYGRPPQLRQSTGSYSGQLRNMKTRGATSLGARTLASQSRVSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJC4</accession>
    <name>A0A1E3NJC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_126853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017357.1">
        <property type="nucleotide sequence ID" value="XM_019159959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJC4"/>
    <dbReference type="GeneID" id="30176646"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="83" mass="8944" checksum="B7E4914CC66F6DA7" modified="2017-01-18" version="1">MASEGQVSADRIYANTVVGEEPDKCNLQDVCDSVGKGSTLSNRWQTCKRRAGKGRDERGKLVAAVKSINPSGHFGQFGNTQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJJ3</accession>
    <name>A0A1E3NJJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017412.1">
        <property type="nucleotide sequence ID" value="XM_019164183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJJ3"/>
    <dbReference type="GeneID" id="30180870"/>
    <dbReference type="OrthoDB" id="5328412at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="486" mass="55790" checksum="342C4BF4519519A8" modified="2017-01-18" version="1">MRLPKSVLLRRPDVAEETNEAEPTTAKDFYDEGVSYEESGDRWFTSDLSKAIRFYHRAHENYKQALKLDPTMMHALYNLPRLEYEVYNKYTKDDAVVTSDLTNCADALNDTKAGGLFQDIVSLCRSFEGSIDILLQSGNESSMGWDFYFNSASCYFEYIETLCSDPTILQGLSENNDLVKAVQRCIYMFEKVLSYMNYSLENSIEDETVNPEAVSSVCIESYRMLSTVYETLYTMELISFMDNLTAEYLERVDFVSNKLVQDPIPKDIIITLKIAKLNERSSRQLDYDSLVSIWNSDPELNCTIEKQLTESSSVRSFLDKFETVEISIPDETKWLILTNLDNKYRGINTILCSQIDELAKSSNSENDLLSGKISLACSVFIERADIFLERSLLKVDEAAKNRTVLQNNCKNLLKNALIFSKKTGGIRESIIGKYTRKKRQREAAMRLCLIEGKTQEDWGKIIGENYWPLELQTLADIEAYKMLLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM1</accession>
    <name>A0A1E3NJM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_26374</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017424.1">
        <property type="nucleotide sequence ID" value="XM_019162445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM1"/>
    <dbReference type="GeneID" id="30179132"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="130"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="47887" checksum="88312707E16B1253" modified="2017-01-18" version="1" fragment="single">EVNGWLLNSEVSIPLKKNCIIAFGRLPHDENDDRVFRVEIDDPHISSTHCYVWMIQFDSDTPPVCYFQDVSTNNCFVNNVPVGKGNYRVLHNTDFVTLYDTIGFQYKVNQTGGQVTEVGIDRNCEIKNWVVLPEVLGFGTFGKVQIAYNKKEKGRYYAVKTIMVSQENERSKIEHEILSKIEHPNIIRIRESIIDEKQGAMKLFQELAVGGDLFSYLSQDSQVLKALPESEAVVALYQICNGLYFLHRRGIVHRDLKLDNILVMGAPIRYPHLAIGDFGIAKQHLPLGESQAYWMNTMVGTAEYAAPEINLSKRKKECWNNAEDFFIHKGCDGDGGDGASAAAARTNRYTEKVDSWSLGVVSHILLSGISPFYSDNLEQTISKSRRGAIYVDTSARWTAVSDTSKRFVKGCLEVDSRKRLSIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM5</accession>
    <name>A0A1E3NJM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial Rho GTPase</fullName>
            <ecNumber evidence="15">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_16847</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Mitochondrion outer membrane</location>
            <topology evidence="2 15">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016656.1">
        <property type="nucleotide sequence ID" value="XM_019161362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM5"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM5"/>
    <dbReference type="GeneID" id="30178049"/>
    <dbReference type="OrthoDB" id="10020961at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01892">
        <property type="entry name" value="Miro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000011">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000553">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021181">
        <property type="entry name" value="Miro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037488">
        <property type="entry name" value="Mt_Rho_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 15" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="612"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="184"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="429"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ45543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="643" mass="72586" checksum="74E8FBCCEA554050" modified="2017-01-18" version="1">MDTIRVVVCGDEGVGKSSLITSLVKEKFVPNIQHVIPPMNIPRDFSDSRYSVNSTILVDTSASNISSLQYEIRQSNVIWLVYSDHYTYERISLYWIPLFRSLGVNLPIIICNNKSDIDTSTESSTALNEEFIPLLKDFKEVEACIRCSAKENYNVNQAFYLCQRAVTHPLSPLYDYKDGCLKPLAVSALNRIFYLCDRDQDGYLSDDEFLLLQQKCFHKSIDITELQTIKNTLNGIIPGSASEKGITSQGFLLLNKFYVESGRHETIWGILRAFYYTDSLSIDDKVLYPKIDIPQHSSVELSPIGYKFLVDLFILFDKDNDGGLNDHELERLFYPTPGIPKSWKDNNFPRSVVCNEQGYVTLQGWLAQWSMTTFLDYKTTSAYLGYFGFETKTSNSKNTYSTSSALYITKPRKFKKRNGKVYRAAVNDRTVFNCFIVGASGCGKSSLLESFLGRPFSNAHSPTIQQNIAVNNVELIGGKQCYLIMEELGEVEHAILDNKEKLDSCDVLCFAYDSSDPESFQHLIEIKQMHNYLDNIPSVFVALKADLDRQQQRSDFLPEPYTKSLHLSPPLHISSNWLPSMNELMSHLVAAATNPRFATPGLDPEPTEPEPLLNSFTIGCGAIGLMAVASAWYLHGYLLGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJT3</accession>
    <name>A0A1E3NJT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CobW/HypB/UreG nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_34520</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016941.1">
        <property type="nucleotide sequence ID" value="XM_019162891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJT3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJT3"/>
    <dbReference type="GeneID" id="30179578"/>
    <dbReference type="OrthoDB" id="258627at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140827">
        <property type="term" value="F:zinc chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034224">
        <property type="term" value="P:cellular response to zinc ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="8">
        <location>
            <begin position="45"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="CobW C-terminal" evidence="9">
        <location>
            <begin position="293"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="44144" checksum="30A3F70F5C20FE7E" modified="2017-01-18" version="1">MTDDVELIPDLVEDGEEEITFHEIKKPAQDLSGAIIEEDKNKRVPVTILTGYLGSGKSTLLEQIAKRGDRKLAVILNEFGDSIDIEKSLTVKDKNSEVEEWLDLGNGCLCCTVKDNGVAAIERLVSKKKGFDHIILETTGLADPGPITTMFWLDDGLLSNVYIDGVVTVLDAKNIENCLDDKGNGHEAQLTVAHVQIALADVILLNKIDTVENDPELVKRVEGRVKSINSNVPIYETKFGDISLEKILDLHAYEARDVNELIKTTNTESNVHDHRMGTKTFNFRRLTDIQEFARFEKFLQNVLWDEGDEDAQHSTNGGEQMEIHRVKGLVYIGDTYRVIQGVRKTYDVIEGVQEMSEIKLSESRLVVIGKYIDQERISSTFTEFMGFPLASWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJU0</accession>
    <name>A0A1E3NJU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_20617</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017527.1">
        <property type="nucleotide sequence ID" value="XM_019162164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJU0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJU0"/>
    <dbReference type="GeneID" id="30178851"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903468">
        <property type="term" value="P:positive regulation of DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060903">
        <property type="term" value="P:positive regulation of meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905263">
        <property type="term" value="P:positive regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905342">
        <property type="term" value="P:positive regulation of protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904968">
        <property type="term" value="P:positive regulation of spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="17"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="380" mass="43466" checksum="58CBAD2C3F6A00E9" modified="2017-01-18" version="1" fragment="single">QELYSFTSKFPGLEESYKILQKIGEGTFSSVYLAKPVARNSLHKGINSTVALKRIYVTSSPQRIYNELKLLYTLRGHKNVAPLLDVLRHEDQVVAVLPYYEHSDFRDFYRDLPLFGIKSYMFDLLKALDFIHSKDIIHRDIKPTNFLYDPFSRRGVLVDFGLAEIDMEPITNKCPCSFGGYTSNMVRQLNVDYAGQFPKNGYLKDDQRPGRRANRAGTRGFRAPEVLFKCNNQSKKIDIWSAGIMLLTLLSRRFPFFNSTDDVEAIMEITNIFGITEMKECALFHGLEFDCNIPKIKDKISLEALIANAVMMDCKEGDTFGEDSPAWELLAAIDKKGVIQDNEIGREYAQAITVLKKSMVLNPYERLDAKDLLKLPFFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJW5</accession>
    <name>A0A1E3NJW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst complex component Sec3 PIP2-binding N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16321</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017547.1">
        <property type="nucleotide sequence ID" value="XM_019161165.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NJW5"/>
    <dbReference type="GeneID" id="30177852"/>
    <dbReference type="OrthoDB" id="27109at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13315">
        <property type="entry name" value="PH_Sec3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01313">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="6">
        <location>
            <begin position="83"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="196"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="342"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="222"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1382" mass="157994" checksum="5251F5962731CE64" modified="2017-01-18" version="1">MSHSMSPATNTQRTMGSFQPGHRPMNSMDLAKQYEIDMKKIHDSLFLEVDSTNSKVENYLAHVRIIEDAKSQSSRPPANSPQSNKKNRFLLLSVKTSGRMRLHKAKESSSGMIQIGRSWDFDELISLELDDQVPTGFVCQMGKKYYWEVHTPKERRVWCSTLVDNYIRYTQGNLPQLINCSVDYFHLENLYDSYHGGSSSVPSQNKTPKPQILSRTPVLSPKGNSPSTFANSQRSPSKSNSQDLTSLKKNLGNTNSANSSMAAAVRGASATTLPLSASSRSPDLDNRYKLEQERKMYEEQKQKEQLARKQQEEKRLIEARRQAEEQEHQKQLVIEQKRKQELEKRRQQELERRKQKELERRKREESEKAQQEEELERARKEQVEKDRLAEEQERSRQQEEVERAKQQEIRRQEEVLRMKQQKAQNLEEERREKERTNQVQLAEAATKVKKTGYENQQRLQNNGLSEIVKRGPPSGMLSNEPSQMSFEIGDEHRFQNINASVESDISLGIDNYIDGYTSNGEEEAQTAPLNLSKPRARSAVATQPIGKLGAPVITTTLDEISGVTNHENVSNLSIDSELNKLLTPVNHLESMENEIDKSFGEGNRHHARSRAFSRVSDSTPDNNDLLEILEEIGYDPIEDDSASLQKKLLKELDKLQYDKIETLIKVTGVSSALKQSIFTAFQNCDHIDPILALFGVQLSTFKEDVDFIEEQGQGLQVEATNEKLLMNELNEIVHSVEISDSKLQMLLGTKITLGYQNSELEKILNELYYALLKVSGSQEKLNVDHGISKMKALQEKKRSYENVRDTFVARFKKQAGRIFESVSLSLSSKLENVSPDNFDNFLNTIFFDKMTYLLTVEGLIAFVKSVSISDYYDIIDSYVAAMKPFFENLASVLMKKLEQNVSLLDVQQFSFSSDPANLIDKMYISSKSKKDFGSSRGGNMFVMNQSNNEKKLSGDNEIIKLINIYFSQATRVMGAEQEMMKKLFALSSSPDMSFENVVKLPLEKRCQEFVATSDFLNGTIESDREIGDYIFDIMRQLFDSTFGSLLKILLSVSKGNILLTPGILCLLKSSSQSLAPTSHEYLYGNFTKLETKVNSIWVKEIDQQSQEILSSKVRCQVMNFVKAYPVFFGRIHAIVDSLDLVNLVVFGTDQKVYGNYYMIWDIIMSALNKGVESMKFEVILQDSNSEEHDIDADASFEKHLTLWINYKWMFEESKSLLEFPKDLSRSIDDLRDRELRTFSECFGRRFKIGNIIRLVEDLENLLSNNGNPSTFSTYSVENIKSLMVAFKGDSFKEEISSLAKGLKSVLKGRCYFIGDNRESEFSIAIGEQIEKELYNNCMFALCQLYISTFSKLSSIVDQYYSNFEVPVDKYIINFNFKKNYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJX1</accession>
    <name>A0A1E3NJX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-lactate ferricytochrome C oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_73159</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016756.1">
        <property type="nucleotide sequence ID" value="XM_019164401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJX1"/>
    <dbReference type="GeneID" id="30181088"/>
    <dbReference type="OrthoDB" id="5332616at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2190:FF:000001">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000016">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000053">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051264">
        <property type="entry name" value="FAD-oxidored/transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716:SF1">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="78"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="521" mass="57681" checksum="9CAA3789158C3AC7" modified="2017-01-18" version="1">MLRSLLIGNSKNTTTVFKISPKRFSSQLTADKYAVKRGDFAKLSDEDLSHFHSILNPNQMITDTNDLIAFNQDWMKKYTGQSQLVLKPKTTEQVSKILAYCNERKLAVVPQGGNTGLVGGSVPVFDEIVISTASMNKIRSFDESSGIFKVDAGVILEAADTFLAEKGHIFPLDLGAKGSCFVGGNVATNAGGLRLLRYGSLHGSVLGLEVVLADGTVMDSMHSLRKDNTGYDLKQLFIGSEGSLGIITGVSILCPARPKSVNIAFLGLESYEMVQKCFQTARSDLCEILSAFEFMDHDSQLLAAKSLKTTHPLCQEDETGVDSVPEYPFYVLLETSGSTKEHDDEKLENFLEKVMGDETVVDGTVSQDETQLRTLWTWREGIAEASQQFGGVYKYDVSLPLDSMYSLVEAVQERLKKLNLSSTEDESKPVYEAVGYGHIGDGNLHLNVAVREYNKEVEKALEPFVYEFVSKHHGSISAEHGLGFQKKNYIGYSKSEVEIRMIKDIKHHYDPKGILNPYKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK43</accession>
    <name>A0A1E3NK43_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_11454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017569.1">
        <property type="nucleotide sequence ID" value="XM_019159798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK43"/>
    <dbReference type="STRING" id="763406.A0A1E3NK43"/>
    <dbReference type="GeneID" id="30176485"/>
    <dbReference type="OrthoDB" id="4068492at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053278">
        <property type="entry name" value="Pre-60S_factor_ECM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022784">
        <property type="entry name" value="Ribosome_bgen_Alb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28280">
        <property type="entry name" value="SHUTTLING PRE-60S FACTOR ECM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28280:SF1">
        <property type="entry name" value="SHUTTLING PRE-60S FACTOR ECM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09135">
        <property type="entry name" value="Alb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="188" mass="21420" checksum="7BC5F4520B70E5EF" modified="2017-01-18" version="1">MAKKGLSKNSRAARRGDVNPFESSEAKELASLPRLEKTDTVSPLIRSSINKNQQLLNDRMTKASREGSKLKPGDNIVTMHNSKKKQHMVSKTIRTKTQRFQNVDGRLGRKIENALKRKKTIANLRKTGWDKINEIAKNSLSNDLVSQIDSEKKTDTDVEEEIIDEMRDDDEPLVVENKNPFGFLEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK76</accession>
    <name>A0A1E3NK76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_24762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017634.1">
        <property type="nucleotide sequence ID" value="XM_019162367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK76"/>
    <dbReference type="STRING" id="763406.A0A1E3NK76"/>
    <dbReference type="GeneID" id="30179054"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="43"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="278" mass="30325" checksum="8CDFC3550445D170" modified="2017-01-18" version="1" fragment="single">KPASTKGYTVTTQGAHNITMGNANPHQIPKFDDPYKEREWILEHMAGVFRVFGRRGFTEGSAGHISVRDPVDPTTFWINPLGIHFSLLKASDMVRVDEEGNLVGGNTSAPINAAGFAIHSALHKARPDINAACHTHSKYGKAYSAFGKPLEMINQDVCTLYNNHCVYSDFGGVAIEKEEGEAIAKAAGESNRCIILQNHGLLTTGRTVDEAGYLYTLMETSCEVQLAADAAAASGKEKRLIGDAEAAYTAFMNGDPETLYMEFQNDLALEKHYDDSFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKA5</accession>
    <name>A0A1E3NKA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein BZZ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_16415</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in endocytosis and trafficking to the vacuole. Functions with type I myosins to restore polarity of the actin cytoskeleton after NaCl stress.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BZZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017664.1">
        <property type="nucleotide sequence ID" value="XM_019161197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKA5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKA5"/>
    <dbReference type="GeneID" id="30177884"/>
    <dbReference type="OrthoDB" id="8783038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11912">
        <property type="entry name" value="SH3_Bzz1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11778">
        <property type="entry name" value="SH3_Bzz1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000060">
        <property type="entry name" value="Actin polymerization protein Bzz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035459">
        <property type="entry name" value="Bzz1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="9">
        <location>
            <begin position="4"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="500"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="578"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00054085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="636" mass="71401" checksum="65C1DE88F4861AF8" modified="2017-01-18" version="1">MEDISIGNEIKDGFKRTDKWVKANIGFISEMESFYKQRALIEREYAEKLQKLTTESFQKVSKLSTTLSVGEEPTITPGSLECASVVAWKEVLVQTENIAKEKRELGSNFETNVAASLNKLQSKYSAIRDRWKGFDDELTSIRDKNYSEMSTKKKHYDSCCESMESQRSKSLKSSGERAQEKLAKRESEMNIAKNDYLITISVCNRLKDKYYYQDTPELLDGLQDLNEAKVSKVNSIWLSSSILEKESNEKISKFVDAIDVVVKQNLPKLDTAMFVKHNMANWVEPVDFNYIPSQIWHDDEKMIVNDATLTSLKIKLNNACSTYETYTSICNDEKQSVEELLVQRNKLVGDTFNEVQIKSPADYQCFEDLLIKSIQNLQKFTNDDTKRVMAEVEIETIQGSTGDIDMTITTPINKEKKSKFGFLKFKHDQEEDAEPRIQQLSESVNNISIEPSNRKGRMFSSLGKLVDAYNTPVNQRAPSLNFSNGGTGSISASTSTLPAGGVTTATALYAYEVTGDDELPMASGETFTVVELDDGSGWTCVSNSSGSEGLVPTSYLKIEEHRQQKKQGPKVSPRKNARLVKKMKILYDYEAQGDDELTVNAGDIVVILNEDDGSGWTEGEIDGYTGLFPTSYGKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKC6</accession>
    <name>A0A1E3NKC6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_34101</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017707.1">
        <property type="nucleotide sequence ID" value="XM_019162864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKC6"/>
    <dbReference type="STRING" id="763406.A0A1E3NKC6"/>
    <dbReference type="GeneID" id="30179551"/>
    <dbReference type="OrthoDB" id="5424147at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF12">
        <property type="entry name" value="MOZ PROTEIN REPRESENTS A CHROMATIN-ASSOCIATED ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="222" mass="25776" checksum="08DF3B5678CAA53B" modified="2017-01-18" version="1">MESFFDSYKVVQDFKNSGFSDAQSLLLMKYTYYKLQKKLDWLNQTYAPKVDLENEAYLFEAAHSELLFEVTNSREISLMNLTNAMIILKRAFNNLDDETIAQIKLNDDIIKMELNQFKHENNLHQKSLNLKNSDLNSRILSDMVSGLKSEIETFRWQLTRAGILSIMIMAVCIMGVWNLTKKLAIGDDAEHEYKGPLLQPVHQQTDEESHDYEADWDEPVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKH5</accession>
    <name>A0A1E3NKH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72677</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017733.1">
        <property type="nucleotide sequence ID" value="XM_019164271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKH5"/>
    <dbReference type="GeneID" id="30180958"/>
    <dbReference type="OrthoDB" id="3997535at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="261" mass="30052" checksum="FB2C83F14B3F5FA9" modified="2017-01-18" version="1">MVDINADKLSGRKYYKEHIKGLLDDTLRRVVENIEADRTYVRRNQPEPWLVTVPRICSFIATVVFTLYKADAGFTDFFNIGYGVKRKTVGDMVEWIETLLEARFHTSPPFTILHFVETMVFLTEGLDRPLERDLTFDYDGLKFYRKKVNNLVCDEQCDDEVMNMYYEEQETCEYSTNNIYAVKYLRALINILSVESSVDEVMNDLDSFRSQNALRIEGVGVPPPVNDDGSANYIKLTKIPWHDDASVLPGTTNEDDGKDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKI0</accession>
    <name>A0A1E3NKI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17298</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017181.1">
        <property type="nucleotide sequence ID" value="XM_019161535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKI0"/>
    <dbReference type="GeneID" id="30178222"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056998">
        <property type="entry name" value="Asd-4/GZF3_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25026">
        <property type="entry name" value="Asd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="9">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="405"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="494"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="557"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="660"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="572"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="758" mass="82643" checksum="CD81DC2C9071C628" modified="2017-01-18" version="1">MEHLASTSIDSGNPGQNPALPPVVMAPSGPQATAPAQAPGTAIHLPPFSVPSGSSGAAPAAPAASQQSPRSNHANSHQHSQPTIKSEVSVKDEFRDEPQFPETHSQHLSPSGSNQDNNSSSEDGSKKDLDHIVPNAKTTTTTQSNGSTTTKQNMSTPTCKNCKTQTTPLWRRDETGQVLCNACGLFLKLHGRPRPISLKSDVIKSRNRVKHNNKSSPNTPELKAKDVNGSNVLNGTHNNTNINNNNNNNNNNSTSGNHSSPALPSNGSNLPNQNGMQSINNSSNNKISSKFLPKQYKPNTKNSKSKRSTKSDSKDQPSKAAKTNKSKQLNHSDGVNGNITSPNLLPLLPRGQATQNLPPGTPYTYASTTAWMNKSQGTNIQSLHYPSSTPTQYVSNLNRITSPLLLSTTTPPKLNPTPSQSQSSERSSSMNSVPNNLNRDAVLNAAGALEILSQQATSTDYIPGMRLNSPHSTPLSNPTAMVEKLPPIGLLSRSQTSFNEHDGKSSSNEPTHLHNILSPPNTDIKSPSPVAGINLLQQQRHYQAAEQIHTTLPPLTAITQQPPLPQQHQHHQQQQQQQQQQTQQAQQHQQQQAQQSQQHQLLEQSQSQQQLNKLPPLKNVPGISSVMYNNDETNSKVHGPPGSASASADVSLLNINNMNNSNLHDENERLRTRISELELVNDLYKTRINELEEVDAKFQKAESEYRKQIRDLEYSLGLQNQQQVNLKMNSEFSGEKRQASNDEAVNAGHIEKRIKVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL57</accession>
    <name>A0A1E3NL57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72884</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017964.1">
        <property type="nucleotide sequence ID" value="XM_019164318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL57"/>
    <dbReference type="GeneID" id="30181005"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="371"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="583"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="621"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="655"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="274"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="82"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="684" mass="75571" checksum="82195099A33241DF" modified="2017-01-18" version="1">MSTHCDKDIRPQGDKKSLGFGDDEKDHDDKTAKHNSIVELTNVEDLVEKEDPDRPLIIVPSHKTVSMQSRLQSLLAQQAEREKKEIAEKESELAKENELLGFGLNEPSKPVEDTAEKPSTPSTSSTDTLSAKLSEIGGEEPTDESYSKVPVSKADGGSRRGSRSSQSLDDDSSRTGIPRAHVKNHSKRQQEGSLSYRKYSEFTRRKDGLELDELTIDEDSFRRNDAGRDRTGRFRSVDGDGTGDEEDISIDNNAAAGTDVDVDADDSLYPKGWRPLIVVFGSFLACFTLFGVMNAIGAIEAFVQENQLASDSVSSVSWVFSIYMFVSLFLGLLVGPLYDTFGATYLLLTGSILTFAGLFSCGSATTIYQFILSFGLCTGMGTGFLMFPAISVISSWFNRVKRPFYIGVAQTGGSVGGIFFPILLRYLFDKYGFTWAMRIFALFNLGVTLLSVLLTKDRLKELRDLTGEAIDERTFWDKLKGSIDLNYFKDKKFMVLTSALFMNEFSLLIVLTYLASYAMAYGASESESYVMLTILNVAGTFGKFIPSYFAQKYGCFNMMILMSVSMTLECFIIWLPFGKHKAALYVFIVLFGFAYSATYSLTGATVGAITSKTKDFGKRYGSAYAIVSFGNLVSLPISGAFIVNKTTSDYDHMVAFASASCCVASILFLFSRYTIVGRKWKVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLD3</accession>
    <name>A0A1E3NLD3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA polyadenylation factor FIP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_139268</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018044.1">
        <property type="nucleotide sequence ID" value="XM_019160136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLD3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLD3"/>
    <dbReference type="GeneID" id="30176823"/>
    <dbReference type="OrthoDB" id="1917198at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="7">
        <location>
            <begin position="150"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="273"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="366" mass="39648" checksum="E34693590523AE51" modified="2017-01-18" version="1">MDDDGDAFLYGSGNENEQANVDTGADAGKAADAAASTDTVLEIKDGVPSTTQLDASDNNTEGQGADNDNDGPEVEESESDSDSDVEFIIGSLESKVNIPAAQLKTTPEGEAPVGTVADTTQVTTVPIFTKSTGLDINRVGLYNDIPVTQLSLDELKDKPWRLPGADISDYFNFGFDEISWRAYCAKNEKMRNDFNPGKVITELLAVGSIPPTLSQIMPNGAPGSGSTPGSMPPPFMMGMPGFMGMPNLPNMPNMPNMPNMMNGMNGMNGMNNMNNMSNMNNMNMNNNSNIPNIPNIPNINSRTSSNTPDPTNTKLPHIPSALRDARDQADKDLEKGGNNQKKFKDRERDKDRDREGDRGGRHRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLD4</accession>
    <name>A0A1E3NLD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72954</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018043.1">
        <property type="nucleotide sequence ID" value="XM_019164342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLD4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLD4"/>
    <dbReference type="GeneID" id="30181029"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF3">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="125"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="411"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="524"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="638"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="692"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="224"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="447"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="638"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="707"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1192" mass="132036" checksum="B4D1B66E1059492C" modified="2017-01-18" version="1">MQSKRTKKVGITGKYGIRYGSSLRRQCKKLEIQQHSRYDCSFCGKKSVKRDATGIWTCKSCKKSVAGGAYTVSTAAAATIRSTIRPQFHHFEISVFPTNCFGLQSLFTMKKSQIRANLLDETAPFVQPSGQTSSYSKHQHSQGYMNNKSQPQQQLPPQQHQQMQYALHPQQQYQQYPADPYNGSRNSHHPQQRQALSPPQPQPHRQHHSASYGTNPNPKYAYNYDQQRPLSQNHHSYPVNHAPGPDYQGGYQEGYDAHPQYMDQSRVSYNGPNGPYQPPPQSQPYHDPYQAQPRSHSHSYNQPSHPIPQARSQSESYPQTGIPPQQEPLHPSQQLFTVEDSDGYGNTTKSKSNKSGKSKSRAINVADGDNDQEVPSTSFEMIARTTSIEQDKIQQLEEKIQRLERVLALQELESKTSRSSTSIDKVPDIASEPQQQQQPAPAPAPAPASASAPALPTPPAKSTKTATKSASKLPPLTNTGAPPLPPPPPADKKSLLNSPKANLFLSGLDSSSKGSDDLRLSASSLIPTEKSLSDLSDGDGDHSIELNDEEPPPSYEELEKSGSLTYSQSIYRTGFEKAGYQMDHVITPTNPVAESSNETTKSTGKITRELMSKRKIPSQITDEMFEKAAFKTSLSSIPTLSSETPAKTVTESSTSQLKSSVTAPSPVQIPSQMSQVPPPIAAKSRNSLKNHVTPFHSEFKPPARLENSASATNSKQSRSTMDSSLYTPKATTSELTNIIKYSTDLSFESLANGVIKPILPSQSRESTEETIARFRKTREKALADYKQFTPRVQFFWAILLVETLSKSEVLSKMTIDGKLRKNPVSYKKLKKQRLMFLTTAIKVLEKLIQIAPNDTRARLYLGDIYSGGIHPGLIERDEALGYQLFYDAATKQNDPVACYRIACCLESGVGCKQDISKSIVFFERGATLGDPSAMCQLGMMHFAGVNGCVQDISLSIAYHKQAYETLRSRTVMGFDPLISSRSFQDARGALYTLAKLHQTDRQILCLADGNDDKTRKTIEELKFANVWCNSSKALKYYLEAAKLDHNESQACLGYYYSQGFFPTYSFKSDKEANDGVPNTLDARKSIYWFSKAAADGHTYASLGLARWYGSGAVDGEGKVILKRDEQQAFLWGRKAADAGELAEAEFMIGVCFEQGFGVEKNAVMAANYFERSGRKGYKKAIQKLKAMKNATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLI7</accession>
    <name>A0A1E3NLI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73009</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018107.1">
        <property type="nucleotide sequence ID" value="XM_019164358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLI7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLI7"/>
    <dbReference type="GeneID" id="30181045"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000031">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="226"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="384" mass="42682" checksum="30D6A1CEF5400B15" modified="2017-01-18" version="1">MSLVNICRDNNDPFYRYKMPLIQAKSEGSGNGIKTNILNLSEVATALARPASYVMKYFGFELGAQTSIDNKNERYLINGLHDPEKLQDSLDGFIERFVLCPQCKNPETVLEIAAGENLQRDCKACGKITKVNPSYKLVSYILKNPPKDASKKQKKSASGKTASANVIGGGKSISDIANRQQGSGSGSASGEDKPAEKVVVKDEDWSVDMSEEAIKARAKELEKLNIGEARAKYEDFGKWLLEGDDGELPNDVEIYKKILSLDIAGDRQTVEVLAQVLFDDDMIDQIDAHKGLLLKLVTDEKFEKSLLGGIERTIGTQHPDLVKQVSKILMALYDEDLVSEDVIRDWGSHVSKKYVDKETSRNIRKAARPFLKWLDEADDESDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLR2</accession>
    <name>A0A1E3NLR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71187</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018177.1">
        <property type="nucleotide sequence ID" value="XM_019163854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLR2"/>
    <dbReference type="GeneID" id="30180541"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF20">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="516"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="559"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="627"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="687"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="10">
        <location>
            <begin position="787"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1043"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1046"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1094"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1124"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1149"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1164"/>
            <end position="1174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1196" mass="134831" checksum="608C2A55834956E2" modified="2017-01-18" version="1">MSSSIVEIKQLFKISQEGSMHVANARGISLESLMASLMFSTIYCVLQLLLFYYLQSRYPQFYDKYGNNNKSISSSNFAQSSPNKSKMSFVAFCLHHIRKKNDIIRFLDIEDYRKYGLDSYLFLRFLYVCLYFFVGIALVCLPVLLPVNYLTEANVNSGLDLISILNIPSNNTYKYIFHFLVTIFIVFWFHYILVHEVNFFIKLKFGFYSSMKKYDSGNRFLKTLYFENINTEKYRTVSDLKKLLESFIPNCIEDIYPVYYSARIVKMVNRYRKLRNELERHYSELAKYKTGERQSQVKSMMYLPIKILNSDYEITVVGLSYKVNKCNYLQSEMKHLVQEIQLMRLVHLQNETLEDPLLVNDKVFIVFKNISFKYILQQVQLSIGKDCKMANFKMADISPRDIKWDKLVAGDNNQNFFLSIKECVLFFISVVVIVCWVIPISCVGTISQLNCLTMLIPTMRWINRIPQVLQDFISWVLPTTVLTFLTSFALVIFRVISNRKRHITGASKEMSIQQWVFVFLFFQLFIVITISSGFIVVLQKLILNPVSIPLILAIDFPKASIFFTSFFILKGLTLLGNNVLQFYYFVKKCFIDDRLLGSKKTRRELHEAELRNKYELENYWGQIYPTFSVYGCIGIVYSVISPVILIFCCINFTLDLLGYKYSIKYTLNRTNPSETHGKLYINAIKQLYAGIYSLEVFDIGLFFSIRDSNGDRSCIALSVLLMIVLAITVYTHLHLNGEHEGLCELLLGTITTATTTHITDDALASAEGSQIENTASATTLSTCNPLSATSCSPNPALATSVSDDFKANSDNYIPYRTPSQIFYGDDGLTLTLAKRYDNPSLVSDFYILFGKIEVWLKSAPGTGVISSFYLQSDDLDEIDMEWFGGDVSQMQSNYFSKGNTTTHDRGEYHDMADPRAEFHNYTIDWDESALSWYIDGNLVRTLANTSSEGYPQSPMRLFFGIWAGGDPSNNEGTIEWAGGETDYSDAPFSMAIEKLVVSDYSTGKEYEYSDTSGEWSSIKAVDGEVNGRKVIAVAEFASLVSGNKDASNSIDSVSKSSAVSASPSASSTSKSESESHYSSDEATPTSKSEKQKSLDSGSSVTTSAKPKTTDTKETSDADSTGTEKTVSLKSKYLSSSTTTLHTATTSKSKKSDDSSSSSKEEKSASSSVSAHSNAANSKIYAGSSFAAALMLMLSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM11</accession>
    <name>A0A1E3NM11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="6">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_57728</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Vacuole membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018292.1">
        <property type="nucleotide sequence ID" value="XM_019163255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM11"/>
    <dbReference type="STRING" id="763406.A0A1E3NM11"/>
    <dbReference type="GeneID" id="30179942"/>
    <dbReference type="OrthoDB" id="1735853at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902906">
        <property type="term" value="P:proteasome storage granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="8">
        <location>
            <begin position="33"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="7">
        <location>
            <begin position="153"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="9">
        <location>
            <begin position="385"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="501" mass="55897" checksum="C9CA02BE9C625C9B" modified="2017-01-18" version="1">MLSDKELFDLNKKAVTQGFNIKPRLDYNTVGGVNGPLVILENVKFPSYNEIVDLTLPDGTTRAGQVLEVRGDKAVVQVFEGTSGIDVKKTRVEFTGSSLKIPVSEDMLGRVFDGSGRPIDNGPRVFAEDYLDINGSPINPYARIYPEEMISTGISAIDTMNSIARGQKIPIFSASGLPHNEIAAQICRQAGLVRPTKDVHDGHEDNFSIVFAAMGVNLETSRFFKQDFEENGSLERTSLFLNLANDPTIERIITPRLALTTAEYLAYQTERHVLTILTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLSTIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQISVDRQLHNRGIYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYAKYAIGRDAAAMKAVVGEEALSTEDKLSLEFLEKFEKTFIAQGAYENRSVFESLDLAWSLLRIYPKEMLNRISPKILAEFYDRERNDEDEEEEEEKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMK2</accession>
    <name>A0A1E3NMK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71413</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018444.1">
        <property type="nucleotide sequence ID" value="XM_019163914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMK2"/>
    <dbReference type="GeneID" id="30180601"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="329"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="532"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="771"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="800" mass="90521" checksum="3795A1C597825862" modified="2017-01-18" version="1">MSLVFDQNSKSRKKTINPVIENLLKEPISFQLAKYPDKVSIKPSSYTVFKGYKKGKTPDASAAASPEPNTRPEPERMTRPKSLAEALANYAGQKPAEDADSGQPASKKKKASTPKADQKQRLKKLLLQQSKGSDAITSEEDETDEKVRSTDEPELAEIKNEEEDDDEDNGDEDEDGQEEDDDDDEDDDEDFHTAESEEDNDEEDDDDNDGEDESVSDEEDNDDGESNNNDNEEIDEESLRGSSDVIDPLKKDLKEDTLTSKVLDNQPVDTPPTSNNDDDEYNDKAQLINTEYPNQDFWDLSESPNDHGTNDPKRIDKSWGDFIEGHTSLGLLNHGVTCYMNAAVQSMCHIPSLTHYLIDVYKGKYDDKIAPDSVTKVLASTVAKMYRIGHNKKIIYINSKKLCKRLNDINCMMSEWQQEDSHEYYMSLMSRLQEDSTPKGVKLNKSIIYDIFGGLLDQTVTCKNCGHISTTQQEFYDLSLGLDSRKKRTSLMVDPQQLYQLRDQIEKCDSSSKEAKIQLSKILQQRILLAQEESNDSKSPEQEEENSSSNGKNTQEQNESRDKDQTPGQKQDQLSADKPNSHPQSQSSQQEQPSSMAQNASRKYLLENSIRDFFTPEIIKTDKRDQSGYVCEKCNIKTNAIKISTIQRSPETLSIHLKRFRFNGQQSQKVKANVKYPEILDLTSFTTDMSTPTRYKLTSVIIHQGRSVSSGHYIAHCRQPDGTWATYDDEYINKIPRASALSDPGAYVLIYERLTYKTANGEVDEDKLVNNLTTRKRKRVESGSSSPLNPKAHAKSRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMT0</accession>
    <name>A0A1E3NMT0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_22575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018031.1">
        <property type="nucleotide sequence ID" value="XM_019162268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMT0"/>
    <dbReference type="GeneID" id="30178955"/>
    <dbReference type="OrthoDB" id="5419460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414:SF3">
        <property type="entry name" value="SUR7 FAMILY PROTEIN FMP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="231" mass="25856" checksum="CD58EBFBFEF3493B" modified="2017-01-18" version="1" fragment="single">MNVLRIFIIFFATIFLLGSGLTLFLTILTGATQTSVLRKFYWLETDCSNFSGSPVSSTCRWTNYGLCGVQDNRNTDCTHDVAAYPFSPSRNFNSNDNLPSAFVDNANYYYYTSRIGYGFSLVGLAFLVFSWFPFLILLFTKVGFAALQSVFWVLYGLALLFVIIGIALSTSAYAKGRNHFRNAGFTANLGTKAMATAWTTVFLLLFSIPFIVFASREWSLGSLGRKRRGYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMW0</accession>
    <name>A0A1E3NMW0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_112969</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018539.1">
        <property type="nucleotide sequence ID" value="XM_019159776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMW0"/>
    <dbReference type="GeneID" id="30176463"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="258" mass="29073" checksum="CF10CD92366F852D" modified="2017-01-18" version="1">MMYLVIPTKQRTRSYENLNLKKWPFFRNLVLARKFTMGTFPNVIKRPGQEGVLHRNVTDHIREKRWTQWPTSLSCSALLYSALSPVRCFSVLFSQACSLTPPLHTSAAAAALPQLTTFSMGSSLIQDPARLHARPSVTSKSIPVQAPQSVQAHSAEATVKPFRPHSGRNLHIRRRRCRMETRPRLFPRPRPGPRPRPRPGLPLWGEEWVAGQMAALGDSSRTRENVHYGHRPGPVEKSKTLLLYAVLGAAVSAVWSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNH1</accession>
    <name>A0A1E3NNH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromatin modification-related protein EAF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15576</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MRG family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018764.1">
        <property type="nucleotide sequence ID" value="XM_019160876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NNH1"/>
    <dbReference type="GeneID" id="30177563"/>
    <dbReference type="OrthoDB" id="124855at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF15">
        <property type="entry name" value="MSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MSL3 chromodomain-like" evidence="10">
        <location>
            <begin position="9"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="MRG" evidence="9">
        <location>
            <begin position="217"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="112"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="395" mass="46211" checksum="DA31BC294B89DF91" modified="2017-01-18" version="1">MTEKVYLPGEKVFVYQGPLLYDAKVLKTFDPVTQKVESFDEKKKEQVYSAPNKKFPQKFLSEVCYMVHYQGWNAKWDEWVLPSRILEVNNENLMLKENLTFEMQEQERLQREALEEEDRKKEKEKTKLTIKLQKRIPGDSRRKSSPSSSEKPEKNEDSGILSIINGNGNKKGRGKTKTGNLKHKDDESRDRCSSDIENSVKRKRLNGASSKNEANHYESKTGFTHHEIKTLVPDALKIILVDDWENITKNNKLVSLPGKCSVSKTLDDFMLYLQREFSEEVSELDIFLEMTQSIKLYFEQCLGTFLLYRYERPQYSDILDKENDSHLYDIYPSIFLLRLLSIFPNILVMNNVDPGTVRISKVYLDIILFWLDHNKGTYLVEEYENQSPWIALMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNL0</accession>
    <name>A0A1E3NNL0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018206.1">
        <property type="nucleotide sequence ID" value="XM_019163863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNL0"/>
    <dbReference type="GeneID" id="30180550"/>
    <dbReference type="OrthoDB" id="10034502at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013744">
        <property type="entry name" value="SidJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591:SF1">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08538">
        <property type="entry name" value="DUF1749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="296" mass="33023" checksum="C98E528F32289EE9" modified="2017-01-18" version="1">MSFPVAGLIHEYAPRHIAFEFAPAAHKNVVIFIGGLMDGLLTVPYLQRLAEELAKINYSLVQIEFSSSHIGWGTGSLNRDSTEISQLVTYLKSEKGGKRSTIGLMGHSTGCQNTTHYFTRQERDDPAKYSAVDFGILQAPASDRASATMMMSKEELEESLVIANDLIAQGRSDEIMPHKFIKRFFNVPINAYRWNSLMAVRGDDDFFSPDLEDADFATTFGKFDRPFLVLYSGSDEYVPKWLDKEALLDRWAKVASVNWSQYSTVVEGASHNIGEGSTNNAQKNAVDTVIKFIQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNP2</accession>
    <name>A0A1E3NNP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein bL21m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_26189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018825.1">
        <property type="nucleotide sequence ID" value="XM_019162437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNP2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNP2"/>
    <dbReference type="GeneID" id="30179124"/>
    <dbReference type="OrthoDB" id="5994at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="bL21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="bL21-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="L21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="137" mass="15150" checksum="D76624F3BFC58199" modified="2017-01-18" version="1" fragment="single">RSLTTASSAAAAAAKKPVNSLLPLKFEQNLYAAVKVHNRSYLVTKGDLVNLPFCMHAAEIGDTIHFSKIDTLGSRNFTYHVADGIDADKVTVTGVVVEKTKKPMTIKEVTKRRDRHIKHSLSKHDLTVVRISELKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNZ5</accession>
    <name>A0A1E3NNZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018927.1">
        <property type="nucleotide sequence ID" value="XM_019160925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNZ5"/>
    <dbReference type="GeneID" id="30177612"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="235" mass="25893" checksum="535C4742E925D6A4" modified="2017-01-18" version="1">MCLDADSPTHSASPSPLPLSLSLSHCLCGVGLPSPTPSYTSTSTSASSSASTSTALTPTTKPLVFVTNTVHRSRLQKPRDVTFIIRAFVKYVQQARPPPAADAVRNTAHTNALLLDRHMHDFLTTLNIIGYNLPARPVSYSVRITLAFHGPFLLPRRARRPHTPSAPRYRCHAHIDLAFFSTRPVDPAAVADLDRLVVAYFVHNPQLLRFARLHLPSPWILNTVHVNTACFDAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP01</accession>
    <name>A0A1E3NP01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_22543</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018939.1">
        <property type="nucleotide sequence ID" value="XM_019162266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP01"/>
    <dbReference type="STRING" id="763406.A0A1E3NP01"/>
    <dbReference type="GeneID" id="30178953"/>
    <dbReference type="OrthoDB" id="62560at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_bS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_bS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_bS16_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919:SF20">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="Ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11632" checksum="B8900C4C65C50B66" modified="2017-01-18" version="1" fragment="single">MKGLVRIRLVRKGRTRQPVYNITVGRKRASLQSVPVEVIGTYNPVPTPISPSMKAQGIIPVKDVKLDFDRAKYWISVGAQPSDTVANLFKKAGLLYEQWPTPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP05</accession>
    <name>A0A1E3NP05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_19118</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018932.1">
        <property type="nucleotide sequence ID" value="XM_019162107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP05"/>
    <dbReference type="STRING" id="763406.A0A1E3NP05"/>
    <dbReference type="GeneID" id="30178794"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16456">
        <property type="entry name" value="RING-H2_APC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024991">
        <property type="entry name" value="RING-H2_APC11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12861">
        <property type="entry name" value="zf-ANAPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="35"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="81" mass="9159" checksum="380BC3507C925FA1" modified="2017-01-18" version="1" fragment="single">MKITINSITPVYSWHWSVPDDELCGICRISFDGTCPNCLYPGDSCPLTIGTCHHAFHLHCIEKWLQQSSSRGLCPMCRQAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP09</accession>
    <name>A0A1E3NP09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BFR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_31619</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018934.1">
        <property type="nucleotide sequence ID" value="XM_019162711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP09"/>
    <dbReference type="STRING" id="763406.A0A1E3NP09"/>
    <dbReference type="GeneID" id="30179398"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="5">
        <location>
            <begin position="189"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="4">
        <location>
            <begin position="414"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="103"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="532" mass="60841" checksum="229E36170688E430" modified="2017-01-18" version="1">MSVKGSKKKMLSDRIVAKLNKPKIKDYDIEDEDFNYENGSSSKPDGFNEEDDENDDEEEEREREELAKRDHYVKVSESKIRGKSLNLGEKYTGSKVGRSELYDNGDSESSNSEEDSKEDDEDDYEKNDEDILADVTDSEAENANEENSEEEVEDEEDEEEEHKRNRIARLLDTEKQQIFNRLSTSAKTDTLKGYTILRQGAEYDKILDARIKLQKAIISGNSLPIDNKVFEENKTDQTDSIIEATEGKLYGLIDRLIRLRAKQLLRDDLVKEKVKVNLKKRKLSSYLEANSSIDKACAPIRKSVLMKWSSRVQSASGIGALNQGKFSVINQNVWTQVNNQLGDLDRLIKKTKINRRGITPMGYSEALEKKLEDEVDADDNDDSDSGSNFKTAGLSNIDKSLQTNQYIFDDDDFYRLLLNDMVNKKLDQKQANNAAILMLSKNKMQKNYDRMATKGRKLKYTVQEPLIQYEIPRRKYYSWGDDQIDELFAGLFGINLNINDSDNDAEDDIDAEQDGQKDDITALRESGLKLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP30</accession>
    <name>A0A1E3NP30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018396.1">
        <property type="nucleotide sequence ID" value="XM_019163905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP30"/>
    <dbReference type="STRING" id="763406.A0A1E3NP30"/>
    <dbReference type="GeneID" id="30180592"/>
    <dbReference type="OrthoDB" id="9932926at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006993">
        <property type="entry name" value="Glut_rich_SH3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04908">
        <property type="entry name" value="SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="597"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="885" mass="97039" checksum="B68A865F5B2ABF02" modified="2017-01-18" version="1">MDMNIEVEGLKSKNTPSLIGSSLRDSSDQIPTLKSFNSDSTKASFVKSESASELVPVDEIAKEGGLTTDQEEQELNDVLDREGHVKKNLIPDAVMSEELKSNVSTTSLTPAELKDLNVEEERIDDGNKETILGGDADKYLAPAGGTSSDYKYPLKKKKGKDLTQLEDSNLFIEPDKMRSVDDIINVVNKGNFEKASTLIDGNVVKSICPSTKSLSLEEVKEVEDPPVPDSAIEVDSEFTPPAAMREGSESRLLEEQRSRSLSRHRHSSSRSNDHGDRPNLARGESIHSGLNGDLSITSTYDKSSHSLVSERRPRHDPSGPRIPNSSSLNYLRSISRSRSRMANDRKALGEEQLDSNDLRESGALINDDSMSNRSDIEYAVNKALDFVEDTHSVKGGKRLSDGGDIVKNLQQGLAEVAEEEQASNRKNLSSNDLLDQLATSAMELMLEDDEEEEATKSEKSGRADGANDKERGTALTDSNTDEPASGKTIVEDEKCQGDQVENGDKLTSELKIEDGISENEKDENEVKESNNDVPDIVENKTNLSEENGSGVGATEVKYSNEAEIDGKIDAKVSEQEAEKETEIPKHTGEGEGEEKLIQSNVKGTSSSYSEDTSGAIKTEKKDTESVENNEVLGKPENSEWPNPQKISTGQKQLADTEVEADGVENPVELNESLSVNDDFSNAGSEEIKKVVGEKEEMNLIEETTNDGEVGSTEAGKVNLESSKSPSVAENTEVATEPSHAPTAEIVHSHINDADDTDAIIAAAMREKAMKESYNPLGKDGSVFVPKVEKMTFEDEPVFLYTSFAGGFQVTTRTNRLITILTANRVKFEYRDLGTDEEAKKVWRRHSAGKTLPGIVRGKDDFIGNWQDIEEANEDYRVRSMIYETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP37</accession>
    <name>A0A1E3NP37_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_120191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018964.1">
        <property type="nucleotide sequence ID" value="XM_019159870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP37"/>
    <dbReference type="GeneID" id="30176557"/>
    <dbReference type="OrthoDB" id="3997549at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="316" mass="35983" checksum="E95771323B920E91" modified="2017-01-18" version="1">MFCEHHCSHMTDSPQLEEYVKQRDLLLRRRDELRRRRQTSPAHVQRHKRQKIETVARTLDTYESSKTLISKYSRLPEMDMGLRLQYARMTCPWMSVVAVKKDRLAPDTGNTTIRCETTLRFDSYGDFVVTLEIDAEGEDVRRLSITPSPDAEEVDEQKAISVLVAESTQTLDVSRLVYGMNTLLRMRHKRKRAWMAIVDELELGKVRAINGFAVGATPSQQRDSLGRLLFDHSPSSVEFELESGKSLRVVWHIFFRPGTRHCVSDFTASVVAAAADTPQDAEGGSSRPVENITRLLKSLIRANDIKLATLQVLNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP42</accession>
    <name>A0A1E3NP42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_76882</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018979.1">
        <property type="nucleotide sequence ID" value="XM_019164952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP42"/>
    <dbReference type="STRING" id="763406.A0A1E3NP42"/>
    <dbReference type="GeneID" id="30181639"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="247" mass="28123" checksum="72568ABFB64B9F97" modified="2017-01-18" version="1">MSSAHRPTFDHARGKEKKVASTISHKRALPSHSKLKFRSKKARNNKEFYNYSKEETGESSYNVNQELLEPLRSDIQKTVQTIETRAKEEVDSLNERVGDATSSDGKGEGTENEVTYESARVGENHDEEESAAESEDESQSGSETGSDEDEDEDEALLKEIENIRKEREEAKRKAEEEKIEQRAMSSNPLLSVEGSNEGVAPDMKPKKKSWRSVKTMSKKPNMSHTDRFSNDTLKSDFHKDFLDRYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP55</accession>
    <name>A0A1E3NP55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_32306</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018984.1">
        <property type="nucleotide sequence ID" value="XM_019162756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP55"/>
    <dbReference type="STRING" id="763406.A0A1E3NP55"/>
    <dbReference type="GeneID" id="30179443"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000014">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18910" checksum="25845243D7475EC8" modified="2017-01-18" version="1">MSAVQEQPVAKQTRKTARPQKLRSSLVPGTVLILLAGRFRGKRVVYLKSLEDNTLLVSGPFKVNGVPLRRVNARYVIATSTQLSLESLDLSKYNVAYFARERVSSKKSEKDFFGEGAPKKEIRAERIEDQKVVDKALISEIKKTPLLKQYLASSFSLKTGDKPHLMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPH8</accession>
    <name>A0A1E3NPH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_116688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018731.1">
        <property type="nucleotide sequence ID" value="XM_019159823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPH8"/>
    <dbReference type="GeneID" id="30176510"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="82" mass="8633" checksum="C074CFE89429A730" modified="2017-01-18" version="1">MHAGNACSMRDPPLAEDFSVPPMFHDPSGRPAAPLPSRFSPGCIGDPSCCSRRVLSAPVTGCRPRGLTIGGKKGYLVLLPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPI4</accession>
    <name>A0A1E3NPI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019088.1">
        <property type="nucleotide sequence ID" value="XM_019160990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPI4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPI4"/>
    <dbReference type="GeneID" id="30177677"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF589">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE [ACETYL-TRANSFERRING]]-PHOSPHATASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="117"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="539" mass="62303" checksum="E79B34468DD6D291" modified="2017-01-18" version="1">MFAKHIQKCQRRFISQHLTIHSTNSKSPINFHVDLLKVPSNFGYYSGRVNRPYNEDRWQASVLDLQPEALRAVDPFQGSSKQENDTFSEEKILDAFHGYDFDNLKLLKQTSRTSEPTYEKSSPPINRTVFSFGVFDGHGGTECSTYLKDHLFENIENINISEKAIEDMKKFYRSKINGYWKRWGRKIGDVVYRKTRAQTAVEKEKGPKQIRVDEQDNEEEEDNFIDWEEFRSGKKLWTVIEMMLNDNKLTHWEIFKLRLWLGYLFTDLQFLTWENEANKEKKTSTKTNESDAVQYRLVNSGSTCTSCFTYAVDWEENDTNHYFYQDNVVSRLVVAHVGDTRAIICDKYGLAHSLTKDHHPSNPIEASRLRRFSAGMIMTDSFGEERFLNFANTRSFGDLTAKDIGITSEPEISEYLIGNSRMIEKFKENSKNKQSLKDNDVQDFGGEESFVVLVSDGVTNNLSDQEIVDLIKTNFNNRGVNRGNPSKGAEEVVGFVECIGGDDNATCLVIRLGGWGKWPIEDRTGKLREERMMDTRYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPM5</accession>
    <name>A0A1E3NPM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_31998</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019193.1">
        <property type="nucleotide sequence ID" value="XM_019162736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPM5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPM5"/>
    <dbReference type="GeneID" id="30179423"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071256">
        <property type="term" value="C:translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046967">
        <property type="term" value="P:cytosol to endoplasmic reticulum transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="113"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="631"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="673" mass="76562" checksum="23CA67C2243344A6" modified="2017-01-18" version="1">MSQSRYEYDENSETWPYFLLTTILVPLVPATVSKIYSSVSEATDKESSTSFRSTKKWFKPYNDKEIENFKASRGRSKLFNKSNSLIALGWLLTAILFLKISSITLTVSETNFDPWKILEISDSATEKVIKTAYRKLSLKYHPDKVDTSSMSQKEIDAVDAAYVLINKAYKALTDDTVRENFLKYGNPDGPNEIKHGIALPKFLIDGPTSPFLVLVYVLLIAVILPLVVSSWWNGVKSKTKQNIHVNTADHFMKVMINANPSKMLLIDDVLEYVSHAAEYVEIDSTLSPKQVHSLLLSYINREPTKDERLRLNVVSITPKLISAFIEISAAFKNTDYCVKLVDAHRSIIQALNIDKDAKTRKFQQILQLPGTDISKLDTTQNILTLGKLLKKPTVEPSKFLGVEVKTVSNIQDYAKSIPLIESYECSFKVPGEDFVPPNSSSHINLKFITKSPLHKSKPELKDFAKNVLETQLKEEESMENLRDPLKFVEEQKDIVIDHLPPFFPDEDYIKQNCGWVAFLVAQRDNKIVEIPKMIRKADLSNMRLTQEQYIKSECQISTFKIQLQSPTPSAVGEYQFRLVIRNLIYFGSDIDIPLVMKVEDKPMDVQKDVYGIEDPDEDSIAGAMAQLRGEPVKKIEFENEYESSGDEDSDKEEELWTDIDTDTEVEEDAVDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPM9</accession>
    <name>A0A1E3NPM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_58081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018781.1">
        <property type="nucleotide sequence ID" value="XM_019163277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPM9"/>
    <dbReference type="GeneID" id="30179964"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="76" mass="8544" checksum="13CADC03C7345C4F" modified="2017-01-18" version="1">MAKQTLFWIHVSCLIRVRRPDAIPKTGAQEHCGIPTPYQSRDKTGRSSQIPGCSWLANDSGVITYYKCRTFQTACP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPN0</accession>
    <name>A0A1E3NPN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial</fullName>
            <ecNumber evidence="6">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_15889</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="6"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019160.1">
        <property type="nucleotide sequence ID" value="XM_019161007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPN0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPN0"/>
    <dbReference type="GeneID" id="30177694"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045242">
        <property type="term" value="C:isocitrate dehydrogenase complex (NAD+)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000003">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="26"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ48047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="356" mass="38278" checksum="EFDD32357233FF66" modified="2017-01-18" version="1">MSAQRQYSSKVAGQYLGTPNANGRYNVTLIKGDGIGEEISNAVVKIYEAAKVPVDWEPVDVTPSLINGVTTIPKPAVDSINKNKIALKGPLATPIGKGHQSMNLTLRRTFGLFANVRPCKSVVGYKTPYDNVDTVLIRENTEGEYSGIEHIIVPGVVQSIKLITKKASERVIRYAFEHAKSTGRHEVIVVHKASIMKLSDGLFVQCAKEIAKDYPEIKVSFELIDNTSLKLASDPTTYSKVVMVMPNLYGDILSDLSSGLIGGLGLTPSGNMGEKVCIFEAVHGSAPDIAGKGLANPTALLLSSCMMLRHMSLNDYADRIETAVLKTIASGPEHRTGDLRGTASTSSFTDSIISNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPN9</accession>
    <name>A0A1E3NPN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Translation machinery-associated protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_117432</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 6">Involved in translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the TMA20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018791.1">
        <property type="nucleotide sequence ID" value="XM_019159835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPN9"/>
    <dbReference type="GeneID" id="30176522"/>
    <dbReference type="OrthoDB" id="10249667at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11609">
        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21155">
        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.20:FF:000001">
        <property type="entry name" value="Malignant T-cell-amplified sequence 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="7">
        <location>
            <begin position="91"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="180" mass="20442" checksum="BA439D49673FBF65" modified="2017-01-18" version="1">MFKKFTKDDVHTRTNVKASVSKTLKSKLVTQYPKLEEYIDEIIPKKVPVSLFKCEDKIQLYVVDNQVLFYQQFDDLIPALRVVHKYPEAFPRVQVDRGAIKFVLSGANIMCPGLTSAGAKLPDEDWPIDKIVTVYAEGKESAMAVGKLAMSIHDIKTINKNTGIELYEYLGDGLWHFKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ7</accession>
    <name>A0A1E3NPQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VPS4-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019135.1">
        <property type="nucleotide sequence ID" value="XM_019164077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ7"/>
    <dbReference type="GeneID" id="30180764"/>
    <dbReference type="OrthoDB" id="2158714at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013640">
        <property type="entry name" value="Vfa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218:SF1">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08432">
        <property type="entry name" value="Vfa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25372" checksum="516120AA297F5432" modified="2017-01-18" version="1">MSSSSEIAKGQKPPFANLYEVKHVAENACKACILCFKPTNTVLITGDKKDWFYVCDVHLKDKNFAKLVYCNGLGKDTEAEWRKLCGTVESLQRELRKLEKKERDKLAKEKSWLTVIPSWGAKKDESLKDEGKVKDKENGEENGSTPESEKASRSKEVVASELHDGEQKLAAFERENIKYHLEQVFYRGRLLQDFKRKKQAEIEQKLAAGTLFPTLDGLSSLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX2</accession>
    <name>A0A1E3NPX2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_23611</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CybS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019205.1">
        <property type="nucleotide sequence ID" value="XM_019162315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPX2"/>
    <dbReference type="GeneID" id="30179002"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="132" mass="14793" checksum="F624E23FBDACAA9E" modified="2017-01-18" version="1" fragment="single">LPNLRLTPDVPGNVVGSVNDAYVPPPTEFSEGSFHWAYERLVVLGMTPLVVFPFVAGVDYPLIDATLGVLIAVHSRYGFQSCIIDYIPLRKFGNWHKLAMFFLNIGTCLSVYGIYLLETEENGICDLVKRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPZ4</accession>
    <name>A0A1E3NPZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15959</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019248.1">
        <property type="nucleotide sequence ID" value="XM_019161035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPZ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPZ4"/>
    <dbReference type="GeneID" id="30177722"/>
    <dbReference type="OrthoDB" id="359154at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_uL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22682">
        <property type="entry name" value="Ribosomal_uL24m-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="41358" checksum="EC179DAE5C96FED2" modified="2017-01-18" version="1">MSSLAKINSKRYGRELTRYPHYIRKMFEQMQERSQIPAFRSESGQVDEKKRYKLPQQWTVQPGDIVMITKGKYSGTVTKVVALQNETNRVFLEHSETKRVAVPKQYWQPGQTSHIIDYPLPQHPKDLKVVGTLVEEDGTEKKIAADKLVFSGEYWDEDYKKMMPYRRVKYNENIIIPWPRPEPVQDCAYSTSGELVEERTFFPNSIVFSDSPSDLSKSMRHPLMKRPYKWGKEYLTKSDVKRLNPPSMPLSEAKLAAIEERKQIKASLPKEPLPETVSLIGDKVASFLNNITDPHYAKFINKMSPGYKDPTELFREKQKKLYDEKVRENQEMNKIKSYVEEKYKLKRSKKLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ36</accession>
    <name>A0A1E3NQ36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_26621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019275.1">
        <property type="nucleotide sequence ID" value="XM_019162454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ36"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ36"/>
    <dbReference type="GeneID" id="30179141"/>
    <dbReference type="OrthoDB" id="19619at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024621">
        <property type="entry name" value="Mba1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07961">
        <property type="entry name" value="MBA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="257" mass="29634" checksum="AF5BFD4FEE60E535" modified="2017-01-18" version="1" fragment="single">RFASTTDIITKKDPAVGIGAKSRKKQQQQRPTLNKVGVPHDPYIPVSYSHLPLSAPLKTLRALWSRVKLFCFNWVQVYQFKSGMGKGYKPQFVKWKNSAITEFVKVNKAFAERKVESVRSDVSEFVYFQLGKRQAALPKALIQWELVKFNSPPKLLSFNAFPHEDGSTLLCQIIYQFNTKQKWIIKKRGETEFTETERDMIEYLAFNIDPYTDHVVLAGSVFEAPLDRKLESKIVSSQQRALEYMNTNGDIYRTEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQA7</accession>
    <name>A0A1E3NQA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase II</fullName>
            <ecNumber evidence="3">3.5.4.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_31465</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 2 phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018836.1">
        <property type="nucleotide sequence ID" value="XM_019162702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQA7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQA7"/>
    <dbReference type="GeneID" id="30179389"/>
    <dbReference type="OrthoDB" id="5569761at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001591">
        <property type="entry name" value="PRK00393.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF29">
        <property type="entry name" value="GTP CYCLOHYDROLASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP cyclohydrolase II" evidence="9">
        <location>
            <begin position="146"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="326" mass="35712" checksum="7970B48673E672EB" modified="2017-01-18" version="1">MTHEHITQDIFETMPIISPTVSNEEQARALGLDGDGDGDGNSSLRLLPDVLPAVQCIARARIPTTQGPEIFLHLYSNDMDKKEHLAIVFGEDVRSRSLYRRRAGETQADRMIRGAYVGKLTPGRRDADRDAATGAALRFDEATGDLVVEAGTTWTQPTLVRIHSECYTGETAWSARCDCGEQFDEAGRLMGLEGHGCIVYLRQEGRGIGLGEKLKAYNLQDLGADTVQANLLLRHPADKRSFGMATAILVDLGLADIRLLTNNPDKIIAVEGKRRLVHVRERVPMVPLAWTGGTGGVSSREVEGYLRTKVEKMGHLLSKPAVRTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQI8</accession>
    <name>A0A1E3NQI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_80861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019464.1">
        <property type="nucleotide sequence ID" value="XM_019164982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQI8"/>
    <dbReference type="GeneID" id="30181669"/>
    <dbReference type="OrthoDB" id="27198at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF31">
        <property type="entry name" value="DCN1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCUN1" evidence="2">
        <location>
            <begin position="58"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="256" mass="29289" checksum="1A8EF834D3C032D4" modified="2017-01-18" version="1">MSNTDQFMQITGCMNRDLARRYIRKSGGFLDLAIDNYYNHENPAASSGTSMKQIPQRQAVEELNKVFDRYAEHANPNQMDIDGTIAYFTDLGIDPEKDVEAIIAAYTLESPSTGVFTRDAFVKNWAKVNATNAEDMSRYLHQAKHNSKLMDLVNKFAFKYALEEGKRKLAVEDAVALWRVIYNDDFAKIQEPSTMSIFINDFVASGKSGKESISMDEWNMSRLFFSIPLPELEHLSEDAAWPVLMDDFVDFLYQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQM3</accession>
    <name>A0A1E3NQM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_20948</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019517.1">
        <property type="nucleotide sequence ID" value="XM_019162177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQM3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQM3"/>
    <dbReference type="GeneID" id="30178864"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="5">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="93" mass="11025" checksum="0EE19E45A4AF4DEE" modified="2017-01-18" version="1" fragment="single">YEIREQDRWLPIANVDRVMRHSLPKNVKISKEAKECMQECVSEFISFITSQAAEKCLIEKRKTLNGEDILYSLYSLGFENYAEVLRIYLAKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQU2</accession>
    <name>A0A1E3NQU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70098</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019577.1">
        <property type="nucleotide sequence ID" value="XM_019163549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQU2"/>
    <dbReference type="GeneID" id="30180236"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033101">
        <property type="term" value="C:cellular bud membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015847">
        <property type="term" value="P:putrescine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF31">
        <property type="entry name" value="POLYAMINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="152"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="595" mass="65235" checksum="299068DF7B7EC7BE" modified="2017-01-18" version="1">MTLASDMPPAKENVNAAGAVPGTAITATAVTNAAEPSEGSSSSSHDDDEVPAPLATSDVEKFDGDLERGSMESDESRLMRIETSSNLSRELSRKLTGADNLKVDTSTPIPRMGKDRDYPPPLPDSSMYSVAFDGDDDPLHPHNWPVMKKLIQCSIVGANTFCIAFGSAIFAQAVLPLSKIYHVHYVVTTLSVTLYVLGFATGPVVWAPLSELYGRRPVLVMSSLAFCCFNFAVAVSDRLESILICRFFAGCLGAAPLVVVPASFADMFGNETRGTAIVIFSMCVFVAPLMAPFIGGFIVKNPSLGWRWTEFITGILSAASFVLIIFFETETHHPIILVQKAREIRRRTGNWGIHAPHDEFSLNLSEIVENNLARPIKMLFQEPILFFITLYNAFIYGMLYLFLSAYPIVFAEGYGMSGGVAELPYFGLVIGQTLGGLFCIWSERAYVRKLKENGGKIIPENRLPPMLVGGICFPIGLLWFCWTGNYHETVHWMAPTASGIFTGFGLIAIFNPSMNYIIDAYLVFAASAMAANSFLRSAFGAVFPLFATFMFHNMGTNWAGLLLGLFAVVLILCPLAFMKWGKKLRQRSKYAFDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQX1</accession>
    <name>A0A1E3NQX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_56715</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019565.1">
        <property type="nucleotide sequence ID" value="XM_019163195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NQX1"/>
    <dbReference type="GeneID" id="30179882"/>
    <dbReference type="OrthoDB" id="268428at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="193" mass="21661" checksum="67E116C8CA3A3321" modified="2017-01-18" version="1">MDIVLGIRLKDCTLIATSKAFTRGISVLKATDDKTIDLNTHNMIAFTGESGDTTQFAEHIKANVQLFGLREGYDMESSEIASFVRHELATSIRSRKPYQVQILVAGVKDGGDGFLSMIDYLGTRCDLPYAAHGYAAFYTMSLLDHHYKDDIDLDTGLRLLKLCEEELNKRMPIDFKGLNVKIVDKNGIRFIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRM6</accession>
    <name>A0A1E3NRM6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_9248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019834.1">
        <property type="nucleotide sequence ID" value="XM_019165079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRM6"/>
    <dbReference type="GeneID" id="30181766"/>
    <dbReference type="OrthoDB" id="3995392at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="159" mass="17427" checksum="2C671C961C9B43D6" modified="2017-01-18" version="1">MAVSRSDSVSSFSTAGSSQFVDANSKFADAESEMQKQPTDSLSHTEDQTKVQSIADPEKEQDTDAVAEQAEVKTKQPEPVKEPKAYVSKQEKIRQLREQNPQKGYQREEGECHEYVCSNNILDVLDLPNWKGLCGSLGVMCCNSCCSSGTSSVQKAAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRP8</accession>
    <name>A0A1E3NRP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14288</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019873.1">
        <property type="nucleotide sequence ID" value="XM_019160310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRP8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRP8"/>
    <dbReference type="GeneID" id="30176997"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF25">
        <property type="entry name" value="F-BAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="244"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="557"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="752"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="812"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="866"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="916"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="816"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="872"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="916"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="972"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="996"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1057" mass="116001" checksum="9E72C6F993B897AA" modified="2017-01-18" version="1">MSPSRNSISNWLKLLKKSDSSNDVTSPSNSTFNPSNLDLSEPSGAAANTSAAEHHGSDPPASNLAGAPAPANSRLYAPTPTKRTNSGSSPRKNSNPLSLNLLSPQKSGTPQPLSSSPTPSRPFLGFKSRSSHSFRPLSQFLDPSSSSHQDHHSSSGAASGTASDTPDDPNGRLRSHRDSFLQQRQLDVIGNSSIFGCDIESSTKLAHGTVYISADVPANTADDTTTTTTDSDANTRTSTSPSTKHLNVAYGQVPLVIVSCGSYLKANALNVEGVFRLSGSNKRIKHLQMIFSTPPDYGAKIDWDGYTVHDAASLLRRYLGSLSEPLIPFNLYNSFRDPLLEKKELFLYLKEKDSKGALQVKPDKQKRRVIVAQRRRLLKEYALLFQKLPPIQHRVLFYLVDMLAMFNLKSDKNRMPAKNLAAIFQPSILFHLDHDMNPEEYAANSVVIEFMITYSHKILVIVQREKPSPPLESTPAQAKNEAISSSRIPTDNYEKIVEEADELDDLDEMDECGEAHADTKASNEQHDENVKVSNENYHPITSDAFTHIKPAITVEIPAERTSPNSKTQSQILYHPNDSRSSPLRGSPLRQVFTPVNDNHDISGLENLGAPVTLFSSPRVTRSRPHSKSLSHVPHSEVVRIPASCSTSSNFKALGSTDDNLSRIMSNQSGVSSLLSSRLGSETAEGTTNRIDNALAPADGNNLINELGLHQPDAIHEGQVVFIDNSSEVSCLAKPPGLQNPSDCPDYDHKIHSFIPPISPTTATMSRDDTPDVENMSKNFQNNSKNLTQSETSHSNNTGVNSIDEYYSLHDEEKRSNMSTASDYDTDSMIGTNEDTGKSSNSLAFSFQPSLLQGHKSNDDMHLLNTRARDNSRANSKNLDDSSQNQRETSPFLDYKVVQYSHPDIVNDPSLLRKTATTPSFGSTNASLNDESGVISSKKRSNSINASEDKGTGNSGTNKQSGIFSIVATHMPNSNTNRNVVSSSEKASHSSGISKSNLREKGKGKKETIEKGKDVKSKERSKDREEENFKDKEKIKDKTKEKRTWFDKLKNRSNAKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRQ0</accession>
    <name>A0A1E3NRQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019346.1">
        <property type="nucleotide sequence ID" value="XM_019164123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRQ0"/>
    <dbReference type="GeneID" id="30180810"/>
    <dbReference type="OrthoDB" id="3997944at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018857">
        <property type="entry name" value="TORC1_cplx_su_TCO89"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10452">
        <property type="entry name" value="TCO89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="682"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="722"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="751"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="776" mass="87069" checksum="1FE184715EAA3351" modified="2017-01-18" version="1">MTEMIKEGSTENIGSSVPLPKRKQGKQFTVNGSKKSSRISSFGNLKNAAKLTRIRSVDSMGSNPGGNESERPRIARTRSNVDLIRSVSSGPNIYSRPNKAKSMTNLHLRKARKGKSSADAEFDAQSDQNSDDEQKSMSNKDNEDYEDYEENEDEDDYYVDRRTGSIATDEKKANGEGVSSNTNTSNEENTQVGARDQILEQLKENVAEMKIDSSPHDAADKQFGQESKTSDEDISEGQKNILDQSDDFKEGENSLNMYRMHSVLSQSTGQERQLSNNHVASRQQSSNTEENPQNMEKEESGENASSHNDSNTTVSSSKSGMPYHTNQDSLIFQQSGLTLYNLNTQPSVKNLQLSQLNATNMKATASHPSLQRQESYLSFGVPENLQHRLQMSTIQGNTQHPQKVKSSSPSENSKSRASAEDKEPPEDLNNNKASFEEKTPQNVSLLSKQARSMEYGGNSDNQQSEILDLDLSSYLSSQEPEIESRTQQKLWLQRENVATLNDFEEGTSQTPSQIINQTTRFQYEQLSREFLHIRRHTNPMLGSLQRIYGTCRHESHPSGKKLDVPEPVKTVSKSISKSSLRNSRPATANSMSKSLGLSLKEAELTGGPFTEDLESYNDELLTLWKKSCLHFKEPQAPSLRSGANSSNSFNQGGVALSAKYGYQPNTSGQQFSQAFARGNHTSFGNTNLHSNTSGNFNGFGNQVNHFNGLNRSSQYQPTTRAQQQQQQQQQQQQQQQQQQQQLEQQRRHQLQQRQQQQPPLQQQNLHHQLQQSHQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRQ7</accession>
    <name>A0A1E3NRQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Up-frameshift suppressor 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_14336</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019920.1">
        <property type="nucleotide sequence ID" value="XM_019160330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRQ7"/>
    <dbReference type="GeneID" id="30177017"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Up-frameshift suppressor 2 C-terminal" evidence="4">
        <location>
            <begin position="1037"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="807"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="812"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="840"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="868"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="928"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1006"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1031"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1177" mass="135334" checksum="ACF7551F86518862" modified="2017-01-18" version="1">MSSNDEFEKRRNELYKQNMKAWNGDLFETDGNLDTSLKKNTAFIKKLRQGITKENESQLVQGVRTISLEKYLNEISSALCEALCKVSKTTDIQCAVEVVSALHQRFAHTFTPFVELFFAYSMVQPSTKLLKKDEKDRQVKIKNLIRLAMEFFLCGIFRSIQDMPLTELPLYLTRLKTSSKDDIPVIVPMLKEIMSYDFVSGMSLPGILSFLKRFSSLIYVKGPRYSENYMIFVRKLLKSYIKNAVSTTETYHKSIVNKKMTAQVVSLKTGKIAASIEGKIEELQHQFEEFNSFCEFSCPLADISLPALESEIERTENSVVTVQHSVSKQNDIWENEEARRFYEDIPSLESIVSTEVLESINAKILPHEMKGSKSADIILRLDNADLAEDVDNVVKDFWEWGLSNKASRNRLSKHIVELRDTTKFRNFARFLKINNAYFEKCIEDLIARLDKSFRFQMHHDSITEKDILLFAELVKFKLIPVHMIFHKVRTLILNIEVNNNIDLLSLMFDACGKVLVNDDEYKDYTKGLIQLLVQVYKTKQFSHTDKYAVKVFLLSLNPPKLKEQNIVALSKEERFLLYIIKTQLSEKTYELVAKRFKNSDWDNPVIYRTIIQILSKPSNISFVNIEFLARVFRLLCVPSRYTLRTEVVDQLLENITTGLEENDLQQSRIRLSQGVYFIHVINLRIIEEEYLFPILYKIVCFGHQGYNPEREKICELDRPDDHFRIRLICSMLLVLDVKRLRLETIIGLETFYKFFNYYVFTKSGPLAVDLNSKLDDTSMYLKSCLNSENFFRPNSYEDSLTMLQEAISKSKSTSETESKELSKNGEVKGDAEGSSSSVVQKKDPTQKKEKCAEHFDDDEKLGRHNLNDDDDEADDDYADDDEDDDEGDEDDEDDDDDDADDDDDDGDADNRADVYRGQAKSQIKSEGLDSEEDTDVEEDIDDIGGNYYDEDDDEEEGDDDDGDDDDDDEEEEEEEEEEEEEDDDDDDDYDDDYDEVDVSACGYDTDSYDSEEDSESDDNSANGNIEVVSDFVDEEEEDEDDMTNREKAFFDAELDIEFAKIMNESLTNAAAAKPNKGFLSDSMGTPESIIYKLKKATTEETGALATADHDILRPTHSQKDGSVKFTFLSRSGKKVQQKDISVPDSSILAINVTREQERMRTEKNKIKSFVLNSVHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRY7</accession>
    <name>A0A1E3NRY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019571.1">
        <property type="nucleotide sequence ID" value="XM_019163545.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRY7"/>
    <dbReference type="GeneID" id="30180232"/>
    <dbReference type="OrthoDB" id="3995715at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="692"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="816"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1155"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="582"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="631"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="837"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="869"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="894"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="929"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1316" mass="146887" checksum="0672A95EBA0EF5A4" modified="2017-01-18" version="1">MAWNKRTLVYVEPRVDPARVLQDVQRVDRGRYVVKTSAETAGYVARQVKYSVEDFHGVVRDGELAALLDTVGVLQLTVRRAGEDAGDAAGGYFNEWHAWMRPGTSVNVAPQHVDTWRAYRDVSETLRDALVGLLRGGAGGAGGDGAALTEMVFEREWWTWSALDGGPGTADGLARLLQTLGLDDGDVRRADYDAAELVLDKTRLSLRFALDYDPWSLLPHDEHISDTSACRLHADLALSREYILDRYELDRLAASTHSCLAGLASISNGGMDLELPAHRVPDHRYSVSSVASANSASHHQLDHIIPRSSSVSEMLDIDDKFVRKPKAKPVYNKMNRNSRNYAAADGPTASTPQLAHHRQQSRNQGQNTRSQRSHSQHRQQPYQQHPQYQQYQQYQQYQQYQQYPQRQQQQHYPNGYPQNSQQYQQYQQYQQYQQYQQYQQYQQTRPQKQQQQQQQYPQAQKQPLQSQPYPEYQYTQHQQSMPQSYQKRNTVSRFDPTLKQHAHSKSQPPSQQHHQQQLRPHQPSKSSSGFYNNPQAPSSNLNIPPQALGSASNLAMRPANRSFYPPVVRPHTTPTSFTSYHSNTTGTASNPNSNSAPNSGANSNQNSNPNSNSNSNPTLNLNTLEIPNAVTSSSNTNTNTNSSRGQTPGKSSPSTTNTTTSESSSSTNRTPVKQSVSNSKINIVSPLTDISSAEGLDDTSVNSQTPRTLKNPTQHHGQQHQFQQQQLNVGYNHAAIAAKAAVRNVPTSAPAPSIRPIPVQTSAYRTPATTDIPNLTPVPRATQVYEDNPSSVQSSLRSEQKRGFFKRIFSRSKSIKDLSPVDRSPVSSPKDSKFSLRRLSLIRSSSRTSTDRSQSTSRASSIDARGTKRLSTSSRSSLKVNKDLAADVNCNKGDASSSDIPDYKNSTPSNTNSTRIFSPVTEATEADSSHNTLSNTDLPSSNRTISDFNFGETENYNHMTDLKTPLSKNVESAKHSLVGVKSNNSSFFSANEGGKQASNRIIQERDDTHQLQTVKTTETSDFSETDEFDTSMASISKIDSSVAKKQGCDNVVLELGYLSTIVGLGETSFFNSELKRTNTNKSTTTNATTSTGKSNTEKDEHGVMKLSVPTNDTKNVVKKSSWGSIRAKDERKKMVRKLGRKIPFARLRERFDISFDDDDTNSHHTGAESDSESTSYVNKSILEDDDTDFELHSGLDYDSEFFQNLKTKHILPANHEPRHGEIAAGSLKGCMKEGEEESHLFHPKSVDVGFIDDAQYGVTFSNEVYDRSNPDMKKVYVMMTYYPREIRAIRIELNDFKKNEMVVNEASKQYTHTFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS12</accession>
    <name>A0A1E3NS12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CAF1B/HIR1 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_38842</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020008.1">
        <property type="nucleotide sequence ID" value="XM_019163107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS12"/>
    <dbReference type="STRING" id="763406.A0A1E3NS12"/>
    <dbReference type="GeneID" id="30179794"/>
    <dbReference type="OrthoDB" id="71227at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="10">
        <location>
            <begin position="18"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="10">
        <location>
            <begin position="282"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="460" mass="50524" checksum="59E32AD480DDBDAA" modified="2017-01-18" version="1" fragment="single">MSNPGEARIQHVRAKTLTLHWHEGAAAIYSVGTQPGKPGERSARLVTGGGDNNVRIWKVNYNEEGSDVVGVEILSSITKHTQAVNCVRFNSTGDLLATASDDGTIMIWEMSDTVVQEFGQDVDDDIKESWVLKVACFASNMSEIYDICWSPDSKYICCGLMDNIIRIFSVVTGSMVKQIAEHSHYVQGVAWDPRGDYICSQSADRSVHVYKVSTNESGELVVGPTAFYKSVKSEVPALKFSQLTSENLSKSSAVISSQEKSSSNVFNSNSGDVFSAPASNIPPISYKQHYLYHNETLQSFFRRLTFSPDGSILVTTCGIYKPNAENGKDEIQNTVYVYTRAGLNKGPVVHLPGLRKPAIVVRFSPVRYALFETSSNRQPNKPEGADVNVFSLPYRMLFAIATQDSILIYDTQRLKCVVVVTSIHYAVITDVAWSSDGRTVFVSSNDGFISCVVLGEDIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSN6</accession>
    <name>A0A1E3NSN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_96304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020280.1">
        <property type="nucleotide sequence ID" value="XM_019165117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSN6"/>
    <dbReference type="GeneID" id="30181804"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="116" mass="12775" checksum="0CC47824DEA7FC53" modified="2017-01-18" version="1">MTLRMLCLPRKGTVVVACRQFRWRAPPLPPTAGWSLLAKNTPCGRRERQRTCGITPARGSSGCFLTHFLQLIRGTRAGLLLLPCLPFAVAFVFAVLEIFGFSSTIQTSGDRCSLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSV0</accession>
    <name>A0A1E3NSV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA damage checkpoint protein LCD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70362</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019861.1">
        <property type="nucleotide sequence ID" value="XM_019163619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSV0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSV0"/>
    <dbReference type="GeneID" id="30180306"/>
    <dbReference type="OrthoDB" id="4078000at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018622">
        <property type="entry name" value="DNA_damage_chkpnt_Lcd1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09798">
        <property type="entry name" value="LCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="767" mass="88725" checksum="9BFBEFB0119A6D49" modified="2017-01-18" version="1">MPLENDDTFDDDDDFDLEQVYNNAQATQPSGQIQTYGPKDDIYQMKGENAILRGQIEKIHKEQTEAQKRLKEDFDKMLRDKESYIQALNESLSKMKSENEFLVSENKNLYQTNSRKRKLRSVDSNSTALSQHETSTQNTSHMSASTQNTTPAMNEENNSRSEVERISEKKSHTDIKVVVVNQATFFQDEKTLFIEAVTNYVISGMPRPTLNYLENISSTFDYTSNDFQILANENSFKSAILRYLINFQDKNRIDYLLINFIEILLEYVIQTFELDESQLLPIPFLLSLINFALNYRPKAINEKFIGETTDKIVSMLWHFQGIFKPEFDYLSLPGSNHMMNFVTMQDKENSGLMDYSFVEKTMHVKILEVFTAIFLMDLLSTLSKVASFHVFTFTNSSANSTFWKKVPQQNMVNSFLSRKTPIHFIYSTVEILLNSIHDDDRFAFGNTRRNSITKPTTANEITIQILEQIMQFLTTISPSQIHFNVFGLNHLIGSNHHLELLEMVSIPSNKLSSYPQTHSFDQYEEILRTKFEYFQKQEHYILSTKMLIMDLFESFYSALIMITLPLNTNSKLTRILCQMIGEEQEMIIRSPRSSNNNLRIDLISKSVKILHHLIAQESSVKINELSNLTLREMIIVLLRISSKSMKNESIDFVTKLRHANYNGVLFNGVQEKNELDKYGLWNSILSLTKLPPKEQKRVTENRIQIEIDLYNGIEFGYSDETIDLARDIVGMCVTGDEADRLHDSINYVNDEDEDYEMAELGDFEGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT33</accession>
    <name>A0A1E3NT33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxisomal ATPase PEX6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Peroxin-6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_70427</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019941.1">
        <property type="nucleotide sequence ID" value="XM_019163639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT33"/>
    <dbReference type="STRING" id="763406.A0A1E3NT33"/>
    <dbReference type="GeneID" id="30180326"/>
    <dbReference type="OrthoDB" id="5553750at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904949">
        <property type="term" value="C:ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19527">
        <property type="entry name" value="RecA-like_PEX6_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000109">
        <property type="entry name" value="Peroxisomal biogenesis factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000039">
        <property type="entry name" value="peroxisome biogenesis factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056995">
        <property type="entry name" value="PEX6_4th_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047533">
        <property type="entry name" value="RecA-like_PEX6_r2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF9">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23315">
        <property type="entry name" value="PEX6_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="525"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="822"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1012"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1094" mass="122223" checksum="E4A43382802AFAEF" modified="2017-01-18" version="1">MNVSVSLSVEARNSLQFVLLSKDIFTSLFGPGADVRRKYILLTLHGSNVLDTPQLYPITIKHELKDNHVEIESLKLLQQHGSDFTIETATVRKIDRVPKLTSVVVSFPEEVYSLLKYKERDQIHELLAQERESTLDFAIVRKGDTSHTLNGSFIHAEPFEQGYIDHTTTITIVKENKTLGNSTVDLSSDGADIEEFDPFDINSTISPTLTDSKPTSHAAQFNVRPLELDFLQKYTHFSKYSDEFPFEDEQLFACLTFSAFQKLHCLSGDLLLLQTAGDGKNGFGPTYVRVFPFIEPCSFDTQSIYLSPLLILALSNPESVSVGSIPNQSHQRADLPRKKLTKFIPLAKELILSRVSTPTTSDRSLQYLFLSNLKAYFESSNRVITKGQIIPIALDSSLAESVHHTYGLKGWYPEVVPEGNPDCIAWFMVTNGTFKDLTTGEIKPIESGKQYLVNSNKTRMLQSGISKRDLSDVDFPTITRYLNIKPKFEYPNTILPGGGKYTFPYAKTLRKIFDTSIKIKGKIALQTTVLLTSNTRFVGKSTLVETVCEDFGFSLVELDGYDLVNSGLGMPAATVGILKGKLDRLVESCERLAVFIKHIECLCKKGDEQQADQKSLDDSLSLNIVTLLNEYALKGVIIITSTNDADSINDIVRSKIKFEIDVNVPSEPERKEIFKYLVNFPTSSNEFIYKHNDEYLYLASNDVSIDTLALQSAALTPNDLAYIVKNVKLNALRRVKKECKEVGLKIQDIIDINSGAIRLTPVDFEVSINKARNKFSDSIGAPRIPNVKWEDVGGLDVVKNEILDTIEMPLKHPELFGSGMKKRSGLLFYGPPGTGKTLLAKAIATNFSLNFFSVKGPELLNMYIGESEANVRRVFEKARDAKPCVIFFDELDSVAPRRGNQGDSGGVMDRIVSQLLAELDGMSGSDGGDGVFVVGASNRPDLLDEALLRPGRFDKMLYLGISDTHDKQAKIIAALTRKFNLHPEIDLQLIAQSCPFNFTGADFYALCSDAMLNAMIRTAREADLKLEQYNQTMEEKDKLNIRQWFEKVATDKDLEVVVGTEDFHEARKELVASVSEQELAHYLRVRENFEGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT41</accession>
    <name>A0A1E3NT41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCA7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_75847</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020384.1">
        <property type="nucleotide sequence ID" value="XM_019164942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT41"/>
    <dbReference type="STRING" id="763406.A0A1E3NT41"/>
    <dbReference type="GeneID" id="30181629"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904802">
        <property type="term" value="P:RITS complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037804">
        <property type="entry name" value="SGF73"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041251">
        <property type="entry name" value="Znf_C2H2_13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805:SF1">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18508">
        <property type="entry name" value="zf_C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="2">
        <location>
            <begin position="215"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="489" mass="53977" checksum="E1242214671044E4" modified="2017-01-18" version="1">MSKYASPDLIAKAIGVNAHSVINNIKDTKDDKLGDSTKINDRQNTGEITELDEIPELRHWRDLGSYLDTLKNVPEKNTTDSGVFESSSRPLTQPINYRICNSCGTAILEKNMADHISHCSTDKIKKEPTNEKTEVAETIITADNADSSTSKKEVAVKENSSPKKISKPTKKIVKKRKNEPVEDNTEVSNKENTAEEPAQKKLKTERKKKEPKPKANKNKGPVDVERQCGVPLPNGGFCARSLTCKTHSMGAKRSVLGRSAPYDQLLAAYQRKNQAKIGAAAAAAQQAKDDLMHGAGVALDDEEETQQVLSGVCRSSAMPLERNVCIPVRMRTGYLRMREMFASSLLPRLQNNPLGKMYARAAVMNVENIGENSFFPVRSQHQKNNQQRQNLKHNQNAVHLQVQKQTQLHAKVQAQAQAQAQAQAHAQTQAASMVSQQQSSGKKPVSNDTGMAGNYNVSTRGMNISTLTPQQQQQMLMLGQVQMMKSLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT71</accession>
    <name>A0A1E3NT71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020385.1">
        <property type="nucleotide sequence ID" value="XM_019160506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT71"/>
    <dbReference type="GeneID" id="30177193"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="81" mass="9063" checksum="2119F6E044A08D11" modified="2017-01-18" version="1">MSQKTVGGRFNVMSFGLIGGFLIGLYLVKDYQIIKYSPPNPENFDENGNWKESSFIKVRLSDKVAPLEKKTSTEGSSSEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT89</accession>
    <name>A0A1E3NT89_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S acidic ribosomal protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_14821</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020468.1">
        <property type="nucleotide sequence ID" value="XM_019160548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT89"/>
    <dbReference type="STRING" id="763406.A0A1E3NT89"/>
    <dbReference type="GeneID" id="30177235"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="10925" checksum="CC16E55B3C5F6BD2" modified="2017-01-18" version="1">MSAATETALAYAAIILADSDLEVSTENLNKLVAGANIEIDGIWGQIFAKALEGKDLSSLLVNFNVSSAPAAGAVAGASGAAGAEEAAAEEEEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTL3</accession>
    <name>A0A1E3NTL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020016.1">
        <property type="nucleotide sequence ID" value="XM_019163657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTL3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTL3"/>
    <dbReference type="GeneID" id="30180344"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF29">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="63248" checksum="633B4B9E694AC84E" modified="2017-01-18" version="1">MSASPNIEHMGSRTTILSVDDMKITSVLSGQHERIIVDHDVDHDEAVILALGYKQEFKREFSLFTTFGVSFSVLGLLPSIASTLWYSLAYAGNAGITWGYLIGMVGVMGVACSMAEISSAFPTSGGLYYATAMMAPPRYRAILSWIVGWSNYFVQVTGAPSVAYGGAAMILALKQVVDDTYVYHTWHCYLLTVGLTFFAAIIASAPTKWIAWINSCSTILNLIFLFVSWIVILGGNSRQDQGLPKFNSNKIAWGLDNLTDWPDGICILMSFMAVIWTMSGFDSPFHLAEECSNAQVATPRAIVLTASVGGILGFVFQLAMAYTIVDVDAAVTDSLSQPYISFLIQVLDRPSALCLASFAIILAFTMTFSCMIAASRVLFSYSRDNCFPLSRYWATVNKTTLTPVNAVWMNWFIGCLLCLLMFGGVAIDAIFSVGAIGSFISFTIPTLLRITYARSFFKPGPWNLGKFTYLSGSLAVSFTTLMIPVLCFPQYRGANNSPSSMNWTVLVYWGSLFLAIAWYYIYAHKIYSGPRSNLDKGSVIKDGEEDDVIQTIVSKGGKIKYGASEVTDEDQVPIEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTP7</accession>
    <name>A0A1E3NTP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_14869</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020523.1">
        <property type="nucleotide sequence ID" value="XM_019160575.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NTP7"/>
    <dbReference type="GeneID" id="30177262"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="493"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="639"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1193"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1232"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1274"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1304"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1335"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1429"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="132"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="818"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1474"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1474"/>
            <end position="1491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1498" mass="169519" checksum="D055DDD2FC8CCCBD" modified="2017-01-18" version="1">MEKPAENHSNDTDSIDSDNQDVQKFNPLNDPEDQGNAENLSHHVSRILSTQEGMERVATLARVLSTKTKKDMDHFEINKENFDLELLLKYLRDRSAEQGIESANAGVSFRDLTCWGIDASAAHGPSVTEMTLNYLSFWKMFQKDNRKQRMIIQNFVGVLEPGEMVLCLGKPGAGCSSLLKAVAGEIENFTKVEGSFSYDGLDQAEMMEKYKGYVVYNPELDFHFPYITVKETIQLALRCKTPEKRIDNLSRAEYVDNMLKLWCTVFGLTHTYATRVGNDFVRGVSGGERKRVSIVEALALNASVYCFDNATRGLDASTALEFTQCLRTATNMLGCSAFVAIYQAGQNIYELFDKVSVLYKGRQIYWGPADQGVSYFEKMGYLKPSRMTAPEFLTAVTSPSSRQVQPGFEGKVPESSEEFADYWVNSPEYATLMTEMDEFDANHNGDETRQRLDFVNQQRKQKGNRQKSQFMVTYWSQLYYLMLRGFQRTKGNITYTIVYVASFVTKGFVVGSMYYHIPKSTNGAYSRGGILFYVLLFCSVTSLAEIANSFSTRNILVKQKSYSMYHLSAEALQEIITEFPTKLIAVVILALTTYWMPNLRHTAGGFFMYLLFLLTIQQCMSFTFKCVATVTKDGTTAHAIGGLWVLMMCVYAGFVLPLTQMHHWIKWIHYLNPMFYCFYSLMGNEFHRRHMDCYQLVPSGPGYENTSLSNQICSIPGSIAGRDWVLGDDYLYKAFDIKWHNVWKYWGINLCWTVGYIGLNVFMSEYIKNVEGGGDLLLFKRGCLPDTSAGEDWDGKVATRGEMMLALNGPDADLEEIIATADIFSWKNLDYIIPYDGATRQLLCSIQGYVKPGTMTALMGESGAGKTTLLNVLAQRISFGTITGDMLVNGRPLDTSFKRRTGYVQQQDLHMAEYSVRESLRFAADLRQPKDVSTAEKYEYVEKIIGCLGMDKYAEAMVGKIGRGLNVEQRKKLSIATELVAKPSLLLFLDEPTSGLDSESSWSIVQFLRALADSGQAILCTIHQPSATLFEVFDRLLLLKKGGKTVYFGDIGANSSTLLKYFEGHSGVKCGASENPAEYILNCIGAGATASAAQDWAELWNNSPECAQTTKDIEYLHAELPKRPENSTAGDLSAKYATTYDKQFWYVLRRTYVQFWRSPVYIRAKFLECVSCALFVGLSYVGMDHSVGGAQGAFSSVFMLLLISVAMINQSHVFAYDTRELFEVREALSNTFHWSCLLLCHTILEIFWSTICQFFIYVCYYWPPRYSGDANKAGFFFFINVLIFPAYYCTYGLAILYFSPDVPSASMINSNLFAAMLLFCGILNPKRYSPRFWSFMYVASPFTYFVQAFVGPILHNRKLVCAYGELSIVDPPEGQNCGDYFSHYIDNNGGYLRNPDADSSCQYCPYTMQEQVVIQYGIKWNQHWRDFGIAWIYIIANTGFMLVGYYYFRVIGKNPFGMIFKLLNPKSLIPKQRHEKDNTIFQKKPGDDKIGTQKKPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUH0</accession>
    <name>A0A1E3NUH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_25598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020336.1">
        <property type="nucleotide sequence ID" value="XM_019162404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUH0"/>
    <dbReference type="STRING" id="763406.A0A1E3NUH0"/>
    <dbReference type="GeneID" id="30179091"/>
    <dbReference type="OrthoDB" id="10266249at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2030">
        <property type="entry name" value="DNA/RNA-binding protein Kin17, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056767">
        <property type="entry name" value="C2H2-Znf_KIN17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019447">
        <property type="entry name" value="DNA/RNA-bd_Kin17_WH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037321">
        <property type="entry name" value="KIN17-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038254">
        <property type="entry name" value="KIN17_WH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12805:SF0">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12805">
        <property type="entry name" value="KIN17 KIN, ANTIGENIC DETERMINANT OF RECA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25095">
        <property type="entry name" value="C2H2-zf_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10357">
        <property type="entry name" value="WH_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01253">
        <property type="entry name" value="Kin17_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="131" mass="15009" checksum="87DFDDA763AD27A8" modified="2017-01-18" version="1" fragment="single">KYQEKQRRRRGLQKTRFYCQVCARQCLDENGFSCHVKSDVHMRNLRKQLAGNEPGGGGATAAAALIARYSDQFAAAFLALLRRTHGDKPVALNRFYQEYIRDRDHVHLNSTRWRSVTAAARHLHAAGRCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUI0</accession>
    <name>A0A1E3NUI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_57305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020346.1">
        <property type="nucleotide sequence ID" value="XM_019163229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NUI0"/>
    <dbReference type="GeneID" id="30179916"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018808">
        <property type="entry name" value="Muniscin_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10291">
        <property type="entry name" value="muHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD" evidence="2">
        <location>
            <begin position="669"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="943" mass="103555" checksum="3EA426DFA323D3EF" modified="2017-01-18" version="1">MSSSEPAYGVNGTADEQRVVYSSSILFEKQPLEAMEALMIRRQQAKLINKELAEWFTAYGRLRLHYVEELKKIHKRGQTLFTDSSPALQNSKLDALGLCNPLWSDVLNILDDEINLFDQSTRKMGRDMIAPLRLFTRNNDSNLAEMDELSKLASNLQNPEGTSPEIYEDEWARRAPYFFEIFENYDYQRLLLLKDIFLKYQTDVGDVVGKFKKDNENGLEHALNFSVDDEIKRFSQDVIATKLPIENIKVAKADKSRTSTDGFTTSPAAATEPSSTFSSKRHSGLLSPRRFHIKKNGGEHESESSAPSSNLNPDSDQASSSNISKRHTSNTSMLSSNTAAAANNKKEKNSMRSKFGSIFKSHKKGGRDSMYVPPSAIHEPDNASINTETTTGSTRARTQRQRNQSVASNATPSQASVRQYRQQEDQAYKSEETPSREATPVKQSQHPYRQSTNYSNNNLSNTNINSSAETKPLPQYPLAQKSRQSFQPPPPPPQQQQQQQRPVVQERPTEASLFEPMRPTKRSDSLSSSNVPQVGGLASASTGTSESVATPPITSTSAMTSIPPVSREMENIPESSPLVASPPPPPPPSSRKHLSTDMSMLNAPPAARQPHGSVPVPPSQRRSVSNDQILQPAATGTHQPDNTSNLLAKNTTGGGSLMGGQIMHPSLTTPGLNSSIVELFNATFKDGKLARSNAIGEVAFSFIVDETNKKLPNEIGLQISSKSNGLLPNFMVNPMFLQQSATSPGSFSISDPTQISMRTVGGLKYMLNNPTPPIVITPIWKHEDKQSTVIISIKPTSELESYLAHESLSLSNIMVSVSIQNAVVLSAATKPPGSLNKEKGRVTWILPHPIVFNFSKKEERLVARFITNSRASESDTGVQMRFNISNEDGAGRIDFLNTDMEIKASGVSMVEDPFGDGISKQIDDWSEVPTLKSLVAGSYSGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUI9</accession>
    <name>A0A1E3NUI9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <ecNumber evidence="4">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_57312</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020356.1">
        <property type="nucleotide sequence ID" value="XM_019163230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUI9"/>
    <dbReference type="STRING" id="763406.A0A1E3NUI9"/>
    <dbReference type="GeneID" id="30179917"/>
    <dbReference type="OrthoDB" id="60371at2759"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000002">
        <property type="entry name" value="3,4-dihydroxy-2-butanone 4-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF18">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="383"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ49243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="433" mass="48529" checksum="E2C15CCDB99684EF" modified="2017-01-18" version="1">MSSAEEVKFTSIPDALEAFKNGEFLIVMDDEDRENEGDLIIAAEKVDTAKMAFLVKHSSGYVCVPLSTERADELELPLMIPEESMTDRHGTAYTVTVDYEDGTTTGISAHDRALTTRKLAEKSTKSTDLLRPGHICPLRAKPKLLRERTGHTEAAVHLCVLTQQQPAAAICELVREEDGLMMRLSDCWKFSKKFDVKIITIKHLIDATMNRLFGTKSKVPKATLDDAMGNIDNRVSSLDVKITKVNSELATYQQKLSKMREGPGKSAIKQRAIKLLRQRKQLETQRDQLMSQSWNISQAQMTTENLKNTMITVDAMRQTNKELRKTYGKINIDKLEDMQDEMLDLIDQSNELQASLGRSYDVPDDISESELDAELEALGDELDINEEMGGEQLPSYLTDNGDSEYKLPSFVEDEAPKSEEPESKMPAQLSNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV57</accession>
    <name>A0A1E3NV57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020661.1">
        <property type="nucleotide sequence ID" value="XM_019160621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV57"/>
    <dbReference type="STRING" id="763406.A0A1E3NV57"/>
    <dbReference type="GeneID" id="30177308"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043254">
        <property type="term" value="P:regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01862">
        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000086">
        <property type="entry name" value="Ras-related small GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="207" mass="23018" checksum="8D016F2FE920E0C8" modified="2017-01-18" version="1">MSGGHRKTLLKVIILGDTGVGKTSLMQQFINGKFSQQYKATIGADFLPKDMSIDDKNVTMQIWDTAGQERFQSLGVAFYRGSDCCVLVYDVTNPKSFENLQSWRDEFLIQANIKDPDNFPFVVIGNKIDVEESKRLVSSKKAQSLCASLGNLPYFETSAKEAVNVDQAFDVVARSALQQEESMEFTDDYTDAVNIQVDGDNYSTCGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NCZ1</accession>
    <name>A0A1E3NCZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_60718</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015165.1">
        <property type="nucleotide sequence ID" value="XM_019163455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NCZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NCZ1"/>
    <dbReference type="GeneID" id="30180142"/>
    <dbReference type="OrthoDB" id="425114at2759"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000041">
        <property type="entry name" value="Adenosylmethionine-8-amino-7-oxononanoate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004624">
        <property type="entry name" value="PRK05964.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005940">
        <property type="entry name" value="PRK07986.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684:SF17">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="436" mass="48661" checksum="1DE93816B3535968" modified="2017-01-18" version="1">MQKVESLLKYDREHVWHPYTSTINPLPCYLVDSAHGVKINLATGETLIDGMSSWWAAIHGYNHPELNEAAVCQLQKMSHIMFGGLTHQPAIELVKTLIDMTPPELEHCFLADSGSVAVEVSMKMAFQYCDTRFGLDEACQKKKFMTVNYGYHGDTFGAMSVCDPVNSMHSLYSGYVRENIFVKPPKCRFGDAWDEKYIASFAKAIEKHHHEVIAVIIEPIVQGAGGMRIYHPEYLKQIRQLCTKYEVLLILDEIATGFGRTGKLFAYEHADITPDILCVGKALTGGYLTLSAVLCTKEVAGLVCSGRAKGFMHGPTFMGNPLACSIALKSLELIKRGSWKTQVQAIESSFKSALPALRDLDIVRDVRIIGAIGVIELTVMVDAKWFQEQFVQRGVWVRPFGKLVYMMPPFIINDSELGKLIKSVVDVIRLFASIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDD5</accession>
    <name>A0A1E3NDD5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_79882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015248.1">
        <property type="nucleotide sequence ID" value="XM_019164975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDD5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDD5"/>
    <dbReference type="GeneID" id="30181662"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="30"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="537" mass="59978" checksum="012AAEC0EADC9609" modified="2017-01-18" version="1">MPINLLHQIQQLLNWPQQLVAVEKRNVFVVYFLVLLATSLDNLNSTAALTMTANIEKEFHTDSSTASWVLSGYALTLGSFIMISGKIADIIGPHNLFLIGLTSVWICALICACIPHSSIITLIVFRAIQGIGASSLVPSTIALAANYFTGRYAKYLPAAIIGFIISLTGTLGFGIILGGAFSETLIGYRSFFFFVFAFGFFCDLVLLFLIIPIKKTTEHANLKMKNIDFIGSALVIVGILLIILGLTEGGENWRSPKAYMPLIVGFFTFVCALVFEILYIQRFKTKHRHLDKASDWRLQMELLFPPDIIRIPNFLPFLIVCGLYYATFTMMLAVGIEYYSFIEEASPIISAIKVFPLTVGLVFGAIIYRASYYEKVGLRNMFILSAAFTLGSCVWFSRTNYEVLNSYWKFGIGSLFLYGYGMNIFFNIYIGAVVANTPLHLQGVVNGIYQTCSQVLLSIGNALVPSILGNLQRATNERMKHELHAKFQSVFYVIMGFHATVFFIMVLFVRNLKKKEEPESEMIDDSVKNEDVCVSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDE6</accession>
    <name>A0A1E3NDE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18584</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015239.1">
        <property type="nucleotide sequence ID" value="XM_019162070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDE6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDE6"/>
    <dbReference type="GeneID" id="30178757"/>
    <dbReference type="OrthoDB" id="270624at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="310"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="601" mass="66909" checksum="53E1B842B45DE7BF" modified="2017-01-18" version="1">MISATAWVPRGFASEFPQQYELDDEEMKRIEAMSKLNLEEAQADLEYAEEEEAEENQIDSDNLKDQVDIDGDLKEYDFEHYDDDEIDETGEKVFMFPGLSNTRAAYVKTVKKNNSKGDDDDEMEGSEDGDVEEIEAEEEEHYLQLPTEEEKLAERAELQIYPTDNLVLATRTEDEVSYLDVYVYDDGAGALGPDDKEAEGEKKYDEDVKRGMVREGNLYIHHDLMLPSFPLCVEWLSFKPKGENDVQNVGNFAAIGTFEPTIELWNLDCVDKAFPDVILGEAEYAEWSSKQSGSKGKKSKKKKKVGKVYSKCHTDAILSLSHNRIFRNVLCSTSADSTVKLWDLTKCRVERGIDNGLHGGKAVSNSKWMYSNGDEEGGSILLTGGYDGYMRISDVRVEDVLKNNKQFSCGADYGEEVETVSKMGEHYILGGCDSGNVYCYDIRKEDEKPLWTLHAHDSGISTLSSNSEIPGLIVSGAMGEKVVKVWNVKDSKPRMILSKDFGCGNVLSTAFADELEVSGNLILGGSGNSGLKMWDCFSNPTINKSFHDELAGLKKRAVEEAAKYGRVSRIARKYQKGYGESVVEPEVGGVGGGDDDDDDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDF1</accession>
    <name>A0A1E3NDF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c oxidase assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_18603</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PET117 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015260.1">
        <property type="nucleotide sequence ID" value="XM_019162077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDF1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDF1"/>
    <dbReference type="GeneID" id="30178764"/>
    <dbReference type="OrthoDB" id="76305at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031568">
        <property type="entry name" value="Pet117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163:SF1">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15786">
        <property type="entry name" value="PET117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10691" checksum="BB064C7526632C09" modified="2017-01-18" version="1">MSRASKITFALSTLLSAATIGGVYFMAEYEKDQLQEGPIKDRARMERRALNTKQQANLEDYEKQKQIYAELEKTQPLTGEVVEGLDAKGLSPPKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDP3</accession>
    <name>A0A1E3NDP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="2 6">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_37592</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DTD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="2 6"/>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015353.1">
        <property type="nucleotide sequence ID" value="XM_019163092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDP3"/>
    <dbReference type="STRING" id="763406.A0A1E3NDP3"/>
    <dbReference type="GeneID" id="30179779"/>
    <dbReference type="OrthoDB" id="275783at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097358">
        <property type="term" value="F:D-leucyl-tRNA(Leu) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900832">
        <property type="term" value="P:D-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900829">
        <property type="term" value="P:D-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00256">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472:SF5">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="D-TYROSYL-TRNA TYR DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="162" mass="17743" checksum="397308EE66FA0082" modified="2017-01-18" version="1">MKVVIQRVKKASVTVDNAVVSEIKKGLCLLVGISTEDTVEDVTKLASKVLKLKLFEDAEQAAGTNTEWVGKPWMKSIVDIRGEILSVSQFTLYASVKKGAKPDFHKAQKGHAAVELYTEFLSQLKKGMHSVESVQDGQFGAMMDVALVNDGPVTIVYDTRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE11</accession>
    <name>A0A1E3NE11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate-semialdehyde dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="9">1.2.1.16</ecNumber>
            <ecNumber evidence="3">1.2.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NAD(+)-dependent succinic semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_37171</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="9"/>
    <dbReference type="EC" id="1.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015475.1">
        <property type="nucleotide sequence ID" value="XM_019163063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE11"/>
    <dbReference type="STRING" id="763406.A0A1E3NE11"/>
    <dbReference type="GeneID" id="30179750"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="44"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="507" mass="54343" checksum="9421FC2ADA0DE116" modified="2017-01-18" version="1">MLRRLYSTQSQNVVQTLAAVKKQELVQTIPFIGGEFSADNGPLFGVTNPYDGSELTKVMATTESQLSVAFENSQDAFDRFKKTTPRWRSTLLRKLNDAILANADDLARLISLENGKPLADSLGEVKYGASFMEWFAEEAPRINGLLIGSADPSKRISAYRLPVGPVGILTPWNFPLAMITRKLGAALAAGCTTVIKPASDTPLTALAFAKLCQDIDLPSGVVNIVPVEEARTPDFGRRFCEAAELKKISFTGSTRVGKLLLQQSADTVKKVSMELGGNSPFIVSAKTKDIEKAVDGMIAAKFRSSGQTCVCVNRVYVADAIYDDFVRLLLDKLSSTVQLGPGMDPTTTHGPLVHQRALDKVSGLVSDAVAKGATVLLGGNKRPDLGPTFYELTVLGDVTPEMEIYETEIFGPVASLIRYTGSMASVVAQANRNRDDVGLAGYVFSDDVTECRTASEELAVGMVGLNSGLISEAALPFGGVRCSGFGREGSVFGVEEYTVVKSVVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEJ2</accession>
    <name>A0A1E3NEJ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_36686</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015663.1">
        <property type="nucleotide sequence ID" value="XM_019163026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEJ2"/>
    <dbReference type="GeneID" id="30179713"/>
    <dbReference type="OrthoDB" id="4078936at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="218" mass="25432" checksum="F90DC8E15C7E9B18" modified="2017-01-18" version="1">MGEDQKQYFVPNEGEDYTIKYRTHGTSFYSLRLDKKYRLGNLPIYQNPLINFVIGSKRLSLAFGAIGCMFGYLMDRTGLVYTEISQLVAIFSLLPFPIVTYLFEPVVSRVWRVYDTTKPQIYENLIQDEQLIIEKLNWNGFNTYNELVRVDSLYVPKDKNDYRGRFGYANLLSVDKKLNSTKLYYINDGFTNEKMERILALAEKRSGVKDSGRSFYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEX9</accession>
    <name>A0A1E3NEX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_175240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015795.1">
        <property type="nucleotide sequence ID" value="XM_019161634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEX9"/>
    <dbReference type="STRING" id="763406.A0A1E3NEX9"/>
    <dbReference type="GeneID" id="30178321"/>
    <dbReference type="OrthoDB" id="422106at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019416">
        <property type="entry name" value="NCBP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10309">
        <property type="entry name" value="NCBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="449" mass="51524" checksum="EE27B96BC66A1733" modified="2017-01-18" version="1">MEIDQLVTSSQAPEEVEAADSKPPHEQYVAYESAVIEQEQQQEQQQKKENMEPILTSLHLTGVDDLSTQQIKKYLDYHIKPNYSFKTRKQFAYLEYRLDWINDSEINIVFDYNLSNGNGKRAKKNQPNDGVDLLDEIKGFAKKEVPQESVERDTDDEMKENLKEESVTETGSDTLQGLENESIRGAAEALLLLTDFEDIRKEHNEFAALSIEDQFKAVDQAPKLQDRKCWDLVLAKNGAVIKTTDAKRFYNLFEHVVQAKDSEDQLPNEEAIAGPTEVEEAEVFSEDTKIIKLEVRYSTELDKKVENARVFSRYYLLHGEPDKIERLPSARERLTGAYPKAAGYDRDLITAAEPETQGLFGYDGDREEREVIEPRYREREYRGKGINGRWSSDRFQAPGGHDSRHNGSRISKRGPRSDRRRAGRGNGNGHGRDQLEGDLFPDFAQRRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF10</accession>
    <name>A0A1E3NF10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_18078</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015789.1">
        <property type="nucleotide sequence ID" value="XM_019161870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF10"/>
    <dbReference type="STRING" id="763406.A0A1E3NF10"/>
    <dbReference type="GeneID" id="30178557"/>
    <dbReference type="OrthoDB" id="241990at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="766"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="1152"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="591"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="944"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="299"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="526"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="944"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="970"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1262" mass="139816" checksum="A8BDD0B4291D6E41" modified="2017-01-18" version="1">MTLPLDKDIIVNVLRETCLAATLQLANSLSTAQTSSDDDSGFANLQSPALFGRLYREGDISTKLDIQANFPLLAKLPNGSPPIRFDGLSENMSRRNKNESSTRGNILTEESNDDESDEEDEEEEEDEDQIDEDIEEEEEEGDDETDEEESSADDSNNANRKPFISTSPILNEVPSGLVDSRQRNTSSASTMSSIAPFRRRRRPLPSADHHKNPSSAISESTITETEDESSFSPPTQSEHQQIQKVSQEVTHLNSSLASPPSLQSKLTPIRRPDLGDRSDSVNTLKSKSELDPLINTPVSTKSSSKALISASTSKTEASSGSKKQTKGLRSLGRFIRPKQIASTFAHHSATNSNANNLVFGSDGSEEEPHKNSNSGLNDKTLNQAQQPESSMMEKNGSFDTKKLEGAELDSVPVLKVDSLSDTASAYAASSQLHSSKDYDEDDSKSNSERDTINSSDFEDNDSVVDSIESSELADSDYDYFNPPTTANTTRTEKRVSLAKGVSGDAGEVSDSALLGVGPNGDDISPGTSTGSSLTSGLRRSRGLYHPYEDPDVGDYIDDLDSEDYISDAEDYPIDDSVGYYDGDVLSANSAVGLGKDNRRRDSSSVNSTSSDMKNKLEVSPKMNSISKINFVRSRPGMRSTSGTGISRSLAPARRRQSIGSVIRPNTLLMSDTGSSLTTHMQSNLNTIDATSISSTSTANTKSLSKGVKSAKSYNNLLSMKYDSGSEEKEKQKEKNKEGNKLFFNKIDINKNTNNNLPSHSSKQKTSKLTELIHKKNISLDYYKYVMEKQSVSDTLLRLKICIPDLGIPYNQSLKLQVNSSVQVVELIGYVLYVLQQSKNESLDKHHLDPNAWKLYLADDDGEMEDDFGVLDRTRVVQSYGADEFVLVECTDVETKENEKVTPSPLENGSSQNLSDINPQMSANVSGIINYDDATFDAAQQTKSDSVATLPSELTPTSGLTFTNTLPSNNFQQQQQQKRQQVQQQRQLKQQQQFVLDDLDSDDDNLVFNEKNQKQVKKDIRKYAYLKSKLNTGSNKVGFNDKEREKESKLNKFKTRSKTIQSILHNTADHYQQTQQELFPSGNNTGNNMTFGSIMGNMNGVTVNNGVMMGATGVTSPNGVEIVNATNSTMAYSKQKNNNYLVEGEEGDNKALMYHRWTVWRRQQMSFKNKLPKTLTVDGYQIYLLPFNEFKGSWYESKTYNFDISQILKIKQNPKVPNYFKIIIKKNAEGIVKKYYLEARSSKECREIVDTIKALAKTYSEQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF91</accession>
    <name>A0A1E3NF91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_36600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015920.1">
        <property type="nucleotide sequence ID" value="XM_019163019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF91"/>
    <dbReference type="STRING" id="763406.A0A1E3NF91"/>
    <dbReference type="GeneID" id="30179706"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000306">
        <property type="entry name" value="Putative ubiquitin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="82" mass="9046" checksum="EEA570E96EFAC3C8" modified="2017-01-18" version="1">MVEIRIRSLTGRIIPVEIGLEQRVSDLKEVVEVKEGIPPQQQRFLFAGRQLDDDRTLADCGVAAGSDLHLVLSLRGGARHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFF6</accession>
    <name>A0A1E3NFF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PH-response regulator protein palI/RIM9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015973.1">
        <property type="nucleotide sequence ID" value="XM_019164548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFF6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFF6"/>
    <dbReference type="GeneID" id="30181235"/>
    <dbReference type="OrthoDB" id="3998164at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF8">
        <property type="entry name" value="AEL027WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="56195" checksum="54168DAFD3A93E32" modified="2017-01-18" version="1">MRPSVFTIIPQLVPLAFLILTSISSPTLTNIYLAKDTKNRTYGIFGYCSGDSHCTPVSLHTQLSKVLSSSVLPSSAIDKLDPYLIIAPIAAGLTFISVLFNVLALVFNGSAGFHSRVYWAFAMTVTILAFLTSSFVCIVTFLLFYPHVEWLAWCLIPSAAINLGAVVSLAFSFNSLCTPDKEMQTDDESEYNEKASVLNPPNIKSFNPYLNSDATLLNNVPPITKSNMNVMTRSLNSSASSLYTKEQDKNDSVVVTERINTKNSSSNMSLKVPQISNPYLNQGANDSVGNSNNSNLFPNESSNRNNDNSADDEPDQRLSDTENNSANSSSSNFTSISQRPINPKYYAGSPPNNRLPLSAANQYGSNGPQMKQNTMTPQQYHQQRQYNQYQNQMSSMPMQNKNAYMRKPKPYMVMGHRPPFPNQQPPAQRYHNGFNQMAQQAHNQPHQPYSNYNTQNQQFNQLNPYAPNSSKPRGNGFVPMKYRARNNMANLPASALGNDGPYSGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFN9</accession>
    <name>A0A1E3NFN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase Rpb4/RPC9 core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17457</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016064.1">
        <property type="nucleotide sequence ID" value="XM_019161611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NFN9"/>
    <dbReference type="GeneID" id="30178298"/>
    <dbReference type="OrthoDB" id="2186918at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990328">
        <property type="term" value="C:RPB4-RPB7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045222">
        <property type="entry name" value="Rpb4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21297">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="5">
        <location>
            <begin position="44"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="191" mass="21293" checksum="DA83A306ECA60F8F" modified="2017-01-18" version="1">MNISTSTVVTRRRGGKAHQPEDEENAAELKLGPEFQLEQVDHHGDVSKLITLNLSEARILIRAALKERMEMISKLNGVEIDTSDPSGEPDDDQLAKLSTAPGANEILRKTLDHLSMFSRFKDVETCTAVETLLKSEENSILHPFEIAQLGSLSCEDIDEAITLIPSLNDKKDQLDLQRNLDELNRLESNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFW7</accession>
    <name>A0A1E3NFW7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016143.1">
        <property type="nucleotide sequence ID" value="XM_019161636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFW7"/>
    <dbReference type="GeneID" id="30178323"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="60" mass="6879" checksum="2EAD8D097DE1C066" modified="2017-01-18" version="1">MTKSIIGSELVAFSHQRLFNGLAFLKLKSLIKVMTKFKKGFYNRIVHQRENVPIVSADDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGG1</accession>
    <name>A0A1E3NGG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome maturation factor UMP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74671</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the POMP/UMP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015546.1">
        <property type="nucleotide sequence ID" value="XM_019164839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGG1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGG1"/>
    <dbReference type="GeneID" id="30181526"/>
    <dbReference type="OrthoDB" id="15001at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008012">
        <property type="entry name" value="Ump1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828:SF3">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05348">
        <property type="entry name" value="UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16817" checksum="12C8881805536988" modified="2017-01-18" version="1">MPLSLIPESSQPSHVRNTQFAPAAPSAPGSIDTLRAQGPVSIASQINNRHPIESRILNWDATTTRLQMEIRRRLLGMADPIKREMELELVQQSEFRPQVLGGSARVHSDILRNKDWSVDWEDVYPASSDLLLHNTVSSALHTELETAFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGG6</accession>
    <name>A0A1E3NGG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74016</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016358.1">
        <property type="nucleotide sequence ID" value="XM_019164647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGG6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGG6"/>
    <dbReference type="GeneID" id="30181334"/>
    <dbReference type="OrthoDB" id="74910at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000003">
        <property type="entry name" value="Fumarylacetoacetate hydrolase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="12"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="234" mass="26165" checksum="0891401683D5634B" modified="2017-01-18" version="1">MSFNYIKNAGKFLCIGRNYMAHIKELNNQKPTEPFFFLKPRSSALIPGQGPIQIPENVIAHHEVELACIIGKKLQNLDPDKFGPDEALDAIDGYALAIDMTARNVQDAAKKKGLPWTIGKGFDTFMPLSPFITKAQIVDPYAVELNLKVNDEHRQSDLTDLMIFPIHKFISKMSSIMTLYPGDVILTGTPKGVGPVRPLDKVEAWCTHKGELVDGSHMKFDVIRKPGPYVFKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGI3</accession>
    <name>A0A1E3NGI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secretory component protein SHR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18354</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015566.1">
        <property type="nucleotide sequence ID" value="XM_019161964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGI3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGI3"/>
    <dbReference type="GeneID" id="30178651"/>
    <dbReference type="OrthoDB" id="5229808at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013248">
        <property type="entry name" value="Psh3/Shr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228:SF1">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08229">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00786">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24271" checksum="80081888EC3A58DA" modified="2017-01-18" version="1">MSTILDSLVTGGTLMAITSCSFFLGLLYSDWPYTHFLLWAAEPENADKVAKLALQHYVHWLDVPKFIPYTMYGVLLFGFLGFTMKVFKPAEDVKYFEYGSFAVYVLAVCCYASNVRFGVYSAQAGEWGDVDEFTGLSVIAASEVIIVFLLLGIVVIQSGIFFAKYEDDRVKAAFLMKELKVKLAQAEAQAKKGGVAAAASSGKASGRASGSAKGETAGSVNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGM6</accession>
    <name>A0A1E3NGM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17728</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3YL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016349.1">
        <property type="nucleotide sequence ID" value="XM_019161711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGM6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGM6"/>
    <dbReference type="GeneID" id="30178398"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11842">
        <property type="entry name" value="SH3_Ysc84p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11525">
        <property type="entry name" value="SYLF_SH3YL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000100">
        <property type="entry name" value="SH3 domain-containing YSC84-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033643">
        <property type="entry name" value="SYLF_SH3YL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF2">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING YSC84-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="362"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="296"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="421" mass="45535" checksum="4DC1D116F5F94C3A" modified="2017-01-18" version="1">MGINNPIPRSLKSESIKAAKVLASFIKPNQVLGADEIIPHEVLLNAKGLAVITVLKAGFLFSGRAGSGIVVARLPDGSWSPPSAIVTAGAGVGGQVGAELTDFVFILNTEAAVESFAQYGSVTLGGNISVAAGPLGRNAEVAGNASTRNVAAIFSYSKTKGLFAGVSLEGSAIVERREANRKFYGDMCKARYILSGRVAPPPACEPLMRILDSRAFSRLGPDDYDDEDYYDDIPSTFSSDDDSYMSSNQRRNMGRSGARRRNDDYDDDDYSDDSAGAYYKRDRARHTRGASWEDEVFDNDSGRASSSRRDDSGDRVYDRGSSRAVSGDRGYDRAERSYDRSDKGYRPSDDKPDFGRRAASSGSKNKAVALYSFAGQQDGDLPFKKGDVITIIQKSNSTDDWWTGRIGNVEGIFPANYVDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGN3</accession>
    <name>A0A1E3NGN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_37275</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015616.1">
        <property type="nucleotide sequence ID" value="XM_019163070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGN3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGN3"/>
    <dbReference type="GeneID" id="30179757"/>
    <dbReference type="OrthoDB" id="9996127at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S26" evidence="7">
        <location>
            <begin position="27"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="189" mass="21689" checksum="CF3B4FBE382B26C8" modified="2017-01-18" version="1">MNSLRSFFRTFRTDYPVLSYSLKRTVTFLSWVPVFYVISEHVSYIGVIDGISMKPTFNPDFSTSKDRVLLWKFNVKKLENLNVNDVVFLKSPINPNQIYTKRIKGLASDLIIPRYPDTRSKALIPLNHIWVEGDNIHSIDSNTYGPVSTGMVVAKAVWILWPLNRFGPVPSSGGRECRERFLRYPAKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH11</accession>
    <name>A0A1E3NH11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exoribonuclease phosphorolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74171</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016525.1">
        <property type="nucleotide sequence ID" value="XM_019164695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH11"/>
    <dbReference type="STRING" id="763406.A0A1E3NH11"/>
    <dbReference type="GeneID" id="30181382"/>
    <dbReference type="OrthoDB" id="27298at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="221" mass="24198" checksum="D55BDBAAF2C7F1B6" modified="2017-01-18" version="1">MSVVAKQNLLNQVDGSASFQFSSTKVISSVTGPIEVTKTRDEIPTRAHLSISVRPATGVPTTREAFLESKLKKIVSSMINMEQYPLSEIQIVVQVLETGEREQFTCKELSCAINSVYLALLNAGISLKSSFLSSFCICKGDNVIVRPTKDELDTSTSHHVGVYSIVDGKADELLYSDSLGYTSESELFTALTAISGDVNKINETIRDAVLNSVVEDYVWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH45</accession>
    <name>A0A1E3NH45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thiamine transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016583.1">
        <property type="nucleotide sequence ID" value="XM_019161815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH45"/>
    <dbReference type="GeneID" id="30178502"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF15">
        <property type="entry name" value="NICOTINAMIDE RIBOSIDE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="65839" checksum="B93D15F58BB2A039" modified="2017-01-18" version="1">MTLADKLRWLEVPHPEKKVLTVLKNPDLEPMPEDRRLWGFWAYFGYWSVPNVSIFTYSIGSSLLVLGLNIQQTMGAVTIGNILIITYTTLNSTPGSKFKIGFTMCQRMIFGIFGSYLGILIRILLSIVYYASMSWLGALGVVVMLSSWSKSYMNMENTFPEGLNMTKRDFTAFFCFNVIEFFFFFIKPEKMGPLVNGSCFITLVAMLGVFGYELHKCYVLTGGPGSMWYEPVSLPKSEIGWAWLEAVTVFYGAVSPNCTNMSDYSRFSKNSKQMYLGIILSIAMTGTLIPIMGMVTASNTLENYGTAMWLPTDVCLQWMADDYSAGTRAAAFFCGLAFASSQLTFNVLANGFAGGMDLSGIFPRYINIFRGAVITALISWACQPWNFYNTASVFNSVMASFGVVMSPIMGIIISDFHLVRKRRIRVSHLYSLDKSKDFWYSFGFNWRAYLCFFAAMVPGLPGLAAVVNPSISLPIGLIHYFDNSTVFSFFCPLILYYVICRFFPVQGAGETDEIDYFDVFTDKELQELSMRPYEEGCEFEGMLPLDRMHTGSDASDFIEGIDGTDAAGSENDKDCEKVSYIFQQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH54</accession>
    <name>A0A1E3NH54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA topoisomerase</fullName>
            <ecNumber evidence="6">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74243</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="6"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016598.1">
        <property type="nucleotide sequence ID" value="XM_019164718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH54"/>
    <dbReference type="STRING" id="763406.A0A1E3NH54"/>
    <dbReference type="GeneID" id="30181405"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140226">
        <property type="term" value="F:RNA topoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000018">
        <property type="term" value="P:regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="7">
        <location>
            <begin position="2"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="8">
        <location>
            <begin position="168"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="644" mass="73433" checksum="0C9A3A7D5E1CB8A8" modified="2017-01-18" version="1">MKVLCVAEKNKISKSVSASLSRNNFQSRNTKSKYVKNYSFRFNFPQWDDCDVVMTAVSGHIMQKEIPSDYGWGKVPNDSLFRCPIVTRYGDDNAKKIAENISDLAKNSDILMIWTDCDREGEYIGWEILKAAQEGNPSFTLDNTYRARFSHLEGKHIYSAACNPVRLDKLAIDAVDTRMEIDLRTGYCFTRLLTDSFRNVIKPNIANDDNSNGNSAKGKKKNDKMISYGNCQFPTLGFVVDRFRRIKYFKEEEFWGIDLNLKKDRKKFPFIWERNRLYDRLQIVCIYQNCINEQSDHVVVKHVSTNPTSRYAPLPLTTVELQKDCSRYFKFSAKDTLQVAENLYTKGLISYPRTETDSFPRSMDFKHFISMQTASSEWGEYSSSLLDESNHKFRVPRRGKHDDEAHPPIHPVGYADNLTGKEKTVYEYVVRRFLASCSLDATGSMTSIKVQWSTETFHSTGLVVLERNYLDVFKWDHWDSSKKGLPSVAVGEHIQISDATISSGKTAPPQPLTETELISLMDVNGIGTDATIADHIDKILTREYIIKQKLGSGKSSKEVFHPTILGYGLADGFGKLGFNNISLTKPFMRKDMENDLADICQGSKPKDRVLKETLEVYRDAYTITDEKVSTLVNAYKSALNHSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH55</accession>
    <name>A0A1E3NH55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome-releasing factor 2, mitochondrial</fullName>
            <shortName evidence="5">RRF2mt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor G 2, mitochondrial</fullName>
            <shortName evidence="5">EF-G2mt</shortName>
            <shortName evidence="5">mEF-G 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MEF2</name>
        <name evidence="8" type="ORF">PICMEDRAFT_17949</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016593.1">
        <property type="nucleotide sequence ID" value="XM_019161823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH55"/>
    <dbReference type="STRING" id="763406.A0A1E3NH55"/>
    <dbReference type="GeneID" id="30178510"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051881">
        <property type="term" value="P:regulation of mitochondrial membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000514">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03059">
        <property type="entry name" value="mEF_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030851">
        <property type="entry name" value="EFG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="54"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="63"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="127"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="886" mass="97779" checksum="D2F749246BABD06C" modified="2017-01-18" version="1">MPPILKCLRGNLAALKGIRFIHSSFIPKMAAPAASNSGSSSSYESDLILKTSISAIRNIGIIAHIDAGKTTTTERLLYYSGLTNRIGNVDEGDTITDYMTQEKDRGITIQSAAVTLPWKDHKINVIDTPGHADFTFEVTRSLRVLDGAVTILDAVAGVEAQTEKVWKQAKDLNIPIIAYINKMDRDGAGYGRTVKEIVGRLGTRAILLNVPYFVKDSSQNMIFKGIVDVINKKLIIWKDADLDGKEVDVFDISNSACEYPEVHEEMLKCREAVVETLGELDESIIDNFLETEDYTQVSIPLLKKSIRHLTLESKITPILCGSSFKNIGVQPLMDAIVDYLPSPVETNPPAIISSTLGSKGHSRKTKNKKIKENAVTQSSSNSATMSVIDPKLGCVINNNKNLTTALAFKVINHPNRGLMVFVRVYSGKLQPNSTIINSRTGEKIKIGKLLVMNGDSPLEVKYLSAGNIGVITGTEEISTGDTIIAHSLTRNVTQISKAETGLKLLPIDIPPPVFSVSIEPTTVADKRKLDTSLDILLKEDPSLKLSFDEESGQSILSGMGELHLEITKDRLVTDMKVKADIGDVCVTYKETITKPVGPVTIYENGAASLNEVSESQFGISLSVLPFDGEIDDMLVNMHEFMGKKFDEETDVHYLDMNTAVVFDKDSMPPVIRKELSLEKWTLNFSYSKVINSMLSGATGALQMGGRLARMPIQNIIVYVDKWYLPEVKDVSSVSPMIKVTREAIKSALSQLKDEDFTILEPIMNVRTYVLEADIGNVSQDLMSVRNAKIMGIENESEVGTNNDAQDLHWCKKQMTETYVPFDATMQYLQNNNGVSKMVISSEAPLREMIGYLSKLRSLTKGRGLYDMELKGMERASLDRVRQILDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHL2</accession>
    <name>A0A1E3NHL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_16930</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016748.1">
        <property type="nucleotide sequence ID" value="XM_019161398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHL2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHL2"/>
    <dbReference type="GeneID" id="30178085"/>
    <dbReference type="OrthoDB" id="438179at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="14">
        <location>
            <begin position="107"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="803"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="751"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="830"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="841" mass="95525" checksum="070CF61685240992" modified="2017-01-18" version="1">MFWVDSWMRKMMFFFSTGKSYCIFVPSILLFAKKRQFMLKSISLIKLRRPTYFSRKAVDNKKVLQRYMSESNTSYTATKWFKPETKVPVGYPVLKLYNTLTHNKVEFVPNEPGKIKWYSCGPTVYNSSHMGHARNYVSIDINRRVMQDYFNYDVTFIQNVTDIDDKIILKARQEYLFDEYVQKFEKKVSEDLITKSKSSLQAYVSKNLSEFTGKNVESEFKPWVETLDLKEIGLSKPKFPMHVKAVLAALEVIENPQTVQFDEFVSKVKDVIVPVLDAELGSTVTDPSIFRKCSSYWERQYDLDMRKLNVLEPTVVTRVSEYIPEIVSFVEEIVQRGYAYATSDGSVYFNTGKFDGSANHQYAKCQPWSKGDMALLEDGEGSLTNQASIDSKLNASDFALWKSSKAGEPFWDSPWGQGRPGWHIECSVMGSDFVGDKMDIHSGGIDLAFPHHDNEMAQSEAHFDCQQWVNYFLHTGHLHIEGQKMSKSLKNFITIDEALSKYTSRQLRLCFALVQWNNPLDFKETLLNESNSVESTLDKFFGKIRALKRDNEEKLDGGEIISKKFGPLEKKLFKDFGGSKADVHAALCDNLAVPQAIRAMMELVNSTNIYMNEVGGHELKIDLLVEIVKYMTRMLKVFGFDVREDGLGWKDVRGSGASAGGAGAAAGSVEEVGMPFVKVLSKFRDQVRKLSIEGGLDSRTLLDSCDRLRDEDLLELGVALDDRSDGQGALIKFLNEKEQAELLKQQRDKAAAHAARQLKKAEQARAAAAKERERLERAKVSPLEMFRGPGLEHVYSQWDADGVPTHLADGEEVSKSARKKLVKQQQQQKKLHDAYLKETAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHQ3</accession>
    <name>A0A1E3NHQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016739.1">
        <property type="nucleotide sequence ID" value="XM_019164393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHQ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHQ3"/>
    <dbReference type="GeneID" id="30181080"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="446" mass="50212" checksum="A55F9EFE8491663F" modified="2017-01-18" version="1">MSRNLSYTEPVPLEGGMKVGRRASGMQQQQQKAPKQTAYSVRVKGPSNFEVFNACVNDLTGKDKLAKVVLYTIRLLSTVHNVHGLGNPAVARSVRSALVMGKETLSADNSFVLNTSQTALCDENEGRGFSAFGSVLRLLAKVLLRPSNALSATKLERGLLVFLAFVVEKLSGLMAGLSVYRHLMRAGTIPFRVWKFSNHIRHSLRLLLDRSKLDGPATRVQRVLNYWTTKDMISQMANFWYAISDELMLVFRFNILLDGKHGGGAFTSALYSWAEDQELYSWMATILLGLNNDWNKWILLKDEQSRIILNQKVRARTRRIVGDLKRNNRMAQSRDLDPEEEDYDDEKDAGGDLTLSYVDQLDQNRKNITTVQINAIRLSCDLVFDAQYIFQWNMYKPFHVSLGLLSGALGLYNLWRDQKDRLTQKAVLAAIEAEAAIEAEAIVADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHS3</accession>
    <name>A0A1E3NHS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_43992</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016790.1">
        <property type="nucleotide sequence ID" value="XM_019163119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHS3"/>
    <dbReference type="GeneID" id="30179806"/>
    <dbReference type="OrthoDB" id="5571754at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3370:FF:000001">
        <property type="entry name" value="40S ribosomal protein S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_eS24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="135" mass="15308" checksum="8CBE61C39D97BBFB" modified="2017-01-18" version="1">MSDAITIRTRKVLSNALLSRRQFVIDVIHPNRANVSKDELREQLSALYKVEKDQVSVFGFRTQYGGGKSTGFGLVYNSVADAKKFEPAYRLVRYGLATKVEKAARQQRKQKKNRSKKIFGTDRKAAKKAAKRAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHT7</accession>
    <name>A0A1E3NHT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73198</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016797.1">
        <property type="nucleotide sequence ID" value="XM_019164412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHT7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHT7"/>
    <dbReference type="GeneID" id="30181099"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19120">
        <property type="entry name" value="AKR_AKR3C2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044494">
        <property type="entry name" value="AKR3C2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="7">
        <location>
            <begin position="48"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="6">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="334" mass="37209" checksum="CA69C4D19AE62D20" modified="2017-01-18" version="1">MTAATAHSVPIPHSDEQIPVIGFGSGTSWQWKKKASESTAVSADKPSNVDPDLVQSIVDALETGFSHVDTAEFYTTRPDVGQAVNDYLKKHPEKKRSDVFVTDKYNSHPPDTPNVLADGSIRGPYASLKQGLQLMKLDYIDLFLLHTADTPDGLSLEDAWREMIRLRDEGLVKNIGVSNFDVPHLQQLQKATSVLPQVLQLEFHPYLQNQSPGVHEFCRNNNILIEAYGPLVPITKAKEGPLIPLLAELTAKYGVSETQILLKWAHSFGVVTVTTSSKIERMKDILGVFDLELSAEDLKKITDVGNSWFFRAFAIPPLPTWDEQLKKERNVLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHX8</accession>
    <name>A0A1E3NHX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_73263</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016868.1">
        <property type="nucleotide sequence ID" value="XM_019164427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHX8"/>
    <dbReference type="GeneID" id="30181114"/>
    <dbReference type="OrthoDB" id="302966at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039739">
        <property type="entry name" value="MAG2/RNF10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983">
        <property type="entry name" value="RING FINGER 10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="204"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="715"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="566"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="745" mass="84183" checksum="83FB41CD3B7B8A4A" modified="2017-01-18" version="1">MSPYRKEVKGDFAAGAASTLPVASANEGSSSNRRFSSSGNEAGSSGMKKGGSRNRSSQNHSKRSAKAKNSSSSSGRLNGNDKQQLVDDSDDLLSMIKSSSKKGMDISHLLTYNYSKEPTPYDDESHSGAGNGYRNDRGRTSRQRKKKRSAGSDIHLSGLSYINANYKFIVRPGGDYKLQLLDPNLPLDINDVVRVAVKQNDYHCPICMGDDFVAPRMTRCGHIFCYPCLLSMFDNVKHDKTKTSIGNMHNPSIFCPLCSEVIKEKFVLLPVLIEPFDGSTPKVEPETDVNLSLMHRGSSKIYSQPVSNFYQFKGFKGSIPWISKSCTPLNYKQYSPYVSNTRLMMCDSEFIKACFQKEIDDLMTQKLLDHEMYGDPGIFYDIAASNIKENIKSLAVDSAVDGPQPICEKEVESHLSNISLSSSSEVNLPSFKDNYYYYECTTNSHIHYFLSPLDVQVINSLFSLPDIKKLGTEQLALNPAYNMPLNLKVYLENIDSEEGKVTPELVSKYPFLGNLPYGAELGFLEIDWSKFDASFIPKDYNEADEEPIEEQKRKLRYPSQIPQYIRKQLQNRTREINNKRLNEERARVRGELRREKETLEIFNESNTRNMKQDDDEDLIDTLISTRQKNKQFVHIDTINTMPFLKGTSNSFDLLATDSDENNEFNDMGTEGSSSNPAFTMTTKKSVWGTHVPIVIDPEEEALREEETRQFEEMLRRAKDQAVESNVSKGKKGKKGRRVKMVPLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI07</accession>
    <name>A0A1E3NI07_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016867.1">
        <property type="nucleotide sequence ID" value="XM_019161437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI07"/>
    <dbReference type="GeneID" id="30178124"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133386" evidence="1">
        <location>
            <begin position="24"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="67" mass="6747" checksum="E423C673481D7588" modified="2017-01-18" version="1">MQCSGLLNVLLLVSLSVTDTASASTTNSDSMPLAPSTYSAAAGFAREVSHDYFVAGAALIAAAVILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI45</accession>
    <name>A0A1E3NI45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_158249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016126.1">
        <property type="nucleotide sequence ID" value="XM_019160982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI45"/>
    <dbReference type="GeneID" id="30177669"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16931" checksum="57282B24FDA4E76D" modified="2017-01-18" version="1">MLDDISNPSSSSALTHDNFASLLFHAVNRYKALPSGVKRASRRKEEKFRLFFSSGFLAALPFLLLLLLLLLPHPSFHFLRLLFTFSDFFSLSQTSFFFLSDNNNHQKTLAFILCFHRIWVSFISERVKIRQEEPNFGCVNLTTMFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NID4</accession>
    <name>A0A1E3NID4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17163</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017018.1">
        <property type="nucleotide sequence ID" value="XM_019161492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NID4"/>
    <dbReference type="STRING" id="763406.A0A1E3NID4"/>
    <dbReference type="GeneID" id="30178179"/>
    <dbReference type="OrthoDB" id="444809at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029190">
        <property type="entry name" value="Rrp14/SURF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029188">
        <property type="entry name" value="Rrp14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007019">
        <property type="entry name" value="SURF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369:SF0">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15459">
        <property type="entry name" value="RRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04935">
        <property type="entry name" value="SURF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA-processing protein 14 N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal" evidence="5">
        <location>
            <begin position="242"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="369"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="319"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="53414" checksum="20AD3AA09F812ECD" modified="2017-01-18" version="1">MGNSLEERLQQHSNSFDGLMSLIPAKYYYDAETSNQWQQKRKSKQEHQALKRRKLDPESSKNLSAKDVLKEAEKTATPVILPGEKFKMHQQALAKKVQENGNVDEEEEDDEVDDDEDDDEDVDEEMESDEETKKQAHSQNNSPKQEEDKDEELLQENVTIVFDDNGEELVGSYKKEELQQQQKQQQQNVKAGKKTLSKEEQEQRERKHKELKAKLQAKIQSMREKRKAPGTKAPGAATSRQQILEERRKKAELKKEMKRKEAESSDDDDDDDDDAENVEGEETQDNSSVMFGNIEFLDGERVSSDLTSSRRVGKKKGPSNKDIKGHLKKIEREKSRLGELDSDAKKKLEDKNKWQKALASVQGLKVKDDEKLLKKALRRKESKKRKSEREWKDRIQTVTDEKKMKADRRDENLRIRKANKGKGRKDQVKQLPSYKRSRANKKMPGGAKRAGFEGGIRSHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIH1</accession>
    <name>A0A1E3NIH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RhoGAP-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_21905</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017043.1">
        <property type="nucleotide sequence ID" value="XM_019162230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NIH1"/>
    <dbReference type="GeneID" id="30178917"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000755">
        <property type="term" value="P:cytogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090334">
        <property type="term" value="P:regulation of cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09392">
        <property type="entry name" value="LIM2_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000009">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF7">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="77"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="386"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="8">
        <location>
            <begin position="792"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="502"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="553"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="596"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="690"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="509"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="610"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1039" mass="117854" checksum="7C6CA86F9452EDA4" modified="2017-01-18" version="1" fragment="single">KKTKVCKVCSKPIIGTLVRAMGNIYHVDCFTCFDCHKPCSDKFFAADIEVDQNGKSITNQTINVPLCEYDYFKRIDLICHTCNNAIRGAYITALRHKYHSEHFFCEVCHKVFESDNYYANEGKIYCHFHYSKLYAFHCESCKCAILKQYVEIFRGGKQQQWHPECFMVHKFWNVDITVNALGLNIHSIDDVSSNPEKLYKVETNLEKLTISIWLTLSEFEESCASLISEMLHTATTNNKSKGLLVTSKLVFKIRCLFKSIESLFNYSTSKHILLNYSLPKYQNFSQLKKEPRSLTSKIMSYLTFLRDIDSEKLTVNKYSHELLSLISTIAHFIKLISRNALMHALEFNRLSVSLKPTDILLSKISKHEDYPQELLSLNSISVKSSDSCTSCNKSIEDECIMYKIGDSIEKRWHTDCFRCSKCPNNQQIPVSELLESSFNGKTNEVLCPSCASGDVNAQVGFHLVSRYMQLAYLLEIAIVRSKIVIEKHFEETRVNLPLVDNKQSNSVHRPPDNDSYENKVTEITRRRSQREARKLNSANREIRKSVILEAPVASSASTEENSVHSKSSKDSTISPNSFRTGFKGSLKLRVQDLSADNKNARKGYSNHDSQAQKDDENEKSFDSVKSSNYKQRGPFNAALTSKLLQNESSLTLDDIPRIVSSEQAREHRPNAFRFQRRDYSSAISNLPKPKVVKTKDSNNDKIPLPTPSQPNVVTSYNAINGKRYSEFSNSAHEYIRHIAAFALYDMFSNKMTLEECTNLIDVKKSLSFWEKIFGNGHSNESESRVSASVFGVPLTTLVNKYGMDSDLGVGSHKVRIPLLIDELINVMRTQDVSIEGVFRLNGNIRRLRQLVEAIESRPEHVPKLENETPIQLAAVLKKFLRDLPVPLLTFKLYDLFILSQKLGSGPSRERVLKLAYAMLPKAHRDLAEVLFAFLSWVSTFANIDDNSDNMGGSKMDTHNLATVITPNILYTKIDTATHTKGKSSSDGEGIDPSKLINSAGGENQFLAIEVVNEMIEMNDELSVVPEDLLRLYKLAGFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIL1</accession>
    <name>A0A1E3NIL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_148306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017082.1">
        <property type="nucleotide sequence ID" value="XM_019160556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIL1"/>
    <dbReference type="GeneID" id="30177243"/>
    <dbReference type="OrthoDB" id="3995864at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025204">
        <property type="entry name" value="CENP-L"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13092">
        <property type="entry name" value="CENP-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="343" mass="37948" checksum="1118E60686706386" modified="2017-01-18" version="1">MSKIFINHGFKCLSITTLPSLEEPSGKASASRKDLPRMIIESMCADVESVSTARLLNQIASVVVSNGLFTSAEMSRSSSLGGLIPLNSKLYSHGSNTSGQPEIETVRKLLEVKLKIDDVEDMDEAGVEAETAEKLLAGHFRFHLPGIIPPQLVILVRSPSRRSADGGDRPGKMLLFFHGNIKFLPIFTSHIQEGFDCTITDLVPDNATLQSCLDWCVVHDTLSSIGNIELWFGRLQTKGKLGTIVVHIEEKDMLEFRDAFQMEDGTDGEELSAVLNEYLASKTSISFDKLTLVKLKCQLFTLSVDGKVKFSSSMSHLGQRAKKAKGDNRLSIWWIIRRLCFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIY1</accession>
    <name>A0A1E3NIY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative lipase ATG15</fullName>
            <ecNumber evidence="6">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Autophagy-related protein 15</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Putative lipase atg15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_22915</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
            <location evidence="3">Multivesicular body membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Prevacuolar compartment membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="6"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017184.1">
        <property type="nucleotide sequence ID" value="XM_019162285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIY1"/>
    <dbReference type="STRING" id="763406.A0A1E3NIY1"/>
    <dbReference type="GeneID" id="30178972"/>
    <dbReference type="OrthoDB" id="58570at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034496">
        <property type="term" value="P:multivesicular body membrane disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046461">
        <property type="term" value="P:neutral lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050805">
        <property type="entry name" value="ATG15_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175:SF2">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26363">
        <property type="entry name" value="Phospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0072">Autophagy</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ46071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="401" mass="44706" checksum="DE90E7524711DD62" modified="2017-01-18" version="1" fragment="single">QIQRIKDRSPDSMESYIFHSLQHPEINTDDSLEWSIDDIVIPNISDSETILQLAKLASNAYARLPADPSWRDVSDPDSNFGKAFNDSDGFGWTESGLRGHVFVESIDEGKFERSPLIIIAIKGTSAAGLGSTGDDGRTVEMDKFNDNLLFSCCCARVSSLWTTVCDCYEKAYTCNQNCLERSLRNPDRYYKAVLDIYRNVREQYPENEVWVTGHSLGGALSSLLGRTYGLPAVTFEAPGEMLATKRLHLPMPPGLPAEMEHIWHFGNNADPIFMGVCNGASSSCGIAGYAMESVCHSGLKCVYDTVTTLGWHVNILNHRLKTVIDKLLMMSNETAPCVKPPPCLDCYDWTFVDHSHNRKRVTKSISHPSPTSSTLSSTSGTKKRKCLKRTWYGSCYKWSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ19</accession>
    <name>A0A1E3NJ19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_153194</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017242.1">
        <property type="nucleotide sequence ID" value="XM_019160766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ19"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ19"/>
    <dbReference type="GeneID" id="30177453"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000907">
        <property type="term" value="F:sulfonate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044273">
        <property type="term" value="P:sulfur compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="84"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="402" mass="46070" checksum="C484930E190F94DD" modified="2017-01-18" version="1">MTTLGSITYDEAIHFHNDSDAQKDGILTVSADNLERLDHPEWAPTWDVKDDHAFENPKPFRHTDRALFADPTFQSLTGDKNVSVRKVTPKLGLEVDGIQLSKLSDKQKDDLALLVEQRGVIAFRNQDLKFESFDTLKKWGEYFGPLHVHPTSGAPLHQPEFHLVFRRGSAEEQAKVFANKLHNIAWHSDVTYENQPPGITLFAMLQSGNSGGDTQFLDMFEAYDRLSPFMKQKLEGLKVLHTSKDQAYYAKLTGGIERKDPVDSIHPLVRYHPVLKRKGLFINKSFSRRILGLKVEESDNLLLFLLNHIESCLDAHVRMNWDADTVVIWDNRRVLHTATNDWDTDEIRHAFRITSLAERPVESEQEYENWTPEAEEKNIALTDYFVGLTPAEYYDKVISAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ40</accession>
    <name>A0A1E3NJ40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_152287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017199.1">
        <property type="nucleotide sequence ID" value="XM_019160726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ40"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ40"/>
    <dbReference type="GeneID" id="30177413"/>
    <dbReference type="OrthoDB" id="2103031at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="265" mass="30032" checksum="5A852AD1FA59FE26" modified="2017-01-18" version="1">MGLFWGSSKGSSYSDQRQAPAASTTGGGDNSDNIISEDLAQYLETRESQLSNREFKALLRRQSANAEASKQAVESSNGVTGEENDFLSMIQAKGPQAGTELSAGETAAQAQVEIPKLPTVISTANSMKKPKEYQNFEFDKYRRENDEKEVVLTNCSEIQHAFYDCLGRQKIWERVTAVARLDSDECTKLADFFMACTEIQKKAFLTFDYATLETVDEMKVASRKVDKVFSNAFHSVDEIRDKEKYMNYTKELRREREDFYAKFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ61</accession>
    <name>A0A1E3NJ61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_24355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017282.1">
        <property type="nucleotide sequence ID" value="XM_019162347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ61"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ61"/>
    <dbReference type="GeneID" id="30179034"/>
    <dbReference type="OrthoDB" id="10267474at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990113">
        <property type="term" value="P:RNA polymerase I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990114">
        <property type="term" value="P:RNA polymerase II core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990115">
        <property type="term" value="P:RNA polymerase III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23157">
        <property type="entry name" value="Prefoldin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15177" checksum="965FE1CCFCCC302A" modified="2017-01-18" version="1" fragment="single">VNLASLEPQQLVAFKQSTQQELIHLQSSYDALVLAQNKYRDCINSVKLIEQNSRAEGASGNEMLIPLTSSLYVQGQNRVDKFKIDVGTGYFVEKDGKEAIAFFNKRIDKLSGDSLKLQQLLADKVGLMQSIDTVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ65</accession>
    <name>A0A1E3NJ65_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_142558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016731.1">
        <property type="nucleotide sequence ID" value="XM_019160295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ65"/>
    <dbReference type="GeneID" id="30176982"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="123" mass="13022" checksum="3CDA433CCDB7106D" modified="2017-01-18" version="1">MSLFLSNAADAGIPHRRHVDCWAHAAPRAWPTQSKPASFADGQSRAAACCVQHAARCTLHLAAMARNNLADLGRSPGFAPRQLCTKAYARSPLPASGGGAPVRRCSFLAIRFSVSGDGRAAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ79</accession>
    <name>A0A1E3NJ79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73599</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017239.1">
        <property type="nucleotide sequence ID" value="XM_019164531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ79"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ79"/>
    <dbReference type="GeneID" id="30181218"/>
    <dbReference type="OrthoDB" id="193856at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF57">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="9"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="105"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="200"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32338" checksum="F95968878D8C5C5A" modified="2017-01-18" version="1">MPESKTEPPPRYAGFIAGVFSGMMKNAVGHPFDSVKVRLQTSSGRFTGPLDCTIKTIRNEGVAGLYKGFTPPLVGWVLMDSVMLGSLHVYRRFVKENFFPDAKKMPLVGHCAAGLLSGWTVSLVAAPIEQLKARLQVQYDAKSRVYTGPIDCAFKLWKHDGLRTLYKGLVPTMIFRTNFIVWWGSYEIITNYFTENTNMSKAAVNFWAGGLSATCFWITAYPSDVVKNVIMIDNVDNPRFKKYTDAVKYVYTERGGLRGFTRGFVPSILRSFPANAAALAAFEFVMRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJB4</accession>
    <name>A0A1E3NJB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017348.1">
        <property type="nucleotide sequence ID" value="XM_019161106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJB4"/>
    <dbReference type="GeneID" id="30177793"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="53" mass="6222" checksum="EC835E73E8F1BD98" modified="2017-01-18" version="1">MDWLVGKDWLVLGITEGPTKPKATTRRRDDAERNRRREERETVKSQRTLGALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJH4</accession>
    <name>A0A1E3NJH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ornithine decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_20203</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-ornithine + H(+) = putrescine + CO2</text>
            <dbReference type="Rhea" id="RHEA:22964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="4.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.17" evidence="7"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017408.1">
        <property type="nucleotide sequence ID" value="XM_019162153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NJH4"/>
    <dbReference type="GeneID" id="30178840"/>
    <dbReference type="OrthoDB" id="5034579at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000005">
        <property type="entry name" value="Ornithine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 C-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="13">
        <location>
            <begin position="7"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="387" mass="42200" checksum="51DE9A63AAA2AA3C" modified="2017-01-18" version="1" fragment="single">FYVCDVGEVRRQLRLWRSLLPFVQPYYAVKCNPSRGFLGALAQLGVGFDCASQNEIRAVFEAHEALGASVDTKRLIYANPIKPISHLRFAHSHNVGLTTVDSLEEVEKIARYAAGMDVLVRITTDDSSAQCPLSVKFGADVPYSTKIVDRCVELGVHVRGVAFHAGSGFEDASTLSKAVQDARTVWDYANGRQFARCDMLDVGGGFSKDGFVEPARVLGREIGRLFSDELASGEMSVISELGRFLSASCFTLATNVIGTRREASADGASAAKDDKIRVYLNDGLYGNLNCILYDHQEVEPVVITSAGRFVYGEDTPVSTESGTRQYSIWGPTCDGLDCIKKRCLLSHDVHCGDWVGFRNAGAYTSAAATSFNGFANEFECVFIDTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK25</accession>
    <name>A0A1E3NK25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">37S ribosomal protein mrp10, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017549.1">
        <property type="nucleotide sequence ID" value="XM_019161167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK25"/>
    <dbReference type="STRING" id="763406.A0A1E3NK25"/>
    <dbReference type="GeneID" id="30177854"/>
    <dbReference type="OrthoDB" id="2210at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017264">
        <property type="entry name" value="Ribosomal_mS37_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10184" checksum="72628C1152EF0782" modified="2017-01-18" version="1">MPAKHRPAVPPLPRLRVKNQVAKQQANPCLVIMSQMLNCWASNGEGNVACKNLEQELKGCMAKGVKVPPPAKPTLNYHAARLLPKIHKQEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK64</accession>
    <name>A0A1E3NK64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72571</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017623.1">
        <property type="nucleotide sequence ID" value="XM_019164238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK64"/>
    <dbReference type="STRING" id="763406.A0A1E3NK64"/>
    <dbReference type="GeneID" id="30180925"/>
    <dbReference type="OrthoDB" id="2288928at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22652">
        <property type="entry name" value="NUCLEOPORIN NUP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22652:SF0">
        <property type="entry name" value="NUCLEOPORIN NUP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="370"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="516"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60777" checksum="CEC9143014F8070F" modified="2017-01-18" version="1">MAKPTSKSKKDTKQYTFTRRPIFNIHHSADPLFAAKCHPSEPVFVTGTATGHVQAYRYDIDKLEEIYEDQDKFPYDVSCVKYDDFDDDSIVVQWHTKRHKKSCRDLCFDLPSGGDKIYTLGSEGVIKSADLKTGQVVSKFSCDKDNSYDFQIGTFTKCVSPPGKNFLVVGDEEGNLMCHDTRGKHLQLAYKPFRKLHSGEGINSINYCWPKSEYKFITTGSTTVCEVDLRKPEEPLHTSEDQEDEVLCSTWLDQAKQETMICGMGDGIVTTWKPEMNRWEDQISRIRVAKNETVESLISAMDSDSRFMYGGCSNGHITKIDIKGGKVVERFLQNDPEDDDKVDEVLGLDLDCNYRLVSFGTDGFKIWEEESKNGGFGDEDEDEDEDEASGDSDSDGGFVSGASDSGEEEAENTVEMTLPVKLPAKRSLADSMKRRMEGTTSTSSKKSKTDPILEREEPEGQDESDIGVEVEDSDDDETEDSGRARQGKTVELHEIRDQILARISNPEIDGGDEAKRGKKAGKEKKKGKGAAPHEHGIRKFDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKJ9</accession>
    <name>A0A1E3NKJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_133414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017763.1">
        <property type="nucleotide sequence ID" value="XM_019160047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKJ9"/>
    <dbReference type="GeneID" id="30176734"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="136" mass="14559" checksum="9936C051908144D2" modified="2017-01-18" version="1">MQLRSSPVAIMCHRRRGRGGCAGKNLGKRPAQPTSGGRGHARGASAAAACVPLSCPFRSPCPTACIFRPPSSALRHAHSGTLHTARRTLQTICCTEARRRLRGHTAVCRGGGHVVPMAVCRCERGEREHGEEDRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKK5</accession>
    <name>A0A1E3NKK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_59649</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017201.1">
        <property type="nucleotide sequence ID" value="XM_019163379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKK5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKK5"/>
    <dbReference type="GeneID" id="30180066"/>
    <dbReference type="OrthoDB" id="3996497at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="44"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="190"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="351"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="450"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="462" mass="52240" checksum="1542ED7E56037B2B" modified="2017-01-18" version="1">MPNTFQGLLSFPEFSGNDLDSAGRSELVFQFHTILNNLSIQLCCPICQDVPTQPYLLPTCGHTFCYSCIKHWFQCNPSCPICRSIIGETKPILNHSVKNIIVALIDDLSQTAKELHLKDYKSYKKKLESWEKEKNEEFSVDKANDFPWLKKIASNWGRAVVDTEDGVPRCSACHWELVDGHCENCGRTMVGWQDRTDGDELDDDDDDEEDEDEFGRGPRRDRALHSDSDDWEDYDNGLNDMRGRIVDNEAEESDGGEEDGDGTEDNDAYRRRSHNERRDHHHGDETGYVDAYDSDDGFVVDDDEEKEEEDNDDDNESGDNEYVNTSDVEIVDEDKFASGDEDVILNANAAEPVYLSDDESDGGLINPRRSRALLPSDDDDVSEEEEDIEVHGGDSDDDDDVDDDLLKTVKSGRKKAILDDEDEDDDDDDNNDDAAGDDLSSDDLIPSRSHSSRHKKKKRRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL24</accession>
    <name>A0A1E3NL24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_51735</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017381.1">
        <property type="nucleotide sequence ID" value="XM_019163157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL24"/>
    <dbReference type="STRING" id="763406.A0A1E3NL24"/>
    <dbReference type="GeneID" id="30179844"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF10">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="733"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="959" mass="107237" checksum="80E5935DCEF22F8E" modified="2017-01-18" version="1">MSSETDKGGRSVSSVSALLETLGSRYTERGQAVTDSEFVNPLSKGQAAGFETKPIQSHTKDNEGTEGSVSSRDGLRAGEDTNTFSNYASIKNNAEDGDGSENEYGDESIISDKHSVSSSESRSLQNSNVRSLSPNSEGSRRRMLACKRCHSLKVKCVPLDTSVEFSTCKRCFKRNISCEYNTLHKRKKPAPLTNSMKFKIKDDEIGKLREELKRKDRLIKLLRGFTDEEEEKMVSEISMHDRLKLYGKEINELEAMLPSSSSSSQFISNSERRVQLGESQMSSLDIVGNNILTFQQCEKLLHLFLNKVYSKFPFIDLPSTLTVEFLREHEPFMFIIMVYIGTIADTEPSDISVESQLQLECLISKTIAIHALVIGNKSINLLKSTMLYSLWYTAPELFHQRRYHLFSSFCASMAHDLGVSGRPFFFYNKDDGLVKKTSVLGDLHTIKLKALILVVYISYMSTSLFLKRVIFFQWTEYLDNSCVLLEGSDLRSYRMIALYARMNHLMETIYCEVHKQSEQITVLELSSTRNKLQLVEYLNQLNGLKQKISDNFRQNSPDYSSLMAYMYSVQAYLYEPALQTLINSKEYVTPEYKNVFFSTLSQICESCLLSLKHFNQLSIEDMTSNPLFHVSRILYTSGMLLRIRYLSLTIPKSGKASLFTEECISTIRTLTAKVEKTCKMFPKNHALKKVANVLGLFVHTCLSQWYISYKSLSKEIKKHTPLFVDPLFDSDANPQGKRRATNAIDKPGSAASGDVSHNPAMSPDNSVINGTKHSAAFKPLISSLLQPQTDETTGPIASYKYGLNSQQHRGSAVYSVAPSPPTPVLHIASNSTGIPDGSRPDNSIMKFQSTPAYNNMNFNSSMNVGNLGSMPMEQQPTGLDQLAGIAVGDLGNGAATNLPGRVGSIEGIENNWEFQYMAFNDEFWSDLFFGNGESFTGPVGSNNGIIGMDPTPLNLQTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL93</accession>
    <name>A0A1E3NL93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_72929</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018012.1">
        <property type="nucleotide sequence ID" value="XM_019164335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL93"/>
    <dbReference type="STRING" id="763406.A0A1E3NL93"/>
    <dbReference type="GeneID" id="30181022"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="109" mass="12253" checksum="07D266B4B27CFEA4" modified="2017-01-18" version="1">MEEIATGLKDTNTLELPDTLSIQITVELKNDVKITGTLKSVDQFLNLKLDNIHVDTEKYPHFIAIRNLFFRGTNIRYIHLNPASVDTNLLQDASRREAMASAGEKIAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLB7</accession>
    <name>A0A1E3NLB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Golgi apyrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72965</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018055.1">
        <property type="nucleotide sequence ID" value="XM_019164345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLB7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLB7"/>
    <dbReference type="GeneID" id="30181032"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046036">
        <property type="term" value="P:CTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006256">
        <property type="term" value="P:UDP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24003">
        <property type="entry name" value="ASKHA_NBD_GDA1_CD39_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF121">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATASE MIG-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="634"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="752"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="210"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="808" mass="91006" checksum="AC53250B7F5C34A7" modified="2017-01-18" version="1">MPLLQPRESEQAGSARRTAAPVYDNDELPYNYIVVIDAGSKGSRAYVYAWESAKHHLGKERKAGSEAGAWGLPRLWYEKHWHKRIKPSIESSIIDFGLHNSYLYNYLDHLVAEVYALVPVEQHYRTPVLFHATAGLRALDPDLQSKILDKICGYLADHTDFFFPDCASHANILDGDVEGLYSWITVNYLLNGNRTAQNPASTYGVLELGGGSAQICFQPSAAETEKRSVQLVNLQLDQNYQLFSTSFLGYGLNQFHHDYLLFLIEKSSLAAAAADAAGAAEDGAYKNSGILDPCLPKEYSDTFTFNSHQYILHGSSDFQQCMDNIYSIIGDNDEQCKSLLVPDEDSTTVKVSKCMLNPIMPKFSLDKDNFIAISGYWDAVIQLLDFTNQNKDTVVSNSVVYHPEIFTETTEKICNMDFTSLQHYGGSDNHKRFSTDEITNMCFKSSYITALLHSGYGLPLNSDLDQDPDANKMANHLLINDKINGFKFSWTLGRALLYAADESAREYAKFVNGTDASLPPELRVGYYRNSAPTFFHHGSEQQGVPTRPDFELKDEYPTYTFDLKNQATKAGHAKPPATTTEQAMDYYYDFDNGYDEDEDSNFDNNGGFGDESCDDDDEAEFVYQSSNHSTAMKVFPILTLIMILLYFIPFTRNYMIRLYIKVRRSFSLYSSLGQSPDSFTFNSDVENTIGADGNLRKFHIRGGNKKNTDVQYDNNKSPTNGEDFHLEIPSAKDMRFSSSNDLELQDFKVGDFSLNPEDGTTNEPSTQDDDDRLWDVDNNLRNDVVSDFDFGVSDDEWENDSDGIERAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLG8</accession>
    <name>A0A1E3NLG8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_16788</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018105.1">
        <property type="nucleotide sequence ID" value="XM_019161345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLG8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLG8"/>
    <dbReference type="GeneID" id="30178032"/>
    <dbReference type="OrthoDB" id="10257471at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="128"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="3">
        <location>
            <begin position="239"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="3">
        <location>
            <begin position="384"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="860" mass="97446" checksum="A56A1EFB9782CF8F" modified="2017-01-18" version="1">MNQEGTGVGLFNDMNGQGGELAEAVSDGESVSISTNNLVVINNNKASSLSSSSSNNNNENPGSNGIGNNLGNGNAEDEMDNDPDTDNEQHARSQILRTRARANSHSNSQKTNPGRNPSLVQQNYGGPISSLPPEMLCLIFSYIHSKPDLISIALSCKYWADLIISIIWFRPGINNKHIFAKLEKTMKSSAIETMWNYRKFIKRLNLSLIPNLVNDDFLKLFIGCTNLERITLVNCSKITHESIAGLLEGCSRLQSIDLTGVTDIQDDIYMQLSMHCKRLQGLYAPGSASISQEAVLNLIYNCPLLKRVKLSDCNSITDDVVYALVKNCPSLVELDLHGCEQVTNKSLHMLFLQLESLKEFKISKNQNITYECFESPNGSILCLDKLRILDFTQCSNITDKAVIKFIQLAPKLRNVVLSKCSSITDASLRAIATLGKNLHYVHLGHCSNITDAGAKELIKNCYRLQYIDLACCNQLTNATVVELSNLPKLRRIGLVKCSQITDEGILALAENSRNVDDTLERVHLSYCVNLTIYPIYRLLRACPKLTHISLTGISQFLRPDITRFCREPPSEFNPHQKSIFCVFSGEGVNLLRNHLNHLIQTTEVEDREATEILQIINGIIGTTTPANMDQFSSDQARERFEHFVSNAMDFLHDYDGIAISVETMKLFARCIFGGIQQTQATRVQRFFQLMHDRPARTRMRGRYRQIQLQQQQQQQQQERDRLRELELERLRARPRILATPEDSVRFMLELQARNPTPEFHGSALENEIINRRYRTTLTPAEIRGREEMGERERRFLEEEQQRNIEQTQRLAIARDAGVLQMFGMNSEEEAEGRQYANIPPEAVELFREDSQDIDEEFMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLK9</accession>
    <name>A0A1E3NLK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]</fullName>
            <ecNumber evidence="8">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_72975</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="8"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018069.1">
        <property type="nucleotide sequence ID" value="XM_019164349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLK9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLK9"/>
    <dbReference type="GeneID" id="30181036"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="11">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="124"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="448" mass="49042" checksum="149BAE7376A35195" modified="2017-01-18" version="1">MSAKQILLLGSGFVAQPTVDILAKQPGVEVTIGCRTIESAKALAGDKAKAISVDATNQQELDEVVKNYDLVISLIPYIYHANVVRAAIASGHTDVVTTSYINPTLRSLEPEIKKAGIVVMNEIGLDPGIDHLYAVKTIDEVHREGGKITSFLSYCGGLPAPEDSDNPLGYKFSWSSRGVLLALRNSAKYFKDGKEVSIAAEDLMSSAKPYFIYPGYALVCYPNRDSTTYKELYGIPEAETVIRGTLRFQGFPEFVKVLVDMGFLKDEPSEIFAKPVAWNKAVAEYIGAKSASEDDIVAKIDELTKFKDDADRERILAGLKWLGMFSDTAITPRGNPLDTLCATLEELMQYSDNERDLVVLQHKFGIEWADGKKETRTSTLVDYGVPGGYSSMAKLVGVPCAVAVLEVLKHQGAFAKPGLYAPMTPEINNPIMKTLKDDYGIFLTEKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLP4</accession>
    <name>A0A1E3NLP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71188</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018178.1">
        <property type="nucleotide sequence ID" value="XM_019163855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLP4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLP4"/>
    <dbReference type="GeneID" id="30180542"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032984">
        <property type="term" value="P:protein-containing complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="70"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="279" mass="31831" checksum="63B5A2A5180A112B" modified="2017-01-18" version="1">MSGSPLEKIKSVFQKSSHVGKYSDSEKSYSNRSNSNGNSSVDSNSQLHEFEEPPLTPLSLSGYKSTTKHRLLNPELAEEIRQHIPSLLQINHKWQLLYSVEQSGTSLHTLYSNCHPLFGENMNRRRGYILVVQDTRKNIFGAYLNDYLRPLEGKYYYGNCDCFLWKLQKGKVMHLKSNNSTIRSHEGETARKEAHHGNDGREEEEVMNLKVFPYTSLNDFIIYSNNEFISVGSGDGKFGLWIDSNFEVGASDTVETFGNEPLSDHKKFHILGLEVWKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLP9</accession>
    <name>A0A1E3NLP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018170.1">
        <property type="nucleotide sequence ID" value="XM_019163853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLP9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLP9"/>
    <dbReference type="GeneID" id="30180540"/>
    <dbReference type="OrthoDB" id="102559at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF38">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="159" mass="18258" checksum="9ED6C43A2EFEFE09" modified="2017-01-18" version="1">MPSVFSVADIKYILDPTLKSWILQGHTNLGTPFQVIDVRDSDYVGGHIINSLHIESNKFKNEESAETLSNLLKKLRENGQNTVVFHCMLSQQRGPSSAMRFLRYLNERTSASNDQDEISFIENMKVCVLRGGFGKWQQEYGEDPQLTENYAKDVWKYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLQ5</accession>
    <name>A0A1E3NLQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15068</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018182.1">
        <property type="nucleotide sequence ID" value="XM_019160648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLQ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NLQ5"/>
    <dbReference type="GeneID" id="30177335"/>
    <dbReference type="OrthoDB" id="957735at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16979">
        <property type="entry name" value="VHS_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="13">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="170"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="272"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="657"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="674"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="699" mass="79173" checksum="1AD91AE3A13DCB05" modified="2017-01-18" version="1">MSWFGTAKYPQFEEKIKDATSESIPNGEIDFATALEVSDMIRSKQVPPKESMRSLKRRFLEAENVNTQKSAFKLIDFCIKNGGEHFINEIASKEFMDPLVAQLKEKTINEGLKSYLLEHIQTWSIMVSKDPKFDYINKTYRKLQDDGFEFPMISDVIDSTMIDSKVAPEWQDSDACMMCSKMFTFINRKHHCRSCGGVFCAQHSSKSVELPELGINIPVRVCDNCYTDQKAKRKKNKRNKRQSDSKSLDSRNVVEDTDDEIKRAIELSLKETAAKNSHDTTSKVNATVTQDEDEDEDEAMKAAIAASLQDLNNKNSNGSDKLEAIKEPEVQKDSSTGLYSNLMSDRPYVPTSSSLSSQHTVQERPPISYSQQQQYFQAPQPQAQLQPHSLSQPQLQLQHQKPYEQRRDDMYVASNSNTIRGIDETKVVDFIQLLDNLKGDQYRINTTDPRIIQLHSDMVLLHPKIGAAIANEHSEIEKFQSLYSKLFAISRLYDDILQSRLKQEQDMLKSQHNSLGPPQSYGRSPYQQHIPPYLNQPVQYLSPQSTFNQHPQVHQLQAYQSQSGTFQIDELPTHNIVNTSQSISPQATCISPSSQFTIPSTYPIPAPSGTNLEAQQLLQMVPSSPYSHKVSAEEIPRVKDFSDGKLPISEHLRYASVPETVSEGPPSPNQAEPKVSETEMHHVTAQLKKEPEIVNLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLR0</accession>
    <name>A0A1E3NLR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71206</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the profilin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018198.1">
        <property type="nucleotide sequence ID" value="XM_019163861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLR0"/>
    <dbReference type="STRING" id="763406.A0A1E3NLR0"/>
    <dbReference type="GeneID" id="30180548"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070064">
        <property type="term" value="F:proline-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140311">
        <property type="term" value="F:protein sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000001">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027310">
        <property type="entry name" value="Profilin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF0">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00392">
        <property type="entry name" value="PROFILIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01640">
        <property type="entry name" value="PROFILINPLNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00414">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="126" mass="13357" checksum="ECEC920B9EADE8F8" modified="2017-01-18" version="1">MSWNAYTDNLLKSGYFDKAAIFSADGAGVWANSGNLTLQPGEINAIVAGFNDASQLHMSGFHVEGLKQFCIKADARSIYGKHEQQGVMCVKTKQAIIIAHSPEGVQAGGAATVVEKMGDYLIGSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLX3</accession>
    <name>A0A1E3NLX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71256</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018258.1">
        <property type="nucleotide sequence ID" value="XM_019163874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLX3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLX3"/>
    <dbReference type="GeneID" id="30180561"/>
    <dbReference type="OrthoDB" id="2382881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF9">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G06390)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="38"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="143"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="259"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39107" checksum="E0B6E4F413214A3B" modified="2017-01-18" version="1">MSEEERRNMGSEESSRPIPGITGDIVYKNPLFTMPAILEPYKVGILSYASSMLSTTAGFPLDSIKTRMQTHSYTSALDCFSTTIKTEGVRGLFRGIVAPLISTSMSRSMTVSIYTDAKPYVASVMPEMQLDYISDPETQRFVRNFPVSFISGIISGSIISLFACPFELTKIFQQIVVVVNRDTKVNLSSETLPTKVFDVAGNIVKYEGWSGLYSGYRYHIIRDAFSAGLFYSVYETVKLQLQLKNKESDYFSEEVKARVDALCVPVSGAAAGCLAWATIYPLDTMKSRYQRDVLGNIIRVKMGLPKLDIAPGKLKIPTREMYKGFGPSVTRSICVTMIFFSAFEYLMKNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM13</accession>
    <name>A0A1E3NM13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_178191</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018219.1">
        <property type="nucleotide sequence ID" value="XM_019161759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM13"/>
    <dbReference type="STRING" id="763406.A0A1E3NM13"/>
    <dbReference type="GeneID" id="30178446"/>
    <dbReference type="OrthoDB" id="10260596at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_eS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_eS6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="57038" checksum="81FED1EA50B6E79A" modified="2017-01-18" version="1">MAALFYEPPPPRVSEILKLPDNLDFLFFYHVLLHRACSNPFEISTRTPCPFNLPRAPHTVRASCASFRRSSAAGLLAAALCGELWRRWRCERSRPCQDSDRSEQAEDCYRQKKVAFESSLGPLDIVQARLTHNNHEGMLDTAGEGQKANGRRRRLRKTEVVARGQHKRAEHEHEHEHEHLSVNMNTRSRTDKHTAKQSVENQQAQRRDIGRARVQSQKHTRKTQADGKKQAVGTVWRPLSRHALYTPQQAHNTNFHFPLSFLQLNIAYPPNGTQKCIDIDDEQKLRVFFEKRMGQEVDGDSVGDEFKGYVFKITGGNDKQGFPMKQGVMIPNRVKLLLSKDHSCYRQRRNGERKRKSVRGCIVANDLSVLALTVTKLGEQEIEGLTDAQVPKRLGPKRASNIRKFFGLTKEDDVTQFVIRREIVKGDKTYTKAPKIQRLVTPQRLQRKRAVKAQKIKNAQAQREAAAEYAQLLAQRLHERKAERAEIRKRRASSLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM29</accession>
    <name>A0A1E3NM29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear protein localization protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_19092</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018239.1">
        <property type="nucleotide sequence ID" value="XM_019162105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM29"/>
    <dbReference type="STRING" id="763406.A0A1E3NM29"/>
    <dbReference type="GeneID" id="30178792"/>
    <dbReference type="OrthoDB" id="10251089at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036266">
        <property type="term" value="C:Cdc48p-Npl4p-Vms1p AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000837">
        <property type="term" value="C:Doa10p ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000839">
        <property type="term" value="C:Hrd1p ubiquitin ligase ERAD-L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030894">
        <property type="term" value="C:replisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900182">
        <property type="term" value="P:positive regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072665">
        <property type="term" value="P:protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007716">
        <property type="entry name" value="NPL4_Zn-bd_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05020">
        <property type="entry name" value="zf-NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010052">
        <property type="entry name" value="Polyub_prc_Npl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="245"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="586" mass="64642" checksum="30EF93F75AE4BB63" modified="2017-01-18" version="1" fragment="single">MIIRFRSKDGQYRLSCEGDTPFQDVLSELVLTKLPPMDPQTLSVTMPGPDGGERSAGEVGTQTVDGLGLKNGDMLMLKYTASSGNEGGGQSISGKGSVPESVGISKVIDGSAASTSARAAAKQSALDDELDRDPGLIPRKRGPLCKHTDKGMCEYCSPLPPWDREYQADHNIKHLSFHAYVDELNAKVNKKEGGSSYIPPLKPSDFAINRRCPAGHEPWPKGICSKCQPSAITLQRQEFRMVDHVEFENSGMVNNFINAWRLSGVQRVGLLLGRYARYDKIPLGIKVQVEAIYEFPQVDREDGLVLQQWDDEHQVEALADELGLQPVGVIFSDLSDAGRGDGSVICKRHLRSFFLSSLEILFAVHWQLKYRNRCKWSETGAFSSKFVTCVVSGNSSGKIDINAYQAAESAEALVTADLVCASTHPDQMFIKPATPDRYVPDIFYQKINEYGLQVKHNAKPSFPVEFLLVSLTHGFPEKDTGLFRTTDATALAPSAFPVENREYLGDHADLRTLQTYFSCFHTPSNTDPDTFLAELSSFHVLFFLFCANSVLSDADRRVAVRAVRTQDPAVAVQLLDSDGFKTLQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMH4</accession>
    <name>A0A1E3NMH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71412</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018443.1">
        <property type="nucleotide sequence ID" value="XM_019163913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMH4"/>
    <dbReference type="GeneID" id="30180600"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="7">
        <location>
            <begin position="4"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="141" mass="16434" checksum="D7A1C0906A359241" modified="2017-01-18" version="1">MAARYIVCLNRQGKVRMSKWYERTGVAEQRATIGRIHRLIQRRLQFRSCNSVDFGADTQLVFKKYNGLYFVVCVGREENGLLYLQAIPLFVQLLDRYFDTVSELDLVFNFYKMHRVLDLVFVDGELVATKTEAVLAQLGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMT9</accession>
    <name>A0A1E3NMT9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Altered inheritance of mitochondria protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AIM11</name>
        <name evidence="8" type="ORF">PICMEDRAFT_10428</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the AIM11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018544.1">
        <property type="nucleotide sequence ID" value="XM_019159668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMT9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMT9"/>
    <dbReference type="GeneID" id="30176355"/>
    <dbReference type="OrthoDB" id="4088121at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038814">
        <property type="entry name" value="AIM11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="172" mass="19586" checksum="0B33BD03F6C95BC1" modified="2017-01-18" version="1">MSSSFDFGKLFGRGDPSKKTDAVLKYNERRFYQMATFYGFTLATYIASKIAYRGVIKRRYNPTFYQHNHVPPKFSFYKDAMVAVTHASLLATTSLGMLASGAFWYFDISSLKEFTYRMKIFLGGYDAEKALKAMPEDEETKQITDSLDSILNGNLLDAFDDRETKEDSASSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN48</accession>
    <name>A0A1E3NN48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16668</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017966.1">
        <property type="nucleotide sequence ID" value="XM_019161300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN48"/>
    <dbReference type="STRING" id="763406.A0A1E3NN48"/>
    <dbReference type="GeneID" id="30177987"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000020">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="227"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="450" mass="50327" checksum="D1EE06D7330278E1" modified="2017-01-18" version="1">MGWEKRGKAGKETDRNRRARNTQPDTMEELGIVPQSEHPHNKIDKLTAKAANGSLNKNANSNGNNNNDVYLRLKKLEKNLELILLQEEYIKDEQRYLKRELVRAQEEVKKIQSVPLVIGQFLEPIDQTTGIVSSTTGSNYVVRILSTLDRELLKPAASVALHRHSNALVDILPPEADSSISIVGEKEKPDVTYNDVGGLDMQKQEIREAVELPLVQADLYKQIGIDPPRGVLLYGPPGTGKTMLVKAVANGSTASFIRINGSEFVQKYLGEGPRMVRDVFRLARENAPSIIFIDEIDAIATKRFDAQTGADREVQRILIELLTQMDGFDQATNVKVIMATNRADTLDPALLRPGRLDRKIEFPSLKDRRERRMIFSTVASKMSLAPEADLDSLIMRNDPLSGAVIAAIMQDAGLRAVRKNRYMIIQSDLEEAYTAQVKSGSEVDKFDFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNK3</accession>
    <name>A0A1E3NNK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphotransferase</fullName>
            <ecNumber evidence="13">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_71350</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="13"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018371.1">
        <property type="nucleotide sequence ID" value="XM_019163899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNK3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNK3"/>
    <dbReference type="GeneID" id="30180586"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000805">
        <property type="entry name" value="Hexokinase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000004">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="14">
        <location>
            <begin position="12"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="15">
        <location>
            <begin position="216"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="480" mass="53474" checksum="0360E858B3F2D0F5" modified="2017-01-18" version="1">MTVDKTELDKSIKKVQAEFWLPTETLDKIVEYFIEELERGLVDGTDPLGIPMNLAWVMEYPTGDETGDYLALDFGGTNLRVVMVHLLGGKKLETEQSFYRLPHGMRTTKDRNELFEFMAECLEKFLIEKHPSGIPENKTFPLGFTFSYPATQSRIDAGVLQRWTKGFDIPGVEGEDVVPLLMEKVNNRNLPIKVVALINDTCGALVASRYVDPLTEMGCIFGTGVNGSYYETVNAIKKVKGQLPADIPEDSPMLINCEYGSFDNAHKVIPRTKYDLKIDADSPRPGQQTFEKMTAGFYLGDLMRLVLLDQYKNGLLFQEADSKLIKLLETENCIDTSFLSLIESDESEDLSLVKKTFADALHYEDATSDECYFAKEVAEIIGTRAARLSICGISAACKKRGYKNCHVAADGAVYLKYPNFPERAAEGLADVFRWGRDLPYDQHPIKIVKAQDGSGVGAAVIAALSHKRQLQGLSLGLKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNM8</accession>
    <name>A0A1E3NNM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_32455</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018807.1">
        <property type="nucleotide sequence ID" value="XM_019162767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNM8"/>
    <dbReference type="GeneID" id="30179454"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF54">
        <property type="entry name" value="PROTEIN ESCARGOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="222" mass="25368" checksum="63E2A8D48B899AD2" modified="2017-01-18" version="1">MKIEEKEREVKREQLRAQSQLNNPRSKAKRAKAAGKGEAKVVKKRKVFRCSFCNKVFTRKSNLDSHLITHSTERPHVCQECGKSFARLSDRTRHESTTHKATKTFQCRGVKKDGVREWGCGHFYSRADGLRKHFKSFAGKQCLYDFLRDLHGDTDELFIAFRGKPSQHQAAVTAEDGPQTKDTPATPTANTDIMDNSTTFDSPEMLQNVEKAIRNVRQHCGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNN0</accession>
    <name>A0A1E3NNN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15616</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018809.1">
        <property type="nucleotide sequence ID" value="XM_019160892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNN0"/>
    <dbReference type="STRING" id="763406.A0A1E3NNN0"/>
    <dbReference type="GeneID" id="30177579"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05384">
        <property type="entry name" value="CAP_PRY1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000012">
        <property type="entry name" value="Secreted protein PRY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133459" description="SCP domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="7">
        <location>
            <begin position="127"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="262" mass="26917" checksum="41BEEBCE80696916" modified="2017-01-18" version="1">MQLSKISVLSLFAAVATTAPVVETVTNYYVTTVIVEGQTSAVNDAPVFSTSATATAAAAATSAEATPSPTTFETSAASTEAAAEASSSSTVADYQQKQADQPASSSTTSSSAAAPSSTGDSGSGGSDFSSQILAEHNAKRALHGVASLSWDDTLASYAQNYADSYDCSGTLTHSGGQYGENLALGYTTTGSVDAWYSEGNGYDYGSGCSVYDHFTQVIWESTTKVGCGFKQCDSYWGTYIICSYDPAGNFVGKCDSQVVPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNN2</accession>
    <name>A0A1E3NNN2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_10669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018815.1">
        <property type="nucleotide sequence ID" value="XM_019159705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNN2"/>
    <dbReference type="GeneID" id="30176392"/>
    <dbReference type="OrthoDB" id="3997675at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="132" mass="14928" checksum="1B1B78F2187A1D4F" modified="2017-01-18" version="1">MLRRDPTTIHLVAEDIKDIGQGANYGIIQHPNIMEAGSRGKLDLEAVNNDPDNQNWDSSLDESYLGSRSRERETKRDTERDRANLGSKEREQELSQGNINNSLLRTLADSEGPHDKEEQHRTSGVFSKPFPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNR7</accession>
    <name>A0A1E3NNR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA damage checkpoint control protein RAD17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15644</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018847.1">
        <property type="nucleotide sequence ID" value="XM_019160905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNR7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNR7"/>
    <dbReference type="GeneID" id="30177592"/>
    <dbReference type="OrthoDB" id="337581at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003021">
        <property type="entry name" value="Rad1_Rec1_Rad17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02144">
        <property type="entry name" value="Rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="589"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="615"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="74572" checksum="D54288189A501B78" modified="2017-01-18" version="1">MTGNENVYESISANANDNRLGGLTQDIVQNKRRDIPVQQTKKNELLPQFESKTRNIQLLHQLISSVQSFGDFCLLDISSDGMCFSINDGNVCKVRLNLNRKVFQTYTFNGVWREKNNFSAAQLYAEDTDFSDAEAEEDGTENYVTNSFDCDAIISVNLNIASFLETMNIHVKDRKDAEQRVECTLRYDREGDPFVMVFEDDSIIERCELNTYYIDDLRSKGKKLKKKNRKSKEKKNTTDNIDVIESFDIDNPIIDDSIFRLDSNKILYDIIIKSNILHDIIKDMNDLNTDKFVLYCHKTKGHVANGMNDGNDEKEHRSKLIFISKSKSDTIGFSKLIIPQRKLNIPEFKLFKPVIPETNPEEYEFVDCYDLSLSSTYHFEYFSRLLKAIRLSKLIKIRKDMDGITSLLLLLGKSTHTLNQQQAQRLDNNDLYGSSIEFVTLESISIDELSALTPETRSPTLLSKLGYGNNFVEQLIKDDQDVQTIRVGNDGQLITLDDYFGKDARAEFEEIENVNIHDRDHEGYRGLPMEMDIVGDYMPVEGVHADKTKNRRQDDNILQLTEQLTMSLLGHATPTAINNIEVEQSMVVNDEVESEGDRTRKRLSNYRKEKDTNKRTKNRKTKGRPKKGANGKKKNDGIETVGGAIEIPLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNT9</accession>
    <name>A0A1E3NNT9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WKF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_23558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018855.1">
        <property type="nucleotide sequence ID" value="XM_019162312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNT9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNT9"/>
    <dbReference type="GeneID" id="30178999"/>
    <dbReference type="OrthoDB" id="10261563at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="2">
        <location>
            <begin position="81"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="219" mass="24893" checksum="AA5A3F13B25A14B0" modified="2017-01-18" version="1" fragment="single">PAWKRLGLKVKAVVGKDPLAIVTQREEGEDKTERKDKKQGKRKAEDGARARPAKAAKRVKLPRQERLQKQRQSAEEKDQLQYLRQFDEDREHWKFSKQKQNWLVKNIRHVPESCEGALLRYLESVQGGSRQRVADAMAAVVQTWNTMVDEAEDQMRRDLAAAAAETPDKPAGANGSSGDKPDKPKKQPDHASETPPDYDYAVRAREVLRVLTGETLFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNU6</accession>
    <name>A0A1E3NNU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71796</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018877.1">
        <property type="nucleotide sequence ID" value="XM_019164009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNU6"/>
    <dbReference type="GeneID" id="30180696"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="57" mass="6338" checksum="6FFD72DF5BE84509" modified="2017-01-18" version="1">MTSANLSSFRLLDALSLQGSFLMPYMFDIPARPTNLLNLILAKLLVNIVSNHIFSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP25</accession>
    <name>A0A1E3NP25_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018952.1">
        <property type="nucleotide sequence ID" value="XM_019160938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP25"/>
    <dbReference type="GeneID" id="30177625"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="73" mass="8506" checksum="25C774685EC2BCCD" modified="2017-01-18" version="1">MSPSCFCHLAVFLLLLLLVVEFCLFLKKAERSVRQRKIPRTHQHLSIIHRISTKQHSKFAFQPTNLPSCLFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP31</accession>
    <name>A0A1E3NP31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="7">PPIase</shortName>
            <ecNumber evidence="2 7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_58225</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 7"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018973.1">
        <property type="nucleotide sequence ID" value="XM_019163287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP31"/>
    <dbReference type="STRING" id="763406.A0A1E3NP31"/>
    <dbReference type="GeneID" id="30179974"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045835">
        <property type="term" value="P:negative regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045836">
        <property type="term" value="P:positive regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000013">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="161" mass="17351" checksum="4C8E2A9CD3ECD1FB" modified="2017-01-18" version="1">MSTTFFDVSANGQPLGRITFKLYSDVPKTAENFRALCTGEKGFGYKDSIFHRVIPDFMLQGGDFTNFNGTGGKSIYGAKFADENFVHKHTKPGLLSMANAGPNTNGSQFFITTVACPWLDGKHVVFGEVVDGLDVVRKVESLGSNSGATKAEIKITNSGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPF0</accession>
    <name>A0A1E3NPF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_22966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019097.1">
        <property type="nucleotide sequence ID" value="XM_019162288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPF0"/>
    <dbReference type="GeneID" id="30178975"/>
    <dbReference type="OrthoDB" id="10265837at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="10"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="116" mass="12710" checksum="0E5C5A06E9459B85" modified="2017-01-18" version="1" fragment="single">SSLEKLAKALQTNQSLQKETHEKLNADVVFEISGKGLKTPVVWLLRSKDAGGVQLGTAEGERPPADITIRVDDVNLRKLIHGKQSAQKLFMTGKLKIKGNVMKASYIEKLLKFAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPG9</accession>
    <name>A0A1E3NPG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_116427</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018721.1">
        <property type="nucleotide sequence ID" value="XM_019159819.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NPG9"/>
    <dbReference type="GeneID" id="30176506"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF113">
        <property type="entry name" value="PROTEIN JUMONJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="10">
        <location>
            <begin position="342"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="9">
        <location>
            <begin position="407"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="11">
        <location>
            <begin position="683"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="1468"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1665" mass="190679" checksum="25C1128550D60E9B" modified="2017-01-18" version="1">MSEDHIHFSSSVDDDFSKIGGPFSSNSQMLSSSKEQQIIRDIDDQAIKANDAIHNANSSFVLPPKIISSFTSPSSPMEVTEKNYDLPSQSSNDCLLTATNDVNQLNQLLSMNKNTSKRAFNDSDSADHIKRQQVDSVVSVDTDIPISFDTNELKLKDGLEDVSHDQNSYDLNSTNFSIKFEGLDSVDTPNSMTSDSVMTETANGSPSSFHRNGSDLAKSKVIKFTPNRTTENIKKPHLLLTNPNSKKSKREPTKGPPKNSRFFKVYSLDGSKTSKPSSIHHYNPYRYPQNRSKLNPHPDFIRNKKDDPITGPIKVPDVSSTETVQALANEVIDSVSKYENKIPELKPSIEEFEDIYSYITSIKEIGEKYGAVKIVPPKEFSPKFAINLESFWIKSKRQLWRSPSEELNARCEFYKQLKDCLKETGSISINKLPCIDKRAIDLYRLYRVVKLRGGFENCCNDKLWAQIGRELGFYGKISSSLSSSIKSVYQKYITPWEISNKDKNQDFLNLARNDEKNSLPLAPGTISAPNIPVILGSSAPMLRNRNTLINAGFSTYYDQATTQKKGVTLNDLQTLPDFDFYHWSDPLSVDDTNPTELKISSLYTLKQFYDKSRILKSQVLNKFFESEHYKFENLDYLESTFWKLLENPDVMFETEVAMGQSTNIHDSSYENRFLSEKNGDLSDSILGSLNFNNTTVADGSVLQYVNCDSDSIYHSSLNFSMFYGTQSWCLEDHWFYNIDYHYLGDAKSVYVIPPEYQEQYESLIKSQLQARSKEGEKTAETYKLFEKEIANYDIYTACLENQVCYDMNLPRSRPNDLGFEPLIENNSTPTRFNGDIMFSPEYLKENGIPVYHAYQEVGDIIIKFPKAYTSYFSLGTSVTESVNIANTDWLKESLNVSKWLQKQQIPPRFSTFAMLLTASRESKDIKLLSGVKDVLDHLVNDEIRKRNEIRMFLKEIKFSADCNVINHLNEIKDQENDTGISQDMAENDDETVGTKLAFKLGNWNKVTDYDLVDMFPTFVCAKNIANKQSNFTMSLDAFLTSIKSDDSKKYAYEMISLVDDEYLYETLDILKGKLQGMKEWSEKYHKVLDEYSVPPLEKILPLFEEGETIFDQKNFKFGQLDETERRSYEEFVYLRSEVKRTEEWRKKVNYFLGLKNQGVGELLPFSDFKKMAEDASLLTLSTKEATDVINLANEVVKFERLVAEPLSKGKDQLDLDELKKLHIIGSEIRVELESFKVLDKVVKRNSWIDSVQNKEINNLEGLKGVIEEGKKLSPSNEGDVKLLQDLEDKYKKANEINEKYQELKQSQRKISIDELKKLHEESKDAPLHEIREFTGRMIKEHDFITVNISPFIKLLREKDNVLAAEKELPEKVEVFLRYHEQINKSMSVENIENSIWGLTNYEDLEDDGNFLKQQIIEGPEEITREINENFQSLLLFGKDDFNFSNMNKSDCLNYLASYNMDLLNSNEDRYCVCRQMHEGNMVECENCKQWFHFNCIGYVSNGGQDDSSKYVCPLCDYENKYPTTHKFYADVSAKIKFEQLLEFATRMMKECSILTDYEVTFLRVVNKFYGFYRQVVSSGVVKLVRPADGGPEQLVVVENDVGTLRRLLQKIGACTINYDGLHRMLREQYCQLCVAARGGAEDGPAATPTAGLAHLQQHAGPSPEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPH1</accession>
    <name>A0A1E3NPH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-lactate dehydratase</fullName>
            <ecNumber evidence="1">4.2.1.130</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_71988</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>methylglyoxal + H2O = (R)-lactate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27754"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="EC" id="4.2.1.130"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="4.2.1.130" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019102.1">
        <property type="nucleotide sequence ID" value="XM_019164066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPH1"/>
    <dbReference type="GeneID" id="30180753"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019172">
        <property type="term" value="F:glyoxalase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036524">
        <property type="term" value="F:protein deglycase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017283">
        <property type="entry name" value="HchA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003168">
        <property type="entry name" value="PRK04155.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF20">
        <property type="entry name" value="PROTEIN_NUCLEIC ACID DEGLYCASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037798">
        <property type="entry name" value="Chaperone_HchA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="8">
        <location>
            <begin position="77"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="291" mass="32055" checksum="75BA8ABD2C391A73" modified="2017-01-18" version="1">MSSTELDHHPAPDTAEKNAFFPSPYSLSIYTSKKTDYDGTTYTHPYTGHKKILMIATDERYLKMQNGKYFSTGNHPVEMLLPVFHLDNAGFEIDVATLSGSPAKLEMWAMPTEDKAVMDTFKKYESKLENPFKLDDIVDKVCGPDSPYAGVFVPGGHGALMGIPESKSVKKVLDWVLEDNKYLITLCHGPACLLAAGIGEKPENFPFKGYSVCVFPDSLDSGANQEIGYMPGQLNWLLGDRLEKLGVKVLNKDITGKCHKDRKLLTGDSPLASNNLGKLAAETLLADPEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPL4</accession>
    <name>A0A1E3NPL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone chaperone ASF1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Anti-silencing function protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Histone chaperone asf1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_58357</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ASF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019152.1">
        <property type="nucleotide sequence ID" value="XM_019163293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPL4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPL4"/>
    <dbReference type="GeneID" id="30179980"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1490:FF:000001">
        <property type="entry name" value="Histone chaperone ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="448"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="849"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ48039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="922" mass="105790" checksum="A0753193EBEDF0FA" modified="2017-01-18" version="1">MSIVSLLGIKVLNNPAKFTDPYEFEITFECLEQLKDDLEWKLTYVGSSRSLEHDQELDSILVGPIPVGINKFILTADAPSPELIPASELVSVTVILLSCSYKEREFVRVGYYVNNEYDSEELRENPPTKVQVDHIVRNILAEKPRVTRFNIVWDNEGEMDEYPPEQGEGEDENGEAVIDMGANEDEDEEDADEEEVEEEEEEDGEDDELYPDFIYPNTNPISEDATQLKYAYRSTGEVFGQLVEEVHHDRDPLHNSRVITSEEALEGVDPRYRDPQTGQLLNAATSKEARLDSETLWGRVNQKQIRIPDALADVIQNNLLFQYEPKAFKEHVVQWYTSINESGLRQECKSELDADVSIAASFAQDYAVSYQVLDEYVKRLGKEKTSKINSVLDIGVGPSVSMLAFNEIMGDTWNPERKDSVVYGNFHMARRAKLLLSRQINENFSREKELEEFQEDSGENAEKEEPLKESININNSNGPLNEYGEEEVFVGKVKTKSLKVKTVLLDRLRPKDIKYDLIVVSHQLLKSMEYFPFEVDSLLEEYVRRLNPGGHLVLIERGTPLGSEIIARARQVILRPENFPDELQKYPRPFKSSAKKMSAKEQLRLSKLSPEEKALAMKNLEPELLKNFEVVEDEEIAEGLDALDEPIDMSIIAPCSHHGKCPLQFFDPQVYLYGQIGKKLKFCSYNVSVHRPNYLLELKRGKKLAVKWTAANSGVGISSLARAGRGRENGNDYETASYSYLITERSVEDGPSLEKERVKRAEDGFEKREIGYRAQNRGEYPRILSAPMKKKGYVIMDLCAPSGHLEKWHVSKSVGKQEYHDARKSEMGDLWALGYKSANQSRKENTFYFERIEEKRENLRKKKKKDATRLKRKISKDYKEALESENEDLDEDVVRMAKIDAYEFLTKPKETEKLKDKSKYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPM6</accession>
    <name>A0A1E3NPM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS19m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_32000</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019089.1">
        <property type="nucleotide sequence ID" value="XM_019162737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPM6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPM6"/>
    <dbReference type="GeneID" id="30179424"/>
    <dbReference type="OrthoDB" id="2043at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF8">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="91" mass="10262" checksum="F9632C6C3C7F7ED2" modified="2017-01-18" version="1">MRASLVALGRSSWKGPFVVPLPIAQAKKNGTPIRTNARSCTIMPQFVGLKFQVHNGRDYVQVEITENMVGSKLGEFAHTRKRWSYTQTKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPU0</accession>
    <name>A0A1E3NPU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ribonuclease T2-like</fullName>
            <ecNumber evidence="4">4.6.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_15917</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Rnase which modulates cell survival under stress conditions. Released from the vacuole to the cytoplasm during stress to promote tRNA and rRNA cleavage and to activate separately a downstream pathway that promotes cell death. Involved in cell size, vacuolar morphology and growth at high temperatures and high salt concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the RNase T2 family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.19" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019188.1">
        <property type="nucleotide sequence ID" value="XM_019161021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPU0"/>
    <dbReference type="GeneID" id="30177708"/>
    <dbReference type="OrthoDB" id="435754at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000004">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057328">
        <property type="entry name" value="RNaseT2L_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25488">
        <property type="entry name" value="RNaseT2L_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="RNase T2-like C-terminal" evidence="18">
        <location>
            <begin position="303"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ48075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="411" mass="46090" checksum="3B8C5092217D3990" modified="2017-01-18" version="1">MLSLLLTVLTANAVSIPDAFNPQNALKGLKDFTENWTNEVKKCPADIPFTCTNDTVISDSCCFEYPGGVLLQTQFWDYYPAVGPDDMFTLHGLWPDRCDGQYDQFCDPSREIHSAKSILESFGETELLNKMSRVWKNFNGNDESLWVHEFNKHGTCMTTIKESCYSADTKPNQNIVDFYKKSVELFEGLPTYEWLAANGIVPSNEQTYTKQQIEDTLRARFGQPVFIKCNRYQAIQEVWYFHHLRGSIVDGFYQPIAALLESQCPENGIKFIPKKGFAPPKPTHTDPSSPVPTGKVLHGYLKPENQPGCVISNGKWYSSGTCAKFTLSKAEFGGYNIKSSKGYCKIGTEGFLLCGAGVSPMQFAYDREKGLVTFGGKNKWSADKVPQRFTQIPISPGYDGPITFHLKLVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPW9</accession>
    <name>A0A1E3NPW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15967</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA1 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019260.1">
        <property type="nucleotide sequence ID" value="XM_019161038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPW9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPW9"/>
    <dbReference type="GeneID" id="30177725"/>
    <dbReference type="OrthoDB" id="1920692at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017384">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17098:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17098">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MWFE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="147" mass="17051" checksum="27A129F9B6E07C09" modified="2017-01-18" version="1">MIPWEGLIPYGLILSFFALAGGGMNAITISSLTRNAQGPNHESNYSVPTEEKDWVRPRTKTDQWDKYLAVRDLRLTGNFRGQKAEPEAEEAFETNSVVFASNTLKPWVLRKHIIMKNSPSKWMKKYNSEREQQWEAVKENYLRSMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX3</accession>
    <name>A0A1E3NPX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-terminal of MaoC-like dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018706.1">
        <property type="nucleotide sequence ID" value="XM_019163972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPX3"/>
    <dbReference type="GeneID" id="30180659"/>
    <dbReference type="OrthoDB" id="3257538at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052741">
        <property type="entry name" value="Mitochondrial_HTD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152:SF1">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39855" checksum="9172DE21EE04E227" modified="2017-01-18" version="1">MKSIKTLSNSKAMHIDMNRVRRFSKFVKATSIPTEEEVSRIRSRLLKSSWHLFDDVISTYPAKQLYVTLESLLNKSNFPFMESLNSDNDVYRENFDLNKQFKIGDNLPIGYSLAYCNPLSSEQELSPDGYDNYHAPTTNSKEFFKRRMWVSGSFNYDKSNSLRFGDTIHFTETVERVKFLPKNNVIFADYKRCFDNTHGNSLIEFRRLCYLDKKFETTEKESKTSSIVPDKSIVLHPSIITSFRMSALTFNSHQIHYNPQYAQNIENYTDVVLEAPLLVALVLQFWSDNNPGTMISSFKYKITSPCFINAPMTINYKMCDDHTQLWICNDKSRTCFESTIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ94</accession>
    <name>A0A1E3NQ94_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_23152</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018826.1">
        <property type="nucleotide sequence ID" value="XM_019162298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ94"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ94"/>
    <dbReference type="GeneID" id="30178985"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133479" description="Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase" evidence="15">
        <location>
            <begin position="20"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="212" mass="23513" checksum="3B915908AA6B1D8F" modified="2017-01-18" version="1" fragment="single">YNLLSGCLWSFALLNVVATAGVFGDLRTVFKLTHVWTTAIQLLAVVEIYNSATGLVRSPLFTTVMQVTSRLVVVLGVWTLLPEAAGNASYAYLSVHVAWGLAEVVRYYYYATNLMSQQQGNPTTVPPTLEWLRYNAFIVLYPLGISSECYMIYYAVAAAAARDGVFFAAYALFLCLTLVLYVPGTVVLFSHMLKQRAKFNAQRRQAPAKKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQB0</accession>
    <name>A0A1E3NQB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">anthranilate synthase</fullName>
            <ecNumber evidence="3">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_72267</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019410.1">
        <property type="nucleotide sequence ID" value="XM_019164137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQB0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQB0"/>
    <dbReference type="GeneID" id="30180824"/>
    <dbReference type="OrthoDB" id="1865897at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005950">
        <property type="term" value="C:anthranilate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005256">
        <property type="entry name" value="Anth_synth_I_PabB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00564">
        <property type="entry name" value="trpE_most"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="10">
        <location>
            <begin position="35"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="9">
        <location>
            <begin position="227"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="498" mass="56084" checksum="D1EE52118E5BA845" modified="2017-01-18" version="1">MTESQPSFRQLSDYIANDQESNVFPVYKFLSAHYLTPHTAYLKLVGAQSTPAASFLFESATDGEQVSRYSFIGASPRSIIKTGPTESNEIDPLILLEEKLAGIRQAQLPGVPKLSGGAIGYVSYDCIKYFEPTTKNDNLKDTLKLPESFLMLFDLIVAYDSVFQRFQIINNIHVDRKVDDEKSLQEKYDQALKEIEDVAKILESPEVKYPVQPKIKDPETFTSNIGQEGYENFVHELKEHIQKGDIIQAVPSQRISRPTKLHPFNIYNHLRTVNPSPYMFYIDYLDFQIVGASPELLVKSDSNDKVITHPIAGTIRRGKTIEEDNKLANELKSSLKDRAEHVMLVDLARNDINRVCDPKSTNVDSLLQVQRFSHVMHLVSQVSGTLRPEKSRFDAFRSIFPAGTVSGAPKVRAMQLIGELEKEKRGVYAGAVGHWSYDGKTMDTCIALRTMVVKDGVAYLQAGGGIVFDSDPYDEYIETMNKMKANNNTIVEAEKMYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQK8</accession>
    <name>A0A1E3NQK8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_29750</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019484.1">
        <property type="nucleotide sequence ID" value="XM_019162607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQK8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQK8"/>
    <dbReference type="GeneID" id="30179294"/>
    <dbReference type="OrthoDB" id="248120at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23161">
        <property type="entry name" value="Prefoldin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000003">
        <property type="entry name" value="Prefoldin subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11868" checksum="6F21C758C3ED4C8F" modified="2017-01-18" version="1">MEELTQLQRALQGLIQSRTRLETQFQENKIVKKEFESLSSDSSVYKLVGPVLLKQDKEEAEDNVGKRLEFITSEIEKVEKNIKATQSTMQSKRLALQQQQQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQX8</accession>
    <name>A0A1E3NQX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_14061</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CKS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019614.1">
        <property type="nucleotide sequence ID" value="XM_019160203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQX8"/>
    <dbReference type="GeneID" id="30176890"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00944">
        <property type="entry name" value="CKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="147" mass="17274" checksum="066DC4EB09118F8C" modified="2017-01-18" version="1">MSSFNQEDHAAKVQAQAQAQAQAQAQKQQSHTQNHGHPHAHHNAHVLQLQRPKPLTQQERERIFAYRDKYYYSPRYSDDEYEYRHVALPRDMLKVIPSDYFSDKGTFKILAEEEWRTQLGITQSLGWVHYGCHAPEPHILLFRKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQY2</accession>
    <name>A0A1E3NQY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_70108</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019588.1">
        <property type="nucleotide sequence ID" value="XM_019163554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQY2"/>
    <dbReference type="GeneID" id="30180241"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="10">
        <location>
            <begin position="24"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="12">
        <location>
            <begin position="256"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="11">
        <location>
            <begin position="320"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="473"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="487"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="519" mass="59246" checksum="11912B19D7B56447" modified="2017-01-18" version="1">MSNYSNIDTHSHGVAGQGQATVLCCNCGVPMDGSTGLVMCYDCIKLNVDITNGIPREANLSFCRNCERFLQPPTNWIKAELESRELLALCLRRLKGLNKVRLIDAAFIWTEPHSRRVRVKLTVQGEAFSNAIIQQSFEVEYVVIAMQCPDCAKSYTAHTWNTAVQIRQKVPHKRTFLYLEQLILKYNAHVDTISIKENKDGLDFYYAKQNHAVKMLDFLNSTVPMKSKKSEELITADIHSGTSVYKFSYSVEIAPICKDDLVILPAKLAKSMGNISRLVLCSKITNSIQFIDPANLHIEDMQATVYWRWGFQSLADVTQLVEFMVLDVEPTGERRGKYLMADVTCVRASDMGGSSNEEFYIRSHLGGLLHPGDSAMGYYLKNSNFNSDLFDELDADNVVDVVLVKKHYLRSNKRTKNRHWKLKRMANERKDIVATEVLDSRQAKQEQERAERDYELFLQELEEDEELRQNINLYKSDKVPKPVNPDDMVDEDADAEDDDAPQIDIDELLDELDEMNLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR27</accession>
    <name>A0A1E3NR27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019658.1">
        <property type="nucleotide sequence ID" value="XM_019163579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR27"/>
    <dbReference type="STRING" id="763406.A0A1E3NR27"/>
    <dbReference type="GeneID" id="30180266"/>
    <dbReference type="OrthoDB" id="1507364at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="356" mass="39691" checksum="8332D689A69406A6" modified="2017-01-18" version="1">MSDCTPEQKKLVEEILTIKHTDYYRVLKVDKSANEIEIKKSYRKLAIKLHPDKNKHPKASEAFKVIAKAFEILGDDSKRKIYDMTGSDPDSRGGMGGGTGMGGGMGNMGGFPGFQGFQGFPQAAAGGGGPLNEDIFNMLFGMGGMGGNGFSFQFGGNGFPNNGYYYTNNAGMNARRHAAQQQHQQRQNARRGANGNGTPSELPWSQYIIQFLPLLIILISIIINSLSGGSGSNDGYRNVREFSGRVPKYSFESSGLLSEKRTTPKYNLNYYLEKKTLDNFKGRKNPAKELNGLDKFVENQYVDRVSLGCLRERRTKESIIEQAQGIFFNDWERIDQANNMAMPNCERLEELQKRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR30</accession>
    <name>A0A1E3NR30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_9078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019655.1">
        <property type="nucleotide sequence ID" value="XM_019165061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR30"/>
    <dbReference type="STRING" id="763406.A0A1E3NR30"/>
    <dbReference type="GeneID" id="30181748"/>
    <dbReference type="OrthoDB" id="10501715at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="552" mass="60537" checksum="F263E2C2D47D532C" modified="2017-01-18" version="1">MNTGDGEKTQLDPLPVKGQIEQCTKGQNWSASLEESPFEYQCAEIDHIVSVRAAIKEGLDLKHGGSINSWESSNYNNNTNYNSGSNYSLNYRNYEYNNGHTNITGNSSNYGVNYDNGNMGSGNHLMYKYNNNNSNNYNNRQNNRQNSKGNYKNKNKRMNQAQNSNQTLNQVNNNANSTNYNGQRRYKNFNSFPDQQQLDQQNMLYNSMVGMNQTMDTSVLNGGLSLGASPGNLLQQLQQQQQQQQQLHFMNKGLQNPQVEAGTQEQGQNTHLLESFLFQQLSSQNQVQSPSCKHVQNPLETQLQSLGNTGGSMDSIFARGTGFSNISNNEISNTSSLLPKLNTTSQQQQPIHRTTTDSHLTSYSNSTSNSNSVSAPNSASNNESSSSSSNYSFQPSQLKPFKFDSLSPFNFQSLMDQPEISTPGSTITRAGSSLASLGSSIPSSSTNRENSLSFMSDMLDSNGTSTNMNLSNLSNTTGLSSTMTMVDPLESIWKRGHNTNSTISNSSNLVSTPGENGSKASDDFGMGTYVGNLNVADGNRLEDIGLFGSYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR40</accession>
    <name>A0A1E3NR40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019665.1">
        <property type="nucleotide sequence ID" value="XM_019163580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR40"/>
    <dbReference type="GeneID" id="30180267"/>
    <dbReference type="OrthoDB" id="5376259at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021750">
        <property type="entry name" value="Sid4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11778">
        <property type="entry name" value="SID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="582" mass="67173" checksum="26A079CCD0FAC3C7" modified="2017-01-18" version="1">MYTGNNRRHEENWHYDKYKDPHEFPILDVSSDEERSEYDSHTAESSANLSKKLPQISDEKFKQILELRKQSKKRGRNLMLSNKINDLPQPAVSDKLPIHEDGKANEPALRNATTDENLLSASTPKVNNTVTFNEESSSSKTNKSNKNISHLLLQQENEAASPNDKNSTESGIEKLIETLVEQMQQINNLEVQLNKSKIQVVNLGSKIVVLENENSKLAGKAEQWEAENKEARNYQQSLMQEIAYLQEEMAESENKYKYSFNKSAKLDSDLKKEKLLLKKERDMHKKIIQNYDSKFESLHGEKMQALDLVEEEKNETAFWKKKSGMFEDECKRLEKKGKQLEEEQENKIGSLETKIEQITSECELLRVENDKLARYEEDYKSLNQEHEHLKKSKDNLQSKVDELSEKNTTLSTGNVTLKAELTTKLEIVQNELVKVKEENYELTISNQSLKSEIHILNEKHEDFTKKMLGASPITDEQFGARYKSLEMEQVDQLTLMELQNIVKNILSSLNIKYSGLKGHIVFIRDHIFVFFNEMHSILHSEKRGNSVIIDRSIKLDTSDEAKMKACMNLLIQDIKQLKGLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR79</accession>
    <name>A0A1E3NR79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWR1-complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_30079</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019699.1">
        <property type="nucleotide sequence ID" value="XM_019162625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR79"/>
    <dbReference type="STRING" id="763406.A0A1E3NR79"/>
    <dbReference type="GeneID" id="30179312"/>
    <dbReference type="OrthoDB" id="19740at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032563">
        <property type="entry name" value="DAMP1_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027109">
        <property type="entry name" value="Swc4/Dmap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855:SF10">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16282">
        <property type="entry name" value="SANT_DAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DAMP1 SANT/Myb-like" evidence="10">
        <location>
            <begin position="184"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="12"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="369"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="514" mass="57911" checksum="12467874A86B1293" modified="2017-01-18" version="1">MSDILDVLNIKSFPQKKGKEGNQQPQQTSSESRQIYTPDDYATSTKRRKLSAMNRELYNLIGSNVPSLAIDTASVANAGSGKKFKEKLVNTKKNAPWKHIGFKNSARPDGLVLYHWTKSDNEIELDPNEYRYAKYNTSLDIPTFTEEEYEEAMKEDEEKVEETIQKEDSENVEGAASQDLEVGERTWTYDETKFLFDLCSTFDMRWAVIYDRYQDKYTGRTIEDLKVQMYTISAHLLEKRGNLNDSALIKALRSFNKDKELERKYYLTKLIHRAPTEIAEEESLVIEARKFELAAKKMLLERAQLLQLLDSPQSSASVQQYLTSQGLTQLYNSLMAADKSKKRKAEVPVAPLLGPNAIPHTQHLQIGNKRRSNAGQNSVGSNGGAGNGKATVSSSSSNGVKPPSKLSEVQQLLQKNLTAEELEVYGIEIHNERIQPGVHVRSQKIASFKPSVQAKVHEILGQLGLSTKPTMPTGPVSKKFDDLLYKISHLVDLKKQSDKLLAEIELIKRQKGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRE4</accession>
    <name>A0A1E3NRE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72229</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019371.1">
        <property type="nucleotide sequence ID" value="XM_019164131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRE4"/>
    <dbReference type="STRING" id="763406.A0A1E3NRE4"/>
    <dbReference type="GeneID" id="30180818"/>
    <dbReference type="OrthoDB" id="427368at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013211">
        <property type="entry name" value="LVIVD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022100">
        <property type="entry name" value="WDHD1/CFT4_beta-prop_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048591">
        <property type="entry name" value="WDHD1/CFT4_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932:SF10">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20946">
        <property type="entry name" value="Ctf4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08309">
        <property type="entry name" value="LVIVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12341">
        <property type="entry name" value="Mcl1_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="297"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 second beta-propeller" evidence="7">
        <location>
            <begin position="496"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 helical bundle" evidence="8">
        <location>
            <begin position="837"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="385"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="797"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="441"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="799"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="810"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="954" mass="106565" checksum="14922B149E73591A" modified="2017-01-18" version="1">MHLDDKLTPAYFQETAVQVASTSNTLFTASNLSIKVFNLKNSSSEPEIVNTLDQLKGIDIKVFNSKTSALVAGGFDCAWHDLSDFIVKDNGDNAHETFGISEMKKLWKSSSRITQAIFTHGGQRACVGDLAANMVILDVSNPKDETTFGRVVEKVKVSGEITGLAYSYSVDLLAVTVADGGLTIYSLSSNTPEKKATIGTILADKRIALTHEMGLVDDDSDMSDFSDEDDDSMVTESTLGGGKKQRDSKNNLLKFAASKTVWNPVGDILAVPNKNFKIDFYNVVNWSAPTFCLSAGPLHHKDYVSALGFSPDGKYFASVSLDKLLLIWDLATKKVIGRTNIPIIGCDITWPDDSSIIVGSSKGQILTIDKDTFKTLVGQRPIRETNDEIEEKVNGKITAEDSDDMGDDVLQSNDEENEEDVNLDRPTSQDLDGFVIDDDDNQRYYGEEDSNRRNHETMSESTELTDFNKRARKEKAGDSHLYRLSVKSGWGRSLTKPFSVGSTPWAGSDRRYLAITSVGCAYTVKTQDFFKITVLFFDEENHRKYFFDDLVGYDICSINDQGSVFATSGFKKSKGFTARIEYKDHSNGRGGETQWIREVTMRPYEFITSLSVYGNFVFVCTNKGFVRKFNLYGRLLNMSMMDCVVGIMNNNYYVFTIIKRSIESGYVFNIQNLDGDYIQQEVGIPLITENDLNDDAADVPIKSMFFSAEGEPCVIRNDNLLLVLTKWRDPSNGPIWKPLLDIQEGVSRAGRGRELKAWPLGLYGDSFTFVPFRGSKNYPIFPLLTPMNVDIRIPLSYEDPDKKPRQRREEEEDAGLFGDENENEGPTTNGDTEKPENPEEKFLRTLVLAEMLNDAISNNDATDSDTIEKLQLLSLEYDKTLLIQIDDACSSGDSDDAFELCKMIRDFRALNAAQRLAEVKGMAGLARRVRDLREARMAEEEEEAVEEVNSDADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRE9</accession>
    <name>A0A1E3NRE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Copper-fist domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_9184</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019769.1">
        <property type="nucleotide sequence ID" value="XM_019165074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRE9"/>
    <dbReference type="GeneID" id="30181761"/>
    <dbReference type="OrthoDB" id="5600085at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.430.10:FF:000001">
        <property type="entry name" value="Copper fist DNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051763">
        <property type="entry name" value="Copper_Homeo_Regul"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00617">
        <property type="entry name" value="COPPERFIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01119">
        <property type="entry name" value="COPPER_FIST_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Copper-fist" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="634" mass="70405" checksum="88C9C7ABC0F6B690" modified="2017-01-18" version="1">MILVDGEKYACQQCIRGHRSSTCKHIKRPLVLVRSRGRPLTDSFQRIAIFAEEIQDEDEKKKVLDKEKNPGNNLLAAKRVEKREASCCHKPNSKSSSTQSCCASKEPVNLKEVKKVSENPSVKTNCSCCATGAASSSCKKHSSPVYVLKASKRQVYNVEKDSLRLLDPVVEIPNSKVGLDIIQKVSKNKKMYSCRDKRIKEELIRSVVYPTKHTPNSSCCSNKNITELKQLNLGKDGNSADTNNTPFVYQFQLSMNKPNKLSIDKKSANRITPEVNIIDPNFSSLKQKVDVDDLGQFSPGSSEFEYPSNIIRSSANAFEFVSAPSYSSTSNNGSTEMPNSTLPDISNSLLYDLYIAESCTVPGSCSCEAESCACPDCTEHGKYRNSSLTVKQQFEEYPFPINDAPSSADNTSHKEIHGIRPYGKVEMQPSSVPLFEQTFLKVLGSQVIEESRQNPLGIPEENNSPNTEMDECYCEPDQCCCYNCIEHGIINGIRISDGVCIAEGERSSPQEIQLLASQFPLYANSPTSSSRSTPAVMSASESEEYLKNPFPSNLMEARINASNNSADSNHLVKESVPDREQWMRYHNNMYACHIDNAHDFIQEGNGYKTLNPSTDGHPASVNSQDETIRTLLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRF9</accession>
    <name>A0A1E3NRF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATPase expression protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019783.1">
        <property type="nucleotide sequence ID" value="XM_019160269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRF9"/>
    <dbReference type="GeneID" id="30176956"/>
    <dbReference type="OrthoDB" id="3992587at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="56506" checksum="45440554ED08354A" modified="2017-01-18" version="1">MTTKRISLFGKLKNGAFSNRLMRNERFNSTIATKAMDDFIDDPEVAKRKSHIKLHLTGKPFDGVDDYSTPNHYAIHTVDKLNPDIIPSPFVYLDETAALNMILNQSSQSKDLGKYDYFLRMKWDNVGNFDDKIDIILNWQKRFKKDYKSISPSDSSIKLKFSDDLRRAMLDAIADEANFDLKVALDSLSDSFPKYSFESLALLFIKIIPELPASKQKAFINSMNDFFPQNILHFHDQVLESICFELVIQKKVQSVTTIFRAYNEFSHSEPELLKVVSKSFCELYLKGLLEVRDVQTARQVLKTIVDANYIPSADILTNYFELVSDVCNQANAPKDKKEMLFNIFTQPVSSVILQEGMLNKRILRTISSFIRLAVLPQFIDYLKLSSNFKSMSYIPDVIIERIMGSHTYSIQSDQQKAIFLSSILKLLDLDMSSLADVTKKKIIKLYAESHSPLAVLFWNKNLKEPLSEIEKSDFCGALQGVTNNVSGSYDISSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRP1</accession>
    <name>A0A1E3NRP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14291</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019878.1">
        <property type="nucleotide sequence ID" value="XM_019160311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRP1"/>
    <dbReference type="GeneID" id="30176998"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046964">
        <property type="term" value="F:3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902557">
        <property type="term" value="F:5'-adenylyl sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="17"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="122"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="238"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="35209" checksum="02874FC814071900" modified="2017-01-18" version="1">MTEHGDKSQAPWKVLQRDPCLSFICGGAAGALSRTAVSPIERVKVLFQVQGGGGGNGGGGVSYGKGTLATVAQIYREEGYRGLFRGNGVNCVRIVPYTAIQYTVYEDLKDMVTGGVRPLATGEKLACGMAGGLASVLGTYPMDLVKTRLSVLTARSLKGVGGAGAAAGTAAAVDLSMWGNLRSIYLHEGGLRGLYRGFVPTSLGVAPYVALNFALFERMKEAVAQTWPATHKSQSPAAETAVVLLVGALSGGIAQSLVYPFDILRRRFQVATMNDGSLGFSYTGTWDALTHIVRREGVRGLYKGWAANMFKIMPSMAVQWASYDLIRRLVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRY4</accession>
    <name>A0A1E3NRY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal recognition particle receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_80042</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SRP receptor beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019436.1">
        <property type="nucleotide sequence ID" value="XM_019164979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRY4"/>
    <dbReference type="STRING" id="763406.A0A1E3NRY4"/>
    <dbReference type="GeneID" id="30181666"/>
    <dbReference type="OrthoDB" id="41266at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019009">
        <property type="entry name" value="SRP_receptor_beta_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09439">
        <property type="entry name" value="SRPRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="279" mass="31128" checksum="6EC653D3271F728F" modified="2017-01-18" version="1">MEALKEEIKAEAYAIESNVETLVETTPTSSLILPILVTLLLILIGGFLMKRHSLISSNKRSFVICGLQGSGKTNLFHLLTKGKLPVLTVSTLEPGKGELKLSEEYSNQKTFQDIEILDFPANPKLKTLYLTPHLHANLNSVQGIIYIIDSSNFDTKSCHQAAEDLLELMNITEGKPNGVDILVFANKNDLFTSKKSSKIKEMLEAEVSKIYQLKLRGLSKVDLAGKDGGEEDNDNLDLATKDGKFEFQQLEGNVDFAQGNIFKDKWSMIVDWMYEKIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSF1</accession>
    <name>A0A1E3NSF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_9446</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020049.1">
        <property type="nucleotide sequence ID" value="XM_019165094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSF1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSF1"/>
    <dbReference type="GeneID" id="30181781"/>
    <dbReference type="OrthoDB" id="2401875at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013256">
        <property type="entry name" value="Chromatin_SPT2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08243">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00784">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="450" mass="50098" checksum="6BD31790A69681C8" modified="2017-01-18" version="1">MSFASLLSQITKDKKLEEKARLSGLSNQSKASSSQAKSPAGSKASPAVAAHRTLQLGKTQPKTYFDDEDPAVRRLKEARRKEKEKLQERKAGASSSKSRSRTKSQPDSAARSRSMKPASASLLAKSAGQSSSASPPVSVATKSRFKNKPYLKSSTSPAADSGTAAPKLSFRELMKQAESIEKPVVASTPFKTAKKHIGNEPSKAYQSLQKSRSRENEYLHSSALSSRRNGSRPPASPPPRASHKLDISTGPRSRPFKASSSASSNPQRRKPALAQPNPDLLSKLKKRQSQLQSKQSGQTYPSTRRKLSGSKSQDAYGIDAGDSDGSDDYGYGNGYDDYEDDGFIVDDEEEEEEGYGDNVEYHNRIRKEKKRRMESQGYNKEEIWEIFNRGKKRNYYDRDAYDSDDMEATGTEILEDEERTLKQAKLDDLREQKLLERKAAEKKRMLHKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSQ7</accession>
    <name>A0A1E3NSQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_14660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020300.1">
        <property type="nucleotide sequence ID" value="XM_019160476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSQ7"/>
    <dbReference type="GeneID" id="30177163"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000254">
        <property type="term" value="F:C-4 methylsterol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="144"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="307" mass="36371" checksum="6294B131BDFCFFA0" modified="2017-01-18" version="1">MTEIFNSTVLETIESPSLLTVVENVFHTQHQLNVVEKLWASWYAYMQNDTLATGLLFFLAHELFYFGRCLPWYVIDKIPYFRRWKIQPTYIPSDKEQWECMKSVLKSHFLVEVFPIWTFHPLCKSLGISVSVPFPPFTTMLKEWALFFVLEDMWHYWAHRLFHYGPFYRYVHKQHHKYAAPFGLTAEYAHPAEVISLGAGTVCFPILYAYIYRDLHLFTLTCWIILRLMQAVDSHSGYDFPWSLHNFVPFWAGAEHHDLHHHYFIGNYASSFRFWDYVLDTEAGPEAKAEREARRKAKAETAAKKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSR7</accession>
    <name>A0A1E3NSR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphodiesterase</fullName>
            <ecNumber evidence="6">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70674</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="6">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="6"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020213.1">
        <property type="nucleotide sequence ID" value="XM_019163703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSR7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSR7"/>
    <dbReference type="GeneID" id="30180390"/>
    <dbReference type="OrthoDB" id="546632at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="7">
        <location>
            <begin position="197"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="276"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="539" mass="61629" checksum="012CBB2528BBC3EE" modified="2017-01-18" version="1">MLNDLLLLGFTEQTLPESLANCYPHASTHIATFPNIKALVEYLLSSPNYSLVRNSTDHAQIERADTSLIEKEIDYSVTIVINSNPAYYAQQISDSSNTSLSSLSLKNLLSLLNYRFLHLNPIILSLNSSDENEDDITHVFSELSIVENNLSTRLARIKCWIGFNDRQFDQLDTMINYLNLDFPVDSDMALFTKLLSTDIDYDKLLINLESHKDDEYYLNLINKWEFNAFHFNLDELLKMSHVILKSYSINAQSHSQTLSSFLFFVRDNYRIGNPFHNFRHAIDVLQATNFFLNILTRDSSFKLSKSDSFALLLASLGHDIGHPGITNAFLINTKSPLAAYFDNHSILENFHRLQFKKILIPFLDQSKDLFPVDGNTHLKYTLSVVDNAILATDMAKHDDYVKEIYNLESDFDNFKLLSCILIKCADISNVCRPLNPSCKWGLSLGEEFKQISCLEAYFKSSTPSRDLLQDDLIANNFGKPIKDMDLHEGITLVDNLSNNQLFFINRFANDFFTKVANTIPQLHFLADHLIENAAFWESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT97</accession>
    <name>A0A1E3NT97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3020 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020349.1">
        <property type="nucleotide sequence ID" value="XM_019160490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT97"/>
    <dbReference type="STRING" id="763406.A0A1E3NT97"/>
    <dbReference type="GeneID" id="30177177"/>
    <dbReference type="OrthoDB" id="5595797at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021386">
        <property type="entry name" value="SPP41_DUF3020"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11223">
        <property type="entry name" value="DUF3020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3020" evidence="2">
        <location>
            <begin position="735"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1265" mass="141091" checksum="BC46E12B7D9DDEAC" modified="2017-01-18" version="1">MFDKLDAEHSQNNNDTTVDNGRHKSGELDEDAVLSLTNSIVQSLYPEEEGSEHNQNNIIDHNQQREVEGNSASNTVDKSDHNTNDEDIDYQFDQEALISNLAGSVVESLEHQMHDHYQDNSEHRDEEKTTDSQENEDDGQPMEREHIEHNHQLAFINKPAIPSDHHVTHEETEKDANLEKIIADTFRDQFQVDESHQLETEENEIQPEKDMMSLDLVNAINEAFQGNVLDEKVENADKNTNVSRPEDKHDNGLTEKVTHEQMQPVDDTKDVELGLDDAINYALNDASKTAGGMHQKKDSDEQGKLEHDEAEIDLNAAIEDALKSTQSVLESRDDRQVATTEDANHSFDNIYQDMQNLVESTVSEVEKDESEPLAANASEAKAEGMVNTDIERVIHGIDLNDVIATAMKDTQAKAPEYDVNVELNNAIEEALLSVPGTKAKKKPAKKAQSKNKATNGSSLEKKRNSDTNNEIKELNEDLAAELTSAITDALKDTQLTDQNKPVQAQQFNTTESEFEKALKSVVNDVVEHNLVGKKIEDATTDDHSKDDEDDEQKDDEFNEEKDGDYNWDDIMDKAFEMAMDYPSDLNLQVDEADLQRDHLVNKTNVPFTHSTSQTALRKPVSQSQPITFIQTANIEKLRSEFDKTNNIKRQKVKVVTPSIPPKSSVAAVVGDLLSSMNLSSIQKADILKLKLSELDAIKKNVSSTIATLMNSNHQTIKEVKSMEKMDEKEKIRYENRERKKRWREFNIERNRDIDLRTRVIKRANHLYPLPEHEDLRKQWVSSEFYKRKQKRIERQQKQRTNFIDMKNFDSKDALPFDLFFKDKENLQKIAEIYNEIGGDVSGEKLLLPSSDKIVSISSIVSALVAAYLLKNGGGKEEKIVQSITQSLVVSLDRYLTKISKDGNGIHEAKDALDSPTPLEWDNTTDSYDIPFDTGSAHGASNAQTTGKISLGLTDNTPRIAPASGGITFKVQSQNIENLQVERPTYTADADLTRLVKEKNIALKSTKLSESKAAAGVAAEKVSNVLKKIGESKPKRKAAAPVAINIEKFLSPQPPDASFMQKIKGDDKKRKHEEIAGKVTEELIVKRRVDDDKSAEGRKLDENVRSDQNIANGQLNLGSNKIDTVEATTFKERIEDVGNPDATLVEEVTPRVAPKQLVKPTSATSKTSSKIPTIPLPQYGTPSTSKAGMGSIRKVPVSLKKAGSSSHIWEEKLANTKKPTSIIGGIAGLKRPGAFRKPGAFRKPSDVGSSYGILKPFGGITPIKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTC7</accession>
    <name>A0A1E3NTC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020379.1">
        <property type="nucleotide sequence ID" value="XM_019160501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTC7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTC7"/>
    <dbReference type="GeneID" id="30177188"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043408">
        <property type="term" value="P:regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13279">
        <property type="entry name" value="PH_Cla4_Ste20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000139">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050285">
        <property type="entry name" value="STE20_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48015">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48015:SF6">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CLA4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="44"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="627"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="900" mass="100361" checksum="FC8F9BD491B71BB7" modified="2017-01-18" version="1">MTSAANILGGFGKTNGDPPSLSSASSASYGLNSNFNKMKIHSTVHRKSGWVNVKDEGIISFRWPKKYLVLGETSLDFFKSDDKAELWYRIPLLHVSSCVKNQMKENCFEIQHKGKSTFVCAKSDQEMNMWIDSVLAKCPKEGFSKPLKVTHEAKVSYDASNGTFKVKGIPPEWLALLKGSNITYEDFAADPSAVIDVLNTYSEMVSPELSHSDKFTKSAMSSIQKTLAGNANPSNPSPLSNEKSAPSNNLLNEITKPYSQKDSLSDRFLQPRRAPPVPNSDNETNSKNSSAANTSSGSNDSHKYIPARRAPQVPGQSSNTQQQIKQFTNNSSTKPSTKPPSQSPPIAGASSSISSIGSGKVKVSTSNLPSNQTSGPNSASSTSLHNVVNTSQTDIGLPVRQAPTTHQNQHHPSKSNGTIDHTLIPRRAAPAKPQYIGQHPQQQQQQQQQQQQQQQQQQQQQQQQQPQQTQNNGLINNQHIPLQQKTKQFHSNAYPKFPNQHQHQQQHQHLPQSQPYMQHQPSQPNMQRIPHPQQPYHKQQIPPQPTNPYQTPMTYQQKLPQQFKPTNTNNNNMMAQSSAHAKLMGVGATEMKPPRRPADKRISTMTEAQIMAKLRSVVINANPTPYFQIISKAGQGASGSVYLARSMRLNGQCIAIKQIDLTKQSRKELIVNEILVMKDSQHPNIVNFIEAYLNGPSDLWVIMEYMEGGSLTDVIDNNPKITEEQISCICHETTKGLQHLHKKNFIHRDIKSDNVLLDASGNVKITDFGFCAKLTTEKSKRATMVGTPYWMAPEVVRQKEYDEKVDVWSLGIMTIEMIEGEPPYLNEEPLKALYLIASNGTPTLKHPELLSNEIMDFLTVCLCVEVKFRATTDELLHHKFFKKACATSQLANLLTFSRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTM4</accession>
    <name>A0A1E3NTM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71026</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020594.1">
        <property type="nucleotide sequence ID" value="XM_019163805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTM4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTM4"/>
    <dbReference type="GeneID" id="30180492"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="71"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="273" mass="31901" checksum="2C11C5E617EEFD42" modified="2017-01-18" version="1">MSLEQLTAKLTENFGKEDYESCLKLLTPIKIQLIEHNLFVPSVQSSVNPNDLLITRSILEIGALVSINLVQMQEFSNYIVQLKPFYDLEHIKTESNQKNKLLSLYLLLLLTQGDLAMFHIELENFQNHKMSVDELEKDEFLSIPIKFEKWIIDGDFNKVYETLSSKHKFPCKEFNLFEDQLLNSIRLNIANDLEKVYKQLPIENLKLLLFLKEITETQEFIDNFDWKLSNGIVDFRKKEQTIGEQANIIDEINDEKAIIKNSFVYATEMESII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTR2</accession>
    <name>A0A1E3NTR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylserine decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.65</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_24586</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Phospholipid metabolism; phosphatidylethanolamine biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020489.1">
        <property type="nucleotide sequence ID" value="XM_019162357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTR2"/>
    <dbReference type="GeneID" id="30179044"/>
    <dbReference type="OrthoDB" id="4330at2759"/>
    <dbReference type="UniPathway" id="UPA00558"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010636">
        <property type="term" value="P:positive regulation of mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010954">
        <property type="term" value="P:positive regulation of protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016540">
        <property type="term" value="P:protein autoprocessing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03208">
        <property type="entry name" value="PS_decarb_PSD_B_type1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033661">
        <property type="entry name" value="PSD_type1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF6">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ49376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="372" mass="42059" checksum="B05BD23B58C6D890" modified="2017-01-18" version="1" fragment="single">WPLYLYSYLPLNTLSRLWGSFNSVTLPVPLRVPGYKLYSFLFGVNLDEMQDPDLTHYKNLGEFFYREIKPQSRPIDPTALVVSPSDGRVLKFGRVDPDGGIEQVKGMTYKLEALLGAAESQQTAHSMSLSYENEAHRELHQAHENDDVFLSHLRNNTNHDCTARADDLDQQSLVTFSQQGEKSNFHPSTSQVLNVSVALTPTTKETDLFYAVIYLAPGDYHRFHSPVTWVTTLRRHFVGELYSVAPYFQKAFNNLFILNERVALLGYWKYGFFSMTPVGATNVGSIKINFDKDLVTNTTMLNGEKLKKNTCYEAHYSNASSLLKGEPLVKGQEMGGFMLGSTVVLVFEAPKDFKFTLQEDQYVKVGQCIGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTZ8</accession>
    <name>A0A1E3NTZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_70723</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020271.1">
        <property type="nucleotide sequence ID" value="XM_019163723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTZ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTZ8"/>
    <dbReference type="GeneID" id="30180410"/>
    <dbReference type="OrthoDB" id="2264563at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF15">
        <property type="entry name" value="ACTIVATOR OF SKN7 PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="699"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="813"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="903"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1070"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1179"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="833"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="864"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="918"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="932"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="989"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1071"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1108"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1185"/>
            <end position="1194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1203" mass="132898" checksum="C07C283238CAAEEE" modified="2017-01-18" version="1">MEPDYGSIQRAQSSQELLSPFTTTSSSSSSVSHSSASSSKFSKIWLFDTQIFTAILIYVSYVRDQQAFNFLVRVFTQLILYSIPDTENLSDLRMLMSSEDLDLQITRITMVLVIVIPYTLMNFFFSMFLHMKWLYSSALLESSGLQISVDSIRNTINAITEKNLNSTTWINDGIFVDLIGESSMNCIQRTPINSSYLVFLDIAIISSRLTPKRESSAESLQTTASTVVGSGGAGNSSLLGMSEIKPSYHAPESVYNLETVYKNNTRFATQSVLPPNDYHSPYYIPFKNIDDPRPVDQLIYRFSIWKMIIKQVIFYFKEMSVFKSQTYQGNRAMLENLEILKRQNSGKLKVKANANKSLKKSLSSTSFGDMYNNATGGGNIGGGGGMNGSSTNLSHGANVNFNPSFSGGGGGAGGGNGASSIMSGSTAGASSISLDNLENEMNMDQQNMVNKLLQTTFLPAGDNSILSISSTFFNNHNYLKEKELLTYQHLTNRLIPRLENLKDQLNETIKQMNSIKNSSDYKTKNLKMEIAKTGGIMSDYITSIELLRTGHSKSSLGTTIELKNNRIDSKMDPYILKLRLDLQLKDQLYIEAHLKEMYYDLQYKAVQLEKILYTEIQNCISTYTDLIDSELNAVKDNMIMPFKDGFVKNDPTIDWDYFIKNDCKRNFLPINAKNTILKVKKVRKNSDIVYPYRNSVISSCIYSGYLDRKSKYLKNYSKFFYVLTINFLHEFRSNDRIKDLNPTQSFSLTDVTVSAVDDDPKKFVIRVYKKGDKETKTKFTFKADNIDSAIKWIDYLSDLTEFENTIERNISYDASDDENGYDDHNPSAGIPTSVEQNNFADNNSTVSSPASNSYEFNQRPTGALSSPESPSLVSHSRSISDELFPKSSQYQQVQNSLQDVDYFSKTHNNNTNNLGNVTFKSIKSQPNMRQTSSSSISLGNSNSKASSRTSSRASSRASSPARSRNNRPLLSPKIVSSNITPTSGNSTASGGGNGGGGGSGGGDYFSYAPRKVQASIIRNGNRLASTNNNGRLTPGTNNNSALTLNVAMAKNLSSSSVNDKLNASVPKINIHDSTPVNTEKPNINSILDGNYESPPTASSQPISNEPNEEPKNKGLERRRKTLSEKLDITGLVSANKIKNNGLLKLSNDAESLNSTAGLPQTPGIIPGSMMNEVFDTSNNIYSHASSPPETISASSPPPPPPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUP7</accession>
    <name>A0A1E3NUP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70855</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020416.1">
        <property type="nucleotide sequence ID" value="XM_019163755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUP7"/>
    <dbReference type="GeneID" id="30180442"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="258" mass="30356" checksum="6720B96F090BE147" modified="2017-01-18" version="1">MKSPTTGSIQSLYSGISPKTKSLLAFTLFQDKYQLLWFLSHINTLLGFFMYSAWVFNPEKHFLSVIPWYKLTNYSCILTYTIVIYKRYLCEETFEQNEAGGRILISYILKTENVQLIISAFLWTATNESFFKLVPFAIYSILNISCFLVFEAYPQSHFSVALAPFISYIKNPCLIFSAFVDIFIIGLLLNESYHNNSYYVFFLYIFVWGLKMDNSEASRIAVYKIMKVIDLGFNHKFVPYKITHYWVCFKQRIFRLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV37</accession>
    <name>A0A1E3NV37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PICMEDRAFT_71073</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020641.1">
        <property type="nucleotide sequence ID" value="XM_019163820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV37"/>
    <dbReference type="STRING" id="763406.A0A1E3NV37"/>
    <dbReference type="GeneID" id="30180507"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990332">
        <property type="term" value="C:Ire1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13982">
        <property type="entry name" value="STKc_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000077">
        <property type="entry name" value="Putative Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000572">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="745"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="22">
        <location>
            <begin position="1023"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="128"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="628"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="628"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="679"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ49528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1154" mass="131052" checksum="9E4CA76853077BED" modified="2017-01-18" version="1">MRVGQRQKRHVRVWWILFVTLVAFYIIPQRVRSEKTEGFSISDNYYIPENSNNNKELIVKPEYPVAETVYEQIDNFITQNGMARMDDFQITNLVLASDIEGNLNAVNRKTGELLWSLVGGQPLVSIRSDADTSDAGHTTPVASSSHPTDTSTREYRRYSNADMFESSHTKSSGYDSRRHTQNHKKQDVTWLVEPYGEGTVYHFMGDEGLEKLPISIKQFVLNSPFSIDDQFVYTGSRISGIVKINARTGAIVESYGLDGVGTCPSTDLDDAPGNLNTQYNYDDNDYGDGDDISYNFEKQTDYFGSHSHDSEDNEAGPFIKLGKTIYDLTIHSKNNTSWNITYVQWGPNNLHANLVTQNTNSADDTYIQPFHDSSLLALDVSSKSIKWVSNLPYVTVNIFDVFANSLVDNDYVVLPHPLDYGSSSMPKDKEESTYLDRTKEGSWYALSERHYPSLVRSAPIAKYITNERWRLPPIFQNDDLLEISISGVHDNSLRRHLKDDSQRNINKHLAVYQSATAMSIPEKNNGLTYSPGQSKKVTRSNRNHEIDEDIFDENVLRAPTLTFKSSSISKLVYRAVENVFVAMICLGIFYVMSKFGLLKAFINNSKAKDSSRPVKMVTIETDPTVDQKGKFEKKETTTLESSDLRRIVSKEDSDDTIRNTENNEKENNEDKDETEESARRKRKRGCRGGKKNRKKESIDNTGVSTAITSSSTDDDDSGAGIASVKTLEAMDESLSKITNISNSLSITEHILGFGSHGTIVFKGFFENRPVAVKRMLLDFYDVASHEINLLQESDDHSNVIRYFCSQEKNKFLYIALELCSSSLEDLVEKKKENLDVTVETSEILYQIANGVNHLHSLKIVHRDIKPQNILVALSKNNKNKNPVRFLISDFGLCKKLDADQSSFKGTTANAAGTSGWRAPELLLDTSPAYVDSSLANRVRLTRSVDVFSAGCVFFYVLTGGYHPFGDRYIREGNIIKGEYDLSKLNTLPDAYTIKDLISSMIHKNPMARPDISVVMKHPYFWSVEKKLEFLLKVSDRFEVERRDPPSDLLLKLESIASDVLGPKGWFDKFDAKFIDNLGKYRKYNTHKLMDLLRAIRNKYHHYQDLPKDLAMEIGSLPDGFYWYFSGKFPRMLLLVYRLMKTILHDEELLAQFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND65</accession>
    <name>A0A1E3ND65_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_18542</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015177.1">
        <property type="nucleotide sequence ID" value="XM_019162051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND65"/>
    <dbReference type="STRING" id="763406.A0A1E3ND65"/>
    <dbReference type="GeneID" id="30178738"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF36">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08340)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="64087" checksum="161B9639A1879CFD" modified="2017-01-18" version="1">MSSFKESIWQFFKIDNPSTFREDYIDPTIDRERKPEKVSKSDKYNILKAFNWYPSHYSKYERKFLLKFDFCVLLFLSASFYTKYLDNANVGSAYVSGMKKELNLKGNELNYFNTCYTVGYAVFQIPITLLITKPHFSRHLLLFCELAWGMMTLANAYVQNANQMYAIRFFIGVFEACSFPATYVIISSFLTEKEFFQRAGFYGAFAVAGNASAGALQTSAREHLDGIRGLSGWRWQFIIDAVITFGIFLYGFLLFPGIPSSCKKFGFFSEDEMIFARKRLENRVAFPKKFTWRTLKETLLTWQIYVGSALWVLHHQSWYSNGAKLYMKSRPDLYTTSMVTNWDSYMYCTGIPAAIIVAPLCKYYGKIIPVTLVMLTAYYACIILVIWDVPNAVLISAFFLQRPFRDGLAQVYYTWIATLCSDNVEKKAIVLSWVQALSYAVNAFAIPLQYNVADGPRFKKGYIINFAFIGSAHIVFLVCWFCDKYDWKYFPRFAGNRHLNNKDDISLTYDTSTDDSRTEIVDIESVGGTGVSKNQNGVEELFVRDREVDHTDSSSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND95</accession>
    <name>A0A1E3ND95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015205.1">
        <property type="nucleotide sequence ID" value="XM_019164920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND95"/>
    <dbReference type="GeneID" id="30181607"/>
    <dbReference type="OrthoDB" id="5582146at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133310" evidence="1">
        <location>
            <begin position="23"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="244" mass="27761" checksum="BA126F352F486B49" modified="2017-01-18" version="1">MVSLQHVALCALVLERPVLVTSTDPHTTVKRVQKILQGWPVNSQVTPITTANGASVFSLLDMSLENQRTLYRELRKQKYPLVLVSSPESELDTKLETLLLFNYKDELNDLPQPSELFRFTSLEAIRKSVRQVTITTEMKTYIYDLIVEVRYSRFIKGGIPTYIIADLTDFIRFKAFILEMTFVTPAIVKTCFKIVLPLRLHLIDPEDDPSLLYGSNPTLVQQLIRAIDANDVIDIAISNVRPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDE0</accession>
    <name>A0A1E3NDE0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_24607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015250.1">
        <property type="nucleotide sequence ID" value="XM_019162359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDE0"/>
    <dbReference type="GeneID" id="30179046"/>
    <dbReference type="OrthoDB" id="2103474at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF1">
        <property type="entry name" value="YALI0C06193P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="160" mass="18227" checksum="F56CF3C88CDE8476" modified="2017-01-18" version="1" fragment="single">DRVNEYAEKHVKTKDPYREDVGGGKTRKLKLRDSATKEEQKMWKWVQRRAWVDDKCFMGCYPVDCGVGLGPLVVALPCIGPLLMYAVHARLTTRAGMFFNLDAKTVAKLNANILFDLFLTFPPVIGSFFSWMNGCSSRNAAIIHSEVTKMLLRREQAQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE61</accession>
    <name>A0A1E3NE61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_27849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015540.1">
        <property type="nucleotide sequence ID" value="XM_019162518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE61"/>
    <dbReference type="STRING" id="763406.A0A1E3NE61"/>
    <dbReference type="GeneID" id="30179205"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="66" mass="7507" checksum="D3218436323A95DB" modified="2017-01-18" version="1" fragment="single">IDMGTHYATLGVPQNASDAEIKKAYRKLALKLHPDKNPSKEAEDEFKQVNDAYTILSDPTQKRKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEF5</accession>
    <name>A0A1E3NEF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_74741</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015623.1">
        <property type="nucleotide sequence ID" value="XM_019164856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEF5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEF5"/>
    <dbReference type="GeneID" id="30181543"/>
    <dbReference type="OrthoDB" id="1747252at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="276" mass="29953" checksum="A9AD7F5234B7168C" modified="2017-01-18" version="1">MLALRRIPAVAADSRSLARSFSTVQPLLAPKNKKELEAKKALEKRQERRDAVKKLEAKKPASSSALFMSVKDALKYLRATEVGRSLNEASITIQTPILKDRGVAPLQGAVRLPKPLKETKILCLTNDEAKIAEALQNGAAKAGDAKLIDEIANGTLSVEGFDKVLATPDMEPLLRKVSKILGPKGLMPNVKRGTISEDLNNLILSTLGTQPFRERNNYVSLTIGKCGFTDSEILKNILATSEAFKESVQNTKSKKPILMGQTILSSTHGPGIVINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEJ7</accession>
    <name>A0A1E3NEJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_18405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015629.1">
        <property type="nucleotide sequence ID" value="XM_019161987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEJ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NEJ7"/>
    <dbReference type="GeneID" id="30178674"/>
    <dbReference type="OrthoDB" id="3997667at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133317" description="CFEM domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="7">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="25"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="197" mass="18855" checksum="DBFF19374FB09BE8" modified="2017-01-18" version="1">MLSYISLAVLVASFVAPSAATPPACLLACAASVVKSSPTCNQLNDVTCVCNSEAEALQACLSTACPEGVASKASSAFKSVCTAATAKSSATSSAAWTAVPTAEANFAAASGIVRSSGSAVKSSAVKSSAVKSSAVESSAVESSAVKSSAKSTTLAKSTSAAASSSAAHSASSASNAAGSLEFNGFLGVAAAVAINLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEP3</accession>
    <name>A0A1E3NEP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PQ-loop-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_24166</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the laat-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015705.1">
        <property type="nucleotide sequence ID" value="XM_019162339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEP3"/>
    <dbReference type="GeneID" id="30179026"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000009">
        <property type="entry name" value="PQ loop repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF34">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="30896" checksum="FDB16F105D9E3683" modified="2017-01-18" version="1" fragment="single">LSGLSGYLSISFWLFAQIPQVIKNHTDKSVDGFSLGFLLCWFAGDFLNLVSCLLNDAMAFQILLSGYYCAIDLVLAFQYYYYKRMYHNPESRWYHPPRRRGKHARSPRTSLRDNLETYGSIDTEMMIDQRMLQQQQPQGQRTGVFKKQNAGISKLVTSALISGFTKVQGLPIVQQYIDTELLGKILAWACTALYLVSRIPQIYTNYKLQSTRGISLKLICFALCGNFFYSASLLLSENSTVGGDVSREFWKAELSYLIGAVGTVVFDCSVLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEQ5</accession>
    <name>A0A1E3NEQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Carnitine O-acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="16">2.3.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_74305</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-carnitine + acetyl-CoA = O-acetyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:21136"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57589"/>
            <dbReference type="EC" id="2.3.1.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.7" evidence="16"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015689.1">
        <property type="nucleotide sequence ID" value="XM_019164733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEQ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEQ5"/>
    <dbReference type="GeneID" id="30181420"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000007">
        <property type="entry name" value="Carnitine O-acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="20">
        <location>
            <begin position="22"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ44576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="612" mass="70214" checksum="FDA8CB6AD6166289" modified="2017-01-18" version="1">MRRTTTTPQGDLYKHQNDLPPLPVPKLAHTLDLYKESIKPLYPYGEKDEEFIKYAKIIDEFATGTQGSGLQSKLERFAADKRNWLAAFWDNYAYLDYRDPVSPFVSYFFSHRDANTAVGKDQLLKSASLTRKVLEFMETVEHETLEPELIKGAPFCMESFKWMFSNCRVPGESRDYNLVYDGSKNRFMIVISNGHIYKLQTHDDATDSILPFNALYYGLAEVLKDSEARGPNETPVGVLTSSNRDEWFSNYQKLNEISIVNKVNFEDIFKSSFVLCLDDCLPNTIEAKSRNCWHGNGLNRWFDKPLQFFVARNGSSGFLGEHSKMDGTPTLRMNDWLVGEMYKLDIDGNVAKPTTVPAIRQINFEINNEISEAIKTETETFNETVNSLDIKTWQYFGLGKDDIKAFNCSPDSFVQMLIQLAYYKYTGTLRPTYESASTRKYFRGRTETNRSVSSEALQFVKDWENPSVPVQEKLKSFRAALKSHGNYIKLASSGLGVDRHLFGLKQMIGESDEDSEVIKQFFDNPIFSYSQYWYLSTSQLSSENFNGYGWSPVVPEGLGLAYMINRKWLHINITNYKENPFGLKVDEMAYFLTLSVQELAEALRKETVKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NES1</accession>
    <name>A0A1E3NES1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="11">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_18011</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="11"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015709.1">
        <property type="nucleotide sequence ID" value="XM_019161848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NES1"/>
    <dbReference type="STRING" id="763406.A0A1E3NES1"/>
    <dbReference type="GeneID" id="30178535"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="13">
        <location>
            <begin position="24"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="12">
        <location>
            <begin position="370"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="202"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="342"/>
            <end position="345"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="10">
        <location>
            <begin position="61"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="489" mass="52948" checksum="A737971C403B71D4" modified="2017-01-18" version="1">MLKQTHIRLFSFTTRRPSCTKKHEVVVIGGGPGGYFAAIRAAQLGLDTACIEKRGSLGGTCLNVGCIPSKALLNNSHLYHQVKTESAKRGIDIQGKVKPNLVNLMKAKDAIVKQLTGGVEILFRKYGVTYYKGNGSFVDEHNVKVDPIDGGEETVIEAKNIIIATGSEPSPFPGVDIDQERIVDSTGVLSLTETPKRLAIIGGGIIGLEMGSVWSRLGSQVTIVEFQAAIGGAGMDAEVATATQKFLKRQGLNFQLGAQVRKATRNGDEVDIEFEDVETGKVQNIQSDVLLVAVGRRPYSLGLNAEAIGLEFDRKKRVVIDPEYRTKYSHIRCIGDVTFGAMLAHKAEDEGIAAAEYIKLGTGHVNYENIPAVMYSHPEVAWTGKNEQELKAAGVKYKTGKFPFVANSRAKTNMDTEGFVKFIADAETQKVLGVHIIGPNAGEMIAEAGLAIEYGASCEDIYRVCHAHPTLSEAFKEAAMSVFDKPINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF56</accession>
    <name>A0A1E3NF56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Class I unconventional myosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_18465</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015321.1">
        <property type="nucleotide sequence ID" value="XM_019162009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF56"/>
    <dbReference type="STRING" id="763406.A0A1E3NF56"/>
    <dbReference type="GeneID" id="30178696"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071852">
        <property type="term" value="P:fungal-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11858">
        <property type="entry name" value="SH3_Myosin-I_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000015">
        <property type="entry name" value="Myosin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035535">
        <property type="entry name" value="Fungal_myosin-I_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF837">
        <property type="entry name" value="MYOSIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="9 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="17">
        <location>
            <begin position="47"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="18">
        <location>
            <begin position="791"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1108"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="967"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1025"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1171"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1214"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="968"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1083"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1093"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1237"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="140"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ44208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1246" mass="138637" checksum="B481A6D2E2B5F064" modified="2017-01-18" version="1">MMGFNKRTRQKTAAPVRAGGSTAGGSSSGANGGIKKATYDHAKKKEVGVSDLTLLSTITDEAINDNLKKRFENGIIYTYIGHVLISVNPFRDLGIYTLDVLMSYKNKNRLEVPPHVYAIAENMYHNMIAYSDNQCVIISGESGAGKTEAAKRIMQYVAEVSGSLTNSTSIQRIKDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIQFNKSFEPVSANITNYLLEKQRIVSQISNERNFHIFYQLTKAADDNYRQTYGIQQPEQYIYTASAGCTTVDSINDTTDFADTLKAMETIGLSKDEMDNVFRLLSGILWIGNISFVEDEEGNSQIRDTSVTNFVAYLLQVDAELLCKSLVERTMETSHGMKRGSIYHVPLNPTQATAVKDALAMGIYNNLFDWIVDRVNQSFSKNNQAIFKSIGILDIYGFEIFEHNSFEQLCINYVNEKLQQIFIQLTLKSEQDEYVKEQIQWTPIKFFNNQIVCDLIESKRPPGIFAALNDACATAHADSSAADQSFAQRLNMLSSNAHFDLRGSKFVIKHYAGDVTYDINSMTDKNKDQMLKDLLEMLQTSQDPFLKALFPNRVDESNKRRPPTSGDKIKTSANLLVDTLSQATPSYIRTIKPNQHRSPSEYDTKAVLHQVKYLGLKENVRIRRAGFAYRQTFEKFVERFYLLSIKTSYAGEYIWTGDARTATEIILKDSNFDPSEWQLGVSKVFIKKPETLFALEDLRENYWANKAKVIQRACRKWLRRREEAARIVQRLWRARSKGISQENQYTEMRDYGNKIVYGKKQRRRLSLLGSRGFYGDYLGCNDKTGGFGKFIMNSIGLREKVQFSAKGEQLHSKFGRSSQRLPKIFIITRDKFLVVGQTIVNNKAKYTIEFNKLITQIPEVSVSNLADDWLAVNLHSKTEADLFVNCIFKTEMLARFKKGNPNIKISISSTVVYCKKPGKKHTVRFKLAASSAKTGDAYKSSTVSVSQGTEPTARNRETPRIKTAFTGDKIAAYKRSAQSRGLKNSMTAAASAAYHPATPNANSHSNGLSKPAPPVSRRREAATSSVPAPPPAANKPKLTVKSKQAPPVSRNRIPQTVPAATNPTPTPPPPPPPPPAAAPKWPTYRAEYDYPGTNGSMPLAKGDIVYILQSEPSGWSLAKTLDETTEGWVPTAYIAECAPPANGGGHSAAPPAPAAPGFSNSTPAQAATGNGNIAGGLAAALMEKKQEETTMASDLAAAIRQHARRESDDEDEDDDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF59</accession>
    <name>A0A1E3NF59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear condensin complex subunit 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_26210</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CND3 (condensin subunit 3) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015890.1">
        <property type="nucleotide sequence ID" value="XM_019162438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF59"/>
    <dbReference type="STRING" id="763406.A0A1E3NF59"/>
    <dbReference type="GeneID" id="30179125"/>
    <dbReference type="OrthoDB" id="27187at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903342">
        <property type="term" value="P:negative regulation of meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027165">
        <property type="entry name" value="CND3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025977">
        <property type="entry name" value="Cnd3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418:SF5">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12719">
        <property type="entry name" value="Cnd3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear condensin complex subunit 3 C-terminal" evidence="10">
        <location>
            <begin position="577"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="512"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="915"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="512"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="949" mass="109686" checksum="CD23DE39575E00E7" modified="2017-01-18" version="1" fragment="single">RQYLNQILNISTLEDLQLIMRKAFQEAQTTVAVHKRQLAIMRAVILRANTLGLTSDFNSFFCHLISKVLPMKKNVKAADRIVKFISSFFSLINPMRVEEEEDLESDIENSQEMEELYSLFVEKVLKFLLGGLDALNTTVRFRICHLLSHIIGNCGGIDEDLYETLSDELTTRIYDKDASVRIKAVTALACFQNDDRGSLSLAGKKLRFTMQNDLNSEVRRACLRNIEKNRFTEPFIVERARDVDTTNRRLVYSKILPGFQKITDINSANRNRLLEWGLRDRDATVRKAATNWLTETWMRDTNNDLAEFLERLNVTESDIAETAVRALLDKKPDIVNNLKLESDFFDNLTPSLALMIRVVFEYCHDNRKSDIIENLFMEAADFASLFVRYFRLRTENLLKISENREEIEKNPEFAIELGIIDPDEYNYIIMQLLKIAVDYDYSDEFGRSKMYSILRSTLSNNSVTEPILPLLMECLRKLAINERDFCQMTVEIINDLKDSEYERIMNLKKQEEDNRKAEEEALTKKSDDDENDEDSDYEEYHSAMNDMSRQSIIEANKSRQEEIDQVSVLSPDIITDCLTITKCMLQLVFSPLRENMLLISLLDNFIVPCVNQRSEVTVRELALTCHGLCGLLDKEVAVSTMVVAGIFVTRSDHESFVVTGLKVIGDLLTIHGISILQSENQRSIDSMAVAKVFYRTLKDESKPEAQSIVAVTLFKLFLCGVINDDELFETTLLTYFNPKVNKNPALKQCLAFCIPTYSFSHENHQELIASIVYDTIERLYKDWDDIVSLNHSLEIKQPVTANFIIESLLYWTDPYNRALASEEEMESSSLHLDVGIQLMRLLKKYDYQNKTHKAQYKPIIRSLTKLTFTSKANIEKLRKFRECFDDDELCQGDLDEVLNSDLICKNSFLKCHTYIQECLAEAEDRERERLEKEAELVSQRSVKQEETQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF98</accession>
    <name>A0A1E3NF98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YbgI/family dinuclear metal center protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_36732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015879.1">
        <property type="nucleotide sequence ID" value="XM_019163032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF98"/>
    <dbReference type="STRING" id="763406.A0A1E3NF98"/>
    <dbReference type="GeneID" id="30179719"/>
    <dbReference type="OrthoDB" id="3345469at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="31304" checksum="BCD6B47099EE0F35" modified="2017-01-18" version="1">MSVRALESRVLQTVTRAMDKLYPLKNAENSWDNTGLLVDSSVPDAGDAAAAAAPRILLTVDLTEAVADEAIRQRCNVIIAYHPFLFRKFNRITPADNTQHRSLVKLIRNNVSVYSPHTAVDAAVGGVNDWLADGVCAGSVETARRVIVPDKTAVPGVGMGRIVQLDRPIPLSDAVARIKTALHLRHVQVAARAGVDAVSVRTIAICAGSGAGVFQNLDPADHAAVDLFYTGELSHHELLALSEHDKSVVLCGHCNTERPFLHTLRTRLAAELAPVAGAHVVVSLCDASPFVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFK6</accession>
    <name>A0A1E3NFK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_25453</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016023.1">
        <property type="nucleotide sequence ID" value="XM_019162393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFK6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFK6"/>
    <dbReference type="GeneID" id="30179080"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052239">
        <property type="entry name" value="Ser/Thr-specific_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="316" mass="34580" checksum="6B39851D60BE9911" modified="2017-01-18" version="1" fragment="single">TIRINSASFQLLSVLGEGSYSVVYLVQCSSGAARGRYALKKIRTISTLSTQRALKEVAYYGQFRSPYLIRALDSSSIQEPDGSRSIYILLPYYAGGSLQDLVNSTIFDPGLRLAEHDVLRLFVAVCRGVLTLHRYHAPHAPHAPDDDGAAAAAGTTQLAEAVPYAHYDLKPDNIFVARDGTPVIGDLGSCSPARIHITSRAQAVSFQELVAENSTPEFRAPELFDVRPNDSFDERVDVWSLGCLLYTMLFGVNPFVREVRRSGANLKLAVQAGRWSFPDQDPDDPDTPAYSDLTTALVQQALTVDKEARPSTDTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFS6</accession>
    <name>A0A1E3NFS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Multiple RNA-binding domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_35481</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM MRD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016103.1">
        <property type="nucleotide sequence ID" value="XM_019162948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFS6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFS6"/>
    <dbReference type="GeneID" id="30179635"/>
    <dbReference type="OrthoDB" id="439639at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12565">
        <property type="entry name" value="RRM1_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12568">
        <property type="entry name" value="RRM3_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000247">
        <property type="entry name" value="Multiple RNA-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000459">
        <property type="entry name" value="Multiple RNA-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034482">
        <property type="entry name" value="Mrd1_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="321"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="504"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="629"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="730"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="149"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="254"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="582"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="817"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="584"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="614"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="828"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="853" mass="96546" checksum="B7E64C205ABC9C9C" modified="2017-01-18" version="1">MSRVIVKGLPTYYTEEKLRDHFSKKGYVTDVKLKRKNNGESRRFAFIGYKDIADAENAVKFFNGSFIDTSKIIVELAKSFSDPNVPVSWKERRKERDELLQRTEERLQQMEEAANRRKAKKQKKVNHLNDKIENNSNLKEFVEAMKPAAQSRSWANDEVVNTEGAPTNEKLEEALGLTKNGSDDSDDEYDDNLVSGKTKTKDDNVNENSEEKMISLNDMVSDEKDDETNEPNGKDENMDDLDWFKSKRKRIIDTQEGDEAKNVEPLNVSQSQTPSGIENGKTEVEAVASGEDEEEEDEEKEETVRELSLEERNIDRILVTGRLFLRNILYSSTEDDFRKLFEPFGALKEVHVAIDTRTGKSKGFAYIQFETAKDAVKAYIELDKQIYQGRLLHILPGEVKKEHKLDEFELKNMPLKKQKLLKRKFEASKQEFSWNSLYMNTDAVLDSVASELGISKNELIDPTNSSSAVKQALAEAHVIGDVRSYFEKKNVDLTKFSEKERDDKVILVKNFQFGTTKEEIGELFAAYGELNRILMPPAGTIAIVEFRDAPNARAAFSKLSFRRLGKSILYLEKGPQGLFTKQADQSDKFQVEEKTTETKPNASDVLESTKNEDVENNEDSGDEDTGPTVSIFVKNLNFSTTSATLSNIFKAYPGFLLAVVKTKPDPKNPGKTQSMGFGFAEFKTKENANAALRTLDGFTLDGHKLQLKISNRVGNTNEISNSKKKKAKSGKIVVKNLPFEATRKDVFELFSSFGSLKSVRVPKKFDKSARGFAFVEFATAKEASNAMDQLQGVHLLGRRLVLDFAEQESDDVEAEIEKMTKKTKSQAKSRELADLREGNSGKRKLELEEEDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFV2</accession>
    <name>A0A1E3NFV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_59859</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016138.1">
        <property type="nucleotide sequence ID" value="XM_019163393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFV2"/>
    <dbReference type="GeneID" id="30180080"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF25">
        <property type="entry name" value="FERRIC_CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133356" description="ferric-chelate reductase (NADPH)" evidence="16">
        <location>
            <begin position="17"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="17">
        <location>
            <begin position="395"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ45025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="652" mass="75347" checksum="BDEF9B35F523565D" modified="2017-01-18" version="1">MKVLYLLVSLVVIANALVISDSHVASSCIYFFRKKSWDCNSASAGHMNSNIWNCQCLNIEWLGSITNCINDYSNSTKERDHAYEHIRIRCNGKAKTNYTLDEMKEYQLNATKYLESADLFPKGTNVTTPIEVDAKSFAWWYKTFRDYNYFISMCQRLGWGCVGFWIGIVGLSGIYNYVGYKFIPYSTRQIFNRHLTYRYDAYCFGLNRLELLICFLFFVQVLLMCCINYSLELNSYLYTRWFLLLDLISFRTDIIAFSLMPVLYLTGIRNNPFQYFGCMSHATMIKFHKFIAIIFFILAVVHSVIWTHYARSKDGGGYAVWAADAYFYWGIVGTIVIGIMIGFSIELVRNLLYEVFLFFHLSFSVLFVAAMWKHCETLGWMGWVYSLVAILCFDWVARFTRIASNGFINSAQIEIYGPELVKLEFEKPVVMKFYPGSYVYLHILSPVWSCWQSHPFSLIRSTEADNKMIVYLKCKRGLTRRLSRLNEQKNINVLIDGPYGVLPVKTSDDEGFDEVIGIGGGLGISSVLAFFNERARQSKLVSNYKLNWLINDLKQIELISNDLEYLKKSGMKVTIYYTRYDDSSESAETEKDTDSSHSFVQTVQYGKPSIETLLPQSSLKRRVYICASESLIQSARHNLSPLDELIVENHTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFW9</accession>
    <name>A0A1E3NFW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73775</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016089.1">
        <property type="nucleotide sequence ID" value="XM_019164582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFW9"/>
    <dbReference type="STRING" id="763406.A0A1E3NFW9"/>
    <dbReference type="GeneID" id="30181269"/>
    <dbReference type="OrthoDB" id="1915122at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031334">
        <property type="term" value="P:positive regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040153">
        <property type="entry name" value="Rcf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018:SF3">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="6">
        <location>
            <begin position="87"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="197" mass="22373" checksum="A54E432C914B6D80" modified="2017-01-18" version="1">MKLITDEEKRAHNRSVLHGGLKGLALGTCLSIGIYAMAPRHYPRIFSLPWSIRTAIFVIPPAFAASVNGELCSNEFDYDMYSSEASQRRLLAEHRRWEGLTTSEKIVESVSSRKYLIITGLWAASMWGSWHYVNKDPLLTKTQKFVTARMYAQFLTVGLLLGSIGLSMWEENINKNKKKIEFNPDDEALEEVLSQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFX3</accession>
    <name>A0A1E3NFX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_73833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016150.1">
        <property type="nucleotide sequence ID" value="XM_019164602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFX3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFX3"/>
    <dbReference type="GeneID" id="30181289"/>
    <dbReference type="OrthoDB" id="3980246at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053720">
        <property type="entry name" value="Psm_Assembly_Chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018854">
        <property type="entry name" value="Psome_chaperone_3/4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10448">
        <property type="entry name" value="POC3_POC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="164" mass="18712" checksum="7337777D2B5D69B9" modified="2017-01-18" version="1">MSIGNFRDNVKELDLIIKRDGREHMLTCTMLEFSDKLIIHLSMNGETDISYDIQIPEKNDLKKPLRRYDFDEEKEQEELENTIIPIVLIGSGNNMKMQVLSSQIGHTISQLSNKNIILNISGKLFGRAYLDEYRPGDSVLVQQCLHLATETYQSQRPAADVSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFX4</accession>
    <name>A0A1E3NFX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_164553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016153.1">
        <property type="nucleotide sequence ID" value="XM_019161211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFX4"/>
    <dbReference type="GeneID" id="30177898"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="216" mass="24121" checksum="0976F7C59F0915EF" modified="2017-01-18" version="1">MEQRRRGTRHDGMDVDHPQTVKKFVCCLCFAAFYCVLLRFTAFCCAYCVASRLVCMSVFSPGACCGGAQCQWCSGVGAVPLLCVSGRCPRPPPFCFLRLPLCAVHVCVLPCCCCAVLLQPQLRVARLGARFSSRVGRKAGQYCRRQLTPRGGCHVAKGHVTCNGSIDLSALPWKVCHVTWISRDIVWVDRLVFLHCPEKGVEFFFSLFFLFFSFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFZ8</accession>
    <name>A0A1E3NFZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_25290</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015386.1">
        <property type="nucleotide sequence ID" value="XM_019162387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFZ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFZ8"/>
    <dbReference type="GeneID" id="30179074"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0697">Rotamase</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="363" mass="41354" checksum="43A2A4F293D92BBD" modified="2017-01-18" version="1" fragment="single">WKLPQKRIFSSSDIPSFQSSKAIANINGIVSLICTKISLVNVPLGALNDELVNFNSKGFHHSVTTSEKVMQKHRDTTSVETPPKFADEAESRSYSTVAKAILGILQKFKDLKDQTPPFPGPRRYGNFACRDWHDKLTSNIDSILDDAFFDLLHEAEKSENYLGLKNEIRYYLLGSFGSRERLDYGTGHELSFVSFLGCLVMIDLINREEMKGSEWLLILAGYYDLVKSLILSYTLEPAGSHGVWGLDDHFHLIYVFGACQFTSNASINVMNYRMGLTPSSILNPDTLRRERDKNLYYNAISFIRMVKVGPFSEHSPILYEISASKNWEKIARGMLRMYYGEVLSKFPVVQHFYFGGVFFPWVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGF8</accession>
    <name>A0A1E3NGF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WDR72-like alpha-solenoid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016348.1">
        <property type="nucleotide sequence ID" value="XM_019164646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGF8"/>
    <dbReference type="STRING" id="763406.A0A1E3NGF8"/>
    <dbReference type="GeneID" id="30181333"/>
    <dbReference type="OrthoDB" id="4008582at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057848">
        <property type="entry name" value="WDR72_alpha-sol"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23123">
        <property type="entry name" value="WDR72_alpha-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WDR72-like alpha-solenoid" evidence="1">
        <location>
            <begin position="134"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="26423" checksum="1FF0EBA99029A3C7" modified="2017-01-18" version="1">MNQDTESLYVFLHSRIEREVMQLKQKHGAAGFGEDATDDELLLTYNQLLRYARDIDSIRVKVYESKQLNAFNKKLHVTDRDIQNLLLDAEMRIDAMYRKAQAQKELERKHVANAAIGGDISDDVETIGRYQHDEFEGRESIESLKSRLLSTRHDQLDQVESTELQNNYHDSIQQELIDSLPSMVSSLKDQALQFQEMIKQDAVILKEATQNFETSHGRFDNGDWGSGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGJ8</accession>
    <name>A0A1E3NGJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_12976</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016357.1">
        <property type="nucleotide sequence ID" value="XM_019159993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGJ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NGJ8"/>
    <dbReference type="GeneID" id="30176680"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021755">
        <property type="entry name" value="TF_Aft1_HRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020956">
        <property type="entry name" value="TF_Aft1_OSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11786">
        <property type="entry name" value="Aft1_HRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11785">
        <property type="entry name" value="Aft1_OSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="728"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="109"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="379"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="600"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="659"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="746"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="393"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="432"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="607"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="617"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="667"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="683"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="872" mass="95827" checksum="8942748664960506" modified="2017-01-18" version="1">MSLPSSTVTTNMVSISNTNSRSNPDLVSTFSNTKTLDLEPNPFEQSFAQNDDALLISGKVGPGKLNGSKDGISENLAGAADGSKPLIKPQSDTQLNNLRKYDEDAVLQKQAQHLKKQHQHQHQHQHQHQQQPPPQLQQQQPQQKQQLDSQNQQPQQEILPYSQSNTSLSRSSVANTNTPNSQHSQNIKQNEAPLDFNVKHIQQNLPKQPSNVSFNLSHRLDHSSMSPPVLTPGGTKRMASSVLPPLPGFLSPGGGSFIATPGIWNSLFPLTPGVQQQQQLLQQQNTPLNVASLGALSPNTLAMTQQQLQQQQQIQNKNFQNLQKQIQQIQQIQQTQHQGKGPSKQQLQQLQQLQVQQLQQMQQPNSHNLMDVTAAQLSDSANAEQQDKLTLDQRQQQQQPQQPQQAQALQQTQQPQQAQQHPQPPQPPQPPQLLQQQQQQQQQQPMQMYNFIMNAKRSGLTPNESSIRTGLTPGVNMFQNSSLYSYFPMAPNSQMPMNGSSLSVGSNNTNNTSITNTTNNTNNTTNTNNNNASNIIGVGSDIRKPLSTGLTGFNIPTLGNLDVKTLSNLDPGQTHQDMNNANIKGGISVDGQAGIVDGTQFNRSHKSSTSTTHTTTNNIRPTSDQASVSPASKDTIIPLANSSLNVGVMNESSYINEIPMPRAEKGTTATSNTRKRLSSSSEVNHDTKRAKSVKMEESSPNLTGSPESVSSVKAKSAKSKKKAPATEEEKRQNFLERNRVAASKCRQRKKEMIENMKYDLEEYKAENKLLRDQVGTLREHALTLRTILYAHRDCSLLIEQVGGIQSLNTILNATNYVAQIPEAHQSPENINADEVPQLLNGSGTNVQAAVAAAVAHTTNKAIERQGRLSTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGP1</accession>
    <name>A0A1E3NGP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycosyltransferase family 15 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_60615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015626.1">
        <property type="nucleotide sequence ID" value="XM_019163450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGP1"/>
    <dbReference type="GeneID" id="30180137"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="420"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="55173" checksum="F594ED30C6947835" modified="2017-01-18" version="1">MTDEQIREQLKNIQEEQLEREKEPHTENLLDDQDNVLGTKIANDHKRRQDLPPDSQPWARYEIDRVIPPKYKGNDATISRENATLFTLCRNSELYEILDSIQQLETRFNGNFHYDWVFLNEEPFSPEFIELTSNMVSGRARYGLIPRKHWSYPPFIDQERAKEIRESRKWSAITYGSSESYRHMCRFNSLFFYKHPIMEEYDYYWRVEPHVNFACDIIDDPFKFLKENKKKYGFTISMRELPNTIESLWATSKLYFKKLATNVFDAEQTHNLLNFISDDGGESYNYCHYWTNFEIADLSIYRNDIYEGYVQHLDQAGGFFYERWGDAPIHSIIFSLILDMDEIFLFQNISYEHTVAKTCPMNDAFRKEAKCICNPVEDWTIRSESCCNLKFLEVGSCEKNTDLDKYLNALREKQAADEELKAQQKKLRMETARRQSEARRKKTEERRKNRLQAQKEKKKVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGY2</accession>
    <name>A0A1E3NGY2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016504.1">
        <property type="nucleotide sequence ID" value="XM_019164690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGY2"/>
    <dbReference type="GeneID" id="30181377"/>
    <dbReference type="OrthoDB" id="274752at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="249" mass="28666" checksum="3D014C64180B3C3B" modified="2017-01-18" version="1">MARLNFLGFVISQGKMEKTIKVRILQKKFDNKVQKHFLQKKDYLVHDEGNICREGDLVRIEQTRPLSARKFFAVAEIKKNKGQQFATYQKEAKANVSQEESEKSQILQQKRALFGQTATESLYKDLSDIKEFKSKDNLTEEEIAKMNQIKEKYNVSLSTSEPGASKTREIFATTMDHLADKIEGLSSDLKVTTLLNEILNDESKKHIVDQICQKIEIKPETQKNIKKNLIRKYLRNTPASELEKLGLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHJ3</accession>
    <name>A0A1E3NHJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="15">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_16902</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="15"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016710.1">
        <property type="nucleotide sequence ID" value="XM_019161390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHJ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHJ3"/>
    <dbReference type="GeneID" id="30178077"/>
    <dbReference type="OrthoDB" id="445564at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000036">
        <property type="entry name" value="Serine/threonine protein phosphatase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="16">
        <location>
            <begin position="344"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="548" mass="62184" checksum="F0BBB3C0694B2365" modified="2017-01-18" version="1">MSTSSMSKEELYAHFKGLGNDAFKSQNFPLAIEHYTSAIKELESEDGHEDLAKKDDVNIMSENLEDVRTHSHKHAHALAILYSNRAQAQLKLENYGLAINDCTISLDHDSLFVKSLYRRAVANYAIRDLQTALKDCKQARAAAPNDNKIKVLTNEISQELKRLRFEQAIDVVEKSVFTSLNWDDMGTPDADSKDVKQSLEVEVTDEKKGDIVIKAGLTQKYINNMIEVFRDGGKLNKHDAFAIVNAVNTIFKQEKALVDIGFKKKTNPNSDYLHLENVEKLTVCGDTHGQYYDVLNIFKTFGEVGEKHAYLFNGDFVDRGSWGCEVAFLLYALKVLYPTRIFINRGNHETDDMNSVYGFKDECKFKYNERLFKCFSESFGSLPYCTLINDDYLVMHGGLFSDDSITLNDLRKINRLHHTHGQPPKTGVEMELLWTDPQVEEGRSLSKRGIGLQFGPDITKKFVEKNGLKGIIRSHEVRQEGYEWEHNGLLCTVFSAPNYCDAQGNFGAVIEMTCPEGDGEMKLESLKFEAVEHPNIPPMKYTKNQFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHK0</accession>
    <name>A0A1E3NHK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16882</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the small ribosomal subunit (SSU). Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 32 different proteins and 1 molecule of RNA (18S). The 60S subunit contains 45 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016689.1">
        <property type="nucleotide sequence ID" value="XM_019161379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHK0"/>
    <dbReference type="STRING" id="763406.A0A1E3NHK0"/>
    <dbReference type="GeneID" id="30178066"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14731" checksum="DD68623F121BBA6C" modified="2017-01-18" version="1">MTRSSVLADALNAINNAEKSGKRQVLIRPSSKVIIKFLTVMQKHGYIGEFEYIDDHRSGKIVIQLNGRLNKCGVIQPRFNVKINDVDRWTDNLLPARQFGYVIMTTSAGIMDHEEAKRKHVSGKILGFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHR2</accession>
    <name>A0A1E3NHR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16980</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016798.1">
        <property type="nucleotide sequence ID" value="XM_019161415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHR2"/>
    <dbReference type="GeneID" id="30178102"/>
    <dbReference type="OrthoDB" id="40021at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="2"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="170"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="346" mass="37477" checksum="BB74D444011D5C3F" modified="2017-01-18" version="1">MTLAERMVAVEGCRWCTTAVANAPYVTDPLVMDEYGCKYVVHGDDITTDKDGKDCYAEVKAKGRFIVVKRTPNISTTDLVGRMLVHTTEHHLARVDGQQWAQWQRVSAADAAQAGGKTLLTPENVERYRRYATAADARSAGAAVWVYTEAGVHELVKGQASAGTKPVYYVDGGFDLFNPGHITFLKRVRELADADGSLVVVGVADDAAVNSARGGSYPVMNLLERSLCVLQSKYIDGLVLGAPRAVDTPFLTLFKRGTGLSVHRVVHGPTTPLQADDYDDARATGLLLEVTTGLDLYASMTVESIVERVVANRAAFEERQRRKGVKAETEKSLESQEEAVRAGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHV3</accession>
    <name>A0A1E3NHV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Guanine nucleotide-binding protein alpha-2 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17000</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016822.1">
        <property type="nucleotide sequence ID" value="XM_019161423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHV3"/>
    <dbReference type="GeneID" id="30178110"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010255">
        <property type="term" value="P:glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000181">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002975">
        <property type="entry name" value="Fungi_Gprotein_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF369">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-2 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01241">
        <property type="entry name" value="GPROTEINAFNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="161"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="273"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="298"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="323"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="392"/>
            <end position="395"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="52936" checksum="D6BA89CEE2DF452E" modified="2017-01-18" version="1">MGICGSKENSAPVERKSQPVAAVRKNTSGDTTDTSGKGTTRDVVSSSAGSQEQTGIAAAATPAGKSDAHTNSQVPAPTTPTPTPTQAQTQTPAATTTPQTQPHTQTPAQGLPQQRQPSNSSSKLKSKQTLQTVNENVESKNTKCLTKPKTQAKVLLLGAGESGKSTILKQIKIIHQNGFTESEKLVYKPFVFQNICEAAKCIANALKEYDLISELVNVTSDDLEFIVNYEVPFEADNTEPLNPRVADIIDQLTADPTVTELFKNPDYDFYILDSAGYFFENLERIRQADYLPSVTDILRTRKQTSGIFDTKFQMEDLDIHLYDVGGQRSERKKWIHCFDNVTAIIFCVALSEYDQTLLESPTKNRLEESLNLFESVVNSMWFRRTAVILCLNKIDVFIEKLPHSPMEKYFPDYVGGANVNKAVKYILWRFKQLNRANLNLVPYVTQATDTNNIKLAFAIVRETILHNNLLDSGIITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI16</accession>
    <name>A0A1E3NI16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_36465</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016096.1">
        <property type="nucleotide sequence ID" value="XM_019163008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI16"/>
    <dbReference type="STRING" id="763406.A0A1E3NI16"/>
    <dbReference type="GeneID" id="30179695"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005364">
        <property type="term" value="F:maltose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106081">
        <property type="term" value="P:maltose import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106082">
        <property type="term" value="P:sucrose import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF35">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 45 MEMBER 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="56616" checksum="13B47F12E47A4EC4" modified="2017-01-18" version="1">MAATASNYQYQNNGQRQHLYNPQRSTAYIVILTLIVGALQLAWSTEFSEATPFLLSLGISKRVLSLVWLAGPLSGTIGQPIVGMLSDKCMLSYGKRRVFILIGCISTSVSLLALSHSSELVGLFTSPFDWDDRTTELAVICFACVGIYVLDFSIAIIQASSRALIVDVVPTGQQQIANAWAARMIGIFNLIGFWIGTLNLTELFPVLGDNQFKILSTLVSVPPQVKTVCYAQLFAWIGYFPLLFYTTSYVGELYLYEKGYPNPALIPPELKQDLLDQSTRVGTYALLSNSIVTLAVVTFLPMLLERIGTRSTFNEKLNLRTLWICSHVVFILCTLCTFFISSYKQAIALFAFTGIPWGGAVWIPFTLISEEISRIKDIIAIQIFYRQQSEGSSIAEDDAPEINSRNSIYCQKILIDFYDNIEYDSGILLALHNVFVSAPQMLSSFASSILFSIFHKAHDDNSYDSSLGWVFRFGGVMAIGAWYISRKVKTKEELYDMDRLMALSIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI78</accession>
    <name>A0A1E3NI78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_73332</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016950.1">
        <property type="nucleotide sequence ID" value="XM_019164450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI78"/>
    <dbReference type="STRING" id="763406.A0A1E3NI78"/>
    <dbReference type="GeneID" id="30181137"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000007">
        <property type="entry name" value="NAD(P)H-dependent D-xylose reductase xyl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="7">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="320" mass="36479" checksum="7481E46545E55390" modified="2017-01-18" version="1">MSTSNYITLNNGISIPQIGFGCWKVNKETCAQQVYDAIKCGYRLFDGAMDYGNEKEVGEGINRALKEKIVKREELVVVSKLWNSYHHPENVPKALNRVLTDLNLDYLDIFYIHFPIAQKFIPFEKKYPTGFYCGENGWEFENVPLASTWAAMEELVGKGLIKSLGISNFTGPLIDDLLRGCKIRPQLLQIEHHPYLVQPRLVEWVQSQGIQITGYSTFGPQSFVELDHPSVSLCVPLFENPVIMKIAERHAVSPGKVLLRWATQRNILVIPKSNKKERLVSNLSVNDFNLTDGELSEISGLNMNLRFNDPWDWCKIPLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIN1</accession>
    <name>A0A1E3NIN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_44509</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017103.1">
        <property type="nucleotide sequence ID" value="XM_019163120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NIN1"/>
    <dbReference type="GeneID" id="30179807"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000063">
        <property type="entry name" value="Nuclear elongation and deformation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057124">
        <property type="entry name" value="Ned1-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24565">
        <property type="entry name" value="Ned1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="870"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="1032"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="575"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="577"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="615"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="634"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="710"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1162" mass="129017" checksum="762E7D0439ADAF6F" modified="2017-01-18" version="1">MQYFGRAIGSVSKTWNSINPATLSGAIDVIVVEDEAGERHCSPFHVRFGKFQLLRPSQKKVTFVVNGETTDIPMKLNDEGEAFFVFKTDGYVPKHLQTSPVVSALSSPSDSPRMSPSNSSGDVLEYLDIGEGASDSNLLSTNTIKSDSELAKLRKDRLCKSPIERAKHISMKLNIPSKVEANGDIFLDMDGYKSDNKDVHGTDALVRQILEDEFGDDVDVQSMMNKDLKGNIRILNSTIDSSVPGTSSASIVSTPSANLSDNSSLQADAAISTASTTRSNDVHFKSLRLTSEQLKCLLLNDGENDITYSVNSGKSIVTCKLFLWKRATPIVISDIDGTITKSDALGHVLTMLGRDWTHEGVAKLFDDIEFNGYNIMYLTARSVGLADTTRSYLQGVQQDGYKLPMGPVILSPDRTIAALRREIVLKKPEVFKMACLNDIRALYYPESKGDGKIPEFDDDHLAAIVDESNTPFYAGFGNRITDAISYRSVGVPSSRIFTINPEGDVKMELLELAGFKSSYVSIVELVDQFFPPVDIGQGKDKKATDGSMEMSMSNAKNKYTDFNYWRSPDIDLSLISDDSDEAEEDDGSSEEEKDDAKSIGSRFSFFMRSPMSSPDKNRAVDAREDDERPSKAADDDDEGDDDDDSDSDYIYETSEDEDEDEDEDEDKDEDKGDDEDAGGDDDAQGPGEQDAAEQEKETSGSGKLAMGSMKNGSRSSSVSAVNNTFSPSSTLLSPQSLVSPNYPTHQLTSTSITSSRMKSPVGLSRFTLPDCSHLPVFKTRNYDEVLSIKNDNSIHLSTMLTPNEVNSLPDWLGGVIGNDLGSSVLTEKFNNLQIQERARLNHALTKTDIANAQSTGERPVISAGVELGRKNRYKDIFLYEHARVKLKKYDPASKELDSVASYINASYIHYPNSKLNYIATQGPLNETIGDFWNVVYFNDVPIIFSLTPQSENHVEKCAPFWNSGVYFSNGVKIKVDLIEEVEDLQLSYKTEAKCIARRILVTVGEKPPKEVLQLHMVSWPDYSIVICEEDILSLVGMKRFICKQLNIESVPILVHCSAGCGRTGSFCVIDTCIDVLFKEEKEHKQETKDLIYEITSQFRSQRVFMVQTLRQYMLIYDSIIKFVKMHEKAPKFVVGDVLTGEGLVDWERKDPGIIKRFIETYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIQ3</accession>
    <name>A0A1E3NIQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="2 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_73502</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017140.1">
        <property type="nucleotide sequence ID" value="XM_019164501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIQ3"/>
    <dbReference type="GeneID" id="30181188"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="511" mass="58212" checksum="EDE834E673D75C0D" modified="2017-01-18" version="1">MGQNSSKLSKATYVAATPSNDADDELIYKLQSLLTTEGSDEEAEDKDADDDDGLPQDLKNPMDVSDLKGWKDEIYSDPVKKLALNCMTGNDIDKIIANTQKINVHNVDLFNNTVKFEGHPITNQKSSGRCWLFASTNVFKEFMKSKYNLEDFEFSQNHLFFYDKLEKSNWFLNRILESYDEDIDSRYVQFLLQLPENDGGQWDMIVNLVKTYGLVPKAVFPDSASSINSSRLNYFVNNKLREYAMILRNLKREQLAQSEKGDSFKVDIVAIKKKMMKSIHTILSLTLGIPPGPNDPIVWEYKDKFGNYHRIESTPLGFYNEILGFKADEHFSLINDPRNTEGLYTVDKLGNIEGGKPVEYVNTSIDYLKKAAIDMIKANTPVFFGSDVGKFEDVQKGLLDVGSWDYDLGFGVQMELTKKQRLLTGSSQMTHAMVLTGVHLIDGKPVRWKVENSWGEYGEHKGYFVMTDEWFDEYVFQIVTNATFAGPKLTSIWLSKKYNVLPYYDPMGALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIX0</accession>
    <name>A0A1E3NIX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_54043</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017198.1">
        <property type="nucleotide sequence ID" value="XM_019163172.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NIX0"/>
    <dbReference type="GeneID" id="30179859"/>
    <dbReference type="OrthoDB" id="447173at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000235">
        <property type="term" value="C:astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902850">
        <property type="term" value="P:microtubule cytoskeleton organization involved in mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000996">
        <property type="entry name" value="Cytoplasmic dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1796"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2442"/>
            <end position="2595"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2784"/>
            <end position="2948"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3111"/>
            <end position="3138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3267"/>
            <end position="3315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3570"/>
            <end position="3597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="4064" mass="466602" checksum="A355BBC7A9317FAF" modified="2017-01-18" version="1">MDEVVKILEEISSSIYHKTLSYQPDDVINYVGLQECRVIYLTETENEVVAILNENDDRMLHSLSSTVAVIKRQATLDTSTSIRNQLILLSFPTSNTSSDSEFYIENLKSLVKYGVGSYFNILDQKHQLEGFSLHAIGNTKLKIKNMLLDIQGLQQTIQPPDLLLDIPDPIQKWLQSKEGKELSQALEESADLIEDVHFLNLVQSLVHTWVKNIQSIIQLDHNPLDGTVRDEITFWNAKESALNTIKTQLASPNVSLILDLLKISKRSHTAVALISSLSVKDSLKVATVNNNFLKNLTINNVYSAETISNLQLAIGDVFENFKKIKIIRYPIEKAVVLLRLITKDFESRLIDILSKYNLFQVDANEFESILQQIETQLDFFDNNVRAVINLLRGMLRKQENIFIPIKIETPNFIKESMHEIKAIRNDHESFNISIMSLVDEFESAPDRQLPICQQLYNESQVAYSSLSTLPLNKIFEKEGTYFNSHKTTYYQQISFVERKLVNVIKSLLAVVSNEPSALFVIFRKFNFLLSKPQIRLMLQQYYTILLNSTEKELDYLETQFIYQSQIKDILSLRHLPTFSTKMVWIKQALNSIAKIFSHLELILTKQWSNYPEGKRFSARISLLLSEIDIDGLLDAWSTETNKRMNNSILKSRVFQETTLKNGDPQYLISMDYADLEIGQELKCLKLLGMKIPASIMTYSGKISSIYKYASLFDELLRQFYDLHQKVSLLKDLAILVSPTIRDIISNIQYLSSQTWLDVSKSEEPIDAHTCENSRLVGSINQFQTNVSSLSDDLYKILEMKEEYYSHLQKIEHCVFDSRILSENIANFQKLIEKLVDQKSPNKVGFIDFVNGKLEQILAKKCKLEMERFHSQWTLEEAQIFHDVRTHKIVVHDLSISLSPEIQASKQYFLEYLNSLILSVKTQKPLFAPTYDKETIDRKFKFDGYELQASYKRCFEIVQSKIGECESFFAKWKSLQFLWDNEDLKLKFTASQDLELWLQILTKFREMREVFDTPESSKAFGLIKVDYQQAQNRIYSKFDSCQRSLLESYGLVLKSQLQIEAQELRKVKVESESQIFDLSNPISTISVLSSLVTNKHYLNSKLAVFNSLEKGQSILRKYRYKFTSDWIFFSQISDDIEGLKALDLRNLEYLNSHSDIVQAVLKEEIQNIIKITLATKTKWQKQKPDQKSSVQNAIMILQNFENSFNVIEMSYQKLHKACIMVGLSIDIDLSREFEEIKHLQYIWSYIMKLYNALDSIKTVEWFSCSINEVKSTLEDSLSLSRTLPVNIRSYPAFESAQMKIKEALKCIPIIRSLQNDVIKASHWIEIFEHIKLKKVPEVFCLGDVLDLDLSKNEKYIKSIITKAESEAVLEVTINGIEQSWATYKFEVYQMRPHIKLISGWSGLLSNIEDDINILESVKVSPYYSTFAHRVKNFESRLALLSQIINIWHEAQKQWVYLLGIFENKEQIGKLIPRDLSKFENISFEFNHLTSSVLSASVALDITDIPDILKNIQRLSDSLSKIRISLVGYLEKQREMFPRFYFMGNDDLLELLGNSTDSLVISKHIKKLFPGISLLNYDKTLMLIKSVISAEGEEVILKNPVSFNSAGLIDWLTSLDLSIKSTLSGLLIVLVEELNRLAYNLELAKVLELLSLYPSQIFILAFQIYWTKIIEKASSFGEFSSPIDELERLIKGLTNVITSDVSSLSRVKIENLIIELMHKQDIINSLKEENSSSDSFVLFCEQRFYLGEDFTNKTRNVQIIHGNYVATYSYEYLGAIRKLAYTPLLTSTFALLSEALDQNLGGLLLGPAGTGKTESVKALGHSLGRPVFVFCCDETFDVESVSRIIMGISQVGGWGCFDEFNRLEENILSGISTKIEKIQNALSTSSRKVELLAKSFVINKNTGLFITNNPNYEGRSTLPDNITSKFLSFSLMVPDSETIAKVILATKGFTNASSLAVTLVQLFDNMKNSCSVQRHYDFGLRSLKSVIVHAGKMNRSMPSNNEYNSELQVLQRSCYDVILPRLLPDDEQIFSAEISKFNLPYKPSNDYEDFKTILSETAKLKGYTATKSWLLKCMQIYEIQKINHGLILVGSSCMGKTVSFSCFLEALSRYSTVQNECYRIDANVLNKHSIFGELDYATREWKDGIVTAILRQASNNNKINKRIWIIFDCNIDPNWVENLNSVLDDNRLLTLPNGERITLPENMRIVFETDSLKHATPATVSRCGIVLFNKPHFAICDIFREALVNFKKNKVRNDDDAYDKTLISHNLTTHDFRESFVNIMFDLVDSSSLEHLSKLSKAFTYVMHVSDFKIISSLVNFMKQTLKRLLDFIEIHPHIARNNFDAFFKKETVQILIWSFASELTYDDREAFAKELLEIPNFHHVVPVSDAKDLLHAHVCLPGGDLENYNSELQEVDIQPDRILGPGNVISTVDTCIYEKLIFSTLDQHKSLILCGPPGSGKTMLLLSCIRRSVGFELVGMNFSKETTVDSVLKALEQSCEYKKNVNNITLGPKIPGKWIILFCDELNLPKSDVYGTQQVIQFLRQLVTENGFWHPKTRVWVTIENIQFVGACNPISTSGRNSMTDRFTNFCTTIMVDYPSLESLGRIYGVYSKSILKTIPDLTKYSDSFSKSLIETYVRYQTHFKDSLRPHYLCSPRELTRWVKGVYIALKPLISCSLENLIRLWAYEALRLFSDRLVLINEKSWFFDTMVDIAKTHFPHTNLSKALSQPILFSDWLSYDYQSVDKKQLETFLRGRFNVFNEEESWVDIVLYEDLLDHILRIDRVLKHVQGHMILVGPSGSGKTTLTKFVSWMNGIHPVQLNVSKHFSLRDFDEILKGLLFRAAVRGEKICFIIDESTILDDSFLEKMNTLLANAEVPGLFDGEEYENLISSCLSASRNEGLVLDSSEEVYQWFVQRIASNFHVIFTMNDNDETNPFISSPALYNRCVLNWMGNWSKTSLETVSREVTKGIPLDNSEYKIEETKGTFTGLRKVVTSLLVLIHTHVGELSGHITPSHFLNFLKTFSSIYTQKESTLQEMNSHVNKGLTHLKEAFLKVKRLGVIMAEKEEKLRIEDDKARELLNKMITDQNESERKQDMSMKMQQLFADQEKEIIKRREVVMKELNEVQDLIEEAQKGVLDIKKQHLTELRSMHNPPETIKLVLESICFMLGYNVQNWRDVQHVIRQDDFIASIINFKGDEQLTSEILLYMENHYLSKNNFNYESANRASKACGPLFMWIKAQLKFSAIVVKFSPLKNELEKLEGKLVDTKAKLIAINSLVKDLQDEVEKYKIQYSETIRVKENIRIEMENVRQKLDRSVKLLSSLGSERQRWEANVLDYQQQKETMVGNIILVSAFTTYCGFLAPTFRTKLLDAWKSLLSEKQIPFSTEEGQLLTTYIFPADNVIEWQNYGLPNDEQFIDNTAILASPFDNRFSFIIDPSELLIPFLIRLTEPKQLVITSFLDPNFKKKLENCAKFGGTILLCDGEYFDPVINRLIAKDVRVMGAGRTVVKIGDRDVDLSPDFELYIHTRDSTLEVSPFISSRMNILNYSFTSETMINEALNLTLEVRDPEVEAKRLLLTKKNTECKEQLKKYEKELLELLNSSEDNILDDDKLLSKLETLKQETKVLETQMIESASMIHSYNTIRNEFRALGELYAKLTTVFDKLVAVNKNYVYPKDELKKILRGSLEFKLSSTVETIITEFSQRTYRETSSSILEKDKGILKRLLDHFVGVANYEDMSLSENVRKNQIILLRSTKGNDATIRIKEISLGENVELVKYAMGSSDDCDVASRMIMELKGSKTWLVIENIEMSSEFLELINETISILKSGDIAKEEKFKLILTSKIESKLPPLLLKSCKQIVIENEQGLKKNMIDQLLNDTSPLSLHKLNNIKPKELKKLYLVLVWFFSLLLSRLRFSKGGFTKRYEISQSDLRNAEKFIGKFIISKYETDEVEFEVSAEMLETVGKMISSLIFGGKFDDHGDLQALQRCGFALFRSGMLGSHFNILEFGGSDKLSAPEGYNTMDYVEWIKNLPEKEPMDWLGLPEDAETTQREIIERKSTEAFQRILEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ12</accession>
    <name>A0A1E3NJ12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16868</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016681.1">
        <property type="nucleotide sequence ID" value="XM_019161372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ12"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ12"/>
    <dbReference type="GeneID" id="30178059"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="57"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="8">
        <location>
            <begin position="279"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="4">
        <location>
            <begin position="279"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="60799" checksum="661BFC1D22A79DDC" modified="2017-01-18" version="1">MTTHAIAGYDLPLDLWEKKFVAHDNANSNTITNSTPPIDLRSLTYVSPTETLDHVSCPICKLPFIEPWSTICGHTFCKECIFESFKSVLGERCPLDRVHLRVPKSIRRQLRLRKNSHNRTSSDSIVNLGEGGEDNGEDNDEERDSIDAFGDEENDIDIYPAPIIVSNMTDDLEVFCLNKSRGCTWTGERWSIKKHLDDTCEFTRILCICGEFCERKTLVENRLLTQSPSGLFILSLKDVPVDRKNNIATIEESDAYGGTFSEDDAESNPDIDADGIPICPHTCIPCADCAQKVRLMDITFHLENECLKHITTCTGCHLSFPLMHLKLHQKHCQKIYVDCPGLQYGCTWKGQRELLTEIHENDCVFIKMSGYFKKLEGNMQELNKENDSLRLQMSSILNSIVNGKVHNLGYPLELEEVGVMHDSGNSNLQHGTNIHGGTLHDMGENIGANFSFPAETQRMSMSKVKTLIRELEINKNVTKALVDENVNLREQMNSQRAMLLSLQQQMQFMMIERRRMFIGKNSSSSTSLGVEAKLTTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ31</accession>
    <name>A0A1E3NJ31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein SYS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17358</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017270.1">
        <property type="nucleotide sequence ID" value="XM_019161562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ31"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ31"/>
    <dbReference type="GeneID" id="30178249"/>
    <dbReference type="OrthoDB" id="542931at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF0">
        <property type="entry name" value="PROTEIN SYS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="20089" checksum="731B47728CE37524" modified="2017-01-18" version="1">MVWFSSLHSRYTSISGTDSSTSFNLTFKLAAQIILLQIFYYFTALIVFFITAKLLGWDFHIDWIYTWSNISVENSFGLTLMFLWLLNTLFSVILITFFIGRSKLVWDFALTIHFINFLVVSFASGFPKSVYWWLLQLVSVSVMMILGTYMARWIELRDTFFDQAEDIELGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ43</accession>
    <name>A0A1E3NJ43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ER membrane protein complex subunit 7 beta-sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016711.1">
        <property type="nucleotide sequence ID" value="XM_019164382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ43"/>
    <dbReference type="GeneID" id="30181069"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133382" description="ER membrane protein complex subunit 7 beta-sandwich domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="26894" checksum="5CF01E191FFFF8F6" modified="2017-01-18" version="1">MMHATLTLDRLVLWCWVLLQASCTAAAAVFELDLDISIADKSNKNHNHYKDLMHDPSRTYVTVYAVEPAAGSAAAGPADATLAYLSTVHADDEGVFHLSLPQGAGDRPADVFQLNFQSLDVFFAVSSQFRVHVAQSAATVQQHLPGLHPSLQSSLPSVLVDDATHAAVLDGALFRISLNHYLQQDAAGLAATVVRSIPFMSTILASRWMTIAFVSVIIVALLPAVLTRLDPDFADRLVEAQQEANQAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ85</accession>
    <name>A0A1E3NJ85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016751.1">
        <property type="nucleotide sequence ID" value="XM_019161400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ85"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ85"/>
    <dbReference type="GeneID" id="30178087"/>
    <dbReference type="OrthoDB" id="9992197at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12117" checksum="D15F653362E8D08B" modified="2017-01-18" version="1">MAIQQVSRVGGPNKCFGTFQKALECMTNENTKSNDECKAFIGDYVECRRHKLETYKMVIMQQHLEKDRSDLDAEKVGAPYVKKRDYLTPKTLGLVGGDDHAISTVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ95</accession>
    <name>A0A1E3NJ95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016761.1">
        <property type="nucleotide sequence ID" value="XM_019161402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ95"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ95"/>
    <dbReference type="GeneID" id="30178089"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140932">
        <property type="term" value="F:5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.40">
        <property type="entry name" value="Scavenger mRNA decapping enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008594">
        <property type="entry name" value="DcpS/DCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011145">
        <property type="entry name" value="Scavenger_mRNA_decap_enz_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05652">
        <property type="entry name" value="DcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028973">
        <property type="entry name" value="Scavenger_mRNA_decap_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102860">
        <property type="entry name" value="mRNA decapping enzyme DcpS N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35490" checksum="FFF48B54D3A9B949" modified="2017-01-18" version="1">MDTKTEPVTRFKYVRTLEEDPLAKRVVLLGSIDDEQAILTLEKTGFSQTVEPERLLRQVRTIASNDVYWWGTTLAEQDVEKDPTCKYSLVYPATETHVRKYESARLHMIRETPEAYQTVVKPYIETMKGDRLQWVTNILHHGAEAERVLFRNDDYVVLPDMKWDGQNLDTLYCCCIVYDDSISSVRDLTVGHLGYLERIRTSILEELPRIYQAQGLQRDNLRLYVHYQPSYYHFHIHVVNANFLGLANSMLAGKAILLEDVIDNLFQMASASASASDRSLGYASKTINYQLKETHKLWDLGLRDYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJE3</accession>
    <name>A0A1E3NJE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cu(2+)-ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_33366</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017344.1">
        <property type="nucleotide sequence ID" value="XM_019162816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJE3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJE3"/>
    <dbReference type="GeneID" id="30179503"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="729"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="771"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1126"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1156"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="14">
        <location>
            <begin position="282"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="14">
        <location>
            <begin position="360"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1254" mass="136484" checksum="3C194251FFEDFF0A" modified="2017-01-18" version="1">MSTSPLVPVKSPPYADVMDDASTSLPGFVAEEIIEESYGALNNGARTAVSAETGTSSEENDPNEGYLLPFNQTVAVHSFAIIPVELMKNRSVLGSSLANWFGSMYAYILTYYIPVYLTTVLDIPSEGVGLRLIPNIFATSLSSILSGYYMKWSGKYLRFSIIINAIGTHGIFQSSVTSVQYAFRSIGSTLGTSFSSYLFTTSLSHYLTVKLTSERPADVTDDMLQAIIHAASHDATYIRSKEAPVWATSLMIDSYNISVWYTFVLALVTSVLGLLAVCAIKENKLHTMGGMTCSSCSNSIKAEVAKIDGVESVSVSLLTEEATVTHAPSVTTEELIEKIEDMGFDANFIDNKLNSSSAIVTGTLKLYGLVDQSSADTVEQSISNLEGVLSCQLTVASEEMTVEYDPDVTGIRAILQVVNASGYDALVKNKLDSTTQIDLLAKVKEINYWRDNFFSLLKFGLPVIFLSHIFPIIRKSCHWDSNKMRVTNGLYWDIILQFSLGTYIQFWLGEKFYINCFKSLSHGSGSMDVLICTSTSIIYLYSVFSIIHGIFTDSYPVILFDTSTMLLMFVSLGKWVESKAKGNTSTALSKLLSLAPSSCVIVKNPEMFNKGRVTTLDSSLITQQQIGIDLLQKNDIAVILPGAKVPSDGVCVFGTSEVDESLLTGESLPVRKVIGSVLIGGSVNIISTIFMRITKLGEQTQLQQIVKLVKDAQISNAPVQRFSDTIASIFVPCILIFSILSLIFWTSYVEYTPIDSIPPIFLDSKDPSKVAYFKVLQVAISVIVVACPCALGLAAPTAVMVGTGVGATNGILIKGGEVLENASKVNCVIFDKTGTLTNGFMELTSFRFLGQYTESANFLWSLLQVIESNSEHPIAKAIVRCCNQELLNQNPYLFEISSVDTHAGLGISASCVDIETKRAIDVKLGNSKFLERFKILNTVEFEESLAHCSAKFRISSICHILINGAYAGYVELSDSLKSDAKTTIETFIDQGYSVGMVTGDLKDTSRHVANLLGIPLNNVLAEASPEHKLEYVKKLQELNFNVAFVGDGINDAPALVQSNVGVAISSGTDIAMSAADIILLSSTEESSSAEGVEEVNVSNNSHIGLLGVYASFDISSATFSTIKLNFLLAIIYNMVMLPIAMGLLIIPFSITMHPMFASAAMACSSISVVGNSLRLKRWSVTKLKRKMQNSADKSYNLGWNDGLPDIRGDINELSVDSFVANGNSRMTSVSFAVSRFYKSLTGLFRRRNQYTHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJE4</accession>
    <name>A0A1E3NJE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017343.1">
        <property type="nucleotide sequence ID" value="XM_019161104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJE4"/>
    <dbReference type="STRING" id="763406.A0A1E3NJE4"/>
    <dbReference type="GeneID" id="30177791"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019487">
        <property type="entry name" value="RAM_signalling_pathway_SOG2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10428">
        <property type="entry name" value="SOG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="836" mass="91178" checksum="F6B5D51F3D0DB8FA" modified="2017-01-18" version="1">MDTQTAELLHGSSSGSGQTLQQQQQYQQPQATRSRSTSESQRATRAEKRMGRLFRQQSSSAVNSHSKQLSDQGLNHHHAPHLHYPQAQNHPEALSNSHSISSVTSSINNSCNINNTTSSTATTNIVTGHANANANASTISNTAVNGTLNGAGHPSLAGSTTTSVAAQDDFIGPHDGQQDVNFAHSNSSSISSQVGSSTSSSTTPSISSNQTTRKFGFPLTGVGAISSPEIVSPNMYSNMSVSTIVTNPDSMVSQDESMKLKHRENSSKLSPLPEKVDISLDTLLSMSKKFVYFHRRFLSTLESLNINISLSKTNFNVYLAVLDNVERKFVQKGHEQITESMKAHLKASLVNSMKDLRKYIENLKLYFKSSNLDVTISSLRMSYFTLFSLFIELANICKIIAPFSSRNNHFSQNNQSVNVNNSSSSIRLNNSSISKNKPPFIINRVPAPNPQSSSQVASSRQASLSRQHSKLDRQDSDLSISSSQAAETSNSSVMEDEKLFDLITHTVQAAHVVYSQMNEAIAKSAIMIAENRNSETEDQINENEHEQTVGVNNIAMKVKELTNQCLASMEQTKNVENCLTIVKNPNFNSSSDTDVHRNIYEQTNLFLKSIISILAATKGAIEDIPALNEVRSSLSILTRATKELTIKLESSSLKQSVLNNTPSTTSLVDQPNLSSIPSMGNLTFDQQQQQQSILQGMQSPVLQQQMESNSIRRINKLRQIPEHNENIIGTMKQHIMNLELSAGEMFNQPPLGVTTPLIASIGPAVANAVLPIKSPLKTTSTVNLNSSFISNNSTIDGTLNGGNISNGATTPLYFGDGNMQAEYNPFDRLVPKGQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJF9</accession>
    <name>A0A1E3NJF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016596.1">
        <property type="nucleotide sequence ID" value="XM_019164717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJF9"/>
    <dbReference type="GeneID" id="30181404"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF37">
        <property type="entry name" value="LD47843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="223"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="629"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="697" mass="78080" checksum="5C3E5016E77F1EE1" modified="2017-01-18" version="1">MSSVSIPSYASSRKRSSSKTQQNFHPMNSTSISELTSLSSLEDNNHNGLSNGKGSHVGNGDIQESQISLNLNSNLSPQLSNPSSISSSSDVSSFPSSTASESQRSSKPPKPLSLITDINQKDHSNVVTPIVTQKLIPANQQPPKSILSRKPLPTDNTSLSKPKIITNNNNNITSSSSSASKSSSSSSPVPNLKKFVHVVRLISPEQPDEYLKVLNIPIYPDTLKIGRQNTPKTSNKITDGYFDSRVLSRNHAELFIKDNQLYIRDLKSSNGTFINDSKLEPYKDYKLSINDKIDLGTTLESQMAHKKITCIIKEFDYISLKKFDNMVEEINNKDDLITKKMELFNNTFDALLFGEIVDDIVLGIDDSNSCDLNLNDDLLDLIISDKSRSHTDSKADTKTKLKSSSKQSKLDTKFVQGLDLKPSQTPQDVVKKLITAVNNEYIQQQRLKEMNMFLKNYNNSVAGSHSTSVFKLYDKLLKNEKGNTDNVNGDRISNDNRNNTGKPMISTPPLSPTQKQLKYSRMEDQLKNSMAELDIARQHLSTAKSNESKMKKFGIENENLRKLLGSSESRISDLEEKLKTVSQTNEVKDSQLERLQDELQQLKKKQEQKEKQMKTEDLDCKEDVVYEEPEVRSRDDVRHTVDESTDKEESITNNVKQLLQELSSGIVRYSGWIILSVALVYCTGPISVKSITELVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJN8</accession>
    <name>A0A1E3NJN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_72442</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the actin family. ARP1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017477.1">
        <property type="nucleotide sequence ID" value="XM_019164198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJN8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJN8"/>
    <dbReference type="GeneID" id="30180885"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000188">
        <property type="entry name" value="Actin like 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="43909" checksum="42E17E8AB5585537" modified="2017-01-18" version="1">MAAMEELYNQPIVLDNGSGTIRCGFSSDELPRVSYSNILGRPKYHKIDYLPSDINPDDTFVGNQAQLRRGLLKLTYPIEHGTINDWNTLELIWQQLLLHDLMESNTLASQGNEVSLKEHTMLITEHPFSQRRQREKMCEILFESFGLGAVNVAIPAVLSLYSTGRTTGVAVDVGDGVCCVAPVFDGFALPGSMSRINIAGRDITRQLQIEIMKDGYCLNSSSEFEIVRNMKERIGFVNASATPVKLNSYNADDQVEKFQLPDGKFLRVQKDSLSRPSELLFQPQSFGPEEQSIQDALYKSIMGADVDIRGKFFETIVLSGGCTMLRNFGSRLLHELRGIDQEVKLKIYASPERKNNTFVGGSILSSLSTFNNIVVSKRQFMEDPALVHDLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJR5</accession>
    <name>A0A1E3NJR5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_18855</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017482.1">
        <property type="nucleotide sequence ID" value="XM_019162091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJR5"/>
    <dbReference type="STRING" id="763406.A0A1E3NJR5"/>
    <dbReference type="GeneID" id="30178778"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="241" mass="27844" checksum="EE8AF29E5D82A67D" modified="2017-01-18" version="1" fragment="single">SYVIGWVYVVGWGVANYPTVISNAQLRSVQGISIDYMYFNMMGFILYTLYTGMMWGSPVVKREFYEENGEWPLIRLNDLVFGLHNLFTNSLVLSQAYCWGFRRNDNQRLSSTAKVILAGVAAYVVGASAYIYRTQGLEPVAGCFNWMHLFTSLGLVKIGMSVCKNIPQILYNYHRKSTHGWPILMIWLDFGGAFLSFVQLLLDAWLVDDLWAIFDNKPKLFLAIQVFIADFIFFFQHYYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK06</accession>
    <name>A0A1E3NK06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017011.1">
        <property type="nucleotide sequence ID" value="XM_019161486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK06"/>
    <dbReference type="STRING" id="763406.A0A1E3NK06"/>
    <dbReference type="GeneID" id="30178173"/>
    <dbReference type="OrthoDB" id="444135at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001209">
        <property type="entry name" value="PRK00175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF16">
        <property type="entry name" value="SERINE O-SUCCINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="134"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="519" mass="58430" checksum="E1C5AFF5183D0467" modified="2017-01-18" version="1">MQKLNSKTLARISGRQASNIYSRYVRCISSSSSALKPRPTNISRAAAINEHIKPKSPSLSQMSFPCLEKLEQRTKNLEENLENGPEPAYSKITRENVEIYKSHEPLFLDYGGYLPQYEVAYETWGTLNSDKSNAILLHTGLSASSHAHSTEKNPKKGWWEGFIGPGKYLDTDKYFVVCTNVLGGCFGSTGPQSIDPIRGEPYATRFPILSVNDMVRAQHELIVNQFGIRKLHASMGSSMGGMQCLAYAHEFTDEVNKIISISGCARSHPYSIAMRHCQRQVLMTDPNWNKGHYYGQLPPHTGMKLAREIATITYRSGPEWEQRFGVERADPSQKPALCPDFLIETYLDHAGEKWCLEFDPNSFLYISKGMDLFDLGERNRTKASKSRYQSENIYFGEVVSETDLEYKSNASCGFVEPIKTKRSRRFTAEEAEEDLLRGLAKFAHKDVLVIGVESDILFPAWQQREVANMLSKGSKLKGGDGSNIKHVELSETDSFYGHDTFLLAMDHIGKPVSEFLNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK42</accession>
    <name>A0A1E3NK42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase family 32 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_131018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017620.1">
        <property type="nucleotide sequence ID" value="XM_019160015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK42"/>
    <dbReference type="STRING" id="763406.A0A1E3NK42"/>
    <dbReference type="GeneID" id="30176702"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49569" checksum="A4D1A69BD96C9503" modified="2017-01-18" version="1">MYRRLGNHAAEPGETGPGQTLKTSLRSVLVVAKTFRLLLVSIATVFVLCTVFMSTSVTSQMDALGLVSPGTANTTGYFSFYRGSPPSLNTKSMASIEKRLRYFFPFDAAGEIENNIWQLWKYRADNEKFPSKCFTHMERWRVANLEYNHNLITLDEAEQQIVDYFSYDMPEVVDAYYSLKDTRLKYDFLKYLVVFTSGGVYADIDTLDAKPLRYWYQSTLKPTKFMVGIDVDYNDVNWDILYNRRLTFSTKVFRAKSHHPFLAKLIARIVYTSYNSAEEIASIDWNQAYQNVDSNDEPLIQFTSASIFTDTLFDYFNDLNNPVVHRVARTEKDLIPEQIFGPETSSIFSYKLFTLARGPTQVDDIVVMPQLTFKGPTTGYHKGDLNSQSYDSEYDDENEKDFYYYARPLHFLSWDSLVQNEKDKQLLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK46</accession>
    <name>A0A1E3NK46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017638.1">
        <property type="nucleotide sequence ID" value="XM_019159810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK46"/>
    <dbReference type="GeneID" id="30176497"/>
    <dbReference type="OrthoDB" id="10528745at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="121" mass="13757" checksum="C367B3A4E6E1A527" modified="2017-01-18" version="1">MPPALTRVVYIVRPGPQTQACVWQIYAHVKHTLDSRKEAVAGAEGVDQQPRLVDFKVPRSTYFGVGSYINQVQLTFRTDSEQQRQQLADMLDTPLPEHEKFSFTRREGKPSSSKQQQTPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK50</accession>
    <name>A0A1E3NK50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017051.1">
        <property type="nucleotide sequence ID" value="XM_019164475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK50"/>
    <dbReference type="STRING" id="763406.A0A1E3NK50"/>
    <dbReference type="GeneID" id="30181162"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="1">
        <location>
            <begin position="38"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="276" mass="30634" checksum="0B0D0146C8378BBB" modified="2017-01-18" version="1">MAIDAKILKYGDESARQVVKKYHITSSRAPSCGRRANLVMIHGGAWRDPNNTCADFDQFAEYFDEFCNNAGGEVVVYSVDYELSSEQRGRFPDVIVEVLRALDTVRRDAGDAADFCVCGHSVGCTFITQLFEHARILEAADAPPALRAAAAELQLPRITRAVFLDGIYDIAALLQEYPSYAFFVRDEFGTEQRAVELCNSVGRPGCPPVYATLERVLVVHSRGDELLSLRQPDTFVRWLDAAGVASGRVEKVYADFGLHNHVYVNKTVAELVYKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK56</accession>
    <name>A0A1E3NK56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Polyadenylate-binding protein</fullName>
            <shortName evidence="12">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_16402</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017648.1">
        <property type="nucleotide sequence ID" value="XM_019161191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK56"/>
    <dbReference type="STRING" id="763406.A0A1E3NK56"/>
    <dbReference type="GeneID" id="30177878"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140693">
        <property type="term" value="F:molecular condensate scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008428">
        <property type="term" value="F:ribonuclease inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000441">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000648">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="45"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="133"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="226"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="329"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="15">
        <location>
            <begin position="533"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ46535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="631" mass="70689" checksum="9C5A26D6BF0E3FF8" modified="2017-01-18" version="1">MSSPDVNQLADSVEKLSVTENTESTPVTTTEESPAPESQQNEVLSSLYVGELDPSVSESELYELFSAIGPVSSIRVCRDAVSKQSLGYAYVNYQNRGDGEKAMEELNYAEIKGRPCRIMWSQRDPAARKNGAGNIFIKNLHPDIDNKTLHDTFSSFGNILSCKIAVDQNGNSKGFGFVHYDNADSAREAIESVNGMLLNDLEVYVGPHILKKDRESKWQELVENFTNIYVKNFSTEWTKEDLEKLFTPFGTITSLYLATDENGNSRGFAFINYENHQDAFKAVESLNNTDYDGKLLFVGRAQKKRERVEQLKQQHEAVKQEKMSKYQGVNLFVKNLDDSIDDEKLREEFAPYGNITSVIVMKDDAGKSKGFGFVCYSTPEEATKAINEMHQRLVESKPLYVALAQRKDVRRNQLSQQIQARNQMRMQQAAVQGGMGQFVAPVFYNQNFMPQVPAGIRGGAFPGGPNPMMMQQGGARPGQGMPNFNVGANGQPVPVYVQPVFNDYPQGRMQQQQRYYQNRPNQLPQQQPRKQGKDDNLAQIISQLPSDQQKNVLGNEIYQKIVDTNKVEDPNAIGKITGMLLSMENTQILASLENDDLFNSQLNEAINAYQSYKNEESSVPAESSEAPAESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKA0</accession>
    <name>A0A1E3NKA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin fusion degradation protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_42887</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017680.1">
        <property type="nucleotide sequence ID" value="XM_019163118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKA0"/>
    <dbReference type="GeneID" id="30179805"/>
    <dbReference type="OrthoDB" id="422728at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000837">
        <property type="term" value="C:Doa10p ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000839">
        <property type="term" value="C:Hrd1p ubiquitin ligase ERAD-L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030894">
        <property type="term" value="C:replisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900182">
        <property type="term" value="P:positive regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072665">
        <property type="term" value="P:protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.50:FF:000001">
        <property type="entry name" value="Ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="5">
        <location>
            <begin position="26"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="6">
        <location>
            <begin position="126"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="319"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="319"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="372" mass="41556" checksum="DF8ACA47C5C403EA" modified="2017-01-18" version="1">MFGGFQGFSGQPNQFFPQMVPIPQSFEDFFRCYPISMMKDAGNKDVANYGGKIFLPQSALHKLTMLNIRYPMLFKISTTSTDKSTHSGVLEFTADEGRCYLPEWMMDTLKINAGTVVKIETADLPQGNFVKLEPQSTDFLEITDPKAVLENALRNFTTLTVGDVIELNYNSHIYQIKILEVKPESSSGGICVIETDLVTEFAPPVGYVEPDYEKLKEENEKKKREAAISAPSLKGKGSMARQINYGGLLQEAADEATIKFKGDGFKLSGKKSTKPKSDAEKNETEDLDLSGPVRPLELPEGYLFFGFPVTPYMDEFEAAEKEQQDQAKEGEAVFYGEGHTLRANKKRKDKNKSHIPIKNKVRSPEAIIIDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKC8</accession>
    <name>A0A1E3NKC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl-CoA thioesterase II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72655</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017710.1">
        <property type="nucleotide sequence ID" value="XM_019164266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKC8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKC8"/>
    <dbReference type="GeneID" id="30180953"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025652">
        <property type="entry name" value="TesB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02551">
        <property type="entry name" value="Acyl_CoA_thio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="5">
        <location>
            <begin position="108"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase 2 C-terminal" evidence="4">
        <location>
            <begin position="293"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="45427" checksum="7A89419FE806B947" modified="2017-01-18" version="1">MSTAHSVDPDTKHMSGPETGSSVNSNNRLLRKYVTNTSSLSDDGMSSDGSSNTSISISNDDLADLDFSLDLNLELDTKESALEALLDLKRVASDTFTNRSELFVPYRGRGLFGGTMAAQSVLAALLYSNTYEKSWKPMSIHCHFLHSAKPHPALYYKVSSLKDGKNYCTREVNLFQNDQLIFRATVSLQAYQLEGSAANLRGQLSHNRAPPLIGKDIDHPDDMLTQEELFKIWSKKAMNKKHLEYLKGKNCEEEVIRCYNREPCIWKLPLDMFDQDLVSDEERHAPPSDRTLRYWVKTKDSLKNPSIFNWVAIAYISDYFYLSANMRLNLKEMFTTKFSVSLDHTIYFNTEIDTNSYFSYNVKNIKAGENRSIMFGEMFSAEGTLAATTVQEGLSVVFTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKK3</accession>
    <name>A0A1E3NKK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_27976</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017787.1">
        <property type="nucleotide sequence ID" value="XM_019162525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKK3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKK3"/>
    <dbReference type="GeneID" id="30179212"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106055">
        <property type="term" value="C:mannosyl-oligosaccharide 1,2-alpha-mannosidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900103">
        <property type="term" value="P:positive regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097466">
        <property type="term" value="P:ubiquitin-dependent glycoprotein ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF5">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="487" mass="56733" checksum="740B9096D347E1BC" modified="2017-01-18" version="1" fragment="single">TDKELFQSTFTKDHLGKLKAETKALFNYAWKKYMDFGYPFDEVSPLTCKPNKRDIWDEFNTVKNDALGNFSITFFDAMDTFIIMGDRDKFHECVYTIKNTYTDFAIDSTIQIFETNIRLLGSLLTAHLYAIDPRRGLVIPEYDGFMLKLAYDLGKRLVLSFSHREDNYKFEGDDVLKYFVFSYPRTNLLYGTKDVPFGLKNEQCTAGVTSLTLEFSLLSRLTNDTLFEDVSRRAVTDFWGRRTKFDLIPMAFDTYLRTFTDSITGIGASIDSFYEYALKYSILFDDNFFYEIWQDSYKALLTHSQNKVGIFTNLNVNNGLGATEWIDSLGAFFPGLQVLAGDVSNSIELHRIFFKLWNNYGAIPERWNFMPLRSEVLAKNSVGLEWYPLRPELIESTYHLYTATKDPFYLRLGEDFLERFRAHYIAPCGFSGSLDILNDRRQDRQESFVLSETLKYLYLLFDVDNVVQQGNMVFTTEGHPFWFDKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL32</accession>
    <name>A0A1E3NL32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable endonuclease LCL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Probable endonuclease lcl3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_136834</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LCL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017944.1">
        <property type="nucleotide sequence ID" value="XM_019160097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL32"/>
    <dbReference type="STRING" id="763406.A0A1E3NL32"/>
    <dbReference type="GeneID" id="30176784"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="11">
        <location>
            <begin position="52"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="228" mass="26392" checksum="45C6CE22323466D1" modified="2017-01-18" version="1">MSSREVTLYHPLVLLYGFGTSLTILGSYSLYARYFKRIKRAADIPAAFFRKRFLYGKVTSVGDGDNFHFYHLPGGVLGGWGWLRRVPEINRFRELKGETISVRVCGADAPERAHFGRPAQPFSDEALAWLRRYVLGRWLYVKPLHLDQYQRVVAKVLVLKWTGFKDVSEEMIKAGLATVYEAKAGAEFDGKEAVYWRQQERAQHKKLGMWSINKKDFLTPREYKNKYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLD9</accession>
    <name>A0A1E3NLD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_16744</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018057.1">
        <property type="nucleotide sequence ID" value="XM_019161328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLD9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLD9"/>
    <dbReference type="GeneID" id="30178015"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="355"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="611"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ46944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="680" mass="76616" checksum="E9CF1450E0494E05" modified="2017-01-18" version="1">MSNWKKLFKSKTSGDEHQPSSRASADYSSDDQRAGNENNNRNSGSYGHNYSREASKTPVDGQATFSDSRKTLASSDLSKISSGVDKLNLSEENREPVLITKNLENTDPVIENDDEVSTSAGSSYGMSLNQQNSSNRMKPGLLTVKVYSSKDLEIPVPLRINKQILSKLASLGQHVNEEELNEKIDALNESLDEAANTNLSYYLPSTLRINSSAEPNKNVIAQYNLVYAIIEVENSSERISSVGNTIAHTKFNSVTTFDVTSPNNAILTIQIYIRIPGSLLESNKQEDHLIGSIMCKLSDLNRNSFDNLRPVRLLNHQWINLKNPYTDDDLIGSLNVTLDFKPLTKESKPLSIEDFDLLKVIGKGSFGKVMQVRKKDTGKIYALKSIRKAHIVNKMEVTHTLAEKFVLSRVNSPFIVPLKFAFQSSEKLYLVLSFINGGELFYHLQKARRFPLIRAKFYICELLSAIETLHSMNIVYRDLKPENILLDYQGHIALCDFGLCKINMKLDQKTNTFCGTPEYLAPELLLNKGYTRVVDFWTLGVLLYEMLTSLPPFYDEDVSIMYKKILEDPLIFPSDMDSVTKDLISKLLNRDPAQRLGYHGVSEIKSHQFFKDIDWVKLTNKGYIPPYKPQVKNSLDISNISSEFTDERPMDSVVDDFLSESVQKQFGGWTYVGSFGGSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLF1</accession>
    <name>A0A1E3NLF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydantoin racemase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HyuE racemase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017286.1">
        <property type="nucleotide sequence ID" value="XM_019161573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLF1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLF1"/>
    <dbReference type="GeneID" id="30178260"/>
    <dbReference type="OrthoDB" id="412018at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052186">
        <property type="entry name" value="Hydantoin_racemase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053714">
        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047:SF6">
        <property type="entry name" value="CN HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="29545" checksum="A7CCFE5173684CFA" modified="2017-01-18" version="1">MVVPFNDKLTLLPHAKGSCIGLVEPNKVQKILLINPNSTKLMTTNCIKMVEQYVPPDAIVYGYTAPETAPTTVEGHLDGVISSADVMRDAYSLIAQADACLVACFSEHPLINCIREEFDIPVCGIMEAAIYSAKIIGGRFGIITTVYRSQIRHEDGVMNYGISNGCAGLLSTGLKVAELHTKPREEVLEIMRNVATKLITEKDADTLLLGCAGMTDMKLAVEDAVKPYGVIVIDGVVSGVNILSGLVRSGLKTSKRGLFCSSLESRTARGQNWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLG3</accession>
    <name>A0A1E3NLG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_72980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018073.1">
        <property type="nucleotide sequence ID" value="XM_019164351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLG3"/>
    <dbReference type="GeneID" id="30181038"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044182">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006109">
        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11309">
        <property type="entry name" value="14-3-3_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="4">
        <location>
            <begin position="5"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="255" mass="28714" checksum="323EE681B06B3D1C" modified="2017-01-18" version="1">MTLSREDSVYLAKLAEQAERYEEMVENMKAVASSGQELSIEERNLLSVAYKNVIGARRASWRIVSSIEQKEEQKGNESQVKLIRDYRSKIELELTNICDDILNVLNEHLIPTAQSGESKVFYYKMKGDYHRYLAEFAVGDKRKGAADASLEAYKAASEVAVTELPPTHPIRLGLALNFSVFYYEILNSPDKACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMSEAGQEDANAAPAEKQDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLY2</accession>
    <name>A0A1E3NLY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_57711</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018268.1">
        <property type="nucleotide sequence ID" value="XM_019163253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLY2"/>
    <dbReference type="GeneID" id="30179940"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000188">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF50">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00840)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="36"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="482" mass="53356" checksum="E3BEAC9B82FD5CD7" modified="2017-01-18" version="1">MSNSSKQQGTVESEVDDYTIDPKIERAICWKFDLRILPLLAIMYLFNALDKGNISNAKTDGLDKNLGIEGQQWNNMLSIFYVPFVLCAFPLALVIKKYNAANIIPVLMFTFGSISLLEATAFNYGSLMAARWFLGICESAFFPGIIFYLSTFYRRHELARRLSIFYSAANIANAFSGLLSYGVFQIKNSKLHGWQILFLIEGAATVIISVFAFLYLPRSAATAKFLNEEEKEVARKRVELDSSAIQTEKLSIRDAIKVFKHPVCVAWMLQEILIGVPLNSINNWFPQIVGSLGKGTVMTNLYTVAPNVWGAVALIILCFMSDLTRVRSVFIVVGLAVTLIGFVVFGCIDTKTHIGAAYFSCFLMTTGGSVSSVLTSTWYNNNTPNENRRAVISAVGVPLANAAGLISTNIFRSKDAPKYVPALGITAGFGGAGILMVIGILGYMIFDNRRRNKNQGVSLTYRDVPTSELYDGPSNPNYRWMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLZ0</accession>
    <name>A0A1E3NLZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 74</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71207</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018199.1">
        <property type="nucleotide sequence ID" value="XM_019163862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLZ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NLZ0"/>
    <dbReference type="GeneID" id="30180549"/>
    <dbReference type="OrthoDB" id="2189106at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060304">
        <property type="term" value="P:regulation of phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000004">
        <property type="entry name" value="Probable VPS74-protein involved in protein-vacuolar targeting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF2">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3 HOMOLOG SAURON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="39992" checksum="15B3EF4DA4F04052" modified="2017-01-18" version="1">MSSGLSRRRGGAKKTSDNGDDEEASLENGVSNMSLNNSTNKESIRNPESKIGYDPRDDPSMNNEDKYGTKPFLTLLEEVLLLGLKDKEGYLSFWNDNISYALRGLIIIELALRNKIRMVNDPARKRFELADRLIEVVDSTMTGETLLDEALKLMKNDDENLSVLNWIDLLSGETWNLMKINYQLKQVRERLAKGLVDKGVLRTERKNFLLFDMPTHPINDPLPKKKITARVLNMLTSRNVILDYDEKYFPSTLEWKYLRTVVLVCGCSAANVLENVLVDVNFDMRDQAFIRAEELLGNFSDFPFIDKASSVGFGMNLNSEIDREVDAHQGFEMNLEVVASVISIFSKMDAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM36</accession>
    <name>A0A1E3NM36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial inner membrane protein COX18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_31167</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018300.1">
        <property type="nucleotide sequence ID" value="XM_019162679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM36"/>
    <dbReference type="STRING" id="763406.A0A1E3NM36"/>
    <dbReference type="GeneID" id="30179366"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="38201" checksum="6805F253517C856C" modified="2017-01-18" version="1">MLPRIYRPKKIIGCSRSYNRLQRRNFSFEPVSEGLKHASEMLIQVHADSLLPWWVFIPLATFTMRTCFTLPIAIYQRRGLRKQNELRPIIQATTPVLRAKLASVAQAEQIKRQKEIARGIKSPVPNEKATLTADKIMILAAKEKYKRQKALFRQYGCQTWKMMVLPAVQIPLWVSFSYMFRTITGWTDFGNKSNIDMSLTDEHFLHLYDLSVSDPYFILPITLGVVALCNAEWNFKTADLMQLTTRGKKNTLRPTAFDVIITLSRASLIFLTAVATQAPAALVMYWISSNSYSLLQNILMDRFLPLRYSPYERFTRTGSLPKDAKPLVRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM64</accession>
    <name>A0A1E3NM64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho GDP-dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15201</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018330.1">
        <property type="nucleotide sequence ID" value="XM_019160711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM64"/>
    <dbReference type="STRING" id="763406.A0A1E3NM64"/>
    <dbReference type="GeneID" id="30177398"/>
    <dbReference type="OrthoDB" id="1683373at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000004">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22467" checksum="E76DE2773C3BE94A" modified="2017-01-18" version="1">MSDEFKDLQPETIPGFKVGEKRSIQEYTNLDANDESLNKWKQSLGLNTGKALDVEAGDKRKVVIISMTLVIEGEQPVVINLEKEKNYDSLKGKKISFKIKEKSVYKLVIRFKVQHEIITGLKYLQGVKKAGITVDKIEEPMGSYAPNTVDKPYYERGFEEVEAPSGFLARGSYSAVSKFVDDDRTEHLILPWSFQITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMC7</accession>
    <name>A0A1E3NMC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Threonine/serine exporter-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_30721</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThrE exporter (TC 2.A.79) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018395.1">
        <property type="nucleotide sequence ID" value="XM_019162650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMC7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMC7"/>
    <dbReference type="GeneID" id="30179337"/>
    <dbReference type="OrthoDB" id="413008at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051361">
        <property type="entry name" value="ThrE/Ser_Exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082:SF4">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/serine exporter-like N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="427" mass="46612" checksum="D0756E5FDAD29361" modified="2017-01-18" version="1">MLYGAPTHRLEEYMSMTAQVLEIDGSFIYFPGCMLVSFGDLNARTSEMKLVRCAQGLNLGKLDEVHDIYKNVVHDRLGTVEGYELLDEIMNRKPKFNAWWCILFYSVASVAVAPWSFGGSWIDLPICFGIGGIIGFLQFVVCPKNTLYSSVFEVSSSIIASFIARAIGSINGGNTFCYAAIVQSSLALILPGYIILCGSLELQSRNIVAGSVRMFYAFIYSLMLSFGLTLGAALYGWLDKNATSATSCKSDISPWFYFLFIPLFTMGIALTNQASWSQLPMMSFISGAGYVVSYFSSKHFKEVTELNATLGCFIIGLVSNIYSRLLKSFNKYFTTRGTFMTVSLMLPGIFVQVPSGIASQGSVFTGINTANSLVHSNSSEAASTSNTNSLSFGMVMIQVALGISVGLYLSTILVYPFGKKNTGLFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMG2</accession>
    <name>A0A1E3NMG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methionine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="2">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_15288</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018432.1">
        <property type="nucleotide sequence ID" value="XM_019160751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMG2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMG2"/>
    <dbReference type="GeneID" id="30177438"/>
    <dbReference type="OrthoDB" id="24670at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.220.10:FF:000002">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="METHIONYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="11">
        <location>
            <begin position="10"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="546" mass="63411" checksum="43B5B62273E2AE08" modified="2017-01-18" version="1">MKPLNLKTIYTTTPIFYVNAKPHLGHFYSMTLADVRNRWCKLNKKDTFFTTGTDEHGLKVQNAAQAANVDPKLFCDNLAAKFKELAVVGNIKYNRFIRTTDPDHYAAVNHFWNVVNKNNFIYKGRHSGWYSVSDETFYAETDIEESTDEKTGEKIMISKETSSTVLYQSEDNYFFRLSAFQKQLLQYLKENPDFIQPRKYYDFIVNELETRELTDLSISRLSSRLQWGIPVPNDDSQRIYVWFDALINYLTSEGYPTAAEMPRPTHLIGKDIIKFHCIYWPAFLLAAGLQMPKQVFIHGHWLMGGRKMSKSRGNVADPIEIADRYDADSLRLFMMRYSVLDTDGDYNEERLNTLRSEFIDKFCNLMMRSLSKNFKIERALERLQHESLESLCSKLDEPQFQKSLESIRKDINCLKIDIDDDITNFNMHKALSKIFSTAPMINGLFDSYEPWTLKVKSSDAENDKESKTLKQDLIIFTALDSLRVILILLEPFIPQYSALLLDRLRVDKEKRTIDYAEIGKDLTYGKDAIFKKGSTVPLAKIEFSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMH3</accession>
    <name>A0A1E3NMH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_109306</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018369.1">
        <property type="nucleotide sequence ID" value="XM_019159731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMH3"/>
    <dbReference type="GeneID" id="30176418"/>
    <dbReference type="OrthoDB" id="195498at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF16">
        <property type="entry name" value="PEROXIREDOXIN-5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="4"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="184" mass="19527" checksum="FABB171EE2CF0F2A" modified="2017-01-18" version="1">MAEVSVGSTIPDIPVYYLPPTVGDVCVYAPSKININELEHPTVVVVVPGAFTPTCSEKHVPGFLTTSAIGHLKDAGVKNLLILSTDSPFITRAWGENLSSGKKEILKELQDGYIKFVSDASAELLTKLGLVGEPNDLFAKYGLRGLRSALIIDSSGKVKYVGVEKVKGAVSDSGIEAVLDALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMU0</accession>
    <name>A0A1E3NMU0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018557.1">
        <property type="nucleotide sequence ID" value="XM_019160805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMU0"/>
    <dbReference type="GeneID" id="30177492"/>
    <dbReference type="OrthoDB" id="3992150at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="41975" checksum="CE86C7316F35A78E" modified="2017-01-18" version="1">MFAKRGVKAFSQMRRMATEAGAGDAPVSNGKMESLLEKLASITSNGPAGAAEETMLGKAKGKNSRGNRKPNNANNQRSAKGNRKDASSRNNNRNNRPSRNNSSERNNQRRGTGRRPHTVQTVSHFDRFKNPKKSSTASQREDGLLNAAGLSFADMQEARALFIRRKVSDGGAATLHTGDAAVFNFFHSRAVHRANNYGPRADQFARFEETPNVYRAAEVSKPDLELSKDKMGYDTKSRVLRALEQITTRRGFKLSDVERKNDSFLPATALLYPYANTTLPNNLERPRANLKHLASVPEEEISATLAAVVRGQRPELVYNPKEDFKTEQLKINAQVVVNSLNRNAQLQVDNLHKSMAKVMLGQQPIKTLPQPLFPPKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN07</accession>
    <name>A0A1E3NN07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hpc2-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_57944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018592.1">
        <property type="nucleotide sequence ID" value="XM_019163265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN07"/>
    <dbReference type="STRING" id="763406.A0A1E3NN07"/>
    <dbReference type="GeneID" id="30179952"/>
    <dbReference type="OrthoDB" id="5576775at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014840">
        <property type="entry name" value="HRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08729">
        <property type="entry name" value="HUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hpc2-related" evidence="2">
        <location>
            <begin position="296"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="491" mass="52393" checksum="EFBAD08F689F9AA4" modified="2017-01-18" version="1">MNTHKTLSISSLLSTDDEPGRGANANPFPQMVSGANARFQSPPTTIPQSLNVPQAPILAVPLEPSPIKSMRIIAITDHSRKELSPMSVSNLMNSTNDEPVAYKERSPPTVVNTAKEPTAVLDAAGNNKPKKAGRKEPSVVSDTANSNKKDKGKKTDEAKENEPNEPLIITIDIPLSTHNDVHMEHNFAKLVEEKYGQYETQAPFTKGLWNFDGDGDAEGDDEGDAEGDVEEDEEEEEDADLDGETGPPSAAPNEYEEEDDIVKALQIKFTPGMSDMEKESLVLKEIHRRKMVNNKRIGKYDVDDPFIDDKELEFEEETGANADGWFIWHGKLDVSKKKKNSAPEPRVEKSARSSKASSAAGNSGSSRSRHNASSNNDNASNAVSTPSGSATAEKRRKAFDSSVVSSPAAKRPKKTASPPVPDSSAASSASKASAATPSTTTITTTETVASTATPTATTTATITPVPQTTNDPADGSKGSNKIIIGSFGFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN47</accession>
    <name>A0A1E3NN47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_32519</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018639.1">
        <property type="nucleotide sequence ID" value="XM_019162773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN47"/>
    <dbReference type="STRING" id="763406.A0A1E3NN47"/>
    <dbReference type="GeneID" id="30179460"/>
    <dbReference type="OrthoDB" id="2018507at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000441">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF57">
        <property type="entry name" value="AUXILIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ47526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="316" mass="35507" checksum="491F14A11599E868" modified="2017-01-18" version="1" fragment="single">MNNQQQPDLYQPGTQLTVGSHKVVVNKYFSHGGFAQVYTCTISPPWNNKQVACLKRVNVPDKGSLNTLRKEVDAMRKLQGINCIVSYIDSHAARSNSGNGAGYEVFVLMEYCSRKGLIDFMNTRLVNKLTEPEILVIVQQITEAVAHMHALNPPLIHRDIKIENVLLGDDGTYKLCDFGSVSLVINPPTNVEEFQVIQDDIMKNTTPQYRCPEMLDLYKRQPIDEKSDVWALGVFLYKLCYYTTPFEQNSINGNVNGYGGDYAIINGIYSFPSLPPYSARLKNVIAKLLTVDPILRPNTFQLLDEISKLRGVPCPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN55</accession>
    <name>A0A1E3NN55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_72896</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017976.1">
        <property type="nucleotide sequence ID" value="XM_019164323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN55"/>
    <dbReference type="STRING" id="763406.A0A1E3NN55"/>
    <dbReference type="GeneID" id="30181010"/>
    <dbReference type="OrthoDB" id="10258955at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009442">
        <property type="term" value="P:allantoin assimilation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="60"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="544" mass="58717" checksum="41515355A8927D5F" modified="2017-01-18" version="1">MSSAISSTRVVLDGAVGPATIIYSQTSGKIVAIVPSVLDAEDPLLSRYDVESYRELAPLVIMPGIVDSHVHLNEPGRTDWEGFASGTQSAASGGVTTVIDMPLNAIPPTTNVKNLNIKLESAKGQIWVDTGFWGGLVPDNIADLVPLINAGVRGFKGFMMDSGVDEFPMVTPAYIEQALATVQGKSTMLMFHAEMDGAKDGDALDNDALGQEQCCANHGHSHQTYDGLTSSQADALSKSPVLHAVEPLSGNISKLINKEASANIDDQLDDITPLELATKNASVLETVDPTAYSSFLASRPDSFECNAIHHIIKCLTKQPTTKVHIVHLATHEALPMIKHAKQTLGLPLSVETCFHYLSLRAEDIPNGATQFKCCPPIRNDNNRRLLWDALKNGLITSVVSDHSPCTPELKGLERGDFFEAWGGISSVGFGLPLLWTVGREEFGIDITDIVQWCCVNTAEQVGLSHRKGRISVGYDADFAIFDSEMTYTVANARTFFKNKLTAFDGKTLTGRVVETILRGNSIYALGKGLTEVPLGNLLLEPRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN69</accession>
    <name>A0A1E3NN69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tuberin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_76628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018677.1">
        <property type="nucleotide sequence ID" value="XM_019164950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN69"/>
    <dbReference type="GeneID" id="30181637"/>
    <dbReference type="OrthoDB" id="19311at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024584">
        <property type="entry name" value="Tuberin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11864">
        <property type="entry name" value="DUF3384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tuberin N-terminal" evidence="1">
        <location>
            <begin position="65"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1089" mass="126479" checksum="BACD7587FE61A27E" modified="2017-01-18" version="1">MGSGTTSGSQDRQSSVRRAFRSLSQVLGISSSQFEAQKIVSYQDGKFVVDNEELMEVLENGTTGDKVSALKLLRDEMVNITCEERFSLWKSVKAKINLKKEGVVRTEALKVLQELLNFSELPKTNGMEFYIDICESVNFMGIDRDMKIFLVCFHELLRFANITSFEKYTDHPLNDFLIKLHKQILMYRSEELDVLIVSLISECIEIDRHLFTSDDLQDLLNTVIDMSIKTKNMLELKSFLDFFDSYLLTGYKYEEELYTIFSILGCANGLEELETSGRCELLMDRILVDDLSSKVPFTLCDVIIARFDTQQRHRSGNRPIIGCLRLLSYTLKYFDEESRGGKAITSFFEHHLNYLFQALITVSERTDEAISIEVLKLIDEVLEKPFMLSGYYKYFIERNEFWKLLQLLNWRNKQPISSSYKDVLNELFNKLQNFDLNSFYVSRLIEYLEENYQILSSYNISYALNYYSKNLVCVCGALNWKQKCETIVEKYYSTTPSDVLKVLKNSFLYCIPLKIDQPSLDFYINILCYVCVLGLNFKLEDDVLEPLTDVILKLDDQMLEKVVDDYLMEIQSFTDLNKEIIAKTLILATLKFADNKKLSGKAYILLNSVVKITKLSAAHESLKLFNLAILLLTRIRARNEASKKHFYIYEIKSEDVRNEFFLSRNEKSETEENNKDIDVKLKQLLDAQLSDPRFEHILSQYFFDGKTKEFDTEIDPKILLDLYTDILRNTTSWEFYLLVVKHIRYQLLNYDLFRQICNESIIELTNLLTQQMQFIYTFKFERPHWLQTKHIRIAIVEVLYGIYPYKETIPLRQSEDLIRSVALDFHFFDIIRTPFLNYLHCCMFESPKLMRHFVYPILKMIQDDLSKPETLFSSLGFVLDLYELSDCYKLKQKEIKIIQDVLSRVINYNIERHDLINKEIKLSKVLAGLILKTFGSADENGVEPMVTQKMRILFTHLVCGRAGKEEELFARMVQCCKDKDSLAGSVVDLDKIEVSAAVCGVGTDAYCCISTGKDKIEVVLVEQQLSPQLVDESMGKKILITTVDENRDYFTVSSYGFEYIKLGSVFVGRNNIVQFVKVLIELCSVSALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN83</accession>
    <name>A0A1E3NN83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Exoribonuclease phosphorolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71609</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018664.1">
        <property type="nucleotide sequence ID" value="XM_019163964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN83"/>
    <dbReference type="STRING" id="763406.A0A1E3NN83"/>
    <dbReference type="GeneID" id="30180651"/>
    <dbReference type="OrthoDB" id="2504340at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="37"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="260" mass="28653" checksum="11C256875C79F52F" modified="2017-01-18" version="1">MNVIDRRRRLGPATAIPLTFPSAEVKLSQETEKKDNKLFIQLSNIPTATGSSFIQSGNNLLLASVYGPRPSFKRTFNSKAVLKVTFHSSPFLSSSQATGNDSNNSSSGKKHVGDEFQIVNSVISSTLETACSNLILLDNYPKSTIDIFVNLISTDTATKFIELLPLIHNAVNLALVDSGIAIKGFPSAIISENNIFINSIHSDTYDQNQHEDLENEELLTFYIPHLPSNDEKEFKLLLSDAIGQSRSLRSELSQFCCDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN86</accession>
    <name>A0A1E3NN86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_71629</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018685.1">
        <property type="nucleotide sequence ID" value="XM_019163969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN86"/>
    <dbReference type="STRING" id="763406.A0A1E3NN86"/>
    <dbReference type="GeneID" id="30180656"/>
    <dbReference type="OrthoDB" id="238316at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000028">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040725">
        <property type="entry name" value="PheRS_DBD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18553">
        <property type="entry name" value="PheRS_DBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="224"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="495" mass="55980" checksum="710E7F3E5CA508A3" modified="2017-01-18" version="1">MSDLQLNILKVLGAKGSIPDSTVEFPNEDSQQIQAALNSLLARAMVSYKTTEHEVYKLTEEAEDIVQHGSHEYRLLNKVIESLEGLKVSDVDSVMGKNGKVAQGRAFKNCWIGKEGDKIIAKVGSPAEVDDSTALQLKEIVKTGTLSNKKDLADLKKRKLVVPTKFFSFAIEKGPEFALEIKQFETDITADMILSGSWKSASFKPYNFNSEGDLPHSGALHPLNKVREEFRQIFFSLGFSEMPSNQYVESGFWNFDTLFVPQQHPARDLQDTFYLKDPATALPPSDKEYLENIKAVHQDGAFGSIGYRYPWHLEESLRTVLRTHTTAISSRMLKELAKDPKPTRLFSIDRVFRNEAVDATHLAEFHQVEGVFADYNLTLGDLIGFMQQFFEKMGVTNLRFKPTYNPYTEPSMEIFSWHEGLGKWVEIGNSGLFRPEMLEPMGLPKDMRVLGWGLSLERPTMIKYGVSNIRELLGHKVNLDFIENNPAARLDDDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNG3</accession>
    <name>A0A1E3NNG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_58058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018753.1">
        <property type="nucleotide sequence ID" value="XM_019163273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNG3"/>
    <dbReference type="GeneID" id="30179960"/>
    <dbReference type="OrthoDB" id="206213at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008413">
        <property type="term" value="F:8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015938">
        <property type="term" value="P:coenzyme A catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF24">
        <property type="entry name" value="PEROXISOMAL COENZYME A DIPHOSPHATASE NUDT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="32"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="317" mass="36901" checksum="051BB821D06EE9B9" modified="2017-01-18" version="1">MESKYLTNLINYKPRFFQDQTKFSVWHKVPVTRRSAVMVLLFLGKEGELRVILTKRSRKLKSFSGHVSLPGGKAENGLESEFQCARREMDEEIGLSRHNNILKREFGFVLDELKTMPAYLARTFLAVAPCVGYINWCEDVQQTERRISNLVLNPGESASVFSVPLRDFLQPRTEDVQLRECLKQSHIRTKWAGLPWNLRSFIFPVFNEKETDWLQEVEDLSSASEDETHEDQELDLRTRNCWGLTANILHDVAEVIYHGKESVAGEEDLIWSLRNHGQIHTKERTEFEKRLINNTKGSMFSEVIPQEEFAHLKQIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNH3</accession>
    <name>A0A1E3NNH3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018769.1">
        <property type="nucleotide sequence ID" value="XM_019160880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNH3"/>
    <dbReference type="GeneID" id="30177567"/>
    <dbReference type="OrthoDB" id="3995902at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="628"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="722" mass="80864" checksum="7520DE843F58C542" modified="2017-01-18" version="1">MLDKDGIPLLATQDPTLLDGGDLPKEKIVYTPEQLLTFEDKGTIPEGCELPDRSFYRFDATVLMKINSYLQNVNCSSYGSKKNGKRNGHRTKDAHHLYSSSNSGHSHGYRAEEEDNDEPEWMAETDFKVDRDFQLYMKAGSHTADDFEREKKLFRSKNSVVDAESAKAVNTSESSSCNTSDDSTAAGGDSNETNDEKLKRELLKQLEEPEILGPTEQEYYQLLAEKEKQKEEEAKAKAQKPNVSSGDFQFGLEMDSKTQVDNFFTSLLNKNTENAAQRGSSEDSTEATIQDTRNSRNTAPSTIAPGRTPLQRTGSQILPPGLDTTSKLGQIQSQASPMKETRGQENGRNQGLSPSQPMPIPGMFPPGMLPHNQFNQLNHNSYYGGNQQSPSDMQVQQMKQAQMIQHMQQVQHMQRLQQLQQMQQQPRSNFHSMGPNMPPNMPLNMPLNMPQNMPSNMHPNMHPNMPPNMPPNMPQNMPPNMPQNISQNMPQNLPPNMANMSNLSSMLPDGPNGNSNFSNIPGSGIPNMQQRPYLVHRSLPPGKLDQLPPHIVQQLINSQQHSPQPPQGMQHPLATLGNIKMNHNMNMNIRNQQLPQSPQENRQGSLQGSNQPTLRQLPHNNSGNSIAPQMPTQMHQQTTPQMEQHIPPQMMPANLPPPDQLPSPFKEKFYQIQQILLQCQQNGQPPPPALLHDMMEFHTLLEKNFSRSATGSTNSEGHTISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNR9</accession>
    <name>A0A1E3NNR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_10197</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018286.1">
        <property type="nucleotide sequence ID" value="XM_019159646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNR9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNR9"/>
    <dbReference type="GeneID" id="30176333"/>
    <dbReference type="OrthoDB" id="3986994at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF90">
        <property type="entry name" value="CENTROMERE DNA-BINDING PROTEIN COMPLEX CBF3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="792" mass="90530" checksum="8ECCA759FFC0F7E2" modified="2017-01-18" version="1">MPSTSNSSSISSGGVAGAKLTPSRPSFVCTNCRRRKVKCDRKTPCTRCVKSGLVQTCTYTVNLDDLPQPEGLEELLEADGGMENLFGVSGLSVRSEQRLYPGQLDPSSTESQTTLDADANTKNEDGDSLVLRKEQSFLCNFQTLHYKRPLFVKNGDSIRYLSPISLDAIFSNNKLFEKFRLMMPCLFEEDAEETGKTQSTNEELRILSGSYTKTEIDALITQLILPNVDAIKERIAYFDQDKSVMLCMFIPVANLSRFMDVHFIKDPANEKGYLYQSPAVGPNVGVAYAVLANIFCIVQLVSIVTEMDETANFQYTMTISVDLIGHTALKCMCFADYRRKPTFELLLAFLLYRINFYVLDSVSATSNASTFFNIALDIAFSLGIHIRCDQFDNHTEDDVRAAWNVLQFMDSITSLYMGEPLKIDYRYCIPRLYEYWEPIVLYLRNVVAAFNSTDPITLNEIIDLANFAAKLLSIFKPFDELLNDTTYGPVQYLFSLVIKADFVICYQLLLFILRLSVDEIDELPIQLNDSDSALVEELKAKSECHLFYCLVVTFSLMKKISNGKISYHKQAVKLMVAMRTLFSRFMLVGNKLIFCYLSVFNTKPKKMGFEPPSTEIFTKNDSLKFSDISLYDAEKYLCYGLDGMSTKDPEFQKMKGLMKSLLSINEFLTDFYISMSDKSPLLSYGKFQSHFKFLIFICKLLRNVYDFESFWSTRDDEARFKIDYWKHIIDKTVTYIKAINEVGIDQVNLSNKWDLMGPEWDFLKSDEVRDSLGYTDDESKEDGLEELYQIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNW1</accession>
    <name>A0A1E3NNW1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_119196</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018903.1">
        <property type="nucleotide sequence ID" value="XM_019159858.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NNW1"/>
    <dbReference type="GeneID" id="30176545"/>
    <dbReference type="OrthoDB" id="4066896at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057454">
        <property type="entry name" value="Bud3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021895">
        <property type="entry name" value="Bud3_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12015">
        <property type="entry name" value="Bud3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25351">
        <property type="entry name" value="PH_BUD3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bud3 N-terminal" evidence="2">
        <location>
            <begin position="66"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Bud3 C-terminal PH" evidence="3">
        <location>
            <begin position="483"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="945"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1096"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1711" mass="193375" checksum="079D06901E721BF2" modified="2017-01-18" version="1">MCSTGMPTKEDGSSPTTTPITRIKSIIPNSKSKNNLFMTFFDNTDPIVRSSSQYIITQTKEDLYNENYWSLMIPSAAYFHVYNSVLGDVFAMVYNNNIRNEKNHSLSTFIISKHGCSVHPEIYLSADSKYYPAVENLNPSLKSSKVRKALAISLLKTFADLNSDITDSLYSQADLPNKTFDWDETQAGLLASQMTLLDALPPSDIGSYLYELGYLTQSLSTASYVVDVIYTDQPGNELLSEQNNALAFLLGKQLEQLFDPLTEYSPEPTEKVYKPPIDNFSSCMEEDNDLICSICSELITVQTNYTVSLVQFLQNFIVPLRIKVLEGKLPGYSTAKLNQIFPPTIDEVTRINCIFLDMLKLARPYGSYEILKACGTTIPYFYKAQMRHEAATKNFHLNYLKFVADVNTSDCSDLLSLDQPTVETAVYSSLNLVKIQLIIQRLVKNQTWPDQLKDSVNIYLDSCDDTISSFANDKLLPYNGRIFTPTGKILAEIAKGWPSELQFGWLTRRVVAVFDATDILANNVRNKSVIIVFSDHALFLTIDDDEYYADLWRGNESQDTNNGKNKIANTELSASMSNSDYVSSSIHKPSVSDILMHSLTNETPLNKLPHMSVKYWASINDLHALHFVSSAQSADNTPNSYVRFFNEKDSSFTGIYQLDKVSGKYVTEVLARSKILNKTQSFHLFCGSISNDDLKPTENSDDSENQNGTITKRVYYTAHEASTYEKEDTKSPFIVLFNKEYNDDILDEYNVYAFITLNFVKDDTIRMEGISRCRIDGHEKEKLSYDVNVDILSASLSLILSELFSTHMSLYNPMLIDYLLTNNGQVNSQASKVLNVPLEVLDSDKTKIIDSVMKSKKERDIVNSVANDKKDRRKSLELLSEDIKTKKLTKALQKKEVKENKEVRFQAAPTMKDQSKTSNNPPRNLEKSSKGGKAKSGFFKFFSRQKPSNKTPAANKQLKRNATVVYQKKESTQKNYDYSELKPAKPKIQKKKSSQSFTLFSLKSNNLSVAKREAKVQPVKKEVSKPIIPKPNNFESSIANPISPQKIVDNNATSVLEKEDITEMGHRLTNSQTDNTTLSTGIYVNSHFEFPMESAPASQNVDNDKSSAPVTPKIAKLKDGPGHTKGYKDSVDLMGKLNIQIEDPKVYDDDLFELSSNNVSKTLQKSHEQFNIMKPKSIHSETTQTDDTNVFTPKAYDFTPVTQISSRNPAEVKDSDYVDEKFYNNNMAIKTDQQNKNNIIDANVKRNPWRVMTLPVKSPEVDPDMISLQRSKSFYTRFKNMRERQEQMLKENGMTLVRDLSDLPIKDRKALSQGFIFSPSICSNLDKSILDNENTNWTSMDNIMQSTSTETDLPAIAKLAPQKPPNLTTKPLISSPSTSISVVERKIVQTKPIVKESPENSRSISTSSSVYLTSEDDALKTYDNLQSALNAINPYSMVIDDTNQPDIYEDELKEVSSYHVIDDNDDATILHTPVYHSESVSNVKLYQDDIDFFSDLDLDKMDLNIDMNFPDVSELLINSVNIKQKPMHKSKSFVTNMESNETLSPDFTIRDLTIGNLDDTIMHSQIDTSFDSAQTNNFNSNMLISDRRDGTYNKKDGAQKSGFSRSDTAFALNALLKNKSYAYLQDVFNGDYESCDDIGSKPETGLMTQIFDEEFSEFEDSTSDSVLRSKKQTKSSRLKREMTDYDIVYYQKLLNSSVNYLSSYTNDEEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP41</accession>
    <name>A0A1E3NP41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cleavage/polyadenylation specificity factor A subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_120117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018955.1">
        <property type="nucleotide sequence ID" value="XM_019159865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP41"/>
    <dbReference type="STRING" id="763406.A0A1E3NP41"/>
    <dbReference type="GeneID" id="30176552"/>
    <dbReference type="OrthoDB" id="3992188at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1239" mass="141432" checksum="6EF74E0AA7551C62" modified="2017-01-18" version="1">MKKLVYIRTGRRIWRVTMSLNQQSESNACYIQTLLHSRLPTHIINNFKINILRNEDGSVNNNRYTKLQKSSSRTLEFRVASEDWKSLLGEKVLEQQAAKLPLSETQIKSKDELDDLDSDVKMTNETYDDTFKPFLDQESKFVTIMTYHSSFSIDNGPCIALPSQIISAGVLRENEKSQEDTLILHLRNSLILIRFAFGKNDQFVPFVVYSSQSISNLGGSKICVNSQKTLLILSFFVRDFFLHTINYDSNGVPSLHHDFNYSFQYSILDQCIVPITESDDAIITISMQGGILCLTSICNLTMPTNHEIMLKNTFPTPLYAVGLAETGGVLLLQENQYTIVSLSFCFTGDESGNYPFNYPETSGGNFKVNSFYIPTRKIITSFSSDGFSEENYKHDQVLISTTRNKSVYLLDVYYDKKRHHFTSTIHRLFKYKHVISLFVFEPVDDSTFRFTFANEMGITESKLVSIVTENETPLLKKVEDIWSQVNPYPMFDYELIASSGVRNTIENPSNDLWAITGCGLNHSLMNFKFGLIADKTQSAQKLYDIDQIYSFQVNHTDLIYVWASGEMRSGLLQLDNSFDNNGKFVSFIGENLVNEPFLCIDRTNQIEQLIVTPTNIFLFDTTLHKLSMHLKLPSECILASASNNIVALLHKDEKDDSIKFVLLKIIGNTEFRPLKMENLEFDPKLVSMMQFVDIGNKCYLIIGDFNGFCFLFEESGVDGFQFVYKEKIRMSSYESSQEQIWSPEYIFVPYKLHQLGSANNFILSSRNGDYSIVKFHISSGNYEMQNLHSVKLGDTGEVEILPTDDPHVAYLLCASLWKIDCRNSLFPEKIVIDGLKEHAIVACTVLPTLKPGQNDKLLVLRGHTLTILSIPSSFSVLTRSKRLETQCMKMNYFSNLDLFVLLPVPDVEDEKNGKLVFVESRTMRVLETGTDFTEIFKKDEIPLCLHTWKVHETSGTYTNLVVGCQTDKGKGVVKVLRLNKDDTKAYAKLVFSYKEDEPVFHIDSLGQNRCLLYSTGKKIIRWLYSADDTRLVERKIVIELQDPIKKFTLKRAENKNCVIKSFLLLAVTTNNKSAYAELDEILCDPVIKEEKTFTEFCSDGLISGDDTLITSNFNKESIIISSIHNELAIDRSTHHIGYIPRLTFINSFPPWVSIKDRCNYEEQNFLTIGLNGQIDLIRFANENNSIYFDLKESSDNQEQPQLLSTYTDQSLSQFSLVDTRTFENASDIKDDVFMSNILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP51</accession>
    <name>A0A1E3NP51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CREG-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_50902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018993.1">
        <property type="nucleotide sequence ID" value="XM_019163148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP51"/>
    <dbReference type="STRING" id="763406.A0A1E3NP51"/>
    <dbReference type="GeneID" id="30179835"/>
    <dbReference type="OrthoDB" id="10513232at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055343">
        <property type="entry name" value="CREG_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37273:SF1">
        <property type="entry name" value="ADL397C-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37273">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13883">
        <property type="entry name" value="CREG_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133445" description="CREG-like beta-barrel domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="CREG-like beta-barrel" evidence="2">
        <location>
            <begin position="56"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="247" mass="27556" checksum="2C1467524E3815B6" modified="2017-01-18" version="1">MLSLRVLSLCIGVWLLLSIVGECNAQQILRAEHCEGSEESNDNPFKRPKHVYRDSRTASRVARRLVGNNTVAMINTQFEHGNDTVAMSFPEYAIDCFDSGDPVLLFIKMSHNWRNIAVQAGYDSSVTYTVDSSEFDADWPGASPGSMYGMPRVNLRGHFRLLRVAGDDAFDPEADRIQQCFLSAHRETGSWFPGDGNRVHSSVWTEFVVDSGYFVGGFGGYAYIGALGNDEYHYQHSQDVDEGSLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP92</accession>
    <name>A0A1E3NP92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71914</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019012.1">
        <property type="nucleotide sequence ID" value="XM_019164047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP92"/>
    <dbReference type="STRING" id="763406.A0A1E3NP92"/>
    <dbReference type="GeneID" id="30180734"/>
    <dbReference type="OrthoDB" id="545683at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04938">
        <property type="entry name" value="TGS_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="5"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="418" mass="46098" checksum="246A12C572538CF4" modified="2017-01-18" version="1">MPRDPLIGIVGKPSSGKSTTLNSLTDANAKIGSFPFTTIEPNRATGYLKIDCACSRYGKEELCKPNYGWCENGSRHVPIDLLDVAGLIPGASQGLGLGNKFLDDLRQADALIHVVDVSGTTNAEGKATRGYDPLKDIEWLQDEIRLWIEGNLNKRWGSIVRRHTSTKSSIVETLRAQFGGYGANATLVREAIDSIENLPPLEEWDNEWITTVIKAFMRFKFPTVLALNKIDHPDADKNVSKILLKYPDTRAVLTSAITEIFLRKLKKQNYIQYEEGTEFVDTLEDLGPDCGLKPLDEKLTERLEDIKDLVLYRFGSTGVVQVLQEAANVLDLVPVFTVRNINTFTGNSGSNVFRDCMLFKRGTKVGSVARSIMGEVTIAAIEGVSGKRVSPDDTIDNNKNDILTFKVAPGGVSSEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPG6</accession>
    <name>A0A1E3NPG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_15862</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRD1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019124.1">
        <property type="nucleotide sequence ID" value="XM_019161000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPG6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPG6"/>
    <dbReference type="GeneID" id="30177687"/>
    <dbReference type="OrthoDB" id="7759664at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16479">
        <property type="entry name" value="RING-H2_synoviolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058051">
        <property type="entry name" value="Znf_RING_synoviolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF184">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SYNOVIOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="308"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="361"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="407"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="447"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="473"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ48011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="671" mass="76163" checksum="FCD3E7259C5D8CE4" modified="2017-01-18" version="1">MENWKRNLLFLGYVVVSIAAMVIKFYTLYDTSPNSFVLGVRFAKGDALLILTNMMIMLALVGGKLLQYIVFGELRLIEIEHLYERIWPTIIGLVVSASAYKAKENSFFRMSLFVGLLFSKVFHCIVIDRLDTLIQQYYQANGNSVTRLLFNRVVFTSWLFLRLDIPLLRASIDEAYLHKSPMLLGIAFELFLLIMDLAYAAIKFALNVFELYYIRRFPDEEVWSYKVWIDSVAKLLLGVVRCIMVPALFRYFTLMDSFPLNLLNEIFRSFYGLSKSVGSLYRLIKNAMKLNNSLGYPTAEELQDDEICIICRDDMVFGGTGTARSVPRKLPCGHILHDGCIRSWLEMSNACPTCRKDVIRNDSANTGTANNENGNENENENENENENGNNVAAVVAAPPQNLVEGLNNMIINGDDNNINNDDEDGNSNDTGESGDAVEVERLTKSANNETNENNTDDQNVNDNRTGNSKEEIANNEETEDEGIDEENGDVEIAFFGTNNMSIPRPTPTTRSTQLLGIYPRYMPSFGRSDYFEVCSSEEKEPRSTAHLHSERMNQIGNVVDKIGSLNMDEHQRGRWKRVRDNFELARSGGNQGPELNAGDGSGEGVDESNTEFVHEVPPHTVIPKDWTIFPIQKTREEEYEVKLSRKRRVTMKVVRNGRIIDPNTFDKYTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPI3</accession>
    <name>A0A1E3NPI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019144.1">
        <property type="nucleotide sequence ID" value="XM_019161001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPI3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPI3"/>
    <dbReference type="GeneID" id="30177688"/>
    <dbReference type="OrthoDB" id="8883818at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="655"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="664"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="733" mass="82614" checksum="5575CC925E65C49D" modified="2017-01-18" version="1">MDIHRCSFLDYTPESITSCAFSHESNLNELTPKNFRLAVGRADGTIEIWNPHDSRSKWVLETIVPGSRGNSIEGLVWSSNNDPSLPPRLFSIGGSTILTEWDLSTGTPLKNYDCNAGVIWSVAINSTHDKIALGCDDGSVVVVSINGGPGVIEHECILQRQKFRVLSLCWIENKMIIGGCADGRIRCWSYNNEDIIQDNDQEADDNTNMNIRGRLFQTLRVDRLKGEPSLIWCLLYLPKSNQFVSGDSTGTVKVWDLKHLVLQQSFQVHEADVLCLAKDASGEKFFTAGVDRKIFNFNYTKMSKSNSKWINMTNRLLHGNDVRTMNCYQSKNLDFLVSGGIERIMHINTAVNFQSSVSIKLPINPLIENIIINEEKRLVLMWQKNEVKIWKLKLDHTKKLVAKLTLSDPENITNVDISSNGNYLLVSRLSLVKLFKLEEVSEDKIAVKRMDYELLSAIGAKQSKFINSEDLILIYTSDNELISFKFDQISPSSPFDDFQEPVDYDVPEGISTGASDFQYLIYHTHIAIDENNSLAALSKFDGTIDILNLKTKESFTLIKLSSVATCIAFTSNSTLVAVTLDHKVHEFNVLNNKKDGQVYTAWAMRNFNSIPAHFLSLPGCAFGVFENLGRICVYGCNWLCFFDLNYDLPSIKQVPTENKKRSRNGTQLQATNGTSAKEQQPSNNEENKCFWLTKNYTDLIYAGKLNESELVVVERHLEDMANPPAFKVNKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPN4</accession>
    <name>A0A1E3NPN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AVL9/DENND6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15903</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019172.1">
        <property type="nucleotide sequence ID" value="XM_019161014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPN4"/>
    <dbReference type="GeneID" id="30177701"/>
    <dbReference type="OrthoDB" id="26278at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018307">
        <property type="entry name" value="ABL9/DENND6_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051731">
        <property type="entry name" value="DENND11/AVL9_GEFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017:SF1">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09794">
        <property type="entry name" value="Avl9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AVL9/DENND6" evidence="2">
        <location>
            <begin position="311"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="806"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="988"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1077"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="830"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1077"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1143" mass="127800" checksum="711B2DA4F8008524" modified="2017-01-18" version="1">MARKKSSKGKAKKDAMPKTGAENSIERKNVDELDMNWTPAELDKAEDRSDNEAPEDVDGEADENGEKEEEEKKEEEKETDGRGDGNGDEETLSSEAQAPVPGEIAQDRGEEVEDTRESDKEDVGAEEEDKKQREEKAEGDGEGDSEGEGEEEKDEEEHNDSTANRFVIVDSEDEEEFEKEDILQNEKIKDDVIEKSTVKPESDSLLNKPTEPEAKSASEELPETPSIVITPQEEPRETPLVTTEDTKMEKESKVEIGQQPYDPEGGQIASHESRSSAEPRDLAFITPTPLYRYIENKETRDKMKSLTSNFVFGLAVVDFDHIKGPELTYWLDDEVISAQDEGQSDKSIQGLLQQKIRHYSKIWPYLAFQALPDGVHMYGETFTQFTLCYDELKKTDVELDFDLVDLVFDDEAPKAKTGIGAKVEQSDVGAGADAEAEADADVSVKTETDANGNFVNVDFTKTEQATGDETSLEKSRTRPVVEMEDPNQGVITLFGCACIRQLDTALLKNIDKTHLKRSVVQKSVILLTRSPLPIQLREKLSIITQSWFEQRDFSDREILKALYVHVSSTYNQNGYLIEDDDLYEVNNNNNSNSTVEDSCKIIKESDFYMGLNFQEVVRKLRRQLLVIFKCLVLGKSRILFFSKDLNALSNTQYCLIGLISNLLLNLSDSGYPLSDNFLHEEKMTSQSLKSSDRASILRFLGLPLRIFGFGSFFQPYLTLQQLSYITNINTKSFVVGSSNDIILEHKKDWFDVIIHLDEKESGLFGSGGCKVEILNKSLKDKVGLTWDDKKFIDYIIHSVDTHYLQDAKERPEEQEGEEKEKADEEKHDAGSATTATTSSAAGSGEHRSTREQIKSKTVNSNLSIDNGVYKGGDDFIRSQFEDYLIGFLSCVKYHDFLRRQATREKEKEVVEQLRLDTFDNDIARFGARWVEEFRRSQVYEHWNEITENELFNFFEPRHVGKDIGKDDAAEYQFKGGDVFKSWISRWKEAGRSPAPSPGPGSGAGDAETGKVAHADSADRGDKRDKGDKGGAGEGADKAHEGRGADPAAAAAATVKTTMNTFGRDVGLFFRKIGDNTSQRLYSSNPSTDKDAPAEEHKQPEDVPARAKPCAEADAAERGNAADTEGAPGSGLESRLRGLFQWGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ22</accession>
    <name>A0A1E3NQ22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16025</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL49 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019327.1">
        <property type="nucleotide sequence ID" value="XM_019161061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ22"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ22"/>
    <dbReference type="GeneID" id="30177748"/>
    <dbReference type="OrthoDB" id="19439at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007740">
        <property type="entry name" value="Ribosomal_mL49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05046">
        <property type="entry name" value="Img2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="117" mass="12759" checksum="78A50DC60200F669" modified="2017-01-18" version="1">MSAAASVSAAAAARLARALPALDSISAAQLTIKPKLASYHVPRTHLGNLPVYKTYRSQAVYTDIKRVQGNVVQLRNDLQQLLPHVDRSNFNCHTISGSLRIKGDHTLAVKELLAQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ70</accession>
    <name>A0A1E3NQ70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019344.1">
        <property type="nucleotide sequence ID" value="XM_019161068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ70"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ70"/>
    <dbReference type="GeneID" id="30177755"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15550">
        <property type="entry name" value="PHD_MLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053051">
        <property type="entry name" value="HDAC_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="5">
        <location>
            <begin position="87"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="211"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="339"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="546" mass="62677" checksum="1EFEAD192972D912" modified="2017-01-18" version="1">MSSRRSRRAHSTQPKYATEDFEMDTDFDSYNNNNDITGSASKRSSRARTDEPDSHRRDSRSQDPEQEQEQKPDQDLDDINEVEEVTRCICGSDDLYVPKNSHEEFDNVDPGFFIQCEICAVWQHGYCVGIKDEENAPEKYWCEQCKPENHTLFTDRFGIRRSKYDPTHENAGNSSYSSSASMNSQKKSLKRKNGRFVSTKAENPESADDLNEVKKERKPSEVKAIENEESTENHNNDHEMSQNEEEYEDDDGHRRKHRKRSSYSYRDYNYEEMLKKALEESAKESGVVPEEVNISSSEGPGGVRETRTTSLRKNRAKLHDDISLTAPENEDSNPETESELPKVDDGSTKTDTELTEVKPEKVEIYKKPKIEERRRARDPKRTRPRRTTSATSASNDEHEEDNDDRSDAKSVSTKASSSSFNSKNTTNPNTNSISSTPKRSSAKREKKDSKPPATDDKPFRANIPSARISMNEMTRRIFSIMDFVSNIQINLSNEEEFKNNLFKMNDSELTQEITELKSTLIGCYNDSIDQLDQLTNLLNTWQNNHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQD9</accession>
    <name>A0A1E3NQD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_125750</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019398.1">
        <property type="nucleotide sequence ID" value="XM_019159947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQD9"/>
    <dbReference type="STRING" id="763406.A0A1E3NQD9"/>
    <dbReference type="GeneID" id="30176634"/>
    <dbReference type="OrthoDB" id="5377392at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="11">
        <location>
            <begin position="73"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="12">
        <location>
            <begin position="189"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="348" mass="39508" checksum="84DBD329662BDF61" modified="2017-01-18" version="1">MSERVIVLNGDNNCSLKPDSGKIKQYLSTVLSLIENGLKNGDRITLQIHSVTKKFTSTTSKFSFPSLKPEVNHKFAVYFRVLRLIHNQISLNESVSKRQIYYMDVSLFKKQSVVDQCIDNLANSIGVSIEHLNVHASQKGLVYANLAFDSFKASRESGCSLIPYVKTSAQKNDIKVLFNNKIGETPRSIIILEKDAILSALVNKEKESTSKQFAESILVTGRGFPDRQTKQFVSLICQTFYNTPVFGYFDADIYGFMIAMEYKNKPMDQVRGPCCPTMQIKGGKLSPHIKGENERLDMLPITDRDINLTLSQLSNFGNNKLEKQFTEMQRSLFLCVKRELRIEDINCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQJ0</accession>
    <name>A0A1E3NQJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_81379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019492.1">
        <property type="nucleotide sequence ID" value="XM_019164986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQJ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQJ0"/>
    <dbReference type="GeneID" id="30181673"/>
    <dbReference type="OrthoDB" id="341300at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032048">
        <property type="term" value="P:cardiolipin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120010">
        <property type="term" value="P:intermembrane phospholipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001247">
        <property type="term" value="P:positive regulation of phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="183" mass="21529" checksum="4E786AE4C7263F58" modified="2017-01-18" version="1">MVLYYRNNHAYEYNFETVTLAYFNRYPNPYATHVKSIDTIDVYMDDSGRLHQVKLIKKSGRLPQFVKPFLGKITTTWIVENTVVDPKSQEMTTYNCNLDHRKIIRIEEFNTYKYSFAQSVTNSFVTVKFSSGFTHRFGLGLGIKDRIENWSKNKFSDNLMRSREGLRLVMDSVKLKLLARNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQU4</accession>
    <name>A0A1E3NQU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_70103</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SDA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019583.1">
        <property type="nucleotide sequence ID" value="XM_019163551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQU4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQU4"/>
    <dbReference type="GeneID" id="30180238"/>
    <dbReference type="OrthoDB" id="2196187at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048292">
        <property type="entry name" value="SDA1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007949">
        <property type="entry name" value="SDA1_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21638">
        <property type="entry name" value="SDA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05285">
        <property type="entry name" value="SDA1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="9">
        <location>
            <begin position="64"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 middle" evidence="8">
        <location>
            <begin position="542"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 C-terminal" evidence="10">
        <location>
            <begin position="710"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="517"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="684"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="569"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="758" mass="86357" checksum="23362FF6D0C3037E" modified="2017-01-18" version="1">MAKRNRAAILPTNFALLQNLVRRDSESYYEEFLQQYSHYESMRDIFLLNPSTEDSEEFAEIINFMSFVCASYPKETAKFPDELATILTKNHAELDPELREKIVQCLVMLRNKDIITAEYLIKTLFPLLFAYSSNTTNGSGFHSKALRNQIYTALISLLKSCNTGAKNQKLNRTTQALLFNLLDQKDGNGVWATKLTRELWRRGIWDDSRTVELMTQACLHTDPKVVISGVRFFLGADKEREQAFEEESDEDEIDPSALRHKMQVNKKSSKRGKKLESVLKTVKKKNSNKHSATYLNFSAIHLLRDPQQFAEVMFENNLSGKNANKYDLDQKIAIMSLVSRLVGTHKLTVLGLYSFFLKYLTPKQRDVTQILAAAAQATHDLVPPESINMMVRKIADEFVSDGVAAEVASAGLNTIREILARNPAGIDEDLLQDLTAYKGSKSKAVLMAARSLISLFREVAPEMLLKADRGRAATMGLIHNGPKNLPQFGIEKDTTNGIQGLELLAKWKMEQGLLGKEGENDWDEKPFEENEEQDIDDVEGDWVDVKSDTEINLSGDEDNGELPKERNGIKKKYLKYMRQHKKRKASKNDEDDSDLEMSDDEGNSGKGRKKTKKQLELEERKLKEQEAIRETMNSVLTPADFAKLKELNAEAGIESVTGHKLRNEDEVDSSDLVNVDRRKMNKEERIAGIMEGREGREKFGSRKGRRDAPHSTTNREKERKKNFVMMVHKRAVQGKKKMSLRDRQKVLRAHIERQKMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQU5</accession>
    <name>A0A1E3NQU5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein IFH1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019056.1">
        <property type="nucleotide sequence ID" value="XM_019160975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQU5"/>
    <dbReference type="STRING" id="763406.A0A1E3NQU5"/>
    <dbReference type="GeneID" id="30177662"/>
    <dbReference type="OrthoDB" id="4047468at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018837">
        <property type="entry name" value="TF_CRF1/IFH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28057">
        <property type="entry name" value="PROTEIN IFH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28057:SF1">
        <property type="entry name" value="PROTEIN IFH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10380">
        <property type="entry name" value="CRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="683"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="856" mass="95504" checksum="9F4F9016E61C292F" modified="2017-01-18" version="1">MTESPRKIPLKPEKSAKTIAKKGPYATKKSSSRRFSIMSNSGSDSDSESDSESESSYDDHIYLNKNKSFASAKQSSNINVQASSGSDEEYSSSSDDENVDFVQLTKQRRLKAMKAVKSLKQQINETTSSISPSPSQSPMPDQLLEQEEPELDDDLVDAVIESEERKMKGVDDDNSNANAISSVADYSDAELNGDFESMNFDFSFEDTNDDTANDVKGKHSVPPAKDSKNLLDRLRDEDIGEVVVEDTIKASNSGSQAVLNKVDEKKLEIDVPKFNEKEINSDADYEFDNDDLIQTLLKDNDDLELQNLDNSDDDFDYLIANQHRIDQNAVNKSASKDLSLDSINTDDAYLMKEETQAMLDDFNKTAKSQASLKNRRSSMYYQTQPLNITNDEELDDLDLDLLNNFEQFLPKQQAMRRRSSILNQTLLSSSSDGEDINMSTNINTIKRNKKLAKKKAKKGRKLTNTESSAHTKDIRKMLGISNADSNFTSNYTSNAIVDSDDDTKLLEYIFEDSESDNISASTDNVVDNGNETDEDVNLPKKSGKILGSKHAKEILSSSKDQFRAPKLGTFITKKPYSVIDGFTTRFLQPLVGNHSVMQKAAAINKPDTGESSGIVNSNNLALDELINISEFDEDYSNVELKEDFDDFFGDKRIPLTAFRNKGLINNNGNTSTNELNASARRFSFTDRNQRRKHHKSAKSTTKSASKLVKPSNKPYKIKKASFHQPQPLVGALVPKSPTSIDTIDSSIGKIHDPASVDEMLLIGVDSIPFTEDYLDPHHSNFEITAPHQFTSQRKRKSMSRSSKARIRRASIVEAHAEGLRQTKNGLFDETIMDDVEALLIDMGDAEEYGFLFGNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR59</accession>
    <name>A0A1E3NR59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_9101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019679.1">
        <property type="nucleotide sequence ID" value="XM_019165067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR59"/>
    <dbReference type="GeneID" id="30181754"/>
    <dbReference type="OrthoDB" id="3997252at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="189" mass="21482" checksum="B4383DC9659D8EF0" modified="2017-01-18" version="1">MSEALQAIANTNASIAGTDFKNSGIFTNSLLKTTDTTQIIRDIRDEEVVFFQGKKMVDIDYELINNSGFSTSDGFAQHEFYKLLEDEISTASSLNEKFDALLPPEFRDSHEYATDIDNMRSIYKRTHIIAKIWASPTGNMLDHETNRMLENVKAQLDALLQTLQEVSECQALLKKQKNELNQSYESKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRM8</accession>
    <name>A0A1E3NRM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YjgF-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_75421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019890.1">
        <property type="nucleotide sequence ID" value="XM_019164937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRM8"/>
    <dbReference type="GeneID" id="30181624"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13708" checksum="8666B97E962D0F84" modified="2017-01-18" version="1">MPATKVTWESIQGQEHPVLSPAFVSNGHVFTSGIIGSNYATGETPEDLEEQVHLAIKNLEKVLVAGGSSLDKVFKVTMFISHANYSSTVNKIYGQYFPQKPARSCVVVAFMDAAIKYELEAVATLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSK8</accession>
    <name>A0A1E3NSK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_70278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019771.1">
        <property type="nucleotide sequence ID" value="XM_019163600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSK8"/>
    <dbReference type="GeneID" id="30180287"/>
    <dbReference type="OrthoDB" id="3979469at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="146" mass="16842" checksum="026B0F41647B65D1" modified="2017-01-18" version="1">MAADNEHSEAADDAKKLASYNDTVFGGSFSNQNMHHTSREHMSRLQSVILKSTVVGGLYGALISIPSELFIRWRFPLYRAFGFRIRVFYHTIWIASAAAFHTEREVIRFENIIRQEEEEKRRQLLELSILQGVYTPESEGYKSKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSQ0</accession>
    <name>A0A1E3NSQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Defect at low temperature protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DLT1</name>
        <name evidence="8" type="ORF">PICMEDRAFT_70315</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Required for growth under high-pressure and low-temperature conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DLT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019811.1">
        <property type="nucleotide sequence ID" value="XM_019163611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSQ0"/>
    <dbReference type="GeneID" id="30180298"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038869">
        <property type="entry name" value="DLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021:SF1">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="232" mass="26855" checksum="87EE94122A18ED8E" modified="2017-01-18" version="1">MVGRYLKNTTHSGLLWLYTSSFVVIMIIILSMSSVLPIDVIVQSKTNNSHLATNTVIILVICVVFLFISAILHMFRLFYDNMLLQEIPKPYVPITPNDVGKSTSRTIEREIVRCKEILERAKPRGDISHPGLFHQSEYNHDVELPDNLIYENVVNVIGQELKYNGTLTVGDDKVLRLDNHYTLRELLHVYEDDEMVGKFLNLYEKLRFSGEPITCDEFKDFLQKWSYVKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT14</accession>
    <name>A0A1E3NT14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_29742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020325.1">
        <property type="nucleotide sequence ID" value="XM_019162606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT14"/>
    <dbReference type="STRING" id="763406.A0A1E3NT14"/>
    <dbReference type="GeneID" id="30179293"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF21">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="256"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="61596" checksum="A20892F42D46E172" modified="2017-01-18" version="1">MSESILAPSRRITTLHQHVPKHPLGVKPLGNQIFASSGDLTKIRDSAGKLFNALPDDIVLSVFSLLSKDDLANASRVSKFWYAYATFDELWRKMYTSVSSETRQELGIEFLQWKSTWRSSILNSKEKKPLVDCGGLVYSDTLFTPYSNSCIDYKEIFRDLIREQENLRDIDESTFTYENFVNNHWTDHPFILGSKQNDSNKRWPEWTTKYLLERFPDVKFRQESVLWELALYESYALQNQDESPLYLFDCRSDAMKALLPTGYYEDPPIFAKYDLFKVFEECRPDHSWLIAGPARSGSTFHKDPNSTDAWNVVLEGSKLWIMLPPEMKPPGVFVSDDESEVMSPEGLAEWVKSGFWNDRFRTCVVGITFKNECMYVPSGWWHSVINLEDSVAFTANFVPPCKIGQVLDFMKNKPAQVSGFRHDLFQKKLRSFMEAQEIDDSENKTTLEKYLSMEELRNIDEDVGELKGTGCMPVYEAFVELLLAKGMDDIVAKGLRDLKTADSKQERSRVWKSLTKDAKSDEKGGFSFGFDFDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT20</accession>
    <name>A0A1E3NT20_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_70721</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020269.1">
        <property type="nucleotide sequence ID" value="XM_019163721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT20"/>
    <dbReference type="STRING" id="763406.A0A1E3NT20"/>
    <dbReference type="GeneID" id="30180408"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03388">
        <property type="entry name" value="PAP2_SPPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="10">
        <location>
            <begin position="126"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="429" mass="48117" checksum="C5017B1746EBF9A2" modified="2017-01-18" version="1">MPKPMYMPAAARNGSTATGNGTGNGYGHGHTDDKPLNSEALTDTHLHDVGLHPESYYDSRLPFYAAFMRRQLLPLVKLELPLLEKIQQFHSHWLTMYFLLTANVGSHTFYVIMLPMGVWLGSLNFTRDLVLVLGLGIYLTGAVKDLLCLPRPHSPPLERLTMSHYTAKEYGCPSSHSANATSVCIILFLHIYNNDFFSAPVKQSLYVSLVVYWITLVVGRLYCGMHGLVDVVTGALVGIATVLLRMATKNFWDYTLLSNNDTLLTKISPLLLVGFYYSLIYFHPTPVEPCPCFEDSIAFIAVLLGKDLADNSMRPIESAESLSLTLMDIYQTHPAKHDYEWARSGIILTLMRVIVGVTLVMIWKAIAKPLFTFLIRKLRNEPTVKTTTPEPPFAFISRTDTKIFVKFIVYAGIPTVAVYSMYAFRFLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT52</accession>
    <name>A0A1E3NT52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Activator of Hsp90 ATPase AHSA1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020353.1">
        <property type="nucleotide sequence ID" value="XM_019160491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT52"/>
    <dbReference type="STRING" id="763406.A0A1E3NT52"/>
    <dbReference type="GeneID" id="30177178"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF15">
        <property type="entry name" value="HSP90 CO-CHAPERONE HCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="150" mass="17396" checksum="F7ECD3C991FD2042" modified="2017-01-18" version="1">MATVHNPNNWHWVEKNCVPWAKEYFDENLYKVSVPSGEYKISVKSINSVSGDCDVTQRKGKVKCLFELKVEFVVIVINSEQEEEEVTIVLPEFEHDYDESDFNFEVRTSKLPCKTAVRKQFLPLAVKEVFLKFQPDLIATHEVGMKHNTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTG0</accession>
    <name>A0A1E3NTG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alanine--glyoxylate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70899</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.44" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020463.1">
        <property type="nucleotide sequence ID" value="XM_019163767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTG0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTG0"/>
    <dbReference type="GeneID" id="30180454"/>
    <dbReference type="OrthoDB" id="7403325at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000049">
        <property type="entry name" value="Alanine-glyoxylate aminotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000027">
        <property type="entry name" value="Serine--pyruvate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="11">
        <location>
            <begin position="59"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="386" mass="41518" checksum="BD0A3FE740C702BA" modified="2017-01-18" version="1">MSARKLTFIPGPIEFSDNVLAAMSTPSQAHTSPEFVKVFQSVLVKTRKMFCSAAASKSQPFVISGSGTLGWDLCGSNLVNHSDKVLVLSTGFFSDQFANCLKIYSDSVDVLEAPEFGASVDFKQLESKLAATAYDVITITQTDTSSGVLTDVEAVSKVVKKISPKTLIVVDAVCATACETLKFDDWGIDYVLTASQKAVGVPSGLSISIASSRAMEKALQRPKPSSFFADMKRWAPIMQAYESGKGAYFATPAVQLIHALNVSLDELLAADTQTIDARVAAHKAASDAFKDKLVNELGLKLVPVSRDVAAHGLSVVYYPENVEGAALLSKMAANGFTIASGIYKEYKDKYFRIGHMGVSAVGERKQELNECFEALKLSLSELGYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTU1</accession>
    <name>A0A1E3NTU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_9970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020617.1">
        <property type="nucleotide sequence ID" value="XM_019165155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTU1"/>
    <dbReference type="GeneID" id="30181842"/>
    <dbReference type="OrthoDB" id="3997159at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38129" checksum="0D5E3143FD276528" modified="2017-01-18" version="1">MFPRATTARLLTNACRKTTPSLQRSALRACCYSTASSSQSTAGAHSADSARGTSPPPEFEDYPYRYGYGGWGRGWGRGWGYGYPGPHRHRRGRFFFFLISFAGGMFAYKLLSGNCGDERWERRHARMHDSGSYPQDAYPGFSSAAASKLDVQTLPIVAALRSNPDFEEAHLLSSQVVTPTTNSSFEDSIMLPSKPVEFASSSTGERVAVVTLPEIAGRHREWYGFGRRSGHRRGGQSDRLVMLMDEYLKRDAALLLNVPPSSIYTTALDVDFRSRMTPHDKIIIVKATVDENAAKDGRSVAISGSIMSTDGQLISKLHGSFTADSTKAPPTGQDVYHFRKWMWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU33</accession>
    <name>A0A1E3NU33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">General transcription factor TFIIB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14984</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020662.1">
        <property type="nucleotide sequence ID" value="XM_019160622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU33"/>
    <dbReference type="STRING" id="763406.A0A1E3NU33"/>
    <dbReference type="GeneID" id="30177309"/>
    <dbReference type="OrthoDB" id="25790at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001139">
        <property type="term" value="F:RNA polymerase II complex recruiting activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001113">
        <property type="term" value="P:transcription open complex formation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20551">
        <property type="entry name" value="CYCLIN_TFIIB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="10">
        <location>
            <begin position="14"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIB-type" evidence="11">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="339" mass="37437" checksum="7B7EA692372B2FEC" modified="2017-01-18" version="1">MTTAERRFPRGVNLNVTLSCPECKVFPPDLIERHSEGDIVCGLCGLVLCQRIIDNRSEWRTFNNDDQKGDDPSRVGEATNSILDGDQLNTVISAGIDASRAARDLNRTQSRSFADKKSHILQAAFAKITQMCDGYQLPRVVQDGSKEVYKLVYDDPSLKGKSQESIMAGAIFLGCRKASVPRSFKEIWALTNVPRKEIGRVFKAINSIIQRKNEEQGGLITYQNDSINTRHTNPEDLVGRFCSHLGLSPQVASAAQSIAKSLKDVGVLEGRSPTTIAATVIYFTTLVFDQKITLSKISEKTGVSEGTIKSSYKLMHSARQKLVDPAWIESGRVDISKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU80</accession>
    <name>A0A1E3NU80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_95722</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020236.1">
        <property type="nucleotide sequence ID" value="XM_019165104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU80"/>
    <dbReference type="GeneID" id="30181791"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="171" mass="18991" checksum="B166F841F42B1258" modified="2017-01-18" version="1">MEYSSKFLPFTVRNVLSHSSLDKVCSLRPFRLIFPRLLSPLFCSFLSKKYLVSTGQPYFPTPIPDQHVCVTAIGSCSMHAAGTSQRALAAKRKTARTEQDRYHLSQLLCNGVQQILHQLLLVKKVPPFASPYENPCQLSPIPRGIANSQQFLAAGVLGRQGSRSTSSFPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUF0</accession>
    <name>A0A1E3NUF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14737</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020381.1">
        <property type="nucleotide sequence ID" value="XM_019160502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NUF0"/>
    <dbReference type="GeneID" id="30177189"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000001">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF34">
        <property type="entry name" value="NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="9">
        <location>
            <begin position="104"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="8">
        <location>
            <begin position="295"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="66678" checksum="A7F346DFF6EE65DF" modified="2017-01-18" version="1">MTRDNCQVVKKTPVGEEARVQISHSTRLSVAGPIECDLAGSHLLNSPLFNKGSAFSKEERIAFGLEGLLPPVVNTLDEQVDRAYKQLHFLKTLLAKNDFCTSMRLQNKVLYYKLVREHIKELIPIVYTPTEGDAIIAYSDRFRKPEGCFLDITEPHNIDKRLAQFGEDKDVDYIVITDSEGILGIGDQGVGGVRIAIAKAALMTLCGGLHPGRVIACVLDVGTNNKKLLEDDLYMGNRFPRVRGKEYDDFTNKCIMAMQKRFPSAVIHYEDFGVTTARAVLDRFRHVLPCFNDDIQGTGAVVMASMAAALKLTDRNLLDSRVLIYGAGSAGLGIADQIVNHMIIHGATQEQARARIYCMDRFGLLLTGMKTNSPAQEGYAHNPKDWEGVDTKSLVEVIGKVKPTCLIGCSTQAGAFNEQVIKEMYKFNPRPMIFPLSNPTRLHECVPEDALKWTDYNALVATGSPFPPVEGHVISENNNCFSFPGIGLGAVLARATTISDAMISAAVDELASLSPAQNDPKAGLLPPIEEIDETSARVATAVILQCVKEGHSRVESEDSPVGGKVKVPRDFYACLSWVKEQMWRPEYRPMIKVEHSDEIHTHQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUK9</accession>
    <name>A0A1E3NUK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_28827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020376.1">
        <property type="nucleotide sequence ID" value="XM_019162556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUK9"/>
    <dbReference type="STRING" id="763406.A0A1E3NUK9"/>
    <dbReference type="GeneID" id="30179243"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000001">
        <property type="entry name" value="60S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15258" checksum="49B1F75672C1A492" modified="2017-01-18" version="1">MAKFIKSGKVAVVVRGRNAGKKVVIVKQFDEGTKANPFPHAIVAGVERTPLKVTKGMGAKKLAKKTKVKPFVKLVNYNHLMPTRYNFDVEPFKAVVSAESLAEPSQREEAKKAIKKVFEERHQAGKNNWFFTKLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUL9</accession>
    <name>A0A1E3NUL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14743</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020386.1">
        <property type="nucleotide sequence ID" value="XM_019160507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUL9"/>
    <dbReference type="STRING" id="763406.A0A1E3NUL9"/>
    <dbReference type="GeneID" id="30177194"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="BHLH" evidence="3">
        <location>
            <begin position="73"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="940"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="957"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1105" mass="122540" checksum="D482D10262AC12A2" modified="2017-01-18" version="1" fragment="single">MDAMMNKSRKSSSSLSDVSPNTMYSCRDESVSSNTSDSVRTHTSPVSPSLQSVLPSSKQAAAAAATADVTSRKSRSSHNKIEKKYRTNINTKFIELKDAVPTLRVLDNVTDINFDQLEGLAPASRLNKANILSKATEYIKHLELKNDLLLNEVRTLRANPNYSYQPPNSLHNVPIEVISNLPNQPQMSRIVLPSEFNNGSSNGSSMVDNNHMSSAYPADNAGVLSDSYRHEQSHTLSNRLPNRAAASSLHHQTSKQPILGSYPSHDTTHYIQSYNHVNEQSQAMCSSVYREGSIDRRNCYGYRSSMNNIKSEVLDSPSHTQQAAIQNHPPTEFTSAYTTQQPGLSVDNNSLQMQDMREHYYSQDGYNNSSSNHSNNNSNNSSIANNNMRHNNQLQEPFYEAPNARLARKIMIAGVTTLVGSNIVGSGASGGPAYGNAGGPDYHSMSASPIFFLLTQSIKLIQLVTFFSCVYFFVQPVVSGFINWKKDNAKKSDKYRSFYLLTFVGIIRFFLPGMSSSTSQFVSPTATNQAPEVFPIDLTSFPTSFCGLLFAYIKILNVSDCSNLEALKTKHPNLANPIEHTFNKLILLDLILRRSPFIGYLLGFKSRIEFLVNSLLRITQSGKTRDIHSRITDFIHCDPGFINSRSVVDQLARILASLHQTKSSALTAKQLYGNDSDNSGRGYNAVYEYLVNTPTNKLNLFELVAVLWCVDNVRARMVKFLGDVVNEGAKVDETIEGDIKKLIADIRKIETFVPTPCIKLIKCCKIFKSLLDPENESYLNDALIMILLSVEENLLLIKQNNDSNKAISEKLLAELHSPIPVLRKVLIVIHAAQISALSHTKNAASSSAAAAASSSSSNTASKFSQPIQLLSDENRLSLLCSIILHQYAIGNVDYGRSLIRFLKGERTRKFMCSDSVSLMASIATFRTIVVVLDNEKRNEGIANEDSEEEENGVKEENFEESNLEGEDEHIDDDNIVDDDLDDHDDDHDHDDELDFGHLSSDVEMLLTSTVSKRNGLETTIESDTDSEYDEGNNSLVCYSEGQSLNSNDHRILEDLLCGLRLYVGQGKTASAGDSTDYDILSLHYGLQSELSHRLLELAKELVGYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUU0</accession>
    <name>A0A1E3NUU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Urea active transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14881</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020531.1">
        <property type="nucleotide sequence ID" value="XM_019160579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUU0"/>
    <dbReference type="STRING" id="763406.A0A1E3NUU0"/>
    <dbReference type="GeneID" id="30177266"/>
    <dbReference type="OrthoDB" id="3991597at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="560"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="73249" checksum="935656255A1F773A" modified="2017-01-18" version="1">MADFQPILSQGYGYGFAVGIGAAFALLMAILTRILSRFMGQVQNSERFSTASRNVNSGLIASSTVSAWTWPATLLSSGAWSYTHGISGGFLYGVGGTVQITLFLFLAIQIKLKAPTAHTVSECFAIRFGTAGHWVYLCYCICTNVLISSLLLLGGSQGFAAATGMHVVAASFLLPLGVMAYTALGGLKATFISDWIHTVIIYIILIVTSYTVYCTSSLIGSPSKMYDLLKEVEEVFPSASGQSYLSFHDSEMLFLTWSVMLGGLSSVFGDPGYSQRAIASDAKSVFQGYLMGGLCWWVIPMSLGSSAGLACRALLLNPSFVTYPNPLSAAEIDSGMPVIYGMYMIFGTSGAAAGLLMLFMSVTSASSAELIAFSSVATYDVYRIYIKPDASGKELVRISHLAVFSFSMLMAVLSVVFNYIGVTVGWLLSFIGIILSPEVFAVTMMLFWPKMTKLSLLIGAPLGTLTGIGCWVGSTYVYGGHIVNKDTLMVSKATFIGNIAALFSAPIYIVIISLLKPDTHPFDLNLLGKDIKMADDYDQEEEEAIHVSEKDKKVLKKQSIFSIAVNFFILLLVYVMVACVLFGVKKDFSKSSFVAFIVVCLIWVVLAAIYIIVLPLYQGRHSLMFVLKHLVRFRIPDHPMNYTERHASEHPHVLDGDIETSSIEAVSYVHESKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUW7</accession>
    <name>A0A1E3NUW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA damage response protein kinase DUN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020571.1">
        <property type="nucleotide sequence ID" value="XM_019163797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUW7"/>
    <dbReference type="STRING" id="763406.A0A1E3NUW7"/>
    <dbReference type="GeneID" id="30180484"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="68"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="165"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="59235" checksum="31E4B4B9B91E9A3B" modified="2017-01-18" version="1">MEIPVEEVNSRKRELNVSFGKSPNIDPVQQFKRPKVATKEKEYGIFAVLSSLDTLKYENISIPFKEIVRIGRSGTKNNDIIISNNDCSSVHCELQMGKSFNHYNLITIKDLSSNGTFVNDVLVGKNNSALLRDGDRLSFASTLHYIVRYDLKYKSDRASFFDKYILTEKPLGTGHYAQVKEAIDRESGRICAVKIFNPTSQGGQATQLNRELEILVNLNHPNIVGFYNTFLEPVSMTSLTTFLVLEKVNGGELFNRIVKKGKLGQDETKEICKQLLDGLQYLHSMDIVHRDLKPENILLSIVPTEAGQSHNQPWDNGEASVQVKIADFGLAKLIGKSQFTTTLCGTPAYVAPEVLANSQHRKYNKSVDMWSVGVLLYVCLCGFPPFSEELGPPSMRQQILEARFAFYSPYWDKIEDISLDLISRLLVAKPDQRLTVQQAISHPWLQNFSQTENIEVQRLEKEASFDDMVSLRARQLSIRIPSTSRGYSCEPITIKERANTGLTDDIIAQRYCHYNKTDADGDTAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDI0</accession>
    <name>A0A1E3NDI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74786</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015290.1">
        <property type="nucleotide sequence ID" value="XM_019164870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDI0"/>
    <dbReference type="GeneID" id="30181557"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="58992" checksum="448EB1CC4522B7A8" modified="2017-01-18" version="1">MTISYTDEKHQKDPSVTEYEDEIQLGTVLSESGQEFEIHGNLKEMDQALELAMNNDDFIFTEEEDTKLLRKIDLVLMPFVSFLYSLFYIDKASNGWAAVMGIRKDMNMKGIMYSWTGSAFYLGYLVYQPLASLSLQKFPLVTCFSVYMFSWAVIICLQATNHSYPAFTFLRTLLGCSEASASVFTTLLTSQYYRKKEAFLRVSFWLATSGFGVFFGNMMAYGIAIRSESFSIAAWKVLFIVIGLISFVVAVILHFHVPNTPATAWFLNEREKRIVVERIRENYQGFGTKVWKKHQFFEAVLDIKTWVFFIYAVAFNIPNSALASFGSILLSTDFGYSSTDALLYGAPQGVVEFLCLPAIAYAIQKFNLPRLLSTAVVITICITFACMLAFGKNSGTKLAGYYLLYLMQIGPIGSFSYFASNVPGYTKKITVTSVYLVGYCVGSLVGPQTFATKDAPHYTPAKIAIVVCICVAAGCFYTLWLICYMENKARDKKAIDLGDKYCAPKNIEFSDLTSTENIYFRYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDL0</accession>
    <name>A0A1E3NDL0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015338.1">
        <property type="nucleotide sequence ID" value="XM_019162014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDL0"/>
    <dbReference type="GeneID" id="30178701"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="54" mass="5859" checksum="7176FF97D72FB1A7" modified="2017-01-18" version="1">MHVGIFTVNVMVHKHIRAEKSKKGYLRFASYLAAVIAAVIAANTKSQEAIESGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDZ5</accession>
    <name>A0A1E3NDZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_60504</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015470.1">
        <property type="nucleotide sequence ID" value="XM_019163441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDZ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDZ5"/>
    <dbReference type="GeneID" id="30180128"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="231"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="684" mass="77703" checksum="93000F82BA31E32C" modified="2017-01-18" version="1">MARVQGQATHKRRAGMLTITEDTFPAMLIWRCADSNATTNLVELPLDTVSFLQATPAHIDKMILKIGLDSGKEMLFGFNSRKVMDTVKTSIQQIIARKKTSISVEKEKEIDAAKANQDSGSVAPANGADGVKAAQASGSPDVPDDGVKNDEFLADLLNSKKLMKNLALQQRLLRDNVKLMRTFTEAVIKEGMEAEEFWKSRVHLLRSFAIQNNQKKGPYNVLSTIKPVASSDNEVNVNVTRDKIKEIFKQYPIVRKAYDDTVPRMSEGEFWSRFFSSKLFRKLRGEKVNLYDRGDVTLDKYLYFDPDYDGEEDVNEEEVGDHLLHEEKAQELPSHASSSSRAQKKKRKAGASEQMVKKKKVKLFNEVDSAVSKCLDLEGNKEDDPELRGNRPDITMKDDQDPELVGILRTMNRLSRRLMSDITEEAPVEDLDLEFEKELELNDLDETDEVEYNELKYTQTSNISSNIVPSELLTKVMNQSNLNPESCEYDDIIKKLKDEFTVEFDLTCVYRNMKQDGKKQSEAYRDINNIVKQNSKQSQQSWNSNLKELLGLPSSAATTSAANNSLTKENTDYDIGIKINLDSGKIESVKLTHATSIEFLRQFWTQFNRTAASTPTTDPNFKSDLIKLRKYYFSVKKCLSRVEAQLARCKDLKERQIENQMLTPLVEALNAALHRYEDSIVKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE51</accession>
    <name>A0A1E3NE51_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015505.1">
        <property type="nucleotide sequence ID" value="XM_019164825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE51"/>
    <dbReference type="GeneID" id="30181512"/>
    <dbReference type="OrthoDB" id="10541075at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="208" mass="21664" checksum="0212573059D905A1" modified="2017-01-18" version="1">MSLRRDLYKGRNSTPLAQGKSGSDENNHWVRDWYDPLAASKEDALLLNSNSTAAANSVLTNTTNTTASNTPEASDMAATPHDEKTPLTLTNEPAQLQGYEVKTWVISNNGSAAESYTDCDFASNPSATHIDLKQYRYPAVLRKGVNLTATSSNNRTAGANGDNDGDNGDDGDKAAGGDGISATDIKSAVGGTGVSGDTLFSSSAAIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE74</accession>
    <name>A0A1E3NE74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translocation protein SEC66</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_23183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015548.1">
        <property type="nucleotide sequence ID" value="XM_019162300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE74"/>
    <dbReference type="STRING" id="763406.A0A1E3NE74"/>
    <dbReference type="GeneID" id="30178987"/>
    <dbReference type="OrthoDB" id="73168at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018624">
        <property type="entry name" value="Sec66"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229:SF1">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09802">
        <property type="entry name" value="Sec66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21300" checksum="6EA2D935FC350127" modified="2017-01-18" version="1" fragment="single">PPITVSISMKTPLLYLGVLIVTLVVFSIYHRRSKIRSLQKLTSSSLFKPAYEVDESQITEYSLIAPTPTTDSTPALLYNDLKMLDGHDKLLKCSLVERAAESMRRIIKLKETEPSVMLLYTRGLIGDESFKRFKLQAKLQDAEMMEVAKEAEGYKQGWSRIIFANAQEVMMNQALRRRVGAINTRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE89</accession>
    <name>A0A1E3NE89_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 30 kDa subunit homolog</fullName>
            <shortName evidence="4">eIF-3 30 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HCR1</name>
        <name evidence="6" type="ORF">PICMEDRAFT_37086</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015545.1">
        <property type="nucleotide sequence ID" value="XM_019163057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE89"/>
    <dbReference type="STRING" id="763406.A0A1E3NE89"/>
    <dbReference type="GeneID" id="30179744"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="273" mass="29983" checksum="71F5CAE4E9D6F65D" modified="2017-01-18" version="1">MNFFHRVSDDEDFDVQVKPAATAPVSWEDEAEDDEPVLESWDVDSDEEREKKKKQADELAARKAEQKAKLAAKKAGKKEKTLLEIDLVDEKTRREMLKEAELNADLNNAADLFAGLGVADHPRARAAAGAGEQKSLTLTEDTPLTAHPLFHPTTKQDYEKLRKSLGTTMKELSASSTLFYANALAIDLARDICEPLTVEQIRKVISTLNATVTRKIKDERQSRLSKTGGTSLGGAGKKKVKTAVVNSGPASFKKDNLDDFMGGDGDISDDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NED4</accession>
    <name>A0A1E3NED4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_27206</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015603.1">
        <property type="nucleotide sequence ID" value="XM_019162487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NED4"/>
    <dbReference type="STRING" id="763406.A0A1E3NED4"/>
    <dbReference type="GeneID" id="30179174"/>
    <dbReference type="OrthoDB" id="21467at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12307">
        <property type="entry name" value="RRM_NIFK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46754">
        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="2"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="145" mass="16674" checksum="6E4EE8CB6CD43E2B" modified="2017-01-18" version="1" fragment="single">RGVIYIGRLPNGFEEKELRKYFEQFGEITRLRLSRNKKTGKSKHYAFLEFKEAEVAKVAAETMDNYLLMGHLLSVSVLDSDKVHEKLFVGANIKFKVVPYGKLNQLKHDKKKSKAEWEKLSKNHANSIKSKQESLAAMGIDFDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEK0</accession>
    <name>A0A1E3NEK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015673.1">
        <property type="nucleotide sequence ID" value="XM_019164729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEK0"/>
    <dbReference type="STRING" id="763406.A0A1E3NEK0"/>
    <dbReference type="GeneID" id="30181416"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070124">
        <property type="term" value="P:mitochondrial translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016712">
        <property type="entry name" value="Rbsml_bS1m-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28058">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP51, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28058:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11709">
        <property type="entry name" value="Mit_ribos_Mrp51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="365" mass="41037" checksum="7F4836EC1415C3E5" modified="2017-01-18" version="1">MLKSIHGPSAFGRLLKDTKIAQLQASIGSRELNANNGFPTHQVVESPASSFSRRDFGLKMRIPKKIKTRRIIVNDLDNKYGLPNFETLNGDYFKKLRFQELGIPVNATYTQFINSSNSSQNKTTTTQNPLFASSKEYSSPQNIAGLLHIKNQPLNSKQFAEDIKPQLKALRKPFYKWLVKNHPESISKKSLYDEFKRYIELEKPQLFGNYKNYIPSTYAEKLSGTGGLSYNLKGRLFQTPNGPKTSRVVPGRIVSKYSNYTKCTVGGFVGNVGNVAANNSYVETLALQNRSIEDDKFAREFKIPATVVNATMVLDNKSLDLTVNPIKRASNFGKRTITKRNPAEQSLAFSSLMNLLNDKEKTSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEY7</accession>
    <name>A0A1E3NEY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_22377</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015820.1">
        <property type="nucleotide sequence ID" value="XM_019162252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEY7"/>
    <dbReference type="STRING" id="763406.A0A1E3NEY7"/>
    <dbReference type="GeneID" id="30178939"/>
    <dbReference type="OrthoDB" id="6921389at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033386">
        <property type="term" value="P:geranylgeranyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="234" mass="26942" checksum="CBEE68BF8C6C9F15" modified="2017-01-18" version="1" fragment="single">IIQPYTYLKTQTRGKGFRNHLLEVFNIYYCVSVEELEIIQDFIDILHNSSLLVDDVEDDGTVRRGKTCAHRIYGVAHTINAGNLMYFKAWEKLMELPVEGLSKIFVDSMIKLHIGQGTELCWRNAKECPSEEEYLQMVVGKTGELFRLGVRVMACVQESRVGSKRPNNDPVTGLLEQYCDILGMIYQIKNDLENLQYEEHAAAEGLDTEVFAHDLKERKYSLPILYWLRRKGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFA9</accession>
    <name>A0A1E3NFA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VPS37 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_13472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015925.1">
        <property type="nucleotide sequence ID" value="XM_019160063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFA9"/>
    <dbReference type="STRING" id="763406.A0A1E3NFA9"/>
    <dbReference type="GeneID" id="30176750"/>
    <dbReference type="OrthoDB" id="3991664at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27252" checksum="8598FE91F6770573" modified="2017-01-18" version="1">MQPILPPKPHQDQQEQPTLESQLNRLKIDDNLSDRTDLPFKIIPSDFTTQLFTQKDGNSLNVPLPSYFEYITNAKIKELIYNEELLSGYLLTLYKEEFIGLKRELNAVIEGQQKAALELKNKYIDCDDGLLDKALALDTHLKEYDSKLKTFDHLQIEMYETLNGLSSKSIAKHFSDKAATNEANCSELLDNASSTEVELSSKQLEQLLQSYTTQRYEWHKNKECVSKLEHHKVVGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFB7</accession>
    <name>A0A1E3NFB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Arsenical-resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_73657</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015958.1">
        <property type="nucleotide sequence ID" value="XM_019164544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFB7"/>
    <dbReference type="STRING" id="763406.A0A1E3NFB7"/>
    <dbReference type="GeneID" id="30181231"/>
    <dbReference type="OrthoDB" id="187348at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015104">
        <property type="term" value="F:antimonite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004706">
        <property type="entry name" value="Arsenical-R_Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00832">
        <property type="entry name" value="acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057:SF1">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057">
        <property type="entry name" value="ARSENITE EFFLUX TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005508">
        <property type="entry name" value="Acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="45176" checksum="62F3D33A9277C88F" modified="2017-01-18" version="1">MIFLKENALRRAWSSLSITDKILPVAIILAIVLGVLLSVFVPSSRRAFDGVEVVGVSVPLAVGLIVMMIPPLCKVEWEKFHRLILQKKYLNHIVISLILNWIVCPFLMFGLAWLTLFDKPEYRIGIIMIGLARCIAMVLVWNNIAGGDANLCAILVLLNSVLQIVLYAPYQIFFCYVITGEYHKDSSNAISYSLVAKSVAFFLGVPLGFGLIIRLLASLTIGIQRFEKKVLPFISPWAFIGLIYTIIVIFIERGYSFIKDIRTALRCFVPLIVYFTITWFGTFFLLRWLLGRTAPNLEAFEDEDDEKEALLCGCEEEVANGSRTVSKGCSANYSEIITQTFTAASNNFELSLAIAISIYGSGSDQSIAATFGPLLEVPILLFLCFVAQLLKFNFEWSDINVNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFW5</accession>
    <name>A0A1E3NFW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17517</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016125.1">
        <property type="nucleotide sequence ID" value="XM_019161630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFW5"/>
    <dbReference type="STRING" id="763406.A0A1E3NFW5"/>
    <dbReference type="GeneID" id="30178317"/>
    <dbReference type="OrthoDB" id="2573163at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF21">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="195"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="405"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="569"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="715"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="454"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="599"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1071" mass="120648" checksum="EA3E133444BED951" modified="2017-01-18" version="1">MTDHTGEAAPNTRTELTKTQLSCDTLPNETSNDSLSSTKVTTKVVNNMAGRKAAKSLRLFRGSIAEPNVINSDASDSTTYHDSSLSNHQHREINIELNHPCTLKEKSSNHVIPDSPSPRKLSNDHNPTPTAIPEISAGLYINTQIPSNIKTTSHLRSESTDTHNVITLNKDQVSQTNPQPPLPLLEPVSSAIYFPHAAPNDSSNTSPEHLTAEAEFDHPENEEIVGDMEGLSEKSEESSTHKTSLKGTEEKTKDTVAQELKGNDAGGLTSIEDHSSSEVNRDNQKATNVDITLDPSRSEIMKCTSFTTAFSTSPDYSNERFVEKNSDDDIKYPLAVELQPFKNKVGGHTAIFKFSHRAVCKALVNRENTWYENIEILHPELLKFMPRYIGVLNVRYSTILDDSSEDLGPSHNGSVESSVTGNPSLNEPSAESDQLNNGHDFPKTHRNDVHNAKMTGTNKDTSNSIAKKNSLSKLNEDYEFPEVVLEDNIHIVPQSFWSRYTSPKIENSELINCDKVCHNFSFASPLDKPVTLSSPVQNDNQTGSTSVNTKLKELVLSEVFAPIRDYTKKARDSRKNSHYSNNPRDRRSSYQSDLAAEGSKSTFSPSSATSALSKYKSYDPNSGNLFSDPNTSTFPRFHRYSTSSMITSPTQKNEKSRHGKVFCNSITEGCPTEILEDEDTSIIGPSSVQSDDIKLHRNFRNDSSTAEQEMKFPDRETFMSNLKKLSRNKESEEDAIVDEEDADNVNESDIFDMEGVKDPVEHKSLVTGTNNERNRSIPEISYNGQENKTMQEGPHKHTLRKHTRFERFILLEDLTTGMKYPCVLDLKMGTRQYGVEAKPSKKASQRKKCYQTTSRELGTRICGMQVWDAKKLEFVNRDKYFGRRVKVGYQFFLSLSRFLYDGESIFSVVKHLPKLIEDLKELVDTFENLIDYRLYGSSILLMYDSELDRKNRDESTIIVRLIDFAQCVIGGSEFSDKTTFPPVHKGAPDVGYIRGLKSLIYYFGVMFREFTGGHEYASFSEAWEIIRKLDKEKVFNCNCQWLDEFDNGESQCPFEFRPVPVYTGFYDDVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFY3</accession>
    <name>A0A1E3NFY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="10">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_17549</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="10"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016160.1">
        <property type="nucleotide sequence ID" value="XM_019161640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFY3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFY3"/>
    <dbReference type="GeneID" id="30178327"/>
    <dbReference type="OrthoDB" id="9215500at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04300">
        <property type="entry name" value="GT35_Glycogen_Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000002">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000003">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF3">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE, LIVER FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="863" mass="98319" checksum="61881DD991D84F1A" modified="2017-01-18" version="1">MGPLDASVSTTRKNHRRTMTGFTPTEIKNIDAAIPMKCRNAWASHGVKAFNTADELETRFINHVETTLARSMYNCDDLAAYQALSSSLRDKLILQWNKTQQLYTIRESKRLYYLSLEFLMGRALDNAMINLDLKDVCNKSVDELGFNLEDIIEVEPDAGLGNGGLGRLAACFVDSLATCNYPGWGYGLRYNYGIFAQKIINGYQIEAPDYWLKFGNPWEIPRLEIQIPVDFYGSVNTVTDPKTGKEKKQWSGNERVLAIAYDFPIPGFRTNNVNNLRLWSSQPTAEFDFQKFNEGDYDDSVAEQQRAESITAVLYPNDNFYQGKELRLKQQYFWVAASLHDIVRRFLKTNKAWSEFPDQVAIQLNDTHPTMAIVELQRILVDIEGVEWNEAWDIVTRTFSYTNHTVMQEALEKWPVDLLGNLLPRHLEIIYDINLFFLQKVEKQFPADRGLLSRVSIVEESNPKNLRMAYLAIIGSHKVNGVAELHSELIRKTIFKDFVNIYGAQKFINVTNGITFRRWLRQANPDLSSLISKKLGSDEFLTSPVQLKKLEAFADDKAFLAEWDGVKLKNKKRLAALIKKLTNVDVNPSNMFDVQVKRIHEYKRQQMNIFGIIWRYLQIKQTPAEKRNKWLSKVCIIGGKAAPGYYAAKKIIKLINAVGEAVNNDPDVGDLLKVVFIPDYNVSKAEVICPASDISEHISTAGTEASGTSNMKFVLNGGLILGTVDGANVEITREVGEDQIFLFGNLAEDVEDLRYDHHLGRIDIPDALKLVFSELEKGTFGNYDEYRELVDSVRVHGDHYLVSDDFESYLEAQSTIDAEFINRDNWLKKSVLCVANMGFFSSDRCIEEYSENIWNIEPIGEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG14</accession>
    <name>A0A1E3NG14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016193.1">
        <property type="nucleotide sequence ID" value="XM_019161652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG14"/>
    <dbReference type="GeneID" id="30178339"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="66" mass="7403" checksum="00C5C125CE6B1CB3" modified="2017-01-18" version="1">MHCMNTTRSRKCTLTIVSDAETTSLVSISNSMASPKTPCFCWRHLRIASFHVFTSACSVPVLSYWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG86</accession>
    <name>A0A1E3NG86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoribonuclease YSH1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Endoribonuclease ysh1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">mRNA 3'-end-processing protein YSH1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17619</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016235.1">
        <property type="nucleotide sequence ID" value="XM_019161669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG86"/>
    <dbReference type="STRING" id="763406.A0A1E3NG86"/>
    <dbReference type="GeneID" id="30178356"/>
    <dbReference type="OrthoDB" id="10249535at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16292">
        <property type="entry name" value="CPSF3-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021718">
        <property type="entry name" value="CPSF73-100_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF11">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11718">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01098">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="12">
        <location>
            <begin position="20"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="13">
        <location>
            <begin position="253"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term" evidence="14">
        <location>
            <begin position="490"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="774" mass="86965" checksum="36A834334E473C43" modified="2017-01-18" version="1">MSADNEQLRFHCLGGGNEVGRSCHILEYKDKVVMLDAGVHPAYSGLESLPFYDDFDLSRVDILLISHFHLDHAASLPYVMQHTNFKGRVFMTYPTKAIYKWLLNDFVRVTNLSENVGDGSGGENANSFLYTDEDLRESFDRIETIDYHSTVEVDGVKFTAYHAGHVLGAAMFFIEMGGLKILFTGDYSREEDRHLSSAEVPHKRPDLLITESTFGTATHIPRLEREHKLTKMIHSTIQQGGRCLLPVFALGRAQEILLILDEYWQEHKDLQKYPIYYASGLAKKCMSVYERYIYMMNDHIKAKMAETNTNPFFFKFIKNLASMDRMNDSSPCVLIASPGMLQSGLSRRVLEKWSPDPRNSVILTGYSVDGTMAKNIVREPSEIPSLANSENMIPRRISVEEISFAAHVDFEQNAEFIDLVNPKTIVLVHGDSNPMGRLKSALLSKYQKLRSTDKEIKIYNPKNSTVLSLEFKAQKVAKVVGQLASALPDDGDQIKGILVQKNFDINLLTVDDVRAFTTLSTTIITLKQSVRCNANRSLVHWQFSQMFGFVKVLLDSEDVYEFEVMESVRIRHNDKTYVAHVEWQCGNMEDTIADTAVAILLSCDSLPASVKLTSKSCSHNHSVPEQEHFVKEVSTQADEENKVYVKKEPNSGSADTTGITIKEDPDAITIKTEKLSSPSSFQSASSSVRPIYVEHCDIAPQTRLARISQMLEIQFGKAFEHNVKESCGIVRIGKNEAVINYDDFSVACKSGVLKGRIEGILSRSLDLVSPLSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGE5</accession>
    <name>A0A1E3NGE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aminopeptidase</fullName>
            <ecNumber evidence="10">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_73994</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016333.1">
        <property type="nucleotide sequence ID" value="XM_019164641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGE5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGE5"/>
    <dbReference type="GeneID" id="30181328"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="13">
        <location>
            <begin position="10"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="11">
        <location>
            <begin position="255"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="12">
        <location>
            <begin position="562"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="916" mass="104134" checksum="CB36C027B55399D5" modified="2017-01-18" version="1">MSLLFCNRFVPKQYSVSLALNDTKPNYSGNVSIELVKNDKFHGDGDTADYFSVSLNTVEVVSVSAVLRCGDKAWKLLPTLNKTAESTEYSCKELPLRQLADTACLTLEIRFIAVVRKIMTYSDVTKGVFSTKYTDPSTGKSDLFLLSTHSQPHFARCIFPCLDDVNCKCPIQLELTADNRFSCISNMPVESSEYLSETSNKLVRFAQSPPMSTSVFSFAVGNFDFLEQTVRMPVSKCDFPIRVYTMVGESQRAQYALSTVAAAVGVLEQKFNVKYPLPKLDIVALPFLSDGGVENWSMIQVINDHILLPDWKVTDAHLAKIHRTIRDVLVHEIVHMYVGNYVSFDSYDHTWMNESFATFMSNTIINSVFDNTTWFNFLNSDLQQLKVRNMSADSAPIFTANVATSRIHDTFSRNSYEKGIFVMRSLASLFVENDDQLNQQNYDVFFGMVGDFIKKNEFGNFKPVDLWNFLKAHKSNRFGYDIPTIMNSWIRTSGFPILTVTKQDDGSYKIEQHHCLDEADADIEDVPFQIPLLIKKSDGTLGRQLMTDRSLVISAEDLSDDFFFLNANDSFIATVIYPEATTKEIASKFHVLNNVEQMQYFKNFSSVIGTHYQSNESITSFFTAIKYIKKVSRPDHAAMTFALSVLSNIYKSINTLACFNDAAFYKKLDTFINDLSNKYVAQLYWDNVDWKVLGTEELRLRNAILSLKYDNPATQVVGKTLFKKVLHGPKESVPAELLTTVFAIAAQSSNLKEYKEISKLVRNPGLVVNNVFPSVPNDVQTAAINSLGCMRDPELRYKTLNFVATNPDVKMIELALLGFRFQMSAYGELWKWYLQHYTVWYSKYARDNKSYQGLFFKHVSELALECAYYSPQLSKEVENFVASKNDDVKTWFEEAKAKLDSIRLLSEANQDLKLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGE7</accession>
    <name>A0A1E3NGE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17677</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016302.1">
        <property type="nucleotide sequence ID" value="XM_019161690.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NGE7"/>
    <dbReference type="GeneID" id="30178377"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000296">
        <property type="entry name" value="ATP-dependent DNA helicase RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000340">
        <property type="entry name" value="Bloom syndrome, RecQ helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="857"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1056"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="32"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="194"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="440"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="494"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="798"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1367"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1558"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="456"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="494"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="548"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1381"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1568"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1600"/>
            <end position="1613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1623" mass="181570" checksum="4701D5B8144CED55" modified="2017-01-18" version="1">MPPTWNLKTQLEWIRSSKPYVPRVDLAVPDLTSRQEQEYRTAGKAPLARDSPTQRWNTTGAGTAADTLPGSHRPGPSSLASSSTTAAASAVAGTGTTTTTFTQLTLPLRHSLHADAQEKSRSSPSVNQDANPNSNGNAFANANTSHSAAAAAATPPTSIRGQIQITQLMQRRSNTTGNTANTFIDLTQDVVPPARAANPAPAASTPTLPQKRKLEDTGVGQGSNFSNKRRTAEVDDTDVFSDDEEVEAILAERESSEVSKKTSFSASHILLSKDIGERSPLASNPNTLDGDPNEHRFTMEQLIELCQKQSQLIHHLTHFNDFLESGIKVETSTSLSEDCKRQKRRIFNPKHQELRAKCSRLAQKIPKINLSTAVSNTQTILTYSDNSNTNGSFVNTANHADVASQNDRLSEGFSKIASENAQNIHDIPMDESFSILEDNVSTARETDKERQREKEVESDEDDNHDEDDDDTDIIIHNSDPKITKRLDTNSTFLSSPSCTTNTNTSISHSVLNSNEKHNDMIPPSDVDRRSRPSRVLQPVLPPPVWSTQEDDIEDSFNCESEGEPETKTQIRKEMGNFVVSDDDGDVSVDDSYKEPEWNSDNEIDEIEDVDNSDIVQLRNTEIENIDDPDEPPTTSKSAVLDDEICICDSDIEDDKLVFSDALDNLPDKEPICLSDGLNEAEKSTENEGWSIAHSTHKADEHHAQLGIHMNNEEPNGIIQPAFTNERENDEALEDNDDDDDDDDIMIINNGTDYFTQLDKERGIDFEEKNVDDDLDDDFLWDINDADLIKNLEAPPSATVESTISKEDEEEDVEELTNPNKDDFRGYHGKHEWTGEIYKALKNVFKLQSFRENQLNAINSTLAGDDVFVLMPTGGGKSLCYQLPAIVKSGVTSGVTIVISPLISLMEDQVSHLEHKNIKASMLNSKMSADERRHVYNLFINGYIDLMYLSPEMISRSGQCRRAISKLYSDGKLARIVIDEAHCVSSWGHDFRPDYKELKFFKEDYPDIPMMALTATANEIVRSDIIQHLGLNEPKFYKQSFNRTNLFYEVLSKERSVMEDIVSMINSKYSGQTGIIYCHSKNSCEVTAQRLCEFGIACQFYHAGMETNDRTQVQKDWQEGKVKVIAATVAFGMGIDKGDVRFVIHLTIPRNLEGYYQETGRAGRDGQHSDCIMFYSMKDARTLQGLIKKDRDLDAQSKENHTHKLKQVIQYCENHIDCRRQLVLQYFNERFERKECLKQCDNCQELDNVEVTVKDMTKLAHACVSLVKEISQRKVTMLLCQDIIKGAKYAKIVSGGYDKSIHHGAGRDLSKTDVERVFFHLVYNDYIQEKSLMTGGRFSSNYVYPGQKARRLLDNKEKIMMEFTNKKSGKGRLAKNPAPLARQSSFRPSSELWSGNTRNSFANNSDREPGKNFTGNGIMAGVSAKGINLDILSLTDPAKKSHLNECYQELRRERNLISTRLGLNKEATLASDTMLKDLSLKLPKNVVVYKQLEDFKRDQEKYLPHFTKKINQLRDKRMLLFGTLNLTSSEAYDFYESNEVLDITEDSATMSGCSTTTSRFFQGAETGRRSGRSAGRGRRGGRGNNFRKNGGSQGNRKSKKSAGNVNANATGSGSKHSHAMAMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGI0</accession>
    <name>A0A1E3NGI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015791.1">
        <property type="nucleotide sequence ID" value="XM_019161872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGI0"/>
    <dbReference type="GeneID" id="30178559"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="452"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="104"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="273"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="704" mass="77798" checksum="C86C1A10164BED2B" modified="2017-01-18" version="1">MGFINKKHDKKERLERMQELYKSITNSNWAALSMEQNGHFKHALISWTDLNSFADEQVEKLNEETANVHLSTDEKIILDELNKLRAEIESNCYASAKAMERHLQLQHQQSQHQQQLQQQHQQHYGNAVPTLRTSIPHTMSSRKPGSKMLKSLRPGGLTSYSSTPSATSMSKQSGVPSASTGSSVSNSLPTAKHAANLMWAPSNSLNVNNSNNNSNSNSISNSNLLAPDSNYTSNSYSNGSVNNNEYQSNSVSATISPAGEFAEFSSTTVATVNTNGHSKKTPSDRARAALKASAAVKYPNRPELSNNSNSSSTNKLDATRQHRSHRSRSPQYEISPNPPSSTSYSPVASESSLQSPTRAIKNPKVPVAAGDEIKADEDPAEDSEEDEDEKILKKIKGVDLKFAKNILVESSRDQDKVFWSDIAGLEQAKETLMETVVYPFLRPDLFRGLREPVSGMLLFGPPGTGKTMLARAAATESNSTFFSIQSSSLTSKWYGESEKLVRALFQVAKAKAPSIIFVDEIDSLLGKRSGDGDDDTSRRLKNEFLVQWSDLSKAAAGRVDDENDPPSRVLVLAATNLPWAVDEAARRRFVRRQYIPLPEPETRLAHFTKLLMHTPNSLTEDDMQQLVELTEGYSGSDITSLAKDAAIWPIRELGSKMLNLKEVEIRPLCLGDFERSMKNIRPSVNKEGLEKFEEWAREFGSSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGN9</accession>
    <name>A0A1E3NGN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016410.1">
        <property type="nucleotide sequence ID" value="XM_019161740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NGN9"/>
    <dbReference type="GeneID" id="30178427"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000080">
        <property type="entry name" value="Aminotransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858:SF1">
        <property type="entry name" value="LD15494P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="2">
        <location>
            <begin position="53"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="440" mass="48924" checksum="91A913CFA109104A" modified="2017-01-18" version="1">MSHQKIAAYNFFKGHPSTDLLPAEDILKASEKVLTTFNKTLAHYDGQNDTHPLNYGPDLGNLEVRALIAKWNDNLFKLEEKTDPDCINLTNGASFGLMNLLLQCTTPLHNVTKRIFLISPTYFLINSAFIDNGYAGKLSAIRELDNGEIDIDEIERQLESIESETPSKKDITKEDVPEIYDPYRPVKKLYNYLIYIVPTFSNPKGGTLPLESRTRLIAIARKYNVLIVCDDVYDLLDFNAGKENHSYHKKMVYLDRETLPEGEEFGNVVSNATFSKLLGPGLRTGWQETATPKLANLLASGGANKSGGTPGHFNTTIIAELLKSGKVDKIVANLNAVYSRRAEVLKAAVRKYMPEGTSISPVDGGYFAWVTLPEGFDNLKIAKECANHGVVLATGDNFEVTGDPLGWGEHGVRISISYLDETQIEHGIKIWGEQCQAFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGU2</accession>
    <name>A0A1E3NGU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016470.1">
        <property type="nucleotide sequence ID" value="XM_019164683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGU2"/>
    <dbReference type="GeneID" id="30181370"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF275">
        <property type="entry name" value="LD06095P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="395" mass="44927" checksum="39D9A412D6121D95" modified="2017-01-18" version="1">MSEAQLFISTFNCGKKTPTNAEFTKHLNEKLPEEASDLYVFGFQEVTSILDATDENVVNKKLIWIADNLITCLENRYEMQSFAVVSIAHFGAVGLIIVSPFISKIHKIYKSRGYPVGTFCTNLKGGVGVRLTYESAEFTFVCMHLNAGEKVQHMLRRNQDLYNVFSKLIFDDGWCVLKPDAHCFIFGDLNYRATGAFGLVPKGLDFDETENREGTENCDPAFIFKKDELSLLQKGGLILQDFEEPAICFKPSYKFHIGTKNYHRNRIPSYCDRILYLGYPESSKTPKYKAVAYDTITECLFSDHLPVYMIVNVSLTAPERIINDAGYLIDRTTQLNEFSKSYKAVGYSRYILRVSSFTTSMLGTLLYLSTTNRGRMVSLAFAILVYTLLKWKELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH15</accession>
    <name>A0A1E3NH15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dynamin GTPase</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17888</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016534.1">
        <property type="nucleotide sequence ID" value="XM_019161793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH15"/>
    <dbReference type="STRING" id="763406.A0A1E3NH15"/>
    <dbReference type="GeneID" id="30178480"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034643">
        <property type="term" value="P:establishment of mitochondrion localization, microtubule-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097753">
        <property type="term" value="P:membrane bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090149">
        <property type="term" value="P:mitochondrial membrane fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140572">
        <property type="term" value="P:vacuole fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000002">
        <property type="entry name" value="Dynamin-1-like protein isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="25"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="13">
        <location>
            <begin position="704"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="551"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="574"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="614"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="791" mass="88487" checksum="373BDDAFEA5645B2" modified="2017-01-18" version="1">MAALQELIPVVNKLQDIVTNTSLTDLDLPLLTVIGSQSAGKSSVLENIVGKDFLPRGTGIVTRRPLILQLIHLDDDDPLLSRADYVADEPSEPSEMTLEQHLRSQSSSNEINGKAAGSPPPAEWGEFLHIPGKRFYNFDNIRKEIENETSRIAGKNKGISRIPINLKIYSPNVLNLTLVDLPGLTKIPIADQPVDIERQIRNLILEYISKPNSIILAVTPANQDLVNSEALKLARQVDPQGKRTIGILSKLDLMDHGTNALDILTGKVYPLKLGFIGVVNRSQQDIMSRKSLNDSLAAEMEFFNSHPAYKTISARCGTAYLAKVLNKTLMNHIRERLPDIKAKLNTLMGQTEQELASYGNLNIVSKENRSGLILQMMNKFATSFMNSIEGNSSEISTKELCGGARIYYIYNEVLGNSLMSINPLQNLTVIDIRTAIRNSTGPRPSLFVPELAFDLLVKPQIQLLEGPSHRCVELVYEELMKICHNCGSQELARYPKLHAKLIEVVSDLLRERLGPTTKYVESLIEINRAYINTNHPNFVGAATAMASVVEERQKQKEHERDHEKHLLLKREESATAPAKVAKAAASTAAANAGNGSASAESKEHSAKSNNKPESSVLDAISSSSHSLTSESRPEKENFLNFFFGKKEEVQGKVEHNKIAPFSYPTETESSNLQFHLPIEDSIQHQMDQFHLNEEQLSEREQLECELIRRLIASYFSIVRETIRDQVPKAIMCLLVNYTKESVQNQLVQKLYKESLFEDLLYEDEALIQEREKCEKLLDTYRQAATVISEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHC8</accession>
    <name>A0A1E3NHC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_141070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016647.1">
        <property type="nucleotide sequence ID" value="XM_019160227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHC8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHC8"/>
    <dbReference type="GeneID" id="30176914"/>
    <dbReference type="OrthoDB" id="3996649at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018555">
        <property type="entry name" value="C630.06c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09428">
        <property type="entry name" value="DUF2011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="36277" checksum="CE395F95C2CA9716" modified="2017-01-18" version="1">MVFQHAHQSQTDFHPPLRGLLPPRPGQHSPERPMSDVKTVSRSQLYESGGEDAASSDEQEYNCPPPGFDFEIVDVDDDVDADQPPAAEAGDAGDKTDDSTSLAKDGDDKVEYFPLFSTTATPNGASLTNSSGTAGSLVRIKVDDLADVDEEQEPGKVKDEEWDQIAKERNESRRPLSYYYHDGDGDDQVEKYKAVAVDGDTVIQWSKHFRILTNYRVKDLAKHNATVDLYHKIDRSARDGKGKKRGGKKQRDAKIFRKERLKEWKQKLAEARERARQRSYREDPAATAGATSSTKTKFGDSFRKRSNTVSKNIGRPGKPVFRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHV7</accession>
    <name>A0A1E3NHV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Very-long-chain 3-oxoacyl-CoA reductase</fullName>
            <ecNumber evidence="12">1.1.1.330</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">3-ketoacyl-CoA reductase</fullName>
            <shortName evidence="12">3-ketoreductase</shortName>
            <shortName evidence="12">KAR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Microsomal beta-keto-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16993</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the microsomal membrane bound fatty acid elongation system, which produces the 26-carbon very long-chain fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty acid elongation. VLCFAs serve as precursors for ceramide and sphingolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="EC" id="1.1.1.330"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.330" evidence="12"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016817.1">
        <property type="nucleotide sequence ID" value="XM_019161419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHV7"/>
    <dbReference type="GeneID" id="30178106"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141040">
        <property type="term" value="F:very-long-chain 3-oxoacyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000317">
        <property type="entry name" value="Very-long-chain 3-oxoacyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03107">
        <property type="entry name" value="3_ketoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027533">
        <property type="entry name" value="3_ketoreductase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086:SF2">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="349" mass="37492" checksum="95C3544B039F5104" modified="2017-01-18" version="1">MLDLFESFCNCDCSVVKGFLLAAFAVGVFKITTLVLSLGTLLVQAYVLPAVSFAKYGGGKGAWAVITGASDGIGKEYALQLAERGFNIVLVSRTQAKLELVAAEIEGKYKRQTRVVAFDAAEDAPENYAVLAKALEGLPVTILVNNVGQSHAIPVPFLDTSEEEMMRIVTLNNIVTMKITRVVAPVIVANVGKATKLRGLILTMGSFAGLVPSPLLCVYSGSKAFLQNWSTSLAGELKPDGVDVELVISYLVTSAMSKIRRASFTIPNPHRFVASTLRNVGRRVGAQERYATVTPYPAHALMHWGIENSAGVYSKIVNKINFVMHTTIRARALKKAARAAAADTAKKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI97</accession>
    <name>A0A1E3NI97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016988.1">
        <property type="nucleotide sequence ID" value="XM_019161477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI97"/>
    <dbReference type="STRING" id="763406.A0A1E3NI97"/>
    <dbReference type="GeneID" id="30178164"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="3">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="149" mass="16777" checksum="C5606B4DA54A142E" modified="2017-01-18" version="1">MSQEDIYLQSYPFTTAAAIIGFVDRKVNAVLIDGRAVMGVLRTFDQFGNLVIHDGIERIYCPETKQYAESTHPQIYVIRGENLLMLGDLDIDTEDTAIKGWERLDYVDTYNKRRKLIEEAKAKCTQETKKLGEVGAVVGGGFVYNLHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIC3</accession>
    <name>A0A1E3NIC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="4">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73377</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017000.1">
        <property type="nucleotide sequence ID" value="XM_019164464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIC3"/>
    <dbReference type="STRING" id="763406.A0A1E3NIC3"/>
    <dbReference type="GeneID" id="30181151"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19120">
        <property type="entry name" value="AKR_AKR3C2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044494">
        <property type="entry name" value="AKR3C2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="10">
        <location>
            <begin position="51"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="9">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="315" mass="35615" checksum="A96F5EE33486C933" modified="2017-01-18" version="1">MPALTIPTFTLPGTGDQVPAVGFGSGSKHRLKKWGNPEMNDDSIDPGIVTVIADAINAGFNHLDGAETYTTRAEMGLAIKESGVARKHLWITDKYDQGWANFKRASTSPSGPRESILKGLKALGVEYYDLFLIHSPFFDENLVNVTLAEAWKQLEQCYEEGLVKNIGVSNFDVENLDKLMKVATHKPQVNQIEYHLYLQNQTDGISDYCRENNIQIEAYCPLTPALESRINDANHPLKPEFKKLCEKYNVEPSVISLRWIYQSGVIPITTSSNAERMKQAFQMFDFELTDEEFDTLTKIGKSYKVRAYFPELFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIH5</accession>
    <name>A0A1E3NIH5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="4">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_17144</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016999.1">
        <property type="nucleotide sequence ID" value="XM_019161481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIH5"/>
    <dbReference type="STRING" id="763406.A0A1E3NIH5"/>
    <dbReference type="GeneID" id="30178168"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="10">
        <location>
            <begin position="45"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="9">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="315" mass="36022" checksum="38AD9DDBB6537A30" modified="2017-01-18" version="1">MTLAKIPTFTLARTGAAIPALGFGSGSKHRIKKWENAEKTGEAPLDADIVDVLSNAIKAGFTHLDGAETYTTRGELALAVRNSGVPREQLWITDKFDQGWPHYDRKSPSPSGPRESVLKGLETFQMEYYDLFLIHAQFFREDLVGLSIVEAWQQLEQCYEEGLVKNIGVSNFDVQHLQQIVDAAKYKPQVNQIEYNLYLQNQTEGIADYCRKHEIQLEAYCPLTPILDARINDENHPLKPVIREMADKYNVSPNILALRWVYQSGVIPITTSSNFERMKQTFQIFDFELSEKDFNTLKSIGQTYTVRTHFNQYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NII3</accession>
    <name>A0A1E3NII3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_59519</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017037.1">
        <property type="nucleotide sequence ID" value="XM_019163373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NII3"/>
    <dbReference type="STRING" id="763406.A0A1E3NII3"/>
    <dbReference type="GeneID" id="30180060"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF29">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="56413" checksum="6562E7526B05F75B" modified="2017-01-18" version="1">MSSKDEYTQEIELEASSEDGLTNDEKTLIRLGYTPEFKRELGLWSIFSVSFSVIGLLPSVAATLFYGVGYIGIPGISWDWPIACSGIICVSLAMAELCSSMPTSGGLYYAGAVLAPTGWKALTSWTIGWSNWLAQITWAPSVKYTLANLILALAQIKNPDYEPQKYQVFLLALLLLFIHGILGSVPTKYVARINSIGSCLNAAVIIIGIVMLLVGNKREEPKWNSNYQVWREFKNNTEWPDGIALLMSFLAAIWCIAGFDGPFHLAEECSNASTAAPISIVLTTVLASVFGWVLMLIIAYTIVDVEAVISSDIGQPFATYCDQLMGTDAATALLALIAICLFFTGQACVVTASRVAYAYARDGCFPFSKYWSQVNKTTRTPVRCVWGNVSIGALILLLTFNSEAISAIFSLGGTAAYIAFTIPTLLKLFAQNKFKPGPWNLGKFSAPVGIYACSFVLLMVPILSFPQVRGVDLSAQVMNWTSLVYYGPMAIALLWYAIDARKWFQGPNVSKDHFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIJ8</accession>
    <name>A0A1E3NIJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_167393</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.108" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016286.1">
        <property type="nucleotide sequence ID" value="XM_019161324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIJ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIJ8"/>
    <dbReference type="GeneID" id="30178011"/>
    <dbReference type="OrthoDB" id="377083at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004168">
        <property type="term" value="F:dolichol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="582" mass="65318" checksum="F1212C239A722DA8" modified="2017-01-18" version="1">MPPKKSKSKKNAIASSSVVHRQVKHKTTALDYDYDTQVESVTSEADQKPAPPISAPPKNIEEVGDIKSGIVSYYMALVDVSINTRHVFQIFMLFFLTNLIYLHFKTQEDDGSTDAMEKMITASCIVLAALLECCAVLNSRFRQFESGKTKVKPLLLDFNYVYSVFFPLAVCLTKASDKLVLVSCCVVQIAYMNVFVRCLISYVILFQFGDGDSFNGKSLILPLASCFFYEAIHKFVGSEIEFSEKSFLSVILAMGSFLISYNDSNLTLFIMRNLYLSFIIGIILASPILDLYKSQTERTLKYTWLLGIYILFFSAGLIISDKLLLPSLGKFHLNWLIEFVSASEQRSRIFKQWLIASSILVPGIFVIFSKVNVSMSLKRKIWHFVLLMLLAKPMIVEPELVSIALFGILGILIILEIVRANDLPPFGGTLRRLFSTFQDTKDNEGQFVLSYIYLVLGASLPLWVNNVDSLQESTYMGLLTLGLGDSIASIVGSRFGRHTWPETNKTIEGSVAMVVGMMGGYALLDFLFQACSVQDKIVVLSWTNRFMCAVLCALFEGIVDVNDNLFLPVFGYLVEELLMFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ09</accession>
    <name>A0A1E3NJ09_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_153145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017238.1">
        <property type="nucleotide sequence ID" value="XM_019160762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ09"/>
    <dbReference type="GeneID" id="30177449"/>
    <dbReference type="OrthoDB" id="4026687at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="679"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="859"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="922"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1247" mass="136702" checksum="C6357AA97C7A1D4A" modified="2017-01-18" version="1">MSSNRKKRLSLIDFFGKSSDTAPKSGKKHQRSSSDTSKISAKNNPHRLTFQIPDESIVSKVDLSKIPKPPVPAKNRYAGLQNTSSSHTNANSSSNNSSRVKSLPATTSSTNGIALQKENSKLPETSREKPEWQRSSPYHSNSQPSEKRQVSNSSLSSSMYPNELSPITSLRNTSNSTLPDFSRQTSSIYPASTFAQDSPHKNYLESSGTISKRTDLLRKRRPPPPSLKVKISDPSMTQPELQSPSDTMKASAADSNHLSEYDSTDGEFTPLNLIPPTSIPAAMKSGQLSADKLLNPSKNVMFVNSTTSYEPPNFHSEDYKYNSKDQYQDKNVHKHSRTLSSIEEITSALDSFQLEHEKSFMSEGSQGRMETEGDTDGDAAGGTLSFTSSPVSATERPIALHLTQDLGKSNLNNDLAAENGATDENNGIFVKVKDLPHLQNGETLDQYISKLKTAAPDSYLSADDNNSEKTHFLELEKPKSVGGGSENSSASDVFFDVDEPSKDASLDGNIHRATFDEDVGDSPEQQASAVNNMSISGNKYHSGEARSLQPNGFADLSAQDIDDDVENEVLDDYSEEDLIGPLDGSFESLVHHAKPVSIAEDEKYISGDVIAETQTISTVASNIPESIEADDGVWERYSSATNFFNNADELHDGKIVDDDEEGNDRDALYGVKNVSTHSDSSRSLILTPTDSRPYVDMGMNLSNSSPHRDFDLGDELENNVTAVLPETSSSRSLEDLYPTHSSDGGNEDDDDDDADDDVENDDDENNDQNDDDNYDRFEGYDSQDNDNMSYIVDDERNHMDIGNYTSGEDSFKQQQYSMKNKAVETNDLTPTDFSKGAVASGESSSEENDDDDDGVLEFSPRSPRSPRSVDSIEEMAQTPEIFRHNSMCSKSLLEKASGINLTGTPTGLGIGPRQHLVITNQEQRSLDENSLSNRNSDEIERAPIPDLESNFSYESRPHNLPEDDPENDNDNHQIIYDRREEPTVMPIQHSLVVTPAAPVIGTSSTLKKRRPPPSYPSTGRRGRGLSETTANPELFANGIAQQPKPQSNDNESSEAISRGFITVAKDQAETSVAEGGKAPVIPEQEEVNEELESRKGEKCIYIENLRLRSKKTAINTKPSVQVLPLAIRQNGTISHKKIPSQQLTHSFKSNKHMMAKPKTRMLASEINDGELPDATLIHRGQKTKVPIHPDAAVISASEQFSKLAKQQAGTSSGGGGDLGRFNSVLSVRPHYGNGMRLFITNPDSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJD9</accession>
    <name>A0A1E3NJD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sas10 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016576.1">
        <property type="nucleotide sequence ID" value="XM_019161810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJD9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJD9"/>
    <dbReference type="GeneID" id="30178497"/>
    <dbReference type="OrthoDB" id="1924577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="54761" checksum="8169233E1E62D190" modified="2017-01-18" version="1">MARRARSFARAEEKKNEETGIEEFGMDEVDAFADARDKSLMEKAGLNEQPYDANNSEDEEAVEGVMDIDSDAEVEKYKKKFQGPIDESDEEYFRAEDDSGAEGEDAEGRERWGGDYYGADEAEDEEDEKLLEEEALRLQKKHMEELNVEDFMLDEVEDWESEKEKKRKQKQVGAGDEGDELLTENDDKFAEMLKKGDKQSRTEMINKKYPEYLPLVLELKELQGVYESLKSSKSESVIKEVQFKALSLYLGSIVSYLAVFASKLQSGDKFDMKDEDVMVSILGSRELWRQAKGLRKASHITVEEDQEYEEDAEDQESVESEFAEESLSNKKPNKFAKEDLRGSDVSEEEEEEEEEEEEEEEEEEEEEEEEEEEEEATVSKPQEKTPDFTKLRSIKKSTRKNLEDMDDLDVEDKQGRRKTLGFYTSKIDKRDIGKAAVDGDVDAAYEEESSNKNKHYAQKRRSQPSQRGRRRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJJ1</accession>
    <name>A0A1E3NJJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphingolipid C(9)-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.317</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_16189</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.317" evidence="14"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017393.1">
        <property type="nucleotide sequence ID" value="XM_019161119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJJ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJJ1"/>
    <dbReference type="GeneID" id="30177806"/>
    <dbReference type="OrthoDB" id="412182at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052290">
        <property type="entry name" value="Sphingo_C9-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197:SF1">
        <property type="entry name" value="SPHINGOLIPID C9-METHYLTRANSFERASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197">
        <property type="entry name" value="SYNTHASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G04190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ46280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="492" mass="56930" checksum="AAD8204FA2AB6442" modified="2017-01-18" version="1">MEALKNYKCGVRTTNSPAIKNAPLPADGAGSKHFNNWVLLAIMLFIPFQLTRTVGLGFKTFLFFFLVLAIPILMAWWTLLSTYSPRLNEKAKFPNKGVEYYLDFHDEELAAKYKGLNKIPMETFHELYFANKVSFKGDALDVMEFRHDWAQFRFTLGLFKFFLFGFVPEVIMHTRSQDEEQVRDHYDRGDDFYEWFLGPRMIYTSGIIHNINVEEPLEQLQDNKLKVVCEKLVVKKGDNVLDLGCGWGTFASFASSQYGAQVTGVTLGRNQTEWGNNMLKKYDVPSTQSRIVCCDYRDAPLPASAVEGEKGKYDKIVCLEMSEHVGVRHFSSFLKQVYDMLDDDGLFFLQYAGLRKSWQYEDLIWGLFMNKYIFPGADASTPLDFVVGSLEATGFETVSIDNIGVHYSSTLYKWYLNWISNKEKVVNKYGIKWYRIWEFFLSYSTIISRQGSATCYQIVLRKNLNSYHRIEYVPHQEGIQGPASRFLAWFRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK17</accession>
    <name>A0A1E3NK17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017605.1">
        <property type="nucleotide sequence ID" value="XM_019164233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK17"/>
    <dbReference type="STRING" id="763406.A0A1E3NK17"/>
    <dbReference type="GeneID" id="30180920"/>
    <dbReference type="OrthoDB" id="20681at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192">
        <property type="entry name" value="NUCLEAR PROTEIN E3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19476" checksum="884ECA94DE125D5E" modified="2017-01-18" version="1">MHFRRLLHTTARVSRHYTTTFKASNLGQNLAKYDIYALADKPENNVEMISDDSIVFSNLKVVRSPAPGQGQPVGALLLNNQIFELSLEGIELQHNGVVVELGEAVTDVLGLVYPKPELLVAGLGKRTRMLGPKTKERLSQLGIRVETGDTRSSALSYDLLATERSPALVASIMLPPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK51</accession>
    <name>A0A1E3NK51_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_131474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017635.1">
        <property type="nucleotide sequence ID" value="XM_019160021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK51"/>
    <dbReference type="GeneID" id="30176708"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="58" mass="6610" checksum="A7DD74D90655ADEB" modified="2017-01-18" version="1">MRKMATDKERRRGSRGTESFHERRACREGPGEDVLAEDVLADDVSEKTVFSRLRGIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK61</accession>
    <name>A0A1E3NK61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mago nashi protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_33622</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mago nashi family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017645.1">
        <property type="nucleotide sequence ID" value="XM_019162830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK61"/>
    <dbReference type="STRING" id="763406.A0A1E3NK61"/>
    <dbReference type="GeneID" id="30179517"/>
    <dbReference type="OrthoDB" id="6495301at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1560.10:FF:000001">
        <property type="entry name" value="Protein mago nashi homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1560.10">
        <property type="entry name" value="Mago nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004023">
        <property type="entry name" value="Mago_nashi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036605">
        <property type="entry name" value="Mago_nashi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638:SF0">
        <property type="entry name" value="MAGO HOMOLOG, EXON JUNCTION COMPLEX SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638">
        <property type="entry name" value="PROTEIN MAGO NASHI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02792">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89817">
        <property type="entry name" value="Mago nashi protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19549" checksum="A0EFD19019880CA6" modified="2017-01-18" version="1">MPESFDIDPLQLDTEFYLRFYTGGYRDTGYGHEFLEFDIRHNPGDNYATLRYANQSHYRDEELIRKQVCVSMLVLETIKTIVRESQILLESDLDWPDPLKESKQELEVRSGRNIKRFKTKNISSLVSIKSQKDSQGLSVFYYLVQDLKVFVFSLISLHFKINPLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKA3</accession>
    <name>A0A1E3NKA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017629.1">
        <property type="nucleotide sequence ID" value="XM_019161186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKA3"/>
    <dbReference type="GeneID" id="30177873"/>
    <dbReference type="OrthoDB" id="5364312at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="587" mass="62150" checksum="476724BD0C9C94C4" modified="2017-01-18" version="1">MFSPPSSTDYDDSLGNRPGGRGHGHGSGNNSSSNHFGSFSFSDPSSAAASASASPVSSVARRKASKPAASKNIDVPAATIASNPTHSSNLGIHSPLSLESAKSNDSQDPGADAASSHAAESVYYVKHKGGPGSKSATSNDRKSSLDNDQFSLASSISRSSMNSRADDSLIFERLVQDPLTDAQPIPHSLPRHVSSEAFIPASLDTTTHMILNNDDPMEEMSNDDPANPSGVQLGFSSRRSSLANLEAALGHSSAASSRRPSIANLQSSFGMNSSYRLNSSSTNTLSRSQTNSSFSNLTQQFQNSNSSRRIPQSQPLSQSLSQNSQNSAPNIPPAGASNLPPLTTSLTSSSVNSLTNQRNPSSSPVLKNKSFCSYADIIAQDDHESKFQIRRPSISLSLNNQKLARTNSMSSASQICGCNSPRSPSNCAPSSFANNLRSNSTVSNILLPPNNANNNTSANISTSNNNKIIRGGSPSVKSDSNYIPLPSFALESDNRDYPINNVNNNNTPRRSGFSGRPIVNTGGHTMRLSNSNSSYNSNNYPGSNFNSPSHNLKKNELPSSNSRSIQNELISDDESIKSFKTANLPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKA9</accession>
    <name>A0A1E3NKA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Class E vacuolar protein-sorting machinery protein HSE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Class E vacuolar protein-sorting machinery protein hse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_72617</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STAM family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017672.1">
        <property type="nucleotide sequence ID" value="XM_019164250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKA9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKA9"/>
    <dbReference type="GeneID" id="30180937"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050670">
        <property type="entry name" value="STAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929:SF3">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="12">
        <location>
            <begin position="10"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="275"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="458"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="461"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="523" mass="58651" checksum="6827E533AB58CEF9" modified="2017-01-18" version="1">MTIGSLIDQITSPNLQDDDYSLLLDLVELVNRNPPSNVTEALRFLKVKLQSSNANTLLRSLTMLDFLAQNCGAMMKANIATSEFVNDSLLPIVDDSLIHISVKYAVVKEIYKLSKSFKDDESLSIMKHTFGNLRLKYPNLCNQAVDEIDGLNKFNSSSLGQNKTAAGRAGVPGGPAEEESEDLELKKAIELSLLDANPPSRQPLQQPIQQQQQPQQQHEQQQVRPALQVPNNAYANVTPLSPQQSNQPLSRTVSNQQVSMPQIITPEKKFEEDTSKPEKVVALYDLSSDDEDTLSFKKDDIITIVEEINADWLRGCLNGRAGIVPTNYVRKIPRTVDSDLENLISALNGSFDIEMTLSQLMDLNKKVKTSPMSSQQFESCLLSNSFPNKIQKIEQIKNNLKQILELHKLKLLELQSLQSNVDNSLKIYQSLITQMAPAPEDPTISKFIETYPSISSLSLNPQPSGQFQQTQQPQQPQQPQQPQQPHQLQFQPQNTQQQRMPPTSTGSANHYMQMQQPNSNMYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKF0</accession>
    <name>A0A1E3NKF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonyl/alanyl tRNA synthetase SAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_27104</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017712.1">
        <property type="nucleotide sequence ID" value="XM_019162477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKF0"/>
    <dbReference type="GeneID" id="30179164"/>
    <dbReference type="OrthoDB" id="288942at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002196">
        <property type="term" value="F:Ser-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043039">
        <property type="term" value="P:tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Threonyl/alanyl tRNA synthetase SAD" evidence="6">
        <location>
            <begin position="195"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="299" mass="31601" checksum="99952581518C5F3D" modified="2017-01-18" version="1" fragment="single">VGALACQRDSYLQTLRTTVLRCTPKPAAEAAAAASAAGPTKQLYEVELEDTVLFPEGGGQPADTGTIRAVAGSAAEAPPVRVLDVQRRELRAVHVVDGPLAEQAEVEVALDWRRRLDHMQQHTGQHLLSAVLDGLQLPTLSWSMGAPASYVEVPRRLSDAEVAAVGQAVNDEILRNTAVSVATPGGEPEGEKGALRVVSIGELDTNACCGTHLSSVGQVKAVALLGQTKGKGGASRLGFVAGDRVHQYAGQLHEVVRRVAGTLSSSVDELDDRAAALVKQCKRLQHREKALRRELAALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKT0</accession>
    <name>A0A1E3NKT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="3">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16561</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017842.1">
        <property type="nucleotide sequence ID" value="XM_019161257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKT0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKT0"/>
    <dbReference type="GeneID" id="30177944"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000245">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001123">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1510.10">
        <property type="entry name" value="Domain 2, N(10)-formyltetrahydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="654" mass="70316" checksum="C533CFCC7650AED4" modified="2017-01-18" version="1">MPQQFKRLKLDLVKPVPSDYEISRNQVPKHISDVASEAGIVAAELEPYGSYKAKVKLDVLDRLQDKKDGKYVLVAGITPTPLGEGKSTTTVGLAQAIGAVLGKTCFANVRQPSMGPTFGVKGGAAGGGYSQVIPMDEFNMHITGDIHAVGMANNLVAAALDTRMFHEATQTDVGLFKRLCPVKKDGTRDVPVGLTYRAKKLGLDLAKIDEWTPEQVSKYVRLDVDPKTITWKRVLDLNDRSLREVTINEAPTEKGLIRKTGFDITVASEVMAILALSTSLKDMRERFGKIVVASSKAGEPITCEDLGVAGAITAIMKDAIKPNLMQTLEGTPVFVHAGPFANISIGASSILADKMALKLAGTSASFTPEEVREKQGYVITEAGFDFTMGGERFLNIKCRTSGLKPDVIVIVATVRALKVHGGGAEVKAGAPLPFEYTSENVEMLAKGCANLQKHIQNAKQYGVDVIVAINKMSSDTDAEHDIIKKASLEAGATDAIVSNHWEEGGEGAKDLAEGVIKCANKEYPTQDVNESFHTLYSLEGSIEDKIETIAKVMYGAGSVSFSDVAKEKIKEYTAQGFDNLPICIAKTQYSLSHDAKLKGVPTGFEFPIKDIKASIGAGYLYALADKIMTIPGLATKCGFMNVEVNEKGEIEGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKV8</accession>
    <name>A0A1E3NKV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_11735</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BIG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017872.1">
        <property type="nucleotide sequence ID" value="XM_019159832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKV8"/>
    <dbReference type="GeneID" id="30176519"/>
    <dbReference type="OrthoDB" id="9985059at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037654">
        <property type="entry name" value="Big1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285:SF1">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133414" description="Protein BIG1" evidence="12">
        <location>
            <begin position="18"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="13">
        <location>
            <begin position="266"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="195"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="314" mass="35383" checksum="9AA7B293D59236A4" modified="2017-01-18" version="1">MRSPLLIVSALSGLSSAASFENTAPLLISSSALASREVSFRDIETIQKTSEKVKQITDSICLNSPEQSITYVRFHGLDESDITQEFVEQYSLSTVSDHVVYRTVEDAVDNIMGEECSGAIIASQSDDSWVEVMKDVNFQVKVFEIYKDSAKDDLKKILQFSDDSKVIIQGLPTFVNPSDAVLNQASLKLNHSLNRKKINNNSKREDIDYEEVERELQESFEEVNELLEDQLAEIYEKSDINAYTSGSQTNTKKHIVVDGSLFDKYGFFSTGIWMGTIVLLFLAWLLSIALGWLNSMQISYGAFEKPFDFEKKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL13</accession>
    <name>A0A1E3NL13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72846</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017924.1">
        <property type="nucleotide sequence ID" value="XM_019164309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL13"/>
    <dbReference type="STRING" id="763406.A0A1E3NL13"/>
    <dbReference type="GeneID" id="30180996"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="256" mass="29880" checksum="15A86417F198C131" modified="2017-01-18" version="1">MDFAHFCGWAYAIFWGISFYPTLYLNYTLKTSDSISLDYIVLNILGYFCYCISMYLQLYNADVRTQYQGYFEGKLPLLSNADLFYSFHGMALLLVLASQIFCGNTIWKFKNERKSFKIHTLSKMVLMLFLVFAIFSWKLGDPVFALLNFAMNLAYFKIIISLIKYIPQVLHNYRRKSMFGISRLQIIFDITGSFFSFAEFYLKSDIPIVEAIDANRGKVGITCVTSLFATIFLYQMYIYGTSRSEQRLKEKHYDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL30</accession>
    <name>A0A1E3NL30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_136436</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017909.1">
        <property type="nucleotide sequence ID" value="XM_019160091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL30"/>
    <dbReference type="STRING" id="763406.A0A1E3NL30"/>
    <dbReference type="GeneID" id="30176778"/>
    <dbReference type="OrthoDB" id="10260712at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="263" mass="27807" checksum="4961B189B66B4067" modified="2017-01-18" version="1">MVARTDKTLVVPLVAMGSVPQYAVQLVIDARREQLHKQPIDSLYVYPFAGREMSVAEVVGGRAASAGEMVSPWELYYSDEDGADADYMQMHSPVIPGCESLYVKQLVQLVRQRGYARVVLLDALDKGLWHGDSPVAPTPPGPESEVPPDVPVLRWGNRTLGGMKLAVDDETPAGQTDDADASAVWPGPLVRLVGEQVDAAGEDVAVEALAVPVYDGWNAPAARALLRAARVGGPLAAAGGETAAAEGPASDRDGLARLEGVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL36</accession>
    <name>A0A1E3NL36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16673</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017975.1">
        <property type="nucleotide sequence ID" value="XM_019161302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL36"/>
    <dbReference type="STRING" id="763406.A0A1E3NL36"/>
    <dbReference type="GeneID" id="30177989"/>
    <dbReference type="OrthoDB" id="951172at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="6">
        <location>
            <begin position="464"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="329"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="931" mass="103712" checksum="6179DA60EDD54E05" modified="2017-01-18" version="1">MSSSWTPDMASLQQLVHIFAGTLSPDTAIRQEATSALTQAKSIADFDNYLLHILLASEINESTRASAGIYLKNDLVKNFKFKKPDLQSHILELLPQGLLSNSTFVRRITSGIISAVFRTVGPQGWPSVVPDLISLISGAAGADAIDGASEAFLSICEDSARLLEQEVQSSAGVIVPMQLLVPRLVEIIANGSMAAKVRARALACLVHCINVGSHTLYVNIDSILSVVFALCQQNISDEKIQGQIAAIFVGILDKWPEKLYQHWSGVVSWCTHMVKEGDESVALQADEFLLSMSTSELPPDMILPYLEIIIPVLLEKMVYDETDVELMEAEDAKDDGSEDKDEDIKPQMAKSKEHKAIKKENEDGNKSNGRGVDATEESDEDDDNEEDNDDDFDNSYTWNLRKCSAATVDMLSTTFPKEVLQYSFPMIKESMNAQQWPIREASILILGAVSEAALQYAAEQLPELIPFLVDRIKDDQPRVRQITCWTLSRYAAWVCSEATTGGAFANYFKPTFQAIMDCALDKKKVVQESACSSLSDFIECSDPQLISQFVEPLLHHFQAYFANYRRKNMIVLYDTVQTFTEKVGEQLRYNPTYVDLLLPPLIQRWQELDDSDKDLWPLLECMSSVAASIGEAFAGCAEPVYERAVRILHKCIEEEKMANSDPTFVAPEKDFIVTSLDLVDGLIQGLTTHSGQLIAQNDSKTSFSLMELVLTCLDDNYYDVRQSAYALLGDCAIYLFDLLIKPYIQTLMVSIYQEITNLNDDAIASCNNAIWALGEIAIRADNNLIQQYLSNFFRVLEPLIHDFNADVTLLENTAVTIGRFGIFYPDQTAVVAQEVLIMWCELISGLEDNEEKASAVNGMCNIIMKHPEIANDLGNVRAIIDMIANYHEPSVELAEVFKVLLEGFKSGLGMEWDRIIGDSPACQSLRQRYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL95</accession>
    <name>A0A1E3NL95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">MGS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16715</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Also catalyzes the cyclization of FAICAR to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018017.1">
        <property type="nucleotide sequence ID" value="XM_019161316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL95"/>
    <dbReference type="STRING" id="763406.A0A1E3NL95"/>
    <dbReference type="GeneID" id="30178003"/>
    <dbReference type="OrthoDB" id="6017153at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.440:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005492">
        <property type="entry name" value="PRK07106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="593" mass="65355" checksum="C9EE5581C406CC28" modified="2017-01-18" version="1">MSEYKKTAILSVYDKTGLLDLAKGLVASKVRLLASGGTAKLLRESGFPVSDVETITHAPEMLGGRVKTLHPAVHGGILARNIESDEKDLETQHIEKVDFVVCNLYPFKETVAKINVTIPEAVEEIDIGGVTLLRAAAKNHSRVTILSDPRDYPVFLEELNKSGQNEISDSLRQTLALKAFEHTADYDTAISDFFRKKYSEGKAQLPLRYGANPHQKPAQAFVTEGELPFKVLSGSPGYINLLDALNSWPLVKELSASLNLPAAASFKHVSPAGAAVGLPLTDIEKKIYMVDTIENLSPLANAYARARGADRMSSFGDFIALSNIVDLPTAQIISKEVSDGVIAPGFEPEALELLKAKKKGKYCVLQIDPNYNPSSLEKRQVYGITLEQKRNDAIFNSSTFKEFVSKNTQLTEQAAIDLTVATIVLKYTQSNSVCYTKNGMVIGLGAGQQSRIHCTRLAGDKADNWWLRQHPRVLGFKWAKGVKRPEKSNAIDLYVSNQIPTDEPEKSEYESKFEVVPVPLTAKERRDWLDQLSDVALSSDAFFPFPDNVYRAVKSGVKYIAAPSGSVMDRTVFDAADAHNVVYVENPIRLFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLC3</accession>
    <name>A0A1E3NLC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_72953</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018042.1">
        <property type="nucleotide sequence ID" value="XM_019164341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLC3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLC3"/>
    <dbReference type="GeneID" id="30181028"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000017">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF73">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="19"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="164"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="24"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="334" mass="35040" checksum="65B333A759934C36" modified="2017-01-18" version="1">MLSRASVRQFSSSASSAYKVTVLGAAGGIGQPLSLLMKLNHQVSNLALYDLRLGKGVATDLSHIPTNSTVEGFGPENDGLAKALKDADVVLIPAGVPRKPGMTRDDLFNTNASIVRDLAKAAAASAPNAALLIISNPVNSTVPIVSEVLKEKGVYNPKKLFGVTTLDVLRSSRFLSEVVGTNPATETVTVVGGHSGVTIVPLLSQTKHKDLPKDKYDALVHRIQFGGDEVVKAKDGAGSATLSMAQAGARMAGSVLKGLAGETDITEPTYIDNPLFKSEGVDFFSTRVTLGPEGVKEVHPLGNLSTAEEEMIATAKETLKKNIKKGVDFVKANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLR3</accession>
    <name>A0A1E3NLR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Karyogamy protein KAR4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_58631</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017396.1">
        <property type="nucleotide sequence ID" value="XM_019163310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLR3"/>
    <dbReference type="GeneID" id="30179997"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045123">
        <property type="entry name" value="METTL14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107:SF0">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51592">
        <property type="entry name" value="SAM_MTA70L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="35898" checksum="16C43A090F6E13CF" modified="2017-01-18" version="1">MGSYSNSNSNSNANSNSKQNCYKNDYSNNYIHSGTLPFKYIRNSKQQVEGYPKLQRLYKLKEQQVEKYSTRPYGARVDTENLAKTLNSWVYDYDLQFDVIMIGALTENQLIYPLLTKLPIDKLCSKPGFLFIWASTKKISELSTLLNDSRTWAKRFRRSEELVFIPVDKNSVYYPKEWSIPEEQILEEVQWHCWMCITGTVRRSTDKDIIHCNVNTDLSMENSSTGNSAVPAQIYKVVENFSSSTRRLHIIPTSTGLKKHVKVRPGWVIVSPDIILDNFEPERYKKDIEAIGSKIPYNDEIEQLRPKTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLW9</accession>
    <name>A0A1E3NLW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018247.1">
        <property type="nucleotide sequence ID" value="XM_019163869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLW9"/>
    <dbReference type="GeneID" id="30180556"/>
    <dbReference type="OrthoDB" id="3997200at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="142" mass="15530" checksum="60972EC41302DB63" modified="2017-01-18" version="1">MFSRLAIQPVRAAARASAAGAAAAPSRRAPQLRSFASTAAALAKEDVEAARATRLSAFIEQIRSNEKVHSQLRSVQLIIASKIKTAGTDPSLMQQMQLLSDKEVRAEMLKLSDIMKEENVNISKEDVGFLMQCLKAQMDEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM04</accession>
    <name>A0A1E3NM04_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017731.1">
        <property type="nucleotide sequence ID" value="XM_019161219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM04"/>
    <dbReference type="STRING" id="763406.A0A1E3NM04"/>
    <dbReference type="GeneID" id="30177906"/>
    <dbReference type="OrthoDB" id="4138492at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133432" description="AMP-dependent synthetase/ligase domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="55861" checksum="EDC38513B44A5447" modified="2017-01-18" version="1">MWASLFIILGLSSLYILYHHFARELQEVDPILLNHQTSISETRKSSESAVYRSVDVPHGVTVTRGLSLRNGYKIRDGCLKDIWYLALNNHQTKIAFGDKSFTLGQVNSILHLIAQNLEQKNVQKVVVLGRLNESPELALVIMTCFFITDITVVLCNSFNDVNAIHNTPNGILVTNSSFIDKIPQKMFSEVIGINVNGGLSSNTSSTHFTQILDIGQDIESSTDFLYNPDTDFAKVNNHPYSFVSKGTETRFFQVNFVSAVAAKVMSIPSPLSWNEKDSLLLSYTTQSFSSTNILFGLCCGLISNIGSIQIIDPKNIGTLKDLSTYKPTVLCIDSHILKTLCSSTKKSFWQSFKLQRSEYLNSLGYFNSFGKIEKSLHLKMVYACQLSPVLSSFICNFCKSILGCRIVREVYTNYSIGPILKTNIYDFRIVQNRNLALLGVPANSVELKTVNPAEGESRGKLQVRGMSIGKGDHLVPDDEYWVDAGLEGSFARDGCFYSDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM07</accession>
    <name>A0A1E3NM07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_10198</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018287.1">
        <property type="nucleotide sequence ID" value="XM_019159647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM07"/>
    <dbReference type="STRING" id="763406.A0A1E3NM07"/>
    <dbReference type="GeneID" id="30176334"/>
    <dbReference type="OrthoDB" id="4356994at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="876"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="876"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="909"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="963"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1113" mass="125823" checksum="72EAB48FC915794C" modified="2017-01-18" version="1">MDRFNRGYPVPTAWSSNGDMSQAQQAQQSQSQNQHPPHHHHHHPQYLQQYHPQAQPPYRYYQYAQMSPPQSSVGMFQYDPAASGQLYAGNGISAGLHALSASSSGNSAGAGGTSNMENGSSPQISVGFGMHKQDLDLHKQQDASKSALDHHQQPYRQFVTTLLDSSTNKLVQITSQPPPDYVAAKKRRNRISISCMSCKRRKVKCDRERPICGSCQRHGYSVCVYAANDNDDSNKIRRHDNDSSNEDLPRFSSSTGLSSDNPGAGANGDGLVQDRFKFENDLILKPPQGLTNSGTAVSASSVDSSSPSHEFTNLLMVLEQLKLTHIQNDEIDQLKTRIEHLRFYLDDESISSSRTLKGLKLIPKIPLKPVLQTSSNVALDSFNLQLGASSITSYTEVDSYMGRLFKKSLPKIENDLNKWKSYFSVEIYRKALEKVFLSEKYSVNENLSLTDLKLAKFKLICKLLENYFVNYDNFATTMARSKEVLTISVPIVPRALIDQLLDDHFVKSESNKLEIVKIESDDDSSEILLVLAILRFGLPKSENVITTPDEIDYKTLLDYENITTDHKTDLLNSFLKLILHETDLSQKFNIPMLGTLVILFMISYTHRFNFKSGSTGVGLNYGVMAIYMAVNLGVYGGTKPADESKHQYLKFFKDSDFHNMWNLVMFIDTFSSFNSGIPQLISSGMDNMYVTPFVDSNCASICHFYRKAFTLANKSNTKKDDVSIIQYERFVIEFESFIVNKLVPTSVCIKKNDLVGVATSIRALNLLLFLYYNSYFSFIKTYENYKKNNNAITNPNYESMLQEFRELDKRLFERCIKLSIVSLIDLNIMLVSMFSEKDESFYEKYSFDLIQIFTRIIYTLTSCMCRMIVIRKREREQELDAEREHAEGKGSGKRKASASPDHYEDSGDNNSNNENNNNNNGTSNNINYAEATKNGKNSEHENNSEKNYSSEETQYSHSTGMRKGAGGKHGGSGSGANSSEQKSNSNENFNFLFDISRGQSEMLSNYFVASQYSSSLDGVSLELKFGPDIMSLSRSVDALSENPSAMIRLLIGFFFNTSQSLISQNFIYYTLYKYFVMAVKQLEETEGCTDTDDFDIDKFETHFSYVDCTWFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM70</accession>
    <name>A0A1E3NM70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15211</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018337.1">
        <property type="nucleotide sequence ID" value="XM_019160717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM70"/>
    <dbReference type="STRING" id="763406.A0A1E3NM70"/>
    <dbReference type="GeneID" id="30177404"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF16">
        <property type="entry name" value="HIGH GLUCOSE SENSOR RGT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="134"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="610"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="715"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="613"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="625"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="727"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="811" mass="89892" checksum="07502D2A81768169" modified="2017-01-18" version="1">MSLASRWDSRHDSLNTGHDVDLTDAIASSNTITAYTATNNSLMERPAFQSPSGTECTKKDKGKGTTNLPQGNSGSIITANSIDSEEKFREKIGDSILPVFPSLKSMKSNRKKQIINARTRKEKKIKSSFMGVYVGLLTAVGGFLYGYDTGVTNGLLEMDFVKQNFSKNGINFNSGEKAILTSIISLGTILGSLLSPYISDNYGRRFCLVWALITFFCIGIILQVSHASFALLLSGRFINGIAVGIISSVIPLFQAEVSPRWIRGSIISFYQWAITWGLLVSSAISQGTRHIDDDRCYRIPIGLQFVWCGILTIGLISLPESPRFYVMHDDIDGAIISLSRLRRLNIDDTELVEELIEIKASHDYETSDGATSYLDCFRSSPSRVHQVTRMLTGISLQTFQQCSGINFIFYYGVNFFVATGIQESYLMSFVTYAVNVAFTIPGILFVDKIGRRKLLIFGGIGMIISNYIIAIVGLFADTVVMNKVMLTFVCTFIACFASSWGPVVWVVTGELYSLSIRQKAVSLSASTNWIVNFVFAYSTPYLIDPGNHVAALGTKIFFMWGSFNVLGLIVTIFFVYETKGLMLEEIDELYRTCSSALKSGKYNKEIQSMSKMNQEKFESSDQQRPETGTLVNASGNDIGDTNNSIEMVPAAITRIRTDITYSDDSEAGLSQNRSNDSNGLTPMEYLSRWEQQNQRANILSDNIITRQDVLPLSDSDDDISNGSSQSSHDRNFLFDHGLYGDDPNRAFRQMEAEGAEDGGAEIGRGADHDEEQYRRNLREIVQEVSEQTGININLRGVDEDGENTGHTTDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM92</accession>
    <name>A0A1E3NM92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72752</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017821.1">
        <property type="nucleotide sequence ID" value="XM_019164287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM92"/>
    <dbReference type="STRING" id="763406.A0A1E3NM92"/>
    <dbReference type="GeneID" id="30180974"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005244">
        <property type="term" value="F:voltage-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097553">
        <property type="term" value="P:calcium ion transmembrane import into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056337">
        <property type="entry name" value="LHD_YVC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052971">
        <property type="entry name" value="TRP_calcium_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056336">
        <property type="entry name" value="YVC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859:SF5">
        <property type="entry name" value="ION TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859">
        <property type="entry name" value="NONSELECTIVE CATION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23190">
        <property type="entry name" value="LHD_TRPY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23317">
        <property type="entry name" value="YVC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="YVC1 N-terminal linker helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium channel YVC1-like C-terminal transmembrane" evidence="3">
        <location>
            <begin position="250"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="648" mass="74873" checksum="6289C2B3DB798510" modified="2017-01-18" version="1">MLTLPMWHDEEGNDYQAVSPRQALRVALNLKKLIDTVVPLAVPAQRIKSFDLNLPYDATLKAVYGAAGGEGEDRVSSARRYQACLVFCLLKVAGWYETLAEAELANNDIYGSRALFAQKLACIIIEHEKDDKYLFISMLCHRYSINLNDEDSDPENALELAADTHSMIISTSGYQRCIKWLWVGWIVQSRDDPSEYVLFNGKANTSFASHFDPDRIQTPLYQNLLEIFVAVLFLGLYTFVANIDAVPDSFNFAELLLYAFTLGFALDEVQRFYHFGSNYIQFSSAFNDTLYAIIFISYAFRVMAVYCLNSGNADAYNITSQRFLAVAAPFIWIRMFFFCDLYRFFGVMIVMINTMMKESLIFFFLLSVVIIGFLQAFVGLDQADGKRDLTYFIITNMLQTILSGPDFNSIERFAYPYGSILYYSYNFLVTVILLNILIALFSQAYSEVIENANDEYLHQYATRVLKYIRAPDAKIFFPPFNLIEIFLLDLPLYWWMDRKLFNNICNKIMLLLYSPYLCIIANYESKQARRINYNRKFNLSDDANEENREWLLTDGYEETMNDEANRHMIAQALQRQRDAESTEPEFAKHFPAWRKKVENLKPPINQASNAGVSLDSYQIIDHINKLTDKVETLIKQNTKMREELSSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMC1</accession>
    <name>A0A1E3NMC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcriptional regulatory protein RXT2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018388.1">
        <property type="nucleotide sequence ID" value="XM_019160736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMC1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMC1"/>
    <dbReference type="GeneID" id="30177423"/>
    <dbReference type="OrthoDB" id="2405722at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039602">
        <property type="entry name" value="Rxt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013904">
        <property type="entry name" value="RXT2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28232">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RXT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28232:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RXT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08595">
        <property type="entry name" value="RXT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional regulatory protein RXT2 N-terminal" evidence="2">
        <location>
            <begin position="45"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="332" mass="38905" checksum="FCDB342123674AFE" modified="2017-01-18" version="1">MVLTETEMSSLKEDILRFKERLEMEKNHQQYGASLSVAPVANPRSNRGSKMDPTSDTVNRNMISSCRYNYKLSSVDVYNNEEKRYESRLVYVNKEVDADLEEKIRQQVEEEFASDDECVPERDTLVDECEDSYVTRPNDIFSTIRLDQLLRPITKPSDVVTIKSIKSIYKERYLDKLSNETISIIEKEQENVNLLNQIMNIFLLDDPEHIQADNLGLAEYNHHLDLDKEEEDEASMSHDLQELHPENDPFFMPPVYESDSQFNGIDQEEIDETRQLIQIALQRNEEFVRSLLQIRLGFLHADNYREQVHSWCKEMHENEVAESQKSTNNNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMC6</accession>
    <name>A0A1E3NMC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_49852</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">TFIIA is a heterodimer composed of the large TOA1 and the small TOA2 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018385.1">
        <property type="nucleotide sequence ID" value="XM_019163142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMC6"/>
    <dbReference type="STRING" id="763406.A0A1E3NMC6"/>
    <dbReference type="GeneID" id="30179829"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000003">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="11">
        <location>
            <begin position="63"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="110" mass="12545" checksum="D19232C6DA3637E6" modified="2017-01-18" version="1">MNGPSYYEFYRRSTVGATLADALDTLIVDQRIQPQLAMKVLANFDRVTSDHLRSDVRAKMTFKGHLSTYRFCDDVWTFVIKDVNMKLDDGQMLDVGKFKIVACNSKKSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMF9</accession>
    <name>A0A1E3NMF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_136440</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 11">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017911.1">
        <property type="nucleotide sequence ID" value="XM_019160092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMF9"/>
    <dbReference type="GeneID" id="30176779"/>
    <dbReference type="OrthoDB" id="10056816at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10507">
        <property type="entry name" value="Zn-ribbon_RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034004">
        <property type="entry name" value="Zn_ribbon_RPA12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF14">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="12">
        <location>
            <begin position="80"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="123" mass="13459" checksum="BF9F15FB3F8DE3E9" modified="2017-01-18" version="1">MSAVGSLIFCTACGNLLDSISHQQHLTCSVCHSTYPSGDFSRLKVVTHTAPDAFPSSLKSKKSLVKTSLNRGEVEEGATIKEKCPQCGNDEMQYHTLQLRSADEGATVFYTCTKCGYKFRTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMM7</accession>
    <name>A0A1E3NMM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar protein sorting-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16477</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017746.1">
        <property type="nucleotide sequence ID" value="XM_019161225.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NMM7"/>
    <dbReference type="GeneID" id="30177912"/>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005798">
        <property type="term" value="C:Golgi-associated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120014">
        <property type="term" value="F:phospholipid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090083">
        <property type="term" value="P:regulation of inclusion body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056748">
        <property type="entry name" value="VPS13-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056747">
        <property type="entry name" value="VPS13-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009543">
        <property type="entry name" value="VPS13_VAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25037">
        <property type="entry name" value="VPS13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25033">
        <property type="entry name" value="VPS13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25036">
        <property type="entry name" value="VPS13_VAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="domain" description="VPS13-like middle region" evidence="6">
        <location>
            <begin position="1101"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 13 VPS13 adaptor binding" evidence="7">
        <location>
            <begin position="1946"/>
            <end position="2536"/>
        </location>
    </feature>
    <feature type="domain" description="Intermembrane lipid transfer protein VPS13-like C-terminal" evidence="8">
        <location>
            <begin position="3063"/>
            <end position="3169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="872"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1385"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="900"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1389"/>
            <end position="1407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3195" mass="360761" checksum="95817C94C5C63E73" modified="2017-01-18" version="1">MLESLVANILNRTLGAYVENFDPNQLNIGIWSGDVTLSNLKLKRESLDKLDLPLDLRCGHLGTLTMQIPWSNLKSKPVKILIQDCYLLVSAVQQTKFDKGSEQSRELRVKRQKLDELELLSQQQAEFSQLDEKDKLKNQSFTDSLISKIIDNLQITIKNIHIRYEDLNCFTKVPYALGFTLDELSAVSTDENWVKNFIENVSSASRKLLLLKSLTVYLDTSISSTASAAVFSDLDRDAMIDRLKAYIDQASANASGNIEYLLQPVSGTGHLTLNKLGPTDESPHYNVELFFEQFRLNLNRLQYCDILDSTTEMNYYQKTLKFRINAPHCAVSEDPYKWLQYSFQAIYNEIHERNYRKTWGYLKKRRDQRKQYIALWKKYLLDKNSLTLDEKKALTQLESECEFGDLKFYRSLAKMQFKKEHHALPAKAAAAKDANNNKGWLSSWWGSSTTAKDNEDKPTVLPAEGADLEITDEQINEFYDAIEFDESKVLSESFDVPRERIKVAVNCKLNTGSFIIRQNHSSNNLAEFISEGCHVEFLQRKDSYYIGFRLNKFSVEDGATDTLYKHIVSVKQLTSPPSTSPSPSPSSSNLNQLSLSDAVLSDNPFFQVSFEHNPLDESADSKLVAKLSSMTIFHNTRFLESLHKFFKPPKAHLDTIGTLMNAAESTIQDFTKQTKIGLQYAFEEHKTMNVKMDLQAPLIILPINPTKWDSPVGILDAGHISIVSDIISADKYQEIKNERKDTYTENDWKKLNTYMYDKYNLALQDAQILIGPDIKSTIEQLHTDGPKDALILDQLNMNFVIEVSIIPTFYDLPRMKIGGDVPRFVAMLSDYQYKIFMDLLSSSVPVFTANDDETDNSSITNQNDSDVQTFLTDSNAAPNMDSLEDSSFSETKSSDKDDQMANSTTSVSSPAMNQHAIEFNFTMDLIILSMKRCSNTTTFEAEKLVDLVGDKLKLNFYKTENVMHVDLLLADLSVNDYMENSNVDEFKKLVGSKANLETSTASSLDNLFVVSYTKSLRLANFKGEIIECYDQNVKLDISDFQVVITRKSLLTLLNYSLNTFTDPMAPEIPSDKLRHNDIENVNAPAHINVDINMKSINMILNDDGIKLATLVLERADVSVFLLPDSMRVSATLGGLNLIDNDTHRGRELISIQGDDLADLSYETYDPETNTLPYSSQFKFETRSMVVMFAEDSFARIYGFICQFQRMKYIYDSARDAALDQANNVEYPNKMKFDVLIRAPIFVFPKIVDPVNELFDTITTNLGEIHTFNEFKRKDDTYLNIIQAGLTNTKISSSFVVNGDNFQELEIIDKLDIIFNIDYYDGDKLPRPSMIVDGNLSGNDIKLSEWQTFSIIQILQSFPRIFKDNAVNDGNIEDIENDATNANMLIKRSKSPEKEPSELSKKSVDIEHASVSSESSTTSAVSKKPNSVSVDFAFNIPLLSLTLYNNTRQSIDVSDKKLSKFSLNDMAAKFQLFDNGDFKSDLHIKSFVVKDVRATTKNVFTDIIPEVDHTEYQFMGLVNSVNGITTIDVAIDSPKVLLVMDYLFALKSFGDFATHKPKTVVDQYLQDYNNSDENAENGKKIDEILDPQDSLDDDVVAGNQINYKVNIVDPSIILLANPENDDTEAVIFKISQLGLTIGELTQMNASGIGMFLRRMSSDDTSKLRILDDFSFDFMLDTRGSNKTSFLTTIDSNIDPLLLRLSLRDIYLAIEIFNKASSMFNEGSKGEIESQQHHVRHQGTSFTDEISKKISKYAPSVISSLSKASQQGRGQKSNEPLVIVKGERLKINIQGFRLVLIGDVHELPILDFDIKAFEINAKNWSTELEADTQVSSLINTFNYSLSQWEPLLEAWSFAVHIERSAQQKISVNFVSRETAELTVTSRSIATLSHFANLLSEKGELKPRGEDSPYRIINQTGYDLNIWIDDDSDSERQKTLLKDQETLPWSFEDWRQVRESLSINPNINYIGVEFIDSIYDPIRKLSLKAEGEEVLMLTPILKDQYHNRLACEIVLAEDKVKEVILKSTVSIKNFTATPIYVGVGNYDGEFVVDREIMVPTGANLALPIDYVYNGKLAVRPATTSEIFGWSTAKLQGNDSSITMSWESIRDHDLLLECPRIEQNGPADYYYFKAHAEYNEKESLNQLYPHMNIFISPPLVIENLLPFDIEWRLFQKGPRKWNDKLNQGEKCPIHIVDMNFSAVIKIQVLGSTYAVSAASIVNTPNNMTTVDKEINLKNDDGHRLSLKLYYSTDPKYGTKVSIYSPYLLVNRTGKDLFVMDSFNTLCSKSRQGVLTGDEKEFPDMFSFSIENDAFGFFRGNLERNTVQLRVGDSYPSEKFSIDKIGQSFEVRVGLKTRNFENDVGIYITEGQGVYNLTKVIIITPRYIIRNDLGIPIVVSLVGSSKTVNVGSNAASPIYDMPTYGDKQMMIGFKEGRGNMSAAFVINNVGEIYIRVKKLDSNAHMLLRVVISTEHASIFINILDAKNLWPYSIRNFSDNEFFIYQADPYIDKDGNKLSDAEFTPVFYKIPPKSVMPYAWDFPAAEVKELVVRCSGRERYIQLAEIGSLMPMRVKKEGGAFQIFDLNVIADGPIQALMISEYDPRTSMYQLKQNKSTSTTATTAMKDEFETNIKDEKYYTNIVFNFEGIGVSLVNTKNTYQELCYTTIRGIEFHYNESDIYQTASLKMKWVQIDNQLYSCIYPVILFPTIVPKSSYELNSHPVFSTSIARLKDNTHGVNYIKFATVLLQELSVQVDEDFLWAILDFSKIPGAAWNSDVKNELWDKNLQIPEPPTIRSTDDLYFEALHLQPLQFNISFVRTERVNVEDTMTTQNALSVAADVLTMAIGNINDAPVRLSALLLENIRTPVPYLIQNISEHYKQAFLYQLYKVLGSADLLGNPVGLFNNISSGVMDIFYEPYQGYIMTDRPQELGIGLAKGSLSFLKKSVFGVSDSVSRFTNSMAKGLTAASMDKDFQEKRRMTRQVGKPNHPVDGLSIGATSFINGISSGLSGLATAPMEGATREGASGFFKGLGKGLLGLPTKTATGVLDLANNISESIKNTTTVFDGEGIEKVRLPRFIPFDGAVVTFSEKDAQGQYWLKTCSGGIYSDDQYLAHVVLQGGDHACIISFNRILVMKVFDMTVDWQISLENILNITLEKTGIRIKTKNVSDPSRFIPLPDRADKRYLYSQIAIAVNEYNKHCIVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMN5</accession>
    <name>A0A1E3NMN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_40682</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018508.1">
        <property type="nucleotide sequence ID" value="XM_019163110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMN5"/>
    <dbReference type="STRING" id="763406.A0A1E3NMN5"/>
    <dbReference type="GeneID" id="30179797"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="239"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="355"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="397"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="59980" checksum="8E4B97DB18F95D62" modified="2017-01-18" version="1">MSVSVNEINYLIWRYLQESGLEVSAYALEDETTINQLDSVYSPHVPLGCLVDLVQKGILYSKMTDLVSAVKTDTIMQDDIINLNFNFFTALHEINTKEPTEDRKILSLEELNGSKQTRERSSEEEETKVKQEDGQQQEQAQDGEKSGFIQVVKGLMNYDSSYSVSFSPNNPELIAWSQRDNQSVIYSFNSNSETKLSLPLACKETLIVSWSPKGSSLITASENGELRLWSIEGTLLSTLAMHHWPIVSLRWSPSGRYLLSLDIRNVAVVWDITSSLVVAHLDKESWSHADKSFNDLISKDSANNESSSTGSPLYKNYGTDTCWLDDNKFVIPGPKYTLLVCQVTSDNSNSIIGVLTGHEESICCVKYDPKLRLLCSASEDGVIRIWKGNSTNSLQILRGHALAVSMLEWITISNRWFLLSTALDGTMRIWDFVQNETIDLVSVDQGQAILCASFYQGEDDTRKKIVTGDSSGSVCIWCIEGDSKSNIKIRQVGLYQHFEETNEEPQESGFVSEISWSSDGSKVAVSFSVGKSVVIGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMY2</accession>
    <name>A0A1E3NMY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Uricase</fullName>
            <ecNumber evidence="7 10">1.7.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Urate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71553</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>urate + O2 + H2O = 5-hydroxyisourate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="EC" id="1.7.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7 10">Belongs to the uricase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.3.3" evidence="7 10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018595.1">
        <property type="nucleotide sequence ID" value="XM_019163950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMY2"/>
    <dbReference type="GeneID" id="30180637"/>
    <dbReference type="OrthoDB" id="9992118at2759"/>
    <dbReference type="UniPathway" id="UPA00394">
        <property type="reaction ID" value="UER00650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004846">
        <property type="term" value="F:urate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.270.10:FF:000001">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002042">
        <property type="entry name" value="Uricase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019842">
        <property type="entry name" value="Uricase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03383">
        <property type="entry name" value="urate_oxi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874:SF1">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01014">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000241">
        <property type="entry name" value="Urate_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00093">
        <property type="entry name" value="URICASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00366">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4 7 10" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="302" mass="34104" checksum="B1337F3FB7202596" modified="2017-01-18" version="1">MAYLQDSSYGKDNVKFLKVKKDKYNPQKQEVLEATVRVLLRGNFDVSYTKADNSPIVPTDTVKNTVLVEARKTDVWPIERFAAHLAEHFVGKYAHVSGVDVHVIQHKWTKYEVNGKLHDHSFKKDGEDVKTTSLSYNRGGDFQLSSTLKDLTVLKSTGSGFVGYNKCDYTTLAPTTDRILSTDIDCTWNFDSKKIGSIKNVYKLADQGLFDQTMAKVLKITLDRFALENSPSVQATMYNMSADILDECPYVYNVSYSLPNKHYILFDLSWKGLDNDNVLFYPAPNPNGLIKSTVGRDIKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN10</accession>
    <name>A0A1E3NN10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72829</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017906.1">
        <property type="nucleotide sequence ID" value="XM_019164307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN10"/>
    <dbReference type="STRING" id="763406.A0A1E3NN10"/>
    <dbReference type="GeneID" id="30180994"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="36"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="342" mass="38406" checksum="CABB0DDE790E21E2" modified="2017-01-18" version="1">MSEQPWIEKYRPKSLSEVTSQSTTTGILQKSLHSANLPHMLFYGPPGTGKTSTILALAHELYGPNLIHSRVLELNASDERGISIVRDRIKNFARLAVSNPTPEDKVKYPCPPYKLIILDEADSMTSDAQSALRRTMENYANVTRFCLICNYVTKIIDPLASRCSKFRFRSLDEKMGIQRLRFIADKEKVNVSDHVIKEILQSCEGDLRRAVNLLQCVSTVLDDDSSDNRSGGINDVKIKELFGYLPDDLVDLSIQTLKSKNFIDIIAFTEEKIIKQGYSAAFTVIALHQKLLLSTEGISLELSSGQKNEIAKILWQTDKRISVGCNESVQVLDCMFKLCNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN41</accession>
    <name>A0A1E3NN41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glycoside hydrolase family 17 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71591</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018643.1">
        <property type="nucleotide sequence ID" value="XM_019163959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN41"/>
    <dbReference type="STRING" id="763406.A0A1E3NN41"/>
    <dbReference type="GeneID" id="30180646"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000111">
        <property type="entry name" value="Soluble cell wall protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF14">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133424" description="Glycoside hydrolase family 17 protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="36727" checksum="99255837CEA463C9" modified="2017-01-18" version="1">MLFQSVANAIALACMASSAVASPLGHLHHMHKRDDVVVQTTVVVHVGGNSPVATASTAASVDSVAAASASATATVTPAIISASAPASAPAPSASASSGSGSSGSFSGSSKGITYSPYNSDGTCKDLSTVKSDLAKLSDYSLIRLYGVDCNQVENVLQAKANGQQLFLGIFFIDQLSQGIQQMSSAISSYGSWSDVHTVSIGNELVNDGEASASQIGQYVSTAKSLLSSAGYSGPVVSVDTHVAILNNPELCQYSDYIAFNAHAYWDGSVTGGEAGTWLLQQMQRVASACGKNVMCVETGWPHQGGSNGVAVASTSEQSAAISSIQSTCGNDVVVFNAFDDMWKNPGSNGVEQFWGILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN65</accession>
    <name>A0A1E3NN65_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15483</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018667.1">
        <property type="nucleotide sequence ID" value="XM_019160844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN65"/>
    <dbReference type="STRING" id="763406.A0A1E3NN65"/>
    <dbReference type="GeneID" id="30177531"/>
    <dbReference type="OrthoDB" id="3995312at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="35945" checksum="E0C1D982FAA4BA70" modified="2017-01-18" version="1">MAKTKVTNHSNTLILTDATLLHPKNASPLAETLDALKEFVKTQSILHGVHSCLQTLHFKSFRRIIMIFSVPKIADLVYEYLTKLGIKIGFARYDNGISSCDLDPECDVCEASKADAQLAILQPKDAPESYPVEDRCPGAGVYGIKYNKAETFRKLEPPNPPIQMQSPPPSPYEGWIERPEEPPSSTTVGFHPKALGHLLYTYDVETANHFDQRERDSDDEMKMRRVFSSTIETAFSDPIKESAMDDLNIGEGLHDDDDDDDDDEASHVLNGSLFQKPTLIREVSDKAKFSKMKIPIVVVDTQEAENLRSHAAEIDSVKIGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNE3</accession>
    <name>A0A1E3NNE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pumilio homology domain family member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_32631</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PUF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018733.1">
        <property type="nucleotide sequence ID" value="XM_019162779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNE3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNE3"/>
    <dbReference type="GeneID" id="30179466"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014810">
        <property type="entry name" value="Fcf2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08698">
        <property type="entry name" value="Fcf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="449"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="469"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="505"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="541"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="577"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="613"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="649"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="732"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="193"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="335"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="399"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="403"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1009" mass="114353" checksum="AFBE2BD834B47843" modified="2017-01-18" version="1">MNSSWDNFHKTASVEESIVSNPWDTASNNGNKNIINLVAPNPISPIISQQQQQQQLQQHHQQQQQQQQQQQQQHDDVASIISSLNGVGLGNANARGLANQSGFLGGYKQPSNLNAFDEAVMSDSGSDALRDAISAISAPVATQPLNKSRFGPNMFSPASIAGVNVTNLKSANIASTNSTFLEKFASVTEKTRELESNNIGSRRSSMFNNEPKSPQQLFREPRKQSFSEKLESYISATASPNASRHLSFSSEVNNANAAAASEHNSIVNDPNVTANPSNFKNNWNPATAATFHPSYPMNELSFMQQQSIMMSSFPMGPPQNLPNNAYFPQMFPPFVPTGPFDDRGNRTDNKEDSERDSATPKAPEFFPIGINRSVSPYVIAPFNPYNMFGPNGSNNGVFPSMIPAPGSPIGTSPSPGRAGTPPNRSSRSPLSNSNKKNGKPAKFKNKHIVRSPILEEFRNNKDKKYTLDDIASHGYEFAKDQHGSRFIQQELETASEDSKEAFFKEIRTNSMDLMTDVFGNYVIQKYFEYGTSVQKKVLFELMRGSFNTLSLQMYGCRVVQRCLEGIPLTDQLEIMEELKPNILNLVKDQNGNHVIQKAIEQIPIENIPFVLDSLRHQIYHLSMHPYGCRVIQRLLECSGVEDQEFILNELKGFIYYLVQDQFGNYVIQHVIESGEKKDTDVILKVVLDNLIELSKHKFASNAVEKCIINLPVESRIEIYETMLKDNLTKDGELLEQSNLMLMMKDPFANYVVQKMVELIDMDRKRLLVLKIRQYLKLIQKNSHGKHLASIEKLNHIYDIFNRLKQTVEKEETKQETNEDGIKSYSGNKKQLTYEELKKKVSHLPSIELHLYPNGKKANNGKSSREIRRINDTVESVLPDLRKKTVTDRRLIKEQKSKEWFALPRVEMTDDVKRDLLIIKNRKYLDPKRFYRGEKWEIPENFQIGEVVAGIGEYGGRLKRKSRGQTIVDELLKDKNSSAWFDKTYGDIQTRKKNGGKKFLKDKIAKRKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNF4</accession>
    <name>A0A1E3NNF4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018291.1">
        <property type="nucleotide sequence ID" value="XM_019160696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNF4"/>
    <dbReference type="GeneID" id="30177383"/>
    <dbReference type="OrthoDB" id="4095076at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="457" mass="53210" checksum="BEE7A1D20EA93B51" modified="2017-01-18" version="1">MEKPLGLLALPSEILQKIFVQVPTELKRTCRTFVVMYNDLYRNLFVDRFGPHILRTIADYDYKYLVDYIRSFDYWRKDIRNIISEHYDLRLPYTDEDELEGEKVDPANKLNCQYIRDSWKLVYGIYVNRRIFVDYDDYTVNSYVNAPMSSVKIDKTLKLTPGLYNLSCALIIKTFAGMSSSVFKVLDGKTGDKLLEYQPASHFSELVPHNKFVLLDIGSFEVKKPKIVEQEMDESSSEESNGEEVVATMEEAVQSKLVDIRVVVEEAGVMVKSGYILCYIDVNAYQLKDCMIDSAGDLYCVNEKYWLAWWIENQPPLPENVVNVLLKKLYKSMEKSMKLIKPTTPHRRMGSFAESNLDARIKRADMSPVVVENEDETDGTDGTDGEVNLETYNREFYSKLNREGELIVREFKFRTMKDRRRYEEWIGQTGEENYSVKSGTVYTMEPLKWKLTTIMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNQ6</accession>
    <name>A0A1E3NNQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_15638</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018843.1">
        <property type="nucleotide sequence ID" value="XM_019160901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNQ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNQ6"/>
    <dbReference type="GeneID" id="30177588"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="14">
        <location>
            <begin position="404"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="181"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="256"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1003" mass="111440" checksum="4492AC13BABCE794" modified="2017-01-18" version="1">MAKKGKKNNQDYWDEEFEEDLPQAQELSAEPEPSETANSEGPEGPANTNAEDESSGVDFLSNLKKAKTKKDKKAKEAEEEKAKQAAAQQQKGPIIKTKKEKEREKKEREKQRKKQLAERKGASQVQKPTGTTPETTPAPESEASSKKDEPAAKEAPKAKKGKKVPGNIAALKKQLELKKQAEEEAQRLEEEEKLREEEEEQKKLEEEKAKEEAKKAKKEKEKARIEQLKKEGKYLTKKQKQEKLLQERRRAALLQAGNIQVAGLSKDNGSTPKSKATASRFKKKSSKDSKSNDSTSVQDSESPAVESEKGADEVEEEEALVDDWEQMALDDDDEEDEEEREEKEEEDFVAKDYDNSEPTDETEKKQVSPAPALKQPETTTNTVIPAKSALLPATTSISKDGKELRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPIDAIEQKTKVMEQYEKVSYEVPGLLMIDTPGHESFTNLRTRGSSLCNIAILVIDIMHGLEQQTLESIRLLRDRKAPFVVALNKIDRLYGWDATPNNSFRESLASQKSSVQSEFENRFTAIKVALAEQGLNSELYFKNPNISKYVSIVPTSAVTGEGVPDLLWLLLELTQKRMSKQLKYLTGVEATILEVKVIEGLGTTIDVILSNGVLHEGDRIVLCGMNGPIATNIRALLTPPPARELRIKSEYVHHKEVKAALGVKIAANDLEKAVAGSRLLVVKDGDDEDEMMDDVMEDLTGLLDSVDPTGRGVVVQASTLGSLEALLDFLKDMKIPVMSIGLGPVYKRDVMKATAMLDKAKEYAVMLCFDVKVDKEAEQYATEQGVKIFNADVIYHLFDEFTAYQEKLLEEKRKANEGSAIYPCVLKTIQIINKRNPMIIGVDVTEGMVKHGTPICVVKPDPLTGRKTPMLLGTVASLEVNHESKSSVRKGQTNAGVAMRLDPPSGQQPTWGRHVDEKDGLYSLITRKSIDTLKDPAFRNTVPREDWLLIKKLKPLLDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP99</accession>
    <name>A0A1E3NP99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019044.1">
        <property type="nucleotide sequence ID" value="XM_019160974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP99"/>
    <dbReference type="STRING" id="763406.A0A1E3NP99"/>
    <dbReference type="GeneID" id="30177661"/>
    <dbReference type="OrthoDB" id="3997768at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="662" mass="77615" checksum="77A24E113CD0CF79" modified="2017-01-18" version="1">MTSSTVLVTSSETKNDKDGANGSTLPHSKKENHTRHLYRYQATRMVSNLDEVRTFVDPLDESNVFDPATLFHYCNLILDQYPNENATLAVFQDDLNYGPQGEDVAIYFHPFEWKGQWHLAIGFTQYEKILFVNCSDYSLSEDSSRAEDYPSFSGKTSLRIQDFVVFFKQKLSINSLRYPRLYTDLFKPATGSNAVNLLTIIFAFCLDPVSLTRSFCDPQFSPTKFDISDFHPIIASIDGEFREELQHTNELKQKEKEKLVAIKKKESEHAKNVAKELKQKQKREREERERVEMEAEIKERQRMVQERIRLEKEDREKRAKERDQQQQQHDLHVAPIPVEHEVNGDVQTKIGDSLSTIQREKVKDEELETIVKRQKTRSRLGKSISAPIEINDLEDSDDASEEEKLPVKTQDVSRKETEETLRQRFQPVYALEFKKHILHSELFGDITKIDLMYSFIRQFSYDPLMNALLYRDGSADRSKKSMFELRKSLVVTYKRRMKQEFDESKFEKIMETDSLNQIIPLIFENGSIFLVNIKTSDKFNCIVKLICITNRLNRSEREIMKNYAFRRLIEMHVAKYNRLVLKIRTSIIAVKQSEFYKVAMASFCVLQRILWKNKFDLTYKPKVENLESYNVYFQKMMEGIETSDMLATELNLKRIVKYSLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPB3</accession>
    <name>A0A1E3NPB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15785</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019025.1">
        <property type="nucleotide sequence ID" value="XM_019160966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPB3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPB3"/>
    <dbReference type="GeneID" id="30177653"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="387"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="173"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="313"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="359"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="439" mass="48700" checksum="3E0E7A0C24EC8F8B" modified="2017-01-18" version="1">MAVDNLPPRNKSQRLIDRKQKQQLEEKFNQQNHNHHVKTIANPQISDICLGLNELTDSLHALPQDIISYFTLLKEIEAKCVYTVPHLQAYIKRFLTMRKDHPKRSLLLSRIRECIREIMPCLEEKMHVATIASDHVRKYVNKLDQAYDIIVDKEIPEIIRIGPLWEPCMKVSEPKTVHQQRSESRREALAAKKARNGGSFEDDYEGTAAEDSSGPMKKPQRSSKKETKTHSSTTSTANVNSKKRKTAEEDYNYNKQTGTNGNGAHTQNGTSSNMSVHSTAKKSKSSKVKKETKRENDHSIQSSRNGSSSVNHAKSANGGTNGAANSHGGGNANSTNSNISSSRSAGGGSSKARSGNHSDSGHASRARKESQHKVTTATSPANYEGEPVYCYCQQVSYGEMVGCDGAHCEKEWFHLPCTGLKELPKGEWYCDECKAKMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPD4</accession>
    <name>A0A1E3NPD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018516.1">
        <property type="nucleotide sequence ID" value="XM_019160787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPD4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPD4"/>
    <dbReference type="GeneID" id="30177474"/>
    <dbReference type="OrthoDB" id="2501249at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070124">
        <property type="term" value="P:mitochondrial translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052837">
        <property type="entry name" value="Mitoribosomal_bS21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP21, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19217" checksum="D18998DDFE300527" modified="2017-01-18" version="1">MFTRSVYPAVQAARFAARGNSTLSNTATTVKMVSSVKSGQNPSQTKTNNIISEALGSPSTDYSSSFFSKRSFAPLSSTEPDPYDYATRFILSDKLAGRSVSVVNRDLDRAVYQFDSLVRSNRLREVSYDQRFYTKPNKRRLAKRVANRKRVFESGIAKLFEVVKDAVRKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPF1</accession>
    <name>A0A1E3NPF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CTLH/CRA C-terminal to LisH motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_112974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018536.1">
        <property type="nucleotide sequence ID" value="XM_019159777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPF1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPF1"/>
    <dbReference type="GeneID" id="30176464"/>
    <dbReference type="OrthoDB" id="2415936at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH/CRA C-terminal to LisH motif" evidence="2">
        <location>
            <begin position="186"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="448" mass="51623" checksum="F83625FB550E8EA8" modified="2017-01-18" version="1">MMNQILGELLNCENERSERDEGAESNNAIQNDNDNGDSSKSVHNGKNTHRRVSSTPIWPTELPDVFLFPDTFFQLQKDLNYGVLEYLIRMGYAGAADTFSKELQYEDKNIHNNVKQDDDFKGQDDKVVEDEDSNEFTEQSFDLQKEKMSQLFKTENLSAESNETLEKSKLSIKQQRATIGMESVYERKAMMIMLMNGQVKETIEFISRKWPHFFNEFRILKLRLLHLQAVEIIREFFDSDVSDFTGEELEEKEETFFNEFVEFIKKNLSDENILKSSRFIKDMELTMALISYGTFKEKESKVSFTERLPPELKKLVDISLRETIAVRVNKALLTYVDGVSFSDELDGGFDFSAASDSVASHEEEEDENEEKLCQEGSGYGVGDDDPNDPLLRAGYGDFRVLQRFSNGDSKLLQLVKLFVWSYKLNSNSNSRLSNRQKLEKMAEITLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPH0</accession>
    <name>A0A1E3NPH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin-D1-binding protein 1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019117.1">
        <property type="nucleotide sequence ID" value="XM_019164070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPH0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPH0"/>
    <dbReference type="GeneID" id="30180757"/>
    <dbReference type="OrthoDB" id="4088536at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026907">
        <property type="entry name" value="GCIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049317">
        <property type="entry name" value="GCIP-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492">
        <property type="entry name" value="CYCLIN D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492:SF1">
        <property type="entry name" value="CYCLIN-D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13324">
        <property type="entry name" value="GCIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like N-terminal" evidence="1">
        <location>
            <begin position="57"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="353" mass="38800" checksum="E804D35F276C3D6A" modified="2017-01-18" version="1">MTTVNMANTPDDLKEHLSKVEESLSHLRTSCQESVTTHSPTISNTLEVANVAQPLNEVPKLSSLLSAHATKLGLVFKPPIAITTYKACRTEVDSLLKYAALLVSLLNQIQEEKEKYSSVFANELSSDGLSVLEACVALISGLKELVSFGEATETVLTDQRLVGVGFIWEACEKLTKTCKNGSSGVLRSKLKQTNRLVVDALNELNEWLENPIVGGGFDFDEEDIFGLSNDIGSDGEEEKAEDKVIAFGKLWSTRIQLVKLLVSLLDKSVPASKYNPKFSKGLDALNAKCLKVSEHVDDVVACTVYDANVESGELASKSLAKEINQIVDLTRKINNNDEKKCKWLDSWKTKFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPY1</accession>
    <name>A0A1E3NPY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15974</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019270.1">
        <property type="nucleotide sequence ID" value="XM_019161041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPY1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPY1"/>
    <dbReference type="GeneID" id="30177728"/>
    <dbReference type="OrthoDB" id="6512771at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF12">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="32"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="R3H" evidence="13">
        <location>
            <begin position="706"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="968" mass="109569" checksum="6FC021F292A0FB2A" modified="2017-01-18" version="1">MLEPLNEGGAERGADDLRTSILKEIGASTYTCLVCTDYITPSSKVWSCDHCHRAFDLDCIKDWALRGSSTQDDKSWRCPSCNRSHSKIPKLYTCWCGKSINPTLNVLEPHSCGNTCGSPLKTCIHECSLQCHPGPHMERCTALGPVLKCRCGRHSKQLPCVLTPYDRGWSCDEVCNDILPCGIHRCKQKCHSGLCGECEEEISVRCYCGKASETIVCHERLPIKSEENGVAWIGNFECNDPCGEFFDCGVHKCEQNCHPVNKDSHKCPNSPLNLIYCPCGKTKIDSLLDSPRKSCLDPIPTCESVCGKRLPCGHKCYWNCHDGPCSPCYRTVDAECRCGFTSFSIACALITQGYTPTCTFKCNAKFNCKRHYCTRVCCEFRQVAFDRSKLIKKHLRKNIITSAIAESVEFEDVHTCQKECNQLLSCGKHRCKATCHPGSCSPCLESSSDDLVCNCGRTVVPAPVRCGTTLPICPFQCARQTTCGHRPEPHHCHEDDVSCPKCTMLVTKRCQCEKQNLVTNVMCYQDRVSCFRLCGKKLPCGEHECKKVCHKPGDCQKKCTEKCLKTRKCGHKCQQTCHFGKLCNEALPCQEHVTVTCNCGRRKQPLLCYTVANMIKESKSSHNAEVNGSSIDDESFTPHIACDEMCEKERRNRLLFDALGLNPARAKDTEVMLRMRTVESIYTPFTLNIYAKQAAWCKGIEEIFSQLLAHTMESSFRSLSNSEIKQSHHFRPMREVQRRFVHELAESWGLFSEAHDPEPHRSVFVKLVKSSKIPDIKLQEAHEIYQKYKALEKKKSLERVSRQEEKEKHASDSPEHIYFNGIIIKDVFFGVTVETIDASFFNIWNQTENESRKFPLVKNGRVEFICENMYVFYGDSCLEEEKPALEKQIEKLRPLFDKRVKDSNLALKCILAKVNIEEGTVVEMMDESNHRDEEATGKAQIHDNLVDVATETIDDVKVEAVTVPSEWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPZ0</accession>
    <name>A0A1E3NPZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_23573</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP domain-containing ribonucleoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018726.1">
        <property type="nucleotide sequence ID" value="XM_019162314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPZ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPZ0"/>
    <dbReference type="GeneID" id="30179001"/>
    <dbReference type="OrthoDB" id="445357at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052240">
        <property type="entry name" value="SAP_domain_ribonucleoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040746">
        <property type="entry name" value="THO1_MOS11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551:SF1">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18592">
        <property type="entry name" value="Tho1_MOS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="4">
        <location>
            <begin position="4"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="40"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="153" mass="16281" checksum="DF6CF2AC39330C45" modified="2017-01-18" version="1" fragment="single">MTKYSGLLKAKLQELLTARGLPVDGTKEVLIERLEESDKIAAELDQDDDDGEDTSAAGAGPTAAASAEVVAEPAAEEKKAEDAAPPEEKEEEKKPEVTPETLKAAAVEHLTKKIARAQKFGEDQQATTLQADLARIEKFGLALDSAIARELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ43</accession>
    <name>A0A1E3NQ43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication complex GINS protein SLD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15998</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GINS4/SLD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019298.1">
        <property type="nucleotide sequence ID" value="XM_019161048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ43"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ43"/>
    <dbReference type="GeneID" id="30177735"/>
    <dbReference type="OrthoDB" id="338231at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11711">
        <property type="entry name" value="GINS_A_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21692">
        <property type="entry name" value="GINS_B_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008591">
        <property type="entry name" value="GINS_Sld5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031633">
        <property type="entry name" value="SLD5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038749">
        <property type="entry name" value="Sld5_GINS_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206">
        <property type="entry name" value="SLD5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16922">
        <property type="entry name" value="SLD5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="8">
        <location>
            <begin position="109"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein SLD5 C-terminal" evidence="9">
        <location>
            <begin position="201"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="251" mass="29627" checksum="FF66472F86FEF6E5" modified="2017-01-18" version="1">MNGNALSDSNEGRDDRRRNDQQIVKGIIDNFENEFYEGLNTSRGAGNERTSEREKDEDMRSLRQAWIKEKMVPDILPYEEGLIERILERIRAQLEFIEMNSVELQTHEKDIKLMLVIVESELERVQFLIRSYVRLRLQKIDQYALHIQENDSQREKLNRDEVTYMEGHLTMLYELFEDQFLKKTRSRNSNIGEDQDMIDEPDLNRHVFLQCTQPSRAPIDSEELVLSPGEVYVMRYRSVAKLLSRGEAVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ62</accession>
    <name>A0A1E3NQ62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_16017</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019318.1">
        <property type="nucleotide sequence ID" value="XM_019161059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ62"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ62"/>
    <dbReference type="GeneID" id="30177746"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045009">
        <property type="term" value="C:chitosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054295">
        <property type="entry name" value="CHS4-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22997">
        <property type="entry name" value="CHS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1088"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1122"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1148"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase 4-like" evidence="12">
        <location>
            <begin position="438"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1224"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1224"/>
            <end position="1235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1277" mass="144843" checksum="07C55EF02F41178A" modified="2017-01-18" version="1">MAHPYKDMLEQSGESSGSTFDSVDTESGNFTRKRSLVRRDRARPNSYQTSFRNSRYSKNTISNSNSARLSAYQDNTNSSNIYYNNNSNNFGSDNYNNDRFGAGNEDIPMQNMSQFSSSNHMHHRNSPEAYAYHHDDIPENPNPFVLGEEDEGNDSKAGKDTTIERVTEYETPANPTPNQPREIFHLNDEYDDHDPHGQPLPNEYNVTGLDHDNDEKTPLSNENYYTAIPPKRMELEEDKHGFPYWKLYCYVLTFWAPGPLMSLFGLKTKDRQYAWREKMGLISIILVIGCIIGFLTFGFTRATCTNLTARLKPQDLSSSYMVINGRAYDLSSSSHPAAEGIDADTNVLYPPINAGGKDGSLLFQNVNGNCKGLIKPKQDSTIPFQGEEMAWYMPCKIMELDGSSTPNFTFDHYNGYACHTSSYARKTFYGLKVEGDLYYTWEAIANSTRDLIVYNGNVIDMDLLNWLFADELTYPQLFDDIKNDKTMKGYDISLLLTEPHQRQAADCLIEIAKIGVVDVSTVGCIASTIVLYVSLVFVLSIVIVQFVVACYFKWFVAPHQGVSYSSIKEMNERNNQIDNWVDDPYSSAPLADVPKKRRADYHSKFNKTFRLSWGGDINDFIGDGDKDIVIEREKRDDYKPQYITMTTEAYMMANAGKKKGRSMSRSSMLSTSNLTLNPFKKNDPFEDEEIETLDPDIISTDVIPQPPVNWEPFGYPLIHTMCLVTCYSEDEDGIRTTIDSIATTDYPNSHKLIVVICDGLITGAGNDKSTPEICLDMMTDLVVPKDQVQPFSYVSVAHGSKRHNMAKVYAGFYKYNDSTVPIEKQQKIPVLLVVKCGTPEEMTSAKPGNRGKRDSQIILMSFLQSVTFNERMTPLQYEMLKAIWQITGLMATMYETVLMVDADTLVYPDSLTHMAAEFVKDPEIMGLCGETKISNKAQSWVTAIQVFEYYISHHQSKAFESVFGSVTCLPGCFCMYRIKSPKSTNVWVPILANSDIVEKYSDNVLDSLHKKNLLLLGEDRYLTSLMLRAFPRRKQVFVPKAACKTVVPDKFQVLLSQRRRWINSTVHNLMELALVKDLCGAFCLSMQFIIIIQLIGTLVLPASITFTLYIIIIAIVSKPTPVMSLVLMAIIFGLPALLIIVTVSNLMYVGWMIIYLFSLPIWNFVLPTYAYWKFDDFSWGDTRKTTGGDKGSHGDREGEFDGSQIKQMTWREFELQRKDATTRYPSDRYVDDKNDPSNIASVYNPGNYDYEPDTEYVDMSRNFSSKPSTAEDMPNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQC6</accession>
    <name>A0A1E3NQC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019413.1">
        <property type="nucleotide sequence ID" value="XM_019164138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQC6"/>
    <dbReference type="STRING" id="763406.A0A1E3NQC6"/>
    <dbReference type="GeneID" id="30180825"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000907">
        <property type="term" value="F:sulfonate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044273">
        <property type="term" value="P:sulfur compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="85"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="402" mass="46571" checksum="4679231A8F9BCCC5" modified="2017-01-18" version="1">MSLLYRKYRDSSIHPQKGVDEKINGVLTVSKDDQEHLKFPKWAPAWDSKDNHGFQNPRPFKFIDRGFYGDPSFESLRSIEGVVFKKISPNLGLEVNGIQLSSLSDKQKDDLALLVEQRGVVAFRNQDFKHQSFEEIKEWGSYFGPLHVHPTSGAPLDQPEFHLTFRRGSKKEHERFFNDRLNNVGWHTDVSYENQPPGITLFAMLQTGESGGDTQFIDTIEVYNRLSPLLKDKLDGLEVIHTSKDQAAAAREEGGIERKEPIDSIHPLVRYHPVLKKKSIFAHRGFSRRILGLKPEESDNLLLFLFKHTNSCLDAHVRLSWDENTVVIWDNRRVLHTRTLDWDSEDIRHAFRLTTLAERPVGSQKEYDNWSPELEEENLKVVDHYINLTPQEYFDEVLSKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQD2</accession>
    <name>A0A1E3NQD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CASTOR ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_51566</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019404.1">
        <property type="nucleotide sequence ID" value="XM_019163156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQD2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQD2"/>
    <dbReference type="GeneID" id="30179843"/>
    <dbReference type="OrthoDB" id="58529at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CASTOR ACT" evidence="5">
        <location>
            <begin position="131"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="749" mass="85458" checksum="F9614547D29DF2DD" modified="2017-01-18" version="1">MIDSTETQLFKSKISILTIPRENFWIFKSGTLEILYRLVDAYENRANSQYSDNDQGYQLSNSAHMINEEESESLDNGDINSEIEDDGLFFHLAFTREEVTIMCSTKLMKRYLSKAMKMDPESTLISDEFFIMQVFSDGSNIGKKILELTQPLSLNNISLFFISNYFSDLVLVPIKDKVKALKILEETQNGLDETRANSYSNDLELKTFELFENNNIKPKLGTDIQLLLTGARSGDYAEVLKQTAESVSRLNTENLKSGGKSFPEYFAITRTPTEEIGLLLPYDNTELNKLKFSKNNIMGSLQDYYYPIFIDLKKLPLDLKGIVAGMASKLLRMDIDEMSYLSLGKSGKARRQHRSPSCLLRPQPISSKFESYVPKYYDVGVNITDSMFSGSYHGRQHHRNDTLDVLKKAARHHVVDILLTGSSLDESKRTIEIINSLKQQVTNDKTLKLPNLYSTVGVHPCTVLEFESAGSVEQHLQDLENLVSEGIEADVVRAFGEIGLDYDRLHYTPMEKQLVYFEKQLRLAAKFDLPLFLHMRSACDDFIRTLKPFLDNNLLKNKNLLVHSFTGDKMQLEQLLQLEKSNPHGYKVFISINGAGLRDTSTLEVLPLIPLDRLMIETDSPWCEIKKTHPSWKYLSTSPNSFYPCDYENYETVMQSPEVQGLMKNSAKNKKNSNNTNLTLFDFLPIPTVKSDKFDKFKYSHIFEDSPLIKSRNEPCLIGLVALVISKVRTEDPQKIIDSAYRNSKQVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQF7</accession>
    <name>A0A1E3NQF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cell division control protein 50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_32652</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018886.1">
        <property type="nucleotide sequence ID" value="XM_019162781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQF7"/>
    <dbReference type="GeneID" id="30179468"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015247">
        <property type="term" value="F:aminophospholipid flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140345">
        <property type="term" value="F:phosphatidylcholine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044088">
        <property type="term" value="P:regulation of vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF20">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE ACCESSORY SUBUNIT LEM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="44653" checksum="5DA6CFC8EB6CFCE6" modified="2017-01-18" version="1">MEDDDEADSELEKKPKGKSRRPKEDDFTQQRLKAIHPILTASAVIPMFVCLAIIFIPIGASMLHASNNVQDFTINYAECSTRANSDTWTDVPLELYTHHFKSKVSTTPKWKLARNDSFVWDGFDEERNICQLQFEIPNNLKGPLFLFYKLTGFHANHRQYVKSFSEDQLNGKDASVSVIKDTVGQNCQPLSVDDAGKIIYPCGLIANSMFNDTFESTLFAVNDTDASNYAMTTDGIAWSTNKNRFKKTSYNLSSIVPPPNWSKLYPNGYNETNLPDIGHWPQFQNWMSPAALSDFSNMALRNDHDTLTRGLYQLSVGLHFPTTEYHGGKYVYLSTASSIGGKNAFLGISWIVGGLICLALALVLAFGVLVKGRRPGDTNLLSWNRHHDGHDDHTDHTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQF8</accession>
    <name>A0A1E3NQF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_30338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019457.1">
        <property type="nucleotide sequence ID" value="XM_019162636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQF8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQF8"/>
    <dbReference type="GeneID" id="30179323"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF52">
        <property type="entry name" value="ADIPONECTIN RECEPTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="296" mass="33402" checksum="20DCC4D70D966503" modified="2017-01-18" version="1">MGGSLKYRHNHEKPHRSLVSYHDLPHWQKDNDFIIYGYVSETNNLWHCFDTLFFFNNESLNIFTHLLPGTNFISYVPPWLVHLPAYTNTDASDYGIFALFFIGFMVCLSCSATFHMLKVHSHVVASMGSRLDYAGILLLIASSLIGIVHYSFIDQPALRSIFVSITSVIGVVSLLVTWHPDFRTPKWRPIRTSTFVTFAFSGLIPICYGFLIMDWKVAADRAGLKYVGYEALSYLAGATMYAFRIPERLSPGTFDMVGNSHQIFHCLVVLGAYCHLRALVHAYIVAKSTTLGGQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQK2</accession>
    <name>A0A1E3NQK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bromodomain associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15711</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018936.1">
        <property type="nucleotide sequence ID" value="XM_019160928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQK2"/>
    <dbReference type="GeneID" id="30177615"/>
    <dbReference type="OrthoDB" id="5402929at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00076">
        <property type="entry name" value="HFD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromodomain associated" evidence="7">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="416" mass="47497" checksum="59C791865F112D56" modified="2017-01-18" version="1">MNKSRFYFHLFEVTIVQMLKAHGFDKANNQHVLEVFTDIAVRYFNLLVRTVIKFMELRDDCHPNIKDITLAFLELKVISPHKELDKYDIDPLTNTGIENFEKWFNDDMNTRLREVARPTRELLETNKKAKLQLLNVSSKMDNLTKALDEQAKQAQLQNPTMPYLPPPSVSSTKQTPTPYNMYTGIQTFPLADPQLGSSFNTQENQQSELLDGDHNESENLMDGYEIPPSAVDEDWIQYLIRDQIATHLVSNGLHQKNHSTQSANGKPSDNHHLKPALFKDTVLNEYIPKDLRKFIETVPKARNDFLIAGPMPEKLLHTFPYYKSDDESDYLTGDENDSGSESDGSGEDGDEEVEENTTHGDKNNTTNKLDADKHASSGLAAYDYYEHHKLYEDEMEDLDLYGQGDSTASHDLNLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQW2</accession>
    <name>A0A1E3NQW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70122</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019603.1">
        <property type="nucleotide sequence ID" value="XM_019163563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQW2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQW2"/>
    <dbReference type="GeneID" id="30180250"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="91"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="713" mass="79378" checksum="21785E6D5C2FDAF3" modified="2017-01-18" version="1">MVLMNVPVGEAKPGETAPRRNYKFKNAAVTKPKGYSCSTVYEFFNEAVSKHGKNRPCQGWRDLIDIHVETKKVTKIIDGEKVSVDKKWFFYEKSDYQYISFGELQKTINYYGKGLLKIGLSPSAENKLHLFASTSPYWMRTFLAAQTQSIPVVTAYDSLGEEGLTHSFVQTGSTTIFTDNDLLVKLINPLKKATEVKYIIHSENLDPNDKRSDGSLYKNAVKAIEEIKKIRPEIKFYSLNEVIKLGQDNDATIDVHPPKPEDLSCIMYTSGSTGVPKGVVLSHENVVAAIGGVGGNIPDTLIGTRDRVIAFLPLAHIFELVFEMLVFYWGGIAGYANVKTLTDASCRNCEGDMKCFKPTIMVGVAAVWEAVKKGIISQIEKQPSTTQKVFWAAYNAKIASKKYYIPFVPSLIDTLIFKKIKQATGGQLRLVLNGGSPINSETQRFITNTIAKMLIGYGLTETCANTCVLTPELYEYDVAGSLVASVTVKLVDVPEAGYYAKNDQGEVLISGLPVMKEYYKNPEETKKAFEYEKGWFSTGDIGEWTSSGALKIIDRRKNLVKTQNGEYIALEKLESIYRSNKYVLNICCYADENKVKPVGIVLPNEAQVKELIFDLGLDKKAKKADDVEIAHYLHDKKLRRELTKSILETGKTGGLTGIELILGIVLVDEEWTPESGFVTSAQKLQRKKILASAKEEVDKLYDDNEANTTSCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR13</accession>
    <name>A0A1E3NR13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit C N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019635.1">
        <property type="nucleotide sequence ID" value="XM_019160213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR13"/>
    <dbReference type="STRING" id="763406.A0A1E3NR13"/>
    <dbReference type="GeneID" id="30176900"/>
    <dbReference type="OrthoDB" id="29647at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit C N-terminal" evidence="6">
        <location>
            <begin position="112"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit C N-terminal" evidence="6">
        <location>
            <begin position="268"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="216"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="881" mass="99590" checksum="D048A41CE5601CB4" modified="2017-01-18" version="1">MSTFFYSSYDSESSSDEDTLYYSDDERHLEQRQTSGKSLLITGESDDSGEETMSESEDEAPANKSAFLKKAGSDEEDDEESSDDEWNNSDSDSDSDAEAPKGRSYFLKKDFLKGNNSDSDSDDEDEKKVVRSAKDKYLDEIADLTDTIENLSMVEEWVRIASEFDKLFKLAYRHNQYHLSIPRSYIKAISVLEDAITNESSSNSNEEGKKLNASESKSLNILKQKIKKEVKAYREEVDLYKKDPEAYEAIKDEAQFLEVEASDAEKKELVVSIENLFTIVQSIIESRGKKGIDQRENLKTLNELLQIAQSTYEKIVLLNLIISSRFELHTKEQFMPTAEWNIIFEDISKLLDVLDADESYIITEVAPPNDDITTPPPANSEGKHLIVGSVTSFVERLSDELTSHLLYLDPNSSEYISLLKDDSKFYGLVVRAQTYLTRIIPTKSYGEIEGDQLCRIIIKRLESIYYKPVQLIVLAELNAWNLLNTEDNSSIAKIISSEATDALNISQVNSLLDSLCSHLYQYSTGAASPVYRKRAALMQAYYYATSNQFFRARDILHLTHVQTTIHTSEPGVQVLFNRAIVQLGLAAFRSGLLEEAQTILNEVVTSPHTRELLGQGRIYFSNSNSNSADNGSSEKGKYVPFHMHVNIELVDAVYYISSLLFEVPLMALQAYQGSTPGAVAYANGEAQTQAQNAKKNFSRSFKRILEYTERQYFQGPAEETRDTVFEAYNHLVKFNWKTASKILCELRVWNMMPGVSVSNSQGKTGLESLNDMLVESCKIQALKTWTYVNSGTIKNYSVRKLAARFEIDESLVCNTIGGLIYREEIRGFLKFNDDGSKTVVFEKKEDGMGEIVRGLTDRVNSILERNEKLSLGGYQIMLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR53</accession>
    <name>A0A1E3NR53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Malate synthase</fullName>
            <ecNumber evidence="3 10">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_14105</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the malate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="3 10"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019664.1">
        <property type="nucleotide sequence ID" value="XM_019160224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR53"/>
    <dbReference type="STRING" id="763406.A0A1E3NR53"/>
    <dbReference type="GeneID" id="30176911"/>
    <dbReference type="OrthoDB" id="186072at2759"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1220.12:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.360:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="12">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="11">
        <location>
            <begin position="176"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="13">
        <location>
            <begin position="426"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="556" mass="62489" checksum="9270D9FD79EDEB22" modified="2017-01-18" version="1">MPARLFVTSLDDVQVLGEVSDKPLLSEATPKDILTVDALKFVVMLHRAFNEKRKELLVNRQKVQEELDAGGSLHFLKETEYIRNDPNWRCAPNHPKLMSRKVEITGPPDAKMIVNAFNTDVHTYMTDFEDSCAPTWSNMLYGQVNLYDGIRDKIDFTNEQTGKRYKINREGRRVPVMIVRPRGWHMVDSHITVAGEPISASILDFGLFFFHNAKYLISQGLGPFFYLPKMEHWLEANLWNDIFCVAQDSLEIPRGTIKATVLIETLPISFQLDEVLYALKDHSAGLNCGRWDYMFSTIKRLRNQKEHILPDRQQVTMSVPFMTNYVKQLIKTCHKRGVHAMGGMAALIPIKDDPAANAVALEAVGKDKLREVLAGHDGTWIAHPGLLETALNVFETHMPTPNQIFVQKTDVVTEAELVDTNIKGGEITTKGLNANVYIGLNYMESWLRGLGCVPINHLMEDAATAEVARLQLWSWCKHQVTLQDTGKTVTPEYVSQLIDDEVAKCASKPGNKYAIAAKALKDELTGAKPLAEFLTDILYPYIATPGSPIDLATLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR61</accession>
    <name>A0A1E3NR61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14110</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019672.1">
        <property type="nucleotide sequence ID" value="XM_019160229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR61"/>
    <dbReference type="STRING" id="763406.A0A1E3NR61"/>
    <dbReference type="GeneID" id="30176916"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045471">
        <property type="term" value="P:response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16457">
        <property type="entry name" value="RING-H2_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011422">
        <property type="entry name" value="BRAP2/ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047243">
        <property type="entry name" value="RING-H2_BRAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07576">
        <property type="entry name" value="BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="UBP-type" evidence="7">
        <location>
            <begin position="355"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="552"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="72400" checksum="308624389481CDD2" modified="2017-01-18" version="1">MEFSGEGTTRYTLCFDLFSEKDIFESDTRISDIYLETLVQKNVAQPLATFRRSSWIDKVWEKKDVFCPKDNSGEARKIPLSQWSDRLKSTRLKLKRQAPAKADTIDYRFTAVEITPIDPTYIQMSASQSFSQLLPSKASSGSYITDKLCAELLGFGVVRLYKDSEDHVNLFKIDSAGLKHVPGDETTLAILSVPFYFSASDLLLGFFDEEDAKELSHIRLIKSSSPNRFMILLKFRLKDAVKPFMRKYQGRKFNSFEPETCSVVEIKEIVFRPKTKSESEHTKVALPYLLEDPFTNNGDEVPETAVEEARLYTELATCPVCLDRLDSSVTGLFTIPCQHTFHSSCLSKWKDDTCPVCRYSNKIGNVTDNFSSLSLLETSSSPDNNEECMVCGLTEHLWICLICGNVGCGRYDQQHAIEHWKETGHCFSMETNTQRVWDYAEDGYVHRLVQNEADGKIVELPLRDESKKTSDEKVEKIGFEYSKMLIGQLESQREHYENIISNLNTDLAFQKQTSLRFTDKVSWMEKQILDINAKMAAKTKQMQSYEKMGQQFTELKKELADTNALNEALSSKMALIESENVELKNEKSELQEQVTDLMFFLESQEKFKDASDDVKEGQVVMVPKKSKTKSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRT0</accession>
    <name>A0A1E3NRT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_81595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019511.1">
        <property type="nucleotide sequence ID" value="XM_019164989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRT0"/>
    <dbReference type="GeneID" id="30181676"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133493" description="Secreted protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="9899" checksum="F6955FE8E2BE7FAA" modified="2017-01-18" version="1">MSCGLRLSFYVCFFSSVSAASLCSLLLGCGSTIDVSTAFMQAEKTLLPEEGRKEQQQQRQGVWQPSRPSTRTFAATPSAESALLCGPADWAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRU4</accession>
    <name>A0A1E3NRU4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_87237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019925.1">
        <property type="nucleotide sequence ID" value="XM_019165026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRU4"/>
    <dbReference type="GeneID" id="30181713"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="414" mass="44533" checksum="A055259F87C1760C" modified="2017-01-18" version="1">MLSYGRKKLKNTAISYVDSYAPLGLTLGDLSASLRVEDGDGDGDGDAEARRVGAVARDTDTDPDDASELYDRVTARMLNRRRSKLASRASEDVSSNPGSDTDAELSDGGGGASDNDDDTTIEFVIPSQAVSLSDTDDSSIVDLDAFSRGITLLSMALNNELIVKNPKNIRKFTGQAVAHIVNSILDPETLQAIFASLSDETKLQILKYYLSDDLLVDRLKSDQGLHLPGNPTPPSSAASSAASSSASYIFHRRSYSSPRPRNADHTLDQLIISLLQAFYTFSLVLYTRFGIPLLNLLWKFVRTLNENYKLLDIAKALTIRVLALIINLILKLVVHSTNIVQESTLVAMSDSSTPFSAAFAASSPSSASSYTSSSAAGTAGNYARDAFFAVVQQGFVRATQKRHSSTTTPAPEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS29</accession>
    <name>A0A1E3NS29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_24496</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020028.1">
        <property type="nucleotide sequence ID" value="XM_019162355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS29"/>
    <dbReference type="STRING" id="763406.A0A1E3NS29"/>
    <dbReference type="GeneID" id="30179042"/>
    <dbReference type="OrthoDB" id="6224010at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="80" mass="9609" checksum="AEEF17935CAD2BDB" modified="2017-01-18" version="1" fragment="single">LPSWILTPKEEKQVLEEYQKEVWRQCDGYVQEFRKCEAMAGFGVLFKCREQTKAMKACIDNRHQHEFVDEVRDAFIQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS35</accession>
    <name>A0A1E3NS35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Regulatory protein MIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_23743</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in glucose repression of glucose metabolism genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the creA/MIG C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019496.1">
        <property type="nucleotide sequence ID" value="XM_019162320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS35"/>
    <dbReference type="STRING" id="763406.A0A1E3NS35"/>
    <dbReference type="GeneID" id="30179007"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000089">
        <property type="entry name" value="DNA-binding protein creA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ48383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="55" mass="6833" checksum="4E1B43F0C7639AE4" modified="2017-01-18" version="1" fragment="single">RPYKCPLCKKAFHRLEHQTRHIRTHTGEKPHRCTFERCPKRFSRSDELTRHLRIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSA0</accession>
    <name>A0A1E3NSA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum lectin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein OS-9 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_70588</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lectin involved in the quality control of the secretory pathway. As a member of the endoplasmic reticulum-associated degradation lumenal (ERAD-L) surveillance system, targets misfolded endoplasmic reticulum lumenal glycoproteins for degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
            <orientation evidence="1 7">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the OS-9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020118.1">
        <property type="nucleotide sequence ID" value="XM_019163682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSA0"/>
    <dbReference type="GeneID" id="30180369"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045149">
        <property type="entry name" value="OS-9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414">
        <property type="entry name" value="OS-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133483" description="Endoplasmic reticulum lectin" evidence="8">
        <location>
            <begin position="22"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="9">
        <location>
            <begin position="98"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="413" mass="48577" checksum="00BBC42E2463C751" modified="2017-01-18" version="1">MHMPQKVLLALILNYPQLTSCGTNYDKNYLVIKHNEASDFLGYSEDHSNSTLYKVRADNIDYKCWIPFVLDDEETARKHDMDNIQTELKKREAISVIKNFNFQWRDRFVSRNGGYWSYQLRFDYDIRQYHDMGARRENGEPMTVDFKLAAWTDHDQDISLFSKPSYQMTDEKNPYKSELDFEMRTSEDGKKYVTQRIANGEICDLTGLPRSVTISYLCNDKVTVPTIRNVHEWKTCEYSLELESDYFCGHDMWTLPKELISNNVDCYPDMFQGIVSRTFDFEKMSLKPLTGGIFMGWEGENAVKFDLILTKNYKIKDEDSEDDETNNSHYHHLLMDISLGFQTLIRNVQLWRGSERIKWDTIFKLVTELYDIDRSYVGNIQLEQDENGYIVSSFTDEQVPEQSNFVDSEVRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSD7</accession>
    <name>A0A1E3NSD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020180.1">
        <property type="nucleotide sequence ID" value="XM_019160430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSD7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSD7"/>
    <dbReference type="GeneID" id="30177117"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="293"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="842"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1010" mass="111656" checksum="5B8959905712196C" modified="2017-01-18" version="1">MPKAKKTKLPLFDIRVHAVDKDTILLKGAPTEASPTMLSGVIALSATEPLQVKRMKLKLYATLSFRWEEKYHNPKGQVFSHPYKFTKMVYCFDWDPINLERFLHTHDPLHSSFMASRNTSSNSLENLNQNIGFTTGTLTRSNPGSASNLRRIDSNATLNSKESTTSVSGLAAQQKQGQKNSHQPSSHTRTKSASSLSAINLSSLTSLGNSSSSDLVTLEPGNYEFPFQVILDGSIPESIIGHPCCSLIYRLQCSIERGRFSTPIHSRKLLHVVRTLSPDNAELSETIAVDNTWPEKIDYSISVPTKAIAIGSTCQIFLDMAPLCKGLKLGSIKIKLLEYASFSTATGQHTEERTLTTKHIPKIVSNSDGVDIWSDEAPTDEEGVFYRGHNIVLSQDKWNVKTSIQLPPSLEKMTQDLDISSMCKVRHKLKFSVGLINPDGHVSELRATLPVTLFISPFVPIKVKTIEAYDDPFLHSVFEDASNNLQSDNIIFQHEDVNSKALYQRLHQNDTLETNAENLVPTPNMNTQDLMAPPNYDDRIYDRVYDLDSHLDQLDDPITANDDGSTGLPSSQISSTPQTRKSNVINNVNADVNFDVADKGAENEDSRGQGNGGIEKGDQEEEEEEEEEEEEEEEDEDGEVDDTVNSSDFDPFADNTEEKVQKKYLRKYKKPVFSIAGDDDDENPDILDPFDTTPNLSPYHPEIHGRQENVIPGPAFDSGERMPISQFPSSNHLISPGALSPVQHLSRATSFIGDTTGSLPLSSSMSSMSHSSTMDGKVLKEMFNPPSYEIAIHSNASLKDLTPVYDPPPNDLRSNLHIIDSRLRNLRLTGLDQHPHQHRHQQSAPSMSNNSKSTTALNLANKRQSYGLTLLSHGHYQSNTPPTLSRNVSNNSLLSKMMSKTGTSVEDHMIQHTIRTTIPRFELGSPGNRSVVSTPGVMPDGYFSSQDSSSYFSAANSSPNPNSNNVAMGLMISQPKAAHVLGDTTVDMMNENQLSKSSTGLHINLRPHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSN3</accession>
    <name>A0A1E3NSN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14595</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COX17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020222.1">
        <property type="nucleotide sequence ID" value="XM_019160451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSN3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSN3"/>
    <dbReference type="GeneID" id="30177138"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903136">
        <property type="term" value="F:cuprous ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="6314" checksum="859B5C44CC16D10A" modified="2017-01-18" version="1">MSEHKPATESKPKPCCVCKTEKSARDECLLINGIDSPKCETLISSYKTCMKGFGFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSR2</accession>
    <name>A0A1E3NSR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020254.1">
        <property type="nucleotide sequence ID" value="XM_019163717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSR2"/>
    <dbReference type="GeneID" id="30180404"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05137">
        <property type="entry name" value="RasGAP_CLA2_BUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="3">
        <location>
            <begin position="561"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1084"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1094"/>
            <end position="1107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1107" mass="126398" checksum="4162B9DA6C2D3598" modified="2017-01-18" version="1">MSAAPLAKINSSSSEIPTPSPLETEFESNTVTTPTTNAFPNSTSFTSLTQTPPSLLTRNPSTLFDKNLVETLKKLAIKNKGEFRCSNVQWAFGLDDDKLKNNKHVSIKTLLNNSFHWNFVKLLVSAEGKITAGNSLLIVSDIQECGIDIFNFDLIVKLTPKTGNAKSYFFKFTNFNDYLEFLACLLSWHGLKQKGLVSKWNFTNSIIYNDNLKPNEIIVCRFKVFGPLPASSTKVHIDEDGPENPVFPTPTDSNYKEGWFTVIGHLLPTGILNLVSELDGTLLYSINVTLLFNSEIRVLHHSILQNSNVLFLGFIDVLRESHDLHPASGLQFIRDNNFKDVSRILIDFDLRIDLEDWFVALSSFTKLEYVGNQFTGNRLKISEELSLEIMEAQFQLDNHAIANSSNNNFADSLKDSCLYCELILWNTPWFRSAIVKTDQHASTFWKELINLKLPISNSNDFKILIKRAKSTDSYNIDGENSDEIIGTCFVNLELFEDVQFLQKIPIHNLQNKLIGKLMINLSQDETVILPFQKYKSLENILMNSKIESLISYLEPKSSTSTLEAWSIMLLDIYQSLGKEDEYFETLMKYELSPIDSLNNSNISDTLGSHNNSTDNTLNINNSNNSNGGTANNSFRYHTIFRGNSMLSKSLEKYTVRVGHEYLEKLLGKFIEKVRVEDLNCECDPKIEPETYQENYQNLLRYVEYLWNRIYQTTNDIPSSIKVQWKNLRQNVELSVDLNDKETPLNALSSFIFLRFLCPAILSPKLYNLSKTHYSGKISRTLVLIAKVLMTFSNRSKFQKHREPFLIPLNDDFIEKHREEMLIYFDKVTFRKMDFNERVLNMSNIVDRIQLNTSKDILNELPTMPFLIDKYLNIAKLVELLSKFYQAEKEEVGPEKDSIGNYQIEGFDDTGLDDDFLTSLINDENNALNSLLSKHEFKLKDMKKQSAVLLKETESVEKQLIEAEYPHNIDDIWDTYVSCLIRSCRLNIATTSTTPTSMTMVRNSDSDGHVIFDSDLFHNMSLQPNEDELSKLNTFLTSLMVQNEKKKLNKSTLISNRSGSAVSATQLQGNGFGSPEKPKIKLGKKVSESDESGKKKTLFRNIFKRGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSR5</accession>
    <name>A0A1E3NSR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_14536</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020159.1">
        <property type="nucleotide sequence ID" value="XM_019160420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSR5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSR5"/>
    <dbReference type="GeneID" id="30177107"/>
    <dbReference type="OrthoDB" id="10262653at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="119" mass="13545" checksum="0E3F9AF4B2450DED" modified="2017-01-18" version="1">MPKKRASNGRNKSGRGHVKPVRCVNCSRCVPKDKAIKRMTVRNMVEAAAVRDISEASVYDEYPIPKMFNKLHYCVSCAIHSRIVRVRSRTNRKVRAPPVRQRFNQNAKRVSPADAAKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSU5</accession>
    <name>A0A1E3NSU5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial intermembrane space import and assembly protein 40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase TIM40</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_25514</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020189.1">
        <property type="nucleotide sequence ID" value="XM_019162397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSU5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSU5"/>
    <dbReference type="GeneID" id="30179084"/>
    <dbReference type="OrthoDB" id="7481291at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039289">
        <property type="entry name" value="CHCHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21622">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21622:SF0">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CHCH" evidence="12">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="72" mass="8264" checksum="E5FC3866B827E113" modified="2017-01-18" version="1" fragment="single">GEINWECPCLGGMAHGPCGEDFKAAFSCFVYSSQEPKGIECIDKFKNMQDCFRKYPEVYSEELRDEDAVERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSX0</accession>
    <name>A0A1E3NSX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">uS12 prolyl 3,4-dihydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_70803</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3,4-dihydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54160"/>
            <dbReference type="Rhea" id="RHEA-COMP:13817"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
            <dbReference type="ChEBI" id="CHEBI:138052"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[ribosomal protein uS12]-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54156"/>
            <dbReference type="Rhea" id="RHEA-COMP:13816"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020360.1">
        <property type="nucleotide sequence ID" value="XM_019163745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSX0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSX0"/>
    <dbReference type="GeneID" id="30180432"/>
    <dbReference type="OrthoDB" id="430522at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019826">
        <property type="term" value="F:oxygen sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031543">
        <property type="term" value="F:peptidyl-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140311">
        <property type="term" value="F:protein sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000639">
        <property type="term" value="P:negative regulation of SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000014">
        <property type="entry name" value="Prolyl 3,4-dihydroxylase TPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.20">
        <property type="entry name" value="Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019601">
        <property type="entry name" value="Oxoglutarate/Fe-dep_Oase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043044">
        <property type="entry name" value="TPA1/Ofd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039558">
        <property type="entry name" value="TPA1/OFD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE OGFOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13661">
        <property type="entry name" value="2OG-FeII_Oxy_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10637">
        <property type="entry name" value="Ofd1_CTDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="148"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ49247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="639" mass="72689" checksum="6A5DE0F94BDCC612" modified="2017-01-18" version="1">MPEKRDKDAESLDHTGTRKKISIKQTPEEEAKAVEYFQSHVFEPTFKTQLTENIKFSQPFRWGTIKNLISDDLLRNVRKEIMAEIEFTKKETDIYKVFQSGDLANLSAMPADLLARLPSVYKLRSAIYSETFRSFMSDITGCGKLSGIKTDLSIQLYTKGCHLLTHDDVIGSRRVSFILYMPDPDTHWKPHYGGALQLFDSLVPNVPQSDVHSKLVPGFNQIAFFTVQPGLSFHAVEEVKVDRQRLSLQGWFHIPQEGEDGFIPGEQEQTERKSTLQQLESKELREYDFPKLKFAQIPNAISAKNADHLVFSDADREYLSKYINPYLLTVESTTRLAKIFNEESVVDILSFLNEEYESLLKTQMKNLEINEFPPMPQSQSQVQSLFPWKLAIPCHKQRYMYIDGSEYEDIGTAGSIAIANKVKSSSNASNFDLTAQILRLQSQVPSTEYVKSIEDNPNSTTVAEVSAKLCDLASMFKSRAFRKWLSKITELKLVKDNVLVRRFRPGQDFILATKSTGDDNDDDNDDQTMDGLLEATMSLTPSHGWENGEWGAYELCLVDDDAATANAAEEGLNEDEAAIYRTADQDSVVYENQACWNKLSLMYRDPSVMKFVKYVSFDAPGCRWDINCAWKCVDESEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT62</accession>
    <name>A0A1E3NT62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_9728</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020363.1">
        <property type="nucleotide sequence ID" value="XM_019165129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT62"/>
    <dbReference type="STRING" id="763406.A0A1E3NT62"/>
    <dbReference type="GeneID" id="30181816"/>
    <dbReference type="OrthoDB" id="4364at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF2">
        <property type="entry name" value="PLASMA MEMBRANE IRON PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="45518" checksum="E82C98DBCF6A590D" modified="2017-01-18" version="1">MNDIFNVQIFFVVFREALEAVIVVSVLLAFVKQSIGNKNPAVTKKLVRQIWFGTFGGIFICLAIGCAFIGAYYSLKNDIWSKTEDLYEGIMCMIATILITMMGIAMLRVNKMQAKWRVKIARSLVTPPEKKSERMKCGYIVKRYSMFILPFITTLREGLEAVVFVGGVGIGSSAKSFPLPVVCGLFAGALVGGFMYYGGSAISLQIFLCISTAILYLISAGLFSRGVWFFENYVFNLQTGGDAAENGSGPGTYNIHKTVWHVNCCNPLIDEGWDVFNSLLGWQNTATYGSILAYNLYWLALIVTLGLMLFEEKRGHLPFCKSLTLRKLNPMYHVKNKKKNELSDAEAARLFEEARNQLQERVSLEDTDASADSHNVELDVLREGTSANKAESSIEFHTVPLDKKESLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTH1</accession>
    <name>A0A1E3NTH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NDT80 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_14782</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020429.1">
        <property type="nucleotide sequence ID" value="XM_019160528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTH1"/>
    <dbReference type="GeneID" id="30177215"/>
    <dbReference type="OrthoDB" id="4117572at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052605">
        <property type="entry name" value="Fungal_trans_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144:SF1">
        <property type="entry name" value="PROTEIN PACG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDT80" evidence="4">
        <location>
            <begin position="232"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="2">
        <location>
            <begin position="232"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="628" mass="69736" checksum="D4D45B5896E75953" modified="2017-01-18" version="1">MGDNIIGDHSYGGSDNNNSISNNNNGNIDSNNAATSNTIDDEFASLFLQELQNTRAESLKNIPASVTETTAVTGRNHSEVDYMNSMELSASQTNLAMKGAASYAMYLPMETTFNSSFLDLNELYSPGTQQVALESLMKGSGIGGAEDGVQDAGLTGDMMDQLRAGKPSFQNPLYNHGYQQIMEPGKQKEMIDSVLDIATEQGDDVKIKNEQYSPVKEPTVGGKEGGKAVSTESSSENQVLQLISNNVLALMEVNTDAQTTSENFPFNTLCKGNIAGRLYINPESFVKLSKLHNIAALMEEANSTQLFCYRRNWISLDFSMNLSFLETVKNERPFKKLSLHLSSSLRKRDNKDSTKVSVITEKEEFMFHKAEVKNTENIIQASESEVSIEYDDLVKIAQHDGNVHFLWDQIKFKSATANNRHDAANKYYTIVLEVRFHNSQNGGNDTIVLTKRFESHQIIVRGRNPSFYSKKGDILIGKMKKQSSSRACKAEVNHNESTDSAQFTPNLKLATPIENLRTDAVKTVSPADNDYKLPITALGNTVVSAGGGSNKSFSKVKDDNDRHLALKENKGGSSYQYFRVTDNYYLPPVDVGYFPHYVHHSKQVLKSNAVSAGMSHDQEVREQYNYYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTH9</accession>
    <name>A0A1E3NTH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020505.1">
        <property type="nucleotide sequence ID" value="XM_019163775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTH9"/>
    <dbReference type="GeneID" id="30180462"/>
    <dbReference type="OrthoDB" id="3997577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="244" mass="26915" checksum="AFB4C45250DC6573" modified="2017-01-18" version="1">MDFYIVKPNLQIADCEDCPSDSTLLSKKSAYLSLQKQCRKLSQMNGGDAAVPAWCRCKSPGEPRSPSPADNDKGGRDAGGGQGQGQGHGNPVASLPLQSITLAPQDYSKLEPRPDALSLARLDLPNNISSLDGKWLALKYLQLNSDAALVRYKYLKSYAKITRLLKKYFSKPQVSEYEDYSDSEGFRQINDEMAKFKAFENVLTLLVELKKQFHQRFSHFDAACATPADPDEAPACSKTPSSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTP8</accession>
    <name>A0A1E3NTP8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70525</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020046.1">
        <property type="nucleotide sequence ID" value="XM_019163665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTP8"/>
    <dbReference type="GeneID" id="30180352"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="224"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="261" mass="29315" checksum="8DEDF6D14D968127" modified="2017-01-18" version="1">MALENTTSTVLSTIGTVIWCIQLSPQIYFLHKKKDAEGLPPIFMLLWCISGLFMCIYFVVSDSYIPMQVQPHLFTMLCSVAWVQSMYYPPHSYGKKRFLSSAAVFYLCWIGLEVGFCVWLRPVYAHGTHWENLIFGILATVFLCVGLLPPYWELLHRNGRVVGINFLFLALDSSGAVFSMASMCIGTVDAMGMILYAIMIAMELGLFVSQGIWLLRFRLRRTEKDGPGSDVEAVFSDSSSEIRNASDANTEKNPSATVRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU16</accession>
    <name>A0A1E3NU16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NADH-ubiquinone oxidoreductase 24 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14542</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="9">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020166.1">
        <property type="nucleotide sequence ID" value="XM_019160421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU16"/>
    <dbReference type="STRING" id="763406.A0A1E3NU16"/>
    <dbReference type="GeneID" id="30177108"/>
    <dbReference type="OrthoDB" id="10254187at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000022">
        <property type="entry name" value="NADH dehydrogenase flavoprotein 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005725">
        <property type="entry name" value="PRK07539.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01257">
        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26630" checksum="A8E6B1FAC9B2926C" modified="2017-01-18" version="1">MLSALVRNNTRRLVAGPAKTFVRSGHIIAVHRDTKEDNPEIAFEFTAENKKRAEVIIKKYPPQYKKGACMPLLDLAQRQIGFTSISAMNYVAKVLDMPPMRVYEVATFYTMYQRKPVGKYHVQVCTTTPCQLCNSDQVMETVMEKLNLKPGEVSKDGLFSLEEVECLGACSNAPMLQINDDYYEDLQTKEEVAKILDGFAAGKPPAPGTTKRESCEPFSGPKTLTEPPLDVATVTRSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU34</accession>
    <name>A0A1E3NU34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_14662</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020301.1">
        <property type="nucleotide sequence ID" value="XM_019160477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU34"/>
    <dbReference type="STRING" id="763406.A0A1E3NU34"/>
    <dbReference type="GeneID" id="30177164"/>
    <dbReference type="OrthoDB" id="1294322at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002190">
        <property type="entry name" value="Ribosomal_L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="5">
        <location>
            <begin position="5"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="173" mass="20575" checksum="2A3EC01AACDFF62D" modified="2017-01-18" version="1">MANRLREYQVIGRRLPTESVPEPKLYRMRIFAPNDVVAKSRYWYFLQKLHKVKKASGEIVSLNVISENKPTTVKNFAVWIRYESRSGTHNMYKEYRDVTRVGAIETMYADLAARHRARFRSIHILKIVEIEKNADVKRQYIKQFLSKDLKFPLPHRVVKSKKLYAPKRPTTFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU96</accession>
    <name>A0A1E3NU96_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_22185</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020256.1">
        <property type="nucleotide sequence ID" value="XM_019162240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU96"/>
    <dbReference type="STRING" id="763406.A0A1E3NU96"/>
    <dbReference type="GeneID" id="30178927"/>
    <dbReference type="OrthoDB" id="4083952at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029208">
        <property type="entry name" value="COX14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14880">
        <property type="entry name" value="COX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="56" mass="6360" checksum="98597696F9DAED2A" modified="2017-01-18" version="1" fragment="single">MNGYPWYTRATDIAHRLTVLGLVGFTVYMSAGVGYTLYQNGKMAEERLAKQKEVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUQ7</accession>
    <name>A0A1E3NUQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mob1/phocein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020491.1">
        <property type="nucleotide sequence ID" value="XM_019163771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NUQ7"/>
    <dbReference type="GeneID" id="30180458"/>
    <dbReference type="OrthoDB" id="10261121at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062200">
        <property type="term" value="P:RAM/MOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000100">
        <property type="term" value="P:regulation of establishment or maintenance of bipolar cell polarity regulating cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="15"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="38296" checksum="D20BBB644A71D71D" modified="2017-01-18" version="1">MGEFMLNINTRKLKFNEPNFGNDSSPSLGNSNSLYSNSNASGASLRKIQSPSKQSVRSYKYSQMQQQQQQQQQQQQQQQQQQQSQHQQNLQFNDQPLFHVQQHNVPENANQAPPLFMCEPFIKTALVKGSYKTIVQLPMYVDYHEWLALNAFEFYNHLNKFYGVIAEYVTPDKYPTMNADPRTEYVWLMPNNRSASLPANQYIEYALTWISNKINDQTIFPTKSGMAFPPSFLRDIKGICRQMFRIIAHIYWNHFEKIVHLSLEAHWISYFSHFISFIREFDLMEAKELEPLSVLIDNLEAQGRIIKLENTQSYQKLQHSTNSAGTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV08</accession>
    <name>A0A1E3NV08_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_100754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020496.1">
        <property type="nucleotide sequence ID" value="XM_019159624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV08"/>
    <dbReference type="GeneID" id="30176311"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="102" mass="12120" checksum="ADADD7614AF88903" modified="2017-01-18" version="1">MRDRERLIHVNGTRCTDEKRKHPLQRRVAAYLRCEPQPRPKRSSDMRGRESLRMRMRKRQRKAKSQRVVDWFRARYLFPGILCVPASKIIQSVLTSPSATLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NCZ3</accession>
    <name>A0A1E3NCZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">nitric oxide dioxygenase</fullName>
            <ecNumber evidence="4">1.14.12.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_18528</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 nitric oxide + NADH + 2 O2 = 2 nitrate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19469"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 nitric oxide + NADPH + 2 O2 = 2 nitrate + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.17" evidence="4"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015167.1">
        <property type="nucleotide sequence ID" value="XM_019162044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NCZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NCZ3"/>
    <dbReference type="GeneID" id="30178731"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070458">
        <property type="term" value="P:cellular detoxification of nitrogen compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06184">
        <property type="entry name" value="flavohem_like_fad_nad_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF3">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="15">
        <location>
            <begin position="11"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="158"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ44054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="389" mass="43535" checksum="B2D4EEF5D71F4CB5" modified="2017-01-18" version="1">MASKQIFKIVPLTPSEISFLQSLAPAVKEVGVKVTSTMYQYMFQTHPEVKSYFNMTNQRTGRQPKVLAFSLYQYILHLNDLTPINGFVKQIVIKHCGLGIAPEQYPVVGSCLVHAFKEVLGPEAADEHFVNVLTKAYGNLAQTLIDAEESVYQGLQWRGCKDFKVVKLEKEAADVTSVYLTPVDGTKLLPIIPGQYISLNWDVQDETVQPREYSISHNLINNEYRISVRKLVNGKVSTFVNTKLQVGDVVPVHAPIGNMTYDATSGKPKIAVLAGGIGITPMITIMQAALKNGQSVELFYSNRTVDSEPFKKTFENWRSEYPDTFKLKNYISGNGKKLTISELEHIVPSEYDVYLLGPVGYMHEFKTYLTNKGVSDVKMEFFGPTDPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND92</accession>
    <name>A0A1E3ND92_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_37702</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat AIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015204.1">
        <property type="nucleotide sequence ID" value="XM_019163100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND92"/>
    <dbReference type="STRING" id="763406.A0A1E3ND92"/>
    <dbReference type="GeneID" id="30179787"/>
    <dbReference type="OrthoDB" id="2306at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003786">
        <property type="term" value="F:actin lateral binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032466">
        <property type="term" value="P:negative regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000102">
        <property type="entry name" value="Actin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000167">
        <property type="entry name" value="Actin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856">
        <property type="entry name" value="WD-REPEATCONTAINING PROTEIN WDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="517"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="560"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="594" mass="66092" checksum="A6B300961E71A472" modified="2017-01-18" version="1">MSITPKKTIVGNPSTKRAFTTQLSYDKTRNRLIYPSGKCVVLRSLDQGGESWVFNGHKYPVSVAKVSPSGFYVASGDESGNVLVWDCSQPEMIVKSEFKILSNRINDLAWDADSKRIIAVGNGSDTFGHCFTFDSGNSIGEISGHSSQINSVAIKTTRPYSAFTVGDDSNVVFLKGPPFKFNQSNKTIHTNFVKMVKFSPNGKLAASVGVDRIIALFDGNTGEVVKSFKDLSKGGIYAVDWIDDESFLIASADAYLRIVNIEGKVVKEWNLKYQVESQFLGCAVVGEDKFIGLTLGGTLYVFSKESSDPVEIINAHQVSVTSMAKLKESDEIFTGSYDGKILKHLVENNEDTLITGDEHKGLVVSIKEIPDVSKSLFSISWDDKLKLISERLEISTVDDLKKQPVDLKLNTKFAAVLFEDGIRFYDFNGKPISEKRLDYEASSIDVSEKFIIVTDAKNFQVHIYDTDLKVLNEVNYLRSKPTVLSISSNEEYLACGDIQGKILLYSLTDYTLITSRWAFHTSKINSIKWSPGDRYCLSGSLDTNIFIYSVEKPSRNIKFLNAHKEGVNCVEWINEDVIVSAGSDSCIKYWDIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDC0</accession>
    <name>A0A1E3NDC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Clp R domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18572</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015230.1">
        <property type="nucleotide sequence ID" value="XM_019162063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDC0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDC0"/>
    <dbReference type="GeneID" id="30178750"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000017">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF176">
        <property type="entry name" value="HEAT SHOCK PROTEIN 78, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="132"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="538"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="8">
        <location>
            <begin position="701"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="343"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="815" mass="91853" checksum="547D49D9CE4F9B3D" modified="2017-01-18" version="1">MLQRSTCRLLVANNSKKLLGFTKANIQPLSTFTNRSPLLHGGASAKHAHSSVPGQGRNLFTSPGTGFSVCRSFHTSTPKFVELRMNKDQQEEPALKKYATDLTEMAKDGKLDPVIGRDEEIKRTIQILSRRTKNNPVLIGNAGTGKTAILEGLAQRIIKGEVPESMKDKKVIALDLGSLISGAKYRGEFEERLKAVLSELNETTILFIDELHILLGLGKSEGSIDASNLLKPALARGQLQCCGATTIDEYKKYIEKDVALARRFQPILVNEPTVEDAISILRGLKEKYEVHHGVRITDSALVTAAVYSNRYITDRFLPDKAIDLVDEACSALRLQHESKPDAIQELDRSIMTIQIELESLRKETDEISGQRRKKLEETLKIKQDELERLTKIWEDQKKELDRIKQLKLEIEQSKNNLEVMRRENNFGEASKLQYDTIPSLENKLNDLIKNQEENAQNDDDPVANKSMLHESVSSDDIARVISKATGIPVQNMMRGEKDKLLYMEQALKGRVVGQDDAIESISNAIRLQRAGLTDEKRPIASFMFLGPTGTGKTELTKAVADFLFDNENSIIRFDMSEFQEKHSIMRLIGAPPSYVGYEEGGELTEQVRRKPYSVVLFDEFEKAHKDVSKLLLQVLDEGKLTDSQGNKIDFRNTLIIMTSNIGQDLMLQDKPNKTEVLDVMKSYYSPEFINRIDEVVVFNKLSKEALRNIVDLRIDELQDRLVDRRIELSLTDEAKDWLTKNGYEPRYGARPLNRLIKRKILNPMSRMMLKGDVKMGDKVDITVDQSNDDLLINAIHVDSNAAPAPAPPVSPTDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDI5</accession>
    <name>A0A1E3NDI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dihydropyrimidinase</fullName>
            <ecNumber evidence="7">3.5.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18444</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouracil + H2O = 3-(carbamoylamino)propanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16121"/>
            <dbReference type="ChEBI" id="CHEBI:11892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15901"/>
            <dbReference type="EC" id="3.5.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="8">Carbamylation allows a single lysine to coordinate two divalent metal cations.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.2" evidence="7"/>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015295.1">
        <property type="nucleotide sequence ID" value="XM_019162004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDI5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDI5"/>
    <dbReference type="GeneID" id="30178691"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004157">
        <property type="term" value="F:dihydropyrimidinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000217">
        <property type="entry name" value="Dihydropyrimidinase-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF1">
        <property type="entry name" value="COLLAPSIN RESPONSE MEDIATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="9">
        <location>
            <begin position="52"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="8">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR611778-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="496" mass="55251" checksum="BB5C30A8742EB51D" modified="2017-01-18" version="1">MSYDLVIYNGRIVTASDILPSSIYIGINNGIIETLTTSKLSGKREIDAEGGYIMPGGVDSHVHIDQDNAPAGDNFESGSRSAVAGGTTTIIPFAIQPKKEYDLLKVVEEYHQRANKHGGSYCDYGFHLIISEPTPEMLEKTFPAVCNKEAITSVKVYTTYPRYKLSDEQLLQIFLTNRKNNVTTMVHAENSDIIDFINNKLMSKKMTDPFFHTVSRPQLAEDEASYRAISLAKVIDVPMLIVHMSCESALDHVKKAQTDLLPIFAETCPQYLFLTGDYLKHDCCHHSSQGDEFQGAKYICSPPLRATQQDLEAVWKNLINGTVTVFSSDHAPSNYDHPLGKKKGLVNGIPHFKDVPNGLPGIETRLPLLFCYGVEKGRITPQKFVELTATNPAKLYGLSETKGTIGVGLDADFTIWYPEGKMERFSLTNDMLHHSIDYTPYEGMEFTNWPRYTILRGNVCFDRDNGGVVGDKVGNYLRRKGSVLAGSRAKLNPIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDT1</accession>
    <name>A0A1E3NDT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015408.1">
        <property type="nucleotide sequence ID" value="XM_019160070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDT1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDT1"/>
    <dbReference type="GeneID" id="30176757"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="2">
        <location>
            <begin position="10"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="691" mass="74691" checksum="0D2DE1B2F8466EE1" modified="2017-01-18" version="1">MKLIPGNGYYDYLLQVGEHKQDMGLRIDILQGDVWLPASSAFIPCSATASAGSNYTYTVTSISYTSTLFTGPTTIDSATVTMMPWETQASSQSGTSTSSTIELTVSMIEPVFGRSCASIGVYDLLESAYAAFIDIYTSLEVSVDDATSYLKVYLSDILVSGVWAVDNFIMSFKSPKLETLEFSNVPFVYANFSNVGVGSLAVGISQSENGFKNNFISNFVINGLIGSNSYSLALGANNSTYPQLILGGVDKSLIFNDPDSQDNSSSKMALFDFIPVQDESGMYISENGGLSTTVPAFPIYSWGVTSSSSGQSITFSPSYNDRTRIANYPKPALLDSRHNYNFIPYSTLVELAIELNAYYSSDLGIWLADCSVGDTGTIDMYLGNYTVHMAISNFLNPALVNNTNLVFSSGDPACYLTFLPDYYLGYSLMGTPLLKNIYLAIDNENRQLGISQLQDQLKDTENSSSQGTHVIGNGILHMDVETVRAPKTSFEEDQKAAEVPTAESHFSHGVTPLSQQQLSRTMTITTSTTVDESRVSAYKTVVTVSNVFSSATATSEPEFYAIESGTIPFAQKFVTVTSLTLTIPTNVVFTDTSVQSTEVYISGGEVFLQTNESNGADTQTKKTQSTETDSTFYAFSSLVVSKISSNRVSGAGSHVQPPSLLYFEASSGEMHYGLKVAGYFSALVLCVAVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE83</accession>
    <name>A0A1E3NE83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_26384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015519.1">
        <property type="nucleotide sequence ID" value="XM_019162446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE83"/>
    <dbReference type="STRING" id="763406.A0A1E3NE83"/>
    <dbReference type="GeneID" id="30179133"/>
    <dbReference type="OrthoDB" id="653904at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017055">
        <property type="term" value="P:negative regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22906">
        <property type="entry name" value="HFD_DRAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF5">
        <property type="entry name" value="DR1-ASSOCIATED COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="3">
        <location>
            <begin position="10"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="89" mass="10016" checksum="1E1154F27860C151" modified="2017-01-18" version="1" fragment="single">LKGYRQMKTHFPSARVKKLMQSDEDIGKVAQATPLVVGRAVELFMCTLVDKALQESRKGHSKKISVLALKQAIDSNEEFDFVAEVCDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEJ5</accession>
    <name>A0A1E3NEJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_74274</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015655.1">
        <property type="nucleotide sequence ID" value="XM_019164726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEJ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEJ5"/>
    <dbReference type="GeneID" id="30181413"/>
    <dbReference type="OrthoDB" id="1928087at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051294">
        <property type="entry name" value="HORMA_MeioticProgression"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225">
        <property type="entry name" value="HORMA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225:SF7">
        <property type="entry name" value="MEIOSIS-SPECIFIC PROTEIN HOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HORMA" evidence="9">
        <location>
            <begin position="17"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="515" mass="58917" checksum="E07DBCDE8508F4AB" modified="2017-01-18" version="1">MKNFIYRKRASERVTPSRSEQTIIDLLLMSVSCIAFLRGFFTDKFFIDDVFEIPKDPASPNLSKRDSIKIKRIKAGVSDEADMILRWIDTSVFDALHKRYLRSINLSIVLDENHPSDVYESYNFDVDYNDSSNESITFNEEVLLTPQEITKLQIFKLLKRFILLTQSLPALPPKRFLFMRLLFSDRCPTDYHVDHFTDCSDESSASIKIPLPVYHDIIANCGEVNSVHHQISTSLVSLSNLATTDFENAKIMKLDPFSISSSSYYNVSNKVPEVIDSQVSQITKDLYTVVKNYDAKIHDGFTQIPSVQESEPSIECSCKSRMYLQYSSIVQCDTCYRIMHKVCYSVTSDNSRSFTCIDCLPDKRTINFVDMLIIFNIRKLITLLQHNKKTSLKSITEAAVLLGYDLNDFSSDESIQMSIVNSFSILIYQGIFSLKTHKSFSHNVFKVDVEGLICNNSKIKKGKYFLSYVTKNNHQKINKFIDPNFGKENEINNMNESVASQIGDETIVEDTATKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEN6</accession>
    <name>A0A1E3NEN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_22224</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015669.1">
        <property type="nucleotide sequence ID" value="XM_019162243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEN6"/>
    <dbReference type="STRING" id="763406.A0A1E3NEN6"/>
    <dbReference type="GeneID" id="30178930"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="241" mass="27640" checksum="A2EAB9D50E245DE0" modified="2017-01-18" version="1" fragment="single">NYGFDDKRGNYIISIGDHIRYKYEINSTLGNGAFGSVVSVSDHSSKSKIVLACKIIKNDPRWSLQAVEEIKILKKLNHPNIVKYVEHFTFRTHMCIVTEILAITLYESIQLTNYKGFSLNLVKKFTREILQGVAYLHSNNIIHCDLKPENIMISGDGTIKIIDFGSSCHTDSLKYSYLQSRFYRAPEVLLGGRYDTKMDIWSIALISLEIFAGVPLFQPQNEWELFSLCVKFLNIPSRKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEU2</accession>
    <name>A0A1E3NEU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74355</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015755.1">
        <property type="nucleotide sequence ID" value="XM_019164746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEU2"/>
    <dbReference type="GeneID" id="30181433"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="186"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="374" mass="40685" checksum="3FE1F6AEC1FD2914" modified="2017-01-18" version="1">MSSKSKYQKVDTTDNFHVGPDLMDDDDDDIFITPDIEAPKSHLPPKAQKTEAPKPQPAVNTARPAVGTSANANASQGGSFFNLSSFITPQLPSFVQIETRERPFSGGNTLDESVLTTLHRDLMTIGDKLLSILWPLRLRQSLIAVKKISNRFSPGSGDVEDSIGGSGGVGDSSTSLGSGGEEYTSETMGKIRDWDLWGPLVINLGFSLIITYLQTRSLNTGGENSRGKDASSQTFSAAFTLIWASLAVLSINIQLLSPVQQKLENGGMTNGVVGLSFFQCISLLSYALFPIALGGMSSIFIPFKFVRLIINVVMLFWALLCTWLIMAIASNCKRKGSLTLVANDDDNQGDQRIFLMVYPVFLVFGLFSWFCVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEV9</accession>
    <name>A0A1E3NEV9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_174287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015739.1">
        <property type="nucleotide sequence ID" value="XM_019161599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEV9"/>
    <dbReference type="GeneID" id="30178286"/>
    <dbReference type="OrthoDB" id="3995942at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="201" mass="22502" checksum="CE4F7AFBE8AFA316" modified="2017-01-18" version="1">MDLGYSSSDSDTDDENAKKRRHDEDAIDGGNKKEEEVTSIDIDIRKVFHLHSTEQSKRRKLDDRSGKISRAISKVLNDKVEIDSTTGDDGELDGGESESAGKKGVKDKLGIPKDAVVAELDMSDFYKHNQEELESGRLNAERQAKIGDIVYHREAGHSNAGRLDRVIQFNLNNEEKIMYQNKERIAKEMAIAREKANAGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEX4</accession>
    <name>A0A1E3NEX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_19890</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015810.1">
        <property type="nucleotide sequence ID" value="XM_019162145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEX4"/>
    <dbReference type="STRING" id="763406.A0A1E3NEX4"/>
    <dbReference type="GeneID" id="30178832"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048680">
        <property type="entry name" value="COG4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20663">
        <property type="entry name" value="COG4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="9">
        <location>
            <begin position="164"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="871" mass="98836" checksum="7BC307C56E521AA5" modified="2017-01-18" version="1" fragment="single">LDSTLTTTVNIKQLSKLLNDIKVTQSNIDSTLQSYTTNNTFQHQSTIRSLEISRVELSTTLNHSRNLKGIMDGANSLSYKITERVRLLDSEKSQLSQIKCFVDNVKTLKLELLRAHDAIERQDWLIASKSIAIIRQLPEGLLTDPYVEFKVPTTSLEELPSMLLEKWIKQLEDTFVQEFNDAAAKRDAQKLTYFFQLFPLIGKDKVGLRCYSKFICGIISDQSRSTIRNAQNKESKPEFYAQLLFRLYQTISGIVNQHSKVISSYYGKAVVNDILKDIQMECDLQSNLIYETYADSKHLDRYASEIQQYGYPILIKEIYKNVAESEDEEEENDDESEAVELTDVSQVTDELSAMMNHWAMYSKFFVVVWNGFLDGKSNNSSSIDNNKNIFPSPLILSSFGIKIHDRIIRQFDLFCTYSVRRTLEKACSIESLGSLLPQFSVCLKFLTLVFKKIDNSTTHSLYSLIPEDPAISSLADDIIIVLNTILLEVLATGELTTIKNMVSNIKRILLNDFLNIIQQRLKGLSLKSSSNLLTKNTIQKIHQQQNPLFESAFNDSSISTPSRSSTPVGINVSASDIANTGSLFMRSLNAAISYTMAGEGEEDLTYLTGDDTDIKQYVIYLNTLAVMNTYLDRLMDSCMKSVENNSLLIVDDQELTNVRRSQEETLTTDFDMRVPSDESIHSRIGGLLRSIPAGFSERANDLIDSNVRLIFERVLKTRIIKLVNESISENYLMAIEEGQYYIEDADRITRFIKNWNSLIIPYATTLSNSVFNRLIAKIVDLSAIILENKVWQLEKKVSAVGAIKLEQDISTVIGELTKFNYGLRSAFIKVTQIIMVVGLDEEEDVTALEDIGDEIEWALTPSERTRARRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF43</accession>
    <name>A0A1E3NF43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N6-L-threonylcarbamoyladenine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_18163</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015888.1">
        <property type="nucleotide sequence ID" value="XM_019161899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF43"/>
    <dbReference type="STRING" id="763406.A0A1E3NF43"/>
    <dbReference type="GeneID" id="30178586"/>
    <dbReference type="OrthoDB" id="10254073at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24132">
        <property type="entry name" value="ASKHA_NBD_OSGEP_like_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000038">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000295">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="11">
        <location>
            <begin position="53"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="155"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="356" mass="39443" checksum="4385AA8497985D06" modified="2017-01-18" version="1">MKLDFDTRAKKGQKWYIGLGMEGSANKLGIGVVKQPVGNPAIKENRAVIMSNVRDTYNAPPGQGFMPRDTARHHRNWVVRLVKQAIEEAGITSSDIDCICFTQGPGMGAPLQSVVVAARALSLMWKVPLVGVNHCIGHIEMGREVTGADNPVVLYVSGGNTQVIAYSRQRYRIFGETLDIAVGNCLDRFARVLKIPNDPSPGYNIEQLAKGGSKFIELPYTVKGMDLSMSGILEYIEEIVRRGTEKVEDLCYSLQECLFAMLVEITERAMAHVNSRQVLIVGGVGCNERLQEMMGIMARDRGGHVYATDERFCIDNGVMIAQAGILEYRMGAECAVEDSICSQKFRTDEVFVRWRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF48</accession>
    <name>A0A1E3NF48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74455</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015865.1">
        <property type="nucleotide sequence ID" value="XM_019164775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF48"/>
    <dbReference type="STRING" id="763406.A0A1E3NF48"/>
    <dbReference type="GeneID" id="30181462"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000907">
        <property type="term" value="F:sulfonate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044273">
        <property type="term" value="P:sulfur compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="85"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="400" mass="46326" checksum="9DD68D21F2730F43" modified="2017-01-18" version="1">MTLEPGRTYGASISYTKDDDSKIGDVFTVSKSNREALKFPRWAPSWDPKEDHKFEHLKPFRHIDRGLFGDPTFKSLREDNKDVTFRKVSPKFGLEVDGIQLSTLSNKQKDDLALLVETAGVVAFRNQDFKLQEFDKIKDWAKYYGPLDVHATSGAPINHPEFHIVFKRSDPDHHLKVFEQTLNNIYWHSDVSYEPQPPGVTLFAMLETGLGGDTQFLDSFEIYDRLSPLMKKKLEGLKVLHSSKEQAAQNTFEGGIERKKAVESIHPLVRYHPVLKRKFLYINSVFSKRVLGLKAEESENLMSFLNKHSASCLDAHVRLQWDENTVVLWDNRRVLHTGTSDFNTTDIRHAFRVTTMAERPFENEQEYENWTPEQEEENIKLKDHYLGLSPAQYYEETSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFD8</accession>
    <name>A0A1E3NFD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">T6SS Phospholipase effector Tle1-like catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015978.1">
        <property type="nucleotide sequence ID" value="XM_019161585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFD8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFD8"/>
    <dbReference type="GeneID" id="30178272"/>
    <dbReference type="OrthoDB" id="3162439at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF2">
        <property type="entry name" value="TLE1 PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="T6SS_Tle1-like_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="1">
        <location>
            <begin position="4"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="588" mass="67429" checksum="0F3CA1A93379D7A2" modified="2017-01-18" version="1">MTNNIIMCFDGTDNNFGTKSPSNILELYKLLEKNSPNQHCFYQPGVGIDIDLSQTSFQHWASFPFHNPIKNTVDSAIAFTIDYHVIQAYRYLIEKYQPNDKIYLFGFSRGSFVARILAGMIEKIGLLDSGLESMVKTAWEIYKTWEQIGQPTDLSNPAKCSYSLRLFKQTFCRYDVSIEFMGLFDSINSCGIIFDRLFPFTSNTSNVKHIRHTVSIHERRSKFKQNLFVPHTYLPSFLNSKHSCDSLSSLLSQQSNNDSVPSILSVPNEDSVTTSHQVSARFSKKCSSDLLEVWFPGDHSDVGGNWPYDNNGNRISNLPFRWVLSYAVEFGVLFKTNSLEEFNSKFSPLKSSLSFSHDILSFKKYKYPEYVSPYCLPTDDEEAQLATRDESHVISTHDTNGSHVFSIRSPLFPTFPRYSGIDTGTEGGKLDTFKGRGDESIISTLFWWMCEIIPIGYLVENQQGKWRPLYWPNLGENRNIPFNAKVHWSVLWRMKFVKDRHYANLPAIFKVIEKIIQQEDEAFNTEDNEIITLTLTDFVDLKTGTIRADIEHVIGSALKRNKQISLNIDWKNPPNELSYKTKTRNNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFG8</accession>
    <name>A0A1E3NFG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18949</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015441.1">
        <property type="nucleotide sequence ID" value="XM_019162095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFG8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFG8"/>
    <dbReference type="GeneID" id="30178782"/>
    <dbReference type="OrthoDB" id="3997666at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF95">
        <property type="entry name" value="CYTOCHROME B5 TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="69" mass="7684" checksum="142100BD17F25B92" modified="2017-01-18" version="1" fragment="single">INKVRLNEKPDGIWMMIHGYVFDIVNLLEHHPGGAECLLDCAGVDATRVFDDVGHSDIAWEMLENCCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFH8</accession>
    <name>A0A1E3NFH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysophospholipid acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015196.1">
        <property type="nucleotide sequence ID" value="XM_019164913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFH8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFH8"/>
    <dbReference type="GeneID" id="30181600"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071618">
        <property type="term" value="F:lysophosphatidylethanolamine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036151">
        <property type="term" value="P:phosphatidylcholine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090640">
        <property type="term" value="P:phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="72679" checksum="09186482521DE051" modified="2017-01-18" version="1">MVDTLNPFRPLVGYLCEASGLDAASVKTLVCLFLSFPVSAVFKRLPDTSYRLKNWYIIVASAVYIFCILEIYKGFFVILLNALFTYFLTKYYRSRFMPWVNLVVLMGVLCVNHVQAQFDIRESNPGEIDFTGAQMVMVMKLSAFGWSYWDGLMYHQDKDKFESNLNVYQKSRAILHHPGLSSYLGYVFFYASLITGPSFDYADYEKFILTELFDDVPESKQPGRRRKRKIPKSGRVALRKVLQGFVWIGLFVYLTPLITVDYSLSTEFRTGKSFLYKVAFIWVLGIVERLKYYAVWLISEGACIVVGLGYNGYNPEKDVMYWNRVQNIDPLRFELGQNVHDCLEAWNMNTNKWLKNFIYLRTCTRDKKTGKLKAGMIPTIITFATSAFWHGTLPGYYLTFILGAFLQSVGKVFRRNFRPIFATKDNSNVSPYKVLYDIVSWTITQLAFGYAVQPFVILEFGKSIALWRSCYFWVHIGCAVVLAVFSGPFGKQASLFMKKYHLHSKEVKKPEESPVPIAIQLATLKDQFDSTTDLLEVLKSSNNSKTAAAELPDFDNQEELQDSTVLEFDNLENDFNKLSKEYAEWRDQSLQGKAPGQITEEEVSRMKLALTNIERDISAFVAAMKPASSTEPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFW8</accession>
    <name>A0A1E3NFW8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015571.1">
        <property type="nucleotide sequence ID" value="XM_019161966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFW8"/>
    <dbReference type="GeneID" id="30178653"/>
    <dbReference type="OrthoDB" id="4093447at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="541" mass="57741" checksum="1EE1FDB4748405AE" modified="2017-01-18" version="1" fragment="single">MDMSITPTTSYPDMDVGEDVYSTASAAGKAIQPPPHLHARNRNDSLISIDTTYTVRTNVEQTHFPPTAGQPHLGFGRNRQGSTTSLSSSVFSEMPPPQMDTYSFTPVFDDELYSYYLSYAQQPNITPFDTRFPPSGILSLISKLFLEHCILANKVQIDSRPDVNAELVADSNRHKCLAIIRLRLIHICNANLNAAEDQYMYTDQLPISRTNSIASAISLGDRVIPGISAAPGGGPATGEVFPQQIPLPMAHRPSWLHMPNLYSTSSRGDSNTANHMLSSTDSLVDRVPASLSFTPGTNTNTIPTANPLNENADYTTPTGSTLLQGFHNDSNGSGNADFAQTTPPNTGVSSKRGKPTLNLHMPSFLKYRSSQYHNMNAMPPRPLFSTGPATPGTPGTPNTPIGSTSSTQRNPRGVRSGSLSNQGANYFNLTPTNGSNSAKNFLNPALHHLQGPNGGNFGSSSDLHSPFEQACPSPLAFSAPPGGVLSGSNEVSTPTMTTTPLVSSVSTPTTAAASVPNPSTLDLYNATVARKRDSLNLKRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG26</accession>
    <name>A0A1E3NG26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SBDS family rRNA metabolism protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_59865</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016149.1">
        <property type="nucleotide sequence ID" value="XM_019163396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG26"/>
    <dbReference type="STRING" id="763406.A0A1E3NG26"/>
    <dbReference type="GeneID" id="30180083"/>
    <dbReference type="OrthoDB" id="10253092at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990932">
        <property type="term" value="F:5.8S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018023">
        <property type="entry name" value="Ribosome_mat_SBDS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF1">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01267">
        <property type="entry name" value="UPF0023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="8">
        <location>
            <begin position="15"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="9">
        <location>
            <begin position="110"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="28625" checksum="6987D84E8B1DA6CE" modified="2017-01-18" version="1">MGKINQPNTQIKLTNVSVVRMKKGKKRFELACYQNKVHDFKSGVEKNIDDVLQINQVFMNVSKGQVASSEDLKAAFNTTDIDSIVQEILKKGEIQLSEKERAQQNEKLHNEILSIISAKCINPKSKKRYPPTMIDKALKELKFNFVNARSGKSKALEAIKLLVEKQVIPIARAKMRVQLRMSDKEYKKISTEFQAEFVSVLESEDLKDDVIDLVFLVEPENYKQLVEKSKQINGKLELLDTAVIQEGETKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG39</accession>
    <name>A0A1E3NG39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-dependent RNA helicase SUB2</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-dependent RNA helicase sub2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PICMEDRAFT_17611</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ATP-binding RNA helicase involved in transcription elongation and required for the export of mRNA out of the nucleus. SUB2 also plays a role in pre-mRNA splicing and spliceosome assembly. May be involved in rDNA and telomeric silencing, and maintenance of genome integrity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016228.1">
        <property type="nucleotide sequence ID" value="XM_019161667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG39"/>
    <dbReference type="STRING" id="763406.A0A1E3NG39"/>
    <dbReference type="GeneID" id="30178354"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17950">
        <property type="entry name" value="DEADc_DDX39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000809">
        <property type="entry name" value="ATP-dependent RNA helicase SUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="23">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="79"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="22">
        <location>
            <begin position="266"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="19">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ODQ45115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="432" mass="49032" checksum="32EFC97F376FEEE7" modified="2017-01-18" version="1">MSAEGEEELLDYSDSEEVNNQQQAEGGDAAVKSEETDKKGSYVGIHTTGFRDFLLKPELLRAIADCGFEHPSEVQQSCIPQSILGNDVLCQAKSGLGKTAVFVLSTLQQLDPTPGEISTVVICHTRELAYQIKNEYTRFSKYMPEVKTEVFYGGVPIQKDIEILKNKETCPHIVVGTPGRLNALVRDNLINLKHIKNFVVDECDNVLENINMRRDVQDIFRATPFQKQVMMFSATLSNEMRPICKKFMKNPLEIYVDDEKKLTLHGLQQYYIKLAEEKKNVKLAELLDSLEFNQVIIFVKSTKRATALNQLLVESNFPSIVVHSGIPQEERIQRYKLFKEYNKRICVSTDVFGRGIDIERINLAINYDMPSEADQYLHRVGRAGRFGTKGLAVSFIAAKKDQEILDNIQDRFDVKIQEFPAEGVDPSTYMNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG90</accession>
    <name>A0A1E3NG90_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016273.1">
        <property type="nucleotide sequence ID" value="XM_019161679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG90"/>
    <dbReference type="STRING" id="763406.A0A1E3NG90"/>
    <dbReference type="GeneID" id="30178366"/>
    <dbReference type="OrthoDB" id="411211at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="403" mass="46496" checksum="74220AB97EB8F767" modified="2017-01-18" version="1">MGPTCEGFYDIQKSSYFLSYCGITFKFDNVKSSHNTNEVLNTMSKELNCSAIFIYQNTEKDTNTFLWNNYTELLLNTLRHPPSINYVKDLDQFTPSICDDDKGKIKIRYSIYDCSHAGGLEIKFNKHSLGLSSFKIELGVTTLQEMTRVFGLPSDSMLKRKSRSNCIQMKKFRSKDNNDHVFEFTSSRAYLPTSMIEPRYDYEDIIESITSTSNALENFNQETVKIHNYFNFGFDVIYDLNATKNGTNVVSRIILHQNRVRSVDFLKYEKLTVFLRNQNKELVTNLKLNEISNEVNLSGLPVFLDRKEYNIQENFESELKETDRIFEFVNIDEEEIEKENGETQVSGGRIDEDKDADSEENSKDKENENADLKYWGLTNYDGGNGAIFEALSNTGDLCTITLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG96</accession>
    <name>A0A1E3NG96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_12867</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016245.1">
        <property type="nucleotide sequence ID" value="XM_019159978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG96"/>
    <dbReference type="STRING" id="763406.A0A1E3NG96"/>
    <dbReference type="GeneID" id="30176665"/>
    <dbReference type="OrthoDB" id="10252740at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="364"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="634"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="987" mass="109009" checksum="9D8061174F865DC8" modified="2017-01-18" version="1">MSGGNYRGRGGARGGARGGRGGARGGRGDSRGGRGGGRGGRGGRGGRGGFGGGFDASDAQVIKPEYNFKPEYESEVTQPDPLPKIQDLIVAEEKERKPEDPYQLVRRPGFGSVGIKTKVGTNYLKYQITGMSFWYYNVTFQSEINTKKKMKQDLLEILLNKSPFQGLKSKISHNGSDALYSSAPLPISKEDEKVRFDFEPEEYRGVISSTVLTTLKSSGKKKGNSADEDPQVYCTVEFIHKLNMDDLNNWVQINDKKNIEPNAYISALNVVLGYQISNCSNVFMAGKSRFFFVDHPEKCQSFQKGLYIASGYYASVLPSFDNVMINIRPVAGAFIKSHNKDGSPMSVADLIADYFGETDLRKVSSNEIMSQKWFFKGIKIQRTYLGHKSKPKGIFDISKSETANNYKFDCDGKQTSVAQYFESKYNFKLKYGDAPLVHLGGSNYVPMEACMILPGQEFKGEITDVKGVLSFSTHRPHVIAGLVQQDGVKKLTSSINPEESKRIGTKLVVVPSRVLSAPVLEYKNAKIIFTEKQADGKSEKAKGSWDLINKQFYEPVKGVKKLTVLVLENTRRPLRGEEKSDIEAACNEFVAEVGKHGVMFNKKFAIKAVSYDRVENLTREIVSIMKPLQASTDYVLTILNQKEKAIYAAVKTALDRDLGILNQCTIGNKFARRKFGKFDIQMYALMSMKTCIKLGGANHVLSSADVGKLVVNGLPALLLGADVTHPTNNSNGTSIAAVVGSVDAKFNSFPGSISVQQQKQETIQEMSKMCVERILAYQKSVGELPQTVLFYRDGVSLGQFNIILDEEVTAVKNSFSVVANRLGVKFDPKLTFITLLKSHNTRFFPLETNAKNEADKMVAVAAQENILPGSVVEKGVTSRSLYDFFLQSQQALQGTAIPGHYYVLYDENKWTPDELQKVTYNLCNVFGRATKSVRVVPPAYYADLLCERATCFVQGAVVGRNQNPVDAIKKAMGSGINKNVSGRMIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGA5</accession>
    <name>A0A1E3NGA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palA/RIM20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015711.1">
        <property type="nucleotide sequence ID" value="XM_019164735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGA5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGA5"/>
    <dbReference type="GeneID" id="30181422"/>
    <dbReference type="OrthoDB" id="64867at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="3">
        <location>
            <begin position="9"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="273"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="723" mass="83803" checksum="65313E0DEDF7A5BB" modified="2017-01-18" version="1">MTSLDRENDLLYLTPNVTSSVDFGPILGDVIDNQFHQPNELFKQDIQLITNLRSESFPSMIEDINLEVITKLKKYCDSVTALITIFPDEVVQFHWSNCVIKSLNEEKLYIVYQLASYYSILAVNESKRDEEGLKKSGIYFQYSAGCLEIVQQLLHHTSVQSFPLNSNKIEALKTVMLAQSQEIYLQKAKINQLKGSILLKLAIRISDFYNDAIQLIGQEVEQSNLPISFFQLKGLYYTCFSFNSYSAYCNRKKKYGEEISYLNQSLYLLMQVSEAEETQYSKLLQDAENLRMQILSKIETVKHENEMLHLQKIPPFDTLKPLPRAELVKCLAPKDMELSENSSTNNLFIGLIPLQVVIQVCEYQNRFKDFINCELVTPIEKLNQNIHELFTNLNVETEISKLTSNSDLPPALIQSREKLMRNGNLEKIANLISILQELKLKCRFKLDKIWQLLKDQTVEEEALAKYYGYDKWKLGVLEDDKVGAILINSFKIYENYMKQSEEGDRLICTQIEELKPFLEIYDDEKLLKSYVPETNVLVLNPSLQPMIDNLREVSMLLQRLEGERVNFLKKVKKKASGVRIIDQYRQKSCKHRLQNTSTHTDDLQNLLGEILKDEILKFNDEVEYLGGTRVAQDDLVSKFSACNLDFINLKDQLKISIKRKEALHVLDSTYQGFFEIVGNLIQGKDFYQNLLDNIDVKMNQLDNFLGKRSESRRFLQEKLESSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGN7</accession>
    <name>A0A1E3NGN7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016397.1">
        <property type="nucleotide sequence ID" value="XM_019159996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGN7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGN7"/>
    <dbReference type="GeneID" id="30176683"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03044">
        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000142">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="83"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="197" mass="22220" checksum="2D6A1F49DA627983" modified="2017-01-18" version="1">MSKGTLFIKPASPRSNWLDALVKTLNIDVDVVDYSKSEEYAKLFPLKKTPAFADSEGYELTEVLAIIEYLFSLASDKTLRGVTNKDQAQVMRWLSYVNQDMVNTWVDYLFVAKTEDAKKAASEQQAEQLAYINSELATRSWLAVDGYVTVADEYFFSWYAGFADVAGGITSAKYPYLTKWHETMKAKDVVAKSLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGZ6</accession>
    <name>A0A1E3NGZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_55112</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a key role in the late stages of endoplasmic reticulum to Golgi traffic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016507.1">
        <property type="nucleotide sequence ID" value="XM_019163181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGZ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGZ6"/>
    <dbReference type="GeneID" id="30179868"/>
    <dbReference type="OrthoDB" id="10254842at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000002">
        <property type="entry name" value="Trafficking protein particle complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017479">
        <property type="entry name" value="TRAPP_I_complex_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="11"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="254" mass="28691" checksum="9A600F67A594D629" modified="2017-01-18" version="1">MSSVSKVILQGTGYNPAIGPQSTDPKQQKPTPNQPATGVQQSSPLPSPEMIKKQVNVTSHIYDRNLYRTKHEISVSSLCFLFMQIISMNLKNSKSLLELERKLNNLGYTVGLKYLELSSLRVNFSNNISSTGKSNLSKRNIRLLESLQFIVSSIWTSLFDKQADSLEKSSEHENQYMIIDNNPVMTKYIGLPKEYENLNCESFVAGIIEGMLDISYYKCEVSAHTVPRDTFPNRTVYLIKLDDSVLAREARLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH05</accession>
    <name>A0A1E3NH05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_74195</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016548.1">
        <property type="nucleotide sequence ID" value="XM_019164703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH05"/>
    <dbReference type="STRING" id="763406.A0A1E3NH05"/>
    <dbReference type="GeneID" id="30181390"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF5">
        <property type="entry name" value="AFR253WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="11"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="259"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39524" checksum="86FC43935948B860" modified="2017-01-18" version="1">MDPLPTPNDNTGLSTAMFGGAISGIVQTALTYPLEYFKTISQIENKSIMLKEAVVPHDSMRPLFTGCLPLSLGMALKSSSRMFIFNQFSNFMADDGKTSAPRVVVAGLMTGVLESFWSIPFENIKVRMIENSMIRYGLKTTLDEEVQQPSKVNTDNATQSAKKSIRISNRPSSSNSVKEVAKEMIKHPRMKALEYYHHNPSYTFSNIVREMYETGGISAFTQGSTITLLRQGMNSMVWYSTYSSFQQLIDPSRDSITELELLAMGFASSMAVVGVTQPIDVIKTRMQTRDYRIVYKDLMTCIALIFFKEGVSKFWSGSVPRFVKVSCSSTVTLLTYEYVTKAVNTLSEIKPFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH43</accession>
    <name>A0A1E3NH43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17913</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016557.1">
        <property type="nucleotide sequence ID" value="XM_019161803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH43"/>
    <dbReference type="STRING" id="763406.A0A1E3NH43"/>
    <dbReference type="GeneID" id="30178490"/>
    <dbReference type="OrthoDB" id="28737at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000001">
        <property type="entry name" value="Heat shock protein HSP 90-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="677"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="694"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="100"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="704" mass="80605" checksum="4A44B3A614B856D8" modified="2017-01-18" version="1">MSKTESYEFQAEISQLMSLFINTVYSNKEIFLRELISNASDSLDKIRYQSLSDPKVLESEPELFIRITPKPEEKVLEIRDSGIGMTKAELVKNLGTIAKSGTKAFMEALTAGADVSMIGQFGVGFYSVFLVADKVQVISKSNDDEQYIWESNAGGKFTVTLDETNERIGRGSIIRIFLKEDQLEYLEEKRIKEVVKKHSEFVAYPIQLVVTKEVEKKVEEEEEKKEEETEGDDEKKPKVEEVKDEEDEKKEEKTVKELVTETEELNKTKPLWTRNPSEITQDEYNAFYKSISNDWEDPLAVKHFSVEGQLEFKAILFIPKRAPFDLFESKKKKSNIKLYVRRVFITDDADELIPEWLSFVRGVVDSEDLPLNLSREMLQQNKILKVIRKNIVKKCIETFNEISEDKEQFDKFYQAFSKNIKLGIHEDPQNRSSLAKLLRFNSTKSSEEMTSLSDYVTRMPEQQKNIYFITGESLKAVEKSPFLDALKAKGFEVLFLVDPIDEYAMNQLKEFEDKKLVDITKDFELEETEDEKKKREEEIKEFEPLTKALKDILGDQVEKVVVSHKLVDSPAAIRTAQFGWSANMERIMKAQALRDTSMSSYMSSKKIFEISPSSQIIKALKTKVEENGPDDRVVKNLTTLLYDTALLTSGFSLEEPTSFSKRVNSLIALGLNIEDDDEEADVDAAPSDKPAAAEEAESAMEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH49</accession>
    <name>A0A1E3NH49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Large ribosomal subunit protein mL46</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_60197</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrion-specific ribosomal protein mL46 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016570.1">
        <property type="nucleotide sequence ID" value="XM_019163415.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NH49"/>
    <dbReference type="GeneID" id="30180102"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04661">
        <property type="entry name" value="NUDIX_MRP_L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13280">
        <property type="entry name" value="PH_SIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040008">
        <property type="entry name" value="Ribosomal_mL46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021757">
        <property type="entry name" value="Ribosomal_mL46_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033650">
        <property type="entry name" value="Ribosomal_mL46_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042067">
        <property type="entry name" value="Sip3_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L46, MITOCHONDRIAL PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11788">
        <property type="entry name" value="MRP-L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="486"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="13">
        <location>
            <begin position="926"/>
            <end position="1089"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1302" mass="149440" checksum="0F4D36014163C4CC" modified="2017-01-18" version="1">MQHRIIRQASRSFATSAAALSNSTPIKASLVLSRAPIVTPDVPEFEKLFYNYQEELERRLMWTFPKWFYFKKGTVAEREFTEAQKYPVPAQRGVWFPQGVPDLKHGRDRRFKQEVILPKKHAEVEAPEEGSTDTAAALADLDDIGRAIKANSRVTQFDKANEQTSLERKLPRTLYLVVKESGAWRFPSFEVKPDASEPKGLHQIAEDGLRTLGGDKINTWSVSNSPAAVIKYDPKGQVANVKASDASALTTEFLMKSHIIAGKFDLQKASKNVSEYKWLVKEEIQQVRIIFESAMSLLRIDDLSFVNIGHILEVDIPHYLEIRRNFEKIQNKYDVISAKYMQLPKGYDPVKTREDALQLFEIRKQYIHMSMTLWICVKNLEGKISNGVTDFASSFRNSFETRNTSYGAKLSDAIGLTSIVEDIEQLVKSAELKQTSSMLLMKDLNRARVNSEDGAIKMYTPSPDTKDFDSSILNDKNIYLESEKNLLEKHGWVFIKSPKLNGRGDVWIKRWMFVKNGVFGFLSISQDGQYVQESDKIGVLLTTIKYMPSEDRKFCFQINSQLTNLVIQVETSVELKSWLTVFRNVTEVASAKKSEIALNRYSPCLDMLKLVPVVAKDLELIDVNPVDPQIEKTSKLIELQLSNMKFNLSINPPLKTSMTEKISMSHLYLSSTTIPSATTANFWGYVNWGLYFVLDEETKILVSNNTKTLSPRSVINLRYPDFYPESLRVADAELRSIFEVCIGNDECTLLRFNASWSPNSQQNIFCALYVTTESFYVYSHTYGLISILPISLSDFLEADVVEKANSTILKIYFVSGITIKMQLYDDDVYSIKTQFNFILKNRKSSHPKDLQHVVSQLSHIQQAYKKIRDERQMSITTSAIRPSNSDQLKLKGIKLEYPAGEDLNWIDNTTGISDLDSENRINYTSEMGLLLIKRFKVPAKALFHILFGDESFLLQCTLPLSSSIFKEVNSKHSLWRCDSKQRMTRVVWNPVFNVPCAMQTIERIVNNKYYNIVQETPHLRFVFGINRKISMRFIIYNLDSKSCKLMVYYSLGGGSNVLNWFSRKVIHQIMIFRMEALESRLTEAVSQIGNDNRKIASAIRTYGSITKYDSDEATKDELTFDDSVSFIPLQLFSSFYYEKVNFEIARLIYKGIRAFTNTIKTEITEMINPNTTDLSYAPSGTECYWHFLEHENLGSINQYSRIDKNDEIIDIYELHSLEGKREVSLAQKLTGLRIERNGLLTKLNLLNYIEKEFILKEWKNWVSSEISNCQKVQDTFPGSYEPIKSYCEEVEKEMLNLYENLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH81</accession>
    <name>A0A1E3NH81_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Urea amidolyase</fullName>
            <ecNumber evidence="11">3.5.1.54</ecNumber>
            <ecNumber evidence="12">6.3.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_17914</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>urea + hydrogencarbonate + ATP = urea-1-carboxylate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20896"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15832"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>urea-1-carboxylate + H2O + 3 H(+) = 2 NH4(+) + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15832"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (allophanate route): step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (allophanate route): step 2/2.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.54" evidence="11"/>
    <dbReference type="EC" id="6.3.4.6" evidence="12"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016559.1">
        <property type="nucleotide sequence ID" value="XM_019161804.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NH81"/>
    <dbReference type="GeneID" id="30178491"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004039">
        <property type="term" value="F:allophanate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004847">
        <property type="term" value="F:urea carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000010">
        <property type="entry name" value="Urea amidolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053844">
        <property type="entry name" value="AH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014085">
        <property type="entry name" value="Allophanate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014084">
        <property type="entry name" value="Urea_COase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02713">
        <property type="entry name" value="allophanate_hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006043">
        <property type="entry name" value="PRK08186.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02712">
        <property type="entry name" value="urea_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF128">
        <property type="entry name" value="UREA AMIDOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21986">
        <property type="entry name" value="AH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160467">
        <property type="entry name" value="PH0987 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="17">
        <location>
            <begin position="628"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="747"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="15">
        <location>
            <begin position="1754"/>
            <end position="1832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ45446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1832" mass="201469" checksum="3AB4809B2A68A64D" modified="2017-01-18" version="1">MNTIGWSVSDWVSFNRETSPFESLNTLKALLAHLQATHSSDPAWISLASEENLVHQWKILQSKSNKSDLKLYGVPIAVKDNIDALGFETTAACPSFAYQPTKDSTVVALLRDQGAIVIGKTNLDQFATGLVGTRSPYGITPCSFSDSHVSGGSSAGSASVVGRGLVPLALGTDTAGSGRVPAALNNLIGLKPTVGAFSTAGVVPACKSLDCPSIFALNLDDAQLAFNVCAKEDLSNCEYSRLLPDNPLKSYPPNLKIAVPIDFKGLWYGDEENPKIFQAAIENFTKLGAEIVPCDFSPLLDLAKCLYEGTWVAERYSAVKDFYATNPPEKDLDPTVTAIIKGGAKYDAATAYEYEYKRQGILNRVHESFKGIDVMLVPTCPLNPTIKQVNDEPVKINSIQGTWTNFCNLADFSALAIPAGFRSDGLPNGVTILGKAFEDYALLDLAKRFLKTLYPANDRRIGNVKGKVAGIKDNLNSSIPQPNLSKCIKLAVVGAHLENLPLHWQLEKVQATKLETTKTSSNYKLYALPITGPVQKPGLRRVESDGSKIEIEIYAVPTERFGDFIAMVPEPLGIGSVETEDGQWVKSFICEEFGYKQNGTVDITKFGGWRYYIAAQESEKAEQAGKKPFNKVLVANRGEIAVRIMKTLKKLGIKSVAVYSDPDEYAEHALIADEKIPLHGISAAETYINIEKMLKAVKDSGAEAVIPGYGFLSENADFADRLAAEGITWVGPSGDAIRKLGLKHSAREIAEKAGVPLVPGSGLVSSAAEAKEIAAKLEYPIMVKSTAGGGGIGLQKVDSEDDIERVFETVQHQGKSYFGDSGVFLERFVENARHVEIQMFGDGFGKAIAIGERDCSLQRRNQKIIEETPAPNLPEITRAKMRKAAEQLGASMNYKCAGTVEFIYDEKRDEFYFLEVNTRLQVEHPITEMVTGLDLVEWMLYIAADMAPNFDQEIIVQGASMEARLYAENPVKDFKPSPGQLIEVKFPEFARVDTWVKTGSIVSSEYDPTLAKIIVHGKDRADALSKLRKALDQTVVYGCITNVDYLRSVANSAMFEDAKMHTKILDTYDYKPNAFEITAPGAYTTVQDYPGRVGYWRIGVPPSGPMDAYSFRLANRIVGNHYKAPAIEITLNGPSILFHHETVISVTGGEVPLKLNNEEINMYEPIVVKRGDKLQIGKLTTGCRSYLAIRGGIDVTEYLGSRSTFALGNLGGYNGRVLKMGDVLFLGQPGLGANSLPAPICEPAAAPRSLIPTISTNKEWTVGVTCGPHGSPDFFKPESIEDFFSNQWKVHYNSNRFGVRLIGPKPKWARADGGEGGLHPSNAHDYVYSLGAINFTGDEPVILTCDGPSLGGFVCQAVVADAEMWKIGQVKPGDLITFVPISFDHALSLKEQQNMALSALEGEFSSITKALVKPENPVLSVFQANKHSPIVTYRQAGDRYILVEYGENIMDLNYSYRVHKLIEMVDRHQTVGIIEMSQGVRSVLIEYDGFAIHQKTLVETLVAYESEVAFTNKWTVPSRVVRLPMAFEDSKTLDAVKRYQETIRSDAPWLPNNVDFIANVNGIERSEVKDMLYSARFLVLGLGDVFLGAPCAVPLDPRHRFLGSKYNPSRTFTPNGTVGIGGMYMCIYTMESPGGYQLVGRTIPIWDKLSLGEYTKKFNNGKPWLLTPFDQVSFYPVSEEELEQMVEDSKHGKFEVDIIESVFDHTKYLSWIQANSESIEKFQETQDGEKLQEFKRLIQVANEELSKTGTKVEENVEEYPEDAEMIYSEYSGRFWKSLVKVGDEVKEGQSLVVIEAMKTEMVVSSTQSGKVLKVIHTNGEMVEAGDLVVVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHF6</accession>
    <name>A0A1E3NHF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016693.1">
        <property type="nucleotide sequence ID" value="XM_019164378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHF6"/>
    <dbReference type="STRING" id="763406.A0A1E3NHF6"/>
    <dbReference type="GeneID" id="30181065"/>
    <dbReference type="OrthoDB" id="1436450at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="179"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="515" mass="56403" checksum="36572CC14E1958D9" modified="2017-01-18" version="1">MSDGKDTKIFKSPRSLIKSPRFHDPITNLIFPGSPSIKTSHDVVSQEQVLDEVVDIIVAEEKNVWITGLILLSFSVLCWVSSVQLLNNVMKKTDFDHPLLAAYLNGGLFILFGIRPLIREWWSTLTRLGNLSIYNIGAEPENAAETESCLPNGNSPPYIIESDANSNFSQLETPKVQLSHHEIIKVSACSALLYFTCCFLGSSALKYTSASNQTVLATSSSVFSLIIGVALKFEKFTVGKVISVFCSMGGIALITFSTNASNYNILSALVSPDKFGDLLAICGACAFSGFLAILRLKLGEQTDSERDSLVYGYLGLLTLVLGFPVLLVFDFFNWEKLSLPENHIILLMLFLSSFLNCLSDYCGSCASLITSPLSVSLSLSAAIPITMLIDSYFYGGSNFSIQYLTGVVLIIASFVFTNVSNKNEIVENAIENAIEEAINYDEQLSFILSPRLLATDSNHTATISDSNHYSNSAASDIPGLSIDHSILDSMEELPHQRLVVTGGQNHKYFFREITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHG3</accession>
    <name>A0A1E3NHG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Ribokinase</fullName>
            <shortName evidence="12">RK</shortName>
            <ecNumber evidence="2 12">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RBK1</name>
        <name evidence="15" type="ORF">PICMEDRAFT_36720</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose + ATP = D-ribose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="2 12"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015896.1">
        <property type="nucleotide sequence ID" value="XM_019163029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHG3"/>
    <dbReference type="GeneID" id="30179716"/>
    <dbReference type="OrthoDB" id="415590at2759"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="Ribokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="14">
        <location>
            <begin position="3"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="38"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="280"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="316"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="370" mass="40188" checksum="5163227E9145D117" modified="2017-01-18" version="1">MPVTVFGSLNQDLVTHTVKFPGPGETVQGVSFERHLGGKGFNGAVAVAKLKAPTDRFTVRLLGCLGDDDAVANDFIAYLKANNVSTSLLKKVKNTATGTASIVVQDNYAGENRIIVVPGANAHLHPTPAELERCFNASNHPDLNTSIDNPALPSHADDLAHFVVLQNEIPNPTAIIHTLASSYPNVRIFYNPSPLPARKAGYNQDFLSALHESHYLVVNEHELAGLVKNFHPNPDREPLTLLSNTSNFEPHSDYADLVNLNIKLMTKLRTLLTKPSLIVTLGPAGILYSEEGKFNYAYILAEEVDSDDIVDTTGAGDTFLGAVVTSVYRNETLESAVKFAARASAETIKSRGAAESMPYWKDVEKRGWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHG6</accession>
    <name>A0A1E3NHG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016703.1">
        <property type="nucleotide sequence ID" value="XM_019164381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHG6"/>
    <dbReference type="STRING" id="763406.A0A1E3NHG6"/>
    <dbReference type="GeneID" id="30181068"/>
    <dbReference type="OrthoDB" id="5300823at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052841">
        <property type="entry name" value="PMP_oxidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28040">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE YLR456W HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28040:SF1">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE YLR456W HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="181" mass="20102" checksum="5B3CE1FF6B14228F" modified="2017-01-18" version="1">MTGGPLTPSITNLLSHSSLLHLATCSDNIPHVSLMNYTFIEPNEEDSSSNIALLKNSRNLVLVATPKNTQKYENLQKNGKCSILVHDWVANGNQVNENSVLKLLYSINQSEVGDMSVTLDGHVVKFLLDSTSEEYEFFKNLHLKKNPVAKAFIEGDNVALILIQIDESKVSDSNNKVEKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHH2</accession>
    <name>A0A1E3NHH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_178910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016121.1">
        <property type="nucleotide sequence ID" value="XM_019161795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHH2"/>
    <dbReference type="GeneID" id="30178482"/>
    <dbReference type="OrthoDB" id="62495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="295" mass="34277" checksum="932D7019B2163791" modified="2017-01-18" version="1">MNSEEFNNFVRITTELTCKSTRSLTFQNYVEERLNAIDYNEFLRASNQGERPRFYIRLKNPERKNTNERGLKVCLFNDNLFEEKVVSPGTTIIKFAPMLKVIGFTNDNCSECGKHLIDIGDKGKEESENKHQVENGLTQAKEFVKNEGIVKCKNCNFRVFCCQECKLKAMLDWHDLECDFIAHIQKELITTNNGIKVDTETDQAIEKIILIIITTFRLLISLVRNDEKLFEIVINMSDHLKLYQKYVEKGEVDSPEHEAIFRETQHTFGPLIASYFEEKKTKSNRLKSNQLSLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI03</accession>
    <name>A0A1E3NI03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylation factor GTPase-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_17031</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase-activating protein for the ADP ribosylation factor family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016864.1">
        <property type="nucleotide sequence ID" value="XM_019161436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI03"/>
    <dbReference type="STRING" id="763406.A0A1E3NI03"/>
    <dbReference type="GeneID" id="30178123"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF160">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="431"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="602"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="837"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="855"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="881"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="903"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="933" mass="105058" checksum="8FCE8FB370680165" modified="2017-01-18" version="1">MADMIIGALNYPSLSEFLTSISFLGSTSENSIQLDIVSNSISSTSNVELIRLSSHKEDGIDFVLKIPFIYDRLLLKFVKTLNLESMKCSLTALKTSSTFHVNSHDIKLPLNNSYTDLLTLKIYYENELVYELSFYREPLSIVTNLPRTSPKEVIPTTSSSQFKDSQSKSTHDKTPSKEIASENPFPIPMADGPLLRETLNSYEQLAPYVLRHLQQTMENINAMDQNLKGLESSRFQLIDTLKEFRNEYLSTLPKSDLVYSKFEFLHSNSSSSEDSNHSFLKALHQSTSETKIDFSALRTLQNFSSSKKNFEEESKKYYDWLSKLMSSGKSKDEKLLIKMKNFAVAQMNYFNFLYDTVTPMLLSLIQPTSPFCKNYWRLRPLRDQAVKKVQNCSTFDEFSTLIRRYSKVTNCKNSLLLSDKNSCYETDKFDTPLKAGLLFVFGGQGKSGWHKQWLVLCNGKLFEYMDWRKGADLRNSPIDISLCNIKLLDANDHNTIDIGSRKNCFRVINAKGVEHVFQAFTSEDANEWVKALFEAGQMIAYAKQNNEESKRESSAKKLSISKHTVNTSENQNKYKAQNDLSADSSSLRKEPLRVRTVSSVSLSLLHIVQHNDPSNIYCADCGSTEQVEWISLNLLVVFCIQCSSSHRALGTSISKVRSLMLDSFVGESRALVHHINNSKMNSIYEANLQPNQKPVPSSRNEERLDFITKKYMKRAFINNSIRSNALQLLLEGVRDDDVVKVLEGIAGGADVNRKIFYSINQSGLSIISMTDSKEQNYAKVSFLEYALLHPSILDGREVFDVAELLALNGCDAGTQIRNESRVNENAKKWWQDRIDRMNGGALAPSPPPQRPLQLGNKSGTATLASPNGPTGAGHMIPVKQSGYNSNSRVSISGSRPSLSITGSYKKPSTSSKSKIKSPKEGFNLFKKKIKSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI68</accession>
    <name>A0A1E3NI68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_73325</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016940.1">
        <property type="nucleotide sequence ID" value="XM_019164449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI68"/>
    <dbReference type="STRING" id="763406.A0A1E3NI68"/>
    <dbReference type="GeneID" id="30181136"/>
    <dbReference type="OrthoDB" id="19039at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="618" mass="70198" checksum="D53ACD62736384B4" modified="2017-01-18" version="1">MKYSVKVQVVLDVVLVVSTTFMVLLAPYTKVEESFNVQALHDFINVGVDEIMLFDHVTFPGSVKRTCLGILFLSLPSIFFRDTMKKWAGILVSLAILLHRTLPTSIGMLSYGGIEEMFTMTKLPQLIVTRFLLAIFTCVSLIHLRRSVLYSCSQNSRLIGLWFSLLLYPLPHIIFYSSRFLPNFLCFPLTNLGIALFISGDITRSLTILVFTGIVFRFEVLIFTGILIVLCISGFFRDGNSLISIREAIVSVGVSGIMSIFLCSRVDSYFWDVKFVIPEFESFVFNVIGGNSSDWGVESPVAYFTKYLPKLFAAGFGVVLILSLVFFILSLLNLKMFYWKKERYRFEVDYVNYGVGTITTLMWAALIYICVLSVNGHKEWRFLIYTVPILCIGAASTAEWMMSKSKILKRLMITLIILCYTFSLASSFFFGYISSWNYPGGDACQKLNLRLIDMYGSSQGTMLKPLIVHWDVGTCMNGGSLFTQISDNKVLGDGWLEEEGGREPKFWVIYDKNEDSEVLSEIVDDFDYWIQYDDEEIIPLSEGYQWLLVDVSEGFTGVDYKFLVDTLKNPRKIGLQLLLAIKTGDTSWFKNVADSVIKKKVRGRIWERSAADKVFNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIH2</accession>
    <name>A0A1E3NIH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016491.1">
        <property type="nucleotide sequence ID" value="XM_019161778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIH2"/>
    <dbReference type="STRING" id="763406.A0A1E3NIH2"/>
    <dbReference type="GeneID" id="30178465"/>
    <dbReference type="OrthoDB" id="10253329at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="492" mass="55880" checksum="F49116B85250590A" modified="2017-01-18" version="1">MTPADETKLIARTATESKAGTSGSRIRNKNRKKNSKKSEECSSSENIDSTGSPVETQTNLRGTIGVAKSAKVESKRKSPPIKALFKELEKLLRFVNPITLNGLPVKNLTREPIPFLKNVIEDETNKDEIFMTFLMKPSDPDFPFDLGILSVTLCIPQSYPNKLPSPTIMVLNDDIPRGYGLNIEFGFKQIVSTVLENRRSKKQGKWSVKGKGSGKGGNTPEEDEKDEGRTTEGLLNIEVVGGNDLTGMIKTLDKYLEKFLSMEKKDTIKLVKVMNSKKDSEDKKLKEKTDDGKAANYESGMNSTTPSPRIERYRERTEELEQLRQRLRDNSVTVLTNNVHGTTYKFVLYFKSDDLTVEFGESDEVTIEKLFIKLFVPKEYLYHPKKGIKLSIDMSNNYNIGLVNSIKDTNSRLIFGKLINNIGKNYDLFAQDICQINCQNPSRDSLLFWTITSQLNFFVHNIQKFMSERVDFQNWYEANKTMNFEASSEPNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIS1</accession>
    <name>A0A1E3NIS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_148630</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017124.1">
        <property type="nucleotide sequence ID" value="XM_019160573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NIS1"/>
    <dbReference type="GeneID" id="30177260"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="46"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="39996" checksum="2E7BA70B722B786A" modified="2017-01-18" version="1">MLSQTLSAARHSAFKSQPLRLSLRSLATQAPTIPKTQKGVIFYENGGPLEYKEIPVPTPKPNEILINVKYSGVCHTDLHAWKGDWPLATKLPLVGGHEGAGVVVAKGANVTNFEIGDLAGIKWLNGSCMGCEFCQQGAEPNCPQADLSGYTHDGSFQEYATADAIQAAHIPQGSDLANVAPILCAGITVYKALKTAELRPGQWVAISGAGGGLGSLAVQYAKAMGLRVLGIDGGAEKGDFVKSLGGEVYIDFTKSKDVVKDIQDATNGGPHGVINVSVSPAAISQSCQYVRTLGKVVLVGLPANAVCESPVFEHVVKSIQIRGSYVGNREDTAEALDFFSRGLVKSPIKVVGLSELPNIYKLMEQGKILGRYVVDTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIW1</accession>
    <name>A0A1E3NIW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_25537</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016631.1">
        <property type="nucleotide sequence ID" value="XM_019162399.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NIW1"/>
    <dbReference type="GeneID" id="30179086"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903452">
        <property type="term" value="P:positive regulation of G1 to G0 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901992">
        <property type="term" value="P:positive regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001008">
        <property type="entry name" value="Serine/threonine-protein kinase cek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="615"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="984"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="1287"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="251"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="511"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="998"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1114"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="575"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1022"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1032"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1059"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1120"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1137"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1416" mass="157326" checksum="8205ACF1E7634CD1" modified="2017-01-18" version="1" fragment="single">LELDLDCNIKFVSKSWESIVGTKIAKIVNRPIHDIIAGDEEDKNVFTKATSIMTIDDETYRIRFMTETNLKNSTSPNTNSANDENETETINSKTSNDNSDNDSLSIHSDSSTITTDGGFIELEAQGILIHDKNEIPSHSMWIVKPWIPINDVTLELPEELISTIGVFGVNLLESYMLYLTDLEITDENDLPPPSLELCRICEEKVPNWWLEKHSELCLVEHRVEDMVYIKQEELQDHKKLLQNILETLHRRQPPSPAISSPSSSSSSPGSPPVLSPSSSSSSITVSPISSDSTSSSNSFVLVSDYKGMPIPLGPQSSGTLPFPARRKSAGTLFPQIRFPVKNIENLIAYCDEALKINPGEVRSENRESVELFEIAYSPDSASALKTLNELRLPSSSDPAIQQLTEDTKVLVADKLETLERYAHILQYVDRITKETSDLVIQAVDNTIRKIKEHVFCVSESESENDSSSMRSTRDVSVHSVHSVHSVLDSGSSTPRNSAALIPRRSTPVITVLSEDSGKFKKHPSTHSLSTPRRPISPGYPMPLSSVQKNSRRSEASVNSPFTSPLLQSAETTKISNTPTSSLSTNPNSAYNEKTPLSPLLVPTTVKHNLPSIKDYEIIKPVSKSAFGSVFLAKRKLTGDYVAIKVLKKSDMIAKNQVTNVKAERAIMMAQADSKHVVQLIASFQSTHYLYLVMEYLNGGDLATLLHNMGSLPDVWAKRYIAEVIVGVDDLHTKGIVHRDLKPDNLLIDHSGHVKLTDFGLSRMGLVNRQKAANSTMNESESNTNTLLDKKLLTVEPFSLNSSKPQDDITPERESIKEYFPPVTNELKYHDSASSGPISRLQHEYSTRARRLSTASHSLETPKTYALFDPSQSTQARKFVGTPDYLAPETVAGRGQDTTSDWWSIGCILFEFLFGYPPFSDETPEKVFNNILYEEIQWPQLSEEEFRRYCSDSARDLIEKLLVKDPTKRLGVGGSAEIMDHSYFEGINWDTLFDEEASFVPEPDHPESTDYFDQRGATMNSFPEDEEGEEENTSQYFNASTSYEMKRDSSSSSTIDSPRQSLSKNSSQLLSFRERRSSRLNEAGSNSEFGSFQFRNLMVLEKQNKDAINRLKSEHLEHRNSSSSSAYAANSSVPATPGTPSLSATSASRGRAQQVLTPHKRSLSPNPQLITGTSKSPVLSFIQSPVLKNNISALDSDVASAHNKPFPILRSTSGSGSCNGTRSPAIHILTKSFTRTLSDFSPSSSDNEEKNVILSKVNRRKFGRLRNRASSSSQYDEPSLTSLLPKLTVLVFEPIPIHRFSITRDLKELGCIVFSCASGSELIKLSSGNIEFDIIFTSSESQRLNSIDLVKLIRHTNSLNTNSTIVALTSYSRDTQAFGIYDYVIEYPINKKKLKEVIKSVQKARVDTEEAIVTDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIW4</accession>
    <name>A0A1E3NIW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_23684</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017163.1">
        <property type="nucleotide sequence ID" value="XM_019162318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIW4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIW4"/>
    <dbReference type="GeneID" id="30179005"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="217" mass="25205" checksum="7A0C54D2E2A46D10" modified="2017-01-18" version="1" fragment="single">LLAKELKELSRNPTYHISLDNDSIFDWTLGLYIVNPESPWHHAYLQAKMHFPLNYPFSPPSFKFSPSIFHPNVYSDGRVCISILHEAGNVYQDEPSNENWSPAQCVESIIMSIVSILDDPNIMSPANIDASNLWRDNKDVYNDSVKKFAERTHIYIPEDFDIPSDKVVVGTTAQDEEDDWYFEDESEGEEEEEEEEDQMSYEESEAEMSEDYEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIZ9</accession>
    <name>A0A1E3NIZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_16859</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016671.1">
        <property type="nucleotide sequence ID" value="XM_019161368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NIZ9"/>
    <dbReference type="GeneID" id="30178055"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="187"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="318" mass="37273" checksum="0BC4F852B7EAA878" modified="2017-01-18" version="1">MVNLIINPLSKKLKKINCDTTPNTRLSAIIEAYAKKNSVSDPNRIKFSYLAEENDKEKDKKKRLPLKNYLTLEENGLDFAQASTLNVYAKDVGPQIGWRTVYFLEYLGPMIIHSLFYYGYYSASDMTYTQVLAYNLTLLHYLKREYETLFIHTFSADTMPFAFLFRNSGHYWINSLLIAFSVYSPQSVVYSNKFWSVIHHVEDRSLDELKWFGGAMVLFEICNLYCHIILRRLRSDGTRAHKIPYGFAFKFVSFPNYLFEFLSWTVFAVMTNNWSSYVFLVAGTGTMMMWAKQKHAKYKKTFGDKYPKNRKAMIPFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ86</accession>
    <name>A0A1E3NJ86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_59711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017284.1">
        <property type="nucleotide sequence ID" value="XM_019163383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ86"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ86"/>
    <dbReference type="GeneID" id="30180070"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="61"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="306" mass="33983" checksum="E5ED20ECB5E395EA" modified="2017-01-18" version="1">MSQTVVTSTTASGVPQKVAPVSSRGYFMTQRGASNMAMGNGNPHKIPKFDDPYKQREWMLEHMAGAFRVFGRKDFAEGTAGHISVRDPVDPTTFWINPLGIHFSMVTAADMIHIDEDGNIIGGNTSGTINAAGFAIHSALHKARPNINAACHTHSVWGRAFSCFGKPLEMLNQDSCYFYKSHAVYEDFGGTAVEKEEGERIAKCAGETGRALILQNHGLLTVGETVDEAAYLFTLLERTCKIQMMVNAAETEFYRKQLIGEDEAAYTNFISADPESMYMAFQPELNLEKFRDDSFMSYTKHPRHDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM3</accession>
    <name>A0A1E3NJM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secondary thiamine-phosphate synthase enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017389.1">
        <property type="nucleotide sequence ID" value="XM_019164173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM3"/>
    <dbReference type="GeneID" id="30180860"/>
    <dbReference type="OrthoDB" id="10255963at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16172" checksum="0C0F902C98C2BC90" modified="2017-01-18" version="1">MSTAWEQHKFSFSPRARGCYLVTDEVVRNMPSLKEYTVGTVNLFLQHTSAAISLNENCDPDVRTDMTTALNNIVPDDEGEVFVHTDEGPDDMSGHAKSSVVGVSLTIPISNGRLALGTWQGIYLMEFRNYRHTRHCLATIQGLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJQ3</accession>
    <name>A0A1E3NJQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_33858</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Vacuole membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017485.1">
        <property type="nucleotide sequence ID" value="XM_019162849.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NJQ3"/>
    <dbReference type="GeneID" id="30179536"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016242">
        <property type="term" value="P:negative regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905356">
        <property type="term" value="P:regulation of snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05169">
        <property type="entry name" value="PIKKc_TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000020">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.150:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000371">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.150">
        <property type="entry name" value="FKBP12-rapamycin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009076">
        <property type="entry name" value="FRB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036738">
        <property type="entry name" value="FRB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024585">
        <property type="entry name" value="mTOR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026683">
        <property type="entry name" value="TOR_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08771">
        <property type="entry name" value="FRB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11865">
        <property type="entry name" value="mTOR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01346">
        <property type="entry name" value="DUF3385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01345">
        <property type="entry name" value="Rapamycin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47212">
        <property type="entry name" value="FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="996"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="FAT" evidence="17">
        <location>
            <begin position="1251"/>
            <end position="1831"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="2005"/>
            <end position="2322"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="18">
        <location>
            <begin position="2352"/>
            <end position="2384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ46372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2384" mass="271815" checksum="BE13D72714834BD5" modified="2017-01-18" version="1">MQNFTLDQIFQELKSNDEIRRLKAAKNLRVHFISISRDSTSTEQMTVYNNYINKKIFDLTNSSKTNEQLGGIDAINSLIELLSPDENSNMITRYANYLRRLLSSNDINVMRAATATIGKLAIPGVSTTADFVEFEVKRSLEWLITEKIEGKRHAAILIISSLAENSPALLYQYVKQIFNNIWIGLRDSKSSIREDSAVCLRHCLDIVYERDNDLRNYWFSKLYSEAFSILNSPPEFIHGSLLCFRELVNQGTSVLGSKIDEIYETIMRVKDYKSVDVRREVTTIIPQMARYDKIKFVDRYMHRVLLYYISQLKVGKDKVMILLSIGDIATEAKNNMVNYLNGIVLESVRDGLSQSSIKVQRELSPACCYCLAKLVLALGPPMTKFINAYQILRLLLKSPINDNVLSVLKIFSKHLPSLEPIINDRLINNVSCCLSGYEFRHPGSPDFERLFDPDLAYNYRYDMLLKEDIPASKYFEDPDVTIILQGLKTLRCFAFKNYDLTEFVQYAITHYISHQNPEIRLQASVTCAELYRRSSICYERSAHSLKSVDFVLEKLLTTCITDPVAEIRLEVIKSLDDKFDPHLSQAENVKLLFMTLNDEDFEIRKQSYRVVGRLTFFNPAFIVPPLRKILIQLITNLEYGSHTTRIKEESNVLLGILISSTDDLTKSYTKQIMNALLPQAADISPVVSASAITTIGCLADVSCEDIMPFVPQIMPILIDSFQEQNSGIKRDASLKTLGQIAGSAGYVIQPLLDYPQLLGLLVNILRSESSTIVRRDTIRLLGILGALDPYKHREVERKGHDAETVARQNAPPIDMELLMKGKSPTNDDYFPTVVIRTLIKILKDNSLAAQHLAVTQTIMNIFSGLGMKCVPFLDQVIPAFEIVMHNCPISMLNTYFQQICDLIDIVKLNIRSYLPQIFKLIQEFFPNVKLQSKVICVIERVSKALEDEFKLYMLDLINIFLNVLEKDLSPGNMSTLRVLKAFVIFGSNSEPYIYMIIPSLIKLFEYASVNVRKAAIECVGRLSRTIPLNDYASEIIQPLLRLLSTYGGTGLRTVAMTTICSLLLQMGSDFKVFIPVVSSVLIKSKLQFPLYDQFVDRLNKGRQLPSGVVLYPDLETTTSDNIQAEQPQKKLSVNPQTLRLVWDCSSKRTREDWQEWMRKLSIALLKESPSQALRACSTLASVYPQLAKDLFNCAFASCWNELHIQYQGELAQALCIALSSVNSSPEIHQALLNLTEFLEHDEKSLPIRIQTLGQYAQRSHAYAKALHYKELEFIQEPSIPTIESLISINNQLQQSDAAIGILKYAQEHHGLQLKETWYEKLQRWDDALHAYNEREKEEPNSTEITMGKMRCLHALGEWETLSMLAREKWDTSSADIKRSIAPLAAAAAWGLRQWERMDTYIAVMKKDSPDKAFFNAILSLHNNNFDEASLQINKARDLLATEVTALVSESYNRAYGVVVRVQMLAELEEIIKYKCLPQGSDKRIHIIDTWNKRLLGCQKNVDIWQRMLKARALVVKPKQDMEIWIKFANLCRKSGRLRLAEKSLNSLLDEGSAGKPGNKASPHVVYAQLKYMWSRGQKAEALNHLVDFTSKLSRDLGLNENEAITQPLPTNIPGITDDIEKFTKLLARCYLKQGEWKIAMDDTWIEKEAPSILGSFLLATHFDPKWYKAWHNWALANFEVISPQSKHRQNYIVNGNSNASGNGISENNGGDNKMDVQHHNINMNMILRYVVPAVKGFFHSIALSFSNPLQDTLRLLTLWLDFGGVEEVATAMQEGLQMVKVDTWLDVIPQLISHIHQPDPVVSHSLLGLLSDLGRAHPQALVYPLTVAIKSESVSRQKAALTIIDKMRAHSSILVDQADMVSNELIRVSVLWHEMWYEALEDASRAYFNEHDVEKMFSILDPLNELIQKGPQTIREISFTNAFGKDLSDAHGWLHQFKRTKDVTYITQAWDLYYTIFRRISKQLPLLQNLDLQHVSPKLLNAHDLELAIPGTYEAGKEVIRIMKFDPIFIVITSKQRPRKLHIHGSDGKTYTFVLKAHEDIRQDSLVMQLFGLVNTLLANDPECFKRHVDIQKYAAIPLSPSSGLLGWVPNSDTFHVLIKEYRDPRKIYLDVEHRVMLQMSPDYDNLTLLEKVEVFTYALDITRGQDLYKVLWFKSKSSESWLDRRTTYTRSLAVMSMVGYILGLGDRHPSNLMMDRITGKVVHIDFGDCFEAAIMRDKYPEKVPFRLTRMLSYAMEVSGIEGSFRITSENVMKVLRDNKDSLMAILEAFAYDPLINWGFDFPIQRVIEQAGLPIKLQEGNYVELLRNGQITEEEAAVMAMRYKAGVRDARAACVLKRINDKLTGNDFKRFKGLDVPTQVDKLIQQATSVENLCQHYIGWCAFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJX5</accession>
    <name>A0A1E3NJX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72512</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017557.1">
        <property type="nucleotide sequence ID" value="XM_019164215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NJX5"/>
    <dbReference type="GeneID" id="30180902"/>
    <dbReference type="OrthoDB" id="439710at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070146">
        <property type="term" value="P:mitochondrial aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001750">
        <property type="entry name" value="PRK00476.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="178"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="659" mass="75303" checksum="45BA85330D925203" modified="2017-01-18" version="1">MRSSFGYCLFRRRFHSLSVVQAALPDKLRTIDKFDFKHFTHTIGEVNNAYTDLLDSNVTLNGWVDGSPRKISKNLVFAKFRDFNGDFTQIVTKSEDWSKRLRTLKPEDALSVTGKICIKKQKSQEERETWELSIEKFQILNDSNEKATQLDSLKETPNQFPAEYRYLQLRLPYYQRALKMRAHAARVARSVLDEMNFTEIGTPLLFKSTPEGAKEFLVPTRKTGFFYALPQSPQQYKQLLMASGFKGYYQIAKCFRDEDLRADRQPEFTQIDMEMSFAKSEDIQNVVQKLVSKIWRKVKEVPLYRVDWETGKLVELKDCETFPKLDYETALSKFGIDKPDLRSTITIKEVSQFFASVLKEDSFGVLEGCVLKGGLNAAKKLPKQLFDVAEYKDRKPYIFKIKNAEELGSWISKIPVKLKDGTSPSELNTYLGLDVGDIVAIGDRACLTYENPTPLGRFRQLAIQYFPTHWRREISEGSTPQKLEDIFVVSWLVDFPLFSPIELESKNGYPAYDRTKYESTHHPFTMAKLDDYEYLATEPLRVKGDHYDLVINGVEVGGGSRRVHDAELQKYIFSEILCIKNHMQLFGHLIHALDSGCPPHAGLAIGFDRMCSMLVGSSNIRDVVAFPKTQTGSDPVVESPSEVPTKTLNIYGIEVKGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJY7</accession>
    <name>A0A1E3NJY7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017543.1">
        <property type="nucleotide sequence ID" value="XM_019161162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJY7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJY7"/>
    <dbReference type="GeneID" id="30177849"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000005">
        <property type="entry name" value="Sui1 translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="5">
        <location>
            <begin position="27"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="109" mass="12496" checksum="74E2EE3E5290EE9C" modified="2017-01-18" version="1">MSNIENLKSFDPFADTGDDETELTNYIHIRIQQRNGRKTLTTVQGLPAEYDSKKILKVIKKEFACNGNIVKDDELGEVIQLQGDQRLKIMEFMVQTLGLKKKNIKIHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK70</accession>
    <name>A0A1E3NK70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_59370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016846.1">
        <property type="nucleotide sequence ID" value="XM_019163361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK70"/>
    <dbReference type="STRING" id="763406.A0A1E3NK70"/>
    <dbReference type="GeneID" id="30180048"/>
    <dbReference type="OrthoDB" id="364348at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15840">
        <property type="entry name" value="SNARE_Qa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF38">
        <property type="entry name" value="LD27581P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14523">
        <property type="entry name" value="Syntaxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="226"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="318" mass="35398" checksum="2CE50C5F2A3CF520" modified="2017-01-18" version="1">MSFATYRKNGDSAGKKRAEDLEEGEQDSVSATLKQISATLAEFISLVSSISKQEAFLGTQRDTPALRQGVNGSIANCQRVHKRLLENIDYLERTVLKSADGEDGSRGSKELMTVKFSKDLLRQQVQDVYKNYQVVVRSYNEKLHSALVQEQYEKTLERIREESNAATYKTALLTPSGEGVHGDGYSSVSEEAATGAHAQTQTQTQTQKQTQKQASELSEASLQYHSDLIHQRDNAITSISQGVQDINKIFKDLDHLVSQQGEQVDSIENNMTNYATNNQLASHELVKADNYQKKKRKWTCVLLAALVIVLLIVLALIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK98</accession>
    <name>A0A1E3NK98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_24598</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017662.1">
        <property type="nucleotide sequence ID" value="XM_019162358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK98"/>
    <dbReference type="STRING" id="763406.A0A1E3NK98"/>
    <dbReference type="GeneID" id="30179045"/>
    <dbReference type="OrthoDB" id="869189at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="108" mass="12244" checksum="26C683BB66B33A15" modified="2017-01-18" version="1" fragment="single">AFRRFLNSETGPKTIHFWAPFMKWALVLAGANEMTRPVEKVSATQQLSLFATGAIWTRWAMVIKPKNVLLASVNFFLGTVSIVQLARIFEWRVNTIGDTPTNAIKYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKI5</accession>
    <name>A0A1E3NKI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase DBP9</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent RNA helicase dbp9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_17106</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016956.1">
        <property type="nucleotide sequence ID" value="XM_019161467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKI5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKI5"/>
    <dbReference type="GeneID" id="30178154"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033677">
        <property type="term" value="F:DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="49"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="243"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="555"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="15">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="561"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="578"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ45843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="588" mass="67327" checksum="F597B39702CAE270" modified="2017-01-18" version="1">MSDSKVAVSKDYVDDTITFDSFNLDPRLIQAINKLGFEHPTLIQSQSIKLSLQQKKDIIAKASTGSGKTAAYCIPIIQSILVQNESKTADSKNEIQSVILVPTKELAKQVSNFLKELTIYCGKHVKVVNLNDIENNQLLTAVLNDRPEILVSTPSKLLNVLEEHSDVSLMELKYFVIDEVDLMLSYGYDEDLEKLSNFLNLKQHMQVFMMSATLNEDINTLKQKFCNKAAILKLQDLDNQNKRLLQYYVKTSEYDKFLLIYVILKLNLIKGKILVFVNNLDRGYKLKLFLQNFGIRSCILNSELPINSRLHILEEFNKNVYNLLIATDESNEYDDDTEAEVGESTTDASMLDHGKDVEDNKSAKKLGKKSNDAKDYSTSRGVDFKNVACVLNFDLSSSSKAYIHRVGRTARGGKSGMALSFVVFKNEWGKHKASSLPSARKDEKVLAKIERTQKKLGYEIEPYQFDNKQLDNFRYRMDDSFRSVTQTAVREARLKEIKMELMTSEKLKRHFEENPQDFETLRHDKALSKVRSDVNLKRVPDYLLPPGARQDVKKLGFVPFTKHKKRQQQGKRRSGGKKKVDVLKKYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKN6</accession>
    <name>A0A1E3NKN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017817.1">
        <property type="nucleotide sequence ID" value="XM_019164285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKN6"/>
    <dbReference type="STRING" id="763406.A0A1E3NKN6"/>
    <dbReference type="GeneID" id="30180972"/>
    <dbReference type="OrthoDB" id="420076at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF792">
        <property type="entry name" value="GM14286P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="164"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="580" mass="64640" checksum="E7A6627E2ED3446F" modified="2017-01-18" version="1">MLKIGKKQHSSLSHSVKGRLSLERGNYFGSNILTCSIHTLKRPVVYSHMRRGVNIALFSGLILGVSYYAMTNNALRADSNGASMSTITNPFSSNAKTPGEQHFFSTTTTNSLNSGGPSAQQHKQGIPVFTDKEVNKRLRQYEESYYVNRGKGVYRYDISQLPSNNPIEDDRAEKIVQVPVQDVYSNNEKVEADWHFWTVFDGHAGWNTSAYLRDNLIDYVISELNGAYKVSEKNLRLIPSNEVIDKAIKQGFVKLDDEIVNKNVQKLLDSSNKNAAAELLMPALSGSCALMSFYDTHSKILKVAVTGDSRALLGSLNEESHNWTVEVLSVDQTGSNPTEVAKLLSEHPNEPNVVRNGRILGSLEPSRAFGDARYKWAKDMQAKVYNQFFGRQSPANLKTPPYVTAEPIITTHEVNPTKNDFMVMASDGLYEMLSNEEIVGLVVKWMESKKMVKPKRGIFSSVWTSSKDKKLPEVVDVSDKAGNPKQRYRRTSNMTGYLLEDENVATHLIRNALSNGGKKDDVNLLVSIPSPLSRRYRDDLTVTVVFFDDHEKITGSANGMLEINRAATAGGLDENVRSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKP8</accession>
    <name>A0A1E3NKP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stress response protein NST1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017827.1">
        <property type="nucleotide sequence ID" value="XM_019161252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKP8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKP8"/>
    <dbReference type="GeneID" id="30177939"/>
    <dbReference type="OrthoDB" id="21629at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="715"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="888"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1176"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="715"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="888"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1191"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1227" mass="140138" checksum="E00A1D71CE4962CA" modified="2017-01-18" version="1">MTQNYIAGEDIHFEYEDSKNNKIDKAYVANRDMTVQVELDNSHEAYPVSRVIKQEANGNVVVQEVVDDHKFHSFHRSQRYNEQGEIGEEDEVGEDYNDDEYDYLHSICAYCQNDHRCDHDMQMEEEEEEEEEEEEYEYEEEEEEEEEEEEDYNEEEDEEDDDEYNDHDCQCPHHHLHYSHHHHHHHSQHYTKHEHNQYDNHHASHRHCKDTSGVPRSSLLEKQKRIIKEQIQRPVSGNFLWDFEFPEDIPDMIEFWIGLPYERKREIANIDFLKELDQIHDHQKPQGSCRLCGNRKSSIEKELEKLYNGYYNVRKLATESLDECDLNIETINTIFFIVEEEKIIEQEESTDALERELLSMADDLVKNNGENFISLIEQLDGEAKDSRISNASSVSTANDENNSDIRKTHGPEINKNDNDLLQKGENILYNDENEHNNEDGDDNVRKDEKNELKEEGNHEEKDGEEILGEEEVEEEEEDEEEDEDYGFESDNASCSSDVSENDYTSRKRLEETYKMLQIFSSKILRKKVHDAFQAKRAEDISRSLLAEEERESKLKKEKEEKEKKKKEKVKEKKRLQRLAKEEERKRLEIEKEESERMIREEQLRKTEEGRKRKEAEKKKREEELKAKQEEKKRRKELELERSRKEKEEKERKKQEAKKQRDEELLKKREEKERKERERQEKKEQEKQLLLKKQMEEEERLEKRLSELKLIQQKNKEADLEKEKQKQAEKSLQESRPLHMGTPTPLPNNSESLGSNGLHSHLFNSDFIPMNQSTLAAPPHHFPGFDSTPLLESHSLLNMKAGASSMYGANTFTNPFGNDSSGSNAHSLLNNNGLTTNVANPLLSEELGFQEQNKGLFNSALFDSKSLSGEGTPKVNEGSIWNSSVTPLNNLGTRETSQPAATAATGRHNSIWSSGEMSGTRTPSFSWSTPVANGADPLATNPHTSGVSVSATESLGIPQIELIQLESFKAAGRLPHISSDILSVPLLYHYTRSLLAGKLPSLKLEQFVAALSFDLGSKLQFSFSIFKDDKNEDVVKIVKRSAMLPANGMGGMNLNNLGMDMNYMNSSQLGGLNGGLNVFNGMNNINPLNCGGLENLAMNGNGLNLMNSDAGLLNALNGMGSMHTMNMNPLNMNLAGLNMNTNMNTNMDVGGHMGLGGMHMNMNVNMSGLNPLQDYSNSVGLDSKDPDRLQPAASAAASAVATADGRHSSRSGSVLASSTAATDTWPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW0</accession>
    <name>A0A1E3NKW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3 11">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_16589</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3 11"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017874.1">
        <property type="nucleotide sequence ID" value="XM_019161270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKW0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKW0"/>
    <dbReference type="GeneID" id="30177957"/>
    <dbReference type="OrthoDB" id="411145at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052829">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042538">
        <property type="term" value="P:hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043647">
        <property type="term" value="P:inositol phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016078">
        <property type="term" value="P:tRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000003">
        <property type="entry name" value="PAP-specific phosphatase HAL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006239">
        <property type="entry name" value="DPNP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01330">
        <property type="entry name" value="bisphos_HAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="357" mass="38596" checksum="E8737BE354185F71" modified="2017-01-18" version="1">MSISLATQYVKEAYIAQLAVKRAALLTRKVSEEHLNEQSIDKQDHSPVTVGDFGSQAIIINAILKNFPGDEVVGEEDSALIKEKKIEDLIYSHVETIQQADAAHDSKLGRLESAEGLCNSIDKGNSKGGKKGRIWALDPIDGTKGFLRGDQYAVCLALIVDGEVKVGVIGCPNLPHSLRDPGSKRGGLFTAIKGYGSYFQDLNEDISYPLNTSSNITVHNEYGLDQARVLEGVEKGHSSHGLQGLIKAKLGISQPSVNLDSQAKYCALSKGDAEIYLRLPTNPNYREKIWDHAAGYLLVNESGGMVTDIYGNDLDFGYGRTLNSQGVIAASKTVHSSIISAIKSIIGENGESLKEYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL34</accession>
    <name>A0A1E3NL34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017952.1">
        <property type="nucleotide sequence ID" value="XM_019161294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL34"/>
    <dbReference type="STRING" id="763406.A0A1E3NL34"/>
    <dbReference type="GeneID" id="30177981"/>
    <dbReference type="OrthoDB" id="28748at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="517" mass="59134" checksum="F5461EC7EE4302D3" modified="2017-01-18" version="1">MTMNNKEQLEDNAVPEQAPLKQKEGKVSRWITLLSVAVLLLSLRAWNPKAFFTLPANYYIRAWYLPTEILDHYIENILSDVSFVVPVYFESTLLFPDLGEAVDIQVDSRMHNEFFSLLNYTLQLIPGTNSEYQNGAYPKDDVFVNCLLSKDNAIHVDGAESYAELYFNLEGVDANDVPFFMTQLLVDHCFRDEVQKYAPDNFYKLDSTTTGQKQDSGLQDEGKFHLVHADFMSSNILAALLRSGSHKIKVRAEIHVVGTTVFSIDMEKAIEEYMQPLFEMLSDFFDLDIVVHNLGMEDSTPHERLNQTGDLLFPSELVNLPVIWDLYQRTKLESAQTDTICVNMLFYPFFQGGELIKEISIDASPIYSDHENTYLTVEEWGSIYFSHILYDSSKSVSKQHLKDCVWSFNENLLDALGVPNGNMAPAVRTKIFKRYMVMQYFTYYSSLLNELRNIISWNTISEAANFSPAKTRQFAEAFAKSLQERKRALYLARSGDLSASIAACRMMVDILQETLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLB0</accession>
    <name>A0A1E3NLB0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_52883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018027.1">
        <property type="nucleotide sequence ID" value="XM_019163168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLB0"/>
    <dbReference type="GeneID" id="30179855"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133410" evidence="1">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="64" mass="6822" checksum="5CA2C83E2455E3D0" modified="2017-01-18" version="1" fragment="single">EETTTATAALEALALAPALALAAAAESGCLRSRLVPRSPALQYHSVFTIHKAHFAQHKHFATVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLJ0</accession>
    <name>A0A1E3NLJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017551.1">
        <property type="nucleotide sequence ID" value="XM_019161169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLJ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NLJ0"/>
    <dbReference type="GeneID" id="30177856"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="242" mass="26973" checksum="1D80DE4CAB7B6AC1" modified="2017-01-18" version="1">MSEQVFEKKYVYTVLPPRTGLLRNSWYKRSNEWRPYPSSCSVGLAFIAVSLFVLGLVLCHARKVESPAILTGMFFFGNGLIQIIMGIWAIVDDNLFGSTLLLCYGGFIMSYGAAASDLFGVISSYPTTANYTSAIVLFLSGWVVFDFLLFLSTFKSTIPLFALTFCKWFFMLLLTIAESGNHSAVRVVSGIFCFITSICAFYAFYDGMQTTASSYLPINEPSWLRMPAGHKEPDDEDKIAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLW7</accession>
    <name>A0A1E3NLW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_10162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018248.1">
        <property type="nucleotide sequence ID" value="XM_019159641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLW7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLW7"/>
    <dbReference type="GeneID" id="30176328"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="39"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="926" mass="104207" checksum="076E795DA76B137C" modified="2017-01-18" version="1">MDRRTLLTHLSNTLSPDNRVRKASEQLLLGLHAEVVPQILSVLLSDDGSIPGQTKLVCLTLIKNRVAADWSHPATEEKTKTAVKNQIVEYIVQFQNPFSGANHPLIRMCVKIVDIILLYSDTTFQFDLLNLANDLVKKDHGSVDNFYIGVLFVRQVARRNRHLNTYELLDNISSNFCPVYLDFLQTYSKDLQGGVADANKKVLTHEILKTLNYVCNTRIPAYFQDVPSNALQALCSSLCELFCLLLSKNIDPSNVKWIVRFLIKIQAKAASATASAYPTDFLNLLQGSLVQSNASTIVDEISKSYELLSNLTNFADDGTKERTLYYFLIYLTRCIFALTYHSIEPHINMIISNIIVPTLSMNSEETELFEDDPTEFINTNLSSNFSYLGSSNTPFIDISNSDIKSASSALIVRLVGSKPELASPLISLAGQILSSGDKASLPCALNILKLVQSHIDEDTMNTAVSRLIGISKNLSNEDMWLCCLIYDFFSDLTTKTVDPIQSHLPVSLELSQPLPLLISSLKLMILKSDPRNMNLVQIMQLLLTVSENQTLEIINDMIDFLVEKYPQLLLPYSSELISSMCTSFLRVSEDEAYDTNNDNSNDATKETKLLGLLDNILTVVISIPDKQVILDLNNQLGCVISLVLDNAQLDLVESVMELVQEIDTKCKQVLNLDAVINSFKNYGYDYFDYYLGFFQSVYCYGDLKERSTVTELLGWIVSEHPSGFDPDDTEFVEFLTCLVSEMVVSCNANEDDGELSDKVFRTVLEMTFNSYEEKDEFWGNRYIFRCIIGGFLKKPMIVMNMFGDILDQVLVKFKNLIENGTWCTVYDLKLGMLGLLEIVKGCADNERIRNMAIEVISLLCDRYNGAVEHREKLLKITAGEENGSGEEQDEEYDLLSDQGFDELNKVSVLDDIDVVREIKTYLNVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLY4</accession>
    <name>A0A1E3NLY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_32511</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018275.1">
        <property type="nucleotide sequence ID" value="XM_019162772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLY4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLY4"/>
    <dbReference type="GeneID" id="30179459"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="640"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ47162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1008" mass="113768" checksum="9DF85839D97E46B7" modified="2017-01-18" version="1">MLRSFSKQLSRRAVVNSGAKARLACFKPVTSLRFFGQDTFLQGDNASYVDEMYEAWLQDPKSVHISWDAYFKNLNGKRPASMAYQAPPTIMPSPVGSLSMVPDSDTHVAGEDVMTHLKTQLLVRAYEVRGHLKAKLDPLHVSFGDAKKPAPKELTLEYYGLTDADLEKEISLGPGILPKFKESGINSLKLKEVIQYCENIYCSTYGIEYIHIPSREKCDWLRERIEIPTPYKFSADQKRQILDRLMWACEFENFLSTKYPNDKRFGLEGAESVVPGMKALIDTAVEFGVEDIVIGMPHRGRLNMLTNVVRKPAEAIFNEFAGVSIHEGQGEGSGDVKYHLGMNYVRPTTSGKTVNLSLVANPSHLEAEDGVVMGRVRAIQHSKNDVGEFNKALGVLFHGDSAVAAQGVVYESLSLTHVPAFATGGTIHVIVNNQIGFTTDPAHARSTTYPSDFAKTINAPVFHVNADDIESVVYLFNLAAEWRNTFKTDVVLDVVGYRKYGHNETDQPSFTQPIMYEEIAKKKQVIDMYVEKLQNEGTFTADDIEEHKKWVWDHFEESFNKAKDYKPESREWLTAPWEDFKSPKELATEVLPANPTGVSEEVFNKVGTAVGSYPSDFNVHRNLKRILKNRLKSIESGEGIDWSTGEALAFGSLLLEGYHVRVTGQDVERGTFSQRHAVLHDQKDDSHWVPLKNLSDSQADFVIANSPLSEYGVMSYEYGYSLTSPDAFVQWEAQFGDFANTAQVIVDQFISSAESKWKQRSGIVLSLPHGYDGQGPEHSSARIERYLQMCNEDSRGFPSPEKLERQFQDCNMQVAYPSTPSSIFHLLRRQMHRQYRKPLILFFSKSLLRHPLARSPKEEFLGDSVFRPVLDDIELGKSINDKEGIKKLVLCSGQVYTTLHKRRADLNNKETAFVKIEEFHPFPYAELKEVLESYPNIEDFVWCQEEPMNFGPYSFVLPRILETQREVGLQVPLRYAGRNPSASIAAGNKHLHHEEEEAFLDEVFGASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM27</accession>
    <name>A0A1E3NM27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tricalbin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15182</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018307.1">
        <property type="nucleotide sequence ID" value="XM_019160703.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NM27"/>
    <dbReference type="GeneID" id="30177390"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF1">
        <property type="entry name" value="TRICALBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="10">
        <location>
            <begin position="232"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="430"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="570"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="712"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="1048"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1262"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="866"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1281"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1330"/>
            <end position="1339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1520" mass="167047" checksum="23A79CB30E6715F1" modified="2017-01-18" version="1">MAATAQAQGPPQTAPAGDIPQINVTRPNRALQDASGVAPNGAGPTLDPRTAKQQQQQRVPPSPRQPVANGSANGTGKASKIAKPTDDVLGVPPTEDGGKLKRTTSSYSVNNEFIKPKEFTYKFPWKKIGSYVDSEKELTKEELEDLLLDSSANKLTTYIYEKYYADFYWNCSMIVGACFASWCFTYLGFGLFSLVFVLICTFAVYRAELRRFNINIRDDMQRIQSTENLEKKLESMEWLNNFLVKFWVIYMPALADLVISNTNQVLSQVDPPPPIIKLSLDEFTLGTKAPKIDAIRSFTKLGKDLYQMDWSLNFTPNDIDDMTQEELKNKIDPKIALGITIGKGIVHASLPILVENMSFLGNLRIRIKLGDTFPHIDIVSICFLEPPKIDYSLKPVGGNTLGIDVMSVIPGLSSFVNGLINSNLAPLMYYPNTIDIKPTELLQPPSAIGCLMIKVRAAEFSSSKTINPYIKYGPDGDLSKQHQTDIKSNTIVPIFNESTHILIDNLYAKLKFELFDLNKNGNSKLLGEAGFELQDLLQDQVLELNETKFTKKNKNVGKIVYDLKWYPVLQGEILPDGTKSNPPDSDTGIIELTIMSASDMDTSKSLVGKLSTYVEVYIDSKLIQTSRVVKGTNFPEYNFQVEDLIYSKTASILKLVVKDISSYTESIIAEYESKILDLTLIDNSGADAGKNRVSRDFTKGKGSLKFASVWKPLGSISGETDDDTTFVPPIGTFRVDVKSCTKLANVETLGTIDPYIEILSSGKTKGRTSTIKNSLNPAYDEEFFVPILSKNQKLRFNCMDEENKGKADRLIGDLILDISGFFNDPSKQNKTVSLSSKLTRNGKDVGTVNYALTYYPLLPVFSHQEIVQIKQKKAEAAEKAEDLDELEEQAKYLEDYKKHPDDYEWVDIDEEANELIQFETNKDKVILSLPEILQNNSGVLGINLISGTLKTKIAYVQATIDDHSFPDFISRKSKNGKLGSTSGECFIRDLRHSILNFRIVKNEIPVYKTDVLYETVDPFNVIDLLTKGFEEPIKIDLDGNKLEFLFEFVPSVDRSFETVEDTGLLEVNVISGSNLLAADRSGKSDPYVEGHLQDKQVFKTKIVKKTHNPNFGENFTIPVKSRRRQLLTFKVFDWDMAGENDPLGNVSVDLKNLPIEQEIVQDFKLDTQGSIKIGLHFKPGYIKSSNGTLLTYGAGLSPFNTGLNLATGAVGKAGGLATGAAGRAGGLATGAVGVVGGTAGTVGGIASGFTGGFSKLMKPFEETSGTNKEENLGSAGANSKQTHHSSHLKLKPSFLGGRSKRSESDAESLANDSFSRPSDVSAGNRARRVPSSTMLNDNGNANTNFNSPPPPNPNLIAFAAGGSPQQMRVPSSPGVINHDNYAKNRNSMDAVSVTTNAFSGTTAIAGRLSVLELHNTVDVKEALSIKVVMKSGNGTEKSIYKTKGTKFQEGMYKWHESAAFRCDSASTMIFSIRSHHTFGKSEEFGHGEILLEQVIGVKKNIAIAINGKISGSLVVNFNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMA7</accession>
    <name>A0A1E3NMA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71359</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018383.1">
        <property type="nucleotide sequence ID" value="XM_019163902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMA7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMA7"/>
    <dbReference type="GeneID" id="30180589"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="6">
        <location>
            <begin position="196"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="433"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="578"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="641" mass="75125" checksum="095CFF68A265A9B8" modified="2017-01-18" version="1">MSSAIYILDRDLNLIIQRHYRQDLDVSYILESFRAVYNRSHPIDLPIIESNGITYLYLKKKEVIFMSPVLGDIDVMSHMAFLEKFSQLLQKYFRHYNLVDQDTTEIKADLIRDNFVLIYELFDECLDFGIPQLTEFSILKDYIKLMIKPEDYHDGSHFNEQIDEVEKTVETEINSSISRTAMTKISWRPKGIFYNKNEFFVNFNEYLKFKYNHKVHKVILNQISGEIKCKSYLSGMPYLKMGLNETLNRQKTIFNNIQYHQCVDLNLLNDNTVEFIPPDGEFSLLTYQILDTQVLQPLILVKPQYRIFKKKDQYKLRIKVDIVTTFKRKFNMVDLKMRIPLVVKHPILYTDFNKSMKFKTKLGEVVHSLDDETIIWKIEKVQGSMRGEMLAEFDLITEKDLWESHLENQERVRQEHNDLYYFELGTEFTKLGHNKPTSRKIEHSQQHYRHPGDGGGSTDADINDDNSENDDGYKLGGKPTQTKAGIERNKKRRNKAGVMDNDVERVVTVEFQLQNMLYSGLKVDFLSIKEDQLKLQTFPWIMYCVFCCGDDYSFILSDEQFRNEIDEEDEKIMIERGRWLGGDSNTVPQSEPEPAEDESSKLTEDFSTALDHVGEISVPDGANLDFEEYVVEDEDEVQPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMD8</accession>
    <name>A0A1E3NMD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72603</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017656.1">
        <property type="nucleotide sequence ID" value="XM_019164246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMD8"/>
    <dbReference type="STRING" id="763406.A0A1E3NMD8"/>
    <dbReference type="GeneID" id="30180933"/>
    <dbReference type="OrthoDB" id="340550at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.60">
        <property type="entry name" value="F-actin capping protein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042489">
        <property type="entry name" value="CapZ_alpha_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017865">
        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00748">
        <property type="entry name" value="F_ACTIN_CAPPING_A_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="279" mass="30980" checksum="B3E54DE166D9E4CC" modified="2017-01-18" version="1">MVSEVESIVRQIVKTSPPGDDRTIIGDVKALLNNADADKLIAGVLRENYTTELAQDGKKGGDAKLVRLSSGQHTIVSKYNLSGIKFFDIQQGIVFDYDFINGRVIDVEEKLPSGVDGKAVEKLQEQLDDYIAAHYNELSRGMVIPGANDSLTVVIVGEKLNDSNFYNGKWAAVYEWDEQSGRLSAVVKVRVHYYEDGNVVMNTAKEEDLGQISGVGAVIDKIAEYEKNYELSVLKKVNVLNEQKFKNLRRLMPISRSKIQWGRAIGNYKLGQDVAGGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NME1</accession>
    <name>A0A1E3NME1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018415.1">
        <property type="nucleotide sequence ID" value="XM_019160744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NME1"/>
    <dbReference type="GeneID" id="30177431"/>
    <dbReference type="OrthoDB" id="3984059at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="214" mass="24083" checksum="F9051F2ECF5E0EE5" modified="2017-01-18" version="1">MAVPLPQYDSVLMREHLFRDTNQLEAVLSKDTDTFRPVKEVGLGLFNYRLVNIDLVSESEGLQSTSFRDIMARALKFTQATIIDTILFVLIVSLILLLFSWLYPKVNFKKRGFSKQESVDLENQYSSSSISAESTQFEKAQYCTQSPASSLSNTQDSLSCFMSSAYNAPVTPTSSSVEKFRTPKTPVGMGGMNNYTRLPETVDSKLLMNRLRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NME8</accession>
    <name>A0A1E3NME8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15267</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018410.1">
        <property type="nucleotide sequence ID" value="XM_019160742.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NME8"/>
    <dbReference type="GeneID" id="30177429"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF129">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 16F, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="2168"/>
            <end position="2356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="956"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1230"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1291"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1990"/>
            <end position="2043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="35"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1291"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1990"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2029"/>
            <end position="2043"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2357" mass="265338" checksum="080E3D1F7B331A1F" modified="2017-01-18" version="1">MKKFLGLKEKDKDREKDRDRDRESSSRRKQYLNYHSPHNASPAHSHAQSHAQPQPSSTSPSQPHPHAQSQAQKKPATQYRPGHPLHQQQQQQQQQPALPTQLHHNAAGSSSRSSIDNPLISRSSTLSNLNNSVHSLGTHPQTYAHGSQGPSRTPSFHGSVKSSQSSSNLASTSASMHHQFQAPSKGQPAAPAAVPSAASDHPISFLSSPSIGKGSPLAADTFLQPSSIPDPINLDDALNDTFNENDLAADTTMEAIEKTTNLSAFPSSSSSSANPIPSSPELEKTTSSTDNSGFDNSKLHSQPQLQPQPQRRESVHSVATTTATLVPDSSRKLQGEIKRTGRGVLPKVLSSNRSNYAPSVYSVDQSFISSARSTVFNSGISTNTAFSSSNQSNVKNSSSGQYNISSLVKPEWDNSSKKSSWANRILDPNDSRLFRLELKGSYLLVHKPPAELSFAKSMNVKVSQQSSGSGMGMILDNEFVKSKTDVISSSQPIHKTESNVETEDAPLDGQVKAEAENEVPVTMNPLVQSDSMLLSMAIDEYESAPGLGDAKKLYDSSRNTNTELNDLVLDADREAFDNYNKLLGARSVEMSQRPSMKPSSQGSKHLPLIIHDLYYQSPSCPHPKLVFDMTSGLILRGTLEAMCHTILFYPSDRMAEKLVEILPLVSSLSEPLVYFLKYLEHFTDAEVLKKKSIIMTDMETGILLNRVKRIIEITLEKFNGTLLDDEIFNLLGNLVNLLSRKSGKYLRESDVKQISEKLIAHRASLLRLISFEKVSTSGNILAPIEILQVSQTDLISGEKFIMLSTESLAHEIHAIDAHFMKYWSPRSDRSTIFSRLETIEASFEYWRFNPLQFIPPIHTHYLGRLLAFHLFEDKSTSVSPEKRSHVLMKWIHLGSTLCEQGDMVSWLGIAIMVCSQPVLRLSETWGHVERSYLQTIKKKWAPVVFEVRKNELFGSKDPLDKQNHSQTHGSELPVSDSLKNETNSNIRIMITNRVGELYAKEDAIPHFGELFTADTADFRNEQLPSFETHASNGSSEPFDLFKTDFIKNYCSYLEVVGWNLKQWDNYYDTITNKPGVKSKFLGKSESSNDPSIIDGCKDKSLSNILKTAITYNASEGPFSLNYFMGLSVQAEPPYVGQFARFHGTSRSPLFLGSYASILFPEVLPYYGIYDRRELIGAIGGANIIELNETGKHKNRNLSLKHVRDLFNAGSEEFRLEDDSIIFKTTELVNEASSKTTEEEPNKRSRPSSVLFENPSHHKHMSTLSHESFSLEDYITSYQTYLKDAINESTSSRAAAEDSTLTSSMNKLNTDSKEGESKNVSNSPKSASSVDIVATAASTERLVDLLVLTASVFGTHIRLEDIKKYSRKTENEGPILLQMDDSAFTCTFFATYRVFYTTRQLIDSLHKRFDGSKSAALSIAKFMNNHSSMTDRGDLFPQWHDEITDDNETWSKINWKFVVQIQLGVIEATLILANDYFRHFMDDIPTKAAFDTFLEMMDVAIVSEWPKILKWLKVNSTPEIVSGVLDVYKSLQSAYKQVRNICIRKSYTPQCGLVELSFTDELTNIPEYMKLPPSTDIDEISIFINKLDDTIACVMNPIRSEDWIDTFEIIQMLITRSPLAMFNYNYQDPSTHPDLILVSNIYNWIMTLSDNENISKDPSDKLINNLPPSIRSVFKLYERIQNYLLMQIVDLECNLDERIERMSTLLKIIQICHARMKNVKLFDEVDNTSKIETSPYVPSFIESVVVNTIMLPESRFFSHSWRLAAEVWKADTDEEWSCDKLADMLPKLSQYESATIQPEALSICPGWILSRLVELACFVPNMDIDNTALINFNKNRFVYNCVLKIHGLQSKSKKTGSPYMSEFSFLFEFQGLIPSLKAMYQRSSHERSVEKLEKCAVFSSHVKEQIQLIKLEQNKRELLVRQLEKQNLTTVNKHISQQVEPLAAENDDASIATSTITNSQSFVANSSMLDNVSTYTDGDAASIVDTTSSTALNTVDPNQSIASNQSGHSTPSSATLTRASNSRGQKLTLQQQQQQQQHSNSSSSKFKFGFFKSRPFSLNINNFNQPVTQKKNVELNELPSASILGSKQKPYLTIMLKDVSIFPTYRTPNSFTIDIGSSSSNHEYTFQTNDEQEAGDWIYQLLYAKKHWFYSRILNKQYAHSNVKLTFGAPLVFVCERDSSPIPLIVEKILCEIEFRGLEEVGIYRKSASLSVVQQIKDEVNKMGDFNMENSLVFDIHNLTGCIKAYLRELPEPLIGDDLVKEISKVREVAQDEARFEIYKTVLPKLPIYNYNLIERLSRHMRMIEEYKSQNRMTSYNLATIMGGSLVEGCRPETMREAFGLMNFICEDWILHYERVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMT3</accession>
    <name>A0A1E3NMT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_11680</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017816.1">
        <property type="nucleotide sequence ID" value="XM_019159825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMT3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMT3"/>
    <dbReference type="GeneID" id="30176512"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="354" mass="40656" checksum="BAEEBDE46968E1AA" modified="2017-01-18" version="1">MPPKKNGGKNQQKELEKKAKQKSKAKNADDKTFGLKNKNKSKKVQEFITQVKAGVPPDLKKQQELAKRRAAEKKAADEAKKEALKLLQGSVGTQKVPFGVDPKSILCEFFKLGTCTRGKNCKFSHDMNIARKSAKRDLYTDDKDDKEDKLADTMDNWDEEKLRSVISSKHGNPKTTTDKVCKFFIQAVEDGKYGWFWVCPNSDPKNKVECKYRHSLPPGFVLKTKEQRRLEKLAIENQPKITLEEFIETERDKLPKGNLTPINPETFAKWKKEHRQKRLNMQTKDKPQLTGREIVLKRFEDKFLREEEIGADYGTEIDMSQFKKAVEDIQDENEPKVKDYGDGTGAFADANRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ3</accession>
    <name>A0A1E3NMZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase catalytic subunit A</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_50228</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Vacuole membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018589.1">
        <property type="nucleotide sequence ID" value="XM_019163145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMZ3"/>
    <dbReference type="GeneID" id="30179832"/>
    <dbReference type="OrthoDB" id="1676488at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001296">
        <property type="entry name" value="Vacuolar membrane ATPase subunit a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007868">
        <property type="entry name" value="Hom_end_hint"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007869">
        <property type="entry name" value="Homing_endonuc_PI-Sce"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05204">
        <property type="entry name" value="Hom_end"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05203">
        <property type="entry name" value="Hom_end_hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="15">
        <location>
            <begin position="486"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1112" mass="122345" checksum="9DCBE4DE45EBC9AC" modified="2017-01-18" version="1">MAGAMENARKAIKNLSIDDEDESLYGQIYSVSGPVVIAENMIGCAMYELVRVGHDNLVGEVIRIDQDKATIQVYEETAGVTVGDPVLRTGKPLSVELGPGLMETIYDGIQRPLKAIKEMSQSIYIPRGVDTPALSRTVKYDFTPGQFQVGDHITGGDIFGMAFENSLLSDHKILLPPRARGTVVSVAEAGSYTVDEPLLEVEFDGVVSKYSMMHTWPVRVPRPVASKLSANYPLLTGQRVLDALFPCVQGGTTCIPGAFGCGKTVISQSLSKYSNSDVIIYVGCFTKGTQVMMADGQDKVVEDIKVGETVMGKDGKPRSVIGLPRGHETMYKIHHITGHRRDAEVHGLMDFTVSADHKLVLKTRQNALITTRRYGGKEYAAVVFFSLEKSKYDIEMVRTKTKVFGYHVHGSKARAEEKAVKFAAELNASPYIDWVIEAKDYHLVSDNVKTNTTQMINPVLYESGSLGKTLESRGQEKSSAAYLAYMLGLWVGDGHMDRAAFSFDPSDTELFARVSEYSEKLGLSASSHEHLHRYFANSNVSRPITPKIDNNDFLVDEDMDTDLTDVTYESENNESSFGSYGSRGDMTVTIHDAGNDIRNKSNVSNVFWDMVESFGVRYNTSSGYEKAVPSHIACDDIKVREMFLSGLIDSDGCVKLNSDGTKNATITTIHKTVSEGIVKIARSLGIKVSVGTEDEKIDSNGVRHQFSYRVFLNGKALAGVLSNCALARNSADAVSFTREPVLFHFDLIESEMEDYYGITLDESSDHMFLLSNMALVHNCGERGNEMAEVLMEFPELYTEISGTKEPIMKRTTLVANTSNMPVAAREASIYTGITLAEYFRDQGKNVSMIADSSSRWAEALREISGRLGEMPADQGFPAYLGAKLASFYERAGKSIALGSPDRTGSVSIVAAVSPAGGDFSDPVTTATLGITQVFWGLDKKLAQRKHFPSINTAISYSKYTNVLGKFYDSNYPDFASLRDKIREILSNAEELEQVVQLVGKSALSDSDKIVLDVANLIKEDFLQQNGYSTYDAFCPIWKTYDMMRAFVSYYDEAQKAVASGAQWSKLADATSDVKHAVSSAKFFEPSRGEQEVGTEFDKLLSTITDRFQEASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ4</accession>
    <name>A0A1E3NMZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_33149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018111.1">
        <property type="nucleotide sequence ID" value="XM_019162801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMZ4"/>
    <dbReference type="GeneID" id="30179488"/>
    <dbReference type="OrthoDB" id="531564at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044980">
        <property type="entry name" value="NDUFB2_plant/fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36987:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36987">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="7002" checksum="379DC953E1726CAF" modified="2017-01-18" version="1">MSHAAAGYTRHGGAPVPKAGPFYRYSATFLGATMWFWLFYRAKHDWKQWVGIEHPWDHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN60</accession>
    <name>A0A1E3NN60_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_138231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017986.1">
        <property type="nucleotide sequence ID" value="XM_019160115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN60"/>
    <dbReference type="STRING" id="763406.A0A1E3NN60"/>
    <dbReference type="GeneID" id="30176802"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="476" mass="54116" checksum="5D3B2FFBA69C7CDF" modified="2017-01-18" version="1">MCNRRGRREERRLRKGLREQGRWFNPPPLSGSFFRRLLPQTSDNARDVVGWRNRRAVETRRQVRAVRTVLNRHTMTRMVVARTWGWCAGAPRGVGGSRTRRAWRTCYSTQTDPYTDRVEKARFDCTAALGRADPSSVLLSKMYPAYLRDSFVAFRALNMELSKVSFGFQPGATAKSREFSNLKFAFWLQQVEACETLTLENAGAVLPRVNEPCTVLLADTVLRHLRLDAGMLKQMVHSHQHFYNHNSAAGFRSLDDVCSFGEGTFSQMNYLMQSVLLSPQLSGFADPGVALLELPGSDDLRHGLTDISAHLGQATAVGSFLVGLRYFAEKKHTLMLPTDTLTKHRLSEEACLRYLQGRAADADPDTKERLKNVVFDMATRANDHLISARTKLDLVKTHVQQLAAAPDTPAPVCSLLRHQKRGLPDCLFLPYLSGLPTTLYLERLEKYDFDLLHPNLMYKEWRLAFRLWNAERTRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN75</accession>
    <name>A0A1E3NN75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-aminocyclopropane-1-carboxylate + H2O = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16933"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58360"/>
            <dbReference type="EC" id="3.5.99.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ACC deaminase/D-cysteine desulfhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018006.1">
        <property type="nucleotide sequence ID" value="XM_019161313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN75"/>
    <dbReference type="STRING" id="763406.A0A1E3NN75"/>
    <dbReference type="GeneID" id="30178000"/>
    <dbReference type="OrthoDB" id="10266364at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008660">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019148">
        <property type="term" value="F:D-cysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009310">
        <property type="term" value="P:amine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06449">
        <property type="entry name" value="ACCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005965">
        <property type="entry name" value="ACP_carboxylate_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01274">
        <property type="entry name" value="ACC_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780:SF2">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="8">
        <location>
            <begin position="12"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006278-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="338" mass="36915" checksum="8F9CEC4387C9C8A9" modified="2017-01-18" version="1">MALLDKFEKYDLLFGPSPISKFENLAKELGGKVNIYAKREDRNSGLAFAGNKLRKFEYIVPEIIAGDYTHIVSIGGIQSNQTLMTIATACKLGLKAVTVQENWVPIPEAEKATYDKVGNIEAARILGGDVRLVPDGFDIGMRKSFEEALQELTSQGFKPYPVPAGCSEHKYGGLGFVGFADELIKQEKEMGIKFDKIVVCCVTGSTAAGIFVGMAQYGRENDVIAIDASGKPEKTFEQTLRIINHTSEILGLGKKFDTFNFDTRFGGPVYGIPNEGTIEAIKLLGKTEGTLTDPVYEGKSMQGLIELVKEDKFEKDSNVLFVHLGGASSLSAYQSYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNJ8</accession>
    <name>A0A1E3NNJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol phosphate-mannose biosynthesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15596</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of the dolichol-phosphate mannose (DPM) synthase complex; essential for the ER localization.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018782.1">
        <property type="nucleotide sequence ID" value="XM_019160886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNJ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNJ8"/>
    <dbReference type="GeneID" id="30177573"/>
    <dbReference type="OrthoDB" id="311279at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180047">
        <property type="term" value="P:dolichol phosphate mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009914">
        <property type="entry name" value="DPM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15039">
        <property type="entry name" value="DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15039:SF11">
        <property type="entry name" value="DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07297">
        <property type="entry name" value="DPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="82" mass="9238" checksum="CDA7D6680C1A9F9A" modified="2017-01-18" version="1">MQDQQVGLVMLLVATLIFIYYTIWTFVTPFLDDDSIIQNFFLPRYYAIALPVVALIVGISIVATFVGLVIVKSVQKKKGKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNS8</accession>
    <name>A0A1E3NNS8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA repair and recombination protein RDH54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_27671</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018840.1">
        <property type="nucleotide sequence ID" value="XM_019162507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNS8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNS8"/>
    <dbReference type="GeneID" id="30179194"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032392">
        <property type="term" value="P:DNA geometric change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030491">
        <property type="term" value="P:heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18004">
        <property type="entry name" value="DEXHc_RAD54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000020">
        <property type="entry name" value="DNA repair and recombination protein RAD54B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="177"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="512"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="814" mass="92801" checksum="EBF86AB6DCD8AC12" modified="2017-01-18" version="1" fragment="single">ISKYNVLYRKFTTKKNKTWEGDAVLSLNSSTNFATIRNEDDIVIGRIKDADRVIFKGCFKCSGFEFELDDEIIHSKVPKLNLSSTPRHIPQSVIRAHKPSTFKSPLIAEAVRQPSPDMIQFNDFTHDKSDPLFMPDLPKSIDASEKPRMVVVDPSISSKLRLHQREGVKFLYSCLMGLKSPDMRGAILADEMGLGKTLQTITLIWTLLRQSPIAGQKPTINKVLICCPVSLVNNWKSEFSKWLGLNRLNVLTINSNKYQNEKQDVELFGRNNVYQVMIMGYEKMQSMSDLLSGIRFDLLVCDEGHRLKNSENKTMKTLESFNIRRRILLSGTPIQNDLVEFYTMANFTNPGIFGDLKTFQKEFIKPILDSRDPNCRNEKFIKNGKKKSKELVDKTNNFILRRSNLELTKYLPKRSDYIILVPPTPLQLQLLKTVIETKKFKSLIELDESVSSSATGGAFNLINTFRKICNSPSLLKDDSFFLEVCERNSDSLDDVNFRKQLSKKVKSGKLLVLIKLLGMLHSDGEEKVVIVSNFTATLDIIESLFRSLNLVFLRLDGSTASNERSKLVDRFNKSSPDKVFALLLSAKAGGIGLNLVGASRIVLYDNDWNPAVDLQAIARIHRDGQKREVKIYRLLTNGCIDEKIFQRQLAKQDLSDRFVDQGGGEKELFDRSELKDIFTVQFDKVENHDYSNTHEMMMCECKGDGITSIDDSCPREKNEETEAEAEVEPDFTSQRKVEVMKFVSALTFADHYKVEEEDALDMKKQQLRRCMKGYKHLNPVSATNRDFRTDDAVLNKLLNEQDKAKPLVSFVFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP87</accession>
    <name>A0A1E3NP87_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein YTM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">YTM1</name>
        <name evidence="9" type="ORF">PICMEDRAFT_15790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR12/YTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019033.1">
        <property type="nucleotide sequence ID" value="XM_019160969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP87"/>
    <dbReference type="STRING" id="763406.A0A1E3NP87"/>
    <dbReference type="GeneID" id="30177656"/>
    <dbReference type="OrthoDB" id="10251381at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110136">
        <property type="term" value="P:protein-RNA complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03029">
        <property type="entry name" value="WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028599">
        <property type="entry name" value="WDR12/Ytm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="8">
        <location>
            <begin position="12"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="286"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="371"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="453" mass="50171" checksum="51C112055AE6D7E0" modified="2017-01-18" version="1">MVETQDNIKDQVKVKFFTRDEDESLHCSQNPIYIPVSLKRFGLSEIVNHLLETETPVPFEFLIDGTILKTDIQDYLVKNGLSTEAFLTLEYTRAVLPPKFLSSFNNDDWISSIDSISSMELPGSDKTSIVTAPKILTGSYDGIVRIYNSSGKVETQLTGHSQPVKAVKFISPTKFITAGMDRSIRLWRAGVVSDEDIGKDTQAGHTFALLDYHKSAVSSLDLSLKTNRIISGSYDNSLALWSTNPKEMNTSDPIDASERLSSASRKRLKLAIKDSSIRRKAPLSILESHRAPVEGVLFDENDPSVAYSVSQDHTIKTWDLVTARCVDTKQTNYSLLSLVALKSMNLLATGSSARHINLHDPRAAKVTNSQLIGHTNFVVSLATTDNEYMLISGSHDGTAKIWDIRANKAMYTITRQSEEAKSKVFAVNWEKEVGIISAGSDKKLQINSSAEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPE7</accession>
    <name>A0A1E3NPE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_32504</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:34718"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:133977"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019072.1">
        <property type="nucleotide sequence ID" value="XM_019162770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NPE7"/>
    <dbReference type="GeneID" id="30179457"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000026">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="48"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="129" mass="14528" checksum="454116C87BE2001B" modified="2017-01-18" version="1">MNTTLVIILLFIAFKLFSVLRRYLTTTPRQMSQQTISQVEELIKTKPVFVASKTYCPYCSATKKLINEIYPEAYILELDTMEEGSEIQDALAKITGQRTVPNVFINGKHIGGNSDLQSLGKEKIKKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPH4</accession>
    <name>A0A1E3NPH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_10925</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019110.1">
        <property type="nucleotide sequence ID" value="XM_019159730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPH4"/>
    <dbReference type="GeneID" id="30176417"/>
    <dbReference type="OrthoDB" id="1721126at2759"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3280.10">
        <property type="entry name" value="Siroheme synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028162">
        <property type="entry name" value="Met8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14823">
        <property type="entry name" value="Sirohm_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="8">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme biosynthesis protein Met8 C-terminal" evidence="7">
        <location>
            <begin position="205"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="277" mass="32088" checksum="C5876DCBB38E9AE7" modified="2017-01-18" version="1">MTDIVPPPPNGSLMLAWQVKNKHVLVVGAGDVALSRVDHLLQANAKITVVTGESVHPILRKYHEMGLLEDLVVRNFRLSDLKLYEPKDIRQRVMELDNDNDESDEEYEVLSEEAVKLIEDFEQCKFKMVLTCINNYPLSLRIWQKCKSLGIDVNLADKPANCDFYFGSVYRQGPLQIMISTNGKSPRLCNRIKTKMLIPMFEDLDLEHAVENLGYLRSQLQKVYHPGDDTKVIKERMEWNRKITDFYTIQDWCAMKHESIDKILKLYPEFPSVDTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ9</accession>
    <name>A0A1E3NPQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_27728</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019224.1">
        <property type="nucleotide sequence ID" value="XM_019162510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPQ9"/>
    <dbReference type="GeneID" id="30179197"/>
    <dbReference type="OrthoDB" id="762982at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="711" mass="82246" checksum="96D486AD510311BD" modified="2017-01-18" version="1" fragment="single">NSFPGTAHFRVDKFPANKTKSCVECRGKKRKCSREYPTCSYCSRHRRLCVYGVNDTSPTEKHARLLMNLKSSMDQYTNVQKNSENVFENYDGPTPELYSEQTSQQELQRVNVKRESEVLDELPSDGLPAAKTRKSECLSSLKKQTADLKSLRPQITEFDESFILDPQYEARPRAESYNKRLVHPEMRVGSVYTKLFNSSLNLNEQSVPPDLDLLESFIPNKAEADVLMDRYRSSVHPIVPILDLQTIFPLYERFWGNKESVSISFYIIIFTIFYASSVSLFEERCIRIDETVDREELIKKMKFFVGATEAALVMDDFPNKISLVGLQASTILYTIVRNDCKTDDFVSISTLVRYSQLLELNRDPQNYHHLAKSKDVQLRRLLWWQIYFLDCTTALSTKLSPIIQPCEYDTQLPNEFRKRFNGQYMLDQAIAFANGRFRWAECTNKLLKASFCVKPPSDQIIVRLTREIENLSFCCSSLIQRMLDPLNIMPSEEQFVKFASSMLSTLEDRCFILLDVLFSDQKRVMPVEEVSSAGESSTESKAIDTSSQVHLLSEYLIYGSMPRNAIFVWEVRKYQPIQTLLSLLKGIVLDAMDKSFSLTSCRMKKRIEIIDQVFIKLNYLSEHTTKLCKQRWKMLKELKDITWDSLFRGESCTSSTSSETSFIQNGNMPHNNMNLLIESDWDKIFKEMADIQSIIDENINLKVWDNASGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPR3</accession>
    <name>A0A1E3NPR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED18</name>
        <name evidence="9" type="ORF">PICMEDRAFT_40924</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018646.1">
        <property type="nucleotide sequence ID" value="XM_019163113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPR3"/>
    <dbReference type="GeneID" id="30179800"/>
    <dbReference type="OrthoDB" id="5348092at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="253" mass="28748" checksum="F9293AD94A7BDDEE" modified="2017-01-18" version="1" fragment="single">MVQQLSLTAQVASPRIERLLLTLETLTGNICSPMHQHSVILKPRYPFTPEPIPGKFIQIESYRIRMNRIWEKGFPKEFLNKDEKENEADVFGESITKPNPNVNSSIWTLQLTDIPAGGKNSVLIQNIYETTIYQTDDVIGYLDELGYMHEAEFWTDGVRFYYGNVIIELSRLYIKADEMVIDPVEDSETSKLSLLDPTGTCHVKAYVNVGALNDLESAAIGIKQLESLKKELSEIVELHIPDRTAMDSRINSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPR6</accession>
    <name>A0A1E3NPR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_50592</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018821.1">
        <property type="nucleotide sequence ID" value="XM_019163147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPR6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPR6"/>
    <dbReference type="GeneID" id="30179834"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="157"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="480"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="603"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="661"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="236"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="705" mass="78580" checksum="4020ECDD7834B3D1" modified="2017-01-18" version="1">MQTPTRNTSHYRNASGNNVAALRPSSTHNQRSVSERLTLQKDAHKPPVPNNNNAYPYTTSNSTLNPLPRAGSRTASRAASRVLSRGNSAFPTPSGLNSISSCTPQRRTSLSRSTSRSSSSIGTSSVSSSRIPSNTIYSDSPLSLSTLENNSQRKSSRIRVTVRPCPLTAEQNGRNMPTPWNIDCASSQIEHPDLGFFQYDHVFATQDDNQCVFDVVGSPVIQQCLDGYNGTIFAYGMTGSGKTYSMRGVVRNSVSLLFDNCTKKKINYMTCSILEIYNEKLNDLLAEDNKNTRFPNLSSDLRIVDDDKFGISVRGLREVRVTSTDQLLALIDQGELLRCTDSTDYNYRSSRSHFIIMLKLFMEDNEGTEIVSSLNFCDLAGSERATSHTDRRKEGGYINKSLLALGTVITKLSENVGSSANVHVPYRDSKLTRILQPSLSGGSMVSILCTIQLGLNVIGETTNTLRFGFRARNVVLNVKQNTADNDLGKLMQENENLKLEIAELRSLLQISNNNSAGGIASMNNKTSMCVENDDLYYELVAENNILNEQVEHLKRLQLEDNIRRSQHCNEELGKLNDLLDGLDLDPGTRKKSKEIFGIMEIELREYNSRFNETESYVAHLENRIRVSEIELARYRQLQQQEQLDSSSTVLNTVATLNISALKRADHAKDELLEELQEEIAELKQTMRRKDAMIRALQKVGVADGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPT6</accession>
    <name>A0A1E3NPT6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019254.1">
        <property type="nucleotide sequence ID" value="XM_019164107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPT6"/>
    <dbReference type="GeneID" id="30180794"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="108" mass="11409" checksum="C4C5ECE21310C64C" modified="2017-01-18" version="1" fragment="single">DLESSSADSVASTELEDSFAGCAPPSEPQVLSYKESLAHLTDNMNLAANEAQKLPDISTNTFDYQESDRAIALADPPSENPASVPSDEDHIDASSEPYFPSLSSGLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPT9</accession>
    <name>A0A1E3NPT9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_123386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019232.1">
        <property type="nucleotide sequence ID" value="XM_019159914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPT9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPT9"/>
    <dbReference type="GeneID" id="30176601"/>
    <dbReference type="OrthoDB" id="3992718at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="560" mass="63296" checksum="092EB2832112479E" modified="2017-01-18" version="1">MPFRAEFELKAKDSRALWTKAINAFHTISDSFKIVIFQGTESGVDALGRKCFSEIEFITMNKTKTTVLNISFKECFFKRFSITGEIATGESERYRVTGNQFTHNYAKSYTLIVNSQDMNILFKDCGDDAISWKVFMLAGNEISHMVYSHKLFVEFDTRSGMKKKYSIAFRPGINTSDDRIHCIYLKTLENQKMRDEILEGAANGSGAAVGEFDDANLFDEEEDERIHRVALNTLILRRFIQSFPPTLEDFQIGIAPIEGTIFFKGFNRQQITSKVESMSNRPMTLSICMRLDQIVYNNIRDLGLRGDEDNNEKSADEHHISFRLKNFKTFIQIISGNLSVFNDEDTNGKSRAKAVESFEEGITLGDDDNICDIMFSKPGYPIIFEKRYFMNGVSEMQECSSVTLTEVTDGESRKLVLEAGSTNVNDKLANFALQAANGFVDDRLRRVATTTGRVEDGLRNTDVGEPLFVADEGGDMEESNMGGVSTSVGDGDRRSVGGGSEDFERIFWDNSKFHQSHVVEKALGTEQELEREHREEEDEKEVGEEYLGPTQDLQVKGIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ03</accession>
    <name>A0A1E3NQ03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dihydrofolate reductase</fullName>
            <ecNumber evidence="2">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_58439</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019258.1">
        <property type="nucleotide sequence ID" value="XM_019163299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ03"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ03"/>
    <dbReference type="GeneID" id="30179986"/>
    <dbReference type="OrthoDB" id="10267436at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110136">
        <property type="term" value="P:protein-RNA complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="132"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="217"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="266"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="389"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="431"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="DHFR" evidence="10">
        <location>
            <begin position="506"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="710" mass="79776" checksum="8E8AE08C577B1772" modified="2017-01-18" version="1">MATLIPPPSKRQKKDAQQAREVDLIPTDLPNVLIKFQASDTGESVGGSIRVPGAITEKQLEELLNELSGTADDPIPYTFSLETDKDLVDIRDNLYTSVLKPGLKTTEDFMTLVYTPRAVFKVKPVTRASATITGHGATILAAQFAPNTSNKLVTGAGDSTARIWDCNTQTIQKTLNGHSNWVLCAQWSPRGDVIATGSMDSTIRLWDANNGNCLGTLIGHTKWISYLSWEPVHMVEPGNKPRLVSASKDGSIKIWDTGTMHCIMTMSGHSSSVSCVKWGGMGLIYSASHDKTIRVWDAKDGKCLQVLKAHAHWVNHLALSTDYSLRVGAFDYKSEEPKNEKEEQEKAKKIFEKVAKIGGKLVERLVTASDDFTMFLWEPSKSNKPLCRMTGHQKLVNHVSFSPDGRYVASASFDNSIKLWDGRDGKFIATFRGHVAPVYQTSWSSDCRLLVSCSKDTTLKVWDVRTRKLMSDLPGHQDEIYTVDWSVDGKRVASGGKDKSFITSPKVSFIVAALLPELGIGYNGQLPWSLKQEMKYFRKVTTATVDPKKKNAVVMGRKTYYSIPQKFRPLKNRLNIVLTRNITKLQEEMKEELAANGDILRLSNSLKHTLESLTEADQIEEIFIIGGAEVYNQLMAQNHDLIDTIYLTEITHNNKLDMDAFFKLDTDLWKKCDQQELTNRLRLKGLHEEFQLADNQQNEFTFHFTMWEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQE7</accession>
    <name>A0A1E3NQE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pyruvate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_69981</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019447.1">
        <property type="nucleotide sequence ID" value="XM_019163521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQE7"/>
    <dbReference type="GeneID" id="30180208"/>
    <dbReference type="OrthoDB" id="3970464at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090180">
        <property type="term" value="P:positive regulation of thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF30">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE ISOZYME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="12">
        <location>
            <begin position="6"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="10">
        <location>
            <begin position="203"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="11">
        <location>
            <begin position="410"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="64049" checksum="8B636B38F55F6A4B" modified="2017-01-18" version="1">MPEQITLGRYLFEKIKEAGVHSIFGVPGDFNLALLDHVKEVEGIRWVGNANELNAGYEADGYSRVNGFACLVTTFGVGELSAVNAVAGAYAEHIPLIHVVGMPSISAEKQKLLLHHTLGDSRFDDFTAMSQKISIKLKVITEFDDAPKVINDLIETAILTKRPVYLGFPSNFSEALIPASDLTKYKLKLSLPPNNEESENEFVENVIQLMEKAKNPVILVDACASRHAVIEQVEEVARLTKFPVFTTPMGKGSFNEDNSEYYGMYIGALSAPDVKEIVESTDCIISIGGLLSDFNTGSFSYSYATTNVVEFHSNYCKFKRANYEDLQMKGALDNLISKLSKVSLSYQPKLSPPKSKYEYQTAKTCPEGPLIQEYMWKRVSYFLNEGDVVVTETGTSSFGILTTTFPKHVKAISQVLWGSIGFSLPAAVGANFAVDDFKKLNSSYPERRVILFIGDGSLQLTVQAISDACRWNIKPYIFILNNKGYTIEKLIHGPHESYNNIQPWKHDAILNLFADKVEYENLKVSTNEELDELFKNEKFNVPDKIRVIELMLDEFDAPSKLRLQAELSDKINSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQE8</accession>
    <name>A0A1E3NQE8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_10723</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018876.1">
        <property type="nucleotide sequence ID" value="XM_019159710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQE8"/>
    <dbReference type="GeneID" id="30176397"/>
    <dbReference type="OrthoDB" id="3997269at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="469" mass="53765" checksum="709CEE77E5D789A7" modified="2017-01-18" version="1">MEGGVPPTRSALSPSFCFFTKLRVLVIRNPLLKLSAESTQYCRMISSKAFSSVKPLGQFRTIVTFNFALLSKHKDVNVVGKYDKNNSTEDGQPNSVLDMLSGDINLTDHNIEKVVRKQVTELERINRYHDRNVVRLANKLSYIQENIGLRTKFSDEKRTRLFASELIDMPIEKVVEMVKTLTSAKSKSGLKVETDLTVLLSHLVFRNSLDANLFSRIVVKLGIPNLKQVQNKLASDPEDFIQGWNDDGRSRVRCGMALAARYKMLKDFKSAQSLIRSEFQTVWLKTLVENEKILNGSDIKNMINVVDGLIEYGYLVTCAVRTNSFRVIYSFWEIHPNDGLIKEWLEKGVGKVDCIDSDIGPNVYQKFIFELYSNPAISTVNKWKVKVLNVSRKLRLGILDVHHINEDKFFAFTELLLNEMEDEMDNDVERREYFDNIKLKYSELKRELNNKEICCADQHIGEVVKASDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQF4</accession>
    <name>A0A1E3NQF4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_30297</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019424.1">
        <property type="nucleotide sequence ID" value="XM_019162635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQF4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQF4"/>
    <dbReference type="GeneID" id="30179322"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="459" mass="52935" checksum="D1B1F2C00DFD5AA8" modified="2017-01-18" version="1">MRNIIEQSDREKQKNSFLLESLKQELGKRHYTTPFFEITLNTFPSQKLLFGLKDTEVVYLDYYRTCVSQTISILPKESNFFLSLYLPLAENNKSILYALIGWAGVFLNDESNQHIPLDYIKKSVILSKQELGKEPTKSEKLAIFSLYTILCAVLICAGDVREWYKYFKTLHKFLLDQSNGDLKEIKNCMGDFKETKWLVSNFLYHDVLASISHIEGTCFTMAEYCQILDITMSDPEPVKMIDNDLVCDPLQGCVRPVFLLIGEITNTFVKLKNAEKRLKELEEAYKYEEFSKARNKVYSQLEKKYESLQTLLKNTKPHAMSLCYLKSDKDLEHHLTLFEAFRLSALIYLKTLVKKIPPASPEIQILVVELLPCISIILGSKVQASLCFPLLVAGMSCISKCDRSFIAEKVLQMSNSFPVKNFQRILVIIEHTWKINKEGLKCVYWFEISTKFGWALSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQK4</accession>
    <name>A0A1E3NQK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_13973</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019502.1">
        <property type="nucleotide sequence ID" value="XM_019160159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQK4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQK4"/>
    <dbReference type="GeneID" id="30176846"/>
    <dbReference type="OrthoDB" id="1747771at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="39"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="685"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="702"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="862" mass="98628" checksum="CC73C87AED6E7B29" modified="2017-01-18" version="1">MSIERGQNKRASVIDDDAVAKSSGVDKSKLKRTNRIIRSCSRCKKRKVKCNFEIPCDRCIARNQAHLCTRDPIIFDGLLISNDDTTELKYSQENEVLKRKIKELQDTIVKLKSVKNEQPLKPDKKKVEGPSDIHAATNSSVSSSNGKLSLNDVILNPKNKSQLSHDEHNSSKFNDTDWNTYSTTISLLKKGLANGLILDSTNSSAAEMDYNTEEWLRLSDKNFLKYDAEDSKSKCWLYELDLISELRKPTCDILIKSGLKIVTLFPIIDISDFIREYEEYWADDSITEKHISPLYTKSALNYLFVGLMYALMCFGVYQCDAEVSEQLKFSSYDWDNYSKAFFSASLEALYRGRYMTHPNFKSIQILSILRILCGFLGGNTLSNNLTCVAFFLSYKLDILKSDDPLKHVNMWNTLAYDWYDDHDRYLLSDLDYANSLPKLNRWIDENQELINWPQYYLLFLVNVSIIKRRFYHDSVKSTLKSLKLADIELRYLKVESSRDLDSYSPQQYKNFTRESIKCLNFHVNSLIHHETLDVNLKLSTFLSCEEWSSNYYSTCYLSSCEILKAFISEDIPLEYKSYPSICEHVIYASVFLIVDCMLDAHHMKNYKEVLKLVSQTLPIFQSFKSMVTGAIRGLYTIEKLLILMNLKRKDRSKVSSTTVNLTDKEHDNYKVKKCLNEEVSANEHIKMSSKDAEIKDVQPDSSDIDSSADESSSSDDSDMPLYTSTLENKQKQSFLSMLAQQNSVSQNFQDGDNEKKNFQDSDNEKQIHNFNTFDVPENQNNISNTDYRATNQGSVLLNVNGTSVTPGCSPNILDNTTPQSGMESNATIKTNPPPLIQNTIMDILEDSGWIQFINSIDDLGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQQ0</accession>
    <name>A0A1E3NQQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="10">AK6</shortName>
            <ecNumber evidence="10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="10">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_47397</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="10"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019489.1">
        <property type="nucleotide sequence ID" value="XM_019163128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQQ0"/>
    <dbReference type="GeneID" id="30179815"/>
    <dbReference type="OrthoDB" id="10251185at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000372">
        <property type="entry name" value="Adenylate kinase isoenzyme 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="10">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="10">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="183" mass="21030" checksum="92932B1E26A2B8C4" modified="2017-01-18" version="1">MTFRKLPNIIITGTPGSGKTSHAEEFASQNPEFDHINVSEFAKANDCLEGYDEERDTSIVNEDRLLDLLEPMAEKGGLVIDWHCCEIFPERWIDLVVVVRCNNTILYDRLEKRGYKQSKITENVECEIMQVLLSEAMDNYDQNIVVELQSEDLDSLDANVSRISQWVEMWKENNPDGVSNELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQZ7</accession>
    <name>A0A1E3NQZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019597.1">
        <property type="nucleotide sequence ID" value="XM_019160198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQZ7"/>
    <dbReference type="GeneID" id="30176885"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="54" mass="6276" checksum="D67E3D2CE8F21672" modified="2017-01-18" version="1">MGKKEYEAECAYAQQRETEIVSGNDESFASKRALLSACRRLMSEEKWRKKISTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR18</accession>
    <name>A0A1E3NR18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_31189</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019116.1">
        <property type="nucleotide sequence ID" value="XM_019162680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR18"/>
    <dbReference type="STRING" id="763406.A0A1E3NR18"/>
    <dbReference type="GeneID" id="30179367"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027321" description="Lysophospholipase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="45"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="648" mass="71053" checksum="5C2E3C6A08F091A5" modified="2017-01-18" version="1">MLSFTTLLLTASALVSAQSALNESQIIYSPSEGTCPLVNSSAIYNSTTHEGYIRSNYLISQDEYSYIAARQAKTSDSLIEFLDNLTIPGYNESSFANYFELLNQTNINVGLSFSGGGFRALFTGAGELMALDSRTSNSSALKGLLDSSTYISGLSGGSILLSTLLFNNWTSVESLINDKTTNIWNTTHSPVSRDISFWRELLDEVAPKKKAGYEISMVDIYGRILSRYMFDEDDDSYGLNAVWSDIKYTDAFIDYDIPFPIISATGGVDTNISEYAYNVFEINPFEFGSFSPYVGGFIPIEILGSILDAGLPFQSAHCTFDFDNAGFLTAASSNILEGYQDVLEGFLAGDANDTALAYRAFGQNISASYAEILLDMVNSDINDTLFALVDNPYYNSSLASIDSSMTTNETLKLVDGGYFDESVPLDSFLAPVRQIDVVFAFDNSGQTDDNWPSGDSLFATEERWAESFPDDNFYELPSSVEEFVELGLNKKPVFFGCNGSSLVTDENNPNATVEYNVMKPLLVYIPNTNYTTLSNITDYQFSYAERDAIVANGFEIAQNDGEEDFAQCVGCAIIRRSEERAGIDISPFCNKCFSKYCFEIESVPDNSTISEIIPKTIYSSSALPSQLSSYLSKKTVSGAFPTAFATSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR67</accession>
    <name>A0A1E3NR67_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019166.1">
        <property type="nucleotide sequence ID" value="XM_019161013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR67"/>
    <dbReference type="GeneID" id="30177700"/>
    <dbReference type="OrthoDB" id="3993711at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="434" mass="51038" checksum="7A9CAFB553B0AC0C" modified="2017-01-18" version="1">MLRPTLPLQMVKVGSSGDTVLENMKSKLLHLRNEHHVTKTKETKFEPSRVRYNSEIRNHPHPKDTVTGIYQQPKILKSAHPNNNKVPKSMLRRRDELNKKLSSEQEYQGIHWTPEFATSNLSKTSLQAKSEFDNLIKDWISTNWLNVNPIIQQRPCHILIHDKLLYENISLCETIYKLIIARVPEIAENAPYETVKVAWNNEMDRLLQGRQENLSKLQIERGLREIVKNIYLQFVKDSKTATQKVNSFVKDSFMVRKIISSLYINVKYDEKIFGKLRNIENLNERNDITIIKDNELGFIPSLDYMNLLKKINNKKDNPLQHLNVLKYKSVFGNKINDFQNLQTLKHMLVTKKLHCNEKFIILTGERAISLEYFRLLKLFNKTYANLKLKGCVIYLNRSQALKNKDIIDKKKYTGREMYYVVEEFTDIYDIIEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRA1</accession>
    <name>A0A1E3NRA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_14146</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019712.1">
        <property type="nucleotide sequence ID" value="XM_019160243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRA1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRA1"/>
    <dbReference type="GeneID" id="30176930"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070159">
        <property type="term" value="P:mitochondrial threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000039">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF50">
        <property type="entry name" value="THREONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="53"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="455" mass="52543" checksum="37CA249D74F54EE5" modified="2017-01-18" version="1">MISRLVLPTCSKRFFVRQFCSHDHNPKATKAATTSTATLELATKQQLYMLDPASPGSVFFLPHGTRIFNKLLKFMKLQQEKQGFTEVISPLIYKKQLWETSGHWAKYSEDMFRVEGNDKSKSNELEKDDIYGLKPMNCPGHCVIFKRFNRSYKELPIRFSDWSSLHRNEASGALTGLTRVRRFHQDDGHIFCTHEQVGDEISKTIKLIKICYSVFGLDNFRMMLSTRPDNYIGTLEDWTRAESELSNVLDSELGAGEWSLREGDGAFYGPKIDILLTDHFGKEHQAATIQLDFQLPQRFKLEYQNAEDSYDTPIMIHRAIFGSVERFMALLIDHYAGKWPFWLSPRQAVVIPINDAHKDYAREVVKVLSNESQDFEHATKLSQSKFYVDLDDRNETIGFRTKEAIGKGYNFIILVGDKEIESGKLAIRPSYSRKIENLSVDDTRNKFKDLENNYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRA3</accession>
    <name>A0A1E3NRA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Acetolactate synthase</fullName>
            <ecNumber evidence="4 12">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_30596</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="12">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 12"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019723.1">
        <property type="nucleotide sequence ID" value="XM_019162643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRA3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRA3"/>
    <dbReference type="GeneID" id="30179330"/>
    <dbReference type="OrthoDB" id="16262at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901705">
        <property type="term" value="P:L-isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000008">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000053">
        <property type="entry name" value="Acetolactate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="15">
        <location>
            <begin position="9"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="13">
        <location>
            <begin position="205"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="14">
        <location>
            <begin position="412"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ48610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="603" mass="65473" checksum="026DE2479BF32740" modified="2017-01-18" version="1">MDSSLIGKTGGEIFHEMMERHNVDTVFGYPGGAILPVYDAIYNSSKFNFVLPRHEQGAGHMAEGYARASGKPGVVLVTSGPGATNVITPMADALADGIPMVVFTGQVPTSAIGTDSFQEADVVGISRSCTKWNVMVKNVAELPRRINEAFEIATTGRPGPVLVDLPKDVTAAVLKTAIPISSTIPKKTVSMLSQKELTQYTIECIERSAKLLSKAQRPIIYAGAGVLNSDDGPKRLKELADKACIPVTTTLQGLGAFDQNDYKSLDMLGMHGSGVANLAIQNADLIIALGARFDDRVTGNISKFAPQAKLAAQEGRGGIIHFEISPKNINKVVEATEAVEGDLTENLELFNDLVTSVKERPAWFKLIDEWKEKYPYSYQKETPGGLIKPQTLIQEISKQAAATGKPVIVTTGVGQHQMWAAQHFTWTKPRTFITSGGLGTMGFGLPSAIGAQVAKPDALVIDIDGDASFSMTLSELSSAVQANAPVKVCVLNNEEQGMVTQWQSLFYKHRYSHTHQSNPDFMKLAEAMQIKGVRITKQEDMVSGINEWLNTEGPVLLEVVVEKKVPVFPMVPAGKGLDEFLYFDAEFEKKENELRHKRTGGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRA9</accession>
    <name>A0A1E3NRA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_84678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019729.1">
        <property type="nucleotide sequence ID" value="XM_019165010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRA9"/>
    <dbReference type="GeneID" id="30181697"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="106" mass="11758" checksum="46582E7A9D24C0D8" modified="2017-01-18" version="1">MTVHCGDMTDFSKYESVFEEVSLFKALPSEVIITLDRPVSLCTLEKLTASVIFGDRGVSPSFPERELLSITVTLNMLDIASFVCIGELFYPLNVLVSAKVCDRFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRD8</accession>
    <name>A0A1E3NRD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_19965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019742.1">
        <property type="nucleotide sequence ID" value="XM_019162148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRD8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRD8"/>
    <dbReference type="GeneID" id="30178835"/>
    <dbReference type="OrthoDB" id="10254720at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="10"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="120" mass="13821" checksum="EFB5A67C02D77737" modified="2017-01-18" version="1" fragment="single">GNRHNSSSWCVNCSVTDPLIVEGDNMGKYTLWNVRFKLAKGGHITVKKRYSDFESLHQVLARRYGKQVVVNALPPKNSLFEDRFSGEFLERRRSGLEYWLNSVVLDPLLGYSGEVRRFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRH2</accession>
    <name>A0A1E3NRH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019784.1">
        <property type="nucleotide sequence ID" value="XM_019160270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRH2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRH2"/>
    <dbReference type="GeneID" id="30176957"/>
    <dbReference type="OrthoDB" id="6745403at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="239" mass="27355" checksum="9A5EABB8383F2E76" modified="2017-01-18" version="1">MAKVREQQHLQRCSMTGQNGWTSLSNSEWEARKVNVFDQYGKNINLNTVLEKPIEDENKSKSNNPAMTNPFAQAGLNESLWEGMKGNLLNYLPQPILMFYMSYLFRGYIALKLPFTLTANFKPMFQSSIMTSDLDVSYVTGISWYFVNLLGVESLSKVFGSYFGMPTFFMRPEEIVLENVTLILSSGGAQSQPQQSQQPSFGQPKAEEIFKKQSKEIRIINFKSCLTNVEERFLKKFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRN6</accession>
    <name>A0A1E3NRN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_19328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019842.1">
        <property type="nucleotide sequence ID" value="XM_019162114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRN6"/>
    <dbReference type="STRING" id="763406.A0A1E3NRN6"/>
    <dbReference type="GeneID" id="30178801"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="282" mass="30401" checksum="A51D282A4A44172C" modified="2017-01-18" version="1" fragment="single">VLALVGSGLSAASGLRPYGGGDEDDGDGRDVGAVAWRNYTAIDLATPDAFETNPALVWMFYAYRRHRALAATPNGGHRLLATLSRLSEHVDFLTITQNTDGLHQRAGHAAEQLLEFHGSLFELRCTSFLCSYRSVSRKDPLTPALDVSKYAGDPAAADDDDSGNGDPAATTPLPAVTELAQLPTCPLCTSLLRPGVLWFGEALPLQLLDRADEFIVDHRVDVLLVVGTSHSVWPTAAYLDLVRNQGARIAVFNTVRDPEIDRMAPATPVWQFAGDCAHTLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRZ9</accession>
    <name>A0A1E3NRZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_20032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019983.1">
        <property type="nucleotide sequence ID" value="XM_019162151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRZ9"/>
    <dbReference type="GeneID" id="30178838"/>
    <dbReference type="OrthoDB" id="9936937at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058841">
        <property type="entry name" value="HTH_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040554">
        <property type="entry name" value="KPWE_PEX14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855">
        <property type="entry name" value="CHROMOSOME 10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855:SF1">
        <property type="entry name" value="PEROXISOME MEMBRANE ANCHOR PROTEIN PEX14P N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25871">
        <property type="entry name" value="HTH_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17733">
        <property type="entry name" value="KPWE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEX14-like helix-turn-helix" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxisomal membrane protein PEX14-like KPWE" evidence="1">
        <location>
            <begin position="135"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="181" mass="20613" checksum="4B9952B9907BD507" modified="2017-01-18" version="1" fragment="single">DEVYEQFGSYDFQASPAYKNTLEQVYNQYLIVMSDKDLEVKQDLSNGVFNPSRIPQADREQLQLQTKIFVFCSETENILELQDYQFWLADKQRALGVPQGDAGASAGDDLDVDVDSQMVDSQHDVLAVEPTEEEYTSNYQEIVDMIVNNKPIPGIKQIPKTVLDPTSASESVLQQRRKPWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS07</accession>
    <name>A0A1E3NS07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_13940</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019466.1">
        <property type="nucleotide sequence ID" value="XM_019160145.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NS07"/>
    <dbReference type="GeneID" id="30176832"/>
    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23827">
        <property type="entry name" value="RWD_YLR419W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056890">
        <property type="entry name" value="UBA_DHX29-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF267">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE YLR419W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24899">
        <property type="entry name" value="UBA_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="6">
        <location>
            <begin position="375"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="438"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="627"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="865"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1425" mass="161728" checksum="1C7E858A986A480A" modified="2017-01-18" version="1">MAKKSKKESTPVEEPASDSKKGGKKGSKSSKNGKEEPVSSGKSNTPVKNGRAIVAQTSSWTGKLPGSLLHEHCQKQKWNKVEYDMKKLTDGFIAIPQLSWENPKTKELITIKFHPPKEIVKPQETPLEARHYAATYALHRIAFNKNLHMVLPKNHKDLWSDLEAERKRITADNEKRARLEYANDPFSAVLEKRKTDDAKKKDREAKQASLEKVKKAAIVIGSSPSTTSKTSAVTAKREKSSYAESRSENFSTADADDRLSKVNKVSFPRKVWDNSILFDLDSDIAISIEDAIRNHIEWSEETQKSVEVVDKDDSYAPLLEKIGFRKTHIDESLKYTYTFNDSLEWLIFHLPEDDLPACFAKDDSVSNVKLKIARDLDQERLIAKLVQGGYSRNDAISSLQHNNFDLTKSAVFLTNSLFKDKPPTEKSKTSDSLTEWVEEKNSLSIIYESKIIEDDPPKDDVFGISLNVEGLRPKLLSLKVYRSESYPYDICGMFIVVNDPSYSLPNYIKLSIITYLVEYLYEIGALGMPYIYTCVDWLEQNIMRIINNPGPLYIPPDELTKLKNSRDSNSLSSGDKPALEKKSIKVRKLDTVKIENHYLERVKSSGFEKSIISRSKLPAWKKQSDIVKIINDNRVCLVTGETGSGKSTQIVQFVLDDLNRKKDYQTTMICTQPRRISAIALADRISEERTDRCGKETGYIIRGENKTSDETRISFVTTGVMLRMIQSIFGREKEVQDSFFKNLGYIFIDEVHERSIDGDFLLIILKKMIKNFPNLKIILMSATIDKSIFDGYFGQITTNEIAHVHIEGRTFPIDDFYLDDVLNMTNFKVSRGRASSYFNHDDDLNIVAPSAESKYFQQGNINYELIAELVYRVNEDLDSKNDKGSILIFLPGAMEIKKCLNNIEDDHFWKLPLHSALSSQEQKKIFQNPPHGKRKIIAATNIAETSITIPDAVAVIDTGRVKSVQYDLKSNNTKLVEIWVSRAEAKQRRGRAGRIRNGLCYKLFTKETERKMIPQPIPEIKRTKLESVYLVVKAIGVNNVYEFLQSGLDAPHENNVYNAKRALEDIGALRPNNDELTALGKYLSMLPTDLKSGKVLIYGCIFGCLENSLTLAAIGVTGSPFSCKHEDRSAMKEVQQKFGKDNGDSIAILNAYKDFKSYSSMKERRKFCSDNFLSILRMNDIEATRLQYLNSLREIGFIPFSYDYGKDRNSDEVLRFNRNNDSMSIVKSILTASLYPQLAMVELPDVKYAQSIGGAIALDPEFKKIKYWTRNEYYRKLFDKGELEVSENKSSERIYPATRIFMHPSSSLFINDEKSRVNPSFIVFNDSQETSKLYAYGVTPSSKISVLLFGGSISYDVTSFAGSAKSKGIIMDNWLPIRTWCKNAVLIGKLRILLDKTVRARLEDPRSNAGQDVLKIIEELIKISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSA6</accession>
    <name>A0A1E3NSA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70617</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020150.1">
        <property type="nucleotide sequence ID" value="XM_019163689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSA6"/>
    <dbReference type="STRING" id="763406.A0A1E3NSA6"/>
    <dbReference type="GeneID" id="30180376"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="146"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="774" mass="87959" checksum="57B35B262FF27D5F" modified="2017-01-18" version="1">MAGPYQGSKRHRSPRDAPGRKRVPSLGSSTAKKDSEHLRIGDRNEAKDDDDDDDNLMVLSKEDFENAIKLVTNNKINNVNTWEVKLIEYFYDMNHLKSDDGVSINFQMASATLDGCTKVISKRVDSVGVDTDALIQILAINSDSKSRKKKSRAEDDEDDDNQNDPDYQGSKKSRQDKTGREADKFLSMEKLKFNDRHIQFATIDPVFRKMLADFDEGGAKSLLLNSLRMSNEGRVVFDDTTTTSSASDGHDLSSSVDFTVKPENNANIDDQMETTNGKPTIADVDLKDEEMTIDLLHIYQNVVRDPRFDSLDICHEITEIKSSIEDVEYGKQFVNKINQRMKEDEKKLDLDTPEFNVEYDYDIDNFDLNDNNVDSVNNFNLSEVTNLSTGNSSANPENYKLYNDNGFNANEPFLDEEEVIKKESILMAELDKIPSVRKRQHWKIRAINSNSRIAGENDKESIFTNNEGDSASNSISKNTKSIKEIKKMKKKEYIIDFMNDSTDADTSILFKKAARPLRSLNPDQINSEMTTLPDLKSWSSERLVKSLLKPKRRLRNVFTKRTSSASALYADREFWAAKYNDKTTKPSEEIDQDAADFLYEVMDKPLEENIDSSVNNENAVDEFDGGFDPGVYDFDAPIDDQKLVSDETGPNSQIPGSLGTDVIKAAKPSWHTDSIHFEKRSKKINVRLLKQNLWNVTQQHVTEIKNESSFLSEKEKASDLKLSDIVKDTYKKYQGREKSDLSTSFFFICMLHIANEEGLHIEKTDDLSDVIIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSM7</accession>
    <name>A0A1E3NSM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase eIF4A</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Eukaryotic initiation factor 4A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PICMEDRAFT_70722</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020270.1">
        <property type="nucleotide sequence ID" value="XM_019163722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSM7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSM7"/>
    <dbReference type="GeneID" id="30180409"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000461">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018279">
        <property type="entry name" value="Ribosomal_eS21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00996">
        <property type="entry name" value="RIBOSOMAL_S21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="146"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="327"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="17">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ49157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="491" mass="55012" checksum="5CD8538819452581" modified="2017-01-18" version="1">MENDKGELVELYIPRKCSATNRIIKAKDHASVQIAIANVDEEGRAIPDSNTLYALSGFVRARGEADDQVNRLAQQDGLLNNTKYILTNMSTEATEEKPLTSVDGTIESNYDEVATTFDQMNLKPEITRGVFGLGFEAPSAIQQRAIMPIVAGRDVLAQAQSGTGKTATFSISALQRIDEKEKKTQALIMAPTRELALQIQRVVVSLALHMDVTVHACIGGKSVGEDIEALKAGAQIVVGTPGRVFDMIERGYFKTKDVKMFIMDEADEMLSAGFKEQIYKVFQYLPETTQAVLLSATMPKDVLDVTTKFMRNPVRILVKKDELTLEGIKQFYVNVEKDEYKLMCLIDLYDSISVTQAVIFCNTRRRVEMLTEELTNQKFTVSSIHSDMSQQDRDKIMAEFRTGSSRILICTDLLARGIDVQQVSLVINYDLPDNNENYIHRIGRSGRFGRKGVAINLLADKDITRMREIEMFYNTQIQELPADIGDLFNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSZ0</accession>
    <name>A0A1E3NSZ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_96208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020277.1">
        <property type="nucleotide sequence ID" value="XM_019165113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSZ0"/>
    <dbReference type="GeneID" id="30181800"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="79" mass="9095" checksum="FCDCDE6EAAEA058E" modified="2017-01-18" version="1">MYARAVARLVHNFSHKVFSLHRATGICTFETTLRITSGCGFSRKTVQATQCDVSARRRRHASEEEKWFVRWSSSSSNMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT53</accession>
    <name>A0A1E3NT53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_14760</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020402.1">
        <property type="nucleotide sequence ID" value="XM_019160517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT53"/>
    <dbReference type="STRING" id="763406.A0A1E3NT53"/>
    <dbReference type="GeneID" id="30177204"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000289">
        <property type="entry name" value="Osmosensing histidine protein kinase SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="17">
        <location>
            <begin position="567"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="18">
        <location>
            <begin position="1041"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="377"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="734"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1010"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="742"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="1097"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ49289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1193" mass="133204" checksum="8C5C25E4045243B9" modified="2017-01-18" version="1">MLHKHRLRVSIRTQLIALVLFVAMFSLLVLAIVTGVYFRKVLTDTRVSKLELVAELKSSQFQQMMDSYYFQIYQLSEKQSVQRALSARKAGNNSASALQQAQGVIEQTLESSTSLAYARLYDLSFALVSNVSNEDSVHNATESSLASLFILEQNTSARGPSLPNGLLANGAYLEGPVANDNNYFASLTLPVYTNESILMPQQFLSGYLTVVLDLQAYNGTIASTVKSSEYSSVNYIHTVHAYNNISSYSGFNYAFDNFYLEKNDVDTETLFQISKFPPADDVFYQAKDLGSYVNLINPLGMKVSLGYSSIDLGFALWLVTVEQVEADFERPITKLIKIMVGLCIGLAAFMSLVTFPLAHYSVRPILKLQKVTEEITDRRGLKRRKKRRRHATPFQKLHTKMKSHDLDEKSLSNEDNTEYSEAHQSRALTPQSLSPITPTTPIPPPITPNSGNSFSINNSARNSWCGSLQNPSSMESFSNSYTHDSKISHANSPLPSIVETKGLFYDELTELTEAFNAMTLELDRQYSHLEDRVRARTKELEAAKVQADMARQQAEKANEAKTVFIANISHELRTPLNGILGMTSVAMSESDINKIQNSLELIFRSGELLLHILIQLLTFSKNQLDKSKLQKKNFLVVEVASQIKSIFSKAAKDQDVNLIISLKPNLIRKLILFGDSNRIIQVVMNLVSNSLKFTPENGTVRVLIQVLGPYDEERSKESDYEKVYIKGLPNDAEHYPVPPINKPDDSMDDSKQLSSDVSSIVTTKSSSTLSTLRSSVYIKSLSSLYNSSDCEIEYFNDDEFTKENSTDSYESHHRSRKCFKFGKDNESTGAAWVLRFSVTDTGTGIEQSLQDRIFEAFVQGDQTLSRSHGGTGLGLSICKQFAKMMHGTLTLNSAVGKGSTFTFTIPLPQVGEIILSDSELEEYNKDDFNELYNKKKKVTFIDDDTHVIETDTDTDNSDEKSSKLKRPMNLASSYKSTFTNDNKITQNTKQPENHKDGMIYEKPELITRASTGTAHSYRSKKSDLSTDSFLPTPEENSCDLKLLVAEDNLVNQEVVKRMLKLEGINNISMARDGKDAVEFVRECVNSKNRPFDLILMDVQMPNMDGLSATRIIRSELGYSGPIVALTAFADISNEKGCIDAGMSGFLSKPISRSLLRNVIRQFCPQLCTTVELDENDKNSSDTEQISCSLPTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTB0</accession>
    <name>A0A1E3NTB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin modification-related protein EAF6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Elongation factor-like 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ribosome assembly protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_24181</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020425.1">
        <property type="nucleotide sequence ID" value="XM_019162341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTB0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTB0"/>
    <dbReference type="GeneID" id="30179028"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000006">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000746">
        <property type="entry name" value="Ribosome assembly protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015418">
        <property type="entry name" value="Eaf6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09340">
        <property type="entry name" value="NuA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="20">
        <location>
            <begin position="119"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ49312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1065" mass="119325" checksum="5EA6FD68228E93CF" modified="2017-01-18" version="1" fragment="single">SSAKYKQLVKQLKQNIAKQEKLEDEIDTIEHAIYNKESVYLASTASIIKGFDSYMSASSAHGHGHRREHGRDRDHNYSSLNNGTADSSTLSDAASAAALWSDIMNLSQQKIHTLQQNTDNIRNICILAHVDHGKTTLSDSLIASNGIISRKMAGKVRYLDSRPDEQLRGITMESSAISLFFKTINQKKEISEYLINLIDSPGHIDFSSEVSSVSRLCDGAIVLIDVVEGVCSQTITVLRQAWIDNLKPVLVLNKIDRLILELQLTPEDAYDHLQRLIDQTNTIMASFYNGEIINNHSNWNGDENTWIEHSEEGIYFDPNLNNVIFTSAFDGWGFNLNQFASILSKKTGIDLGEFDNKLWGNFTLDMKNKKVVPISKKSKAKPLFVSFILDSIWKMYNSINDRDTESILKITKSLNIKVSPKELSSKDSKGLTQIIMSQFLPVSSSILSSIIDKLPSPRSAQNIKIDSLLKSIPNSELIDAQLKTDLVECNRSGSVCGYISKMLSIPEEDLPENQPVAVSKDELFERGRIARLKAAKAAELAAKFDSGINNKESVVDDDSTEVSDKVEDEFDLGFEYEEEEEGKLEEEENQNLETLLAFSRIYSGTIKINDTISILSPLFDVSKPYDSEENSKHVHEITVDKLYVLMGRDLTPLEFAPAGTIVGLISSSFRDIYLKTGTIIATDIIRKNKGINFAQSISIIDVPPIVRVTLEPKELRNMDKLIHGIKQLNLADPCVRGYISEGGEVVLETAGELHLERCVKDLEERFAKVDISVGKPIVPFRETIVEDIETELTMDRYKITFAISFWDNWNKDSNNKEDENSQILLENKKYHDILVKANGVTDTSLDGFLKSGFSLAMKEGSLIKEPVEGVKVLIKSIETIEPHFNEGSNETESSESPSIVASKSDQYLSKIRDAIQSSMLKAAPRIKLAMYVVDIQTSAELLGKVYTVIQKQRGSILSEEMKEGTPFFTVKAKLPVLTSFGFSEDIRKKTSGGAIPQLVFDGFETNEQDPFWVPTTVEELEELGEKGDRENWIRSVMNDVRRSKGLFVDEKVVENAEKQRTLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTE0</accession>
    <name>A0A1E3NTE0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_87295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019936.1">
        <property type="nucleotide sequence ID" value="XM_019165028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTE0"/>
    <dbReference type="GeneID" id="30181715"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="67" mass="6881" checksum="3C43EC6437FA1F25" modified="2017-01-18" version="1">MRYCPNAASLAVKYPMGGRVHLPKRASGAVLAFSPANPDGAGEGGGQKGRLAGGLPFWRGVLHVGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTR1</accession>
    <name>A0A1E3NTR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71076</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020644.1">
        <property type="nucleotide sequence ID" value="XM_019163822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTR1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTR1"/>
    <dbReference type="GeneID" id="30180509"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="58"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="169" mass="18688" checksum="72A637A4548EAFA4" modified="2017-01-18" version="1">MLRNVLTLGRSLRSGQRLAITAKNASAQIQLQNQQRFFQMSAAVLHGHLKKPNPGEELHITFITKEGDQKTFEVAEGDNLLDIAQANHLDMEGACGGSCACSTCHVIVDPAYYDKIPEPDDDENDMLDLAFGLTETSRLGCQVHMTKELDGVRVALPTMTRNFLNKDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUL1</accession>
    <name>A0A1E3NUL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_99307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020441.1">
        <property type="nucleotide sequence ID" value="XM_019165149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUL1"/>
    <dbReference type="STRING" id="763406.A0A1E3NUL1"/>
    <dbReference type="GeneID" id="30181836"/>
    <dbReference type="OrthoDB" id="3997840at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="86"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="558" mass="63034" checksum="F6CEE216BC3877AC" modified="2017-01-18" version="1">MSEKKRDTELECPICLDKLTVSDLSYSTTTPCHHFYHTDCILLWTKISASTCPQCRTELSSVNVVNESKIIPIERKKADRLIGMIEQPSSSTDPSSNNPNTSSAASAAASSYGSSRPRIMTTEGLVNVLVSTPENATRRQNRSLYERMIEATSNPIFHLDDNDIDFDDYNEDELYERTSSNSRRSGSLFRRRERSIYTMNEPDTGHNKLSNQQCCICDTSVSISQIIVCPQCSALYHLSCCDGSNCPLCEEWIGDVQAPSIPCASKRSRIISRGADRSLRSRDPNRAKNDDTSYYVELVNELQRRSGNNTFSGTTQNTLPMSAAHSEPEKKTESSTEKEAWEALNLIQQSSTKEDASNDKEIQIPLPTDPVPDYPAERKLKRPKRSISKTLHTQPAKLTESALAHLDSKNDKRPSRKSHCSLSRQKSIVSSTQEGLSFTQKLIVQRLLLKPRLKKDLANKLTFDSYTELNKHLSHELYSYVQHNQLAISSMNAVIELAEREGFLPFVNRKGVDQFSNSCKNNPIITRFVNCEWRNNDESFTGQVQNIINLEVQKWINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDH2</accession>
    <name>A0A1E3NDH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015280.1">
        <property type="nucleotide sequence ID" value="XM_019161996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDH2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDH2"/>
    <dbReference type="GeneID" id="30178683"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF83">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="101"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="540" mass="59110" checksum="41C0D75F0A73F038" modified="2017-01-18" version="1">MSVFTFEVKEGNPVNTETIASLTKELGVEDEKFEDSKDYATLLGVFHDSCEEILKMEDYLPTVDYKRFPRENVHFPSAENNKLGAWGWKFTVKDYQPLLGTRKLLEGAKVCVKDCVAVADIPMLLGTNFITDYVPSTDATVVTRLLEQGAIIEGKAVCENLCHSATSHSAATGVVHNPWALGYSTGGSSSGTAAIVAHEKEFIEIGIGADQGGSVRIPSAWCGLCGMKPTFGLIPFTGCASNEATNDTLGIMTQTVLSNAKGLQSVAGTDGIDDRGFCIKGDTYYDKLLALENPRRLDGIKIGLLLEGFENPAVEERVKECCYEAVKKLEKLGATVESVSIPLHSKGPLIWSGISKVGGYLTKMGCGVGRRGTSLNDLNEKFVTSMHETERWSQAYPSTKNIYFNGMYAVKKFPTLYGKCQNLSRKLKDDYNKVFSEFDILVMPTVPFIARSHCNLKKALTPLDLLGKQVGLSSNTAPFNQSGHPAFAMPCGMLPIQEGPLNDSGVKLPVSLQLVGRWYEELTVYKVAFAFEQSYNWKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDJ7</accession>
    <name>A0A1E3NDJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_74812</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015318.1">
        <property type="nucleotide sequence ID" value="XM_019164879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDJ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDJ7"/>
    <dbReference type="GeneID" id="30181566"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="11">
        <location>
            <begin position="144"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="373"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="437" mass="46536" checksum="26650982B9B0DC8B" modified="2017-01-18" version="1">MSFVTKCHARTVYDSRGNPTVEVELTTDKGLFRAIVPSGASTGVHEALELRDGDKSKWLGKGVTKAVANVNSIIAPALVKEKIPVTEQKKLDQFLIDLDGTPNKGKLGANAILGVSMTAAKAAAALEGVPLYAYLAKLSGSKAPYVLPVPFMNVLNGGSHAGGSLAFQEFMIAPTGASSFSEALRIGSEVYHHLKNLAKAEYGQSAGNVGDEGGVAPDISTAQEALDLIMEAIRKAGYEGIVKIAMDSASSEFYKNGKYDLDYKNPNSDGSKALTGHELGDLYADLLAKYPIVSLEDPFAEDDWETWSGNFSKLPIQIVADDLTVTNPLRIKTAIEKKAASGLLLKVNQIGTLSESIQAAHDAFAAGWGVMVSHRSGETEDTFIADLTVGLRTGELKSGAPARSERMAKYNQLLRIEEELGDKAIYAGKYFATASTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDL8</accession>
    <name>A0A1E3NDL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GPN-loop GTPase</fullName>
            <ecNumber evidence="7">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74818</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015325.1">
        <property type="nucleotide sequence ID" value="XM_019164880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDL8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDL8"/>
    <dbReference type="GeneID" id="30181567"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000579">
        <property type="entry name" value="GPN-loop GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="395" mass="44327" checksum="561EA4CC4E7D5625" modified="2017-01-18" version="1">MPATIICIGMAGSGKTTFVQRLNSHLHQKKTPPYVINLDPAVLKVPFGCNIDIRDSINYKKVMEDYNLGPNGAIVTSLNLFSTKIDQVLSIVKKRSDSFKHCVVDTPGQIECFVWSASGSIITESFATEFPTVIAYIIDTPRSANPSTFMSNMLYACSILYKTKLPMIVVFNKTDVRDAQFAKDWMTDFEAFQDSLRSNAELNDETSNGSGYMASLINSMSLMLEEFYSTLDVVSCSAYTGEGFDDFLKAVDDKVDEYNDFYQKERDRIVQEKKDKEKKDKDAQLTHLMKDLGLKDKKKAASGAADKDEVDVLSDLEDEIERDEKAGYEHGLIDLEDSEREYTFQEDRTGEVRDEEDASEVAKSIQQRYQQAFESTAKTASSSVAEDIAKYINGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDW9</accession>
    <name>A0A1E3NDW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Endoplasmic reticulum oxidoreductin 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_18241</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015433.1">
        <property type="nucleotide sequence ID" value="XM_019161919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDW9"/>
    <dbReference type="STRING" id="763406.A0A1E3NDW9"/>
    <dbReference type="GeneID" id="30178606"/>
    <dbReference type="OrthoDB" id="269384at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017205">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133316" description="Endoplasmic reticulum oxidoreductin 1" evidence="19">
        <location>
            <begin position="20"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="125"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="372"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ44320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="68119" checksum="D99426785CF91F90" modified="2017-01-18" version="1">MKLSKVLSTLTVVYSVCLGVCTGLAGFDFNDIEDDIVVRYEKKPPHHVEFSNYHQFLKTPFKNETYENFLHSYIDESTAVTFQDVNDLNDKIRPVIKNLVTENFFRIFRLNLFKECPFWKGDGFCMHRSCAVDTIDDWEKLPDIWQPEALGKLEDSVLVKKLNADAEYSSKDYCDLDGFIHDTVFVDLVANPERFTGYGGDQSWQIWKSIYNENCFNLGHDQCVEKNFFYKIVSGMHASISTHLSNEYLDTTAMDYKPSLVQFMMRVGNYPDRIANLYLNYIVVLKALIKLETFGVFDDLTFCEDLNFVEKENEFKTRFKELLDPALEFSECQNECLFDENILFKEKNSASVKDEIKQNFKNITRIMDCVHCDRCRLWGKLQTTGYGTALKVLFELTDKESFQLDLSKVELIALVNTFDRLSKSIESINNFKVKYNEAIRKEEMGEDGGDDGFGNDKMGRLNDSAFVAKVNDKETLKSEAKGSLKHSENDYKTPNLFQKETEMYEDVIYPNIGLDDTDGETLGQIFKLELRNVFNTLKFVIRSYYLFPKLVYNWCLIRVVYYWNTFVGHVNEDFDFDRLYRIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEA3</accession>
    <name>A0A1E3NEA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_37330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015578.1">
        <property type="nucleotide sequence ID" value="XM_019163078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEA3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEA3"/>
    <dbReference type="GeneID" id="30179765"/>
    <dbReference type="OrthoDB" id="340227at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07323">
        <property type="entry name" value="LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF132">
        <property type="entry name" value="LA-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="6"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="199" mass="22208" checksum="BE4D11DB939A0606" modified="2017-01-18" version="1">MNWISPESRLTAVNSLAMQLDYYFDISNLLKDMYLRKHMNSQGWINLDFITGFYRVRALSCGDLSVIRDSLDHAGQFEWGVVERDQNATPESSKTKEAVSSEEDSKSELTETEAGSSEAAELANPIANIKIRALAEPLNWVLPESDRDGCGLDDAVPTTIHKREELQNPEVEAEAETEPQSAEPFEQQASSSIPESIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEB7</accession>
    <name>A0A1E3NEB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015588.1">
        <property type="nucleotide sequence ID" value="XM_019161974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEB7"/>
    <dbReference type="GeneID" id="30178661"/>
    <dbReference type="OrthoDB" id="3989831at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="226" mass="26780" checksum="7DA674A309CB7F08" modified="2017-01-18" version="1">MKPTPALLKFFPPVLANIENKRPPLKVPFKYEHVPRFRHKADSRTRIINNMLPDEFKLSNRMLFYSIYFDHDIKLVYKDPKPIDIEQTRILDLGSNLLMLRIYHTLLTNRPETDPIELIDKVDPTEINTLKIRHDFINQFNQILDSKNLLSKISINSTLLKTIKIEKSTRHKVLPMLIGLVHCQYGYEQSKKFIDEWVIRGKWSTVESYSHRGLLEIYTDKKMDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEZ4</accession>
    <name>A0A1E3NEZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_175721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015838.1">
        <property type="nucleotide sequence ID" value="XM_019161649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEZ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NEZ4"/>
    <dbReference type="GeneID" id="30178336"/>
    <dbReference type="OrthoDB" id="5577218at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013635">
        <property type="entry name" value="Ice2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726:SF2">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08426">
        <property type="entry name" value="ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="568"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="600" mass="68996" checksum="ABDA433F584BAB69" modified="2017-01-18" version="1">MQHNRATLSDSLKEETPKRLPNTVPAEAPKQYSSRTPSRSSSTTSLRSSISSTNTLFKTNYALIRCIRVLFSTIYLIYIILTIPLSFEVGGLNCGLSYSLTILILYFCLATLRVLKYHRFISSFLYYLQHLLLPTLLSFFLTIFNSTPRESNSEIIELSSRSILHKIWWQYIIQFWKFFLINSTPLFTILEGFCSLLSVQAIGQLFKYFIKNKSDIWSILNLVLSSCILSASIYFAGKIYVSPLIDLENVGLISASLLGSVFTLTILITFYAIYFNRATTLESSLMVAYIVKCCYELFPELSKNNISSLFKFVFNEFKKIDNQAKQSLTLNKQNIRLSTFIKFFQSTRVAKYWRYYWSNDEITDLSSNIATRFPILVEKSVEFFKIAENFGKILFDFIIEHFPRSFEPLWDFLKISISNLTFSILIQLAYRITVFFAATKIIPILSPMNKVSKRSTNTQQSTSSRLIYLYSPCIIIAVYTNLMIQYNYEIDKETHLFTWIHNKAYELMFSSASSKGRSSLIVTANAASSTLSSFSTTSDSTTMTDSFSTSMPSSLPHSIFTSIHSWQFWNWINIFAVLLLYFSELVSFDPADSALTDHWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF58</accession>
    <name>A0A1E3NF58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Golgin subfamily A member 7/ERF4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_13433</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015884.1">
        <property type="nucleotide sequence ID" value="XM_019160059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF58"/>
    <dbReference type="STRING" id="763406.A0A1E3NF58"/>
    <dbReference type="GeneID" id="30176746"/>
    <dbReference type="OrthoDB" id="5377273at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016409">
        <property type="term" value="F:palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="3">
        <location>
            <begin position="57"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="224" mass="25757" checksum="D0181984D29CDE35" modified="2017-01-18" version="1">MSDTNSNNYNSNASENGPVTTQFYNYHEYHCPYNESLIITHHPNPYPFPYLDMETRVVRIPRLPTVDFPQFSNCLPGLEPVAIRGNEENKKEADGFRFVPSTYTYMNQHFGNTSVSPLSNYIEELEFTGIVDKINAYLYEIYAGTGTNMIWLLANTVLLDLPSIVILLIQKLLGLQLIQAKNFKLEAYVDDLNGTFRDHRRPIRIVQPRESGYLSLDWVIPSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFA2</accession>
    <name>A0A1E3NFA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP synthase subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_60445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015930.1">
        <property type="nucleotide sequence ID" value="XM_019163438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFA2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFA2"/>
    <dbReference type="GeneID" id="30180125"/>
    <dbReference type="OrthoDB" id="67388at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2210:FF:000002">
        <property type="entry name" value="ATP synthase subunit 4 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161060">
        <property type="entry name" value="ATP synthase B chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0138">CF(0)</keyword>
    <keyword evidence="3 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="24848" checksum="D21A43B964635349" modified="2017-01-18" version="1">MLASRIAVRAAARRSVATPVAIRALSTPVEPKEKANSIIAALPGNSILSKTGILATTAAASVYAVSSELYVVNDETILLFTFLGFVGLVAKVMAPMYGEFAKDRTAHVTKLLNDARLGHVSAVKDRIDQVSNLKDVVPTTKALFELSKETAALEAESFELKQKVGVASEAKSVLDSWVRYEAQVRQMEQEQLSSSVIAKVQKDLKDPKFQDKILAQAVTDVEKLFAKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFD5</accession>
    <name>A0A1E3NFD5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17371</name>
        <name evidence="1" type="ORF">PICMEDRAFT_17965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015644.1">
        <property type="nucleotide sequence ID" value="XM_019161828.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015953.1">
        <property type="nucleotide sequence ID" value="XM_019161575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFD5"/>
    <dbReference type="GeneID" id="30178262"/>
    <dbReference type="GeneID" id="30178515"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="52" mass="6048" checksum="FE28016F974A53AE" modified="2017-01-18" version="1">MDQKIRMSSVRIRMSSVRIKMSSVKSVICDIPDEPMARIYDKNFLFSMMGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFI8</accession>
    <name>A0A1E3NFI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pre-mRNA-splicing factor SLT11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17415</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing. Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016003.1">
        <property type="nucleotide sequence ID" value="XM_019161595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFI8"/>
    <dbReference type="GeneID" id="30178282"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="STL11/RBM22-like N-terminal" evidence="5">
        <location>
            <begin position="12"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="279" mass="31577" checksum="E5467067404ACA27" modified="2017-01-18" version="1" fragment="single">MPDVRYDDDSTPAVCDTCLGTNPYVEMKRLRNGAECKICSKPFTVFKWSAQDGNAFSSSRKKTVICLTCARSKNRCQSCLLDLTLGIPIEMRDKLLRIAKIDAAKDLNMSTDIVSNAKNITSRLYNSNLLEEKLKNEEITHLADNEELKRKLEQNLEASLNKQDSKQKQGHSYNKDPISLSKQELLDLLKSFPFNGNLQFRPKNAHIRSFFFFGNGSSLSMFQVKDYFIGLSPEFTKDTVSSLYTQNAGKFGYIEFKTRGIAEKVADIVHGRQKKVLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFW4</accession>
    <name>A0A1E3NFW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_74850</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015356.1">
        <property type="nucleotide sequence ID" value="XM_019164893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFW4"/>
    <dbReference type="STRING" id="763406.A0A1E3NFW4"/>
    <dbReference type="GeneID" id="30181580"/>
    <dbReference type="OrthoDB" id="75343at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="188" mass="19710" checksum="2D7B8DEF4EA7054B" modified="2017-01-18" version="1">MNNPFLPPPPKSMSEMTPEEQGEEGARQIMAFVSSCPGKAMLAGGSGFVLGGVFGFFMASMSYDTPLGIGQMGGAAAAQQLSYLDLPLKEQMKIQFKDVWQRTKSSARNFGYIGLIFSGVECCIEGLRGRSDLYNGAAAGCITGGGLALKSGPEAVVIGCAGFAAFSTAIDMYMRSENGAPPANDYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG51</accession>
    <name>A0A1E3NG51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like protein ARP6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_35931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016233.1">
        <property type="nucleotide sequence ID" value="XM_019162976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG51"/>
    <dbReference type="STRING" id="763406.A0A1E3NG51"/>
    <dbReference type="GeneID" id="30179663"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="47521" checksum="B04EE584F4571B16" modified="2017-01-18" version="1">MAKNVIIDNGQYALKFGNAAQDAPHTVPNCIIRTQDKRVHLGSTLSSQQSLRDLAVLSGGLSGIAFQRPIRKGQCFQWNLEHQIWDNGFIDSKVDSGNGDFLEDSNLLYVESPITLPKFQNMTDQILFEEFGIGNLVRYSAPALAPWLDSFGDNENYKDKETKYRNFQLVIDSGFDATWIVPVIYGIPYYRAIRKLPIAGRFLDGYLREVVSFRHYNIVEEPVLVNAIKHQTCYVAVDYNETLNKLTRLKKNPRELINSELSLSYVLPNNKTDFLGHPILDKSKIQSLLLTDERFLIPELLFHPQLAGVYKAGLIQTIKSSLDAVPELLQPLLVNNIACIGGTSNIPGFAERIVKDLDPEVPVEAQTKVSNYSNITNDTSLLSWYAGKSFFANGGFEKIKMTKADYAEFGAEYAQDKFSFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG59</accession>
    <name>A0A1E3NG59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">General transcription and DNA repair factor IIH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_36381</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GTF2H2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016243.1">
        <property type="nucleotide sequence ID" value="XM_019163001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG59"/>
    <dbReference type="STRING" id="763406.A0A1E3NG59"/>
    <dbReference type="GeneID" id="30179688"/>
    <dbReference type="OrthoDB" id="284275at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000477">
        <property type="entry name" value="General transcription and DNA repair factor IIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="110"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="461" mass="51557" checksum="CB5254D389E5249E" modified="2017-01-18" version="1">MNDDDDDIIVSSSSKRKRNNLNSKASESPHRKNDSGSANGKSNEVKKKAPEALKGASGGYAWEDEYRRSWDIVQEDESGSIETIVSGLVESQKKRYLKNVTPFQRGIIRTMVLVLDYSSVMREKDFRPNRSSMMISYVIEFVSDFFDQNPISQLSIVIMRNGLAQVVSEVSGNPNDHIEALKKLKRIEPEGDPSLQNALEMSRGLLLHVASHCTREILIIFGALFTSDPGNIHGTIQSLVKEKINIRVIGLTARVAICEELCKSTNFGDLKNSYNVVLNETHFKELILDCVTPLAVTKAESENTDGFTLVKMGFPSRVSDTEPTLCSCHSRLVYGGYICPNCKSRVCTLPTVCPCCDLMLILSTHLARSYHHLFPLKLFDEVPVSDHYRSKCCFSCQASFPKGQGETRQLTAAPKTANKMMMSSRYRCSNCEQDFCIDCDIFIHETLHNCPGCESKCTRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGE1</accession>
    <name>A0A1E3NGE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_73971</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016307.1">
        <property type="nucleotide sequence ID" value="XM_019164634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGE1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGE1"/>
    <dbReference type="GeneID" id="30181321"/>
    <dbReference type="OrthoDB" id="1701437at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF4">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="275"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="386"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="442" mass="49878" checksum="DB7736B605C0CC26" modified="2017-01-18" version="1">MSGLLGNPKNRVLQLDGYLLDGQIISILQTQLTEAFADTSLPVGLQRIGSRHGSDLVFLVKVLLFKFFVWDKSSSYGLILQNLKLYDDGKSGKLSRLGKLTLLVHLLASHLIDKLISYSYSDDANELGVTNPTLNFLLKKFTNYLPIMKAGSSFFEVVNRVLFLALGDYTTLYYRLFGIRHESLDTSAISFASNPQSINYEFQDRQLIWNAFTEFITNISDVKMPKIVHRAWKRALHKVMKKEKEKFETGMAIDGIGVNDENSKSMFRFLPERCCAICYADDTSPAKGVDDHLITNPFVTSCGHLYCYYCLIRVMEEARNSDDYEDDDGDDDLVKEEGGIEEGTKWRCLRCNQKVSWSKIYSEKMEELESSVESYFASSAYANLSFSPNVTDEGEEDEEENLSSSSDSDSESLDEMGSESESDGSDGSDTESAYEYDADEMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGE8</accession>
    <name>A0A1E3NGE8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17722</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016338.1">
        <property type="nucleotide sequence ID" value="XM_019161708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGE8"/>
    <dbReference type="GeneID" id="30178395"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="503" mass="57665" checksum="862BCAD9ABB6CE14" modified="2017-01-18" version="1">MKKRDCANSFETLAGFESHNETALVPAFEMNGAIDSTIFTDLENPSELGSHSYFFDSKQKGATNEVKYSFSALESSLELDLEFQALAIISSSDNMKDYFNKYNQCYSLILSLAYSNKTVMYTFAGWLLSINQLPVAEKFLKQSTKLFELTESNLLSSSEDFVSDITGVIVSQTCHALIASSKGDYASWRKIFEKIYKLLDQLGIEEAFGLFQDNPFACWVLGWFFYQDIFKLGRLSNREVFGPLFSKNTYKKLIEVTAYSKIKNPGFPAGCGPLTRCCSNLYITMGEVNTLYDLFAVKLQELNKYKEIFINPIIENKSISRNNLTYFLDSDIYKKYDSMKKHFYSWFNMKGGVLEEKICHTEPNLDIPGLTDDQKNRLNLFHDILKISLQVFLKVRILGCSTANLQIKLLREEIVSGLHKLVAWELNNYLLFPFLIAGTSVHEEVDKLRLKKLYCELKTRLKSGNLEKVWEMIKNVWDSYDDKDKVQNTGDLLSVIDCDVCVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGG2</accession>
    <name>A0A1E3NGG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_12948</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016327.1">
        <property type="nucleotide sequence ID" value="XM_019159990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGG2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGG2"/>
    <dbReference type="GeneID" id="30176677"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF4">
        <property type="entry name" value="ARGININE PERMEASE CAN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="77"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="579" mass="64678" checksum="B8E359962EF7E6EB" modified="2017-01-18" version="1">MTFIRKLNRFKNSLAVEASSGSHFENEADTDVSYIDEKSSPWTSAFDKKYQNTFEHDEDLHGDVLETEVKRGLKSRHVSMIALGGTIGTGLFIGTSAPLKMSGPVNTLIAYLFFGALAYSVTQSLGEMATHTPVAGSFCTFNTRYLSKAIGFSSNWCYWFQWGITFAIEIFSVSQCIMYWTDRVPVWGWILIFFVTITAANFVPVRYYGEIEFWIAFVKVVAIVGFIIYALCMVCGAGVTGPVGFRYWRHPGPWGAGAGLVSNTNTDRFLGWVSSLINAAFTYQGIELTGISAGESANPRKTVPKAINKVIVRILLFYILTLFFIGMLVPYDDPALSSSASFISSSPFLIAITNSGTPVLRHIFNAVILITLTSAANSNVYVGSRVLYSMAGSTAPMIFARTNKHGVPIYGVLFTAVFGALAFLNVSNSGVVVFNWLMNISALAGLICWCFITGAHLRFMKILQSRSISRDTLPFKANFGPVVAWITEFFLVLIVFIQGYKVFFNFDVNDFFANYVSLIMTVVFWIVAQLTYYRREPWLIPTEEVDLDSDARRIDEEVWEDADEDVNVSRFDKFWDTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGR0</accession>
    <name>A0A1E3NGR0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_170574</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016458.1">
        <property type="nucleotide sequence ID" value="XM_019161445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGR0"/>
    <dbReference type="GeneID" id="30178132"/>
    <dbReference type="OrthoDB" id="5358702at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="420" mass="48891" checksum="61B87F8F3C7E52AE" modified="2017-01-18" version="1">MGTLGTRLDPGLKLDLNFTSEFIITILDKKTAARKRIQVLPDEIINESPELSAIMAEGNTHIVSMNDNLTLTHEIVLSKTVFTTIFLESYQYIHRAKELRREVNLVNYQDIKKATFGLLFATSLDTSALRMNLEVLELNNYGKDVLYETYFLVAGYLTCNYEKTNKSSCLWEYFKRLHVWLYDIIPEKSHGAGDMVTFMDEWVSCGGRELLIAFSIKTCLRSLEQHPRNYYACNSLRFFLSKVNTLELREKIFKELIEYAMQNPGDFSLWMVICSSVVDLKTNEFASYRREWKRHSRLESKEKQQDLGNCERLINELYEKMRDFALATGCYGGLLAAFVLNFRLNIWDNLESEIVTTFKDFEAEHNVEINTKRKCLRDCATGQRVDLKNDLLLREDFNLALSMKRAWEDAIAMNMQRKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHB9</accession>
    <name>A0A1E3NHB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein Zds1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_12005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016658.1">
        <property type="nucleotide sequence ID" value="XM_019159864.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NHB9"/>
    <dbReference type="GeneID" id="30176551"/>
    <dbReference type="OrthoDB" id="5589766at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040206">
        <property type="entry name" value="Zds1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013941">
        <property type="entry name" value="ZDS1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28089">
        <property type="entry name" value="PROTEIN ZDS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28089:SF1">
        <property type="entry name" value="PROTEIN ZDS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08632">
        <property type="entry name" value="Zds_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01327">
        <property type="entry name" value="Zds_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein Zds1 C-terminal" evidence="2">
        <location>
            <begin position="1203"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="759"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1174"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="454"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="523"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="577"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="605"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="801"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="865"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="962"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1174"/>
            <end position="1185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1287" mass="143725" checksum="1AA48E3134A39E8C" modified="2017-01-18" version="1">MSISSTSSPRKPVQQDFQAASAAVELEYNAVKRLKRLSIGNSLNFDPDLPLTDSYFLDNKGHDPELDRSTSVLSGHSASSLGRSFDSYLSDDEHDADSSRSGSNASGSTTAYNPRHSDDELVASQGRGAGLQQGHRAPGEADLASLSKLSASSTQHEDIDFNDDSVDFLDPESSFGDSDIIWVPATAHPKLSPENFRKHVQNTVDGIQTKLQRSGSIKSSLNRETSLRDLNRTVRSTKHNTTYRKNGNDNENDDDADDDDDDNDLVQNLAEQDKENRLVTKNKEREKGGLQIRKPSLKELTVQLETLSKRAGLDNNDAVTLARSLSTRSIGFSKLEQEVYSQSDNDDSNDQANNIDQIPNDSMALNRARSRKLLLRNNVNKTRESLFIKDDFFEADGDDFNDSSEIDSDLVEMSFKSSDSGDSFSDYGNSSQPSDFASNLKRGRWTNYRRQQTSSPPSILPNLPNMPQKSRRGMSNNNRSHPHVHVYKDIQKIHNAQKPLGRPQLEPINSKTPSSRLPDLPKSARSASAPTTPSTPASAFVSSNLPLNNHNRAYDDMLKSRNNSNYQQQRQHRHQQQHQQQNRQPYIQQGRQPYPKQQFDHQKRQQQLQQQQYSPYKQNIPQSHSHHQHQPNQKQGQILNSNQDQQHQRKNLTHAQMSPIDKQHNLPKAQNIYRPFNTNQQSPVMSPTDSPDATRINFLNSTNQTSHSRNRYANDKSILDTKRQLVNERVAVINDTKPVSSADGIKSFTNELENQKIRNRSKDSVVGAVDANQSGKGQSHFDGLDPSGPKVANKLTEIDTGIDKGKDKEKEKEKDKEKEKDGKSKTKSSFSSFFKIKRDKPRSVSHATESTEEPKLAFKVKRSLSHSSESGLSSPKADFKSFFGGKKDKDKDKGKEKEREREKGKEAEKEKEKEKEQDKDKEKEKEKGKEKEKSESTSSSIIKKAHDGMSSSPKLGVGLFSSHGNILSKNKSSESVSDANNKKEKTEGSSILGFQHKHKKDNTDIKTKGSNAQNNPSLQHRDSSLTLNKGVERSDSRAIDQISLADESNESTLTNSLEEGEKSKLIQTVLASKIPHSSPGTVSTPTVTSVERDLPSAAKNGLTVVTSNDILTSSGTTDLTNGDPNASAVMTTTMVNQALSLPANESVQQPQAPETTVSPEAESNELIKRSLEETLQQNERPSKPNQPLEMRDSAFGFPLPPVSNSTLVMLDHRFPVQVERAIYRLSHLKLADPKRQLRQQVLLSNFMYSYLNLVNHTLWIQSKEQERNDKEQVGSVVTSTEVTTPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHD7</accession>
    <name>A0A1E3NHD7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016654.1">
        <property type="nucleotide sequence ID" value="XM_019161360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHD7"/>
    <dbReference type="GeneID" id="30178047"/>
    <dbReference type="OrthoDB" id="3994904at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="305"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="610"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="712" mass="80811" checksum="CB05D6FEA86FB7AA" modified="2017-01-18" version="1">MNSTENSDDTGDMIHHGLRQYKEPEQDTDSEQDSTQEVEEFSSFDISDDDVPKSDNQFDEHKEVSPKVFENYETDDERSNLVTADGDSAMNSAPRTPKPDATPSNSKNYTNELHSSFKAADEDTKPSSRLSSDTFETPKTVETRASTMTSDLPSDYSKLMGYGLSLPSASLAKQSKSKLPTKGFKSGSGKTFSKRLSRQTIDDISMDRDSDYNSSAVLQSMDSLFANLSLDDNNHPSNLIPSPNDLVDLQNQLTNCKIQIKLQNDLLRDKIYQSIVDGQNKQELSEELERKIHNSLNSSKLRFQLSHANAEYNSLKEKHEKLLLAIKDLSKSVELLRDQHADLTHNQHEWQRKIDSLILKIETSLNLKKPARITDFNDILETLSSYIATMLDELLESRVAVKASEEHTNKLLETIKAREQELDSIQSDFTKKLQDSEHLNFSYKSKIFDYENLLRSEKSSFSALEAQYSKLQTQYHELENEISNKRKEDEVKSTSRVSQLESQVDNLKKANSALKRSQEGNGPKLDQMIQKVSSHHSAILNSLMTIIDPSSSGNIINACEKLNGDTPYEEIMKVFSVAHNYEISSLNTILKNYQVLVGEKKVHNLQSKTISELQGEVVFLTQKINDLETMKDSETVRIHELQSQNTKLREIVNEKANKVDQLKKLRLEDLKKKWKAAEEALSQTKQGAQMKISELEEELNATKAQLSRYEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHH0</accession>
    <name>A0A1E3NHH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16878</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PRA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016687.1">
        <property type="nucleotide sequence ID" value="XM_019161377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHH0"/>
    <dbReference type="STRING" id="763406.A0A1E3NHH0"/>
    <dbReference type="GeneID" id="30178064"/>
    <dbReference type="OrthoDB" id="63113at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF0">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="178" mass="19865" checksum="08B9040775D55AA9" modified="2017-01-18" version="1">MSNLISTLSSFDVNSISEQLSLDKIKENLQSVKGKFATLRPPQEFFDVRHFSKPSNFSELQQRVTYNLNYYQSNYVCIVLLLSIYALVTNALLMFVIALVVGGMFAISRLQGEDLVLPIGTITTSQLYTTLLVVSLPLGFLASPISTMMWLVGSSCVCVFSHASFMEKPIETVFEEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHH3</accession>
    <name>A0A1E3NHH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homoserine kinase</fullName>
            <ecNumber evidence="3">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_73064</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13986"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016659.1">
        <property type="nucleotide sequence ID" value="XM_019164369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHH3"/>
    <dbReference type="GeneID" id="30181056"/>
    <dbReference type="OrthoDB" id="195231at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="12">
        <location>
            <begin position="70"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="13">
        <location>
            <begin position="253"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="355" mass="37985" checksum="0C3248F74820F5B8" modified="2017-01-18" version="1">MTREFTIKVPASSANIGPGFDVLGIGLNLYLTLSVSVNEASTASTKDPYNAELSYSGDGSGSVPLQSDLNLITKTALYVLHANHVRAFPPGTRIHVNNPIPLGRGLGSSGSAIVAGVLLANEIGSFGFDKNRCLDYCLMIERHPDNITATMMGGFVGSFLRNLSPEEETVVHGDLGRILPSPNTSADYVPPNAPLDLGTHVKYPWNPVIKCIAVIPQFEVKTEDSRSVLPSSYSKKDVIFNLQRLAVLTSAVGDLKPAPEVIYNSMQDSLHQPYRKVLIPGLTEILSSFTPQTHDGLLGICLSGAGPTILCLATKNFDAIATDIIAQFKSHNVACEWKLLEVASEGAVVEQGSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHH5</accession>
    <name>A0A1E3NHH5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_177214</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015906.1">
        <property type="nucleotide sequence ID" value="XM_019161707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHH5"/>
    <dbReference type="STRING" id="763406.A0A1E3NHH5"/>
    <dbReference type="GeneID" id="30178394"/>
    <dbReference type="OrthoDB" id="10266058at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="37"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="63005" checksum="AE2464CB594E6EBE" modified="2017-01-18" version="1">MKFVKCIAETWTEETLYSILKDQAVGTSLATMFNERRAANRQSARAGPSNNSNKEEKGLERDTRRTREKDRAFKPERRYGENYRKRSSEFDSPSKRPPPEGGRYRSEGRNGYYDSNYGQQGYGNGRRYGGRPNERGTPRGRNDAGPIVPLDSLGMTNSQWDKYPAKLKGMSARFAKSAGVFSSRSESSLEQLTEENIVNLIKQQNNLLKMRKSRLGTQRIMGPTESKLSKRVIVKGVDFDKVAPQSVQKFLESFLASVVIPDVSYEDLTLTSTVEGGTLVIDCTTSLVATTLVSFNSKHIPELEANLIVERPGDYIEIADSQNQEESLPDVEVLEQIVETSGLCCANNIPEDMSNGKFKEILQKYGNLRSAILLIGKVTYECCGVAFFSFYSTKKSIDETIEDINKEEKWDCFLACKNQTNEYSQQCDIKVANVKDFIDTKSKDITKHKVTSTIQLLNSISIEDALDDTKYNQVKDAFEEELSQLSGYEKLVLVRPSQGFRIQQIDEVQPEFGRILVTFKTPKDAEACLNKVCGRYFHGRVIMGGFLDDLDYRDVFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHH7</accession>
    <name>A0A1E3NHH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016694.1">
        <property type="nucleotide sequence ID" value="XM_019164379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHH7"/>
    <dbReference type="GeneID" id="30181066"/>
    <dbReference type="OrthoDB" id="10343074at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="273" mass="30175" checksum="59C4BF87344BDA20" modified="2017-01-18" version="1">MTQFEKNPSASNTAYKACDPNLNVPIFADQVHHNSGFSLVDDENATNFSINLSDDNDENRIVQSTLTMEIDPIEQLLMSTSASFNEFENDFNNPFGPSTFSDSNQLYYPQAGVVNSSPTYNHPITMDNYFVDNEAIYQMYGDTDCNSLHDYSSISNIIYTKKIKEKLRRKRHASSADLTVTTTSDPAKPPKVFKSPSTLSLANSRRVNLRSKSLGAISSQKNINTTSVKAGSADPIETFPLPTGKISGNSSPTNKATNPFYHPPEILKRLANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHM8</accession>
    <name>A0A1E3NHM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_68884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015956.1">
        <property type="nucleotide sequence ID" value="XM_019163510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHM8"/>
    <dbReference type="GeneID" id="30180197"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="69"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="306" mass="34111" checksum="F8D2A65F7E8E6289" modified="2017-01-18" version="1">MTTQTAVHAALKLQPVETRKEEKQENVNLRGYNTEVKDGVNNIAMGIYKHPYRLPKITDPFIKREWLLNLMAGAFRVFARKGFCEGPAGHISVRDPIDPDTFWINPLAVHFSLLTPQHMVHVDEYGNVIGGNRATINAAGFKIHSTIHKARPDVNAICHVHSRAGNHLDMLNQDACIFYKNHTVYNDFGGVVLESQEAKHIAEAMGPTNRVIILKNHGLLTAGTNVGEAAYLLCLMDTYCEVQMQVDSVQPSGRYRRDVIPDQEAKYTYEMTASAENLYSSFCADYEVEVVASNGAITSATSYHTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHQ1</accession>
    <name>A0A1E3NHQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit CNL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CNO-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73194</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1), a complex that is involved in endosomal cargo sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BLOC1S4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016793.1">
        <property type="nucleotide sequence ID" value="XM_019164410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHQ1"/>
    <dbReference type="GeneID" id="30181097"/>
    <dbReference type="OrthoDB" id="5424991at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034455">
        <property type="entry name" value="CNL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39145">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT CNL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39145:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT CNL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="110" mass="12514" checksum="8CE6D8942A924C9E" modified="2017-01-18" version="1">MTLSTSEDAVGSDPLDVQTLAANFDTLMANINKRTQQLAEQVYEHVQEKKQISDENLTAIEAKLQQFGGLLDKCNDLNTEIDKLEQLQLFTRDFNLRLQSLSDTLRRVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHR8</accession>
    <name>A0A1E3NHR8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_54292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015996.1">
        <property type="nucleotide sequence ID" value="XM_019163176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHR8"/>
    <dbReference type="GeneID" id="30179863"/>
    <dbReference type="OrthoDB" id="188035at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016833">
        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640:SF5">
        <property type="entry name" value="SODIUM_BILE ACID COTRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 10 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13593">
        <property type="entry name" value="SBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="465" mass="52880" checksum="B624EE62856E834B" modified="2017-01-18" version="1">MRFHIPSLSIDDRIDALEKTSPRKYRVYSFLWRTIWLGYWFYIVLAIFIVIARFAPNFARHGGLIRGQYSIGYGAVIVIFLGSGLSMQTKQLVENVLHWRAHLTVFLLQFLVTSSIIFGFACAIKRANNPIISEWMLVGLIVTGCCPTTVSSNVVMTRKVDGNVLLTLCEVFLGNLAGAFITPALVQMYCQGEWEFANPANGSTINDVYRNVMKQIGFCVFLPLFCGQVIQNVFPQQTKWFLVTFKFNKVGSFMLLLIMFSSFSTAFYQHAFTSVSHQSIIFICFFNFGIYLFFTAICFIFSRPLFLMYFFKKDPEESTSKVYKFFYRLVRPFYYNRADTVSVLLCGGAKTAALGVTLVSSQYGDHNPHLGELLVPLVLYQAIQVITAGLLTPFMKKWIHAGPDYKQQHQDKSSPCDEETGKPDNNAEVSEELNDDSQGTSEDSYHSNNNSDHSDYNKATSRYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI40</accession>
    <name>A0A1E3NI40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_12214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016869.1">
        <property type="nucleotide sequence ID" value="XM_019159899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI40"/>
    <dbReference type="GeneID" id="30176586"/>
    <dbReference type="OrthoDB" id="3993274at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="290" mass="33147" checksum="F38BFB3FDC7779EF" modified="2017-01-18" version="1">MLLKGSVLNRSILPKRIWSNGCLKRSISSSAPANTKYDKKKSTVKGKSRPKELSYEQESEHYLLKELKKEDAKYNARNQQTSNIVKQFADVLNLYGLKIVSAPLRGHTNLPYDYIHLSKKSNQYSTDILLDTRCTGINDAQLVVTDETNESQVLFLNGNFTRERGFVFYSAMIIDTKEIGKSSSEVCHAICSAEEWIFRALNNFYEKKIDIDHPEVARFLTFQHEECNIPDLTGSMLVDALLHYTITDLINKNPEEFASLNSILDMLVSYCHDSAYGNWIQCFKSFINSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI44</accession>
    <name>A0A1E3NI44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Choline monooxygenase, chloroplastic</fullName>
            <ecNumber evidence="5">1.14.15.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_17062</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the first step of the osmoprotectant glycine betaine synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>choline + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = betaine aldehyde hydrate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:17769"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15870"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.14.15.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (monooxygenase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the choline monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.7" evidence="5"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016904.1">
        <property type="nucleotide sequence ID" value="XM_019161448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI44"/>
    <dbReference type="STRING" id="763406.A0A1E3NI44"/>
    <dbReference type="GeneID" id="30178135"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="UniPathway" id="UPA00529">
        <property type="reaction ID" value="UER00430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019133">
        <property type="term" value="F:choline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019285">
        <property type="term" value="P:glycine betaine biosynthetic process from choline"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00680">
        <property type="entry name" value="RHO_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03469">
        <property type="entry name" value="Rieske_RO_Alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.380.10">
        <property type="entry name" value="Naphthalene 1,2-dioxygenase Alpha Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015879">
        <property type="entry name" value="Ring_hydroxy_dOase_asu_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001663">
        <property type="entry name" value="Rng_hydr_dOase-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43756">
        <property type="entry name" value="CHOLINE MONOOXYGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43756:SF5">
        <property type="entry name" value="CHOLINE MONOOXYGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00848">
        <property type="entry name" value="Ring_hydroxyl_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00090">
        <property type="entry name" value="RNGDIOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="13">
        <location>
            <begin position="53"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="447" mass="50978" checksum="2DDAD2D7FCE1C5C6" modified="2017-01-18" version="1">MATVVEKVTPAAPPPDSAADKLLPGRHTLPASWWTSEKVLDFEKRAIFAKSWLFCTHESRFKKAGDYFAFNLLGWNFFLIKNKEGDIKAFHNVCRHRAYPVVRKQQGSSIILGCKYHGWSYDTNGLLKKAPHFDNIEGFDPVANSLYEIKTHVSRQGLVFINFDNTEDAVPFEDFFAGLESEMDEFDFGDYEYHMSYELDGQFNWKTLMDGYQECYHCPTAHPGLNLAFRMETYKVVPKGNYCRHYAKIVEEDIPAVVEENDPDTESSWFGFGKKKEVSKAPKKQNPGGSFNGLWCYLFPTNGVNCYSPAWYSIRVLPITAKRTILQYDIYTKKGATADAKKEFVDFLQQVELEDFNLCEATQKNLNNGVYSTGYLHPTKEKGVLHYQGLVSDMVKKHFALEKETGRLINPAALTARQSEEAEELEKICTGIECLGSNPEDHDGLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIQ7</accession>
    <name>A0A1E3NIQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Plectin/eS10 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_35319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017059.1">
        <property type="nucleotide sequence ID" value="XM_019162934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIQ7"/>
    <dbReference type="GeneID" id="30179621"/>
    <dbReference type="OrthoDB" id="5211809at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000025">
        <property type="entry name" value="40S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005326">
        <property type="entry name" value="Plectin_eS10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037447">
        <property type="entry name" value="Ribosomal_eS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03501">
        <property type="entry name" value="S10_plectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Plectin/eS10 N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="122" mass="14506" checksum="B2F809CD82242C83" modified="2017-01-18" version="1">MIIPKADRRQIYMTLFKDGVLVAKKDFEIQHDELATKNLYVIKAMQSLTSKGFVKTQFSWQYYYYTLTDEGLDFLREFLGVPEGIVPQTLMEQPIPQRQQNFRREGGRRFDGDNGYRKRNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIR6</accession>
    <name>A0A1E3NIR6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_150978</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017150.1">
        <property type="nucleotide sequence ID" value="XM_019160662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIR6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIR6"/>
    <dbReference type="GeneID" id="30177349"/>
    <dbReference type="OrthoDB" id="268521at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_uL16_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_uL16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220:SF13">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="213" mass="23942" checksum="D36D20791E33DF8E" modified="2017-01-18" version="1">MFASVSRLFTPFTRVAQPSAKRYGHELAPRYKQVMKKQKGRVSVRTGGSIKGTTLEFGTYGMRLKSEGLRLTAAQLQAADNVLTRFVKKGNGTMWKRLCTNVAVCIKGNATRMGKGKGPFDHWAARVPTGKVVFEVDGMHRHAAKDSLRRACEKLPGIWEFIDKDTPARSGFAYVEEQAKVNYLEEAKENPTREFSNFLKSKSPEFTKFTGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIS6</accession>
    <name>A0A1E3NIS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73487</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 22 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017123.1">
        <property type="nucleotide sequence ID" value="XM_019164495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIS6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIS6"/>
    <dbReference type="GeneID" id="30181182"/>
    <dbReference type="OrthoDB" id="203279at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.160">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12623" checksum="B388DA9B93244A12" modified="2017-01-18" version="1">MSGGSQVSVMSKFDNNIQLILTKFNEVLELLRLDGKDLETQAVEAIQIQSDTQIVVRLVEELLHLTKSLKEKWILGQVHRKSRDVLADNNYELYVKLNGILSDITEAAEGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIU8</accession>
    <name>A0A1E3NIU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hsp70 nucleotide exchange factor FES1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Hsp70 nucleotide exchange factor fes1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17291</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FES1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017172.1">
        <property type="nucleotide sequence ID" value="XM_019161533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIU8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIU8"/>
    <dbReference type="GeneID" id="30178220"/>
    <dbReference type="OrthoDB" id="10250458at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF18">
        <property type="entry name" value="HSP70-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="309" mass="34431" checksum="045860D1D60F6333" modified="2017-01-18" version="1">MEKLLQWSTAQQSQDPELRAKAPAPDPKLLAQVLGADTGKDDTTLMKEDISVLVCNDPQISVDDKLTALEDFEILVQNLDNANNISPLGIWPEIAKLYTYEGEEQDEFRGLGALITGTAVQNNDKAQRDFLKSVGMEGMQRLLDLTSKENGFNVRARALYAISSLVAHNGLLYGIFVKTNGWKRLEGILSEDFCNDKKDNKVLLRSLSLLKCLLYDEITQENEAVKTSKEDRFSEAKSCGAFMTIIKKLSPDSHVEVNERIVNTLSYAALNKYTFSPEEISAMKEGLNKLSSAKITVDKDDLATLQKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ60</accession>
    <name>A0A1E3NJ60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17350</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NADPH-dependent formation of L-aspartate 4-semialdehyde (L-ASA) by the reductive dephosphorylation of 4-phospho-L-aspartate. Mediates the second step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:24286"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017263.1">
        <property type="nucleotide sequence ID" value="XM_019161557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ60"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ60"/>
    <dbReference type="GeneID" id="30178244"/>
    <dbReference type="OrthoDB" id="1894490at2759"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000200">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000016">
        <property type="entry name" value="Probable aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="14">
        <location>
            <begin position="5"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="13">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="358" mass="38679" checksum="9CED9E7D9AD48952" modified="2017-01-18" version="1">MAMKKAGVLGATGSVGQRFILLLSEHPEFELVAIGASARSAGKKYVDACTWKQTDLMPAQVEGLVVSECKAEAFKDCDVVFSGLDADVAGPIEKELVESGLVVISNAKNYRRDPNVPLIVPIANSEHLKMIENRVAKADCKQGYLICISNCSTAGLVAPLKPLVDQFGPLEFVSTTTLQAISGAGFSPGVPGIDILDNIVPYISGEEDKIEWEAKKILGGLNEDATEFQLDDDLLVSAQCNRVAVSDGHTECVSFKFRNRPEPSLEQVKQCLRDYVCEAKLLGCHSAPEHAIHVLEQPDRPQPRLDRNRDAGYGVSVGRIRPDPIFDYKMVILSHNTIIGAAGSGILIAELLAAKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJT0</accession>
    <name>A0A1E3NJT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_26740</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017449.1">
        <property type="nucleotide sequence ID" value="XM_019162462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJT0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJT0"/>
    <dbReference type="GeneID" id="30179149"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031138">
        <property type="term" value="P:negative regulation of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000047">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000120">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF8">
        <property type="entry name" value="RNA-BINDING PROTEIN MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="9"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="100"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="233"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="324"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="413" mass="46291" checksum="EB3E42776899348B" modified="2017-01-18" version="1" fragment="single">AEISSVPSRTVYLGNIPSGITPYELLNHVRSGTVESLKMISSKNCAFISFLDEKSATLFHSDAILKRLTINNHDIKIGWGKPTPVQPIVASCVSRYNATRNVYIGNLPENTSEKELANDTKEYGEIDTIKMIHEKKIAFIHFTSILAAIRCVQSLPLIEKYKDRRIFYGKDRCAFITKTQQHNAAQYLGLNPNTENFLSQVDRDLIANALVQQSNAAAMIATTTGGTSNLGNRTIYLGNLHPDSTIEEICNVVRGGILQNVRLINERHVCFVTFIDPISAAQFYAMSSLHGLIIHNRKIKIGWGKHSGPLPNSINLAVGNGASRNIYIGGLDKIPEIQFTKDKLYYDFKEFGEIEQINFFQERHCCFVNFTNISNAIKAIDGIRNNPDYQRCKINFGKDRCGNTPRQFQLNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK30</accession>
    <name>A0A1E3NK30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017031.1">
        <property type="nucleotide sequence ID" value="XM_019161495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK30"/>
    <dbReference type="STRING" id="763406.A0A1E3NK30"/>
    <dbReference type="GeneID" id="30178182"/>
    <dbReference type="OrthoDB" id="2573941at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="29"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="139" mass="15530" checksum="F6C997E26EC27CE7" modified="2017-01-18" version="1">MSVQKLNAVELQQGTAASASWHRDYADTNTVYVGNLPAELNEHDLLILFSQYGVPTAVRLARDPQTGRSKRFAFLTYEAWESTVLAVDNFDSWNVTPQHRLRVNHAYYKGRGGGSGAGDDEAARWERAVQAELLDKDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKX2</accession>
    <name>A0A1E3NKX2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_11771</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017915.1">
        <property type="nucleotide sequence ID" value="XM_019159838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKX2"/>
    <dbReference type="STRING" id="763406.A0A1E3NKX2"/>
    <dbReference type="GeneID" id="30176525"/>
    <dbReference type="OrthoDB" id="413582at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF508">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="6"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="342" mass="38367" checksum="E6F6CD1020DA093C" modified="2017-01-18" version="1">MDALGWKPLAKIGGGRFSDIYRVLKSDSENNSANNEEQQLFALKLVDPDDEKPPHNIRNEIKILSDLKNAKEGQDAVIPNVINLISVSSNNIEYGLLFPLLDMTLNHVIKSHIKSRTIFNSDGTMKMRKVNTIPIDSAFKIVVGLMKGLDWIHKNGVIHRDINPNNILFGRENPEMPVIIDFGISYQEPYNNGLEKPNKKFTDIATGIYKAPELLLSKRDYSNKVDMWAVGILITVILSKDGKPIFEQDSMYSDLVLLSNILATFGSPPSDWSDCKGLTSFDSLNRTFFTKSPKPLEEIVPRLFEEPETLSSSKLQKTITGLTKYETSERLSAHDALAILLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLS9</accession>
    <name>A0A1E3NLS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leo1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018197.1">
        <property type="nucleotide sequence ID" value="XM_019160653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLS9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLS9"/>
    <dbReference type="GeneID" id="30177340"/>
    <dbReference type="OrthoDB" id="20844at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007149">
        <property type="entry name" value="Leo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146">
        <property type="entry name" value="LEO1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146:SF0">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN LEO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04004">
        <property type="entry name" value="Leo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="46175" checksum="C3C6AB8136A5BE63" modified="2017-01-18" version="1">METEIEAQTSVPDRVSVLEKETVVGDAGEEEMDDLFGDDEAEEEGGTGSGVDVGIDEDEEDEEVEGVEAGDAEEAEEAELKHADLTVDRHPRSHQPKDGHAYSFPLPRFLFVDPAPFTPNVFEQQLKEFAASTSLNGADVAESTLKSSVQFKKLQLTNTVRWRYAKSKTNELFKQSNANIVEWEDGSMSLKLGHEYFDVKTKPNDDNLLAVRSGQALVSLMNMDRSVQILPPSMKSRAHQILADTLTKNMKMKKSKRINTIVTSEDPELKAREVERAHREIEKARRRQQQKLQIEEERSERHSGSGNGARFRDAGDGYDDDIDIADEGDEYDDDDDDEYGAVPGAKNGFVVDDDDEGSELDDDELDRAAERLKKVKRDGAARYRDAAADDEDDEDAVVRKKRKVVLDDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLZ1</accession>
    <name>A0A1E3NLZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018234.1">
        <property type="nucleotide sequence ID" value="XM_019163866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLZ1"/>
    <dbReference type="GeneID" id="30180553"/>
    <dbReference type="OrthoDB" id="188042at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009291">
        <property type="entry name" value="Vps62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053102">
        <property type="entry name" value="VPS_Associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48220:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06101">
        <property type="entry name" value="Vps62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133419" description="Vacuolar protein sorting-associated protein 62" evidence="1">
        <location>
            <begin position="24"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="52796" checksum="5F91FF5B73EB9FD2" modified="2017-01-18" version="1">MLLLQLFAVLSFLFQTCIPTCQASPIDRLSEKEVEDRLDALLQSRASYKNLPPLEAYPSPESRTLKPGSIPEYVAKYAPLVFLYSEEKYLPYDIEEYVSHFRPTWRNGTVIDLPGKDRNDPLYKNQVLHLSDLENLPQSTHEELVFLSSLSDFDVDPEWITGVHNKPNYYNGEIKDAPVTLIVTDKGKGWVDAYWFFFYSFNLGPFVMGFGPFGDHIGDWEHCLVRFYKGEPVIIWMSAHGGGGAYFYDHMEKETLDSSGIAVGNGVQPVIFSARGTHANYPSTGQHSHDLPYSILSDFTDRGGLWNTALNYLAYTYTPADDNLSGEAAGMELSLANGSHPFRESKYGKWLEYNGAWGDPKLEPEDPRQHWSPFEWKYIDGPTGPLTKNLVRTSPCQRAKWWNFWNGCNVRRYIQYGQGVFDQEGNNSCGSLYVHFKNKWVRKLVEVVTWGGWVCFSIDLVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM44</accession>
    <name>A0A1E3NM44_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_108790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018328.1">
        <property type="nucleotide sequence ID" value="XM_019159724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM44"/>
    <dbReference type="GeneID" id="30176411"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="119" mass="13845" checksum="85E9D1E4A6F65813" modified="2017-01-18" version="1">MFVRGGVCGGRNFQTAESCAALRTLKYCVREQIGSFQCTPVFAHYTRSGKRGRRKCLRHQGAAAAKNFRRSTQKALIRVDRRKFGRDRRVGDGPVTEARPGRPGCRERRFRWWRRVQWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM47</accession>
    <name>A0A1E3NM47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the free Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018294.1">
        <property type="nucleotide sequence ID" value="XM_019160698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM47"/>
    <dbReference type="STRING" id="763406.A0A1E3NM47"/>
    <dbReference type="GeneID" id="30177385"/>
    <dbReference type="OrthoDB" id="15735at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000008">
        <property type="entry name" value="GAF domain-containing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000614">
        <property type="entry name" value="FRMsr_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF15">
        <property type="entry name" value="FREE METHIONINE-R-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01320">
        <property type="entry name" value="UPF0067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="61"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="172" mass="18614" checksum="7049F05BD8927529" modified="2017-01-18" version="1">MSHHADYSNFESAASRKDALETLVMSYEALAASQRNWVANLANCSSLLYHCYSDAGVNWAGFYVVDKDGDAEQLILGPFMGKVACQTIRIGKGVCGTAAATLQTQLVPDVEAFPGHIACDGETKSEIVCPITQEGRLIGVLDIDCLNLNGFGDVDKHYLELLCRKIAETCSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM98</accession>
    <name>A0A1E3NM98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_10274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018375.1">
        <property type="nucleotide sequence ID" value="XM_019159655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM98"/>
    <dbReference type="GeneID" id="30176342"/>
    <dbReference type="OrthoDB" id="4080905at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="345" mass="39359" checksum="536968ED276A48C3" modified="2017-01-18" version="1">MSITIAQCAVRLKSNPSLCHNLKKCCHHSHFTPLSPLRKFATSPPFLQGTNKSDQNAKNKQKIAKSRLNLPPSFRSSHPLPKNQVTDFEREKIQLENNSYDSGQKIPPQPIVYKNPWWTPLLKLGVVFVPFFVVGIGYVAYETYNDRAVFFPLWINSSVPLSHAAGFENVDVDMLKEVAKTNLLRRLNVNHEIREYFGLPITLSDYELFDVRIQYNKLAVEGVELDFRKSWLKPIIRYREIDTPVLPNNVNKYVQPLKARVGGGVDEDPEQDSIFAKDMEYKIKIRATIKVANEVLHRIEPGSGRITFDAEVELDHTRLMKITSALMHFRNKGGSGSGGTLEKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMA1</accession>
    <name>A0A1E3NMA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15241</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018372.1">
        <property type="nucleotide sequence ID" value="XM_019160732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMA1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMA1"/>
    <dbReference type="GeneID" id="30177419"/>
    <dbReference type="OrthoDB" id="2104723at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="7">
        <location>
            <begin position="74"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="306" mass="34121" checksum="7CF984E1211F9B31" modified="2017-01-18" version="1">MFTTITPVQNILSISSHVVHGNVGNDAIQFPLNLRNWNVDCINTTNLSAHPGHGVFTGGKEKAKLVGDLFQGLKELLLDDEYQAVIIGYVASSENLQVIWNDILTQFTRKNVVKVMDPVIGDNGKVYVSQEIVDLYLELLGRNEIDIDLLTPNMFEIEVLTGIKINSWETVKKSIDVFCAKYMRVKNLVITSVTIDGKMYCVGSSKGKSFYYKVSEVDAIFSGSGDLFLALLTDELVKNEQVLAVSVCKTINVVTDVLNMSYQLLKDDCTTKRNANGKLYIPDLKLVESKNILVRDVEVIEPTYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMC0</accession>
    <name>A0A1E3NMC0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_76353</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018364.1">
        <property type="nucleotide sequence ID" value="XM_019164948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMC0"/>
    <dbReference type="GeneID" id="30181635"/>
    <dbReference type="OrthoDB" id="3996548at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133416" evidence="2">
        <location>
            <begin position="21"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="461" mass="53260" checksum="60ADE7B52C5EA785" modified="2017-01-18" version="1">MVKLCYTFPLVCVCVLPTLALSTEYVKHNSRQVVLSKPNQEYIKKSYSLIEPHLQKRNPSYRVVKTKVSSVTKKNDDIVEDEEFEEDETAVNSYSFRSLQKKLGLGDKKFEMPVSGPVPDWDDQYYDNDGKPIVYQVDTEELKELMDNYLEMGKEKWAAFLATSECNKFQKLMDKLHNLFKKNENADDNDEDDNEDDEDSKAQGPFGKYENDADDEEDNDDDDRSSRNNKKVSSAANKLSLKDLKGLKNVNSQPLFSSIPEEEIEEFHNLLADVVSYWEEEDAISILEAASGLSYKDFDLFEEDENPKKKKTAKRIKPSLSVIDDNDDDDDDDEGDDDDGDINANVNDDDDENDDNNEIKQHQSSQPDLLGKIDPKLLKTKAQKDWLKSNALRRANMIQKLMDGQLDEENYSRQDEVRYRENNIFLEEDENEIPNTAFRLQSTNLAMYVFFLVEALLFYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMN4</accession>
    <name>A0A1E3NMN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71456</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018495.1">
        <property type="nucleotide sequence ID" value="XM_019163929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMN4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMN4"/>
    <dbReference type="GeneID" id="30180616"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="56"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="548" mass="60545" checksum="C90209F8C4D7E52D" modified="2017-01-18" version="1">MSSSSLSEKPANNFEHLNSPEEESSDEQHNYDAAVAAQANVVPNTSKSKYVMPAFLCLMIAFGGFVFGWDTGTISGFVNMPEFQHRFGQIGGNGQYYLSKARTGLMVSIFNIGCAIGGVTLGKTADMYGRKKGLMITMVVYIVGIIIQIASVKSWVQYFIGRIISGLGVGAVSVLAPMFISETSPKAIRGSLVSMYQLMITLGIFLGYCTTYGTYHGYVDSRQWRIPLGLSFAWALLMIVGMVFTPESPRYLIEVGRIEEAKASLAKVNKTEADSDFVLSELDVLVAAIELEQSAGNASWGELFTGKPKIFYRLMVGVFLQSLQQLSGDNYFFYYGTTIFKSVGLEDSFETSMVLGVINFASTFLALWVVDHFGRRKTLMGGSAGMLVCLVIFASIGVKDLYIDGYDGEARKPVGNAMIFLACFFIFFFATTWGPCVFVVVSETYPLRIRSKGMAIASAANWIWGFLIAFFTPFITNAIHFAYGYVFFGCVLFSFFFVFAFVPETKGLSLEDVDELYTNYTPGLAFMNSFRKEKLEEKHADLDNSPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMU3</accession>
    <name>A0A1E3NMU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRM5</name>
        <name evidence="12" type="ORF">PICMEDRAFT_15388</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018552.1">
        <property type="nucleotide sequence ID" value="XM_019160803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMU3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMU3"/>
    <dbReference type="GeneID" id="30177490"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="174"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="301"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="329"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="501" mass="57394" checksum="30361F1AC5084025" modified="2017-01-18" version="1">MSTYVPLSQLKPPRHAGMKKLDKSKFVINRNVWALFLPEIKYANEFLGRKGKKKFEDSIVLSIPQHPSLIEFSKSMLSGSLVSLISPKNPKLKAILLKEDIKVTDKDKNFANEDDYEPQLLKENVTESTYLLLKKLGSKYKIYNIAFDFDYWSYDDILKAILPEDLIENCPSAFTVAGHLAHLNLKEQYMPYRNIIGEVILNKFPNIRTVVNKTNNITSEFRTFQMELLAGEDNFVVTQKECGCLFTFDFQKVYWNSRLATEHERLINSFDSGEVVCDVMAGVGPFAVPAGKKPCLVFANDLNPESYKYLKENIGTNKVSSFVEASNLDGREFIKQSPKILQNFRKSVSSIKFTDKGMSSKSKAEKAEPSTKKRTMNLDVPLFVSHYVMNLPDSAITFVDSYIGLFSNAYPGLSKEEVKQLTGYKLPVINVHHFEKYKEEEIEGDVEEELKRRMHRKVVEQLNFEISIESLKFHLVRQVAPCKLMYCISFRLPEEVAFAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMX6</accession>
    <name>A0A1E3NMX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="3">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_33876</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017856.1">
        <property type="nucleotide sequence ID" value="XM_019162851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NMX6"/>
    <dbReference type="GeneID" id="30179538"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015980">
        <property type="term" value="P:energy derivation by oxidation of organic compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000007">
        <property type="entry name" value="Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="13">
        <location>
            <begin position="96"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="14">
        <location>
            <begin position="489"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="558" mass="62135" checksum="97305775302DE0F7" modified="2017-01-18" version="1">MANGSQLVRRSAFQGLAGLNSGRLVSTESGAGKSGSGDSGRAEVLKSGVFTWSFRAVVFSGIAGWCYLTWLVYRESNPGSQKPQTEFKENGSRKKNIVILGSGWGAISFLHKLDTTKYNVTIVSPRNYFLFTPLLPSVPSGTINIKSICDSVRTIARQTPGEVTYLEAIATDLDPTNKTVSIEHKSQRFNIGDAFTNDSEPIETTLHYDYLVYAVGATVNTFGIPGIYEHASFLKEANDATAVRQKLFNQIEASRLLPKDSEDRKRLLSFVVCGGGPTGVELAAEIKDYIFQDLSKFIPGIENEMSVTLIEAQHNVLSMFHPRLIEYTKDVFKQQGLNLQTDTMVKKIDDKNVYAAYRHPNGKTEDVVIPYGTLVWAGGNAQRELTKKLASKIVEQQTARRGLLVDEYLRLDGGDQIYALGDCTFTQNPPTAQVAHQQGVFLAEHFNKQSKIEDLKYEIDSTTEESKLNKLTQRLKRLEKKDTPFEYIHQGALAYIGSERAVADLSWGDWSTVALGGTMTFFFWRSAYVTMLLGIRNKIFVVSDWLKVAIFGRDCSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ5</accession>
    <name>A0A1E3NMZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_136200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017876.1">
        <property type="nucleotide sequence ID" value="XM_019160085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ5"/>
    <dbReference type="GeneID" id="30176772"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="113" mass="12733" checksum="17543DCC3611FF3D" modified="2017-01-18" version="1">MSCDHNKPLGVFLRHQTGQFLPRAEYLQHMHIYKSASISLVYTRRTEKQPKAGGRRGGGKVKHEDSSLTELTRARLRHTVRSSTACVCENVCVPVWGIPPLHSMVSQKLPRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN01</accession>
    <name>A0A1E3NN01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018607.1">
        <property type="nucleotide sequence ID" value="XM_019160825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN01"/>
    <dbReference type="STRING" id="763406.A0A1E3NN01"/>
    <dbReference type="GeneID" id="30177512"/>
    <dbReference type="OrthoDB" id="1747771at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="654" mass="72871" checksum="8FA7B35DB6684718" modified="2017-01-18" version="1">MSRKDEDASGTSPRRSSSTSSEDTPIASADTAAKKPDTEGTSFPRTEKPPQSHATAPTDAPQTTTASTTSDTETSPKHSSVENIVLETAQEAPKTESTAESNSEAPLPQHVLLKPSASQKQPNFLRQTLQKYPQSDEDYSSSSSSARKSSPDNASKQKDAQPASSSTKEDTQGSDPSVEAFQLQSKKKRFRRSYNCGPCKTHKIKCDMRIPCGNCEKYDRRDECLKDPPNPPTYQQYLIKQERKRKYLEKRYHLVSSRQDQTAQEPSKGEAADFTAKHTHLSPPQKLQYELTSKPYNDVLQQPSNTISPKPSVNRSESFKYSQPLSNSYGEHDYRVNTNPLIDYKNYYQGAGVPTSTNVHQNTPQIPIFQQQHQQQQQQMQQQQPQQQPPQNPAYAYYPDLAAHYPPQVQMMAPQSYGSHPQIPPQTRFSERAFQPSSTVPQYMEAPYVPLLQPILQPSSYETLPPVPQYPSLPQHVRLPAISEPQHVASVSHVPGLPPPPMMVSNQMHEQYGFRLPIQVPSYPGQPLVAGREQYHAPFPGQEQAPPWASNPSNAYMPNTKTLVPRAYGQSNGGGNATFRLPNMDNIAEEQVSSYRRPPIQQLPNQAVTRSTETNSNLSNFDGSQKLTSLSSVSSLADRFAFTSGAKQQKSTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN03</accession>
    <name>A0A1E3NN03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_57953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018608.1">
        <property type="nucleotide sequence ID" value="XM_019163267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN03"/>
    <dbReference type="STRING" id="763406.A0A1E3NN03"/>
    <dbReference type="GeneID" id="30179954"/>
    <dbReference type="OrthoDB" id="4087488at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="480"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="485"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="594" mass="67121" checksum="E148C5A39AED6698" modified="2017-01-18" version="1">MVSFLNSLSRKSVLKPTDEGPNGDHSPSGSSANPRSSLSLNSRNSIRASVRAFSPNASSPEIKEPNSSNKNSSSSNSNNAGKRSSRFAPSSGTTTASTTSIRSSITEHSNLTSAPSLSQMPLVKRAPVSAVNTSVNHSLSNNHTPVSSNNEDSSIQTKPPKIKDPSISREIENLRTSYRQQNFWKYHIVKLAPHEFYMTTNPDKRHRFIRNAPSYFVKLELPTEANSGDKNVKGEEKKKSTFTSAFKNRSQVDKGFRLVFTQQQVIGINGYDGCYRPFVIEKVPKELGGHYKISCQYNEFQQDWNLHNNVNENNSKSVRNLNFQFTNSNAKPSKNINNPYADADEAKKDKEKEKDKAANIYDAANNLVLADDYTMGDFLHVCVIKEKKKSLFIKSKFDGVKLAKPGSVYFLDTGFFKQRKWFDPIVSVFRPCNRDVKNKLTKSMLVNSKFNMNLSDSKKLFSANKLNVDFSNINLKDGRKHDHDDDVDDDDDDFDYDNDEDEDEEMSENVTDLDNPAGSNYDKFYNAKDGFYSRHPKDDSPNDYKIGWLTIYEKGRYFEKIPNGGNWQMVLGLTFATGFEKMIDKYIKDLAEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN15</accession>
    <name>A0A1E3NN15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NADPH--hemoprotein reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71563</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018609.1">
        <property type="nucleotide sequence ID" value="XM_019163954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN15"/>
    <dbReference type="STRING" id="763406.A0A1E3NN15"/>
    <dbReference type="GeneID" id="30180641"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF127">
        <property type="entry name" value="BIFUNCTIONAL CYTOCHROME P450_NADPH--P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="9">
        <location>
            <begin position="8"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="10">
        <location>
            <begin position="199"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="69804" checksum="9C9BAC876758293C" modified="2017-01-18" version="1">MDGKPNRVLACFSSQSGTSESLAERFCNDLNWKTEDFNLSIVRNTGDFEEPEALKKLEEYEIVIFFISSYGEGEPCDDGVKFFQTLKRWGTKLKQYSLFGCGNTYYDKYQAAAIELKQLMESNGCTLVGEFGQGNEANNSVLDDYDAWSFDYQPVISRLLNVPLKERTEYVPLYRILKPDEMNMQASITERHPYQEMKPFVSDIDLASVRKYGSNYVHFDLELHQEKSRLKYQAGDHVGIYPKNSRGDVDEMLHILGLDEKADPFIAVPFERLGTSGWRGKVFPSYREFFTHNVEINGALSRKLVQDLMSYFIRESATELRAELGMLIASQEVFRQKVLDRRLTLVKFFQKFGITRGTHYDTIPVSFVLEYLGPLKPRLFSISSSECVQPDRVGVMLKLVKDAGSGFDGVCSRTIEEIIGGRDRDGAAAGNAAAADIAGANRLPIYVKRSKFKLPSTLARPMLFVGAGSGMAPFRGFLQEICAQPHKLKQVTQIVVYFGLRSLTDEHYLYRDSFDEFRRVLGDRLVLRLAVSGGASTSPDGIRTKKYVQDLLREDAVAVVDQLVGRNGHVYVCGDAGGLSHGVRKVLVDVLGQARGSLKSGDQYVQYMQSMGRYREDVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNE6</accession>
    <name>A0A1E3NNE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018729.1">
        <property type="nucleotide sequence ID" value="XM_019162083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNE6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNE6"/>
    <dbReference type="GeneID" id="30178770"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="636" mass="74755" checksum="34E92BD888E59B5F" modified="2017-01-18" version="1" fragment="single">MSLQDVRYIDFFPRFYNSLRECKEYAEDSEVRLQLFEIFVNYVLLSTKRHSVSKVIQAFITEENDVHNNNISEQVVDILLEAFQSIKPDTETILLLAKICPDLSASNKEVSDLPFLDRLLATFFRAADEEISESFEDNFVSERLVTFLDILEPKISEPLETYVEILYFSVKNNFDEVSKQILVKLEKLTSYFTSEKYESLLEPDLSFALVTAALKFNKANDATNLIKVLKKKKEIDYIEDEWMTLLQYESFELTEDQPAIQLVKKLNERLSDLDKDYAFGDIDSLNLVFESLCWSNKSFEYIEQFRKDFEDEFGVAMDSKSVATIMNYLCQDKNNSKNVKRASQYFLQYKDQVDWENDYEGFYMLSLFQLTANVWQNPEILWAEKLDVYQNVQRYEYLFNKECVYEMMKAAIKYDAGTFAVKILLDQTPELKKDDPRLEVSKYQKIFDCAYDYLINSTDGELNKRVYKYLADYFAIPYEYYPGFVKIFIDCSDPEMALKVFADMKRQSKESKLPPPGEEFYIYLLKSFAKFQDEEGIFKLHLAIKMDLSINLDIKLLNSLMESYAALEDPFKTRDSAEKNGDYHLINSYVLKTLHNNCLHEGVRNELKQYCQKTFPAEWEELVKTGQLEDNTKDYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNP6</accession>
    <name>A0A1E3NNP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_30808</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018834.1">
        <property type="nucleotide sequence ID" value="XM_019162657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNP6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNP6"/>
    <dbReference type="GeneID" id="30179344"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036436">
        <property type="term" value="C:Isw1a complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036437">
        <property type="term" value="C:Isw1b complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903895">
        <property type="term" value="P:negative regulation of IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046832">
        <property type="term" value="P:negative regulation of RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001178">
        <property type="term" value="P:regulation of transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="156"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="460"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="15">
        <location>
            <begin position="853"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="78"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="747"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1037"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1037"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1068"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1083"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1094" mass="124871" checksum="615208A372E08C8E" modified="2017-01-18" version="1">MTRIQTYPGKKHAQSWTPEEIRKHYFVGDEKKKFDVEATARRFKYLFSLTPLFKHFLQVKASKDDRFAQVMNIVENGTSNGANGAAASAADHRRRRTEKEEDAELLKEEEDETDPAVQDLEQEGEFTDSGYEFSQSPRYVNGSLRPYQIQGLNWLVSLFSNDLSGILADEMGLGKTLQTISFLGYLRYIKDIKGPHIVIVPKSTLENWQREFAKWTPEVRTCVLTGDQEVRNQIIKENVQTCQFDVLISSYEIVIREKSVLKKINWQYIIVDEAHRLKNEDSLLSQIIRMFHSRNRLLITGTPLQNNLHELWALLNFLLPDVFADSTTFDDWFSKDEEAASEQAQEDQDKIVQQLHQVLKPFLLRRIKNDVEKSLLPKQELNVYVGMTPMQRKWYQSILEKDIDAVNGANGKKESKTRLLNIVMQLRKCCNHPYLFEGAEPGPPYTTDEHLVYNSQKMLVLDKLLRRLREQGSRVLIFSQMSRMLDILEDYCVFREWGYCRIDGQTEHSDRIVAIDEYNKPDSEKFVFLLTTRAGGLGINLTSADIVVLFDSDWNPQADLQAMDRAHRIGQKKQVKVFRFVTENAIEEKVLERASQKLRLDQLVIQQGRAGVSGGADKKSSTASNKDELLNMIQHGAAEVLMNGSTGADKQELDDDELERILAKSEEKTGELKSKFSKLGLDDLQNFSSNNESVYEWNGKNFQKKEFKGAGGDGGAGNGFGFNWISLAKRERKGNYSVDGYYREVLQTGNRGNPKDANGEHSANGGGNGGGTEIRPPKHLVLYDHQFYSDELHELHEQEWAYYKRICKIGARADGRGSSKLSDEDVAVEQALIDHARPLLEAEEQRKKDLLAQGYGHWTRRDFFAFVAANAKHGRYDLEAISADLDDKTYEDVVAYSSKFWTSYRDIENYERYLGQIEAGEEKLRKLAMQQEMLRRKVCSYRYPLRDMEFKLPYTATAKREWSGAEDRWLVVQMLRVGVGADDLAEHLFNEIQYGNSVGVELDLWLQSRNPQEIARRCQTLLGAICREQEAKAKLKAAAKAKAQARAKSRAKPRAKTTPKAKARATAKPRGTGSSSRTGKSGSRARPRARPGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNU3</accession>
    <name>A0A1E3NNU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018326.1">
        <property type="nucleotide sequence ID" value="XM_019163888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNU3"/>
    <dbReference type="GeneID" id="30180575"/>
    <dbReference type="OrthoDB" id="5562676at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018786">
        <property type="entry name" value="Mit_KHE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062">
        <property type="entry name" value="K+-H+ EXCHANGE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10173">
        <property type="entry name" value="Mit_KHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="346" mass="39817" checksum="059326B5BB8E3B91" modified="2017-01-18" version="1">MSGWRLRAAGIRGLQIAQIRNLTNASAGADIASIRPTLPGKRIYFYALPLTTKKTFLHCKYNDEIFPGGKKNLEEKIIAKFTQLWNNFAGSEASFNRKVVALINRFLSNIPWLETCLLSIPSQRFITRKLNEDTQYVTHDQILAENIQSDALQKFDFYYPATLTNPELIIQNFKPEFQSQHETHKKGILKDLLLLPLTIPFAIVPLIPNIPGFYLLYRVYCHIKVIASLKYLIILLKDGHFNFDRVDDLAEIYLSTTDNQVRANLMEELTYVSNHRPFQGEAADTDDVADRQEKLLLSEDVARELCKAFGDEESAPKLIFAIEQERRHLKERADALQQQTQHEKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP54</accession>
    <name>A0A1E3NP54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_25228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018426.1">
        <property type="nucleotide sequence ID" value="XM_019162386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP54"/>
    <dbReference type="STRING" id="763406.A0A1E3NP54"/>
    <dbReference type="GeneID" id="30179073"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF1">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15500" checksum="BDF1B908A6250CC6" modified="2017-01-18" version="1" fragment="single">YSSDLEQKLQSLPLVKKYKDDPDYTEYRGWNHLDTTPSGLSNFHGTLLTPGGIAIPPISFHCQKTGDDVVILHVGRRLAGYPFIVHGGMLGMIVDEVFKTNLIREFPNLTFDTVHTKSLELNYSFPTFVNQFLVIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP86</accession>
    <name>A0A1E3NP86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71433</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018466.1">
        <property type="nucleotide sequence ID" value="XM_019163923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP86"/>
    <dbReference type="STRING" id="763406.A0A1E3NP86"/>
    <dbReference type="GeneID" id="30180610"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08286">
        <property type="entry name" value="FDH_like_ADH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42813:SF4">
        <property type="entry name" value="NADP-DEPENDENT ISOPROPANOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42813">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="6">
        <location>
            <begin position="26"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="5">
        <location>
            <begin position="190"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="39127" checksum="4237E59BC3CD0D8B" modified="2017-01-18" version="1">MMKALTYMGPGVISWHSIPKPKILQPTDVIGKVVATTICGSDLHILKGDVPETTAAASKNPEKGLVLGHEAIIEVESVGKDIKKFSPGDICIVSCITSCGSCYYCKKNLQSHCNGNEGTSGWIFGHEIDGTQSEYVRVPFGDTSLFKVPKGVSYDKLLMLCDAMPTSYEIGVLAGGVKQGDSVAIVGLGPVGLSALLSAKTLRPAQIIAIDMDDERLELAKQFGATHTINTGKLTTQALTSEVLQLTSNLSPGREQGVDVAIECVGIPATFELCEDLIASGGSIANVGVHGKSVELKLQNLWIKNCKITTGLVSANTTEELLEKVVDGTLDPSLIITHHFKLDEIEKAYEVFKDAKTTKAMKIVLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPB4</accession>
    <name>A0A1E3NPB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019064.1">
        <property type="nucleotide sequence ID" value="XM_019164059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPB4"/>
    <dbReference type="GeneID" id="30180746"/>
    <dbReference type="OrthoDB" id="3993572at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="243" mass="27704" checksum="6C2953C5B8CDE868" modified="2017-01-18" version="1">MASLEIGNDSDETVCSGTGGKGPENELTRDFLEWSKRPAGSDKKGCRGTGKTPVSLVERYEMLEETILQKLETWLSYMDNFVAVQAVFPSLERRTRDSAVFRPLSFCLRQVAKIYIVMILIYLKRFVLRLQRINRLIGVVKTEAAIIRGNFLMKGSHVTEYHERCLRVLYTEKVKAVVEIVGYANDLVLNLSLVTKRFRLGRALRKLVGFVSWMVNVYRLCRDETQDAKNDAAMSEIEARVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPC3</accession>
    <name>A0A1E3NPC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71931</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019035.1">
        <property type="nucleotide sequence ID" value="XM_019164050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPC3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPC3"/>
    <dbReference type="GeneID" id="30180737"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF11">
        <property type="entry name" value="D-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="47"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>D-serine</name>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="342" mass="37448" checksum="095572C48E10894D" modified="2017-01-18" version="1">MSNVVVLGSGIVGLYTVWSLITEAKVKPSKIVVIGEHLPGDLSINYTSPWAGGNFSCISPNDADTLSFDKFTYTNVAKIQKVLGGPSCGLDMKPSTEFWDEYPGDAKINSLKGYLKDFKVVPSSELPAGTAFGITFTTWNFNCPLFLANFRTYLEKTGVSFRRQKLTHISQAYLPHTVAVFNCTGIGAFSLSGVKDSKVYPTRGQVLVVKAPHIQENRMRWGADYATYIIPRPNSGGKLILGGFLQKDNWTGDTFEEETKDIIKRTTELMPKILDQPLEILAVAAGLRPSRHGGVRIEAEKVEEKLLIHNYGASGYGYQAGLGMGHKAVQLFQTTGKVSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPF9</accession>
    <name>A0A1E3NPF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_71650</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018711.1">
        <property type="nucleotide sequence ID" value="XM_019163975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPF9"/>
    <dbReference type="GeneID" id="30180662"/>
    <dbReference type="OrthoDB" id="416496at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000371">
        <property type="entry name" value="Methyltransferase OMS1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF83">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="12">
        <location>
            <begin position="224"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ47598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="433" mass="49969" checksum="99B8473C0993FE15" modified="2017-01-18" version="1">MRLVQSRLYAGGTKFPPRPPKFQHKTKKELTAEEQKQVEEERKYHEAVNSGSAVKKWGAILGSSGFNKRATKYYIGLYVVFLCYGIYYFRKLFGRHLEEEDLKKLRDSGAELSEYQKLRIRDLAGDLLRTKDTQKLKAYYVLKEAYDAQPEEERAKAGPFDPAPEDIRDVMDVSFEQCIEPPKDLSGFYDALAAEYDSDVGTEEMMSFMGGKRKWLMRHCKGDVLEVASGTGRNIEYLDPTVVRSYTFLDASRKMMDVCYDKFHERWPNFERAKFVVGKAEELVKLSAAGPSGRTFRYDTIVETFGLCSHEDPVQALKNMRALLKPGGRIVLLEHGRGEYAFINKILDRRAHKHSEKWGCRWNLDIGDLVDESGLEITEEKRAHLGTTWMLVCKRPEDVLDIDEMSALEKYFFPRRTNLDSSNIVNRDDSRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPG1</accession>
    <name>A0A1E3NPG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphotyrosine protein phosphatase I domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_58327</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019107.1">
        <property type="nucleotide sequence ID" value="XM_019163292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPG1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPG1"/>
    <dbReference type="GeneID" id="30179979"/>
    <dbReference type="OrthoDB" id="3388at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16343">
        <property type="entry name" value="LMWPTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000105">
        <property type="entry name" value="Low molecular weight phosphotyrosine protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050438">
        <property type="entry name" value="LMW_PTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002115">
        <property type="entry name" value="Tyr_Pase_low_mol_wt_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717:SF7">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PHOSPHOTYROSINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00720">
        <property type="entry name" value="MAMMALPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="9">
        <location>
            <begin position="7"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR617867-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="164" mass="18451" checksum="0831962D213E21C6" modified="2017-01-18" version="1">MSSDTSIKVAFVCLGNICRSPMAEAVFRKIVNENKLADTVTYIDSFGTAGYHVGDRSDSRTIKTCKNHDVPINHRGQQIKSKHFTEFDYILCMDDSNLYNLKRLKPKNSTAVVGMFGEWKDSDSFDTVIDDPYYGGTDGFETCYQQCVSFSKAFLKKELGVNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPJ3</accession>
    <name>A0A1E3NPJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71984</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 9">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019098.1">
        <property type="nucleotide sequence ID" value="XM_019164064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPJ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPJ3"/>
    <dbReference type="GeneID" id="30180751"/>
    <dbReference type="OrthoDB" id="6718861at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004354">
        <property type="term" value="F:glutamate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05313">
        <property type="entry name" value="NAD_bind_2_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.285.10:FF:000001">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.285.10:FF:000003">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000002">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000030">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.285.10">
        <property type="entry name" value="Glutamate Dehydrogenase, chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050724">
        <property type="entry name" value="Glu_Leu_Phe_Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006929">
        <property type="entry name" value="PRK09414.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43571">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43571:SF1">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="189"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="8">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="461" mass="49951" checksum="2A430C1522168226" modified="2017-01-18" version="1">MVQPEEPEFQQAYNELLSTLKESTLFAKHPEYEKVIPVVSIPERIIQFRVTWEDDSGAMQVNRGYRVQFNSALGPYKGGLRLHPSVNLSILKFLGYEQIFKNALTGLPMGGGKGGSDFNPKGKSDNEIRRFCVAFMRQLARYIGQFTDVPAGDIGTGGREIGFMFGAYKQMQNQWEGVLTGKGLSWGGSLIRPEATGYGLVYYVEKMIEKATNGKETFAGKRVAISGSGNVAQYAALKAIELGSTVVSLSDSTGTIINQKGISPEQIYAIQAAKLQFKSLKEIIATDKSFTTQSTKCEYLPGERPWVHVGAVDVALPSATQNEVSGEEAKALVAAGCKFIAEGSNMGSTQEAIDVFEAERKSASALGASIWYAPGKAANCGGVAVSGLEMAQNSQRESWTAEEVDQKLKDIMVNCFENCYETSVAYSAETNKGSLPSLVMGANVAGFIKVADAMKDQGDWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPL8</accession>
    <name>A0A1E3NPL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_114314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018606.1">
        <property type="nucleotide sequence ID" value="XM_019159793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPL8"/>
    <dbReference type="GeneID" id="30176480"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="127" mass="13448" checksum="8EF16757101D0DF0" modified="2017-01-18" version="1">MYVRVLQNEHGRPARELYFARILPVPALTPSALRSDAPPGAGTTVPVTHRADPLLHTPTGVPYPDRACFGVRWQAPALGLAGTASCSPRPVPGSLPLPPTAYCRIGDTGTHADRPIQVNPCRIGPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPP2</accession>
    <name>A0A1E3NPP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019204.1">
        <property type="nucleotide sequence ID" value="XM_019164090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPP2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPP2"/>
    <dbReference type="GeneID" id="30180777"/>
    <dbReference type="OrthoDB" id="10068368at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14232">
        <property type="entry name" value="GAT_LSB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16980">
        <property type="entry name" value="VHS_Lsb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044103">
        <property type="entry name" value="GAT_LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045007">
        <property type="entry name" value="LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789:SF1">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="2">
        <location>
            <begin position="41"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="457" mass="51979" checksum="397E8EC571CD9F47" modified="2017-01-18" version="1">MGFLNRHQHTAVTSTINDCCEKNDPSGDDFYIETQLSVLLDAIKFQKDFAEPGPVEAARALRKKMKYGNTKEQYNALRVLDLLITNGGREITALCNDDKLLDRLQFYLMDGSTSSFSSSAASPPNKKISNFTRSLMAGWYDKYRDDDRLTNITLLYERCMQGRKAKQRAAATSSYDRQVPDFMNDEADMDYTPFDDDGEDYSNGEEQSRYSYADEPSRYSTNSSSQSRPKTNAELDKKFKIPRINYDKEGPKIMQLIGQANIMATNLLNILNSLDKNELSIHSIRANDAFDECRAIRRKVLRYLQLVNREELLGPLLKCNDDLVISLKKYEEHSVPAEARVTTNSDSDDYDSLQDYESDHEPVNLPLGTARRAPISDDDYDTSDSDDDDRYVRERAPPAGTRYEVKERRAPPPVPAKKSVLQSPKPVKQTSIMKEKVSKSDYDPFSDDNEVAPVSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPT7</accession>
    <name>A0A1E3NPT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15888</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Preautophagosomal structure membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATG5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019159.1">
        <property type="nucleotide sequence ID" value="XM_019161006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPT7"/>
    <dbReference type="STRING" id="763406.A0A1E3NPT7"/>
    <dbReference type="GeneID" id="30177693"/>
    <dbReference type="OrthoDB" id="272162at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="5 7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="10">
        <location>
            <begin position="11"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="9">
        <location>
            <begin position="138"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="8">
        <location>
            <begin position="198"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="290" mass="33731" checksum="BC6F968BD38B8B43" modified="2017-01-18" version="1">MPGENSILSKIWSSQICCKVNYKTNTTNSRQTFYLLVHRNSYLYRLLAEIFDFFNIPHSKISQAWFEDARTGQIVDWNLPLDVSYELYTDMPDRHGYFDIDLRLKFISSSQSSQSIKAFQQQLASTASTSPASGVVKLLEKHWRNIIKESCYILNGSSNIIMSMSLENSKEFWNAVLLHDYKSYEKHFRKLLPSSPMNVPLKLYIVENKLLKFVNQPNLNRMFPRTPLHEISLQNLVDSVLTLKSHREHPNRKPLQFIAHGVQLPPESPLNELYTAAKYFDSFLHIVIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPY6</accession>
    <name>A0A1E3NPY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(32) synthase</fullName>
            <ecNumber evidence="3">5.4.99.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71810</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(32) in tRNA = pseudouridine(32) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10107"/>
            <dbReference type="Rhea" id="RHEA-COMP:10108"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.28"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.4.99.28" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018891.1">
        <property type="nucleotide sequence ID" value="XM_019164015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPY6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPY6"/>
    <dbReference type="GeneID" id="30180702"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160151">
        <property type="term" value="F:tRNA pseudouridine(32) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000061">
        <property type="entry name" value="DRAP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000017">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18785">
        <property type="entry name" value="Inv-AAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="6">
        <location>
            <begin position="421"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00036184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="577" mass="65334" checksum="FF71DC0929D83792" modified="2017-01-18" version="1">MTEQWAETGMNESFKPMKKRGAVPLVDENGFKVRKVQDVAKHTPASGSDKTLKEEDENTRYIIDGKLRRTTPYFFTYMTYCKLRWRDRTLLDIFSNEFRLYPESYYIKALETGQVTLNGEKADKDAIIRNGDLICHRIHRHEPPVSSRPVKIVYQDEKIVAIDKPSGVPVHPTGRFRHNTVTYILKNEHGLVVHPCNRLDRLTSGLMFLGKTAKAAENMVDQIRNREVTKQYIAKVVGEFPIEETVVDKPVYTYDPRVSLNIVDEQKGKEAKTVFKRLSYDGENSIVLCKPYTGRTHQIRVHLQYLGHPIINDPIYSSPDIWGKNLGKDGEFDMAKVVGILEQVGKTLPTSSWLHRNSERAELGEMLNGEKCEVCGQDLYTDPDPNDLELYLHAYKYESKIDAEDENGWSYNTELPDWAQEHAKKYMGLAMEEARKSEPTPTAFCVGAVLVNGGSVLSTGYSRELPGNTHAEQCALEKYFTEHNTHKVPAGTELYTTMEPCSLRLSGNETCLNRVLKQDGNISSVFVGVMEPSTFVKNNVSYDKLTSAGINYIKVDGYDEEAVQIAARGHQLVNVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ09</accession>
    <name>A0A1E3NQ09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CWH43-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71682</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018746.1">
        <property type="nucleotide sequence ID" value="XM_019163979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ09"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ09"/>
    <dbReference type="GeneID" id="30180666"/>
    <dbReference type="OrthoDB" id="10032492at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF2">
        <property type="entry name" value="EG:22E5.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="7">
        <location>
            <begin position="16"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="339" mass="38442" checksum="0AA8BD3C06A18523" modified="2017-01-18" version="1">MKIGALKLPNVKPQHAWILPLISLAGWWAMLVALMICWFAKGRPILDTDHTEMPHFIVYLSNIAATDLQPIFICGSAVMGIFFVWAVIEDYYLRTPEKNYFPVTFHWFVTGLHALAIALCIIGCLCILMTSCLKDNWQYSRPHSVFVILFVIFVFAYICAHSLAFLLHYRHYKIRYFMLSAVAKIVWTCIAIVLVVCYGAFMGKANAEGLHSKYWGYSAIMEWTLVFFYGLLMFIITSDLRRSNYGDYVEYYVNREADLRHKEFDESSNMDYGETTFDTSGMNAAANVDATTNASATGNNPGLEDLTAVSQHETWDNATPFDRPGARPGVAPTSSRYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ76</accession>
    <name>A0A1E3NQ76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_120385</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018981.1">
        <property type="nucleotide sequence ID" value="XM_019159873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ76"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ76"/>
    <dbReference type="GeneID" id="30176560"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="9">
        <location>
            <begin position="42"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="10">
        <location>
            <begin position="352"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="713" mass="78093" checksum="3D45C87A5CDA2938" modified="2017-01-18" version="1">MKKSAVPAQTSGDIFTAISTAEPSTSLYPRLASTVAVPSNATKSGAVQTNNFWGNISLGDQNSVVFTHPYVLWKSTTSDFNGLAISHQTDSQKVWGDKNDHGASSYFYSAAGVMSLVLGSKDFDNGCSISLQNMKRMSIDVTLSSPNAGSLTASVLQGMGMVTAVYNDLIPEIHSQVGISSVTGGTAPKTGTDKYKIVLKDNSTWYMYVTFTDNQTCKFALKDSGHIIGSNSINNCWIQVCCGDFSDYDRAAGCYPTACTLSASTSGSTGTYALNYTTAGSGTTPLMYALPHHVASFTSKTSNTKSNASLDDTVHGTMVGYLTNVFEMSENLPTSLGMDPWSKISGFSTPNYSDDELNAIRAAASSDIQNDVANLSNLDTMYYSGKILDKFAYVLYVLYYVLKDTDSTKALLDKMKTAMNRFTNNQQINPLCYETNWGGICSTAGMAKGDTGADFGNAFYNDHHFHYGYHIHAAAITAKIDQLVGDGSWLTNNKDWVNNLVRDVANPNESDTYFPVFRNFDFFIGHSFAHGITIYSDGKDEESSSEDYNFAYAMKVWAQVIGDSNMETRANLILAIESRAIGTYMLYQSNNNAMPSNYTGNYVSGIMFENKIDHTTYFGTNEEYIHGIHMLPITPVSSLIRSPAFVKEEWENFLSSRIDGINSGWKGVLMMNVALYDPGTAYKFFSQPNFNYNYLDDGMTLTWSLAYCSGVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQB7</accession>
    <name>A0A1E3NQB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_184955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019417.1">
        <property type="nucleotide sequence ID" value="XM_019162027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQB7"/>
    <dbReference type="GeneID" id="30178714"/>
    <dbReference type="OrthoDB" id="4069986at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="49" mass="5832" checksum="D64237DA05D51DB2" modified="2017-01-18" version="1" fragment="single">MPMHQEELTDIYIHLKDILKTLSQTPVDREFYADGLINDMIKIKEILEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQB8</accession>
    <name>A0A1E3NQB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15642</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018846.1">
        <property type="nucleotide sequence ID" value="XM_019160904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQB8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQB8"/>
    <dbReference type="GeneID" id="30177591"/>
    <dbReference type="OrthoDB" id="2013116at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053050">
        <property type="entry name" value="ALS_regulatory_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242:SF2">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="76"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="298" mass="32593" checksum="A70084F2F3E05AB1" modified="2017-01-18" version="1">MLSKSVGLRQCNPLGSLSLFRNSSSTAAIAYKNLHRNKKRPQLPTFENPTLSADSAVSSIIYETPIVSKTPKTQHVLNCLVQNEPGVLSLVSGTLAARGFNIDSLVVCNTEVKDLSRMTIVLGGQDAVIEQARKQIEDLVPVYAVLDYSHSEIIKRELLLARISLLGSEYFQELVAHHNDADVKVSEKLRTEVYHPSNLPPSQALRQKYEHLDAISKLTREFGGKVVDVSDRNCIVELSAKPTRISAFIALIQPFGILELARSGMMALPRTPIEGVEDGTDPTKDAADVVDVSQLPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQH8</accession>
    <name>A0A1E3NQH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_69987</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the laat-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019454.1">
        <property type="nucleotide sequence ID" value="XM_019163524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQH8"/>
    <dbReference type="GeneID" id="30180211"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000009">
        <property type="entry name" value="PQ loop repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF34">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="253" mass="28248" checksum="2F87451D2A3E53EA" modified="2017-01-18" version="1">MENSSRLLSLVSTLCWIVALLPQQWHNYCRKSVDGLSPYLLILWLLGDSFNLVGCVLTEQLPFQTYLSAYFVLNDVILDFQYYYYRGRRPDAGSSVELLADDGYTSTVSSSSVASHVPVLTKTQVVTTATALLSASPVAAVPLQASALSAAFDGEAVGMVFAWLCTLLYCSSRLPQLYHNYLRKSVEGVSPLLFTFAVLANLTYALSILLADPPHGMTYRGFIWNETPYLLGSLGTVWFDGLYFWQREIYREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQI4</accession>
    <name>A0A1E3NQI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flavoprotein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15832</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019081.1">
        <property type="nucleotide sequence ID" value="XM_019160987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQI4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQI4"/>
    <dbReference type="GeneID" id="30177674"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="403"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="172"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="285"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="637"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="185"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="637"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="667"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="718"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="747" mass="82284" checksum="B3E1FAEC3F49148E" modified="2017-01-18" version="1">MFNTDVSFNKGIVNSMNKEEMRSPSPLSDQRHQSSLVREDQDERKTLEIMRPKFRKSSSSNSANSLKLAQLPHLETSIGYAHDVAPTEKKHIVNVNESLLETVKGNKKIPTSSTDSLTSRANSNNNSVGNANQSVVTSQPKTATVPASILASSSYPTPITPQLKTSTSTISFQVPNQKHRGSISARPSTTKVPLLDSPQTGIPSSRQPSVSSTTQAPLSRQASQSASFPSSTNTNIPNVNTSTPHNALQKLGSSSIIGANISRREDVRSPVQFDLLNGVSVASHITPNNKVSTGTNSNTTSSNHEIHTSPRIAEENNNLPTTRLLSAANSKLRNSSLPGENIYESKSGMSSTTSINGGNPNSNLSTPSSITKKKTLKTVVTPPVGPLVPFTQYLCKEDDDKVHILIGATGSVATIKIPMIIDKLFKIYGTNKVSIQLVVTTAAEHFLRGMKISSEVKIWREQEEWSAPVNKIKPGDPILHVELRKWADIFLIAPLSANTLAKIANGLADNLLTSIVRIWNPAVPILVAPAMNTFMYMHPITKIHLNILAEDFKFIEVLKPVEKVLVCGDIGMGGMREWSEVVDITVKKLKEIRRRKLELEKAKLDLIENGDNPEVNELQEKVASIKIEAVKDLDTNGKECKAKGKGREEVEGKEKVEEKEEKKEEKEEKEEEEEEEEDDEDDEDDDDDDDDEDDEDYDDEEEEDDEDDEDDEEEGEGEREGEVGDQRDLQEVGKIDRAGDEKIPVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQN1</accession>
    <name>A0A1E3NQN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_27922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018966.1">
        <property type="nucleotide sequence ID" value="XM_019162523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NQN1"/>
    <dbReference type="GeneID" id="30179210"/>
    <dbReference type="OrthoDB" id="5586401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039870">
        <property type="entry name" value="Coa4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13639">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13639:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHCH" evidence="2">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="61" mass="7377" checksum="E822181DEB42A6CC" modified="2017-01-18" version="1" fragment="single">DDDQPDEWDSRIISTGCAEENLRLQLCHYDTNDWRQCTKEMEAFRRCWADHSNNERTRTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQR8</accession>
    <name>A0A1E3NQR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 10">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15894</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019161.1">
        <property type="nucleotide sequence ID" value="XM_019161008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQR8"/>
    <dbReference type="GeneID" id="30177695"/>
    <dbReference type="OrthoDB" id="422086at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="225" mass="25030" checksum="324CA05FF3214DEE" modified="2017-01-18" version="1">MDNPLPQVSATAYALGIVSGAALALALGCPLFASFVYLPLYVCSVATFHFLEYFCTASYQPARVDVSSFVLNDTALYHVAHATALSECLVELWLVPGFKKNPGLRPVKLVGLLLTVAGQCTRSLAMKTAGQNFSHVIQRKKEQGHVLVTHGIYAYLRHPSYVGFFYWALGTQLLLLNPLSLCLFAVLLYRFFSSRIRYEEGCLTHFFGKDYVAYKRTSRIGIPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQY3</accession>
    <name>A0A1E3NQY3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_32696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019106.1">
        <property type="nucleotide sequence ID" value="XM_019162784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQY3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQY3"/>
    <dbReference type="GeneID" id="30179471"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="246" mass="28531" checksum="F9ABD259719FB6C5" modified="2017-01-18" version="1">MALRETYTPRGEQRKLVENLLGKGASLKGDSIDVSILDPPRMCKSFIVGKCPYDMLDNTKENMGKCPKWHIEKHKLIYETAKESGIKMPRQNYELDYMNDLQHFVGDCDQRVRIAEERLDYSEADKQLLNDLARTVENLEKTISITSEELNVVQEEKRDMQRSIELNEELQKFILEKETVSERYSATLERLNTVGQQKLQVCEVCGAYMSKVDNDRRLADHFLGKIHIAYAAMRNTLAELREKHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRD3</accession>
    <name>A0A1E3NRD3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_24429</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019236.1">
        <property type="nucleotide sequence ID" value="XM_019162352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRD3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRD3"/>
    <dbReference type="GeneID" id="30179039"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="207" mass="23987" checksum="9C9009CC9794A45B" modified="2017-01-18" version="1" fragment="single">IIGKGAGGSVYPLYDKAHNHLYAMKKLRLQMSRENWVSYETKLKNEFRIANKLHHQNLIRTYDLLQDNDLFIIVMDYAPFDFFTIVMAGVLTKHEIYCYFKQMCIGVSYMHSIGLAHRDLKLDNCVVDANGILKLVDFGSSVIFDMKRYNQEGESREVEKASGIMGSDPYLAPEVVTYPFYDPSLADVWSLAIIYCCMILRRFPWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRH5</accession>
    <name>A0A1E3NRH5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70290</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019782.1">
        <property type="nucleotide sequence ID" value="XM_019163603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRH5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRH5"/>
    <dbReference type="GeneID" id="30180290"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="309" mass="35774" checksum="555F14C686ECA9A5" modified="2017-01-18" version="1">MGLLKVSYIVTLTTVLWVVLTTLFPNIFPGYYDHGDMQNKDGYLYSAYYNSLNTPSILKNNLTDSHILLFTAHPDDESMFFTPTITELTKENYNNTFHLICLSNGGYDGLGSIRERELRKAARVLSIDSVKVLDYVDDINVYWNISDIVKSITDEINNVKREYEFEENKSLILLTFDQDGVSSHPNHKSVYQAVKTYNKLTNTRAYSLKSWNILVKYSGMLFTNIEVAMKWLAKCDDIKSFLSRINISLLDHIIADISKESIAIYSDLNSLFLNFSTMTWVHFSQIVWFRWIWIFSSKYMNSNELVPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRK5</accession>
    <name>A0A1E3NRK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_29981</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019812.1">
        <property type="nucleotide sequence ID" value="XM_019162615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRK5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRK5"/>
    <dbReference type="GeneID" id="30179302"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF36">
        <property type="entry name" value="PROLINE-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="76"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="582" mass="63925" checksum="D6C80C5491E1F014" modified="2017-01-18" version="1">MYKYFKKDIKSDVSIQTYPDDSAVETGETGHSSAFKQYESPDEKLGQLEYTNSSSSKIDPEDNGVGELKRGLNSRHVQFISLAGAIGTGLFVGSGAALSIAGPAGLLIGYLVLCFFVWSIMNQLSEMVTYLPIPGKSTPYALCDRYTGNKSLAVAAGLNLFYAQALLAPAEISAAAFVIKYWTDLNVAIWISIFWGTIVALNFCAVKVFGEIEFWIASIKIITLTGLIIVGIVIFFGGAPDSHGVLGFHYWKKPGAFVEHLTTGNTGKFLACWSSIIKSAFAFILSPELITICAAEAEAPRINLPKAADRFIYRLFFFYICGVLVIGCIVGSNDSRLMNAINEGSSGAAASPFVIGIQLAGIKVLNHIINAAILSSAYSAGNSFVYASSRTLFSMASRGDIPRVFGRVNRWGVPYNCVACSAAVCLLAYLNVSDSSSQAFTWFSNISTVSGFIEWIIVSITYLRFRKALQYHNMDDRVTYRPPLQIFGAYACIFFFSLVTLTNGYAVFFDFNGSDFVAAYITIPIVLFIYALHAVWTRNWKWMAPPEEIDVTTGLAEVEEETRNYVPAVPKNILQRIWFWVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRW9</accession>
    <name>A0A1E3NRW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_27914</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019551.1">
        <property type="nucleotide sequence ID" value="XM_019162522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRW9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRW9"/>
    <dbReference type="GeneID" id="30179209"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF3">
        <property type="entry name" value="ABR032WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="236" mass="27277" checksum="CA747808F415673A" modified="2017-01-18" version="1" fragment="single">PERAPASPAAGGSTSGKKLRKINKPPKSYAKSIWLVGHIMTLGFGVFYSLYYFQRKSQHRLVPWVCYKLTLMGVWTAYVTSIQSQYNIKSLPHYTTLVATENFQYLLLSVMWFFNRNSFFKILPFMIVSLLQIATSCKLTAILKLETQLSKVVLYDELFLFVLLLVDTLLLRGTSGYGLVTYCMFMWLRILQSENTRFFLYDNLIKLDSQISKIKNPKVQQGWLGVKKFLSYKQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRX9</accession>
    <name>A0A1E3NRX9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_29360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019561.1">
        <property type="nucleotide sequence ID" value="XM_019162588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRX9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRX9"/>
    <dbReference type="GeneID" id="30179275"/>
    <dbReference type="OrthoDB" id="1690618at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006452">
        <property type="term" value="P:translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23953">
        <property type="entry name" value="zuotin_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.840">
        <property type="entry name" value="Ribosome-associated complex head domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032003">
        <property type="entry name" value="RAC_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042569">
        <property type="entry name" value="RAC_head_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058871">
        <property type="entry name" value="Zuotin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16717">
        <property type="entry name" value="RAC_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26185">
        <property type="entry name" value="Zuotin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="83"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="420" mass="47194" checksum="CACCF253CE02B8DD" modified="2017-01-18" version="1">MSFSAAASYSAAVKRPVEPVGPYFLAHATRTLKGHSWSEYEQIEASKNVVNVEERDADEDLFDAEISDPELLSHDPRDWKSCDLYAALGLSKLRVNATPDQIVKAHRKAVLQHHPDKKSSAGGNLDQDGFFKIIQKAFETLLDPVKRRQFDSVDESAEVAPPANKCTTEEEFYAAWGPVFVSESRFSNKQPAPPLGDSNSTKEEVESFYKFWNNLDSWRSFEWLDEDVPDDTSNRDHKRYIEKKNNNSRKKKKNDDNKRLSDLVKRAQAEDPRIKKFKDEEKAEKARKKWEREAGSRKAEEEAKAKAEAEAKAKAEAEALAKAEKENSKKSKEAAKSAKKKNKRTIRGAAKDNEYFDDAGKQSAIDADIDLLIESFDDLLLTEVASKLKDAKADAAKQILIDAGAKQLAAGKVSSLSYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRZ2</accession>
    <name>A0A1E3NRZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_29031</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR59 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019964.1">
        <property type="nucleotide sequence ID" value="XM_019162571.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRZ2"/>
    <dbReference type="GeneID" id="30179258"/>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110009">
        <property type="term" value="P:formin-nucleated actin cable organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21294">
        <property type="entry name" value="CH_FIMB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21297">
        <property type="entry name" value="CH_FIMB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21300">
        <property type="entry name" value="CH_FIMB_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21303">
        <property type="entry name" value="CH_FIMB_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000016">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000027">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="168"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="12">
        <location>
            <begin position="413"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="1442"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="1590"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="1714"/>
            <end position="1824"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="1837"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="546"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="702"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1399"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1250"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="555"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1400"/>
            <end position="1419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1945" mass="220789" checksum="2FB9BFB94BB9A5E0" modified="2017-01-18" version="1">MSIAPCGRDAVLAGRKGLLVIDLDDPFAPPRWLHHETSWEVADVQWSPHSSKPSWIVSTSNQKAMVWNLARPSNDAIEYVLHSHIRAITDIHFHPQNPEMLATCSVDSFVLAWDLRCPKEPVYQWSDWRSAASQVKWNYKDPNIIASSHDNNILIWDVRKGAIPMKSIEAHDAKINGLDWSRENKYELISSSNDMSVKFWSFDKEYDKPVYTIRTDFPVSKSRHVPFGDHICGIMPLRGGNDSVYIVDYKNKVGESSLAPCYIFKGHTEPVRDFVWRSRHTPNTSVNDSEYQLVTWSSDRDLRLWPMNDDMYKNFNFKRNNQLPPGQKLSEFEYRSYRPEPNVSSENKLVIRHKSRRYKGSSYGESFKNNSEFNHLTWISGIKIGQSAFQHIDNDPNSATFGDNSNQPLNLGEEVSNVGHKFPKLRFERISVSTGVLVISLNGPWSLQDKDDLIFIRVEFNFPKGYPSSKAVPKFKVEETHELSSEKKQEILKNLQEIATKFCAHERFCLEPCLRFLLGEKVDMDFEVEETLESYDLNYSEDTINKNLMSVSPGGDDEDEDDEDDDIDDISDNNNLKNNFKKAPFDSTPVSKGCGAIWTPSGHLVCFFISKETNDDQHFIKFGQQGFSLVRNLKRKHVENGLQLGKQVFIDGALSNKDEPFSDSSSSDDSLSNDFDVFQYNRLYRTKVPDLLRNANLNTRQYSSNAKSNPMSAPTERSQVTNNSKTQHSRNIVKIYDFRHLIPSKMELAYEYRVLGDSPHVLAQYNAEVAEKYGYTVISNCWKILSILLVKDVMIDADIATNILQKIGESTNNFELLRNYRFYWGFHPFGGIWLVKQLFSYFKKVNDIQMLAMLSCVLFENDVIKDHVHVPINSPYTRMETHTDTKKSILRNGSGAQRTGSCRVPSISTLSSYEYDNRSFKSSSPAGDIVGGNNVLHNRRGIKHQVLSNGGGGGTGNGSGGDFDHYMQYDSISNTPQLSRSLSTASLNVFNQQFGKSLSMAKTPLAEEKMFVRYDDMPIVNIQMQNFAELDLYENEYCVNIEGFVNPEQLKVFRAEYAAILFCWGLPASRLKILKFNYNYNQNYNCNAVSIANTDISFLSKTDSMILNSDNKGDMNEYDYKTFSTRHPLRFSKVKAFEQAELTTKMYFSNDSNCEIIVQIPEMSRRETSAMLGHDTVETKIAENGSNRLKQQFGGGFMQNPVEQRLFLMKNALKPLSKEEIVCSKLNQSKLPDIFQNTGQFLKEMDPQEIEFWELMNEEFENDLQDLQVEYKGDQQRCRRLVDEATVQTFFERVKSNRRLGRRRHPGVERSLGALICQRKKYPILDQSELFSLIEDFRNIDIDENGWADKKDVIESVSKSGKGSYDEVREALKNVSVDASGHVELDDFVELNAKLKEAKQSGGSSGPSSAQMAGSQSGAETFVRNKAKVVLGSGRTMHTIDDEERVEFTRHINSVLAGDALVGARVPFDLDTFQLFDECTDGLVLSALINDSVPDTIDTRVLNLPKGGKPLNNFKLLENANIVLNSAKAIGCIVVNVHSEDITAGKEHLILGLIWQIIRRGLLSKIDIKLHPELYRLLEDDETLEQFLRLPPEKILLRWFNYHLKAANWHRKVNNFSQDVQDGENYTVLLNQLAPELCSRAPLQTADLLQRAEQVLSNADKLQCRKYLTPKSLVAGNPKLNLAFVAHLFNTHPGLEPVEDEEKPEIEDFDAEGEREARVFTLWLNSLDVDPPVVSLFEDLKDGQILLEAYDKVMPGSVDWKHINKRPTNGNDMSRFKALENTNYCVAIGKANHFSLVGIEGSDITDGTKILTLGLVWQLMRRNINNTLSSLSVNGKEVSDQDILKWANSMVAKGGKSSSIRSFKDPSLSTGQFLLDVLHGMKPGYVDYDLVMSGNTEEERYANAKLAISIARKLGALIWLVPEDINEVRSRLILTFVGSLMSLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS50</accession>
    <name>A0A1E3NS50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger C2H2 LYAR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_70469</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the UPF0743 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019987.1">
        <property type="nucleotide sequence ID" value="XM_019163647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS50"/>
    <dbReference type="STRING" id="763406.A0A1E3NS50"/>
    <dbReference type="GeneID" id="30180334"/>
    <dbReference type="OrthoDB" id="21474at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.490:FF:000001">
        <property type="entry name" value="cell growth-regulating nucleolar protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039999">
        <property type="entry name" value="LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014898">
        <property type="entry name" value="Znf_C2H2_LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100:SF10">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08790">
        <property type="entry name" value="zf-LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51804">
        <property type="entry name" value="ZF_C2HC_LYAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger C2H2 LYAR-type" evidence="10">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="62"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="172" mass="19692" checksum="78325DA3CC60DB98" modified="2017-01-18" version="1">MVSFSCEVCNDTVLKKKLQQHQRSCHGAYFTCIDCSTTFYGNDHQKHTSCISEAEKYEKALYKGKKGKNQQQQQKQQKLPQQNQATKQQQHAVEAKKEIKKDSEKPKKAEVPKKESLSSSSNISKYVDSKATTLYKIFKSAKKDDKKFADKTEFLKNVKVTQNEDGSFNLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS54</accession>
    <name>A0A1E3NS54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Integral membrane bound transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70502</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020022.1">
        <property type="nucleotide sequence ID" value="XM_019163659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS54"/>
    <dbReference type="STRING" id="763406.A0A1E3NS54"/>
    <dbReference type="GeneID" id="30180346"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF1">
        <property type="entry name" value="DUF2421 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="663"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="694"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="718"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="741"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="765"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="802"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="7">
        <location>
            <begin position="685"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1054" mass="118761" checksum="B83785AB3DB3ABD0" modified="2017-01-18" version="1">MNEPSPEGDESDDYADFTVNSINNLNAGNNDETAGTDKDSTPFSRYLNRAKSTSSFIIHPQRKSSIIVAATGEKIQRAYSTWGQLSTSASSVKPVTDATLLISPEEESVRHERFSTLSSVSRRYSSFTKYMRKLATKPILKAAIAYFLASLTVYSPTISNLLGSSDSKHMVCTVVVYFHASRSVGSMIQALMFVSFSLLFGIGVSVTTLSSISLYLDDTSSSMAHLREINMQSPLIISLTLTVCSISLGLISFCKHRVRKQTFNTACSLAAILMISCLIKEYSNILDSDPTNIVIPWSKIISSISCVICGCCISVFVCFTIWKNWAETELIAANNSVKTSIGDTLVLLCDVFTSSDCTSSDIESDREKISKSFTDLKGKRGKLDSSLEETMYECLIYGREKEFTLYKDLVESEKKLIAYLGGLRRAVEFKWEMIEYYETMRGGPIDDPFKSDASMGNLDVLNENDEDTENSIDPLSADTDLVKNPEELIELFLYHLAPSTKSFVFTMNQILRGSLYDSKINVDPLVKQYSKSLALAHELFETHQKRAIDSVYKQDIFTKNDGLDEKVNQEEVAATCANFSFSITEFSLELAKLLNIMTSLLDSSDDIERSYNFLKFWHRGKKSLIDTERNQLVNADLDQLENETVGYKFWKITKFTRGVDFQFGFRVGLGALILGSLAFWNATRHIFKEWRGEWILVTYCIIMNKSLGGTTMTLKWRFLGTFVGAVTAFTVWSLFYPNVILMAILGFFVSLPCFDIILNWKANNAFGRFILLTYNLTVLYSYTMSLNDISDGDDWEGGGNPIIWEIAFHRYIGVSLGVVWAIVITLTLFPVTARSRIKRGLSILWLRMGIIWKRGALYKRRDEYGQDTLDGLRGLKDCHSIMNELRILLKQAPMEIRLKGPFPIEIYTRLMDGTEGILDAYENINSIVDIDPVLNPVESLVLENVREEMSELQNRVFLIFYMLASALKLGIPLASDPATTEHTMVKLLSKLGDVRRTVEARREIGKYTGLKNTDFVLLYSYCLVTNSVVNELTKLMNEVVNLYGKLDSETLELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS62</accession>
    <name>A0A1E3NS62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Origin recognition complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_70514</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">ORC is composed of six subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ORC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020034.1">
        <property type="nucleotide sequence ID" value="XM_019163663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS62"/>
    <dbReference type="STRING" id="763406.A0A1E3NS62"/>
    <dbReference type="GeneID" id="30180350"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000199">
        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041083">
        <property type="entry name" value="AAA_lid_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048867">
        <property type="entry name" value="WHD_ORC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF23">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17872">
        <property type="entry name" value="AAA_lid_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21312">
        <property type="entry name" value="WHD_ORC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82061">
        <property type="entry name" value="BAH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAH" evidence="12">
        <location>
            <begin position="44"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="278"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="769" mass="87784" checksum="D35F2178442E65A8" modified="2017-01-18" version="1">MAKTQKDMKGWEILLSEKAESRHLRQKISSKSSGITLRRTSDGLQLKEGNCITFDLNRNSDYKIFGYGFVKDISFAVDKFLAVRILWLVKFDETIAEKLPALPAGDKYFSNELFLTPFLDSVMVSQIINKFELIGVKELKALVDSQNSVDPPKMENLFICRRFTDDSSTYFTDIIEWTTMFEAFKENKDEFYDSVKLLTLKPKSTGKKTTKFTSDAEKSLSRATIAKKTSKRKVELQSEEDEDCVEGEESEDKLADFVVDESDEDAFLEADYSDDDEKRIKKRKKTRSPRKRITEKSPRKSPRKWTRKLNVPELPMIVDEMDLQADGGFSTAKMLMKAKKVLGTGAELKALPCREEEFYQLYHRLEDAILSKNGCCIYVSGTPGVGKTATIREVIQQLSAKMTIESNGMKMFNYVEINGLKLIKPQSAYEVLWKKISNTVTSANNALTFLQQHFEPADEEEEKEDEYMNKLPLVVLLDELDQIATRNQAVMYNFFNWPTYQNSRLIVIAVANTMDLPERLLTNKISSRLGLLRIQFTSYSYNQLSEIIKHRLEQLSKHNDKLLIAKDAIEFASRKVASVSGDARRSLMICIRAVEIAEAEFMSKSDAERKELDGKYTVTIMHIMKAVNETSSSPVTGYLNSISFLSKLVLASIMLRKKRSGIAEIPVGEVYDELNNQIHVLLFTELKNRLKAEDLKLLDVVFGQGNSNMSGSVINASLFILKDLEENGILMMQPIKIERNRLVRLNISDDEILNSFKRDSLLKEIITFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS71</accession>
    <name>A0A1E3NS71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020007.1">
        <property type="nucleotide sequence ID" value="XM_019160367.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NS71"/>
    <dbReference type="GeneID" id="30177054"/>
    <dbReference type="OrthoDB" id="3998287at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="644"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="761"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="895"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1172"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1235"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1320"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="761"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="909"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1117"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1198"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1243"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1320"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1385"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1398" mass="154177" checksum="47CF14FA5F0703EA" modified="2017-01-18" version="1" fragment="single">MGFKDFFSKGPTGRNDGVSYNQGLNTSYSSATNRRYMENMSANGLQSTYSLRQQQPHSSGRAMSLSSRSFRPSEQPRTMSMRSGVYSQQSGSRAMSMTMSSRPNRPPSVSSRANSLTASTRAYQKNPGSRLNSMSGSRAGSRTGSRANSLTPATAGNPSHTKIIKTTKEQDYSGRTKSITTTTIEKRGNMKIVRTTVIHPSDHRINEDAELEELAELEDFDHDATLDDYRHNDETANYHETDGHFDHYDYQNQYDEDYSDMIVNENGGHGENYNERDYYEDPARNGAALAAAVALNGSQRYPDRQQPRAQAQRNTRPKAPGVSQRVRKNGREFQNYDSKQEVRFGESKIVDDLQSDNYDYPHDYQNEVDYELNQQQYQPAVARKAKNKSTKVSKQSSKPVNASNINSRSAQKNQAITAANTPSTGAPIPSGRTRKLDPPQSTRLQQSPISQKSLDAEGIPSKKTIFPTENDVMLHEEQILSPAQSSRAHFNDVANDVISDEIYEDNVGEYDDEEADDDEEVMVVTPELSSSESFKERAQKRLSEIAEVTETYDDDEANVEEDQDFMEEPVLNPLGLDTAISAGVNHKDKTFKGRATLKENANPTMDGPSPFVKPAFAENAGTTTSSITSDENFVEAQEDLVSNPPAITNINDKVGIPKKQSRPLNNTSSQPQPSHTYRNLLFNESKRSPIKNSIPNFNGVHNDYVNDDFDNFPQQISAVSSQSNSPVINQNNFNSFDQNEDDLYTSPPERQTKKLKLKSALKNSSSSLASPISETNPQNGRYANGQRIRAQSKNAVLSPSETRKELTPEEMYALALKAAEKKVYGDRLNSVYPDNDIDDNTKLNTMVNMQGPPGTESAHISNRDSAVLDIPEQGHSASQPGLPYKSNATGLGFRVHSLRDGNSSSRQSKKTDPQEASRLKKFFKLEQKQQRKQWEDERKAAAEPRLVQQAQQKVEKDVSMMPVNPDVELHILQQQEQVRLEQNKLEQLKKQQYQHQQMGTRDSNQAQQFESSQVSNAPPAQTDIFSPTTEPHTATQGVAQNPQQLSAPKVQEPVAQHLMQPAPNNEPVTEATEKTTAVDASPNSSSATSSPNKLRMKMFSFGKKKSTPDHYTHKKQTSLVSQETHRSNASVGRRGSVDGKRNKFFSFGLGGSSHDADIAPVQGVNQIQKVKSTSGQENAGFELKDPNSDLVQTERPVPNSQNVPEAQQDKSVSNAQVYPAAGEVGTAQGIVNDSIGPEVDHQGSAQPQTAWSQNDQPLQPAEITTIPEDVNDTPTALGNIEEENFSQPTYPGAKITNDNFVRGPQGSSSNLIKVGTVNSSVTNGSAYTNNNRSAGNRDSVNPTESSKLPNTEAQNYSLSSPGVFNAKADYAQAPNGETIEVAKQQPVTESSSARQKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSI8</accession>
    <name>A0A1E3NSI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14521</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020133.1">
        <property type="nucleotide sequence ID" value="XM_019160411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSI8"/>
    <dbReference type="GeneID" id="30177098"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03338">
        <property type="entry name" value="TCP1_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000010">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012717">
        <property type="entry name" value="Chap_CCT_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02342">
        <property type="entry name" value="chap_CCT_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="528" mass="56938" checksum="9AB63E3F44403E98" modified="2017-01-18" version="1">MSAAVAPSNAKFKNKEKPQEVRKANITAAKAVADAIRTSLGPKGMDKMIKTKTGDVIISNDGHTILKHMAVLHPAAKMLVDLSGAQDVEAGDGTTSVVVITGALLGAAQNLLDKGIHPTIISESFQRAAQRSSEIFLDMSHEISLDERESLIKAANTSLSSKIVAQHSGLLGPIAVDAVLKVTKKDAHNVDLNDIRLVKKVGGTIDDTEMIDGVVLTQTAVKSAGGPMRMEKARIGLVQFQLSPPKPDMENNIVVNDYRQMDKILKEERAYILNICKQIKKAKCNVLLIQKSILRDAVNDLALHFLSKLNIMVVKDIERDDIEFISKSLGCKPIADIASFSESRLASADLIEEVESSGSKIIKITGIKNVTHPTVSVVCRGANNQVLDEAERSLHDALCVIRCLVKVRALIAGGGAPEIEVSRTLLKEAHKLSGVEQLCLTEFANALEVIPTTLAENAGLNPIEIVTDLRNKHENGEREAGISVRRSGATNTYSEHVLQPVLVNTSAITLAAETVKSILRIDDIAISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSL2</accession>
    <name>A0A1E3NSL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 16">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11 16">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_14550</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 16">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11 16"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020175.1">
        <property type="nucleotide sequence ID" value="XM_019160426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSL2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSL2"/>
    <dbReference type="GeneID" id="30177113"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14385">
        <property type="entry name" value="UBA1_spUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14298">
        <property type="entry name" value="UBA2_scUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000086">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000396">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033864">
        <property type="entry name" value="UBA2_scUBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF664">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11 16" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 16" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 11 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="20">
        <location>
            <begin position="155"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="19">
        <location>
            <begin position="303"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="588"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="653"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="575"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="747"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="198"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ49062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="793" mass="89110" checksum="4A472D7326F99335" modified="2017-01-18" version="1">MDEEFPIKLDSIVVPTSSSKIFKDDCMFSFDTALDPVGLDICMTCHQAFSRGEINYTQMHSEFYDHDLFLNYRKIRKVQKNEEQPYKMIKLEVKEASESELFDSKYSIYDQKLNKSVEYPNKELPIKVSQAANAIITATSSAKKEEIMSWEQEIKPCKHSLAIVQEPIPGLQLKKCTNCDLTENLWICLHCGNLGCGRNQFGGVPGNSHAMAHKNEYPDHAVAVKLGSLSSDIADCYCYSCDDEVKVPNLGALLKTFGINITNFVRTEKSLTELQIEQNIQWDFKMGSDGGEELKPVFGKGLTGFKNLGNSCYLASALQLLFSIDSFQKAFFIPNGVPLDKILGNLKPYEDLETQMYKLGDGLLSGRYSVPDMTTSETTKYQRGLKPSGFKTLIGEGHPEFSTMLQQDSFEFWTYLIDKLEKKKVFGQLEDSPTNALKFILESKIKCNTCQGVRIKKEVTDNMSIPLNVNFVEKDAEGNKIFKETSMGESMDKWNSPEVFEYSCPKCGGKQVATREERLKTTPEYFVVNPQRIILENWVPTKVNVPVKFEELLDLRKYQRTGLLPGEEELPEDATDVREDDNKASEPEFDTQALNNLFEMGFTENRAKHALYATGNNGAEAAMNWLFEHMEDPQIDEPFKAPEIGGSGASSVSVDAEKLASLTSMGFGEQISTKALVLNNRNVEAAVEWLFSNPDDDGVLEAAAIGSAEKDTELLVKELESSFDKSRLKYELVGVVCHKGTSVHSGHYVAFVKKIVDGEAKWVLFNDEKVVLAIADNIKEIETTGYLYVYKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSV4</accession>
    <name>A0A1E3NSV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019766.1">
        <property type="nucleotide sequence ID" value="XM_019163599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSV4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSV4"/>
    <dbReference type="GeneID" id="30180286"/>
    <dbReference type="OrthoDB" id="3367at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045013">
        <property type="term" value="P:carbon catabolite repression of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF16">
        <property type="entry name" value="AT02583P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="708" mass="78961" checksum="81E9EAE2D5D3B14C" modified="2017-01-18" version="1">MYIPPDLSRYNPKPVIYSQFLASSIYPVQITDPNTVFCVDKEEFRFTCLNSVYEPDVALRIARTPLSPSDPFTYAALSKFTEYYLRKFHSSTGAQSSNQIDKPPLNVPKELIAAQSDGKTEILLVGSLLNPLVSDSYKPVHLTPGCISSIVNFTKDNASHEAPNTLSNKLFGKTRNTKGNVDLNNKQPKTSLNRNSSAFIERIATCDNYMKKMSNAESLLISVHGRVLNIIALDQNPREIEIDSPCLRLILSNGIITCFTSFEYTTTSGEKNLDILFGFATGDILWLNPLRMKYSRWNKNGKIKSGLITSLQWSKCGKFAIAGFADGEVLTFSRNLEDPESAYQPTVQIKERHMRTVRSLKTQQNQSANPVAHYKFSKKPITHVTIHPIFHNVVALTSDDGLLRIFDLLTETITDIVPSYYAGLLVTEFTPDGKYIAVGGEDDIVSIFEFQFLNFFSSPSESGLLKLVTRLQGAKSWIKGIVIGSNGLNSSLSYTIGTASDDGYIRFYEFQPRALRKVKRHAHVASASYIGTPKFQMQRGFSNSSVEPSLDSRKKKLANRSSNTLVSMNNTNNHLSLLEIINQGSSSTSLQQLQPQTSVHLKLGENNMVNSSAQSENRKLELMKINALFKNKTTSAVSILLRDSRFATPHIHTVSGMNTVPNVLPVSEKNVNLGRLSGLHMSKEFVWAFVATGDLIRWKKHESYSNAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSV5</accession>
    <name>A0A1E3NSV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14570</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020199.1">
        <property type="nucleotide sequence ID" value="XM_019160438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSV5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSV5"/>
    <dbReference type="GeneID" id="30177125"/>
    <dbReference type="OrthoDB" id="2193813at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08049">
        <property type="entry name" value="TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037818">
        <property type="entry name" value="TAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor TFIID subunit 8 C-terminal" evidence="9">
        <location>
            <begin position="271"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="328"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="559"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="482"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="559"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="596"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="661"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="671" mass="77453" checksum="279D9485B57F0A38" modified="2017-01-18" version="1">MSEAAASQQPGRPAVTQERATIRINDIPTELLERLLGKYVQQVNENYKKKQGELKIQDFDETKDIEQPIIPKKRVLAEASETIDGAQELSDRMVHLNKTLPLYGYFPDGNGMIEVSTRTDEPMADVLQVAVDQIMTASIRYKHGYSDINIKKSTLSQMTQWTALFFHLLTTRLQRLMEIQRRRLPNSDDLLLLLKEGYFDRKGIDEMHTLCERYYSQNSHRNKELHSIAKHASTAYTGQDYGDFNSANDSVVMEEPWWIDQVIHRKKRKAYIPEWMPPLPPEHTFKSTPKYSQRVTNPVMLREKLVLEGRYGEKALDHIIVKEESTLCSPLSQESISSSDDEEEEEQEQIKGGENNISTGREIEVVQKSTQNEKSDINNDNTELEAPTVVGLDVKSDHERINDSEGSNEKEKTEESTLESATPSDKKVDLVELANKRMAVLENKRKEEEQRYLSRIESDESRFGRNFGFYSNVKKLPDGINVELQEYRNKKLREVVHNLRKQEKKNSKWVIEQEELRRRLDEEKSKYAEANQIQLGSGQDAADENIFYADMDEEVDFDVEFSDMEEVEDGENLPKPPETKTESIEPAVMDNDSNDEERVLSVRFQDELETSALLNSTGDGEERDREGEGEGDIEREDPEAKYRSDMQEEDHDDCDRVVMDKDEEDESVAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTC0</accession>
    <name>A0A1E3NTC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_24953</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020435.1">
        <property type="nucleotide sequence ID" value="XM_019162371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTC0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTC0"/>
    <dbReference type="GeneID" id="30179058"/>
    <dbReference type="OrthoDB" id="4215304at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050226">
        <property type="entry name" value="NagZ_Beta-hexosaminidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30480">
        <property type="entry name" value="BETA-HEXOSAMINIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30480:SF8">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04060)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 3 N-terminal" evidence="4">
        <location>
            <begin position="15"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="917" mass="102238" checksum="9855598154A2A7B1" modified="2017-01-18" version="1" fragment="single">VGNFFIVGFEGTTVTKEISILISKYHVSSIILSGKNFINASQAKSLIQDLQTIAKKSGYKYPLIFVIDEEGGMLNSLFDGDYVTQFPGAMSMGAAGITDLAYKVYRAMAKELKSIGFSMYLGPVLDILKNTSNTFIQQMIGVRAYGYDLKSTLKLGEVAAKAFKDEGVLNCGKHFPGYGSATINSNFELPMIFESADQLFEFNLVPYIELIKENLLDSVLVGGCAVPGVNNNDLHACLSPTIVTNILREKLQFNGVVISECLLLEALDRNFGVVQGCISAFSVGCDLIMLCSNFDIQKQAIMALKSVIEDQLISPTIVNKSLERIQNLQQKLPSWEEVLTTQFLTPDILAYHKLLSKKAYEKSITLIRDAGMPISKYLRAGVENENTVLMLTPLISPLYETVDSNGVKTHINSIVSHDYGNLNLNYGEDVFIKLGELLSSYKPGYKVLHTSYNSNGLTSFHEELILRSKVILFFCAETTNNLYQVGVSKHVSMLCNSMANKKNSLGRSLGGKKMIIISVASPTDFLYDINLGGSPTGYICTYDYTINALCNLPKILFGDFKATGKIPGLSTHVLQNGIVQNSDNKHRKHSWLVETFSYKRDWENLINLLKNNNYLDYSVKDTSLTSLKRLLFDTENHRSFVVRNTSSNTILGISSAWVYSSSDLQVDGKKLNVANLMFLLVDKNKRNISIGNHLYSKTIKYLFEERACGKIYLGRNFPKFTIVNDLLLNFTEENNNALNFFKSLGCDFSGETVIPEVKGHDISSWQVAENLVRQLQVVGIMFDICKNPGDILSSQKGKHSSSNFYSSESDLYEVYLELYKDFCSQNDDTQYFDGSGNLDIIVALEPTKRYVVGSLVVFNGKSKFSKFYPCLESLGSEKLTNQNEYACITGHFIDPLYTTLSEVFKLGLICTALMYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTD9</accession>
    <name>A0A1E3NTD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cys-Gly metallodipeptidase DUG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deficient in utilization of glutathione protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">GSH degradosomal complex subunit DUG1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_70879</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020443.1">
        <property type="nucleotide sequence ID" value="XM_019163759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTD9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTD9"/>
    <dbReference type="GeneID" id="30180446"/>
    <dbReference type="OrthoDB" id="7832001at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05676">
        <property type="entry name" value="M20_dipept_like_CNDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000008">
        <property type="entry name" value="Cytosolic non-specific dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017153">
        <property type="entry name" value="CNDP/DUG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF4">
        <property type="entry name" value="CARNOSINE DIPEPTIDASE 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037242">
        <property type="entry name" value="CNDP_dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="15">
        <location>
            <begin position="220"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="14">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ49330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="480" mass="52600" checksum="7A1765E93570D3DD" modified="2017-01-18" version="1">MSSIDTTLSPLFAKIDELKPQFIQRLTDAVAIPSVSSDETLRPKVFAMADFLKEELAKLGADDITIKDLGIQPPPVSDPELPLPPIVLSKFGSDPNKKTVLIYGHYDVQPALLEDGWASDPFTLVEKDGKLIGRGSTDDKGPVMGWLNSLQAHKELGLELPVNIVFCFEGMEESGSIGLDTLIAQEAKKYFKEVESVCISDNYWLGTKKPVLTYGLRGCNYYQISIKGPGADLHSGIFGGVIHEPMTDLVNVMSKLVDINGNILIPGVNEMVAPVTEKEASLYKDIEFDVDELNLAAGSNTAIYQSKKDILMHRWRFPSLSLHGIEGAFSGAGAKTVIPAKVVGKFSIRTVPDMDSKKLDELVINFCNEKFKELKSPNQFTTELIHDGAYWVSDPFNNEFTAAKLATEKVWGVEPDLTREGGSIPITLTFEKELGANVLLLPMGKGDDGAHSINEKLNTPNYIEGTKTLSAYLHYFGATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTE3</accession>
    <name>A0A1E3NTE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LsmAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020484.1">
        <property type="nucleotide sequence ID" value="XM_019160561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTE3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTE3"/>
    <dbReference type="GeneID" id="30177248"/>
    <dbReference type="OrthoDB" id="3998162at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045117">
        <property type="entry name" value="ATXN2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009604">
        <property type="entry name" value="LsmAD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854">
        <property type="entry name" value="ATAXIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854:SF7">
        <property type="entry name" value="ATAXIN-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06741">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01272">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LsmAD" evidence="2">
        <location>
            <begin position="212"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="501"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="586"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="763" mass="84069" checksum="0218E0907987F0E2" modified="2017-01-18" version="1">MQNKPFPKVQSAQGSYSNNGGNGSQNGPNAILSPTEYDAANKHMKDRFIYALANSLGTKITVTTANSDKITGILQSISDVNDTIPSLILKYPTRAGHVYDDEFLMIPESSISMMTCENLDFTPKASASSASASASVASSTSSSSAHNHSAARRGFRIDTDISSNDTYRERERDLQKWVPEEDDSTSLGAALEEEKFSDANKWDQFKTNKDKFGIDAKFDENDYTVKLNVSNPKFKERMKFAETVSREILSQSSNGNLHIAEERGKLVDAENIDEEDKYSGVLRDNAESEKKLMNMLKTNKPDSFSKPAPAAAAAAAASTTVAAPSNKLSGQYIPPNQRNAQTQKDVSSTAGTKRLPQAPVSSTTPVATTPSAAATPVQLPEKPATTQQTKDTSKKSANNEPAPSAKKSAAAVPKKPAAIPATLTLSAPSTTSTIPTNSANSSTSTATPATSTTQHAQKSQTQAHQPGTQTPQLSTQQNGVPSKQKSPQLQKQNFTDPTLDQNQNQNQHQQQPHQPHQSHQPHQIHQQHQHQQGIPQQLPPQMQQQQQQQQHMMRNQQFGYQPRSNIGSQQQYGSPSMQYRNHHNGSNHSSSQYNQNYANSNNVNANANQRMKKSSPQFLFSHRPSDKAPRGSVLKDRFNILLSAKKEYKSNSANVTIDIIPMVPSFHTSPSWPETTSESYSEIFTKENNSHPNMHMQQSVYMHPQAYPQNHQPYMGFQVQPPYYFPIVPSYPVYLPNVAFPMMGGNQGMDKVYPNHARHGNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTG7</accession>
    <name>A0A1E3NTG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_89515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020071.1">
        <property type="nucleotide sequence ID" value="XM_019165047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTG7"/>
    <dbReference type="GeneID" id="30181734"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="2">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="504" mass="56064" checksum="AFBFE16B035DDAC0" modified="2017-01-18" version="1">MVGTEPNGNYESLWLDSKNHSQQAKNVALKLMQSEKFQTKLMKLIQHFDSSTPKDTHNHPSTASSSPLRPGHAKRSISGSSISNPISFSHVSHVDAHSNFGLADSFNPLNLNQIDRCLPAHSLHRSSIACSNNDTSYTTEDSDADPFESTLDPATSGIKAFSTYRTSPTYTTHSIIPSHSGYSLSNRLHRRTSSASTNFNVTSSQFSTNSIFTSNNAGVCSSDTSRGSSPRNSLKVKVLPCIPQEDLTDRGVGHERESEYSEPLMEQEEDEENPFLEKFVSIPSAPPPPKSVEAPEDRLKSFLQREEIEIASDSEDDDICFEKFRLSSNIESITSELQAQLQKRLNSENKIMNKEYRKSIMILLQKEVQEQSLLEDLEEEEDLEKSFFEGSPSPKHNARRNPSTLLSRMEHIEEELEADDEGEDDDTEGTSANDNCSIMKSLALADSTSLIDTAFELDAETEEENDHRVSAAAKRLSVLSDFFADLQLNETTLLDSDDRALLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTP9</accession>
    <name>A0A1E3NTP9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020577.1">
        <property type="nucleotide sequence ID" value="XM_019160593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTP9"/>
    <dbReference type="GeneID" id="30177280"/>
    <dbReference type="OrthoDB" id="10637318at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="187" mass="19729" checksum="256BEEB8757024BE" modified="2017-01-18" version="1">MSGPDYVYANGNGNGNGSANGGGSGGNRRRSSHNANGLSGGGLDSVYQPVNLFNGVEIQNILHNNFERYRSKAAVYTESVGRASAGGGEAARPSEEHEFTMVSSSNSGANANTAATATATANSYGGERYGYDRAGYGGRKGKDLLSELSKSMMHMHPHTHKNSASSASSASASHYHYHHHYQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTR9</accession>
    <name>A0A1E3NTR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_27905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020553.1">
        <property type="nucleotide sequence ID" value="XM_019162521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTR9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTR9"/>
    <dbReference type="GeneID" id="30179208"/>
    <dbReference type="OrthoDB" id="1106148at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="14075" checksum="D204A2E0F06B9B76" modified="2017-01-18" version="1" fragment="single">SSQSRSASTMAAPAAAYRPPTQASAAHPVTSPAAAAPQQPGMFAQMATTAAGVAVGSAVGHTLGAGLTSMFSGSSSNDAPAEQQMQQAPAVQSQGYNSQQSAPCDVDARNFTRCIEDSNGNYQACDFYLQQLKACQAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTT6</accession>
    <name>A0A1E3NTT6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020674.1">
        <property type="nucleotide sequence ID" value="XM_019160626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTT6"/>
    <dbReference type="GeneID" id="30177313"/>
    <dbReference type="OrthoDB" id="10488818at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="303" mass="34928" checksum="F65382BA7DB98A44" modified="2017-01-18" version="1">MEERKDSTARHIRSFGDLHLAAANVYACIDVYDLFRRVVNMIASLEYSYMYLVVLRSVITEAPQKKLLCSKWHKLEKSVIQVPFRTTVDVLTDVTYVPLLSRNNCEILKILILRMNVGGSGKRMQDEKEAENDEKETPMPVLLLSPSVDESHFMPPVEPAEEFTGKSIFKSKRRPFQIRWNHEGEYDIGRLHKKYGSNLTDDDLDILTDQFLWPCLKLTPAMSLLDKLSVILTGYLYSGELESHTLQRFWEGRSQAALTNNENLRNSKIPAVVFEDLDFKTAEASLTELKLRMTAKMKEFEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTU6</accession>
    <name>A0A1E3NTU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_30574</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020684.1">
        <property type="nucleotide sequence ID" value="XM_019162640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTU6"/>
    <dbReference type="STRING" id="763406.A0A1E3NTU6"/>
    <dbReference type="GeneID" id="30179327"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="122"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="319" mass="36886" checksum="FBD123C0753731DE" modified="2017-01-18" version="1">MSTEIQEADVDSIIDRLLDVRGSRPGKQVQLQENEIKYLCTKAREIFIQQPILLELEAPIKICGDIHGQYYDLLRLFEYGAFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIIDEKIFTMHGGLSPDLNTMEQIRRIMRPTDIPDVGLLCDLLWSDPDKDIMGWSENDRGVSFTFGPDVVSRFLQKHDMDLICRAHQVVEDGYEFFSKRQLVTIFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPAEKKKYQPIASSKKRPGRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTZ0</accession>
    <name>A0A1E3NTZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Striatin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_104580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020665.1">
        <property type="nucleotide sequence ID" value="XM_019159672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTZ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTZ0"/>
    <dbReference type="GeneID" id="30176359"/>
    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF0">
        <property type="entry name" value="CONNECTOR OF KINASE TO AP-1, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="4">
        <location>
            <begin position="19"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="36"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="251"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="688" mass="77143" checksum="D769F6DC5AACA083" modified="2017-01-18" version="1">MQNGAMGHEQGMSSSANYTLPGIMQYLQSQFTQVERNRLQGELERSSLKLKIIELENERNSLLRKNAKLQLTVEQLTEEIDQLKGSAVEDGTNSSKTTKVASTEKSASNSEVEQILGGIHSLDVSKLIHARKFLKSATNEILYLLKTPTVELENTSSVGSITGISSLTGGDNDNDMFFNYSTLNNVAQNPTTNGSFHFNNNKSTNINANEHFEKPNVEKELNEILAAPFKSTGNSNSEESDAETIIEESNEEKNSENQKDARNVKKDIEDTKNEDGDNQHENDDDQAEDDEFDDEFDDEFDDDDEDDEDGDDNEDGYDASDDTNGETEHMSNSGSRKYPSKRNIAPGSQHGKNQKEKLLNLLKKSPKKVNSSTNRTLSVKLHPHLSEMELKSGRLFMYYDKANIVKIYDDLLGKGNLIKEIQLEDANKIVDIITNENFIVIATTDSLIAYTIDSEIDLKITKAQVGAKSIDLDKDKLLLVSNSQIELLKIDLVTETFKELSLTEYQYEGTLKKAKFVKNNSSFDVAVLSSNTLYLYNTDSTQAKSKPFKSIPLNQYHEWLVTSRNMILRFNNGLYLFDFSECSEFKQVTLPPLPDLQSLSESISEVISPCVDDDTMFYVTRCTNTPSNNSLGLFELRLFKVIETGDILDIKSLTDLSGLERYCVGKIGDGFSICVARGKEIQLYDISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU26</accession>
    <name>A0A1E3NU26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein MUM2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_75668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020176.1">
        <property type="nucleotide sequence ID" value="XM_019164940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU26"/>
    <dbReference type="STRING" id="763406.A0A1E3NU26"/>
    <dbReference type="GeneID" id="30181627"/>
    <dbReference type="OrthoDB" id="21221at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="335"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="417"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="233"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="62766" checksum="04CE2E647797090E" modified="2017-01-18" version="1">MDSSLLIASSSETTHSLSARSSLANSLYQGSYDPYKAINSNNSFTTSPTTTKKNSVGSVNSASFYNFNSNSNTQPNWNTNNTNNNSESTIKASAILEASPTTATSVTNSINSLPHNMDIWNSASGLTADSALHLNLNSNPNSNPNSNPTSNSTSNLTKNYDPVYSLNTDSSSLYKPNYSNVEMDPLAYQMLQLSTKDSISQLTTPSLIQNTYFGNSNVNYNRQLKESKNQLFNNNSNSNSNNNNNNNNNNNSDSTNNENSNLADNNWSNTNSENANNTYNNFLLQQKHQNSFSNQYTYKPPKQQMQASNLIHPQSQNMNMNMSMDTNYDQTKQNQSYIHSQSHGHFNSDYNNSNSNSNNSSNNTATNNNINMDNKAYNIYDSRVLKMNQSKSSDIDPPEIENYKTKIQLKDIIISKLELELEKLKDFQNTILKTQNESSNSFEIPKNHEELYHKLVEKVRSTEAELEDTKTRLESLITAISMNSTSSSFKSGRYDEEEISHKIISKLKMLTEENDELSRMVSYGKSKEKDIEIALLRKQNNDLLEKVSKLEAKLNDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUE9</accession>
    <name>A0A1E3NUE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_21065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of SLM1-SLM2. Binds phosphatidylinositol 4,5-bisphosphate, which is required for function. Interacts with the TORC2 subunits AVO2, BIT61 and TOR2. Interacts with the calcineurin catalytic subunits CNA1 and CNA2.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020316.1">
        <property type="nucleotide sequence ID" value="XM_019162184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NUE9"/>
    <dbReference type="GeneID" id="30178871"/>
    <dbReference type="OrthoDB" id="5598057at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13311">
        <property type="entry name" value="PH_Slm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000328">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000078">
        <property type="entry name" value="Slm1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043453">
        <property type="entry name" value="Slm1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF16">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE-BINDING PROTEIN SLM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="372"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="196"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00064463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="501" mass="57685" checksum="1842FB517950E227" modified="2017-01-18" version="1" fragment="single">MSLVSQEQLLQNQLQLKQLENQRNKQVQQSKSLDSSVSTNRSSVAAVAMANNSASDPLSITVPTNANPTEILAARFNSWRIIISALSHYLKETVSVHEEITRQQIRLHHAISFPFLTQGLDGEFYQPLRVSNETSHSGTGNGPKTSEFELAKKFFLPLGSGSIQDLPTILYQYHSNAALLAQSTVKELSATIIPRLEDLKRDLLVKIKEIKSLQSDFKNQVDRYQSETSRLLSTYVRSIDFCKSSPSQLDPKNDPYLIKFALDRSIRRQLTEENYLHEAFVNIQTSAKELEKVVYIELQTALTVYAKLLGQQAQNVFDGLISKLDSTLLTKEPTLEWDSFIDRDHSNFIELDLPMRRVSDIRYKYQHEALIYEIRSGWLERKSKFLKSYQRAWYVLTSSFLHEFKSSDRRKDPIPERSLSLNELTVSEHSRKDEKNPNSYHKFVIKHGQTAGLLHKGHNWVFRVESYDLMMAWYNDIKRLTQMSGPVSRASVALERKRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUX5</accession>
    <name>A0A1E3NUX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear distribution protein PAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Lissencephaly-1 homolog</fullName>
            <shortName evidence="11">LIS-1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">nudF homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PAC1</name>
        <name evidence="11" type="synonym">LIS1</name>
        <name evidence="13" type="ORF">PICMEDRAFT_14808</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. Plays a central role in positioning the mitotic spindle at the bud neck during cell division. Targets cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-mediated microtubule sliding along the bud cortex and consequently the movement of the mitotic spindle to the bud neck.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Self-associates. Interacts with NDL1 and dynein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Spindle pole</location>
        </subcellularLocation>
        <text evidence="11">Localizes to the plus ends of microtubules and the mitotic spindle poles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020456.1">
        <property type="nucleotide sequence ID" value="XM_019160543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NUX5"/>
    <dbReference type="GeneID" id="30177230"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="194"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="240"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="414"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="504" mass="56656" checksum="26DF7CBF67451F48" modified="2017-01-18" version="1">MGDNRRIFSEGHVSVLRSLPSSFLSHKANLLSPRQLLELNASILQYLKPILKSEPEIYSILESRLTNHGPHGANDEQITKSIEIPENYLQKKWTTVLRLQRNIQELEMKNKALEGRNKEIVEMLDNEVKANPHCSISKINWIPSFLKSSLRFHTSPITAIAIHSFNPYIVTASQDGMMVIWNLLDLSEPVNQLKNAHSKSINMVTFQKGTSYLISCSSDQFIKVWDLSTPDDVRIPIKSLTGHEHIVSAISSSHKDPNILFSCSRDKTIKVWDLQTGWALRTIDAHSDWVRSIDSSGDYLISCSSDTSIRLTHWPTGTGVGLCLGHKQVVEDVKFIPKNSNEYLDAFLAKTGNKGTLSSEQKTEYSQVGYKYAVSCGRDKLIKIWKLPLPDYNTLNGSPMANSLNPYGECIKEIKGHASWVRCLQIHYNGRYLISSSDDQTIKIWDLSKLDEEEVKPVKSLQGHQSFVNTIAISSPTDDGELSDDKIRCYLVSGGADNVVNVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND88</accession>
    <name>A0A1E3ND88_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015179.1">
        <property type="nucleotide sequence ID" value="XM_019162053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND88"/>
    <dbReference type="STRING" id="763406.A0A1E3ND88"/>
    <dbReference type="GeneID" id="30178740"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="531" mass="59647" checksum="AE18D83D2C9F2FFD" modified="2017-01-18" version="1">MTIDPPQTFSVKSVAIIGAGPSGIASLHDLSRVTRSGKSLFGERDISKYEKEGDLAFPDLVAFERNASVGGVWSKSAFAENNRDPNLPEFKDDSVDLTNPENVYQKIPIDAGLEKKLGDSSVDHPVKVLVTPEISTKIQHQWRSSAAYNGLFTNVTNRYMSFSFDERLGDDLKKINSKYKHIPDHQSSRDVSDYLERVVANNNLEKYIRFNTNVERVKKLPSGKWEVVASHLAEENGVQYLHWYKQIFDAVIIGNGKTVPIVPNIKNMAKFAEVNKDKVTFKLAKSVQDPSFIRNAKKPLFIGSSVSSVDLIQYAFPRDLEKPSVYISRRTETANSGWVMFCSYAKGVVNKPTIEEFLPESNSVRFSDGTVESGFDAIIVCTGYHMFYPFIDKSVVDSNPNIFKFFRYTFSMADPTLALVGNTYAGFFFNRVESQAAALAGVWTNQSKLPSLKEQEQEYEQRPGLLVVPIIDKRFIRPLISLAIEGRPHPFTVNKEKSDYIYHVATGGHTILGLFFKVRNGEVDSRDLLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDH0</accession>
    <name>A0A1E3NDH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maltose/galactoside acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_20568</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015277.1">
        <property type="nucleotide sequence ID" value="XM_019162162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDH0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDH0"/>
    <dbReference type="GeneID" id="30178849"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF23">
        <property type="entry name" value="ACETYLTRANSFERASE C18B11.09C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="183" mass="20252" checksum="1FB52636E39BB1DE" modified="2017-01-18" version="1" fragment="single">MIAGELYSCSDPQLVKDRYAVRRKLHRYRMLLEEKTLAEDFLHDHVFREFADCSTSFIEPPLFLDYGYNCTIGHHFYSNFNCVMLDCAEITIGNHVMFGPNVQLITATHPKDSVSRNELCQEIAKPITIGDGCWLGAGATVLPGVTLGKNCVVAAGAVVTKSFLEDGLVLVGVPAKVTERLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDJ4</accession>
    <name>A0A1E3NDJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylenetetrahydrofolate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74807</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015312.1">
        <property type="nucleotide sequence ID" value="XM_019164877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDJ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDJ4"/>
    <dbReference type="GeneID" id="30181564"/>
    <dbReference type="OrthoDB" id="41403at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000012">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="2">
        <location>
            <begin position="13"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="3">
        <location>
            <begin position="146"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="36794" checksum="3E0D2BA2267F011D" modified="2017-01-18" version="1">MSAAIKACKVIPASKIAKTYLQEVTDATARLARKPKLVGLLANDDPAAMMYATWTGKTANTLGFQYELIQVSKDQLEEEIYKCNNNDDINGMIVYFPVFGNKQDQYLQQCVSIHKDVEGLNFKYISNMYHNVRFLDEAKTMKSILPCTPLAVVKILEYIGAYNKVLDNGDRLYGKTITIVNRSEIVGRPLASLLANDGATVYSADINDIQLYERGDGLRYKQHKVLDCNKSLEECARISDIIITGVPTKTYKFPSEYIKRGAIVINFSSEKNFVDEDVEKVASLYVPSIGKVTIAMLLRNLLRLMDNKKAVNAKSVEPPTAPTDKPQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDM5</accession>
    <name>A0A1E3NDM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uracil catabolism protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015327.1">
        <property type="nucleotide sequence ID" value="XM_019164881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDM5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDM5"/>
    <dbReference type="GeneID" id="30181568"/>
    <dbReference type="OrthoDB" id="2153176at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012469">
        <property type="entry name" value="DUF1688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687:SF3">
        <property type="entry name" value="PROTEIN URG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07958">
        <property type="entry name" value="DUF1688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="47069" checksum="FC2D3E0AA07BE6DB" modified="2017-01-18" version="1">MTIPEEIAYLYSIRSVRETNSSTVALIENNKLLNFEVDLGKMEEVVDFVVETIRGTFPTNAALLEIPVHGRWQHFGKGNTSRLPDLIEKVFRQELGLDDEEVCRKLIDLFVVSVLLDAGAGNEWKFTEPGTGMAISRSEGIAVASLYMFQGGVFAAAAGGGDPYVVQGSRLASLGAEELAAGLQVSQEQNPMSGFDGRLRLLNRLGEVLSSNKELFHSGRPGDLVDYLKARSSGSGSGKAVELEVLWDALMQGLLPVWPTEGRTKIDGYVVGDAWPLQNKAGGIVTFHKLTQWLTYSLFKPLTEYGGFSILHAECMTGLPEYRNGGLFVDLGVLALKPAAHARGLALAAQHDLLPLPAFRPDDDAIVEWRSCTVCLLDMLLPLVNAKMAVAGTKYELTLPQLIEAGSWKSGRLAAAARRPSGGPPIELVADGTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDP5</accession>
    <name>A0A1E3NDP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015347.1">
        <property type="nucleotide sequence ID" value="XM_019162016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDP5"/>
    <dbReference type="GeneID" id="30178703"/>
    <dbReference type="OrthoDB" id="4091811at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="82" mass="9544" checksum="F149AA326A40FFB5" modified="2017-01-18" version="1">MPFLVTYRPAILLFFFIRQPVAAASLVGMFALWEADRNLGLGIRESFNEWRNTALGFATVQSQYDAFKRIDEQKRNEMLVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDR3</accession>
    <name>A0A1E3NDR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein-sorting-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ESCRT-II complex subunit VPS36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18517</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the VPS36 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015388.1">
        <property type="nucleotide sequence ID" value="XM_019162037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NDR3"/>
    <dbReference type="GeneID" id="30178724"/>
    <dbReference type="OrthoDB" id="271448at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021648">
        <property type="entry name" value="GLUE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037855">
        <property type="entry name" value="Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031558">
        <property type="entry name" value="Vps36-NZF-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128:SF12">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16988">
        <property type="entry name" value="Vps36-NZF-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11605">
        <property type="entry name" value="Vps36_ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51495">
        <property type="entry name" value="GLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GLUE N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="641" mass="72949" checksum="5A743BF10760276C" modified="2017-01-18" version="1">MPTQFTTPYFKHVDLSSSGRPILNDHEHDIIMRNGIGLYQEDYKLKNYQCGRLYLTNQRIIFVNDNHRDRHVSLDLGEIDGTEIYNGFLKSSPKIILKLKDQDENEMKKSQRKNELTMAWVCPICSYSNESSINTSEIENMKFSDKNRPACNTCGVKCTYDVINKYYSSNINTTHNGSIDLVSFDGSQCPNCTFINHPFMKKCEICGSQLVGSPSASSSELPIQNQSQKLIKFTLLEEVSDAINLKVVKFSFRNGNQKLFYESLKKVLEDRNRQSESVGDNQEKSSGHTKSDTVASQLTFSNGIHGLTANSKQKNYEDSLLLGKAVQDLDQLMSKAKDLISLSKKYQNFLMKTSKHDKDFDQNFELLERSKSSVANLNHIIDNNKIGESINSAKITNALNLLRSGKPSTNTISKLPTVYLEELARNICDFLINDDILDKKNGIITFFELYSMYNKTRQINLITPEETFDAMMKFEDLRINIEVTKVLLSRKEDVKSSPFFYIVSKKNNSSSITAKIFNYIIINPGISIPRLQKLHFNMSFIILKTILDNLVYNGELAIDITLEGDTYWPNEFLNIARNDSNHPTIVSKDVQIDGDNLEQHQKIVSNSSAISDLSNIFKASHIYSSDITSERFKELKDLSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE08</accession>
    <name>A0A1E3NE08_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Suppressor of lethality of KEX2 GAS1 double null mutant protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13564</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015472.1">
        <property type="nucleotide sequence ID" value="XM_019160079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE08"/>
    <dbReference type="GeneID" id="30176766"/>
    <dbReference type="OrthoDB" id="4082885at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="48258" checksum="5EFFFB0607DE3376" modified="2017-01-18" version="1">MYSTTGLAVGMAIGVPTFVFVVVFVIFWYRQKLKYKADLKQHNGIDDYDEVNDLDLDHMIDTPKESHVKDFSTDELVADGGQRAVTGDSGVDSGVAGSGVLIKNGRYSIRKESKIMGLRIVPKNQREPAPPRPIHTGTTSNSNSNSNSNSNSNIATPLEDTHRDLSTNSNPSGSRAYSDLRGHTPVDNDNNYKVYYESVIPVLPGSGLEQRASGSFDGQDSAVNAPNTPMKGGNSFARNSNHSGTEFYKMLQEDSPFYPRSRITSFNPGETPLRKSSVPNVASSVNSHDFSKTHRSQQQQQQQQQQQLFHSPSQSSTAEVSPFDTPPARTRVSERLEREDSTTTFDHPTSSSAHDADHVKDTASNLFDHAMDSIDQSDADDTSNHNSALESSPLRKNMHRRINSAESRIILDGKDTAEENYNIKRKEWLDKYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFB9</accession>
    <name>A0A1E3NFB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L21-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_18200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015935.1">
        <property type="nucleotide sequence ID" value="XM_019161911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFB9"/>
    <dbReference type="STRING" id="763406.A0A1E3NFB9"/>
    <dbReference type="GeneID" id="30178598"/>
    <dbReference type="OrthoDB" id="1539250at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3260:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17559" checksum="05F8380A5E70B0EB" modified="2017-01-18" version="1">MGKSHGLRSRTRYAFSRDFKKHGAPAMSTYLKTYKVGDIVDIKVNGAIHDGMPFKFYHGKTGIVYNVTKSAVGVICNKVVGHRYIEKRLNIKIEHVKHSKCRQEFLNRVKENSAKRAVAKASGEKVQLKRQPAGPRSSRVVSGVPTTLAPVAYETYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFQ3</accession>
    <name>A0A1E3NFQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_68973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016080.1">
        <property type="nucleotide sequence ID" value="XM_019163511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFQ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFQ3"/>
    <dbReference type="GeneID" id="30180198"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF78">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="33"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="530" mass="59349" checksum="55B143A79CE9D6E2" modified="2017-01-18" version="1">MSTTVVEREAYNVRQLVAASHENILTSKKLIIVFVSMSVALMISSIDLTGVTIAVPYMAKDLDAYSSISWVGTSSLISTTVFMVLFGRFSDIFSRKYTMIFAMVMLAFFDLACALAQTPTQLYVFRALCGMGNGGITTLSMVIVSDVVTLEQRGFYQGILGAFFGIGSAIGPFIASAYINHSSWRKFYFTLFPICLSSTLILIKYVPYTRPQHSMKEKLLNLDYLGFFTSSVAIIFLLIPISGGGSTFPWTSAFTISMIVIGGVFFLIFLLVEHRVSKLPLIPLRLFNESFSLTSILLHNFFFGICYYGIQYYYPYYFEVVRGYTPMETSCFLLAFVLPQTLTSICSGQLISRTKHYGFVIWIGYTLWMIAMGLLSLWGLNTKKGVTIIIMVINGLGSGFIFQPTLIAAQAHSLKRDRATVISTRNVLRTFGGSISLAISATILANTFAENLNATGSKYFSKTEIEVLKTMIYTKPDLSKYTSTQALYLKEIYMLGIKRIFYVWMACMAYCFITNMPIKDRGLQPIDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFT2</accession>
    <name>A0A1E3NFT2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_157126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016059.1">
        <property type="nucleotide sequence ID" value="XM_019160931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFT2"/>
    <dbReference type="GeneID" id="30177618"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="90" mass="10366" checksum="C949D9EC31165C02" modified="2017-01-18" version="1">MINHKLWSKHTILIAGLQISVSTGSMHRYSMLSPMSIQMLVVGLELNIVEGRMSMDHGWQIIMYLRLRKVPVLKFMMARGSERLATFESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFX5</accession>
    <name>A0A1E3NFX5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015366.1">
        <property type="nucleotide sequence ID" value="XM_019162028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFX5"/>
    <dbReference type="GeneID" id="30178715"/>
    <dbReference type="OrthoDB" id="3997496at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="297" mass="34719" checksum="0714D2272C77532E" modified="2017-01-18" version="1">MRRHVSNLHTLSLLDPYTASRLSSLLLKGESEALRLQRLLAGQSKRSKQRSNTISAILHYIYHHSSVLSRAYPAWMFRFAFAKTVSKKDRLRFVQEYSPMLSFSHTIMRIMGKEQNDAALRDKYIAFWKSTQLEFVKSTQFLDPEVPLETNSDKILQLKFQPQTPVSEDVRRLIKVRQQYSSVVKPKFKLPELVISVDPNRFGLPPSAERIFNMRLARLVKLQKYYRKYPPITQEDMHIFNQLDLISFPGAVREAYVNFLSLTAFTIDDEARIAKSNILEKRIAISEKMETLYKELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG73</accession>
    <name>A0A1E3NG73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_59931</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016232.1">
        <property type="nucleotide sequence ID" value="XM_019163402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG73"/>
    <dbReference type="GeneID" id="30180089"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14474">
        <property type="entry name" value="SPX_YDR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="838"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="874"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="913"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="935" mass="106489" checksum="A19F0DA9FE942E3B" modified="2017-01-18" version="1">MKFGVEFASRSVSRWRNYNIDYNLLKTLIREATSGFEDSSSSDNSTATLTEHQKKLLQKLYKNFKDQIDFASLFVFSKVGEISRRLSMLKKQCNIFIKSETEYTAEHPQSDVSLRLRKRKLLLFHKELDSITNELQDLSRFILLQKIAVKKLLKKFVKYSSYPQKQSFVDKITTKFLVENPKSFIHLSLDDLALETTLLYDFLDTFLTTCTAESNQRKHRHSSIHTIDSLQLVTQSYSINGSGPQKDTIDNTPIATSSSSTTHQLFSRSTTFDIVSIRKGPRSLKFWIHKDNLDEIKFLLSSEFKLITDDSLFTKDKGLKNTRSSLNLQEDLANSKGTVSGGQPFKDPSDEFCPETDTLSVWLNNPSHPMFVQTSPLNSTDYDIDSIDNLHVFKANPYSQILVSNASPSPDDNSAKNPILITPVGGLRQFSIASLNKNLVDILFYDVQSKNTSILALDERKKIFEEEWCKADLKGNKQMMQLSLNWVLENNVKPLARISMKKLRYISLDKNEKINFYISLEWDIQVNRTNADGTENPQVDMFPHAVLKINFDVPETEFPASISSLIDSHLVYRVDNFNFSLNNYLISLLIKNNQSTSISDEEMLLFIAPWHTILTDTDIRQLPEVRAKSIPFDRQDRKDSLNPDNALDTATEVNKGILLNKDDIAPSKPGYWNEFDNGSDFGGDDDGFYVYTDHDNDNNFGALDWFFNLLTVRSSAEREDIESNYGGAGLDWLSKDKTNKILHWADNTRDFGSMLKEKILGIHDDTYQDGRVDETRPLVRRTPRGFSFSGTDDAYSADEEDEEEYDSETEAMMRLSNKKRFGTLGLIERENHDKCLSFLYMIMVVFSLVTSSIGTLILSTIFGKSQLPKPQMTAGLLILVVFAIACLLLSIILSGFSICLLLCRYIPAPTWHLSAVWVGTLVATTFFFYGIVTCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG85</accession>
    <name>A0A1E3NG85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_60265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015691.1">
        <property type="nucleotide sequence ID" value="XM_019163421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG85"/>
    <dbReference type="STRING" id="763406.A0A1E3NG85"/>
    <dbReference type="GeneID" id="30180108"/>
    <dbReference type="OrthoDB" id="6512918at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00267">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158:SF2">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="7"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="276" mass="31496" checksum="0095B3AB65141A9C" modified="2017-01-18" version="1">MSDEYVVFTDKLTFQYPDEKRLGLSNIDVQLKKGDKLLLVGPNGAGKSTLLKILAGKKLVRRGRIELFGVNPFDLRSNFMLNDKTIVYLGTEWANNEITKRDIRVDELIRSVGGDVYSKRRDQLVDLLEIDTRWRMNRCSDGERRRVQLCMGLLKPFDLLLLDEVTIDLDVLVRQRLLQFLYDETDRRGCCIIYATHIFDGLGRWADRVVHLSGGEFVEAYDRDNVVFVDTDADAAARRAQPADASVAQLFVAKVASLYPVALQWLSRDHVTRAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG98</accession>
    <name>A0A1E3NG98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_167401</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016288.1">
        <property type="nucleotide sequence ID" value="XM_019161326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG98"/>
    <dbReference type="STRING" id="763406.A0A1E3NG98"/>
    <dbReference type="GeneID" id="30178013"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12291">
        <property type="entry name" value="RRM1_La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="101"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="182"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27674" checksum="DD037DCA990A0385" modified="2017-01-18" version="1">MSTDEQAQQNILKQVEFYFSESNLLNDKFLFTTQNANDGWVPIQTISQFERMKKYRPIETIVNALRKSEELLEVSENGEMVRRKIPLPKNYNEIQLNINKRSIFVEKLPEEATLDDLLKFFTDIAAVNQVRMKKNKEKKFIGSCIVEFKNPQDAEKVLNGENKLKYGEVELDIISKTAYDESKAQKFGERRGNRGNKNKRRGRRDSKDESKEESKEEARKRDASPVREEKSEKERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGA7</accession>
    <name>A0A1E3NGA7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_167437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016293.1">
        <property type="nucleotide sequence ID" value="XM_019161327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGA7"/>
    <dbReference type="GeneID" id="30178014"/>
    <dbReference type="OrthoDB" id="3994852at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="620" mass="70799" checksum="21F6D39300FB44E7" modified="2017-01-18" version="1">MQIKLYGLISCVVTTAFVLAGLLFFTIWLFTLVGKSDSSEGNDYALEHDFSSDVETSSKKNNDEKLLSKESTIKEVYTSKWDLPSKIKEKAMFWKATTSRDSVNDYGYDSLIDIYANETADVSRISGSFGAGFKFAGEVDDYSNMSIGDIHFKKIRSDDDFYEDDRLSESTDGIEDYTTARITSKTENSFQTFSSAILDTASIADSRSFSSTANLVSFQNISLIADEDEDNEDEDDEDSGYGNKIIESYNNSIGQRVSRSDGDMLSRSGSLKNKESEISDSKIHTINMSKIGPTYLYVYLNMIYGNWDEFEYQSVLSGNDAVSDKLNEKIIKKILKTLNKRAIDDFRKEKMLEKLEILRFAFITETYHELILKSVMNNVKFLGEVIKDDKVAENVKRDIFQMFHCSFWYESKTPLTFEFAKQLIKNSIDSLSRMNVDSVGFKDGIRCLHRILVYSNIDDFIGEVFDECFKKVNNLNFEKQNVVWELLKLVICEWMLEADRIEEIKDIYTKLQGNINVLVDEYVEKDNSKYQEFILIWKMLRESSTNGALDLDGGGSGSAVTDENYNPNYRIKKRYNSVSKVKRKLSVTKKRKSRSSNNGSEGSIARQELRDVTNVTKASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGD5</accession>
    <name>A0A1E3NGD5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-B22</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase B22 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_17669</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Mammalian complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016292.1">
        <property type="nucleotide sequence ID" value="XM_019161686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGD5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGD5"/>
    <dbReference type="GeneID" id="30178373"/>
    <dbReference type="OrthoDB" id="13598at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20263">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045292">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033034">
        <property type="entry name" value="NDUFB9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B22 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="17">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ45179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="109" mass="12582" checksum="916BD4DC45CA1A4A" modified="2017-01-18" version="1">MPAPVPFSPQNAKYVASLYRTSLRTARNWINQNAFYREKAAEIRLRFEHNKNISDPAELEKVFAKTEELLAKFKHPDPIIPPKRPGGIMYDRNAPPRYEKGPANFKNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGI8</accession>
    <name>A0A1E3NGI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17684</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016309.1">
        <property type="nucleotide sequence ID" value="XM_019161694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NGI8"/>
    <dbReference type="GeneID" id="30178381"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="763" mass="86054" checksum="4621DDC2DCC4BBEA" modified="2017-01-18" version="1">MTTNPLKKGRYSKLGCAECKRRKIKCDESKPVCWHCQRLEKECVYLPPKWKNKDRSPRTQEGKVTKTTSTDGEYLDINPVKDINNVKARPSKSQRHPRIKTVVMKDNAFQNSADGSSQLDETSKNFLTLSPSLLNAASNNSVMNTIEMIMNPESYKDSTSIMGQSLNDLLTTQLSQTTGQLNNATGLTDPQGSFSFVSNNINTSPFSISSLLSNGESNSPTKTHKILKSSIPHSTLSEFSLPRTHMEYLRLFYEDYSKVIMPFCPLDVQENHSLFPGTGSSEGPSPVPSLSASPSNVTGGIATTVNNLGVGITASIVPITATTVVAPALTTASSIPPIAASAPNNRQSGDTQVKEVSLINPARDIILYYAQKESYVLAAVLACGALTAYRHSKSLTDEEQYCSYLSTCMSLLGQSLNDEATPDKRQKGKMEGMIITTLLLTSYNASSNVVKWRPHLETAGRLLMHSAYYQNNDNTDTIEFGKVERETLAFCRSWFFSIEIIAGMSSNWGGTMNDRDWKVMELWIEEDSDFLRGMRINWKNPSIDNEGVDDFNLMLGYSDKLAIVLGRLCCVLRRARLGEKLKLSEISWFLTQLECKEIKEYYLLSHTGYLNKDFRHEPSLGIPAEAVEKFDDIDLNGDIVYVSWWDICYQAHRMSGIIHTLTKLFMLDRNHFLVMNAIEQTVNLLKFLRTKKKIKSYALMMFQFCVFLVGKWTTEIEHRSLVSKYFRDLHDFGTIAARYSMKKLERLWRNEDGDQEEEDIINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGJ1</accession>
    <name>A0A1E3NGJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016370.1">
        <property type="nucleotide sequence ID" value="XM_019161720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGJ1"/>
    <dbReference type="GeneID" id="30178407"/>
    <dbReference type="OrthoDB" id="10363606at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="731" mass="81691" checksum="0796A33C4AA24624" modified="2017-01-18" version="1">MRDLSYSMNAAWLVVTTLMYATGALGVVLLDKQAKTDIALIIFTIFEWLSAILSTIYLIPFFPSANYLSFIIQALNAFCAAASTAIGIYWIDNIILESVTTKSLYLRQLKVCFITLFTVSLLFKFVIYISAFLDSSVTETNRKEIPNVQDLEANIEVADKESTKFGSVLSDSIASSKFNVVKIKDSLQTLVSKQGNNNLNDYKDNSAKASESRINEPGFDYRSQERLNLKYAELFKLPSTSETIETSDLHNSTPNMLFEQKPLTGASFKQSQILVQNNYIQDIPSTPMMTNLSNHITEEDLEESMESVLVKSPHYQFPQDGSLTSPYKSHHPNYNFILERPALVKNVDEDKNIQALNISEPIYGSNLATKLTSNGKTLHNISLQTWNEKSKLFLQNNDFVTDNIHLPQRASDINHFGSSMSFSENFNAENEALDTHSNNSEYNNCLLEELNREIKENGSHHAKIQMQQSLPNLKLASRHSSQLNRGDSVSKIMGLKPLNQKSRTNSSSLNFAHQKSSSLSAIPMSKSRSHSPLKKFKDFRDSIQLTPRTPKQTRPPSPITSNEFELDLHIANSIRKSPGKKLSSKSLNSKTGSRKISLSSPIRPNTNVSMLDVESYYPTGIGYDLNLSPGEGCVRVLGNAENESPSDKKNYFTHIVDDYIIQNIVKSQRVFSGVSGMSGTDKSTTSNASVPSGYYGEYDKEKWRVIKKVKNGVIEEANKTLDFSCHGLPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGN1</accession>
    <name>A0A1E3NGN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Golgi to ER traffic protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_13036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016428.1">
        <property type="nucleotide sequence ID" value="XM_019160001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGN1"/>
    <dbReference type="GeneID" id="30176688"/>
    <dbReference type="OrthoDB" id="4097053at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028143">
        <property type="entry name" value="Get2/sif1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263:SF1">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08690">
        <property type="entry name" value="GET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35090" checksum="3CC2B4E622FBB3BA" modified="2017-01-18" version="1">MANEPSPAELRKLQRERRAAKMARGSTRLNKILGSPDIKHESEIDDTKENGASSIGKDDIITAASTGKSDHQEQNVRSRSDKNSGDRISVILNQDVDDDPPVSGLDGLETIEPEISLESVDSPLQEFGSDENIEQEMEKLLNKILQNSAHDTSHPHAHGQGITDNTNAMFNGDMASMFPGLKPGMGPTGFGAMPAQPAKSKLDIAKAKVYQSGYAVIRFIIVWVLVSNHIERSSFTSGFVFPQGSRLWLHFLSTEAVLGAIYLLLSYLHIFPSHTIMSFDISGFGYPNSILTSYGLVKSFFTDFCFMIVVLGFYFYFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGS3</accession>
    <name>A0A1E3NGS3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016450.1">
        <property type="nucleotide sequence ID" value="XM_019161747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGS3"/>
    <dbReference type="GeneID" id="30178434"/>
    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="565"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="614"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="907" mass="100170" checksum="9E54A23C39D14B2E" modified="2017-01-18" version="1">MQGQPQHTLSAQQQQQLRLLSLLQQQQQQQESQNSTNSNNNSGNSSGNSNQVQKGNTPANQQIPLSMLLSPQLQNRSLQQQALAALQQSTQQAAQSQQSQAQPQSQAQAQSQLQAQAQSQAQPSNLQHSQQLFQQLQLQQQELLTARRLQAQGQVPGQNQNSVVGAAQNVNQPALTPNTNPNLNLNSVDPNVTNQAQKILSLPQLQQFQQQQQQANTSTPPSLSQQEDSNSLQLLQRYQYQMMVREVWKDNLESELVVIRQLAERYNHIFLSTEFAGIIARPIGSFRSTKDYHYQTMRSNADLLNLIQIGITLCDKDGKRPEDVPSTWQFNFTFDLEKEMFNKESIELLTNTGVNFDKLKKSGIDRFEFAQSLIDSGLCLLDNVTWVSFHAGYDFGFLISLMINKEMSSSETEFDNIMVKYFSNFYDIKLISSLNSIGSYKNGVTLESLGDDLGFNRLLNMSNLTQVGGQSLLTYLCFWELKRLSNLAEFEELKNQIFGLNDDGTFKKHALSNNLVGAATANQSSSLDGQGQAITMDGELNHDSTQSQQQPGQPMLQPLQLLFQQQQQHNSTNTPTSTATVNTTTSNGNQATESTSNANVNQVPHLQAQHLLSRNSNSQASQNQGQNDLQRQAAKQGQLPSANTGVNPNQQYFSNMNNMNVNNINNMNNLNNINMSNMAGGGMNMNGINSNGINGPNNINSGINGMHINGMNMANMSGMNGMNGMSGINGINGMNGINNVNGMNNMNNMSNMNSMNNMNTMNTLNALNGMNGMNNMNNMNNMSNMNNMNNMNNMNNMNNMDNMSNMSGINSMNGMNNMNGMNNMNNMNMNGMNNLNNMNMNIAGVNGMNNMGSMNNMNNMNNMSNMNNMNSMVNVQNGITGANNGQPGGLNGDAKSMVSPRMMFVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGY1</accession>
    <name>A0A1E3NGY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_35910</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016528.1">
        <property type="nucleotide sequence ID" value="XM_019162973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGY1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGY1"/>
    <dbReference type="GeneID" id="30179660"/>
    <dbReference type="OrthoDB" id="18472at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21776">
        <property type="entry name" value="MobB_Sid2p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05600">
        <property type="entry name" value="STKc_Sid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000319">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000109">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF417">
        <property type="entry name" value="CELL CYCLE PROTEIN KINASE DBF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="183"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="484"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="54"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1042" mass="120576" checksum="AC2E1B3F7F7946F5" modified="2017-01-18" version="1">MALMNGIFSRPQKDDSQYVDDLSRDVESLNFGAPSTPKKGYADGSFMQICSPDRSPQYQQQNAQSSPMNYGINNKENTPSSSPSKLQMRSNNLFQTSRLDRAFYTKANSPKTKRLVTVCQMYFLDYYCDMFDYVISRQQRIQQVESSLSTLPQEEKSLQWRNYIGRERAFLRKRRIKPKHKDFDIITQVGQGGYGQVFLARKKDTKEICALKVLNKKLLSRTDETRHVLTERDILTNTRSEWLVKLYYAFQDAESLYMAMEFVPGGDFRTLLNNAGYLNPHHARFYISEMFAAVNALHQLGYTHRDLKPENFLIDAKGHIKLTDFGLAAGSVSTDRIESMKLRLNQVKDLEYKPKERSVSERQQLYRSLRAKDIHYAHSIVGSPDYMALEVLEGKPYDYTVDYWSLGCMLFETIVGYTPFSGSSSDETYGNLKRWKHVLRRPRYDDGRYVFSDRTWQFITRLIASPNDRLRSFKQVIEMPYFSDIHNWESLRETVPPFIPQLDNDEDAGYFDDFENEADMAKYKDVIAKRAHDERIAQNSVRADQKNFVGFTFKHKGNPGINPNNILAPIILNGRSDDRNGYGHVFSDKETNSDILEPQIPIYFSEGEKECLVPKNLSENFLYLGKIGTYKLVSGLKVGYVYGSLENHTAEQIADKFKGKEVDIMLTYYWPEAIAKEENLVLCGNKKLDSLISIAKPRYWFASGTPKGRFFEREAYSIDGRTTRFISLATMNEGRWWYAFKISVDPADDSVVKTGSPPKLILDDNVVRVNKRPYNEDGPVLKKISREKLDTASVTPAECFLCLANPNFELHMVISVANLSYLTISKGPLTLKKPLGFSGHGMIVPIEHYSTLREYVQDKEGETKVENSELVSEITRLQVSLVKMFRNLGDFAVVFWEISRKRSVHCHLQFVPVPNKLAKFFERTLKNQIEYDKRLYPEPLRYHKFTEDLSDKDKLSQTINSENYVLFTLHEDTGVTKYLIALGTDDDKYFDAQFPRKVMAVLLNLKNRIRWDKCTETKEEESIQKDKFQQAYRPYDIMLSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHC5</accession>
    <name>A0A1E3NHC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_19600</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016609.1">
        <property type="nucleotide sequence ID" value="XM_019162130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHC5"/>
    <dbReference type="STRING" id="763406.A0A1E3NHC5"/>
    <dbReference type="GeneID" id="30178817"/>
    <dbReference type="OrthoDB" id="2039at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="176"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="249" mass="28344" checksum="D9EF013A05DD2061" modified="2017-01-18" version="1" fragment="single">TESMPNEKQVITVGVLALQGSYHEHIAHLTSLFQELSDDERYCSKFEFRSREVKKVEDLHTLDGLILPGGESTTMSILLQRNGLLETLKKVIQVEKLPCWGSCAGLILLCNEITNTSVDMSGRNVEKSMKYRSIGGLDVRVDRNSFGRQLDSFSENFILEDFDGELHNAKFTCVFIRAPIIREVLSPKTCYLKEDPHSDFENKIVRLGLNEKNSMIVAVKQGNLLGTSFHPELVSGDYRMHKWFIDRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHI9</accession>
    <name>A0A1E3NHI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_78083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016704.1">
        <property type="nucleotide sequence ID" value="XM_019164959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHI9"/>
    <dbReference type="GeneID" id="30181646"/>
    <dbReference type="OrthoDB" id="10546569at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="930"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1137"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="762"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="786"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="807"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="853"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="938"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="966"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1137"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1181" mass="133292" checksum="2490945899D3CDAB" modified="2017-01-18" version="1">MTGGTVPLLKQPMVLLVDSDPDVIDLTSSSYDESENDETFTTPEKPVDRKREVMVSEGSHVVEHGDAHNSRISVPVDRATETALRKIGDTRVVSSSGDNQSILSTKDRQKRLDTPGGDIAELDKYFEGKVRSIGVDEENRLPVEHVLNRRTGSDQVGLVEQTSPDEINLSVPQSTQQIDNSSSLLTSTSSDSKKDNSTFISGIYSSISKVRNLFLPNNLFTQDKINEEEKEEEEEEEGEEEEEGEEEEEASAVVTRYHQINDSEASKHSQISSQDFKLPHLTSQSTRNNTLNTSSFEVNTISSQLPNQTYYMEKSQQPQPQLASLSLVVPDSTTSLSSSSSQSQSQHSQLTSEQRKTGSTSNDMHDSVSSTKTLLPLNNEPLRSKNPLLQFSQLSGSLLQMRIDKNPQRKKAKSRKLKKVIGSTDEEDEESIAKREERTEEKIATIPSNGKRISSFGLQPISTDKVGKITGSKTFISEEIGLDDTMKIASSGGLFDDDDRHHRQKEVENTCRERVTRACIQTEEETSDRRRLSGSAKHISTIELELSASENEIEESDSLEVLEVNQYLNALEEQSDSNIENMPGDFYDTAANDVVDNSKEKADAYSIQKSDTPKLEESQATQSNVINEMLNFYEEEFQNQQLEDIHFNNDFRENTKNSADYSNLFVPEDESYDSNLIGDSSEQYKSNDKILKEGELRDIGNIAEDDEDDEDEVLIVKKNRKKQISHRLMSLARQKRKKKLSEYNPYSDSDSGSERDESTSDLSSGYISSPNDNKNSRDFIVNDNSVEFESDSDSGDELAKNELLSKLQERSERESKTEKKRKKIESEMESSDDEQQQRKQQRRKPPLQESMRIRGKRARTRSSRLKEKRIEGKDVIVLDSDSAESLGNAEIEPDDHGQMSVVKMEHPFLGDSQMALLEVKSNIEQAFFEKADIRDLEPEQLEPEQLEPEQLEPEQLEPEQLEPEQPEPKEIEPKQLEHKQLELKETEPKEIDPKVIEQGSVPEDQKSEGAEQIDTVPKEFGLEEPKVKEIESEKAEVKEADINKVIGSTKLQFSDAVKHIKKSSGQKTEKQVNETTPANHPASPTDCSSCTRQKNSDLIESCDRQEICSNCKRQGVACVYHPEKGTTKVDKTLKKFARKQSAKKKKKAPKSTDNSLLEKLNERLRVKHGKKKDKRNGQTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI08</accession>
    <name>A0A1E3NI08_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">High osmolarity signaling protein SHO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">High osmolarity signaling protein sho1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">Osmosensor SHO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_73287</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016898.1">
        <property type="nucleotide sequence ID" value="XM_019164437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI08"/>
    <dbReference type="STRING" id="763406.A0A1E3NI08"/>
    <dbReference type="GeneID" id="30181124"/>
    <dbReference type="OrthoDB" id="5983572at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11855">
        <property type="entry name" value="SH3_Sho1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035522">
        <property type="entry name" value="Sho1_SH3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="296"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="356" mass="39579" checksum="2C9F9471C8DEE197" modified="2017-01-18" version="1">MAMPQIPTPGIPFNRNSDNGVGREPDRASVPEKRFSSLSFNVIIVATITVSAISWIIAFAGSIAASQSIKYFPKFTWWGLVFELFVIIAIPVLYFFNLIFLYRQFLVAMIGIAFVYTSNSTNNLIYNSDSSCSAAGTGFILLSISNLMWLFYLGSDPNAPVIASINRYGGPNQMATRKRISSINNRGNSYSYNPGTQSTTRLNFDHNQYNSQYDDSDKNNGDVYSNMEDAHELAGFENPASTTQIDTNRMSNIEFNDSAFRASGIMPPHALINEDSDNPEGTNFTGISSASAYMEDYPILVRGLYDYMASPDDVNELSFAKGELFRVKNTNGNWWQGKNKRGEIGMCPSNYLEIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIE8</accession>
    <name>A0A1E3NIE8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_34735</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017020.1">
        <property type="nucleotide sequence ID" value="XM_019162903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIE8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIE8"/>
    <dbReference type="GeneID" id="30179590"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF171">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="24"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="252"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="530"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="860" mass="98192" checksum="4E507945A1280234" modified="2017-01-18" version="1">MCSAVSNRRFEDTSLHEQLPRDVKPVHYNIFCHNIDYEKNLFDGTATIDFEVFSKTKSIILNQKFLSFNKVVLKTSKGEEEVEEVIKDDANETVEFKLSSEIDAALKSVQVVVDYSGIIRTDMAGFYTSNYKNEKGETKYILTTQFESTDARSAFPCFDEPNCKASFTISIEVPKDLQVLSNMPLDKEVVADSDKENKLFTFCQTPKMSTYLVAWAIGQFEYVECTTTRDYNGSKLPIRVYTIPGQSHTGKYALETAQSAVDYLSSIFEIDYPLPKLDLLAVPQFGANAMENWGLVMFRSTALLYHPEKSSALYKQKVSYVVSHEIAHSWFGNYCTMNWWSDLWLNESFATYVGWLCVDNMHPEWDVFTDFISGSMQTALDLDSLKNSHPIEVQVYHSNEIDEIFDAISYLKGGSVVRMVAESIGVELFLKGVSNYLKKYSFGNAKSDDLWDSVSEVSGKNITELVEPWIKAIGYPALKVVKNKDGKTINITQQRFLVEGATKVDDSITWWIPNIDNMLTKEKVIDAQNFLKLNKDTAGFYRVFYDDELFENIVQNLGKLSDNDKIGLIADTSAGARAGIIKTSQFLKLVSQMKSETHVSVWNEIIERLNILKNAFFSDEEISEKLILFSRDLYKNKFEELIKSANLDFNGQRLASALFEQAGISQFNVAVTKALSIYQSKDIKPHFKQAVYRILLANADTCNDDVFNSVLAEVREPSTIDGREVALRSLGSINKVDEYLPSILSLFFDGSVPEMDYQFLTIPLASNFKTKVFFWNYFKDNYPKFRNDVSMWTLDRVVKGFLPKLVSKDLVQDVELFFENKDNSGYEKGLRQSLDSMRNDVRWRERARGDLLAWFQSNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIF6</accession>
    <name>A0A1E3NIF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transfer RNA methyltransferase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TRM8</name>
        <name evidence="13" type="ORF">PICMEDRAFT_17174</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a complex with TRM82.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="11"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017035.1">
        <property type="nucleotide sequence ID" value="XM_019161496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIF6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIF6"/>
    <dbReference type="GeneID" id="30178183"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000060">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="133"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="168"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="292" mass="33992" checksum="D88BFB65AEE8AC20" modified="2017-01-18" version="1">MSTEATPVPVKEGSPSESSPDPTVPSKRKQYRQAKQEERQTKQVKIAEPTSDMKETKPLPKKRYYRQRAHSNPFSDHKLDYPISPEEMDWSKLYPTDENEDKQVTIADIGCGYGGLLVALAEHFPERRILGMEIRVQVTEYVEERIRALRESHKDDAKFNNCAVIRANSMKFLPNFFRRGQLDKMFFCFPDPHFKQRKHKARIITNTLLSEYAYVLKEGGILYTITDVFDLHEWMKKHLDEHPMFERLSPEQEQSDVCVDIMTNATEEGKKVSRNNGSKYIACYTRLPNPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIJ1</accession>
    <name>A0A1E3NIJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA adenine N(6)-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017062.1">
        <property type="nucleotide sequence ID" value="XM_019161506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIJ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NIJ1"/>
    <dbReference type="GeneID" id="30178193"/>
    <dbReference type="OrthoDB" id="16079at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="42785" checksum="19B1159FA80A3B0D" modified="2017-01-18" version="1">MQKFYSSQKSVKGALEALADKKIKGIRFNYDKSVYAEIFEKVNMKRTYMDRNGNPTGRLNIIDYNTRLSPMTYHLNTVFEPEHHLIYPDGQAAFKFWKNMEIEELEMKNIHVLQTSVASTVKANLLTKPLQEKLINPSFLPTLSDEEEQKLKEEHIEPVNDTILFIGDFMSSTHAGALRTCIYYNEVRTSMFRYGGVKFLAWTTPNEVLKYVGPLGSIHRRTNAMMANLYSDIRIIACSEKLSNPKAMRLVGEMDTVKLPFNELDGEVCLLELQSNHNKYDIKFHDELHLIIHKLFCTPGALLDEKLHVLGPGAEEYLRSNVPQEVLHKKISMITNQEFVDLSEIYFYWPFKPNTSLETYGNQDSFFDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIP2</accession>
    <name>A0A1E3NIP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017122.1">
        <property type="nucleotide sequence ID" value="XM_019161524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIP2"/>
    <dbReference type="GeneID" id="30178211"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="53" mass="5588" checksum="94339B2C363F6992" modified="2017-01-18" version="1">MTCRPRPVTPPVTAPGFLDPLPGRWRGARGPTQAQGKSGRMSAAAEKEPEGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIZ8</accession>
    <name>A0A1E3NIZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_12543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017222.1">
        <property type="nucleotide sequence ID" value="XM_019159944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIZ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIZ8"/>
    <dbReference type="GeneID" id="30176631"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="116"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="441" mass="49286" checksum="61B3C3059B7A00C5" modified="2017-01-18" version="1">MSTTTTTTISTISKTSSISAASSTGVTGGGSTLRCVAAGGPTRSAEAVDTTGRRFRAPDFSINDVLKAIPKECFRSSLAKSGGYVLRDVLCMVVLACVAHTAIPLVGSAALRGVCWAVYVNLLALPMTGLWILAHECGHQAFSDYGWVNDAVGWVLHSYCLNPYFSWKFSHRKHHKNNGHLRKDMAYVPRTLDEWKHKRGVVSLAELAEDSPLVSIATLLVQQTVGFQAYLLFDATGQPHPQLAASTSWWRRHFGASHFNPHALIFDKSNFWYIVASDAGILLQLAVLRLWVRRLGAFSCLVDWFLPYLLVNHWVVFITYLQHTDMSLPRYTDAQWTFPRGAGATIDRDFGFVGWFFFHDLIETHVLHHYCGRIPFYNARKATEAIKPVLGSHYRQSDTNMFKMLWQALRWCEFVQGDNGVMMFRNHNGNGVPPVDTPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJA2</accession>
    <name>A0A1E3NJA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72309</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017335.1">
        <property type="nucleotide sequence ID" value="XM_019164155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJA2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJA2"/>
    <dbReference type="GeneID" id="30180842"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000414">
        <property type="entry name" value="Zinc finger protein 398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF58">
        <property type="entry name" value="TRANSCRIPTION FACTOR (TFIIIA), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="511"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="547"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="734" mass="81940" checksum="3782463BC56D38DC" modified="2017-01-18" version="1">MNMDWHDSINPFFTTSSGSSSYGNGNSSRSGGSSDRIMENPLKIDTNSSFFPSSIFKPIPLPDDILGFDPLPQSQVHDVQQRQVPPLPPAEGFNASFDLEKYLKSDLDEELDAENLDNDFLDANLQFNMNFPNMDSKETDIEKVLLDLGISEVPKQNVSPEQPPVTETPHAPGHKRKISGSGIFGFVGVGENTQLSIPGIEPVTFINKKPQYKDVTNFDNLGPSYINGNRNSTATDAATISKDNYAKVNSSSANLDNFIPNVSLTNLQAPITPVKQVQNREKPDYYVSSGNPVSYKFPPSPSKGTFEPTENANREYPPNKSYSAQELQNLRQLSKSKSQQQMRQPTIVSTNHHHPKSPLPTPLPTSPLNSVAMSSPLKEMFQTPKTGSPKKVLPLSQLQAQSQFQVRLNKQLRDPDDTNIIDDDKDKTISAMNTPLKLKLSAEMLQTPTKSKFTNLGWDPTMGPKKPHMFTQRILKSPKRSPIRKKPTITSTLATGTLDKYFEGPTAEGKFVCRFFDQEIQGVCNREFGRISNARAHIQTHLSDRPFVCEECGKAFVRNHDLRRHQKGHSVASNICPCGKRFPRSDALKRHRFRNICVGGIPRNNGVFKPCTHGHSHSHASDKMTTMVQINSKNKNNQKITEKLLGDINKEGQPIIRETIFTSQQEQPLSQTSKFSHLSQPVSLHQSQYTDRPLLPNQSLFNTSSSKDIDLFDFNEIASVDGNFSFNMDDSLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJH6</accession>
    <name>A0A1E3NJH6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017407.1">
        <property type="nucleotide sequence ID" value="XM_019164182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJH6"/>
    <dbReference type="GeneID" id="30180869"/>
    <dbReference type="OrthoDB" id="3997394at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="318" mass="34055" checksum="2E3CD0896812A397" modified="2017-01-18" version="1">MLRLVYDYPTVESSEPLETYARTSPHRYSSIAAADKYLLRYASNTWTVRFPLCVVLAQTQTVRLPVVFDTRGGGGGPLSVEIPGQSLSAVGLVAVQECSETASVTLEAGCEVYFLAESVLVRLPQTERLSTLYPQLPRDGLAFCFSYTDILGHRIQDNLTRLGLSVQGCEMTLSPLQRFPPQPLVAGVFQRYADLTPLQALQLVDSAIVDGRELVRKMTEYTRSAGSAGTSAGTGAGTEDEVLLFQSGQGDDDGGGYAESYTEDDAVGMHVEMVGGFKAAGVKRARRRSDEAVAADNDVAAAAAAAAAKHKRLYSAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM9</accession>
    <name>A0A1E3NJM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_18623</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017465.1">
        <property type="nucleotide sequence ID" value="XM_019162080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM9"/>
    <dbReference type="GeneID" id="30178767"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120271">
        <property type="term" value="P:negative regulation of nuclear mRNA surveillance of meiosis-specific transcripts"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000257">
        <property type="entry name" value="CCR4-NOT core complex subunit Not4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000006">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="8"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="112"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="16">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="27228" checksum="BC37F818BF89B398" modified="2017-01-18" version="1" fragment="single">SEDEDDFCPLCVEEMDITDKNFKPCPCGYQICQFCYNNIRTNPELNGKCPACRRPYEDKNIEYRNITAEEWKLQKAKIEKRKKEKKIIEKEKKDAEQAKRHHLAGMRVIQKNLVYVVGLNPPCSYDDLIPVLKSEKYFGQYGKINKIVINKRAPNPNNPHHLSSSPGYGVYVTFARKEDATKCIAAVDGSISDGKLLRAAHGTTKYCSSYLRGQHCPNPNCMFLHEPGEEADSYSRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJN1</accession>
    <name>A0A1E3NJN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017452.1">
        <property type="nucleotide sequence ID" value="XM_019161133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJN1"/>
    <dbReference type="GeneID" id="30177820"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF9">
        <property type="entry name" value="P450, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="60983" checksum="2A7BEBFA55C140D8" modified="2017-01-18" version="1">MLQTSVFGNLSTLQLGGVVLVLGCFCLAVWLYNFTIKPSQPKIDGLPEVKTNSPFFGILTTLGEDHPTNLQKLSLEKGAPVFQCLMGTQRVVFVNTYDAANEWFIKNQTALIDRPLFYTFHQLVSTSQGLTIGTSPWNETCKKRRLNVQRYMTTPAIKERAGLIDLEGFSFLRDFYRDSQGKYIYPYKYTQRLALNFTAMLSYATRFDKIETPLLNEILEMVKLISSFRSTNNNLQDFVPFVRLLPENERKRQAVEASEKRNIWLGKLTEEAISHKDTRKSIVGDFANTDGKARLNIDDIKSICVGLVSGGFETIGSTASLILLQLVSPNGAKWQQRIHDELVEIYGSVESAYENVLCEEKCEFGVSFIRELLRMYAVIPLIPARKTTKPFQWNDTVIPEGVTVILNAQAANHDENHFGPTVHEFDPERFMTDKSVDVPPYHFTFGAGSRACTAINLSNRILYVILTRVCLLFKVHGDPSKTPDFDYLNYAADRTAQTYWPKEFGIQLEARDEVILDACMSASKAKCTEELGYSIEYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJP1</accession>
    <name>A0A1E3NJP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">mRNA 3'-end-processing protein RNA14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_23910</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Nucleus and/or cytoplasm.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017444.1">
        <property type="nucleotide sequence ID" value="XM_019162329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJP1"/>
    <dbReference type="GeneID" id="30179016"/>
    <dbReference type="OrthoDB" id="26282at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045243">
        <property type="entry name" value="Rna14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="5">
        <location>
            <begin position="5"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="439" mass="52826" checksum="F1F2AF6C8E4A2F42" modified="2017-01-18" version="1" fragment="single">DEFTHEEWVQLLKKTLEGGEEENITKLFEQYLTQYVNDGEQWVEYIKWMMKGDDVEKMDKKRIESSFFKVLTKVYNVKLWRLYLHYVELINPITPDNGEKARNIVLKAYNFAIETVGHDFFSSNEIWGDYLKYLYVWQAVNPNEASTREDLIRKALKNMISYPSLNLEENWKIFTKFETDLNLNKSRKVISDCKNDYLKLKGLNNEVIGLTKSIKKVDERKHSLRQIRRWNSWINWEKENKLKLTEEEANKRINYVYNLSIQYCYLMPEAWFNFSNYLVVERNNAKLGLEVLKDGIKVNPYSLILRYQLSNHYEGANDLEKIKETWLDLIRRVENSAKKDDRLITYCYSILMRVVKRISNIKEIRMIFKLARQFKGITWNIFYQYAMIEYYNNEIKIANRTFALGMQYFSTEISFVQKYLQFLIDIKDMTNFKKTIELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJV2</accession>
    <name>A0A1E3NJV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16310</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017538.1">
        <property type="nucleotide sequence ID" value="XM_019161160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJV2"/>
    <dbReference type="GeneID" id="30177847"/>
    <dbReference type="OrthoDB" id="25767at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02569">
        <property type="entry name" value="PseudoU_synth_ScPus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000020">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041707">
        <property type="entry name" value="Pus3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="5">
        <location>
            <begin position="218"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="437" mass="50028" checksum="9A10C5B3CE55B4C2" modified="2017-01-18" version="1">MSFQNGGEGGQLDYTNWSKKQLLKRVLELEGRPIPPLQNSFKVVDNQTSDNVLPKKHKKQKHFDFSKYSTRKIALKFAYLGWDYQGLALQGEETNLPTIEAKIMEALYKVKLIGSLDQGQCEFSRCGRTDKGVSAMNQVISLKVRSKLSEEELKDNANDTKEIDYIKTINQSLPEDIRIHSICLRPPPDFDARFSCTYRHYKYIFNGQGLDIEAMNQAAGYFLGENDFRNFCKIDASKQIHNFKRTILCSQIDQLPGNENLYVFNLKGTAFLWHQVRCMIAVLLTVAQRFEDPIIVRELLNVEIYPSRPVYKMAHDIPLVLYDCGFDEALVHWSSGPKRSFNVNKAVDGLWNDYKVKSTIVDFMRKFTEEKDADGRSGKIHVNLGDGIGQAMNSYTKYGSRTRLETADDINAKWKGKKSTSKLSQKRNHSEIDSESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJZ5</accession>
    <name>A0A1E3NJZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_11462</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017577.1">
        <property type="nucleotide sequence ID" value="XM_019159800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJZ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NJZ5"/>
    <dbReference type="GeneID" id="30176487"/>
    <dbReference type="OrthoDB" id="432645at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17661" checksum="ACF28C61ED7B7FF9" modified="2017-01-18" version="1">MFRALRQFSTSRASLGPKMQVYEPLPRLRNGQCLMEYVKQAQYEKFDKTGAKRKALSLANKERIRADDIVTVVYKNQKPVTGQVLMVKRSGLNSTVLLRNKITGLGVEMNIPVYHPNIIRLDIVRRPVAYKPRNRHYYIRNSRLDVGEIGASGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK54</accession>
    <name>A0A1E3NK54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_21606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017614.1">
        <property type="nucleotide sequence ID" value="XM_019162215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK54"/>
    <dbReference type="STRING" id="763406.A0A1E3NK54"/>
    <dbReference type="GeneID" id="30178902"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="1">
        <location>
            <begin position="89"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="307" mass="36319" checksum="06AFF29470FEF336" modified="2017-01-18" version="1" fragment="single">FFFSGCLSIVFSQFLFLTLYFGDKNGIQQLLDYTKRNFVILLTFCTSMFSSPSKIILSFKSDELRQRYITVRPDQKGNNRYELHFDPSAVIISNHQIYSDWFFLWFIAYLNHCSDHFFIVMKKSLEKLPVLGYGMTNYHFVFLSRNWQLDKSYMVKQFQTIKQLSTKFWMLIFPEGTNMSHNNRNKSHKFAEKIGLPKQDCVLLPRVKGLYVACKELAPSTTKIIDFTIGYSEHGKDEMAQDIFTLWKIYILGESPRNISILVDDGSHDIVPAEEEEREMQLLNSWITEKWQIKEQDMEYFYKNGQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK65</accession>
    <name>A0A1E3NK65_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017624.1">
        <property type="nucleotide sequence ID" value="XM_019161185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK65"/>
    <dbReference type="STRING" id="763406.A0A1E3NK65"/>
    <dbReference type="GeneID" id="30177872"/>
    <dbReference type="OrthoDB" id="10070927at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22016">
        <property type="entry name" value="HMG-box_NHP10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056513">
        <property type="entry name" value="INO80F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24245">
        <property type="entry name" value="INO80F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="207"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="207"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="405"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="506" mass="56215" checksum="36E2D805D0FB5CA0" modified="2017-01-18" version="1">MFQTASDQSNPGNTVNAGASKDQKERELSNQLKDAKHNKATKQIIPFETEALRLKQKCKDLKQKIADIEAQNQVRAIGVSRAKDSISRLRFEYSLLLESLEKKSDTLGFPDLKSFTPSDIDADSIESLSLEDITKLLGTTPLAISNIQSLLPNALGDLISERHQQYINSSSATEAAPAANGRTRRKRGVANGSTASARKRARDPREPKRPTNAYLFFCDSERDRVKAEWAENHPNEHIDLSRAMTDVWRKMSNDDKKPYFEMYEKDKERYHKAVEEFATIKEQERLAATQPAADASVEPEAETPAGAITTNEITPVDAGHDDEADVSMQEPENTDVTYDEHKSALKNEEEHEEEGVVGGEVEGYNQDNDTSKASIVHENGQSGNGDHPASHNLSDTEEQFHSELHEEEDEDDAGEDDDDEEDDDGDGDGDGDGDGDDDGDEEEEDDGEGDAEEDAHGEQEEDDDEAEDIAHSTDATDGIQEDDERLEIPMLKSEPDDIFFNEDNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKB0</accession>
    <name>A0A1E3NKB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_58854</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017690.1">
        <property type="nucleotide sequence ID" value="XM_019163323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKB0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKB0"/>
    <dbReference type="GeneID" id="30180010"/>
    <dbReference type="OrthoDB" id="2595934at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR OF PROTEASES PRTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="113"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="809" mass="91541" checksum="625F7C8751C5BDF8" modified="2017-01-18" version="1">MTANTQRPTEHDDEHQNKQQAAYSPNESSESSKNHDADEMDESAKNLTDKKGKGKRKYNFQRASVACEQCRKAKTRCNYINNGSNCFRCENLSLKCSLNEIKLEPTLSTFQKSANLKTRDEAEESRSLKRQRKAKLTAAGAATASPKIKQENIPASTSTIDLASLKSKYIDTSPVISSTSSQTNSSSHNSMLLVINDKIDRLASTLKNLMDQKETLSSIRKTGPSGTVPNLQHPTLDTSVNPYINSLPSINHQSPVQIGDSIASPGIPPFLYPPRVNFPFVGPPAPAGNFAPNNNVSPILRPAITAPLARPYFRNINIMNPTHYPPVVTNNSNNSAIAAGLNMNINNITHDNNSINNNNANNNSLMAPNSATTSHASTVTNMVHENDEFFFLNAPYLDMSNIACNLGLPFTKEIGFDITRDVSLLSIQERYDLINRKLLDYETCYRLIEAGLFHYGKWITYVEADYKVWLDSIRLTSPLLFCVLILFGLRHQRPPNLDKNLELDVLQSIHQLLSLSVYEVPQSKEFIQASILLAHFSPSLSYKHIYFDSWWVSSYGLIHFMTREMNMNLLVKSVKSPEKIHQYRLWNHLTVAHLVNCILSGRPCIIDEIRLDQCRDILVLSEANSFDAIVVAKISMILTLYNSLQFPENVDVSLKELESTYSDWKYLTDNVALGSVVTGFYHFGRAMILRRYLLKNLYLKGQPKYTQRSREFFDNVLELIELGKAKEELLDTSDLFKQAFFLCVFMLLQFKQLDLFKEPNDNFIIAEASNKYLEILSLAEKKSYCFNGYFNHYADLILRLKNLVLPTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKE2</accession>
    <name>A0A1E3NKE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_12465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017141.1">
        <property type="nucleotide sequence ID" value="XM_019159930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKE2"/>
    <dbReference type="STRING" id="763406.A0A1E3NKE2"/>
    <dbReference type="GeneID" id="30176617"/>
    <dbReference type="OrthoDB" id="10045710at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="885" mass="97727" checksum="BB931A61F88459D6" modified="2017-01-18" version="1">MTMSHYTDAAAPGGGNAAQSPGSQAAMAAAAMSFSMDQRKNDSSAGASTAATPVYRRRKQQQAQSQSQAQSQVQPQGQRKQPYTPRLATDQPSFPQSEMQVDASSRSPTNRHYRQPPERKIASPQTPLPASSASSVVASSASTLSPSSQNNKSLWNDLNHSSSTTALAAAASIAKMNPSSLISNTTSPKLVRKPIPTATHSMIHSTLRKSPQQTSTASIPRYHSSASTTSVNTREDTRPQSNNGRNLPRKPPPPSRDPTNENLNSSTDIASTHPSSTTTTTPAAAAAANKSFDMDKMASTSSPFQFPLQHNNSYAVQSNVSIPYPHSKNSSPNSTPQPAQNGFHSSSELYPAPELHRRSLSKSQKLTNFLTPKTLKDEIKSQRLYNHQLSNSTTTLPSQIQQLSVNEPNSLTSDLLSQPMTAPTMYASPNGSTDYFSHQQLQPEDFYSNPNMSASKVMNNNNNSSSNIYQQATAVPPQQLVFRTTLRNGDKKGGFGRSKKSKTEFNEDKPWKNHERGNLNTVTADEKKRYEGVFAANKGLYINLDLRLTDQFKLKTVKVGDSVTSEPERTLADFVNTYSTPSERIHGLVVRDIWQRSRLDQETLKKMWSLVLDDRKRRWIKSISNGDTEWLLVEDADVDADVDVDVDDVATVNNDVSQIKEHDTARDADGNPIFKEGFTLDQAVDTKNDTPIEQPPVQGVIPRISITVNPEDDHEENTEYNGSDVSIKTKETQENEIDDILESPEQEDDQNSDMKSDRQVEVQTIAPQDQHRKRSDGLCDLLDTDRNLFDDGTLTCDEFIVGMWLVDQCLYGRKIPKIVPITVWETIGVDWTVSSYGAYGNYPYHFYHPHVPGINGVSDIVGMGIKKGKKGGKNRRHVFKKVIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKE8</accession>
    <name>A0A1E3NKE8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_20668</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017735.1">
        <property type="nucleotide sequence ID" value="XM_019162169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKE8"/>
    <dbReference type="GeneID" id="30178856"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="7">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="131" mass="14767" checksum="CB3E55B60D9C3717" modified="2017-01-18" version="1" fragment="single">LANERTILAYIRTSLNMVICGLLLLQLSKYVVVAPINGMRNHAALDDTQQQIYDDVIRILDCVYRFSKPLGGLLLAISLATLVFGGLRYMRMFQLLFSDHDVFESGLLVNLVIFFSVIASVVLAFVYTYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW1</accession>
    <name>A0A1E3NKW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Choline kinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_52637</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017890.1">
        <property type="nucleotide sequence ID" value="XM_019163165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKW1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKW1"/>
    <dbReference type="GeneID" id="30179852"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007521">
        <property type="entry name" value="Choline_kin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04428">
        <property type="entry name" value="Choline_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Choline kinase N-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="599" mass="68390" checksum="B56C6089AD25C54A" modified="2017-01-18" version="1">MDRPAYTRKRSSNPASPLLVARRATAASGASSSSSGAIPMRPRSNSRNRSRSRTRRASGASSTTTDTGPSLLSPATAAHEVAHHHPHSHHSHTSVSRAGSVVSLDNVLNVNLDNSLPLDYFKQDILSVLKALRIPKWRKISKSNYKAIHLDRISGAMTNCVYKMTYHSYYPLLLRIYGDVENIIDRNSELLTLIRLSRKNIGPKLLGCFNNGRFEEFLNNSITLNKSQLRDPKISRMIARRMKEFHYGVNLNYDEYNSGPKAWLLIERWISVVDKLIENSSAEDQKNVFILNWADSKKLIISYRDYLYSTYGNVENLKESLRFCHNDTQYGNLLFYSNEITSLSLSNQMDQLSLKHHPTAMSLSDSINKDTPLPIVTDTNFKYDTRLTVIDFEYAGQNPPAYDITNHFSEWMYDYHDPQFNYKTDESKYPSVEERINYLNSYAQYLADLPPSVTHLYNETIYWRSTSSIFWALWAIISRGSIDTASNEHINNNNNNNAGKSNSLDFKTPKFEIGPNGEQYKIIVDEIETMNENESGSDDEFLKPTLDDEFDHQKYALGKIGIFIGDMIQFGLLKKEAIDTDRLKDIKFLDTELLPLTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL10</accession>
    <name>A0A1E3NL10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NAD+ kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16646</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017937.1">
        <property type="nucleotide sequence ID" value="XM_019161290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL10"/>
    <dbReference type="STRING" id="763406.A0A1E3NL10"/>
    <dbReference type="GeneID" id="30177977"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000009">
        <property type="entry name" value="Poly(P)/ATP NAD kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="463"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="56042" checksum="FEDD441DC0C30CEE" modified="2017-01-18" version="1">MADNTRVPRFMRRSSSTVQKVPILHEALTKEAQGGLSSSLSSASSSGTSISIGNGSGNAGGHGNVVTGMNAITNGNGSPGYNLQKKMSYRVRDELESNTRDAESDSTLFNTLSDTQGLKSVRSHMDLAKTAHSVRALAKRLNRATVHLNLSSVIIITKARDNSLVYLTKEMTEWLLLREPSLVVYVDKNLRTSHRFDAVGLVKDIPHSAERLRFWDKAMVREGQVAFDFVITLGGDGTVLHASTLFQSAVPPIIPFSLGSLGFLTCFEFEKFREVLTQAITKGVKTDLRMRFTCRVHKCNGDIVCEQQVLNELTVDRGPSPYVTMLELYGDDSLLTVAQADGLIIATPTGSTAYSLSAGGSLVHPEVSCMCITPICPHTLSFRPIMVPDSIHLKLKVPLRSRSTAWAAFDGRTRVQLDVGDYVTVCASRYPFPTVKNEKDQYFESVSRVLNWNNRKEQQSFKHLLSDKNKKRCDEDDGDRNGDDSANEAFDIDYSDSNSDSDSDSDHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL76</accession>
    <name>A0A1E3NL76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16700</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018005.1">
        <property type="nucleotide sequence ID" value="XM_019161312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL76"/>
    <dbReference type="STRING" id="763406.A0A1E3NL76"/>
    <dbReference type="GeneID" id="30177999"/>
    <dbReference type="OrthoDB" id="5298304at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901604">
        <property type="term" value="F:dethiobiotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015295">
        <property type="term" value="F:solute:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905135">
        <property type="term" value="P:biotin import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000399">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF33">
        <property type="entry name" value="VITAMIN H TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="59"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="535" mass="60102" checksum="09B17CA9E4432741" modified="2017-01-18" version="1">MQSNVELESSEDCIKDSAGVDELTHGVCEQQDFTEVYPDDYIPTEKEMDSIYRHLDYRIIPACWVLYFLASFGSSAYGNTLTMNYEAGHSLAQSLKLNTHHTSTATALYYVAYIIFDLPMNLIMTRASPQVWLSRIITGVGIVYICYAALKSGSGLIAIRFMTGFADAGTWPGLSYYISLWYPEHRTARRVGYYFTAAQLSASAAGLVAAGFQKMDMDRGLYGWKWYYIVYGAITVACGLSLVWWLPDRPAYLKPNESNMLSFRKVIPDGVMTFFTPTQPLNERERRLHYLDMRYRYKNVRWGLKDVCLILLDIRTWPLVMMYFGVVGTGFGLAVFATTLIRVNHPTLSGIDVSLLYAPIWLFDFAAIIIFTPIADRFKRWRHFIFSFSTLIIIAGLLVTSYAHGPWNRYAGLLIAGFGLGPTVPITMTLATQIMTHRYGDVGSAASAAIISGLGNLGTVVATYALYSGWPADKPRLYRDSNMMLILMLGVSIIAAVCCWFLMEKVDGGVYDKNAKEIDREMDNKSQTDSDSTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLC7</accession>
    <name>A0A1E3NLC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72971</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018065.1">
        <property type="nucleotide sequence ID" value="XM_019164348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLC7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLC7"/>
    <dbReference type="GeneID" id="30181035"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF15">
        <property type="entry name" value="ARYL-ALCOHOL DEHYDROGENASE AAD16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="352" mass="40060" checksum="9570FF5CF2AE9C33" modified="2017-01-18" version="1">MASASVPAEFFTNLGPSGLKISRIIVGCMTFGKKYWSDWVIEDKEKVFAIMKKCYDSGLRTFDTADTYSNGYSEVLLREFMEKYNIQRDKIVILTKLFYPVEPDVPGFSAAARNQFPVYEFVNSQGLSRKHIMDAAKGSNERLGTYADVIQIHRFDPSTPIEETMEALHDIVKLGYTRYIGASTMRTYQFVMMQNVAEKHGWTKFISMQNYYNLLYREEEEEMMEYCKLTNVGVIPWSPNARGVLTRPHGATGETNRTAADAHRMKKLGLDVLGDGDQEILLRVEEVAKKHGVSMAVVSTAYVLSKGYSPIVGFSKPERVDDAVEAFSFYKKLTPEETAYLEEPYTPKKWTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLF5</accession>
    <name>A0A1E3NLF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial peculiar membrane protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018082.1">
        <property type="nucleotide sequence ID" value="XM_019164354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLF5"/>
    <dbReference type="GeneID" id="30181041"/>
    <dbReference type="OrthoDB" id="4044171at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035187">
        <property type="entry name" value="Mpm1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17234">
        <property type="entry name" value="MPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="32458" checksum="46E4D851D7F50FAD" modified="2017-01-18" version="1">MGIYDANKDKVEDQGDVKINSVSAGSPLDSLQDLNGGLNGLHDGLQKTLSLTNDLTLNALGKLRGIGDGWNSLLDAWDPRNDGQIPLLGPGLLSPRVLHKPNEMVSRGIPPPEIYEECTKAEGLSLWDQQGLWHCLFPRSKIPYDYGTGESHAFGVGPSEAKELVSREDVEQDTENKHGLFFKNLEDMLGWQASMKRAVAEQRRRQWSDWEAKEKAKWNVWKSNSSSGGADGSSGDDNGKEVVGRETETRMVTLDNGDFQRTTTQRRVYSDGTSRIWEKTEVVGPDGTIKKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLK0</accession>
    <name>A0A1E3NLK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ47001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018114.1">
        <property type="nucleotide sequence ID" value="XM_019161346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLK0"/>
    <dbReference type="GeneID" id="30178033"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="879" mass="98205" checksum="99081ACDB180D90A" modified="2017-01-18" version="1">MQRKNLSAQHSVIQQQIQQQIHSAETNVSFGETLKSSMRLIRSMNSNVNSIIHSSSSKYSTMDSSIARDRLSRRVNNKSLTQSNPYLDTRIVTSSLAPDVVADTEADLHPIENVEPVASVSVIEDINGSEVHDKQLDNFAGLTRQPQIIEPPTPEVVDIVECFDPPQNALPYLGKAGEVDKNLRLSHLIKLAKARRELVPILNDYYTCSFWNHRALLSDCAMPQSFYKVAYQYFGESINVNHFVELVGVNRLSRVGWRLPCTNLQQSNFVTRYLAAVENYKQQCNTHIPIVKKDCNGRWVTEKSPLYLGADLNEILNSLPSGRIIDEQFPTLGRISSEESATQVRVEIQTGGDGNKLRVFSNSSETRTPKNSSTSKARNIEQKLLKNVDAEAYYIPPPIVKGSDEKENLAGTFNPWIDSTSSSQEEKFCFLSEHVIAKNSMISVKSTSIEHTSAKCDSEDEKLKDSEAQFFTLDGEISTSQSDRISFHTCDLSLSPCGTEVEKMIDEKTDFLFSSVEARLNRIPINERTSMTVIQCVEEEMATRPPRMRSFFYESKANYLKTGSPIGPKFEAVTKQSRGFTSSSDYGLGVDINDFVDKPEICPNALVSETAFGKPISCTASEKNSSNVQSVGKLAHFNTFNSKANRNILNPLAEAKIVELERKCVSSETRAEDETELTTMEAEKEFENCDILNFKCKANSWVSDDKNVIHEVADVLKSKTSWESSSKLDARMINDLEMPQKKTDTNTRSRLVSMSGDAANEIDCEIVDCSNKYAGVVVQVEELENLFKLMLQDKVDQDRLEALFSRESSSARSISKDYLPNIKAASDIHDARVSTANTNSSDGATDILDIIDDYAIEGSCLLNDENHSMNANRLCLKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLR5</accession>
    <name>A0A1E3NLR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_10129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018208.1">
        <property type="nucleotide sequence ID" value="XM_019159633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLR5"/>
    <dbReference type="GeneID" id="30176320"/>
    <dbReference type="OrthoDB" id="1378at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="34388" checksum="718CD0A745B63E08" modified="2017-01-18" version="1">MTPSIGYVHPTLLLSLSDHLNRHTAYTAGSASTLFGLILGRHTKGESVSLLYSFEIPEIECTSREEFERHLNLLGEVYGKGYLELVGFYVINDEKERQGDGREREKTQAFLLQRQLSLLKDIKSTGSSDLPSLLKTIDVDENLVFLQFHTPLAAELEASVNLFSLGSNQVRSIPFEIRFLGAEKLSVATYNNAPKMPAKLDELEDSGKSGSVNLKDVASGLQNLHEKLEKALYFLEGVKTGAIKVPDSGEEYEALQALSNLAGRITAIKRKLHESKSAGMIDSSSNADELNELVAVGSMLSSLFRKNMDYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLZ6</accession>
    <name>A0A1E3NLZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_15130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018250.1">
        <property type="nucleotide sequence ID" value="XM_019160680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLZ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NLZ6"/>
    <dbReference type="GeneID" id="30177367"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="5">
        <location>
            <begin position="477"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="599"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="606"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="653" mass="73881" checksum="D3C0A05E46496C12" modified="2017-01-18" version="1">MASNSLITSEFSGVPVTGIAFTNISLDKPVLKYQPLSNTSSETVTPLPLDATSRPSTINWIDETYFVVLYDNKPYFELMNSNDISRIDQHVDLVISSQPASCSCSTYDAKRKVLYLLDTSNRLYTYSFDSTSDSVLEETSNFVVTELTESISKIALHPSDDDKLLLLSNTLYEFNLESKQLERSINLFVEKVNCYDVIDNTIIVSSNNDRFINLIDLIQFKVQKIFVMNAPIVKFVINKYKNKSILAAIDQDGFVEVFKEPLAAAQNTESSMTPNSKRRRRNANKMVASVQPTSTIKLVNDQKSFSKIDNIIFDHDQLTIAYLQNEIYFITDKFNWFINKFDEAEITVLRKKGSLDSLKLRTEDKASLKTYNENNQVTIRTGDNFIDLDPVVDQEETGKGDDDEEDEEDNGDDFSTLVSRLDKSTSDLGTKLPSLLRNGTKHNESNKFRFKVGTLTTNLSQALRNNDNAMFDVIINNTTEENVVRSTVSQLEQHFVLKMLDKLAELVYKNRFKNTTEGAEYGMGNSSIGLTTWIRYVLIYHGTYLIGAAGGNGDLRRRLGLLGMSLERRASNMNRLLELKGRLSMVTVKVAVTRELENLGTNGQPEYDEDDVEYIEDEEDALTENDSSEDEHVEDEGIEDDDDDGDDDDDVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM37</accession>
    <name>A0A1E3NM37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15158</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018284.1">
        <property type="nucleotide sequence ID" value="XM_019160690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM37"/>
    <dbReference type="STRING" id="763406.A0A1E3NM37"/>
    <dbReference type="GeneID" id="30177377"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF66">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="673"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="704" mass="81112" checksum="FD9F54FBB7156DBB" modified="2017-01-18" version="1">MLLRPFFPIEEKLSAKVSCLDLVLLLIEIPSSKVRKEILGNLNICELLVVLNGLTKDEKNDDFLEFDELNADVKPVWTDVVEVLNNFPCVKLVDSLTKAVGDAADGLGWFDLPIDSNEYDFLLEYLLDSKVNRIVFPLVEIMTKKFNKIYEVLENHCVALKIRNDNLSVIKDSENLKSKLRILTLVNYEHFETVEKLNYETLNIVGNEVDLFSVSTMIKKNENLKIIYFKGRLKDYIPLHIDDKMLVYYDLIVFDTIDNSQLSDLGSLTRIESLYLNFTLESNVSLDWLLKNCTQMKRLYYSLNFNTNLTQTITFTENFTKLEELTLKRCEFTIQNDGYKRSKLSFSDAMNNAVSSLHTLTLIECKIDSEFLDAFCGPSLMSIFLINCDIRYNSFVRLPVGLRNLTIINQDVDKCKNLVFMTFKKRSSFILSSSKKCMININEKIMTMKIPESHDTFMARLNEDYCITDGENKNNTISPYEKQSFSIQNLKFPFSSTIHFAIYPNFDAVDFKFMKVLAAKFDIFQTIIELYSFTNTFHDVRNVQEIQNVKVYRYDILRLQNHLKKILSKKVLTEQDNKFFSPYPCSCESPLRLSPASVSSAARKLRSPKSPKKPHSLALKALNTVNNRVSSTNTKSQNGKIKSHYACDVCGKYFSRSTALNRHTLVHKNHRPYKCEGCGYCFKRSDHLKVHKRVCTSESGGSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM99</accession>
    <name>A0A1E3NM99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_109405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018377.1">
        <property type="nucleotide sequence ID" value="XM_019159732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM99"/>
    <dbReference type="GeneID" id="30176419"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="86" mass="9647" checksum="F1B2E2DA338427AC" modified="2017-01-18" version="1">MITMLYFNCLDKCPLPTCCCFPLSLPQSAHLIVSLGTMSLVPYPMRVHGSSSAMKFFILTNSCLLMSSGVVIFPFLKLNNPEYQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMB3</accession>
    <name>A0A1E3NMB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15188</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS33 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018319.1">
        <property type="nucleotide sequence ID" value="XM_019160706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMB3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMB3"/>
    <dbReference type="GeneID" id="30177393"/>
    <dbReference type="OrthoDB" id="2257454at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="118" mass="13244" checksum="C87265A0036DBE1B" modified="2017-01-18" version="1">MSTVIKGAAKQLVTPSKQKLFQLEQLQCQVFRTTFNPTKMKTGAKILTAPLKGQTLSKYYGPSDFPSIAKVARSLSTPGFRVINEPEAYRMERVEDLKRRGKGSPKKIREAASKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMR3</accession>
    <name>A0A1E3NMR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Meiotically up-regulated protein Msb1/Mug8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15328</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018479.1">
        <property type="nucleotide sequence ID" value="XM_019160771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMR3"/>
    <dbReference type="GeneID" id="30177458"/>
    <dbReference type="OrthoDB" id="3362494at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04401">
        <property type="entry name" value="RhoGAP_fMSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037508">
        <property type="entry name" value="Msb1/Mug8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012965">
        <property type="entry name" value="Msb1/Mug8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093:SF1">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08101">
        <property type="entry name" value="Msb1-Mug8_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meiotically up-regulated protein Msb1/Mug8" evidence="3">
        <location>
            <begin position="47"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="508"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="711"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="750"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="868"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="948"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1219"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="456"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="750"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="779"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="813"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="900"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="948"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="993"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1069"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1116"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1126"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1143"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1157"/>
            <end position="1167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1268" mass="142180" checksum="4ADA1CBA7FD25509" modified="2017-01-18" version="1">MNGEDVAKESFIYSPDVAPEALREFKSTYKRKTDEQYLLEKKYDITFERKHIKDLVHLITSEIKKKGTKTPMLMLPFRPAHHDVDLKTFLNSVFYRGMPVEEQVAKKIIRKTDECILMSALKFLWCRLPGKAIIGWKAYTKFVKLEDSAGFPDKAFLEFMPNCLSSGAHASIVYDFFDLIVALVLNSKENLMSARKLSKMCGLWAFNPVRNQASGAPSFERGLYEWIPAGDAMYHLLLSFVKAMPPNGDVTRLPKLFQVLLKSSDYPPLPTSATLETSRPLQEIPMVTIRANTPSANPAELLTRVSKTLKFDDATLFYTREDFLLLKRLFKEKDQVVEKLSSEGTRLLENLCLYDSDLMCDGDNSSSLKFKLVPGWSTDMTSRKLAPSKKGVHEIFTAVIGRASIDDYFIWTWLASLGVEETDLKKKTFGKTYIMEVELAEGFKKWVIVEEQDLERDGYDIELEIKQEKLKQLEQKIQLAEIEAKKVKQDAQHEKKKLVLEKVDVKELKTQQPTSLPPPPVPRKDYDADEIPPRSKSRKPPPQNGTTSSNVSGSPAPAPTMIPPPRSDKRAESPHYMEIQEPEPQSMPAKRYATKVVNNEEIRISLPILDAEDTFLRFDALGISEADLQSPSKTQAHLYVPAINSNEYAYGNGFGNNRFTNGENNENVSSNGMMNGNHNGFTNVDENGNLNYANGVHTNVKHANGAQAQRQMYTPSNGTPSPTNYYSPLQSPENSAAINYEMYMQSPTKGQFSDQIYPDQQHNNKQSPPPSRSPRQKYSTSPVSKSPQMSSSPPRSASPALNMKYQGSPRGYQNSPQQIQPQRSPIQQQNIQPFVSPNVQPALQNLQSPTHQSADQTAQQLPLSPIQQQIPHPVPNTAPSSRSFGNECEKIPNLPPPPEIGDRTPGDMASGERSESELTALSRSPVINEIDELEVELKDCMDDLENSSDTLTVQSRNVSNSSRLRKAPPLADPLNFSVSSESSSAYPATTIGGRDHHTKFSEDLRNAPLPAKPNATIDGPQQGILPPKQAVRNSSNPNRALSPAQKPNHQLPARHFSPQPHHSQQYPPQQYPPQQYPPQQYPPSGYPPQYPPHSPQGYPPQLFPPQGYSSQGFPPQGFPPPPQQQYPPRQYSPQGYFPPPTQFSPRQYPPQQFSPRQYPPPQFPPNPAGKRVSKSPRNQMPPHYASPTPNMGKPFPPYPQGVYYPPMPPNQFGVQIAHPSHEEQKTSSHSKNIIQHLPPSNRVDKLHGPQNLNKRNARDAFMTGNFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMS0</accession>
    <name>A0A1E3NMS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphotransferase</fullName>
            <ecNumber evidence="13">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_15353</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="13"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018514.1">
        <property type="nucleotide sequence ID" value="XM_019160786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMS0"/>
    <dbReference type="STRING" id="763406.A0A1E3NMS0"/>
    <dbReference type="GeneID" id="30177473"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000805">
        <property type="entry name" value="Hexokinase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000004">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="14">
        <location>
            <begin position="8"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="15">
        <location>
            <begin position="209"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="466" mass="51920" checksum="C7C7E3E2AF8D195D" modified="2017-01-18" version="1">MSLLDAYEKTKQELTVTPEKLTEITNHFVSELNKGLSKTGGNIPMIPGWVMDYPTGQETGDYLAIDLGGTNLRVVKVSLLGDSKFTYEAEKYPIPVPMRTGKSEDLWNFIADSLDTFVKKHYGSRGSSMAKLPLGFTFSYPASQDAINEGVLQRWTKGFDIDGVEGENVVPLLQDAINVRNTPVEVVSLINDTTGTLVASKYCDPETIMGLIFGTGCNGAYYDVCNDIPKLEGRLPDDLLKENSPMAINCEYGAFDNEWAVLPRTKYDIQIDQESPRPGQQYYEKMIAGYYLGEVLRLILLDYYSQDLIFKDQNIKPLQVPFAMDTSFPSRIEQHSATFVEGLFLDSFNIKTTEEERNIIHDLCLLIGTRSARLSVCGIAAICKKRNYKAGHCASDGSVFNMYPHFKERAHEALNDIFQWNCDPKDYPITLIHAEDGSGVGAAVIACLTEKRLLANKSVGTIESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN19</accession>
    <name>A0A1E3NN19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_24281</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Lipid-anchor</topology>
            <topology evidence="10">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018613.1">
        <property type="nucleotide sequence ID" value="XM_019162345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN19"/>
    <dbReference type="STRING" id="763406.A0A1E3NN19"/>
    <dbReference type="GeneID" id="30179032"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000032">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF5">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="361"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="402" mass="44205" checksum="D070B9918284B19A" modified="2017-01-18" version="1" fragment="single">ALAASCSALDPIVIKNNTFWVGETDERFYIRGADYQPGGSSNLTDALADSDICSRDIPYLKELGLNTIRVYSLDNTLDHDECMQKLEDAGIYVILDVNTPKASISRWKIECSYNTKYLTEVFATIDSFAKFNNTLGFFSANELVNDEDSFQYSPYIKAVTRDMKQYIKAQKYRTIPVGYSAADVSSVRYELAEYLNCGDDQDARIDMLGINDYSWCGRSSFSVSGYKEKVTDYTGYSLPIFLSEFGCNKVSGTRPFTEIGTIYSTQMSSVFSGGLVYQYFEDVNKYGLVDVDGSSVSILQDYSNLQKMYNTTSNPEGDGGAAYYEHSECPTNLNFSTAIPPQPKGLSKLIKLGPQGKNTGFKADTQQICSDDDDDTSSAGSSAVSSVISSAAKSSTSSHKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN97</accession>
    <name>A0A1E3NN97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_15515</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018692.1">
        <property type="nucleotide sequence ID" value="XM_019160858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN97"/>
    <dbReference type="STRING" id="763406.A0A1E3NN97"/>
    <dbReference type="GeneID" id="30177545"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000001">
        <property type="entry name" value="ADP,ATP carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="113"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="213"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ47579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="305" mass="33519" checksum="570DE4EE65F2A13E" modified="2017-01-18" version="1">MSGEKKLQSNFWTDFLMGGISAAVSKTAAAPIERVKLLIQNQDEMLKQGRLAKRYEGIGECFSRTAKEEGIVSFWRGNTANVIRYFPTQALNFAFKDKFKKMFGFKKDEGYWKWFAGNLASGGLAGGTSLFFVYSLDYARTRLANDAKASKGNGERQFNGLFDVYKKTLASDGIAGLYRGFLPSVVGIVVYRGLYFGLFDSLKPVILVGSLETNFLAAFLLGWAVTTGASTASYPLDTVRRRMMMTSGQAVKYKGAFDAFSQIVAKEGIKSLFKGCGANILRGVAGAGVISMYDQLQIILFGKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNM3</accession>
    <name>A0A1E3NNM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_21051</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Lipid-anchor</topology>
            <topology evidence="6">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018236.1">
        <property type="nucleotide sequence ID" value="XM_019162182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNM3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNM3"/>
    <dbReference type="GeneID" id="30178869"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF4">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012272278" description="1,3-beta-glucanosyltransferase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="412" mass="45637" checksum="EA74C5075CA1CA0D" modified="2017-01-18" version="1" fragment="single">TSLLCLAAFLDPAAALLPITLKGNRFIRPSTNSTDGSVFFVNGVDYQPGGSSAYTYNMTSDVLTDPEVCARDATVLQNLGVNTIRIYTIDPDLNHDECMSIFNSAGIYVILDVNSPLGGESLNRDDPESSYNAYYMSRVFRVIESFRSYSNVIGFFVGNEVINDEPSAGKDPKFLRAVTRDTKQYIQNRLADDDDAREIYVGYSAADVVSLRMPTFEYLTCAINGNDSDVSSIDFFGLNSYEWCSGSSDWQSSGYSKLVSGFENSSVPVFFSEYGCNKNSPRTFDEVSGGIYNDKLIDILDGGLVYEYSQETANYGLVDISDDDDSVTLLQDFFNFQKQLAKAQIPTINETEVVDVPALECNASLIQSYDKSFSANFTLPEANKDIKWMIDNGVGVSYKGKFVDVDQYLNPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNN5</accession>
    <name>A0A1E3NNN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_71743</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018817.1">
        <property type="nucleotide sequence ID" value="XM_019163994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNN5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNN5"/>
    <dbReference type="GeneID" id="30180681"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="68"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="112" mass="12843" checksum="24BE9216130BD76D" modified="2017-01-18" version="1">MLTFCPYCSNMLIIARSEESGSNAFTCPTCPYEFPIMGLQMFERKVLPQKEIDDVLGGAGSWDNVDQTMAQCPREACGNDRAYFFQLQIRSADEPMTTFYKCTKCSHQWREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNQ3</accession>
    <name>A0A1E3NNQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15637</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018842.1">
        <property type="nucleotide sequence ID" value="XM_019160900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNQ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNQ3"/>
    <dbReference type="GeneID" id="30177587"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05652">
        <property type="entry name" value="M20_ArgE_DapE-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF8">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133441" description="Peptidase M20 dimerisation domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="7">
        <location>
            <begin position="236"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="423" mass="46013" checksum="E789FB2B53AB7F7C" modified="2017-01-18" version="1">MVWSSALQVLALTFVASAAPAPVAAPHALIPAFLSTVMESSEQLGLLLGEELEDKFEKDELDAQFALFQKNPELFKLHKKLVNIESISGNEVSVSNYLQNYLSQLGLGLELAIDETGKNIYAYGSENGSTKVLLTSHIDTVPPYIDYFVQETEDGDVEIHGRGSCDAKGSVAAQIIAAKELLEDGIVTTDDLSLLFVFAEEVGGAGMRSANSYLVEEGINWDAVIFGEPTENKLATGHKGIYLAKLEVFGVASHSGYPDMGIDADKIMIEIMHELETYNWPTSELLNSTTLNIGMFGGGTAGNVISPYAECTILMRTAVDADEIDTIVQSIVSKHESKAKDLKLDVRVTDDPVSLDYEVDGFETIIASYSTDIPNLKQRGFKRYLYGPGSILVAHGDHEYVTASSMLQAVEDYKKLVLYSLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNQ8</accession>
    <name>A0A1E3NNQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_21273</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018839.1">
        <property type="nucleotide sequence ID" value="XM_019162191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNQ8"/>
    <dbReference type="GeneID" id="30178878"/>
    <dbReference type="OrthoDB" id="1930760at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="344" mass="38136" checksum="86B06985CF00A32C" modified="2017-01-18" version="1" fragment="single">REALFKTNSPPCCLRINPLDPSIVYFGTYTLIEGNNRKGSIEIWRIGKKKDVDSSFSTDFPDDDSSLLLATASSTGNITFWKIEKDDGTAFIEVADVQLFPEATLRSDETLITSINFHSNKNYLVFTATSGVVGYYDYESPTKFPEESSVRFFGSEHSLEAWFADWGHHGLLDNVVFSGGDDAKLIAHDIREPMAIMETSRIHDAGIVSILTGRQSWGEKVTDQFVIWTGGYDDNLCVLDLRAGLGSTGGSLIPGLPPIVREKHNLGGGVWRMIPSPHTNDSRVMTCNMYDGGRVLSYNEQTAQQVEVVGNYKGEHSSITYGGDWVGDKIISCSFYDNIVQVWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNS2</accession>
    <name>A0A1E3NNS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_71777</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018858.1">
        <property type="nucleotide sequence ID" value="XM_019164002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNS2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNS2"/>
    <dbReference type="GeneID" id="30180689"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000199">
        <property type="entry name" value="tRNA-aminoacylation cofactor ARC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 12" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="14">
        <location>
            <begin position="186"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="748" mass="81194" checksum="E7D1EFA42DA4DC68" modified="2017-01-18" version="1">MAFSKEQQQAAVSQWESLAPHIEDHVKELNETLKANTYVAGTGVHTDADVKVFKAVLPLATATLVKDVAANRHILRWIDLLQNTVPGLSESKLALDLDAALPREIKEKPKKKEAEGAAVAAQGKKKDAGGAAEAAAAADAAVPKGKPVDAEAMAKAKAAKEAKKAAKAKLRAQQEAEAKASQAGPSPSMIDLRVGYIEKAVKHPDADALYVSTIQMGDEDGPRTVCSGLVKHFPLEAMQQRYVIVVANLKPVTMRGIKSSAMVLCASSPEGKVEFVNPPAGSQPGDKIFFEGYDGVPEKVLAPKKKIWETVQPHFTTIDDFTAVYREEGKPDAKLVNKNGEPCKCSTIVGAEITAGMKLVSKMTNKQGGSVVLVPEDADDLWTLYNLISKHDEVSLKTQRNVRPIGSSGVPIPGAPAQKKIVKLTLDVLDIDFVASERELRIKGRTLNALADVPLGSHHTATVMLKESVKVHKDHWDQHYDDLVEAACSLGSKAEVGAIVLEEGVSHVCLITDSMTILRNKIEKSIPKKRRGDSSAHDKALDKFLTNTALSAIRNLDLLNLKAIIIASPGTLAAQLMKIIYQEIDKTGDKELIRCKSKFLTASSSTGFLQGLEEVMKDPTIKKQLADTKVQRNVALFDEFTRHLNNDDSKAFYGEREVEKAVEIGGAVKVLLITDSLFKNDDVQKRKHYIDLVEKVKHDAGEVSVFSSLHDSGKQLDQLTGIAVILNYPVPDIEDDDDEDDDGVYVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNW6</accession>
    <name>A0A1E3NNW6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018880.1">
        <property type="nucleotide sequence ID" value="XM_019160913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNW6"/>
    <dbReference type="GeneID" id="30177600"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="134" mass="15311" checksum="3B23DB27AD5761E2" modified="2017-01-18" version="1" fragment="single">MERFVRRVQDKIVETLTSSVFGETRASKEPDRESEKAEEVSPEQQEERSTGGNVKENNEVCDTENCVVRRNDNDREKLFVDSAEDEVPDANNDSIEDGKCHVKSRSIHGGSSKQKHIFLEADKKLIKMHKVIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP22</accession>
    <name>A0A1E3NP22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_31285</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018541.1">
        <property type="nucleotide sequence ID" value="XM_019162688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP22"/>
    <dbReference type="STRING" id="763406.A0A1E3NP22"/>
    <dbReference type="GeneID" id="30179375"/>
    <dbReference type="OrthoDB" id="65716at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071466">
        <property type="term" value="P:cellular response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000002">
        <property type="entry name" value="Zn cluster transcription factor Rds2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053045">
        <property type="entry name" value="Zinc_cluster_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986:SF7">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="67"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="190"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="483" mass="54587" checksum="46E3E16068F26DCA" modified="2017-01-18" version="1">MKPTSVKNDTNSPQHSGPPSPADAGPAKQNKKRKKVGSACVYCRRSHMNCDESRPCKRCIKRDIGHLCHDDGPKPKPKDGSFLTTNDPHSPDGSKNDTKTASSGALDNQLASSLQANFLPSSTALNQQPFFYSEHAGSEFNSLTEFLSMIDDNDMMDTISLTNDPMLQKLEATNYLSGFTNSTANLQNMMNHQLNGPSQSHQFPMEDGKLQQQQKQQQQQQQRNLPYEATNYQDAPFKTPSQQQAQLFSQKSPTLQKAPQNTPHISDAARDKFFLTAADPTTEISPEERLKQVINAKLEAGLLKPYNYAKGYQRLQTYMDKYMATSSRARILKPLSTFRPGFRAIAKTLKDIDLILVEESFERMLLDYDRVFTSMAIPACLWRRTGEIYRGNKEFASLVDVSIEDLKNGKLTIYELMTEESSVNFWEKYGSIAFDKTQKAVLTSCNLRTKDGRKKKNCCFSFTIRRDRYNIPSCIVGNFIPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP83</accession>
    <name>A0A1E3NP83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SIT4 phosphatase-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_23847</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018621.1">
        <property type="nucleotide sequence ID" value="XM_019162326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP83"/>
    <dbReference type="STRING" id="763406.A0A1E3NP83"/>
    <dbReference type="GeneID" id="30179013"/>
    <dbReference type="OrthoDB" id="295029at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="84"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="755"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="795"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="110"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="820"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="852" mass="97423" checksum="1F1F250B1883C1E1" modified="2017-01-18" version="1" fragment="single">MSGFWKFTSNYASRVTKILEAEDISLEDLLDEKETVSELLASNARLIEYLRRPEILEKLVDYVVEDDEYQRKEIELDLEAQNVDLTSSKETPEEKEKFVNKDQTQKSDAIEDDEDSDSSDDDDDDDEEDNDDDDDDDDDDSNDSDNDDDEEEEMINEEESEVEKHTRRAQVAAEILSIDVWSITDAFMESVHLLQQLWCILDKPAPLPIFSATYFMKINEHLLDMKTDEMIQFIHQEPNLVERFMRHIETPPLMDFLLKVISTDKPDDSTGIIDVLKDQGLIPSLLSFLSAEIPSSIQSAAGDFLKAFITISANNAENTTIGPNELTRELVSEPMIRLLVDMMLQGGTGLSNGVGIVIEIIRKNNSDYDPVPVVYVTVESHPPTPRDPIYLGTLVKIFAEKLPEFTKLLEKPISKTLDTPFGKIEPLGFERFKICELVAELIHCSNMALLNDQKGEELVKERDEIREFLIEKHKEMTKRVETDSSLSLEGTDMSKEIENLRLSRRTSEDEDVDKEKNDNSENEVSTGDNVDDEKLLRENPVVGDQLKIALYDNKIVVTILDMFFNFPWNNFLHNVVFDIVQQILNATMEIGYNKHLAVDLFDRCHITKLIINGHNRCIEYEEKTGLRLGYMGHLSLIAEEVVKFISLDIHHTTSNASVIDEAIDDIEWKQYVDETLADIRNKYNAVLGSDGKQMNEHDMNLFQRVYGSGEYDDEEEEDDDVVLEGNDNMVNGYMDSEGNEVGSDKFSRYIGEEITGDLPDRFGSSDEESEDDDIRQRRNQMWADDENDEIEGVVDSQMGGHPYHEMSADDSNSRYMVNDDYGDGYDDYEDPNDDGQSYKKMNHPLYQADGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPE4</accession>
    <name>A0A1E3NPE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018691.1">
        <property type="nucleotide sequence ID" value="XM_019160857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPE4"/>
    <dbReference type="GeneID" id="30177544"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="52" mass="6069" checksum="1F22ECA681426A10" modified="2017-01-18" version="1">MKFLILIQETPPGPQFRRVDARTPRATEKENFTSEPDQIRSDLAATQIELRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPF7</accession>
    <name>A0A1E3NPF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71999</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019114.1">
        <property type="nucleotide sequence ID" value="XM_019164069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NPF7"/>
    <dbReference type="GeneID" id="30180756"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF34">
        <property type="entry name" value="PROTEIN HOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="110"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="600" mass="67462" checksum="A392205278B24138" modified="2017-01-18" version="1">MSKRLSVETSSAAASSLFQQCTPFGMSIISAGFQRSAQLLEMSSLHPEKTREQILEQADQEWIPGTVFLVDVDRSLAVQHEGQSDIVLIPQPSKDPNDPLRWSQTKKNIQFAILFYWSFMTSVATNWNGPVWGIWVEIFETSYSVLNDTSAVCWCFLGLGCLFLQPTAMTLGRRCVYLFATLIQTLGNVMGGVAKNVGVLYGVNIMTGFAGAPCDSLVQISTTDIFFQHERATRISLLTFALYSGSYLGPIAAGYIVENNRFGWRWCYWFLVIFFGALLALQVVFMEDSTFVGQRENETEVEKEIIAQVISKEEGQVYVSASSEKDQNVTVFEEKDFAAPLDIPTQRTYMQRMGLIERKYNDKRPWWKIFIRPSLTLALPAIVWGGFVYGVQVMWLSLIATTQSEFFSVAPYNFSTAAVGDTNFSSLIGCFIGMFWGGPLSDKFVAWKARRNNGVMEPEFRLWFLILPAIINSGGLLMYGLGVNAGVHWILPAGFGMGCLGFGMGSGGAIVLTYALDCYPQLQSEGMVMILFLRNMIGMGFTFAIQPWLDTDGMVTTTWLMFMLSVVFNMSCLIFIMWGKKWRKMTKNLYLKFSDPHFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPK7</accession>
    <name>A0A1E3NPK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_31792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019173.1">
        <property type="nucleotide sequence ID" value="XM_019162722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPK7"/>
    <dbReference type="STRING" id="763406.A0A1E3NPK7"/>
    <dbReference type="GeneID" id="30179409"/>
    <dbReference type="OrthoDB" id="5562739at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR XBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="109" mass="12328" checksum="3F2F21B302EEB5EF" modified="2017-01-18" version="1" fragment="single">MGAVACEGVQSHPARDFVTVYEYKLGNHVILWDYVIGWVYITSIWKAVGNYKADVLKLVSTSPEIKNKVYKTKGGCLKVQGTWIQYDVAKQLAARFCWPIRFALVPLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ5</accession>
    <name>A0A1E3NPQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15858</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019119.1">
        <property type="nucleotide sequence ID" value="XM_019160997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPQ5"/>
    <dbReference type="GeneID" id="30177684"/>
    <dbReference type="OrthoDB" id="8963340at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140224">
        <property type="term" value="C:SLAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01205">
        <property type="entry name" value="EVH1_WASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21762">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000281">
        <property type="entry name" value="Actin associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033927">
        <property type="entry name" value="WASPfam_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="3">
        <location>
            <begin position="16"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="4">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="368"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="399"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="465"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="69087" checksum="AFD8A563955D9D8C" modified="2017-01-18" version="1">MTALTSADKDKIKRVVPKSSNKIIDAAIVRLYVNYPSLEEWTWTGLTGAVVLVDDLIGHTFFFKLVDVIGNQGVLWDQELWVDFQYSQDRLFFHSFEIEDFSVGFLFEDKSEAQHFFKRVQSRHKHASKLTVQNNNAVEARKRPEETSTRGYRGQESVADGVTNGGIVNEQRMRRSKGVLYYENEPPPEWRSFYKELENMGISEDMIAENREFIKDYIAKQGGPLVGLEPPIPRRYQNQIRDGGSGSGNGTLKASRSISQSLRSRSNTVNSGNSERKKKAPPPPPPPGSASSTGTSSATASTPPPPPYSNTNDVFLSPSRQDSYPDMEEIIEQETTTPASPPPAPAQSFSSASEPRRVHNVPPPMTFPSQSQSPMQASSAPSGPGLPPRLAPPTAARPIPSPPPRNGVPPAPPARGNRCCSTPPPARNPNAPAPPPSRRGPAPPPPPSRHTNANVAPPALPPGHPGYNPYGQQQQQQSPPQQYQQYHQQPPAQAQAPPQLPPQAQEGPPPPPPPPMSSFTNPPLPSSNSSAPPPPPPPPPGFPAMADTAPPRVETTGNGDRDALLASIRGAGIGKLKKVDKSQLEKPSVLLQEARGEPVSIPAVASAPGGAPGQPASLADALAAALSTRKSKVAASDDESDGDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX9</accession>
    <name>A0A1E3NPX9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_21317</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018716.1">
        <property type="nucleotide sequence ID" value="XM_019162194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPX9"/>
    <dbReference type="GeneID" id="30178881"/>
    <dbReference type="OrthoDB" id="1026733at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="4">
        <location>
            <begin position="298"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="397" mass="45964" checksum="6B342BAAA3BA1F0F" modified="2017-01-18" version="1" fragment="single">LSRKVNKALSVLFISLLLIIMIPLYILIRIFVHILFFVLSILVKIQSHGYKSIRNNDPVNISRRFIMRFDERIGNKSKNLLAADADASNVIDESHLVEIERPDFLECAYSHALYIVKKDIRWLLVYIESDQNREAVDFTHDVLINKKFLKFIKDRKILVWGGDISESEAFQTCNQFNITKIPFLGLFCLTVNQIPTSSGMQQSAPVLSLVAKIQGYKSLNATLNKLDRAYKKYNHTVNQLKLNSSSLHGAVRNLQGEAYANSLRRSQHQRQERQQNQEALRIQWLKWRKSTLVPECNERGEYSRIAIKLPDSSRVQIKVKKTCSLEEIYALVECTFLQQVEIDDSVVYEQPANYRHEYKFDIYSLVPRQMLPRDTTSVISENPLVYPNGNLIVEPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ16</accession>
    <name>A0A1E3NQ16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-binding protein RAP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_19581</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the regulation of telomere length, clustering and has a specific role in telomere position effect (TPE).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Chromosome</location>
            <location evidence="8">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the RAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019255.1">
        <property type="nucleotide sequence ID" value="XM_019162129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ16"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ16"/>
    <dbReference type="GeneID" id="30178816"/>
    <dbReference type="OrthoDB" id="435460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070187">
        <property type="term" value="C:shelterin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031848">
        <property type="term" value="P:protection from non-homologous end joining at telomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11655">
        <property type="entry name" value="rap1_myb-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021661">
        <property type="entry name" value="Rap1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038104">
        <property type="entry name" value="Rap1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015280">
        <property type="entry name" value="Rap1_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039595">
        <property type="entry name" value="TE2IP/Rap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466">
        <property type="entry name" value="TELOMERE REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466:SF6">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09197">
        <property type="entry name" value="Rap1-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11626">
        <property type="entry name" value="Rap1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0779">Telomere</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="103"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="507" mass="58313" checksum="12AE5247A2362DB2" modified="2017-01-18" version="1" fragment="single">NDHLKTMITSLGGEVVDDGNLDALALVPSKEKSRTDLAYQYLYQYIHDTFSKKTPLDINKYRVGKSSGQSLNINIGEFPIKPVQDEKLNENGDGIDANPFDFKSNKFTKEEDDFILDLVRRNPHLRSTHTFFARIAQLKPLSEHTGNSIRYRYRKVLAPNLAFVYKIDPKTGKPEIDAATNQPKKIEDIPSLIKSQYTSEEDYALCKHILLYKNGEMVLSGKKKHEVSQIPEAVFQELHKLNPRHSTMSWRDRYRKFAAKFGLRKYIAYYEECQQRDIEPEPMKNMSSRNDRKDYKVDVFGNESEERPSKRDEMKQYDIALADSQLEESNTNTDKKDAAEIMSEHVSPEISESTAAHLGEEGDNDLELVDVKADDGLMDFRQLIDIDPEPLKHRGEIDLSTMISNIRECFRNFGDGNTPYELFKDISDQTGISMLWLNYWFDCSCGMLGTFIQAIINYLKTGDLVMNDVSGFWTEKDDELLKVDPENKDLLHLHGKDSVTKRKAVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ77</accession>
    <name>A0A1E3NQ77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16071</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PAAF1/RPN14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019377.1">
        <property type="nucleotide sequence ID" value="XM_019161078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ77"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ77"/>
    <dbReference type="GeneID" id="30177765"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF19">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT RPN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="415" mass="46481" checksum="854C02AC8DD5D56F" modified="2017-01-18" version="1">MYNAPQVEIQSDALQICFELAKEISDRNPNTVWLRIGQNSFNLHVANGSMIDKEHDLKLVQEKNRPTCYKLFYGQNEPFNLVFPSAHFVLGVDKHLNLTRICGLPNMFVLGTSNGDLIKFGNPIPFNISKFVLKENAHYADVTHVLSFSSGSVLLTIGIDMQIKIWKNDSKDLDFGSPIRILKGIHQGRITDCVMIGRGRNIVSCGLDGQIVFWELGSGEAVWKGRRIRDLHDGCTSLCISKITSEETHVNSEMFFECDGKVLWSGHVSGTVSVWDCSTRLSLGEFPTNQDGYQVQQIEALETMNGVIIGLNNGNVILFTYDFNQRKAIKVWEVNVEKPENEDGTIDIKKIRAHKSLILVLTDNYLACLDLKTGHLISSFVGYDDSVNDFYVSDMGNTSKVVVAGKRGFLSMFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ80</accession>
    <name>A0A1E3NQ80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Triosephosphate isomerase</fullName>
            <ecNumber evidence="6 11">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_72184</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="6 11"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019325.1">
        <property type="nucleotide sequence ID" value="XM_019164118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ80"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ80"/>
    <dbReference type="GeneID" id="30180805"/>
    <dbReference type="OrthoDB" id="6715177at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF41">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="249" mass="26940" checksum="0232DB31693D539A" modified="2017-01-18" version="1">MARTFFVGGNFKMNGSKESIKTIVENLNSSKLPANVEVVLCPPAPYLAYTAELNKQKTVQVGAQNSYTKESGAYTGEISPVEVKDCGAEWVILGHSERRGIFHESDEFVADKVAFAIEKGLKVILCIGESIDEKKAGSTLDVCERELAAVVKAVKKDDWQHIVVAYEPIWAIGTGLAATAADAQDIHSKIRDYLKSELGELADSVRILYGGSANGKNAPDFRDKPDVDGFLVGGASLKPEFVSIITSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQC8</accession>
    <name>A0A1E3NQC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018856.1">
        <property type="nucleotide sequence ID" value="XM_019164001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQC8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQC8"/>
    <dbReference type="GeneID" id="30180688"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071341">
        <property type="term" value="C:medial cortical node"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="74"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="142" mass="15592" checksum="884A3EB17DEFAE05" modified="2017-01-18" version="1">MADERTLQDAFALFDKAATGKVSAGSLGDLLRAVGQNPSLKEISDLQDTLAGNEVDYDTFKKMVNREGGFKQLGKPEDYIKAFQIFDKNLTGFIGVGELKYILTTIGEKLSGDEVDELLKNVTITDENSVDYAEFVKSILSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQG4</accession>
    <name>A0A1E3NQG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_69986</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019453.1">
        <property type="nucleotide sequence ID" value="XM_019163523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQG4"/>
    <dbReference type="GeneID" id="30180210"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="3">
        <location>
            <begin position="11"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="496" mass="55258" checksum="2AF11D8C14E81825" modified="2017-01-18" version="1">MGRNGKSQQEQRLLELLNAPVNRNKCGECKSHDPTWASWNLGVFLCGRCASAHRSLGDHISVVKSLSMENWSSHELSVLGKIGNKRNNGFWNEKKIPFPFDPDDKDSLVMWLREKYTGKFRYGAVTDADYNLDDSWADRTDDYGFDNNRSRSSSSGGIMGRRISSALGLNYVGPTRRRQMSAASSSAGGLSNNDYGYDADDADSSTLTRSMRRGREKNGLRSGYDNDDDDYGSHGSRSRSTYDRDRPRGSRYTSPVSPSRPARLTFRRPTGTEFRKYGDQSRKMKFDMGFEDEDVNVEALALAHGNINRAIEIIKQSGVKPADHSTDAPSSASASARGTSTSTPSLPQRKETTGALFDSSKAGGFNWLDDSMPPAATGTLTGTAPASAAAENQIFQYVDPNTGAVYYIDGNGQQYVDPSLQQQQTQLLPQQQQQQTLQTNPYQMQQAQFQQQQALQQQQALQQQQALQQQQMLQQQQMLMQQQQQQTQQPSYFGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQH0</accession>
    <name>A0A1E3NQH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_13943</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019470.1">
        <property type="nucleotide sequence ID" value="XM_019160146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQH0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQH0"/>
    <dbReference type="GeneID" id="30176833"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="8">
        <location>
            <begin position="34"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="179"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="290"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="327"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="382"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="422"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="447" mass="50573" checksum="17343A37ECD21928" modified="2017-01-18" version="1">MSAVAVSTQMSPAPRAKINDVNESYQMNEMLINEEYKIWKKTSPLLYDLLCSYSCEVPALSVNWLQEFTIEKNDNAESFISAQFLMGTHSKDFQNHVKMVSVSVPPTLYNDYTGPSPSIPNNLLKSRQLNIVREWNHPGEVNKLRVNQFNKLFATQTNGGDILLFDLKDGDSKTHKSTLKFHSKEGFGLEWNPNVSKKNLLLSSSEDCKIALWDINEKLHPEMKPTRTISSHENIVNDISWSRSIDSVFASVSDDSSYQIHDLRLTTGPIVHITDAHHDPDSENNFNFPINTVEFSESVPTLFATGGSDNSIQIWDLRNPSIPLRRLTGHTAPVVGLKFHENYLLSSSVDNRVLIWDLNRLDEGEPDKRKADYVDPCLAFMHGGHTGKICEVDWHPVLENLIVSCAEDGLLEVWRPAHLGDEYIEEENEEEKEEGGTKSDDKKESSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQX6</accession>
    <name>A0A1E3NQX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019620.1">
        <property type="nucleotide sequence ID" value="XM_019163567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NQX6"/>
    <dbReference type="GeneID" id="30180254"/>
    <dbReference type="OrthoDB" id="4080456at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052772">
        <property type="entry name" value="Endo/PolyKinase_Domain-Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535:SF1">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="11"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="391"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="55612" checksum="53E6CA395C4B9B7A" modified="2017-01-18" version="1">MAEKRDKDEKELPEALFPLSELFPDVSLKHILHILRISNGNFDSAAEQLLSYDLIKDELDKLDQAEPTEPAEPEERLPPESFSDAVEALQVEQWAKRFELQDEIMELLEIDETERELVDWYLSQNSCRKLETVCDIMLNFDSRLPAAQQPRDKLDTASYNVLYLHGVDKSSPGVGTGGDTGSSNDHFLSMSDLLRKSSRGSSTLLENDRSWSELQKVIDDNPELNLPEKFYLVAMKWFHKDLDKILHTAIQLSEYFEKQPRNIMSADTSRIDFGVLAFGNNSLKDEITEYRQKQSSNGHGTISTKPMSIFENGSQPADLPKYSTYPPALEKLQSRAANLKQSRNTTGDKYLKSYYNHSIAETERKIRDLHGISQAQEVNQRVTQAKRTFKIDLHTLTVQNAMQALNSCLNYWWGQEMHLRNLENTKFEFTGLRHVDPFVIVTGRGLHSAGGIPKVKNATVGFLRQNGFKFEENTSIITVLGKRKNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR25</accession>
    <name>A0A1E3NR25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="5 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_70161</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="18">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="18">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5 18"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019649.1">
        <property type="nucleotide sequence ID" value="XM_019163575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR25"/>
    <dbReference type="STRING" id="763406.A0A1E3NR25"/>
    <dbReference type="GeneID" id="30180262"/>
    <dbReference type="OrthoDB" id="1696654at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="16 18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0408">Iron</keyword>
    <keyword evidence="15 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="146"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ48536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="243" mass="27693" checksum="00E7A7EE5E3934EB" modified="2017-01-18" version="1">MATAAAKTPNMKTFKIYRWNPDTPEVKPHMKAYDLDMNECGPMVLDALLKIKNEFDSSLTLRRSCREGICGSCAMNIGGRNTLACLCKLDKDSGKELKIYPLPHMYIVRDLVPDLTHFYKQYKSIQPYLQSDKPMPVRENLQSVEDRHKLDGLYECILCACCSTSCPSYWWNQQEYLGPAVLMQAYRWMVDTRDTATKARQAMLQNSMSLYRCHTIMNCSKTCPKGLNPGGAIAEIKKSMAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR93</accession>
    <name>A0A1E3NR93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptide hydrolase</fullName>
            <ecNumber evidence="15">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_14143</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in vacuolar sorting and osmoregulation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Vacuole membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019707.1">
        <property type="nucleotide sequence ID" value="XM_019160242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR93"/>
    <dbReference type="STRING" id="763406.A0A1E3NR93"/>
    <dbReference type="GeneID" id="30176929"/>
    <dbReference type="OrthoDB" id="76293at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053975">
        <property type="entry name" value="PFF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053976">
        <property type="entry name" value="PFF1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF58">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22250">
        <property type="entry name" value="PFF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22251">
        <property type="entry name" value="PFF1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="542"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="607"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="18">
        <location>
            <begin position="66"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease transmembrane" evidence="20">
        <location>
            <begin position="327"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease C-terminal" evidence="19">
        <location>
            <begin position="635"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="461"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="467"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ48594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="919" mass="104533" checksum="B4BA9995F1268A9F" modified="2017-01-18" version="1">MHPFTSAENDALHDYLGSVIDTVTEGVPYMKTSYDKNENHTILINQHDVFNKSNNDCRVIYYESSNILVKIEGSEPKLPGILVSAHYDSVPTSFGTTDDGMGIASMLGILKHFAESESQPLRTLILNFNNNEEFGLLGAEAFAQHKWFPDVSFFINLEGTGAGGQPILFRGTDKTVIDWYHSASTPYANSIFQEGFNSGFISSQTDYHVYERSGLRGIDIAFYHPRSFYHTFKDSVKYTSKGSLWMMMSNVLDILLDVTYSSESYDSDLEYSVYFDILNIWFFNFSLNLNFVVNVILLVLVPSINIILLLIVFKRNTWFIGLKGWSRFPITLIACYYSTLFVSKQLYNLNPLLISVNFYYPSMSLFSITILIGYLILKASAYFRPVHDQKLVVLLELNLLSWLSLVWITYKIKGYKNMGGYLLTLFYVLTSLASFWGLVGLSFRKSPCYQPNKTVIVYNSTNQSQDGNDRNSNDQPVHHDNGSEPAPHIHDINGEGSDTEQGDDNESTPLMNGTSVVINHETQDSFMHKVKHNAINSLQYDWLIQFITLIPLSIFFIYSDGRLVLEALHETVQENKLYDDAIWKLVALLSTSLAAILMPFIHKMNMITVQLILLLLAYGLFESYFSPSYNESTPLKLRFVEMFDVNSNSCLASVYGRQGFIPQILSEVPYLDPKNVSCSKNEWAGTEICSYEGNRPWLVSGKLEDNQYDQYLNVTVLSNTNTFRDVSDEAADKFTPVESVLEVEVRGTRQCYFTFNTTSTSFKAPVKMITVYQDGNSLTEEDIWSNASLDVDSEDKKEAAIPNGMSRDSKGNWIYKEMKGIDLLQLHKLQWANEGSNKNRFTVKFQWLPFHYDSDIELITNLGVNVQCHWSGFDDVVNVNGENHDKVQEYVDLMTYTDVGVAWTNFRPGVVQGNAYLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRC3</accession>
    <name>A0A1E3NRC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_70297</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019790.1">
        <property type="nucleotide sequence ID" value="XM_019163605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRC3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRC3"/>
    <dbReference type="GeneID" id="30180292"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000388">
        <property type="term" value="P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01683">
        <property type="entry name" value="EF2_IV_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000029">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000646">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031950">
        <property type="entry name" value="EFTUD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16004">
        <property type="entry name" value="EFTUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="117"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="11"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="980" mass="111101" checksum="1E081C8D37808558" modified="2017-01-18" version="1">MSDDVQFDEFGNLINGDLSDSSDEESQNLSNEINNDQLRENNDNDNAIVLAEDRTYYPTLSSTFDGVETIIATEDEKTLNDPIVEPYVEKKFMLEETDLPDTTYSKRYLWEMSKVPERIRNVAFCGNIHAGKTSLVDMLVEQTHMFSSKAQEAQGVKQTKLRYTDNHILEVKRGISIKSSVMSLLLPDQNETYILLNIVDSPGHSNFCDEMAISIRLSDVTVLCVDVVESVTKSLELVIEYALKTGTKMMLVVTKIDRLILELRLSPLDAYYKIRRTIEQVNETIEKYCDELEIDFAEAKLKLSPELGNVCFSSTTFNILFSLSSFAQKYIDSNNFNKNLGMSTELLARKLWGDIYYESKKFFVKPNNPIAAASSRTFIKFILAPIYKMATASLTLDPVQLKRFAELNLGLDLKKRVYKFDTRPFMKELFGSFFGPPSSALVSSLMKLPSPRANSNTKINHLYDGPPNSKLSEHANNCDREGPLIAFVSKLVDTTDSENFYALVRVLSGSIKENQLVSLLGESYSLSNQDDYKLQKISKCYLWSGRYKIEVPELNAGSIGLISGPGIDSFITKTATIYDQGIEDSWIVFRGVDQLLSPVFKVGVQAYNPKDLNKFLECLKKLHRSYVGCEIKVEDSGEHSIFGFGELYMDCLLHDLRILYGNLEIKVSDPMVKFNESTDSLSKVKLVTKSNNVKNQISIIAEPLDPKICKDIRNGTLSFKRDTPRNFAKKLRDKYNWDSLAARSVWSFGPTVTGTSILCDDTLPDEVDKKQLLELKEYILKGFQWAVREGPLCEEPVSDVKFRIINAQFAEDDADRNGAQIIQMVRKACHSALLISDVSLLEPIYHIETISHVDTLNMLEKFLDKRRGYIFDKERIDGTPLWKVTGFIPVIESVGVETELRLATQGKAYPQMVFNKWEQVPGNPLDDTAFIPLLKRVPLLSTSRDFMIKTRRRKGLSENVSLQKYVDEDTWKVLQGLGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRC4</accession>
    <name>A0A1E3NRC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_84574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019737.1">
        <property type="nucleotide sequence ID" value="XM_019165008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRC4"/>
    <dbReference type="GeneID" id="30181695"/>
    <dbReference type="OrthoDB" id="3998265at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="286" mass="30560" checksum="B76E306A1711AE8C" modified="2017-01-18" version="1">MHRSIQSQMMPITELADTLSFGLVGKIRRKTHVLEDDVQLVQEKWRSQDRATELHGCEARAAAAVEDSKDTITAAYSFNDSVIRQSMRRRSGSPAASSSSATLCDSGLKRSVSMQQPSVRDGRVEQRRESLPRCSIARSLDRDMHAFRRKAGDTISSLTNSGVSISDSATDLVSGTSFSVGTGSSGTLTPSKVPTPAAKSQYPVLGSGASSSGYNSHYYAGTQLASQDAEDTDTSSISSFSTSNEFRLVLDDQIIPGVSGTASAGNPQLQRVPGASRPQEMIFLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRI7</accession>
    <name>A0A1E3NRI7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019286.1">
        <property type="nucleotide sequence ID" value="XM_019159748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRI7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRI7"/>
    <dbReference type="GeneID" id="30176435"/>
    <dbReference type="OrthoDB" id="548474at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038966">
        <property type="entry name" value="TMA17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40422">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40422:SF1">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="146" mass="16886" checksum="2B1B3D8B38EF1D62" modified="2017-01-18" version="1">MSEYKRPISLMDFEIAVHDLSDQELASILEKLHVSISRLIQSNELMQSLMDKEKKKSEETHIADGEVADEDDEFDTPTKDDIKIYRDSISENEVVIENQRARCRIIENELELRNLPSSRPQDAQHYRSLQNSNDVPISTDNEQIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRN2</accession>
    <name>A0A1E3NRN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14263</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019847.1">
        <property type="nucleotide sequence ID" value="XM_019160300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRN2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRN2"/>
    <dbReference type="GeneID" id="30176987"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000131">
        <property type="entry name" value="GTP-binding nuclear protein Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="215" mass="24441" checksum="6F68EA27DD80D230" modified="2017-01-18" version="1">MAQQQVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYMATSGVEVHPMSFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINGQCGIIMFDVTSRITYKNVPNWYRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYFDISAKSNYNFEKPFLWLARKLVGNNQLEFVESPALAPPEVTVDSDLMAKYQAEMDQAAAMPLPDEDDADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS34</accession>
    <name>A0A1E3NS34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020060.1">
        <property type="nucleotide sequence ID" value="XM_019160385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS34"/>
    <dbReference type="STRING" id="763406.A0A1E3NS34"/>
    <dbReference type="GeneID" id="30177072"/>
    <dbReference type="OrthoDB" id="16824at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="55" mass="6344" checksum="9F58673BC5B286BC" modified="2017-01-18" version="1" fragment="single">MSGIKKNTGITPDVALIGEPNVEYYVTKYFDYGDFVYDNAVTFMKTSKYFVKNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS82</accession>
    <name>A0A1E3NS82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_9414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020017.1">
        <property type="nucleotide sequence ID" value="XM_019165091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS82"/>
    <dbReference type="GeneID" id="30181778"/>
    <dbReference type="OrthoDB" id="3995709at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="341" mass="38801" checksum="B27F78A7816251E9" modified="2017-01-18" version="1">MVLHNDKWKYKAKRNYERKHAIKSANKSFTKEKTANGSTDNDGESDSGSDNEGSGSDSSLESEEKDAGSDDEFKRRSKKNAKSSNSWRFEDPIVDETILKDPEYIARLEAIKVEEENRASYMRNLVNDKLKTAGEADEVDLDNKYSTPKEIKSMKKFKQTDLQNWKFDDNLSDSDEDGEAFEPEVRDFTPEEREKFLALQKKINHQKDVEKIRNRMDQLNNKSKPTKAKVLELHSKAGRDKYKAAVDKNLRNAHDKIDTDELDNLVQRMIGVDLKAHEQPELRKTDHGQFDLDTMLQSTSGTAKSESIGQRTVPSTGTTAAKKATVVLETTELDDFLDSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSB1</accession>
    <name>A0A1E3NSB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TIGR01456 family HAD hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_70194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019681.1">
        <property type="nucleotide sequence ID" value="XM_019163585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSB1"/>
    <dbReference type="GeneID" id="30180272"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF57">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43092" checksum="A826A8E681213917" modified="2017-01-18" version="1">MTASSSEIKPLSTPVEEVAQKLESPQDIGFVFDIDGVLLKGKKRIAEATEIITYLSKKKIPFILLTNGGGMTEKKRIDFINMTLQLTETPIHEDQLIQAHTPMKTLIPHHKRVLICGGPKDDVREVAEGYGFEDVIRPVDLIRANPTIWPYHMFTDEQIEEWGRDPASSKLDVDGTGCGENLPIDSVLCFNDSRAIGAELQVILDLLNSEDGVLGTRRTYKSGKPSIPIVFSNNDFLWSNEYKQPRFAMGALKIAVLTIYEKINGQKLEQLTLGKPEKVCYDYAHHILIDWRKVLLGEKQPGNLCLPQLNDPPINTPFKKVYMVGDNPESDITGGNNYNWGTILVRTGVYQEGDFERNPETSRPSLGIFPNVKEGVMAALKLNGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSG2</accession>
    <name>A0A1E3NSG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kynurenine 3-monooxygenase</fullName>
            <ecNumber evidence="11">1.14.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Biosynthesis of nicotinic acid protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Kynurenine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BNA4</name>
        <name evidence="13" type="ORF">PICMEDRAFT_14532</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-kynurenine + NADPH + O2 + H(+) = 3-hydroxy-L-kynurenine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:20545"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the aromatic-ring hydroxylase family. KMO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.9" evidence="11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020152.1">
        <property type="nucleotide sequence ID" value="XM_019160418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSG2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSG2"/>
    <dbReference type="GeneID" id="30177105"/>
    <dbReference type="OrthoDB" id="10053569at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004502">
        <property type="term" value="F:kynurenine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070189">
        <property type="term" value="P:kynurenine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000129">
        <property type="entry name" value="Kynurenine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01971">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027545">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028:SF2">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 11" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="4"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="450" mass="50893" checksum="050BDC756FA215A9" modified="2017-01-18" version="1">MSESVAVIGAGLVGALVALGLGHRGYNVTLFDLRDDPRTAGTAIKKNLRSINLAVSDRGIRGLEFVDSEMSARVLKEIIPMHGRMIHTLDGKQESQIYGLFGEHINSIDRAKLNESLLDEIDTFNKTAVNQIKLKFEHKLVNLNFNTRGASVECEVRYGVKRSFDFDFVIGADGSYSNVRYQMQKYIRMDYSQEYIDKCYLELYIPPGENGAFLLNPNHLHIWPRKEFMLIALANSDGSFTSTFFGPWSLTEALKKAEDIETFFLSNFPDAADLIGMENVVKAFTGNPKGALMQVTCSQYHFSDKCIILGDATHSMVPFYGQGMNCGFEDVRILLGLLDEKETREAAFDTYTANRHKDLKAILKLALDNYKEMSTKVISSAYLLRKTLDSFLGRLIPQYWIPLYTMVSFRGDIPYSQCVRREKTQQKILKCIELGSITVVAIAAYWKFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSL3</accession>
    <name>A0A1E3NSL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_29642</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020202.1">
        <property type="nucleotide sequence ID" value="XM_019162602.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NSL3"/>
    <dbReference type="GeneID" id="30179289"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="539"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="715"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1154"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1177"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1234"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1263"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1293"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1415"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="94"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="811"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1459" mass="166210" checksum="606E15A4D17E5C41" modified="2017-01-18" version="1">MTRLSTLSKTLSTKNANEMELFQMDADDFDLAKILKYLVNFNENNGYENNEVDVVFKDLTVIGKNNAASVLRDVSDVFFKPFVLLYNRLKRNKEQTFDFKSLPKTRKIIRGVTGFASPGTITLVLGRPGAGCSTFLKALTGEVQTYKGVEGTLTFNGIDCQKIFDNLSNTLIYNPELDVHFPFLTVEQTIRFAVACKTPTIRFDNKTREEYIENMKDLYEILFGLKFVEKTLVGSDFIRGVSGGQRKRVSIAEAMVTNGNVYCFDNATRGLDSSTALEFVQALRTSTNIYKTSSVVTIYQASENIYQLFDFVTVLYLGRQIYFGPIHDAVNYFENLGFAKHPRETSSEFLTSITDPLARKIDPSFKDKSLIPQTADQFEAVWRNSEEFKSLQNQIDERLSEKNDGDTEFHNLKQNHELIKQKYTSKKSQYTINYLSQLKECCNRRFHIAINNRAYPVTLIFAAIMQSLVTGSLMYNITSETVGAFSKGGVLFFSCLYFALMTLAETPALFLDKPILNKQYGYKLYHPSAELLAKHLVQFPVRLIAITCFAIIIYFLTHLKTEAGAFFHYYLFINLVVQAVSGLFVMLASLMPNASAAMGICGISMISMVIYSSYMIQRPSMYWWFKWFSYCNPVLYAFESMILMQFHNLRMACSPFELIPFGPSYANINSRLNQVCGFVGAAKSKPLYDGANDVKGDIYLELAFTYTFNHVWRNFGIMFAFIIGYIAISCLIVENFNPIVASSDQLLFIKNAKISKRMFEHLRQLAAEDDNEVDLEKHEDEDFLVTVDSLTNSSHEGNSVNSHKLGSNNIFTWKNIDYVVPYEGEERKLLEDVQGYVRPGSLTALMGESGAGKTTLLNVLSRRTDVGIVTGDMLINGKPMGQSFERRTGYVQQQDLHIAELTVKESLLFSARLRRPMSVPDEEKIDYVNMVMKFLHMEDYADSIAGISGYGLNVEQRKKLSIATELVAKPSLLLFLDEPTSGLDSQSSWAIVQVLKDLAKAGQAILCTIHQPSATLFEQFDRLLLLKKGGQTVYFGNIGPDSRTVIDYFEKNGGRVCSKEENPAEYILDVIGAGATASTTNDWFDVWQNSQEYQSVSAEVDHLVQQGLTIQDEESDNADLDSTYATSYQYQLYQVLNRTFIQLYRSLPYIMPKFMLTLLGGLIIGFSFWNVKHTVIGMQNVMFANFLTLIISAPIMNQMQTKAISSRELYEVRESKSKTFHWSCLLISQYVNEIPYCIVFSTIFFVCWYFPIQLDNEPSRAGFWWFTYCFFYQLYFPAFALMILYAAPDLPSANILLGLFFSFTISFCGVVQRPQLMPGFWKFMWRLSPLTYFIGNQVGTLLHDRPVHCSEAEFNYLDPPDGLTCGSYLNEYFSTNAGYVENPNDTSNCAVCQYSVGDDFLHSIGVSYNDRWRNIGLFCAYICFNLFLMNFLYFVFRVKRFSPFNLVRFVKSFKNKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSY9</accession>
    <name>A0A1E3NSY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-phosphotransferase</fullName>
            <ecNumber evidence="3">2.7.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70821</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-phospho-[ligated tRNA] + NAD(+) = mature tRNA + ADP-alpha-D-ribose 1'',2''-cyclic phosphate + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:23324"/>
            <dbReference type="Rhea" id="RHEA-COMP:11106"/>
            <dbReference type="Rhea" id="RHEA-COMP:11107"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:76596"/>
            <dbReference type="ChEBI" id="CHEBI:82883"/>
            <dbReference type="ChEBI" id="CHEBI:85027"/>
            <dbReference type="EC" id="2.7.1.160"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.160" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020380.1">
        <property type="nucleotide sequence ID" value="XM_019163750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSY9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSY9"/>
    <dbReference type="GeneID" id="30180437"/>
    <dbReference type="OrthoDB" id="419694at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="245" mass="27344" checksum="9617555D85642DB9" modified="2017-01-18" version="1">MSEIRSTKRDIQISRALSKLLRHRAAAQGLAIDSSGYVSVSDVLQHNDLKCKHATVEDLRRVVETNDKQRFRLAEKGADGALYICALQGHSMAAVNAGADLKRMDQRHDEDWPQYIVHGTYRDKLPLIKRAQGLSRMGRNHVHFSYTLPEKFQRHLPPASEAASPKAAESHRAISGMRSSCQVALLLDVDRIRNSELEFYRSANGVILSAGDSRGIVDARYIAQIVDSEHGVLSWTDVPDCRESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT50</accession>
    <name>A0A1E3NT50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_14659</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020299.1">
        <property type="nucleotide sequence ID" value="XM_019160475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT50"/>
    <dbReference type="STRING" id="763406.A0A1E3NT50"/>
    <dbReference type="GeneID" id="30177162"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF162">
        <property type="entry name" value="TRANSMEMBRANE E3 UBIQUITIN-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133488" description="RING-type E3 ubiquitin transferase" evidence="17">
        <location>
            <begin position="21"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="588"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="611"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="753"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="528"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="540"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ49186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="847" mass="97099" checksum="815705DBB4BE7E94" modified="2017-01-18" version="1">MNSQTRFFFFMLIFLWVMSSSPNIEERYPSYIDRPHILQQFKSEVNISRNALLSNYNAGYGNLTGFHLSYKDALDGRNASDWPFPGKSGLFVEEEKYSILPNSVSRRAANVWNQEGKCVDLPSSRDDDNKERVGTKLNTGDFPLNISGLVRGRFNRIDNTNELLTPIHMPLPNYLSKLYEYRTDERRSKEEEKRNLNNDGDDDNYSTPIDVDLQNNEYMKKIGNFTEPEGLIKLSFYNSYPESSTDDLDFEDTTTVRVSVRLNDLTEKDEHILALSGVYHQDTGNMVVTTRSAKFDGIHALPELNLAPGSHFNKSRWALYEEFNRTKIDDLKFQQVERLVDASDECEYIGYFHIESTNLTKEELKEVDYELLNPIGRPHRPVPDLKLSSGILYSPNCAILLNLETARGLRDEISDNSLRNTILVAAVVVLFQIWILIRQMAQTNTPSTLSKLSFWTISIINMADGSISVISLLCSMIYTELYIQFAVCSFLAFTCSAIYEMKYGIQIYCTQLNERPLDWRTMLQGTPVDERSERRDRDINNTGDNTNNPNAAANDDNGANTTNFVTPTNPAGTTDEQAVGAELYTRHFFTMLVFLFVLMNVVTWPKTPRRVFEYIFITLFNSFWFPQIYRNVLRGSRTSFSWEFILSTSVLRLIPVIYIDLFPNSFHHHRDIGYVIFLFFWMSFQLTILLLQELLGPRFFLSDKYLPATYDYHPIITKGDIESGFNLDAEELVSDGSSSTDEDSRKLKYVTDCAICMQKVEIPVLDNGTAESTHSHNESQGEHSSLLNTSLIPGSSGIGFGKGTVNLIARRRYMVTPCKHVFHTECLENWMMYKLQCPVCRNSLPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT51</accession>
    <name>A0A1E3NT51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein STB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_98247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020365.1">
        <property type="nucleotide sequence ID" value="XM_019165134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT51"/>
    <dbReference type="STRING" id="763406.A0A1E3NT51"/>
    <dbReference type="GeneID" id="30181821"/>
    <dbReference type="OrthoDB" id="5391991at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000432">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018818">
        <property type="entry name" value="Stb3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164:SF1">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10330">
        <property type="entry name" value="Stb3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="629" mass="67020" checksum="68DBD02CA95A6777" modified="2017-01-18" version="1">MYASSVDTPGSNGSGVAEGTPSGIAHHGVFAGSHVGSNSNNNSTATSRGTTPQIHNLLNLTSSDSTTERNNLSSMSLPLSSNSYSAASPSPPHNDTNTVNSLSPPPQTESNDAFAAAAAAKAKQKRKPTRKLSSNEEKVLLSTSSPEGIAAASQITPNRIANILIKEGPLPIRHLTGYLIQQVPAFGNLSLSKQRRLIMAALESGDILTGCVFEKIGWGQWEAKIVGKELVKIKIENSLNNNNNSSGTNTNGNSNISTPSFANGKDSGHNGVNNVSSGNATSGSTAIKNLDINNNAPPSTQNMDIDRQSLYSSSEHAHISQQQPGFYHTSIKSESAIADPKLKPSKLLSDSIRRESITSQSNDGTFKVPTSPTLGPIQNLRNSFKNYADIDEAIESSSDDEFENDYDDEDFNLNNDSGGSPPAIMESLHNGTSTGTSNIAIPTTATTNNSANNNKGLPNTLPKNKVQSIRSPSVSSSRRPSFAGILKPRKPRSSFNQHTLEVALDEGPLERRESRVSFSNSASLSRQSFLRTNISPRLSNNNSSASIHDKDNENAIADDDEEEDGNFTDEEDWQAIGPSSLRKNRHLSIVTPPTPTLLDSAAGISDNKPDSSKTDEEMAAIALMDLKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTC2</accession>
    <name>A0A1E3NTC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70860</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020423.1">
        <property type="nucleotide sequence ID" value="XM_019163757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTC2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTC2"/>
    <dbReference type="GeneID" id="30180444"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="40"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="504" mass="54599" checksum="D38141EFEFB6839F" modified="2017-01-18" version="1">MVSTLPLISSIELPSGAKYEQPLGLFIDNEFVKPVSTAGKGEKLITVNPSTGKPITEVYCASEKDVDVAVKAANKAMETWKSVEPTVRVELLLKLADLVDEHTQTLAAIEALDSGKPLNSNAMADVESVASYLRYCAGWADKLHGKQIPISSTCMAITKRVPLIVGCIVPWNYPISMASWKFCPALAAGCTIVMKSSEITPLSLLYFAQLVKLAGFPPGVFNVLSGYGKDVGSAISRHPGLGKVAFTGSTLTGKKVMADAANSNLKSVSLECGGKSPLIVFEDADLKECAKWASFGVMYNTGQNCTANSRILVDEKIFDSFLKIFLGQLKKDWKLGNVFSESTTMGPVVSQQQYDRVQSYIKIGVEEGATLHQPLLDTTPSAGFYISPSVFTNVKEDMRIVKEEIFGPVVTISKFSTESEAVKKANDTIYGLAAMIFTTNFERANRVADQVEAGSIYLNSSNNEDVRVPFGGMKMSGIGRELGEEAFNLYTVSKSIYYNYGSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTC6</accession>
    <name>A0A1E3NTC6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_29264</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020417.1">
        <property type="nucleotide sequence ID" value="XM_019162581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTC6"/>
    <dbReference type="STRING" id="763406.A0A1E3NTC6"/>
    <dbReference type="GeneID" id="30179268"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="550"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="651"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="923" mass="104809" checksum="FBCFEA9B55ABC866" modified="2017-01-18" version="1">MHNEDVKRITEGFISDSNVSLQTYLEQLVFVVENNINGIYLLGEIKKTYILLFAVLALQCFLVSNFSGPNLPFENTYDETGFLSLLLTGNEEFNKRLQLQSLSLLSISGLSPYHLTESPVLLVLALKFFEKLQGANISLLDSSNVKLESDALVENSFDSESIRKDDPHSFIVGALCWWRARALQVQQSLLPDVSSELTSLSLLLLSNKTVDSLVNQSTPNSETNQSLLIEYYLEKANISIAGELESQTLEAILNANKVSGLSLILTGCKAKMTKYQQKSTATLTVLAKSHDALLRSEQNEQSFNPQDVKLNDDLFLEMPQYDSLGDSELLNQNESEDNEFIKRIKLDFSSLASFESSLPSTKDKLLPTATNESDIPEKLSDIDCNKQPRLANLDYVQLILRMQAILNNTPSDNALVNEELIAIIQRILFTTENTVNWLVFSRALWYRSLLETARPRTVERGILQLYSLVEELGVTGDKTARLFPKTEDEVKFSEEFKNLTSIEKPLINSIRLRYIYLIPLMPKWSMDSKLAEKLVELGAFKSALEIYERLEKWADVALCYASTGDKARSIMFIKKALDKDPKDARSWSVLGDIESNPEYWNKAWEVGRYANSKRSLAKYYYNPPKDSGVTKDLQKAIDNMFECLTANPINFDNWFFYGCMGLESGNYELAAEAFTRCVSLDDTNSYSWSNLASALIQLDKLSEAFTALQKSVNSGDSAKKSWKIWENYMLVAVKLGKWDDVLYSSIILLNRKKELEHDDSSIDLPVLEKLAELLISEPLDASKRETYYQKTCTQFICEMIPTVVLHDARIWRIVAKVDLWRNKPWLALEDYEKAYRAVTNNPDLSTDENIWKDAVEACTELISAYENFGELEGRHGAGDLVCKDWKFKAKSTVRSLLSKGKSTWEYTDGYEKLQELKAEIMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTD1</accession>
    <name>A0A1E3NTD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14785</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020433.1">
        <property type="nucleotide sequence ID" value="XM_019160531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTD1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTD1"/>
    <dbReference type="GeneID" id="30177218"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000078">
        <property type="entry name" value="MFS maltose transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF83">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="51"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="543" mass="59911" checksum="38FDBB63148E4696" modified="2017-01-18" version="1">MKDTISDEKSAGVQETAIEAALGHDPVTLARVEKEIGVLEAIRTYPLEVFWCAVFCLGVVMAGYDGQIISSFYALPAFQKRFGNLQSDGTYQIAASWQTALGMGSPIGQVIGTLGIAWPLEWYGRKLTYSVVCVGCIGLIFMQFFAPSLGVLTAGEILAGILWGCMVLIGPLYASEVAQIKLRGILTAMSNLSFVIGQFVANGVSDGFASNSTEWAYKIPFAIQWAWPVIILILLPFAPESPYWLVRHERIDDARKAIRQIASKSVSDEIIEERLNLVVDTDKLEREMEQTTSYRDIFKGSNLRRTEICSIVYIIQVLCGVPFAMNYSTYFFELAGFASSRAFDLSLGSTAIGFVATLLTGVSLSYIGRRPLYITGLTFTTILLFIIGFLDIPSNYLSRPSLSQTQAALMLIWSFAYQLTAGALCFVLNGEVPSTKLRSKTIAFATAVQALVFIAATVALPYMLNPENGNLRGKTGFIFAAFSSVCCVWAFFRLPETANRSFEELDIMFHNKVPTRKFANYVIDYSEVKALTFEESESEKNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTP1</accession>
    <name>A0A1E3NTP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020567.1">
        <property type="nucleotide sequence ID" value="XM_019160591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTP1"/>
    <dbReference type="GeneID" id="30177278"/>
    <dbReference type="OrthoDB" id="2285229at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF59">
        <property type="entry name" value="EXORIBONUCLEASE II, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="1">
        <location>
            <begin position="569"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1044" mass="119022" checksum="120E4A1E451E6AD9" modified="2017-01-18" version="1">MLSRRAFLRTRLVTKLIRIPVFAPCTHFSTSSPSLRGPTKYKSKRNKIDLDRRIQTPEELFQQASNVDVENLRKDVKERTEIRYIKPNKFWISRVFPDLTIPPSFSYYDDHSNRNYIKYQLVKKFGDINKKFSWKDLQYTEVDVGDVVDLSGNFEKGDLAVVIELPSSPDDPRYTLVNSYGEIQFVSRSKMGLRIPKVFPKEWLENSVFEEDLFWKDLDTEEIVPVGKPKYKLEDVVDRNKIFESAVSRSISGENAVKTYILPSILSGIISESLTEMVTKAWQFLPDANIKLEVLHNVLQSNEAPIQLSLYQLFNAVELTDLQVLIKDFKSQKPEDINKGYKKLFRSVASTLSIGNQYDSITLGKEITGNVNLGLKVDSTRFYAFILSLRKNNQLYSHDAFSKASPYALVLPLNRIVKFNKLVQSFKQDEKLHEQLAGYITSKLDPNMKEKAKSKEIVSHYEVFIELLKLYCAGSVQNSVLESFVIKIMRMLANYKDLDITSSSVYDLLLNTKEITNTEGPTKWWDNAMIPYSGVSVKCDYEQTYYDSISKDNLNDYIDLNFDNHTKERAQFDDVIYCIDSENPLEIDDGVSIKKANEDEYLVSTFIADPASFLDPSSLVAKIAFERGLTLYLPDLAGINAVSLLPTEFGQAIQLGDYDKDTRVLKITFKYNIRSKQVIELEDKEAVTFGSAKKFVKIDYASVNKILADDKDSSAILESKSQEGVSAQKIKQDLKDLSVVSNHLNRSAAEGGRVSLFDQINVKKEIENISENENKQILLTFKDVYNDDTTANVIGRGAKSEQLVSEIMVMANHLAGNFCEKNNIPAIFKVQLPLVMSPEVTRFSNEITAKADSSFKELTLYQEYLTKSTIAPFAAKHEFLNIDNYATVTSPLRRFWDLVNHWQLHSYICTGRPRFNEEQMNYIALQLKYKDELNSKISNKVLGFYTFKALKFLQGQHTEQGKLKFKVIVTKKPSESGLIEVIMLDYGVRCILETSWYALSDTKTEIEKNKVLAKNIEIGDTIDDATIKDIDLLDGSIVLSSNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTS1</accession>
    <name>A0A1E3NTS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH-quinone oxidoreductase subunit D domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70986</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020552.1">
        <property type="nucleotide sequence ID" value="XM_019163790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTS1"/>
    <dbReference type="GeneID" id="30180477"/>
    <dbReference type="OrthoDB" id="1009at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.645.10:FF:000005">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01962">
        <property type="entry name" value="NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="6">
        <location>
            <begin position="233"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="503" mass="57048" checksum="033C30B7D772B18D" modified="2017-01-18" version="1">MLSATLRSSFKQASHVSRLAQFNRRCLSSTSLVKEMPKLHKIEQEARDNDFYIIKKRGDKGGESAVEGEDGFTSTVNMEINSAMGSYSDFVNDAKDWETWTLENEDSPDHTVKKSKIRHFTLNFGPQHPAAHGVLRLILELHGEEIIRSDPHVGLLHRGTEKLIESKTYMQALPYFDRLDYVSMMTNEQVFAVAVEKLLNIEVPIRAKYIRTLFGEITRILNHLMSVLSHAMDVGALTPFLWGFEEREKLMEFYERVSGARLHSAYVRPGGVSQDIPFGLLDDIYMWATQFGDRIDETEELLTDNRIWKQRTIDVGIVTAEDALAYGLSGVMLRGSGIPYDIRKAQPYDAYDLVDFDVAVGTKGDCYDRYLIRMAEFRQSLRIIEQCLNDMPAGPVKVEDFKISPPSKELMKNDMETLIHHFLLFTKGYQVPQGETYTAIEAPKGEMGVYVVSDGSERPYRCKIRTPGFAHLGAFDHISRGHLLPDAVAIIGTMDLVFGEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU37</accession>
    <name>A0A1E3NU37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur cluster assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14948</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020619.1">
        <property type="nucleotide sequence ID" value="XM_019160610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU37"/>
    <dbReference type="STRING" id="763406.A0A1E3NU37"/>
    <dbReference type="GeneID" id="30177297"/>
    <dbReference type="OrthoDB" id="1925777at2759"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000005">
        <property type="entry name" value="Iron-sulfur cluster assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="9">
        <location>
            <begin position="53"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="187" mass="19856" checksum="5F884DC8E82F52C6" modified="2017-01-18" version="1">MYARQVFAQKIVKGSGSMLRTLARRPAVGAANGNGAFGAVGSTQLQVSQTRLYHPKVIDHYQNPRNVGTMDKSLKDVGTGLVGAPACGDVMRLQIKVNDKTGVIEDVKFKTFGCGSAIASSSYATELVKGMTLDEAASIKNTMIAKELSLPPVKLHCSMLAEDAIKSAIKDYRAKRGRVTLGPDAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU44</accession>
    <name>A0A1E3NU44_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_70759</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020311.1">
        <property type="nucleotide sequence ID" value="XM_019163730.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NU44"/>
    <dbReference type="GeneID" id="30180417"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07470">
        <property type="entry name" value="CYTH-like_mRNA_RTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="18">
        <location>
            <begin position="317"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="16">
        <location>
            <begin position="618"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="17">
        <location>
            <begin position="1028"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ49198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1292" mass="147692" checksum="0D6EA8B7DEA92A2D" modified="2017-01-18" version="1">MDLRNLISRTSDDESSGPNGPQAQLSKRASQPRLSIHSLMNDNDNEKNRANDMDNDKENNVKADTKEEIALPNASQGANKDASNSGPPIPKPTGSTSEHEVPTIEQKPAPFLPSDATGINVDLIEKGEQESMREEPSFQTTNTTEAEEQENFKDDSKHESGDDSARIKSRNSISALTNVSVNETVAHKPPLPDTRLPKQENEVVSGPPSTNDIIGKEDRKAVEEMDSKSQKETPEKSLHEQIQKLEDLKKKEDVEAVADGKPKRYKNKPTWAQDYIPSFNQPASAGSNGYQSFKGDVSLNNISKLSVPSITGSIPRNDFNKLVTEWIWANVEGVKQDYLDIPNVEDYIEIESKVGNIWDKVKDRRIQLPVNTECVVATDYVNQECFFKPGITLENYNDTKSFISKLIQEAAEQQQQGKGDPSNKFIIENSHVVDLIASDSRRGDKPISGRVSLDIKTKRKTNSISKQRISDLYLHFPNTLFDVRMSLSVELPKDLNDAAFEIFKKRVTMEREKERVSYIHQATSTRIDLTKVREKNNRIPKYELELEINTPALLRSMRNVMDDPLYYMDLIYGLIWSFSHKPRAPFTSELSYTTNDDDHKAYKLPRFNSAPKDDIILSVVVPCFNETSRLKIMLQEAISYMEAQHENNKLQKSFEILIVDDGSKDDTSNYAINLAKEFNLPPHTLRVIKFEKNRGKGGAVTHGMQCARGSYIIFADADGASKFSDVSKLLRAIQKLDHNKPLEIPAVAIGSRAHMVNTDAVVKRAFIRNLLMYGLHTLVFIFGIRDIKDTQCGFKLFNKAAAEMIFPNMHTEGWIFDVELLILANRQHIPIEEIAINWHEVDGSKMVLARDSVNMAIDLVVTRMAISQYMKKDIEKVATLFYPLRKLMEGTLKAPSEIPSYRIESGIRHVQPYFQHIKTTVKSRWLNRSVLDVLSTEFRSFSISQYRSRMEKNEISVLHRQKLTKKERKERCKDNLRTDDRIVIRYPEILKYKMADNDIIERLEHIHERSVCATRAQELEVIHEDDEVLVVSKPSGIPIHPVQNYYYNSFVQILQIEGWPGRKEESKDIQLRPCHRLDKLTSGICMFAKSAEAARKIQTKIQDRTVEKTYLARVKGKFPGVVNMGQIDQGEDIKCCDDIVVFDTKKGKNDGVMKKTAMTVFRSLKYNKELDESIVMCWPKTGRTHQIRIHLRNMRHPIVNDPLYGFNRLLCLENIGGDTSNISDEYFQRIEKQAAEKRSEAESGESCHFCEGKLYTQTEKDDLIMYLHAYKYQLDAESGWQYQTKWPEWCNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU48</accession>
    <name>A0A1E3NU48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_104794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020683.1">
        <property type="nucleotide sequence ID" value="XM_019159675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU48"/>
    <dbReference type="GeneID" id="30176362"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="113" mass="13772" checksum="B7DE0D986D05C567" modified="2017-01-18" version="1">MSPLIWVQTHSHSVHIQFVFSFHDQISSIQKFPVFAPRLLHVAALFFIPFIPNEYYYYLQLFLSLHFYSSLFLPFWFFFVYQSIPLYLFLLINHLIRFFPFVFLFFTLPLCLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV71</accession>
    <name>A0A1E3NV71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_61955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020671.1">
        <property type="nucleotide sequence ID" value="XM_019163464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV71"/>
    <dbReference type="GeneID" id="30180151"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133538" evidence="2">
        <location>
            <begin position="22"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="162" mass="18370" checksum="2CCBDAF4AC5D6659" modified="2017-01-18" version="1">MRLSLIHALAFFTAFASECSCNDDSYVASEPRIINFALIKLPEEVKNEIRVPSIMHSWEETEYIMLTNDDTPFEYALNMSYLDLAKLHLLNANSGKFKGNDYAGHQASYKGNLTELTLDDVIYLYYTEDELVLDRSIPFYTRDETKTATGESSPTSKPGSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVD9</accession>
    <name>A0A1E3NVD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_28419</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020626.1">
        <property type="nucleotide sequence ID" value="XM_019162540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVD9"/>
    <dbReference type="STRING" id="763406.A0A1E3NVD9"/>
    <dbReference type="GeneID" id="30179227"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000174">
        <property type="entry name" value="YIR007W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="4">
        <location>
            <begin position="79"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="5">
        <location>
            <begin position="647"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="767" mass="87798" checksum="0DDA0E88CAECEEE6" modified="2017-01-18" version="1">MFPTPLATKPLVVDRNGQFREKDTGRVFRMHGVNFASNTKMPEEPCQNSNMDPEHCGFYERADTVTFVGKPFPLNEGYQHLSRIKQCGFNTIRFVTTWEAIEHEGPGIYDLEYADYLVGFLKLIDEVGGLYVFIDPHQDVWSRFSGGSGAPIWTLYAAGLEPRNFEKTVAAKLHNLAIDPQNFTKMVWATNYNRLASEVMFTLFFTGSVFTPKAKLDEENIQSYLQRHFIEAFAFLVSRIKSEIPHVFDTCFLGIESMNEPNSGFYGYADLHQCPPHQELKLDETPTPIQSLRLGMGFPEEVDMYSVSIFGPSKIGRRWCDPEGTKAWVTENDNKDKHYGFKRSPDWKLGECIFAQHGVWDTITGELIMPDYFKVHPETGEYLDESTFINGPFLDFWLKFKQRMREIDEEMFIIMQPPVLQIPPVIKHNSEYLDDKTVVALHYYDGMSLLFQKWNRMLNVDTLGIMRGRYLNPVFGLVLGESNIRKSIQKQLKSMATESHQNVGYNVPVIFTETGMPFNMDNKKSYEDGNYTSQEAANDAILSALEREQLDFTYWCYNPDNCHDWGDLWNLEDFSIWSKDDLPGLQLQDGDTESTYSHSNSSINKELVREITHSYTDSNGKSSGNDWSSQLNIDLSGGVRAINAIIRPIPLLVNGDVNMCEFDMTSKSFHLEISNARFQSGEPSPSIIVIPEQHYSNNNFRIRVSSGDFELRQNPIMQWVEWNHSESSDKVVVMDVTAIDEYADDVIFKKNDCGSLKAFACGYLSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDR2</accession>
    <name>A0A1E3NDR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18515</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015384.1">
        <property type="nucleotide sequence ID" value="XM_019162035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDR2"/>
    <dbReference type="GeneID" id="30178722"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000180">
        <property type="entry name" value="MFS monosaccharide transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF6">
        <property type="entry name" value="MSTA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="53"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="568" mass="62875" checksum="0656386DB1201535" modified="2017-01-18" version="1">MNFASETKSDGQTEDLSTQYNTVTTVSSSTDVEKQSEFHEREEAPITWKTYVMCGFASFGGIYFGYDSGYISGVMGMNYFIHEFTGKVNDGTDAFVLDAWQKSLITSILSAGTFFGALMAGDSADFIGRKYTVILGCIIFIIGVILQTASASVALLSVGRVIAGLGVGFVSATIVLYMSEISPKKIRGAIVSGYQFCITIGLLLASCVDYGTKNRNDSGSYRIPIALQMLWAIILAVGLLFLPETPRFYVKKDNIEKARASLAFLRSQPEDSRFVSQELDEIIENYRLEQSLIPTQTYMQSWFACFTGGIRNPASNLRKTILGTSLQMMQQWTGINFIFYFGTTFFQELGTIHNEFLISIITTVVNVCSTPLSFYTVEKLGRRTLFIYGAAGMFVCEFIVAIAGTVDGHNPQTVKAMISFICIYIHFFASTWGPGAWVIIGEIFPIQIRSRGVALSTASNWLWNCVIAVITPYMVGEDKGNLGAKVFFIWGSLCGLCLVYSIFLIPETKGLTLEEVDRMLEETTPFTSARWKPHNYTAEQREAIGHVTVGNDVDGDFEDPKPELAHIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDV3</accession>
    <name>A0A1E3NDV3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_22582</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015424.1">
        <property type="nucleotide sequence ID" value="XM_019162269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NDV3"/>
    <dbReference type="GeneID" id="30178956"/>
    <dbReference type="OrthoDB" id="540662at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000393">
        <property type="term" value="P:spliceosomal conformational changes to generate catalytic conformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="158"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="286"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="366" mass="39771" checksum="9C35B513B26A7FC8" modified="2017-01-18" version="1" fragment="single">DDDDEFYTPGPDSLYASRVVIADHSLQSAQRRVQRQYDAHRAFDSVANLLARRDCYAFLRSSLTLQGTQPVSSRFTSAVAHSGPLGVLAVASWDGGCYFVDPSDLAVRSQLPGLHPEKVSGLQWSPTAASILATGGSDGTLNIVANPLDASSAGITPLTGHLARINDVCFHPLGRFVASASADLSWRLWDVETAQELYYQEGHTDSVNTVSIHPDGSLLASAGNDSVVKLWDLRSGKSVMDLHEDGHIKSVHALDWRSNGYHLASGGADAQLLLWDVRMGKKVASVLAHDKLISNVQFSPSGEFLVSTGYDGVLSLTASDSWIVFHKFKTLDKIMACDLFYRNGSNDYKDLNIVTGGWDRSVKLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDW6</accession>
    <name>A0A1E3NDW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015432.1">
        <property type="nucleotide sequence ID" value="XM_019161918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDW6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDW6"/>
    <dbReference type="GeneID" id="30178605"/>
    <dbReference type="OrthoDB" id="251770at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561">
        <property type="entry name" value="DNA REPLICATION REGULATOR DPB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 2-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="3">
        <location>
            <begin position="95"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="3">
        <location>
            <begin position="341"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="540" mass="61998" checksum="5B2B99BE920B92EF" modified="2017-01-18" version="1">MTFQVVVTSTALTEVEAKVLEKALQENFCMVKYSPDLTSEVQVLILNKKDPFKHWLKSKKFMYVTAYRPDIRVIDFDDICNRKGEQPSAILLKIRQVKPFEGLQISLCRLEEGLSEKIHSVVESNGGKVIHHLTNETDVMVSMIAEGKRYEAALKWGVAVVSPDWCYDSVDRGLPLNTRYYKLLQNMAGVVTKLNYEGEEDKTGSETVVKTYRLGRRADACDWEKLKEWRDNEHARKLEDYIKSKIDGTEHNKHNDEAAHKGSSEDTNEENFTLFEENVTDLNSNKRVFSQLDQVDDEDKVVKIKKVGKAGELWNSMMHKEKKINPKFANMKTLQNNTRESKCPILQGLKFKTIGYSSRENEKLAKVIAKFGGKVTTDPEDQVNFTVVNFRYNEIVQGYNLITELAIERFIFNEKVDAGDYLWCKPFFVDQTKPVSAFKEKFFIGNEEIGKDGQDGKVKVSITGFEGTDLSQMERLLKEKLSPWVEFQQIFSQACQLLVVGLSGHTNTGRLRKQQLARRWNIPVFLVEDFYAKVLELSAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDX1</accession>
    <name>A0A1E3NDX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Symplekin/Pta1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18256</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015448.1">
        <property type="nucleotide sequence ID" value="XM_019161925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDX1"/>
    <dbReference type="GeneID" id="30178612"/>
    <dbReference type="OrthoDB" id="331600at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021850">
        <property type="entry name" value="Symplekin/Pta1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032460">
        <property type="entry name" value="Symplekin/Pta1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245:SF20">
        <property type="entry name" value="SYMPLEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245">
        <property type="entry name" value="SYMPLEKIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11935">
        <property type="entry name" value="SYMPK_PTA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Symplekin/Pta1 N-terminal" evidence="5">
        <location>
            <begin position="130"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="370"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="485"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="485"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="800" mass="91677" checksum="91E08AD516CE690A" modified="2017-01-18" version="1">MSSVPSSTLTQTSAAAANGSNAAAVKSSDEDQNLKFQLKQIEEAAKLAFENNDYFVQILDTIVAISLAQPLNNTKLQIKCLNFIHKSFVSKQISSFDLRVQNCLKLVDMLSKFIINSEVAINLEKISYNLIQKSIEIFASVYGLMFLQLTRFPDSQSWSKISKLKDFLISKWPSAYPLLPLNKETDLTRSTGCKIALAKLLGKVIQTQLPCPPNSINPKNDLSAVTDDNMDISISLVNKNHPFLYSSSLNTQGQMLIDYILNLLNNDALLPTSFFSTLIAILMSLFKTRPNFTSSKILGFILAYESQFKNPPKFETDKLKVRLTRRFNDRIDRILMSMLLNRGFIDKDPPLKSRFANKLSYMVEKSNKQKKRSILSDENDIEDEHRSEDENSQKLKRRKLDTEKSIDFFDESRIAKEETYKGIYTLINPKDELANFDISAIPSEFINNIVILGLSKTDTNKLNAGLEIISSRYLDLYNRFQLREQQQQSANNSSDNKLNAQDKQQNKNNEEDDLYDPSSGVVKKEENGNDPDKKDEEFDFELESKNFELPLPKILDPDEKQDQIKLIVDNFIKTSSAKFGNDKDSEANKTDTQELKKVAISRWKRNSWIKLLSRLATRGTKLNPEISNYIRDSLFNYFKEDIKGRIDGVIEWLNEEYYDEFIVSKDESKDMSSSNYMKYTGLVLDYLIPFLEPSDRNIFIRLLSELPYLDLSLISKLRSVCTDPVRSKIGFQPLLYLIMFRPPVFDDCINFLSDLYNYAVERNNGQLQNECLGYLKKYKPTIIPSLEKPPQDNAVTADTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE09</accession>
    <name>A0A1E3NE09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18286</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015484.1">
        <property type="nucleotide sequence ID" value="XM_019161931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE09"/>
    <dbReference type="STRING" id="763406.A0A1E3NE09"/>
    <dbReference type="GeneID" id="30178618"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14723">
        <property type="entry name" value="ZIP_Ppr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF1">
        <property type="entry name" value="PYRIMIDINE PATHWAY REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="14"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="701"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="749"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="872"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="121"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="750"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="781"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="873"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1079" mass="120469" checksum="E0B992E5E9181296" modified="2017-01-18" version="1">MAHQILGITRSISACKRCRKKKIKCSHDFPRCTACLKANTECVSLDPATGREIPRSYIMHLESRVSELENYIQGTLAPPGASPQSQPQQQQPQQQHRFQPDLTASYTNTPSSTTHSNTPSFVSPASYKNITPASTDNTNVPSQSENNDSIIDTIHNYNPSASQDDSSRPTTFSGNGISFSKLMFAAVHFKDTADPKDRERRRSSAAAAGANIKPPIDPTTVVMSSFSKNVALLPPKQQALDFLSLYFAQSNSQLPIFHREEFLERYFIPIYGKIPENSAFASSNSSIDWSRLPEIEESDTWYYQYTRLLDAETGVDPEMDPFKFSAKVSVPRRFRKALYFTNIVFAVASSIWHLQFPGEISENFKNAALLHIEDAYSSTNRLEALQAMLCLTLYSLMRPCVPGVWYLSGSSLRLCIDLGLHNESSSSSAEPTTIDMRRRLFWCCYSLDRQICVYLGRPFGIPEESIKVPFPSILDDSMINNESTMNSLDPKNPSEISSYKHIALTMFKIRQLQAEVQSILYDKREIPRIFPSLSAWLADISARLNDWRAGIPQTTKKMNCEFTTEFFDLNYHHARMMLHGLAPARYVLATNDFIKLAEASKETLFTFYKLWNHGVLNYTWAVTQNVFMAHMNFLYAVFHNNEVKNATSLNEIKKLSGYASVTLKSLINRCNAAEQCLEVLSILSQAVIKLKYPVSSTIHIDESREDTPASSTMNRLPSEEEVKTLQPGGHMTGNMRRLIVSIPELINAEENNKPTHAREENTDEEDPCAQRKKPKLLKGGSTDSPSIVDTTTSTPTANVGSLALNQEKTSLQSYNTNADNLWGLSDTDLERFFDEVRKVDSPDSSRSYSHFSSTSNAADDVVASDFREKGANTVTHSPSVSASVSGSISTASPAVSSGSNRSISDGRPIQDDSASFIPGTPQPLSKMPYKVGATHQHYSAQTHVQQDPHFFPGPGSQGQKHQLYPFYHNQSSSPGNVYPSNFSYVSAQNYQPHFQSAPTSFSPPGSLPAFPHPVTSAMNSPARQDFRNTDISPYPLTLNSDSSKRNTKEGQRVYKLMVETGTESIWDQFFAQPFKLDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEM0</accession>
    <name>A0A1E3NEM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_173556</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015682.1">
        <property type="nucleotide sequence ID" value="XM_019161560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEM0"/>
    <dbReference type="GeneID" id="30178247"/>
    <dbReference type="OrthoDB" id="10603729at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="153" mass="17155" checksum="FF3CB7795D6291E3" modified="2017-01-18" version="1">MEPSKRHAGPSLAVRISRLRHQHPALKDSVRRCAVPLIAVVFLLVNYYYNKHVQSRGYPQLGYPLSEHTVDEFHRAVSSKQADRADTPFNAATASIIETISTLPPKVQSIYGPLEFQPTDLFEPFNANSLLFVSGMFVVYKRTPVNPPTAYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEM7</accession>
    <name>A0A1E3NEM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="4">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_60277</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="4"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015708.1">
        <property type="nucleotide sequence ID" value="XM_019163423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEM7"/>
    <dbReference type="STRING" id="763406.A0A1E3NEM7"/>
    <dbReference type="GeneID" id="30180110"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="9">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="314" mass="34653" checksum="8509171613BA6CFB" modified="2017-01-18" version="1">MAAAAKISTKTLKLNTGATIPVIGLGTWQSPDEECYSAVLSALHAGYRHIDTARIYQNEEAVGKAVAQYLKESGTSRDKLFITTKLWCSEVQDPEKALKESLERLNLEYVDLYLMHWPVSMVNRGDLFPKTASGTTEIIPFDKWNYVETYKIMQKLYKAGLTKAIGISNFNIPKIDYLLAQSGVDVVPACLQVEIHPYLPQFDLVDYCTKKGILVECYSPLGSTGAPLLKDPALIKLGEKYGVSPACIAISWAVARDTIPLPKSVHEERIVSNLKVVDLADEDISLVDAVSKTTAKRLVKPEWGVDIFGDSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NER3</accession>
    <name>A0A1E3NER3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Urea active transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_36664</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015727.1">
        <property type="nucleotide sequence ID" value="XM_019163023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NER3"/>
    <dbReference type="STRING" id="763406.A0A1E3NER3"/>
    <dbReference type="GeneID" id="30179710"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="575"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="609"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="694" mass="75420" checksum="AAADABEB671AC093" modified="2017-01-18" version="1">MAIDSQTQFFSPSVGWGIIIGVGAAFALGMLTLSWLLAKYNNEIQDSEMLSTAKRSIKTGLIASAVVSSWTIGSTLLLSCTDTYLLGHSAAWWYGAGACVQIIIFAVAAIELKRKAPNAHTFQEIVRVRYGKAAHLTMCAYSFGQQLFYTANLLVNGASVFSNITGVSKEGSTVLLPLFVIIYTLIGGIKATFLTDWTHVVIIYVIMLMFLFKAYTTSPLIGSPDKMYDLLVEAGQKYPVMGNWQGSLLTFRSINGGLFGLILFGAGWAAAVDSQLFQKAIAANPAAALTGYTLGGLCWFTLPYALATTMGLACRALEITPEFPTYPNRLSAEQLNSGLVLPISAYTLMGKGGTAAVLVMVFMACTAAYSSETVAVSSLWTYDIYKAYINPQATGKRLVMVTHAAVVVFSVCAIVLAIGLGQANFDVSFITTCIGIIVNVCIIPMGCTLFWRRMSALGMILGTTVSTGISIAVWIGYAKAQSGTVTLAALSTYEALAAGNTVAVGVPAIVVPIVVYFKPANFDWNKWKTDIHQDDNSEFDRAHGLTNVLSGEELTELVLEKERAEDALMIRRRNLGYVIVVVFVLFFLILFPIPLYGTKYIFSKTFFRGYIVVTFLWVFFSAGVVTVYPVWESRRGIANLFRKIIHGETTPQMYSDDTLSRVQTLRSRASAPDRSSADADVVVKNDVVVKLESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF21</accession>
    <name>A0A1E3NF21_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_18394</name>
        <name evidence="15" type="ORF">PICMEDRAFT_60369</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015610.1">
        <property type="nucleotide sequence ID" value="XM_019161985.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015832.1">
        <property type="nucleotide sequence ID" value="XM_019163430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF21"/>
    <dbReference type="STRING" id="763406.A0A1E3NF21"/>
    <dbReference type="GeneID" id="30178672"/>
    <dbReference type="GeneID" id="30180117"/>
    <dbReference type="OrthoDB" id="3993282at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000007">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="13">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="103" mass="11407" checksum="C11136D02509CA39" modified="2017-01-18" version="1">MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISALIYEEVRAVLKTFLENVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFS5</accession>
    <name>A0A1E3NFS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ran GTPase-activating protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73787</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016100.1">
        <property type="nucleotide sequence ID" value="XM_019164587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFS5"/>
    <dbReference type="STRING" id="763406.A0A1E3NFS5"/>
    <dbReference type="GeneID" id="30181274"/>
    <dbReference type="OrthoDB" id="184583at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006404">
        <property type="term" value="P:RNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="346"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="44738" checksum="C950BA02806D72F5" modified="2017-01-18" version="1">MTTIHNRDLGIQDSHTFSLKNKGIKLDTAADIKPYLEQLSKLENVAKVDISGNTISPECSGLLAEALLPFKDTLKDVNLQDIYTSRDRFEIPASLAKFFPVLLELPHLSVLNLSDNALGQDAIEVLEDFISKCKTVEYFIISNNGLGPFSGARVGKALYKSAKLREAHPESSKPLKAFWCGRNRLENGSCEALSVGFRANRELEEIRLYQNGIRPSGISKLISFGLNSLANLKILDLQDNTFTLPGSYALSENIANWPQLIELNVNDCLLKATGCKLFLGKLAEISDKSKLQILRLQYNELEADSLEIIAQFLKNMSDLAELELNGNRFEEDSELIEKINDIFEEKGLGELDELDDLEEPDSDEEDEDDEDDDDKEEAEPETEVDARLKELEKELSAAHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFZ3</accession>
    <name>A0A1E3NFZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17558</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016170.1">
        <property type="nucleotide sequence ID" value="XM_019161645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFZ3"/>
    <dbReference type="GeneID" id="30178332"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062137">
        <property type="term" value="C:cargo receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="275"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="368" mass="41734" checksum="1A5C11291A094190" modified="2017-01-18" version="1">MLSFRTTGYNGYSCKYSPFYDNKLAVATAANYGLVGNGRLYILTIGNDGRISQDTYFDTQDGLFDVAWSELHENQILTCSGDGSISLFDITLPKFPIKRFHEHQREVFSVYWNMVDKSIFCSSSWDSTIKVWSPVRDQSLLTLTSNKDMTAQTENIPSGDKPPLSTKPVAENPVNDCVYQATFSPHDPSIIISVNSASHCQVWDTRAPTPLTLDFISHAGLEVLSCDYNKYRPTVIATASVDKSIKIWDLRMIPDLQRNFLAQKNRVGPSPINKLIGHDFAVRKVQWSPHSSDTLLSCSYDMTCKVWKDQTDENAKYMSRLRMKHGEALVGNFDRHREFVLGCDWSLWGRGFVASTGWDEMLYVWKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG07</accession>
    <name>A0A1E3NG07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73871</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016198.1">
        <property type="nucleotide sequence ID" value="XM_019164614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG07"/>
    <dbReference type="STRING" id="763406.A0A1E3NG07"/>
    <dbReference type="GeneID" id="30181301"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="7">
        <location>
            <begin position="175"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="499" mass="55958" checksum="771AFF8AE7426338" modified="2017-01-18" version="1">MDRDGEGLAESMFSPGGSGPAQTEAQKEAEKRQQILDEYNMKALSQVVNENELRPVNILRLNVLGGEGKFRDSFLKRQLEPICSQLDDGGQTLTVSKLLKRIDLVNMNLVKTDAISNMGCQLAVPERQPYNVFNPDLLNLVVNMQIVPVKKFFMKIGTNVGNGEGDGYVKLQWKNIFGGGESLDLDTNISSDEFRLKSSRSEYLINYSCPLFNLPDYKFSSVFYHSSRSIDYTSFHNQAIEGLTLKLSTNRLPMENKLNHELSFENLVRSVDLRSSPNASYRNNSLITDYFLFNSGHSFKSSITYSIVRDCRSSRYIFDSGYYMRMSNELSLFSQNKFVKTCFDYSKAHRLSKDMVFNLNLKTGLIHPLANDLVHPMDKFQLGGANDVKGWQVSGMGPKQMNMSVGGNYFHALGLNLFTSVPYFTDSNFKLHFFSNVGKLTNLANAGNILRNNGVSAGFGLSYSHPMAAFELNWVVPISANANDGLRKGLQWGIGVSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG34</accession>
    <name>A0A1E3NG34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HCP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_20010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016185.1">
        <property type="nucleotide sequence ID" value="XM_019162150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG34"/>
    <dbReference type="STRING" id="763406.A0A1E3NG34"/>
    <dbReference type="GeneID" id="30178837"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATOR SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="37883" checksum="7E816AE9F0C8AA54" modified="2017-01-18" version="1" fragment="single">LLPMGTSIDMYRQNAKKTKDPDILFSFAQILIRTSLSNRSDNPLSEKEKEKYLDEAFTALKKSSKSGCIEAQYYLGDAFSVGLFSKGKPELNKSLTYFESAAKAKHAESAYRTAMCYKKGWGCTRDARKVVKYLEIAAINNHPVAMMEYGIYAFHGLMSFPEDVNTKKKGISWLRRATECATEMSCGAPYELALIYMNGFKDIVIKDTNYAVKLLFQASNLGHAKSATMLGKFYEIGDIVEANADLSIHFYNMSANLGDADGMMGLCSWYFVGSEHLPQDYDEAFAWAIRAAENYKHNKAMLLLERFYELGVGCEKDMQKSKYWGDLARKQEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGT8</accession>
    <name>A0A1E3NGT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Cytochrome c oxidase subunit 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Cytochrome c oxidase polypeptide VIIA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_17842</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the fungal cytochrome c oxidase subunit 7a family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016483.1">
        <property type="nucleotide sequence ID" value="XM_019161772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGT8"/>
    <dbReference type="STRING" id="763406.A0A1E3NGT8"/>
    <dbReference type="GeneID" id="30178459"/>
    <dbReference type="OrthoDB" id="2317211at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22888">
        <property type="entry name" value="CcO_VIIa_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014368">
        <property type="entry name" value="Cyt_c_oxidase_su7a_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28264:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28264">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000283">
        <property type="entry name" value="COX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="60" mass="6848" checksum="7A8B808991BA3B9D" modified="2017-01-18" version="1">MAIAPITGFMKRRIIVDISMGFAIGAVMGAFWWRQHKQMIATRENYYATLAEKKKIEDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH01</accession>
    <name>A0A1E3NH01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AmmeMemoRadiSam system protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016515.1">
        <property type="nucleotide sequence ID" value="XM_019161788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH01"/>
    <dbReference type="STRING" id="763406.A0A1E3NH01"/>
    <dbReference type="GeneID" id="30178475"/>
    <dbReference type="OrthoDB" id="417112at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35330" checksum="200105854FA70C89" modified="2017-01-18" version="1">MTKPVIRSASHAGTWYTSNPETLKTQIGRFLNSATERVPGARVVVGPHAGYSYSGPILGKTYGTLDPTGIERVFIMGPSHHVYFKGCVLTTDCDYYDTPLGKLEVDKDVINELIEDDPKLFKKMSLEVDEDEHSFELHMPFLRKVTNDLGIHPKIIPIMISATDENFEKRVAKSLKPYFEDKSNAFIVSTDFCHWGSRFSYIAYTPTGTLTDIEDISKSKHPSNSQLPIYKSIEFLDRAAMKTISTGSYRSFKDYMYLTDNTICGAKPLGVLMLMMQDSISETSKHSLKWNGYAQSSSVVSSRDSSVSYASGYAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH38</accession>
    <name>A0A1E3NH38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_17894</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016542.1">
        <property type="nucleotide sequence ID" value="XM_019161797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH38"/>
    <dbReference type="STRING" id="763406.A0A1E3NH38"/>
    <dbReference type="GeneID" id="30178484"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="220"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="45"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="547" mass="63624" checksum="B9F0DA49D847D260" modified="2017-01-18" version="1">MLAFYKKKKNTEVFHFSNFSQETGDYDNENSLKYVEVDDGYNNKSVLESKVPVESSDDELSETSEGEDDKEEEKEEEDDEEVEESKEVDKANDEDEEEDEKDEKDEKDEDEAEEDGDSDDDDDNNEEESGDLGKNKKDSDNTADDNDENNRIEVGKSKSFNYKTQKVNGVNLGGWLVTEPFISPSLYEQASSDGTEAKTPVDEYTFCQQLGPRVARERLKKHWDTWITEADIVKIKSYGFNAVRLPIGYWAFARLPDDPYVFGQEFYLDKTIQWCRTHGLKLWIDLHGIPGSQNGFDNSGRRDVYMWANPQNYNLGIQVLQYIYDKYGGEKYADVVIGYQNLNEPLHTRYEVPMIVEFDRTTYNMFREKSDNYFVFHDAFLPPEFWHRQIDEPQFKDTVLDHHRYEVFHTTQLKETVDGHITSIRLITEKFLNVPRVQVVGEWSAAMTDCAKWLNGVGRGARLDNTFEGNGVIGQCRFSNDYSKMTEQDKIDTRRIVEAQLDIFNQTNGFFFWTWKTENAIEWSMSSLAEHGLFPVPLTDRKYPRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH68</accession>
    <name>A0A1E3NH68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_65103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016603.1">
        <property type="nucleotide sequence ID" value="XM_019163485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH68"/>
    <dbReference type="STRING" id="763406.A0A1E3NH68"/>
    <dbReference type="GeneID" id="30180172"/>
    <dbReference type="OrthoDB" id="73846at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="424" mass="47914" checksum="697954F9AEC3A8FE" modified="2017-01-18" version="1">MTPEEFKSLNSKNTDSFKVAIDMTIHVVPLSPTARFLATQLSSESFRPNILLPKHLLKPFLTSESTITYINEISPLNSFQRKHTYPTSSTRYLETISRISAAATSQISIALPSSQIRPIFNSESIKFIPPPNSDIIILDPHFGQAEKYVSSVMSNFQISASSPYDLRSLPRFWSIVSTHLLADAPSWGIKHQSVGEFIVSPILPFANDSRTEIELINSSDVMKQILNTPTLSPQYEPYLKTKLLLLEQLIIEAALCSIIYTDYSSKYEVLRSHSKSRSFASNNDIYSDVEDSVSAKSLVLNIIDECIAVLRKHEPVLELEKTTPMLTAVLSRERLFNLTLNILQNDTSLPNLKHYKQLMNESFSNYTNDDQQKTESPHFRNPARANRFIFKLGLQVGVKTPSNNFVCDNLSAQYPEIHIHDDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHB7</accession>
    <name>A0A1E3NHB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_59201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016634.1">
        <property type="nucleotide sequence ID" value="XM_019163349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHB7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHB7"/>
    <dbReference type="GeneID" id="30180036"/>
    <dbReference type="OrthoDB" id="408702at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047974">
        <property type="term" value="F:guanosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF161">
        <property type="entry name" value="CMP_DCMP-TYPE DEAMINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="169" mass="18889" checksum="94184D7AC15C6F84" modified="2017-01-18" version="1">MFPQRSTPTKAQIKANLLKAVAAAHTAMEEGRHPFGSVLVGPDNESVVLEQGNVNTLNHAESTLCRVAFEKYSQDYLWQCTLYTNFEPCCMCAGSIYWSNIGRVVYGLSEHALLELTGDHEENMTLDMPCRKIFSSGQKNVEVVGPFADTESDNELKEAILKDHVSFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHI8</accession>
    <name>A0A1E3NHI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">vesicle-fusing ATPase</fullName>
            <ecNumber evidence="3">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_73103</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016702.1">
        <property type="nucleotide sequence ID" value="XM_019164380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHI8"/>
    <dbReference type="GeneID" id="30181067"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990621">
        <property type="term" value="C:ESCRT IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090148">
        <property type="term" value="P:membrane fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02678">
        <property type="entry name" value="MIT_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000004">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045253">
        <property type="entry name" value="VPS4_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIT" evidence="15">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="167"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="443" mass="49321" checksum="C73382F53B75A2AC" modified="2017-01-18" version="1">MSNTSEFLDKGVELVKKAIEADSASRYEEAYQLYYNGLDYLMLALKYEKSQKAKETIKAKFTEYLSRAEELKEHVVNNNQKDQNGEVSSTSGNVKAKKLDPNSSSNNKEEDTDNKKLRGQLANSILSEKPDVKWSDIAGLDAAKDALKEAVILPVKFPHLFKGKRKPVSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVSKWMGESERLVKQLFSMAREQKPSIIFIDEVDALCGPRGEGESEASRRIKTELLVQMNGVGNDADGVLVLGATNIPWQLDSAIRRRFERRIYIALPDPEARLEMFKLNIGETPCTLTPQDYHTLANITEGYSGHDIAVVVKDALMQPIRKIQSATHFKKIDTINEETGESLAQYQPCSPGEPSGIEMNWMELEGDQLKEPDLCLTDFLKAVKTNKPTVNQQDLEKFVEFTNDFGSEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHL7</accession>
    <name>A0A1E3NHL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Cysteine dioxygenase</fullName>
            <ecNumber evidence="2 9">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_27541</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 9">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015946.1">
        <property type="nucleotide sequence ID" value="XM_019162499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHL7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHL7"/>
    <dbReference type="GeneID" id="30179186"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0883">Thioether bond</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="7">
        <location>
            <begin position="62"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="120" mass="14019" checksum="0052E056625E8154" modified="2017-01-18" version="1" fragment="single">LLNTYCSVEQDWTHFAQEHSDLDTKYTRNNLFSLPDNLGQVLLLTWKPNSGSKVHNHPQSECWVKLLGGQMEEKIYDASQKKIGMRRILRDEVTHINDSMGMHSMHNRSLKNYAFSLHVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI32</accession>
    <name>A0A1E3NI32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="2 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73284</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016894.1">
        <property type="nucleotide sequence ID" value="XM_019164435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI32"/>
    <dbReference type="GeneID" id="30181122"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="451" mass="51894" checksum="226E095CC4457C05" modified="2017-01-18" version="1">MANSTSDINLESLSQWTEEFKTDLKTQFGGSVLQHANADDVLINRDQEIANKNFFNHKIEIEGLPVMDQKASGRCWLFASTNMLRVGAMKKYNLKEVQLSPSFLFFHDKLERANYFLQMIIDTYKEPAESRLVQWFLLDPAQDGGQFTMMTQIVDKYGIVPDQIYPDSYNTTNTRVMNRMLNFKLREFAMTLREALAAGKDVSIQRKEMQREVYRLLTMFLGNPPKPTEEFTWEFYDKDGKYQSIKTTPLKYAADILEFNTPDYVSLLNDPRNDYNKLVKVDRLGNVANGDAVVYLNLEAEKLAQAVVHRIKSNKPVFFGCDTPKFMDKKRGIIDVDLWDFQLLGYDVDSMSKKERVVYGHSLMTHAMLITAVHVDDAGKPVRYRVENSWGSKSGQDGYYVMSQKFFEEYVYQVVVEKNELAALDVDAKVLDSTDYIVLPPYDPMGALAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIG4</accession>
    <name>A0A1E3NIG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein DML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein dml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_17162</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the partitioning of the mitochondrial organelle and mitochondrial DNA (mtDNA) inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the misato family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017017.1">
        <property type="nucleotide sequence ID" value="XM_019161491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIG4"/>
    <dbReference type="GeneID" id="30178178"/>
    <dbReference type="OrthoDB" id="271881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="8">
        <location>
            <begin position="123"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="484" mass="54789" checksum="EDBE57166808171C" modified="2017-01-18" version="1">MPQTITLSFSQVANQLTTHFNNFQEDRFYKDAANYDNYTHIVPKSIANSKTISNLYPRSVLFDYANGSGALDPKAGFQPDPAPSLALYEYEKIQTAPQITPNAYQKALTMGETPDVDEKVQVDEERTDYWSDFSRLTYNPSLLLTHPDFNYDVETGEGIGKNILTNKFQSHQLGIDSFHSVEKFKDCVDDSIRKLFEEANNVSQVNAVVELDSAWSGVCSETLRYVIEDQLDGKGSKVMIWSLQRDGQYVSSCNNSNKLDRIRKLLSLVMSEAGGIVSLNLDSDIPLLQNKNSVWEKTAFLSLPFDFFNSIKDNDIGGILHQLTDGGLRRFINEVKLDWSPETVLDLGCKDIFSPSKADKRDPHVFSRSVVASMDSDGCRVGKFSNFEQLVETGTPRIYLQTSKSRYSYEFVDTLPAAMKEAKIDAVSLGVTSSLKNNFTDMHDFVSRFCRTDEREELKDNLDNLREAYTFGYELSDEEEDDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIN7</accession>
    <name>A0A1E3NIN7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAR2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_12451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017125.1">
        <property type="nucleotide sequence ID" value="XM_019159927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIN7"/>
    <dbReference type="GeneID" id="30176614"/>
    <dbReference type="OrthoDB" id="3989964at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033647">
        <property type="entry name" value="Aar2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20981">
        <property type="entry name" value="AAR2_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAR2 N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="195" mass="21393" checksum="FDBD558227303EC6" modified="2017-01-18" version="1">MDGSTLYFDDAVPPHCSVGVDLFFFCSEPGRELRGVRNLPAGVHVVHLTSGLHNTRTAQLLELDGRSGYAVRFCHRTGDDAPYTEIHTVRLDRHSVVPDTLGRRVVDYGALAVAGADWRALRFDRSVLGFVRSPDGVLRTHDSTGPELRRRRRRRLAPARARARAAAERARLHHQRQAAAGASGPDGGGPHRGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ98</accession>
    <name>A0A1E3NJ98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_172189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016536.1">
        <property type="nucleotide sequence ID" value="XM_019161515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ98"/>
    <dbReference type="GeneID" id="30178202"/>
    <dbReference type="OrthoDB" id="10595918at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="187" mass="21909" checksum="5A4B6B945D3D425D" modified="2017-01-18" version="1">MNQDNRHFRTRQLWTRNDIEFFSAETGFDQDDDCDCECDKRLSPAHTQGLVSCNNMNRNVLYQVSPAQVERMKMYRAVRLPSSSRIRNYELVKRLEAVAASHSTSVPVVIRAWGKERGYVSWHSEDEEVFDDEEDYDYKEEEEEEYGYYRDADADTEVVDMLDTLQIAVRLSRAEVMLLNKTLTGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJA8</accession>
    <name>A0A1E3NJA8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_59701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017269.1">
        <property type="nucleotide sequence ID" value="XM_019163381.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NJA8"/>
    <dbReference type="GeneID" id="30180068"/>
    <dbReference type="OrthoDB" id="200660at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF0">
        <property type="entry name" value="PRECOCIOUS DISSOCIATION OF SISTERS 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133400" description="Sister chromatid cohesion protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1238"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1250"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1273"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1288"/>
            <end position="1297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1304" mass="149399" checksum="0A26D543367D969E" modified="2017-01-18" version="1">MVGSAVLVSILYNSTAWRLAFAEEIISTAAQPIPTKELLTRLNDLHKELSTLEQDHVDLISLEQVKDDLNNKKLLKNTNKGVQIYVCCCLADILRLYAPDAPYDEVSLTSIFKLFLLQFSNLDDPESPFYEDYVYLIERVAEVKLIALMTDLSSKDKMITQIFENFYALSANKSFDNDSLQQILIDILNEVISEVSQLNLKVLKLILNKFLTNSKNLRNQSNIKVPGFDISLALCEMNSDKLSRFVTFFFSEMILEATKDNGSDLDLDSEDDDSDDASGKKLANFDMIQMTKIHNLAVELWRYVPEILTSVLGLLDNELEADDVRIRMIATSTVSKILALQPSRVNFPTVYLSPYMNWLKKPLDISVEIRICWLGGLVDILEYRNDITSDVVNGLLKTMIDSNDKVRLNTIMSLSKLKPSTFLQSVVNGTLTDTLFKLLREKSVPIRDEVLRLLAALYNYSTGYDQYIKLEFIRKIPDNILKLVYINDNLINSEVDLALFEKLVPFNSDSSVRVNRLLRMLSLLSEKSKAAFFAIIKRQTQFSQVIQQILVLLDDETGDEDVEEQINNAIKWLSKSFPASHNSEFCLHHFIELKNKRFLRLLGLCSMETSDYDTIVNSMKELLNRIKDPKYFDNAIDKNALNLNDMYDTMKLLLLRCSNIFYNVSNLTCLVDINNDQSNDLNQMSKVAINGISEVCSNVLESHVISLIDAIVSRVGTISLDESRLTNDCWKDLKIIFNFTTQNKIETKFSSKFYDTLYDLSLCGTVLEAKYSIKIINNASGSTKGRLFTGLVHKIWPLKQNSPNFNTHLSTLATLFSCDSISVDRIKEDLSKFLASEVLLKNRNKEIPAPDSEVNSEIDERQTWITDEELYYSKENAECLSKILSMKLLTNWLLNIKDTVSDDIELISKPILSMLSSYINRGGEIVATEDTPVSFCSRLRLHAGIQLLKIDQVPAYDDFIDQRRINRLILLIQDVESQVRYLFLNKLKKRLTQNKISRRFLALIFFIAHEPDSSLRSDTTTWIRASFAKQNTKANSKNSLVFEKSYIRLLYMLSTHPEFRELYMAYKGKENNADVENTVIGSEPNSESESELLRKFKEVSTFALTYIVFSLSLIMTSDNISLLYYLSQRIKQFEANSKIEGADLYPDSLYLISEFSQAAINFIGKIRNWNISIWPGKLILPADLFTKLDNESANNIIRTTYILDGLSDSVNEIIRNRWRNEQGVNFRKSKKKDVARIISPEPENPESRSIGKKSLKRVRNHSEEDNNSSDEYEGPKTNKTKENLATNRIRRSRRGRRVNYNEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJE9</accession>
    <name>A0A1E3NJE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17940</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016586.1">
        <property type="nucleotide sequence ID" value="XM_019161817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJE9"/>
    <dbReference type="GeneID" id="30178504"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF165">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="44"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="459" mass="52002" checksum="A85674A6F652DE20" modified="2017-01-18" version="1">MAATTFDTTTNLNNNLSHLSNDFKSSLDNYLQYQKGLALLNNRYSFVSNLQTGSFGKVTCALDVKLDQKVAIKALKKSVSGVSLMANHEVSIMRSLGYHRNITQLIDFFETKKYYVLVLEFADSGDLYDAIHNKTNLGINFQTNHLAFADLCSQLYEVISYAHSKGVYHRDIKPENILLMNDGTIKLCDWGLSTKILKSNDYNVGTEKYMAPEALAKSDPNDYYLSDLVDVYSIGITLLFTLFNKCPFRKALNADPNYSSFLKSKSFIYDFFTSINSVSFRGIVDLLMIERDLNGLKFLVENGMKNGFTIDQEYSISFSKQQNNYINSIVDSFNDTKIENESNENEVFLFDDYDTLNEDDFINEHVLNECNINELNSGSKSNSNYVHSSNVNPISIPIPKFTSNTSSNIAGSMMNSSSTYNPSSLFDSNVQNHNSFINSFEMTNDAWSEQISDLRFKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJJ7</accession>
    <name>A0A1E3NJJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017430.1">
        <property type="nucleotide sequence ID" value="XM_019164188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJJ7"/>
    <dbReference type="GeneID" id="30180875"/>
    <dbReference type="OrthoDB" id="3995925at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133405" evidence="3">
        <location>
            <begin position="19"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="493" mass="54568" checksum="E9589D2A5B1B7F8B" modified="2017-01-18" version="1">MKFLNYLFLSEFIIPALSLIIAKQPNELGSANRDELSHATAHGIFKSILNNREFGSTFVKFSEWESPSGEIYVVPSTEEDADIASAALGLGYRFIKNDNTVVQSSDISNVVKIEEKNISAAGFPFGWLKHDDSPTADDFFPFGSKKFMMIGVNKSSADEQGTEKSVSKEEAEEDDEDDDGGTSSGNENISNFGLFHWFEDEDDCNADGKTNKLFDTDHFLFVGVGSIDVEPKIEKRDLETSTLILTIYTTTTVTSVHGVTITQTADATTTHNEKWVPTPTTDSHNDEIDGHYTEGGNDESSQPATSTDHEPFTFTTPVTTHEQWENPHTESDVVSTTEHEILTNTWTSDIITSTHNVEEKPTETLADYTNKTSVWTSPESWNSYPFSTTETEHVEEPTTLHLTFENTTIPVTSTFAHPSSRFSSFKQFHTTSSFNNTLSSKSSKGSDESIYTRTKVTNASRMTTSENLAHSQGGYWASLFGLILAVFSGVILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJN2</accession>
    <name>A0A1E3NJN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nascent polypeptide-associated complex subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Alpha-NAC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_34047</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. EGD2 may also be involved in transcription regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017434.1">
        <property type="nucleotide sequence ID" value="XM_019162861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJN2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJN2"/>
    <dbReference type="GeneID" id="30179548"/>
    <dbReference type="OrthoDB" id="3169036at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006613">
        <property type="term" value="P:cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14278">
        <property type="entry name" value="UBA_NAC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015901">
        <property type="entry name" value="NAC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="9">
        <location>
            <begin position="16"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="175" mass="19006" checksum="589F35D27F0EEE2B" modified="2017-01-18" version="1">MSIEEIPQGAEVTIVPKAEKKAKDALLKLGLKPLKEISRVTFRKKNNTILAIDKPEVYRTLGGSYIIFGEAKVEDLNKRYQEAMAAQQAAQEVNDAVNTPKDDLAASIQDDLKKASLDDKPAEADAEDGEPADATGLEESDISVIMEQTNASRNKAIKALKEHNGDIVNAIMSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJN9</accession>
    <name>A0A1E3NJN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017443.1">
        <property type="nucleotide sequence ID" value="XM_019161131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJN9"/>
    <dbReference type="GeneID" id="30177818"/>
    <dbReference type="OrthoDB" id="5835136at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="1">
        <location>
            <begin position="33"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="312" mass="36466" checksum="07F8FC4418A86A3C" modified="2017-01-18" version="1">MSHYKIFAWGDGKGIAPFDVSSTILNYLINERTLNETFEIVPNSNIFNSHSGRLPILIGPESFQIEGFAEIWNFIVCSSQTGDEKVERDNPALQILRRAYMDEITRDLNTITLYNYFVVKRNYEGFTRGSFKEYLPWPTQYKPPLDFRHHAQELCLNEGIIDDDLVNPKYNFMAPDLEDELQQLRKEEKNLRETPVINDMQKLEIDKQLDIISQKKCIIANMQCIKKLKEIIQKHEEIMPTLDDKIIHTILLIYLRRNIESQLPENFISIWLKREYPELNEEVSELNLSESMPESNKISLTTALGSYVSQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJW0</accession>
    <name>A0A1E3NJW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA polymerase II-associated protein RBA50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_12092</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RPAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016746.1">
        <property type="nucleotide sequence ID" value="XM_019159879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJW0"/>
    <dbReference type="GeneID" id="30176566"/>
    <dbReference type="OrthoDB" id="348201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039913">
        <property type="entry name" value="RPAP1/Rba50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013929">
        <property type="entry name" value="RPAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013930">
        <property type="entry name" value="RPAP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483:SF18">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08620">
        <property type="entry name" value="RPAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08621">
        <property type="entry name" value="RPAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RPAP1 N-terminal" evidence="4">
        <location>
            <begin position="150"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="RPAP1 C-terminal" evidence="3">
        <location>
            <begin position="434"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="65794" checksum="4D1F816311763CA1" modified="2017-01-18" version="1">MNRTGSDEEELEPAQQEEEAKGRGKETKEVDRAVAAAAGEVASAQMDLLGEIVEHETVVPEAPVGPTPPGQAGITRGFPTARAKRPSRWRQRLQEKGLSSMSGSVGSGGVAEAAPESDGKIYTTAPDTGLKHFKSKGDRKLDYSGLSEVEQIHQENIEILAHMSVAEREDAKQELLDNLDPKVLQMLMRRSVHKYGPPPAAGSEPAAETKDPLYEPVEGSMGTWVGGEHELDRQSRQQFQRGEEKRSTSNTSLKSALKTPLTTPHNSTLEGDAADDGENEARKEKTKRIRFSKEAKVIYLDQKEKVRHSSPSDSDGEWEDFEEVSEDANLRPAVANGASRVAVDDDAPVPIEDAVRLKRSPNDELADPSSSSGVHFPQPTQPYEVLDINDPQFNEKLHEKYFPDLPRNPKQLEWMKSDSPGDIPPVISYDSLESVRFDFKADLITSENIGQYINENQGLYNHAQNPELPGYTIPELAHYLRSTFPGQVCIACRTLGRLLYKLGTLQYQVHEIGDDEKINETGEEGMFEIECWKLIIKLKIIDLLQMYASDKEKNLSVKNYAIDSLWLWKQGNGDEKIKKYASQSEQEQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJX8</accession>
    <name>A0A1E3NJX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16 17">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="6 16 17">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_58738</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16 17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16 17">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16 17">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6 16 17"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017534.1">
        <property type="nucleotide sequence ID" value="XM_019163316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJX8"/>
    <dbReference type="GeneID" id="30180003"/>
    <dbReference type="OrthoDB" id="10260889at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006658">
        <property type="term" value="P:phosphatidylserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF8">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018269">
        <property type="entry name" value="PC_trans_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR018269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ46421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="453" mass="51458" checksum="CCB1DD2160E9EB4B" modified="2017-01-18" version="1">MGIDNKERLEKGSAESPVARSGSADAEKDRSSDLKPETSRQSECELKLDTGAVNEREKKKQTFILRTIWTLIMTVLLFATLTAGQFWTIVVVLLIQIAVFRECINITTHRAKLEDLKYTQPLNWWFLVTTIFYLDGRNLIKYFLTNFVVSDYTSVIAIKLVMHHKFLSYCMYVTGFVGFVASLRKGSLKFQFAQLCVTHMTLLLVILQGHCIVSNILHGMFWFIVPAGLVVVNDIFAYLCGITFGKTQLISISPKKTVEGFVGAWICTTFSSFLFTYIFSNVNYFICPVFDNIYTTFFSVVECDPNPVFIPQIYTLPSFLVEILHREYIQVKPVYFHTMIVSMFASLIAPFGGFFASGLKRAFKIKDFGHTIPGHGGITDRMDCQFLMGSFSYLYYETFISTHNVNVGNMLQSIIVNLEPQDIIILIQSLVTYLYKIGTIDEATFQKITSSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJY9</accession>
    <name>A0A1E3NJY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">I/LWEQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16315</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLA2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017544.1">
        <property type="nucleotide sequence ID" value="XM_019161163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJY9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJY9"/>
    <dbReference type="GeneID" id="30177850"/>
    <dbReference type="OrthoDB" id="10262320at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17007">
        <property type="entry name" value="ANTH_N_Sla2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF15">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="6">
        <location>
            <begin position="3"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="7">
        <location>
            <begin position="786"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="306"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="460"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="941"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1026" mass="114943" checksum="32194F7230FE7EA9" modified="2017-01-18" version="1">MFGSMRRTEDIQTSIKKACTPEDTAPKRKHVRACIVYTWERKSSKEFWESMKVLPLKENDVALFKALTLIHKVLQEGHPSCLIGGFKNMDWIASLGHTQSPDRRYQILIPEYVDYLLQKLRFHHNHRGFNGTFEYEEYTSLRSVSDPNEGFDAVYDLQLLQEALDHLGRDTLAPAKKNISEAITSALVPIVSETYGIYKFLISMLRGLYRSSDSVEALEPLRAKFDEQHDRLYDFYAQCTAVRYLSTLITIPKLPFDAPTLADEQKSVSPLIEQGIVQTPADYANVQKAVSSQPTGAVANAHMAGQQAYEAQQQQLEQQRQQQLQQQQQELEQQRLYWEEQQKRNSEAQQLAQQQLLLDQAQRQAQGRVAELERDILALKGQYDSDQLMLRSYDEKLQVLETELRTTSETAGEQLAAKEQQLTFLQEQIDYWKNKYESLAKLYSQLRAEHLDLLAKSKKIQQKAASAQEAVERRERMEREMKAKNVELADLIKERDRAKLELERVKTGSKGQFESLQLAKDELEQKLATLERAQSANLTAVFNQHKNEVEQLQQKLARSSLADGSSGKELEVLREKLQEKDMELEMMQQTMDETIKGLVAQQKEAGGEGGHGGLRKVSELIDAVLKSGIDRIQDGVFELDSAMQAGNVNSSPEYLSSVIEKTSGLATEFASSFNSFLVDGAEGDAGAVIGGVTNFTTSIGDVLLGTKGLARLAKVDELQEDLIDTARDVAEISEVYLESLFERNLGGLGLEEKTEAVINGNIDVQEMLQTLTSLVESLRTPSSRVDLEKVSGELSEVVDKEMAHASRVIDEASSHLISLLEHPSMSSVDVEVSKSILAAASAIITAIKYLIEASIEAQQEIVNNGRGSSSRTSFYKKNNKWTEGLISAAKSIASTTNTLIGIADGVLQNKHSDEELIVASREVAASTAQLVSAARVKSQFMSKTQDKLEEASKKVNLACKQLVIQVNQLISSKNELDEVDYSKLSIHETKTAEMEQQVEILKLENALNSARKRLGEIRKFSYRDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJZ8</accession>
    <name>A0A1E3NJZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit E N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_72541</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017585.1">
        <property type="nucleotide sequence ID" value="XM_019164230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJZ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJZ8"/>
    <dbReference type="GeneID" id="30180917"/>
    <dbReference type="OrthoDB" id="417252at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070196">
        <property type="term" value="P:eukaryotic translation initiation factor 3 complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019010">
        <property type="entry name" value="eIF3e_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09440">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01186">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit E N-terminal" evidence="4">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="442" mass="51897" checksum="B4D4C0A70D7CC830" modified="2017-01-18" version="1">MSQEILTQKQVETARKYDLSARFFDFLDRHLIYPLLDNLVTIYDEKEIDQMEFDILKDTYMIKLLRDLYLNLHPEAEDLPEEILKKENEVIAKLVPLNESTKKTLDILCSKEVQENLKQDKYSNRDLIKSHGIDDKKILELYEFGKLQYNRGDYVMASDLLNNFKLLSTNQELILNATCGKFVSDIMSGDWAEAKNEMSKLREVTDNRNYQGSSLEQLKLRNWLIHNSLFIFFNEKELKEQLANKETCSQLLQMNEIFISSSYLSTIEASCPWILRYIISSVLYTKDYRRLRDLIRAVDIESYEFEDPFTQLIDALFIKFNFSQLGDIFENIKILTETDFFINHLDQKQLLKNIVELILRNVLKIYKTLSVSNFKAHFINGISDELFNEIVESNNDISLDASSNTLSLKKDKNDPYFQVYERTKALSFKSTQFLNNAFGKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK60</accession>
    <name>A0A1E3NK60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TAFII55 protein conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_22447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017061.1">
        <property type="nucleotide sequence ID" value="XM_019162262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK60"/>
    <dbReference type="STRING" id="763406.A0A1E3NK60"/>
    <dbReference type="GeneID" id="30178949"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08047">
        <property type="entry name" value="TAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037817">
        <property type="entry name" value="TAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006751">
        <property type="entry name" value="TAFII55_prot_cons_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228:SF0">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID 55 KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04658">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01370">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII55 protein conserved region" evidence="7">
        <location>
            <begin position="70"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="286"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="433"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="466" mass="52622" checksum="093A5E48E8395755" modified="2017-01-18" version="1" fragment="single">PTLKIKPSKSSTSLPKIKLKTSLETTESSAISTVNSTKPKRTPTIKVKPAKLPGNGYDSEDPDKEDDPLIEEAIVLRFLPDETLDTVRAAVESAERGDKYSSADANGRRSTDLSSISVTWSDKRRAIVRINDIMYAAKLINLPTISEVHKTIDRKNIFKTVDVSQMLLVVKRIYSEREILDLKIDKEYGETYPDGLTPPMEFVKQRFDKKYEANVIQNVEDEVARLLKLDSEADSSVFEFIDAEEGEIVPMSVIEGKIKNKRAKKEKKRLKKEHIDNELDRLMKEDLDDDDDDDDDDDEEDDDDDDEEEEEEGEVEADGADGDSESEEEDDDDDDDDDDDDDDDDIPVGGTVRTGDNEVDEFTQHNALLAEEIIELESTIDQKQKDLKKAINPIMKNRILDVISRLQQELTIKKSQAAAEEEKSKMYSAEGAGAEEGDRVEDVEDEEDEEDEEEADEEDEVEAGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK66</accession>
    <name>A0A1E3NK66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16409</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017658.1">
        <property type="nucleotide sequence ID" value="XM_019161195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK66"/>
    <dbReference type="STRING" id="763406.A0A1E3NK66"/>
    <dbReference type="GeneID" id="30177882"/>
    <dbReference type="OrthoDB" id="1875589at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL14" evidence="4">
        <location>
            <begin position="47"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="132" mass="14750" checksum="3387CCAFD971D725" modified="2017-01-18" version="1">MSTETKVVSTNWRLVQVGRVVLVQNKLAAVVEIIDQKRVLIDGPSVQRQSISLGKVVLTPLVLDTLPRGAKTGIVTKKWAASEIDAKWAKTSWAKKIAQRETRSQLSDFERFQVLVLKKQRNFAVKKALAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKA6</accession>
    <name>A0A1E3NKA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017663.1">
        <property type="nucleotide sequence ID" value="XM_019161196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKA6"/>
    <dbReference type="GeneID" id="30177883"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="375" mass="41422" checksum="16254A8B6F725167" modified="2017-01-18" version="1">MFSKVENIFLCAVFFMLDKHDMTVEGMVSSFGSAEKTKYDNFVNIFRMIESEDYRQKLTAEDEAAVAPEPEEGVKQEKVPDQTAQKSRLGSQSRQEEERGSRKRVRFAGDEGAKQAEDDQAATDGAVVACETQDAAEPHTKRPRPESVHYTEQVAGLVSELRTSINDYTLLLLELDQQHTRTANTGLTREQITAELSSALRRLRQYDDAAVRHELGRGAAPALCVQLHGDLERFRFKRYEVQSRLPPGVLTDHKAPDDSSVFADSDDSDGSADIVPATPLEQQLAQRIDAVRAVVAASLDGPDGHVPVSTDTLDRAVKAGVFISRRCEQHLTAVLARATELCVAASDATRAARERLLELEERGETGPEQEVGVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKI1</accession>
    <name>A0A1E3NKI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="13">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_16474</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Oxidizes long-chain alcohols and, in the presence of glutathione, is able to oxidize formaldehyde. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>S-(hydroxymethyl)glutathione + NAD(+) = S-formylglutathione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-(hydroxymethyl)glutathione + NADP(+) = S-formylglutathione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-nitrosoglutathione + NADH + H(+) = S-(hydroxysulfenamide)glutathione + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:78371"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="13"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017742.1">
        <property type="nucleotide sequence ID" value="XM_019161223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKI1"/>
    <dbReference type="SMR" id="A0A1E3NKI1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKI1"/>
    <dbReference type="GeneID" id="30177910"/>
    <dbReference type="OrthoDB" id="417550at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033833">
        <property type="term" value="F:hydroxymethylfurfural reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106322">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106321">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080007">
        <property type="term" value="F:S-nitrosoglutathione reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033859">
        <property type="term" value="P:furaldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000001">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02818">
        <property type="entry name" value="adh_III_F_hyde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF12">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE CLASS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0520">NAD</keyword>
    <keyword evidence="5 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="15">
        <location>
            <begin position="34"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="14">
        <location>
            <begin position="205"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ46629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="381" mass="40827" checksum="1AF58276773B8994" modified="2017-01-18" version="1">MSTVGKTIKCKAAVAWAPNTDLSIETIEVEAPKAHEVRVKVDFTGVCHTDAYTLSGQDSEGAFPVIFGHEGAGIVESVGEGVTNVKPGDSVVLLYTPECRECKFCKSGKTNLCGKIRATQGKGLMPDGTSRFKCDGKPLFHYMGCSSFAQYTVVADISVVAVDPKAPMDRTCLLGCGITTGYGAAVNTAKMDEGDTVAVFGIGCIGLSVIQGAVEKKASRIIAIDINDKKKEWASDFGATDFINPMKDLKEGETIVSKLVEMTDGGLDWTFDCTGNVQVMRNALEACHKGWGTSIIIGVAAAGKEISTRPFQLVTGRVWKGCAFGGVKGRTQLPGLVEKYVDGTLKVDEFITHRHPLEKINQAFHDMHDGDCIRAVIKMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKK0</accession>
    <name>A0A1E3NKK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017762.1">
        <property type="nucleotide sequence ID" value="XM_019164276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKK0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKK0"/>
    <dbReference type="GeneID" id="30180963"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF12">
        <property type="entry name" value="REGULATORY PROTEIN CAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="852"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="905"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1012"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1153"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1205"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="862"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="917"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1021"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1156"/>
            <end position="1169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1273" mass="141467" checksum="713312D6448954E4" modified="2017-01-18" version="1">MTTDTTTADSAPCENSCGDPHEHAGGNTDDAKLSTSTSASSSSSETSGSSAPTSDSACDTSASSSFWNIIKNPSNGRTRISQACDRCRARKIKCSGKSENSPKCTNCEKDGFPCVVSDKLTRNSFPKGYTKNLEKRLLDVELSRNQLLMELNSLKQKMSSIEPSSTMENASVTDNRSTKDLSGGAENAFSNGSASNPAGSNLAAEAQVGGFQDLAASPVPNQNREVITTTTATTVAEESPLELQERSIQALRKKIYYSKPISTTRSGSLVLDQNLKSPFETIFTSLKSNPFKGYKSNASFSSGKNAFSSAAASTTSSHSATSSNDDLDPASLKHLSNYHMNLNRYLNLILYKLILPLFNASSNNGSSDTNKNLDHLIWLFFNDYNKLIPILDFELFYNDYLTFVNTYTIKKSTYSDNGELKKRYYEFNSKDQDTMIKLILILKFTLCQPKINRNNKSSAYLPDKSSLNHLDESVKLINVKHLIMMLKNINFSLSPNLDKLEISLLLFYYLIKFENFNLPNYTDNLHSHKEFLMDVINLNKHLVYTLNIDKPNALLMSSKFPEKQLIEIQRLKLYWNFKILIKLSEIYFNIPLLDEEIVPNSTQDDEDEVMSDISSAFFDDLHPSTVKKSSPASLETIVLVSNDVKITICLVKLLCLIPWNIMQFVNDENTEVLNKIDVDLSNWKDSVESLNNDENSVVFNKLNSYYLYFKVLVNINGTIDSSYFIDFVNLVYELTFKNSKSNGNKGDEISIEASESLCLHSFNFHLVTLISITSLQNVKDKQLCQKIYKLIQLYQILINYKDVDPLITTFVTYIKENILFAFSDANTIQDETLRLFSSVDMEKANSTSLFDKKVSHKSMRTSSSLSNQSQSQSQNSGSNQNVLNQGLMNFDLNAFNLHGDSFNLNDEASNTKRRKSNASTTSSFMSSNLSLNSRESSVVSSSRRLSVSSNDSMPSLFPSATSPFHNSNNTNTSLFQNNNNGNNINFNENSITSSAKKRIMDYAIQPMRTTMNRSRSLSAESRIRSSKISDRRLSGSNHSAGFTTSRENSIDVNNASIADDMVDDDIVSRILNSDVDSEVDSDVSMILTGKPMRSSNFKRNTSILPTIPSEGTLMKIMGAMETTAFDAISNSTSESKEADFDVNSLFSVTSAFNSGTKTSNDSSNCTNTNVKDDNALSPDSNSMKSKMCDFSKAFKKSTSGSISSAYHNTGASKPSHPLNNELKIADDDPLNPGNITNSNLQNFNSITNDKSIIDDDDLLRLKEHVMSMKTSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKK7</accession>
    <name>A0A1E3NKK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_11634</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017764.1">
        <property type="nucleotide sequence ID" value="XM_019159818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKK7"/>
    <dbReference type="GeneID" id="30176505"/>
    <dbReference type="OrthoDB" id="4484309at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="67926" checksum="03FCD3D09108138C" modified="2017-01-18" version="1">MSEINSPKKPKHRNNLFPEAIKPYSDYNSHKPKPNSHSNLNGKANGNANANGKTGQSSDSKLSSSSLSSSVNTRNSQKIKDFIEQLYQLKPDQTILGDKANQLEFTNADPPNGLITKTYLKSLASIPQAWVEEVKSKHSLAVSFINTEVLADSQKDEEPESASSKHGAVIIGGAENDYTWLALISTRLFRKAGGVLPIEVMLPTKEDYLKDREICDLYLPQLNAKCVIIKDLLDASTATNAELDDDLNKYIWNNFTLIKRELAILLSRFENVLFLSPENVMLKPMEYAIFDKQFYKDFGLFVWGDYGARTTLPAFYEIADLEVKNAKTSVYGLPLTEELISKSAKLTSEELSDGVNFHDLENSLPYKQSDASELIIDKKQHLGTLLLSLYYNLNGPDAYYVLLTGRFDGAHGAKETILAAAHVLSKQYYLINSEVESNGYWYDDEFKGVSMMQFDPIVDYYSYEAFIDKFSRKEGYEVSYNKYQRWLKESSERRSPLFLNINNPPLKPIELIQQGTVVKENGDRVRLIADTSYFDAEFETDIWRVMNDYICHLSLECSYVKTWFGKGNSKKRDAFCKGDIKEHLLWVSSLGSTADADLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKU0</accession>
    <name>A0A1E3NKU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 16">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_16570</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Mediates ubiquitination of proteins derived from mRNAs lacking stop codons (non-stop proteins) and other translation arrest products induced by poly-lysine sequences and tandem rare codons. Ubiquitination leads to CDC48 recruitment for extraction and degradation of the incomplete translation product. May indirectly play a role in chromatin function and transcription.</text>
    </comment>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the LTN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 16"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017852.1">
        <property type="nucleotide sequence ID" value="XM_019161262.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKU0"/>
    <dbReference type="GeneID" id="30177949"/>
    <dbReference type="OrthoDB" id="6108at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054477">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054476">
        <property type="entry name" value="Ltn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054478">
        <property type="entry name" value="LTN1_UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22958">
        <property type="entry name" value="Ltn1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22999">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23009">
        <property type="entry name" value="UBC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1500"/>
            <end position="1547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ46739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1556" mass="178297" checksum="A3348482CC030376" modified="2017-01-18" version="1">MSTAFGDTQHTGLGTNGFEVSLNYITGIPDPNLLLCSNTLKLAFKSLLKRDSSTKEKSISTMLEYVRGNPTELNDDLTIMTWVQMYPKLSIDDSKKVRSSAHQIQSQFVFTLGKAYVKYLRDTIGVWLSGVFDTDRSTARACRESIDFAFGHKKEKISNLWKIFTPQIVHYSHQVLAYETKDTLSDERFSTKDESEAKYLRVLQASILLVVHTLAENDVASLNENATSLLKSIFSQETLMEAFSSKDFSLKKSAYMAFKSLVISKHVESIIDQHSYKALSKAMIKGIKFDSKVNVILYSNVIITIMDTWVCATSYNPAFWSNIKKADAKLLSLLEIGSLNSEPIYYDIVYRLLKTLPTEVLDLKDPSMVEPYFDAVMRSVEKEKTVQFLEKGWKVMISIIQTLVTGSKVSDKILDEFTFSLVKLMDSPRVLSPILVGLMHEIHTFANDNKDVLLDVNSSVMDALPDKHIVFGDFANYTVKNTRCFVESFINLFVSNKSDLEEVLMANSIEAVEEISTPSVSPTLSFTVINILIKKNAVQYSDPINSFVETIPKQISETFVDCPLETLALYSHSSFADEHFVNSLINQTFVSLQELNLVNKLLKIIPNLGHFNLHEATKLNNYLVENSKSLSREGNTSGNIGNEDHGSLYQFLTLEILLNLYQHESFSSFILNCSKHYDNNLFLNFSKKNTKFIEQLLSIIMKKNNSDDTDYLAGSVLLDKLEENLVSDDTFAQLYQNSLLTAIGKSDLEFSSLESRLAKLPEAFIKNIIQKSNVDELKEISQFKLSNLLCIGNAFDLSSYLFTENYKNSEAIDFEHAKTIINKAVFYGELFKIHTLSDSITETNIINLSLVSEFSSDILFLNTTLSSIYEDKIVEFQTLARTMLLDLFGSSSYISIVQNLISDTNDENHLTYLLSLLHENCQVRSYYAHRVLKKILVEKADQLPNETFEKIDFKPFINHANLLFTILDSSKQHLTSKNLEYTRTNTIANLISIRKPSDICFNGMQELLILNAFINVDLNNHIPDNFTMISPQRCLNLLSTISTWLDSEVTYEEGFKPVRIVMMQFIQRYIDGIYYVCDSNYPSDFITKVFDLGAKLLSETINLVNSEDQVPLDLLSWSLKLYIILNKYRRDIENWDDECSDTEAEIIELFFKVSKISHINQPIEIVCSQFSHILTELVESKTLAKNYQKFYSLMESKNIQILRLGCTLLHQLIPEVQDNLVVEFTLSKKKADEKGNSGIHLPLVLLHISSSPLTDYIEFEEPWKVYQYLWAWYLIMDHFKNITQQMRQDYISDLGEERICLFLNFIFGDLDINRFKVHEDDKTYVKNYDLKDNSSLNYEEETKKLLVNLAYEIMNNIGGTFAQNWFNSIKDKQFQQSVEKFIIRFISPELINDILSTLSDKNVLEDPEFKININRKTNEIKCLYNIDEQKMEISILLPSNFPLSQITVNGISRVGVDEKKWKSWIMSAQYVINFQNGTILDSIKHFKDNVTANFENYEDCAICYSILNAVDHSTPNKVCPTCKHNFHSACLYRWFKSSGSSTCPLCRSKFQFKKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW9</accession>
    <name>A0A1E3NKW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_59009</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017897.1">
        <property type="nucleotide sequence ID" value="XM_019163338.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKW9"/>
    <dbReference type="GeneID" id="30180025"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF97">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="405"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="589"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1209"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1261"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1352"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1378"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1464"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1508"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1543"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1612"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1649"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1703"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="12">
        <location>
            <begin position="215"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1768" mass="204362" checksum="66E777D0EC665993" modified="2017-01-18" version="1">MDHLSIYTTFSDNARAELVSALSEKTAQPFSAFKTPYLNTSRWNHIDKLGDVFGAQFFATSKEHGYEDFAERGISDTYDNNYEPYPAWSAETAPITRDRIQSIFIHISKIFGFQYDNTKNMYDYLMKMLDSRASRMGPAKALRSIHADYISGYNSNYRKWYFGSQMDVEDNMLSENPSLYESDVKTRKKLFASQNYSLKDSETNWANTTNSFLPEDCIIQLAIYLLIWGEANNIRFMPECLCFIFKSCVDCFYSLDFTNDIQPLTESFLDHAITPLYEFYRNQLYSKIDDHWVIKERDHTNIIGYDDINQCFWYRKSLEKIELYDKQKLMDFQPFERYLYLSQINWQKSVSKTYHESRSWFHVLINFNRVWNIHLGVFWYYTCFNSPTLYTERYLITADNQPTALATLTCLSIAGSIVSFVNLVSLILEFIFVPRHFPGALPLLSRFCFVTACFITVTLPTIYFFWVTGTSDSSPLNYAIAVSQFIFSLLVVSYFSIIPLSRLTGNNHSEDKRKYLPSYYFTNFIYTLKGKKALASLGLWGGVFVSKFTESYFFLTLSLRDPVRELSIIKIDKCLGEEWFGPILCEQEAKIILALMFLTDLVLFFLDTYLWYIIWNTLLSVARSFYCGVSIWTPWRNMFFRLPNRIISKILNPAAVGKRTEVERKNMIFKVWNSIVISMYRDHLISVDHLDSLIYQCTKGENGEKIVAEPSFFIQHEDGDNISNRNDYLKSDSEAARRLSFFAHSLSTSISMAMPLDKMPSFSVLIPHYSEKITLSLQEVIRREDDFSNITLLEYLKHLYPQEWHNFVRDTKLLASEQYSDNEKEEKDVSDVPFYAVGFKAATPEYILRTRIWASLRSQTLFRTISGFMNYSRAIKLLYSAECEDQSVSTLVNIEEANVMAQRKFRIVASLQRLKEFSPEQEQAKEFLLRTYPELQVAYLDVERDPVTNEFIYYSALIDGNSDILSNGQRKPKYRIRLSGKPILGDGKADNQNHAIVFCRGEYVQLIDANQDNYLEECLKIRSVLNEFEEYDAPDNPYTYDPKDMSVSVDYIHPVAILGTREYIFSENIGVLGDVAAGKEQTFGTLFARTLAQVGGKLHYGHPDFLNTIFMCTRGGVSKSQRGLHLNEDIYAGMNALMRGGRIKHCEYFQCGKGRDLGFCSILNFTTKIGSGMSEQMLSREYFYLGTQLPLDRFLSFFYAHPGFHLNNVFILLSLKMFMLFCIHLGALTNDSVLCSYNKNIPFTDPRVPSGCTNLVPVIDWIQRCILSIFIVFGISFIPLCVQELMERGLWRCMYRTFRHFTSLSPMFEVFVCRIYSQSLVTDLAVGGARYIATGRGFSTARMSFSVLYARFSYESFYFAGTLSLLLLYVSLVLWKFSFLYFWATISSLFFSPFWFNPNAFNFTEFFIDYRNFLKWLTSGNVRTKRDSWISHVRATRMSVTGSKIKRSKGDTTFMGSNYQRPSFLSSFIAQIGSKVVTTLLITIAYLFTNSQNQSGGGVPSNSLLRLLVVTFGPIAINAGVLFCLFIVSILTGPLIMCCSSSFPGMIANLAHMISVFVYIFSFVFLLLCQNWDVAKSVLGACASFNLQELFFKLVTILVLNREIKDEKSNKAWWTGSWLTSGLGWHIFTQPFREYICKIIEMSYFALDFLISHFILVCQVPVLFIPYIDTIHSMLLMWLSPENMLKKPIYSSAKRRMRMRVTVTYFFLFVSNICVICSIVVVPIVFSRVFEVDFDEYMPEFLSILFQPAEITDKRKGLQNYIPPKLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL45</accession>
    <name>A0A1E3NL45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_59067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017970.1">
        <property type="nucleotide sequence ID" value="XM_019163341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL45"/>
    <dbReference type="STRING" id="763406.A0A1E3NL45"/>
    <dbReference type="GeneID" id="30180028"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF8">
        <property type="entry name" value="NUCLEAR MRNA EXPORT PROTEIN THP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="54902" checksum="E09A2491871CD253" modified="2017-01-18" version="1">MQQSYTLDTLFSDLDVTINQKGHRRTFEELFSINFVKYDASRIASLQLAIHQLSEKDISSIIDNNPTVKKISWPAFENFLLNYLICVRDLDPWSVIRSIDLMITVFECQSILFNPKTAFHDQISKQAIPYFEESMALMIPLSKFIDIESMHLHNRNYDYPRLTHISTILLKALNNIRSTPDLNDRMNVDKINLLFHISTSLCNVYYKIGSPILCSNVFSNVNILNLNRRYVSLSSLVTFRFIMGKYYAHQSNFMVSFHHLRACFESITVDNCPMTNILIILKYIVPVGLIVGKVADINRIRARLLKNGAQNDETRKLDALLNIYQPLVSNYKVGNCYGVYKTIFDNEAYWKHIGLWIPMLQRLRIPIFRNLAHQLWKMNGGNLTFGMLKTGLRVSMQGMETLGTAYQVKEDNYLEVDDLFVDNVLASIVFNGFLKMKMNADRTLYFSKNDIFPDMHTVLSGRFSLNPREAWLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL47</accession>
    <name>A0A1E3NL47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16658</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017953.1">
        <property type="nucleotide sequence ID" value="XM_019161296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL47"/>
    <dbReference type="STRING" id="763406.A0A1E3NL47"/>
    <dbReference type="GeneID" id="30177983"/>
    <dbReference type="OrthoDB" id="271595at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="69"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="208" mass="23778" checksum="FDE7ECA47D9090E1" modified="2017-01-18" version="1" fragment="single">MSGMVQPTENEIKRETEKFHSRETPEIKESEYVHDVYNEIAQHFSQTRYKPWPIVAEFLGAQRDGAFGVDVGCGNGKYLAVNKKLFILGSDYSTGLIDQAKLLHHKEECNDIIVADGMNLPHKRDTFDFAISIAVIHHFADEARRVDAIAEILRVLKPSGQALIYCWALEQEKSRRGYHEGMEQDILVPWVLAKKGKHEKKEGKKEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLD8</accession>
    <name>A0A1E3NLD8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018060.1">
        <property type="nucleotide sequence ID" value="XM_019159854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLD8"/>
    <dbReference type="GeneID" id="30176541"/>
    <dbReference type="OrthoDB" id="3997736at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="262" mass="28328" checksum="70BBE8BFD16E4F5E" modified="2017-01-18" version="1">MLRLAAAPKRLAFCTSGSRHYSAASGSTGGKPPSFFHNEANLPRKIEPVPGGTGLAQKEAVPLPPKTHLEGGSGANTAVAGAKPALAVPQTVMPPKPLFADAANTPPPPPPGPSADTSNYFRSYKDFCKGMAYLGVGMGVIFFMFDQHERLDVSERQMQLMKKKQKELVLQMQTYKSKLNKVAVENAKKSVLVQGKMQMHVALLRQQLLDLGADPVTIAAAIDRFEQDVKVDVAPNTVELWVPGESSLKSLIPDPHEYSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLI3</accession>
    <name>A0A1E3NLI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16777</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018093.1">
        <property type="nucleotide sequence ID" value="XM_019161337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLI3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLI3"/>
    <dbReference type="GeneID" id="30178024"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF77">
        <property type="entry name" value="FI19380P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133413" description="FAS1 domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="800"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="48"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="483"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="862"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="897"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="914"/>
            <end position="925"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="955" mass="106864" checksum="34F47D781B266C23" modified="2017-01-18" version="1">MAVLPWLLPLALLPWPKTGQAFGFGRALDSLDEANTYIHTHEGDNVPTSSIIDVLSANESYSHLLLTLQKSDLVDYVNELANVTFLAPVNAAFERHEIPIGSTMSKDELNRFIIDDVIFREEIVGIKIVSTLNHHGSPYVELFQVPLLLDHRFDEDGENEEVYLVENANVLFDDQYLPTIDTVVLTIDDLLTDPKETVCRYFLNSLNRNTGDERFKVFSSLLLSESTCKSLRMSNMTFLTPSDLSLHLNHIERKYLSHIRGMDDKNLFISNFLVDGIIGGNLENDTITTSNWNNEKLTFSSSYLGDEIIINDEVRATASNFLLSDGIIHYFENEIFDYNDTKSFPVFTARKYLIGLEYEDFVDEIDFRKLSELIDDNTLNQTVLVSRDYGMMGSLKNGMLYHFLEGNNEINLDTDENVLLTSKYCVDTDTNSKFCQRIKLETSPEGKKVLNSNSKIVNIVPYRVGNSSIYIIDNDISLPTKLQTAIASELTGYGKSTQFLKKFNYSKKLSKDNTFYTVFLPATELWNALDLTLDYLIENPLLLEKILDNLIFKGVLYHDFQGEQEFETYGNSKVEVSKDNLLDDKIVINNSTNLTISFESQILYSNGVVHPIADEIPLPQDMRISNQELLSAQDSYEFEKILDFLNLTFVLDSAKGYSVMLPSSKSLLQENITYLLGDIQFLEKFAKLHILPPGSLDMILDCYSASSETSPLLHTDYRNSTSLTDIPTLMNGTHLTCRELASGGMMLSITEGSGNEVRILRHGITIDNSTITSGLLLLDRPLNPTWMNHNDGKLYLHLPLFAILVGILIGVVCVLLTCGCCLMLTFGNSRKGIDGSDGEGFRDDEGRIEHVISVTETMPLLNDEEGSSMDKTKSAGGSKDGVNGGTDDNISGYVINKDTTQEQIGSSRALGRSLGRDGFSKENKGSKVGRRYSTFDARYSMNASASPIDVNNNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLI9</accession>
    <name>A0A1E3NLI9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_24675</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018049.1">
        <property type="nucleotide sequence ID" value="XM_019162362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLI9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLI9"/>
    <dbReference type="GeneID" id="30179049"/>
    <dbReference type="OrthoDB" id="5550281at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="81" mass="9587" checksum="BC7A3709EF03EBB3" modified="2017-01-18" version="1" fragment="single">KKRAAKDPNAPKKPLTSYILFYNHLRSSVAEKNTELSQVDIAKEIARQWKEMSESDKNYWKGIYEKDKVKYDQEMVKYNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLJ1</accession>
    <name>A0A1E3NLJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BIR-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_16786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018104.1">
        <property type="nucleotide sequence ID" value="XM_019161344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLJ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLJ1"/>
    <dbReference type="GeneID" id="30178031"/>
    <dbReference type="OrthoDB" id="2196114at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF5">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="512"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="642"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="984"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="432"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="534"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="584"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="701"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="736"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="750"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="776"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="810"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="824"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="902"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="988"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1129" mass="129761" checksum="6B4D3EB4A003C189" modified="2017-01-18" version="1">MPAQRRARGARAKDKSHLCNKSKVNPAPQAPQSFTSRLDTFHQVHMINDEPVSYQNTASESLVPLELRPSRLGRQFSERLVTRSEIHHRSLNLMTLQSPQWLAQVGFYYTPLSIKHQFAITCTSCNEIIRDPVPLETDISAYHLAENSMCALSAVWKFRNIINDSLRSKDMSWQQDAVFGHVSSERGTQIRRETFKWWKHRNPDVESMASSGLYYDPYVDQNEVDGNVCGDDRVICMYCGLRLEQWEEEDDPLSVHQKEMPSCWVFNFDKPVTEKEIGLDNDQRLMIESGEYNAQEDVPDYEGNPLNEDHDIEFISSDKEDAPTVISLATATTTTTNTALPVEILDQDEVEEFLSNSQGRHREENNRLVDIPSMKSSDLSQFFTELDAESESEVGASFFANNRRRREREKEQKENVIANVEPIAEESLMSTEEGKVNNEEEKVQQKEEDLSEQIRGVDKNGMENDLLANEADPQLEPEIQPAQELEIGLEIPIYENDDFAFENNDAHKSTMNEAAADSVETENNKLFNNPVDSFQHKSEKDQENKHRMSEQDKPRREKMGAVSIQDKENEISNRLFSEPTEAGESIKETRDADDSRVKHLESELKQLRMQIEALQNSQNLQLQAPTENASYKVNTNPARVHDADNFADENTSTPIQLRDKESEAHATTTEPANLLLSPELTVKRNSHGPLRDDEIVVSSVKQEENTDSEHVEVTAKKKKRKKTRKIKSNHSKSKHTLHEREEAGIRKEDVDDNKDEEEEREEDNNDERQKKRMKKNHEEEREKYKESEVHKVKQTVITVSDDPLSFKLDSRKKSKKHENKNKRQKAEISDKVEIKQEPDMGQELSIQMESGTELKMKTEQTPEIPTEADQIVGTSSLADEANNDHSELLVGNAVLTYSQPDEQLRSNTNEDKQEESPTLMRKKARSPPVTEAIPSEAQESRFDTLNALMNAGRPKSDRLNELPEENHFFIESDIMMKNQSTPYANDTKTVDMSEKFSKKPTDEADGTEHDTDCRRQLSLDEKEEKTKWVPIDSNKYREFYKDIQEATGYVKEVLDSRYELLGDDLEGLLTEFIAEIPPEQLKMTVREWIRFQEEQAVGLVLDKAELMMEQFRKDQSTAIRFLADLPEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLK1</accession>
    <name>A0A1E3NLK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Spt3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_25555</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017561.1">
        <property type="nucleotide sequence ID" value="XM_019162401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLK1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLK1"/>
    <dbReference type="GeneID" id="30179088"/>
    <dbReference type="OrthoDB" id="66982at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22926">
        <property type="entry name" value="HFD_SPT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="36926" checksum="1D19D659DF679458" modified="2017-01-18" version="1" fragment="single">DKNRYKYRVEIQQMMFVSGETNEPPERTTDLIEDIVRDQVVALLLQAQRTTQSRGQKSILPEDLIFLIRHDKAKVNRLRTYLSWKDVRKNAKDQENGDLAAAAAGGGDLLEEAANAGSVGGASSVDSKLLAAKQKKSLKLPWELEFMFTEQPLNGGLNGVVEDEKIDEEEREAVLAQIKRLKQNDERTKDMTQQEYVHWSECRQASFTFRKSKRFREWCGISQVTESRPNDDVIDILGFLTFEMVCSITEEALKVKSSMDSLSEERDARRYLFMEPSNDNKPIHVEHIQEAWRRLQEIPRSKKALYNFRGGRMLRTVRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLM1</accession>
    <name>A0A1E3NLM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ESF1 RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018133.1">
        <property type="nucleotide sequence ID" value="XM_019163841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLM1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLM1"/>
    <dbReference type="GeneID" id="30180528"/>
    <dbReference type="OrthoDB" id="431825at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25121">
        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="2">
        <location>
            <begin position="167"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="602" mass="69147" checksum="1AD6E47565FB0492" modified="2017-01-18" version="1">MAKGERGMDDPRFSKVFSDPRFRTVKKKDLKVTLDDRFNKKDLKKEFSLLKSTAKVDKYGRKISKDDGSSVIDNLDKYYTDKHEEEEEEEENDYAKADDYGNDDDASSSLSDSEDVKADPLAKARGLLEDATSASDLDSSDEEVSELEEIPSSDVEETENIPEQDPTTRIAAVNLDWDHLNSTDLFATFSSFLPQDGHIKSVTIYKSEYGKQRIADEELNGPPREIFRKNLGNKSGDDDDDDDDDDEDLDLAKASKKLVQENNGEDEGFDSSELRKYQLQRLRYYYAIIEFNNLNSAEEVYKKCDNTEYESSGNFFDLRYVPTDMEFDEADIRDVCEKLPSNYQPIEFSTDSLRNSKPKLTWDETPIERVQILSKSFKKGELDDLDLKNYLASDSDEEVQDESISKYKQLVGGAGLNSVGKAGEEDSDSDVDMEFTFTPGKSSTEDSTEKESLKEGKSTIEKLREKEKERRKARKQQVKELKREAQSSKNKGKKNRPDTEEDKEDDLKIEQIGTEGKELHHFNIKDIEKLEKLKGKKNKFKNKRQRELEKELESRLEDSNQGSLEVDDRFAEMIDSHAFVGGSKLLQQERKKRKKMNKVIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLU1</accession>
    <name>A0A1E3NLU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Telomere length regulation protein conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TEL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018204.1">
        <property type="nucleotide sequence ID" value="XM_019160656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLU1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLU1"/>
    <dbReference type="GeneID" id="30177343"/>
    <dbReference type="OrthoDB" id="10258062at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.720">
        <property type="entry name" value="Telomere length regulation protein 2, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038528">
        <property type="entry name" value="TEL2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051970">
        <property type="entry name" value="TEL2_Regulation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019337">
        <property type="entry name" value="Telomere_length_regulation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830:SF10">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10193">
        <property type="entry name" value="Telomere_reg-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere length regulation protein conserved" evidence="2">
        <location>
            <begin position="128"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="516" mass="58253" checksum="EDC567F075E6280D" modified="2017-01-18" version="1">MNSITSRLESKAATIMQLGMFVADYIYKRINGKFLFTVSTYACKREEFLKIFTQLDTQLQKVSSSRPVEAAIDELLNYSKAIKISDSTDTEVAVPMTPLMVEMDYDSDNEDSDLDDPSVERKPTVAKPVFLKDLLHYFTSDPQNDKTTYDKRGIAFSIGIEMVRIKKDTPEMKYYSTKLLDSALDLDGVGFPLKEDEIFDEDQIKVAFDSWRLSFMIAICTSEPDVIFKYLLENFIKQDWSVPTRIQVLTCIGLSCRELCGKEDKFIWGKTNLDKAAGKKLPGPGHDLFLNLDLGHGQDQTKKIVDIDQENRETQLVEAFESVGIGKGKVVRKSRKLAIDKKSRDSELAQGKTMHTSFINKKLPPLYYTLSAIWQEVNSQTYGAGFNVGTLSEYLNSHFLDILTMVYSCGVPSCIELVDMSIELIAVLSGQLQAIRTHAGDEFPRLLFKAVVNGIRLLLTDNERTLSVLRSTAMLEISMLTETLAHIVNESPPLEETEKTMTAMVLQQLQGYLYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM08</accession>
    <name>A0A1E3NM08_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">40S ribosomal protein S22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the small ribosomal subunit (SSU). Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 32 different proteins and 1 molecule of RNA (18S). The 60S subunit contains 45 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018273.1">
        <property type="nucleotide sequence ID" value="XM_019160687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM08"/>
    <dbReference type="STRING" id="763406.A0A1E3NM08"/>
    <dbReference type="GeneID" id="30177374"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14686" checksum="C5429DF10EA5D076" modified="2017-01-18" version="1">MTRSSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLSVMQKHGYIGEFEYIDDHRSGKIVIQLNGRLNKCGVIQPRYNVKIGEIEKWTDNLLPARQFGYVILTTSAGIMDHEEAKRKHVSGKILGFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM56</accession>
    <name>A0A1E3NM56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018304.1">
        <property type="nucleotide sequence ID" value="XM_019163884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM56"/>
    <dbReference type="STRING" id="763406.A0A1E3NM56"/>
    <dbReference type="GeneID" id="30180571"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16992">
        <property type="entry name" value="ENTH_Ent3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="25"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="440" mass="47868" checksum="DE22F7E026A84A76" modified="2017-01-18" version="1">MDQLGKSMNNLSLWEVKSYVRKAQNAVLNLSDVEAKVREATNNEPWGASTTLMSEIARGTFNYRDREEICNMIFRRFTEKSAHEWRQIYKSLQLMEYLVKNGSERFVDDARANVSLVSMLKSFHYIDSKGVDQGINIRNRAKELTNLLNDEVKIRQERKKAKDNSKKFDAISSTSAGSRNLARRAAGPGRRVSSGAADYDDDGYENRIFGDGGVFGERFEEPRGTSIAPGAFEEYDEGQHHSSSNTNSNNNTSRNGGHSRMSKASVEASKVNARGNADLLGFGDDFGTTNGANNGGFHDHAGAEYENENDDDDFDDFDDFQSATPAASAAAPASTTSNSNNLSDLLTSSYSTNTNTNYGASSTSASAPTPAIGQPKKNDDIFGSLLMSAKQNQTSSNRPAVSKPLPTPAATSSFKYAAATPTPKPSTNNNNNGMVDLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM83</accession>
    <name>A0A1E3NM83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71344</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018365.1">
        <property type="nucleotide sequence ID" value="XM_019163897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM83"/>
    <dbReference type="STRING" id="763406.A0A1E3NM83"/>
    <dbReference type="GeneID" id="30180584"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="895" mass="103076" checksum="F45B49D0B1362906" modified="2017-01-18" version="1">MDGAQLSKKVQKRNRIPLSCSNCRRKKIKCDRRKPTCRVCEAHKITNCQYFEETLSLGNGDAGMFVKYITENKNSSDESGLSTFDINIKTNSNTSPLQKKETDVSAELKMLNDKIKRLESLIPQSTGTNNISESKADALPSVFPSPNLYSQVSLDDEKNDNLEMIRQLCEKLRISGKVRIGIYDGVDAKLFANAKIGNAEPFSWATIVVKDPFSTPLWQAVARYQQTIKNTFRTSPCPVKSNLLSNHGIDEKKDHCFLGRKILDLLPNKRAIWLFIERFFRYVYPFLPYLDQSSFIEDVERIINGNHITDLKNEELVTKLTLSQQTDTAILGSLMVVIMFAYESLLDNTGNFQDSGDSTPDDVYLSKIPQSNLLINCTESCLNEFRLLKRCQLSVLQCALLLEEYQKVDSFQSFGESDSHIYTGLLIQMATTIGLNRDPAFGGESNNSKNDLLRRKIWYGLIAGDNHQYMQTGIPPSIYPDHYDTKLPWFDEKVSNIADLELERVTIDMMRKKFLAGQEMRKLADLVTNMRENPTIEDLLVSTSGLMFHLRHRLGSFDTILMMDHQNLHYKKVEKTLYMIMYLHALSLLQPVFLHIFYYCQQIRHFDATTFFYNKVLSIWMFVIGNLHNLVYSSSKYFGTGFDLFLVPIVELVMHKGVIIFTSSYIKALIAGKKLQKSNVKNEELIKEIEYYKSEIIMKLFIDVHLPILKHLCKRYFYCWKLLKAHSFIFKSIKEDSIDYSEKFHLFNFMEFFTANNFRTLIQISNWKYYKAEIEMPSWLVGWLQNYRRDIYYIPENEPMSVGKSISSGSDISLSNDLLDGLANDLSYKQSSLSNEIEHNQFTTIDTSSKNNGNVCGSTIFNPTLENVNELIPENNMGDFFSSLGLSFDELLWLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM89</accession>
    <name>A0A1E3NM89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018368.1">
        <property type="nucleotide sequence ID" value="XM_019163898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM89"/>
    <dbReference type="STRING" id="763406.A0A1E3NM89"/>
    <dbReference type="GeneID" id="30180585"/>
    <dbReference type="OrthoDB" id="2441642at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013927">
        <property type="entry name" value="TF_Opi1_Ccg-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08618">
        <property type="entry name" value="Opi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="638" mass="71194" checksum="D8F50698F0EA6DF6" modified="2017-01-18" version="1">MVKNLADSDAPVNPFSEKIAASSAEYIVADKDKSHMDQYSPRRLPLKIPEMLRSNSIDNNGSSSTSTLKDKLSETVKPLKNAKEMAKIKQKKSYSSITGSDDNNSGVAEDDLLLAASALEKLKSDKPLPATPATIPSRSTSPSEQTHNFSAPTAPKPTIFDKMISHPLISNSINYVLEKTINSSNNNIFQGSNSNPVLPSVDAVTPIIELPHLSHEVNKIDTNIGGMAKKRRRELESVDPLDASHRNKKTKATMLSTVPTLPNRDVRFRRKNGRQNLRQQIAMSSAVSAQKSLRDLKHLSSLNLNIESRKRLTMLIHFLKLGNSQLSERIDNLVQSVDEKRQKLSAKTGSTGEARSNSEPDINIQQIKSDIVTTVKKIVNVVSKVSAQSLSEPARSNVREALLKLPTNWATMLNNESVFSVGVDDEYDFNSEEDDDDEDRDDDDDEDDGDEDDFKEAHETRVLSSSPTGISEENSNGGDFENIPTMTVNNQTIFQRKRKPSISRRLLTSLLRYRKSKGGGLEQNTLKLKKWFREKIKDQIMHDSSGKVLILAQESLDMVNKIIKFCNESLDKAEHWNNDRQHQHQVELLQRLQQINGFNLSLIPNKGKEEIIETMVVKENATTNTKSSSAEDDAKKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMD4</accession>
    <name>A0A1E3NMD4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_110194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018398.1">
        <property type="nucleotide sequence ID" value="XM_019159739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMD4"/>
    <dbReference type="GeneID" id="30176426"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="86" mass="9241" checksum="AAD74450632B8C7C" modified="2017-01-18" version="1">MNSSCKKTVVRNINSFAPFGPGDLATLHSTYMHITDGQLLHQIPDVFKTSPCASSSSSFFFVRRGLRVGSRGAQKLSHGVWGGGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMG6</accession>
    <name>A0A1E3NMG6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018404.1">
        <property type="nucleotide sequence ID" value="XM_019163907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMG6"/>
    <dbReference type="GeneID" id="30180594"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="57" mass="5369" checksum="727FC3B870100D15" modified="2017-01-18" version="1" fragment="single">DGPADGTNDGTDDGAEDDLFDGADDGTDDGTGDGADDGAEDGTDDGTDDGAADGADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMK7</accession>
    <name>A0A1E3NMK7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018478.1">
        <property type="nucleotide sequence ID" value="XM_019160770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMK7"/>
    <dbReference type="GeneID" id="30177457"/>
    <dbReference type="OrthoDB" id="3998144at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="366" mass="38581" checksum="F316D1005E7C1379" modified="2017-01-18" version="1">MAAKKTKLVTLKLSPEKLSGFPDLAAHVHKPKPRSKPTKAPQAKLKLNLSSPEVVSREQSLKPAGSLSDVQDGSNSPVATVGNLSNLNNIGNNNANSGTVGTGSASATSNINAGSKLSSNVRVGVSGLVMNTSIVRELDKSGRRTSKWAKFDTIPGTEGNNNSNENTGELANKSITNGAGVSETQKDATTIETANITIANAASITAKANSISVSSHKKIPEVGELDKNNGVISMRDLRGFKEHELIGKENQLIEEARKRGRKVVRSFNGYLMLWPSWHKTEEGNLEKKLNGTSDSKQGNLKKKDTKTTSLKNSPTKVSNGLESATPSVTNLEAIAEIATDANAADSGPATDAKSSILTSSAVKMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMR0</accession>
    <name>A0A1E3NMR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Srp40 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018504.1">
        <property type="nucleotide sequence ID" value="XM_019160781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMR0"/>
    <dbReference type="STRING" id="763406.A0A1E3NMR0"/>
    <dbReference type="GeneID" id="30177468"/>
    <dbReference type="OrthoDB" id="5599646at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="328"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="400" mass="41880" checksum="E7F60D3973B4B3F0" modified="2017-01-18" version="1">MAATKEYVLGKIFKYLCAEGLQSSADTLLKNAADSGITIDTKETGAERVGLDRFFDNKALISEDEKLASAAEKKASEDSSDSSDDDSSSSSSSSSSSSSSESDDSSDSDSSDSSDSSDSSSSDSDGDSDSDSDSDSDSDSDSDSDSDSSSTSSPEFNVEAKEEKSDKEEKTTELAKSSDTSSDSSSSSSDSDSSSSSSNSNSSDSDSDSDSESSSSSDSSSDSSSNSDSDSDSDSSSDSDSSSDSDSSSDSDSSSDSDSDSDSDSDSDSDSDSKNVKKRKLEQETEEKTNKKVKASDTESETLSEEVSPDPEHIPAAEEELRPGQKKHFSRVDRSKVSFEANELTDNTYQGAAGTWGEIANQKLGAVRGKDFTKGKNKMKRGSYRGGSITMAVGSYKFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ1</accession>
    <name>A0A1E3NMZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_57945</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the ATP-dependent dehydration of threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HesA/MoeB/ThiF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018593.1">
        <property type="nucleotide sequence ID" value="XM_019163266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMZ1"/>
    <dbReference type="GeneID" id="30179953"/>
    <dbReference type="OrthoDB" id="10265862at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061503">
        <property type="term" value="F:tRNA threonylcarbamoyladenosine dehydratase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061504">
        <property type="term" value="P:cyclic threonylcarbamoyladenosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00755">
        <property type="entry name" value="YgdL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000125">
        <property type="entry name" value="tRNA threonylcarbamoyladenosine dehydratase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267:SF2">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="14">
        <location>
            <begin position="34"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="414" mass="46214" checksum="3774B11180A8C4EE" modified="2017-01-18" version="1">MDYIRTLLPNGKGKDVPETITPYSEELILEQLTRNYSFLSSPGMAKIRALRVIVVGAGGVGSNAIVSLVRSGVSHIRIVDFDQVSLSSLNRHACATLKDVGRSKVECLVDFIGQIAPWSDAEGINELFSVEKAERLLRTMEDGSKPDYVVDCIDNIDTKIDLLAFCYKNDIPVISSMGAACKSDATRINVGDVSVSDEDPLARAVRRRLKKLGISNGITTVFSAEKPDPRKASLLPLADEEFERGKVDELSALQNFRVRILPVLGTMPGMFGLAIATRIVTEAGGYPVEPIEGKNRYKVYDSLLQSLAGQQTRIDIKDQRTPWVALSDVQYIIEEVWRGKSPVSGLSTRLTLSRWYPDQELGMQNAVVLTKEEQREHEKRVLLGNEKIEDVYSPEVVRRVLDRFAEEKYYSQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN13</accession>
    <name>A0A1E3NN13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S acidic ribosomal protein P2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_71155</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018141.1">
        <property type="nucleotide sequence ID" value="XM_019163846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN13"/>
    <dbReference type="STRING" id="763406.A0A1E3NN13"/>
    <dbReference type="GeneID" id="30180533"/>
    <dbReference type="OrthoDB" id="1227494at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="10771" checksum="58AE402AB8A0FE3C" modified="2017-01-18" version="1">MKYLAAYLLLQQGGNASPSAADIKTLLESVGSEVDQSNIDLLLSSLEGKSVNDLIAEGATKLASISTGGAAAASSGAAAGGAATEAAAEEEEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN77</accession>
    <name>A0A1E3NN77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15496</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018679.1">
        <property type="nucleotide sequence ID" value="XM_019160848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN77"/>
    <dbReference type="STRING" id="763406.A0A1E3NN77"/>
    <dbReference type="GeneID" id="30177535"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001400">
        <property type="term" value="C:mating projection base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072687">
        <property type="term" value="C:meiotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032160">
        <property type="term" value="C:septin filament array"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="7">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="336"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="395" mass="44912" checksum="B1F9CB7FDC235972" modified="2017-01-18" version="1">MVIGQSGSGRSTFINTLCDQPIVDSSSTVSLYSPEELSYPDRELQLRKSNVELEDNEGVKISLNIIDTPGFGDSIDNNVSFKIIKDYIKYQFDEILVEESRLRRNPRFKDGRIHVCLYFVAPTGHGLKEIDVEIIRQLGDYVNILPCISKADSLTVEELKLNKRLVAEDIEHYKLPVFDFNNEYFSYDDNLDDDTAELNKYLQRAIPFAVMGSNATTKDPVTGEIKRIRKYPWGTVDIFDNDISDVATLKNTLLITHLNEFKDFTHEVLYENYRTKTLGDASGPGLDVNDSIHTANTSTSNLGTPSLSAGAAGRNISQSTTRETGLGDYANDTLDHNQTDTDIQLKEEQIRKEEERLRAFEERVQKDLLLKREEIEARERELAELERRLAEQNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNB9</accession>
    <name>A0A1E3NNB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exportin-5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_30943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018712.1">
        <property type="nucleotide sequence ID" value="XM_019162663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNB9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNB9"/>
    <dbReference type="GeneID" id="30179350"/>
    <dbReference type="OrthoDB" id="2215036at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="1">
        <location>
            <begin position="369"/>
            <end position="1195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1240" mass="144033" checksum="28690CD3B3F64330" modified="2017-01-18" version="1">MSSEGINQIIASLDIIYNSSSNNNQRHEAQAFLESIKKLPDCPFWGYQLALIDNNYNNIVRHYALNLLLNSITDHYNEWDTPKKLAVRNWIVELATKVSINDPHYIKEKLAFLWVEIAKRCWGECLLKQNPSLNDWKTSKNNSNNNENVMSNIQFTDEEKLDSWISMDSNLLELWNYSPITRELSLIVMRTLFEDIYLLDDPIVSKRKAVLVSLCPEIVISERILSAKYEPNDTVRLFVSSPEGWLIKWAQLLDECIKCLLYDSIDNGKTCIGTTIKLLEIFKISLFWIIPLALREVDIITKLFQLLTVDNLKIKLLTIECFDCLYNKPYSNPEDNEWLVNLAFNDTAYEIFFKNFKCIELDSEDIDDEKYTLLKKIVEFLVILSDYITSKESIKTKDRDLTNFYKLILETTKHESLSISAISLQFWCSMLRADELSDQLDFESIMPELLEVCADRLINYNDCDPDSVALNFLSFDFDNQSDQHSFLNEYKKLIDDIVRIIVCKKPKDALIWLAERLNKFYSSPMGIESLQNANLIYKGKGSDSYIYSYSQFSIIEACVRGITRWQIWYRQDDAPEIKAYLTEQADNLCTLLMMLEIKNPVLVRKQIQTLVQFTPLLKDLNNTMFKVLEKVIESCTFPYPDNASDEELESIRDLRASSGTELNRLAYLMPESLKNIFSELEAAIDNILAQKELISHEVVAFKSFLLVVSQRSSLENKTETFSRIVDPELMAWSDPVTMKGLSDLHWFMERLGIVKIAEYFRSRGISAATNLLGAEMDDRGRQLKKELKDQWSSVFPIRASRILIQYSIEKLDHESDTYKNLLKLWQPRVKPILPYILQLIYQIQAYHNPANWVGLPEEVQTFVKYSTMERFWQQGISIQSRESFVDENVKAMHTLRDFADSVGHIVRYTREYAYLMIGTITELEETLYFESKNAVLLWKALTDESVGITLHSWRHMINLVVRNVIKNCPLDYFDFFMPVFLPQVFTKLDELLVERWSIVYQKGLRLDGNESDEQLSEEMLEEHMLRQLTQVVDRMLIDLAGQKARTSLDQRQRESREFILYNFEVLEPFLKLICDIISFRDTRCSFNAILILRHLSQELIGLNNEKVDKIINDSVIPTLINLLCDKFYADAHSEAAYTLSILYIGERFKSNYPLQLLKNALGLSEETMANFESVFGNCQRSRERKNCLLSLFNNARNEGNDTYLEQRNKMIAAASRGRKKNTVDGLDAGGLGSLFNTENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNJ1</accession>
    <name>A0A1E3NNJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Activator of Hsp90 ATPase AHSA1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018779.1">
        <property type="nucleotide sequence ID" value="XM_019163988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNJ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NNJ1"/>
    <dbReference type="GeneID" id="30180675"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08892">
        <property type="entry name" value="SRPBCC_Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="ASHA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF22">
        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="329" mass="37316" checksum="BAD421F7889FCFBB" modified="2017-01-18" version="1">MVVQNPNNWHWVDKNCIDWARAYFVEKLGSISAEDVESKINVKITTVKSLKGDCEVCQRKGKVISLFDMALEANYESSSGHKGVITVPEIMYDTEPDEYQFNLDDRVDEPTRVLIRSKIVPQIREVLKDFGPTLINVHGSDIQVSESQVMSTFTKGNQNLTYMKPVVKEKTTFNKGESETKEKKSSPLPEYAQKYNISSLHLSSEFNTSAEQLYITLLDPQRVSLWTRSPPGGIEPKVGAEFKLFGGGVEGKILRLVKNKEIEMLWRLSSWKEGHYVKILFKLNQGQNETSMDVDMNGVPIGEEELVEGNFKDKYFRAIKLTFGFGAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP07</accession>
    <name>A0A1E3NP07_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018376.1">
        <property type="nucleotide sequence ID" value="XM_019160733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP07"/>
    <dbReference type="GeneID" id="30177420"/>
    <dbReference type="OrthoDB" id="10429812at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="414" mass="47289" checksum="5D96BE0F8B3FD123" modified="2017-01-18" version="1">MANEKDTEKSKQDADDWRNIECWVEDDFDANELLRGLDSAGRGATQDNEEDWADWSGDEGLGEGKARTAAKTHDNSWVTKGKVTDDPMEKSWRKASKLEKVPRGRRARGEESRIGREKPRRLSEPHHNVSSIPHEPSHERRRRGGRRRGKEPDLFPSVKKEKQMDEMSSAFARLMPVAGWQPAAGEEARGEEEAESADEGVDLAQDIKIFPRLKTLPHVHEERLRLIQPKQVQVKEEEPRSRRHRSRHRKSKSAQKEKSPEPNHTEVSHPVRLRYSEEPKQSSKTAPATAKEKKRNEAGAKEANNKKKPILESKWAALNNDKADDTTVRMREQYRQKKIDSQLDWDNGVKGTADDHASAKEAVSAKSAPSNSLQKQHQHQQQHQHFAYENSEPPARNEHTPKSKAKSKLTSMWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPF3</accession>
    <name>A0A1E3NPF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_23016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019082.1">
        <property type="nucleotide sequence ID" value="XM_019162289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPF3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPF3"/>
    <dbReference type="GeneID" id="30178976"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="96"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="337" mass="38664" checksum="585B88FCAE254EAF" modified="2017-01-18" version="1" fragment="single">RFDVLEIHGRYENPFKEYRPQTLYEFFVMRLIELSEFGARGGVPKETSQRVEELRWVQPSVEYMKNVRDICLEHGMVAYTWLGQSCGLFQSGGKVFLMDPLFEDSIVNKTFGPKRVVPCPLKIEQVVEEIKPDYILVSHDHPDHLGDDSTSKLSTIQGRCKWIVPNGIGEFLSKHGVETSSIIEMKWWERVCIDDEFEIVCLPAMHWSGRMLLDANKTLWCSFMILRNGKSLFYHGGDTGYASGVFNKIGELYGPVKFAALPIGQYCPEWHQKPRHISPAESVKIMKEMKIHKMVGVHWGTFVLSSEHYLEPGNLLLQEAAKNERKNNIIVPSQGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPH3</accession>
    <name>A0A1E3NPH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glycosyltransferase family 71 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_21365</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019134.1">
        <property type="nucleotide sequence ID" value="XM_019162197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPH3"/>
    <dbReference type="GeneID" id="30178884"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="62381" checksum="435AAE75A405F426" modified="2017-01-18" version="1" fragment="single">MQDFANLAVENTLSFPHPERVIFNGGKPVIWDTVFIDDAYTKLSYDYLSKLMEFDNIFLKDLNLKNKIITSSLPLEDCPFYSGSGYVLLGGSTYSWYSLLAVQSLRKVGSKRPIEIIIPNKHDVDELLCETVFPMYNAKCVTFEDVFPKPVLKKLNATGYQLKALAILASSFEKVLYLDSDVFTVENPDTLFESELFQHYGLITWPDFWRRTTSPALYTSLGLKFSWKPVRFLNDYFTPNELLYQSKELKNPKQNVNLHDLKGTLPDWSTEAGLLLVNKSTHFSTLLLALYYNLNGPAGYYPLLSQGGAGEGDKETWSLAAQVLDKPWWQVNNQPDKTYGIWLRDRNWIVDSCIIQVDPMEDFEGVLGLTWIQEYWRKEMIDRGGYVYNYDYSFGKQGYEYSKVMGAADMFYHLHSPKLDPWHYVLDDLFIDMNNQPMRNFGTVWTRLGWDFEKWIWETVREDMCDSKKDIESCFQGRDYERVCNGEKGRLDKRIEWLEKDGKSALKNSGKPPSGWKLDGTERSRIRDFVEDRWNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPK2</accession>
    <name>A0A1E3NPK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15896</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019163.1">
        <property type="nucleotide sequence ID" value="XM_019161010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPK2"/>
    <dbReference type="GeneID" id="30177697"/>
    <dbReference type="OrthoDB" id="547796at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032443">
        <property type="term" value="P:regulation of ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000003">
        <property type="entry name" value="membrane-associated progesterone receptor component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF76">
        <property type="entry name" value="CALCUTTA CUP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133443" description="Cytochrome b5 heme-binding domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="54"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="172" mass="18924" checksum="C852FECEA80D339C" modified="2017-01-18" version="1">MEGYLVAVLVVLVAAAALKNVLFSTVSHVDLETEIDPERDSSLAKEAPTVVREFTPRQLSNYNGFDLEKIYMAVKGSVYDVSKGRQFYGPSGPYSNFAGHDASRGLALNSFELEHVRRWDEPIDDLADLSELERKSLDNWEGMFKGKYPCVGTLVPDPDFAPQPPAAAAADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ3</accession>
    <name>A0A1E3NPQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Conserved oligomeric Golgi complex subunit 6</fullName>
            <shortName evidence="10">COG complex subunit 6</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Component of oligomeric Golgi complex 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15878</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Acts as component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the conserved oligomeric Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the COG6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019148.1">
        <property type="nucleotide sequence ID" value="XM_019161002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPQ3"/>
    <dbReference type="GeneID" id="30177689"/>
    <dbReference type="OrthoDB" id="272987at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010490">
        <property type="entry name" value="COG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048369">
        <property type="entry name" value="COG6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048368">
        <property type="entry name" value="COG6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506">
        <property type="entry name" value="COMPONENT OF OLIGOMERIC GOLGI COMPLEX 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20653">
        <property type="entry name" value="COG6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06419">
        <property type="entry name" value="COG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01087">
        <property type="entry name" value="COG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric complex COG6 N-terminal" evidence="12">
        <location>
            <begin position="207"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved Oligomeric Golgi complex subunit 6 C-terminal" evidence="13">
        <location>
            <begin position="346"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="865" mass="98790" checksum="DC21CACB75EAC21C" modified="2017-01-18" version="1">MDFVYDLETFGNGSSQKKVDSPDHPASGVSLTLRSLSTSDFSKKLQSLSLLDIAKGAAKSGADSFETKNGSNLSYNAQCLKRARASIDLLDSLGFDYNPLQDASTNGSYHPNSKTSIDNDDLTDIFLYNSGFDGKSLVEKLTKVLESSDGYDTSTRHSLSILERRIEYELSLENGDNSDTKKQSGTFGAVSHFENLKNLTSTGQTGNLARRNLRGLIEEDLLHQYASQLRTFQKIVRSIESVRPNLETFTKEYNDLYSSINTSVNSLQDLRQEITSLDKQKKVVDLKKNILLAFKSTFTVTQYEEHLIRFADLNDATTGVDFFNAIDKIRSIQNNCDVLLGMENEKLGMKILKQMSDLLVSVNERITSYVQNNIDYVYSGSNLNLNISKKISLSTFHKCLVYIWQNNRSAFDSIMSDMVESRSRLIANEFINQLKGYSEEVSTSNQKTAYSKASTNKNQSRLFLSSYDTIRFMSDSLAYIHSLLVNEMENSRSFLTFDFISRDENSELENMVGSVVMRIISGLNKPLKGAVESVLRQEAKLPTLVNSYDLLELYSGMFTKLLNPEQKAMNDGTMATMKELEIETQERIFFLIKLKLKTLEVEAAEEGLNTTEDGAVLPDWVVDWFAFIDDMFEGYNSNKTFDDTNEHIVGLNDKQWNELLELLINKPVELLKSIHNKLKAPEKDKLIWVLNCVDYMYNKVEINSSLSSKADYLNSLIEKDTDILINLEFTGLLKSSGLFDIYNLVNMIFKLDDEFFDVSFYEPILENKLFSVETFQTANQKMEVFLSSYINQNELNGLMSPSIYNKVFFDSAMNFIKFYRKLCLIVNQYLRDRDDNKIKVFQWDEMTIATLLGIEEYYQEHQHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ4</accession>
    <name>A0A1E3NPQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15920</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TFB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019192.1">
        <property type="nucleotide sequence ID" value="XM_019161023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPQ4"/>
    <dbReference type="GeneID" id="30177710"/>
    <dbReference type="OrthoDB" id="364513at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2610:FF:000001">
        <property type="entry name" value="General transcription factor IIH subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00625">
        <property type="entry name" value="tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="10">
        <location>
            <begin position="506"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="589" mass="67365" checksum="811F372C6F387B55" modified="2017-01-18" version="1">MSTPQPPGSSNSQGDDEAVDFFKSTINEYLDNLPESVHQQLFQSRETCLAIFRLLPALSKFYIMAILFNETSVPYSDLNRWVKSNKNSEFKGHQNPTKIYQNDSIKRLKSLHILKEIKRQMIHPITKQPTIIIFVQLNEIFRQSFRDALTGFLPDSTQDQSKITLASSKDEKRDKSTSLNMMFDDDVDNSITVEYLDRYCIAKWENILHFMVGTEIKEIPSVGALTLLRFSGLMELPSDRERREALDGQVDEDIDYGYEDDNKKSIHTLKQLLITKTGFQFLLQDISSQIWTLLIQYLIMAEKLKMNPVDVLNFIFLLGSLELGQGYAISMLPQTQLIMLDDLQDYGLIYHQKDSSVFYPTRLATSLTSEGSSFKTASAAMDQEISQPRADKSTNSGSIIIETNFKIYCYTSSPLQIAILNLFVHLRARFANLVTGVVTRESVRSALRNGITSTQIINYLETHAHNGMIELAEAEYNKKYEFESSIGNTTALEQLKLEILPPTVVDQVKLWQLEMDRVQAFKGYLYKDFNNDFEFEKLLSYGEDIGVIVWRDKAKRKFFVSQDGNPQLIDYANKIIRNSSGTPRANTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ6</accession>
    <name>A0A1E3NPQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Meiosis protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019190.1">
        <property type="nucleotide sequence ID" value="XM_019159737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPQ6"/>
    <dbReference type="GeneID" id="30176424"/>
    <dbReference type="OrthoDB" id="27934at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28527">
        <property type="entry name" value="MATING-TYPE SWITCHING PROTEIN SWI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28527:SF1">
        <property type="entry name" value="SWI5-DEPENDENT RECOMBINATION DNA REPAIR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="41564" checksum="44B3B570362629F6" modified="2017-01-18" version="1">MSGLLSDSTNSILDPEQSFTRQQDLYGSIRPSPMKSEKSAIELPKLDGKLVLPKQRKRSNSMDARSETPSNKSRDLKARQLQRPSTCGKENLRNFSTPLPLKVGSLHDTPFPTLTPDTPQYVDLVANINKDDNEKNNASLNSLQQTPPISNSKVTSPISVRCFSTPLADIRSGSAVKTAVQIKLHQLSSDKYLEEQERQLQREILKYQSEINLMKKLKKYRETNDTEKLDRLIDKWRDIAAKGSNYLYNEARLKISRMGGLDEFRKKQKKTKLRKMKFEYDESLLYRIEEYMESEEYKNLDNYEKEEVISRKREIEEMSEKIENGDFLSDNDDDEEDELTMKELYKQLGLDYDLVYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPR8</accession>
    <name>A0A1E3NPR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_10959</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019145.1">
        <property type="nucleotide sequence ID" value="XM_019159735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NPR8"/>
    <dbReference type="GeneID" id="30176422"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF8">
        <property type="entry name" value="BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="4">
        <location>
            <begin position="6"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="269" mass="29156" checksum="08C8AB7613AF5F36" modified="2017-01-18" version="1">MTEDKKTIIVTGASRGLGKCIVQNILDDYARAQVVLIARNGDQLQAFYDGLSTEDRQRVLIAPADVTDRGAVVAVLEQTLARFGQIDGVVCNAGVLEPVGHLDEADYDMAGMRRLFEVNFFSVVQLVNETLTRTGRGTVNLVFVSSGASTRGIDGWLAYGASKAALNALCKQLHDEMYPRVRSMSVAPGVVDTDMQRAIREQLRPQMSAESHRRFVELHSGGELLDGMVVGKVYARLAVDGPPATAAGQAVCGEYVRWNDPRLSVATSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPS1</accession>
    <name>A0A1E3NPS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_21572</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019168.1">
        <property type="nucleotide sequence ID" value="XM_019162210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPS1"/>
    <dbReference type="GeneID" id="30178897"/>
    <dbReference type="OrthoDB" id="7608935at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049024">
        <property type="entry name" value="AIR2-like_ZnK4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051644">
        <property type="entry name" value="TRAMP_AT-DNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21759">
        <property type="entry name" value="AIR2-like_ZnK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="180" mass="20292" checksum="375AFE36B87FBC6E" modified="2017-01-18" version="1" fragment="single">EVTADNDHLANLRGEGRYFGVTDPDANQPVCSNCHRRGHKRAHCKVVVCHACGKVDDHYETQCPNSMVCSNCGEKGHFRNNCPSKRNQTYCVECDSRNHSSERCPSIWRSYVLRPADKNAKVAYPSAKIFCYNCATKGHYGDDCRQMRVSKTPNINGSAFSGDNLPKVLVSQYRNMLNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPS5</accession>
    <name>A0A1E3NPS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-specific chaperone A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72038</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TBCA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019155.1">
        <property type="nucleotide sequence ID" value="XM_019164081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPS5"/>
    <dbReference type="GeneID" id="30180768"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004226">
        <property type="entry name" value="TBCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036126">
        <property type="entry name" value="TBCA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02970">
        <property type="entry name" value="TBCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46988">
        <property type="entry name" value="Tubulin chaperone cofactor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="100" mass="11336" checksum="CCA0D7995ED54400" modified="2017-01-18" version="1">MSPTDKEIKVAALTRLLQDRTTYIQEVGEKEKRLKDINKHDGKNKRSDSDSNAEILLQETKNLIHLVEAKIKEVATDLRGTPNGESGDAVNRLLYEADRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ83</accession>
    <name>A0A1E3NQ83_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019338.1">
        <property type="nucleotide sequence ID" value="XM_019159760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ83"/>
    <dbReference type="GeneID" id="30176447"/>
    <dbReference type="OrthoDB" id="3997772at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="682" mass="75084" checksum="ED525A34A50D326A" modified="2017-01-18" version="1">MDGTKSGGEDEYEGHDAIDMYEAHTDSEESHLNRTGFEEHKEENDESKGESADEEEVGEQPLFLVKQGAAEENEETAFKAGEEETGDDGGSGMTEEHEANDEAEDDENDDSDDNEVEEDDEERDEHEETVFMVDENGDHIEEQEGYDLDFNEYVGPSDEEDMGETEEYDYEYNNQRESSPDLVVPDVGITRESSMMSRITNESDIFANPRKPSVAFPHPSLQTLSEEMKQFSGESDSDFISMVNGQSAKPSRTATTSSADSDSSGSSGQTGISDHHNQFISNARYSHSIMSYDDDDDDDDAFSFQANDDKEYEIGYTPSHGHDGARAASDEGDETDTNITDTESVTEDDAKTPVDEVSDAALPPVPFAPPIMGQSQQRNEKNRDSLINAEELQNEVGAMRNQFVRSDMHPSVMSKVMNLVEFTGGDDLPQLPKGMLVNGAPVDSVVSDSIADKGESREAYMADLAKSTGSETLGVPSDDELELNKTKSSGSIGLNSRLNKSASTLGGRSLDNSSSLSGSTKSLVQPVTPKNIKAGEIFARIPDVDLVQLFTNNTKVEDNIKSLREIRIELSEFDTGLTDWISYNMGDSMKINMEDDKLGVHVRKAFKLSEEGSVPTNRNTFTNIGSFSDTVENMAHEFGDFGGRNVIKFKDVVKEVKVKKIGQRGKTLLRKLKKVPEFSSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQJ3</accession>
    <name>A0A1E3NQJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Scaffold protein Nfu/NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_31278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019091.1">
        <property type="nucleotide sequence ID" value="XM_019162687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQJ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQJ3"/>
    <dbReference type="GeneID" id="30179374"/>
    <dbReference type="OrthoDB" id="565552at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="246" mass="27818" checksum="ACCFAF91100AE87C" modified="2017-01-18" version="1">MFRRSLFIQTQTTPNENALKFIPSGTRILPTQQTPTVEVTSIKDAIHKSELALKLLSINDKAISSILFGYDFITNNEVKPQNWNVLKPQIFALLTEHLTMGNTVLSNDFYDRLVEKSQSGFTGSKDEFDEDLTPEEEVVALIQELLTTRIQPAIQEDGGDIQYISFDHDKGIVYLRLIGACKSCSSSEITLKSGIEDMLKYYIDEVKAVEQVQDETFKKTEQKISDKHADTPKFEKHTVDDLPPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQQ6</accession>
    <name>A0A1E3NQQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_14013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019550.1">
        <property type="nucleotide sequence ID" value="XM_019160184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQQ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NQQ6"/>
    <dbReference type="GeneID" id="30176871"/>
    <dbReference type="OrthoDB" id="10263513at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="106" mass="11783" checksum="166D07037E6E1639" modified="2017-01-18" version="1">MPPKIQQSKAAKAAAAMAGGKKSKKKWSKGKVKDKASHLIVLDQEKYDRILKEVPTYRFVSISVLVDRLKIGGSLARVALRQLEEDGVIKPVSKHSKQLIYTRAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR07</accession>
    <name>A0A1E3NR07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SPIN90/Ldb17 leucine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019625.1">
        <property type="nucleotide sequence ID" value="XM_019163569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR07"/>
    <dbReference type="STRING" id="763406.A0A1E3NR07"/>
    <dbReference type="GeneID" id="30180256"/>
    <dbReference type="OrthoDB" id="445362at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030125">
        <property type="entry name" value="SPIN90/Ldb17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018556">
        <property type="entry name" value="SPIN90/Ldb17_LRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357:SF1">
        <property type="entry name" value="NCK-INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357">
        <property type="entry name" value="SH3 ADAPTER PROTEIN SPIN90 NCK INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09431">
        <property type="entry name" value="SPIN90_LRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPIN90/Ldb17 leucine-rich" evidence="1">
        <location>
            <begin position="285"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="598" mass="69259" checksum="FF4FFAE96C8D7F92" modified="2017-01-18" version="1">MSSKDSIELQLEGDDITPEKFWELTDDLLYTKVFYDTDTKLYFTKHFKNKYIEKTITDDTSSDDHKEQKEDKFDNDGTNSENYNLEICTEDEINTLLVSFITLVAKYCNTLIAQVGEDTFFDAVAEYLMNFKYYQTHVEFCIRKMLSLLIYSVDLNLHNLNLNVNALDDTVKDEVNDAIDTNEKFIKIIGWIFYKHYVHYKKELLETLKEYSGFTTLCKVLKNYIAISEAVQDGTDMFGNSYKNYMSLMFELCKNYNFSDELKVFQQSDIISLFHNLKVQARDEDEVNFLKFRLLLVLNEQYMFQCGLKVKYEENTNLIVEAMIEKVQYFQVFNEILILNFNREMDRVDQILMLKFLYTVFSNPATNSMVYLNDLKVIVDIIIRQLFNLQIGKAEYLVNIYLRVLYCILTETDLKNNSYKRDELVEVLKYTHSSDDASDKTKILAKRCIDCRFFQSFQNSAETLPAMSKLQIQPDSDRGRYGSCPDLTTKDRIHKIGHETHILLSPAAKLLNFESHIIQQVTRSPTGHHIISPHKVAHTLHLESGKIPFLDHLNLSSSHIGGSRNADSHTNGVANNNTKGSRLAGGTIQRLIGITVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR47</accession>
    <name>A0A1E3NR47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70166</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019654.1">
        <property type="nucleotide sequence ID" value="XM_019163578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR47"/>
    <dbReference type="STRING" id="763406.A0A1E3NR47"/>
    <dbReference type="GeneID" id="30180265"/>
    <dbReference type="OrthoDB" id="423559at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF14">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="193"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="578"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="704"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="756" mass="85670" checksum="2D451EB0732EA4FB" modified="2017-01-18" version="1">MDPKDDLSDTFRSLSLGKPDLLHKPSKSLNKPHVQTVPGQFQFSNDFLQPISVKSKQWNSKKIDVQKLQTNRGSFAKYAGKHVIHPPAAPKVVPREQLPPAVLGTTNNFERIEAIADNIADEADTIRTKELIEELLKPTEYPKVAVDPKDKIIPGLHAELYDHQVLGLRFLLKREKAKPAERDYLKTEDDKNEDAFLFNVGGILADDMGLGKTVQIIALMLKNQSKKQETSKSKTTLIVCPASLVSQWCTEIETKAPDLSVLSYHGPKRPSDSKIVHKYDVVVTSYPTLSSENSKTASPLLDSEYPFRRVVLDEAHTIKNPETKSHIACCNVLAERRWCLTGTPIQNRIEELYSLFRFLGVNKYENMQIWKKRISSPLSSHQPDDPPKALKRLHSMLDEFMLRRTKQVLIDNNVLTVSKTIHRETLDFTPFERRLYDQLEKKIVHNILGQELDLEKQNAGTLDRKQNVDLSYMSIFTYLLRLRQLCCHWELLFNLSQQFEDDSLMTEIKSTMAGLKAEKSISNRELDDALIDNDLQDLLGSMKNMNLDNGNGSQMLKEVDVGKKEKSGENAQSLHAIKLQRTLNILKKDDKVKPRKTIIFSEFTSMLDILSESLTKNGIRFVRYDGKMDKTLKDLTLRSLTDDPGIHVLLCSLKCGAYGLNITSCSRVILYEPFWNPAIGAQAVDRAYRIGQLSNVDVHEFYVSETVEIRIRELQDKKRELMQAVVDKDAQSTAKLIGNGLSKAELFGLLGLSGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR76</accession>
    <name>A0A1E3NR76_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70197</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019684.1">
        <property type="nucleotide sequence ID" value="XM_019163586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR76"/>
    <dbReference type="STRING" id="763406.A0A1E3NR76"/>
    <dbReference type="GeneID" id="30180273"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="7">
        <location>
            <begin position="160"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="24618" checksum="E4B7CAC406CE1E11" modified="2017-01-18" version="1">MKLSVRTSNENTLSLTVPDNATVEDLKIAAIVALPPATRGAPHLTPSCINLWYSGHKLDYSKPLRSYNITPDSVVANPNLVVYLTLDVTSSEGSGDGSPTMPVSPQVLAAAATAVASLASAGPADSAILDDNEEADQDDLLLQQQKKTKKSKSKSKSRSKSKSKKCTFSNCTSAPLRMVGDCQFCQGKFCSKHRLLESHNCKGLKICKDKCYERNALKLQSEQTVASKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRQ6</accession>
    <name>A0A1E3NRQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_177996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019879.1">
        <property type="nucleotide sequence ID" value="XM_019161749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRQ6"/>
    <dbReference type="GeneID" id="30178436"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="408" mass="43380" checksum="319347EC0B4E8212" modified="2017-01-18" version="1">MKPLSLPSQGPPRLPVRPPPSPREDAPVSGPEPVASGGEENRAATTTMRLLSPQAGAPSAGSGGSGPDAGTEDDPEYDTGDGTDGQAPTQTEVPVLPKRPALLTDLSSPEDYYREEENGFANLSEFFFLNPVSGTSTGTTSIMDNPPRQLQPLSLTLPSITSVANKGIYSAGHGTASTGHASNGSSSSNGSNGSNNAGKGSGSRKSNPGIPSLNGSDSGSNHSNSNSSNHSNSSNHSNKFYIHLLEDVHLDDQTVLFDLVDTLMRVRSENEELKEGLWDKIDSLSTATRKKAGPGRPDDAARYDHNSLLFDSKLTNEQLKQMLDEQRTYNGVLAQTVDEFQDSLDTIMDSLVGSNQDAGIELLSAIRKGEAVVEENSDEMWKSWMEVVSALDDVAKFDRAIVKTVHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRZ8</accession>
    <name>A0A1E3NRZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_29517</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019927.1">
        <property type="nucleotide sequence ID" value="XM_019162596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRZ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRZ8"/>
    <dbReference type="GeneID" id="30179283"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070199">
        <property type="term" value="P:establishment of protein localization to chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031107">
        <property type="term" value="P:septin ring disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031134">
        <property type="term" value="P:sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000016">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="515" mass="60808" checksum="14A5DD3093EF1630" modified="2017-01-18" version="1">MIHKQNRADDAPANLSDQLQPQGEIDFLKYHTSHSPFDKVPKDDLELKPKTPQRHSSSRFEPSLNNQAFEKSPPFEEVDPQFHIDLFIQKVDQCKIMFDFYDPSSDIQGKELKRITLHELTVFISTTRINFTEEMYKHVVDMFKINIFRPIPPPVNPIGDIYDPDEDEPVYELGWTHMQLVYEFFLRFVESPDFNNTIAKPYIDHKFVLTLLDLFDSEDNRERDCLKTTLHRIYGKFLSLRSFIRKSINNIFLTFVYETGHFNGISELLEILGSIINGFALPLKEEHKIFLIRVLVPLHKARSLSLYHPQLAYCILQFLEKDSSLTEEVIMGLLRYWPKINSPKELMFLNEIEDIFEIIDPNEFQKIEIPLFAQLSKCISSPHFQVSEKVLCFFNNEYFVSLVTENAENILPIIFPSMYELAQPELDILENQNQSEVDFDEPNNWNRTISTIAYSALKIFMDTNPVVYDRCFVLYEQQIKEDNERKHLREEHWKKLQEYVDKLKAGERERDVEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS14</accession>
    <name>A0A1E3NS14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="3">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14367</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019947.1">
        <property type="nucleotide sequence ID" value="XM_019160339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS14"/>
    <dbReference type="STRING" id="763406.A0A1E3NS14"/>
    <dbReference type="GeneID" id="30177026"/>
    <dbReference type="OrthoDB" id="9991235at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000392">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000015">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010568">
        <property type="entry name" value="PRK13961.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="11">
        <location>
            <begin position="15"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="300" mass="33442" checksum="C8FB928F2FD24FE9" modified="2017-01-18" version="1">MSITSTNLDGILPLIAKGKVRDIYEVDPSSLLFVATDRISAYDVIMENGIKDKGKLLTQISIFWFDFLKDNIKNHLIASTDEEIFAKLPKELSEPKYKNQLKGRSLLVRKHKLIPLEAIVRGYITGSAWKEYQKSGTVHGLPAPEGLLESEVLPTPIFTPSTKAEQGEHDENISPAQAAKIVGEDVCEKVSEKAIELYTKARDYAKTRGIILADTKFEFGITDDGELVLVDEVLTPDSSRFWNAKTYVVGKSQESYDKQFLRDWLTSNGLNGKDGVSVPDEIVEQTSAKYAEAVDALTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS15</accession>
    <name>A0A1E3NS15_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70129</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019611.1">
        <property type="nucleotide sequence ID" value="XM_019163566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS15"/>
    <dbReference type="STRING" id="763406.A0A1E3NS15"/>
    <dbReference type="GeneID" id="30180253"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="221" mass="24543" checksum="947DD79B454364A6" modified="2017-01-18" version="1">MSKADEYGYDYEFLFKIVLIGDSSVGKTNLLSRFTRNEFNPDSKATIGVEFATRTLEIDGKKIKAQIWDTAGQERYRAITAAYYRGAAGALVVYDITNSDSYENVSKWLKEMKDNADSNMVIALVGNKSDLSHLRAVPTDEAQSFATEHSLLFTETSALNADNVDFTFIQLVQNIYEMVSKTKFDINDENGANGNDVAKGKTIQLTPAPKDQKVSKNSNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS47</accession>
    <name>A0A1E3NS47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70492</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020012.1">
        <property type="nucleotide sequence ID" value="XM_019163655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS47"/>
    <dbReference type="STRING" id="763406.A0A1E3NS47"/>
    <dbReference type="GeneID" id="30180342"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000549">
        <property type="entry name" value="ABC transporter ATP-binding protein arb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000618">
        <property type="entry name" value="ATP-binding cassette (ABC) transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF15">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="77"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="388"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="605" mass="67777" checksum="B0451E8C2562C15D" modified="2017-01-18" version="1">MSVSASKAKRDAKKAAKLADGKMKKLTKKEKEKLENQDEGDSAADAIAKLKLQQDKDGLSDRVTTGVLESLVTSRDIKLSSVSLLFHGKVLIQDSTLELNYGRRYGLLGENGCGKSTFLRALAAREYPVPENIDIYLLNEPAEPTEYSALEYVVREAEAALAAMEKEVEDIIVEDGPESLLLEPLYERIDEMDPSTFEARAAIILTGLGFNSKTIKKKTKDMSGGWRMRVALAKALFVKPTLLLLDDPTAHLDLEACVWLEEYLKNFDRILILVSHSQDFLNGVCSNMIDMRMKKLLLYGGNYDAYAKTREEQETNQMKQYNKQQEEIVHIKKFIASAGTYANLVRQAKSRQKILDKMEADGLIEPVAQDKVFNFRFPEVERLPPPVLAFDDISFAYDGKPENNLYDNLSFGVDMDSRVALVGPNGVGKSTLLKIMTGELQTQKGRVSRHTHIKLGVYSQHSADQLDLTMSPLEFVREKYSHISQDYQYWRGQLGRFGLTGEGQTSQMATLSEGQRSRVVFALLALEKPNILLLDEPTNGLDIPTIDSLADAINEFNGGVVVVSHDFRLLDKIAKDIFVVENKTATRWDSSIAEYKKKLAKNVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSF3</accession>
    <name>A0A1E3NSF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase II subunit A C-terminal domain phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14571</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This promotes the activity of RNA polymerase II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020200.1">
        <property type="nucleotide sequence ID" value="XM_019160439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSF3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSF3"/>
    <dbReference type="GeneID" id="30177126"/>
    <dbReference type="OrthoDB" id="10249888at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17729">
        <property type="entry name" value="BRCT_CTDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039189">
        <property type="entry name" value="Fcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011947">
        <property type="entry name" value="FCP1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02250">
        <property type="entry name" value="FCP1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081">
        <property type="entry name" value="RNA POLYMERASE II CTD PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081:SF36">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="9">
        <location>
            <begin position="166"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="8">
        <location>
            <begin position="595"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="408"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="732"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="734"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="774"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="792"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="816"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="836" mass="94134" checksum="9FBC0EF1810FB465" modified="2017-01-18" version="1">MSDYESDCTRVYLPNSFPYPLTVLKVNVKEGDELAKYSPIITYKYYDYEPVPISEQDEEQTDESERRLVKVENVGTFDSTVNGKVAKVYVSVNDEIKDSQALIVDVMEPCSHPIQYGGLCAVCGQPVEEEEDNRRAPISMAHGTTDLKVSSREAESIERSSTDRLLKEEKLSLVVDLDQTVIHVTVDPTVAEWMSDPTNPNYEAVKDVRSFILEEPAITPFGYKGPMLPPTKRWYYVKLRPGLQDFLLKMSKRYEMHIYTMATRQYAESIAKIIDPDGIYFGERILSRDESGSLTQKTLKRLFPVDTSMVVVIDDRGDVWNWSPNLIKVVPYDFFLGIGDINSSFLPRQQMLLGPSKRRKSVNILEEQLSIEGETGAKNEGVTGGEEGQKSDDIQAKEKVIELSKLREQTEEAVIDDNETSEPQLNGDTQTSETDSTSINDEDSKNKENTTQDIVDKASSSPVDRLVELSFAGDNSNLLAVQENERTSALEQQEHDRPLAKLQENLDKIIEEGEDEAAIDFDNETEKEKKLNAKYKSMVDSKEAHNLLYDDDKELIYLGQALVRIHNEFYFEVEKFRQELGQGTLPDVKDIMTEMKSIVFQGCVFLLSGVLPLGTPLKNADIVIWAKSFGATFVENYNPKVTHVICKNGGTFKVRLAKSVDSNVKIVNPDWLFACMSLWDRISEEPYEVKVDSLLSKAQVDDFLQFHGDDAALESKSLDWGEIDEEMKEFMGSDYEDDDDDDDDDDDDDDDDDGDVDDGAATTADDENSKTALESNAASDEDASASAKSGKHTLEDDLDNNKRQKVTDEPKGQAVGEDESEDEFERDLLEHLADLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSH9</accession>
    <name>A0A1E3NSH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="14">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_70159</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="14"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019646.1">
        <property type="nucleotide sequence ID" value="XM_019163574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSH9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSH9"/>
    <dbReference type="GeneID" id="30180261"/>
    <dbReference type="OrthoDB" id="2748310at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000015">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 14" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="504"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="528"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="590"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="620"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="678"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="711"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="761"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="833"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="864"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="903"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="15">
        <location>
            <begin position="453"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ48533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="974" mass="111611" checksum="C27F4E1256B0B628" modified="2017-01-18" version="1">MADQKSVHSKRSILLGVGVLFHLIYLWSIFDIYFVSPLVHGMDQHKSTASAPAKRLFLIVGDGQRADKTLGKIYHPRTKKEEYLAPYLRSIILNEGTYGLSHTRMPTESRPGHVAMIAGFYEDVSAVTKGWKENPVDFDSVFNQSTHTYSFGSPDILPMFAQGATEGKIDTWMYGHEFEDFTSSSIELDKFVFNHFYQLLENSTTNAQLDEEIRQDGNVFFLHLLGTDTSGHSYRPYSAEYYDNIQYTDAEISKLVEKVHEFFGDEETAFVFTADHGMSDFGSHGDGHPNNTRTPLICWGKGCNKPVRIDPSSNDFLRDKFESDDMANWDLDDVKRNDVKQADIASLMSYLIGTNYPMNSVGELPMAFIDDVESNKVKGLYYNSLTLLEQYIVKLHEVKDNQFNFKGFSKFEDKSVESYKANIEKVIKLMDVNTELESEAVLLIEEFNSVILEGLDYLQKYNWLMIRTIVTFGFIGWIVYSFTIFLQMFIIPHEKLFRYEGMHLVRKFFFISFTSVMLYIFQYENLPLNYYFYLVFPIFFWYNIFKYKTDLVKGFNIFFKGLSNIWKVLIFFGIVVFFESIAVGFENREVFSYIYLILSASYPVLLFQKSLLQNKKHLKISICWFFTCISLSIFPIQNPVKVESINLIVTSGLIMTGIGILGLTFLQKKMSEKTRMIIGLQIVLIVLSIYSTLKAVVSLTNRKGLPLDAQVIGWTVMIASLTVPTMLHYIYPNSNYQIRFLVIFLTLAPTFNILAISFEGLFYILYSLMLILWIEMESMSTKSNNNLRLLRISVIGFFNLQISFFGTGNVSSISTFSLDSVYRLLPIFDPFPMGALLMLKLIIPYALLSTGLGLINLRLGFSKYSVTSLIISVCDLLSLWFFYMVRTEGSWLDIGVSISNYCLAIFGALFIAIVEVGSGIVLSGVHIEEHKEAKAVAQLKMDDKQYHEIEKIINDDQLDNGTIGSRVRRRVQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSJ4</accession>
    <name>A0A1E3NSJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating</fullName>
            <ecNumber evidence="13">1.1.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">C-3 Sterol dehydrogenase ERG26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16 17">C-4 decarboxylase ERG26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Sterol-4-alpha-carboxylate 3-dehydrogenase erg26, decarboxylating</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_14559</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4beta-methylzymosterol-4alpha-carboxylate + NADP(+) = 3-dehydro-4-methylzymosterol + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:33447"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:50593"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64925"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33448"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.170" evidence="13"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020184.1">
        <property type="nucleotide sequence ID" value="XM_019160433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSJ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSJ4"/>
    <dbReference type="GeneID" id="30177120"/>
    <dbReference type="OrthoDB" id="10058185at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102175">
        <property type="term" value="F:3-beta-hydroxysteroid dehydrogenase (NAD+)/C4-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09813">
        <property type="entry name" value="3b-HSD-NSDHL-like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000346">
        <property type="entry name" value="C-3 sterol dehydrogenase/C-4 decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="20">
        <location>
            <begin position="10"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ49071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="350" mass="38861" checksum="20A9A97EE7192D77" modified="2017-01-18" version="1">MATPLESVLLIGGSGFLGLHLIQQFWEESPRPKIHVFDIRPLPDKISKYFTFDPKQIAVHVGDLTSEDDVKKAIESSKPSAVVHSASPIHGLGQELYEKVNVRGTANLIKCCKETGIKALVYTSSAGVIFNGNDVHNADETWPIPEVPMDGYNETKAIAEEMVLKANSPEEEFYTVALRPAGIFGPGDRQLVPGLRQVLERGQTKFQVGDNNNLFDWSYAGNVADAHVLAAKKILDPTTVSSVGGEKFFVTNDTPSYFWTLARTVWKADGHVDKYNIVLNRPVAICVAYLSEFFSKIVGKEPGLTPFRVKIVCAYRYHNITKAKTILGYKPKVGLEQGIRYTLDWMDETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT17</accession>
    <name>A0A1E3NT17_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_21834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020307.1">
        <property type="nucleotide sequence ID" value="XM_019162224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT17"/>
    <dbReference type="GeneID" id="30178911"/>
    <dbReference type="OrthoDB" id="4084551at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028000">
        <property type="entry name" value="Pma1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14610">
        <property type="entry name" value="Psg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="341" mass="39030" checksum="749E46E3FDA15940" modified="2017-01-18" version="1" fragment="single">ETQRPWIRTIYGSVKEVVTPYVVGGVTFAAQPPATADGLEPWISIKNDGLPKTITPKMKNNVIENSFPAVRTFFQTATTVVHHQNEIQAHNLADGETVEEVIMIDEDDTYAKLSPIQRCTPDYYYKKGIANMDSSEPFCSPRDHQKMRAGATYFITWYSRFFKKAKNVRFHYAFVNEKSHDKGFDKRDLMDSAVKKVEAEIEELSGNVDFSGDVHGAFFSSDWVHNDNGWFAVEVDKKWLRGKVYKKVVIAMQPDNVADEDFSILNAPHIFATFQLRESIGKNTKEMRKIQDKAGTGDDVYYVIASIPTVVMISVLFMYGFLYINRRHRDLSKVKKPKRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTA1</accession>
    <name>A0A1E3NTA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14771</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020415.1">
        <property type="nucleotide sequence ID" value="XM_019160524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTA1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTA1"/>
    <dbReference type="GeneID" id="30177211"/>
    <dbReference type="OrthoDB" id="512667at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="6">
        <location>
            <begin position="76"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="204" mass="23047" checksum="4CE23444E8BD92B1" modified="2017-01-18" version="1">MLRRILKLDNVVPKRLGGLIPSPRYFTSYYPIRNAEDQSDGGHSHTHNGTPCSGHTHNHNHSHNHAGIADRNTLKMDKPMLMLAFTCKKCDTRSSHVMSKQAYQHGTILVQCPGCKFRHLVSDHLKIFSDHRIKLEDIMKAQGENIRTDTTDLVFEDIPESLKKLIGHHAKNAPAEYKKEIEFSEETPKLSSKADEGDSNKATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTA8</accession>
    <name>A0A1E3NTA8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020397.1">
        <property type="nucleotide sequence ID" value="XM_019160515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTA8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTA8"/>
    <dbReference type="GeneID" id="30177202"/>
    <dbReference type="OrthoDB" id="3998102at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="219"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="410" mass="42555" checksum="725B377F53212679" modified="2017-01-18" version="1">MSDQEVFDALQQARNLNDDQALQLLESTIHKCEQSNVDSNLFCKVRILKGDLLLKKGDVQSAVSEYQKASELDERDGKIDSPELKTKLDSLINNVTSQLQRNVSENNTSSNERDLSSLLLGQIAGASGSNSSKLSLLSTLMSSGGGSSNSGSGGFMSLVQNLASSGANANSQKASGGFNVSSLLSMASGSGLMGGSGNNHGSGPVAGASMGSLVGMLMGSGSSHNNNNNNNGFNNSNSGYNSGYNNSNNNNTGYNNGNSNSQFQSHSGNNNNQNNYSNENGYGHNNYNNSQGGFNNGYNNGGNYGNNQNNNSNSYPDQVNSYNNSGSGYSQQQQGGGVGASLMHMAENYMSQNSEGSYGHNSNQNHGGNQGYGGNQGYGGNQGYGNGNSGNGNSGSGTASTLMNLANKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTB5</accession>
    <name>A0A1E3NTB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 12">Vacuolar protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_70811</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Vacuole membrane</location>
            <topology evidence="1 12">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020369.1">
        <property type="nucleotide sequence ID" value="XM_019163747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTB5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTB5"/>
    <dbReference type="GeneID" id="30180434"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071563">
        <property type="term" value="C:Myo2p-Vac17p-Vac8p transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071255">
        <property type="term" value="P:Cvt vesicle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051656">
        <property type="term" value="P:establishment of organelle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061724">
        <property type="term" value="P:lipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071562">
        <property type="term" value="P:nucleus-vacuole junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903044">
        <property type="term" value="P:protein localization to membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000131">
        <property type="entry name" value="Vacuolar protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045156">
        <property type="entry name" value="Vac8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0926">Vacuole</keyword>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="86"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="168"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="209"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="252"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="293"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="11">
        <location>
            <begin position="334"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="536" mass="58908" checksum="1CA76AB5D761DE44" modified="2017-01-18" version="1">MGCSCSCLKGHEDSGLYRQLLADNEREAIASLLEYLENRSGVDFFSEGPLRALSTLVYSDNIDLQRSAALAFAEITEKEIRPVTRDVLEPILILLQSSDQEVQRAACAALGNLAVNDENKVLIVEMGGLTPLIRQMMCPNIEVQCNAVGCITNLATQDENKTKIAKSGALIPLTKLANSPDLRVQRNATGALLNMTHSYENRKELVEAGSVPVLVQLLSNSDPDIQYYCTTALSNIAVDEENRKILSTTEPKLVSQLVQLMDSSSTRVQCQATLALRNLASDALYQLEIVRAGGLPNLVQLLQSPHEPLVLAAVACIRNISIHPMNEVLIIETGFLKPLVDLVDYPNNTEIQCHAVSSLRNLAASSERNRMALLEAGAIKKCKDMVLNAQENVQSEISACFAILALADELKEKLLDMGIIDVLIPLTKSSNAEVCGNSAAALANLCSRIKDYSVILEHYDDLSDFISNFLKSRNPTFEHIALWTILQLLESNNKEIKDKIKKEISKGKIDLTNLTGENNENHEISNLTMQILDLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTD0</accession>
    <name>A0A1E3NTD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_29304</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020482.1">
        <property type="nucleotide sequence ID" value="XM_019162586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTD0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTD0"/>
    <dbReference type="GeneID" id="30179273"/>
    <dbReference type="OrthoDB" id="245697at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13945">
        <property type="entry name" value="Chs5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.200:FF:000002">
        <property type="entry name" value="Probable transcriptional regulatory protein CLM62_37755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031673">
        <property type="entry name" value="Chs5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052827">
        <property type="entry name" value="CHS_Export/Cell_Fusion_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031669">
        <property type="entry name" value="Fn3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048300">
        <property type="entry name" value="TACO1_YebC-like_2nd/3rd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049083">
        <property type="entry name" value="TACO1_YebC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351:SF1">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16892">
        <property type="entry name" value="CHS5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16893">
        <property type="entry name" value="fn3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20772">
        <property type="entry name" value="TACO1_YebC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="YebC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="76"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="4">
        <location>
            <begin position="164"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="310"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="409"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="437"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="473"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="494"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="521"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="88276" checksum="7CA4955DA3994BC2" modified="2017-01-18" version="1">MVEVSLTVGKLDASLALLLTKDHHLIEFPTILLPDGISTGSIVKITCDRDLQKENEEKKAFNALQEEIFEIFGKHEPQNPLLKIVNITQTSCVLEWEPLNLGTAELKELTLYKNGSKLGPIKNPFQKKNIKFSGLPIDTPYKFQLKLETSSGIYYSNLVELRTHKMTDLSGITLCIGDIDFNEEKFSLNDIEDAIKAINAKPFSSQVKVDTTQFICTTNTGIEYQKAKNMNIPIIRPEWIKACQLERRIVGVNKFYLDTENPIWKTKDFWAISNDVTPPTEDTEEPAIAANSTTVEEDLTVPNITIDPESEAEVTVTDPTSESTNTNDVAAVETETDKNDEKIKDSEPESLPKIEGETVPEENSMTEEVDEVPKEEIEGAKAEVAAAEVAPAETPGVLAEEGVVVEETTATPELAVTQDESPAVAEEELGQSVTVNESKEVEGGKEAKDDVTETAEPSIAIEEPTEEVKISLIDSSPATPEGTNAEASATEIGSEPKREGEENGVEKVQAETKSTPSSKGSNKKKGKKKGRKYAKRRLHTSDVRMAGHSKWANIKHKKAANDAAKAASSFRMSRQIQVLAKMGGSDLNQNIMLASAIDKARGMCIPKKVIDNAIKRGTGELKSTEKMETVVYEGLGPGGVAIVIEAITDNKNRTIGYIRPCFNKFNLNMTPTLYMFEKKGVILIDAGELTSDDLFDELLELGCEDINDIENPNESELLSEKKDGNIIELITDAKEFGKIANELKDKYPIKEMNIEYLPNEETLVEVSDPDTRASYEKFMGLLDNIEDISQVFTNLKETEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTH7</accession>
    <name>A0A1E3NTH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Ribonuclease H</fullName>
            <shortName evidence="11">RNase H</shortName>
            <ecNumber evidence="4 11">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_71001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="4 11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020570.1">
        <property type="nucleotide sequence ID" value="XM_019163796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTH7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTH7"/>
    <dbReference type="GeneID" id="30180483"/>
    <dbReference type="OrthoDB" id="407198at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09280">
        <property type="entry name" value="RNase_HI_eukaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.970.10:FF:000002">
        <property type="entry name" value="Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050092">
        <property type="entry name" value="RNase_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017067">
        <property type="entry name" value="RNase_H1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642:SF30">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036852">
        <property type="entry name" value="Ribonuclease_H1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="13">
        <location>
            <begin position="115"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="48"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="274" mass="29600" checksum="30AE412750FD0CC3" modified="2017-01-18" version="1">MKYYAVRNGRASGVYNTWSDCESQVKGFAGNQYKSFASREAAEAYVKGGAVSSGRTAGPVRGRVSGNTRPSGSLPSRSSSSRHSTSYHPYPKPPSCTHIKPSLPVAYVPPPPPTALERVEVYTDGASKNNQARKQGLSKAGLGVYYGPNDSRNFSGRVTGEQTNSRGELEAIQHALQNSAREAKHGNPQLTVIHTDSQYSMDSIQKWSKKWETNGYKTSSGADVANQDLIKSCRRLVNEIEANSGSVEFVKVKGHSGNHGNDMADRLAVEGCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTJ8</accession>
    <name>A0A1E3NTJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Mediator complex subunit 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_14854</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the SRB8-11 complex, which itself associates with the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020497.1">
        <property type="nucleotide sequence ID" value="XM_019160566.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NTJ8"/>
    <dbReference type="GeneID" id="30177253"/>
    <dbReference type="OrthoDB" id="103819at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009401">
        <property type="entry name" value="Med13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051139">
        <property type="entry name" value="Mediator_complx_sub13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021643">
        <property type="entry name" value="Mediator_Med13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249:SF3">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06333">
        <property type="entry name" value="Med13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11597">
        <property type="entry name" value="Med13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0010">Activator</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med13 N-terminal" evidence="13">
        <location>
            <begin position="22"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="12">
        <location>
            <begin position="1246"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="353"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="698"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="796"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1585"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1644"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="809"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1644"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1654"/>
            <end position="1663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1690" mass="191483" checksum="2C977FD11487D685" modified="2017-01-18" version="1">MPNNSDFATALKKKKVLKKLVPYSILKISPSLNQKNELIINMTSLKKSFYRLTDFITVTCNGSIKTLDEKSKFALYIAPSGIRCLIAESSYSDSITDEPPENYEKLFNILKTFNDIDLSHNSSPINDKRLWIKIHPIGFSSNNTAPVIANYLANTSSTSKKFIYWPLELCFIQFSSDSVPGASANDDFELDTFTIKDPFQVIDEFVDILEDIEKTKVTEEHKGSEQEVDNGNGDQQDGETSTLMSASKQIDPNFERPDNRSELFPVSFDFKPELDSLDQQLKPLKSMNDSGIDNINDLMRVNDGFDDLFKGKGDDDMGLMDPSQQRDEHDFQKELRNVFRNDETITGVISVNKGDQDSISPEKKVNPTFEEDWGDLFGDSAEDDENDDNDSEQKNIDREVDQELAELENLEDLNYNGDIITFPTKNETEHIDLLDDLAERNNTKPQVDETSPHNFEDMREDDQEGVSSMKNEQPTFFPIRKASTMSPHDPAGQLLAPPRILDTSPLYQDPGAPSPIPFQIFAPPVYSSEDSSPLPTEEKTVLKPFSSLTPKEKKKSVFSPLNFNPLIEKDIDSKYSNGGKFFVKKSSSVSGFPASDPLDPKYIDSFSDGSKNLMHPTAKSTTPVLEGTFNHISSLTTPSFLVRGPGKNTDGMVLAADDLISDSDDEMSEVADDSKDEIMLYDIGGLSQVDGEVVNTGIMDNENEKPDDMDDENEQDEKNSSDLSADVGFVGQKGAIGFGIATPGADGNSSKRRKLETIFDNINDDDDFDFDFDDDYNDDKDYTGDDDDIDALHMDETKRPTSSDPEVSNDARLDTEASRERGINDVSGIEIPNSWFYVLRPIAPIQIPFNFLQSVKLTVEASKILLLLPILQEYVLFSQKYMNNQMLNMVVQSRECPSVLDSDVEYLLYKIFPGICKVNCYELIDSDKESKQIKPFECLFGQNLNRERKSITSRNKPDTELKIFDENNYESSPMLNVPFPDSQQNHNVPQYMEDFNEEFQKIDLTETISTDNLFRINPTVLSFKRLSQDVIINKIGINFWKMLNLEPPLSTKDFGILFVIPKANIDFRNRAEHFLEGIISYYKGCKLGEIRKLIVSGLIEVPYDTGNEAHYWDYVQTQLLSIVEELQNQLTLDERGKVLLMFVDPFQDLNSLIRMADVTQTFENSLVEKHVSDGETSKKKKKRKNKAIARLPITLFYKAFSVASFYQRDDGQFVIFTEKDYFNLVLELYNNCPNDGSQMILKDRSSMFVIEKDIQKTVKFMLTRKPVSKLIENETFLHLCYERSVDKKWCVASWISQCGKLNYTKSWYIDDMVEGSQNFEKVADDIMEITMDYVSHISGKCFVILTRLNNIIPDDELAEWKRLSMKNNDLILVVMTAEIESSTLILSNNPGHVLSPKKKEETSKDLNYTVASTNSQSQTPGNMLLNSNNYAHQLAGSTRFESPDVSMYTPSYDAALSPMDISTHQVQVPIQTLQPLLEDNPVQDALSQNPILIDISDECYGLILQVSQPLANQPRVPLKTGFLINTGEGATKNRVLEINLLSCQSGISITKFLKTLLVQYRNLSSMAPYFGVSGILRHCGNVDCDESGEEEDDEDAGTGWEDENLNSSSALGISGSGQNFGNDRVERKKERHYIQQQYLQFHRMQQRQKRQRAKERERELSESDSHYNVIPVHMLAVRKMLDFLVNIQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTN2</accession>
    <name>A0A1E3NTN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14901</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020557.1">
        <property type="nucleotide sequence ID" value="XM_019160588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTN2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTN2"/>
    <dbReference type="GeneID" id="30177275"/>
    <dbReference type="OrthoDB" id="10248581at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="31"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="124" mass="14438" checksum="616494635D9DE7EF" modified="2017-01-18" version="1">MNAPDRFELFILPDGVPKIQITPDSRVPNCLLIKFEREDHTLGNLLRDELINDPKVLFAAYKIEHPLFANFMMRLQTEEGYNPRTALKNACNRLIQRLGLLNEKFKREWELKALLSATENDDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTS4</accession>
    <name>A0A1E3NTS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_91503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020191.1">
        <property type="nucleotide sequence ID" value="XM_019165071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTS4"/>
    <dbReference type="GeneID" id="30181758"/>
    <dbReference type="OrthoDB" id="10660075at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="464" mass="50053" checksum="36DDD2078807F0A5" modified="2017-01-18" version="1">MSSLHHLARHNAEPAAGAPAPAPRSRKHTVLANFKSISRRKKLLPQTQQYAAPSPRGPDHTQHPPPLSRRPSTASAAAAAIPSTETFNNLAQLSNNPALRTRNPARKRHSVIIHSVTEEESRKNSDPHNPLYVGHSSRHARSTSSLDDLNLGDDGANNRPSLSNGDDLDADSDLDDDDDDDDLDIVHVSQDSDTILRDLDPGTGLDGSHSSKNYSNTLNNAFSNYPSQISAIPASASVPASLSSRASLTSSISTANSPSSSSRQKYHPLRKLVINSPNNPSSSTFSKNTITPVSTASISTANSSKTQVPPSSSPSPSPSPSPYRRLDPNSIQNRANFIHQQHKNSTTAASIPSLHIFQPDLSDEKPHIVPVSNCLFFETLKPQSLSPAKPSQTLAADVFDDFDTSPTKKKSFPFQRIHYSHQNTLLSTFAKGLVDPDLFSTNASSVFSGPKALPSKHRTSLYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTY8</accession>
    <name>A0A1E3NTY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="9">DMRL synthase</shortName>
            <ecNumber evidence="4 9">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_57487</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homopentamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 9"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020569.1">
        <property type="nucleotide sequence ID" value="XM_019163241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTY8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTY8"/>
    <dbReference type="GeneID" id="30179928"/>
    <dbReference type="OrthoDB" id="2965at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.960:FF:000007">
        <property type="entry name" value="6,7-dimethyl-8-ribityllumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="158" mass="17568" checksum="B5CE6B08B92C383B" modified="2017-01-18" version="1">MAVKGLGKVDQQYDGKDVKVGILHARWNNKVIDALVAGAIQKLKEFNVREENIVVESVPGAYELPYGTKKMFEKHGVDVVIPIGCLIKGSTMHFEYICEAVSTQLMQLQFQLDKPVIFGLLTCLTEEQALLRAGVLPGMHNHGEDWGACAVEMVLKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUB1</accession>
    <name>A0A1E3NUB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial import inner membrane translocase subunit Tim21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_26019</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TIM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020699.1">
        <property type="nucleotide sequence ID" value="XM_019162427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NUB1"/>
    <dbReference type="GeneID" id="30179114"/>
    <dbReference type="OrthoDB" id="436405at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.320">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013261">
        <property type="entry name" value="Tim21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038552">
        <property type="entry name" value="Tim21_IMS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032:SF6">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08294">
        <property type="entry name" value="TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="179" mass="19957" checksum="F0DB5303B69DCF02" modified="2017-01-18" version="1" fragment="single">VKAGSRLLLSSWLIIIAAGATLVTSYLVATEIFSPSGETSTFNRVVNMIEKDENCLRLLGYSDSEIEQGGIRLKAYGGMSPDLWTRNIPVQATKFSGKDQKEHMIMRFFVESNRTVGVVNLEAIEENFINQELVYVTIDVKGHNRHYLIGGPHLSYAQKNLNPFSSQGFLGVKWGSKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV62</accession>
    <name>A0A1E3NV62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prenyltransferase alpha-alpha toroid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70979</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020546.1">
        <property type="nucleotide sequence ID" value="XM_019163788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV62"/>
    <dbReference type="STRING" id="763406.A0A1E3NV62"/>
    <dbReference type="GeneID" id="30180475"/>
    <dbReference type="OrthoDB" id="24893at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="8">
        <location>
            <begin position="13"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="367" mass="41715" checksum="B036C5431025F4D9" modified="2017-01-18" version="1">MVENEMKGKVYNLDVKKHISCHKRFLSLLPSKFESEDSNKLAMAYFSLASLELMGTLHDSFNDFELQEFVDYIYSHLIESEQVSGFRGSLTYLKRVESIDLASTCFALQCLLILGDSLGRIDKKKVLFFVQSCQKNNGGFTNTLDSLNAKDLRYCMIAATVCKILCKDFPNYSQAIDTESLKSFIYGLQTYDGGFSMCKGDESHGGMVFCAIDTLSLIDENLRRSIHLDKASMLLEFLVHRQVHFGKYNEQEYLQNENADMRDNGGFNGRLNKYADTCYVFWTLASLELLGFTHLIDEKAAINFLVQNTQNIYMGGFNKTTDEDEFPDPFHSFLGLSALSILKYPGIQSLNCQLVIPQSSYVHWKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVF9</accession>
    <name>A0A1E3NVF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="6">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71078</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="6"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020646.1">
        <property type="nucleotide sequence ID" value="XM_019163823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NVF9"/>
    <dbReference type="GeneID" id="30180510"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000263">
        <property type="entry name" value="MAP kinase skh1/pek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF6">
        <property type="entry name" value="MAP KINASE KINASE MKK1_SSP32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="235"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="620" mass="69278" checksum="3462C28C233AF321" modified="2017-01-18" version="1">MELQPPHHRQQQKQQVQMQQQQQQMPGPQQPETPTTAALPRLRFSRGGRKKPAPKLNMSIPVESPTAPLNIFSNKNLPSLPPMPKQFKNSQTIDGSKNDKPTLNLVDSGNSNGNGNGTSSSINSHHSSNSNNSNGNNSSNTLNEVGLNGLPSAATTLTVASISTLNDENLPLDRSPCLISAFSNKLKISENEDLANTSSDSYRTNTPTESDIILTKPLESLTEEDWKVLHERELIETLEVLGEGNGGSVKKCKTLTKNPAIFALKIITTDPSPEFQKQMVRELNYNRKFDSSYIVRYYGTFLDESSSSIHICMEYMGGRSLDAIYKAFKERDGRLSEKPLGIIAGSVLKGLAYLNEKKVMHRDIKPQNILLDLQGNVKLCDFGVSGDVVNSLATTFTGTSFYMAPERIKNEPYTISCDVWSLGLTVLEGALGEFPFVRESSPDLMISPLDLLMIIIEFEPILEDEPDEGIKWSKGFKDFIKVCLTKQGRQRPSPRQMLEHPWLVKMMNKHVRMDKLVENVTSYRAILNTTQLALCLNEAGTRSVKFYTFNETRSDYVVVAIDCDGNLMNQFDCLEQQDLSLLGLYRGEEIEGATQLYDEILTWTGEFHGHLDDESRNVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDQ5</accession>
    <name>A0A1E3NDQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Iron-sulfur cluster biogenesis chaperone, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_37562</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the assembly of iron-sulfur (Fe/S) clusters in mitochondria. Assisted by the DnaJ-like co-chaperone jac1 and the nucleotide exchange factor mge1, it mediates ATP-dependent Fe-S cluster transfer from the scaffold proteins isu1/isu2 to grx5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015357.1">
        <property type="nucleotide sequence ID" value="XM_019163089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDQ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDQ5"/>
    <dbReference type="GeneID" id="30179776"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000020">
        <property type="entry name" value="Chaperone protein HscA homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000007">
        <property type="entry name" value="Heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="628"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="648" mass="70381" checksum="962A6CC2C821145C" modified="2017-01-18" version="1">MLANVSKNIRSVRFAQAPKFVRYNSNKVQGTVIGIDLGTTNSAVAVMEGKTPKIIENAEGSRTTPSVVAFTKDGERLVGIPAKRQAVVNPENTLFATKRLIGRRFEDPEVQRDIKQVPYKIVKHSNGDAWVEARGEKYSPAQVGGFVLNKMKETAEAYLGKPVNSAVVTVPAYFNDSQRQATKDAGAIVGLNVARVVNEPTAAALAYGLEKDDSKVVAVFDLGGGTFDISILDIDNGVFEVKSTNGDTHLGGEDFDIALVRYIVDQFKKESNINLEGDRMAIQRIREASEKAKIELSSALSTEISLPFITADATGPKHINLKMSRSQLEKLVEPLIKKTYEPCKKALKDANLATSEIGEVILVGGMSRMPKVIETVKEIFGKEPSKAVNPDEAVAIGAAIQGAVLSGEVKDVLLLDVTPLSLGIETLGGVFTRLIPRNTTIPTKKSQVFSTAADGQTSVEIRVFQGERELVRDNKLIGNFTLAGIPPAPKGVPQIEVTFDIDADGIINVSAKDKASNKDSSITVAGSSGLTDSEIEKMVNDAEKFKEQDQARKEAIEQTNKADQLCNDTEKSITEFGDKLDKADADNVKKLITDLRDLIVKVNGGENVEVADIKAKYEELQQSSLKLFEKMYKNQQPDADSNKEEPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDU8</accession>
    <name>A0A1E3NDU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Elongation factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Eukaryotic elongation factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_37379</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015428.1">
        <property type="nucleotide sequence ID" value="XM_019163080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDU8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDU8"/>
    <dbReference type="GeneID" id="30179767"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008079">
        <property type="term" value="F:translation termination factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000002">
        <property type="entry name" value="mRNA export factor elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056891">
        <property type="entry name" value="NEW1_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24988">
        <property type="entry name" value="NEW1_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="485"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="727"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="12">
        <location>
            <begin position="858"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1057"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1095"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1114" mass="124365" checksum="EDA473980CF4A503" modified="2017-01-18" version="1">MSYEEFLAHQQSSLNSRPNVPSGQSSQVSSRANSNSTVPNVSSSPMSANASSTSISSALEKLEITPLSASLSELNKSTKISEVREQCNTIADLVYEHGASSLQDWKIGEVIKTLSKPKNSPLLREAAPVLIQTLSRKFAGATPNEAYFVDFFNYAFDLFTDKENTVKRAAQAAVDTLYGMFPTEAKASILVGHFLSYLKSGAKWQSKIACLKLVEKLIEECPADLLEMEFYDAIPILTDMATDFKPELAKDGMKVLKQFVKILDNLDLSPRYDIIVETLANPQKVPDCIKTLASVTFVAEVTEPALALVVPIIDKSLKMSSSSQEQLRQTVKITENLTRLVNNKREIEKYIPVLLPGLKQVIQTASLPEVRDMANDALSVVEEAEKEHSDGKFHGRLHKTSAAQYIQQFKNRLNSDVLFSYLTGIVVSDANVNDWNSLSEYFTMALSSYYKDDEEEKNKEVADMVLTLKTLFTPDAVKDDEEDGIVIVNAAFSLAYGARMLLNKTNLRLVKGHRYGLCGRNGVGKSTLMRAINNGQLEGFPDQSVLKTCFVEHKLQGEEGDLNLVDFIASDPDLSAATKEEIADALESVGFNEERRAQKVGSLSGGWKMKLELARAMLLKADILLLDEPTNHLDVSNVKWLENYLLEHTDITSLIVSHDSGFLDAVCTDIIHYENKKLVYYKGNLSDFVDIKPEAKAYYTLTDSNVKMHFPPPGILTGVKSNTRSVARMSNVTFAYPGVEKPSLKNVSCNISLSSRVAVLGPNGAGKSTLIKLLTAELVPQEGTVEKHPNLRIGYIAQHALQHVNEHKEKTPNQYLQWRYRFGDDREVLLKESRKIGSEDERKMMEQQIDVGDGRGPRFIEAVVGRQKLKKSFQYEVKWKNWLPKCNSWVPKDVLVEHGFERLVQKFDDHEASREGLGYRELTPKVIREHFEAIGLDGEIADHTALGSLSGGQLVKVVIAGAMWNNPHVLVLDEPTNYLDRDSLGALAMAIREWKGAVVMISHNNEFVGALCPEQWIVENGELVTKGVAEIDQTRFEDGGNSEQTKLATELAKKSVAKADDDDSPANIKVKQRKKRLTRNEKKLQEERRRLRYIEWLNSPKGTPKPVNTDDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDW8</accession>
    <name>A0A1E3NDW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial phosphate carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_18247</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015440.1">
        <property type="nucleotide sequence ID" value="XM_019161922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDW8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDW8"/>
    <dbReference type="GeneID" id="30178609"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000046">
        <property type="entry name" value="Phosphate carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="12"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="104"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="194"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="31725" checksum="2ADD1053EC94681E" modified="2017-01-18" version="1">MPDEHIPLYTPAYFRTCAIGGFLACAPTHSFVLPLDLVKCRLQVDKNLYTSNYAGIKQIIKTEGLHKIYTGFAPTFVGYGLQGAFKYGGYEYFKKQYSDLIGGSNVFVYLAASASAEFIADLFLCPFESMKVKYQTTLPPKAITNLTKNLYSGLVPLWFRQIPYTMVKFTTYEKIVEMIYARLSKPKEEYSKVQQTGVSFLGGYIAGIFCATASHPADVLVSKINNDTRPGESIGQAVSRIYKQIGFVGLWNGLPVRILMIGTLTGCQWLIYDSFKVYVGLPTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEC9</accession>
    <name>A0A1E3NEC9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015614.1">
        <property type="nucleotide sequence ID" value="XM_019164853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEC9"/>
    <dbReference type="GeneID" id="30181540"/>
    <dbReference type="OrthoDB" id="3998229at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="279" mass="30586" checksum="2AF46DE61E68550E" modified="2017-01-18" version="1">MSKYDIPYSMKPTKKGKKKQSKYSSYIEENDTFVNRLFSTPARKLIAYVTFFSIFAVIFFTALRNSKHPEGLDYELEVGFPDSKEQNLIDQTIVDVNDNTQISDANAIDVEVDEIDVLDSEDGELQLADAVETEKDMANTKPKANAQGKGVGKAPAKAASKKAANGKANGKANGQAKAQAKQGGAKGSVEDEANEAWVVKGGEAKQNSGMFGDDHFSEELKGMMSSGNNKLEKEKGKTKKGKQSRQKQISDEELIEKMDKLEAEKAKGRKKNAFKGENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NED0</accession>
    <name>A0A1E3NED0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74727</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015608.1">
        <property type="nucleotide sequence ID" value="XM_019164850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NED0"/>
    <dbReference type="STRING" id="763406.A0A1E3NED0"/>
    <dbReference type="GeneID" id="30181537"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF19">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="7">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="196" mass="21797" checksum="172A2F7024ACA5EB" modified="2017-01-18" version="1">MSIQMTLIFALLTVEFGLVSVLLLPLPPKVQSLVLRKYDEAISNSNFAIVLAFTDALVGVMFVDAFKNGFKLLERGDEIIEFSKNVWDARAKKFYSQRNLYILGAILALQACVWFMSMLLKSTVKNKALLGGLSNEGKTPSSSSDAKTPETEALEKELAKAQLDVDTLNKQYDSLFAEYQKKNATPAESDSTRKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEU1</accession>
    <name>A0A1E3NEU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015764.1">
        <property type="nucleotide sequence ID" value="XM_019164749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEU1"/>
    <dbReference type="STRING" id="763406.A0A1E3NEU1"/>
    <dbReference type="GeneID" id="30181436"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="401"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="436" mass="48531" checksum="1D9A3EAE7540A6C4" modified="2017-01-18" version="1">MNDTKGSSGIKQKQTHKLHITTEDDMHASGGGSGRNNTPDGVDGTKRPNSSLNMAASTVHLSKSGGGANGSNMSSNDKERLIDEKLLSSRDQEKREALAKQRENYLNSASTSGLEGDILLQPGARNAGRLRRFRLFLKNIFSDIDSHTEHLFYVIAAEMFFIFLTYTHYSYLDNKNRFLTPIVMGSETSLLGQTLTQLFDWTRARTALRNRTVHKTPSDEEMNLTSATNGTSNSMSTGMREMHSRNSSSNTIPVSVSPHLAPVSGNSAVSGQPLEQMHIHNPLPRTMLAADKEASLRDHLKFLIWGGVNGLLCSYWIELLLTVFRDRELVCVLVDQTAGTVIFQTLYSLYICLWDGEVEVSGAAAIADTSDSSRRTVELTWENFTNHYAGMLWKYMKLSYLVWPVISLLCFTVLSDEWIFPVNCFFATLFGVTLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEY4</accession>
    <name>A0A1E3NEY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PQ-loop repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74423</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015828.1">
        <property type="nucleotide sequence ID" value="XM_019164765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEY4"/>
    <dbReference type="GeneID" id="30181452"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="245"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35041" checksum="6D04F934A9873EAA" modified="2017-01-18" version="1">MVSATANVLATIGTVCWCVQLIPQIYHNYRRKNTEGFPEVMVIFWCLCAPFFAVFVVVEDASIPIMLQPHLFGFFCTVVYVQVMYYPPISRPKKQIILRTGIFVLFQICLEVGCIIPLRKVYQRGTTWPTLIFGIIASILIAFGLIPPYFELWKRDGRVVGINFLFLLVDLSGAVFSLASLAVDPADLDIMGLVLYTICATLEVGIFISQFVWLVRFRLFKKTKTNDLEQISRSATIENDVEFLGKDDKDESNDTDSCFSNNESASELAAEITRNGADESPAKQEKLKGTEQYLPLEQDLPDLSPYQTLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF69</accession>
    <name>A0A1E3NF69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015885.1">
        <property type="nucleotide sequence ID" value="XM_019160060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF69"/>
    <dbReference type="GeneID" id="30176747"/>
    <dbReference type="OrthoDB" id="4067208at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="224" mass="24395" checksum="BBAA4E55EFA6BA05" modified="2017-01-18" version="1">MATEVEFANKFVQLLEVTTPSADAQQAFADSFNLNSISRLPGNFTFPPLPRPFGATRQNALTDSNECKDAGETEEVIATVEFTFKSLKQPKFNLKLALDIKPSTNIYMVKSSLAQLLEGSPETAIAVQPADIKLMIRTKTLQDSENVSQVLESAGSTGSLSLNVLISQFKAKTQETKEKSPDVSIPGHAAITEASWSQISDILLKDLKDQAKVDQTIKGFKNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFJ2</accession>
    <name>A0A1E3NFJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_35427</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 12"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016028.1">
        <property type="nucleotide sequence ID" value="XM_019162943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFJ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFJ2"/>
    <dbReference type="GeneID" id="30179630"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051063">
        <property type="term" value="F:CDP reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046704">
        <property type="term" value="P:CDP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006240">
        <property type="term" value="P:dCDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000001">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="863" mass="97139" checksum="2975ADADDD9D3CF5" modified="2017-01-18" version="1">MNVVKRDGRVVPVQFDKISSRLEKLAYGLDTKHVHPSAITQRIISGVYDGVTTVELDNLAAETAAYMTTIHPDYATLAARVAISNLQKQTTKKFSEVVEQLYRYINPKNNKHSPMISTKTYEVIQKNAHVLDNAIHYDRDFNYNYFGFKTLERSYLLRLDGKIAERPQHLIMRVAVGIHGDDIDGAIETYNLMSKRYFTHASPTLFNAGTPRNQMSSCFLVAMQDDSIEGIYDTLKQCALISKTAGGIGLHIHNIRATGSYIAGTNGTSNGIIPMMRVFNNTARYVDQGGNKRPGAFALYLEPWHSDIFDFIDVRKNHGKEEIRARDLFPALWIPDLFMKRVEQNQEWTLFSPSEVPGLSDVYGDEFEELYTKYERENRGRKTIKAQKLWYSILEAQTETGTPFMLYKDACNTKSNQQNLGMIKSSNLCCEIVEYSSNDEVAVCNLASVALPAFVESDEHTSWYNFEKLHEIAKVVAKNLNKIIDVNYYPVPEARRSNMRNRPIAVGVQGLADAFMLLRLPFDSPEAKELNIQIFETIYHAALEASVELAIKEGVYETYEGSPASQGVLQFDLWGKKPTDLWDWDSLKEKIRAHGLRNSLLVAPMPTASTSQILGYNECFEPYTSNIYSRRVLAGEFQIVNPYMLRDLVEMGLWNDAMKNHIISDNGSIQNLPNIPQEIKDLYKTVWEIPQKAIIDMAADRGAYIDQSQSMNIHLKNPTMGKLTSMHFYGWKKGLKTGMYYLRTQAAAAAIQFTVDQNSAQSAGSTEAILSKINIKKYVPRKLSKVQDVEAVVPAKPASMREEFNKLKLDDEVPPIPVTVSSPATTASTTDSSNEEIAEVDIFNSKVIACAIDNPEACTMCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFX6</accession>
    <name>A0A1E3NFX6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_73818</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016135.1">
        <property type="nucleotide sequence ID" value="XM_019164598.1"/>
    </dbReference>
    <dbReference type="GeneID" id="30181285"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF2">
        <property type="entry name" value="AFG1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="918"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="962"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="255"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="351"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="469"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="568"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="719"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="760"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1618"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="483"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="596"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="760"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="786"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="821"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1619"/>
            <end position="1628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1678" mass="184888" checksum="5909E40C071DF6F4" modified="2017-01-18" version="1">MNILTPQRLQRLATWEHLGDSWFLVATATLTVCNRPQEIPRLYHYAMHTAAAAASPAPQPPDLDAILAKFADIRATGADPAFDPYAAAAAPARRRRLVLTEKLRESILKTAALSGLPRAINALTLLRDCTPASLRQQCAAGGTKRPPVASWADYVAEQQRGKAYWDRVYTKISTRVAGQLESAYPDLWTYTLQHVYAPLLSYTDVLSAEETALVVVASLVPQDVNPQLKGHLRGAVNGGVASSVRVSAVPMSDLQGLNINNSNSNSSSSSSSSNKDRAIKDSSSDLGSTFIHGNSNMDPSSSNDDTPMALLEQLVYIDNFFQQSDSPNVQGSDNFDDQLQRELAAFANDTFIFPDEDKPKQASSNGEGSSGNKAGAKNPPAGNGGNSDNLLSFAQASFEQISPADMSSVSSPIPPTHYQNLSQRYNNSKQKFTYQESMSQHMTTIPSTIHELLGSGNAAASTSPANLSAASNAASANPPPTTINPAASSSRSNNNSNASDLFDNPRARHHATNSTSTSSTNPLDAGEARKVQVPQGAQNTLAAAGLSQTQIDALASLVAFHKSDSLGKAQASSGASGQFPPFNLQSSQPSTPQNFQLLQELSNQQRQQQPQQPQQPQQAPSNNSNNVNNLLVGLLTQSLVNNVGSPQQQPQQQQQQPQNNLASSLLSLLAGNAFDNGNQGNVLNLLLQLQQQQQQQQQQQQALNLQSILSNQIQGGGLSTLSNTPNSTQLTGSAGSPTLAAGSNNKKQTLTFKNSLLDMSKSTSEPVVRKSTSSFNSIPANTKNTSSISHAPGSSSSSLQAAASAIAAERKEGSSKAAEPSSGNSSATSSHAAGSGDSARTAKGTASVRFRQKKKLKEQEMEKNLQEAKEVADALQARIQQLEMENRLLRDLVVEKSHQRDSEEVERLRKKARLQVKAWFCTVVSAFGVVILSVIAGLFYANHETMMGSITSPSDGKGVAKTILGAIVLYLAFLAFCGCQILTKSGKMLRAANRRCSFKFASVAAQQLRLVRWQTTTSGVLGTNRFTQTPRALNSQNIYGSANESSTIKSTALSVTDPYIVYKHLVNSKEIEPDPHQLASIKKLRALSESLQSYKPQLTSVKINRLVRELEIRYNQQKQDGKGVLFGYTVGWFRERENTKLRTELVKVLNDYEELTSPEMKDIPKGLLINGEVGCGKTMLMDIFADSLPIQGKWRIHWGVFIQWVMKEIESISSRKRDRFMQANHPLLNYENEFVLFEVAARMIEKCHVLILDEFMLPDIASAKIVNVLFVYYFKLGGVLVASSNRLPEELYSGSINKSTMGSFEQILRARCDVWDMQSEIDYRTNLKEDDIADEKWMVLKSDENHHEVWKNLVEKFIDLQNCETNAKGFTSYGRDIIIPKLSDGVAYFEFDDVVRDSAYGPGDFISIATKYPTIIIDNVPILSIKMKNHARRLITFIDAIYDSRCKLVIKLETDPVHLFFPDIRDNTPTRFKCTPLEDGPNPVCLSEALKYKNDSVTTTEDVQNIEMFAKTEMDLSNPYRPNFATYEDDNKAYDIDKGEEGMKKNFTDMKKFTGEDEMFAYKRAVSRINEMTHSIRWRHTKWIPLHDSIKPWESVNGAAEKEVENQQQESVLIQPKNSEPDKEKEVAQKSGAVSQRTDAPEFKDYNFWSMGNWKNKKDKLQDEIARRWIQGIGNSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG10</accession>
    <name>A0A1E3NG10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17561</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016172.1">
        <property type="nucleotide sequence ID" value="XM_019161646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG10"/>
    <dbReference type="STRING" id="763406.A0A1E3NG10"/>
    <dbReference type="GeneID" id="30178333"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF2">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT SRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="261" mass="30981" checksum="AD5E7EA584512255" modified="2017-01-18" version="1">MDGNRRFAKNHSLPLKEGHRYGAEALMRVLECCFRVGVKNVTTYAFSIENFNRKAEEVDTIFSLLKYKLALLSGENQMCDLHEVRVKIIGNRSYLPDDVLQDIELVEEKTKHHTKHTLYVAFPYTSRDDMWYAVDQLVDKVTEGEISVDEIDETLYENNFYYEDTSEKVDILVRTSGHTRLSDYMLWQCHQDSVIEYPNTLWPNYRFYNTWWTIFRWSYYKTLMIQDAEVMQLKKVSRAEVNRRYKDVPKIHPPFASVNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG13</accession>
    <name>A0A1E3NG13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_73858</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016184.1">
        <property type="nucleotide sequence ID" value="XM_019164609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG13"/>
    <dbReference type="STRING" id="763406.A0A1E3NG13"/>
    <dbReference type="GeneID" id="30181296"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000001">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000005">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="14">
        <location>
            <begin position="10"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="327" mass="35950" checksum="569DAAF3AF83BE86" modified="2017-01-18" version="1">MSNKEIKNNIKVLAANSHPELANLICKRLNVKQSDIEAIQFVNKESTVIVRESLRDEDVYIIQTGCGGFEKLNDYIMELLLMVHACTQASARSITAVIPNFPYARQDRKDKSRVPISAKLIANLLTTSGCDHVITMDLHASQIQGFFSIPVDNLYAEPNVLKWIKDNYKDTNNVMMVSPDAGGAKRVASLADKLDTQFALIHKERAKANEVSRMVLVGDVKGKSCILVDDMADTCGTLCKASDILLAEGAKEVIAIVTHGIFSGNAIERLNGSSLKKIVCTNSMPLSEHVKQCPILEIVDISPILAEAIRRLHNGESISYLFNNVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG23</accession>
    <name>A0A1E3NG23_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016208.1">
        <property type="nucleotide sequence ID" value="XM_019161659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG23"/>
    <dbReference type="GeneID" id="30178346"/>
    <dbReference type="OrthoDB" id="3994897at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="363" mass="40209" checksum="C891AA67900FBC34" modified="2017-01-18" version="1">MQGTLPYALAAHGHSGVAGIGDALIHDATDNSFNKKSVDRRSRVTGQNREDVGISNVSMELAGSGVSGNSEWVLFSPYDEGEEEEEEEYDAAETGSILSTRLSSFQSPIKNRRSSSFETTGYVISDFGYSLKSGYVTDEGKNSVTDNGVEDADEGYDDEDYEDDEEEDDEDDEDGDDDEDSLAGDVRSGIKDMQRSRLDEIESKKHDELVSKINTWKHSVKTVNGVTPKVDGRLTMGNEKKREVKAEKRFTSVKEHKARESEIRVFKSAASRLCSSLKKERLVEHEGIFMNNPELESCVPGYWKRMMLDNLIQYNAEPGAASVGYNILGDDVKGGNRNFWEPEENISVQSVSTGWEGGSILGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG33</accession>
    <name>A0A1E3NG33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH TFE/IIEalpha-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17589</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016204.1">
        <property type="nucleotide sequence ID" value="XM_019161657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG33"/>
    <dbReference type="STRING" id="763406.A0A1E3NG33"/>
    <dbReference type="GeneID" id="30178344"/>
    <dbReference type="OrthoDB" id="361102at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="233"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="318"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="473"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="550"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="566" mass="63410" checksum="61752928A4F0EECB" modified="2017-01-18" version="1">MSAVSKAFQVNDDAVKHLLRQVVYMFYPHQSIIIMEAILFHNVLFEDDLIKLCCMHKKVFRSFCNKLMEDKLIVAHTQKEETQPYKLFTRTYYYVHSIEAIDSIKWKIHTIVKHVKEEIGNFSEPQGYICPTCHHKYTLLDAASLLTDDRLNFECSICGDYLIDDDISQEQKKGQEKLERLMSLVEPIIKYLKIVDDLKIEDNNFDTTLIKYVPAFSDSLALYSVSTRTSARRKKLENNNTNLSDASKRSQATIHVSITADDEDLKRERQKREDRNKKLRQNALPSWHQESTVGKAALGRLDLEEEEDEDADLIQQQDQENPANPAQPDNDDGTTTTSATTANTIQDNSTLINSTDGTNVAVKGENGQQAPDSSTSPVKTEQPAISDTDSTMQLPVKAEQTAEPSNTAPMASVQMKEESAAGSSTKPSTASTFMSTTSDKTAVPASATEGTTAEELDALSAYYSQLRQRQAAEEEEEEDEDEEEEEEDNADGMDIDAIDEEDVDNMDAFNDEAENGTAEAVKPTQTTDTTDVKPTADDTKQADDSINKDDLDEEDFDLDMFASDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGG5</accession>
    <name>A0A1E3NGG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">holo-[acyl-carrier-protein] synthase</fullName>
            <ecNumber evidence="1">2.7.8.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17731</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.8.7" evidence="1"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016353.1">
        <property type="nucleotide sequence ID" value="XM_019161715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGG5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGG5"/>
    <dbReference type="GeneID" id="30178402"/>
    <dbReference type="OrthoDB" id="26719at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050559">
        <property type="entry name" value="P-Pant_transferase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215:SF10">
        <property type="entry name" value="L-AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE-PHOSPHOPANTETHEINYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215">
        <property type="entry name" value="PHOSPHOPANTETHEINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="3">
        <location>
            <begin position="140"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="297" mass="34166" checksum="A5EA2D909E691A0C" modified="2017-01-18" version="1">MTLSLPQIVAKENRDNTLFIFYANLLDESVDINESKVVQLLRDDFKFELLLRNLSLPHQLKVRNIKSELDRDVKIINMLILKYILKRYEAGNVHSNEWEESKNEYGKPSLKHRNYQYNISDEIGMVCLAIGFNEKLNSEVGIDLANPDDIQRFGMSDLRSFYENDFRSIFGAKEILELDRSFGDLTYDEQLQRLTQIWALKESFCKYLGVGITAGMENFEFPKPKKPSVIEGGVEGSKDLFQVVARDIEMNFTGVVDVRNGCFQLPQSKLICSVFGDYKKACLVKVDVGNIIKEFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGH0</accession>
    <name>A0A1E3NGH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015781.1">
        <property type="nucleotide sequence ID" value="XM_019164753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGH0"/>
    <dbReference type="GeneID" id="30181440"/>
    <dbReference type="OrthoDB" id="4029988at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035283">
        <property type="entry name" value="Fmp23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17315">
        <property type="entry name" value="FMP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="182" mass="20703" checksum="05D89F993759198F" modified="2017-01-18" version="1">MSQSRPAKSSHSKESEDLVTKAHLEQLKPENSAAFAFKYFDELSSHENPILTAEYKQLPDDSQFIDKHYDELVKYRDLICGVGGQFGEISGVPELFLRLSKVHPKHVVASTDGPEQTETQAIQGPAKEMKDSFLKMQVDQFDQFVIDLMNALRLNGGHLFVLDLLIYNNQVFDHCERTHHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGK7</accession>
    <name>A0A1E3NGK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17753</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Degrades free oligosaccharides in the vacuole.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016380.1">
        <property type="nucleotide sequence ID" value="XM_019161724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGK7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGK7"/>
    <dbReference type="GeneID" id="30178411"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034270">
        <property type="term" value="C:Cvt complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019309">
        <property type="term" value="P:mannose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000004">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000002">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000001">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054723">
        <property type="entry name" value="Ams1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22907">
        <property type="entry name" value="Ams1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="9">
        <location>
            <begin position="587"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1139" mass="127851" checksum="9A3A657DB4067D20" modified="2017-01-18" version="1">MATAHSHSKGGCTAAAAATSQYPQLNDHPTFKTVDSIYENRLRQFIDKGQYRDLNLPKFYNRERLTLSNVSSPSETSDSGIWLKKWAAPGLSKPLFKDIIPSKLGEFKTCPAGEGITMSPKWSSHWFEVRVRISKAWYDDISTGKGKENSEVLTFLFDAGCEALVFGTDGCPLQGLTGNGERNECELPLEWFSKAKSGDPEKLEAVFYIECGCNSIFGGGGDSYGVTAVEIIQYNYQAKALYFDFLVLSDAAREDGPPQKHKAREVCNRIMDVFDPNDITTVVACREIAKEFLGNNVDSAEVFKEKKMNKFNAVYAIGNCHIDTAWLWDFATSKTKIARSWSTQLRLIEKYPEYVFVASAAQHFKWLVQYYPDLYKKVRQAASEGRFIPLGGSWVENDTNLPSGEGLVRQFLLGQRYFENLFGAKSDIFWLPDSFGYSSQIPQICRLCEIPKFLTQKLSWNNINVFPENSFNWVGIDLSQVLVHMPPDNTYTADANFGDVKRSVINHKNLYDDQKGMLLYGKGDGGGGPTPDMVEKLRRCRGYANTAGGDFSQVELGCTVSDFYNDLSKDTDNGKKLPSWRGELYLEYHRGTYTTQAKVKNYMRTVELLMHNVELFSTIASVLKKKFKYPLAKIEALWENICLCQFHDVLPGSCINKVYGEDVWPMLADVVSKETKLLEAALAQLGLSTTKDDTHKHLVKLSSLPWKKSSIEIVDNLPNYLLSIAQKVDNNKNLVVMTGCTVMKPLASKIKYPSSIIKNDSGLFVMENSKLKVVLDSDGVILKLYDIVNKREVIDGTTMKGGNQYVMFSDTPLNFPAWDTELYSLNKFKYVSKSTKCSIKYNGPLVSALEVEHELSSSSKITTIISLDGLNDLSETSSVKFKCNVSWNENYQFLKVQFPVNISSETASYETQFGITKRPTHFNTSWDVAKFECCMHKFVDFSDYNYGVSIVNNNKYGGSVHGNIMTLSLLRAPKYPDEKADIGDHSFEYSIVPHSGPLGATTVRAGWELNERMPSELYVATDKKDSLKTVEDFLTITGDEGIILSNVKRGEDDADVDYDGGLMDNLPKKYVGSQTVVVRVYESLGGISKATIKLNAPVTKIFKTNLLEEEKEEYEISSSGEFTFKLRAFEIATFKVIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGM7</accession>
    <name>A0A1E3NGM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_19643</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016400.1">
        <property type="nucleotide sequence ID" value="XM_019162132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGM7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGM7"/>
    <dbReference type="GeneID" id="30178819"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133349" description="Calcineurin-like phosphoesterase domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="51"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="372" mass="43531" checksum="118E10E2AD9DE1E0" modified="2017-01-18" version="1" fragment="single">IRWKQLATLFLLMWSVIHYFERVKPYYVFQSCLWKEWESWPHESDPHHSIIIGDPQIVDNYSYPSRSWLELTITKIFSDNYLHRNHNIYSKVLDPDSIIFVGDLFDGGREWNDKVWLKEYVRFNKVFNPIEGVRQLRQIPGNHDVGFGNGIDFGKYSRFKAYFGNADEVVVLGNHSIVLMDTVSISCIDNNKISQASSKFLRSFEDPSNTYKELPRIVISHVPLYRFTELQECGPLRESKKAFPVSRGNQYQTVLEYELSQKIVNWIRPIMLFSGDDHDYCHIRHPLDKRVKYTDEITVKSSAMTGGVKKPAIQLLSLWNPNNKQDDTWIVSNEETRKVDAGTAETYLCYLPSPYQPLVHYGITLAFSIWWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGQ6</accession>
    <name>A0A1E3NGQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Manganese/iron superoxide dismutase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_36034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016417.1">
        <property type="nucleotide sequence ID" value="XM_019162980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGQ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGQ6"/>
    <dbReference type="GeneID" id="30179667"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="202"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="256" mass="29730" checksum="8640181DA4B32FBC" modified="2017-01-18" version="1">MLFGQVIAKRGIHVAPKLALESSFRQEGVRGLMSAEQFKTAWVDYQDFLTKNLSMKTADTEYETRSPMAIAMSTSKKPHQAPIFHYASQAHNNHLFFQQLKHNGARTPVQESDIKPQLLRSIKNTFQSLENFRNEFLLAADIMSGNGWVFLVEDQNKELKIVACNNDGTPYFYGRNQLYDLNTVFEYQNYKKLMKYKQDILDNVKDYSLPLLCVNVWEHAYIVDYGVTGKAEYLEKVWDNIDWNVVNSRLFSNIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGS7</accession>
    <name>A0A1E3NGS7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">QRI7</name>
        <name evidence="9" type="ORF">PICMEDRAFT_35494</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016432.1">
        <property type="nucleotide sequence ID" value="XM_019162949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGS7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGS7"/>
    <dbReference type="GeneID" id="30179636"/>
    <dbReference type="OrthoDB" id="10259622at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072670">
        <property type="term" value="P:mitochondrial tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="8">
        <location>
            <begin position="72"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="404" mass="45287" checksum="B8FB8C9D1B69EA10" modified="2017-01-18" version="1">MCALLYARCAGRRLVQNSQWLQRLCVKNQIKGYRVLAIETSCDDACVTLMDRYSPDEAPKVVDERKVTLDSASAGGVVPTDAANHNRKNISKLVNQMIIDNKWGSAETKPDLVCATRGPGMVGSLAGGYQLACGLSIAWQVPLVGVNHMLGHLMVVRMESNGKEPQYPFLSLLVSGGHTMCVLSHSLTHHEILVDTVDIAAGDALDKCGRELGISGNMIGREVEKLLDQHREEWGKPVEIMVKEPMLNQWDRKDSLQYSFASFNSQVKNTLKKFYSSEIPQDETIKARLLFRFQQTIFQHMISKIKLAIKIHKLDVKNFICSGGVSSNLYLRKMLKDQLGPAGIENFYFPEPRLCTDNATMIGWAGIELYEQGYTTDIGTTVIRKWPITKLLELEGWERKPNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH58</accession>
    <name>A0A1E3NH58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17917</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016562.1">
        <property type="nucleotide sequence ID" value="XM_019161805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH58"/>
    <dbReference type="STRING" id="763406.A0A1E3NH58"/>
    <dbReference type="GeneID" id="30178492"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 13" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="14">
        <location>
            <begin position="31"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="121" mass="13531" checksum="4C45FF513D3B3ACB" modified="2017-01-18" version="1">MFRQTARLALRQSGALGRLAGSGYRFQSTLSANEVLKRSVAVLKTFEIKTPEQPITMTTQFGKDLGMDSLDYNDALVALEEEFDVVFDDKVANEITTVGEAVDYIAKNHMPEEDILDKEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH69</accession>
    <name>A0A1E3NH69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015806.1">
        <property type="nucleotide sequence ID" value="XM_019164759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH69"/>
    <dbReference type="STRING" id="763406.A0A1E3NH69"/>
    <dbReference type="GeneID" id="30181446"/>
    <dbReference type="OrthoDB" id="429467at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="154" mass="16881" checksum="AC8FFF6F71EB489E" modified="2017-01-18" version="1">MTDQTDGNKDFTKSQIQKYRMLFNLIDQDGDEKISVKDLKVAASDVNLKLDDSVIKDMLKSAGGKSVDFNSFLKIAGAQFGGFSEESELRDALTTFDGKDGIDSAVLKSSLGELAESDADKKAVAGVVDEFTKENKITGYKKFDSEKFIESVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHA9</accession>
    <name>A0A1E3NHA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_73055</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016648.1">
        <property type="nucleotide sequence ID" value="XM_019164368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHA9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHA9"/>
    <dbReference type="GeneID" id="30181055"/>
    <dbReference type="OrthoDB" id="204405at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004538">
        <property type="entry name" value="PRK05888.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004539">
        <property type="entry name" value="PRK05888.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF20">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="148"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="217" mass="24741" checksum="DC531B828A7D17DE" modified="2017-01-18" version="1">MFARPALSRGLVKSYTRSLSATAMLKSASATAGSAISTRPEGFRIPRPRTWEESKEGALAKATKWFLLAEMFRGMYIVLEMYFRAPYTIYYPFEKGAISPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEERADGSRRTYKYDIDMTKCIYCGYCQESCPVDAIVETPNAEYATETREELLYNKEKLLANGDKWEQEYQYAANADAPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHE1</accession>
    <name>A0A1E3NHE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016678.1">
        <property type="nucleotide sequence ID" value="XM_019164376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHE1"/>
    <dbReference type="GeneID" id="30181063"/>
    <dbReference type="OrthoDB" id="3996053at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="407"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="301"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="442"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="480" mass="55619" checksum="C3C6A42DD2901DB4" modified="2017-01-18" version="1">MSYIPALKTPIGQPRRSFRTDSQGIASSENGTRADRFAEISKSRPAPSQVRFKSVLPSSKNSISLNQNERERRKHEALEFLRKETRASHSTNHSIPRKYTSRQNNPDSLKTYQLLGERPIDRIPGSSSRSAIYDDQTVKNITRESLRRNPLGYTSQISDRRSRVSKPNNSFASKNNGLLSSLSNFGSKVFRSILYSEQDTNPNEPSSQPSFSSLGSFRSDLHKEQDDILRSEIKQKELDLQLEERRRYLDELNRQIVRTEKTLASDSPKTIMDQSKSIDQQIGKLDERLQNILEEVNSTSNGKLLKELESIKGELTTLRRKQESNNMKFESRFEDMKLENQLNRQKFDQLFEELEEKRKELDLEKDTLIKILQRNGTEHTNTKFDKRKNYTSEANIDTTARKRVNSYTKGIFSDNENGENDDDDDDDDDDNDDEAGSSDIMKYIKENRRRNTDYTPVRVRNRIRQITNSLKKIEKAAKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHN5</accession>
    <name>A0A1E3NHN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Respiratory growth induced protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the control of energetic metabolism and significantly contribute to cell fitness, especially under respiratory growth conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RGI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016773.1">
        <property type="nucleotide sequence ID" value="XM_019161408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHN5"/>
    <dbReference type="GeneID" id="30178095"/>
    <dbReference type="OrthoDB" id="4082176at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006112">
        <property type="term" value="P:energy reserve metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.40">
        <property type="entry name" value="Respiratory growth induced protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022554">
        <property type="entry name" value="RGI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038235">
        <property type="entry name" value="RGI1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10843">
        <property type="entry name" value="RGI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="180" mass="21458" checksum="2546B7E74E0CF918" modified="2017-01-18" version="1">MKKDKSSKIELEPRIVDHLSKEPAVRNSREVKFDDLKKFEDYIKNESWDDEYDQMKVHLEYIPPFIVNEIHGEEEHIKPQMNALNKKFRRHLHQHLTKHLLPEINKMSGIGYQFKEIDEGLVPDFNGVDSTYRWHYEDLGNHGFDEDEYKDRHHWDATLDVACDSNGPFVNAHFVCQPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHY7</accession>
    <name>A0A1E3NHY7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_16997</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016819.1">
        <property type="nucleotide sequence ID" value="XM_019161421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHY7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHY7"/>
    <dbReference type="GeneID" id="30178108"/>
    <dbReference type="OrthoDB" id="5584247at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052246">
        <property type="entry name" value="Cell_Polariz_PKAAnc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155:SF1">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155">
        <property type="entry name" value="A-KINASE ANCHOR PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="312"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="503" mass="57268" checksum="B24ABC9C22C72194" modified="2017-01-18" version="1">MPGADAEVSLPAETSDHHPIQSGSGNNNANVIKSKLADVSDLQDYYDALDELEKQNGKLQTFNTMPETDTRDRWPTLFEILNKKTQSPLDLWSFYVFMRDEAHSIDYIDFWIDTVQHINLCKVYVKGLKDSLLVSARARQSDIVSNALNVNNKSLLIGEIEPPQTRYSVKRSDSVKHDAATNRASQNSSKNSGSSSMLLDLLMKHDLFEGEDPHRLSTFLRGEHSVRTSDPVVNARIDEIKRKSQNFPSSTSINQADITEGSKSNHSSSNFRISRINPEMVETLIQDDFDTKHRSYDKAHLISRVSLRKSTRNILNTYLSDDAEKRIDLPTEILDKVRFELETQGRDDPEVFDEAREYVFKAMEYDAYPNFLRNHALKNVTRKSAAIRIMLSMISGLAAFWTGYTLIFMNYEPKPTRAVVVVPFFFMSYFFFTAFYRVDPILCYAGYCESNSTPGGVLPIREPYVKKLLRKRSWFVLTVLCAVAAAFSVIFALVPGRRLTHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHZ3</accession>
    <name>A0A1E3NHZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016847.1">
        <property type="nucleotide sequence ID" value="XM_019164423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHZ3"/>
    <dbReference type="GeneID" id="30181110"/>
    <dbReference type="OrthoDB" id="1057137at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047574">
        <property type="entry name" value="AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019181">
        <property type="entry name" value="LSM12_ABD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09793">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52001">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AD" evidence="1">
        <location>
            <begin position="83"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="201" mass="22524" checksum="01FE3EEDE49D63A3" modified="2017-01-18" version="1">MLLLDNVVGLKIHSYSPHTLLTVIATPSPSSDNASGDSKYTKNATFIAIKVQYVKNIQALEKDKPRGKAHPNEKFGLKINAPTFIPTETLGSNIRRGSKEMTKQEIEFKLRRMTKGKKLSDEGRKVLRNLAAIFPIDTLSVDDSNSITFRDSDIKIMKPYKSSDVKVIGCDGGPNEQQQLAYIRKVVDREWEKMEQTQKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI38</accession>
    <name>A0A1E3NI38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-specific glutamate dehydrogenase</fullName>
            <ecNumber evidence="4">1.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">NAD(+)-dependent glutamate dehydrogenase which degrades glutamate to ammonia and alpha-ketoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.2" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016897.1">
        <property type="nucleotide sequence ID" value="XM_019164436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI38"/>
    <dbReference type="STRING" id="763406.A0A1E3NI38"/>
    <dbReference type="GeneID" id="30181123"/>
    <dbReference type="OrthoDB" id="184415at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056365">
        <property type="entry name" value="NAD-GDH_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016210">
        <property type="entry name" value="NAD-GDH_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055480">
        <property type="entry name" value="NAD-GDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF24">
        <property type="entry name" value="NAD-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23147">
        <property type="entry name" value="GDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23152">
        <property type="entry name" value="GDH_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000184">
        <property type="entry name" value="GDH_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="654"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1026" mass="115075" checksum="1609B60F197F4FA6" modified="2017-01-18" version="1">MAISAIPNIQNLTLDNNFSQSSFENISKEDVVANGYVASVFESKHDQINKVMDQLDSTGLIPENLIESETKFFYENLGIDDTYFASESVENIASNILAIYAAKIDAAINGKNTSSIHFKKITEDKHHAVYFETGDSNSFEKEIDDEFLDAKDSYYRVESFNSVLPSEELIRCHFVYKSDFSESETLSSSSLIESISDKTFSKIATPYTKTLYSEVLTQLKDTEGPVVKHYTLSETGELRIIIGFKKGSSHRYNSALSTLANYYGATLKRKYVENFSNNYTIISMYLANSHEVETGLTTLQLTKDASLLYCIPDNKFYSKFESGIWSIQECIYAHSGVIFATHFLNRLGPEFQSLKELLLDSSNVSPKNVELVSKIKERFTSETFSESYIMEAFESNSNLIRQLYKNFVDTHYTSSSIVKTLSYQRASKIEGIQNDEQFERALAEIPNDGYSTVLSILYAFNKSILKTNFFLTSKVALSFRLDASFLPKSEYPNKPYGMFFVVGSEFRGFHIRFRDVARGGIRIVLSRSEDAYVKNMRSMFDENYNLASTQQRKNKDIPEGGSKGVILVNPGSAQVKPKECFIKYIDSLLDLLIKDPSKEKIVDLYNKPEMLYMGPDENTAGFVDWATLHAKKRGAAFGFPWKAFFTGKSPTIGGIPHDEYGMTTLSVRAFTEGVYSKLNINDWSLITKLQMGGGDGDLGSNEIKLSGDEKYVGLVDGFGCIVDEDGIDKQELLRLANNRFGNNKFDKSKLGPKGYVILVDDKDVTLPNGKIIHSGIQFRNQFHLKLQEIYPPDFIKLWTPCGGRPNSIDVTTVHSLLDPKTGKSIIPIIIEGANLFITQAAKYILEGAGAIVFKDASANKGGVTSSSLEVLAALSFDDDGFSKNMCMDPETKALPAFYQQYVKEVQSIIVKNARNEFNMLWKLKDENKKSFADLSDELSVAINKLGDELSSSKELWEDTEFRNIVLKDALPSLLLNEVGLDGILKRVPEAYLRAIFATKLASEFVYTRGIDANPAKFLEFVSSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIA7</accession>
    <name>A0A1E3NIA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_35309</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the actin-binding proteins ADF family. GMF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016967.1">
        <property type="nucleotide sequence ID" value="XM_019162933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIA7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIA7"/>
    <dbReference type="GeneID" id="30179620"/>
    <dbReference type="OrthoDB" id="3919494at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011171">
        <property type="entry name" value="GMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249:SF2">
        <property type="entry name" value="GLIA MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249">
        <property type="entry name" value="GLIAL FACTOR NATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001788">
        <property type="entry name" value="GMF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="139" mass="16003" checksum="A9EA0867FD9FC556" modified="2017-01-18" version="1">MSQSICKIPSTTKQQLRKFRLTPIKQSTTTIPTQLYKIDKEQELQLDESLDLENFDDLAEELPDNSPCYILMNYGYTKPDGRFVSPLVGIYWRPDTARGEAKMLYAGAVELFKKEVGCNMWLECSDEDEIPDLKAEIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIJ0</accession>
    <name>A0A1E3NIJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017063.1">
        <property type="nucleotide sequence ID" value="XM_019164476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIJ0"/>
    <dbReference type="GeneID" id="30181163"/>
    <dbReference type="OrthoDB" id="5361617at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50567" checksum="9C1574912463AFFB" modified="2017-01-18" version="1">MDSQPVGHYYILDPSALVYGGIGKIKEWVNSTVENHRKGNKTFRIVFYVPSYTLKELDFLRKVFNPLISANARESIKFIDSQISGVQFDGDENDYDIDIDRLSLSDNDSDFGDVSEGEFEQDEVEVSSPHVNSSEHGSVEDSQKSRGKVDKTKRSESNDYTDFESYNKMKLVRNEAKSTKKDPVRPVTFILEPERSVGPDWKIASGYRRSTPLLSDLPKPINGFSSRGQLVNDDNRNFAVGVFGNNIPGSFNVSAKSNNSHAVNATNPSSNGDGEKAVVPLRLKFLIRSCIQKQYIESKSLKEEERIIWNVICEDATTTIWLKNFGLTVKTLNDVQDDFDKFSGGLGAKVLFDPSSGKLVETTSAKFNRKTVNKKAGGAKAQRVKSKEGLNLVKSEENESTANANGITITKTKARKAKKTQKTPKLDVSANTGPAKKHVVSADTYAERGQGELWTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIS3</accession>
    <name>A0A1E3NIS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWR1-complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_21601</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017160.1">
        <property type="nucleotide sequence ID" value="XM_019162214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NIS3"/>
    <dbReference type="GeneID" id="30178901"/>
    <dbReference type="OrthoDB" id="445677at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011421">
        <property type="entry name" value="BCNT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407:SF1">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07572">
        <property type="entry name" value="BCNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51279">
        <property type="entry name" value="BCNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCNT-C" evidence="5">
        <location>
            <begin position="218"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="289" mass="31273" checksum="00582021A97DF019" modified="2017-01-18" version="1" fragment="single">NGSGDDEEDGYDEDEDEDYVADGGSKEKTGGANDGANGDVEGDEDDDEEFIDEDDRKEIAKYSSIESSEGGLIKTRRQRLEEDAKEKKARKTVHVHGSGVGKESPSAKVDINSIWAELNSTSKAGPTTAATHVVSSSPGTTLGAAGHSVDEKVKITRTYEFAGKTISEEKWVDADSEEAKAHLNSTAIPAAKSAAAQIAPEPVAASAAVPQKNLRRKRKRASLLDAVITNSSNTKLTTLEKSRLDWATYVDKNKISDELKYTNKAGFLEKQDFLSRVDSRRDSLYSKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIV8</accession>
    <name>A0A1E3NIV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Leucine carboxyl methyltransferase 1</fullName>
            <ecNumber evidence="3 8">2.1.1.233</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17305</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[phosphatase 2A protein]-C-terminal L-leucine + S-adenosyl-L-methionine = [phosphatase 2A protein]-C-terminal L-leucine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:48544"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="2.1.1.233"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.233" evidence="3 8"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017190.1">
        <property type="nucleotide sequence ID" value="XM_019161538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIV8"/>
    <dbReference type="GeneID" id="30178225"/>
    <dbReference type="OrthoDB" id="203237at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018423">
        <property type="term" value="F:protein C-terminal leucine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016651">
        <property type="entry name" value="LCMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600:SF21">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016305">
        <property type="entry name" value="LCM_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="220"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR016305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="405" mass="45003" checksum="D27515753AA25E34" modified="2017-01-18" version="1">MITGGDTDEAVRQTDFDALSSRLSCFLKGYTPEDRLLGALVPLLVHYQVAGCGSEFKRFALARRLRSQFFNVLPANIGDVAAVAQLDRAVVAAKTGRFTPLKPPLINRGTYLRTTAISRTVEQFVARHRHAGKVQVVSLGAGSDTRAFGVVAQNANVHYYELDFEQTARLKKLAILSSEQLSACVGAPALPPAELPRTAAQIAAVDPTLHTDRYHLVPCDLRGLTPERALHTVPGLERLDGAVPTLVVSECCLCYLSQDDSDNVVRFWRRSLDDGEFVVYEPLGGSDQPQTAGDAGRQPNRFGEVMVKNLICRGIEMPTLLVYGTVAAQIERFRRLLDTDTQHRCDVWCKDMKWVYDHEVDDAELERISRLEMLDELEELNLINSHYCLVVARWQPQPLALAQCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIY9</accession>
    <name>A0A1E3NIY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17324</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017212.1">
        <property type="nucleotide sequence ID" value="XM_019161545.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NIY9"/>
    <dbReference type="GeneID" id="30178232"/>
    <dbReference type="OrthoDB" id="242910at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13980">
        <property type="entry name" value="STKc_Vps15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="27"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="639"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1077"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1496" mass="170334" checksum="C70558A8ECC90101" modified="2017-01-18" version="1">MNFDFSSISPTAHTIAISAYIDFLPSLQYSKPLGTSRFLKTIQCLSPSGYVVVKLLIKPPETEIDMHVYLDRLNELKSQLHDIPNTLPFDTLIDSERATYLVRPYVRYNLYDRLSIRPFFESVEKKWIVYQLLVTLSKMHENGICHGDLKTENVLLTSWNWILLSDIGLFKPVYLPDQNQSQFSFYFDTIQRHSCYVAPERFKSQAEIDELSKNPENFKLRPEVDIFSLGCVIAELYSDGLPIFSLPQMFRYKKKEYIPNLDAIEDSNIRKLIRSMISLDPKERLSAKQYLVNFRKLLFPDFFYNFLYSYVKNLSTYSPTSTDPSSQFQICDYRIDRLYQDFDKISLYLGFKRNIIEADENNTQKRNEASLIPVELNLPGMNKHIPQNTAAIFKDESVNDCSSLILLSILCHNVRNTTHSSYRIKACDLILAFGEQLHDEAKFDRCLPYLINMLDDPSESVQISALCCMTQLLMMIDTITPINVHVFTEYIVPKLQLFLKRSYMAIKQKSLNMIASPSKFEDRSQPQTSSKSPDVIRGSYVRMVFACCLPYLAMTSKKFYELSVLLKNRVGTYRDPDIDSIIFNDRDRNEFEYAEIVQNFEHLTIQILTDSNIYVRIALMRNIRPLCSFFGKDKTNDVILSHLITYLNDKNPQIKLAFVSSIVPLSIFVGVTSLEQYILPLLVQALYGPEETIVITLLKVLTELINLGLVRKECFWDLVNLSVVLILHPNGLIRQSVLNLIIVIGKQLSMSDFYCMLYPLIRPFFQHEVTSFVWENLYIAAHTPLPRAVFNSLVLWSLSTQDSLFWQRLSIENGSKNIDMFGNAKLIFSKKRALNGSTSSSNGGRGNADDFVANFEVPLSSSDAEKIEKLKGLGLKSNELWKIASLRSYIYKVSRLSLRASKCKIGADVTRLIPRSVFIDVIHRYEANDRTEDYIISNPAENNRNLSLSSNNNGTKERGGPLILRNLQTPKPVVTTSDKMAFGESSNNFLKRENPLFEDFGDANVYDESMQLKKLTTDISYSYPGRNPFILKFLRSLVFEPDLQDYHEFGQLVDTTSIGVSEPVEGTNKTCVLVSRLIDHKASIIDLIVAPDHEYFISGDILGVLKIWESKRLEIDVTGEPCLSVNLGSPIKSICMMKDRHCFAVSKEDGSVDIFRVDFVSTLENFKLKVYPTTSITLLKHYKVGSDDQYASNLRFSLIRNKPYLFMVTPTSKIIGVDIRNMKEVYNLQNNIVHGSIQSLYVDGMQTWAVAGTNKGVIDLWDLEAGLCVKSTKFKHSSYGITKMVEVSNVLSSSKNNEKFVCFVGGTGDSDVIIWDVERAQPRIVLCSSSKKSFSTIELYSVCEVNDEIEDSILDLYINKKHVESDNGCTSVAYSSFDNSSKQGKILAAQHNRKIIEWDLSNFEDSRVVVDVRGKDGHVGDYPVYSETQVNSMLTFVHERYISAKNKDTTASNRIGNGPKVVSKAPCESISSLTYLQVPYKMVVCGDQSGAINIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJI5</accession>
    <name>A0A1E3NJI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Knr4/Smi1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_58645</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KNR4/SMI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017420.1">
        <property type="nucleotide sequence ID" value="XM_019163313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJI5"/>
    <dbReference type="STRING" id="763406.A0A1E3NJI5"/>
    <dbReference type="GeneID" id="30180000"/>
    <dbReference type="OrthoDB" id="2305498at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009203">
        <property type="entry name" value="Knr4/Smi1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051873">
        <property type="entry name" value="KNR4/SMI1_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432:SF1">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017023">
        <property type="entry name" value="KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Knr4/Smi1-like" evidence="3">
        <location>
            <begin position="135"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="397" mass="45167" checksum="C929ED8DED16AA58" modified="2017-01-18" version="1">MGFLGKKWNELVYTLTSNDRYAGYHSSKAVRSLGTESDDSDAEEAKDTGGVASGYSDQLDESGYSADEKQQAQLVLDAWHIIEDVINLDMKGAADGSNSHFTLNPKNYDFEYEDELDNIDDIPDAEDFLNSLSSPCTVKDLHVAQKHLGVRLPLPVLQYFMVHDGQEIPPSSTRKIGIMYGLQLLSLAEVVTMTNYWRKVSRKESNARYAAQDSFAKEEKQQERALYEKEEKLIDNDPNFQIVKKIDRISEKDLQNYPDLVRNMSTHSKNDYIKKLPDQKSYPPKKVKLQYANPNWIPLVTDNTGNHIAIDLEPDTDGIPGQVIIFGREYDTKYVIADNWGQFMNKFALDFEDAGRCNLDYDSEMGQFDLAYLDKKGEIVDEGYLYVLAQRTLGKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJL3</accession>
    <name>A0A1E3NJL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apurinic-apyrimidinic endonuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_11309</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017414.1">
        <property type="nucleotide sequence ID" value="XM_019159779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJL3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJL3"/>
    <dbReference type="GeneID" id="30176466"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="10">
        <location>
            <begin position="20"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="320"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="352" mass="39621" checksum="F3AED9B061C80053" modified="2017-01-18" version="1">MRFGAHVGMGGGVFNAVTNAINIGCNAFALFLKSPRRWESPAISAEDAETFKELCKKHNYNPRTDILPHGSYFINMANPDEEKWQKSFDCLLDDLKRCEQLDIGLYNFHPGSHLGIADTASHETAENAGVRRLADGLNRAIKETNFVRVVVENMAGHGNYVCGNLDDFHTLFKLLNDPKRVGVCIDTCHAFAAGYNLNNEEEWNGFWKEFEEKVGIENLAALHMNDSMAPLGANRDLHQKLGWGFLGLECFRLLANDKRLEGIPLILETPLDGNDEGHYGEEIKLLEWLVGKEKDDPEFIAKSEEFQKLGFKSREEQAAKFEKKKTVKRKNEGKADISNMMKGRAKKAKKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJN5</accession>
    <name>A0A1E3NJN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_33415</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017468.1">
        <property type="nucleotide sequence ID" value="XM_019162822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJN5"/>
    <dbReference type="GeneID" id="30179509"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF4">
        <property type="entry name" value="SPORULATION PROTEIN RMD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="365"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="607" mass="69459" checksum="38B5135567400376" modified="2017-01-18" version="1">MLSTASNTKKNTAGPAGPPVSTAKRSPSILVTDVRKNQIRAPFAGHKLEQLKKKKEKSSERVSNLVQSMRRSSNSKYSNISVSNLTSNIGDIPSSRQREAPRLVPSKLFDSNNFYQRIPQYVKNLPSRTSKITQKLVLIPENGSVSSEDSREKSTTENIFQEVCNRTKAEKLTKEQREHEYPRVTAYLIAEGFNLKLTSKFLAKYHMVLPRLYDEVLYIPYSLPLLPGENGYRVQSNNSLKMQRGIKLMEHFINKSEERDHHYEFYSGADNENNITSVESGNTITNSPNTNSDFDPSEPQYFVSSSPTESVIEREIRDSIDEDRSNSTESTQSSDTSMRAELNTKHQQSTDERKLFSNLSKHAEMFILDYGVVVFWNFSEIHEKNILADLVFAKIQPEEEELSLIVKPVPEHDIETEEFHFEYNRDVPTPRIYNDMITLKSGDHLIKLTMSHAIAQSTKLSLFEDKMSTILNSISKLPKALAMTGRLQNYTTKRLLTKTGRLFQLRSEVNLLSNVLDTPEYFWSIEPGLHPLYTAVRDYLEIEQRVEVINDRCKVFLDFFDILSDSLAERKMTKITKMLIVAIGISVIVSMVEIFVRYLIITKFTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK03</accession>
    <name>A0A1E3NK03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">YjeF C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_21701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017580.1">
        <property type="nucleotide sequence ID" value="XM_019162218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK03"/>
    <dbReference type="STRING" id="763406.A0A1E3NK03"/>
    <dbReference type="GeneID" id="30178905"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="317" mass="33921" checksum="FA0D2745E4FAD893" modified="2017-01-18" version="1" fragment="single">LLPPLLPSFHKGQLGKLAVIGGCEDYTGAPYFSAHSAMLSGVDLVHVVCEQAAAPVIKSYSPDLMVHPYLLDSESVRMRVKQGSGSAEEVMAAQMEKIVGLVSRVDVLIVGPGIGRNDGQMKEFLLAILEHVLAKAERKDVAVILDADALYHLSVNKRLQGLVQGSGNPNIVLTPNVVEFQRLADAFGVSVDGPDSDRALGELCRTLRCTIIQKGTVDKVCYHGGEVLRCGDPGSLKRVGGQGDSLTGMVGAFLCWGTGAYRRGLYETPDTLTDEQVVSLACLGGCTTTRRAGRLAYEKYGRAMLTSNLHEFIGDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK33</accession>
    <name>A0A1E3NK33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-gamma 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16336</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017559.1">
        <property type="nucleotide sequence ID" value="XM_019161172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK33"/>
    <dbReference type="STRING" id="763406.A0A1E3NK33"/>
    <dbReference type="GeneID" id="30177859"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="83"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="6">
        <location>
            <begin position="248"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="46317" checksum="1FF62A6483034102" modified="2017-01-18" version="1">MSLGTLFGESETRSILPVGLVKAFKLDVKLEARGSAAHKAAGFSTEKVPAFLGPHGYKLQESVAVLFYLVNLHDPESPLLGTDLETKAQVLKYISWGTAELMDPLTNIIRVLLARIPFNKKLLDTSNTEVDNYVVELEKRLVNHTYLVGERLSLADLFVASCFYRCFTLTFGKKWRSSHPVFMRWFNTVAATEYLSYFFDNVKFIDEPVAPPKPAKKEKKDEPKKAEKKAEKPAAAAAPEPAPEPKKVKHPLAELGNPTIALDEWKRVYSNKDTRKEALPYFWNTFYNDDEWSLWRVDYKYNDELTLTFMTNNLIGGFFNRLTGSTKYMFGCAVVYGENNNNGIVGAFLVRGKDSAPAFNVAPDWESYSYTKLDASKPEDKAFVDNMWAWDEPVVVAGEKKEIADGKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK34</accession>
    <name>A0A1E3NK34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_131094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017617.1">
        <property type="nucleotide sequence ID" value="XM_019160018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK34"/>
    <dbReference type="GeneID" id="30176705"/>
    <dbReference type="OrthoDB" id="3998070at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="370" mass="40692" checksum="2C46C0F0EF811FC9" modified="2017-01-18" version="1">MEKSIVTSFITKDAVQGGGEATRSGGLVASSGLVMHKNTNGNLEIPTANGNPFLESTRFRQGTLYEIIFPIIGGFLFLYVLGVVVNKYKASRQAKVVEPFDKDYEFTDYTGPELRKNRGGHGEDELENPFSDRYESSNKSFTHKKNLSSVDFISQYRRSMDHLSGVTAAPPYSNHHADDSFTSLSLQQHPGLQHSKSQSIGSVLQLNFSQQPQLAPQSQSQNNSSDPDNSVFYSMLGTESPAAPNDSTKAETDSKENNTDKTSFYSIAEPTKAAKNATVNISYENGTSSNNPIMQNARNNVSVESHSMFEDTSFNNSYDIPESDYAVKHAVVRSPSPRRNNHVGMYAQSSSRSPTRSPVRSPNRGAFNNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK40</accession>
    <name>A0A1E3NK40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Histone deacetylase</fullName>
            <ecNumber evidence="2 9">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_73415</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 9"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017041.1">
        <property type="nucleotide sequence ID" value="XM_019164473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK40"/>
    <dbReference type="STRING" id="763406.A0A1E3NK40"/>
    <dbReference type="GeneID" id="30181160"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045835">
        <property type="term" value="P:negative regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032874">
        <property type="term" value="P:positive regulation of stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000007">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF36">
        <property type="entry name" value="HISTONE DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="13">
        <location>
            <begin position="59"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="467" mass="53548" checksum="CF917E8BAA981E71" modified="2017-01-18" version="1">MTLASWMDESSSFAPDARTAKHTPFFEPVVNSFNDGGYRPNVSYHYNPNVSAYHYGVRHPMKPFRLMLTDHLVIGYKLHEQMDLYETRRATEEELLNFHSADYIEFLQRITPENSTKFADDLKKFNIGDDCPVFDGMYDYCQYYAGASLDASRKLISGMSDIAINWSGGLHHAKKYEPSGFCYVNDIVMAILNLLRVHSRILYIDIDLHHGDGVQEAFYLTDRVMTVSFHKYNGEFFPGTGNYDEVGVGKGKHFALNVPLQDGIDDESYVRLFKSVIEPVIAKFRPTAIIQQCGADSLGCDRLGCFNLNIKAHGECVKYVKSFGVPMLCLGGGGYTPRNVSRLWCYETSVLANVELNSKLPDSLPFRSWFQPDYSLHPNLGDRFENKNTKKYLDNLRVQLLENLRYLNQAPSVQMQEIPPDLQNITDDKEAQLVDRIKRESEIDKYKDAERYYVNKKDESRRGELLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK58</accession>
    <name>A0A1E3NK58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Adenylyl-sulfate kinase</fullName>
            <ecNumber evidence="4 12">2.7.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16378</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.25" evidence="4 12"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017622.1">
        <property type="nucleotide sequence ID" value="XM_019161184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK58"/>
    <dbReference type="STRING" id="763406.A0A1E3NK58"/>
    <dbReference type="GeneID" id="30177871"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00205"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000212">
        <property type="entry name" value="Adenylyl-sulfate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF1">
        <property type="entry name" value="PAPS SYNTHETASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="13">
        <location>
            <begin position="24"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="199" mass="22481" checksum="431FC96D0099562B" modified="2017-01-18" version="1">MATNITWHHNLTHDERKELVKQNGATLWFTGLSASGKSTVACALEQKLLQEGIQSYRLDGDNVRFGLNKDLGFSEKDRNENIRRISEVSKLFADSCVISLTSFISPYRADRDAARKLHEEANLPFVEVFVDVPLAVAEQRDPKGLYKKAREGLIKNFTGIDDPYEAPEHPEIHLNTHEQSVEECVSTIVAYLKQKQIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKB3</accession>
    <name>A0A1E3NKB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RIC1 C-terminal alpha solenoid region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_73277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016886.1">
        <property type="nucleotide sequence ID" value="XM_019164431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKB3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKB3"/>
    <dbReference type="GeneID" id="30181118"/>
    <dbReference type="OrthoDB" id="67540at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034066">
        <property type="term" value="C:Ric1-Rgp1 guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040096">
        <property type="entry name" value="Ric1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009771">
        <property type="entry name" value="RIC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746:SF10">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746">
        <property type="entry name" value="RAB6A-GEF COMPLEX PARTNER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07064">
        <property type="entry name" value="RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIC1 C-terminal alpha solenoid region" evidence="3">
        <location>
            <begin position="871"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1063" mass="122440" checksum="9A0CA5DD60D5A154" modified="2017-01-18" version="1">MFWPTSISQFHKLPEVESLEELGKSNPDHTYKPNPHEDDIVNILTNYSNGLPYVVITYRSIYLFDWTHKAPVNVHIRSDFSVEKFGSNLTVKLSPNQQTYAVLTDKNVILVYTIKATGSDSELLGVYSKSGTIIQNGYPLTEYEDDPYVYGGVNSKFSSKAGDQKNGIVKNIISTFIGIDGNEVPIVDLGLRLKLILNVASPIVQYCFIGSMELMLINSNPHAFQIIHLNSKNSIDKFTSSDNNQSSTESSNEINFILADELDWFQAPEINNKSEISDMNYNYDLDCFLWYNKQGDVLLVKRTGSTGDFELSAKCVYKCETNNEKAIEGIINHFKNVCYILLENGKLLIYKLNDDLTCKLLKTVDKCIPSKTPKALYLSPQGDSIIVVYDNGWNIYSALGNQNFSTFEYDHFSLSHIKKLQFLSERELLVVNQNNEIIFIDLTVYNIGQGFNSLSIKRPILYDKDKIMVFKAYEKKLVDHHHYNYNVNDVTNRDTDVWLSEMLPLRFRINNNIIRSCSVSDDGNNVCIIGNYDVIVFSSFSRRWKFLEIPQESATFEKTEIPMKKCSWWRDYLIVGSSSGKEKKKSEVVIFSDRILSEAESFCFDFIVWSFNLEDTSFDEVFLDFNVDIYNDQLLVMTNALNCYTWRLKLKTSATTKVEGDHQIKKSTMHDSSLTSLKSSITLEKSTVYQLKTCFKESDESIALNYGTVLKITDTDILFLTNTDLYYIRRERTPGSMSFAYVTYLISSCVEYVHKLNNSLICLFDGSQLIHYNLSDNINLLKLKPIRISIGNDISQNDGGGYSVKYTGTCPYPITTISFQNIMFGIEIDCFNKLKLKLETSRRNYLTDLVNHYILSNISVKNLEDDSNAMNITTVYGKFSKFKNFKFVLEKLMVDYLQNCYEDPKFDSDNDYFNRIYSLINLTGNSYEIVLNCLKKTETHFWPIFFKKSNETPRSVVNKLYTDCNDHKLSAHFFIIMLNYEKYDYSSEDNECYPGESKPQTSKKESCNISAEDQDLIFNILKRLILSGDFETSFELVRFLKIVDDKMTHKCLVKMKKYLKLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKH2</accession>
    <name>A0A1E3NKH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_146007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016946.1">
        <property type="nucleotide sequence ID" value="XM_019160453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKH2"/>
    <dbReference type="GeneID" id="30177140"/>
    <dbReference type="OrthoDB" id="3992305at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="868"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="877"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1146" mass="132599" checksum="F65BFA291B62D2D5" modified="2017-01-18" version="1">MFSKVRYRYVPRRGSLIFQFRHFSYLDSKDGSGSSPSGKPRNGQTQDRAKPEEWKSPMPNNGSKFSVAVSSFLNSSLSSLEDYIANQAPIKSSQQSEGYNTELLEIDMDNDVTPQKSIGNIPYQLGEINKGFKEFIDLSNPDHIRGLLAINRERWIKTSPEISQAIDKTCNVDIPLILNFYLRSSAEIRRQLQDPSMNTKVGMTSQRHIISLLLNLQWFRSASFIILEMKLPFNDLVNYLNELLEDELIFQKSDWMRYLFIINFYQLYKNVNPLVLTNLDFLGRPQYMDRFKILNMMWRTHYTTKQELKTDVSEWYEMCGHDIPVSLMHIYKSVDIIKYNNPFNLAGQVNELKTLIGASSSSLMLQKNFLASFLKHLVSASDFELQYSQSPQCNLSKKEVRDFLTKRKNAIINFVVLSKKLQTKSFDCLTVLELRPPSAVGLQLWDYHISGMNNSLSSTIYYKFLVSYFVSQLTKTSKINEIVKVEFSNLSSARKTNLITTSLISYLKRSDTLTRNRVGGYIALTNQQREHSHVMKNAIQKIFSKHENYKFNYSDLLKVFELVSSFSPTLSNKEGLFEIGNQIIEANHENADFFPFNAQEKFTKTIEFIDLLKSIDPESKIYVTRLVPSIINFYHEFILSADDCAIDFTKMPEVKQLCYILNKTFDVACRNPRELTLLNPKNYASKIYITNQGLLQKVSSKLFQLPKNVLFFILQSRATWICADQEDWISKFHKAVFTFGIFLEVLVRRSTRESYEGNINFSELNDVQFAKALKDEMDTISSNETVWECVEMLSGFEWNEEVSDVFFRGKSIHSGETEPEIGFGIIMVLTEVRKLNGTVYSKRCEAIEGLMQKDTGNNEVESFDDVTDALFDSNGDSPHEDDHLLDTTKHSDSERDNEQETNVTVSRGLLDQDLLEFNELYRNMKNYEQFVPHRNKGTGSEEQSVTIAKLSKSVPSSKVKKRYTVREKERLVRFYSPLRLKGMLIESLIMRNPKLIDAIVRRLYVEYETRIPVSLLHSAMIGIIKSEQREVGFIEKINVIKVLDTLASLIYSTASHKVHSYLFMNCVRFREFRIELVDMIIEESKRSNSGSLKTLNWAVNKITNTPNMIQYKNEFARWTNDLNNMKEMKVGFWNPGNTGKWIKDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW3</accession>
    <name>A0A1E3NKW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_148228</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017076.1">
        <property type="nucleotide sequence ID" value="XM_019160549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKW3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKW3"/>
    <dbReference type="GeneID" id="30177236"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051643">
        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF19">
        <property type="entry name" value="FI01119P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="682" mass="75949" checksum="D77AFF4FA87CF4DE" modified="2017-01-18" version="1">MFSSFLQLLFGEFSSLFSYLSPLYDLVKYVSAPSLLSGPKKGQGQGRDAAGEVGVASAGSTSSTGATTVDDNDDDFGSDLFSKPSTYKNFSSSSKTLHQCAVSEFDYDFTSPNPFSWDFQHLVLANQLNSNNPSLNPNGGTNNKQHITKGDIVDENLSANNLGNPNVVGATSVNSISSNNNIKNNDATALNNINTGNSDEVPVSTNLLFNNNNNGLQSNYNNNQNLDGREASEILSQFSASFQNQLKRNELYQIYQKQNQSHHSHEGPSDQLRQGEQRIDLTDIGIQKSICAEEAKDAHISEDEAEFSDDDYDDYLQSSYQPQISSSSTHPPLSHSFVQLNHNPVSYHFDSENEDDDEDRDTVPLDEELMLSFLRRSNDNTLSVYYMNSDLSEGNTVSPHTATSDSVSGSSPTEHANILKREIEPDAAFGKHSKDRSMKVENELINNEQSISSFADLKPNQQLIINNDNNNIEPQKKYRKLAFSSKPSMSLNMKDTSSTVTFNNSSSDGNNTLPPTADFKMNQSLDDLQRSDKLYICPICQTTFKVKGYLTRHRKKHFASKPFQCPYFDPTVDYEEGTDNDMIIHQHGSDCEDEEVSPNGISDCSKSKVPRCHPTGGFSRRDTFKTHLKALHFVYPTGTKSGDRSDKKGRCAACFKEFKTNKEWLETHVMTNQCEGMITKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKX8</accession>
    <name>A0A1E3NKX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b mRNA-processing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73461</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Essential for the assembly of ubiquinol-cytochrome c reductase. It has a direct effect on the correct occurrence of the Rieske protein, core 4, core 5 and apocytochrome b.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CBP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017096.1">
        <property type="nucleotide sequence ID" value="XM_019164482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKX8"/>
    <dbReference type="GeneID" id="30181169"/>
    <dbReference type="OrthoDB" id="5576752at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012420">
        <property type="entry name" value="Cbp4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07960">
        <property type="entry name" value="CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="125" mass="14324" checksum="CCA98634BF00A7A2" modified="2017-01-18" version="1">MSSPTMLMLKQTAIWGGGIVGLGVFLFKFTVPTEEELLQKMSPEIRAQVERNRELRQQEQAMLMEIVKKTADSDQPIWKTGELYNPWEGTGSKLLLDKVNFEKEQAANKLKSELEALKEQQKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLB5</accession>
    <name>A0A1E3NLB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_139330</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018048.1">
        <property type="nucleotide sequence ID" value="XM_019160141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLB5"/>
    <dbReference type="STRING" id="763406.A0A1E3NLB5"/>
    <dbReference type="GeneID" id="30176828"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="219"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="397" mass="44049" checksum="F076758BD4CF0870" modified="2017-01-18" version="1">MVGELATETERAGGVPASASTLDTRDSKERGVLSEYTGTGAGRTAVVAWLVSVPKHRTEEGREGREVPSRNYEAWRASNYMYMCGGRLRTALYCPQVVRRLRVPVLPLTVGLAVVPVVLCLVFEAAWFWHEVSPAVVVLFAYCAVQMLASVLKAALLDPGALPKNVHVTDGVEKDGLPPEYHSWIEMPGPQVLRPVVPPALAAPAGAPPPPPQRNTVYMKYCDSCYIWRPVRASHCSKCGVCVANMDHHCPWLGNCVGQRNYWYFFNFLAFAVATSVYLLAMSYYKVAHAGLHASRWSLFLGVYASCCLPYALLLLVFHVCLGLTGITTREYFGAERHEGNTQIQDVCLDPFNSGSSLFNLRRQWFHGRGRANVEIRGRYAPNDLRYKELHVGTLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLD0</accession>
    <name>A0A1E3NLD0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72962</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018052.1">
        <property type="nucleotide sequence ID" value="XM_019164344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLD0"/>
    <dbReference type="STRING" id="763406.A0A1E3NLD0"/>
    <dbReference type="GeneID" id="30181031"/>
    <dbReference type="OrthoDB" id="1232at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019340">
        <property type="entry name" value="Histone_AcTrfase_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="689"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="764"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="689"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="705"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="769"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="819"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="870"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="886"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="904"/>
            <end position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="994" mass="111754" checksum="289C5113F696E2A5" modified="2017-01-18" version="1">MQQRLRSSPSAGPPTSPGRSGSISKTSQEAQSASAGKYVPSEAAGMVNGGHINHSEDILNSFTEALESANLSYDSQSGKLTGDGVQTSPTETQLTQLRLLLLKLRRKIVVELLRQGASISAVRACVESYSSQETADKASLSDRYDPENNKTGSANKDNSVSDDIAGEEGRDATVIKIGGDGDKIHIKLEKRSINPDDKSKKRKHILVSGDEDDVDDDHNGSGSRKDDATNTPISIKFEDNDNDDDDNDFKIDLTSAIDSDSNKIELIDDPDDEGLRPRKKIRVKLEDDEEESIKLIISKDRGTGKEDDVFIPSVYRSDNPDANEILNMDELVKTSMKELQLYDDEENHKTARDEVEYKKRKFAVSEFPESDLKDMLPGEIPMIDFSGPKPTQQVAWNSFTAYIEPFYRNFTEDDVKWLRQKCVASSFLSKYLLANEAFVVTENSEDANAENKGILAGNGIKDARPLDNTYNPYYIPPLGPKYSDVWMLENEGKPVKMGRLTQSLASKMKKLYAPKGSPVNFDANKPEALVALCEEQDKVSCGPLTSRILSAIVSDDEDDAFELDQSEHTDGENGEDSHNDASTFKRDDKISFEQLEKRLNRELKYIGVFMNVVQTLNNSSWEQDWALGKEDDEVSSELRILQNELTKVQKRNNFRKEILVPIVEEQIAWQEYMTIVDDLDKQVEQHYRRRLNVTPKKTKKRGPHSSHSGGDSSSSGLMKDDAAHLVSNASFRGLLDKRRRWIEKIGSLFKSPLEMRRMPKHTVFKVAKEEEEEEEKEEEEADGEKEGDDVEVEVMVVEEGEADSEKEKALAEGDDEEEGAKQKETNDGEKSGDRNENNVDNGEKKNDNEAENVEKDSMKEKDTTAVATEKAIEKKADAKEVKAEANVESATATATATTATSTAGDEEEEDDEENEEEEEDDENDEEEDEEEEDDEEEDEEEEEVEEDDEEMAAIEEEPEEDEEDDDEQDDDDDDDDEENEEDDDEEEEEDDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLF6</accession>
    <name>A0A1E3NLF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_139873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018088.1">
        <property type="nucleotide sequence ID" value="XM_019160170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLF6"/>
    <dbReference type="GeneID" id="30176857"/>
    <dbReference type="OrthoDB" id="5408025at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="23925" checksum="58BD54F70605DA35" modified="2017-01-18" version="1">MSRRPRPRPRPKPTVTQRGAMTSWSIDAGSIDPGRAVQQSQPARGLAACCPLPEHSDKRGERVTAVSEKRETQSNMDPALLLCGLHNSSALAYPPAPPLMTMLHTRFLLSKGFGWQDDLEFCPVVLDAAAAPGDNASGAKTAADSSQHYFVPTDAASAAAYTLQHSPPSSAASSAAAGSASTAATSPNTSLSARTASPLIHTPRAKKILDIVNPHTGLRVGSPSARTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLZ4</accession>
    <name>A0A1E3NLZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_10130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018209.1">
        <property type="nucleotide sequence ID" value="XM_019159634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLZ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLZ4"/>
    <dbReference type="GeneID" id="30176321"/>
    <dbReference type="OrthoDB" id="76676at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="342"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="572" mass="66653" checksum="D0538AA95A1A282C" modified="2017-01-18" version="1">MSNPAAPSTSIANVKLEPASKDSDYDVFPLRALTEDEIDETRYHILKFHSAAQKRSEELTISNVVERVGTKKKQDEEKEKEQVVKREEFLKRTGKKDLTDEQIDEELEVEGLDERERKIYDDKKKKELELKEKEEEKDRQMQLVAPDGGARKQKKQLIKKRTKQVRAYNEKKRKLRYEEFYPWVLEDYDANQAWVGNYEAGINDNYCLLIQDTVNKCFKLAPVEKFYKFTSRNKYATLTLEEAEAKMKQGGSGQRWLMRKMHDEATSGKRVDLRYRKVQKVKSEGGDEDDEDDIGKRSDEEEDLDYDDEFQDDEEAPIMEGPDDEKKLIESKMKKEMLKANSLIEKVPDEEEDDEFDELFDVKKEDKESKKLRKALSRNAMNEVYASDDEEENPYLSESEIDHESDSDSTDSEKKKDGDVKVKTEPNDDDDDEIKVKSEPKDGDDFTGETASPKKKKSKSKAKISVESYRSGFITIRATKQILSQFPKGEWNPRTAIRRDLIDEEITNISNPNKLTREDIDSLLVNGPIELSFLVKSLRGKMQRDPNSKDDLRQILKENFILKNKQVHRRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM84</accession>
    <name>A0A1E3NM84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="10">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_15228</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018360.1">
        <property type="nucleotide sequence ID" value="XM_019160725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM84"/>
    <dbReference type="STRING" id="763406.A0A1E3NM84"/>
    <dbReference type="GeneID" id="30177412"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102262">
        <property type="term" value="F:tRNA-dihydrouridine16 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102263">
        <property type="term" value="F:tRNA-dihydrouridine17 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="18">
        <location>
            <begin position="45"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ47247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="446" mass="51223" checksum="B1E2AAA378B8A3C8" modified="2017-01-18" version="1">MTEQAENLTNKNNTSVTKEKSFDLEASVKAGRLLFNKLGQPQRVVAPMVDGSELGWRLISRRYGAQLCYSPMLHSRLFSQDKKFRDQYLCEMDGDPELDRPLIIQFCANDPDILLEAAKHVVGKCDAVDINFGCPQGIAKKGHYGSFLMEEWDLVYSLINKLATELGDQLPVTAKIRVFDDWSKSLDYAKLCLNAGAKFLTIHGRTREMKGQKTGLANWKLVKYLRENLPEGTVFISNGNILYPDDIERCIKDIGCDAVMSAEANLSNPGVFWTKSNDKEKLFPRVDKFMREYYDIVKSCKGTESKRCMKTHMFKALKTFLPYHTDVRAEIAKLTKNSSFEEIEKVIVMMENIVEEIFKKENIEELDEIKVGETQEWGGRYREVPYWRLQPFFRKVDGIEGKELVKGALEEIAEKTKQQKALLEGKKRKLQENEEDHQSKLQKVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMD0</accession>
    <name>A0A1E3NMD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_49875</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018397.1">
        <property type="nucleotide sequence ID" value="XM_019163143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMD0"/>
    <dbReference type="STRING" id="763406.A0A1E3NMD0"/>
    <dbReference type="GeneID" id="30179830"/>
    <dbReference type="OrthoDB" id="10027144at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043577">
        <property type="term" value="P:chemotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045141">
        <property type="term" value="P:meiotic telomere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02394">
        <property type="entry name" value="KH-I_Vigilin_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057778">
        <property type="entry name" value="KH_Vigilin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF31">
        <property type="entry name" value="DODECA-SATELLITE-BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24668">
        <property type="entry name" value="KH_Vigilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="160"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="629"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="704"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="777"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="854"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="935"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1005" mass="110067" checksum="125CEA4FB131A1A2" modified="2017-01-18" version="1">MSTPAELLALKHAQASAAEEPSSPAPTEFSTATPNSVEDSVIRMDDDAYFPSLGDATGSAPALWGPSSDPTRSSWAKPVSSFRPAVKSSSTQVTFIIADEQQQNLAKGELFKIFAKIQASLNVKVESTYSGATKKRTFLLNGPSDNVPQAKRELIRQLTKPTKISFEIPSKLRSAVIGSGGKTLKPIIEATSVRIDIGRESETSESSNSLLTEDEELFGKSLTVTIEGDIQGCSDAKAKILAIVNEHTKTLSVRVPVSEKLKPFVTNEIAETVFPEGVEAICPEPSSRISYILVTGPREGVIEARNAIKSTLVSLDNQVVVEEREVPKHLHPFLDTDKILEATSVVMEVPPQDETSSKVKFIGVKSNISKAIPFAKNLTSEYFVDSLDISKSHGGNYQHAKYLTAFFAYTKYFDALSSQYGIKVSGPSYKNIIDPEVKNVVISFSSTKDKKDDIKKARKDLVDNVNKITPNFVKAVSDIESFLFPKLDNTVPIENNVSVVPLGSLAGAGNMLLLIVQQNDDEFLPSADQVEAKLNVVNESLNELRALSKDLIAETIETPAADQAHLEGSTLNVLLNKYEPNSIEIKLHQNADGASPDEIYLRGYKNEIKKAIGDIQQLIDDVKNYEEASKYNTVIEFPSKYLARFIGQKGASLNQIRDEFDVKIDVLTDDSDKVESAEIKLTGLKSNVDECIKKVSQLSKKWADEKTATLNIEQKFRKQLIGPSGVYVNRLQDKYNVKIQFSDSNETKGSVVIKGPSRGVAKAEEEIKQLLDYEKENGFVEVIQVPSEALSRVIGKSGETINDISVDTGVRIHAKTDQAKAQETGSADFEIVGSKAGIKEAKSKIDAIVKRIVNSTSETIEVDPKWYGNLIGPSGKTLREIVINAGGSETDRDFRRFIQFPARNSESTTIVCEGDKAVVAKIIDTISAMVKDFESVTDETIEVPKKQHRHIVGSGGSVRRSLEDEFKVKIFVPKQDSESDLVRINGKPENIAKAAEKIQALTASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMF1</accession>
    <name>A0A1E3NMF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone transcription regulator 3 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_111072</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIR3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018422.1">
        <property type="nucleotide sequence ID" value="XM_019159752.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NMF1"/>
    <dbReference type="GeneID" id="30176439"/>
    <dbReference type="OrthoDB" id="77564at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2013"/>
            <end position="2051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2065"/>
            <end position="2153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2171"/>
            <end position="2191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1888"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2013"/>
            <end position="2040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2191" mass="251357" checksum="C1C9169DC67B47B4" modified="2017-01-18" version="1">MSSFTALNLSDDELDIEEHTRELQIEVALNTLNKALEFQKNGLFDKAHQKYDELGRIEILQQPNISNNPTINRLKYLYCRNRGMLRLRELIDQHKSKQKRTKHSQLKDSNSSTAAKVEKSEEKSDHPFSLGENNLGTIEVVSSDKGTDYDDDELEISIYGEMIGSMNDLLNATVFGIPDVKIIKILSSLFNHFGHPRLARLCYEMKLNISEDYKSNDDLKIDLNKPEELLFNQVNLLENFLQLLNDISDSTSSLYKRVESCLHSKKFWTQNKLDAEHKKYNWNDIFTIEQWNRERLRETDILVLDISVVDGVVDLSSLFEEIINCVPKPKGKSKLHDGYYLTKSIFGKVEFEFDIDVSHSDTPSSPTEIHDKSIPAADNPENNFDVEITEEKEKIDTKDTVELKAKDVIASRATRSTRTKSDTDSANDHAKTRLKDYDIFISQTLPSFLRLCDINIEFQPLSKLVNGDYSETTEEDKIVSQFYSCVASWEDEYTQCLNVTDEKRNKLQAKSVLTPASESESIREIVNLKSAKVINPHLFKRKNMNRFFQYLQELSKRNLHFNQVRTAVVKFMFNCDLESGASILCVEPMNKKTLRNFKSIVDCVSMSFYNEFEHFTLYELNQSSKKENLISAFNNSESVFEVLVDSYVEFVQDQKLKRASTYKNTLTNIQNFENTIVKRIEKWKCIIEDAFAIMDYHDLPELAHIWSRFVWFKLYYLQSHSQVLNPQELTDVLSELSDVVKEKQIYLPFVNYELIPVLNYENIQTQSSKLKILEIFDKNEKSSKILESVLLKIPLNYSHNGNEIQKIQTQFENFIESSNLPLKLRLWSLLLRYYRLNNDIFKYKNAFEHIVDIMASQLRTENLNKLAVHQAKILIFNVIGFFSHFSHKFITFCNTTHFECFNNVDRESSLKMVSSVSHFLYMLYSFLTYQNAVQIDSRPSLSVRSSRAFEILNNGVSSCFFLLAFYYPTALSNPKPEFIGDFLSICHIELGLLHMCSAVDGAFLKYLQYKLTALNFDISANDVFQIIHCRFGLSVSLDGFETFDHKCKPKKMTIEDTIQLSKFVSTYCFKGKHPVLSPPRNDIKSIIDKIVDVVGDPDLANPTVAKNRSILDSYLRKTNINLQFVINSFQGKSDLNFEKPDVPGIEVSENGLYYIEGLIGLHFFKVRRRTMQSRAAELDYVLKMLENDILCGCNRFETWVALGQTYSFLVEDDLIWTADKLNSVERKQLTSLTQKKALLCYFMAICVHIGASEEEKARHKLVVPILWEAFAKELYGTWMEPMNKKAFHIYVDDDQHDSRVVSNDSLSNKMANLKPQTNDIPNNAVFKLLELAFKSAVTEDETNWYDVMYLAKSQCKLNYQNIEPKIIIENFLKSCDLAMRQSSKDDPIIEPHYYLFSVISKYYRSSKISIDEAIGYINQDPLFAAIFPSDSSQETFKSLTLKVLSKIISYDRKNWQHRPVYRLARSFYDMDHDIDSAKKELLSLINLKPNIRSLSTIWKPTSERPGKHFIYNSIYTQFLVQLLYEKGDIYSLTILLKKMRRAGSIMVNLTKTFDNMMLRICILIKKSLNLLPGFLDDSISKLKFSDFTKYSALFIEYLETITMGQFDDDTSLHLFFLSETQAFRKLATGFGATGLIDECYHSIYIKLFMPFLFEKLIAEKNGGVLDKNLLNTAKGFAVERLARSKQSTPDLTVRFNRTASDLESLDLKSASLVTNTDESSIKAPHPIGLDEQLREVKNSLEEPVECRIEDGLTPLSASDLSMKERYSIINYLSFGKSSITGTPSKEKVKVARRDVSPFAMKVVNVTQALVSSMKENSNDGETIDYKIYGPLDNTEFEKAIVGVMVKETKENKEEREFDELISAHDRSFTEDELIDFNQILENFQIQKLSNSQTKIESENIQKAEVETQKAKEAEIELQRLRAELNRDINLSETSADASRNATPVPVALGVLKQDIHSCESYSSSVEYNPFASPLKNTEESTKDIIEDSLRENRHVVEHEKLAAVKNLTQSISAVHSESPANEETRLTNVLKTEPQLQSGDKTSEKTPVRTTQSVITSFFRISPNKRLLSPSDASDENEVKRRRAKEATPDQQLGGERPETPDPAVQPSFIHSLESDSGLEQTKRTEPTISPSKRHSRRDPARSALIELGKSGDAREQVDIKLRKEHALLEQAESLTSSSASDSEQSVIVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMI0</accession>
    <name>A0A1E3NMI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_21250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018452.1">
        <property type="nucleotide sequence ID" value="XM_019162190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NMI0"/>
    <dbReference type="GeneID" id="30178877"/>
    <dbReference type="OrthoDB" id="566238at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01519">
        <property type="entry name" value="RHOD_HSP67B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="29"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="124" mass="13722" checksum="DE8446C790BC01EE" modified="2017-01-18" version="1" fragment="single">QSRQYSVIDSDAAAPNIDFSQMKEIASSADPKYVIVDVREPDEYSAGHIPHAINIPCKSSPGALGLDPEEFKLTFGIDKPSAEKTLVFYCLAGVRARMSEELAATFGYENRLNYIGSFKDWLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMJ6</accession>
    <name>A0A1E3NMJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_15290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018434.1">
        <property type="nucleotide sequence ID" value="XM_019160753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMJ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NMJ6"/>
    <dbReference type="GeneID" id="30177440"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11819">
        <property type="entry name" value="SH3_Cortactin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF42">
        <property type="entry name" value="HIGH OSMOLARITY SIGNALING PROTEIN SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="ADF-H" evidence="5">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="500"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="587"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="367"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="656" mass="71434" checksum="6B252D9468E5DDD0" modified="2017-01-18" version="1">MEKLDLSSYAKDIKHNYDAVVSGDNTYAIFTTLKDSSLKPTVLGDGDIQEFVSEFEEGAVQFGIIGVNPFGSDVKKLLLLGWCPDSAPIKSKVSYAQNLSAVGKVLHYHVEVTARDSDDLDVDDLLKTVSNASGARYSIQSLTPQKSASSSFKQKPKPVSASSSSSSSSFVKPKPAVSPSIIKPKPSLPPKKEISGSTNDWGDDGEIKERDFSKEPLENLPSAYKPTKVDINALRNGPGSTTESEMKTISINPSGGLTSLPKPKPSHSVLSRFNQEVVKPEFGTKTPSFGSNPSKDSSKLVGGLSRNFGAENGKTPAQLWAEKKGQYKEVPNNDKPVNAEDIEKEVDHEDISLNSAKAKFEKLHLEAEKEEEVKKPITSSISSFPPPPARNEPVKQFPPPPSREEEEEEEEEKEETSNPNLISSFPPPPKRDVFSKFEEKPAEDKEDDDWGDDDADSVNEQHKSFQPPASSTAPAPLPVRPIPAAPVQEDEPATLLSSSAGGKHAIAEYDYEKDEDNEIGFTEGEKIVNIEFVDEDWWQGTNSKGETGLFPASYVNLIEASSSAAASSEPAPPTLPSRDQAVEEAKSSGLSAVAEYDYDATEENELTFKEGDIITDIDKVDDDWWLGKLCYATMNDGICLCFLSCNASLLPPKCYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMJ9</accession>
    <name>A0A1E3NMJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
            <shortName evidence="7">Glu-AdT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HER2</name>
        <name evidence="9" type="ORF">PICMEDRAFT_31761</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018458.1">
        <property type="nucleotide sequence ID" value="XM_019162717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMJ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMJ9"/>
    <dbReference type="GeneID" id="30179404"/>
    <dbReference type="OrthoDB" id="421993at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="35"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="471" mass="51816" checksum="9DB4AA6A50290D18" modified="2017-01-18" version="1">MKANITQVRSVNKRFNIFTSLRTNPTPPDPSPYAPLGNTTYAIKDNFATRKEPTTCSSKMLEPYISPFDATVVTLLDQSGSSCIGKTNMDEFAMGNSTISGHFGPTLNPVYPQAQDLEQRIVGGSSGGSAAAVALDLVDYSIGSDTGGSVRLPACYTNIYGFKPTYGRISRWGLVAYSQSLDTVGVMSKDLNTLWKVFTSLDLSDSKDPSCLSDSTRESIAIERLNSKKNVGKKFKVGIPVEYQLAELGDDVKSSWKTLLNKLQASKLIEVYPVSVPSIKYALPTYYTLCTAEASSNLSRYDGIRYGYRVKEDECNTKIEPEFITTRNLGFGAEVRRRIILGNYALSSYGYDSHFMKATGVRGMLIDEFNQIFKDRNCLVQKKHFQSTAGVDMLIVPTTVSEPPSIKDFSKMDTVKSYMNDVCTIPMSLAGLPAISIPYGGNSMGFQLVGQHGQDYKVLEFARLVEEVIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMS4</accession>
    <name>A0A1E3NMS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_112170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018489.1">
        <property type="nucleotide sequence ID" value="XM_019159770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMS4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMS4"/>
    <dbReference type="GeneID" id="30176457"/>
    <dbReference type="OrthoDB" id="3992998at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="255" mass="29765" checksum="D36BF9861FB699BD" modified="2017-01-18" version="1">MTTRTVNNFKTLREILDYNDRLVQERNLQEHHEELTTESNSAKTYTLKKTTALSVLNWDAVFSNQKVFACMMLMLITVLSQLVFSYVTFALVNVQQSLGLFYLSQALNLFFSYVMPLSLIKEMDEVFETPVAQLSSEIEIYRQLKISVMIYCVCTIVSAAALASVFSSGPFIELRYNFQELRDVPVDLTSFVRVLQIISFQLTLINLILCSINLYITDRKIRRCFNRIERLQDRADLEIMFNENLIEQRFDNNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMS6</accession>
    <name>A0A1E3NMS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_23622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018543.1">
        <property type="nucleotide sequence ID" value="XM_019162316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMS6"/>
    <dbReference type="STRING" id="763406.A0A1E3NMS6"/>
    <dbReference type="GeneID" id="30179003"/>
    <dbReference type="OrthoDB" id="10267127at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00144">
        <property type="entry name" value="MPP_PPP_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050126">
        <property type="entry name" value="Ap4A_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850:SF4">
        <property type="entry name" value="ZINC-DEPENDENT ENDOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="297" mass="33593" checksum="C8FC756F41A2934E" modified="2017-01-18" version="1" fragment="single">SNKTKKKRLILIGDVHGCLKQLKKLLDYVKYDGGEEDHVILLGDFTNKGPDSIGVINFAIEQKLDCILGNHELAMMKRYTQFHGLKSPTFVNNDEDTNSTLTMKESYDLDELMRLAKKITPEHIDFLSSCSPIKKIGPVPLYTNVKQSRHASSPADGIAVHAGLLWHKDIYHQDIEEVTTIRNLLPPDWKTPTEDRHDKLGGEKSVAWTKIWNKHQLEIYNKELKEADNKNLTIGTKVFYGHDAKRGVVTKQFSNGLDSGCVYGKQLTGVVIWSQVGSVNNEEEKIVYKQMTVDVNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMT7</accession>
    <name>A0A1E3NMT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">biotin synthase</fullName>
            <ecNumber evidence="4">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_139204</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="4"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018041.1">
        <property type="nucleotide sequence ID" value="XM_019160132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMT7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMT7"/>
    <dbReference type="GeneID" id="30176819"/>
    <dbReference type="OrthoDB" id="2414104at2759"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01278">
        <property type="entry name" value="biotin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="82"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="413" mass="45881" checksum="D1A2B1B6C4550999" modified="2017-01-18" version="1">MMSRALLSCFYSFRRTLATNAANGKVGGGCSSSPAEVLNLALTTKTPITSWTRDQVAALFGMPLNDLMFYAQVQHRQYHNPAEVQLCTLLSIKTGGCTEDCKYCSQSSHNDTGLKATKLLDLDTVYEKAKVAKQNNSTRFCMGSAWRDMKGRKSALRKIADMVRKINTELQMETCVTLGMINEEQAKTLKDAGLTAYNHNIDTSRSHYKNVISTRSYDDRLQTIQNVNDSGIKACTGGILGMGETVDDHIDFVHTLATMNKHPESLPINKLIAIKGTPIEQELKTKYKDRKLGLDNILRVISTARLLMPESIIRLAAGRYTMKEHEQLLCFNAGVNAIFTGEKMLTTMCTGWDEDIAMLKKWGYVPMEAFKKTAPREKLNVTIGANNERSVSVITGGNLELPDDKNSVKELSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN26</accession>
    <name>A0A1E3NN26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_30686</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018622.1">
        <property type="nucleotide sequence ID" value="XM_019162648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN26"/>
    <dbReference type="STRING" id="763406.A0A1E3NN26"/>
    <dbReference type="GeneID" id="30179335"/>
    <dbReference type="OrthoDB" id="526653at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021384">
        <property type="entry name" value="Mediator_Med21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381">
        <property type="entry name" value="RNA POLYMERASE II HOLOENZYME COMPONENT SRB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11221">
        <property type="entry name" value="Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="137" mass="15595" checksum="4F7171312AC70725" modified="2017-01-18" version="1">MTDRLTQLQLCLDQLIDILFSALSYIDQNHDSVPLNPADPKATDANHNPPTEHEFRSSQQELSTDIILKTRQILTIIDTLPGVGVNKKQQMETIQNLRIELNKTEEEKRQAIMEKENLLDFVNSLIIQVGESIATTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNF1</accession>
    <name>A0A1E3NNF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_27983</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018743.1">
        <property type="nucleotide sequence ID" value="XM_019162526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNF1"/>
    <dbReference type="STRING" id="763406.A0A1E3NNF1"/>
    <dbReference type="GeneID" id="30179213"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036010">
        <property type="term" value="P:protein localization to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="VPS9" evidence="4">
        <location>
            <begin position="107"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="359"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="47359" checksum="F1B6AA595699AEC8" modified="2017-01-18" version="1" fragment="single">FDFQTFLAQFKSKDCQHAHKYLKSFLDQFNQRIWTVEEQIKLLSDFHEFLFEKLTQYKPFDEMGTDEAKINNCKEGLEKLIMTKVYSSVFSPAMSFIKLTDSHKHDKLMDRKYLMNTYLYNWVELRHLDLDMRVKVESNFITLASAELSKIDNYKSPRDKIVCILNSSKIIFGLIRQQETQENADSFVPLLIYVLIHSRFKHLYSNLQYIERFRNRDFLIGETSYYVSTLQIACNFIVNITKEQLTVGDSEYEQKMIDSKIKFKELQQDTRSGEDSPSQVLTKSAEMVKQSLSNSINSFIQNITADDEHGNSIAEQQSAQQPRRRQRNTNHLEVSDQELERIKQLSVEENTALDHQRLRRKEILQQLVSMFPTLDQGLIEDVLGGSLSSDGYNVSECVNSLLALSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNG4</accession>
    <name>A0A1E3NNG4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_58059</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018754.1">
        <property type="nucleotide sequence ID" value="XM_019163274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNG4"/>
    <dbReference type="GeneID" id="30179961"/>
    <dbReference type="OrthoDB" id="1650at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22525">
        <property type="entry name" value="KH-I_Rrp4_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000038">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21266">
        <property type="entry name" value="S1_RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="7">
        <location>
            <begin position="17"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="RRP4 S1" evidence="9">
        <location>
            <begin position="66"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="160"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="321" mass="35413" checksum="DEBE82240B736DFA" modified="2017-01-18" version="1">MTCRHVRIKTRIDNRSIVTPGELITEDATWMRGHGTYYLDECTYSSVAGMISKVNKLLSVVPFKGRYRPETGDHIVGRIIGINNKRWKVELGAEHAGVLMLGAVNLPGGVLRRKSDSDELQMRSILKEGDLLNCEVQSTFSDGSASLHTRSLKYGKLRNGMFVKVNASLIVRTKNHMHDMPGGVSVILGVNGYCWIFKTKNKVEAAGNSNNAGKAESELKAASKGYSIGMGSVSITRLEEESSWEIYSDKNDNISGSIKETISRYRSCLVALNYCEIAIDVKRLVKAYEISLGLGDVNNLVSEDAKRSIGEDLINSEKMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNJ7</accession>
    <name>A0A1E3NNJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_105842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018166.1">
        <property type="nucleotide sequence ID" value="XM_019159692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNJ7"/>
    <dbReference type="GeneID" id="30176379"/>
    <dbReference type="OrthoDB" id="10674885at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="701" mass="74908" checksum="759E4C5AA7FE158B" modified="2017-01-18" version="1">MLPYIYRRGLLGRRQFATAAACLAKTRRGHGGGGKCHGAGGVPLTSLGERRRPFRPVHRTGETAGQAEKELAAALGQAQEQLRWKEYLDAGDGQYRSGAEEEAAISRRVLEKLIGFSEYSKAGALEKDVWTLLKKQVAIYDNMAGPLEDESAGAAAGEARLELPREIRLAVLQCRATKDYANMLRLYDCFFQLLVKGPLLREAERREELYRLNGCDADADAYGPLVALLDELELSRQTFGDADVDVAVEAYEQTLVRRIEAAWYAVGDNAAGGDAASSLRNLRIYHSGFPALVRLLATDRYACVFGARFVDDFCRDLLRSEMGLEHDELCVMVRNLVGRGRFDAATYAGLRREVAGGPGLAVTPALYGLFLEGAVAGGEPALAAAVAADCAADGYVLDRTLLRALLRMYAAAGDGRRVLRTVDLAVGSGVPLFAPDLRAVVESLVRGGRRSVAVDVVKSLMIVSNGCAEWAAAADAAADADGSERELDDPMLDVGLLFSHYGSDTLLVRPAVDDAVVVPVLATCDTLAEYDALWALVTDDPGFVLTAELVKVDLLLRDRFGVFAGGGHAAAGHAALQRCIHYAMARLPLPQIDRLVLDPDFVEVFVKVCDQLFHTGGLDGDDDALAAAYGALAGVAAEGARSRDALVAELAAHVASGDDAGSGGDGDGTPGQTVARLRQHVRRDWCAGRVQHVLTLSGVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNM4</accession>
    <name>A0A1E3NNM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_117894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018805.1">
        <property type="nucleotide sequence ID" value="XM_019159845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNM4"/>
    <dbReference type="GeneID" id="30176532"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="119" mass="13623" checksum="DF90B5CE19DE1B88" modified="2017-01-18" version="1" fragment="single">MCHTKKQEISCPTSSPVFPPSGSRPQSMAIDFPTLLSPLWIILLLPNRKLLSPLQFGIYKGKPRNGVVLLDFTALFCRNNFSQITLKFSSQFPRIYSHYLQIAAKCVTFIQIREFCRMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNU0</accession>
    <name>A0A1E3NNU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71779</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018860.1">
        <property type="nucleotide sequence ID" value="XM_019164003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNU0"/>
    <dbReference type="STRING" id="763406.A0A1E3NNU0"/>
    <dbReference type="GeneID" id="30180690"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="28"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="482" mass="52030" checksum="0E16938F03C2CBFE" modified="2017-01-18" version="1">MSRPSSSSPLLDAGGLRKKHWTPPLVVCVLICCLASIQYGFHMSELNAPAKYIRLSLGISQSQLGFATAIFSIGGLVSSTFASYISTNYGLKPSFIVTSVCYILGSFIESRAGSHSSLLLGRFVSGLGGGLAIVYVPIYVNDVSPVELRGILGSMTQISVNFGILLAQVLAMRWNSVESWRRILSVGWIIAGVNVVLAATSLVESPKWLVIRSKHADEALGTKILGSLRGSTSSAQDVHNEIEIWKVEKQGHLLLLESSPHLKNLGFWCYLTDKNYFNSKTIATFMMIGQQFAGINSVIFYGVDILNKVFPQYAVLANVLISVGNMVITAVSSTFLDRVGRKPLLLLSLLSMLLSLLVLSAGILTDKPLLTVVSIFTYVGSFAIGCGPIPFLIVSEVSQVEVKDIAQSWATDCNWVSVFVVGTMFPILNTLLGGWVYLIFAAVCVLFAVFVHNFVPETKGCATYDEVWAATGTGTGAGDRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP29</accession>
    <name>A0A1E3NP29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase 48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018551.1">
        <property type="nucleotide sequence ID" value="XM_019160802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP29"/>
    <dbReference type="STRING" id="763406.A0A1E3NP29"/>
    <dbReference type="GeneID" id="30177489"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF15">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="685"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="739"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="767"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="856"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="890"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="935"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1093"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 48" evidence="2">
        <location>
            <begin position="95"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1166" mass="134398" checksum="B626898B87539D73" modified="2017-01-18" version="1">MLSPWRNVFTRLPSRIHSKLLATAKLEVKYDSIFLISQIWNALIISLYREHLLSQDQAQKMIYSLVPDETNGGMALRAPTFFLYQDDSGKEITDFFVPNSEAERRISFFAQSLSSSIPEPIPVEAMPQFTVFIPHYSEKILLELKEIIRQDPSSKMSLLEYLKVMFPHEWRNFVADTKIMAMSQTLKDSQNDGSDSGYLTPKTEKEFVDNKINDLPLYCIGYKSSAPEYIMRTRIWASLKTQTLYRTVSGFMNYTKAIKLLHKVENPEMENYFNSPSTFEDYLNLLADRKFRMLVSMQKLQSFDEKEMSNVNAMINTYPSLLIASLKKEIDPETEETKYFSVMYTIEEKVDKEDPKIKANELKQKFKIQLSGNPILGDGKSDNQNMSIIYYRGEFIQVIDANQDNYLEECLKIRSLLSEFENLDDVSEYPYIPSEHYKNKSPVAIVGAREYIFSENSGVLGDVAASKEQTFGTMFARTLSEIGGKLHYGHPDFLNAIFMCTRGGISKAQKGLHLNEDIYAGMNAICRGGKIKHCDYYQCGKGRDLGFSSILNFTTKIGGGMGEQLLSREYYYIGTQMSLDRFLSFYYAHPGFHINNLFIMFSLEVFMLTVINLGALNHELISCIYDKDVPITDLQIPIGCQNLQPVLDWVSRYVLSIFICFFISFVPLVLHELSERGVWKAGNRLFMHFVSLSPIFEVFVCQIYTDSLKKDIIFGGARYISTGRGFSITRVSFTKLYSAYAPTSIYAGSRLFLLLLFATISMWQPSVLWFWITLFSLTLSPFIFNPHQFSWTDFFLDYREFIRWLSRGNSKWHLNSWITYTRLTRSRFTGFKRAKLKNGENEKILEHNPKAPFMNAFMAEVLAPFIQAFFIVVAYLFMNSQSGVENPEEVNLLIRLVIVTICPIVLNTIVLIIVFPLSLFAGPILSLCCTKIPSVLAAISHLFAVFIHIITFEMIWVLEGFNFSRTLILFTSSIFLQRCLIQACKLFFLSREMKEDRSNRAWWSGKWFELGKYAFTQPLREFIVKISEMSLFSGDFFIGHGILISLTPILFVPYIDHWHSCMIMWISPMNRLRGPIYTVSKEKKRKVEAGKYAILYFTIIFFFAAILIAPIVVSKHFEDVVNSMLPEESFGLIQPNHQDNNDTGSRAPHTIMLTKPAEKEFTTFWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP39</accession>
    <name>A0A1E3NP39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Diphthine--ammonia ligase</fullName>
            <ecNumber evidence="1">6.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Diphthamide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diphthamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71889</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphthine-[translation elongation factor 2] + NH4(+) + ATP = diphthamide-[translation elongation factor 2] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10174"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16692"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.14"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.1.14" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018983.1">
        <property type="nucleotide sequence ID" value="XM_019164039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP39"/>
    <dbReference type="STRING" id="763406.A0A1E3NP39"/>
    <dbReference type="GeneID" id="30180726"/>
    <dbReference type="OrthoDB" id="686384at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06155">
        <property type="entry name" value="eu_AANH_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06156">
        <property type="entry name" value="eu_AANH_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000145">
        <property type="entry name" value="ATP-binding domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="634" mass="71836" checksum="AF73209917439F55" modified="2017-01-18" version="1">MKFVALVSGGKDSCFNILHCLAQGHELICLANLYPPSSENDELDSYMYQTVGHDVLTLYEKCTGKPMYREPILGLASNQKLEYSKTANDETEDLFRLLSIVVKHHPDVEAVSVGAILSSYQRTRVEDVCFRLGLTSLAYLWQRDQGELMEEMCNSGMDARIVKVAAIGLDDANLDMTLQQIHPTLIQLNRRFGVHICGEGGEFETSVLDAPFFEFGRIEILEKEVVKHTNDDVWYLKIKVGFVEKPKNLPKIENWSDFIVEPPLLTEQFQEIYDNVSFQENKPCTTENIFPKAEVSSWSENVFAMNDKVFVSNLFSVKESIEEQIAEIFEKLQNILSEHDLKFSNIQSSKLLVKDMANFGKINSMYVKYFNEPLPPARICVETNLPDTIHAQLSVVILKDIKTKSGLHVQGRSYWAPCNIGPYSQGIADSNDSIIRISGQIPLIPKNMELSCHDKALSAVLSLQHYDNVKEVTGYKFHLLTICFIKNMSLVDVASGVFDEYILTSNEREKFTSNLIIVQVTELPRGADVEWGGLFYKDQGQYMNYDSDSDDENSDGGSDKDIRKKVGVYFVDELSDFKFDINKSAFYEIYANPKKLANLPHQLKDCSAYELFPVIQVIDRLTGSGDKIAIVEYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPB2</accession>
    <name>A0A1E3NPB2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_24370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019032.1">
        <property type="nucleotide sequence ID" value="XM_019162348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPB2"/>
    <dbReference type="GeneID" id="30179035"/>
    <dbReference type="OrthoDB" id="978at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012901">
        <property type="entry name" value="CARME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303:SF11">
        <property type="entry name" value="AER338CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07942">
        <property type="entry name" value="CARME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01296">
        <property type="entry name" value="N2227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="347" mass="40187" checksum="66BD56FC966E9AB0" modified="2017-01-18" version="1" fragment="single">DRSRNELVSSVYDINSYLQNAYKWNNRRRVLFRQMSPLQQKIASCIDYPKKLNLVDNHFDKNNEILSTIVSNAIKKYEIKDFELKLVEEQATASKRNNYFRVVEGLCHYARDWPVTPSEEVTPLLEYIKQQCKDLDNDKTVAIVPGSGLGKVSHTLAQLNFSSVHAVEFSWLMVLMNEFLYLKQENIKKLNIYPYLHTYSNHLTVDDQLRPVEISHSIAKPSNLAIHNADFTKFKIQEHLRPTESPENLLFVTCFFLDTAENLIEYLQAINRISSSFKGTKKWINLGPLKYGTAAKIEVSDMELRTLVKGMGWDFADEPDPKLLGYLTDKKGLWQGYYNVTMWSAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPF6</accession>
    <name>A0A1E3NPF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cornichon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_30629</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019113.1">
        <property type="nucleotide sequence ID" value="XM_019162645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPF6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPF6"/>
    <dbReference type="GeneID" id="30179332"/>
    <dbReference type="OrthoDB" id="434393at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033466">
        <property type="entry name" value="Cornichon_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01340">
        <property type="entry name" value="CORNICHON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16248" checksum="DB38AB5B48ACD616" modified="2017-01-18" version="1">MFSEIFLFILAVIVNALCLFTEVYFSISFSDLECDYINPIELCNKLNRYIVPKAAVHGVLSILFLLNGYWFVFLLNLPLLAFNAEKIYRKNHLLDATEIFRTLGKNKKESFIKLAFFLLMFFFYLYRMIAALIADDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPJ1</accession>
    <name>A0A1E3NPJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15883</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019154.1">
        <property type="nucleotide sequence ID" value="XM_019161004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPJ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPJ1"/>
    <dbReference type="GeneID" id="30177691"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="389" mass="44681" checksum="61CDB9E8B59B7944" modified="2017-01-18" version="1">MAPYGQVVIGPPGSGKSTYCNGMNQFLNSIGRNSLIINLDPANDRLPYDCTLDIRDVITLEEIMDDEKLGPNGGLIHCLEIFKESIDFFIDKIRELIKLSMDGQSTYIIFDCPGQTELFTTTPVFKSIFQQLKTVLDFRLCVVSLIDSINLTIPSHWISSLLLTLRSMLQMELPQINVVSKIDLLNGYVQSDKLNKIEQQKKNTTLRSNADSDQKDEDPEGGLPFKLQYYTDVEDLHYLTPYIKSENNQRSHAWFNEKYSKLTESIADLVEDFGLLSFSVLAIEDKTSMINLLSLIDKSNGYCFGTNELGGDSIWTDAVRQSSMGFMEDIDIQERWIDEKETWDALEEEKHKELLEYYQKHEKPFDAETEWEEALKEWEQNNKEETLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPP7</accession>
    <name>A0A1E3NPP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_31991</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019180.1">
        <property type="nucleotide sequence ID" value="XM_019162735.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NPP7"/>
    <dbReference type="GeneID" id="30179422"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="595"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="631"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="656"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1152"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1179"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1238"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1267"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1296"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1327"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1421"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="132"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="810"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1489" mass="168337" checksum="2B8E4FB503062C32" modified="2017-01-18" version="1">MSDSHSIGTDSVDSAGGEVKQFNPIDDVEAQENGENLSQHLSRILSTPEGIQKIESLARVLSTKTKADLDHFEVNPQDFDLKLLLKYLHERASEQGIESAHSGLAFKDLTCWGVDASAAYGPSVPEMLRDSVKWPAKFFKKDTRLQRQIIRDFMGIIKPGELVLSLGKPGAGCSSLLKACAALVEGTFSYDGLDQEEMMKKYKGYIVYNPELDFHFPYITVKETIQFALRTKTPKTRIDSMGREEYVDNMLRLWCTVFGLTHTYSTRVGNDFVRGVSGGERKRVSIVEALSMNASFYCFDNATRGLDASTALEFTQCLRTATNILRCGALVAIYQAGQNIYELFDKVTVIYKGRQIYWGPAKTGVSYFERMGYVKPSRMTAPEFLTAVTSPASRQIKPGFEGKVPDTADEFEQYWFSSPEYQELLAEYDEFVATHNAEETRARLELATSQRKQTGQRNKSIFVVNYFAQLRYLVIRGFQRTKGDITYTMVYLSSFVTKGFVVGSMYYHIPKSTVGAYSRGGMLFYCLLFCALTSLAEIANSFANRPILVKQKSYSMYHLSAEALQEMITELPTKAIAVIILSLTSYWMPNLKHTAGAFFMFFLFLLTIQQCMSFIFKLVATLTKDGTTAHAVGGLWVLMLCVYAGFMLPLPKMHHWIKWIHYLNPMFYCYYSLMANEFHHRIMPCYKLVPSGPGYENVSLINQVCDLPGAVAGNDWVSGEAYILRHFDFRWHNVWKYWGINLCWTAGFIGLNVFMAEFVKNVEGGGDMLLFKRGHMPDAAGGEEWDGKVATREEMMLALNGPDADLEKIISEADIFSWKNLDYIIPYEGATRQLLCSIQGYVKPGTMTALMGESGAGKTTLLNVLAQRISFGTITGDMLVNGRPLDSSFKRRTGYVQQQDLHMAEFSVRESLRFAADLRQPKSTPQAEKYEYVEKIIGCLGMDKYAEAMVGKIGRGLNVEQRKKLSIATELVAKPSLLLFLDEPTSGLDSESSWSIVQFMKALADSGQAILCTIHQPSATLFEVFDRLLLLKKGGKTVYFGDIGPNSSTMLGYFERYSGVNCGVSENPAEYILNCIGAGATASTAQDWAELWNNSPECAQVTADIEQLHAELPQRPINSNVGDLTAKYATTYDKQFWFVLKRTYLQFWRSPVYIRAKFLECVFCALFVGLSYIGFDHTIAGASGAFSSVFMLLLISLAMINQSHVFAIDSRELYEVREALSNTFHWSCLLISHTLIEIYWSVICEFFCYICYYWPAQYSGNAPFAGFFFFIYVLIFPIYFISYGLAILYFSPDVPSASMINSNLFASMLLFCGILNPKRWSPRLWSFMYVASPFTYFVQAFVGSIVHDRVLICAFDEYNIMDPPSGQTCGDFLSTYVDNNGGYLVNPTEDTQCKYCPYTKQEQVVEQYGIHYSEHWRNFGIAWIYICFNFGAMCAGYYIFRVRGFSISGLFNVKKWFEKARVDRHEKDTTIFQKKEGDDKVLVPKKPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPR7</accession>
    <name>A0A1E3NPR7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_26414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019217.1">
        <property type="nucleotide sequence ID" value="XM_019162449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPR7"/>
    <dbReference type="STRING" id="763406.A0A1E3NPR7"/>
    <dbReference type="GeneID" id="30179136"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="83" mass="9305" checksum="E80DCEF38737F1D2" modified="2017-01-18" version="1" fragment="single">MISAIFIINSSGTVIVSRVFREDVKCSVSEVFRSKIINVDPKNLKNPILTLGSTTFLHEKRGEIYFVAVTRNNSDASVILQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPS6</accession>
    <name>A0A1E3NPS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_72096</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019227.1">
        <property type="nucleotide sequence ID" value="XM_019164100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPS6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPS6"/>
    <dbReference type="GeneID" id="30180787"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="179"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="291" mass="33748" checksum="A3EA948A29B66BEA" modified="2017-01-18" version="1">MVNLIINPLSRKLKKINCDSAPNTRLSAIIEAYAKKNSVSDPNRIKFSYLAEENDKERDKKKRLPLKSYLTLEENGLDFAKASTLNVYAKDVGPQIGWRTVYFLENLGPMIIHSLFYYGYYSASDMTYTQVLAYNLTLLHYLKKEYETFFIHTFSADTMPFAFLFRKSGHYWINSLLIAFSVYSPQNVVYSNKFWSVVHHVEDRSLDELKWFGGALVLFEICNLYCHIILRRLRSDGTRAHKIPHGFAFKFVSFPNYLFEFLSWTVFAVLTNNWSSSVGAFKFGLTELGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ10</accession>
    <name>A0A1E3NQ10_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_123434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019239.1">
        <property type="nucleotide sequence ID" value="XM_019159916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ10"/>
    <dbReference type="GeneID" id="30176603"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="158" mass="16938" checksum="BD18FB5234EE9B5B" modified="2017-01-18" version="1">MLGVCYGATCWNTACLLEGRYRGRHAAQRGGESDAHMGTENLTTQDKVHKRCGQYSAASESKRVHLHYNGQAPRGDGPTSRCRRAGEPARPQAECSLLSASSCLLLPPPASSCLLLPLSVDSTSACLFLSRPSPCHHGLLLAGLRLNNLPLCPEELLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ60</accession>
    <name>A0A1E3NQ60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019334.1">
        <property type="nucleotide sequence ID" value="XM_019159758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ60"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ60"/>
    <dbReference type="GeneID" id="30176445"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF31">
        <property type="entry name" value="ACYLGLYCEROL KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="2">
        <location>
            <begin position="178"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="595" mass="66589" checksum="A8D56B55D08AB484" modified="2017-01-18" version="1">MWRNCFHWPQIAFLTTTKSSLCYRESRHMKATIDLEGKRLVLSGQPLMVTTTPQLDNPLACCLPVSTATSSFNESRQVNSLIPFRNILWVEGTSSGKPNEVEVTYVYPIFDEIPIQIPNKVGEFKLPYEDAKRKVYPMSQILTIELEDASVLGDENISLTDFIIRCSYTHSQFSLNGFRQQSIFVLINPHSGTSEATKIFKEEVQPILSAAHCNLEVQYTEYKGHAIDIAKSINIQKYDTILCASGDGIPYEVINGFFQREDRAEAFSKINVVQTPGGSGNAMSLSCLGATSASLAALRILKGRPSQCDLMAVGKESSDEVIVSFLSQTFGAIAQADIGTEWMRSIGSIRFDLGVAYEVLAGHKYPCDLAVKWKCKNKEEVSNYYMNESQTKGTPESRSRRGTIERGGYKDSVNVSTERYSKSSSAFSAAGSDLKELSDDDFELKYQSFFKESKDFSTLPDDWEIYDKGTTNNMRIFYAGKMPYIAATTNFFPAAMPSDGAIDLVVFDSRSRIIPTAGALLSLEKGTHVWEDCVDHSKVEAFRLIPKADRECYISVDGEWFPYGAFQVEVLNKVLRTILWEGDFTETGFLENKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ89</accession>
    <name>A0A1E3NQ89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_76891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018991.1">
        <property type="nucleotide sequence ID" value="XM_019164953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ89"/>
    <dbReference type="GeneID" id="30181640"/>
    <dbReference type="OrthoDB" id="2138282at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133457" evidence="2">
        <location>
            <begin position="20"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="410" mass="47227" checksum="3BE968A8831736DF" modified="2017-01-18" version="1">MRFTTLTAATTTALAVAQAAPINVDDAATKDVSNSKGFLDSLVDSISSLMESNEEAGAADPETPGEHWKHYGKQQKKYWKKIGKQYKKKYTPPADSDDEDEEVEEMEVGEPVETPGEHWKHYGKEQRKHWKNVGKQYKKKYSPPACSDDEEEEVEAIEDGEPVETPGDHWKKIGKDQKKHWKNVGKDQKKKYTPPADSDDEEAELMEVGEPVETPGEHWKHYGKEQRKHWKKVGKQYKKKYTPPTESDDEDDSEMMEVVFMLAEEPVETPGDHWKKIGKDQKKHWKNVGKQYKKKYTPPADSDDEDVELMEAGEPVETPGDHWKKIGKDQKKHWKKIGKDQKKKYTPPADSDDEDVELMEAGEPVETPGEHWKHYGKEQRKHWKKVGKQYKKKYTPPADSDDELDAEALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQE2</accession>
    <name>A0A1E3NQE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3',5'-cyclic-nucleotide phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_50982</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the cyclic nucleotide phosphodiesterase class-II family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019041.1">
        <property type="nucleotide sequence ID" value="XM_019163149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQE2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQE2"/>
    <dbReference type="GeneID" id="30179836"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07735">
        <property type="entry name" value="class_II_PDE_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024225">
        <property type="entry name" value="cAMP-PdiesteraseII_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000396">
        <property type="entry name" value="Pdiesterase2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28283">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28283:SF1">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02112">
        <property type="entry name" value="PDEase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000962">
        <property type="entry name" value="Cyc_nuc_PDEase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00388">
        <property type="entry name" value="PDIESTERASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00607">
        <property type="entry name" value="PDEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0114">cAMP</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="40843" checksum="FB86A3CF5095978D" modified="2017-01-18" version="1">MPTNRGVFELTILGCSGGPISGKTCSFMLKPTDVDYAAIVSQDGAAEGALLVIDAGTGLTSLVDILQNSSSYMESCFLLELYPQNQDLIATYVNTQNLAITAPLRALRTAAAGTRSSLQLADDVLNSIDAYLITHPHLDHVASLVINSPAFSSPKAVYGLPDTVASIKDNLFNGRVWPDLVSMGIIALHQLADSVRVTDISRRYSITPFKVSHGITASGSRYISTAFLITDTKHDSSILLFGDVESDVVANCNYNKVIWTHAAPLITANKLNTIVIECSTVDKPPPLFGHMTPFNLIYELSTLRKLCVDLRYPDTYIGSRYQPLAGFNVIIIHVKETLDDVNPRCLILQRLNTLNTNFNLKINFTMALSGLSFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQN7</accession>
    <name>A0A1E3NQN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_8945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019514.1">
        <property type="nucleotide sequence ID" value="XM_019165046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQN7"/>
    <dbReference type="GeneID" id="30181733"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="239" mass="25501" checksum="66C8D42C02B1B424" modified="2017-01-18" version="1">MVWFNLVLSNSDGEESTSGSGTDSSPTSTTTVTRTLTSTTTRYYDMVVTLEKRATATPAADHMAVTSNTSSASSSALDHRLVAAIVIPLATIAIVCVSMLLSRHLRHKRNREQQRLQHTNKNEKACVQTVPAVYRHPRHPHQPPPTLAPATLNPPPPPPPATPLALAFQLLFAPSPSTATSFSSLASSPVNSPVSSPATVHAAVAAAWAYKPLFSLPTAAELDCKKTAASNQFSHHQHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQV3</accession>
    <name>A0A1E3NQV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell division control protein 42 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_70068</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in hyphal formation, virulence, morphogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019545.1">
        <property type="nucleotide sequence ID" value="XM_019163538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQV3"/>
    <dbReference type="GeneID" id="30180225"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007119">
        <property type="term" value="P:budding cell isotropic bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000222">
        <property type="term" value="P:positive regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022604">
        <property type="term" value="P:regulation of cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060178">
        <property type="term" value="P:regulation of exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01874">
        <property type="entry name" value="Cdc42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000236">
        <property type="entry name" value="Cell division control protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037874">
        <property type="entry name" value="Cdc42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="195" mass="21673" checksum="AC882461ABCC0791" modified="2017-01-18" version="1">MGSFNNTIKCVVVGDGAVGKTCLLISYTSNQFPEDYIPTVFDNYAVTVMIGDEPYTFGLFDTAGQEDYDRLRPLSYPQTDIFLICFSVVVPSSFDNVKEKWFPEILHHAPHVPCIIVGTQIDLRDDQTALANLARQHQKPISAQQGERLARELKAVKYDECSALTQVGLKSLFDDAIVAALEPPVIKKSKKCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQV8</accession>
    <name>A0A1E3NQV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70112</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019592.1">
        <property type="nucleotide sequence ID" value="XM_019163557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQV8"/>
    <dbReference type="GeneID" id="30180244"/>
    <dbReference type="OrthoDB" id="605656at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902906">
        <property type="term" value="P:proteasome storage granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056263">
        <property type="entry name" value="RPN11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23594">
        <property type="entry name" value="RPN11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="35"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="313" mass="34846" checksum="9C1B7A48F912E8C7" modified="2017-01-18" version="1">MERLQSLLGGRMGGMGGMGGPGFQDSPAIDNAETVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDFTITVVDVFAMPQSGTGVSVEAVDDVFQTKMMDMLKQTGRDQMVVGWYHSHPGFGCWLSSVDVNTQQSFEQLNPRSVAVVIDPIQSVKGKVVIDAFRSIPSQVLMMGQEPRQTTSNVGLLNKPTIQALLHGLNRSYYSLNIDYRKTEKETDMLLNLHKKEWTSGLKLKNYDDQDCENLEGSKKLIKVAKQYVERIQEEKTLTEDQLKTRYVGKQDPKKHLTATAENLIEDNNVSIVTSNVNRLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR84</accession>
    <name>A0A1E3NR84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70212</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019697.1">
        <property type="nucleotide sequence ID" value="XM_019163590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR84"/>
    <dbReference type="STRING" id="763406.A0A1E3NR84"/>
    <dbReference type="GeneID" id="30180277"/>
    <dbReference type="OrthoDB" id="992776at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100">
        <property type="entry name" value="IMP2'P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100:SF4">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="283"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="465" mass="51649" checksum="9DF8363B242CCE89" modified="2017-01-18" version="1">MSLEGAIGEEKKLMSLLRERQYKSENAPRSRSASRNPKECSVATRSESVTPSVYEEQVYPESITFKDPSSRLSTKPIADDQIDVSSEERLSDSDYEFSVNDEGRILPNYSSSGPVSSSKKKVDELTLLNERKMMKKAESAKIADSLLASERAISNAKAIGRNIPTEVINTLEEQAKDSGTEIHVDPEEFYQERAARKNKLEEYAVYKKKLISSGTTSNAEFIAPYTRDQEDKIDTELATVLNSKTQISDIDSDVSNQRAVRTITRGNFFETLISSDVKHPKSFVLCMDFSDESKYALEWCVGTVLVDSSVLYILNVLEDDDYSSMNLNGIQPTSSGKSLATDHPSGTTRERTRVNNVNEITKLVVDLLKLTKLQIHVVIESCHHPIPRHFIVGVIKHMSPNLVVVGSKGSSAIKGVLMGSLSNHLVRKSPVPVMVVRNRLKKIAKKSKFSNNVTSLKGLADARID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRB0</accession>
    <name>A0A1E3NRB0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_84575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019735.1">
        <property type="nucleotide sequence ID" value="XM_019165009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRB0"/>
    <dbReference type="GeneID" id="30181696"/>
    <dbReference type="OrthoDB" id="3998270at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="678"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="802" mass="87904" checksum="D54460FC66303C18" modified="2017-01-18" version="1">MANTHISKLSYLGDPLGFEFPSSEESLLPNDEEQEKIERAGSIFIKSGMKKVSPFPTVSEGSERTTGKHIFAAIITGKGQADPANNSKTEEMEENGIEDNKSDAIVDKTFEKESSEKTRSNTEESHYEDQRTFLPQNSLIRKRASLRQVNSISSFVNPYLRDSLLRKHQKEIANDENLSKRVQSMELGVRASPEVADLAELEEEDVESEIDKDKLEIPRRSSKRITRLQSIKKWSQLTRNSSMAHRNRNLSRSNTKKSVNTLANDPKRYRHSFKSVDLSGLDQILVELMNVKEGSAINTRQNSTHSKTSIRNAHENLVKDESSEVNVKPFNVSKSISRNRSLKRSITTAKNEMKTISEHSYASSEPSRTHHQETGLPGGKESVKVDSGCELKRSNAVKRKPVLIEYLLKILRAFKEAGKKCWRVIKSPTRKNVAFKGKKAPGTPKRGHKKKMTISEPVLVDIADTPFGSQVINMGDAGPASAASAAASPSSSAPSAAVTSGSSKSAAAAVPVTERLSTIASMGEDEKSKHFSVEDGAARGEPDRLEELWRQYLSATIRNRIDMKLDVSKLDYIERVKSIQSHPSRRESIERSGNVQVEKILDRYIVSAESSRASTITASVSSVADSMVTAPLYRSSSEAASSHTRVSTRTWATIDSDSDAASTSSVSTIEEESDFDGLRSGRSSVSSLDPTQPSEDVSDVYDDYSMLSDADDPAARHSDAGVATATGTVASSYTLGHKPSARTLKPSARILNLQGVLTRRNSAVSRTRLGGSVYSTSVCSAPDTDRSDAFSFTRSVPSVCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRP0</accession>
    <name>A0A1E3NRP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72197</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019336.1">
        <property type="nucleotide sequence ID" value="XM_019164121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRP0"/>
    <dbReference type="GeneID" id="30180808"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="25"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="174" mass="19179" checksum="BB8B3138CB632CBE" modified="2017-01-18" version="1">MHVRWTDRALNSVFCHYDNKNSLTRDELANERSFLAYIRCTTTLLVSSFSITQVVLQMIILSVRRITAVDGVAGGGGGSSSSSDTLPLLIAAYASNIHDYTHYFKPTTLLICAVAGFCSCLGLKKAWLNFYNLAERAKFQPGLIGMGLLFLSVFVVDVVLLRQSIAFGLREFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRQ1</accession>
    <name>A0A1E3NRQ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019910.1">
        <property type="nucleotide sequence ID" value="XM_019160328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRQ1"/>
    <dbReference type="GeneID" id="30177015"/>
    <dbReference type="OrthoDB" id="3993280at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="148" mass="16899" checksum="259F3F8D5D2457AD" modified="2017-01-18" version="1">MKEKQTKELERHERARAHQARREKAQKYLGYSDFLNSSECDSNGYRFDETDDFHKLDEAGWNRVVGFVLVRIVSPLILVVQGIVVGLVLWRVYDLDFSPTGVLAQQNCLPWVAGALSLNAFLVHGLSKHVFFCKSYYKPRASHGPSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRS7</accession>
    <name>A0A1E3NRS7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_48108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019918.1">
        <property type="nucleotide sequence ID" value="XM_019163134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRS7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRS7"/>
    <dbReference type="GeneID" id="30179821"/>
    <dbReference type="OrthoDB" id="60843at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904775">
        <property type="term" value="P:positive regulation of ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="67"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="336" mass="37511" checksum="BC893112AC2922B8" modified="2017-01-18" version="1">MNQRNSCFSNFGSFRKFSSSTEDAFRRFRSFANGEQFTKRFVDSSLQYTVSVAYSPKDRLKNNMSDDDALNAKNVYESPTGEDNYILAYTEIGIIIGVLDGVGGWSEQGFDSSAISRELANKVTIFYLENPSSSPLEILQKSFAAVKEEGTVKVGSTTICFGIIDGRTGKLSALNLGDSWFGIFRKDENSRYKCTHQSVEQVYYFNAPYQLSIIPDDFLEIAKKRGSKYLMNEPDESEEYSYELKSGDIIVFTTDGMVDNVFPDDIEIYLNDNASEGVNLSESLGSLNKILVQQTTVLSLNTNFKSAFSQKLSSLTGQDYIGGKPDDITSVMVYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV8</accession>
    <name>A0A1E3NRV8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_125842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019406.1">
        <property type="nucleotide sequence ID" value="XM_019159948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV8"/>
    <dbReference type="GeneID" id="30176635"/>
    <dbReference type="OrthoDB" id="1865897at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="981" mass="113308" checksum="5680CC4E4677A681" modified="2017-01-18" version="1">MRSMSFVHRSFFKIKNTPTAFARHFQNRPNLFLPCQYFQGTNLRNFSSFTKSDDRIFTPDGFSSTGLSSIYTQNLKILDTKIQGENFTNTVHHFESISHNKVDLLPMSGSLVHLKTTDDIESSLSNSFGIVLNDLNFSLSKVSTYRVLLPNGEITEINADHILFTIPMFIRREMMKAMLIDESVLPVEHLLHLTYTLNVFLLFFTKMVNVILKKDLIRTLYLKSAFMNYQSSLNLTLFSIGMYDYSKSLRNTLDIKTNPMGSHVLLLSCHFLIYNDPIHFRYIKSKNSIFNLLNPMNQVTTKYFYNPILLSQNLENIYAQPIDLIVRSYFGILKKSNEFQIYNILKTDENFKRLILMVKYSIVYPDSKLVNKLKALLPIDVSTALSPKVLFEFLVKIGIYDKDTNALISSGFYGLNMYDPTDLSLPVKNVNELQYAYHKGQSFHELPKSLQRMKPLKPVGDEKKEGGKFANRSYSNSFLDVIQDSKLKIWPKSLVRNRRTVYKLTKTIAFSVSQESLTNYRFNIFIPIPNQSPNENITVQEPIQIDNNVVTFPKLEKFNHALRINQPCIRLTFNHNLIDSDSLTSPNIKVGLDVFKKIENIDEEWFKNRNPSSLKGSKKKLDCWLSLNKLMNLLIEKEKSRVRLGYLKTFGDSDESNRNFLNFKTFFIDNKSNEDGSDIKLENVSNTNSAADTHNSTTVGGGNLLNENENYMLRDKRWITENLKLLIDENLSRFCSEKGVNVANRSVAKRILNSVDYRVFKKFKIFKWYANSYETFNFQLSSNPGDLTAYVSCLAFLGEGSYFFNNPKGTAKNITNENGYTPLGLKHFSSFTSLNYIETHLNLWQLFRYLAIESFELVEDRNKQNWDPLKLDKVTNSHYNKCLSQTEGYLEFINRLNRFEVLRNIQKDVSGGTNIHGYMLMRCVVTSITQERIIGYWFEKDIEVEIIVDSSSSKSKTMIGDRLLCSEVVSVNPLEDVIVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS90</accession>
    <name>A0A1E3NS90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WDR5-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_14501</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020108.1">
        <property type="nucleotide sequence ID" value="XM_019160405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS90"/>
    <dbReference type="STRING" id="763406.A0A1E3NS90"/>
    <dbReference type="GeneID" id="30177092"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="4">
        <location>
            <begin position="16"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="99"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="317" mass="35871" checksum="F092AEC4095DB207" modified="2017-01-18" version="1">MDNNSLKYKLRYRIPKTHQHLVSTVEISPDGHTIATCSADKTVKLWEAQTGKLLATLRGHNEGISDIAFSFNNKYLASASDDLTIRIWDLKYEKTIKLLKNHTYHVNCLKFDKRSRILISGSSDENIRVWDCKRGRSMRTLSAHSDPISSLDLSFDDTIIVSGSYDGLIRLFDTETGGCLKTLIYDKEGSSFPISHVCFSPNSKYILSSSLDGVLRLWDYMNNKVLKTYRNVDGLPIAEKYSLGTNFIVYNNESMINSGDEQGNILFWDIQSQELLFNLKASECDSPIMQVKSHHSGKSLVSVSLEGELCVWDYVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSB6</accession>
    <name>A0A1E3NSB6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="3 14">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_57103</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
            <location evidence="1 14">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="3 14"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020075.1">
        <property type="nucleotide sequence ID" value="XM_019163213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSB6"/>
    <dbReference type="STRING" id="763406.A0A1E3NSB6"/>
    <dbReference type="GeneID" id="30179900"/>
    <dbReference type="OrthoDB" id="18042at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052723">
        <property type="term" value="F:inositol hexakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052724">
        <property type="term" value="F:inositol hexakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000830">
        <property type="term" value="F:inositol hexakisphosphate 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000831">
        <property type="term" value="F:inositol hexakisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052846">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052843">
        <property type="term" value="F:inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071545">
        <property type="term" value="P:inositol phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051516">
        <property type="term" value="P:regulation of bipolar cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110162">
        <property type="term" value="P:regulation of mitotic spindle elongation (spindle phase three)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000036">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000002">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF9">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="17">
        <location>
            <begin position="193"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp fold RimK-type" evidence="16">
        <location>
            <begin position="399"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="83"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ48962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1127" mass="127851" checksum="63BD862129CB0A18" modified="2017-01-18" version="1">MSIDSAVDDDRLLEEQERYDADPVLLTKESLDYHHRAIASIAPILEDFTPVLSKSEIVSPSQDALSRTVSNVSALSISHHQPSVALSSSGSNNNSPSDTIVIEDSKKEPEIQDSELSQSDKSKSSEVSGLSINASASNANAAANPVVTSGEASPRPISSSDSFNSILKSRTLSTSSTTDSPSSPKLKLPRIGTIGVCAMDNKVLSKPCRQILQRLIANDEFDTVIFGDKVILDEAVENWPTCDFLMSFFSTGFPLEKAIAYAKLRKPYCLNDLIMQKALWDRRLCLEILKAAKVPSPYRLVISRDGGPQVDDDLRAKLEAIGIDCSEVDEPDWKMVDDDTLMVDGQIIKKPFVEKPVDGEDHNVNIYYSKKNGGGGRRLFRKIGNKSSEFDPQLNEIRTNGSYIYEEFIDTDNFEDVKAYTVGVGYCHAETRKSPVVDGIVRRNTQGKEVRYPTELSEEEVRMAQRVCEYFQQTICGFDLLRTNGKSFVIDVNGFSFVKDNKEYYDTCASILRTKFLEAKTLREQSKVPNPILEKKQKWIFKGFVSVIRHADRTPKQKFKYSFRSPIFISLMKGYKEEIIIREVRDLNVVLQTIKVAREKKLEDEKKLKQLEGALEKKMTFPGTKIQLKPALNKETDEIEKVQLILKWGGEPTHSARYQATDVGEQFRQDIQLLNKAALDDVRVFTSSERRVVASAQLFSKSFLAEEELPDDFLKVRKDLLDDSNAAKDLMDKVKKKLKPLLRQGKEAPPQFAWPPKMPEPFVVIKRVVELMNYHHKMMELNFKTQDVEKFQADWCCGEDPFLFKERWDKLFEEFVSVEKVHPSKISELYDTMKYDALHNRAFLQKIFVRNDDFGNLEEDQSYLSELSGSLVKQYPINILAMNDFKVNDSALDATSTSSAEASSNPAGSIGWVLSSSYKPAHKSQTQRKDSPFDDPKYNLLRELYRLAKVLFDFICPQEYGIDDNEKLDIGLLTSLPLAKQILSDITEIKETNRAGTRVYFTKESHIYTLLNILYESNVPMKIARNALPELDYLSQIVFELYESENTSGSKKHSIRLSLSPGCHTQDPLDVELDDKHYISCIRRINLTRHLDMDLVQQKLKSRFSRVSLPKKFTPVNISSPLSEGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSE4</accession>
    <name>A0A1E3NSE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020039.1">
        <property type="nucleotide sequence ID" value="XM_019160378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSE4"/>
    <dbReference type="GeneID" id="30177065"/>
    <dbReference type="OrthoDB" id="4078670at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="237" mass="27334" checksum="9960DAE7845E462B" modified="2017-01-18" version="1">MIDLKTVPRLGKLSCESTQLLAKVDDYALDYNIFLFEGIGNVIDTNDAQLVSFIESLFITIPKEYSSLIYNPLSLPGKKINPSTTSKLQKRLLSLLIQERHKRDIQLLNEKFDNKIEYVELENPDKYEIKSIYYKTFNSAKDEYADQFGRLALLQSLEYDFEHALDDESDYQNDDDLVEHFCDDRMLDFKLDTSKTENEVMNSELIRVLLPLASQVIMGQEGEGEAEDDETDGEEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSE6</accession>
    <name>A0A1E3NSE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Synaptobrevin homolog YKT6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_30099</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020190.1">
        <property type="nucleotide sequence ID" value="XM_019162628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSE6"/>
    <dbReference type="STRING" id="763406.A0A1E3NSE6"/>
    <dbReference type="GeneID" id="30179315"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Longin" evidence="10">
        <location>
            <begin position="7"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="155"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="215" mass="23688" checksum="E4C3A1781B0ECF67" modified="2017-01-18" version="1">MKIYYIGVVKPAGDDGSKTACICEEKDLSSFSFFQRSSVGQFMTFFAQTIAERTKPDQRQSIEEGEYVGHVYVRPEGVAGVLITDKAYPIRPAYTLLNKVCEDFLSQHSLAHTTQLAAAAAAAAQRGEPAPSGASVFPELAGYLAKYQNPHEADALMKVQQELDETKIILQQSIENVLQRGEKLDALVDKSEALTYSSKTFYKQAKKTNSCCVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSF8</accession>
    <name>A0A1E3NSF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020144.1">
        <property type="nucleotide sequence ID" value="XM_019163687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSF8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSF8"/>
    <dbReference type="GeneID" id="30180374"/>
    <dbReference type="OrthoDB" id="72772at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF5">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="780" mass="88033" checksum="F62339198D791317" modified="2017-01-18" version="1">MTSVPYLQGGGLRSAEGSAIKSDQFLALQLRSSLPSKLLEIRHIKSILIKRITINSEDLNKLSGDTFGSDETRLMKKGGNDLKNNKQNEMDVKVLNDNNKLINSADDLAHDRREKLLRKGKSSYDLSKDFKSGPSLNNVSNRPNTAKDDNYLTTGTSVRNTTLSQPIEIRRQLEFQKINEDTFFDCFYTINNFLNIDDPYFISRVFPSHVELADELEIHAFAKSATINLFVKSEYSSWKLSRQYHIKLSLLVNLGDDLYRVESKLKHLKNLLLLRFTDDCYYTLPNEAISPELIKSLRSTYLQNIIEKFEDFHYKPSSCSYDQIMTMNNYSRCIHDLLHTKLALDDRISQELIKENSLHKILDNQNTLKTSSEHLNDVVHKKVSHNFQLEKKVALIKKQINELKSRVLQLKYPAEVRTSDTSIGLATETSTIQEENSKLTSQINIEKARIASIIQFIFPMKPISNKHDFSLFQTCFPSSLIPQTKSFEKSDHSTIIPISIVSSTIIQRLAQVSRPHSERLNSLIGYIALIVLTVADIFNIPLRYPIRELGSSSYIHDPISNFNVSPRASLPKVLNDPSAAKSATIYPLFICRNATLAVKFTYALLLLRKDLEQLYEVEGIVKVEEFNLLVACKIWLTCVEGYTDNNVDDFEDIDNTSGGSIAGDSTTATNEYDSSNYSSNAISQVYDAFHDDGKVPSLLTASFSRIEPKRRISGCSRFSKFSNASVVSSSSRDLAVSGISAISVGSNASSIVNEYNQKLHTEERIKHIKKHLLKGSSGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSI6</accession>
    <name>A0A1E3NSI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020174.1">
        <property type="nucleotide sequence ID" value="XM_019160425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSI6"/>
    <dbReference type="STRING" id="763406.A0A1E3NSI6"/>
    <dbReference type="GeneID" id="30177112"/>
    <dbReference type="OrthoDB" id="3997048at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF174">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="284"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="844"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="920"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="948"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1039"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="562"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="654"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="721"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="765"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="791"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="853"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="924"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="951"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1045"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1076" mass="118207" checksum="778B6B2E3D97701B" modified="2017-01-18" version="1">MHDLLNSPLFPLEELSPETFVKLPKTPHAESKSVKLYIILAEPHLFLEGFTKDEVLSRPPSILRGCLFLRILHPVKIKEINLKLKGVSRTEWPEGIPPKKTEHFESKGILNHTWPFFSYLNAYPQTANSRNNADLFIPKSDSDVSNFSLDANNNTLSPVTSSTDLKPVLSPVSLLKSFRNTSNTLSSITSSSELTTSLSSSGNDDNKLFPPGDYVYSFELPIQSSLPESVCVTFGSIAYNLEAHVERSGAFRSSLTARRPLSLVRAPFENSSEENEPIIIDRDWENRLQYDIVIYSKQVILNSYLPISFRMIPLEKIKVHRLRVYITEHLEYYCHNKKVHRTEPPKKILLLEHKPPKDCDNLLSLGNDEIGGVELDFQVFIPEYYNEKFHLHPDTTSEDVQSHHWIKISIRISKPEPTPEDPNKRKQYELSIDSPMHILSSHCVHANTLLPSYDEQIKLDRLNSNINHTTNSLTETNTNKTLTPVSSSRQSLDKMMSPRDDTILDSNLFKPDDSVPVEMLSPQAKPFSPIASPQLNAISPELREAPLIRPMELSPIVSPSFRSRSASRASLARNRNLSPTPRHTASVRSVTSLNEPPPPPFVENPPSYAEATKPSKNKKRSSSKRRETTNSSVSMDSSTSGNSGNSALSFSAGKSANNSASNTTSSNNNNTKNSTSSVPTRISLNLGASMGSIPGLNNNGSNHQLHRLGSGLIPISQLNPSGTGTTASSTPSESTPSISAFLDSPLSHPDNSSKYNPQIPPPSLKSPQETPPRSHSPLPSQPINQMGDAPSTVTTLDSSPTRGRKKETQDASSYDLAQDTDLRFKITPIRSASGSPYMRAQQYPSTSIHERTASPMRSASPYRSASPAPIVATDRNLSKLVNSTFMPGFALDDHAPGDSNPEFLTAPQISITGSQGNELEINASSQPSSQRQSADLSSVDQPLLREDDANFSDSSTEEYGNQTGLSDTYNMHANTLGSLNSLDMMLNSNRSQNTLNAFGDSTARVGGVPSKGVPLNYLGNNMDGFDDGTDITSMGRFAEERHGGTSTSTSSSMPISSLSSHWSKLRNPKLTHPDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSJ5</accession>
    <name>A0A1E3NSJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_30024</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020218.1">
        <property type="nucleotide sequence ID" value="XM_019162622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSJ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSJ5"/>
    <dbReference type="GeneID" id="30179309"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990333">
        <property type="term" value="C:mitotic checkpoint complex, CDC20-MAD2 subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040020">
        <property type="term" value="P:regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="9">
        <location>
            <begin position="217"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="343"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="477"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="538" mass="59185" checksum="61E72A527865AC97" modified="2017-01-18" version="1">MTSSSPVKKTPMKTPKYHRTVFGNMSPNLVNQSTNSIFTGSKGVLSPNFHVVNSDWNSTFAAKPSSTSSQAANSDSNKVSKIKSNKSSSNLGSSRKIRRTNTNEFLDRFIPSRQTTSGKLTIEKTKSLPSFAMPSDHIESQTSEIYQNTVAEACGLEVGQRILQFQPVPPESKNSLRRVASNNSNTMKCKFKTRQMISTFAAQARMKKIPSCPEKVLDAPGLVDDFYLNLISWSEDNVLAIALGSSVYCWNANFGSVDLVAECDSIVTSIRWSPDDYYLSIGLDNGNIEIWDIEANSKLRTMSCSNSRIAVQDWSEHILTSGSRMGQILNNDVRVSDHITSKLEYHTAEVCGLQWRPDGLQLASGGNDNVVNIWDCRNSVPVFTKTAHNAAVKAISWCPTQLSLLATGGGSACKKIHFWNSNTGCRINTIETDSQVSSLQWGYSNGIGKEIVSTHGFPNNQISVYSYPSLQKTGVIVDAHESRILNSQLSPNGTILATIAADENLKFWKLFDEQPQAPKDVFNTSTTGKEMGKVMTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSM4</accession>
    <name>A0A1E3NSM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcium-binding protein NCS-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70702</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020248.1">
        <property type="nucleotide sequence ID" value="XM_019163712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSM4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSM4"/>
    <dbReference type="GeneID" id="30180399"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="195" mass="22798" checksum="18CF28CDE56B26DF" modified="2017-01-18" version="1">MGQKPSKLNSEEISTLRQETKFSTRELHQWYKGFKRDVPNGGLTKEEFIKIHKQFYPFGDPTEFAAYAFEAFDINDKRKIDFQDFIKALSIASRGSVEEKLRWAFKMYDRDKDGYISYSDLLIVVRSVYKMVGTKVVKFEESEATPEMKADKIWVGYHKKLSDRAFGKISIQEFSDITNVSSKVIQWVNVYNDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSX2</accession>
    <name>A0A1E3NSX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_9681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020314.1">
        <property type="nucleotide sequence ID" value="XM_019165120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSX2"/>
    <dbReference type="GeneID" id="30181807"/>
    <dbReference type="OrthoDB" id="3990816at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="712" mass="80893" checksum="1B86FEBE0E5D21D2" modified="2017-01-18" version="1">MPYLELGRKSETFSESAPLGQSLVFEHKFPGLAEAPSPINDQKDDGAIVLSVDHFSGPPKTLADTDPSVLKSSSNEDVVMVVPTALNPNKEVTIGIIFLVVSLVFLLIFAFFVYVGYVQYKALLSTKGNDLMDLEGSAETIVVEKPRMNGKIDYKNLNFSTNSKEHQFRESGGSKASEPKPSLMSKSVIVGKLPEPQGSHLKQTYFKPPKLTSRLNKIGEYSEDSLLNDLREKHEIDTSMGHDTPYSFGTPKQRKAFKPPFGNGNKSENSMEKRIERKSERRPDRAPKMALEKKAEKKILSSHVSQMLNKYRKESQGLGAFINDVEKADPGSTFHRERIEKHKPNPQKKITYSIKELVKLEPVDVYQMFEYSYDDGDLLVTDLIFPSIPVLKNNLSMNEIEKILTEIRIGLLSRKTDSVTKVIELFIFITFSFGIKTYANPKVFTLPYGILVDTMIQFDLVSQIPEQLSLVMQSGFVETIILYCINCWKFDKINDGRIQQFFEVWNTKPYIPTKLKLYKFIVTLLLQGYRSSLLKEVLIFFSIPPSPHLLQNIITEALKNETPPDIHDLLQYLRRQLQEEHYNCCSNTEKWRHWEFTTAGMKNSPSVNEQFESSMYKIPGNKYESLTNVNFSNEENIKRQPQKTRNALSDNKCIEALNNANSFQVSKENKLFIGENISKLSLQPESIKMYSSSGELPGSLIASPIHSSPKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT45</accession>
    <name>A0A1E3NT45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinone biosynthesis O-methyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinol 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone-6 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Hexaprenyldihydroxybenzoate methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Polyprenyldihydroxybenzoate methyltransferase</fullName>
            <shortName evidence="5">DHHB methyltransferase</shortName>
            <shortName evidence="5">DHHB-MT</shortName>
            <shortName evidence="5">DHHB-MTase</shortName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
            <ecNumber evidence="5">2.1.1.114</ecNumber>
            <ecNumber evidence="5">2.1.1.64</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COQ3</name>
        <name evidence="7" type="ORF">PICMEDRAFT_66491</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3-demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="2.1.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:81215"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EC" id="2.1.1.114" evidence="5"/>
    <dbReference type="EC" id="2.1.1.64" evidence="5"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020355.1">
        <property type="nucleotide sequence ID" value="XM_019163491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT45"/>
    <dbReference type="STRING" id="763406.A0A1E3NT45"/>
    <dbReference type="GeneID" id="30180178"/>
    <dbReference type="OrthoDB" id="3265906at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061542">
        <property type="term" value="F:3-demethylubiquinol 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120537">
        <property type="term" value="F:3-demethylubiquinone 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010420">
        <property type="term" value="F:polyprenyldihydroxybenzoate methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="6">
        <location>
            <begin position="114"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="303" mass="34619" checksum="7247C10305B2FC75" modified="2017-01-18" version="1">MSRRFSTTCLLSNLKARDAAGAKEHFQQLASTWWDSYGPQRILHKMNVLRMDFIGDNIRSHILVNKNAKSEEEKIFIPGWNYKNLLPNEVSNQIDNELNNATWRIYKDMKLNCLDIGCGGGLLSESLARLPNVRSVKGIDMTPEVISVAREHAKLDPILESKLEYQVTSLDDVPNSETFDVVTCMEMLEHVEYPSVVLKEALSHVKPGGYLFLSTINRDFVSWFTTIFMGEHILKIVPLGTHTYSKYIKEAEIEDFMTTIKDFEVIDSKGCAYLPAVGWFYTPTPNIGNYLMALRRKKDPEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTE5</accession>
    <name>A0A1E3NTE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import receptor subunit TOM20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_48975</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Tom20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020437.1">
        <property type="nucleotide sequence ID" value="XM_019163138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTE5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTE5"/>
    <dbReference type="GeneID" id="30179825"/>
    <dbReference type="OrthoDB" id="2154253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037707">
        <property type="entry name" value="MAS20_rcpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18604" checksum="A0AB65B66FAD2DFB" modified="2017-01-18" version="1">MANPASVTFLSLAAGALGYCLYFDYQRRHNVEFRKSIYHKKRQAEKAARLQNEASKVEKTILLKKKLTESLIAHPLTTENKEQSFIEEVTAAEKLASLPDREIDAAIHFYRALSVYPEPTAILEIYQKSVPAEIYELIMLLTAIYPPANITSLLSESNGSILETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTJ3</accession>
    <name>A0A1E3NTJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_101036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020525.1">
        <property type="nucleotide sequence ID" value="XM_019159630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTJ3"/>
    <dbReference type="GeneID" id="30176317"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="247" mass="26413" checksum="69466BECAF9F3ED6" modified="2017-01-18" version="1">MGSTAPPRRRSVADMEKKQVLSGKSQQVKYSNSEADTHGGDALSAWRRLHRSYVDGTPGRLDAGEPARQRRRPRWTDTKDGKSSSKYVLRHRGQRRNSCSTLARRDWLRLVAPGPIVLSGLFLGQAPHLPTLLGSIHPACDPITKFPCFSPICFPLWANRLPDSSAGGASFSGAETALLPICVGHNARDEGKATGQQPAGRKKGRLPAGGRQEGLERASGGQATATSREIISSGNATATANATPLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTX0</accession>
    <name>A0A1E3NTX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_28423</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020647.1">
        <property type="nucleotide sequence ID" value="XM_019162541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTX0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTX0"/>
    <dbReference type="GeneID" id="30179228"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="37750" checksum="08E7D929537570F5" modified="2017-01-18" version="1">MSSFDPNLLPYFNPSVERRVCLITGGNSGLGYYTVLHLYLHGYVVYLAGRSQQRVQNAIELLKKEAAARRTKYTHAQLGKRFLGSLHLLQIDLLSLDSVEQAAKEFRQKEKCLHLLVLNAGIMAVPYGTTVDRFEVQLQTSYVSHFLLTDRLLGLMTSVESDEQDPRIIFVSSVGHWFAPFHFSLDYQFDYFPNIIFTLFRYGMAKCAGIHFVKSLARRHPSVLAVAVHPGIVLNTNLFSHLTHLPLIGTLFWVFFQIFDWLLGVSNEEGSYASVRCALSDGVDRPADNGKYYTTFGLEMTPSPVANNTVYADETWAWTVRQLDDRGHTIETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU28</accession>
    <name>A0A1E3NU28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flap endonuclease 1</fullName>
            <shortName evidence="15">FEN-1</shortName>
            <ecNumber evidence="15">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_71042</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleoplasm</location>
        </subcellularLocation>
        <text evidence="15">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020609.1">
        <property type="nucleotide sequence ID" value="XM_019163807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU28"/>
    <dbReference type="STRING" id="763406.A0A1E3NU28"/>
    <dbReference type="GeneID" id="30180494"/>
    <dbReference type="OrthoDB" id="1937206at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007534">
        <property type="term" value="P:gene conversion at mating-type locus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09907">
        <property type="entry name" value="H3TH_FEN1-Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000003">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="16">
        <location>
            <begin position="147"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ49496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="42570" checksum="BC6B321707DFBAA4" modified="2017-01-18" version="1">MGIKGLNALVKENAPGAIIASEMKSLFGRKIAIDASMCLYQFMIAVRQQDGNMLTNESGETTSHLMGFFYRTIRMVGSGIKPCYVFDGRPPVLKGGELEKRLKRREEAEKKAEEMKETGTVEELQKFERRTVRVTRQQNEEAKKLLKLMGIPFVEAPCEAEAQCAELSRTGKVYGAASEDMDTLCYSPAHFLRNVTAAEARKLKIEEFDIKKVLEGFDLDIHMFIDLCILLGCDYCETIKGIGPVTALKLIKEHKSIENVITSIEENPKSKYKVPENWPYKEARELFLHPDVLKGDDVDLKWTEPDVDGLVEFMVKENGFSEQRIKDGAAKLSKALKSGTQGRLDGFFKVSTKVEKVVDKKRTAKADGKSAKKQKQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU59</accession>
    <name>A0A1E3NU59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14577</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020206.1">
        <property type="nucleotide sequence ID" value="XM_019160444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU59"/>
    <dbReference type="STRING" id="763406.A0A1E3NU59"/>
    <dbReference type="GeneID" id="30177131"/>
    <dbReference type="OrthoDB" id="332281at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="102"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="327" mass="37104" checksum="3E65CC29900C7C74" modified="2017-01-18" version="1">MSSSLADYRLREHRTSRNLKTENGSGIEEDPLTPIQSQSTEIYENGIDSNLGLGLDLELADAPDTELSDTSSVSFELYDDKFSREKLISQIKLENGTRDRGKDLNIGEFLLKHYKYRELGDISDGLAELIKGIDDDLVELVNVNYLSFINLGKSVDGSLDLIHDIKIDLSDYLKNLKSSNKAIEEDTTKLDTLQDSRGRLLSLRSIVERLITLSEMIECFDKLCSKFDKNEECTDMLKELVGLYFGINKMFLKLIQQTKDENLVSLANSGILLGLSKKMNGLKLEFKSLLGSYLTHLRDTNPSNEEIFEIFKLYQLLNLTEDFKKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDE9</accession>
    <name>A0A1E3NDE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015262.1">
        <property type="nucleotide sequence ID" value="XM_019164930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NDE9"/>
    <dbReference type="GeneID" id="30181617"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF36">
        <property type="entry name" value="GLYCOGEN-BINDING SUBUNIT 76A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="389"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="586"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="732"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="823" mass="91077" checksum="B8CBDE233B853DA2" modified="2017-01-18" version="1">MPYVPSPRKRRASQDGGHDTSGDVVPPARAASGEQQPATATDVLTSVDRQLSACSIPLAGRLNEETCDQTSSTAALASEQAHSRSSPCGQQFDGHAVGFGADPAFPGNSLFSGDEDNVPLSLSFIRKPSRSSSMTVDVDRIMKQNTLMNSRSFELLPTEPVRDVLAEHSNSIPIAPGDDTPFPNDENPVSPGSPLSPLCSQTNIDTETFNTNYNNNYSYPLIRKKSGELVKSSLKLNNAFQASGAVSLPSTPTYKQVHFGNSIAIRYFDQKDKPNSISADNSPYNSDLDDGMSDNELQTGVYSDDEDDDDDNDTNNEGTDSVDYYGFSQDDGNDDGNDFKMTRCFCEKLYRVSKKLVESDTPVNTEKFKNVMKNWNLDTSQFKALSYRDQIDCEVPVFLERCFLNLDKTLIVGQIAVKNISFTKIVKIRYSFDNWFTIVNVDAKYTSDIPRVLKRAGYDRFLFQLSTPVLLSMYFKTNPNFLNLAPNFEFCIKYTSGGQDFWDNNHSRNYRLSFNHNNFLKLNKAASPTPKKTTSFDDSYFSFTPKNVRQSTDTKVADSQVPSIPSPSDYIPNQTFSSPTSSSNQLHPPRSSSNSSLNSHTNSTLSPPATLKKSRSFDRRNSIKSLIGSPEFLVRDKDKYKSNGSTNSDNSEPNSIDLNRGGLKNNFKNSGNQLPSSNSQLKNENENILVKLKELELNPKEAGKTSLVLPMTLNTSSSSSRIINGNNPTLKDSTEYPSLEKSEDSNSTVDNQGLRKPPGGADTENGGNSEGLISQPGWKGVDSETYKDLLEKYCFFKGPSTVSSFLADEQEMNGNNLYDDKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDP2</accession>
    <name>A0A1E3NDP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015368.1">
        <property type="nucleotide sequence ID" value="XM_019162030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDP2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDP2"/>
    <dbReference type="GeneID" id="30178717"/>
    <dbReference type="OrthoDB" id="199771at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11608">
        <property type="entry name" value="eIF2D_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21156">
        <property type="entry name" value="PUA_eIF2d-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039757">
        <property type="entry name" value="EIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039759">
        <property type="entry name" value="eIF2D_SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048248">
        <property type="entry name" value="PUA_eIF2d-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057429">
        <property type="entry name" value="WH_eIF2D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26292">
        <property type="entry name" value="PUA_elF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25304">
        <property type="entry name" value="WHD_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="2">
        <location>
            <begin position="520"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="604" mass="67451" checksum="9E39FB02918DAB03" modified="2017-01-18" version="1">MFKKHLEVKPSSNIKSSEKRRLQEDVQKHIEGLRFPGKLSKAFFNSNEVSKGTLFFDSETKDPVFFQLRDGKNLVPTLHELWASVHGSEPLQMPVIMTHEAVIERLVNGSDLMIRGCIEPYGSGLRSGAVVAVVDYKTPNVAMAVGKCLMDLEGKREDEIPDSGKVVQVWTVVGDHLASLGRSMDEVLKEAERRNVQEQQQKGDAETQTEDAEHALATVSLDEKDEKDEKDEKEVEELKKEVEVKVADAADGEDHDEPDEYVMSTEDIDEMFRRAVLYTLNQDKLEFPLLASQFMSGHVLKNLPPVDDSVVNMKRTSWKKSGKFLKAMEKETLLKLKGKDDKLSVVSVAPRSDPRVLNFVPYRIQGLATQTAAKTDAKTTPGTAASSEGAVPPLVVKTYLKPSNASRMMFNRLDAVYDYYYTENEIKAFVQRYMRQNPQLVCRASPDYIEPDDILGGFVLADVRRVKRVDMVNKVVASCTLYHAVYREGDAKSNDVLVKKRLVPKRGKPPTVSITIESVKVGRRVVTRVSGLDAFYVDTDRLADVLKVKCSGSCTMSDDPRRGPTLGVQGSHDAAVISVLSKDWGVPSACCTVDNKTSFRKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDQ2</accession>
    <name>A0A1E3NDQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18505</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015378.1">
        <property type="nucleotide sequence ID" value="XM_019162032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDQ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDQ2"/>
    <dbReference type="GeneID" id="30178719"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062040">
        <property type="term" value="C:fungal biofilm matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="610"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="613"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="646" mass="70471" checksum="C23993FFB19149A5" modified="2017-01-18" version="1">MSKAVGIDLGTTYSCVAHFSNDRVDIIANDQGNRTTPSYVAFTDTERLIGDAAKNQAAMNPHNTVFDAKRLIGRKFEDSEVQTDMKHFPFKIVDQGGKPKIQVEYKGETKIFTPEEISSMILTKMRETAEAYLGCKVNDAVVTVPAYFNDSQRQATKDAGSIAGLNVLRIINEPTAAAIAYGLDKKTEGEKNVLIFDLGGGTFDVSLLSIEEGIFEVKATAGDTHLGGEDFDNRLVNHFATDFKRKNKKDLTGNPRAMRRLRTACERAKRTLSSSAQTTIEIDSLFEGIDYYTSVTRARFEELCQDLFRSTLDPVEKVLKDAKLDKSSVDEIVLVGGSTRIPKVQKLVSDYFNGKEPNKSINPDEAVAYGAAVQAAILTGDQSSKTQDLLLLDVTPLSLGIETAGGVMTKLIPRNSTIPCKKSETFSTYADNQPGVLIQVFEGERARTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSALEKGTGKTQKITITNDKGRLSQEDIERMVSEAEKFKEEDEKEATRIASKNQLESYSYSVKNTSSEKAFEEKVSAEEKEQITKACDETIAWLDENQTASKEEFDDKQKELEQVVNPIMTKFYQAGGAPGAAPGGAAPGGFPGAGPAAEEPAGPTVEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDS8</accession>
    <name>A0A1E3NDS8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEY1</name>
        <name evidence="12" type="ORF">PICMEDRAFT_56346</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Enriched in the cortical ER. Concentrated in punctae along the ER tubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015369.1">
        <property type="nucleotide sequence ID" value="XM_019163186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDS8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDS8"/>
    <dbReference type="GeneID" id="30179873"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="698"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="721"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="723"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="745"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="45"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="619"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="627"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="55"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="800" mass="91660" checksum="24732E80C249EF4D" modified="2017-01-18" version="1">MSSSGNHDTATVSVHLVDEEKRFAKGLDKYVEKCYVGDDHGEDDGLNYHIVSVFGSQSTGKSTLLNRLFGTQFDVMDEEKRQQTTKGIWFSHANYVASAAEEQEAGRRRNSNNIYVLDVEGVDGRERADDKDFERKSALFALATSEVLIVNIFEHQVGLYQGANMELLKTVMEVNLSLFHEQSEKCLLLFVIRDFTGLTPISNLGESLEADMKRIWVDLNKPDQCADSQLSDFFDLKFFAISHKHYQPDKFEYNIRQLGDDFTNDRLFSHNAYHKRIPIDAWALYSEKVWDQIENNKDLDLPTQQILVSKFKCNEILNSVYDDLFLQEYSAVQEPELDPAASCAQFKELRHKCLSRYDSQASRYKGAVYSDVRRDLKAKIDLKLKGFQSTILSNLSKSLISKLDTGFASLKKTNSKKSFNYSLAGEEEKGAEYGADTSVFVENYAVELEKLEKALQELSHTLRTKESHILIGKITKKFQTKFKDCIIEELSEPTPESWDHIFSKFNELMEKIFKPYRSGDSFDFKIGLSQDENSTVHMKVLRNIWNKFDSIVHDYINDDSASRILRNLFEDSFKFDSNGLPLIWKTFSQLDSQFNKSRDHALSLFPVLCTIKGPEGSTIQKPKFDIENEPNEGIDSDDAGGENSSDEDDSENFEKSDRNSRFLTLLTPKQQAKVKARFKKETDAIYLDAKRSMVANKTSIPTFMYVLLILLGWNEFMAVIRNPVLFILAIFVITGLYFAYNLQMLGPMLTVTNAMVQQSKAVIKEKLRDVLLDDNQKAPLPTELEKEEVHIEEVYELHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE66</accession>
    <name>A0A1E3NE66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015522.1">
        <property type="nucleotide sequence ID" value="XM_019161948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE66"/>
    <dbReference type="GeneID" id="30178635"/>
    <dbReference type="OrthoDB" id="3993369at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="357" mass="41412" checksum="20290B9272F9B33E" modified="2017-01-18" version="1">MRSTHLSRPGSLKDDPLRPYGEKSLKRARTIAGTSTTASTLQMTQLSGLTVLSEINTNIREKSYSPYMRSRLRENVSGIFLQDENLGIKINGDSELEQKNSLKSKSIPGLYPRLSIEGKISPEARIPSLKNQNLFDEINDEPEIEQSSSKSFVTLISKNFNGLSTVKNTFCSEEENLYDDESEAKHNRQFTSVEFDVPKITLLDDSAPLDLSQVKRRLVPYASPFFQTIEFNPFEPTDTGILLKILNHEFESLDDENDDDLMQEKENKRIPWYRELLNLEKYYTQFINCFKPLSKDEYNSKYPWDKFYDPELYYDNEMDDEGVLNTELLPENLHNSISSEINDYMKPYSYISRRRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEN4</accession>
    <name>A0A1E3NEN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartate protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_18004</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015703.1">
        <property type="nucleotide sequence ID" value="XM_019161845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEN4"/>
    <dbReference type="STRING" id="763406.A0A1E3NEN4"/>
    <dbReference type="GeneID" id="30178532"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05488">
        <property type="entry name" value="Proteinase_A_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000036">
        <property type="entry name" value="Vacuolar aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033819">
        <property type="entry name" value="Saccharopepsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133332" description="Aspartate protease" evidence="14">
        <location>
            <begin position="24"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="98"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="129"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="335"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ44590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="412" mass="45156" checksum="A311314FE4932485" modified="2017-01-18" version="1">MNFKSSVLTSLVLMLASLQGADAKVHSAKIHKHPLEESLKDVSFVDYVDSIKGKYMKNFVSSVNAPYVPFVETIMDEELEHIEKTHDLPLTNYLNAQYFTEIQIGTPGQTFKVILDTGSSNLWVPSAECSSLACYLHEKYDHDSSSTYKKNGTEFAIRYGSGSLEGFVSQDLLTFGDLVIPKQDFAEATSEPGLAFAFGKFDGILGLAYDTISVDKIVPPIYNAISQGLLDAPQFSFYLGDTAKSEEDGGVCSIGGYDKTKFHGEITWLPVRRKAYWEVKFDGIGLGDEYAVLEGHGAAIDTGTSLIALPSQLAEILNAQIGAEKSWNGQYTIDCAARENLPDLTFTFDGYNFTITPYDYTLEVSGSCISAFTPMDFPDPIGPLAIIGDAFLRKYYSIYDLGKDAVGLAKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF76</accession>
    <name>A0A1E3NF76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_18178</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015904.1">
        <property type="nucleotide sequence ID" value="XM_019161904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF76"/>
    <dbReference type="STRING" id="763406.A0A1E3NF76"/>
    <dbReference type="GeneID" id="30178591"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF59">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A, GOLGI ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="469"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="847"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="752"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="752"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="924" mass="104954" checksum="2B65525032DF6D34" modified="2017-01-18" version="1">MPAAQKEAFFRSADMSLVQLYIPSEVARDCIAILGDMGDVQFKDMNQNVNSFQRAFVKEIRTFDQCEKQIKYLSDSIRKQNIHLPTTSYDHLVNPPSLDYHLFENSQSHLTSSQLDELINVINTYEANIRQMSNTNDDLLKRQHELIQHRMVLRNTRQFFDARLSLEIDDGDSIFNQVRLSSESTNDSFMISDDEDENAHANGNGILLESGRNNASATNNVVATGANELNVLGSTMNFISGIIDTDKFMILEKICWRTLRGNLYINHVPLDEPLLDIATGEKSYKSIFLIFTHGDSLISKCKRIIESLDGQLYDVESDYEAYKHEMRTINDKLKDLNEVLLHTEDRLLLELKEIASEIEKWKITFRREISVYDTLNLFNYDSARRCLIGEGWIPIDDLPYINAALKDVTDRVDMGLSTIVNVIKTNKTPPTYHKTNKFTAAFQSIIDAYGIATYQEVNPGLATIVTFPFMFAIMFGDLGHGLILFLAALTLVLKENSIGKMKNRDEIFDMAYTGRYILLLMGAFSMYTGLIYNDLFSKSMTIFRSGWKWPSDFAEGQSIVATQSGVYAIGLDPAWHGTDNNLLFTNSYKMKLSILMGFIHMSYSYCFALVNYKFFKSRVDIIGNFIPGLLFMQSIFGYLTLTIIYKWCIDWVGIGKPAPSLLNMLINMFLSPGTIDDQLYPGQGPIQVILVIIALVCVPWLLLYKPLTLKRMNSHIIQLGYADVDSGSNAHSRFSIETVTSTETGNNLLNPFSDDSTIEPSHTANTENAEGESFPSIYDEDGDEPEPFNFGDIMVHQVIHTIEFCLNCVSHTASYLRLWALSLAHNQLSAVLWEMTIQNAFSPYNQVGFLGSIAVFLLFGMWFVLTVCILVVMEGTSAMLHSLRLHWVEAMSKFFEGEGYAYEPFSFYRILKDMETAAFEEERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFN1</accession>
    <name>A0A1E3NFN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_23750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I NDUFA12 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016035.1">
        <property type="nucleotide sequence ID" value="XM_019162321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NFN1"/>
    <dbReference type="GeneID" id="30179008"/>
    <dbReference type="OrthoDB" id="10255576at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052618">
        <property type="entry name" value="ComplexI_NDUFA12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32470">
        <property type="entry name" value="ADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32470:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="17060" checksum="831474A95CA43A85" modified="2017-01-18" version="1" fragment="single">HKKVPVWKQLYYRWRSLRGVPFRRKFFVGYDLDANTYWEFFLDRGQVRGRRVVQPHEPQKMLFSYFDKVPIQWAQWLKYARRTPPSIYDLIADQERLKKLQIMASFRDSEQLYNRQLKEEKIDQTLQKELSKLEAASQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFP0</accession>
    <name>A0A1E3NFP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_37624</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015276.1">
        <property type="nucleotide sequence ID" value="XM_019163095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFP0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFP0"/>
    <dbReference type="GeneID" id="30179782"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080139">
        <property type="term" value="F:borate efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF82">
        <property type="entry name" value="BORON TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="26"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="198"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="552" mass="63221" checksum="5805B89F9CB34D36" modified="2017-01-18" version="1">MFTAGRSCIHTYYDRYKANDYFRVRYFGTGIYNDIRRRLLKYYVSDIKDGLTYRTIPSTIYIFFTNLLPAIAFAQDMFDHTNNSYGVNEVLLASAMGGIVFGVLSGSPLCIVGVTGPVSIFNYTVYEIIEPMNIPYFQFMCWICLWSMGFHFILAFTNSVNFMRFVTKFSCDVFGFFINIVYIQKGIQILTHQFELADPASAFASIVVALVMTIFGVSSQFFGSKSHYLPPRIRTFIRDYSVPLCVVFFTGFIHFGGYLDDIKFTTLPITKSFHPTDTVNRRPDWFIRFWEDIRVRDVFLAVPFAILLTALFYYDHNISSLICQSSKFPLKKPSSFHYDFFLLGVTTGVAGLIGIPAPNGLIPQAPLHTHSLCVYEHDYETGLNKITRVVEQRLSNTFQGLLTLVMMTRPFLVVLGLIPQAILAGLFWIMAITTLDDTDVVRRLRFLFLDKEWKLKLSKIIIPADYPIEYYLVRSKYFIIYLILELLAFAGEYSITCTKGAIGFPGVLMLILLLVPIVFPRIFPKDQLDLLDSEAADELIMRSLKLSSFNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFS0</accession>
    <name>A0A1E3NFS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_73760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016075.1">
        <property type="nucleotide sequence ID" value="XM_019164577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFS0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFS0"/>
    <dbReference type="GeneID" id="30181264"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046827">
        <property type="term" value="P:positive regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010603">
        <property type="term" value="P:regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 8 9" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="124"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="242"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="356" mass="39048" checksum="C1EF2E9E2D63B9B6" modified="2017-01-18" version="1">MSSDQVFSFQSKAPSFKNSFDPSSDPLDPHSKAVSQPPQPPSQAQAQPASQQAAPAAGLKSFFGVGAANDGASNFNFNAHRRVSVSAEALNPNTFSKDNWKPPVHQLSHDQLTRLNASVIKNFLFSQLDEDSLRTIIFALEEKRCPKGFEIIKQGDEGDFFYVLEKGTVDFLVDGKVVNTSSDGSSFGELALMYNSPRAASVIATSECILWALDRMTFRRILLEGTAKKRSLYENFLKEVPVLQSLSPYERSKLADALNTESFKTGENVVSEGEAGENFYFIEDGDAEVIKEGHGLVTTLKKGDYFGELALLYDSPRQATVRALTPLKVVSLNKSGFQRLLGPAVDVLKLHDPTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFT5</accession>
    <name>A0A1E3NFT5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_37297</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015541.1">
        <property type="nucleotide sequence ID" value="XM_019163075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFT5"/>
    <dbReference type="STRING" id="763406.A0A1E3NFT5"/>
    <dbReference type="GeneID" id="30179762"/>
    <dbReference type="OrthoDB" id="364224at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000017">
        <property type="entry name" value="SEC13 homolog (S. cerevisiae)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="6 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="877"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="1023"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="656"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ44428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1124" mass="124833" checksum="86D486E7BB706ED6" modified="2017-01-18" version="1">MESIGNGNSGAGKPGHPGDPTPGQQGQGQTAAQTQAQTATQTPRDVRLLRLIFATQGVTAYEDQVPLQLMDFAFRYTSEVLQDAMVYNDYAHPTHGDGSGPNNQMLTNEDIRLAISARTSYQFKPIPPKKMLMELAAERNEKPLPAVMPMWGLRLPPEKYCLTAKDWSEEHDTAPAEKRRKTTAILSQINDLGPPDLVNLTKTVGVPTSSNASSLPSIGTYLYYTGADTSNVATIAALLNSLATIIGQELQLWFGKPKPFHVSHATYSTYNAFRKLDLRVSVAFPGSVSWEVLDAWGNHASSEIVPPEEVDVMWEECFVSAIVRSLITSDDDGDFSSIVEIRRINPFLDPKVSVSTFLTGFEHLYPDGEKLGCNEDIQVPTRLNNYLVDSFIKCVELTGCFDEAMTVLQRLYEKYPEVAAVIAKVLFISNHELEAVKFIHDKLSHPTSVAEIPGASQLLLLQSEYCLEKSRADLALPLAVRAVESSPSMFAPWAHLVKIYISLNKFEDALLTLNACPMVTHKDKYILKRIASDASSASSASTSQPTSSAPLASDMHLPLPQDVFLRGVTDLNSLDVAAEHSKLTKPGGPNNRRSSEVNSIVTPQSLLTLPAGSLKSTFRRAYSLLADIVHRLGWEGMLNIRSKVFVMEEEWRRDPKVASSAESSNPGESKTSADLPTNGSGETTDEFRKKRLCERWLDNLFMLLYEDMKAYTYFDPYTESRSCLEWELLGLVSERLGHVRDAQRCYERALARRFSVRAAKKLVNTSTIINNHVVHIRDPMRYDVALLRLTVGLLVWDYRWYTLFSPLLLNTLSSVVNDMGVTKVESEVRVTIGNAHDDLIHDAVLDYYGKRLATCSSDKTIKIFAIENDQHKLVETLHGHEGPVWQVSWAHPKFGIILASCSYDGRVLVWKEENGVWSTITEHRVHSASVNSISWAPSEYGALLLCTSSDGRASVIEFLADGNTKHFVFEAHSIGANAGTWAPCIGDGKKMEDRRIVTGGCDNLVKIWKFNTEANNVILEDVLQGHTDWVRDVAWSPSNLGKSYVASASQDRTVLIWTKETSPDGTPSSPWKKTYLTKDKFPDVCWRASWSLSGNILAISGNDKVTLWKETLEGDWESAGEVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFW1</accession>
    <name>A0A1E3NFW1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_59864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016148.1">
        <property type="nucleotide sequence ID" value="XM_019163395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFW1"/>
    <dbReference type="GeneID" id="30180082"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="124" mass="13317" checksum="340D516B81A17FAB" modified="2017-01-18" version="1">MASLSLEYGPRGGDSTGQKRLQDIKQTRLLNRTAGYPVQVIIANAIMAAKTSPSFFSCRWSYAAGYAGSTLVKGKSSIAVLRQDLPPRVGPRRQVLSSIVDYSKLGISLAPSLPQPRPSNNISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGA2</accession>
    <name>A0A1E3NGA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015486.1">
        <property type="nucleotide sequence ID" value="XM_019161933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGA2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGA2"/>
    <dbReference type="GeneID" id="30178620"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF275">
        <property type="entry name" value="LD06095P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="152"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1004" mass="112652" checksum="935D46885FBA8A9E" modified="2017-01-18" version="1">MSNQVYSSKSLLDAIGSEARKSADRKASEPTEEIENDGGSFLLANTFAHVPKPEQEGNNSNEVSKEPLKDNETKGKDIGKEDIVGKGKLTGSDVESVTSGIVMNTSEEKDRTKESKTIQLLPLLNPSNLLSSHDLLLSAFYEEHKYELVEQRKLTMRVITWNLNQMKPPSLSSLAGKSGREWAAFFYAGDATVNEYNKEGLADIYAINFQETISLKSFTKSNGVIDEWVTFLLAVLNAISSDSYSLVHKTGLLGLTSILIAKSNLTADNSDDTDGHIHDIRENTLGLGYLRWANKGCISLRFRVGGLSLGVGEYKSRFIAATDDASYTYKFSELDETAGKIPGLEIQVLNVHLVHGEDSSQIQQRWDSWAKIESKIGLDDRTVKLALDSSKMEVKDAAQKRVEMKLQQKLHKESNLDADNYEHRMDKEMKALNLNNDAIRTSSVPITFSATELKKFDGIFSSVDIKNLKYVTEAQKALVVCGDTNYRLSLPTEATSNLQKVIHELIKNGRWNDLIQHDQLSREISLKKIFVGFKEPEIHFAPTFKVKNDCIGNWIITPPSSSSSSSPELVVKPNTSTSKLKRRPVPVPASPSGDSAPSVEPIPFYVPLYDTKRLPAYTDRILYVPRPYFNVIEGTYTSTGNSGSDHLPVASTYEFEAPLIDYNKLHVLRGKFTEAWDAVINQMKFIEVNNKVTINHSMESMKAQYNWQTLAPCISETVSTDGGNVAFSAIVGETIYIIINIENVVDESFNIYVKEQTSGGWFGTKIGVNCQQVQSYNKTEFPLRNFSPSCNIESHSKGEIIFSLVPTNSGLLERMFVTEIPEYSLCPAYRKFIALTINVEDIFGISFENITPQQFENLTRCFDFVCSEPIKGLLERMEDLGSSGDLNSADWELVREISLWKFSPEKYKQQNIDGVEAVKRREPEKFNLGSKTVMSVIYIWLKSQSSSFNTDSPRGKVIFGKVIKLVKHLKLDAEDGYSWFGWLFGNEYELGDYLERDFDVKVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGI4</accession>
    <name>A0A1E3NGI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_168426</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="4">The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016335.1">
        <property type="nucleotide sequence ID" value="XM_019161357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGI4"/>
    <dbReference type="STRING" id="763406.A0A1E3NGI4"/>
    <dbReference type="GeneID" id="30178044"/>
    <dbReference type="OrthoDB" id="202234at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004552">
        <property type="entry name" value="AGP_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00530">
        <property type="entry name" value="AGP_acyltrn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF11">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="7">
        <location>
            <begin position="80"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="303" mass="33654" checksum="5573796AC239CC62" modified="2017-01-18" version="1">MSIWGNFFKKFKFVTKGIVALNVLLLSATYGVLCSVYFAITRRRNLAQWSTARFYYNIFSFVMGMRINIDKPELLEKLPAILISNHQSELDIYMLGRIFPPKCVVTAKKQLKYLPFLGWFMSASGTFFLDRSNRESAIRTLNNALDQLKENKGGLFMFPEGTRSYSSTPTLLPFKRGAFHLAVQAQIPIIPVIVSNTSNLYSMKLFNFNRGEINIKVLDPIPTLGLTKDDVPGLTEKVYGMMNQAVKELGMSTVYGETIALNTQDAVEEAGAGHADADIEANATATGTTETTAVSESTSLLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH28</accession>
    <name>A0A1E3NH28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_35819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016563.1">
        <property type="nucleotide sequence ID" value="XM_019162967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH28"/>
    <dbReference type="STRING" id="763406.A0A1E3NH28"/>
    <dbReference type="GeneID" id="30179654"/>
    <dbReference type="OrthoDB" id="266138at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051457">
        <property type="term" value="P:maintenance of protein location in nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38477" checksum="4780D2C0CA0895CF" modified="2017-01-18" version="1">MAYIEKTYGLTTIPDTNVDVLPADQELTTDIPLDTTYIDLIHLKIGSLENLRLERFTQLESINLRDNLIVRLNALKHIDPQCKLRIKELDFYDNRIKHISSHVNEFTNLEILDLSFNNIKHIKHVENLTRLENLYFVQNKISKIENLDRMTLLQNLELGGNRVETIENLDSLVSLRQLWLGQNRISRLEGLARLRNLRVLSIQSNRIEKIEGLDGLTALEELYLSNNRISKLEGLDALVNLQVLDVTSNAIAKLEGLDALTQLTDFWCSYNQVADYSNIHDALQHATSLETVYFEGNPIQLKDPTMYRTKLRLCFGDSLQKIDALYVSTGRMVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHI1</accession>
    <name>A0A1E3NHI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcriptional repressor Tup1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_22649</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016700.1">
        <property type="nucleotide sequence ID" value="XM_019162272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHI1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHI1"/>
    <dbReference type="GeneID" id="30178959"/>
    <dbReference type="OrthoDB" id="17410at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036033">
        <property type="term" value="F:mediator complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000879">
        <property type="term" value="P:negative regulation of dipeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042304">
        <property type="term" value="P:regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000111">
        <property type="entry name" value="Transcriptional repressor rco-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013890">
        <property type="entry name" value="Tscrpt_rep_Tup1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08581">
        <property type="entry name" value="Tup_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcriptional repressor Tup1 N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="345"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="380"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="421"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="470"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="522"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="564"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="605" mass="66897" checksum="CBC153CEA5578C2B" modified="2017-01-18" version="1" fragment="single">NSSNNTNIMPSRAHLDELLEAIKGEFDNLTRETSRYQDDHHEYEIKLNQQHQELTNISNTVYELEQAHRSMKEAYEREIMKLKQELEVRDRLLEQQSLQQQAVAAQAQAHAHAQAHAQAQAQAQAHAQAQAQAVQTLNPAPYSQTPQQLPPPNLSSTSPPAYSNQLNKPDIFNNSGSSVDKKTNNTLAPIAPLASSLPNPASNEATSPSSSKTATYSGSKSLTKPQPAEESLIKRAQHNKPIPAFLKDFDAFGSLPYKKQHNDYYLVYNPKIPKKVDISLVHSFDHTSVVCCVRFSKDGKFLATGCNKLTQVFSVETGDLVARLNDESSVSTNGGYDTDTGDLYIRSVCFSPDGKFLATGAEDKIIRIWDLATRTIVKYLKGHEQDIYSLDFFPDGKRLVSGSGDRTVRIWDVYNGQCSLTLSIEDGVTTVAVSPDGSLIAAGSLDRTVRVWDANKGYLVERLDSSNEYGNGHMDSVYSVTFTNQGKEIASGSLDRTIKLWSLKDLDKQPSSVSKSNCEVTYVGHKDFVLSVCCTPDDEFILSGSKDRGVIMWDKETGEPYIMLQGHRNSVISVSVSPVLNPNGGYFATGSGDCKARIWKWERIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHU1</accession>
    <name>A0A1E3NHU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase I subunit RPA49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73205</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016804.1">
        <property type="nucleotide sequence ID" value="XM_019164414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHU1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHU1"/>
    <dbReference type="GeneID" id="30181101"/>
    <dbReference type="OrthoDB" id="532500at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="50421" checksum="F37744934343EBAC" modified="2017-01-18" version="1">MGKNVSIGKVDVVSEAGEAVVASFTNSTTIPGSVSLDLYTSTKAPNKAILHGESKDSALQYDADELADSQYVLGVYDSKSKSIKLIPRTKMFSGRVNASRSLISDAKLVKKLKRKDISAEGTFAERRNALGEEFGTKKAKKAITEAARNKIDAGMLEESQIDIVDGIRQTTKSMPDRDQMAKMVEKENRVIPPCHTEATNVEEIYPIEEIVPEEILQCFPIDIFFRAKVEGQEEEEALEEQKETEKDEVLQKLNFLPYVPAKERGPVHQKKSTFGRLIASALSERSSLSGSSNISDDARWKLQMISFTSMLVGLYFNRRLSRRDKLMTAYHNVPPSRAINYMLQVFGNTKVSSNSFDREIRFFNVGPKEEDKLLCYIIVLLLSLFEYRLELSSLAADLSLKPSKLLALVRTLGCTIVVPNKSESKDMAGSKIAVLRVPFKVPDMVRRFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHU8</accession>
    <name>A0A1E3NHU8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016251.1">
        <property type="nucleotide sequence ID" value="XM_019164620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHU8"/>
    <dbReference type="GeneID" id="30181307"/>
    <dbReference type="OrthoDB" id="3998262at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF10">
        <property type="entry name" value="OS12G0136300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="669"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="693"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="750" mass="80952" checksum="518FAB7581D813A5" modified="2017-01-18" version="1">MPKQLADGEYDLDFSGLFSTVQSDVFGIRYGFKPDIIDDGFPSFLYKDVAGNARQASPTRGNETVMVDPGLSATEIPFYLVSESKESTKAITTNLSKDDRIYFEGRSRPNAGLHQLMSTAMTPTGTGSTSSVALENGAGAGAAHFGSMSEFLLSYDDVSKSFKVDVFNGFIKMNKSREPEIISGKINKMTHRQTKLSNAPSSAAATISPMTKSYIDSLITSFASGTSSKPSLSCSRRTTSPVRTANIIPTEVGMIKPYGKSISGISPVRRHVSNSPINSPTRKVFQPSKTLSSVPVSAPPSTPAKPQSPLKVSASPSPVLQTAKKNVITPTNGGTGTRKTQRSLLLRKAERAVGINKGRVYKPKLKEPALKEKEILSSAVDASHKTHESTGLRIRQRELKEARPDQAAFELVLTPPPSVPPKKEVSPQKPAKKAAKVLEKDVRKVTKTEPTLEAWKDPVLSDDLDLESFAEELESEMNTASAAEEADTTSLTTKSAKNVKSQKAEAINTTPSLGVETYPTAISPTPPLPPSQSPAVKELKVNPAKAIDRTTDRTTDKVAKTFDDDLNIEFSDWEDADAIDGPVLSDENELTAGNSEFQLIIEDDPLKSKEMKERKQIHKEEPRPDKLATFPVKQVKVETKSAKSVKPAKNKNTVKLPTPTPTPAPASAPSPTAAAPPAKKNTTPASSSATPKKAPKAKGTGKLKKKTGTPQKARHSGLPHEDDEIDRELDAELDKAFDDLLDEEDMSEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI36</accession>
    <name>A0A1E3NI36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016116.1">
        <property type="nucleotide sequence ID" value="XM_019161627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI36"/>
    <dbReference type="STRING" id="763406.A0A1E3NI36"/>
    <dbReference type="GeneID" id="30178314"/>
    <dbReference type="OrthoDB" id="444265at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="142"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="240" mass="26603" checksum="0709EDDF811138A3" modified="2017-01-18" version="1">MQQHGSRRYGERRSGKHDRASQSGARHTRSGGEGGGRHKHRERDGKGLQWQDGGEGGQRKPAGVSAATTPIPIDLRPSGVLTKYLHLSAGQKVAPHDARAKYAPPEDAVVPTKENCAFHLFKYNEETDTQEEVPLLNYKSYFVFGRDAELADVVVADDEDGDLVSKQHAVLQFRRGRDATADAASVRCYLMDIGSTNGTFLNDDKAELPTKRYVQLKNNDVFKLGDYDSVLEFMVVEDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI63</accession>
    <name>A0A1E3NI63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_145914</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016943.1">
        <property type="nucleotide sequence ID" value="XM_019160450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI63"/>
    <dbReference type="STRING" id="763406.A0A1E3NI63"/>
    <dbReference type="GeneID" id="30177137"/>
    <dbReference type="OrthoDB" id="6683853at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.210:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="92" mass="10655" checksum="8FBCEEB1250C7228" modified="2017-01-18" version="1">MTHPGAYMGWWGSMGSPKQKRVTIHSVSPYAQAPLHGSTERAIFNSFRRFKSQVLYIAIPFAIVWSVWTDARDYNEYLYTKAGREELERVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIA6</accession>
    <name>A0A1E3NIA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS15 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17118</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016964.1">
        <property type="nucleotide sequence ID" value="XM_019161471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIA6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIA6"/>
    <dbReference type="GeneID" id="30178158"/>
    <dbReference type="OrthoDB" id="623277at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="141" mass="16027" checksum="B0EE4901426520E2" modified="2017-01-18" version="1">MSSSSLPYSRALPSWFKASSDEVVDQIIKYARKGMTPSQIGVLLRDAHGVNQAKIVTGNKIMRILKSNGLAPELPEDLYFLIKKAVAVRKHLERNRKDKDSKFRLILIESRIHRLARYYRTVSVLPPTWKYESATASALVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIB3</accession>
    <name>A0A1E3NIB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016974.1">
        <property type="nucleotide sequence ID" value="XM_019161474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIB3"/>
    <dbReference type="STRING" id="763406.A0A1E3NIB3"/>
    <dbReference type="GeneID" id="30178161"/>
    <dbReference type="OrthoDB" id="3996113at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022592">
        <property type="entry name" value="Nucleolar_19"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10863">
        <property type="entry name" value="NOP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25145" checksum="FA42956FE55B72F8" modified="2017-01-18" version="1">MSKRNEIRQKEQLQVQLQAQFNKLDQTVLSWLTKPAGESKPETPPLTKLTSADKTDDFKPQSDATQQFINQIVIPSGKGINFSDLEPKNNNNSSMVTINDFLDVVSAKRNSSSSRRETNGKGGMSEKVNKIGDVKRRTNNSNSLRALSNKLRDDRREKLRTNIRKEGPNNKYDLKKHSAKVTTKLNTNRSGSESDSEIDDEILHRKSKSSGMLAKATKNKRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIP3</accession>
    <name>A0A1E3NIP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17703</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016326.1">
        <property type="nucleotide sequence ID" value="XM_019161703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIP3"/>
    <dbReference type="STRING" id="763406.A0A1E3NIP3"/>
    <dbReference type="GeneID" id="30178390"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF4">
        <property type="entry name" value="ARGININE PERMEASE CAN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="99"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="601" mass="66873" checksum="B3A8D93EAE505292" modified="2017-01-18" version="1">MKFDVLSKFKKQADVHEDVRSIGNSSPFSHDTEMGTADPGAELYMISSNNEIAAAKSNRSIEKLSEKKSAYDIDDINSQDDIEGYVHETKVKRGLKQRHVSMIALGGTIGTGLFVGTATPLQLSGPVNTLIAYLFFGALAYSVTQSLGEMATHTPVAGSFCTFNTKYLSKAIGFATNWCYWFSWAITFAIELFAVGQIIEYWTLAVPTWAWILIFYFVLTGANFVPVRYYGEVEFWIAFIKVIAIVGFLIYALCMVCGAGVTGPVGFRYWRHPGPWGAGAGLVKNKNTDRFLGWVSSLINAAFTYQGVELTGISAGESANPRKTVPKAINKVIVRILLFYILTLFFVGLLVPYNDPALASTTNFISSSPFLISITNSGTPVLRHIFNAVMLATIISAGNSNVYIGSRVLYSMAGTTAPKFFALTNRHGVPYVGVITTSLFGLLAFLNVSNSGKTVFNWLMNISALAGLISWCFITAAHLRFMTILKSRNISRDTLPFKANFGPIITWITEFFLVLIVFIQGYAAFFNFTANDFFASYISLIMTVVFWIVAQLTYYRHDPWLIPTDEVDLDSEARNVDDEVWEDEEENEKKSLYDRFWDVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIS8</accession>
    <name>A0A1E3NIS8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_34331</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017155.1">
        <property type="nucleotide sequence ID" value="XM_019162877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIS8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIS8"/>
    <dbReference type="GeneID" id="30179564"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038074">
        <property type="entry name" value="Peroxisome_assembly_p12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="335"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ46042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="403" mass="46131" checksum="57331430708DC6AC" modified="2017-01-18" version="1">MDFYSSLNSSQLNTQKPTLFEVVSCAQLDSLLSPSLRFLLAHYTHRYPRLLIRVLNNFDELNSLLWGFIQYKYLETWNATFIEKFYGIKRISSRPLLPKSPEVVAANPAKYHEITKLSSLQILLSLIDTVGIPYLSTKLQLYHEQLIPQHLLDTVHLDVPEEEKSGLKKLLKEAFFKLYPSVKLSLKLFTILLHILYLSDKTSSVSIIQLLSKVSYARITRADHLRVESRFTPKEQSRDQVLNIPPTLSSVLFSNIQALALPLQKIAWATTDTILPVSIFLLKFLEWYNANRSAHEDKNSDNNKFIDAPVPPIVPPDALNSKEVKAAITTTDAACRICHEEIHNPAIIETGYVFCYKCIYEYLRDQPKETGGRCPVTGRRLLGCSWGETRNEWIIKGLRRVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIV0</accession>
    <name>A0A1E3NIV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_34744</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Prevacuolar compartment membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016621.1">
        <property type="nucleotide sequence ID" value="XM_019162905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIV0"/>
    <dbReference type="STRING" id="763406.A0A1E3NIV0"/>
    <dbReference type="GeneID" id="30179592"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF2">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="36"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="163" mass="19091" checksum="3AF848BE21FD300B" modified="2017-01-18" version="1">MTSANPFISFSEVTDGRGKKEHKQTFDELYGEPENFLEIEVKNPITHGSKSSNMYTDYEIVCRTNIPAFKKKFSKVRRRYSDFYSFRSILENETSRVVIPKLPDKSIFSYSNRFSPEFIEERRHGLEHFINVVASHPLLQTGSRSMICFIQDDVWDKTRFISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ00</accession>
    <name>A0A1E3NJ00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17282</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017159.1">
        <property type="nucleotide sequence ID" value="XM_019161531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ00"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ00"/>
    <dbReference type="GeneID" id="30178218"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="169"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="252"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="444"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="526" mass="57157" checksum="745C19A9770C7F0D" modified="2017-01-18" version="1">MLKRKNEESEESKESTHYVKRVALDAEAVERKDIQDDKQKTSVSLSGNSEEGGAGEEDEKVVGEDDGEERTGSADNNDDMQGIRENSGNEDAFDLGGNGADSEAGVGNGHENEVGGDESGESSKLVQTPSSVPSKGDFAAPKADSDGDNPQQQHGGPISSHRVQNDDPTYVHFRMLANISDTASIVGKGGEAISKIKEQSNARVNVSENLKGVPERIISVRGSAEYVAKAFGLIVRTINNERFDESSNIDSKSYNLRLLFPHTVMGYIIGKKGSRFREIEENSAAALRANDQILPASTDRILNINGVADAIHIATYYVAQTVIEHKQQISKAVYYNPANFNQPVLPALSMNQNHQHNNNNGNNRQFAQNGYNHQQNMMGMGNPNQMMSSMYNQNPMKMLNSPTGFAGMMHNGMSNNMGMMGAPQNSATGTANTTPGDTITGPDGKINQELFVPQKHIGLVIGRGGKNLKDIRQSTGCYVKVNDEVPGAPDRKLTLIGNVYGIQNAIVLINNKIENEKLRQQREGHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ51</accession>
    <name>A0A1E3NJ51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73641</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017287.1">
        <property type="nucleotide sequence ID" value="XM_019164539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ51"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ51"/>
    <dbReference type="GeneID" id="30181226"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF26">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_5G13940)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="351" mass="39310" checksum="2D7EF28942167E0F" modified="2017-01-18" version="1">MKKQVVIVGAGVIGLTCAWILSEKGFDVKIVSCRLPSNFKDETDYASPYAGAHFRPFPSSNAGDLKDYPLTRTSFNVFKKISKEFPESSVKFVKAIDYVEADEEPYTTLSPGYAEGLQSFEVLEKEVLPKNVKFGAKYDSWCLNAPLYLQFLERRLRMFYNVEFIIAALVSLKEVSQMYQGSTIVNCTGMGLTYKGGYDSNCYTVCGQTLLVRAPKDCIYNNQTITYRLAENKYSFVIPRPLDGGIIVGGTRQPEKLSPVPDVVTKKELIANAMKRFPELIVFRDGNATLDVKKVNVGFRPMRKGGVRLEKEFTENTQIVHCYGFGSSGFEMSWGAAELVSELVQRLPSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJA3</accession>
    <name>A0A1E3NJA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017303.1">
        <property type="nucleotide sequence ID" value="XM_019161089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJA3"/>
    <dbReference type="GeneID" id="30177776"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="134" mass="15383" checksum="77245D94FDBCD601" modified="2017-01-18" version="1">MIYFARVSDNHKSLAGIGELEEANVIRWSSFFNQDFRGNGISPMVGALTSGKPELEEASQNLLSYFDYLDNHLAVSKCFASNDNLTCVNISVCVFFKVIFSQLPEETIHLENYGNIYRWYFMLLQHPVVLKTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM6</accession>
    <name>A0A1E3NJM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_34561</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probably involved in the biogenesis of the COX complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SURF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016881.1">
        <property type="nucleotide sequence ID" value="XM_019162894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM6"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM6"/>
    <dbReference type="GeneID" id="30179581"/>
    <dbReference type="OrthoDB" id="10040024at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427:SF2">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="304" mass="34644" checksum="2D2124B5C4FD7945" modified="2017-01-18" version="1">MFSVVRSVRSNKKVTAQLQRSYASAKTANIDWNPVRSKKQLSQGIEFGGKTSSKVFLGLLCLAPIITFALGTWQVKRLKWKNKLVAECEDRLTYKPIPMPRNVTKEDLPNLEYRKVLVTGHFDYSREVYVGPRLHDTRRGYTVVCPFVQSNGAGEVLIDRGWIAAEKVIPSQRNLQHLSVPKGEITIECVIRVPPKKGIFSIDHDKGSRLYQYLDADAMAEELGSRPSLDYDTAEEFDPLQFINAGVPLGKIPKVDYKNNHLNYLVTWYSLSFASTVLLIYMFKKGKFMNPTEEKLKYTKKIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJS0</accession>
    <name>A0A1E3NJS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017474.1">
        <property type="nucleotide sequence ID" value="XM_019164197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJS0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJS0"/>
    <dbReference type="GeneID" id="30180884"/>
    <dbReference type="OrthoDB" id="160374at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052609">
        <property type="entry name" value="Ribosome_Biogenesis_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018849">
        <property type="entry name" value="Urb2/Npa2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682:SF2">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10441">
        <property type="entry name" value="Urb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal" evidence="1">
        <location>
            <begin position="950"/>
            <end position="1164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1165" mass="134560" checksum="F6113F2F9EFBFCEA" modified="2017-01-18" version="1">MVDVNKFQSTESITRFLRNKDTEVSDIYTFVTQELFSDSINSSIYLPQKERFVLELLVDRISQSNKLSQKFLYSDQTWKLLNHIWKIGKNDPRLTNIRSKVLTKLKFGDIFTKLLKELYSNNLYKDAPLIDDLILSLTHLVHSLKIHLSDDQNLTLIRYLLLLLENSNLYTSETIQRLLHLVFFIFKLNNTNSISYDTKHKSEFARTCLANTLIALQRHTTDSQVKSDLKYMLCKNIFSEDDKKATVTFISQFATVESNNKLNSDEVIFLLQLVIPKVGAQELESIVQILMSFYPLYSTQLLKHVTDMNKTLSTDFLSSMVEKELHRGKDISFDLIIHCIKRNSDVTFKYSEEICKLCSTGNPQSLMLFKEFFDSYVKSREVEIFIKRWSELVQAYPANIFDSDEMIDYASLTISTLSYTQLSKLFESEVESYTQNPNLIPIFLLSVCKGMMKAVSGSIQNALSKTLIQNMYQLKPQFSRLLKTDSDSSWKLKFFILSLFDREEFEDEVEALLAQPHSRNDDYFYATLRILEQDVSQARPKFLQKLISYFKKQSTKAFRKKIFCRWFRLLEVLLSKDEIKELIQKFMKHSTDQEIIEVLQIQLLDSQPNIMVEFVETFITSTSYLKFYQYISAYALSKKQKIQILDNLFEKLNDNEVPNAKQIMLNLIALPTFKSKLEAEFDSLLQLATYCDPDLNQLIRIILSNHLKQPVESAGYLDDAFATLTKQIDLLNKKKCVQKLQYLSLASILITESIGSSFEEKRVALASKLIGKIMDILSKTSDLTTDTLSSMINFLVTINHKSDNASIFPSNVKDIISSLGLKYPDDKSFKSLLFGLVCSIDKVYKPEYIFALYVVLSDDANIKYMELFVSRLAERESVFIESWLSICESMKSASDEDFEKYAELCAILLSNVKKSDDESKLRLTHVLFIQTISQIFTRTTRMASLTNACFLKSLKTVISTKIWLFTQYAMEMTMAFVSSISDKIEASKTLSTELVQDTYCDLCHLISSIVLYQRRRLSNRHHLIVSVLTSLMRSLFATSSKITSRGGLAFERLTSNLCEPNVSNLHVKRINDAAKDAEVSNALAQTKAGLRKHIPVLILAYIKYYLQYKVGVEVKPYLDTSSYMMLDLLTLNELNYINKSLDSQGRVVFKTLYENYKKFYKWNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJW3</accession>
    <name>A0A1E3NJW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_11431</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017545.1">
        <property type="nucleotide sequence ID" value="XM_019159792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJW3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJW3"/>
    <dbReference type="GeneID" id="30176479"/>
    <dbReference type="OrthoDB" id="4069986at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="100"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="102"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="180" mass="21159" checksum="41E7B836180122A4" modified="2017-01-18" version="1">MPMHQEELTDIYIHLKDILKTLSQTPVDREFYADGLINDMIKIKEILEENSIDEQSSLILSMINQISESISKILNLRYNLKTRTRIDFEDKIIPHTLSTQSEPNHGKRLPKETVVILNEWYADNIDSPYLSKDDISYLMSKTHLRESQIKNWASNKRRKKKEDYISPRVFQILSTNSPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJZ6</accession>
    <name>A0A1E3NJZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA exonuclease 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_28304</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">3' to 5' exoribonuclease required for proper 3' end maturation of MRP RNA and of the U5L snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017553.1">
        <property type="nucleotide sequence ID" value="XM_019162538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJZ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NJZ6"/>
    <dbReference type="GeneID" id="30179225"/>
    <dbReference type="OrthoDB" id="3996471at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF118">
        <property type="entry name" value="RNA EXONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="150"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="300" mass="33880" checksum="DEB80F4252587A0E" modified="2017-01-18" version="1" fragment="single">LQELPELCVPLQRLKRHGYVVEVPVAPSAEYTLPRTVDCSRCGVRFNVAEIAKEVSCSFHPGKLQTTTYNNLNMGKKVYGTDYTNRAHLCCSEIKGQSNGCKKSSHHAYKYNAAIDLHLSKPFQKINSLRKALNIKDDSRAQLLRREKIKAVGIDCEMCYTDKGFEMMKLSIVDFKTEKKLVDAIVHPDGDLIIDLNSHVSGVFEIPQNALTFDEAMVKLAQLTDEDTIIVGHGLENDLNVLRLIYPKIVDTAILFSENQVDVRRKDPLKKLAWSFLSENIQGREHDSLEDAIVPIRIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKE0</accession>
    <name>A0A1E3NKE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pre-rRNA-processing protein FHL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72647</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017702.1">
        <property type="nucleotide sequence ID" value="XM_019164261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKE0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKE0"/>
    <dbReference type="GeneID" id="30180948"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045178">
        <property type="entry name" value="Fhl1/FHA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712:SF29">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="152"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="394"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="3">
        <location>
            <begin position="394"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="311"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="517"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="680"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="934"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="476"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="517"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="573"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="699"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="943"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="967"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="990"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1054"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1088"/>
            <end position="1100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1100" mass="120329" checksum="F589E5E0E3A405C1" modified="2017-01-18" version="1">MKVQHSLELAPPQTQDPNTYGYTSGSDLNIQGKNTAFNGLNIEHFEMEELQKIKNDGVDLHPPTNLEINHILSEFPEGADMLDDRSKSSKTEEETETVHLQVSPNESLIKTKQQTDTVGKQNDEAIVDPQKIQAYAMLDFDSFTFYVQTMQILLGRMVEGDSLTDALDIHLGNQKAISRRHAKIFYNFGNQRFELSVLGRNGAFVDGNFVEKGVTVPLSDGTKIQIGETEFAFVLPNKERESVPVVAPPTDQIESLDSLVANFDEIKKEIQSDKLLMSKDPHVLNMVLDPDAQKLRELEIEKEIGKVLAREHPEDQNVAHLENTIDGELSQTNTKKKSTSKSKSGKGSDSSKDNDALQDPIKAESKAKAKPKRQPKAKKKVYTIDEIPEQYRTKPNLPYSILITDCLRQKGTEKGMSLSEIYKGIQELYPYYFYCPDGWQSSVRHNLSLNKSFRKISKEGKGWLWGVNEEVVAEKDRARQKQLENAKAKGKSSSPSALSPKLAQPPRQLQANLHIHNPTSIGSKNINANDLNNRSSDSLQYASPSSQNTNPSGSIPFAPAVSNKANEQQVVKAESASNNTSSTSHNNMSANTKKALAYLQKELISLTKSRKMYDRATSTEILTKALAMTISQVDQAAKNFSIKGFPLVILIDKNPGHVTKILTAALNAATLQVCKQKGLTPHLPPKNAPPTAQIPPNKVNANTDSATRATGSKSTPESTNKATTKVEPGTTNEFNKNGNVPILHIKKEGTETRDPIFHKVTPKVQSVIPPLATNSSKFTDEASVSSGSISVSNIGNISAIDSTATENNNSLTPPVQHTDAVGSTLQATTKQATGPVVYQISKAGMKPSFKPAVKPISGASVKSFGPSHPAIHQSSIKPIKPVRLGGISGTGIIKPQFYAKGKVPAIENARNAAVEGISEAEEIKRSIAKFGKPTANASVTVNDSEKKAELEAKEGVRDAEIDPVVSIQDSQKSTQPIIENNAPEQSVPSTDERPEPANKETEETTKESLPPDDKKIATLPPKKSKEDGEGDDELDDELHMMLADLEKDEDHDEPENKKRTIEEVREQADRKDAEGNGETNEEVEVEGGDEKRVKTDPTGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKF5</accession>
    <name>A0A1E3NKF5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017679.1">
        <property type="nucleotide sequence ID" value="XM_019161202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKF5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKF5"/>
    <dbReference type="GeneID" id="30177889"/>
    <dbReference type="OrthoDB" id="9991317at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001002">
        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001003">
        <property type="term" value="F:RNA polymerase III type 2 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1000" mass="116854" checksum="71CAD619839C8BE1" modified="2017-01-18" version="1">MTSPKHVSEDIEADIDLDDFSKDQLRQLLDDDDNYNEEALEFSDDGAEDSIAGDYNDEEDYESNDINSEDASDESDYEDSSNSKENRFKTLAELGFNEDEQEDEMISDNDSDEEFMFRNALREATNFKVKKSSKKTKKRGGLAALRRRIRRTELDPEVKMLLSKANEAFVNNDLDNALKLYSQVIKMDKNNFSAYKTLGEIYRMKDDLNKCSNIWLLAAHLNPKDVDFWKTVAEISVELGHFKQGIYCYRRAITASGGKDFDAIFSRACLYREIGKFKRASSGLLRLRSALPQDSRIVRELAKVYVEENRINDAISLYTKILDDNVKFRKLIDKGEQVTLDNIQFDWSELNILSELYASKAAWSLGIKALRQISRWIQERERQTFWDENTNNDIEFDERKFDHPRFQKLKSHEGSLAYTLPIDIRVQLGIFRLNNKNETEALRHFSILLEQPLEETPDLFFKVGMELESFALYKEALEFLIPLSYYEEYNTSELVVTIAKCLRETDDFENAKDTYIRLLEYDKDNVELKVALAECYFYLDNIEAANDLYKEAKAQRTKEKATARDNLVLNDTEADRVNYTEDEIQTMNVKSEIRVKKIYDKCLKLLEKIEILHLKESLSEAESSSLKTMVSIWIEMVSDLIEIFAMYRFFFSSDRAKKFNMRLRLRTAKLGIDQKLSRMKYLQHEIILSQNFSTMDYPEKEFKGLNYDQWYDLFVRYALLIAEFEKDTENAIGIQDIIKQVNVFKGKELTTQLVGLSIGILTQDTPLIMMQTRNLMNDYQFAIDSLRLYLASMVTTYEMEESFADPPSQKFMLRQIKAFDSLKDESKENVAGKATVTRKNVDITKDHFLINYIYASYLFTNKSYYSALIYLLKLYHTYPDNPDLLFLLALANLHRSVQRKTLNTNFQILQGMTFMLEYIKTKDQSNPYNVMEAYYNLGRSFNLLGLDHIAIGFYEKVLDIEVEDPAYDLKREAAHNLCTIYNLNKNFRQAELIMDQYLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKJ5</accession>
    <name>A0A1E3NKJ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_34010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017777.1">
        <property type="nucleotide sequence ID" value="XM_019162860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKJ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKJ5"/>
    <dbReference type="GeneID" id="30179547"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053239">
        <property type="entry name" value="Dual_spec_PTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="576"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="672"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="871" mass="99473" checksum="E8E6FCC240BAD294" modified="2017-01-18" version="1">MPIRSCNANGEITPNFASIITESTGLIKGTVSPGDVLISYSNIINLELYLQGILPNEVFKYYHPETIITDCIITQLIPVFKNLDPEIGINLRNFHIQVSKISNISDFIVYCFNREDHEFNVNQTEDTFVNNKCKCVSLSRLLHIAQIVYQKQHPELVKKKNLPNLLNNQKYNTFILADPNMEALNNADLLSIPVVDESASLKRDDELCSKYDISVFNNWDSNYLYRERLEISKMSTATPLSKSLWMGNITDYECLQIQLSNGKHIEVASTQEVQTMLKNQSESPLYCNAENTIVRLTKHDFEGVTNSEVSDKMLITFPLIVWKFYIKCVEGARIPTLDQLRLIYDNFSDCDFVNIEFPPSGSLSLADMSDDDILSIVNICKFCYYICDDNFPGLIYCSDGYTESSLLFLCYLMYSEDICLDEAILKLHMDYGRPFFIFKTDYILLSKLETIMNKFSPLKTPGETKEKFLFEENSKSIRSSLLMPKRKTSVGQVGSYSGTNNFGNTGIHNASSFVTSTGYHVVQYRGNQNIQSNQIRSNHSLFNQNNVSSTADRKKKVSAKVKPEIDGCFAEVHGSLPSRILKHLYLGSLTHASSIPLLSRLKIDYIVSVGERVSWCELFKHQKEVHKSGSEIITFVEGQVDENSGYELNVKKMLILPNINDDGIGTLITTIDHTLDFINDCYENNGRALVHCQVGVSRSATVCIGEVMKRLKLSLARAYMYVRVRRLNVIIQPNLKLMYELFKWEEYLLAKKPSKWKKINIGISSSNGCGVSAGFWTNTTKMKYIGSICEEPEQDFVIESEEEDEVDDNSQMVKLDFNSSIVKYEDSPRYKYVEEKEYDIVSNSRGSMREVDWYILCREIYNLNRAYIKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKS1</accession>
    <name>A0A1E3NKS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glycine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_32844</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017834.1">
        <property type="nucleotide sequence ID" value="XM_019162788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKS1"/>
    <dbReference type="GeneID" id="30179475"/>
    <dbReference type="OrthoDB" id="57698at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000158">
        <property type="entry name" value="Glycyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000002">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000010">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="199"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="652" mass="73699" checksum="CCEA7E8D9C9610DA" modified="2017-01-18" version="1">MSNEEFSREALESVLKRRFFFAPSFEIYGGVSGLYDYGPPGCALQANIIDAWRKHFVLEEDMLEVDCTMLTPHEVLKTSGHVDKFADWMCRDLKTGEIFRADHLVEEVIEARLKGDKEARGIAQEEPEEEDEQKKKRKKKVKEIKAIKLDDEVVQEYETILAKIDGYSGPELGALMKQYNIGNPATGEALEPPIEFNLMFETAIGPSGQLKGYLRPETAQGQFLNFNKLLDFNNERMPFASASIGKSFRNEISPRSGLLRVREFLMAEIEHFVDPENKAHANFDAIKDLKISLLPKEVQQAGKTDISEITIGEAVAKKIIDNETLGYFIGRIYLFLVKIGVDPKKLRFRQHMSNEMAHYAQDCWDAELQTSYGWIECVGCADRSAYDLSVHAAKTKEKLAVREALKEPQTVELYECEIEKKKFGPLFRKDAPKVEEWLNTRTQCDLEELAKELEQKCKITAKIANTAAAETEIPDSLIKIEKKKKTVHVREYTPNVIEPSFGIGRVIYAIFEHSFWTRPEDSARAVLSFPPLVAPTKVLVVPLMNNKELQDATKTVKATLRRAQIPFKVDDSGASIGKRYARNDELGTPFGITVDYDTIQDGSVTLRDRDSTSQKRGSLADVVAAVEAITYRGASWDEGTKKLVPFESKNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKX6</accession>
    <name>A0A1E3NKX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ataxin-10 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16604</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a role in the regulation of cytokinesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ataxin-10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017892.1">
        <property type="nucleotide sequence ID" value="XM_019161274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKX6"/>
    <dbReference type="GeneID" id="30177961"/>
    <dbReference type="OrthoDB" id="379794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051374">
        <property type="entry name" value="Ataxin-10/CTR86_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019156">
        <property type="entry name" value="Ataxin-10_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255:SF0">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09759">
        <property type="entry name" value="Atx10homo_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ataxin-10" evidence="6">
        <location>
            <begin position="424"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="542" mass="62592" checksum="C8C650859937ECD8" modified="2017-01-18" version="1">MATNMEVQLLEKLRAFFEDGFGNPNSLITDLGEVVQMSSLMMTNDNMWIYCVNLSEDEQLWKLLKALMIKVTGLRKLSIEQLRITKGIVLIVRNLLITHEKKAEMQSKSVDYNFFLEYFHLAYKIGKHLSIIFKSEEKDVFKLKTLDVSVVCFQCMFNILQCLSESSKKDLTEFSSTLDLLNDILVEMKSTGGVFEIKQIVLQFKEFCMSPEGRRVVADHDCLMLRNLITALTRSLKFAVDTDLANLKDTSKDDYNLVSTLAHTLIYLFNDEKIGSTMYRLERDGGEKCESMEVLLFLAACQMTFSIDASELESKWEWDFVAIGSMCLDFFHLYKERCISLLNLKTWNSNRERELSVVHRKMIAVLDIISNLLPYELFKKTLNSYDFLQEIIEFFKVVESNTERKRLKDSSELGLHKKSFPHAKIIIVEIVTYLVHGDFHNQEVVREHDGLQLVLTNCNLDVNEPFIRERCILCLKYLLENNQGNQAFIASLEAQRVEIDKEKEKVLEKCGYEVDIVNGKVQLKTHQGAETPGTEENKIAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKZ9</accession>
    <name>A0A1E3NKZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">guanosine-diphosphatase</fullName>
            <ecNumber evidence="5">3.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_33758</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.42" evidence="5"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017938.1">
        <property type="nucleotide sequence ID" value="XM_019162841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKZ9"/>
    <dbReference type="GeneID" id="30179528"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24040">
        <property type="entry name" value="ASKHA_NBD_GDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF83">
        <property type="entry name" value="GUANOSINE-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="158"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="437" mass="47982" checksum="D0DDFCED969D7110" modified="2017-01-18" version="1" fragment="single">DYEYVVMIDAGSTGSRVHVYSFDTSVSPPKLINEEFKMLKPGLSSYDTDAVGAAESLDPLLTIAMDTIPKDKHSCSPVAVKATAGLRLLGQAKSDAILKEVRRHLEKDFPFPVVSGNGISIMDGSDEGVYAWITTNYLLNNIGSAKKLDTAAVFDLGGGSTQIVFEPVSGEKVLEGEHKYNIEFGGRKFTLYQYSHLGYGLMQARNKVNAAVLQAEMKSAAHKFSALTESQLKTAVADVTLENPCVPPGVSAENVIVELEDGSKYVVNFAAPETEAKKDASLCKFLTDSILNKQLECASKSCSFNGIYQPSFADQFSSAADMYVFSYFYDRLQPIGMPESFTLKEMTDVMESVCAGSHLWDQYFKQQSHLKELADEPLWCLDLTFMTSLLHTGYDIPLTRELKTAKTIGGNELGWCLGASLPLLDKTNWSCKVTKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL79</accession>
    <name>A0A1E3NL79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_16687</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017994.1">
        <property type="nucleotide sequence ID" value="XM_019161308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL79"/>
    <dbReference type="STRING" id="763406.A0A1E3NL79"/>
    <dbReference type="GeneID" id="30177995"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000003">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="10">
        <location>
            <begin position="269"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="9">
        <location>
            <begin position="461"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="681"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="729"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="746" mass="82397" checksum="39DD3384EB960333" modified="2017-01-18" version="1">MAGPQTARGVEKPAKSGKIPRKGGKKNGQPSSATGSGAGTAKSESNRSGGGPAAAAGFVALKKSKKAKSKRKKSQLSDEKNSKADGKRKAGNEFENLDEEEEEEEKEQKEKAAAEDDDRLDSEDELVEISSASDSSGEEDADTSHEKGSGDPDRYISLVDSDSEDAGAGEEKDEGEEDQLPPLKRQKVKGSNMDDDYIAFDFSSDEGEAAAAAPGQADYSAYDDNEYLSNDEGSYHSKNIHHKAGEANDEFPWIRNSDHSREREMSDWLTSEIKDFVRYISPSAEEINNRNKVINNLKEHIRSIWPDAELHCFGSFATDLYLPGSDIDCVVISKRGRYDNKSSLYQLTSYIRNHNLGVEVVPIAKAKVPIIKFVDPQTRIHIDISFEKTNGLTAAKLIIDWLAQTPGLRELVLIVKHFLAVRKLNEVHVGGLGGFSIICLVYSFLKLHPRLSTNNIDPMENLGCLLIEFFELYGYNFGYDSVALAFNSNKEPIYLTKRSNPDLLGRNPFSLAIQDPYDPANNISRGSFNLKDIKRAFGGAFELLVNKCYELNAATYKQRLGKSILGGIIKYKGKQRDFLDARKHVKNVAFHAASGKVTKKTLTSTGGLPPLPSEKAGIDVDDYFMSDSYYSESESDTEKPAPKKSKKGGAADQYSKIFESSQKTQTVKKVIDKKPEELLDIKDDTDDAYTPELPVAAKKEPDFTDSDSDYDPSSTVPRNSDASVSAYRAAKEERREYWTQKSGGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLA5</accession>
    <name>A0A1E3NLA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="8">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_33437</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="8"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018038.1">
        <property type="nucleotide sequence ID" value="XM_019162825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLA5"/>
    <dbReference type="STRING" id="763406.A0A1E3NLA5"/>
    <dbReference type="GeneID" id="30179512"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002055">
        <property type="term" value="F:adenine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000004">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="14">
        <location>
            <begin position="46"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="193" mass="21645" checksum="650781E7BD712FD0" modified="2017-01-18" version="1">MHENEELQALAYELRCALHQYPNFPTEGILFEDFLPLFRSPQLFNKLIVAFKLYIEENFDQTIDYIVGLESRGFLFGPTLALALDAGFVPIRKAGKLPGEVYKVAYTKEYGQDFFEIQQDSFPEGANVLIVDDILATGGSAHAAGELVQKCKANILGFCFVMELDFLKGKDRLQAPTFTLLQGQPESLKEASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLL6</accession>
    <name>A0A1E3NLL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4-hydroxybenzoate polyprenyltransferase, mitochondrial</fullName>
            <shortName evidence="10">4-HB polyprenyltransferase</shortName>
            <ecNumber evidence="10">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">4-hydroxybenzoate hexaprenyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Para-hydroxybenzoate--polyprenyltransferase</fullName>
            <shortName evidence="10">PHB:PPT</shortName>
            <shortName evidence="10">PHB:polyprenyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">COQ2</name>
        <name evidence="11" type="ORF">PICMEDRAFT_59155</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>an all-trans-polyprenyl diphosphate + 4-hydroxybenzoate = a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:44504"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Mitochondrion inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
            <orientation evidence="2 10">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="10"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018079.1">
        <property type="nucleotide sequence ID" value="XM_019163347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLL6"/>
    <dbReference type="STRING" id="763406.A0A1E3NLL6"/>
    <dbReference type="GeneID" id="30180034"/>
    <dbReference type="OrthoDB" id="18170at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1780:FF:000001">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000003">
        <property type="entry name" value="4-hydroxybenzoate polyprenyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="325" mass="36683" checksum="1D4D0D1FB8800A62" modified="2017-01-18" version="1">MWNLADAKGVFSAEELEQAKLQRLNGLGILKNFPDKWVPYMELMRIEKPIGTLLLLSPSMWAITMAGYMSAAPLLSTVWMLSVFGVGAFVMRGAGCTINDMWDRNLDNKVARTIERPITSGRVSMKQAWVFLSAQMLVGLGVLLQLPLDCFLLGASSLLLVSTYPFFKRVTYYPQVVLSACFNWGALLGFPAMGVWNWPVMIPLYLSGFFWCMHYDTIYAHQDKKFDIKAGIKSTALAWGTKSKTIFNVLSTAQMSCFLTSGYLAGMGPFFYLGAAVGAYRLFSMTKKVDLDNPANCWYWFKENINTGHIFWLGILLDYLWKIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLQ4</accession>
    <name>A0A1E3NLQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71196</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018188.1">
        <property type="nucleotide sequence ID" value="XM_019163857.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NLQ4"/>
    <dbReference type="GeneID" id="30180544"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF119">
        <property type="entry name" value="ROA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="510"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="655"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1074"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1150"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1188"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1215"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1301"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="86"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="732"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1337" mass="151431" checksum="526D13E4DE991F81" modified="2017-01-18" version="1">MASIEKELLHTTLKSSTLDSNTGSPAESNHDYNDKDENNYLQYYEENISFTRTKKVKITVRDLSIGSLFSGSQRSLANVLKPFSKKNPIDIESAESIKTILHPMSFDIPSNSLTCIVGGSGSGKTTLLNRLANRKFSKKAMFTQGVVKYNDQDDLSSYRHAYVIQQDILIPTLTCSEMLMYSAELKLPKLSSKEQRKLLVDEIILTLGLKECKDTLIGDKKHKGLSGGEKRRLSVGLQLISNPSVLFLDEPTTGLDNYNAYLLCKSFQHIAKRLNKTIIMSIHQPRADIFKLFDTVLILSKGKLCYGDSYSKIFSHFDSLGYRIPETKVNPADFFIDITSIDTRTKAQEVESYERVESISNVWKEKMKSFQAICDTDLQSEDIVQESELFQAVGRAPFWREVNILIRRNLKLQYRDPVGWFSIVFEGLFLGLLCGWLFFKPGTSVAGMRSIESSLYSTCSLQCYLFLLYEIYRLCNLDLKVFDRERMENCVSVSGFLLARRVSKLITEDFWISLFFSLTTYFMYGLRLDSAKYFFKYFAGCLIFHLNTMTFATFAASLSRDVSIATLIGNLNFTFQSMTNGLFANAKQLPVYVRWCKYVAYLWYSFGYLASNQFSNYRSECYTINADNPDVNQICYQYSGKYILNNLGFWENWDALPICVVLAFAIGHYVAAGLILYIKPLDMTMGKEVKSSDSIDNSIDYSQMVEDIKHQRQQNALISTENGDVDSITLVVKNINLSVTNKLKKSSKEILHNVSAVFHSKKLNVIMGPSGSGKTSFLNLISGRLSSNLMTQYTSSGDIYLNNYQIEDYHMIRHVCSYVVQEDDHLLSALTVRETLRYAARLRLSKSSYTLSEKHTVVDDIILKMGLKDCANTLVGNDLIKGISGGEKRRLSIAIQLISSPKILFLDEPTSGLDSFTAASIVECLENLTKQGTTIIMTIHQPRTLDHFGSVLLLAKGGKVAFDGKEDKIIPYFDSIGYSVPHLTSLADFVIDLISYNTSNKEIEEQTRARVTHIINSWAAVTDIQIPKNALILNSKSQVRETFKSTIKEPASFVIGFWVLTSRQWVGLVRDKNILIARTTQILGMAIILALFFSRLKHNTTSIQDRLGLIQQMTALYFTGMLNNLAAYPQERDYFYDEYNDDVVGLNSFFFSYFFIEIPFEVVSCFVFTIFMVFVIGFQTDAKLFFTIFYTTFLIVNAGESLGISFNTVFEHPGFALNVISVFCSIGVAMAGLLAMTLDGFLKALNYTSPLHYCVMLISNQVFTSSLRLTCTANERLSNGECLFNSGKQVLDQYNLKVDMTLYYVLISVVTVLQRVISWVFLRVKLMKISTKKLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLX6</accession>
    <name>A0A1E3NLX6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_31487</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018240.1">
        <property type="nucleotide sequence ID" value="XM_019162703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NLX6"/>
    <dbReference type="GeneID" id="30179390"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="695" mass="77463" checksum="F7A96496F18E75A8" modified="2017-01-18" version="1">MSTPFGVDLGNNNSVIAVARNRGIDVVVNEVSNRATPSVVGFGMKNRAIGETGKNQQTSNLKNTVTGLKRLLGLSTESPDLQVEQAFSTVPLVGKEGQACAKVRFQGEQQEFTSVQLVAMYLNKLKDNTERETKSTVKDICLAVPVWYTEAQRRAAADACKIAGLNPVRIVNEVTAAAVGYGVFKTDLPESDPKKVAFIDIGHSSYTCTVAAIKMGEMKVLGTGYDKHFGGRDFDKAITEHFADEFKEKYKIDIRSNAKAYSRVLAASEKLKKVLSANSAAPINIESVMNDVDVSSQMTREDLEELVQPLLKRAANPVHEALKMAGLTTEEVDSIELIGGTSRIPCLKNTITEIFGKPFSTTLNQDESIAKGAAFICAMHSPTLRVKPFKFEDINLHSVTYFWEQGSEDTDHLEVFQKGASYPSTKIITLYRTKDFEVDAKYTHPEDLPPATNTLIAKWSVSGVEVPENEESATCKLKVRQDPSGFYTIEESYIVAEKEFKEIVEQPEPEKKEEAAEGEEGEEEEAAKEPEYKIVKKLVKVKDLNVQYFGPTLTKTILDEFHEKEFKMIADDKLVAETEERKNALEEYIYTLRGKLEEEYKDFASPEEKEKLSKMLMDTEEWLYDEGYDSTKGKYVAKYEELASIGNLIKGRYNAKQAELKQKKNEKKEAAAAADIFAKIAANRKKDGDGDSEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NME2</accession>
    <name>A0A1E3NME2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA-processing protein EFG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">rRNA-processing protein efg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_11751</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EFG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017891.1">
        <property type="nucleotide sequence ID" value="XM_019159836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NME2"/>
    <dbReference type="STRING" id="763406.A0A1E3NME2"/>
    <dbReference type="GeneID" id="30176523"/>
    <dbReference type="OrthoDB" id="47732at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019310">
        <property type="entry name" value="Efg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050786">
        <property type="entry name" value="EFG1_rRNA-proc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10153">
        <property type="entry name" value="Efg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="199"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="249" mass="28544" checksum="92139F90EE05E8EA" modified="2017-01-18" version="1">MAKYKETSTAVGVDLTRALAHGSSGIRKKMRDITRLLQRDNLAADIRIANERALKTLKLELQKVQGDQKERKFAQKYHKVRFFERKKAIRFYIKSKKSVDALNKQIAEIEANGDKEAEKELKKQLKKEKRVFGHSQVDLAYVLNFPKNEKYIALYATNTTPVEKLPKKAQAGIKASNERKEEFKKLFADQLKEGTLELGLEEGLRKEGSEETAENSNRVRTSANHVNKKSEQIDDVEAAAENEKDDFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMG3</accession>
    <name>A0A1E3NMG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 10">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1 10">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 10">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15227</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1 10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018359.1">
        <property type="nucleotide sequence ID" value="XM_019160724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMG3"/>
    <dbReference type="GeneID" id="30177411"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070184">
        <property type="term" value="P:mitochondrial tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="501" mass="57116" checksum="BCDA9460600ABAD2" modified="2017-01-18" version="1">MLKPRFFFVAQKRLFSESFTRSSFHVSSSISKLLDKHSIEINESDADHDFKEPLLQHLQKRGLVETCVNEKHLLDHAETKTLGLYCGADPTAKSLHLGNLLPLMVLLHFNLRGHNIYPLIGGATGEVGDPSGRTTERTAMENNTRLDHVRRISDQFLSFFNTATLYGETRNPLIKISEVGSRELKNNREWWKDMGMLEFLAKYGRHIRVNQMLSRESIKNRLNSEQGIGFNEFTYQILQAYDFYYLNKKYQVDIQVGGNDQYGNIVAGIDLINRLRKAEESKSSREIYGITVPLLTTSNGVKFGKSAGNAIFIDKELTSSYDIYQFMFNTTDEDIPKFLYKFSLLPTKVIDKIVHAHNSDKRSRFGQKVLAIEMCDLVHGDGEGFSNYVISEILFSKVNIKENFKAVEILSAFKKQNLVTQISKEELTQLNIVNLLHKVTEGEKSKSELKRLIKGGSVYIGNTKEGKVTEADYIVNIITDTIEEKLLLLKIGKKYFVVEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMU9</accession>
    <name>A0A1E3NMU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_71478</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Component of the lid subcomplex of the 19S RC.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018519.1">
        <property type="nucleotide sequence ID" value="XM_019163936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMU9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMU9"/>
    <dbReference type="GeneID" id="30180623"/>
    <dbReference type="OrthoDB" id="1452at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000005">
        <property type="entry name" value="26S proteasome regulatory subunit N7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049549">
        <property type="entry name" value="RPN7_PSMD6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21154">
        <property type="entry name" value="RPN7_PSMD6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="197"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="403" mass="46158" checksum="59F70EADC1C4F4AA" modified="2017-01-18" version="1">MAITTDDIETKEVPNFRLSETVFLAQHQQEHPGNEKLIGEILETIRKDSMGPYYHYLYYELKLPGFEWDQSLYDSLVAESSKHIAEITAEIEKLDKEDESEMDILKKWTELGEYYATIGDKTKAESTLLKTIELAPSTGSKIDLYLMISRVGFFYNDAVFVKTYLDKSNALIEKGGDWERRNRYKTYNGIYLMSIRNFAEASKLLNDSLSTFTSTELTTYQDVAKYALVCGAIIFERPDLKHKLIENPEILAINSTTDELLPIYNLIKSIYLTEYEKFFPALLETNDKVLLTNKYLYAHANYYLREIRCRAYSQLLESYRSLSLKSMAASFGVSVEFLDNDLCKFITSKKLNCVIDRVGGIVQTSRSDSKNAQYHTLIKTGDALLTKLQKYGAAVRMSGAEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ8</accession>
    <name>A0A1E3NMZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_10481</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018600.1">
        <property type="nucleotide sequence ID" value="XM_019159676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ8"/>
    <dbReference type="GeneID" id="30176363"/>
    <dbReference type="OrthoDB" id="3997888at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="652" mass="72304" checksum="FF4C152BC10BE190" modified="2017-01-18" version="1">MEESAGGVEDENIFAFGTQVQAGLDDFKHEIQVVDFWKDKLNGYAHQEKSTVEPKKDKVSKPICVKKIRRPKNSNKKQHNGIKDKNANRQRKFGSINKEIIRKYKALQMSHGVDVQAYNNFADEEMKMLIGKEDWDSIHGLLMECLKNDETDNIVKSDKEKIVTEYTDFRQLQESNSTAFAAAGAAREVSADVTGVNGRGTADAKLTQYSWSYPVDFTPDDLGALYDITMSGIDLFNRDGNKTSEEAVEKDSTSLVEELNNTANQTSGVFTLSQVLGENTQRSLDVSQREKAKAEEQIQELDCVIVEDSCDSDGAEDEITFDYIEKIREEKVESGTQQLPIELDSSNIIEIPNSSDDGGYDHGDFLDDIGSYNLRNTEGDIIEIANSSTCGESDSDILLTGCPESGTQPLMDLNNLQRESELITDDAVGSYERMIVPTSSPASSAAGSQEINVNEDNVLTRSVTEIIGRESESLVDNCAAALAEVPGELVTEEERVSLDTGVDIAKRMKHFEGWNVVMLKKQLSEWGVKNASKMGKKKLEEALANICKNVTRDKWDWAIDKSQTGEILKFAEWESEHPEDVEGAAGTEAVIEKAVMGQIKALLKADAGLYYDVLRYKPLNVHMLLGKLAETSDRKVVCDCLDRLGVCWTEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN27</accession>
    <name>A0A1E3NN27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_32732</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018161.1">
        <property type="nucleotide sequence ID" value="XM_019162785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN27"/>
    <dbReference type="STRING" id="763406.A0A1E3NN27"/>
    <dbReference type="GeneID" id="30179472"/>
    <dbReference type="OrthoDB" id="438939at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140727">
        <property type="term" value="P:siRNA-mediated pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038737">
        <property type="entry name" value="SF3b_su1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12097">
        <property type="entry name" value="SPLICING FACTOR 3B, SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="9">
        <location>
            <begin position="866"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1097" mass="123449" checksum="EC46A4D1E2807152" modified="2017-01-18" version="1">MTSKESEKTQLVGAYSVPKDIVDGITEEAESAAEYEIGAGRIKNGNSSSNNTAKRRKKRKFELIQEKMARTDNSKTPESDVVHHKREMERRRKREIEADSAADENGNKTPPRTERRGRKRRLESEENGKVADEKEKDKKQLKHVSRETITKGPSCPVVMGIPLTTQNLDKLLPSGFSVVPFPADYKPLNNLPPDFSRFESFAAPHNALLATVSKEVVGRENLDRKQIYEIPDLKDLQYFSELDMKVFHKLIAAKNSKQGSLSNHEQLELRCMKLILKIKNCSSASRKSALRNLTDNAKLFGPDIIFSIILPLMSDPALDEQGRHLLVKVIGRVLFKLEDSIKPYAEKILIVIMPLLTDENKIARLEGRDVISKLSKAVGLLTMITILRPDLDNSDEYARALVSRAFAVVANSLGISALIPFLRAVCRSKKSWLIRHTGTKIIQQIAIMIGSGILPYLNQLIQCLSKNLDDEMLNVRTITAATVSSLAEASAPYGYESFEVMIEPLWRGLKNHRGKALAQFIKGLGNLIPLMGEENANFYSYELLKIVRRESTTSDDEMKRAVLITIEKICALKTIDNELILKSNISESFFKNFWSRRVALDKKITDLCINACYALSLKIGTLEVILAVLPSLKDESEPFRKMSLEASEKVLTSLGCFDLDDKTVNRLLDGLLYCFQHQHLENKQTNSIILNGFGNILNNLGIRVKPHIMSIVSAILYRLKNKDAEVREQAADLISKIVDVLKVCEEEDLLIRLCTILYESLGEVYPNVLGSILCALRSVILAIKSVDSLNPSISQILSTLTPILRNRHEKVQEMAIPLIGDIADRAKDYISHREWMRISFELLEMLKAYRKSIRKSANKTFGLIAKAIGPADVLVTLLNNFRVQERQLRVCTAVAIGIVAEVCLPFSVLPALMNEYRYPDKNVQNGVLKAIGFMFEYIGELGSDYIYATTPLLLDALTDRDLVHRQIASNVVKHMALGSFGQGYEDAFVNFLDLIWPNVFETSPHVISQVFESIESLNIVLGCGILLDYIWTGLFHPARKVRESYWKIYNNVYLSHVHAMVPYFPRFEEIGDEPGASKQEAALLTGDLGVQELDLWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN95</accession>
    <name>A0A1E3NN95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018693.1">
        <property type="nucleotide sequence ID" value="XM_019160859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN95"/>
    <dbReference type="GeneID" id="30177546"/>
    <dbReference type="OrthoDB" id="3998230at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="366" mass="41455" checksum="CCFE86F00907336E" modified="2017-01-18" version="1">MHNPNELLNRFGNKGGNTRSPSPTRKTISSSNQFRNRMRPHAAPFDKSAKTPKLMPLQDSFRSKEMKDVAFEVTVDIDDNAQNLHPLDEPFILRNGKPIYPSDASPTRLNKAARTLSNDIKMEDVISLTKSVWNLAHSLLLAFWNIDLYFNVTISENSTMKVHIPTLVFSLVVLRLFFSISTSGPHETYSAPVKHSSSSSSSFVISTLVLLSAAAYWYNYCHRSSDNSTFVTSPSSSISEAATLTDIIDKAAAIPDIESTSYSDSDTLVSSTKSQPNTIWCDERPIAEHFDDKTIRLETILNRNKVPIEKDFHPLHRQEHKQRHPRSKLKLNLPLDNIKRKTDDPDHGFQEMTKIRRQEMLKAFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNC3</accession>
    <name>A0A1E3NNC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hap4 transcription factor heteromerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_107895</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018261.1">
        <property type="nucleotide sequence ID" value="XM_019159714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNC3"/>
    <dbReference type="GeneID" id="30176401"/>
    <dbReference type="OrthoDB" id="5374328at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018287">
        <property type="entry name" value="Hap4_TF_heteromerisation"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10297">
        <property type="entry name" value="Hap4_Hap_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hap4 transcription factor heteromerisation" evidence="3">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="452"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="719" mass="77144" checksum="EFF5525FD4C701F7" modified="2017-01-18" version="1">MASADPPATTGPTSQTAQTAQTAQIDRKVPSAPDRKQTAPTHIPTSAFTPVNDLPLEIKEPIKITTSKKWVLPPRPKPGRKPVSSSDLDTAAFSLDKDSSKSKICKPDIHHNHSHNHNHLRQVTIEKRPFSFPPERDNSISTAVPSGNTTLASSPADFSCLSPKSSSSCMPTPSPTPDSLSPKLESNVPVAKPPQTQLQPPAHTQPEKRKRSVAKLAHHAAAGSKRKQDQPVVTNSNKMIEVDCSIIHNPLKTEILKINEENYYLKLEVIRLVSNLKGLRDEIQPIVDKRKTKAGRKVKKEKSTAAAKPAASSALPTAAPTTVAQGVQKQNPNTFTPVLQQASSSTAPAATPVTAPTKKRNHDDDINDLILSLIDLSHSQQTSESKPASDEAAPNSDSARNTNASTTLTMTPTTTTETNTHSHTHTDTNKYTNTNADMKRSPETKGTNNKATKMKPKTKLKAKPKPKPSNADLQADAAPIPDAVDFATMNAPSSSMTTAAAATVTAAPPATSTALSPLVLDQSADALRQGQHSHVQSPVFSNDLVNDPFFELSSKALLDRQTLYDEDDLDLLSTVSTTPSTMFSLSLSATNETVDSVAGSSGPMLMGGLENIDELPPFNLLNLPDEKAIAAGKLDIRLNYDYDRSVAENRYPPLDTFSLLDGGISGDRDRDIGSLQLLSDRRLSVDGSSGPNTGELVDFAMPEDVESVFDTFIHGRGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NND9</accession>
    <name>A0A1E3NND9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_116594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018724.1">
        <property type="nucleotide sequence ID" value="XM_019159820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NND9"/>
    <dbReference type="GeneID" id="30176507"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="111" mass="11714" checksum="45E9AB230AB930A1" modified="2017-01-18" version="1">MSVLPRSPETGAAAACLLPACLSSCLPVCSPFSISVRASLHTPSTRPRYGAIAPQTPRPPPPPLPLAGKQKQNVARLSTFLVCLLRLGRAQRFGGTAESHQDGTRGHRASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNT0</accession>
    <name>A0A1E3NNT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Stress-associated endoplasmic reticulum protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Interacts with target proteins during translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation and facilitate correct glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the RAMP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018852.1">
        <property type="nucleotide sequence ID" value="XM_019160908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNT0"/>
    <dbReference type="GeneID" id="30177595"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010580">
        <property type="entry name" value="ER_stress-assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06624">
        <property type="entry name" value="RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="65" mass="7583" checksum="F80C078C43F7B24F" modified="2017-01-18" version="1">MAVQSFHQRRANEKFASNIKKVRTGEKQKQKKEGIEKVNIPRWLVILLCFLLLGGGILEILRIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNW0</accession>
    <name>A0A1E3NNW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SGF29 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71807</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018888.1">
        <property type="nucleotide sequence ID" value="XM_019164013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNW0"/>
    <dbReference type="STRING" id="763406.A0A1E3NNW0"/>
    <dbReference type="GeneID" id="30180700"/>
    <dbReference type="OrthoDB" id="10265994at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20393">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20394">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037802">
        <property type="entry name" value="SGF29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010750">
        <property type="entry name" value="SGF29_tudor-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047288">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047287">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539:SF0">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07039">
        <property type="entry name" value="SGF29_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51518">
        <property type="entry name" value="SGF29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SGF29 C-terminal" evidence="6">
        <location>
            <begin position="256"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="398" mass="43002" checksum="361BC16DE351D9D7" modified="2017-01-18" version="1">MSWDNIVLSLQETSNTLLAENTKAISTKAKPKAKNKVKSKNIDDDSTNGLNQISLDVLLKVSKQLTTTLEDVEMFLKEKDVSFNTTSLATDESIHDLIPSNEADDIDNENLAFEGDLTAGRTAISSKEMSANSTSTNTTMTYQSGLNAKGRGRGRPQGSTNSRLQGSGVSASAVSSKSFSAHSNNTASTNAEADEGSDNTAVNSTNSVGNSSSATGAAVSDKLITIGARYGYPPPPPLSSYNGKIGRSFYTSAYNPSTPILPNSQVAYCPSNGRDGANNFATMHSEWILCRVLRVISETRFEIQDPEPDEMHPQGQVFRANYKEIILVPLHLVSSSISKLKEYKSGTRVLAKYPETTTFYPAEVMASKHGNCMLRFEGEEEVGKLTPVDRVFVLPCPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP91</accession>
    <name>A0A1E3NP91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Mevalonate kinase</fullName>
            <shortName evidence="19">MK</shortName>
            <ecNumber evidence="3 19">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PICMEDRAFT_32191</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Mevalonate kinase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17066"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="18 19">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 19"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019034.1">
        <property type="nucleotide sequence ID" value="XM_019162749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP91"/>
    <dbReference type="STRING" id="763406.A0A1E3NP91"/>
    <dbReference type="GeneID" id="30179436"/>
    <dbReference type="OrthoDB" id="1652964at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000003">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 19" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 19" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="16 19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="15 19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="20">
        <location>
            <begin position="125"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="21">
        <location>
            <begin position="296"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ47921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="427" mass="46902" checksum="B86EA12FA2BD9FE1" modified="2017-01-18" version="1">MVNYPFLTSAPGKVILFGEHSAVYNKPAIAAALSLRTYLLVSKNEDDPEGYIRLEFPDIGLSHQWMKSEMPWDLSTHEQGRPPAIEELNAELVNGLTPMLTTITSPLHYAAAYCFLYLFVSLCHGEVEHGLTFTVRSTLPIGAGLGSSASISVCLASAFAYIGGHIKAAGLERDSKIVKDSDECLFIDAWSYMGEKCIHGNPSGIDNAVATHGGAVMFQRMENAVPNVRTSMRNLPSINLLLTNTKTPRRTADLVSNVARIVNDFPKSTGCILESMEAIAREAYNLMLRPFFDEDSKARLMELMRINHGLLVSLGVSHPNLERVKLYCDELKMGETKLTGAGGGGCAITLLKDDVDAETYEKLFAKFEQNGFETFRTTLGGKGVGLLEDPAEALLQYMTPESFVNFNNTTEIETSIGCTAVESWKYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPA3</accession>
    <name>A0A1E3NPA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019020.1">
        <property type="nucleotide sequence ID" value="XM_019160964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPA3"/>
    <dbReference type="GeneID" id="30177651"/>
    <dbReference type="OrthoDB" id="10563174at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="623" mass="72143" checksum="6D13CC468AB2950D" modified="2017-01-18" version="1">MFIRKTPTRTCRYICQYVNFRRNESTAVLVPLQKNPTYNDTHRKDGVQTNVVEDGKDGLMEGYLSINTSAFSSLSNQISNQERIFQDSSFQTLSMSDHGSLVQDIQKESGSSKGFLDDVFSGGGYSVDEEELNVNFRTYESRGNEKTKRHPVNSIKRAMSKNPNERKLLKLASAFNDYKIAESSIDEFYKCVVGDDNVIATYDFKMPIFPSELDNTSLKKYLKLVNSIDFSYDSKGYERLVFYDMYGLVYNIHKFEGIVDREIMHLFIKYFCNYSMIRTVFRVIDKFEKVGITPNRNTLHILIYKLGKISNLNSRKDLLQLYLIMGMKKWKVSTDLTTRALMYSVEKPSRKRLALAKALRNEGVDHSSMKWEMCRDYVTIEVNSRPKTSFSRMAGHIKQLANLGNSNEDMCKVFQIYIDTITHRNKTGTAFHEILAHPHYEGVANWETIVRQLLEANDIWGCLAVVNLLANRKGTDLRRVIFPLLHNYKQLYAFFTKQKFKKHHEKHLFCSVMTLLQELCHTKMTQAAAFVGSVQLEDNIVVGKIDREVREEFKQIRLWDGKCRDFSCFSPIAGSHVGRTDIMRLWDAYVLEGERESMGMLSVDPLSFPWRLKKNPPFIECIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPL1</accession>
    <name>A0A1E3NPL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019157.1">
        <property type="nucleotide sequence ID" value="XM_019161005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPL1"/>
    <dbReference type="GeneID" id="30177692"/>
    <dbReference type="OrthoDB" id="3996121at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="525" mass="59345" checksum="026C24FE42109209" modified="2017-01-18" version="1">MYNKKLRGDFIDPEIDFRGSVANRSKLSICTLPPINTIVGEGPKSTHAKVFAEQYIQAKHSIDQRTPVPVVAPQKTQSISLAKRRGDKSSRIASGNIFSGWSKRISTFGNIMESFEVKEAKQRSVSLPQYHATTNIKNSVHFPPQLESHPHPQPELLPHPRPQQKLLRPPNPDHGIIVNAQQTINKSHKHRQKPRYKSSSIKDNHQCHRKKVSFGNNNFTNHKPFVPVHRDTSQVTGNKLTECHPIGGLKNQLENRYVKVEDDLLTNFSNDSSTSLVSKLLESYSRMNLEQQSFEKAKMPDSPGAKALQLNPVEEQKATKRSMTGGTSTHDSTSTNNDLFSFTHSSYTSGSSEFVSLPETGNEDGNSYPNALQTTNYNKELPPVPLKKMDQSILRDYSGAEQDPKIVDSSVPPEVPRHQILPSSNSQHESFELFYDCDTFSFTSSKKSLERNDTKIMERKPEKKEYFPEDTAIFTRKSRQLKGKKKPVPNSKDRTTSWQYLEKVGIEPFMLSGYQSDTPLKVINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPL2</accession>
    <name>A0A1E3NPL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin efflux carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_26921</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019174.1">
        <property type="nucleotide sequence ID" value="XM_019162470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPL2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPL2"/>
    <dbReference type="GeneID" id="30179157"/>
    <dbReference type="OrthoDB" id="2499604at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794:SF2">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="45194" checksum="41E1C3FB4724634F" modified="2017-01-18" version="1" fragment="single">ARNGLSFQEISFLTFQAVVQVSLSLKKLMEVIIIPILYAVTTLVSYYSSILVSRWLELTEPESNFVTAMSVFGNSNSLPVSLTLALAYSLPDLEWDDIPDDTPDKIASRGLIYLLIFQQLGQMLRWSWGYNKLLAKQPDHVIFYGDQPNSKTTSSSSNTNVNVNYLTANYGNGEGDDDDECGDGDSTTQLIEEYPSTTSGAMSSSTSYGNNNQHGEDSEDKEVERPFLVRVLLGFYNWMNPPLWSMLAAMVVASIPPLKAAFYENNGFVQHTLASAIKQLGGVSIPLILVVLGANLAPSSDIPPACPHYKRMILGSLLGRMVLPSLILLPMIVLSVKYISLSIMEDPIFLITSFLLITSPPAIQLSQICQLNELYQKEMAGVLFYGYAILTLPVTIVVVVTCIEAMKWSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPM8</accession>
    <name>A0A1E3NPM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type 1 phosphatases regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15907</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Regulator of type 1 phosphatases which maintains protein phosphatase activity under strict control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the YPI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019177.1">
        <property type="nucleotide sequence ID" value="XM_019161017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NPM8"/>
    <dbReference type="GeneID" id="30177704"/>
    <dbReference type="OrthoDB" id="307488at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900180">
        <property type="term" value="P:regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="156" mass="17439" checksum="DE4645230AF63F2C" modified="2017-01-18" version="1">MPTGAPSGTRTQTQTIQIPDIPTLHLRASATPEQESEENTNSNKKTKKKVSWEEDVVDNENMGKKKTKICCIFHPQDEDAYEVEDGSSSSSSSSSSSSEDEGEIKDRAEPNAYERQPKYKKKKKLSKESSSDNHHSHSHGPDNHAHFESHSHTHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPP5</accession>
    <name>A0A1E3NPP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase delta subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_10924</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019109.1">
        <property type="nucleotide sequence ID" value="XM_019159729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPP5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPP5"/>
    <dbReference type="GeneID" id="30176416"/>
    <dbReference type="OrthoDB" id="3995692at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.20">
        <property type="entry name" value="DNA polymerase delta, p66 (Cdc27) subunit, wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019038">
        <property type="entry name" value="POLD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041913">
        <property type="entry name" value="POLD3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598:SF13">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09507">
        <property type="entry name" value="CDC27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="438" mass="49426" checksum="C0C308A5FD81563F" modified="2017-01-18" version="1">MTQEHIDYLEKRLGISKKPVTARFLSRKWMVPAKEASEIMHHFYTSHQTDSVFKDLSIKYSVCGKQKAVKKMDKEENFERHADVLVKIVDKDDLNDSIEKLFSSVVSCQMYSMHLQSAVSLTNIIDTCTTNKWSFSEENMTKCGILQTATNGILEQPEEQQEDRPARRAQTEPLKAQNSIHIEDKSESKTQDKTESKTKAKMEDHDKSRPVYVSRKASSNPTPSTSASTKISTEARKPLYTSRKRNKEPAEVGNKDISSATSSKRAKLSSQAERKKQEDEKKGLAKMLEDDFSDDAFENVNQGDDDSTVEKETEYKYDDIDIAEPEEEKSNEAQGKTMHESQEETEATLENISEDDVAENPATISSNQPAEPQPDVETYVDADGYVVRKVNRKTAKPAQSSTTPAKSSYTIDTKPLKNKGGNSARKQSNLMSFFKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX0</accession>
    <name>A0A1E3NPX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ERCC1-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_24548</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019262.1">
        <property type="nucleotide sequence ID" value="XM_019162356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPX0"/>
    <dbReference type="GeneID" id="30179043"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22325">
        <property type="entry name" value="ERCC1_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="7">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="207" mass="23626" checksum="29F4A1A597F5FD13" modified="2017-01-18" version="1" fragment="single">NQIQVSRSQAGNPLLEHLTFYQTNSKIKDVDYVINSKCVALFLSLRYHKLHPEYIYNKLKKVSYNHEDTLRVLLVYVDIDDFQDTVRELNKLCLFNKLTLILAWTNEQCANYLQNLKYVEKEASKRIIQGSRTKDEDVLANDGKYLERVVNTVSSVKSVSQSDAKSLLVAFGSMRGIVEADTDQLSGIQGLGQLKVDRLLRAFNGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX7</accession>
    <name>A0A1E3NPX7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15666</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018881.1">
        <property type="nucleotide sequence ID" value="XM_019160914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX7"/>
    <dbReference type="GeneID" id="30177601"/>
    <dbReference type="OrthoDB" id="10425070at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="212" mass="24753" checksum="35FC7314F1DE4A95" modified="2017-01-18" version="1">MEEEEKDAADEDEVQEEEEEEEEEEEEEEEEKEDEEEGDHASNSMTLMGLYTEYEKSVKLEENKIRNLEDSIFLGNLVEHYVGLLGMIQNVEDEFAKRIKKGELSRSNVISNIRRGMKRNMVEEGRIQKVSNDDGSAYLNKKYQKLYIAHSLLENDYDELYEKYESLKIDHEDLQSLQNMPKTGPESNMTRAGFKGKGFKIGRKLVSYNSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQI1</accession>
    <name>A0A1E3NQI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_8909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019473.1">
        <property type="nucleotide sequence ID" value="XM_019165036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQI1"/>
    <dbReference type="STRING" id="763406.A0A1E3NQI1"/>
    <dbReference type="GeneID" id="30181723"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF23">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE ICT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="143"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="548" mass="61311" checksum="32E668E3574D6469" modified="2017-01-18" version="1">MALMTTATATATALTIVIGRETIAQAAIGCRARRYATAKKKPSVSYDSGILNSFKIWYREMSNYDDGDAFAQDQFLSYVLPYYRPKELVEDKDRAMRAFSVKTPLRKREEPDLSDSNQWYLNEIRVEKRETRGTSEKPAETKHLVMLHGYGASSGWFYKNYKGIIEGSKRVGNLSIHGLDMIGFGLSGRPNVRFAHDADTKPSLEIETQGIRWGRYAVCLKCGGHLDGKHSTGGHWCSCAAEEEDLRRGVAAGGGGPASVVVKKEDVYEYVQNQRALVAEAEDVYVESLERWRAANGIEQFDLVAHSLGGYLAVCYVLRYPGRVGRVVLASPGGVERTPFAVSNPDYARLGPHDRGTLRMPVSNRVDEYGFLGRYGLTGSVFRLLWRMRVSVFTFLRWLGPLGPSVLIGRNAAKLARSGNIQDSRELALFLRYVYSCCVRASFSETSIMRIFDATVVGKVPVLDKIRDMAPATAAHVCAKDFLWIYGEHDFMYRACGRAAIEELRRRAHAGPSTPGEYRFRTVANAGHNMYLDNDGAFDREVIDFLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR00</accession>
    <name>A0A1E3NR00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019602.1">
        <property type="nucleotide sequence ID" value="XM_019160200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR00"/>
    <dbReference type="STRING" id="763406.A0A1E3NR00"/>
    <dbReference type="GeneID" id="30176887"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="113"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="444" mass="50133" checksum="6D36D8AC906BF20A" modified="2017-01-18" version="1">MATRNTVLEAELEPSVESSHRKPKSPSGVPLSQISPKIFPLPMSESFNHWWKQKTPLKVQDELLSTLPFYPEPSATHSSEVISFKSPNSLVFPDINEFHVKPRNETVGEENAKHLVIIHGYGAGLGFFEKNIESLAQKHLQWHIHAIDLPGYGCSTRVDFPHKIPYENHKTVEKLFTVPLRDWFVARGLSEKNTLAVAHSMGGYLSLLLQMNEVSGEGFINKDEYKMLKKRFGCGSAMEEAFQRQEKELSGTAANPRQFWDTLIQVSPGGIFHSRAKQIEENTPKWFTRLWNWNVSPFSLVRYSGPLGSYFVSGWSSRRIAVSSLFTEEDVKLLHRYSYAIFNAKGSGEYMLNYILAPGGIPRHPLVERLGSIGKYAGRTLWMYGDHDWMDYKGGVLACEELKAAGSPDAHLQIVAGAGHHVYLDAFRSFNQIVSSEMARMESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR31</accession>
    <name>A0A1E3NR31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_83465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019642.1">
        <property type="nucleotide sequence ID" value="XM_019164998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR31"/>
    <dbReference type="GeneID" id="30181685"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="281" mass="32412" checksum="208E3E23EE97DA76" modified="2017-01-18" version="1">MLLSIQPAPMTNDYSFSKQTQKQENRESPGEQWEQDVEQQRRQHDILRQQNQPSQVYPAYQDFQESREYRQPQEELTQQSRLPIEAYPRDYLGKPQGGYAVSTHQYLPPIQQSSRYLFSVPNHNGYVFQQIPADEPKYSSDYNYQVPSLQPSSQQQTLPLPTPSAPSIMSQYPPFPSLVNDAQRRDQLYANPQYYPAYNGAYLTHPSMSPIQSRSQSIQLNPTEALAPQQYYGQKIMVPLPEESYGFHSSNCSSSNSNCKKRTTNASTSYGQKRRTRGCWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR50</accession>
    <name>A0A1E3NR50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70188</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019675.1">
        <property type="nucleotide sequence ID" value="XM_019163582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR50"/>
    <dbReference type="STRING" id="763406.A0A1E3NR50"/>
    <dbReference type="GeneID" id="30180269"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="221"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="438" mass="50207" checksum="FD6D436C90729EBF" modified="2017-01-18" version="1">MSSGESVPILKYQVHDAMQNNDMILDPSCSGSGNPHSNQLQHVGSSVDYLGAQQADAHDLLQLPELPDFTQRPKTLLTNGLFPDSNEGMDQAYSSSTFDSNSPYRYQPETDRHSAFSEDLSPQRPFYSVQTSGTQSKNASVAFFSDALTDEHTYNELPLNEILDDFQYEDEDNYNTLKIDEESDVEGHSLNGDYPISRAETPDIHYQYLGKDINRSESPFLKKKEPEYVTEIPSFYSANLKYPYKSPLDYTDEEFDKMNHFEFLKDLKFEPPPLLPVYLNSNLLNDSNSKNYKTFPYQYQESTVPHVNEIYRYRINDLNLMDKYSAHKPSRPLLKRSGSSNSSCSSTSIHRSNKNGGKIRSKLLRENSNSSTKSNSRLKHADKMQLIERNLVPHHVMLNHLVTCNFNKEGYITSSCITRYKGKFITQIMYFSNELDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR63</accession>
    <name>A0A1E3NR63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAC3/GANP/THP3 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_22168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019674.1">
        <property type="nucleotide sequence ID" value="XM_019162238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR63"/>
    <dbReference type="STRING" id="763406.A0A1E3NR63"/>
    <dbReference type="GeneID" id="30178925"/>
    <dbReference type="OrthoDB" id="199574at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="1">
        <location>
            <begin position="10"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="170" mass="20482" checksum="8B4F01399BF91FA3" modified="2017-01-18" version="1" fragment="single">IVGKNQTLEKKYLRLTSQPKPETVRPLKVLHKTLQLLFDRYCEGAKYNYLCDQCKSMRQDLTVQNIKEDFSIMAYEFHSKLAIENGDWGEFNQCQSQLKLLYSITELHKPNYFEFLAYRVLYYILTYNYSEVFELELSLINERHPDLKNEYLDYALQIFKCVYDSNYYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRB8</accession>
    <name>A0A1E3NRB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14165</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019739.1">
        <property type="nucleotide sequence ID" value="XM_019160253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRB8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRB8"/>
    <dbReference type="GeneID" id="30176940"/>
    <dbReference type="OrthoDB" id="17415at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF5">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="522" mass="61180" checksum="1ACE3BB631A01B74" modified="2017-01-18" version="1">MKKDLSKTKDTFIHDYSVFPETLQYRLPTENEKGQPPQPHSNILKLIFEQGGVESMDLNMNQEKFSRYPMTTPAKLNKRATRPTSVKMLTSDFIDDSLYNVNYGYFAKEAVIFQPDRPFDYNHLAGQDEFMQKWLQAYDKYDQENTKTLQVIPRPKKSGKSEDKGGNQSKASGPAKTVETEQDKIVKPSLQLWHTPTELFQPFYGEALARYILVNYKLNKFPYNDLVIYEAGAGNGTLMLNILDYLEKNEPEVYARTKYRIIEISSKLFNKQNSRLEKHKDKVEVINKSILEWDTLVSEPCFFIALEVFDNLSHDVVRFDIDTKQPFQGYVVIDEHNDFQEYYSPELEPWTKGFLKLREESQYPIKSLSGHPINQFYPWMKLRNMLNPLRDNLTEPEYIPTRLYKLFKILKQYFPQHQILASDFSHFDKTIKGYNSPTVQTMYKDNMVNTDTYMVEQGYFDIMFPTDFRAINELYRQVTGNVSSTCSHAEFLTQWAEVEKTKTKSGENPMLELYSNADFIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRE1</accession>
    <name>A0A1E3NRE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_29088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019763.1">
        <property type="nucleotide sequence ID" value="XM_019162573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRE1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRE1"/>
    <dbReference type="GeneID" id="30179260"/>
    <dbReference type="OrthoDB" id="10258869at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="219" mass="25229" checksum="BD278D3E7F3D6E59" modified="2017-01-18" version="1">MARRPARCYRYCKNKPFPKSRYNRGVPESKIRIFDLGRKKASVDDFPLCVHLVSNEIEQLSSEALEAARICANKYITVQAGRESFHMRVRVHPFHVLRINKMLSCAGADRLQQGMRGAWGKPHGTAARVTIGQVLISIRTKDSNKAVVVEGLRRARYKFPGQQKIILSKKWGFTNLNRDEYVAKRQNNEVKEDGAYVKFISLKGNLEENFKEFPHYFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRJ2</accession>
    <name>A0A1E3NRJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_23867</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GCN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019813.1">
        <property type="nucleotide sequence ID" value="XM_019162327.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRJ2"/>
    <dbReference type="GeneID" id="30179014"/>
    <dbReference type="OrthoDB" id="5148094at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043008">
        <property type="term" value="F:ATP-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170011">
        <property type="term" value="F:stalled ribosome sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031571">
        <property type="term" value="P:mitotic G1 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904689">
        <property type="term" value="P:negative regulation of cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071264">
        <property type="term" value="P:positive regulation of translational initiation in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990611">
        <property type="term" value="P:regulation of cytoplasmic translational initiation in response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022716">
        <property type="entry name" value="Gcn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056810">
        <property type="entry name" value="GNC1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056809">
        <property type="entry name" value="HEAT_GCN1_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12074">
        <property type="entry name" value="Gcn1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24993">
        <property type="entry name" value="GNC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24916">
        <property type="entry name" value="HEAT_GCN1_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="4">
        <location>
            <begin position="1314"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="1489"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="1608"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="4">
        <location>
            <begin position="1636"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="1950"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="2353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2353" mass="259926" checksum="AF111ADC6DE35A76" modified="2017-01-18" version="1" fragment="single">ANEISPLLEQLRSSSTNTLNVSLFKLNSVIENEAEKLDNSQKDLILFVILSTYTRYPYDTKFNRLVLQSFKHFVANDTEKYSKNILGFIAKQSAKPVAARDLLTLISWNSAVTTMVSDKKLISSLLKVSVPVSVKLISLLLDYNQIDVVKVHQLRKVSSFKDDVVSSIRTVAKVDSGIISTILDSICDEKLPMSGVISMLGLLASATSKPNMGPELKELQSKEDSICNFFAKTCLSTKTYPATKSLEIFGTFVNNIITSDSLEKVILPGLEKAALRNSELTFAIFSPHIFRFLNYDSIDAISIVLKSKFFALPSSAVEQKVLVVNVINSVQYSFHSSTEKILDLTLPYVAKENNESYLPHLLDTMLSRFVHLVCEKITFDSKSKIVELLNSGLKEKKIPLKTAWCTSFGASILKQNITSENENGIFELFNSGITDSLLKVFNECVDSPLPSIHNKLIAGGYASIAVLFNLSEVFGNEELDNKLKESKIVERSLEETETKLSCFTNPKILTKLNTEESQEWFVRSLFSLSRHLVEPSALLGSAYIYTTLSRNVLHRVRLLSRTLLHKFYALKQNVVGSSLTSAVNELMKESTLNTELNYDFHYLLPAVSSTLYPLETNDPQLLESQLVDLLIPFEHAMLNSSSNGWVSLCQKCNVDPGRIVKEHAEEIVDRISAILADQSDEVFNNGTFEAARKTASIISFFSPESTVPLLSEQIQRDLSIVPKLEIDSTKLEIWKGRDGELVIDILASKAPVNIAKSKDSETLKWEQSVRKELEDKKKSVKKFTKEEQVKVKEQLELEKIIRQSVNSQYLKLRRGLELIVSLSKQAKSVDNASGVWFPIAISNILTLLNSKAAISLVGKLASDCFLILSEVVSNESLTGTSSMKFVGASTLRLHAIEDLAAEYKGKPLDEMLASQLFSLKLCSDKSLFNNLTLMYVLPLLVRIIENGKNYVKRIGDKSVKVNTEFSEEAPEEELLVMALEIISSNSDLFEDATIPRTVIIEHLIELLALPSKAKAAKECFVSLCQNISLNISDSDLNIILKSLISPIVFVRAAILEVLDQEFDLSKLDFNEELWISCFDNIETNKEVAEAIWSESEFSLKESCVEKLVPYLGAKDNGIRLSIAKSIASAITQLKSDRLFSSSLTGLIDLYRVMSKPPELETDEFGLVVKSNANQKDRWEQRSGVALTVTYLAPMFTKSEEVSKFFTFLIKERSLGDKEGLVRSELQEAGLKIIERNGKGNVEILITIFEECLAEKDEKSKVQDMVKESVIIMYGSLARHLDKDDSRIDAIVNRLLESLDTPSEDVQYAVSECISPLVPFIQTKMSSYIDNFFDKLFGAKSLAVRRGAAYGIAGLAKGIGIKALCEYDVIRNLTEASDDKKDNQKRESVLFAFECISQAFGPLFEPYVVEILPIVLKSFGDSSAEVREATDYASRVIMKNTTSYGIKKMIPLAISNLDDMAWRTKKGSVELLGSMAYLDPAQLSSSLPNIVPNIVSVLNDTHKEVRKAADAALKKFGEVIRNPEIQELVPILLKAIGDPTKYTDEALDSLISTQFVHYIDGPSLALIIHVIDRGMQDRSANTKKKACQIVGNMAILVDTNDLMPYLSRLVSELNDAVVDPVPQTRATAARALGSLVEKLGEDKFPGLMSSLLAALQDEEKPGNRLGSAQALAEIISGLGISKLEEMLPVIIAGTTSSKSFVREGFMPLLLFLPVSYGSQFAPYLSKTIPPILAGLADTLEEIRDVSLRAGRLIVNNYAKKAVDLLLPELEKGLSDSNARIRLSSVELTGELLFKISGISGKQELADDVTLSKNVAKAFNDVLGNERRNNILSSLFVCRSDTSGAVRATAANIWKALVANTPKTIKEIMPTLVVIIVRRLASPDDTQRIIVAATLGDMIRRVGGNALSQILPTLQETMVSSDSDAKQGICIALYEIINSTNSDNIIQHQDIFVSIVKEALVDPNPSVREAAAQTFDSLQESIGNSAVDEVIPQLLEMLNSSDGSEDALSALQEIMSTKSDVIFPILIPSLLTPPINGRAIGCHPLLQHILSLMKHEDIKKKNIIFDVLPAFFKETTLDYSIYTEDIVVHGTKDIYAFTLPRGPSCVLPIFLHGLMYGNPQQREISANGISIIIDHTPAAGLKPFVTLIVGPLIRVIGERFSSDQDMFSDGAGVNAAVAYAKLVGALSKILNKSELNNMLRNKIFSCDLEDDNSSRFAILTLNAFLKDSAESVLNSGLFPEICEFIISSSNSTKAYVSDNSTIAIGKVLLSLEKLEEVENSLVSDLVKQLCILMDKPNSSSSDTRRLSLVVARTISRYQYDTTRISAGGDKETMFSDQYEDETEIWAIGGVDLSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRJ3</accession>
    <name>A0A1E3NRJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PICMEDRAFT_14246</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019828.1">
        <property type="nucleotide sequence ID" value="XM_019160290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRJ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRJ3"/>
    <dbReference type="GeneID" id="30176977"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007006">
        <property type="term" value="P:mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902953">
        <property type="term" value="P:positive regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070863">
        <property type="term" value="P:positive regulation of protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00879">
        <property type="entry name" value="Sar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000161">
        <property type="entry name" value="Small COPII coat GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 21" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13 21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 19" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 21" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ48715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="189" mass="21643" checksum="3DF6601E34BEA97D" modified="2017-01-18" version="1">MWIFDWFQDALAYLGLWNKHAKLLFLGLDNAGKTTLLHMLKNDRMATLQPTLHPTSEELSIGNVRFTTFDLGGHQQARRLWKDYFPEVNGIVYLVDVADPERFEESRLELDALLHMPELATVPFLILGNKIDSPRAVSENEVRNYLGLLQTTGKTATVPNGVRPLELFMCSIVMRQGYAEGFKWLSQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRP2</accession>
    <name>A0A1E3NRP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019865.1">
        <property type="nucleotide sequence ID" value="XM_019163620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRP2"/>
    <dbReference type="GeneID" id="30180307"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="424" mass="50077" checksum="5029416C17698627" modified="2017-01-18" version="1">MDNKQNQFTINNNYRSKSVTRISELPDYPVDETIVGLLLGVSRNRPPSKAMNYPYTLHMTDFSDSRFKFPQYFDGRDFKKKELQHKTFDVVTFPAVFDTISKDVRDIVDLHGKVRYYKFQNGRPYYEDEWNQEDLDVLYLKLGIENKLAGKELGIDYWLLDKFQIWIKVRIRGKRYKEQMELVCMNNFYVIPELILQRELRSYGQQWVWEEFVRVYNHKDLLDEDFSDSDVETPSTAGETPRGEKRRKLALAEERPQQREKDTARRFDDIQYTLVSELADDNVWAERASVQYAPQYILVRRVRIWKKLDSYVYMHRRALGVKRAKFVVADERGHELVLTIRENCLTGFLGLSIGDFKFFEDLNEAIKTRVEQWEGSSGSADAYHGMVVSRARIAPGVHKWCWEYTTAEPVPAHQHHTPSRPELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRP9</accession>
    <name>A0A1E3NRP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-serine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-serine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_70385</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of D-serine to pyruvate and ammonia. May play a role in D-serine detoxification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13977"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="EC" id="4.3.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:13978"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DSD1 family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019888.1">
        <property type="nucleotide sequence ID" value="XM_019163626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRP9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRP9"/>
    <dbReference type="GeneID" id="30180313"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06817">
        <property type="entry name" value="PLPDE_III_DSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000016">
        <property type="entry name" value="D-serine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0216">Detoxification</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="14">
        <location>
            <begin position="298"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="417" mass="45632" checksum="1BF3E7B68A727DC0" modified="2017-01-18" version="1">MSFPYEFTPLADKQTLVKAYQGKKKSELPTPSLIVDRTIFRDNCNKMLQNAAKLGADFRAHVKTHKTLEGTALQLGSGDASTDKIVVSTLMEAWGLLPLIKQGLINDVLFSLPVVESRLPELVALSQHVPHLRLMLDNYAQVETLAKFSKAHGYSKKWSIFFKVNMGTNRAGLGVDDAELDKTLTAVLSSPLSDYVSLYGFYCHAGHAYSSCNCDQAESFLVDEITHGNLACKKALAIDPSLKLQLSVGSTPTAHASEVLEVSKLGPLSGQLELHAGNYPFCDLQQMATHCVSLSSVSCRVLADVLSSYPQRGKIEPGEQLINAGVIALAREFGPVPGHGRVYSPEGYENWTVGRLSQEHGILVPLEESKDTKLIPLGTRVEVVPQHSCITAASYPWFFVFDGDDVVADIWVPFRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRU9</accession>
    <name>A0A1E3NRU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_87182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019914.1">
        <property type="nucleotide sequence ID" value="XM_019165024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRU9"/>
    <dbReference type="GeneID" id="30181711"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="89" mass="10244" checksum="A20F039665E5F49B" modified="2017-01-18" version="1">MPALSLTIQKCRSFSNGWLQLVNPTVVNVSVQQPALSLVQKIWARRQDRSSRKLFWPASYRCCLETLPAARCTIRARCSTTQKRYTNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRW0</accession>
    <name>A0A1E3NRW0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019924.1">
        <property type="nucleotide sequence ID" value="XM_019163632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRW0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRW0"/>
    <dbReference type="GeneID" id="30180319"/>
    <dbReference type="OrthoDB" id="3998223at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="914"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1131" mass="126494" checksum="73E55795BE3E4BA1" modified="2017-01-18" version="1">MAQEKLLDLSVQGGINTHSFDSPDLSKEVVTLDMREKTSGQDMFSQYLNVSDHMNSLESSLTWVDSGMNTVEHLPRFERQSDSALMISNKIKEKGKFILPLKQKKVSDKLKLLKESRPEADENLVNMNHFPAKPLSTLNLRPNRNNISSQLLSSSNLASSKRSDSEVDQTSHSKIHIFSPQPKIEQEFSNSAELENGMKITSRRDSLIEHSSDIEDGNEVEHSSSPTIPVDSPSMHVFSASNDESEYSNINNSSDKFQHQFMAQPGQIFQEPPTFANAEVNKSVRNPASTNKTNISFSIATNPIESQKNTFTPSTPEDKVVILKLLRDGIRDYSSLLTDFSENMVVENRSDYLVSYVKRFDYELKPKLINDYLGEIRKDKISEDVKQILNNDTFCEEVKQLLIESDKRRKCYNKRKSARSSESKKSSPVFTSTQSTPLLGRSSTFANVTTDTSAPSSAKSTTVKLLPKVEASKPGKSKAIVINLIRIGSFINQRQLKKSGHGNENLVAIADLRNIAKKNDDERYIHKLRATNTSLKMCVEDIYGPNANFTHSLAYSRIPLSLDPLTSINLENDQMISYVEILLDVSKMLIPGNRSINSSLNYASILQLKSVTRIYSEDRLVYRRCDPITGFFTKDNVNVAKIILPLQAKLWANLINDYHNGIINDSKVSALKITHTLFPNDDILRFKSGGSAIYSFVWEFVINVDGHYLPHCVNIEKLMPSTNNFAKVNVQSVANSELKFGVPQPHAQFQQNSTSLNYVAPPNLGAINLPSHQQSSQKHIAPTKTQSLRRAVTHGTINIAANVKTNNNLDMPIDNFNVNLNNQPPAYSRSNSALNNPSVSSMVTSTPLLTQRKREDLGRGHKRTRSRSMNEIDLMSLPLSFDGFMNMPFLDSFSPTESSSSPLVKQNIPYLKSQSVQHLHQQQLQPQPHLQQPQQLQHSSSLHVTQPQPQQQQWQALKHSNGIYSINPNMGGHGYGRPVYQPIMEYKHFNNSDSQLELNNFHSSFEEATANSLVINTAPTHISPKQMAFSIESLDDSIKTSDSNIILGNDNISNNNNNNNSTSSTAIKSTITNNTNWSKLPTIPDVSLYASSSSAMPTSFDFDENSKAIAELSVLSEIPELTTPQPNQKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS05</accession>
    <name>A0A1E3NS05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amine oxidase</fullName>
            <ecNumber evidence="11">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_70423</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="11">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="10">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10 11">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019937.1">
        <property type="nucleotide sequence ID" value="XM_019163637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS05"/>
    <dbReference type="STRING" id="763406.A0A1E3NS05"/>
    <dbReference type="GeneID" id="30180324"/>
    <dbReference type="OrthoDB" id="5379943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049947">
        <property type="entry name" value="Cu_Am_Ox_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF33">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01165">
        <property type="entry name" value="COPPER_AMINE_OXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="12">
        <location>
            <begin position="234"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="9">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="10">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="667" mass="75885" checksum="04CCFD88180D2174" modified="2017-01-18" version="1">MAPHPYDQISDEELRLIVKLVKDAYPSEKPSFIQLDRLDPPKNVMLQYLSAEKQGVSPPMIPRRAYAYFYINQVFHKALVNLTYKHIICDVKLPEGVVGPLVADDVIVVEDLATNHPICRAEIEKLCLPDNIHVVCDPWIYGTDDPNEKRLLIQCYMYLANKNHPESNHYSLPLKYSPVFDGLTKEFVRMDYLPSGADEAVINETTAWDEYPLVEYHPDLNGCTKLRDVKPLIVSQPEGVSFTVDNHKIEWQDWTFYVVPNSREGAVIYDIHFKGRSVIYRLSLSEMTVPYGDPRGPFHRKQAFDLGDCGFGVNGNTLGLGCECLGVIKYFDNRRVSTAGDVEIIPNTVCMHEQDAGILYKHVNYRTGHSVVARRREFVVQIIATVANYEYAVQYIFDQVGEIKISVRATGILSTMPIDRDVDVDYGTLVGPRVMAAFHQHLLSFRIDPAIDGHKNTVVYDDILRMEENTPLNPYNVGFYSKRSYVEKAGHIDQSPFTNRAYKIINENVINPTSKKPVGYKIVMPARQMIMASPESYNTKRAKFATEQIWVTKYEEDRLYAAGEFTNQSQKDTGIAEWANGVDNVRDTDNIVWCTLGFTHVPKPEDFPVMPVEMHEIGITPFSFFEKNPALDLPQATNKFNKSVLFEENERSKRSSTSSTCCKKASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS42</accession>
    <name>A0A1E3NS42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_57026</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019977.1">
        <property type="nucleotide sequence ID" value="XM_019163209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS42"/>
    <dbReference type="STRING" id="763406.A0A1E3NS42"/>
    <dbReference type="GeneID" id="30179896"/>
    <dbReference type="OrthoDB" id="10256524at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00306">
        <property type="entry name" value="Peptidases_S8_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010435">
        <property type="entry name" value="C5a/SBT2-like_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06280">
        <property type="entry name" value="fn3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133497" description="Peptidase S8/S53 domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="4">
        <location>
            <begin position="117"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C5a peptidase/Subtilisin-like protease SBT2-like Fn3-like" evidence="5">
        <location>
            <begin position="473"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="88308" checksum="DF68E695D05E993D" modified="2017-01-18" version="1">MKLATSLLLALTGSCLGSSFNTSSIIDYNVTRSSYHIAVGNDTDYSYWSLMATNASLSYADGYNVTVAIIGDGVNSKYLLSNDSVHSVLDVYSNSTYLFSENELTEDTLAAKVLLANAPATKIKSYKIFKNSDSTTSITSKLVSAVLTAVNDKSVDFIYINTWQAYGGWSESYLSELMNEAGRQKPIVLPMGQSNGIFQFSDGAAAENVIAVGGAGYEGVPGFATDIDGEIYGVYAEKAMNLWANNTLVLGQLDSNCRLKSNVSDHESNLLFIDVGNCSSDQIVETVIESNFTQLLTTGEEYQLYNFSAKSGYTSAMIGRENGMKILKRLENGDTIVAHKRFDENMIVVDYEFDEYSINFNSSMGPTLDLYLKPNIIAQSAFFFDDLNVYASGTSLSAAYVAGVVAAYASGMRDKSWYNGIVDRVTTSGDQVNFRNLTHTSSEIENPIVQGGGIINAEDFVLKNYDISPGFFALKEAKTTSEITFSITNNNDKAAKRFHISGLDAITIYSQDLDIDANNNNMYPFPIYGNVTAATVVIEKNTILVPGGKSVNVTVLITPPNYCSEADRIPVYGGYIQVKDLSTNIVSSIPYFGSTVEFHSLETQTDEEFYWKEFHYEPDGRYCDMWVNGSVVNDTNHFMQIYFNDRIGSPLIDILVVEKDWTETDFVYPPVPGQNKFIERIQGTRYPGGYPWHYAPRSNTTLDDQWDDWHDGLLANGSVLPAGEYKILLRILKHNVQDPSDWDEWYNHVSPWFVMDYANVTEVDLPLTTISISQTTLKTVSTSAAAASTSSMDMAGMHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS85</accession>
    <name>A0A1E3NS85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-coenzyme A transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_30083</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019556.1">
        <property type="nucleotide sequence ID" value="XM_019162626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS85"/>
    <dbReference type="STRING" id="763406.A0A1E3NS85"/>
    <dbReference type="GeneID" id="30179313"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000289">
        <property type="entry name" value="Acetyl-coenzyme A transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="63053" checksum="396C208369B564C0" modified="2017-01-18" version="1">MEIDEDHIQSISSQLDDSDAPRGKPRPNDKRDISLPKQDYPRFFLLVMLYFIQGIPIGLAFGSVPFLLKSNKLTYSQVGLFSLATYPYSLKILWSPIVDSCYSATVGKRRSWIIPIQCVSGVSLIFLGSKIDTWISDNDLILQNLPLLSFSFFFLILLCATQDIAVDGWALTILSKNALSYASTAQTIGLNTGYFVSFTIFLAFNSEDFANKYFRSVPQDYGFVSLGQYMMFSGFLYLFITLLIVMVIPENPPVKKYRDTTEYNLEYDTADTDDSIKTVYSKMLKVLKLKNVQTFIVLHLVSKIAFQANEAATNLKLLDKGFSREDLAITVLIDFPFEIVFGYYAARWSNTNKPLKPWMYGYIGRLVAAIFGQILVWGFPKSGKISTSYFVFVILQHLLSSFMSTVQFVSICAFHTKIADPLIGGTYMTTLNTLSNFGGTWPKIIVLYLIDKLSKSICVDPNEETSSPIKGNQNPFFNEKFYSCYQNDMKSLCTENGGSCIPVKDGYYYTNLLCIVLGLVLYFGWIRKTAKSLERLPIASWRVKEKGALNGIRSHLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS97</accession>
    <name>A0A1E3NS97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14524</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020140.1">
        <property type="nucleotide sequence ID" value="XM_019160413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS97"/>
    <dbReference type="STRING" id="763406.A0A1E3NS97"/>
    <dbReference type="GeneID" id="30177100"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000001">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="363" mass="40477" checksum="EEE44DFC0AFBAA5F" modified="2017-01-18" version="1">MSTAAESQENRSRVYFDVSIGNEPVGRIAFELFNDITPKTAENFKELCTGEKGFGYKGTTFHRVIKDFMIQGGDFENGDGTGGKSIYGEKFDDENFQLKHDQPFLLSMANAGPNTNGSQFFITTVKTPHLDGKHVVFGKVLAGKSIVRQIERSETAESDRPLKECKVIDCGELKEGDPLTLPDGTGDKYEDVLADEPTVNIDKPETVFQAVEAIKNIGTDCFKKGDYKTALRKYIKAQSYMAEYQPYDLTDEDVATYWKLQISCNLNAALMSLKLKDSQDAIKYSTEALSCEQLDEKSKAKAFFRRGNGYILGHDEEDAIADYEEAHKLQPNDAGITKALNDAKLSLKTRKAKEKAAYSKFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSI2</accession>
    <name>A0A1E3NSI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Polyprenal reductase</fullName>
            <ecNumber evidence="5">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_95512</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.94" evidence="5"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020230.1">
        <property type="nucleotide sequence ID" value="XM_019165100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSI2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSI2"/>
    <dbReference type="GeneID" id="30181787"/>
    <dbReference type="OrthoDB" id="541710at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="158"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="271" mass="31397" checksum="F039FC3B66AF8C90" modified="2017-01-18" version="1">MFQIEAVAFWSINVISVGLALMTLSVLLVSFFPAINSFLTYGKTLKIVNHKKTKRNNLANLSIVHELYVPKKWFTHFYVIHFVLSLVNTYVIVFPNSQLTDLQVIALLNLAQSIRRVYECQRVSRFSSSAKIHIFHYLVGLIFYASVDVLPFMMHLLHEENTISFGYILIPILVFFLASCDQLFTHIILSKQKKYTLPDVGLFNYVVCPHYMDECLIYFSFLLVRPCIPLLMVMTWVVINLSASANQSYIFYKNNETSNISSRYRIFPGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSL4</accession>
    <name>A0A1E3NSL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020204.1">
        <property type="nucleotide sequence ID" value="XM_019160443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSL4"/>
    <dbReference type="GeneID" id="30177130"/>
    <dbReference type="OrthoDB" id="3993984at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="333" mass="37730" checksum="498D7F2494F39C47" modified="2017-01-18" version="1">MGNSASKTTRILDKGVKLNHQPSTLGQGTINNLKKQVTADHLKNKIDTRNSEASEKKESKHQVFTQRPPSELEQMGDSDSAANGTHYEGNRLFEKAVSAGLVKVQDSTHKQSYNPNHESLQVLKNRVNVEKQYEGLFIPKDADKPNVPERFLNEEQKKSSLDPEAMLKKTKKTGINSFGLFDSGVLSDLIVDYKVFGEAKYIEKAKEIDANDENIVKLKKFIDDGIINLPTHKVTLKDFADPASKQMKQKLVTVKDDWVKTIKEDIEKEKINKLDSKSTTKTDLEVFEQFKMLENLVSKSQISTKKVDGPLEESELVMNRRKKQVVKEVKKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSM8</accession>
    <name>A0A1E3NSM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14134</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019696.1">
        <property type="nucleotide sequence ID" value="XM_019160237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSM8"/>
    <dbReference type="GeneID" id="30176924"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070158">
        <property type="term" value="P:mitochondrial seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="193"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="318"/>
            <end position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="334"/>
            <end position="337"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="407"/>
            <end position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="492" mass="56018" checksum="61A5A2FE88741744" modified="2017-01-18" version="1">MWRRLGHRPYGTAKVASNVDMKQYAETAASLRTTNSLKKAQLDLKRAYQEYDTFKDVCAKRNMTFPLSPTFRDDYQELQKISETVLNFKHESRQLQGTLKQLKIAQKKDKVDNIVEQETILDKLTRYKPILNEKTKLQTTLEDKLFVQVSSLPNYIHDSVKDKQVLLEYLNPTQELKDSTSDDFKVKSDSRFDHKEIMEHLGLVNFSQATKVSGRGWYYLIGDGALLEQALIQYALKKARQAGFLMVLPPSIVRTEVTNACGFMPRDTNNERQVYELQNEDLCLTGTAEIPLAALYANHEFKGADVPLDLVGLSRSYRAEAGAAGRDTRGLYRVHEFTKVELFSWTFPDEKQSMDKFDRIVKFQKDFIKSLGLTARVLIMPHDDLGAPAYKKIDIEVLMPGRGTWGEVSSTSNCLEFQSRRLNTKVRDPKDSKLKFAHTLNGTACAVPRVVLAIIENFYDPATDSIQIPEVLRPYMDDKTEIGGKTATSIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSW3</accession>
    <name>A0A1E3NSW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70750</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020304.1">
        <property type="nucleotide sequence ID" value="XM_019163727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSW3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSW3"/>
    <dbReference type="GeneID" id="30180414"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF18">
        <property type="entry name" value="ACID PHOSPHATASE PHO11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00778">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="398"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="448" mass="52012" checksum="703FC942868020AD" modified="2017-01-18" version="1">MHFGNIIPASILINQYTEHFVSPIHADQNNLLKHLGGMGPYVKGTGFGLCPELEWNAKPEKVFIFARHGERYPTEPVAKRLKAVFDKLKNADVKKQEGPLSFIKDWEFFIPGPDWCGMETLYGDFNGFQTMFDFGKSIQLKYGYLYKDCDEKLPIFTAGKRRCVDSAEEFGKGFFNDNDEKYNAQAKVIVLDETKRMGADTLTTSVSCKKYIRHGEYYKHPFIGKYMISEAKRLNTLSPGFNITAKDVYALAVFCAYELDAVGESRVCDALSLNTFVELEYFRDSEMWYEKAAHPYSFALGSVYVDAMIKLFEEQETKENLFFSFTHDTELLHFMNALGIFEYQKEDLSTRNIDFLRFFKTSELVPMGARIVIERYTINKEHYIRVLINDAVIPIPECQNGPFFTCKLTKLNHIVSEKMKGNNFVDKCRITEERPQELTFYWDWRERC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT40</accession>
    <name>A0A1E3NT40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_99118</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020418.1">
        <property type="nucleotide sequence ID" value="XM_019165143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT40"/>
    <dbReference type="STRING" id="763406.A0A1E3NT40"/>
    <dbReference type="GeneID" id="30181830"/>
    <dbReference type="OrthoDB" id="419770at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016480397" description="Post-GPI attachment to proteins factor 3" evidence="7">
        <location>
            <begin position="24"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="364" mass="43060" checksum="CA2AA259F6104EE1" modified="2017-01-18" version="1">MRTSTQFAVALASVFSVLQLTLASQGDQLPEFKRCVKYCDILTCDNVDHYPDVIPSTYMNLLKDENLVNLFEKTPISFHLSLLGWDCSSNCDYQCQRLVTDDRIAEGLKVYQFHGKWPFVRVLGVQELFSTLFSIGNWIPNFLGFKLLWKQYNAELRKTNMDMANLYWTYMHIAVVSMCAWFFSTIFHLKDTWDRERLDYFFAGMTVLTGFYAVSVRFFKLYLVENNMKRKIFGTICILLYLGHVTRLLFDWSYTYNMQANVVIGLVQNFLWVYLSIYQFQKISSSKLSFIENVTRQEYNWTLTPTLLVLSVFFGMSFELFDFPPVLQLLDAHAMWHFVTIWPTLFWYPYLVKDAQELMDWKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT73</accession>
    <name>A0A1E3NT73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_29331</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020422.1">
        <property type="nucleotide sequence ID" value="XM_019162587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT73"/>
    <dbReference type="STRING" id="763406.A0A1E3NT73"/>
    <dbReference type="GeneID" id="30179274"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000224">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF34">
        <property type="entry name" value="PROTEIN HOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="446"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="71"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="570" mass="63174" checksum="1FF0B724CBBD5955" modified="2017-01-18" version="1">MNKASTFIDNDKYINKYHPEYVPGTYNILLEKDEKGVALSSGLKTTKNGIILNPQPTDSPNDPLNWSPWVKAYQFALLIFITAFTAATSNDAAAIQDTMNERYGISFGAMNTGAGVLFASIGLCTLILGPASSLYGRKITYMICILLGLCGAAWFGAAKSTSDTIWSQLFVGASEGCAEANVQLSISDMFYTHQLGWALTAYIIGTSVGTYLGPLIAGFIVQYATFRWVGWVAVIISGGLLAVIVFTQYETYFNRTRYVPSSITATELNEIPHNQIDSQNLSQSEPSEGFYILNNGADEKRKSFWQRIALITPSTNLKGFGVKQYFERLVLMLRVFWFPPVVLSGVLWGLQDAFLTFYLTTEDDQYYDPPFNLSDTGVALMNIPCLIGAVIGCFYAGILSDYFVAWMARRRNGIMEAEDYLYFLIGVFIACPIGLMVFAAGTDQLWSWRVTYCVGLAFLGFSFGCSGDIAMSYLMCAYPEMVIEGMIGVSMINNAIGCIFTFVCSLWLGAMGNTHTYAILTGIQVAACLCAIPFLIWGKQMRLWTRKYYLNFVEKRDGVKKEDIVNDTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTD5</accession>
    <name>A0A1E3NTD5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histidine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_14781</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the aminoacylation of histidyl-tRNA in both the cytoplasm and the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020427.1">
        <property type="nucleotide sequence ID" value="XM_019160527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTD5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTD5"/>
    <dbReference type="GeneID" id="30177214"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000015">
        <property type="entry name" value="Histidyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="56"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="114"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="315"/>
            <end position="316"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ49314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="540" mass="59797" checksum="22342FC74C091B30" modified="2017-01-18" version="1">MSTEAVKDTVAAATVAAAEPAVKNEQKPKKKQQEQQAFTLKTPKGTKDWADKDMIVRSAVFDHLTSIFKSHGGVTIDTPVFELREILSGKYGEDSKLIYNLEDQGGELTSLRYDLTVPFARFVAMNGISNIKRYHIAKVYRRDQPAMTKGRMREFYQCDFDVAGQYDSMVPDAEMMKILCSGLVGLGIEDFKVKLNHRKILDGIFEVCGVKAEDVRKISSAVDKLDKSPWEAVKKEMVVDKHQPEEVADKIGTYVKINGTIQDVLATLKADETLMSNASAKTGIEEIATMAEYLEAFKVDKFISFDMSLARGLDYYTGVIYEAVTEQSAPPEGANEKKEKAKSKAKKSKDNDDDASEYVGVGSIAAGGRYDNLVGMFLGSNSKGKKAPSIPCVGISFGVERIFSLIKQRQALVDKIRPTSTQVYIMAFGGGEGWDGFLSERMHIAGMLWDAGIESEYLYKKKANPRKQFDAAEKSGCPIAVILGKEEYPKGEVRIKVLGQNEENEGDVVKIEDMVDTISEKLSQLKINGLSDVERLLQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTF3</accession>
    <name>A0A1E3NTF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Presequence protease, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Pitrilysin metalloproteinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_14885</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Degrades mitochondrial transit peptides after their cleavage in the intermembrane space or in the matrix, and presequence peptides; clearance of these peptides is required to keep the presequence processing machinery running. Preferentially cleaves the N-terminal side of paired basic amino acid residues. Also degrades other unstructured peptides. May function as an ATP-dependent peptidase as opposed to a metalloendopeptidase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer and homodimer; homodimerization is induced by binding of the substrate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M16 family. PreP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020538.1">
        <property type="nucleotide sequence ID" value="XM_019160581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTF3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTF3"/>
    <dbReference type="GeneID" id="30177268"/>
    <dbReference type="OrthoDB" id="10250783at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000013">
        <property type="entry name" value="Mitochondrial presequence protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000009">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000011">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013578">
        <property type="entry name" value="Peptidase_M16C_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055130">
        <property type="entry name" value="PreP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF13">
        <property type="entry name" value="PRESEQUENCE PROTEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08367">
        <property type="entry name" value="M16C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22516">
        <property type="entry name" value="PreP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01264">
        <property type="entry name" value="M16C_associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16C associated" evidence="16">
        <location>
            <begin position="488"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ49425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1006" mass="113344" checksum="ECF7426AF3FFE452" modified="2017-01-18" version="1">MLRFLSKKASLSFSRSFTTKADSRILKKYSVGSNIHGYTVQRAEHIPEFNLVAVALEHEKTGSKHLHIDRPDDNNVFGIIFKTNTPNDTGLPHILEHTTLCGSEKYPVRDPFFKMLNRSLSNFMNAMTGHDYTFYPFATTNVKDFNNLMDIYLDATLHPLLTSEDFYQEGWRLENEVTKDKFSPLVFKGVVYNEMKGQVSDSSYYFWIKFQESIYPSLHNSGGDPSQITKLDHGDLIDFHSACYHPSNSRTFTYGNIPLNDHLKKLDDAFVPFGKRVSHNVLRQPVDYTENVDLVVKGPLDPMLPPNAQYKTSLTWKAGSPTEIYETFLLKMVSSLLTDGHSSPLYQGLIETGLGTDFSVNSGADSITASNFFTVGLNGLIKENSDKLEEKVIAILQKVKEEGFSDIKIKALIHQLELSRKVENASFGLNVLSSLVSNWVSNVDPINSLKWDDIVLKFKDDYSKRGDFIFKDLIEKHFLTKPYFKYTMKPDENFSKLVAQEEKERLKSKVEKLTEEDREVVYNRGIKLLEKQEEKEDLSCLPTVNVSDISREVPFLRINNKYEQGIPIQSRLSPKTNGLSYFRGLKCIKASELNQDLISYLPLFSSCLTNLGTNSKTMADLEDEIKLTTGGLSSSFFTHASPYDTNEVYLKFSLSSVCLDSEFGKMLSLWQQILLDTNFKNISKLSTLIKSSTADSLSDIVSGGHSYARSAASSKVSPVGNIQELLGGIHSIKFMNELAVFETEGSLAENVVPKLEKIKEILLSGSGLQYSVTTSKDVIRAQESQVLKFNDQLNFMGSFEKKPYEFEVPSGVILKNQFIKIPSHVSFSSTALCAPSYSSKESASLQILSQLLTFRYLHGEIREKGGAYGAGASLDSLNGLFSFYSYRDPKPLNTMNIFDQAVSATSSNIRKNQITEEDLEQAKLTIFQRIEAPKSVRDDGMSLFNYDIDDDLKQERRIDLLDCSLDDVLEAGDRYFSESSSKSRVVIGHDTSEIEKSSDWVVEELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTI7</accession>
    <name>A0A1E3NTI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Protoheme IX farnesyltransferase, mitochondrial</fullName>
            <ecNumber evidence="11">2.5.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_66604</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Converts protoheme IX and farnesyl diphosphate to heme O.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ubiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020487.1">
        <property type="nucleotide sequence ID" value="XM_019163492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTI7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTI7"/>
    <dbReference type="GeneID" id="30180179"/>
    <dbReference type="OrthoDB" id="5211at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000004">
        <property type="entry name" value="Protoheme IX farnesyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016315">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001773">
        <property type="entry name" value="COX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="482" mass="53588" checksum="887AF23D552F08B6" modified="2017-01-18" version="1">MITKPQFSKIPLFSSVRLYASTSSASSASAIYTPLSEFTNIYYSNNTVNQSTLNFIGRSSSSSLRFTPNYRILAPTLLKHSSTRSIYSTPLSTSETNRVIEKKSPLDAVSGICHPPAEPSSNTTRDGASCTAKSHIPFEIKVKSQKKDDRSILEQYMQGEPKTLKQLMNPYMTLTKPHLTFLIMLSSICSYTLAPNAVDVSKFLYLTVGTLMTSGAANAINMGREPNFDSQMVRTSTRPVVTGLVKPNEAFGFAALTGTIGCAVLYYGVNPTVASLAAANIVLYSWFYTSLKRKSILNTWVGAIVGAIPPLMGWAACSSLTDPGAWCLALLLYAWQFPHFNSLSHNIKAEYKRAGYVMTAFVNPNLNARVALRYSIAMFPICFAMVYYNVCDIFFAVDSSILNAWMTYNAFKFWLQQRHNYSKNVLEHGGPTKGEIDLANVWAKKTFWSSIWQLPVVLALAMIHKEGQWDRFFGTSEERPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTI9</accession>
    <name>A0A1E3NTI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020578.1">
        <property type="nucleotide sequence ID" value="XM_019163799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTI9"/>
    <dbReference type="GeneID" id="30180486"/>
    <dbReference type="OrthoDB" id="3988723at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="131" mass="14677" checksum="27C8DCCDA05C7368" modified="2017-01-18" version="1">MSAHKYLTPEPVRYDPAIDPNNMSQYAPKDYRAVPVYEKVCNTHGATGDRKQEAMLPKYFEAIGDIESLGPAPPYKQECDSEGTQTAGRRVKNTRLINALHFSVYILVVVLIQAGFIWKAVESRSTTTTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTJ7</accession>
    <name>A0A1E3NTJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_101676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020554.1">
        <property type="nucleotide sequence ID" value="XM_019159643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTJ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTJ7"/>
    <dbReference type="GeneID" id="30176330"/>
    <dbReference type="OrthoDB" id="3995133at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="360" mass="39513" checksum="FFC17F6DE90200B1" modified="2017-01-18" version="1">MASSKRTVTTTTHSSLSSLSTHSSASASATFTPVISHEYNPFVYHYDDKNGTVFIAVGTIIVTLFVLLLSARFWYWLKNRKAAQDATRFDDYYGGGNVSYYDDDKSDLSFLTYDPTSFMEKKNSYRNENATPNSDSSGFTHNSTASSSSFGSMNDNQSRVDLSNLTSQPGHNLRSALAQSYIPPKNRHSFISPINQLIQEQNPSMQSVITDSGGSKRLSSNQLLDAFNIPSPVDPSVNISSPPTSATSKDSRRRKTQSISLLINSSSPNMNSKSNLPVSNNHSTNTLGGAHSRSTSLDLRELEKMIHRSLAENKSKDSVESASPASVSSPMPSSNREENKKRTRPPSLVLDMLVQNEMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTK2</accession>
    <name>A0A1E3NTK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribophorin II C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_57511</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020598.1">
        <property type="nucleotide sequence ID" value="XM_019163243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTK2"/>
    <dbReference type="GeneID" id="30179930"/>
    <dbReference type="OrthoDB" id="432292at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="8">
        <location>
            <begin position="153"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="258" mass="29231" checksum="D3FCD73A4E49A321" modified="2017-01-18" version="1">MPCQALKIGKGLVRIDDQNFHLLDENIPLNITDTKLSIGFTFSDETAGADTFLTPQQAVITLSTDNVESEMYLYPSLVEGKVYEVNVPIARISKLLLSQEKIHITVISGDPIDSSLNAILKAGTLNPSSKLRAEKSVDFPPRFGVKDEIHHIFRQDPKNLPGFISTNFVFLLLLSLFVLFLSWNYFEAANIDNSSKFNPVVYIFLGSVLAFEYYFFDYYCGTSIFKSLKRFTITGIFALYFGTKALKDMYRLRSQNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTL6</accession>
    <name>A0A1E3NTL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein AF-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14917</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020584.1">
        <property type="nucleotide sequence ID" value="XM_019160595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTL6"/>
    <dbReference type="STRING" id="763406.A0A1E3NTL6"/>
    <dbReference type="GeneID" id="30177282"/>
    <dbReference type="OrthoDB" id="16041at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16908">
        <property type="entry name" value="YEATS_Yaf9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="6">
        <location>
            <begin position="10"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="209" mass="23913" checksum="78A7B2377FBE415E" modified="2017-01-18" version="1">MAPPASSHKRIKQVSISKPIMYGNVATKFSPINPMPPAAPKDHTHSWTVFVKDPTGNDLSTYIKKVIFKLHDTYPNSSRTVEQPPFEVTETGWGEFEIIIKIFFHTEGGEKNITLIHHLKMHPYNGLVSMDGKVESVRYDEIVFNEPTEKMFALLTKQPGTLLDDKLKTREREELDRIAKAIDDIAEKTKTSTEEFKKLDKERKSLVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTN4</accession>
    <name>A0A1E3NTN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein bL33m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14858</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020503.1">
        <property type="nucleotide sequence ID" value="XM_019160568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTN4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTN4"/>
    <dbReference type="GeneID" id="30177255"/>
    <dbReference type="OrthoDB" id="275534at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="entry name" value="Ribosomal protein L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052008">
        <property type="entry name" value="Mitoribosomal_protein_bL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="Ribosomal_bL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L33, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL33M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="75" mass="8685" checksum="2A7B26C9D5D12712" modified="2017-01-18" version="1">MAKAKARTTIVQLVSTAKTGYRRTLLVPRTAREITQVRYDPVVQRHVLFVESKKRKGEQPKALDFSRGAFLWKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTR4</accession>
    <name>A0A1E3NTR4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_9934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020585.1">
        <property type="nucleotide sequence ID" value="XM_019165150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTR4"/>
    <dbReference type="GeneID" id="30181837"/>
    <dbReference type="OrthoDB" id="3997115at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="251" mass="28802" checksum="764399696D44AA58" modified="2017-01-18" version="1">MIRDNSYAFIYEDDDDCIHAQRGVKREHTSELITPLSTYNYTHKLYNHNDQLDNVGSKRRRINNTADSGLCSSLKKSKTGRRIPEIVIKQAHLEKSQLKDIFETFVGLEYIPPKCGNKTMLDKESADFFENRISVLLFLFDCSNFSYLKFFELFPQKNIKVIGITPEFGLYNDQSYPIVLDKNGDIAKQLSIRNPLGGGIYPIPSIILFDSSQTEIFRIKLGYDYNVYYDSSVENNLQSVLIEGVNYAVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTR7</accession>
    <name>A0A1E3NTR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70543</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Involved in nucleotide excision repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RAD23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020066.1">
        <property type="nucleotide sequence ID" value="XM_019163669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTR7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTR7"/>
    <dbReference type="GeneID" id="30180356"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120125">
        <property type="term" value="C:PNGase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14280">
        <property type="entry name" value="UBA1_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.540">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004806">
        <property type="entry name" value="Rad23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036353">
        <property type="entry name" value="XPC-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00601">
        <property type="entry name" value="rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01839">
        <property type="entry name" value="RAD23PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101238">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="133"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="330"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="80"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="179"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="374" mass="40146" checksum="819DDD8295CADCD8" modified="2017-01-18" version="1">MQVIFKDFKKEKISIEIEPTDSVLSGKEKLASIKNCDADQIKFVYSGKVLQNDKTFESFKVKEGDQIIFMVSKAKKVSEPVKEETSSSKIEQTPTASTATETSASNTNTSTEANAGTAETVTEFTASTFAQGSVRETAVQNIMAMGFEREQVERALRAAFNNPDRAVEYLLSDIPISTEPSAAPVSDSVPAAESTVDSTGETVPAAASTTPSEAPLVSTQGNLFDQAALASDGAGQGEVGGSEGDMMSQVRDILQAQPEMAEVVLQQLAASNPQIAQLIQTNPEAFLRYITEGDQQALAQALGVPPEYIEAAQNEEEGAEAGDSYQIQITPEENDAITRLCELGFDRNLVIQVYFACDKNEEMAANLLFNDHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTU5</accession>
    <name>A0A1E3NTU5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL23m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_14999</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020678.1">
        <property type="nucleotide sequence ID" value="XM_019160629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTU5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTU5"/>
    <dbReference type="GeneID" id="30177316"/>
    <dbReference type="OrthoDB" id="275582at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="250" mass="28986" checksum="47E091E66DFE9C28" modified="2017-01-18" version="1">MLPVRVLRSHATNSNSFQHISVRTIIMGYRKPFAPKPKPKESHDQRALRSKRIVQLVRDALENDEPNFVPGSKPIYLPFATIKLLRPNAKHTPYQAKFIVPKSFNKLDLRDYLYHIYGLRVLNITSALMPGKFIRSMPAPYGSRYREAQIKKMTVDLIDPFVWPAESEEYIQEKELTSELNRYREEKANAINSDAEKPSKAFGGIIDKQPRPMNFVPKVVKRQMKNTKDQDIIAKKKLEAEKFISSYIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTW3</accession>
    <name>A0A1E3NTW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glycosyltransferase family 71 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_29616</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020593.1">
        <property type="nucleotide sequence ID" value="XM_019162600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTW3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTW3"/>
    <dbReference type="GeneID" id="30179287"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="60865" checksum="7CEA3245FA14EF45" modified="2017-01-18" version="1">MFTELLPIITESAPKLGYQLQNEKDIGSRRGKFRLSTKIFPRTYGRNGVVNIAIHDTSEKFPILSKDFLNDCLYLPTDMFEVLKRSHEYFVSHIPDHYAPNTYSGEGIVFIGGGKFSWLSLLSIENLRSAGSKLPIEVMIPQPEEYEPQMCEIILPRLNAKCLLLYEIFPDIMSSEKTKKKDSTGFKLTGYQYKSLSLLASTFEKVLFLDSDNISVLNPDVLFHSEPFTSHGMILWPDFWRRVTHPMFYDLAGFNITERRVRNLMDKVTPPEVYTTGAEDPDTEVPLHDREGAIPDLSTESGQIIVDKKTHFKALLLSFYYNVYGPRHFYPLFSQGGNGEGDKETFIAASVFYNLPVYQMNKPVGVVGHWSNDDYEGVGMIQYDPIIDKLNEDHYKNEILRRINEEGDNFHYGKMDFFNYLNDAEARPMFFHSNYPKLDPISLFGENKLVDPDGNQWRMYSDQPDIGFDFELKQWQLINKYFCSVDEPFQMRYLEDSNVSMEKLCRAIESRLEFLRSTGLRFNYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTX8</accession>
    <name>A0A1E3NTX8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_101890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020559.1">
        <property type="nucleotide sequence ID" value="XM_019159645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTX8"/>
    <dbReference type="GeneID" id="30176332"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="122" mass="13087" checksum="1802C8627E20218F" modified="2017-01-18" version="1">MGKTSNGPWLTIWCCDTKSAREICREGWKLSSALAVGAPLKCAVLFPEDGLLILVTGAAADARIDNTAWGHPVLALHWVQKREANLNLNLLPLSLPPSSPSRSAFSRTANLSVPLAASPREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTZ7</accession>
    <name>A0A1E3NTZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial-processing peptidase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_71066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020633.1">
        <property type="nucleotide sequence ID" value="XM_019163817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTZ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTZ7"/>
    <dbReference type="GeneID" id="30180504"/>
    <dbReference type="OrthoDB" id="277191at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017087">
        <property type="term" value="C:mitochondrial processing peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="3">
        <location>
            <begin position="50"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="4">
        <location>
            <begin position="197"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="54537" checksum="044BC1B4C3D58C55" modified="2017-01-18" version="1">MLSHMRSARRIGARGLGARGLASAQAPASVSTPTVIKTLGSGVQVAIDPTPSYFSALGVYVTAGSRYEGTYGLTGCSHILDKLSFKSTANHSAKAMADALNRLGGNYMCASSREAMLYQASVFNPDVEHMFELLAQTVSEPRLSDEEISEQLISAEYELNEIWLQSDLILPELFQQVAYSNANLGCPLLCPQDKLSSLNRAQLLKYRDTFYRPENIVVAFSGVDEKTADQLVAKYLPYSGSSASPSQLIKEPARYTGGEFSLPVPEGIEYRGEEFHHIYVGFEGVDISSEDVYKLAVLQMLIGGGGSFSAGGPGKGMYSRAYTRVLNQYGFVESCKSFIHNFTDSGLFGLSLSCVPQADRVMAELIGYELNLLMSRDIGKGGISEHEVSRAKNQLKSSLMMNLESKMVQLEDMGRQVQIYGKRVDVLEMCAKINQVTRDDLIVMAEKIFTGSEPTIVVQGDREAFGDIRGMLARYGLGKTASASAEKKDNTTSRSRGWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUB2</accession>
    <name>A0A1E3NUB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional adapter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_14695</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020341.1">
        <property type="nucleotide sequence ID" value="XM_019160488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NUB2"/>
    <dbReference type="GeneID" id="30177175"/>
    <dbReference type="OrthoDB" id="270417at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071470">
        <property type="term" value="P:cellular response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010520">
        <property type="term" value="P:regulation of reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000008">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="14">
        <location>
            <begin position="59"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="64"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="13">
        <location>
            <begin position="344"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ49228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="432" mass="50636" checksum="95055D50F0430A24" modified="2017-01-18" version="1">MGKFHCDVCSSDCTRRIRISCAECQDYDLCVPCFAAGKVSGKHQPWHDYRVIEQHQYPIFDKDWGADEELLLIEGCQTLGVGNWQDVADYIEGRSKEEVGEHYEKYYLNSPYYPLPDLNQTFPEVSTTEFLKRRRQRFDGRKKMPLPPPKKILTSQPLCSHIQKYMPGRLEFEEEAEDEAEKVVQDMVFDTDEAEEDVELKLLILQIYNEKLSLRAERKRLMLKDDLLDYKSNNAIDKKRSKEERELYTRIKPFARIMSSSDFKEFSDDIMNEFKIRNRIMQLQNWRRNGITLLEDGEFYEKEKMSRVSKLTLSSPNISSKERHTLNSGRSHSRMSTPTDGKLKRRNISADPLDISDAPDYNLLSDGEKLLCQELRIYPKPYLCIKELLFKELLNSGGVLNQEKVGSIIKLEPSKTSRIYHYFVKQKWCQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVA0</accession>
    <name>A0A1E3NVA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020701.1">
        <property type="nucleotide sequence ID" value="XM_019163837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVA0"/>
    <dbReference type="GeneID" id="30180524"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="97" mass="11577" checksum="1265732451F52176" modified="2017-01-18" version="1">MVFVRRNTCGILRRRINKSNCTIRFLVIKSIGGNVWELLSFICSRIYIVSQFSRGDGISRRRFKIARRYRWVNNRGDTTRDHLAQCATVWWQQIDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND60</accession>
    <name>A0A1E3ND60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_18539</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015174.1">
        <property type="nucleotide sequence ID" value="XM_019162048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND60"/>
    <dbReference type="STRING" id="763406.A0A1E3ND60"/>
    <dbReference type="GeneID" id="30178735"/>
    <dbReference type="OrthoDB" id="6846267at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF8">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="6">
        <location>
            <begin position="19"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="543" mass="63303" checksum="9AD5A58985AF84A0" modified="2017-01-18" version="1">MGQTISTDNCLPYSLETPKNGKLSGITFNDPKTGKPACHRLAQVPYAYPCEGSTRFTLPKELPNDYDYTGEYKEFGLKCPQPSVPNPHFRYLKSPSKEVIQYSNIWIPASDKYKPEGGWPVLIYIHGGWLQYNTPNTDNFNVVEMFSDEEFMNKFILVVPGYRLNMFGFLSGKELLEENPLNSNFGFWDQRMAMEWTYKNINVFGGNPEKISVGGISAGSYSTFFQLAYEMYHPECLQIIKQCCFFSNLVYIQPKTIEESQDQFDEIIEKLNIDKTLSSHEKLEKLRELDTNFIEDFIPTLTLHTFRAVTDNHFISPKIIEDLNSGKFCQMMKDKKLRILNGEVDNERYKYSLLNTPTTIDELPIQVENYYPRSVVPTLLDLYEANPENFEGLSEPELKEKLRIKYGAIVGDGQVYSSARGLINKLVENGFPAEDIFRYRISFRAKWLDEHLEKDWKVPHGADLSVWFYNVRKGYTEKERTQLNKWLVPYLKFLNFEKQIEEWPTSDVKKIRLFREDGTEEYMQDPDWDWGVKVSNTVYKSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND77</accession>
    <name>A0A1E3ND77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketopantoate reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015187.1">
        <property type="nucleotide sequence ID" value="XM_019164909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND77"/>
    <dbReference type="STRING" id="763406.A0A1E3ND77"/>
    <dbReference type="GeneID" id="30181596"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="1">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="114" mass="12535" checksum="9958C625D1CFCC1B" modified="2017-01-18" version="1" fragment="single">DVFSRVDSTGPGEGVKVAKADGVELPEDVINTVVHSDDGDWFKPSMLVDVEKGNPIELEVILGNLLVVARELKVETPTLDLLYHLLKATQFKLKEGKGLIDVPKERSISSKFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDN0</accession>
    <name>A0A1E3NDN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab proteins geranylgeranyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74846</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015352.1">
        <property type="nucleotide sequence ID" value="XM_019164892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDN0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDN0"/>
    <dbReference type="GeneID" id="30181579"/>
    <dbReference type="OrthoDB" id="1923006at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017230">
        <property type="entry name" value="Mrs6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037514">
        <property type="entry name" value="Rab_ger_ger_transf_A_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00894">
        <property type="entry name" value="YEASTMRS6P"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="611" mass="69178" checksum="3A641D8960B674F0" modified="2017-01-18" version="1">MTSYTDRRPSIVERRPSSVSAFSSRRPSVIPELQGIEKPNMESLPDDLECDVLICGTGLIESILAAALSWQGSKVLHIDSNQYYGDNSATLTIEQIKSWFINSKMQQTGFKNYKNIKLDINDHDFKIVSRDYGIDLSPKILFTKSNLLSLLIKSRVYHYLEFQSLSDFHTFEDNSFGKLVSSKEDIFTDESLNLLAKRKLMKFLKFIVGDDYLAAIKEEDKVLTLKEYLSKTFKLEDKFSNELIYTLGLVNNPNIDTISGLERIRRYLLSLNVYGNFPTVYSKFGGPGEISQGFCRSAAVAGTIYKLNTKLVDYDAESKVANLNDGSKIKINERVVSSLNQSKEKLESLGKKLHEISRMVIIVDKPCCEWFNEGESASVVVFPPKSLNSNNEYPVECLIMGAGTGCCPDGKCIWYLHTIAPTTNSVAEHDLNCALKKMEESILRESNFDFEDFDEGDLTMVNENKLGLTPSGTLKLSQSLQNFKAKEKLRYVMKLEFTQLTIVDQAESFRGYGYSDEIATTKDGIIISNMPSSELSYDGIIEEAKKLYREIVGDDVDFFDVDFEDDEDEKEMNDFNNMKTAMDMDMEMDDNEQAILDESEEGQSGDLEFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDT2</accession>
    <name>A0A1E3NDT2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015400.1">
        <property type="nucleotide sequence ID" value="XM_019161914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDT2"/>
    <dbReference type="GeneID" id="30178601"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="101" mass="11125" checksum="FD1276264BFD5C1D" modified="2017-01-18" version="1">MAANFPVLDYSRCLIMIRLLSLITQSLLASASYLNTKITYHDKILISCPSIMIGFGILSQHHVLTFTSNHIRSAASQSKNGKNMQLVVLKGLAALNIPRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDX7</accession>
    <name>A0A1E3NDX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74588</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015450.1">
        <property type="nucleotide sequence ID" value="XM_019164809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDX7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDX7"/>
    <dbReference type="GeneID" id="30181496"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25523" checksum="54BFD54D84CD6041" modified="2017-01-18" version="1">MENQDSSVIRVFVVRHGQTAWNIEKILQGHQDIALNETGHSQARLLGSRFKDIDFDMWISSDLKRCIQTTQGIEAAVGADKVPGQFVKSPAFRERFMGDVEGMKIGEAKAKYGDGFRNRGEKKEDMIARIYKRWLECLAMCAQEGYRNVLLCTHGGVIRNFVNYLYHEQGYQLGPGMTAADLRVPYNTSVTVLDIKRADICAGTIERFGCTEHLGEQKEVGDKDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDZ9</accession>
    <name>A0A1E3NDZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">H/ACA ribonucleoprotein complex subunit NOP10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Nucleolar protein family A member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">snoRNP protein NOP10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_37292</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015465.1">
        <property type="nucleotide sequence ID" value="XM_019163074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NDZ9"/>
    <dbReference type="GeneID" id="30179761"/>
    <dbReference type="OrthoDB" id="13807at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904874">
        <property type="term" value="P:positive regulation of telomerase RNA localization to Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.300:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="57" mass="6652" checksum="C5FF9F162D2FBA25" modified="2017-01-18" version="1">MHLMYTLGPDGKRIYTLKKVTESGEITKSAHPARFSPDDKFSRQRVTLKKRFNMLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE48</accession>
    <name>A0A1E3NE48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_18320</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 15">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015524.1">
        <property type="nucleotide sequence ID" value="XM_019161950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE48"/>
    <dbReference type="STRING" id="763406.A0A1E3NE48"/>
    <dbReference type="GeneID" id="30178637"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000008">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="16">
        <location>
            <begin position="50"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="17">
        <location>
            <begin position="249"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ44411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="446" mass="49556" checksum="4A042A883A76266A" modified="2017-01-18" version="1">MREVISVNVGQAGCQIGNSCWELYSMEHGITPDGFLEEGLTKPRGGEEGFSTFFSETGAGKYVPRALYVDLEPNVIDEVRTGKYKDLFHPEQLINGKEDAANNYARGHYTIGRELLDDVDDRIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLEQFSNDYGKKAKLEFAVYPSPKVNASVVEPYNTVLTTHTTLENADCTFMVDNEAIYDICRKNLGIARPSFSNLNALIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPVISKDSANHEANSVNEITAACFEPGNQMVKCDPRNGKYMATCLLYRGDVVSRDVMNAVSIAKSKKSMQMVDWCPTGFKIGICYEKPTQSPESQMADVNRAVCMLSNTTAIADAWKKIDHKFDLMYKKRAFVHWYVSEGMEEGEFAEAREDLAALEKDYMELGADSFVEDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEE2</accession>
    <name>A0A1E3NEE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74726</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015607.1">
        <property type="nucleotide sequence ID" value="XM_019164849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEE2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEE2"/>
    <dbReference type="GeneID" id="30181536"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="SPRY" evidence="3">
        <location>
            <begin position="219"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="557" mass="62170" checksum="E736AD2FE4D65E17" modified="2017-01-18" version="1">MDDNVFLFFLFGMTAVVFFSLILISYLVFYFVFQDDESETPEGHLPSQMRLDLARYSKLDNYSRFMKMSDTEKITYLLSVSYCRFNKPNLVTTELSSTKVTTESLLIRDRGINSFHFLDYYDQIGSLVSKLENEQSENSEMTDGDVDETTALLENTELDFKNNKILKLLKAYNYKSCISKSIPFVVEDLVEINFKPTLINGISYSTLLNLPIPTVNRKNDVIYFETKLLEFSTLSTLVSIGLVTNPNYPNFQLPGYLPYSFAIDSTGNLRITKKQTYEPDSSAEDELNIVLPQLTEGDVIGLGYRSISGTIFLTHNGKLIHEVVKYFKFQMFPCIGFKNLATLDKKSNNGCKINVNLGQMGFVYIEANVKKLGFCENRNDGLIGAPPIYNKTNQTNEILLDKGDDIPPEYPTDEDTFFGPVVGSSTAEKLDNEAVTTTTEDKKVADLEEGNAADNSSASSRRTGTPASEPPSYQSEKSGDKSNMTDQEYESFVSPVEAQETGTTTEILPESDGATANGETTPLIPQQVLSGGKAKASVGSKQKLKKHKKKKKSKTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEK5</accession>
    <name>A0A1E3NEK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydrophilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015665.1">
        <property type="nucleotide sequence ID" value="XM_019160031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEK5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEK5"/>
    <dbReference type="GeneID" id="30176718"/>
    <dbReference type="OrthoDB" id="3996978at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042631">
        <property type="term" value="P:cellular response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133331" description="Hydrophilin" evidence="2">
        <location>
            <begin position="19"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="21962" checksum="C254C2E7B6BDE1CA" modified="2017-01-18" version="1">MKFSTVLTTALLVSASNAAALPEAEADAKTPTTTVTTTHTGPTTHKYGRFNNTHHETTKTTSTGTHKYGRFNNTHHETTKTTSTGTHKYGRFNNTHHETTKTTSTGTHKYGRFNNTHHETTKTTSTGTHKYGRFNNTHHETTKSTSTGTHKFGKFNNTHHDVATTTVYEADAAPAVASPFIMKNSVIGWSVGAVGCAAAALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEN2</accession>
    <name>A0A1E3NEN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial phosphate carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_36530</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015720.1">
        <property type="nucleotide sequence ID" value="XM_019163014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEN2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEN2"/>
    <dbReference type="GeneID" id="30179701"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="13"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="111"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="211"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="32611" checksum="6F2CCD07ACB46C15" modified="2017-01-18" version="1">MSTAPVIPNFTVTDYSKFALAGAIGCGITHGSMTPVDVVKTRIQLEPTVYNSGMFGSFKKIISEEGAGALLTGLGPTVLGYSIQGAFKFGGYELFKKVFIDSLGYETASNYKDAVYIGSAAIAEFFADIALCPLEATRIRLVSQPTFANGLIGGFSRILKEEGVGSFYAGFTPILFKQIPYNIAKFLTYERAAEKIYDLVATPKDQLSSLAVTSINLGAGVIAGCMAAFVSQPADTLLSKVNKTKKAPGQSTVGLLFQLAKELGVLGSFAGLPTRLVMVGTLTSLQFALYGSIKGYLGCPPVVSLGGASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEP1</accession>
    <name>A0A1E3NEP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rrn7/TAF1B C-terminal cyclin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_60276</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRN7/TAF1B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015707.1">
        <property type="nucleotide sequence ID" value="XM_019163422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NEP1"/>
    <dbReference type="GeneID" id="30180109"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048538">
        <property type="entry name" value="Rrn7_cyclin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033599">
        <property type="entry name" value="TAF1B/Rrn7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576:SF2">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20645">
        <property type="entry name" value="Rrn7_cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Rrn7/TAF1B C-terminal cyclin" evidence="10">
        <location>
            <begin position="221"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="502" mass="57440" checksum="C5E5443570D54A65" modified="2017-01-18" version="1">MARYVRGQVCGVDNCPSRLWKRVNGQNVCQYGHVNQFDIEIDDDDEMMNVGGGAGGAGGGGGPDFSRRLVNVAGLTSSQAMRNRVNRLSSDKVETRKYGDDFKRLQARCFQMILAKNTRFLAQQLALNSEQRRLYTSVVKLIWVQLLDSSLNRRLDTKHRAYDIGHLSVVNYLAALQLNVPLYLCDYIAATFDKSFHLERAEYCLPRDLRIQIPISQVSTFHGHTLYNYLKRLNGRFASQYIAAHVTESRLNYFPLLVRVVLNLFLPLGVVKLVKNCVDLLGLDFAFHRLPETRIHPELQLMALLIIFADVYFANNPENEYADWYLRLAKNTASGARSPSFVDFKKKLFFNSSFEDLYRWSDAQVDEFVDFYDTNVLPSISSSNVASNNEYKDRNKLQIVKSTQELFDKELVAAHDDYAHTPLPPAQQFEQHTAMLLDTYRERHGHGHGHNHTPPPKDPFLLETLFRHITSMYNCSDLQLKDALRKVWRQVRPAGEGQGPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NER1</accession>
    <name>A0A1E3NER1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_13262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015699.1">
        <property type="nucleotide sequence ID" value="XM_019160036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NER1"/>
    <dbReference type="GeneID" id="30176723"/>
    <dbReference type="OrthoDB" id="73691at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="44218" checksum="A6EE6D89694E44DF" modified="2017-01-18" version="1">MIFPVHTATRNVGVGVGVGVRSAQRPAPRAAVWARRCYSADNVHSKADNDYLLLQAIRGDAAARAPDRDDLLNSPTTLPRYDLLKTVGQIRRELLAGRPGPSPARAYDGLVVQYRRARNVFRFFKEGLLNVWRVHRALRRGLFSGRRYVVDYSEAGGGRVAVQRGDFGRVVDELSGRVALLKIEYDAGRRTAAGFLGRPRPELLLSRREFVEMVRDRANFVKLPLFGALFVVLEELSLPLIFLFPQMLPGTCVLPGALERRYYARSARAFGRLVAQHSRSESECRDFVQAVAMGRVSAAHHELADARTRRDVCHVLHCRGTTAAALQQHQKILLADDYLVVAGGGVGRLSDAEVVHGCVVRGLAAGQAVGGAAVGAGGADADAVAGLRQQLGRWLDVRFVQRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEV0</accession>
    <name>A0A1E3NEV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015762.1">
        <property type="nucleotide sequence ID" value="XM_019164748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEV0"/>
    <dbReference type="STRING" id="763406.A0A1E3NEV0"/>
    <dbReference type="GeneID" id="30181435"/>
    <dbReference type="OrthoDB" id="10262413at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF6">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="344" mass="38811" checksum="C574826A6CF1333D" modified="2017-01-18" version="1">MYEKKLFITGATGYIGGEILHQILQSFPTFEVTALVRSVEKGELIEKRTERKVKTVIGSLDSLDLIRKETELSDVVINTASNNHLASLEVIKSVLSKKTSETLFIQISGTGVITDSMDPEKYTPDKVYDDVKDIEEINSLDDSQPHRQADKLTLSIEETNPQFVKTAIVSPPTIFGISNGYDRKYSIQVPLLAIETVKVGYSFTVFKGDTRWSHVHIYDVGSLYVSIIKKFIEKEDFRSGHFGYYFTNDGTDFTWKEITTRVVEALYSRKLIKSKEIRELSPKEVDEIFHLPPLFWGANARTKAEAGRLLGWIPVKSTDSDFLSAIDTSIEYLEKHGLLGPGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF35</accession>
    <name>A0A1E3NF35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BolA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_36858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015878.1">
        <property type="nucleotide sequence ID" value="XM_019163040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF35"/>
    <dbReference type="STRING" id="763406.A0A1E3NF35"/>
    <dbReference type="GeneID" id="30179727"/>
    <dbReference type="OrthoDB" id="4983at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071281">
        <property type="term" value="P:cellular response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045115">
        <property type="entry name" value="BOL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12735:SF27">
        <property type="entry name" value="BOLA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12735">
        <property type="entry name" value="BOLA-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9937" checksum="AC3D9F89CD241606" modified="2017-01-18" version="1">MPLTSDDLTQIIKNRLQATEVEVQDMSGGCGQAYAVIIVSDIFLGKNKLARHRMVNSALKEEISQIHAFTQKVFTVAEWDEQKRLFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF82</accession>
    <name>A0A1E3NF82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">STB6-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015903.1">
        <property type="nucleotide sequence ID" value="XM_019164782.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NF82"/>
    <dbReference type="GeneID" id="30181469"/>
    <dbReference type="OrthoDB" id="19806at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038919">
        <property type="entry name" value="STB2/STB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059025">
        <property type="entry name" value="STB6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011:SF2">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25995">
        <property type="entry name" value="STB6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STB6-like N-terminal" evidence="2">
        <location>
            <begin position="56"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="644"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="759"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="616"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="759"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1339" mass="152540" checksum="2C571217D2013C6D" modified="2017-01-18" version="1">MSFRSESQTQPRSRAGSNPWVQYSDFFTNNSFLIPDTIAFAKILPYFKVLPGLTNRFSIRLVELVGYEAYFVEQWISSRSSDNLIVTYTGDQKDRLIAYYVQSLSEHMQLQPPPQVHEPSKSLTQASKQHLPYDNWPPKFISYLVEQLESPYCVATETSLGYAFITNLTQLNPFLSLIAAKTGNIADDYNCYVVNYNLRKLGCGSRSATTTDEPSKSMEDKFRAFFKVDPKVPINYSAVGMILVTQTFLYYYGLLDPLYCDGLFCERTEAAIMDWWKLISEIPMALHVIRMKPPSSHPADSIQAIIGFTVLCRYLLELGGNNFNVPKDPMDVKKFKAAISKFQKHSKIDSTSKFNLETLLKLMDWGQNNKVSQNLTKDLSKMKNLVKNTVIDITSGKNLQIIAHNATASPFYLHSHSSYDHSKLINCQNIDHIKHMSLGKQLSYLFFASGKPVDFNKDSLSVQANKKVADSTNNLLIDGVKRLKSQLSIQSLQNPKSHNNFLDIKGLSATTLNLFDKDHDQEADVDLSNDRHSNIEIDEVLSNSNEEYNDTAVRPQKKKKRSASSVLFSSEDDSPDDKIENVRSTSLADFDFDDYNDDVAVYNPTRSRINSNTNKANSIGTAASAGSGSDNGYDNYADYDGDYTNYYNNDDDDPYYNHNSSKVNGHGNRGSSFSSHVRFPRKNVVDMNNIDEALIDDGFSDSELYNNKSTLNSAKNSDSVRESRALLNGELKTLYTPPRIISSGELSRPNSMLNSVNKTLSNRQHLSRGSSGNSYDSDGIYEYENGFEYDYELDPNHHQNNHTGIHPHSRKHSYLPSSKEHSRRPSSLGLNNAPNGSEKRENIGQDNTAFSTQTNLSSSNGRNESCDYSTSNVDFEYCNFMKRIKRRHSIPIVESEINKYSIEMRMKMKRDKAENLINNNLERKVSWSVGRRNMWDRTSMNSMDTDDDLSLKSNNQQSLLHDLTPDLNSVNNHELNSLSKLHGKETEMSDQNGSNFLYRMKRKQSIFGAQSVYTNGSNKSSYAPMDTFIIYHNPSSSARLRRSQSFSGIESSLMCSGKGTSVSGFEFSDVSFLTSEVLAMKYLKLEASYQIEIVQGSFNLTKDLQSYSRYLLESINSNPNTCVSLMYNTTRGDVKKVIGKYFELEDKLKNTVKSNARLKYELRLLLQKTKEVENNLKTLQDFKIKTLNSKIESMILELAKYGPMDHELKELSMEKTLSKAENLSSDAEKPILDRIYADDGNLNWSKISIRNIWNNPYILIYLLFHFLLCTVLKRVDAKMIEQRWKEIDKNQTVTMIIRKLYSKSERELHKEEEEEKKKNAIWKTDSEKTIHSIKKKASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFF3</accession>
    <name>A0A1E3NFF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dethiobiotin synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015166.1">
        <property type="nucleotide sequence ID" value="XM_019162043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFF3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFF3"/>
    <dbReference type="GeneID" id="30178730"/>
    <dbReference type="OrthoDB" id="425114at2759"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03109">
        <property type="entry name" value="DTBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210:SF5">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13500">
        <property type="entry name" value="AAA_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="23574" checksum="EE9FD6FAD128AF85" modified="2017-01-18" version="1">MTPRVFVAGTDTDIGKTFISALTVKKWEFDYWKPIQTGLSEDQGDTKTVKQLLRESGGIPRTSNIFEPTLTYMKPLSPWRCTVLEKQSSINVESIEIPVSENERALNGMIIEGAGGLLVPISKNSFTTDLIRKLDASVILVARSELGTLNHTLMSLEILKQNKIPVIGVILNGALNGDNAAALSELGVRIIAQVPIVKSLDEAIDYIPTIDLLTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFM8</accession>
    <name>A0A1E3NFM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_12684</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016042.1">
        <property type="nucleotide sequence ID" value="XM_019159958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFM8"/>
    <dbReference type="GeneID" id="30176645"/>
    <dbReference type="OrthoDB" id="10259639at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="394" mass="42950" checksum="47B74F0F551D0440" modified="2017-01-18" version="1">MSQLPGPTPVSSSSASSSSSSSSSSSSPFPTTSSPPSPFKLKAVLADTSLCDYLNGVLNTILFVRLLGLLEPLENVPLSQHSFQNTVYPVISDTSSLRHHSGDDDYLLNRSPREWVASKVEALAKSYREKTHSKRKSQPPTLADLYTKVIIVLRLYNAGHQHATGKNHTGNTAPAGKCWEAWIIDLDVLGNTETTATTTHTNINNTPVAHTIDRTTTVSTTGTAVASTGKERAKDTINLETLLRFSSMDSESRTDAMQTSFRSNMFHIIECADSNCDSIPSVRTQSLNPFLIKTEFQIKPNMYSNNKNDNDTSNNLPGELELDFNSLNISSITGDSGLSHESIEYINTTENGDVVISSGSGSGSGSKSDNDTYANKDKEDLWKNGYNFFKKMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFR1</accession>
    <name>A0A1E3NFR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015296.1">
        <property type="nucleotide sequence ID" value="XM_019160102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFR1"/>
    <dbReference type="STRING" id="763406.A0A1E3NFR1"/>
    <dbReference type="GeneID" id="30176789"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="130" mass="13901" checksum="0F9E4E35B7C6924B" modified="2017-01-18" version="1">MVTRVTWEQLNKAGNNLLSPSSVSNGRVFTSGCLGTDAEGKMPESVAEQTKNAIESLGEVLKASGSSFDDVVKVLLFVGDASYVSEVNQIYSKYFTLRPARSCVVVGFPNPHVKVEIECVAEVSSLAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFU2</accession>
    <name>A0A1E3NFU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_158232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016120.1">
        <property type="nucleotide sequence ID" value="XM_019160981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFU2"/>
    <dbReference type="GeneID" id="30177668"/>
    <dbReference type="OrthoDB" id="5945798at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF251">
        <property type="entry name" value="EG:BACR7C10.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="479" mass="55427" checksum="69984B593DCCB242" modified="2017-01-18" version="1">MDHFNNNLGLMFDPIFSMINHSCDPNCTLIWRDDGEILIKSVKRLRASNEILLNYIPVHLPREMRKKMLRNSFFFDCECLKCTVSDKDYDAMLPINCLFCNHNNRGFVLENFEKFESVPDIGKKKMCNNCENIIDVDEIFQNYLDIFRFFRSLNKEAEKLQDFCSWTLESENVMNMDTQTFRNGVKLLQKSYEIVPLKSWPMITLLNVVKIGFQFRDPFDIKVLRMTYLTNFLTENMLESEYRLKVDIGASLYDLSVVSADYLFDQYLRYKEELDEEILDAVGKGSFSLCILSYKHLQERFSDSNDSESEKSMERSQKDMVQLALEIRRLLEHFERRKRGEKNRKGSKASNRNNSTVSSQNKNLVLRSTATAGAENGNYFDNYTQKVFEESIINYERYMECGAATQFKPILLSLTTPDYKTFSLKVPRSLGSKNGLDKGVGKGSGTCVKTDQELIGESKVAGSPSPFVARPTWVAQLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFX1</accession>
    <name>A0A1E3NFX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glyoxylate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016137.1">
        <property type="nucleotide sequence ID" value="XM_019164599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NFX1"/>
    <dbReference type="GeneID" id="30181286"/>
    <dbReference type="OrthoDB" id="9991913at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12168">
        <property type="entry name" value="Mand_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF257">
        <property type="entry name" value="GLYOXYLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="74"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="137"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39087" checksum="F04324E752A13A0D" modified="2017-01-18" version="1">MFLKRVCRNYSTGKSLTVLRLGPTRFAQQAWSNLVSKNNVKVVETDAANREQFISELKSGKFADVDFITRTFESFKQTGMMDKQLLKLIKEHTKVKAMSHNGAGYDQVDAIECGNLKIQLSNVPSLVDDATADTAVYLLLGAMRNFQLSSENMKKGQWKTQKCAGTPIGHDPEGKVLGILGMGGIGRTIRDRLVPFGFSKILYHNRHQLPKELEKGAIYCKTPEELFKESDVISISIPLNKNTKHIINEKSIGIMKDGVVIVNTARGPVIDESALKTALKSGKVAAFGADVWVNEPNVDPELISLSNVACLPHMGTHTVETMKAMEEFVVENVQTYIDTNKVLTMVPELKGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGE4</accession>
    <name>A0A1E3NGE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Translation associated element 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_17696</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016320.1">
        <property type="nucleotide sequence ID" value="XM_019161700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGE4"/>
    <dbReference type="STRING" id="763406.A0A1E3NGE4"/>
    <dbReference type="GeneID" id="30178387"/>
    <dbReference type="OrthoDB" id="1298661at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000025">
        <property type="entry name" value="N-terminal Xaa-Pro-Lys N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="246" mass="27947" checksum="EA27EE298BF16966" modified="2017-01-18" version="1">MPGKIVAENAETGGEEENVDKLINYNDAISYWTDVPATVDGVLGGFGEATSVPRADVVGSSTFMRKLKTRWSAEPGKIKYGIDFGAGIGRVTRDFLHKVCDKVDLLEPVEPFVQQMRVELEPLRKEGKIGEIYDIPMQDWKAEEGRYALIWCQWCCGHLPDDAFLEWLNQCKRGLQQDGLLIVKENNTNSDDDLFDPEDSSKTRSDKNFRRLFKQAGWKLIATDLQKGVPRELFPIRMYALKPTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGJ5</accession>
    <name>A0A1E3NGJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015576.1">
        <property type="nucleotide sequence ID" value="XM_019161968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGJ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGJ5"/>
    <dbReference type="GeneID" id="30178655"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="226" mass="26216" checksum="2821BC214C5C056F" modified="2017-01-18" version="1">MDASDHFKLMQEALKYGEVALSSDEVPVAAILVDNSTKKILYRAHNMTNITLNGTAHAEFRIYDHLKCSYPKDHLEMWHNCTLYVTVEPCIMCASMLDQIGVSMIVFGCPNERFGGNGSVFDIRYKSNIKLVPGVGHREAISLLRRFYIRENERSPQNINKKKRTLKLDEFPQFKYSQFITLPEFIDIWGKDAEQIYLENDFLEFEESGKLILPRKVDTDKKRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGP3</accession>
    <name>A0A1E3NGP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein HSM3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016438.1">
        <property type="nucleotide sequence ID" value="XM_019164675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGP3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGP3"/>
    <dbReference type="GeneID" id="30181362"/>
    <dbReference type="OrthoDB" id="4074002at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041335">
        <property type="entry name" value="HSM3_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18795">
        <property type="entry name" value="HSM3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein HSM3 N-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="477" mass="55310" checksum="7E04DFD4433DE00A" modified="2017-01-18" version="1">MTIADPVVQQFAEHLNELYDDPYVEPDNLLINELEDKIRYENLVQHDSSILVQLAPTLLRIINSNNTTGITKNYAIKFLDILLPHYSFNQILSVFSEDLLVKAFQGNDNLKRVIAKTLIKADPAKVSHGPLFLCLFKTFANPSTELSTINEVQKAIVALTLESDEIRQMYLNNTEIVQILEEMKHNKVVQSRVIDLVCDILIVIPQLPTSIYLVGEEELARSNDLLFYRFCVGTWRTLLHLIHQDDSLRFLKEKLRPQIDFGSRRFVGAQTLLDDKDIFVDYDDFGIVYFLVTLSFVFPDFFKEFDERYHLVDYAVETYRHYLKSVNFLASLNTIFLRNNEGLYRNFKLCNNSVKLFCHLLNDKIILEQKLTLHEFPPSIFDKLVFDDLFTIFGTLCDSEWKIEKMVNDWSNIIEKVIDVDIVNDVNINSHHLESFLKAIAKSGIPLGRLEYPIDEKLEKLKKQTGVSVEEPLTELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGS5</accession>
    <name>A0A1E3NGS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clock-controlled protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016444.1">
        <property type="nucleotide sequence ID" value="XM_019164677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGS5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGS5"/>
    <dbReference type="GeneID" id="30181364"/>
    <dbReference type="OrthoDB" id="4094614at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038843">
        <property type="entry name" value="Sed1/Spi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35523">
        <property type="entry name" value="CELL WALL PROTEIN SED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35523:SF1">
        <property type="entry name" value="CELL WALL PROTEIN SED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133360" description="Clock-controlled protein 6" evidence="1">
        <location>
            <begin position="18"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="10721" checksum="344BE6CAEC0348AD" modified="2017-01-18" version="1">MQFKFLAPLALAGSAVAGFSNDTTVTEVVTALTTYCPEATTIVTNGKTYTVTSATTLTITDCPCTIVHTTSVAPSTAPKSSASVFTDGAAKIGVAGLAAAAGAAVYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGZ0</accession>
    <name>A0A1E3NGZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PITH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18028</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015726.1">
        <property type="nucleotide sequence ID" value="XM_019161853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGZ0"/>
    <dbReference type="GeneID" id="30178540"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="3">
        <location>
            <begin position="27"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="243" mass="26084" checksum="AA6A65B00FEDAE38" modified="2017-01-18" version="1">MNCADEASEDDHHTHSHSHSHTHPHGDPPVATTVAQSLNHRIDTPRLAAYNLRNPQHELPRLFKTQAARLETTPVFRSHFGPELLITIPFTGPVKLFSLLVRAAPDAPHAPHTLRLYRNAPQLSLDSVRAARPTYVCEHPAAASAAASAADSAADTAGFVEHHLPRRKFAGTSTLTIFFDPGPAADPDAGPALELYALELRGDWAGPATRAPVALLYESAARREDHPVGQTADAAASSSRLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGZ1</accession>
    <name>A0A1E3NGZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016514.1">
        <property type="nucleotide sequence ID" value="XM_019164693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGZ1"/>
    <dbReference type="GeneID" id="30181380"/>
    <dbReference type="OrthoDB" id="10444822at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="2">
        <location>
            <begin position="148"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="284" mass="33010" checksum="94E59FFA4803E8CF" modified="2017-01-18" version="1">MDLGQIDDIVYRLKAVTLKDNENDKVFEEKENSVVSEKGKKQRPAARQGYVSTEDADIPSVDEELAAEYYRTVKDFGKVEFWKMSAKSNHIESRLNPQKFHHHILQPAYLQDNKEKYHFKINLNCPDSATDSELENYNLMRKKSIKENAMTHRFSRHSKSDHTHIEYLCRMCSFKRWVPMKRFEEHMCLAHGILNVSGFGYVGLPPPDAVYRQSPGKLKCYYSKCPKCLRWIRLGKVAGVGRVSRKVRLHDSINNDQVVVGLYTNYFQHFLACSQEEEGPAEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH07</accession>
    <name>A0A1E3NH07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4536 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016543.1">
        <property type="nucleotide sequence ID" value="XM_019164701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH07"/>
    <dbReference type="GeneID" id="30181388"/>
    <dbReference type="OrthoDB" id="4083608at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053092">
        <property type="entry name" value="Mitochondrial_unc_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28048">
        <property type="entry name" value="ACR195WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28048:SF1">
        <property type="entry name" value="ACR195WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="10880" checksum="4099131281A608E9" modified="2017-01-18" version="1">MIPSNILGVLTPAEGSRKIPDEEKCLACQLMGSALCLAGGIYFMSEIPFQGSNASTKKNPLWWKNSVKSAGLGLFGYGIYRGGEGWLWNKDIKYKDTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH09</accession>
    <name>A0A1E3NH09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HECT-type E3 ubiquitin transferase TOM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17870</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016512.1">
        <property type="nucleotide sequence ID" value="XM_019161785.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NH09"/>
    <dbReference type="GeneID" id="30178472"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010309">
        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010314">
        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06012">
        <property type="entry name" value="DUF908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06025">
        <property type="entry name" value="DUF913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="14">
        <location>
            <begin position="3041"/>
            <end position="3377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2020"/>
            <end position="2089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2120"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2330"/>
            <end position="2383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2456"/>
            <end position="2509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2032"/>
            <end position="2074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2330"/>
            <end position="2367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="2368"/>
            <end position="2378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2462"/>
            <end position="2477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2485"/>
            <end position="2509"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="3344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="3377" mass="385259" checksum="C42B418318439D66" modified="2017-01-18" version="1">MLIQLKNSIDPEEAIPDNLKAHIDYLTNCPIEDLIKKLSTLYPKDWDGTRPNLKHYVSLLNRIDKIYESHIERYRLNEDYIAPLDVPHDEVEIITTCVNYSNQLLEYSTSRDIYNSHGYIADLMFSNSLDIKISALKLTCCLSEKFASHYPMKFTLSKKHRDLLVDFIKRFPLQSIRTTPGPTPIEVKQYEQQAGVVSKAPSVTPEQKTKKLKTKDSDKNIIHISLYDCIRPDFVTPSSWKILNYEYYKTGSVIKSDKQESSRHGKKKKLEKDKTGEGLRTFKLSSESLKKLSMQQIFDKAANVIPKEKWCDFVLHVYIAIAYSGKSFECLALRGKLVTFKCYSVAAAASNITYGTFVGLVFDEEPYLLSYMCDLVNPDNHVPREPCIATLRAFVNISNRKNGASDLMRALGGNVSHGLLFHILKAILKQTKEHKFNNDQTYMNYIYNILANLLENKSLAIHLRAAGLMKIFLDFLSLRNNYRMTRSGPLHLIEIFIERLSDAFEEFVTNDGFNVLISLLEFEVEFAIDNPDYEGGAPKDSNLSHIITARQVKLLNFLLRLVNSLISTYPGDRMRNLYDSPMLKSIIKIMQNPKIFGYELLFDCIRIITTIINSEPTAYSILNEAGVIDTFFNRFSTFLLPDSELLLELPDVINAISLNTGGLTKVKETKVISTLFSIFTNIEICKQLVELENVMSLGHAIDELARHHPELKDVINEEVLKLINIIPDSVQFDAVDFYQSPNGSLYRNQSDENIHTETSSPLLERWESSDKASVIQCTLIFLASMFENSKEWKRLYGHIDMNNLFKFITLKNAPFDYSLSKTIIHFRNIIKWIDQTTRSYCLPFLKERLASTLSKLHDFTYYSNDNNSFFLQFENADDVGKETAREVISNLGIMNCLLFVLSDFYSKLHKYQPNKILDIAQAFGSDDGLNLIGELCLFYRRLAIEEVLLHVYTPPSVAQNAFTLVQSISPKQKEIGVPPSDSCDWNGNSAKFKNISIMYFNFSRSKFWLRNVFNSFCSINWGRRSESKTLFGVSARYAVGIINQYTSVTLNMLTRINTSNDQIKYGYLFCMLNQLYLNVFHSFGVSAAVNPTMSICLLQNNKFPLLKDLAVDLFSRLGTFEKSKIDYFVEKPYISIELESLVPAVLDQILNLHADVGAVNVIGTLPNAHKLYKYLGPEKSEYGSEVLVSIQVQAAIADFILLQEITSTNRLDILNRFPTKIPVSLIEDIILLSKCAFSSFKSHSLTFKGRLYPIAAETTAPSDYNINFLANLGIPKDAAKEILLFFGDDIPKLLKETPENLDETFGILENVNWEDVLKQKYVPPLVESIHLNYSPNYDIDTIDDLYFHRGAEEVNFISYWIKIAQLYPDSASGVADLLFSVFGNSFSESMEDVLDPLFAVVKGMDFNSEDETENQKLSSTLKLFGYIVGRLSLPKYFSLIENFAKFLSQHMIENNVEKPWFASALTIFSKLFSETKIPFAPQAPKVNVPSKFSPYLITHPDIFSISPESEASLLNLLLNLKVIQTEEVAIEVANILLMLCTDDERVKMLAGAKIMHSLVRFIKKEHCSVRLQSQVINILRRSVESEYIVKSYFEKDINQIFSAKSKKNKVKDLKIFLEENSALVLRNPSIFCEVVGENCIVKRINRHFKSPLVSILDDEEKEILKKHGVDVNQTHDHPDENTSEIMSFLLSELITISRKDLMVGVQKDDNESKNDKDADDINTLVSNNNSLAYAVFLLQSVSELLFSYTSCKTDFLTFSKKEKNKSDKKPRSTALNMLVHRFITINPFEKEETSSHKVHQLLASLGYACILGLISTVPSKGAEYINTSVSDSDVAFARKFTADSLIKVIKDTEQSNKSSIIKYGRIVDILNLIRKLCGESFNNAVSVSTDPFITKNDKYYFAKELLEKKIGNVATNILAKLDTNFPYTEQVADSVLKLISLLGEIKVEYQDAFKADQQSTDVEEEVFDEELEYKDDAPDLLKNSTLGMYDLEDVEDEDDFDDDFDDDFNDEFLVDDGIEIILSDNDRGSGSDIDDDMDSDNDDSMEEGSDDDDQDHGNDSDDTENMEGVGDDEMGNPSYDNHGLHDEQIDFVYDSEDSDNSDDAMMYEHEIDDYVSNEEGDLVSGDEMSDDEHFMRGDSSGSDSETVDDSGENVIELDLESIDENDDDSGSDSDDSVILQEWLDELENDHGRSGQRSRRPSVVSLNANHNDQMFPTGILFPQMGSNIPLPTESAAGNDTRSTLLEFTQSLFDRRNNNQIQGLLDFRRVFEPLIFSKRASQLNSVLIKSTAQRWQETSELYSGKNITYRAIPEIINRLYNKSEIVNEEYKRVKEEKERKQREKQEEEKKKHNEWLQKERNLEFEREQDSSNNAETSNEPSEPVYVEIGGRDVDISGTGIDPEFLLALPDVMRQEVYEQQVHQARIEDRGREAIGFIRNLSLGSNQFNFGFGAGANSDNGLINGDDDNDEDDDDDEDGNEGFGYGLENDDDDENDVDDDDDDVDDDVDDSEFGLRLRDERGSVEMQRTEVPTGENSATEKSRKPSKIYFTALVDKPGIAALLKLLFIPQVYYKRELFFKAIAYLCLSKHSRAEVISIILYVLQEGIKDQTLLSCVYNQICNRANMTSSRESAESSQLQKGQNIQYPVACTVVSLATQSIDVIQYLLENETSMRFHFLADQEGSAFIKKTSKKNKLKDSSFKFPINILLNLLESKIIKEDTNLMDILSRSIQIATRPLPTMKLKLNELGKESEQLKKLPQLPHIPDRSLKQIINILVADECANKVFQQTIVSIQNLSVLDNARIVFPKELSKKATYLSSKIAKELRELINDLRNSKKDFENLPSLNQFSSGASDQAKLLRVLTALDYLYQAKDNDVDDIEELKLLYKNSALGPLWGALSDCLKLLREDDNMNYIAFILSPLIEALMVVCKHSKVQRMNTLDVLKYEDERELDFAKEPIESLFFTFTEEHKKILNHMIRNNPKLMSGPFSVLIRNPKVLEFDNKRVYFEQKLHGEFVNETREKLPINIRRNQVFLDSYRSIFFKPSDKIRKSILEIHFNGEEGVDAGGVTREWYQVLSRQMFDPNYALFIPVASDKTTFHPNRTSWVNPEHLSFFKFVGMIIGKAVYDGYVLDCHFSRAVFKRMLGKPVSLKDMESLDLDYYKSLVWMLENDITDIIVETFSVETDDYGEHKIIDLIPNGKDIAVTEENKQEYVKAIVEYRLLTSVKEQMDNFLEGFYSMIPQDLVSIFDEQELELLVSGLPDIDVDDWKNNTIYENYSPSSPQVQWFWRAVKSFDVEERAKLLQFATGTSKVPLNGFKELTGMNGISKFSIHRVYNATDRLPTAHTCFNQIDLPEYQNYTRLRNALLLALREGHEGFGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHG2</accession>
    <name>A0A1E3NHG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_35831</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the GSE complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016111.1">
        <property type="nucleotide sequence ID" value="XM_019162969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHG2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHG2"/>
    <dbReference type="GeneID" id="30179656"/>
    <dbReference type="OrthoDB" id="10020193at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000488">
        <property type="entry name" value="Small monomeric GTPase (Gtr1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="326" mass="37697" checksum="518B02F45935AA75" modified="2017-01-18" version="1">MGRSGSGKSSMRSIIFSNYSAFDTRRLGATIDVEHSNLRFLNNMTLNLWDCGGQDVFMDNFLNNTNENNYNNSSSANIFKKCEVLIHVFDVESKEVAKDINTFKRVLENLSKFSPNVKIFILIHKIDLVQSNKRQELFQLMYNNLKNIAYNEYGFKITGFATSIWDESLYKAWSSIVCSLIPNVEIYEQHLAKLNEIIDAREIILFEKTTFLVISSMDRFKSNTNEHTMNPTLALDPKRFEKISNIVKTYKQSVNKIRSNFKNLILHGKNSSIYLDVLTSNIYIMVIMDNNIGKDENYEVNNESLVLEDIKIARNLFEQIENKPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHH1</accession>
    <name>A0A1E3NHH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2415 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016708.1">
        <property type="nucleotide sequence ID" value="XM_019161388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHH1"/>
    <dbReference type="GeneID" id="30178075"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019417">
        <property type="entry name" value="DUF2415"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF9">
        <property type="entry name" value="DUF2415 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10313">
        <property type="entry name" value="DUF2415"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00210">
        <property type="entry name" value="HEMOCYANIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2415" evidence="2">
        <location>
            <begin position="320"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="702" mass="79539" checksum="D43E3008442FB9D5" modified="2017-01-18" version="1">MTIDLEVTQGKMSTARKERSLPDAHRFGQAVLKKRSHYLNVALTSPHWQLKDLLISSSYSSYDEDYGGNQDPTFYFPYKNRIFSFNYSALLGADGSARSNVHTRILKYSSIKFQSSPRCLKQLDGIVVMGGITDSFDPDTNPVGLQKGSFSVCNPQTAATENIQIGEFITNSVSINKLASSGSSHYRSYLCNNDKFLYQFDITPSRVIQSANPVYLKVALNHSILSNDNSTLVTVGDSPKIFISHPQEGTPKDGGGGSPSTKNFDILQTPGDCGFSTSFLSNGYQFITCFQDGLALIYDMRNLSAPLHSIHSTRPKTQPGAFRVVKTSNYNDDLICLSEHQGRVHLIDSRNFNNHSVLLLPKYLYNVPPSISVSYMNDNSCDDIHVNTDDDNSPSGMETDDDDARSSNHIGRERQDHYSNLFGKYPIAISTDSKKDLSKQWYNQPIVKDIDDFKDLSHFGGDLNLGYLESYRYMDTRFRNHGGTYSSNHNESRGEDRPYKIVLQGTFDREDLLKRKLSTNYSPQSSSLLTYKGSQVYPLQFRKRIGKFNRLKGDRNSDDMDMSDNENEDDDYDDEYDDDIGNKYTETRYISQQCWWNNENLGNAGNFREIWPWGKQVVSESGDDDSYNDDENEDSEVYDSSAIMETPLRDPFFYVDSDIEINGLELANRNGKSMLCIGTKEGIILWDINNWQRKCFPSFGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHJ8</accession>
    <name>A0A1E3NHJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73113</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016712.1">
        <property type="nucleotide sequence ID" value="XM_019164383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHJ8"/>
    <dbReference type="GeneID" id="30181070"/>
    <dbReference type="OrthoDB" id="1588579at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="300"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="443" mass="49630" checksum="28E7296F174CE413" modified="2017-01-18" version="1">MVYLVLWYGTSTLTAQLTKAILNQFDFPVFVGEFQFWFNFVLGYATIAASRRAPFTRIKLWFPQSTFPQNSKFVLSRQMFKLFFPMGTLQFVGKLFSLAATSICPIATVSSIRALSPLFIVLGYRLHYRILFSLRTYLSLVPLLLGVIIIVISQSNSSAESSDQISIYSEQLDNSSNNILYTAADSIGSTGSASTAHDLNTLIAHNPEQDISILSTIINTIHNNQIQLHGIFYALLSTSVFAAGSIYTKNVITNNNVGKSKEKKEIVRLALFSSDSTATLTALDLEKNEISNHQEEDYLDRVKEPKIDKLTTLMYCSLYGLAYSIPALLIYELPTLFSSKLYPATLGNTPAAQTTPIPYYTLIPWKLLIVNGFSYFFQSLLAFHILGMLPTVTYSIANMMKRIVVISISIIARGRHLTILEWLGLFHVTAGLYVYEKWGAKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHM1</accession>
    <name>A0A1E3NHM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016734.1">
        <property type="nucleotide sequence ID" value="XM_019164390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHM1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHM1"/>
    <dbReference type="GeneID" id="30181077"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="75187" checksum="54CB036DE1B07000" modified="2017-01-18" version="1">MKSPITAVPSVGNFLVVSNILRPGRNEICIQQLEQQDESNTQTQPAYNSNIEFQNDESVSNFKWIRQENEPASAAASAASTRKRRNSDSCSVPGNTERFTDYLVVLLATGEILIYSTFTKEFVNKISTETPFSAIEVINKLGHSKKALYNYDYDLIAFDATSSSLKYFSSNSPQLVNSVQFKHDNAISCILFNTSILDPKSTSNVELILASKSALYLLDQSHEVVKIIDITLDASNTASPTLASSPAKGKASKPKSKSKTANNNTSELVEPSSSLFSSSPVMKMMKNGTFLYIVRVDSPTIQIVDIDAKETNSFRSITASNNITDVSLLNINKSVLLSATLVDGSIELFHDKGESSFAKLEIEGGSDVGRFVGLLNSSNVIDGVYKGIWYDNFNVQITDFEFENISKLSGTITISVTELAEPTDEIIDLGEDEEDDTDDVKAKASSDDNANEAQQENVEALARNDDAEVTMEEKVKDYKCGELTQITELIKPRLVHKKHDDVYDKELAQILSQNTDYAKSVVYLLSAQDSTVMFSKIAFIISQYQHQPLNQGFDFVRSIKEWLKWLLVLRGSVLAEDDESVGWLRLLQSDFKQEARSLNGMIKLTGKLSLLKDQLTVRKEMMRRKADGDEEEEAVDTDAIDETTFAEDATSSVVLDGEGGFEDEEEEDNDNEEENNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHR9</accession>
    <name>A0A1E3NHR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016795.1">
        <property type="nucleotide sequence ID" value="XM_019164411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHR9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHR9"/>
    <dbReference type="GeneID" id="30181098"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013888">
        <property type="entry name" value="RNase_P_Rpm2_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08579">
        <property type="entry name" value="RPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="857" mass="98230" checksum="E3A910E4E4AC25F6" modified="2017-01-18" version="1">MVMRPFRNIAITKQKPGFSFSRNVSTNTYTHQFFNPSYISPKDLIKVDEVGFNSNTSSHEANRSIFKYVAPALSVTKNYVIFNASNSGNNEHVNSHGNRNNYLLQQEASAFRTLEESNDYDKILLRKLNHKGNKNGRLNISIDSVSQNHTVLNIRRANSNNIRKVAIPNKSLVASRNFSTDNLEKKFESLQIKDASEGVKDTETANEKSAAGNHVKFQQLNDQANRTFESIDDALDTFLYTDPVKSQEVIVDSMMSMGKFNEVLPVFIRMRNNEIIPSIEIYNKVLKSIQLRDTDETLETRLTHLLNTYSDMLSNNIKPNNATYELIIDNLVKGSIKSYQLSNYKNGFEFFKIAFELFLINHKESTSLSNTFKNNSIYIDLLTCLNYYKVKDVITPQNLFEILNKRIISQNQQHFHIQMIKFSTLFKDLHFIENLYNTEIKSVNSLPAKAYSYPNQDAVHQQLIESYNLCGDFKKSTLLLDTIVNNIPDKESSASQHLISEYLSIFLRSQSLVDPVLAFKSLYKFNNLKWLPAVSIESLVVLSYSFLRLNELQMALKVWDFALLRSDFDFQNKSLCSDKHSDEYSIYICNFHNQLVTAVLNTGDKNLILKSVREVLCKNSLVLDDALLVNLVRYLNMANVGNELVVKLTLNQGYKRIIRGSSAAPTSSERSLSNFLSLVVDFLPVESLLTVFNSTFFKRVVEEYRLVNDNIYGILKMFDTVQKQPSVLEESNVNDNTRLKLKYYAKVLDYEFDDADNCYVQIPPEIANFKQNIQQVTQYKAGKASLYTQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKVVLRLECVSCKTKLQLALKRCKHFELGGDKKQKGAALQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHW5</accession>
    <name>A0A1E3NHW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_26849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016799.1">
        <property type="nucleotide sequence ID" value="XM_019162466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHW5"/>
    <dbReference type="STRING" id="763406.A0A1E3NHW5"/>
    <dbReference type="GeneID" id="30179153"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="32044" checksum="A7C9096EDE02B927" modified="2017-01-18" version="1" fragment="single">MGKRKNGGGGGGKDKKRQRCHALIEPGQYGVYASCARFKEVAAAKEMRLLLQDAIEKHYPKAEEEGGEGEDAKEDEKEDEKEDEKAVDIEDEIAKELAELKKNDDRSKQNRNNKDLVLREIPLGVESLTFFKLKQPVTPSSLVVAVCRDLYASGAKTGRFVQKLIPVDRSCNATKIEFDKLLAATVDAFVQEETARGGDARTALETYNVNLVKRNFDTISRDEFMETVKEKLDDTFGAGWCRLQYKGARTLLNIYCFKNNIGMSVVPNDAFEELAKFNVQQIFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI15</accession>
    <name>A0A1E3NI15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translocon Sec61/SecY plug domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016321.1">
        <property type="nucleotide sequence ID" value="XM_019161701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI15"/>
    <dbReference type="STRING" id="763406.A0A1E3NI15"/>
    <dbReference type="GeneID" id="30178388"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071261">
        <property type="term" value="C:Ssh1 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="52803" checksum="84769EBAC6703EF8" modified="2017-01-18" version="1">MSGFRTLDFIKPLSSLIPEVELPYEKVVFDEKIVYTIGAAAIYLLLSLPLSGVNPNKIADPFYWVRLPFASEAGTLLEFGVLPIVTSAFLWQILSGFKFIKVNFTNIYDRQYFQSLQKVFAVLIAIVYASLLAISGYFQPIDAFAKTAATAGATFWANTLIVCQLGTATAIICLMVELLEKGYGFGPGVMAFIAANAATKLSGSLFGILSNPATLESNGVVIQLFRNIFNKPFTNAIYDVFARSGEANFIQVYLVIGVLGLVSYLHHFRMDVSIKSSKVRSMVSNYPIRLFYCGGLPILFSYSLLYNANILTFSLTKIFGGSPYLASWEIDPFSKKTYELTSGLLYFLSTSNSACCVYFNILRIVAFTLFVTITSATFGRYWYAMSGSSGKELAKQFKDQDIVVVGHRDATVGKELNKMISSASVIGATLLGAIIGFAESSGLAKGLVAGVVVGVLCSLSLLEQVMSEFQQSGAANSQFSQVFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI21</accession>
    <name>A0A1E3NI21_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">NAD(P)H-hydrate epimerase</fullName>
            <ecNumber evidence="3 10">5.1.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)HX epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_73282</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="10">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.6" evidence="3 10"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016892.1">
        <property type="nucleotide sequence ID" value="XM_019164434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI21"/>
    <dbReference type="STRING" id="763406.A0A1E3NI21"/>
    <dbReference type="GeneID" id="30181121"/>
    <dbReference type="OrthoDB" id="10064708at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032976">
        <property type="entry name" value="YJEFN_prot_NAXE-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232:SF10">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="11">
        <location>
            <begin position="12"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="62"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="145"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="256" mass="27573" checksum="B2AF8F79773A2D9D" modified="2017-01-18" version="1">MSSFKTLSAKSAAALDGSLMSTLGYKLEQLMELAGLSVAQAVYSDYKPAEYPNVVVLCGPGNNGGDGLVAARHLSLFGYKSVKVYWPVVGKNAFYGNLKTQLELFDIDIVQDASAGDSGASASASLPNLHSVLRDSSIIVDALFGFSFHPPLRKPFDSIILAMVEQQQKHGKKIFAVDVPSGWDVDEGPTARSGTEEYMPDALISLTAPKPCSLKLGPEVKHYLGGRFIPQCVADKWDFQVPHYQGADQYVKLASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI80</accession>
    <name>A0A1E3NI80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_19452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016952.1">
        <property type="nucleotide sequence ID" value="XM_019162119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI80"/>
    <dbReference type="STRING" id="763406.A0A1E3NI80"/>
    <dbReference type="GeneID" id="30178806"/>
    <dbReference type="OrthoDB" id="5069333at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="404" mass="46945" checksum="018891C298269013" modified="2017-01-18" version="1" fragment="single">EKSLLQYFIDAIGPTCICFPTPPSTNLSINTSTLTSSHSKANPYLHLIVPLAFQSQVVMDALIAASAHQLFILGNSKYEQLSDRYSERTIEQLSNTLRQKQRSQCTDWDDFLAAVLMLCFKEISSNRDYRSWVSYLNCAKFFLKKFNSQRTFSPLYKFFARYFIIHEVIGGTAWLQKKVDGDEFDHFAHDTVIDLVFGCCPYLITLIDKISNLGRCYEDLELEPINTRKEFEHYILIQRNELQSEIEGLDQRIQLFPEINSLSENCIKTIAEIKRLSTLLYLFARVDLETLYYNNGRATNYFIEKERCMNKVKSRLVSLFKNLPECPMSLLWPLFVLGLVATTDDDERWFVLDKLVYLQKARELGNVKTAKDVVLKIWKERDLGLISFRWKEMIKGKTESLSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI88</accession>
    <name>A0A1E3NI88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoglycerate mutase</fullName>
            <ecNumber evidence="9">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17099</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="9"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016947.1">
        <property type="nucleotide sequence ID" value="XM_019161463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI88"/>
    <dbReference type="STRING" id="763406.A0A1E3NI88"/>
    <dbReference type="GeneID" id="30178150"/>
    <dbReference type="OrthoDB" id="4818801at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000012">
        <property type="entry name" value="Phosphoglycerate mutase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010713">
        <property type="entry name" value="PRK14115.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010718">
        <property type="entry name" value="PRK14120.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="184"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="249" mass="27794" checksum="D24EF8232EDE39BE" modified="2017-01-18" version="1">MVTKLILVRHGQSDWNEKNLFTGWVDVKLSPKGEQEAARAGELLKEAGLKPDILYTSLLSRAIQTANIALEKADRLFIPVVRSWRLNERHYGALQGKDKAETLEKYGKEKFQEWRRSFDIPPPEIEDDSKFSQVGDERYKTVDPHVLPKTESLALVIDRLLPYWQDVIASDLLDGKTVLITAHGNSLRALVKHLDNISESEIAGLNIPTGIPLVYELDDNLKPVKPSYYLDPEAAAAGAAAVAAQGQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIM8</accession>
    <name>A0A1E3NIM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 8 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_17692</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016316.1">
        <property type="nucleotide sequence ID" value="XM_019161698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIM8"/>
    <dbReference type="GeneID" id="30178385"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000044">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="178"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="403" mass="44788" checksum="6EF1C49A3D240086" modified="2017-01-18" version="1">MSSTASPIAIAHTSGIKPFFSQKIQDYEIELAARTQNLRRLEAQRNSLNSKVRSLKDELKLLQEPGSYVGEVIKVMGKKKVLVKIHPEGKYVVNVTKDIDIKQITPSLRVCLKSDSYDLHKILPNKVDPLVSLMMVEKVPDSTYDMVGGLDKQIKEIKEVIELPVKHPELFESLGIAQPKGVILYGPPGTGKTLLARAVAHHTDCKFIRVSGSELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRSESSSGGGDSEVQRTMLELLNQLDGFESSKDIKIIMATNRLDILDPALLRPGRIDRKIEFPPPTLNARTDILKIHSRSMNLTRGIDLKKIAEKMGGCSGAEIKGVCTEAGMYALRERRIHVTQEDFELAVAKVMNKNEEGDVSVAKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIW6</accession>
    <name>A0A1E3NIW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017197.1">
        <property type="nucleotide sequence ID" value="XM_019161540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIW6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIW6"/>
    <dbReference type="GeneID" id="30178227"/>
    <dbReference type="OrthoDB" id="3976101at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="552" mass="63370" checksum="79122D574C3FCA32" modified="2017-01-18" version="1">MGINSLEQLSDPIVSRIISFLPQNDKVNLLYTNYHFYVLVQPLLYRNLLFTKSAALSCRHTYDESLYTVIGGLKTPLATASLNNKISKTRQSVLLESLTINSELCKYVENIVFAGSYSNDAKVESFHDVISLDLFEYVMKNCPNLKNTSAFDLSNYNKNFTKQQTVQLDSLKDLGSIINSPIKHLSIRSQDNLTDFNGLDDNLLLVFFGQLQTLEFNEDISQSILLDKLNSLSQNVSNDKEPLFHLQNLKVVFYHCFENYGEKLSSFLKRIDFKVLKRLEVAVGCDDMTCDCLQNFFHFLTKQDLKLQNLSFVQKTAHREHNYSEKFDFHITQFLKQYASRESLKFLSIRHAPPSDFNVGDGFEGNYLHRKALYEDVLPLLTNLETLICPTFLQSVAGYEQIISNLLWNGCECEHCNDYLPIFDKYILKHQYYDEEKSRMTDMISPILFGNVARVLSSRLPTGCDMFLDSFPPVCRYWDFHSAPYLITHSGECSIDKSASPPIAICVAHFLREYVDAIGLMIPTLRRCVLSGVFFDRIPVEGKDDNWVCSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ30</accession>
    <name>A0A1E3NJ30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_34275</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017189.1">
        <property type="nucleotide sequence ID" value="XM_019162873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ30"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ30"/>
    <dbReference type="GeneID" id="30179560"/>
    <dbReference type="OrthoDB" id="159299at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF0">
        <property type="entry name" value="SD19278P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="22693" checksum="485FD67ED502CAC2" modified="2017-01-18" version="1">MWLFGGTSKKEESAETAPARVAAEQEDTQLSMSALPVFDASKLHPLAGLDKDLEYLDLDEEKLNTVEGTGNGILPSRGWTDDLCYGTGTLYVSGLGLGGLVGLNEGLKHLPQGSVDPVTGAVKAAPFKLKLNTVLNQVTKHGPHVGNSAGVLGLLYNIIDSSLDAYRGRHDDLNSLASGALAGAIFKSTSGPKAMAYSTGLMTAAAAAWCGFKRMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJI3</accession>
    <name>A0A1E3NJI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_58623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017383.1">
        <property type="nucleotide sequence ID" value="XM_019163308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJI3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJI3"/>
    <dbReference type="GeneID" id="30179995"/>
    <dbReference type="OrthoDB" id="25503at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12885">
        <property type="entry name" value="SPRY_RanBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044736">
        <property type="entry name" value="Gid1/RanBPM/SPLA_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="140"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="625" mass="72140" checksum="1441B6A3E00FA07C" modified="2017-01-18" version="1">MTMHEIQQQPKDRLIPRYIQFQFLSNHIENELSNTSPDMLPDALRWRKISYLASLGYLGRYPGYFFDGSVRNEDYLNKVLNMNIISQEEDLYSTGDSHFAGNHSLLSSSKLGSIGGATEISNTDGSYYMEGNINWNSRNNLDDDWVMNHPLNVKDLSLPTRIDMPKTETKVNLSDDEKTVGTELIENTMFNLYPRGNNKSNFVTIPLDSYVSPLLCIYYYEITVIKGIEDECDIVIGYVKDEIKNIAYTSSSMIDLRGTDDRAVGWYGKNGIFTLWNDKRIEKSACRFGKGDVVGMGYNLYRDVFFITKNGLLVSELPSLGKFLDKSFEGRKNVRGFVPSVSLGSWCKVRINLGDAEDKQKGFRFDIESYVKKNKYNFVDKIKLSKIPPFSLPNKKVVKNDLDLTHYVDELVIGFLKYGGYLDTVKEMQEDLKALKRKEDPEDGYMAGLGKADNMLLELCHLKKMVKKYVMADELLKVRNLLETKYPGFFTTYRKINFRLKILKLIRMLISEKTDILKCVKFASQLKNLFKEEDCQYYIDQVSVLFSHENPRNCPEFHLYYDDNKTKIIHAIIMAINEQNRLPFISSLDLVILRTDQNLEAYVDKVQNGDKGPLLINMLEDYIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJI6</accession>
    <name>A0A1E3NJI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit J, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_58643</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017417.1">
        <property type="nucleotide sequence ID" value="XM_019163312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJI6"/>
    <dbReference type="STRING" id="763406.A0A1E3NJI6"/>
    <dbReference type="GeneID" id="30179999"/>
    <dbReference type="OrthoDB" id="5520611at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006995">
        <property type="entry name" value="ATP_synth_F0_jsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04911">
        <property type="entry name" value="ATP-synt_J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6953" checksum="21E585D987E6639D" modified="2017-01-18" version="1">MQFMGVKAYKFPLVKYYWPFFAGFGITTVLISKAASALADTEEYINDPRNPRFANGEVELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJS9</accession>
    <name>A0A1E3NJS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein YAE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein yae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_72448</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YAE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017484.1">
        <property type="nucleotide sequence ID" value="XM_019164201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJS9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJS9"/>
    <dbReference type="GeneID" id="30180888"/>
    <dbReference type="OrthoDB" id="20086at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038881">
        <property type="entry name" value="Yae1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829:SF0">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="9">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="170" mass="17817" checksum="CE4B30A50F03ED4F" modified="2017-01-18" version="1">MPSCSPDTCVCKKTGGTEVAAAAAAPGTSFAPGDDSDSDVWGSSSPDPSLEATEEVSRDVAALKRRHENRGYLDGLTKGGELGLQTGFDAGYPLGAQLGGLVGQLVAETVWRASNGQISVQTRDAALAELRIDKVLSSEHFDSELHIADPALHPTILKWSCFYDSLLVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJV3</accession>
    <name>A0A1E3NJV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL24-related N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72488</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017530.1">
        <property type="nucleotide sequence ID" value="XM_019164211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJV3"/>
    <dbReference type="GeneID" id="30180898"/>
    <dbReference type="OrthoDB" id="1727108at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL24-related N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="93"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="93"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="154" mass="17664" checksum="0DF6DEC178477D99" modified="2017-01-18" version="1">MKVEVDSFSGSKIYPGRGTLFIRGDSKIFRFQSSKSYSLFKQRKNPRRIAWTVLYRKHHKKGITEEVSKKRSRKAVKHQRAIVGASLDLIKERRSLKPEQRKAKRTEAIEAAKEKKKADKEKRKAEKAKLVASGAKISKQQAKGAFQKVHATSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK93</accession>
    <name>A0A1E3NK93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_130993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017619.1">
        <property type="nucleotide sequence ID" value="XM_019160014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK93"/>
    <dbReference type="STRING" id="763406.A0A1E3NK93"/>
    <dbReference type="GeneID" id="30176701"/>
    <dbReference type="OrthoDB" id="3998004at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35045" checksum="14E660CB6C09454F" modified="2017-01-18" version="1">MVLKMNGTKTTTDDREQWISGAGNFLRAAEGSQTMREKRVAAKNGKRPSLGQNPLFRALHSGGREARKVDQQRGRDEYDDRETQRGRAPRNELLQRLDGGSRFAKMTFSKGARLHTEAPPKQNALAAALGMGGRGGRKAGAAGQKGKKKVDGGGAVKPLPRGPRGAAPGGRTGSAGNGEVRGATFTGKKAHDITFRNASLLPFLRIENLTEDASESDITMVLTEQLGTVLKVLNMRTTHNKVPSVTVEAFFLHEDQLQSYMAALNGVTADGRVLKVSIGYHSTIINSDQLWNGVLEEVRLLRQQAMRRGQRPQKPQSLGVRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKM2</accession>
    <name>A0A1E3NKM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_73373</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016996.1">
        <property type="nucleotide sequence ID" value="XM_019164462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKM2"/>
    <dbReference type="STRING" id="763406.A0A1E3NKM2"/>
    <dbReference type="GeneID" id="30181149"/>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050334">
        <property type="entry name" value="Molybdenum_import_ModC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514:SF4">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="6"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="302"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="526" mass="58302" checksum="D8DF59EF006828EA" modified="2017-01-18" version="1">MSAALVRIVDAWFTRAGGLVTAGKSPRPVFSEAVSFTLHAKTRLAVVGGSAKTDLLGVLANQFVGHPVAARTFPASLRDAGFRTELLRFVNNGSWGHGAHDTSGGFTHLSSRYEFFKDLEVDEVVEEFIADQSYNSNTGFDRDRVHRLVQMLHLAGLQGRFITTLSNGQFRRARIAKELYKEPSVLCIDDPFLGLDPVATATVDDVLRTTASDPRLKTTLVLGLRVQDDIPEWIDDVVLVDEHGIQAQGKKADLLEDLARVKHEFHARHVRLQKQVQEKLAAAGATLRSRTHGGSSPGSTIVEMNNVNIRYKGVSTIKDLNWTVREGEKWHIRGRNGSGKTTLLSLITLDHPQSWNRSIKVFGVERAPGKVNYFDTNKYIGFTSPELHALYPKNHTVFETISTGYVVGSYIPPRENMTAQQAEKIDSFLEMLDLAYLRDVKFSAIPVSKQKLVLLLRAIINDPKILILDEALSAMTDEDVIKGKALVDQLNTTCLVIGHVDEEVPKCDKYIVVNGARHGVYEIGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKP7</accession>
    <name>A0A1E3NKP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_16557</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017835.1">
        <property type="nucleotide sequence ID" value="XM_019161254.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKP7"/>
    <dbReference type="GeneID" id="30177941"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000705">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1021"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="56"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="416"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="564"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="812"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="896"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1290"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="416"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="585"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="699"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="832"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="914"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="944"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="966"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="984"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1298"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1337"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1349"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="1050"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1376" mass="153086" checksum="873F282E1600B292" modified="2017-01-18" version="1">MSDSESVNLPRHRGTLNMDALATDRPSEEELYQPSKQIAPLKEIGASEIMEETISDFEDEEAVGNGHMNGNGKVQEDASASQDPVPDRASLHPHRSEITMITEELTQINNAKSGFDSSEEEEEKEEEEAEKRQLDKAGDQSNQQQGPTERAEQRHSQHGSHVQGDIEMQEDDEEGDRSEITTPEMKPVDNLDEHDLLLSTPRAITTQHNANRTPRSRHSAHNDAIAEVANFDFSTPDLLDFDDQLNQHQTLSPMIGEFEGMQLTNEENMQLLTPNRQLLYKNLEVPSESALMNERLDTHSNDLTEDKGSGSIFDINQLKMDLSSKDLKPPFATNMKTAASSDLDFNSIIENYNQIDNGITASTNSLDFVQESHRGGEKTQPSSAGAGTIANTHANVLNKSTSNTTVTQKADNLNSTLNERSSSTLPSDPTSFTSNSSRGHARSNSIPHDIKTRQLPKTSNSVQDLPNKQKKKKKLFNGFTKVFGMNSSSRSLSQDLKISAPKNVVLKTHVSYDSETQTYKDLPQEWARILTAQGISVAEQQANPVEAKEVLKFYSEAYGKGGMPGEGDKFMDVSSRNRTKRSSSDSDFMDSTQEVAQDSSNETTHITTDTSNSYPRQSYKSDTSLSSAANRNLYQTPRTENSNFSNTQTSPTFDSKGDIEYIPKRHAPPPPPSASTVSSSANQTRTPSSSGSKSISLSRHASLSNARKHLGSNNTSPVTNKRVISSGSTGRSAPNSPSTSIIESFSRRFSKKRSATNAADYKPRIIHLSEGITNPDGPIQISTPAQASTTAFISKGSLSPAILSNYGSPNVSDTHSLSYHEPKRAPPPPPSTFTSKSALDPSQGSTADEFLAPEPFSTLKSPVSLSKDIPPMSDVNDIIEEENDEVNEDMFKMNTTEKDIRTSHQVSKPEEDELQRPTEDELKAKIDKSLPPVPFPATTPATGNEDKRKSQEDSRLSSTKDKQSIPPIPIAPPVSAPAKSSKKQMSEEEQERRREIRRAKDVKYMRKLREICSQKDPSERYHDLVKIGQGASGGVYTAYDNVTNQCVAIKQMELEKQPKKELIINEILVMKGSKHGNIVNFIESYLLKKNLWVVMEYMEGGSLTDIVTHSIMTEGQTGAVCRETLKGLRFLHSKGIIHRDIKSDNILLSLTGDIKLTDFGFCAQIKDHASKRNTMVGTPYWMAPEIVKKKAYGPKVDLWSLGIMTIEMIEGEPPYLNETPLRALFLITTNGKPQLKERESLSSELQEFLDACLEVNPDKRANSVQLLNSKFIEMAANNLTLAPLVQLARTEKQKEQEQDQEQEQQDSEEDPDHEQEDYEEQEYVQEHVRSNVQEYVSDQEEEGEEEEQEKNQLHQHGHEPLQHDTERDTLEEFNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKV9</accession>
    <name>A0A1E3NKV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_72822</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017898.1">
        <property type="nucleotide sequence ID" value="XM_019164303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKV9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKV9"/>
    <dbReference type="GeneID" id="30180990"/>
    <dbReference type="OrthoDB" id="10249988at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000013">
        <property type="entry name" value="Coatomer subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF0">
        <property type="entry name" value="COATOMER SUBUNIT ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="10"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="190" mass="21576" checksum="95DB94B3768F8526" modified="2017-01-18" version="1">MSFNVSLYSIDAIIILDNQQNRIFTKYYKPPHETPENDLITNIKKQKQFEKDLFKKTLKQNTDIILFDNHTVVYKEYSDTIVYVIGGLSESEVLLYNVLQGLTGAFEIILSSQLDKRSMLESYDMLVLAIDETVDDGIILETDASAIAARVTNPPTEDAVNIKIDLSEKGLLNAFNFAKKNISERLQQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKY5</accession>
    <name>A0A1E3NKY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72817</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat PWP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017893.1">
        <property type="nucleotide sequence ID" value="XM_019164300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKY5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKY5"/>
    <dbReference type="GeneID" id="30180987"/>
    <dbReference type="OrthoDB" id="3142434at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000602">
        <property type="entry name" value="Periodic tryptophan protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027145">
        <property type="entry name" value="PWP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="142"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="329"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="457"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="7">
        <location>
            <begin position="736"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="870"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="873"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="912"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="924" mass="103747" checksum="F83B5029C41BA03C" modified="2017-01-18" version="1">MKSDFKFSNLLGTVYRQGNIVFSPDGTCLLSPVGNRVSVFDLIHNKSFTFNYEHRKNISAIALNPQGSLLMSIDEDGRAILVNFKARTVLHHFNFKNKVRSIEFSPDGTHFAVAAGRFVEVWKTPDLAEDRQFAPFVRYRVYAGHYNDVTAVTWSNDSRFILSASKDMTARIFSLHSEDKSVKMSLAGHRDYVVKAFFDSTQEVIYTVSKDGAIFRWEYTERPGEEELPEEEKTMSWRIISKNFFFSDASLKCCTFHSKSNLLIVGFGNGEFRLYEMPSFTLIQQLSMGSNGVSTITVNPSGEWIAFGSQSMGQLLVYEWQSESYILRQQGHFDSMNALAYSPDGSRIVTASEDGKIKIWDIVSGFCLATFDEHQSSVTGVAFAKRGQVMFSSSLDGTVRAWDLIRFRNFRTFTSTSRIQFSSVAVDPSGEIVAAGSLDNFDIQIWSVQTSQLLDQLSGHEGPISYLSFGSEANAKLASASWDKTVRVWDIFGRSQASEPFDLTSECLALSMRPDSKEFAVSTMDGKISFFDIESGRMIREIDGRKDILQGRYKEDEFASANSKRGKHFTTIAYSFDGLTLIAGGNNNSICMYDLPNEVLLKRFKISLNMQLNGTLEYLNSKNIVDGNAVDLVDRSGELSDAEENHKADLRLPGSHRGDAGLRSTRPEVRVMSVGFSPTANAFAAASTEGLLVYSVDDSLLFDPIDLDMDVTPENTLQCLNEGEYLNAIAMAFRLNEQYLIHKVYEGVNKDDIPLVAKDLPAIYVPRILEFIGSIAMESQHVEFNLLWIENLITAHGKFITKNKHLFVKGLRSIQRFLSRVVKDVIGYGYKVNYLVEFLLVSSKTENMVLEANHEIEDVIIEDIEVDAAGNHEDEEAEEESGDEDEEDNDEGWFGPGDEQKKYTDNFSNSDSDSESEEVIQDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKY7</accession>
    <name>A0A1E3NKY7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017341.1">
        <property type="nucleotide sequence ID" value="XM_019161102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKY7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKY7"/>
    <dbReference type="GeneID" id="30177789"/>
    <dbReference type="OrthoDB" id="441812at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="65610" checksum="CAF525397E5ED197" modified="2017-01-18" version="1">MATSVKTGELLEWFQDPANRCYFNSDLLTVKPSPISSGVGVFAKKTQAVIPTEDDVEENNLLLRVHKSMVLSPQTSTISNLLYDHGLSGMYGLVLAFIYEKEMGDKSPWWVYLKTINYNDDKGNYLLPLFLHSEKEKSILKGTEVDFMGGLDSDDLLEHFQTSCQFAEDVYDEVGIAVPNALKEENLNEFAAITLAVASRAFEIDNYIALGLVPGADLFNHDASGEENVHFVTLGEVCPYCGKEDGCGHEDYGPPESEEEEEEEEEEEKEEVSSERVEGDSEDDGSDAEVIEQLDEITMDYVEQIENELKEEKERKDAEDQEQSETSDDEDEEFDHSEFFMNPDECCDIVLERKIVKNKEVFNTYGELPNAVLLVKYGFAVEDNIYESLCVGPQIVNFRKENPHLEERFDWWSCMGWILLREEEKNNSKDEEEGDDSDEEEEEEEEEDEEEEEEESWLFQCRVDHPGKASTQLRAVAKLLTMSTVEFEAMVADESCDGEMIMRELANKKPTKEGKVLLKRWIQERYATVGDGKTRSGELKRQLRKTDPHSTRFCFITAVLNEKKLLENALHNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL16</accession>
    <name>A0A1E3NL16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_137091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017955.1">
        <property type="nucleotide sequence ID" value="XM_019160100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL16"/>
    <dbReference type="GeneID" id="30176787"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="253" mass="27314" checksum="2727C13E5A7DFC91" modified="2017-01-18" version="1">MQCQGGILRRGVDVARSAVCVQRGRRGVHGGCCGCSSFLQTGWQVSRYVCSVLSLCSLALCSRFVLYALWFFAFWLCLTERRTIVLRCCWVRRGPCGNKCCAGVSVERCSFCCPSLSRPPAPLLPPHAIFTAVSLSLSLSVRPSLPPSPPPLPLSSPPAGAGANPGEKRLWADRLKMLSDFCRCSAISADAQRFSVAPSGFRIRNGGCPFRGRARSSRRRGIKGEQFPGSDFSPLGSGDRSGANCLFHVYCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL81</accession>
    <name>A0A1E3NL81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72910</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017993.1">
        <property type="nucleotide sequence ID" value="XM_019164328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL81"/>
    <dbReference type="STRING" id="763406.A0A1E3NL81"/>
    <dbReference type="GeneID" id="30181015"/>
    <dbReference type="OrthoDB" id="68020at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046488">
        <property type="entry name" value="Sfc3/Tfc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007309">
        <property type="entry name" value="TFIIIC_Bblock-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04182">
        <property type="entry name" value="B-block_TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20222">
        <property type="entry name" value="DUF6581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="B-block binding subunit of TFIIIC" evidence="7">
        <location>
            <begin position="132"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor tau subunit sfc3/Tfc3 C-terminal" evidence="8">
        <location>
            <begin position="824"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="550"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="733"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1260" mass="143589" checksum="6FA0D727177511FF" modified="2017-01-18" version="1">MVDTPDTIVKKVLTELATNDFEGINAKEFWASVESACGKLDEFTKTVVWNWLIEKPDFEVWRKVEDGQDEGSGSGGKKSEKPSMTLEEKDQLPDYETFFDDVNINDYMIKVSEDFQSLYLTGVQQKDNILGKMPYDLLRIIAKHKEAGINSLDLIQESGQDKRSLTTRLGVLEDNLLITKFSISVKKCITNHMIHFRFAVKDTKTEDVAPEYYDRYQAMSQIIDALKKEENKLRLTKDLFEEIKESQPILKLRWFNKILKFLVDNGFIEMIQVEYTSRQRYFPGVRYLKDLPPANKKTKLLEKIREQSLNEKSQRDENDIGGLDDSEETIDQPNFNRYFPLTSQIHSLVLKNPGIMNAKFDSELTGTYHTRQISNIVEGISTNIPNPANVNAIVGQIFQIGKTKFYRFTTQNVLNRRDPNYKYVAPIEGEIAPASQTSLFEESVKYGSSYTIDRRFKIISVPVSGQEGQTKYYFISKSYSGALGSKLAAQVPTRFALQGSYESQNGWIKVDTKTKKVVKDIPSYKKVVESGNKNIAKFNKGIKDLQSQLKNSSGANENGNNRDEKADDISVNGASDVAGPEESVFNDKTAPKDAEQPSMDYGPEFRRARLRELTDENRCICINSEFCAKISRLMKVGYLIDRRTLIRDAISLAAKKLVEAEKLENGKFVVKSIKNPPTEELINECIVDVIKTSTRKFSAQVQFQNLPVRNEGTLIRGWKFADKEMRLQNAMKSVKSSSAGGNRRKRVKIINNGVEEQSEEDDDDGTENGNGIKSEAVDTGRGSVSNAKEDDVLEPLMDDRKRKKFKVANKSQARVVKTFKKIRTSVKVTNDHVLILIKAIVITQSLSVGGNIDWPTVAKVLDDIYGVEMLRRQWPRHRKMLGQRNLMQAKKNWEAALLDAVSNGIVTAADLENYDIFKMLDVWKSQGADIFINKTENEILSNYEDNFKTQVFKPLKDESGQEVFRESTSMIEKEHVWTSRNFMYPVDQTEHLELMNECENPSALQIAKTKLKALFATKVEKFSSNKVRDLFADIPKELYSKALTELENQKAIAFLGEDSKIKFTLTDRLMLTLDCKLDDDFANDAHKMCDILDEIDTSHNGILLSTRCPSGCYAPLLTLLSGNHIVVTRVDQEMDSPNTYYTKSLDRGKLESDFLISHYKKERVMLAKRVPVPLGAPCSLLWVDLEGDFNEKLWHKCVYVLIWSIVFHPGTPFDTLCARIDPLLEPFEVKRILDWMVMRGNVVTGQFGGYWPTECWYDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL82</accession>
    <name>A0A1E3NL82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TFIIE beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_27448</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017959.1">
        <property type="nucleotide sequence ID" value="XM_019162495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL82"/>
    <dbReference type="STRING" id="763406.A0A1E3NL82"/>
    <dbReference type="GeneID" id="30179182"/>
    <dbReference type="OrthoDB" id="3995672at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054600">
        <property type="entry name" value="TFA2_E-tether"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003166">
        <property type="entry name" value="TFIIE_bsu_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22254">
        <property type="entry name" value="TFA2_E-tether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02186">
        <property type="entry name" value="TFIIE_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51351">
        <property type="entry name" value="TFIIE_BETA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIE beta" evidence="8">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="205" mass="23832" checksum="7E61961394F5501E" modified="2017-01-18" version="1" fragment="single">LTMKLMASSDYIKSKDRDVPVFELETSLGFKVDPQLLKCLSNVDRIQYNPESSSFQYLSLHNIKTGEDLLRVLRNQPAYAGLSVKQLRDGWNNCLPTIARLEQENEIIVHKTKKDNSPRHIWLNFDNLPIRTLDMIFYEMWNAVNIPQHDDLVKLLLDNGLKPTNAEPESMKNKKITSVQERKQKKPRRGKITNVHMRGILKDYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLJ7</accession>
    <name>A0A1E3NLJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_61982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018122.1">
        <property type="nucleotide sequence ID" value="XM_019163465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLJ7"/>
    <dbReference type="GeneID" id="30180152"/>
    <dbReference type="OrthoDB" id="10293965at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133425" evidence="1">
        <location>
            <begin position="20"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="308" mass="34293" checksum="2EA63B44BE34EE0D" modified="2017-01-18" version="1">MLFLFKIILFYLTLITVAATPILNKKIFPFYDFLHTVAPIYLRAQFNGNPNVQYWTKIAWWASFVAGDGGLVDNVNVGIDACQSCIANHQSPNFNCRNDIVSLVKMSITNVTKGVVGWMSGYGANGVDGNGSPVKRQLSNRSGDADIDTFDPVIENWTSNNTTRHLATAHIRSGATMLSKSSINDEDGNTSIPFLSRQRLPNYATNYGNQTLVKRYPTLDYLCQGYMAVSYCADVNSIARLVDDNNDIESSVSEVYDLATEGSSWTSKFYKLYTTNSDDVSESWQASMRVFYIEPNSHPYETECDTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLN2</accession>
    <name>A0A1E3NLN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16168</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017366.1">
        <property type="nucleotide sequence ID" value="XM_019161113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLN2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLN2"/>
    <dbReference type="GeneID" id="30177800"/>
    <dbReference type="OrthoDB" id="2392202at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040540">
        <property type="entry name" value="RNase_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18497">
        <property type="entry name" value="RNase_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="6">
        <location>
            <begin position="231"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="645" mass="72973" checksum="53CE3A3BBBED5F09" modified="2017-01-18" version="1">MGKRSRLEDDSGPQEAESRISKRLKTEKREKKEKKDKKDKKEKKDKKQKKEKKEKKEKKETHEKADEVEEIEKDEQKEVKLTKNFAIHDLISVERSISLVQENLAKIMEITPTADELSQYVQHLVKSTNNGSSQDSGVTAKILLLSKSHKIQLAAQLKTLYQANKLRIFDQIINFSETTILNSADDVQLLLKERNRKAHEKGTSTEKRFNENSILPELPVLYDSVLEAKVFIHKSATNNDMHSSKYDQVQWNNERLEYLGDAVLETIVSDITEARYHDFDEGQLTVLRATLVKNETIEVLSRAYKFPERQQELLKFHVIKTDLNIDSRVGANKRIADLFEAYIGAIFIDKGRDGNAYDFIKAWLVDVYEPILNEFESIDQLKYLHLSSGLVSKLLGNDFNGKPSSPTIAEDTTVELKETKVAQQQEPVQKFPITITSTEPIDKLARGNLYALIGSAKLHPIFTPVRSYQSTGTHCVVNCTMDGELLGTGEGRNAKDASARAAMAALLNKELIEKYHLIRMMTPRTESVVKAPELEQKVIKKARASSLLQIPHFIPHADILTPDASAKANLMDLLSEQKVSAEIKCEPDQASQSILPMFKSTLKVNGKNVCDCIEASKKKGANKVAQWLLDEIEKHGKQAVLKELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLS2</accession>
    <name>A0A1E3NLS2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_57639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018180.1">
        <property type="nucleotide sequence ID" value="XM_019163250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLS2"/>
    <dbReference type="GeneID" id="30179937"/>
    <dbReference type="OrthoDB" id="3997526at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013334731" evidence="1">
        <location>
            <begin position="16"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="77" mass="7564" checksum="58CDC26C4C0B9A4C" modified="2017-01-18" version="1">MQFSIVSLFAASALAVSTQTSFSTEVDTITSCASTVTNCPAKNATGSTSIYTDGAAFVGGSYYAAGAAVLAAGALLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM96</accession>
    <name>A0A1E3NM96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inorganic phosphate transport PHO88</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018289.1">
        <property type="nucleotide sequence ID" value="XM_019160694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM96"/>
    <dbReference type="STRING" id="763406.A0A1E3NM96"/>
    <dbReference type="GeneID" id="30177381"/>
    <dbReference type="OrthoDB" id="18139at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20753" checksum="E10AC86A236C25F9" modified="2017-01-18" version="1">MNPAVTNLVLMLVAMQVSKKLDFEDPTTLFYVRLAFVVGQSLTLLIYLYVRFTIVKKNDLTTLKYLDAPNKMAGESESKLITTTVKDYDLKQIDSAIKGVFTGLAMTGFMHLYMKFANPLVMQSISPVKGALESNIVKIHLFNTPAVGDLKRPFKAAPSILSGLTGGDVKTDKSSIEKAEIAGTGGVKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMP9</accession>
    <name>A0A1E3NMP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sec20 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71477</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018518.1">
        <property type="nucleotide sequence ID" value="XM_019163935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMP9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMP9"/>
    <dbReference type="GeneID" id="30180622"/>
    <dbReference type="OrthoDB" id="46868at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005606">
        <property type="entry name" value="Sec20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056173">
        <property type="entry name" value="Sec20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825">
        <property type="entry name" value="BNIP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825:SF0">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SEC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03908">
        <property type="entry name" value="Sec20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Sec20 C-terminal" evidence="11">
        <location>
            <begin position="170"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="340" mass="38836" checksum="A4EECB13CE38978D" modified="2017-01-18" version="1">MSTQIQQLDSLNVSLRKAYAFVVQYVYTTDEGQQSLYDDTIGEIYTKIEIDLSLLESTIVPSLPPNKAKQQVQFSVYKLKERYRDVRAMYRWKKVDEKSKYLESQYLRSFDEAELRILNGDKRDDGNLTKATLSQDADPFISSAARRTNAYSSIDDDQLEELSAQERLLQQNNMLTDKLQNVNNLMKSTLLAGEINLNELEISTSTLSSLADSYAFFGSVLNKTNTLVKSINKASKSERQMIYRSLYFFISVCCWILWRRIFKRPVLLLLWLMMSPIKLLFWSTSYGKSQPLNATSIFSSTLLTLAATISTTIPDGTTSITTASTIAFTKIEEYLSKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMQ8</accession>
    <name>A0A1E3NMQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_15366</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018528.1">
        <property type="nucleotide sequence ID" value="XM_019160794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NMQ8"/>
    <dbReference type="GeneID" id="30177481"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141164">
        <property type="term" value="P:mitochondrial protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000002">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000175">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048438">
        <property type="entry name" value="Yme1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21232">
        <property type="entry name" value="Yme1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="257"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="672"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ47415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="693" mass="75833" checksum="63A11FD192ACEE01" modified="2017-01-18" version="1">MLKNGSFLLSSSHMLRPTRCSRAVQAALLHKRLSLVKRFASTVPSTQDLAALEKEANNEIANPSIQAAFYRSLLDASYPHIVVSRYETPGISTDNDCAKLYLEALLKMGNKEKAETLSKSLNVPLPALDPSAVSPNATSGNSGNNNNNNNNTNHSGSKQDPIHVVVTETFWMSLSRWIKWLVPLGLLSYAAYYGFNVLVENGSIVKGASADDKSIEVTDSDVRFSDCKGVDEAKAELEEVVDFLKDPSKYVSLGAELPKGVLLTGPPGTGKTLLARATAGEAGVPFFFMSGSEFDELYVGVGAKRVRELFSKARARAPAIVFIDELDAIGGKRKSRDQAYAKQTLNQLLVELDGFSQSEGIIILGATNFPDSLDKALTRPGRFDKIVPVDLPDVRGRIEILKQHLKKVSVSKDVDPSVIARSTTGLSGAALKNLVNQAALYAAQNNALSVNMSDLEWAKDKVLMGGERKTMVMTEETRRNTAYHEAGHAIAAMFTNGATPLYKATILPRGRALGVTFQLPEMDKHDITKQECFARLDVCMGGKIAEEMLYGPNNVTSGCSSDLSSATGMARAMVTSYGMSDKVGPVKLSDKWETWSPKLRDLADQETRDYLIEAEKRCRELLNDRRIELKRLAEGLLEYETLSKDEIEKVVRGEKLDKLKVMTNKVIKGPNTRKEFDVAPEDPQPLGSIGKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMV9</accession>
    <name>A0A1E3NMV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <ecNumber evidence="3">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71533</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018574.1">
        <property type="nucleotide sequence ID" value="XM_019163945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMV9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMV9"/>
    <dbReference type="GeneID" id="30180632"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="9">
        <location>
            <begin position="71"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="252" mass="27033" checksum="58B311D0B78E52A4" modified="2017-01-18" version="1">MVQKIVKVCGIKSEEAANTAIVSGASLLGTILVPNRARTVEADTARRISKLCYEKRVEKKSRFVSSKDLLQHARTLEATGPQWFEEVCETITENGPYLVGVFRNQPLEDVIRISDELGVDVVQLHGSEDVDAFVAALELPVIPRFVLSKPGIENALSTHKFLLPLFDSEQGGEGKLVDWRDAARFGGDLNGRYILAGGLTPENVGAALAVGGCYGVDVSGGVETEGHKDMAKIEAFVGNARKAEAVKAGQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN44</accession>
    <name>A0A1E3NN44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="8">ATase</shortName>
            <ecNumber evidence="3 8">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_72876</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 8"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017956.1">
        <property type="nucleotide sequence ID" value="XM_019164316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN44"/>
    <dbReference type="STRING" id="763406.A0A1E3NN44"/>
    <dbReference type="GeneID" id="30181003"/>
    <dbReference type="OrthoDB" id="191723at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="556" mass="62058" checksum="9C507873417C64FF" modified="2017-01-18" version="1">MCGILGIISSDTNTNVASELFDGSLYLQHRGQDAAGIVTCGRGGRLYQCKGNGMARDVFTQERMSKLVGNMGIAHLRYPTAGSSANSEAQPFYVNAPYGISLSHNGNLTNGEELKKYMDEVVHRHINTDSDSELLLNLFAAELATYDKSRVNENDIFKALEGVYNRVRGAYACIAMLSGYGIIGFRDPHGIRPLLIGERLKDDGQRDYMLASESVVLKAHGYQVYRDILPGEAVIISKDKMLPVFKQVVPLKSYSPDIFEYVYFARPDSVLDGISVYRSRLLMGEKLAQNITDYYKSQDKNVLNEIDVVIPVPDTSRHSALQCAITLNLPYREGFIKNRYVGRTFIMPDQKQRQSSVRRKLNAMASEFYGKSVLLVDDSIVRGTTSQEIVNMAREAGAKRVFFASCSPVIRYNHIYGIDLADNKQLVGYNRTENEVSLKIGADHVFYQKLEDLIDCCQRDDSLKDHERELALTPVVSRDNSIISMIARNDEAPEITGFEAGVFTGKYVTGDETSYMLRAEQARARNARRSDSGSTGMDILDVKAPSEINIFNSGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNC9</accession>
    <name>A0A1E3NNC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RRM Nup35-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15532</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018713.1">
        <property type="nucleotide sequence ID" value="XM_019160865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNC9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNC9"/>
    <dbReference type="GeneID" id="30177552"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007846">
        <property type="entry name" value="RRM_NUP35_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527:SF6">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05172">
        <property type="entry name" value="RRM_Nup35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51472">
        <property type="entry name" value="RRM_NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6 8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM Nup35-type" evidence="10">
        <location>
            <begin position="249"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="59"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="466" mass="52180" checksum="03A162E3075E18E8" modified="2017-01-18" version="1">MQSSLFGGANPFQQQPPQQQVPSVFNNVNPQVQQMTQPSRFEKYELKNAADNRLFASESLSHSNQSGNSASGNGSGNSGGNGSNSLIKKRTIPNRLIKRVGKMDSSLGEEEKTSGPVLPFKVSTQVQPDTFAEDFAHLYKVDRPPSNSMFDPTVFQDETTPGEMLIGESIFKEMSENPDLFENVFKRPSRKLLQSETHSKYQNTEYPWSNSTLQSYNSEELPNTSTRKKQPNLHYSNALDISRSQQSAEKLYCSVIIYGFDDDHFSLIVEHFAKYGKIMENLVISDYTYYYGAFGNVIAHDKLVDVDEKEKSSLDSKKKGLELFPLFLGPQWVKVTYDNPNSAIRALADNLTDDGKGNVLGVLPYRRQDLEILLSHEIPDELNIGEGLHGLSHELELEKRLADNEIGRYLSNARGDTLDSLNGTKRNASSLTLKDGTKLLLKTKKESDGKNIWHKGMGFLFGEGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNI5</accession>
    <name>A0A1E3NNI5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_30998</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018775.1">
        <property type="nucleotide sequence ID" value="XM_019162667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNI5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNI5"/>
    <dbReference type="GeneID" id="30179354"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF29">
        <property type="entry name" value="IRON TRANSPORTER SMF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="476" mass="52522" checksum="C7297E0C7C2BFC07" modified="2017-01-18" version="1">MCLDLKKLIKFVGPGLLVSCAYIDPGNYSTSTAAGAQFHYAHLFIILLSNVFAVILQCLCIKLGTVTGLDLAENCRKHLPHWLNITNYVLAEIAVVFTDLAEVVGSAIAFKILFDIPLQIGVVLTILDVLVIIFAYNPDGSMRHIRKFEIFVSTLVIMTFGCFVLLLSKVDINSKREIWEGFLPSKILFKEKNAIYLGLGIIGATVMPHSLYLGSNLVKPRLREFDMKYGSYNSEIDDRTNTVHKPSLNAINYCLNYSYIELILSLCTVAIFINSSILIVSAAALFGNPGADDADLLSIYDLLCHYLSNQAGLIFAFAMFFSAIAAGVICTMSGMIIAEGSVKWTLNPFLRRLVTRTISIIPCLFMALFSGRSGIANILNLSQVVLSLILPFVTAPLLYFTASKKIMSVKVTNSQENEHTQESENSALISHSGSLGIVDFSNSLSTNILGFVAWFTIGFLNVYLIIQWLRGEDIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNJ4</accession>
    <name>A0A1E3NNJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018785.1">
        <property type="nucleotide sequence ID" value="XM_019163990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNJ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNJ4"/>
    <dbReference type="GeneID" id="30180677"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="302"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="458" mass="50631" checksum="F13188B848279FAA" modified="2017-01-18" version="1">MSFLSTLFCCFDAEDSAVKENNITTTHPKLTKTQSNLQRALTNSKPNSNSSLNLNKSHSPPPTNHAGASTANKSIHPSSASKKGQPDKSSSNTSGTATSHTNTGSSNPDRKKDLANGTKHSGATKGNSAASKHSSTGTSAAHPDNSQAPNKNMDAHNRNKIAHKMSYSDSNSTTHHVSNDDKEMVEEITDLKDSTDSSKDEDDAYQIHEHQTQNTDETDLNHQPESKNKVSNDAIAEDRGSPKSPPNSDSNPISNLNPDSTVNELNYEEQLYDEQFGTSLTAILPDQVVSSSGWLLDSQPKQFKGRKCLVLDLDETLVHSSFKYVRQCDFVIPVEIEDQIHNLYEVVVFTASVSRYGDPLLDILDTHKSVHHRLFRESCYNYQGNYIKNLSQMGRPLEDLIIIDNSPASYVFHPQHAVPISSWFSDSHDCELTDLLPFLEDLAKREVDDIRLVLDINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP71</accession>
    <name>A0A1E3NP71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_31448</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019004.1">
        <property type="nucleotide sequence ID" value="XM_019162701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP71"/>
    <dbReference type="STRING" id="763406.A0A1E3NP71"/>
    <dbReference type="GeneID" id="30179388"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF178">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02040)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="58430" checksum="523DE0BD06477BCD" modified="2017-01-18" version="1">MEREEFVNGVPGTELLVDIKKDNENVIEGAEIVYIPPPTECGADPLGWARYKKYWQLFVVSLYACCFAYGENNLGAAWTTVSEDIGVNMNNMNGGSALNWLLLGFFNVLWIPTAMKIGRKPVFIATTVIASCAMVWIGKCNGNAQWFLAMTVNGLGVAGYEAMIQLTIFDTFFVHERGRALAVYLSSQQLGSVIGLVSGGAQADTIGWRWSQYIVAIADAVVFVLFVFTFQETLFPRFLFERGEHSKFLEQMKSVDLTDGVSEYEIPNAQDFPAPSWGKIVTKWWVYYPQDKTTFWQYFRRPFILFTFPNILYAGFNFGFGGTAGMLSFSTIAEILMEDPYNYSTSTTGLMCLGSLVGSGLGGLAGSLSDYIVIFFAKRNNGVKEPEMRLFAMIFPFCFGAIGYMMYGWGAEQGDAWPVISVGIGFMTAQQVSSCSIATSYAMDCFRGISGELVVVLAIFSACINFAVSYSCQEFLTAVGYGWLMFFWAMLVLSANASAVLVMFQGKSWRKRSADKYFKFVAEGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP73</accession>
    <name>A0A1E3NP73_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15755</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018990.1">
        <property type="nucleotide sequence ID" value="XM_019160948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP73"/>
    <dbReference type="GeneID" id="30177635"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133450" evidence="4">
        <location>
            <begin position="18"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="85" mass="8215" checksum="74EFAFC0E722360A" modified="2017-01-18" version="1">MQFSVIASLALASLAAADTVLTQIGDGQIQAPTTQNQQTTATATTPAETSTSTTASSGVPQFSNAAPKAVIGGGVVALAGLAALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPC9</accession>
    <name>A0A1E3NPC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71925</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019029.1">
        <property type="nucleotide sequence ID" value="XM_019164049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPC9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPC9"/>
    <dbReference type="GeneID" id="30180736"/>
    <dbReference type="OrthoDB" id="425602at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120136">
        <property type="term" value="F:dUMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000679">
        <property type="entry name" value="Thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="10">
        <location>
            <begin position="8"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="207" mass="23544" checksum="67AA65657BC782BE" modified="2017-01-18" version="1">MRGALIAIEGLDRTGKTTQTERLLAKLKELNITHKLVKFPKRDTQIGKLINTYLTEKSFDLNDQAAHLLFSANRWEVIEEIKKDLQDGTVVILDRYVYSGVAYSAAKGLDFQWCLSPDIGLPKPDLTIFLKFRQSENASRAGFGDERYEVAEFQEKVKHIFENFSTNSEWSPLYVDGLSIDQVHGKIWALLQKYLGGLDNGLQVFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPG2</accession>
    <name>A0A1E3NPG2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_121621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019058.1">
        <property type="nucleotide sequence ID" value="XM_019159892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPG2"/>
    <dbReference type="GeneID" id="30176579"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="65" mass="7199" checksum="49591DACD149A0BD" modified="2017-01-18" version="1">MKAKFVRNYPSGPLLSNSFSSYFSIHRCLPLESTISVDVFSNRSVPSVKRYQGRTAAVLETGSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPG3</accession>
    <name>A0A1E3NPG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_122094</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019092.1">
        <property type="nucleotide sequence ID" value="XM_019159898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPG3"/>
    <dbReference type="GeneID" id="30176585"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="134"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="227"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="594"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="274"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="830"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1010"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1103"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="845"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1020"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1031"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1052"/>
            <end position="1073"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1124" mass="128283" checksum="CDB127CFE8BD73C1" modified="2017-01-18" version="1">MMSSNLQDSFSTENLELKSEISKNLNDDDDENSSKNQSVTGSSPFYAIKILSLPPTTQLDSLTLKFLKEKNLNSDDLQLIKRTSDNEITHNFIEFFHNKQLLDDFIEFFKIEESVDLDYELIDNEIQLNYQLPGHLFIRGLLYTTTAQDLYNIFKPYGEIHACKVIFNDYGISKGFGFINFSNKVEADNAIESLNGCNVDGNNLFINHHVSKKDRLKELEYKMNKFSNIYIKNLPADVTKVELSDLFGKFGKINSIFLPKDLNHDVDIQSNIDHHEHNSKDVINDDVQNDGSNDRESEDNPSSKAYGFINFKYHEDAINARKEMNGFEIRPGYNIQIDRAERKKERIQYESLAHHFNINTNKICSSDNDSTSSATITRNIDNSGEFHRMNKDVYEEPRIEKFQSPIDRSFSFSSNSSSRSRRQSSITSPQILPSFAMVNGNFNYDTTSNHNYHNFVHNQSLKNSNQRLQYPQMYQPIIPFTYNHKNKSQELLLQPEKQLQKAYQQQQQQQVQQPVQSQPQHIQESPYFANNKNNVYDRKIDHSISMIKGIPMNNYQPSITNQNPVLPPFIITPDSNLISTSTGLPIAGPQFQDSNLYVIHLPKEYSDEDLYDLFEKYGKITSSRVITYQPEEVEQLNKKKKYDRAKQVDSTAAIPDAKLGVPEDFAAAQLENNNSASTENQELNSDADRIPLVGESKCFGFVCFQNPVDASKALVCMNGFKVDSNHVLRVTFAQRKENKYNQGRLHHYNQNHLGGLYQYVNNYNNSYAHIMTPSGNIPPMPASSIPSLSPMGAMPAMGPIGPVAHLGPMAHMAQMAQMAQMAHMAPIPPSQLPPSTSPSLPTVAMQHSPSIPSMNGVSNVQSSPAMEMMHGVSLIGSSQHQPQSHYYQQQPPQYLPQNSPQQAAIPAMNSIRLSGITMMNNTPGVNKYNNWYYPSSYHQGGSSRERYDEFQNRQMLDVENGMQDLTIGLSDEDDYENYEEYEDYENYEHTNEDGDMNNDDDAQVESVEEITHKNASISRSRIDCNNRRNDANNNFSSTSNNSKARSKYTRANSYNSHSRRNYNSGSNNSSSVDINQNGYYNYNYYDHNYGSYVYNRGNYKQHNRSHSMINSGGFGSQKRQERRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPG8</accession>
    <name>A0A1E3NPG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15823</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019068.1">
        <property type="nucleotide sequence ID" value="XM_019160980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPG8"/>
    <dbReference type="STRING" id="763406.A0A1E3NPG8"/>
    <dbReference type="GeneID" id="30177667"/>
    <dbReference type="OrthoDB" id="10249672at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000001">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="11">
        <location>
            <begin position="50"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="11">
        <location>
            <begin position="209"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="12">
        <location>
            <begin position="815"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="13">
        <location>
            <begin position="965"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1103" mass="125921" checksum="F7239BB1C0D56268" modified="2017-01-18" version="1">MSTAEGPKAVVMENTARRDTLVAIEKKYQEIWDSEKPFEIDAPTNEEVAYGTSWEELHEEYPKFFASMAYPYMNGVLHAGHVFTCSKVEFAIGFERLRNKKALFALGFHCSGMPIRAAADKLQRETELFGDDFSGYAKESHETAEVTETETNEASKTDPTKFTAKKSKVAQKAGNAKYQFEIMQQSGIPLDEIAKFKDPYFWLTYFPPLVEKHVKSFGGRVDWRRSMITTDMNPYYDAFIRWQMNRLRELGKIKFGKRYTIYSVKDGQPCMDHDRQSGEAVLPQEYTGIKIEVTEFTDAAADYFKKENFDLTGKKVFLVAATLRPETMYGQTCCFVSPKINYGVFKASDNEYYITTVRAFKNMAYQDLTPVRGDYTPSVTVNGSLLVGSKIHAPLAVHESLRVLPMETILENKGTGVVTCVPSDSPDDYVTTRDLFNKPEYYKIEKDWVIEEPVPIVNTQKYGDKCAETLVKELKINSPKDTNQLAAAKELAYKEGFYNGTLIIGKYKGEKVEVAKPKVKQDLIASGDAFVYNEPESAVISRSGDSCIVSLEDQWYLDYGEENWKEQALECLGQMNTFHSETRNAFEGVFNWLKNWALSRTYGLGTKIPWDEKYLVESLSDSTLYQMFYTINHLLHSDFYGKKVGPLGIKAEDLTDETFDYIFCRGDSLENINIPIEKLNLLRREFEYFYPMDQSISGKDLIPNHLTFYIYSHVALLKKTFWPAGIRSNGHLMLNNAKMSKSTGNFRTLEQLVEKFGSDASRIALADAGDTVEDANFDESNANAAILRLFNLKEWFEEMVKSKDELRTGPYNFFDKAFDNEMNQLIEETYKNYDITYYKAALKTGLFDYQYSRDFYRELSLSSGAGLHKSLVFKYIETQSLLLYPIAPHFTEYVYRELLGKKTSINNAKWPVPEAAVSNELAAALTYTRNLQRIIRESEGMALKKKKGGKSDVDKDQDCLLTIYVCNSFPEWQQKYVDIAKKLYEEGKLDDMKIVKEKIDGKDMKRAMPFINELKKQLGVEKPEEVFNRELLFDELDVVKQAIESIKKAPASISVSKVQIIFFSNGSTTGKDIVSEEEISIPQKVKSIENAVPGQPAILIKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPL0</accession>
    <name>A0A1E3NPL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PAN2-PAN3 deadenylation complex subunit PAN3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Poly(A)-nuclease deadenylation complex subunit 3</fullName>
            <shortName evidence="7">PAN deadenylation complex subunit 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PAN3</name>
        <name evidence="11" type="ORF">PICMEDRAFT_51023</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and with PAB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein PAB1 (via PABC domain), conferring substrate specificity of the enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the PAN2 nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of PAN2 in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal zinc finger binds to poly(A) RNA.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for PAN2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. PAN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019069.1">
        <property type="nucleotide sequence ID" value="XM_019163150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPL0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPL0"/>
    <dbReference type="GeneID" id="30179837"/>
    <dbReference type="OrthoDB" id="204958at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3700:FF:000001">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex subunit PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03181">
        <property type="entry name" value="PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030844">
        <property type="entry name" value="PAN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041332">
        <property type="entry name" value="Pan3_CK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272">
        <property type="entry name" value="DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272:SF11">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18101">
        <property type="entry name" value="Pan3_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25586">
        <property type="entry name" value="zf-CCCH_PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="56"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Knob domain" evidence="7">
        <location>
            <begin position="525"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="486"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="326"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="382"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="618" mass="69465" checksum="DE0C088A85316583" modified="2017-01-18" version="1">MSGSQYDWAKNIPCRNIQIHGFCKFEHKGCSYNHDPALQKSSAVAPVGATANTVSAGESTITSAGTNSTPSILSQKAGYPLPTNDDNDSANNSTTSVVNGTNSPVLNKKFNLDSPAFRPTPLSTKFATLSPSLDHIPTFVPSTSNDTITSMNTNFSPHPQPPTGEDLFYQNTNLYPVNFLLYAPPPPPHVELNKKPNERTVDDLFINNKLREYLQLKNEESLKSISSNELDLPSHVGQYHSLYPIDHNFDYSTKSFGYVSIVYKCMSNENGKLYTMRRLQNVPITSPSVLKSIKKWKKVDCSNCVKVYDVFTTRAFNDNSLILIYDYYPMSLTLLENHFMSFPGKNPDLITEDSLWNYTIQLLNAITEVNRLGLSLGSLDSSKIIITNKGRIRLSAVGVNSILEDVNKTLPKDAKEIEEPEKDSDLLNLGKLILFLAKSTTYLKAPTEDPMEIIMQLKFTNVFKKMLSYLFREDATIEKFQELVAPMILKLANGLQNSCDYMESNLMGELENARLVRLFAKLDFISERPEVVKDGLWSETGERYPIKLFKDYVFHQIDDNGKPVVDLTHVINCLNKLDAGVDENILLVSSDEMTCLIMSYKELKELVNKSFLKLLGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPR2</accession>
    <name>A0A1E3NPR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_58401</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019202.1">
        <property type="nucleotide sequence ID" value="XM_019163297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPR2"/>
    <dbReference type="GeneID" id="30179984"/>
    <dbReference type="OrthoDB" id="996720at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002673">
        <property type="entry name" value="Ribosomal_eL29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01779">
        <property type="entry name" value="Ribosomal_L29e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="63" mass="7081" checksum="7DE8C35FF57F0A48" modified="2017-01-18" version="1">MSKSKNHTAHNQTKKAHKNGIKKPKTHRYPSLKGVDAKFRRNHRWALHGTAKALAEKKAAAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPY5</accession>
    <name>A0A1E3NPY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_32529</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019238.1">
        <property type="nucleotide sequence ID" value="XM_019162775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPY5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPY5"/>
    <dbReference type="GeneID" id="30179462"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF45">
        <property type="entry name" value="RE50040P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="772"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="13"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="12">
        <location>
            <begin position="442"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="709"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="709"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="810" mass="92214" checksum="4472FEEB2347C427" modified="2017-01-18" version="1">MKDLSNIVLNNKYQLKKRIGSGSYGIVYSAVHLYTGISYAIKIILKNNSSNTANNKQLLVELEKSLIYQSLLNNGCLDANLLNLDLIEKYGTDCKFLREISLQLKVHKHPNILSIYKVYDYQNSIFVVMDYYPDGDLFTTIVDKQRYKDDPFLIKSVFIQLIDAINYCHSKSVFHCDLKPENVLVTENGTRLILADFGLALQEKFINSNISCGSSYYMSPERIQNFSQAFNSLDSHSIHLERLLNPSSSNNNYPVHGNVKFPTSAGDVWSLAIILINLVSIRNPWLKASLHDTTFKAFVQNPRILMKILPISIEVFNILCKYLALNPWERGTLFEFREDILKCSKLTETGPLSIDSTIKSMDIITTESQMNIINNYPSNLSQFEVCPIPVDHVKLDKIRSGEMEDIDHVEVENDYMPKIPKECKSCVNCGFGYEASDPCSSVNSVALSYLSLPPYLDSNNKIPHYKNGGNMLLARNSDYVRLVKDSPKNSGHLFSKIPISEDDLSAFEVELEFKITGNQEKISLIGDGMAIWLTAEQLNQGDVFGMQSNFNGLGIFIDTYKNYNSKRNRHAFPYLSIQRNWEDPFHYDKATDGTGTEVGGCSLHRIYNNEESTKLKITYVRDANVFEIDVDVEGNGDWRTCFRKENVKLDELVPAGKPVYLGVSAETGDLHHNVDLYTINANTFRNKDGSLITEIDSLGEGIRVIDTESSKKEADDRSDSKGSGNVDREVRRRSRRTMSRLRRQEKKLKELDRIKYGSEHGFVGWFFNWVKYIFKVIFMLILVAIALYACVIGYRVHREKQRKKAPSGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPZ5</accession>
    <name>A0A1E3NPZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Aurora kinase</fullName>
            <ecNumber evidence="2 19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_32358</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2 19"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018901.1">
        <property type="nucleotide sequence ID" value="XM_019162762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPZ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPZ5"/>
    <dbReference type="GeneID" id="30179449"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005828">
        <property type="term" value="C:kinetochore microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044779">
        <property type="term" value="P:meiotic spindle checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044774">
        <property type="term" value="P:mitotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901925">
        <property type="term" value="P:negative regulation of protein import into nucleus during spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901673">
        <property type="term" value="P:regulation of mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="8" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="24"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="151"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="16">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ47788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="293" mass="34135" checksum="1F0AC1FEB789349B" modified="2017-01-18" version="1">MPRRSTVLPSQGKGQTRQWKLTDFEIGKRLGKGKFGKVYCVREKSSGYVCALKVMSKQEIVSYHIEKQIIREIEIQANILHENCLALYGWFQDDQNVYLVVEYAAHGELYKTLTRLKRLADPLASYYIYQVARALKHLHSKHLIHRDLKPENILIHLDHRVKLADFGWSAYVGGDPAPNTGNRRTTMCGTLDYLPPEMVEARQHDQRVDVWALGVLLYELLVGHPPFEEKHAGDTYKRIARVDFSVPEWVDADARDLLAGLLRYDAERRTTLDDVVMHRWVLKNKLRWEKSWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ24</accession>
    <name>A0A1E3NQ24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_58195</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018931.1">
        <property type="nucleotide sequence ID" value="XM_019163285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ24"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ24"/>
    <dbReference type="GeneID" id="30179972"/>
    <dbReference type="OrthoDB" id="409889at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21157">
        <property type="entry name" value="PUA_G5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000008">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000018">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF3">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="326"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="442" mass="49592" checksum="7E5378F9DCB15B90" modified="2017-01-18" version="1">MAAEELHETEDSRKFTIVVKLGSSSIVDEKTKEPKIANITQVVESLVKLRRQGHKVVLVSSGAIAMGLNETRLDKKPKRLAAKQALAALGQGKLISLWDNMFRYFQQQTAQILLTRNDIVDFSQFKNAENTLSELLNMGIIPIVNENDTISTQEIKFGDNDTLSAITAGMVNADYLFLLTDVSCLYTDNPRVNPEAKKVLIVKNLSNLNVNTKSGAGSSVGTGGMETKLIAADLATNAGITTIICHSNHPENILDIVHYSHEFDNSYRYSELESEETEEEFSVSNLKEEQRLQELNMPLHTRFIGRPKNHVKNKQFWLLHGLKPKGSLIVDYGCFLAITRQNRAGLLPVGIIEVEGNFNQLECIELKLGFKGSDKTVAFGRGRCNYSSGEIDKIKGLQSDKVSERLEYCDSEYVAHRENLAFPIHWDNELLEFLKKNTERKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQL6</accession>
    <name>A0A1E3NQL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein kinase ATG1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Autophagy-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Serine/threonine-protein kinase atg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_56636</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019455.1">
        <property type="nucleotide sequence ID" value="XM_019163191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQL6"/>
    <dbReference type="STRING" id="763406.A0A1E3NQL6"/>
    <dbReference type="GeneID" id="30179878"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120095">
        <property type="term" value="C:vacuole-isolation membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051365">
        <property type="term" value="P:cellular response to potassium ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000817">
        <property type="entry name" value="Serine/threonine-protein kinase ATG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048941">
        <property type="entry name" value="ATG1-like_MIT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022708">
        <property type="entry name" value="Atg1-like_tMIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12063">
        <property type="entry name" value="ATG1-like_MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21127">
        <property type="entry name" value="ATG1-like_MIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="33"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="590"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="590"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="624"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ48342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="938" mass="105933" checksum="18251E46E37B6AA8" modified="2017-01-18" version="1">MKTGAGPGGTAAAAANPVLAAGSSETGKVVSHFRIGIEIGRGSFANVYKGVDLDNNNKTVAIKSVFRSRLKNQKLVTNLEIEIKILRNLKNPHIVSLLDCVKTDHHFHLVMDYCSLGDLSYFIRKKTQLSNHHPIIKTVLEKYPSPANSNGLNEFLVVNFAKQLSSALKFLRSQNLVHRDIKPQNLLLCSPAHSKDEFVENHYAGFWELPILKVADFGFARYLPSTSMAETLCGSPLYMAPEILRYEKYNAKADLWSVGTVIYEMVVGKPPFRASNHIELLKKIEKSNDQINFPTDFEISQDLIRLICNLLKANPTDRMGFNEFFSDPVINSNNDIYESMEESLSYSVENEEMYVSEYLVGKDRNENSLPKTEKNVINKKPSALVTASIIKQKAKKQILPNQFERQIQEPLNKLVIEEGNEDELETNGSDKKIKEDPVANNLKSNASNGIVTRENIENRIRPKSMEKSVEELLQEQQKAIKSDPDANGNNTATDPNVTVNVKLNGRDQMLSRRKNKAGNDIIVEKDYVVVEKRNVEVNTFADEISKASKPLKNPERHSFSKQRRYSMSVSPSGALKEVLDYTTERLFGATNTTANNNTGSTNTEAVSTSDRHDDYGPESTPRSSQDKPTEITKIGSSEDKVDINKIILGRNSSFKNTSLNMRESNDKTDQIDGIINKIERFAMMAHAISLYAEIKFDQVIPLPPSSNNNESNHFVDVFDLDDYNRYSGAIGLEKNMNNPEKKNNSTNSNTLQYNSVYRKLCQEGLALYLKTLLFLGEAIKASSEWWAKNKYKSNITPMMGELIQWIRNRFNESLEKAEYLKLRMNRFEEEENSAENNPEPNFICEKIIFDRAIEISKDTAIQELGHATDVNELKKCEIAYSLSVWMLESLLYQPKESDGGCLERGDQDIVENFINSIGTRLLILKSKIDNQSKGTRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQU3</accession>
    <name>A0A1E3NQU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Phosphoribosylaminoimidazole carboxylase</fullName>
            <ecNumber evidence="4 11">4.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_14037</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+) = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2</text>
            <dbReference type="Rhea" id="RHEA:10792"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">In the C-terminal section; belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019580.1">
        <property type="nucleotide sequence ID" value="XM_019160195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQU3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQU3"/>
    <dbReference type="GeneID" id="30176882"/>
    <dbReference type="OrthoDB" id="15425at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000013">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000037">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016301">
        <property type="entry name" value="Ade2_fungi/plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001340">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="110"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="570" mass="61479" checksum="8A7EE5FF5B321130" modified="2017-01-18" version="1">MDSQTIGILGGGQLGRMIVEAANRLNIKTVILESGAESPAKQINANGEHIDGSFNNIEDIRKLAAKCDVLTVEIEHVDTEALKTIQKETGVKIYPSPETLEIIKDKYRQKEHLILHNVAVAESVSVESTASALQAVGSKFGYPYMLKSKTMAYDGKGNFVVKDASYIDEALKVLCNRPLYAEKWAPFAKELAVMVVRNPSGEVFSYPTVETIHKDNICHTVYAPARVNDTTQAKAQLLAGKAVSTFAGAGIFGVEMFLLPDGELLINEIAPRPHNSGHYTIDACVTSQFEAHIRSITGLPMPKNFTCFSTPQTNAIMLNVLGFDEPNGELELCKRALETSNSSVYLYGKSTRPKRKMGHINIVSGSMSDCERRLAYIEGGNVSPESIKSTGTIPGTSLSPLVGVIMGSDSDLPVMSAGCNILKQFGVPFEVTIVSAHRTPQRMAKYAIEAPKRGLKCIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSTLDGVDSLHSIVQMPRGIPVATVAINNATNAALLAIRILGAFEVKWITEMENYMNAMEDEVLGKAEKLETGGYEEYLSTYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR05</accession>
    <name>A0A1E3NR05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein CSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14074</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019628.1">
        <property type="nucleotide sequence ID" value="XM_019160211.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NR05"/>
    <dbReference type="GeneID" id="30176898"/>
    <dbReference type="OrthoDB" id="10051416at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006113">
        <property type="term" value="P:fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029636">
        <property type="entry name" value="Csf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056779">
        <property type="entry name" value="Csf1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048636">
        <property type="entry name" value="Csf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085:SF3">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25038">
        <property type="entry name" value="Csf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21678">
        <property type="entry name" value="Csf1_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Csf1 N-terminal" evidence="2">
        <location>
            <begin position="126"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="2">
        <location>
            <begin position="788"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="2">
        <location>
            <begin position="1275"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 C-terminal region" evidence="3">
        <location>
            <begin position="2051"/>
            <end position="2974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2200"/>
            <end position="2220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2975" mass="338319" checksum="6BF79DDC3AC0F976" modified="2017-01-18" version="1">MAVVTKSELILVHSAVLTWRYWLQFVRTSQFYVQEKNLNNTVNRSLPSRFILEVTGMEVFVYNRSCAYDDIEEILKSEARQHQEKHSKHNHKHFPNTQHKSHDGKKFSEDVTSSSPSEHATYSLKHRRNRSSSSYGASTSTIPSAKAKKEEDLNADYSQEKAENESFQNNKFKKDEEDQSTITTTEVMDSTFLDFLPFEVKISKGSFVLGNETTSSLYVASFNNMSGQIDASLPGSALDYYRTHYDFTVTDLKIDLKTNVTFKDIDTLEKKIQSINNKKKTKNFISMISGLGHSINALHVLFNKHKKTSFTSADSELQKNLNNGIEEWHGLERYLTTVSSSDSDLDSRMYDYDKQRMIDTEYAKYSNILDAQRCKINYYYDSQGLVPAEKVSSDCIEDPDIGNKDIPPMFGVDITLSGATACYGPWAEKQRGSLHHLLFPPLYRNSVPFKKLKTGMRRQYVSFDLSIQCADEMVFRIPHREESKDINFLKTNIPTLRHFGWLDLKVAEGSITDVSVSFISTVEKGVDNKINAVFVKPEISTSVNHDILFEADEHILNASVAFPLEWNSLADWKFENISKNVNIYLLREHITLLTDLFSDFSSGAPTPYELFRPFIYRFNWKLYDYSIYLNINEQNIINNPLDKTINTYLTFKGASLHLTTTIPITSVYKKSNTVDYILETSYFDLSIEHPTSSTFSNFTQTEEIGNANNFKMEGSYTYFSFLEIDSVDTIIMNCTCDDTTVKVYGFAVKYFMSLKENYFGDSTHFQNLLEFRDSFGTEEKDSNAFEGKRMKNETDFMFSFCVNNGCLVFPCHLYDCMSHLALHFDDLDIDIRNNNYYMDLQADFSEIKGRYVENCDESVIFANTKSKLEFEPQIFIDGLSVHSIRIFGLPPAEPTYFCRWTFDSGDISIISEPIFLDAISRAGMSFKFGHKDLENSLDLPQASVLDILNLLFKCPAVHITLKSLDYVFKVSLIDISFKNSDQPTPCYNGLIDLIIREVLVECYQNKIQLLKVVTSLEVKNFIQNRDSFEVMKEQSMHLKKHDASFHRTPFLIPEFAKDRKYYKSFNSLISSLYLPDPPLPLTSESLDMIIDSFPLNVQKKLFESFSSLEDNDDASEEAQRFSSNLEDFGILKGLDPNCEYDNFPIKLEKLEIFISPAVAPILVDIISKMTDFNMYSFLDELQTQFITFFNFRKKNLVMRCKVECPLITFKMADSINATEYLFVGISDLVFAFSRSDVASSSAMNIYAIFKELNIDLIKDMEDAVLITFYRLLFKQSFEEKHISTVDLENLSIFVDPAYAPWIIDWGYELKDLIGQASAKWKAFQKDKHSAGIELLYDLSRGGIDFNISHDPPCITKPSYITGFRKNHIRTDGNWMIIPRLRHVLQNLPLDWILKKNKMFSQKLWEAPKDAEDEVSIIFGNWRCWDNHKTKDNFIFKQVFDSEIEKRAEVYNTVKVTLKNFSVGIRPFTNAIIVSDVSFFLNEEDLCRKVKEFAMPLLDRPIEKSLDITFKVQSVATSFTKIHRLFDVLYPLTKEIKATLSKHTAVGQDLDNDDDMGSTASLNDVARYEAATPTLITFNFSLADYSHSLGIDESILVLYGQGIDLTASLIKAEEMLSCTLNIKNQFLATELHVGRIPIFELNCESHNTTLINTGSFDLGKTVVLISNEVVSFNFLPDTPHLVKVLTLFSEKELKSLMSVIRSFKESNIGSSLSAASLPLTSSSSGNAVIEKLKSNLFSKIRMDISASISVSKFSFHFEMIAPFFTNFNMTEPVLSLKLGSFGVLAEFLMERSDWFVGSQSKKSVFEYVTTSFDKLKFSLSGHYKGGIYETLVTLITGIVRINLAHNNLVNVLSRGKSDTELMLKNLDSLKAAAKLLGRAASMSTNTSSSIPSNTSKLDDNLFVELSNIIHLFCDINFGNVIATVNLNGNKFHLDCIKPKLNVKTFDPIINKFSPHGKITLPSTRIAMGLNGVHGVSTIFDIRSCVEVRNPDNVMHKSQRFDVRTDYCRLVLNPHIIDELIEAYGDIAILMEPSESRFVQNSEPIDLDEKIDSILSYISINIVAKNVCFGWLFDEEDILYPEVAPGIIIGFEDTLISCAKDGGKLQTRGMYISAAHGFTPSTFYSANSEKSSDNRAYLPLFNLIYSIESDSKMRNFRSQVNGDKIDFKFQTDIISITEPLWLSINSLQEKFMYVQSVVQQKRDKKTKRHRQPSDEPASKSPLSPFSNTFKMTSISCVFNFAGASFFIYNSHIEINGAIPILSLQSPRLYAILKYSHDILALKKHTIFFSALISETNNKLSCLCVPVLQDIMKGFQEMMRRSNGKNAVVSKETSNNGIDIVNLSEKINVNFSLKIEPQSLALTCEPRANVEAEVGLEEIHLIIKTDQDFLSVVLSIQSLRSELKHAYSKVTSGSVHAKRLTVNGAMTSIEGEKKFCTVAKLDEAEGFINIQQRQDLDLFKDLWLPSGFNNSLLVKKSKSVDEKKTFASMLREVSTTTAFPWVLSLLVAKVSAKVDLGSSLGELNVNIQNFMAVSTKSMEWDQNLKLELDMLQIESRGRLSGMLKAENTRMTSAISWKKHGEVLVVPLVLLSFGFGCLQTKISLDFHPFFILEVVKFGVTIYNQRQKDTHDNLKSSLNVESIKVFMTALTASNFVDVYTIGLRIRQDIKLSYRQVLNDAQLGINENMSKSVEANTLEREESKNTVELTTLSQTFLSMIEKLKTYLEVNIGMLEIQVFPSSLLDSQALVINIGKERAMFYQNNVLDTENRLSLDLADITVSLSSFKNKPTAEALNDENSIKTYLDMVSTPIADNIFVFPTLKIMMVTSQNKGDNVIDYKYVCKFGGKVDIKWKIGSVYFIRQMWYSHATTLSNRLTALRIYTSDEYSGEAEENYKESPFESVNLEDRLKDVESDKKFTYNAIEEPDIETPQLKDLGNATPPLEWFGLHRDKFPNLTHQFVIIGLQKIIREVEQNYSKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR34</accession>
    <name>A0A1E3NR34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Efficient mitochondria targeting-associated protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14087</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM97/sigma-2 receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019644.1">
        <property type="nucleotide sequence ID" value="XM_019160214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR34"/>
    <dbReference type="STRING" id="763406.A0A1E3NR34"/>
    <dbReference type="GeneID" id="30176901"/>
    <dbReference type="OrthoDB" id="433124at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051987">
        <property type="entry name" value="Sigma-2_receptor-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016964">
        <property type="entry name" value="Sigma2_recept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204:SF1">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031032">
        <property type="entry name" value="TMP_97_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="3"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="168" mass="19886" checksum="6DD421BF9E86B183" modified="2017-01-18" version="1">MISDSVYFYYFLVHLPITVFMDATFVIPTEYQLPIQKQLSLFHIQQNKDFLAVEAPLWVRLFVIWELVFQLPFFVYAAINYLQNGRTKYSERTWPMFLLYGFNAGFTSLVCLIYVWTESGRHGLTMGETANLLGLYTPTTLIPFYMMYDFWVRIADQLQSVEHDKKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRA4</accession>
    <name>A0A1E3NRA4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_56843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019738.1">
        <property type="nucleotide sequence ID" value="XM_019163202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRA4"/>
    <dbReference type="GeneID" id="30179889"/>
    <dbReference type="OrthoDB" id="3993599at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="404" mass="45612" checksum="0E78810A2872D893" modified="2017-01-18" version="1">MFFVAQPEYYRQAPHGLADLNELCLLNNLQRQYVYRQQQQEKRQVQKLNQPEVFLTQDLENYYVVLTKRVNRSNYAYMHRFNGYALKQVERSLIVRSTRDNFYRNINLPSNIDLDRDITYKILDNGYKMVVTIPKRASYQIKTTAFGFPDILDGLSLKLLPSTDRLVRNGAAVVGAEKARKDDRGEKVGRGSESCGIRIPISDDCINTDNCLLLPRQHEEVDEGDEVDDFDEHADQNKQHLLTDTTRQNASPHFTLRQVGGEGGKAIEGEVGYEREGAGDRTLQRAEPEEKRAVEEHKASATARVPGPVSSASDVSDSGVIGGESCESQLGRDVRTSERSSPDFLKNYTTLQRKTVFLPNTTTEMEVDSEGEGEGEGEEEAPVPVPVRRTMSPTLEEVVDEEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRH8</accession>
    <name>A0A1E3NRH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14222</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019805.1">
        <property type="nucleotide sequence ID" value="XM_019160279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRH8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRH8"/>
    <dbReference type="GeneID" id="30176966"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000018">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="440" mass="48578" checksum="09416618170C95D9" modified="2017-01-18" version="1">MILNRYNQNLNTGDNIMASSTTGTNTSHNSSSYTALDVLLNSSASDTSVTSSHSINPTPMSSEYSLSNRSSLADVSFNALESISNDINNLNSLNTINSINSLNDSFDNDASTIDVNNINNAENNPSSVMNNLWNSTSDNSNLPSNSTFINWNPNYLSSNPNNNHNDNPILSKPTSPVPQSFATLRNSKNLQFSSNDPFLPADLKTSDMATINNTSLDDAIYKINSSESLYKCLSNHLLSNISKATTNTNLNNESLLNNQFLNKPAFPLNNNTMAGYSWNANVGLLNISDDLNNHQSSSSYSNSSTASSVCDDLINHSYYQNHLNNINHANNASPNNTNIHHTNSNHLNKNKTDRSLFKTELCATFQKTGFCPYNDKCQFAHGLDELKSAPKSRKWKTKMCKNWTEKGHCRYGKRCCYKHGENDDGSSVNSLFPPHVVLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRS4</accession>
    <name>A0A1E3NRS4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019899.1">
        <property type="nucleotide sequence ID" value="XM_019160324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRS4"/>
    <dbReference type="GeneID" id="30177011"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133477" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7266" checksum="11D35D1C9B789F76" modified="2017-01-18" version="1">MCVMRSHGGIFLFLFLLFPLRWYCSADADVESAFALFSYTDAFPPAILYDGARYEHEDFFGGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRU3</accession>
    <name>A0A1E3NRU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sphingolipid long chain base-responsive protein PIL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_30488</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019902.1">
        <property type="nucleotide sequence ID" value="XM_019162638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRU3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRU3"/>
    <dbReference type="GeneID" id="30179325"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097446">
        <property type="term" value="P:protein localization to eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35188" checksum="AFF03C6CECEDA0C9" modified="2017-01-18" version="1">MHRTYSLRTARAPTASDLQNPIPVPPSSTKGPRFFGPGGIASSFRRTTAGSFGPDLSRKLAQLVKMEKNVMRAIEICSGERRDAARQLSLWGADNEDDVSDITDKLGVLIYEVGELEDQFIDRYDQYRITLKSIRDIEGSVQPSRDRKHKITDQIAYLKYKDPQSPKISILEQELVRAEAESLVAEAQLSNITREKLKASFSYQFDSVREYSEKVALIAGYGKALLELLDDSPVTPGETRPSYDGYEASKQIIIDCENALATWTFETAAVKPTLSFKVGGYEDLNEDVHNLNIDDDEEEAWNEEQEQEPQAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV4</accession>
    <name>A0A1E3NRV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Autophagy-related protein 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14392</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019970.1">
        <property type="nucleotide sequence ID" value="XM_019160350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV4"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV4"/>
    <dbReference type="GeneID" id="30177037"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="307"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="50905" checksum="1C4161FD0F30306A" modified="2017-01-18" version="1">MLTLLSFNQDRTCLAAASASSFAIYNCDPFDSCYRCAQHTVTIAEMLFSTSLVAIVDPAVGTSCLRIINTKKNSTICELAFPSRIVSVKMNRKRIVVFLATQVLIYDVSCMKLLQKIELSHPCVEANGTQHMACDLSSSDLSVLAFQQFDPLASSAGANGAGHANGTGNGGTNSSHGAGGEPYRDGAESDNLGSQSGNAVIFDALKLSPLCIINCHKTALQILTLSENGVLLASASKKGTIIRIFNTQTGSKLCEFRRGSLPASICSLSFNSNNTILACSSTTGTVHFFSVPDSVAQLGVSKDFASAPLSTPPTATMDSSVIDTPSTTSSSPSSPNKTVPVIPSNELPNSSSLTTEETDEVQKLMASVDNLERGGKHTSALQKTKNLTSMLWSQSKQYLPVQISSMLEPKRDYAFIRLPATGDSMIGLLENTCYIATADGMFLQYNIPSPSVADALSTTNTITNSPIECKLVKHHQVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRW5</accession>
    <name>A0A1E3NRW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-binding protein REB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019958.1">
        <property type="nucleotide sequence ID" value="XM_019160346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRW5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRW5"/>
    <dbReference type="GeneID" id="30177033"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051651">
        <property type="entry name" value="DMTF1_DNA-bind_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380:SF2">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="638"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="642"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="760"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="777"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="224"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="411"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="516"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="732"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="862"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="516"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="862"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="963" mass="107207" checksum="25884AC424D758E2" modified="2017-01-18" version="1">MSPSDHAATERPVSKGARDTADTNNANENGVIPVDSVIRDTGTVNKDNNEEYNEGSTINVSDEILTVGNFVESETQGGHAHSENPKGANDDEDDDNVEDIGKFSIESISNISNIDHISIDHMNAIDNIDNIDGIGNIQIHGQNIINEQQDFPTIEVIKTVQRNRKKRQFSTIEDEEMSRLEFQQWTSGFLADDLEDPNVEVSIDDVEYGFPQTTAAHFTDVVEDQSNVGSGKRRKVDRKNQRKTFKSEKEQTAKLRHSSEGQKDNIEKTAASFYIKHTEEGDDEVEMNSKIGVANSQHNVPSNKDHEIHKIVNETKNSHHQLDDHLVEKEEDNVDPELANIDTNAFVHDAMLDARALVSLNSLDYLRNRNEGKSGFEGEDSRGQEEVDDENLAVEAVNRAVNQAVAAVHAGANARGDEDKSDNSQVDGDVNDLVNDNNNEPDADVIAAAVVAAENSVRKRIQSVGLPSSVSDGDKQLFEIFQKEHNKSVGDGEDVVGATDPNAPLVTRHGIKNNRVLSKLGSKASSSKKSTKKKSKEVKVEESEESVLEKEKIAEAISKAQKLISEQPKGRSFTKEEADAIEVFIKEYEKIHDMTHEKFLQRIWGNERKKDKFWEILHSVLPNRTRSSLYKHVRRTYHIFEKRGVWTADEDLRLAQLAKTCEGKWRLIGEELKRMPEDCRDRWRNYVKCGSKRNVNQWTIQEEFKLKSIVARVLEEERRRLREEGLRNAAAAGAGAGAGAGAVDEKRADGSGPAGGGSAQESRLKQAREAQVSPTINWTTISELMGGTRSRIQCRYKWKKIMKNESVKKLREMGHDTKLWMLYSIKRLSENDPNIEANLDWNALALSVPEAYSFAANCAESGNVSTGGSPTTEPKQEQQKEQQQQQQQDNEPPEPALQETPSPLAWNGVDLATCFQRLRTTVDAKGKGFVETIDLLIAALSAASYVDRLAEAEGDVGDAGDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRX3</accession>
    <name>A0A1E3NRX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein pan1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_14319</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019897.1">
        <property type="nucleotide sequence ID" value="XM_019160321.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRX3"/>
    <dbReference type="GeneID" id="30177008"/>
    <dbReference type="OrthoDB" id="2015333at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000349">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein PAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013182">
        <property type="entry name" value="DUF1720"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF173">
        <property type="entry name" value="ACTIN CYTOSKELETON-REGULATORY COMPLEX PROTEIN PAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08226">
        <property type="entry name" value="DUF1720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="18">
        <location>
            <begin position="316"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="349"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="18">
        <location>
            <begin position="640"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="673"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="742"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1038"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="801"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="743"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1043"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1058"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1094"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1134"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1168"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1179"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1208"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1218"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1242"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1290"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1335"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1377"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1406"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1466"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1502"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1549"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1572"/>
            <end position="1612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ48784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1653" mass="178908" checksum="C0B485ED4CC6593C" modified="2017-01-18" version="1">MYQQQYGYGNNQGYPQQTGYAGNNWSTQPQPPPQQQQQQQQQQQQQQQQSTPSQGPQYNHLQTQPTGYGFGTQINSQQSLPPLPVQSQFSAQQTGNWNQLPVQQQQQTQQTQQQQQQQPPTSFQPQQTGMMPQRTGFQPSFQSQPTDGQGLMAQRTSYLQPQQQQQQQPNQQLMSQATGWNQQAPPLTSFQAQPTGGLLDQRTGYQQQNQQPPLTSFLAQPTGTLLNQRTGYQQPLMSQTTGWNQQQQQPATTFGSMPLQPQATGWNTNPLQPQLTSGQPLTAQPTGFISSLTSMGSQNNDLVLPNIRLSFITARDQERFETIFRNNVPKGENAMDGNTARSILMKSQLSAVQLSQIWELADTNKSGSLMFPEFCVALHLANMSKRGQSVPFELPTKIKNEVTGFVDAINFNIGIKNDRSTNNPIAPQATGYSSAPLNAQRTGLTPLTAQQTAGLVPIQPSGMLTSQPTGFFSQKTGGLMPQPTGTAPPMTSFMAQQTGGLMPQTTGFQPQTTGFQPQTTGFQPQTTGFQPQTTGFQPQTTGFQPQATGIMPQQTGLMPQRTGLFPQQTGMLSQQTGLQPMPTGKPGQWGFVSTPTGGLPGLDMMQSHFMPNAQSQSTLLQNRMGGSSASNVTWAITKQEKLIYDNIFKQWDKERNGFVTGDVAIGVFTKSGLSFGDLERIWTLCDQGNKGKLNRDEFAVAMHLIYRRLNGFDIPSYLPPELVPPSSKVLENTVDSIKDQLKKQASNGGVGSSMNVKPGVNRSGTTYKNDDDSINYVSNSRHRRRSKEDEKSSAFSTKLSINDLKKQIHEKKILLSALDAQDNDLSVDHEEQKTLNEIELLKTKIKSLQSELNSSNTGNVDSIEEKEQLNAKLNKFADKVPQLMDAIGQMDDKLKAAKIEIFRLKLQKENPSGVEFRGTGANGEITEADKRIARQKALLQAKMAKLTGKPAPNFDAFEDNEAQLNEEIVKISKDFDEQKGMVKDISASIKSLISDVSDSLNLTNSMSVGHVKWEKKEGIQSPEVSEFIDYLNSTKPAHKTSISAPSSLSNPYLSSTSGGAKQSNSVLPSSQDTKVVAATTSAENSKPSSGLSAAEERAKRIKEKAEKRMNERLAKMGIKREKPTPKNGNIASEQQAPSQSPSQSPAPTPAQTQAQPPVARNEVPPAQPAQKAPAKSSAPPAPPAPPAPRSTGPTPQRAAKPSSFESDSSSEDEDDDEYKALLEQKREMERREKARKERKKREKEERLAKLKAEMAAMKKEEEQDSDDSWDEPAKQTVPAPKAVAVGNVQPPVSNNSKEASNHSNNPFASMMGAQSTDLKPAASPVEQKQEAIQAPSYNPFSKLQKNNSNSTSEVASVDHDKLKAQRASQMGTGNDDGWSDSGDEASDDEMPTATKQAELASMLFGGNSQPSRTTSYVPPAPAPTPAVDGMFENTETTEVDQPASQEPVAPAFPPPPHSPAADEPSQPAPPTLSQPPIPDAIPPIPVASPPAPLLSMPPVSNEGREDSEFYDAKSEESSFIDPTPPSSPESAFTAPPLPTNEVPLLPSSDAPPPPPLDAPPLPTNQAPELPSTIPPPPPPLTSPPAAPELPSLAPPPPPPPAFSAAPVPPPAPTGGTPNINALLGQISLGTSLKKVDDSEKHISSGAVVGKVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS99</accession>
    <name>A0A1E3NS99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 21">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="15">IMP dehydrogenase</shortName>
            <shortName evidence="15">IMPD</shortName>
            <shortName evidence="15">IMPDH</shortName>
            <ecNumber evidence="15 21">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PICMEDRAFT_14444</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15 21">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15 21">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer. Seems to be able to form heterotetramers composed from more than 1 of the 3 IMPDH gene products (IMD2-4).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15 20">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="15 21"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020037.1">
        <property type="nucleotide sequence ID" value="XM_019160376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS99"/>
    <dbReference type="STRING" id="763406.A0A1E3NS99"/>
    <dbReference type="GeneID" id="30177063"/>
    <dbReference type="OrthoDB" id="416622at2759"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000007">
        <property type="entry name" value="Chromosome 19 SCAF14664, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15 21" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="5 15 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15 17" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 15 18" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 15 21" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="120"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="183"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="15 16">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15 16">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <begin position="277"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <begin position="327"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="367"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="390"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="414"/>
            <end position="418"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ48924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="522" mass="56322" checksum="A44D6DAC9A1AE39B" modified="2017-01-18" version="1">MSFLDASKATSYLKEYAEKDGLSVAELMDSKTRGGLTYNDFLILPGKIDFPSSIVSLQSKLTRKITLSTPFVSSPMDTVTESTMAIHMALLGGIGIIHSNCTGEEQAAMVKKVKKYENGFINDPVVISPSTTIAEVKDMAQQFGFTSFPVTESGKLGGKLVGIITSRDVQFHEDSSSPVSEVMTKDVVTAKVGISLLDGNDILRTSKKGKLPIVDAEGNLVSMLSRTDLQKNQNYPHASKSPITKQLLCGAAIGTMEVDRERLSLLVQAGLDVVVIDSSQGNSVFQLEMIKWIKNTFKDLQIIAGNVVTREQAAELIEAGADGLRIGMGSGSICITQEVMACGRPQGTAVYNVTEFASKFCVPCIADGGIQNIGHITKALALGSSCVMMGGMLAGTTESPGDYFYRDGKRLKTYRGMGSVDAMQKTDTSANASTSRYFSEDDKVFVAQGVSGSVLDKGSIIKFIPYLYNGLQHSCQDIGVRSVEDLRSSVRSGEIRFELRTPSAQFEGGVNNLYSYEKRLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSB2</accession>
    <name>A0A1E3NSB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_9490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020102.1">
        <property type="nucleotide sequence ID" value="XM_019165097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSB2"/>
    <dbReference type="GeneID" id="30181784"/>
    <dbReference type="OrthoDB" id="441812at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="64317" checksum="3644957FE7194064" modified="2017-01-18" version="1">MVLFAGEIKNKSELETWREKLGIKVNPNLRIEKTKERGIGVFYTCETQNESSERIELMRIPHTSSYNIYTLRKLVDENLSAEDKAIVKRTLSVIFARSRGSSESLILIAYFIGFLIVSKKRNADSYRNSETDEFKQTMGTYLSVLLDTTIGNLYGDHPDILEDFLSAFPGNVVLKNSIVDITSGLWDEITDTLNEEFLEKDEAMKVEEVLQLCGAIRSRVLEIPREVEGDQEDYYVDVTLVPILDYANHDNEKRNAYFDVDRKSQEVVLYFEPNKVASERDQCEIFISYDEYEDLHRMFVNYGFFPLNKERRTVIEIPIIGYCESKLMSDFEITRRLYCIRQTPNVQFEVLFDAKGGVKSVKVLDDEFYSYLAFKDDLPWNQYEKEDDEAMNELNELNEGKKGGEVDMETEMELDFARGFQRSVAIESSLSGEETSRLMKILWDYVCEFFVKFSAKCRLFNEIIAEYEFAGNEGRNIVKLLDLYIAVCDHVLGKGGEADRILDAGSVFGKRSKDRSSRNKTASGEDADEEDLQGFLASRMVPVYNYSASADGVEIGELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSE2</accession>
    <name>A0A1E3NSE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Autophagy-related protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_75262</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Autophagy-specific protein that functions in response to autophagy-inducing signals as a scaffold to recruit other ATG proteins to organize preautophagosomal structure (PAS) formation. Modulates the timing and magnitude of the autophagy response, such as the size of the sequestering vesicles. Plays particularly a role in pexophagy and nucleophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Preautophagosomal structure membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ATG17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019711.1">
        <property type="nucleotide sequence ID" value="XM_019164935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSE2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSE2"/>
    <dbReference type="GeneID" id="30181622"/>
    <dbReference type="OrthoDB" id="1937984at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007240">
        <property type="entry name" value="Atg17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28005">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28005:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="7">
        <location>
            <begin position="15"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="434" mass="50985" checksum="63ED9B2285801A78" modified="2017-01-18" version="1">MQTIGTIKNDAIIKLNEAKRICFVANQNLADSFDQLARKEKLTLELSFIVKSLRNHLRIFESLKRSVSLCISDVSRKKSLYFVESKQKINRLEKIYDQMKSIKVDPSLCVTKNCNENRTLYDYINNDHISDIISKMDNHFVLIDSLVDSKIMENIIVRFQNESNYLYNEWESINSFYQKYFIRKENEKELDGLVKNNTELEAEIIDILKDLNTHLDNCINYELQNSKNDAFYDLMQKQSAQKAASMDILQSNCDIISQNCKDIEKFVSIFEDFRNKLIKCFKEIKDFSANVLEKQVQNNLLKITREIKAHFDTLNDYKEDIIQFSEDSLDFIDSYYYLVLEVDRRCALNKKVQNLINDFESELKTLQENDSIKRNQFMSDHAAFLPQNLADFDIINSKFPQLELSYTLENLPSLRKSIVEESINKLKASHTDIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSK1</accession>
    <name>A0A1E3NSK1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70654</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020192.1">
        <property type="nucleotide sequence ID" value="XM_019163699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSK1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSK1"/>
    <dbReference type="GeneID" id="30180386"/>
    <dbReference type="OrthoDB" id="10265211at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020795">
        <property type="entry name" value="ORC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045667">
        <property type="entry name" value="ORC3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040855">
        <property type="entry name" value="ORC_WH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07034">
        <property type="entry name" value="ORC3_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18137">
        <property type="entry name" value="WHD_ORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 3 N-terminal" evidence="7">
        <location>
            <begin position="6"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 3 N-terminal" evidence="7">
        <location>
            <begin position="271"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 3 winged helix C-terminal" evidence="8">
        <location>
            <begin position="700"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="247"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="568"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="860" mass="100892" checksum="A4A0B02CDF884EB6" modified="2017-01-18" version="1">MSKVNDFIDSQKTIYELKPSKQRRIPSIPDGCKEGRPLNDSEYEKAGKVPFVRLLGGKEPLRNVKIRHELYQKNWARQMHAIDSVLSKTDRFKFRSLRDFLSRRDTSSNQNDLLTTALLNLGSNISNHDRLLNNVCRYLREDENVCLVKINPNVCFNIQRIIKRIEDTLACKVHDIVSGKLSNHSKKNERKNNSRKRDRSGGNIQKAGTPTPRRKKRKHAESGSGVSDAIKIEDGNEGYDTILIDYDDDIDDGDYRNDEENSNEDENEDMEDQVDEEEEEEESDDDFDIELNNDFDLLPKKIEKDKYVDIEDMLQVFEKAGVRLIVLVQNADAMNSILIQQTLMLLHRYNQISEVYSIIGISTPFIIFQEKVPKILIGKLRTRSFAVDNSNEAINQIMEDLLLNINETYNSLIFDPKLVLKFLYRRDVMSIQQFSNYMKLIYMRHYYSQPLSLFWTNDFSKIDLNSIYFKIFKTLPSVMENSSELDKKHLVGIVENDVQVIGDLLRTNLNQLINWRFNFRNLIDFLNFAQASILDVKIWSNNLELFQLLFEKYYELRDAKEVWNDTSEVDEQEIDDEDEKKRNKKKTKSKYSNSKISHTSLFKFLIELWDVLKNTSHEKVESFYDQLCSDEQFTFITKSERFPKKCPTKGAINGFIEVIQLALRDQVCELDLDGQAFREICVVREDAVWKIHDGFEPCVRENCLKNLDEPGKVLFNSRHWVFNEQNETQKTEVKMFSVIEPILCEMYRIFKETGLVMNVYDFYQVFKNSIVSRRELITLMREKMMSGEHVKGLKHEEIEKLLEILDKIEVELEGGEVEEEWDKMLLSWFLKSISEFQMLGLLKEGKNKSQSVEKSLWRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT10</accession>
    <name>A0A1E3NT10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA stability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an essential role in initiation of the G0 program by preventing the degradation of specific nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019816.1">
        <property type="nucleotide sequence ID" value="XM_019163612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT10"/>
    <dbReference type="STRING" id="763406.A0A1E3NT10"/>
    <dbReference type="GeneID" id="30180299"/>
    <dbReference type="OrthoDB" id="5949865at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF6">
        <property type="entry name" value="ENDOSULFINE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="269" mass="28975" checksum="2A3C977B95D30097" modified="2017-01-18" version="1">MSLAVGYSDSDSDSGDAEPVPSPAVLPPLPPPPPPPADIMHKFSKVPLQVTRKRMASGSVIRSDSSSKETTFVYLPVTPDAGLLARITAILDGVSSSLDGHTEQHLPLLNLSVNELTGGASVLHVSLCHNMTLSRPDLDAFIEHHVADMSTNYSPNDPLYTPPETGVDLSQLSPQELKLYKMYGRLPKTNDILRGKLKDRKFFDSGDYAMSKAKGRAPDTEQVGVLNPLKTPEIENVARINRNSVAGSVSGSASLSKKSKLEDDMTDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT16</accession>
    <name>A0A1E3NT16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70761</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020313.1">
        <property type="nucleotide sequence ID" value="XM_019163731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT16"/>
    <dbReference type="STRING" id="763406.A0A1E3NT16"/>
    <dbReference type="GeneID" id="30180418"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000146">
        <property type="entry name" value="Putative two-component response regulator SSK1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="563"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="265"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="504"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="724" mass="80048" checksum="6CA9AB6E4BCCFD7B" modified="2017-01-18" version="1">MFSLHRKKNHDGVSPKVMPYVNQQHLGSIVAPIPMSKSASAQISHLPTSSPGARSDQHSLRGASPVAYSDSQGNNNIYYGGLDTVHSATPSGVQSKKNSMEVSSQDSQLKHNQYSHLHNDKEAAEIDSILQQQTYNILPQGKRNISQSLRSAQSKTPVEKRIWVRKNSHSATTITVGPHDIVDDLKYVISSKFPTTLGRRFDPSDLVIKLIITPDTRGFMNSSPSTSVNQFSLSKKPLLHDGSHSPMTASSGYSRLMQPASSNDLLRANTPISPDSFPNQKYAATSSDSIDTPHTRTSPRVLILEPDVLVWSIVDKYFPNGMNMSDAFLVDTSKSPSEDSFKFDQRNTNQQYLARNLQTIDKYDSNISSLEMNQAGSTSASLNKTAILGDERVPPPRLRLPNSLDYGIGQTPQSSAVILFPRDVRGDNKSLPPASHPEMIPPPPSPQNLKIDRSDPPIQIGKNYHSRTNSSELQKKLNLTLNTSSTSDEVNETNEAQKLTLSDTTLGSAPNSASTITPKIHLDPIPDSSSTLKKPETKLPVIEKKPKAIEKKHGISKILSHINVLVVEDNLVNQKIMARHLKSCKVQFQIASTGKEALEMWKKGGFHLCFMDIQLPVMSGIEVTKEIRRLERLNHIGNFSGHNIDEANDYKESDDVLDLSLFRSPIIIVALTASTGASDQQNALAAGCNDYLTKPVQLKWLKNKLTEWGYMQALINYDYFRNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT58</accession>
    <name>A0A1E3NT58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020450.1">
        <property type="nucleotide sequence ID" value="XM_019160539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT58"/>
    <dbReference type="STRING" id="763406.A0A1E3NT58"/>
    <dbReference type="GeneID" id="30177226"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000001">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="71"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="209"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="329" mass="36924" checksum="D14DC5F673D1052B" modified="2017-01-18" version="1">MIVSSILLISKRSSILIKSSRRMLSVVPPASVESFYQKHKDTLIPIDSSWYMPNVPINAYHEFMKKRFSSGTVFFDIEKAKDHSNKYPHMLPSKEDFEKEVSELGIKNDSELLFYDQQGVFSLCRAAWMFEVFGHNLSKIYILNTYPAYAKSHSDPNLVNKVHDKKTLLDNKLVSSPSPYPTSEYHATFDSSKVVLYEQLLELVENGKIGSEYTLIDARSAARFSGGSPEPRAGMSSGHIKNAVNIPFSEVLTPQKAFLSNMTLQSLFAEKNINHNKPIIVMCGTGVTACVVRCAMQLAGFDSSNISIYDGSWTEWSQRAPKSLIVKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT86</accession>
    <name>A0A1E3NT86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_14311</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019886.1">
        <property type="nucleotide sequence ID" value="XM_019160318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT86"/>
    <dbReference type="STRING" id="763406.A0A1E3NT86"/>
    <dbReference type="GeneID" id="30177005"/>
    <dbReference type="OrthoDB" id="271164at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06861">
        <property type="entry name" value="PX_Vps5p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000013">
        <property type="entry name" value="Putative Sorting nexin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000022">
        <property type="entry name" value="Sorting nexin 3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037868">
        <property type="entry name" value="PX_Vps5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="406"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="301"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="812" mass="91553" checksum="0A6DE5B1EFA4E839" modified="2017-01-18" version="1">MNSDMESSNVWGESPLSTEANSASVLYTAASGADTAYPNPYNAFSADDTMDTKVSEDDVDNIGHAKENRDNHAKNEHTTDHPWDADPQDAQPYKSYSPIYDNVSHIDEFKIQNELAQTMKSILLNEERNAGVEGDSDNDNEQTEDSHFDQTDHVHYVDNIHRKSDMGNPFLASSIKNNDGDAVGHIDINLLKEKKNELLSSLTKDTNTRGFLEDNEDDKINTDELFALSPAKTAMGLSNGVDDSPLNNLLSLAGSNAAGAAEANDSNSSTAAASDRLSKSKGFVSPNRKIKILRPRRIAKSVLRSEPSTSVKDLSSATDSSNLTPVDPLSAPLQNDTPNGNNFDIVSVSPRSRKQKFITESEAPLFNVNRDQVLENSRHPISPVSSVSVSESFSPSKYHLEDEKGEKFDITVGDPIKVGEITDTHVVYTINTKTKSDLFDNQDTTVTRRYRDFLWLYHQLLNNHPGYVIPPPPEKQVYGRFNDKFIENRRLALENMLNKVSQRKVLQTDPDFIIFLRSENFTEDSKERETIVRHDPDLKIPASVDENTHLDGPMNTMAQILNATASLGISESSSGGGFFSSLIGLNAPKYVEEDPFILEKQAYIDSLDTQLRQLTQSLDMILEKRDELTLSLQDVTTVIQQLVDLEVNVEITDILSNFEELQSKVKEILERGNLSQVLTFGSTVDEYVRLIGSIRNCFENRLKICNSVATLKQHQEKKEHSLARFRAKNQNQPEKIKRYEDELSKINSVLDKQVLFKDEFDKIFKSELSRFEFGKIKDFKNMVEIYWESLIENQKILIELWESFYDKCVFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTA2</accession>
    <name>A0A1E3NTA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14805</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020452.1">
        <property type="nucleotide sequence ID" value="XM_019160542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTA2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTA2"/>
    <dbReference type="GeneID" id="30177229"/>
    <dbReference type="OrthoDB" id="5541786at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061852">
        <property type="term" value="C:retrograde transporter complex, Golgi to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF81">
        <property type="entry name" value="ER-DERIVED VESICLES PROTEIN ERV41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="6">
        <location>
            <begin position="111"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="305" mass="35198" checksum="033854564AD3B62F" modified="2017-01-18" version="1">MVFLIWVQIGGYIDGFIDHQFSVDEVIRSTLNLNVDIMVAMPCKYLDANIRDVTEDRNLAEEILNFEGLFVPNAFWNMGIQKDVHTPDLDIVLSNSLEADYVTKGVRTNLDAPTCRIYGTVPINRVQGDFHITAEGLGYPGMSGITPADSLNFTHFINEFSFGTFYPYIDNALDLTARKTNQKRHTFHYNLKVIPTVYSKLGHQIDTNQYSIRVYETDEKYAPGIFIKYDFDPIKMTVIEKRLSFFQFIIRLVTIIGGLWVIAQWMYRGMEKFILIAFGKEYYRRGEEKKSGLLDAEPEDDFEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTB8</accession>
    <name>A0A1E3NTB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020510.1">
        <property type="nucleotide sequence ID" value="XM_019160569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTB8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTB8"/>
    <dbReference type="GeneID" id="30177256"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="109"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="411" mass="46302" checksum="F8C0F7900649C345" modified="2017-01-18" version="1">MPFVHPRSQVIDGYGPSEDIGDGGDSPLVPFLLISISFLLMLVMLLTVALIQRCCGSRGGELSDDTEESDLQYDDATEIENDASALRTMSPDEQEIYFQAKEYIKLNGFARMDLAPWQKDMIKEKGVHAWSLDTLSDNQHSVTVENKTEITFNNRNIPISVQSNLPIPFTKDVHYIEFKIFDIPERFRGNTLISLSLSTFPYPSFVLPGRYLHSLAYDSNGDRRHNNPFLLEDLKAGHDIFPTLEEGDVVGIGIKKYSRAVFFTRNGKKLSESKIGGHVKLPRSVQLYPTVGSINPCRIDVNFGQMGFVFIEANVKKWALAPLRGSEVPPPLYTKFNHDVLLDCSDVEDSELPEFEDVVHSGMVRPAEFTDDERGDDEVTLDTIEQPPNYDHLIQDEIREAMGENIDDPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTM9</accession>
    <name>A0A1E3NTM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14863</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the REI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020512.1">
        <property type="nucleotide sequence ID" value="XM_019160572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTM9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTM9"/>
    <dbReference type="GeneID" id="30177259"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF21">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR REI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="10"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="87"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="404" mass="47197" checksum="2C9EC57639CEB71A" modified="2017-01-18" version="1">METVSSAYTCNSCQIQFSLSEDQRSHMKGEWHRYNLKRRVAQLPPLSEELFNTKVTTVSEQKKEEMEADGKMEKLGKGMQNLELSTKQQRRKQREELLEKKRKLLELAKNRLVSNGGRVRIGEDGKIVVEKKVATQEEERLKEEVEEAVRQENENVVDEETLIKEKLAKKVDVPVTKCIFCKNEEFENVDGTYEHLFKKHGLYVPEKAYLVNKEGLLSYLYEKIGYGNVCLSCSFQGRNFQSIIQHMLSKNHIKIPYEAEDEKLEISDFYDFSKTYEVDDTGADGNEEEWEDVAEEDLENDEDIDGEKLYASEYGLHLPNGVVLGHRSLVKYYKQNLKPEKEWTEAEGTVMAAETRNFVKTIDSKAIAQQKRVWTREAKSKDRNDRRAQKFINWQPHYRDELLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTU7</accession>
    <name>A0A1E3NTU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_57588</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020690.1">
        <property type="nucleotide sequence ID" value="XM_019163248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTU7"/>
    <dbReference type="GeneID" id="30179935"/>
    <dbReference type="OrthoDB" id="10542067at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="279" mass="31669" checksum="784B07E02AC2E372" modified="2017-01-18" version="1">MVRVNVSDNLQRPLLDYQSNSKISLKCLPSSSPIRNSHQRLLVFDQATKKSTVERDRISLQVNSSFSSSPIKKISHSVHARKKNNNLGKSEIDPCDFKCTQSDYFEPENELRSNLLLKSVNFIQKKSYTVDESVSSFLNRFNQDGVLDLGLDEVSYGAENNSTFNSNISPTSHKSALKYHISYFSNKLETIIEIPSSEDIDIDALKIQLVNSLRKALVLSQTGSRKYKVVILTPRPGASWFERKLQAYPLQFRNDFQYIIVTGKKSFAPKLKSCLKSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU11</accession>
    <name>A0A1E3NU11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Obg-like ATPase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14922</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020589.1">
        <property type="nucleotide sequence ID" value="XM_019160598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU11"/>
    <dbReference type="STRING" id="763406.A0A1E3NU11"/>
    <dbReference type="GeneID" id="30177285"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF9">
        <property type="entry name" value="OBG-LIKE ATPASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="6">
        <location>
            <begin position="31"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="7">
        <location>
            <begin position="313"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="166"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="405" mass="45256" checksum="58E739F326AF60A7" modified="2017-01-18" version="1">MILQQVRYYAAVSAGASKGSKKLFGRPSNNLTSGIVGLANVGKSTFFQAITKSTLGNPANYPFATIEPEEARVLVPSVELDRLAELYSSKKKIPAILKIFDIAGLVRGASDNKGLGNAFLNDIRSVDGIFQIVRAFKDSEITHIEGSVDPVRDLTIVQDELILKDMEFIENSIEKLEVQLKHKGNKPMHEITSMEQQLEALNAAYDLLMEGQKVINKSDWKDDHIDALNVHNLLTAKPSVILVNVNEEDFLNQRNEFLKDIQTWVDEFSPGSPVILFSADFETKLNEEHQSSMKYNSTSALPAIIQEMRKVLNLISFYTCGDIEARQWTIRQNATCPKAAGVIHTDFEKTFINAELIKFDDVKGLEPPFDEKLLKSKGKIQRVGKNYIVEDGDILHFKAAAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND99</accession>
    <name>A0A1E3ND99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015210.1">
        <property type="nucleotide sequence ID" value="XM_019162058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND99"/>
    <dbReference type="GeneID" id="30178745"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="53" mass="5300" checksum="B7B7A642D82D3CBE" modified="2017-01-18" version="1">MLAATAGHKCCAVLSPIPLVLEPPPFPPLSANDLPAGGIPAAANNGLLLLCLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDD0</accession>
    <name>A0A1E3NDD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Glutamate synthase [NADH]</fullName>
            <ecNumber evidence="8">1.4.1.13</ecNumber>
            <ecNumber evidence="20">1.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PICMEDRAFT_37696</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="26">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13" evidence="8"/>
    <dbReference type="EC" id="1.4.1.14" evidence="20"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015240.1">
        <property type="nucleotide sequence ID" value="XM_019163099.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NDD0"/>
    <dbReference type="GeneID" id="30179786"/>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00045"/>
    <dbReference type="UniPathway" id="UPA00634">
        <property type="reaction ID" value="UER00690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097054">
        <property type="term" value="P:L-glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000031">
        <property type="entry name" value="Glutamate synthase 1 [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000017">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000113">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000022">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.60:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012220">
        <property type="entry name" value="Glu_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000187">
        <property type="entry name" value="GOGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19 26" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0288">FMN</keyword>
    <keyword evidence="18" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="14" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="17 26" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="28">
        <location>
            <begin position="73"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="29">
        <location>
            <begin position="1679"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="984"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="25">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="1208"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="1214"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="1219"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00057049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00068518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="ODQ44127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2148" mass="237594" checksum="CE94450E61E97F57" modified="2017-01-18" version="1">MVVLKSDPAAEYSEFSDDCVSAVSNTSSSSSQPESESSYSVYKYDEVPENKSWASTIPTAKGLYNPEYEKDNCGVGFACQIKGQASHKIISDAKYLLCNMTHRGAVGSDARDGDGAGVMTSIPHKFMQREFDYHCKVQLPPPGQYAVGNLFFKKDDEIFQKSKSTFESIATSLGLRVLGWREVPVDSSILGPASLSREPKTLQPLIVLQEAYGSSNTPLDVSEEEFESKHEKLFEKQLYILRKQSTHTIGLHNWFYICSLSNKNIVYKGQLVPVQVYSYYHDLVNADYECHFALVHSRFSTNTFPSWDRAQPLRWAAHNGEINTLRGNKNWMRAREGVMSSKLFGEDMDKLYPIIEEGGSDSAALDNVLEILVINGVLSLPEAVMLLVPEAWQNNATMDPKKRAFYEWAACLMEPWDGPALFTFADGRFCGANLDRNGLRPCRYYVTDDDIMICASEVGVIPIESNKIVKKGRLHPGRMLLVDTKEGRIVDDRELKKQVANRFDFKAWILSNMITVPELLTKLENRGFNLQSKVDSDVKIKHDPKLLANGYTLEQVLAILVPMVNGGKETLGSMGNDSALACLSQQPRLIYDYFRQLFAQVTNPPIDPIREKIVMSLECYVGPQGNLLEMNPVQCNRLKMTSPILSNDELLALTEINKVYPKWSVTNIDITFDKSEGLTGYTEALDRICAEATQAIIDNSQIIILSDRNLSATKVAVSALIASGAVHHHLVRQKQRSKVAIIVETAEAREVHHFCCLVGYGVDGVNPYLAMDTLIKLNNEKLLTKEFTNREIIDNYKHAIDDGLLKVMSKMGISTLASYKGAQIFEALGVDNSVIDRCFAGTASRIKGITFEYIAQDAFSLHELGYPSRDTIKPKGLPESGEYHWRDGGESHVNEPAAVANIQDAVRNKNGKAYEAYSKKEYDAIKNCTLRGLLDFDFENSSSVPLDQVEPWTEIVRRFCTGAMSYGSISMEAHSTLAVAMNRLGGKSNTGEGGEDAERSKASDNGDSMRSSIKQIASGRFGVTSYYLSDADELQIKMAQGAKPGEGGELPGYKVSESIAKTRHSTPGVGLISPPPHHDIYSIEDLKQLIYDLKCSNPRARVSVKLVSEVGVGIIAAGVAKAKADHILISGHDGGTGAARWTGIKYAGLPWELGLAETHQTLVLNDLRGRVVVQTDGQLKTGRDIAIACLLGAEEWGFATAPLISMGCVYNRKCHTNTCPVGIATQDPVLRKQFKGTPEHVINFFFYVANELRVIMAKLGFRTIDEMVGRAEKLYVRDDLRTTKNANIDLSPILTPAHTIRPGVATKCEKKQDHRLHVRLDNKLIDEAEITLDKGLPVTIEAEAINTDRAIGTTLSYRCSKKFGPTSLPHDTIHVNIRGSAGQSFGAFLASGITLELEGDCNDYVGKGLSGGRLIVYPPKDSKFKAEENIIIGNTCLYGATSGTAFFRGIAAERFAVRNSGAYAVVEGTGDHGCEYMTGGRVIVLGSVGRNFAAGMSGGIAYVLDMAQDFEEKVNRELVELTTLSDPAEIAFVRGLLEDHRHYTNSEIALRILNDFNRFLKKIVKVLPFEYKKVLEAEAKKTAEMKKKEVSNYFSTIKEDPEADITGGEFSKPKKSSKHEPKVLDLEDTMIDVKAEESKATKLDKVGGFIKYSRRHEKYRPVKSRLQDWKELSTRLTKDELKFQTARCIDCGIPFCQSDTGCPISNIIPKWNELVFKDRWYDALQRLLMTNNFPEFTGRICPAPCEGACVVYVSGNAPVGIKSIEAGIIDHAFSEGWIVPNPPKVRTGKKISIIGSGPSGLAAADQLNKAGHTVTVYERADKVGGLLMYGIPNMKLDKGVVQRRVDLMTAEGISFITNTTIGEDIEIDDLKQQNDAVILAIGSTIPRDLKIPGRELKNIDYAMTLLTANTKALLENDLETIRATITGKNVIVIGGGDTGNDCLGTATRHGAKSVVNFELLPHPPAERARDNPWPQWPRIFRVDYGHTEVKEHYGKDPREYCILSKEFVDDGEGNVKGIKTVRVEWKRSDSGAWQMQEIPGSEEFFPADIVLLSMGFIGPESDNLNIERTKRGTIATFEPSSYQTSSGSNLFTAGDCRRGQSLVVWGIQEGRQCAREVDIYLMGKTRLPGNGSFEKRDYRLLEELASKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDH4</accession>
    <name>A0A1E3NDH4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015269.1">
        <property type="nucleotide sequence ID" value="XM_019164862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDH4"/>
    <dbReference type="GeneID" id="30181549"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="91" mass="10682" checksum="F6F16E7DB67A012B" modified="2017-01-18" version="1">MDQKIRMSSVRIRMSSVRIRMSSVRIKMSSVRIKMSSVRIKMSSVKRYEVSRWLAVIDEMDQKIRMSSVRIRMSSVKSVICDIPDEPMARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDR4</accession>
    <name>A0A1E3NDR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18498</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015367.1">
        <property type="nucleotide sequence ID" value="XM_019162029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDR4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDR4"/>
    <dbReference type="GeneID" id="30178716"/>
    <dbReference type="OrthoDB" id="2110451at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024789">
        <property type="entry name" value="APC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="7">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="8">
        <location>
            <begin position="310"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="275"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="656" mass="74695" checksum="05991C60011A6386" modified="2017-01-18" version="1">MDLVSFQLNDNVVWVYRRGNEKVWDIEHAETTPDEANGNGDGGNTKKIKDLAWKPDGKCFAVVSTDGLIELFDTMSGVLIRSIHLEMEISCVKWFKRNPEVNNGLNRSRVGGILDDVAITNSLPSLKFKMNNGTSGTSKEEDNDSVLDFLIAGSTGGSLSCVFSGIFVVENLTLCDIFENSEIMDIISNKSLSNHYVLLNTPASRDISVLKVDTDFIHRDKKFTKILLICSKLLNLIDHIKSSIHQIDSHYKPYFDYTIRIVELLRREIKEDEIENDNKSGNKNTDSAGRGDNDENDENDPNPPTKEDINNNSDPIYDLYDLLLTGSLSNATKKWLTDYLSDRGVKRWTKLGRTYFDNARSSIYNDLVSCLHHMIVFLTDLKGLSQWASNDTSLYTLDIEECIKISQSYLKYSYKFMMELNDSQRYFEQTIIWLSSILSELTADEKLNVSFRTNDITKFLMFVSSKINAVDSDSDHGSIHDASKVNEFTKYLDTVLNSLFGKIKNDIKSKFKLDTHSLLFSNENSSVSHVSMKIFSDDNKGHALMLLDSSQIVVKEFNLTTLDVQTYEMTREGTETITDMKIVSSDRLLVLFDDCVIMYKLLLDTKEAVADFKYVLQEHSNETDSDFQAGYLAVNEDKKLFCVLDKTKKKYVWISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDW1</accession>
    <name>A0A1E3NDW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_37288</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015423.1">
        <property type="nucleotide sequence ID" value="XM_019163073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDW1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDW1"/>
    <dbReference type="GeneID" id="30179760"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF162">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="3">
        <location>
            <begin position="14"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="348" mass="39402" checksum="2D76B032F6BE3B3D" modified="2017-01-18" version="1">MEETKLTQDEISLYDRQIRLWGMEAQTNLRNSNILVINLTGVGVEIVKNLTLGGVGSLTLMDSSTLKEQELNSNFFVDKSQIGMLKVDASKNRIQDMNPRVQFKSDSRDWEDLGEEEFCKYQLIVCTGLNSSQVSKLNKISRKISIPLIACCVHGMYGLIFNDLIKCQSWIKLEKSALREVGDIDMVSKILALEEVTENDVELQKVLVENEYRSWDELSGRFLDAQLPTDKKKKKRVNPALVSLLALLELPGTYLNKDIEDVVIERDLLDAKIGKVLEKYNLPSSIQMDDTSLERFIKNAYCEYQPTNAIVGGVVSQDIINMLVHKEVPINNVSVIDGFNCEMPVYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDY1</accession>
    <name>A0A1E3NDY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_13539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015445.1">
        <property type="nucleotide sequence ID" value="XM_019160075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDY1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDY1"/>
    <dbReference type="GeneID" id="30176762"/>
    <dbReference type="OrthoDB" id="4088889at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035189">
        <property type="entry name" value="Std1/Mth1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17235">
        <property type="entry name" value="STD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="387" mass="44811" checksum="41CCE624D20C2E4C" modified="2017-01-18" version="1">MNASEDGTRNSLNHRPKIKSVNIPYYFQGEMEDSISLQIKSLVYVFHRFQISNLKPFLKISDLTDEYDFDYLGSNHHSNFNGANKSANQRSSMNSRSILMNDKLDRIYNVDHFKTQLLNDPYRILIDYSKNKFRSLLILSEAPSEFSEIPSLNTPNFNFLILKPDDPTEKYVEVIVNKSGIYSEHRINNKIKVQILLRIINRQKKALESNNANSPEFSGEFTKDDRANIIKSYVQKLAFYIQVQRIYKAALKSKERDMKNQLYKYSNDKSIQSYKSLKSERSRMILLPESTTSPIKNTNMNANNSSTGLLPNSTNHQISIMDLDDTVNQNNSDYPYYSDTADSQASSSTSSVNFVKLFTPEEKHVCFEQSKMAVRIRIERERTLLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE57</accession>
    <name>A0A1E3NE57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74662</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015534.1">
        <property type="nucleotide sequence ID" value="XM_019164836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE57"/>
    <dbReference type="STRING" id="763406.A0A1E3NE57"/>
    <dbReference type="GeneID" id="30181523"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="325"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="245"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="341"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="428" mass="46432" checksum="6A312C5752C00CFF" modified="2017-01-18" version="1">MNPSSSFTFENFHLDPEDFQNFLLINPPLTEYSSHYYMNLSNSGTASNANANAHGVAASAGATGGHLGHGGHGGRSLGTNAITDSVPATNTPMRSLSGIPDSDQLLNQLSLKKQIPVSVAPLTEQLSSVAPTSQMTFPPFTGDDNDAIRNAAAGATLLPTNNTNTNTNTANATAASLSGISFPSNAHALASQSTQAAQQSAALFSNQYAFDADVRFDDQQLPLPTRRLSISNGQIGQISRMVHSQMYPSSTSNSSGTPNSNDSTAVSNHSSGSARQPQKAQQKALLDEEESIEVDTNGVPTRELLYNNEVIFNPNGPIPGTNAWKRAKILERNRIAASKCRAKKKNLQKKLQQDVDKLSEENSRLRDMMTEMKKRVERYCQKNNIDIAEVYASDSQLKKEQQEEEMLKKKKAQNTSELVDTFLKENVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE64</accession>
    <name>A0A1E3NE64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CinA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015499.1">
        <property type="nucleotide sequence ID" value="XM_019161939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE64"/>
    <dbReference type="STRING" id="763406.A0A1E3NE64"/>
    <dbReference type="GeneID" id="30178626"/>
    <dbReference type="OrthoDB" id="2350783at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="entry name" value="CinA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="CinA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA C-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="182" mass="19800" checksum="536B2F090BD99BC7" modified="2017-01-18" version="1">MNFPPEDIKQLVAEIAEILIRKNQTVSVSEGACGGLLSAYLISLPGASSFFDGGRSIYSLKSRLRLSGWNNQQINSYTGPCENVALKMARNLRVEFGSTYVLSETGYAGPSTNVGLGHDNGSINDADAKVGTVYLGICGPNGGHSCEMCTESNDRSTNMQLFAKYSLQFFLRILKQEQEKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE77</accession>
    <name>A0A1E3NE77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DMAP1-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18348</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015560.1">
        <property type="nucleotide sequence ID" value="XM_019161960.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NE77"/>
    <dbReference type="GeneID" id="30178647"/>
    <dbReference type="OrthoDB" id="69964at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056881">
        <property type="entry name" value="Mug62_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF32">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23024">
        <property type="entry name" value="AMP-dom_DIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24919">
        <property type="entry name" value="Mug62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DMAP1-binding" evidence="3">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="153"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Meiotically up-regulated gene 62 protein-like alpha-beta" evidence="5">
        <location>
            <begin position="665"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="1404"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1535" mass="173133" checksum="DF5533791355CBBD" modified="2017-01-18" version="1">MDFTVPKDVSPELDLLLTRLAKDFHDGYLTEKGYIKKRMELLGVMVSATSENHTEPNTEGLQTVSTRQSASISDRASEDFFRPSHTISHSVDESSNAQPVLYDDEFLEKYHNLGSTYDSNIRRELQKPLDPRDVSSHIENANEFDNLAMILRKRAKIYEKEPAVLVVDEKGKESKVITWDKLYYRAEKIAKQIKNKVALYPGDRVCLIYQNVEIIDFMVAFYGCFLAGVVAVPLASSMPSKELATVMRNTQSHLCLMSDSVHKYFEKQSKQTKSVLWPKGMIVWKTSALGTYKPSKKDGDPPLKISDLAYIDYSKGSSGELRGVVMSHRTVINQMKMLDKILSTSPNIVSPLTRSTLKSTRPKNSILSTLDVRGSIGLIFSMLFTVYSGNLFIWLQSKTAEVPGLYANMLSCYRSSITLTDYFTLKNVVYNYQSFPQNTRTFSKKKVDLSPLCWCLIDSSTVDCEFNEILSDRWLKPLGSKHSKRIISPILTLSEHGGAIISMRDWYGNEDQLGCIFNKSENSETLDEFGDDDHADRLSEVMIDKESLTTNSVKVINDAPINYSLAESNSDSNYIRVGAFGYPLPDATLALVNPESKVLSSLLEVGEIWVDSNCISGGYWGLPDETEAIFQAECSDYEGILSLKFVRTELLGFIYNGKIYVLGLYEDRINQRVTWYDQYLGMSTDDNNNQLLPVINDRTKSMTSDAESIYKTMMKKKYNNWGRLDLSKNLSNKSLYKYHYSNHLAKTLAKNVPYFKDCAFFNMKVNNEYVPVGIMESGLSTDKSVSGKVELQSMLTEALNLLEKHHNVRLFFLVVTKVGALSRTLKSGRLEIANSLTKRRFLEGRLPSVYVSFKPYNSLGSIYHGEDLVGGIWSPYSTAVRNDMLSYSDFQSSGMDTRDSSVDDRFHINLTDFANPILLLQYRASKQSDELAYTSIDGTNAKENKQSTWKKFESRVFTVCSYILEKSTIMSGDCVILLYPLCEDYIACLFACWMAGFTVIPLPTFAKDPQSIDEDVETFVNAVKEYNVKAIFVNNETENLLKNKPVSSKISALASKINLEIPKLRNTVKHSKTLSAGKSTYKRMEQYSEKKRSRKNSDYCMIWLDLTPDNVCTSVGLSFKNLMNMCLSLKETCQMSSTAPLLGCANYTVGLGFLQSSIMGVYLGVTTYLMSMAEYGLHTSMFYQAIARYNIENIYLTGKMFKYALNKNSMGKINLKTLRNLMLSWSGCRADPSLLSKFKETLVLSNVKSKTISHIYDHKMNPMISTRSFLSFAPVVLWLDPYGLSQGYISIVNPKDAPNAIPVVDSGVVSVNTEVVVVNPQTEELCKVGEFGEIWVSSKSTASGFAGKDDNSTNKEYLKGKLNNRNGTFLRTGDFGFLHTITKNTDKNKQIEMQLLFNLGKIDETFDYCGLQFFASDIERCMDSFAGMNRCCVFKAGDYTVLVAATSTGRSLSTVTPLMIVKLLNQFKIVIDIISFVDSNGLPTSRDGMIQRMQLVRKWLNKTLKVNETFGISYGENEMIKAIKMQEWVEDRLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEI4</accession>
    <name>A0A1E3NEI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015670.1">
        <property type="nucleotide sequence ID" value="XM_019164728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEI4"/>
    <dbReference type="GeneID" id="30181415"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="163" mass="18511" checksum="273C12D05DEADE6A" modified="2017-01-18" version="1">MPAEQTLNLDKYPTDTKLLLCQLVYDAKEGNTWEDILDRFINHPIITRLHGTSIMEFANIKADDLSTLVIGIINDTVSKVKAADTSGTTVKQPRGRRSQRNSHDEKLIEESVVGLEFFQLCDHQGRELLILCCGKLHKLCVAEIKRKLSNNRKEFSQLMSQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEK3</accession>
    <name>A0A1E3NEK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor spt8 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_36608</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015690.1">
        <property type="nucleotide sequence ID" value="XM_019163020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEK3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEK3"/>
    <dbReference type="GeneID" id="30179707"/>
    <dbReference type="OrthoDB" id="10260946at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057544">
        <property type="entry name" value="Beta-prop_SPT8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23798">
        <property type="entry name" value="Beta-prop_SPT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="3">
        <location>
            <begin position="19"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="159"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="495" mass="55209" checksum="3A0C55439A8339AD" modified="2017-01-18" version="1">MERKKLIEESVTREIAGVNPVVAIPYATSVYSISLTRGPKWLFTGGEDGLIRKFDFFKSVEGKSLLTVSQRHQLIDSILFNGMICSYWENEIPYYKSDLSGNKAGSPVYSLCSDQNGFWLLSGLQNGAITLQSVRSSEGTIQWYFKNANDKTVSSEDKQYHHSNSVSCIVTNTEQTNFLSGSWDRKILKWDLNTGKNMNCFNRSTGQVSALQYRPSIGTDLHWEATNDDDDDDDNNNNNDDDDMGSLFDDDDDNDGANEDRRDKSDAAKKEAADEPGLEPKEKAASQEPEVRRDILKLQRESLQTGTYEGQRVSDDIFLSSSIDGSINIWDARIAPRANNVEKIPVPASTPPWCMSATWSVDGDSVFVGRRNSTVEQYDVRFPTKVSSLLKFPSASGSISCVRTLPNRNYLLCGCQDNIRLYDLRLHDAPATAPTASAPSSRKVSRIPFTIVPGHNGGILSDLYVDPTCRFMVSASGNRGWQGKPSDYVFIYEFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEQ0</accession>
    <name>A0A1E3NEQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycosyltransferase family 62 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18016</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015717.1">
        <property type="nucleotide sequence ID" value="XM_019161851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NEQ0"/>
    <dbReference type="GeneID" id="30178538"/>
    <dbReference type="OrthoDB" id="204164at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF2">
        <property type="entry name" value="MANNAN POLYMERASE II COMPLEX ANP1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="50111" checksum="9910C2CB54CA7E57" modified="2017-01-18" version="1">MPPEGLILKHTSFSLKSAFSIILTFFACSYLLYSWKGYLELKGNSLGDPTERERELYRPVLTEFPHEEVSYIDLKQDVQATAYGWQNNERVLFLVPLRDAAAHLPMFFSHMSNMTYPHGLIDLAFLVSDSKDKTLEVLNESLKAIQESSNPDMPFGEIEIFEKDFGQAVGQGFSDRHGFEAQGPRRKNMAKARNWLTAVSLKPHHSWVYWRDVDVETIPPTIIEDLMSHDKDVIVPNVWRPLPDWLGQQQPYDLNSWQESDGGLQLAESLDEDAVIVEGYPAFATWRPHLAYLRDPYGDPRTELQLDGIGGVSILAKASVFRKGAHFPAFSFEKHAETEGLGKMCKKMGLEVVGLPHYVIWHIYEPSDDDLRHMEWMAQEEVRKEKEGEVREIYDKNFKVGFELIGDVWENEKKRILKNSDPFELQRPVFVDWSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NES4</accession>
    <name>A0A1E3NES4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 7 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_74336</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015732.1">
        <property type="nucleotide sequence ID" value="XM_019164738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NES4"/>
    <dbReference type="STRING" id="763406.A0A1E3NES4"/>
    <dbReference type="GeneID" id="30181425"/>
    <dbReference type="OrthoDB" id="5390at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040447">
        <property type="entry name" value="RRM_Rrp7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040446">
        <property type="entry name" value="RRP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024326">
        <property type="entry name" value="RRP7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 7 HOMOLOG A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17799">
        <property type="entry name" value="RRM_Rrp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12923">
        <property type="entry name" value="RRP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rrp7 RRM-like N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 7 C-terminal" evidence="3">
        <location>
            <begin position="177"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="34413" checksum="F4BCAE7F574112C7" modified="2017-01-18" version="1">MPVDLTPIKGFLPLPIAIPAVANLPPSTHLCYIKPHMSKDPSEQADTTKSLFLINPLPLWTLDNVKKLFRQVNNASHIEKILIREAIDTSRVSSNGSGVNYDLHINLSKLTNEDYGCELEESERLPFGSSVITFLDRDGLELFLSSVKKIKKALEWDVTNSSSETGLQRYTRIPYVIDRKVAEKEVAKTLIDFQQREKKAEVEVQNMREIVDEDGFTLVVGSQKKTKSDILGSMKKLSDLEKDEAHVKKNKKKEKKDFYRFQIRERKKQEMNQLLSKFKEDQERVKQMRQKRRFRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEZ5</accession>
    <name>A0A1E3NEZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-oxoprolinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18098</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015813.1">
        <property type="nucleotide sequence ID" value="XM_019161877.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NEZ5"/>
    <dbReference type="GeneID" id="30178564"/>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="2">
        <location>
            <begin position="239"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="5">
        <location>
            <begin position="678"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="3">
        <location>
            <begin position="750"/>
            <end position="1283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1317" mass="143589" checksum="E049DDF8D1F835EE" modified="2017-01-18" version="1">MSSIQIAIDRGGTFTDCIGNPGTGRQEDDVLIKLLSVDPQNYSDSNLEGIRRLLEKLTGEKQDRGSSLDTSRVAYLRLGTTVATNALLERKGSRCALVTTKGFRDILNIGNQSRPKIFDLSCAKSDTLFETVVEVDERVTLEDYTEDANKGFTKPNGKDLVEGLSSETVRIIKAPDVEEIKSTLRVLYASGIRSIAVCLMHSYTFPDHEKIVGNIAKEIGFTHISLSSVLSPMIKYVTRANSAVVDAYLTPEIKKYLASFQEGLMNGLYSAENPNGVKCHFMQSDGGLVDAGCFTGLKSILSGPAGGVVGYSKTCFNTDNNIPLIGFDMGGTSTDVSRFGEGRLDHVFETTTAGVTIQSPQLDINTVAAGGGSILSFKNGLMHVGPGSASSHPGPACYRKGGPLTVTDANLILGRLLPEYFPKIFGPNEDEGLDIDITEKKFKELTEEINLAHPDSIPMSIYQVAQGFLEVANETMAGPIRQLTESRGHSLNKHRLVSFGGAGGQSACAISQALGIDTVLIHRYSSVLSAYGMALADVVEDIQEPASYVLNTDNKKVFMQKFDHLKNRAFVSLSNQGFDENNVEYELYLNMRYIGTESSIMVRKGNSWDFGDHFRELHEKEFGFSFEKDIAVDNIRVRAIGKTNIRVQENIDDQINALENDNLIRTVGKGKVEFLKMCFWDSQFQETKVFRLEKLDIGTKVDGPSIIADDTQTNVVPPHCHALILKSHIVVKIQKKNGHGNTKPINNGINPVMLSVFGHRFTDIADQMGVSLQKTSVSVNVKERLDFSCALFDSNGNLVANAPHVPVHLGSMSTCIKSQLKIHKGKLKEGDVLITNHPSTGGTHLPDITIITPVFQDGKMLFVTASRAHHADIGGILPGSMPPSSTELWQEGAAFYSELLVTEGEFQIDMLKKILVDIPAKYPGCSGTRRLSDNISDVKAQIAANRKGIALIRALIAEFGVETLLMYMKAIQDNAASTVARMLEKIVNSHDVLSCRDYMDDGSYIDLEVYRDPDDNKVVFDFANSSPQSYNNLNAPVAITYSAIIYVLRCMVNEDIPLNQGCLEPIKVKVAERSILSPSEFCAVVGGNVCTSQRITDTILKAFNLMADSQGCCNNFTFGKGGKNEVGENNEGFGYYETIAGGHGASSTWNGVSGVHTNMTNTRITDAEVFEKRYPVMLAEFSINAGTGGNGEFKGGDGVVREVEFREKVCASILSERRVIAPHGVNGGEDGRRGLNLWIRNIKDSDGNVVGKNVVNIGGRNSVNVDVGDRIRIETPGGGGFGKRRPDGLKINSCMSKPLNMLHTGSLVGRRKLQYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFD0</accession>
    <name>A0A1E3NFD0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_26884</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Is probably involved in a pathway contributing to genomic integrity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IRC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015942.1">
        <property type="nucleotide sequence ID" value="XM_019162468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFD0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFD0"/>
    <dbReference type="GeneID" id="30179155"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="172" mass="18861" checksum="F241A0181BD4247B" modified="2017-01-18" version="1" fragment="single">TDPNFVPSPEKPFNFLIDYEISFKEDKEAGVIGDLYNGETIELAYNFRSLEPSEVSIVGVGGELLDPVTGISLANITASQIGPIAVENNQLVNFTQRVGINMEPRKYLLVPAIYIVYQDQFMLLGSTNKLVNIVDPKISLFNPQLLVAELILGATVAGIVYLAYNAFAGKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFL1</accession>
    <name>A0A1E3NFL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_156389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016034.1">
        <property type="nucleotide sequence ID" value="XM_019160902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFL1"/>
    <dbReference type="GeneID" id="30177589"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="93" mass="9731" checksum="4D08726E4A449C08" modified="2017-01-18" version="1">MGYANESSGGDLPGEGATKIGPRQTPNDDTKWSSGGCRRRISPGASPGFVPCLRVCWGCKNRTSWLRNRLEADRTAGAAEALAEKGHGADLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFL5</accession>
    <name>A0A1E3NFL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_26003</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015246.1">
        <property type="nucleotide sequence ID" value="XM_019162426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFL5"/>
    <dbReference type="STRING" id="763406.A0A1E3NFL5"/>
    <dbReference type="GeneID" id="30179113"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="15"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="457" mass="50469" checksum="3D08F2518B7BBAE8" modified="2017-01-18" version="1" fragment="single">IRNVVPLEKRNVFVVYTLILLATSLDNMNTSAAFTMSNDIEKAFNTDSSTASWVLSGYALTLGSFILITGKLADVIGPHNIFLAGLGIIWICSLICACIPHTSIVALIVFRALKGIGASALVPSTVAIAANYFTGDYAKYLSPALAGFLIALTGVFGVGVTLGGAFSETNVGYRGFFWFVFAYAFVIDLTLLFIIIPVDKTEGHKRMKLKNIDFFGGFLVITGVLLIILGLTEGAENWRSPKAYVPLIVGVFTFLSSLGFETIYLKRFQRKNQNLDKSADWRLQVELLFPPEIIKIPNFLPILIQCGMYYATFSMVMVIGINYYTYIEKESPIIVAMKVLPLPVGMVFGALVYRASYYNRVGLKNMLILSSLLTLGMAIWFSRITYKTSHSYWKYGFISLFIYGYGINMFFNIYYSVVVSNTPLHLQGVVNGIFQTCGQVLLAIGNALVPSILGNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFT3</accession>
    <name>A0A1E3NFT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_36159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in endocytosis and clathrin cage assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with PAN1 and the clathrin heavy and light chains CHC1 and CLC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AP180 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016097.1">
        <property type="nucleotide sequence ID" value="XM_019162990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFT3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFT3"/>
    <dbReference type="GeneID" id="30179677"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16988">
        <property type="entry name" value="ANTH_N_YAP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000004">
        <property type="entry name" value="ENTH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="6">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00058079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="626" mass="69962" checksum="37EE7095FA717F4E" modified="2017-01-18" version="1">MTTFDKLVDGATKIKLAPPKPKYIEPILLATTEGERSDDFRTVMRALGRRLQDSAWTIVYKSLLVIHIMIREGDENVCIKYLSSHLSVLDIHIGKSGKFISNGGELNQVYSYAHYLATRAKEFANTGHDFIRETKKPYGSWNSSDKSSILREMPVEKGLLRETESVQRQIDALVRCKFRESEINNDLLVLSFRMLTTDLISLYQCLNEGVLNLLEHFFDLSKSDAERAFEIYEHFTKETKKAIEFLRVAKHLESVTKLRVPTIKHAQTSLTSSLREYLDDPDFEINRRQYLAEKEFSKQGGTQQQALQQQALQQQAHAQAQAQQLQFQMLQAQATANAGVQQQATGFNPFSNMNTFSMQPAQATVPQPQPLTIQTSQSYIQNPMAQMNLAATVPMNLQTPVQQTPNAFSTDSSLVQPRVLSNNFTGNGFGASTSTTTPLTTQADQELQRRLQNEAHTGGSNAVRAVATGTNPFRLETHSTASPSHSSNGGLATVSELKMQPTAGGLERLPTVPVFPETQRDAYQQQLQRQAGIELSQQVLQKQMTSNPFSQQATGMIQQASLNPNSYTGNNGNDFNFVGMSASNVPNAYLIQQQQLQQQMLQQQQQQQQQQQPVYSSIYNGPNLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFT9</accession>
    <name>A0A1E3NFT9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_13764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015326.1">
        <property type="nucleotide sequence ID" value="XM_019160108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFT9"/>
    <dbReference type="GeneID" id="30176795"/>
    <dbReference type="OrthoDB" id="3997502at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="228" mass="25339" checksum="4F4CD66BE2616A0D" modified="2017-01-18" version="1">MLKRTVRCLAPAGGDSTTPVKLLGFVPGRLTRATKKKKTFREMNKSPAPDEELTRFLKTSLNAYPINVVVRNKARALSYHLKRLVSDIEEIQDTAPDEDPNDLPLLIDYKVIDATRNKPLHRAFCRQYPQMQGVEDSSIVVVSFTKEGLDTDTSFISSFQKKYEDAFGVSGCIVNTHTYPYLRDLSRSKNGGVGENMYNIAALGLVKVHKQDDGSLVLTRGGSRGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG63</accession>
    <name>A0A1E3NG63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_36426</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016222.1">
        <property type="nucleotide sequence ID" value="XM_019163004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG63"/>
    <dbReference type="STRING" id="763406.A0A1E3NG63"/>
    <dbReference type="GeneID" id="30179691"/>
    <dbReference type="OrthoDB" id="277802at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="129" mass="14831" checksum="9317B9F10B7882C8" modified="2017-01-18" version="1">MPPRKRAYPGTGKPGKATKSKENAKRQHTEASHTLYLSNLNTKIKPHRMKENLYVLFTSFADVLEINYPRKNFRGQGWIVVTSPDDANECLAKLEGFDFFDQPLHVRYARKDSNIIHSLKKLEDATLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGM9</accession>
    <name>A0A1E3NGM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar import/degradation Vid27 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016387.1">
        <property type="nucleotide sequence ID" value="XM_019161727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGM9"/>
    <dbReference type="GeneID" id="30178414"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040979">
        <property type="entry name" value="Vid27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040768">
        <property type="entry name" value="Vid27_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17748">
        <property type="entry name" value="VID27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17747">
        <property type="entry name" value="VID27_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vid27 N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Vid27 PH-like" evidence="3">
        <location>
            <begin position="287"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="472"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="831" mass="95079" checksum="9D90D8700049C603" modified="2017-01-18" version="1">MNIVKRFFMNHNKYPTDESSMIPSGQLYLLRSPSSPKSENECLYNDAILTVRETNHQFDYQLVVWKGNPSDSESSSDDDNGDSDVNDNFFENESNVLKSFLIDSSLNLCLFERYSQKIISWRDMEGDLGDMFEYRITSTVPNDTIEQFMTAIYKCKYERKYQKSSANLSLKDVQEFIVDRNQLFDNCLNSANTPSISSNLNMDELLTPKRNIEDEVEFAEEGESDDEEDELEERVEHRNLSYNMNATSYEDEEEDENNFVDASESPQTAIFSVQKTNRVLIRDFPCKVYSYSAEAQAFESVSNQSLVLLYELSNWNYCFEVREEDNSTIYAVSLINDQLDPTFKFEQFAFLFNLYTKTSATTWLLKFEDRTTYEGFQATYMKLHWQNRNETTWPTRKEDEQYLIDSLDMMDIDDDLSQADEETEDSDSEQPEVEDITGTSSFKFKGRKKYVDLDDSDESDDENEREFNKVSKNSGLVLGLSTDRAFISRANKIGIFKTTDSGIEFTAAINKLSHSRNAKKELIPSKMMLMDNDRTMIVQDKSAMNSLHKVDLEYGRVVEDWKLEKDDKDVNVENFTTNRKFGDLAADMTFLGVSSQSLFRVDPRMSAGFVSDDAIKAYKGKTNFKQVSTTGGGYIAVASKNGEIRLYDQLGKNAKTLLPALGDEIIGLDISANGRWLLATCASYILLIDVQIKDGKFEGKLGFERSFSVKDKPMPRRLNLKPEHMAYVKQRYGLQTSFSIAAFNETSEGGSPTYIVSSIGPFAVTWNFLKVISNVRDCYKIRMYQENVVISEFVNHNNNHMVLALPDNVALASTSSFKRPSDEFSVVKTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGY8</accession>
    <name>A0A1E3NGY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ammonia transport outward protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74147</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016497.1">
        <property type="nucleotide sequence ID" value="XM_019164687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGY8"/>
    <dbReference type="STRING" id="763406.A0A1E3NGY8"/>
    <dbReference type="GeneID" id="30181374"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF3">
        <property type="entry name" value="AMMONIA TRANSPORT OUTWARD PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="30653" checksum="6E41F4799A6B0C00" modified="2017-01-18" version="1">MNNNSFNDDLEKQANDDSAGSNEGFPRVDHQLTHKSFRHDDDHIYINDVPINKAEFTQAFGGTFEVSRREKTRELREYGNPVPAGLGAFSASAISLGLVQMHAKHVHQANTLLGAFLTTSGLVEIIVGILCFVIGNTWASCTFLMFGGFWSSYAFVLMDVGGIAASYSSAAEYGQSVAIFFLPWAMFSFFLWICTWKSTVALSLLMFLIWFFVLLFTIAQFISSVNLFKAGGFFAIFAGCVGFYNMLAGLADKTNTYFIIKPVLLPNQPTVEELKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHA2</accession>
    <name>A0A1E3NHA2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73739</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GcvT family. CAF17/IBA57 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016051.1">
        <property type="nucleotide sequence ID" value="XM_019164570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHA2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHA2"/>
    <dbReference type="GeneID" id="30181257"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602:SF0">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="372"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00093447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="463" mass="51819" checksum="5C82EA63CE734FD2" modified="2017-01-18" version="1">MFALWKRGNAAVFVTLAKRSLSVPARIPGRGLVKLDSSRFLRVSGPDASVFINGLTTVKMLPKHLKKNQTTISAADINNEAVARSIDLSQELITSSNWGILHEAEEYDPEDPAELPMRLGIRRDGRYGMILRANGRVFSDIFIYPTPFVLDGDAASEPTYLIELLNAAQFKPLQLMLKLHKLRAKVDIQEEKLASWFYYDNSPDGHKLYDTLLDVYFSNSASKDPAAANQLAARLLSDGGLISPRLPRKDVLGFAVDQRSDYFGLRIVSPEEQPPIDNVDLLPLETYVARRVQHGIVENTDFQSTATLPFECNLDWMRGINYDKGCYMGQELTIRTWTGNGTVRRVLPVVFDEKIPDLDDAHAKLELRVVEDSAEAQQEQKEEKEPVYNPFGSPASSSAGARPVRARRDAGKVGEVLVNDGERGLARVEKRYFDWDQELTKKVSIVHNGHTYAGTIDAGIWNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHB1</accession>
    <name>A0A1E3NHB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_27578</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016627.1">
        <property type="nucleotide sequence ID" value="XM_019162503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHB1"/>
    <dbReference type="GeneID" id="30179190"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001485">
        <property type="entry name" value="Krueppel-related zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000130">
        <property type="entry name" value="Spalt-like transcription factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002157">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="295" mass="33799" checksum="3E51559E60669D49" modified="2017-01-18" version="1" fragment="single">QQQQQQQRQQHRQQTPQQHERYEKQQHPQDYNSNSNNSNNNHNNNISSSNTNNTKEKKLINSNSSLKRDDGRPLVGATKVDQLMLIIQARQKGVCGNIEQSEDGNIDEENILPNPIELVGGVEKTSLKGDKSHQCKYCQKKFTQSTHLDVHLRSHMGVKPYKCEFCDKRFTQGGNLRTHMRLHTGEKPFKCNICDKQFSRKGNLQAHHLTHDNSKPFICKFDGCNKGFTQLGNLKAHQNRFHLDTINELFNKLAQLGEGIGSYDSLPKSEKDLLDYFSSLYKNLNKGIKGRGKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHP9</accession>
    <name>A0A1E3NHP9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73176</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016775.1">
        <property type="nucleotide sequence ID" value="XM_019164404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHP9"/>
    <dbReference type="GeneID" id="30181091"/>
    <dbReference type="OrthoDB" id="4023481at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008401">
        <property type="entry name" value="Apc13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05839">
        <property type="entry name" value="Apc13p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="243" mass="27926" checksum="A5025E9431E27124" modified="2017-01-18" version="1">MVSDSTFSYVHMDSQVSVLFVEAWENQKLRWDDVEFPNGEAVEQHDNLLQQFLSQNEETDTDLTVMNSSNPMFNASYANRVKQKSRKLNWKDMDLNAFSSKNNKKNQIHDLTILNKPNLGRYPSNNDMDTFLGLAERAELGISDNGINEDTIANDRIIRQFSMNSDEELDFDNDSAVTNRKLSNPDVSRSFSYQYATPTLNNKSSIHNFQGSSHRTLSQQKRQVSDNSTRNISYNTPGHRNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHU3</accession>
    <name>A0A1E3NHU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_12158</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016812.1">
        <property type="nucleotide sequence ID" value="XM_019159891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHU3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHU3"/>
    <dbReference type="GeneID" id="30176578"/>
    <dbReference type="OrthoDB" id="204949at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000003">
        <property type="entry name" value="Proteasome subunit beta type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="205" mass="22602" checksum="6E48D24A0995FFA2" modified="2017-01-18" version="1">MSDPSSINGGTAVAMVGKDCIAIASDMRLGSQSLGVSNNFEKIFNYDHVFFGLTGLASDVITVNENFRTKTNLYKMREERNIEPEKFANLVSSSLYERRFGPWFVGPIVAGLNSKTGKPFICGFDSIGCIDFAKDFVVSGTASDQLFGMCETLYEPNLEPEDLFECISQALLNAADRDALSGWGAVVYIVTKDKVVKRYLKSRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHV5</accession>
    <name>A0A1E3NHV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rhomboid-type serine protease 2</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Rhomboid protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_73718</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable rhomboid-type serine protease that catalyzes intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">cis-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016026.1">
        <property type="nucleotide sequence ID" value="XM_019164563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHV5"/>
    <dbReference type="STRING" id="763406.A0A1E3NHV5"/>
    <dbReference type="GeneID" id="30181250"/>
    <dbReference type="OrthoDB" id="10257275at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013950">
        <property type="entry name" value="Mis14/Nsl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF1">
        <property type="entry name" value="RHOMBOID PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08641">
        <property type="entry name" value="Mis14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="15">
        <location>
            <begin position="126"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="370"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ44913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="405" mass="44299" checksum="CECAB86A4D396569" modified="2017-01-18" version="1">MQSRLAERLAQLADEDTETETEDRRELERLLETNVEGRFLDRLVQELCGACVVDEDGGGDEHEGGHAEARKVEPYDFALNDRLRTTYQHFEDLVQDTCQLRRDFVQSWALSPNSLIVEHKGVLLNAYPLVHESLVHLLFNVMVLHHTLSEFEVSHGSLHTAIILNTLGVVTGIAYTITEYILVQLGLANAAILDTAILGSSGWVFAFITAHCCLKSITSPTTNIYASYNIPTLLVPLFYLAVSAVLVPNSSFLGHLISIILGVLIFKDFFSLITIPPFQALDRIERLAIFKWGIRTFFPADYFVWSWEHEVKSSRYLESNFHSVSLPLHHGIGPGASTATGTDPVTGTGSGTVAESVLGSLANASSSNVNITDSVSNTNTNSNSANAKAATAHFEGPGEKLGSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHY8</accession>
    <name>A0A1E3NHY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_19642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016865.1">
        <property type="nucleotide sequence ID" value="XM_019162131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHY8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHY8"/>
    <dbReference type="GeneID" id="30178818"/>
    <dbReference type="OrthoDB" id="10041966at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050262">
        <property type="term" value="F:ribosylnicotinamide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046495">
        <property type="term" value="P:nicotinamide riboside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02024">
        <property type="entry name" value="NRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="193" mass="22509" checksum="5B80609D22B45B99" modified="2017-01-18" version="1" fragment="single">RTILIGITGASSSGKSTLAHLLKSILPHSEIIHEDDFYKPEEDIPYDPIRKDRDWDCPDAIDMDAMKQTLSVLTKPNTTEPTVNDAFFNNDERIIKDLMEKVNVKLEHFDSRESFRIFLIDGFLILPDKELLKMFSLTLFFKTSYEALKNRREKRTYNVGGDVWADPPGYFDTFVWPGYYTYHKNLFIRGNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI65</accession>
    <name>A0A1E3NI65_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_160624</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016146.1">
        <property type="nucleotide sequence ID" value="XM_019161077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI65"/>
    <dbReference type="GeneID" id="30177764"/>
    <dbReference type="OrthoDB" id="3996163at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133368" description="PA14 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="3">
        <location>
            <begin position="55"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="3">
        <location>
            <begin position="325"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="543" mass="58971" checksum="2368F26E245A1040" modified="2017-01-18" version="1">MFSNVFLAATLLLSLVNTTIADRNVWSKSVDGVGALMQLNTDVPKTTGFAATFYSYPWGDFIPFWKDSWVQESYSFNTIRATANAVTSPNFSLNGGNQNLYGLYNINMENVVIELKGYYSPTASGVHRVTANEINDGGFIWIGQGAFDACSQESDSNSYDDVLLGVRSDHAHSSYIYLEAGLLYPIRVVYFNVQAHAQLQFQIVDPNGNIIDNFQNVYNFPLSAIEGCKSTTRTDDASVSTVTVIAASDTVMYSPTTDAAGSTYYVKEVVVASPTATVSQALTSSTTYTHSHAVLTGNSSATGCNFDLARVSTTPGFHATFFKYECCNVNNSKYYANSYTEQSVIGTAHGISSPNYSIHGGIHTDTIYNDVKINSGLGYVNQLTGYFYAQESGLYAFTIKNVNDGAMVWFGNSYAFSCCQPDEIPYNSDIGALIFTTGDDVTAYVHFDAGQYYPMRIVLVNILLDSQLDIMVVTPSVILHDNWSDWVYSISDVESGACPYSSSSSSSSSSATSSNTSGASSGASSGTPSNTLEHIWCFIWYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIA9</accession>
    <name>A0A1E3NIA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_59442</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016929.1">
        <property type="nucleotide sequence ID" value="XM_019163366.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NIA9"/>
    <dbReference type="GeneID" id="30180053"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="543"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="586"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="678"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="770"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1197"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1231"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1270"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1313"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1343"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1465"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="151"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="863"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1508" mass="171195" checksum="09F2611BE07E49C0" modified="2017-01-18" version="1">MSDSSNVASPAEKESNSDRSSLVYEEHHQIRDDFSDEVDYIHRALTRGQSEEHPETDVLRRLSTLSQTLSHMNAEDMKTFKIDPNDFDLQAILQFMAHRNEEQGIKVKTAEIIFKNMSVTGKNTSASIVKDVGDIFFPVYSYFRDRKEKQSSSFDFGKLPKTRKIVKDVTGFASPGTMTLVLGRPGAGCSSFLKVLCGETKTYLGYEGDVHYGGIESKDMFKEHKNQLIYNPELDVHFPYLTVEQTMQFAIGCKTPSIRINDVSRNEYINTIKDLYLTLFGLKHVEKTLVGNDFVRGVSGGQRKRVSIAEAMVTKGTVYCFDNATRGLDASTALEFTEALRTSTNITKTTSLVTIYQASENIYQLFDYVTVLYLGRQIYFGKIQNATDFFYRMGYQKPTRQTTPEFLTAITDPLARTLRPGFHKSSVPSNADEFEEYWRNSPEFAELEKIIDEKLKASNPEKTEETFKEVLNVEKQKWTSKKSPYTVNFLQQLKLCCKRRVHNLINNKSYTITLVAAAVIQSLIIGSLYYNTTRETIGSFSRGGILFFSCLYFCIMCLAETADLFMDKPILNKQYAYTMYQPSSELLAKQLVAFPVRCTAIVCFSLIIYFLSNLKREAGAFFSYFLFVNLVVQAVNSLFTLLASFMPDLSAANGINGICMMAMIVYSSYMIQRPSMYWWFKWFSYCNPVLFAFESMILMEFRGSKMECSPIQLLPRGDFYANIDPLKNQVCAFTGGALSKQEYGGQNYVDGMIYLKLAFQYTWNHMWRNFGIMFCFIVGYLVVNMILVEVYNPIVASSDKLLFIHGASIPTSLLEAIGAGEEDPEKTVGVDAKSEGKVTDDIIESQHSAATIEGQKLGSDDIFMWQNVDYVVPYDGADRKLLDNVQGYVLPGTLTALMGESGAGKTTLLNVLSRRVDVGVVSGDMLINGKGIDSSFERRTGYVQQQDLHIAELTVRESLIFSARLRRPSSVPDDEKIGYVDEVIRILNMEEYVDSLVGRTGYGLNVEQRKKLSIATELVAKPSLLLFLDEPTSGLDSQSSWAIVQVLKELAKAGQAILCTIHQPSATLFEEFDKLLLLRRGGQTVYFGDIGENSSTMLNYFQSQGGRTCSETENPAEYILEVIGAGATGDNIEDWNQKWLASKQCSELTKEVGELIERTSKLPASDNSELTARYAASTYIQFKEVYKRTFMQFYRSLDYVLAKFMLFVFGGLLAGFSFWNVKHTIVGMQNVMFACFLAVVVSSPLINQIQEKAIAARELYEVRESKSNTFHWSLLMLSQFLSEIPYGIFFSTIYFICWYFPIQLDNQPSVCGLWWFTYCVFFQLYYISLGLAIVYVSPDLPSANVLTGLALNFIISFCGVVQSPKLMPGFWKFMWRLSPFTYFMDNFLSILLHHRPVVCAEAEYNYLDPPNGSTCGEYLENYFADNSGYVRNPNDTSSCAVCQYSVGDEYLSTIGMSFHHRWRNIGFYCVYIGFNVWVMCFMYYVLRVKSWNPMSPFLSLQKRFKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIU7</accession>
    <name>A0A1E3NIU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73537</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017175.1">
        <property type="nucleotide sequence ID" value="XM_019164509.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NIU7"/>
    <dbReference type="GeneID" id="30181196"/>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="4">
        <location>
            <begin position="214"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="3">
        <location>
            <begin position="608"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="5">
        <location>
            <begin position="846"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1482" mass="169041" checksum="1F87A50BD71A145C" modified="2017-01-18" version="1">MESKDMENALLELKSDVLKSKAQDSESQLNTLLKSFDPIFTAVTAGKVPTAYRISACDTLSVWFSRSIQFFNKNKTFKKQFERLLTEERATFIFHYVIDYWNDSGAALGNALKEVFVKMLAYLTATLSPEFRNELLAKWLKTSLDLPYTMRAFYFMIEHLHKQVQPVDFILKEKPDFVESCLANIWSRALGSVVGKSIFLVLRSNYSEDTEDRWLYLWQEQVIHALADVNLRKGIESYLLPSLFLVSKSATIKFLRLVICQKNIPILLSTLKIAQDAAILIEPFLETDPDTNKPLISIDELSLLLKMKTASFRIGAFQLLVSSPKLSKIVPSVVYKKVTESLDMIFMDGDLETRNEMFSSFKKFITRIKDSTYGLHRDAHSLTKKNFSKFELEIQGKLEAVEESKHFLIDLLNYIKASLRPGSSYLKKEMSYKLLIVLIRSGLDSRIAEKYIEKTKAVSFVFSMDIYNAALMRLVIDNIMDNFEDIRSYSTEVVSMAPLSLDDYIHMTLLQSRALLMLGDIKGKEVDSGARFFKFSFSYYQSENDLNKCEGIILLLLDKIDISLLEAKRDIATACISYSIQGYFAAFKFIFEIMDYKKCSEFLVSSKVVDRLMEQSRDIWNLVKMVLQHDSPEGYLLDEFESKYTAEQEAMYGKGTQVISSYAWRSVKESTSMIDSMLRAKNSPITDHNIMQVGPLLLEQLATIRHRGAFSAVYPTFISCCTFCKSKEALKSIPEKWLFENLNLIQTRSKYITRRSAGIPYLLTAILSSDMKLVKPTFYQLMEVAQSPVDALDADMNNVNLPQVNAFNCIKKIFIDVALSEESIFYVDDAFALALNSFSSPYWAIRNCAVMLFTALQNRLFSSKKVRANYLPSYPARLFFEKFGSIRGLFYEALNEAIATGFENQAEIEKIFPILTVMSRLEPTPGYTGLEAFVPLIVHILENKNWKVREMAARSLPSIIDSSQGVSVIISKLLDNVTSSETNFNRMHGSLLAIRELILKYLTLSPEDSDKYVEEIFANNGNLKGKFESKLAVVLSDINCFPIKLTYFQILHKLKLNTTKEDSRFEKVLFAWLKTNNKLTSSLDGSKQLALKQASEILFDNMEDNKALVSECIFSPLYELQLSCIQYYDDKIKQSEFTDSITDLLVDSIWKILEAQTTWNYVKSRGLQLLKVLVVKSSKSEDAETLKLHAQKLISLIEYESNEDIKLSAMETLGSYISGLMVKDSALHVDLFNYFIGNIQGMTAENLEFVIRISALKSLVAFNEVYCQRGQDEKIKLLVAGFIFEFLTDDDDSACLLASKHLTEYVLCQKNTVLVPVEVERLMMEWFCSISDKNALKLFVNSKGFSFYDTNTLFSDIVTGDLLLFSAEKSNLERNPTDKVLELIKLIENTPLQDDVEALQNVIMPLKRNLKDITHFLANNNKTDGCFGLLSSERVFDFIFCQLLLYKSLEKLGIVSFSISNLVEILQGSKMFCHPLVLEILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ20</accession>
    <name>A0A1E3NJ20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017179.1">
        <property type="nucleotide sequence ID" value="XM_019161534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ20"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ20"/>
    <dbReference type="GeneID" id="30178221"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF15">
        <property type="entry name" value="TREHALOSE SYNTHASE COMPLEX REGULATORY SUBUNIT TPS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1171" mass="131840" checksum="FA4B20479EC06BE0" modified="2017-01-18" version="1">MTIFVASLFLPYTVHFELKPEDAADMGGNEFLGDIFEPIRKEVPKPDINPSLRRSSNLSTNDDDESNLSIIQALSHNVSSSNLSIDSNQDTYLDSSNFNGNIDDDTDSISSTSSIVVPRPKPVGALTEQVPPASPSPLLGHHLPQHPHGLHTSSNVSHLSNNSNQNVSVQDFFFNNPHKTKMTLSSTSLDDVNSLGNIHSSPYAEKLVPTSLGPTPSQPQAGRSPYGQLQGTPTLVKPKAMLRNVTPQPLVNEVLPKKPFLEPKLSSMRINRTHHSSLRNLKTASILSLDKKIGVGASSAELEGQPSNDGSDPLQQKNIMDTYNYDSVSGNYTDLLQKEEDEVMKITKDCNRIVPFGGFSRRHKFAEDMDDLFNTSPWKVVEFDKGNGSLINSIESARDSNLGDFTWIGTISIPSNIVPENIKADITDELEDNYNSNVIFLDDEVFQGHYKSFCKQILWPIFHYQIPDNPKSNAFENHSWKYYEQLNQMYANKIAEKYKKGDIIWVHDYHLMLLPLMLRKLIPDARIAFFLHISFPSSEVFRCLAQRKRILEGMLGADCITFQNEEYMAHFLQSSNRLLLADFNSTGVYYNDRLTTVSYNAIGLNFKHLDTQLKSSVVKNWKNLIEERWPNKKLIVSRDKIDQIRGLKEKLLAYERFLDDHPEYISETILILICVQSGSTDEDYKNELLAITERINSKTKNISIDQPVILLNQDIEFEQYLALLSEANVFIVSTLREGMNLTCHEFICASEKLHSPLILSEFVGSASVLTKGPLLSNPYNIRQVSGQIYQCLNMLDDEKIERWNSSFQQILNNDSETWVRKCLLDIENAYANSKSLQSYNTLEPLTKKAYESALSSISDTDGKRLYVLNLDDLTANLEIHGQTIHSYQQQLIHKTLTNLSADPNNYVYIFSIFQRSELLRLYRRVPDIGLIAENGGIIKPPKSGDWFTVVDESEKAWIPTVVDIIKAFCERLPGSYYEAEECTVMFHTETTIDIDIDYKNGLIGDLITHINELFEKDFNVHASLKSGILIVKEMNLITRALEFIMEQNKGNENLQKLPSTASAFTSPVFQARGSSIAHSPGPISPIELTKTSSSGLTSNSSSEKCYDFIFACGATTQIDEEIFSYFNSIKEYSDSVEVGEIVTVCVGQTGKSRTCAKYSLKGINNLINFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ32</accession>
    <name>A0A1E3NJ32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_142349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016701.1">
        <property type="nucleotide sequence ID" value="XM_019160284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ32"/>
    <dbReference type="GeneID" id="30176971"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="36333" checksum="D515F45C3CED9F16" modified="2017-01-18" version="1">MVGVHDYKVKDLQLILGQDLHSRENRQVALFLQELELFLAHVREHIKGKKLNHLQFSHTGNVRVWFNQIMGYVRENSGVSLGELLAQLMSSVPPEQVNAGGRTYSSLQVGKTAGMGKEALFVTLLRAREALCVLLDHCEVDKALTAVSRTEVGSLIETELKFELGVDSHAHVSLRRLPLLVDHKLYMIRRYLAHNQATVAGSGANTSKRRRTVGHDYITAALTQRVMDAMSRNRHYQGPISSFTPNRFWDGRSTSRSSANNEEDDEEVLDYQDRSSDDNDNDSANDNESDGSEEMDVESGNSSGEFDPDLFVDNPEALTSDRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ35</accession>
    <name>A0A1E3NJ35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17351</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017265.1">
        <property type="nucleotide sequence ID" value="XM_019161558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ35"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ35"/>
    <dbReference type="GeneID" id="30178245"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060917">
        <property type="term" value="P:regulation of (1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000320">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1007"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="836"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="886"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="838"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="890"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="904"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="933"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="1041"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1272" mass="140437" checksum="8592E325CA4E2CF4" modified="2017-01-18" version="1">MPYTPPSRSVSKNSSNSVLAPTKKNTADLATATATATVNNSTNDSGSASNDEAGGSSDNGAGGGSKLEASRTGTHSGVSSVSSLRQNAFHNFMNHQNDSFHNLNSNSRSKFYLLDNNSDSCDSLENTGSESNGASMSDIVPIQHSDSLPARNTIAHPALHMKILPSKAASNPSIPTKDCDVQLPTNASLEDDLLKFTTESTLSYSYTPVSINSLTMRLNVLKRSLEILLENPDWLNTSLESFPDYNIHIDNPKSVAGANLDASKFKDYLRIEKNVSSSTLQTLIQSAISSEKNNSILSNSSNSKASAASPLSTSVDSVKSNLTDSLVITPINPPFANDNTRSNSVSSITSTTSISLNSMIMKDLHEILNLLDSFEKHGLELSSDIKDKVLNLHNLSLFNSTDDSSRKNILKIKILHALATPFIEQNSTQTLAGLPIMKSSNSTLGLSGMSNGNITNLDPTKNLINNSSRQFHASSNAKTKSPKAVFTVSTESPWDLLNANDLGLLMFGFSKSSLRKMKLLDLIAPRSRDLVINRLYRNTSLVFSGEIIAVKRDNQSLAWTSVWAKRKSNLIILIFEQVVCETIDMVIEKRHSFGSDFIIGDVSKQNKSLYEKDLKAAELSSILPSLSSVLKNSSEGFFSDEDITSSHDSKTINKIRYFTLKRENGKFVPCAITSESLDIDVANEDSKDLRSLVRLNLHSLPYIAGSIVISSKNYRIESFNEAIAKNLFGTADLKNQSIDIILPDFTNLIKLALKENPNLNYQEGLVLPEHYFRKLYSWSQCSTEEEKELMFLRCAGIRGCHNDGNTITVDIQLHVANTNMYVIWITFGRSYSTKANDTTTPTATITAPLITDDHTGTAETNHSNDDSMDSNGIPSQLKLLDTHYHLDTDSQGSNSTSMSRSSSAHTINQSGSLSPSTPKANKLKSLTKLAQGSSSVSSNSGEHSPVQNSTILSPDSVKPVTFGMSSQMTCAEHLLQEEEELAFLKKNSPYFPKVIGAEKRIKKYSDFKVLKNLGQGAYGKVVFAQYKKDHLYKDCLKAIFKERILVDTWVRDRQLGTVPSEIQILYALNKHPHANIMRIIDYFEDDNCYYLETLQHGSQGSGAVDLYDIIEVKKDMTEYEAKYIYYQVVSALTHMHSRGIVHRDIKDENIIVDKDYFVKLIDFGSAAWVKDGPFGVFVGTIDYAAPEVLNGQPYEGKSQDVWAMGILLYTLIFKENPFCSVDEILDGEVKFPQYSDVSPACLELIRKILVTDITRRPTMQEIKDDPWLYGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ44</accession>
    <name>A0A1E3NJ44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase</fullName>
            <ecNumber evidence="10">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_35390</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017243.1">
        <property type="nucleotide sequence ID" value="XM_019162942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ44"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ44"/>
    <dbReference type="GeneID" id="30179629"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000003">
        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 10" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="427" mass="48438" checksum="CE2B9F2AD51276EF" modified="2017-01-18" version="1">MTTPDCGEPAGDSIVAHRPTETDMRHYYENFVPFNLLFQWLAHARVPTDDFTHREFAFEHQNGAYQRYLSFQDAAEFKNKVVRANPTRFEVGAVYSVEPKNRKTVAKSLMRPLAKELVLDIDLTDYDDVRTCCRGTAICRKCWKFVTLAVAVVDAALRHDFGVRHRVWVFSGRRGVHCWISDPRMRALRENGRRAFVEYLDVLSGGKKSRAAGPNSGVYGMKKPYHPHVERSLGLLKTHFVDVVLKEQGTWDAPDAAAALANTIPDLRLRQALLARWRAHPHTTSMDRWLDVGTLYADLAVASFDLVDWRKETILATLYPRLDVEVTRQPNHLLKAPFCVHPGTGRVCVPFDPAVRDFDPFADAPSVHGLFAQDELQWRNTGLRPGVELFRAYVSGLLKDEPRRKRARGSGDEAEAEPDGDEPSLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ69</accession>
    <name>A0A1E3NJ69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase assembly factor FMC1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_73590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017229.1">
        <property type="nucleotide sequence ID" value="XM_019164526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ69"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ69"/>
    <dbReference type="GeneID" id="30181213"/>
    <dbReference type="OrthoDB" id="15893at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039196">
        <property type="entry name" value="Fmc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015:SF1">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="16230" checksum="673160F68F776CDC" modified="2017-01-18" version="1">MSGKTLFKQMYAALEKSVASSHQTHLKEILKKQDALIHYKRMQYMNAGKKLSADEDARLADEVRERFASKLPALDVHLLAHLDQEQLHRAEAEHLQNITTFLDSQREYIELLERYNPGISMKQTDKVRKTARRVGLEVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ82</accession>
    <name>A0A1E3NJ82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="4">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_17365</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017283.1">
        <property type="nucleotide sequence ID" value="XM_019161570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ82"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ82"/>
    <dbReference type="GeneID" id="30178257"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="10">
        <location>
            <begin position="37"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="9">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="287" mass="32547" checksum="5D935AF9EA0D97E8" modified="2017-01-18" version="1">MFPKFHKLNSGHKIPSIGLGVYLTPVSIAKEISLDGLETGYRHIDSAQVYKNESEVCDAIAEWLKKDPSTNKREDIFYTTKIFDSDHGYELTKKAIEVSLERAKGIDYIDLILMHSPRSDYERRHGSWLALQEAVASGKVKNIGVSNYGIKHLKELLAYPDLKIKPAINQIEIHPWLTRTELAAFCQTEGIVVEAYSPLVRGQKFDDELLLSLAKKYSKTTAQILINWSLSKGYVTLPKTVTKSRLLPNLESDQFKLSPEDIKALDAKDEYFVTGWDPTVYPLDNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJK1</accession>
    <name>A0A1E3NJK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial zinc maintenance protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_16828</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Assembly factor required for Rieske Fe-S protein RIP1 incorporation into the cytochrome b-c1 (CIII) complex. Functions as a chaperone, binding to this subunit within the mitochondrial matrix and stabilizing it prior to its translocation and insertion into the late CIII dimeric intermediate within the mitochondrial inner membrane. Modulates the mitochondrial matrix zinc pool.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with RIP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family. MZM1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016636.1">
        <property type="nucleotide sequence ID" value="XM_019161351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJK1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJK1"/>
    <dbReference type="GeneID" id="30178038"/>
    <dbReference type="OrthoDB" id="529194at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050435">
        <property type="entry name" value="MZM1/LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749:SF1">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="132" mass="14396" checksum="D8D05B3E19DEA86C" modified="2017-01-18" version="1">MASTQRALAAYRSALRATRVAFTTDTSTLAAARTNIKQEMRAEKSSTNPDYTPAQRIELLEQVGAFLRRNIVQGVKRDDSEGRYALNIHQDTELGDNNEIKKNKSRFKASGAPPSGGGCCGGGNIELKQKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJP4</accession>
    <name>A0A1E3NJP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_128767</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017475.1">
        <property type="nucleotide sequence ID" value="XM_019159981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJP4"/>
    <dbReference type="STRING" id="763406.A0A1E3NJP4"/>
    <dbReference type="GeneID" id="30176668"/>
    <dbReference type="OrthoDB" id="17644at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="919" mass="104787" checksum="CD18F9B4D9E63C85" modified="2017-01-18" version="1">MEDQGNQKPFSFSFGKDDGKPTPSFSFNAQNQSNSNKPSLFSFGSAPEKNDSKKPNGFGFNSSNFSIPVKSMAANPPTERLNFSSETAVPNNTLPSIPPASKLRSMTSNSEKQPKSFQGFDFGAKKQEPTTQEVNPANSTEGNLISNETIKNDTTDENHKQQKEKENDFLKSYQVPSAESFIHEDDDLVDNDDDKAEEFYDMDIDEKTNLPIRRLNYEEDAKSRLSKGEHLKFAVSSKTEQGMLFVTEAEDKVAAKSSSQSNFKKEKTKLFPFELIPQLDQSSEYRTFLNNLYKEFEPLLKDRKYKHYLEPENDEYMQIGVVSTIKHNREERQIFLRKLMSFTLNYLQTLIKEKMTSDFGSFSDQWVVNYEEIINVLYLLNALHFGSEDETIVLFQQWIERIEIQPDDELLASVFQDSDKPYQNMAFWSTYVKKLLLRCSFSQLVNDLNASQYEELKDSDNELFLLVEDFISLIESYDPVLFSSDVSAFLRWKKVAVELREAANNVYAKNVMIHSELLELLSILAGSSHTIDDSSSSWYECFVGHFVYQMPSKKLIPEYIENALEIDTYEKPLGGIESWDSICVELFRGKYLTVIASLEALDKSIGTFVAVLMEASGLLERYSKDIPGDNVIDKRIASKGISSNIDRMVEDLALTYLNSQELFAIGVGILVSVGSNKSREILSEMLPTYEIKDSDDLEWVLSVCSKLKLTQTLRTIQQMQGERFYEKRLIPNALSCFAESSLPEKVVSTVWRLFEDALVNGGLDAGLASQLFDSDLSKNNTILRQSLSPLYTLYQLLRQDRPDDHGKWFRRLHALLEFAYLPGFYKCGLLLLVFDNLNKGVFDMANLVALIESINRYEMDLRRDTDLLSKSASFYSLLVHSRPAPAETAYPATLSELLFSVRRGIAMDVSFTFLDDTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJQ0</accession>
    <name>A0A1E3NJQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_72417</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017453.1">
        <property type="nucleotide sequence ID" value="XM_019164191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJQ0"/>
    <dbReference type="GeneID" id="30180878"/>
    <dbReference type="OrthoDB" id="25921at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF1">
        <property type="entry name" value="PYRIMIDINE PATHWAY REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="746" mass="85590" checksum="7CFE10A686137B23" modified="2017-01-18" version="1">MEDTTSEVEMKDDRDGEHESEQDEDRSLELGIDLDLDLDLNFNFGFDGQDRPEGVQEGSTQQINSSQQESSRRKDTFPPSLSSASSTSAKTDITQLLQMDELFTAADIEVLQKSSHRRSSTKCFRCKQKRIPCSYEFPKCTSCKRDNVECYAWVRGLRKPVPRSLPLYLEARVAELEIELKKLKQHHVIDDLEERSYSITKYAATPFLESFEIAEHDNTFASFSSFYFTSSNLPAPFDTMIDNSKRGNMKTFIEEHPPVDLSTIPRNVVEIMMSNYMDIHLPQWPVISKSELTVILNKVLDNPNVAFSFEKAVVSITMAISAALITNKSEKRALSSSSALFATTISQILETTWESKMKKLQITLLIVHYAFANPYVADIWRTLRDALRLCVDMGLHKEVESEVITVLDVDDRRRIFLVCSAMLRHLSAVLRIRFPIPQPLISVEYPTIVDDSFITEKGIDYSGPKTKAAALHFYSLRLCESEICDVLWHNKEIPGTIEEWISNMDSKIENWYLKAEEFAKVNQLRFRLITKASLQIRMRRRTPRIPEPSHLSIIKLVEALSVHVDEYLNDSQRGQVSYLLMGVYYVEEAAVNLLDVMWFQHDWIFESFSLEYLDEKLKACIQLLEKFNERWPDIGASNMTGYLDDLRRKVLAKLNKPEDVSPEDIAETSSHIEHLIFPHNGKASSNPSAFDTADLSGTILDDINYADNEFTLLNQKFSEKSNWEDHFNDNNFWNLKDLFAQMGGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJR1</accession>
    <name>A0A1E3NJR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_128514</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017464.1">
        <property type="nucleotide sequence ID" value="XM_019159975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJR1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJR1"/>
    <dbReference type="GeneID" id="30176662"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="3">
        <location>
            <begin position="262"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="546" mass="61299" checksum="E09EF13326E6C5DE" modified="2017-01-18" version="1">MAAAEANSSMENMTAISEASSHFPDNVRQLTPIEDLHSLRYHDKEYLDTVISKANRGDTFFPETVDIDRSLANSTPSPKKLERKFDYNASTPLKGGLSEAQMQPQPQLQANRPSQFSFPQKPRNIFNLSPEFVSLQPGLKGSTNIDGSINPVKGAFNLFDKSASFASGNNSSLGSRSFRRPRKRVRGANVSDISFDLTNNSSRLSFPRERRSNNESSFVSHSHSKFMQGHTLSTAMTQQRGSMSRYDYIHSLISDPQCVSNLTLLIQILLNTLMVLVFLGFSLVAFFAIKRDVDHKISSYVNDAIHKINSCKREYFRNNCAPEMRVPALEYKCNEWDTCMGKDPQSVITSMAYFEVMADCMNAFFHNISIKSLLGIGCLMLFCIIVPNILFSKFRSTTIHQNYYNGNNSHIENDTNDMLNIQTSSPMKPSGVGDQQQRVLRSAGNSTFFSPVTDKVKKPASASDHHNDNNNVSGSVRFNPNVSYSMYDYEDDSNDRGNFDVVPNQRVGDEEDNSNGDEDQDNGNNNDNEDDEDENLLANQRILLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJW8</accession>
    <name>A0A1E3NJW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycoside hydrolase family 17 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_25491</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017532.1">
        <property type="nucleotide sequence ID" value="XM_019162395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJW8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJW8"/>
    <dbReference type="GeneID" id="30179082"/>
    <dbReference type="OrthoDB" id="4082933at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF24">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="34849" checksum="0C9A49D2DD80D8B3" modified="2017-01-18" version="1" fragment="single">VPTTSSDAPATTSATSSSSEAPQQNITTSVSTSSTDAQVGAQPTAASSSSTSTSSSTSAPSPSSSGCLTKVPHAIVYSPYDNNRSCKDFSTVLSDLQLIHSKGINELRVYGNDCNYLSTILKAAKQTGFKVNQGFWISDAGVNSIDTAVDDFISYVTSGSGGFGWELFSYLTIGNEAVNSGYCSASDLISKISEVKGKLQNAGYSGKVTTSEPPVSFEKNPSLCTSSGIDFVGINPHSYFDANASADEAGSFVSGQIQIVQGACGNMDIVVTETGYPNQGDTNGRNVPSPENQLIAVQSILDNVSVDVTILTTFEDLWKLPGPYNIEQHFGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK00</accession>
    <name>A0A1E3NK00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16295</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017519.1">
        <property type="nucleotide sequence ID" value="XM_019161154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK00"/>
    <dbReference type="STRING" id="763406.A0A1E3NK00"/>
    <dbReference type="GeneID" id="30177841"/>
    <dbReference type="OrthoDB" id="999962at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005462">
        <property type="term" value="F:UDP-N-acetylglucosamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005464">
        <property type="term" value="F:UDP-xylose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF4">
        <property type="entry name" value="NUCLEOTIDE SUGAR TRANSPORTER SLC35B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40072" checksum="34BB017879F334FD" modified="2017-01-18" version="1">MGGRSSFLSRVPAFIGFTVSGCCANVYTFEKLIQQNSEITTFITFSQYLFIFICASLYLRYTSFWTKKKEAEELAARKGKSANPSSGLSYEFVVPVLSQVLTGYLGNLVFQFDLSMPSHIIFRSSGTAMTLLLGWAFWGKRYGIEKIVGSSLIAVGTIIFTLDVKSVSDGETAADAGAGGSTRVIGIVILLATTVISSLTSLFKEQWYQHGSKKLEWREVLYYNYLYGTVIYVLLIPQVVSEFHKLQLQLAAGAGAPETPGTSFTLVTFVLNWITQLCCILGVNVLIFRISALSLTIVLLVRRFLSLMLSIYVFENPVSLLGHVGVLCVFAGATIYSFGSKIRQTTRGGETRDVGEKKDKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK27</accession>
    <name>A0A1E3NK27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_72540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017584.1">
        <property type="nucleotide sequence ID" value="XM_019164229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK27"/>
    <dbReference type="STRING" id="763406.A0A1E3NK27"/>
    <dbReference type="GeneID" id="30180916"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24326" checksum="1668BFB021A96FAF" modified="2017-01-18" version="1">MTLGRNAKQVKHTVRNLVSSLIQYEHITTTPAKAKLTANYVENLIIKSKKHSTRPEALKEWKHLLYGKLYNQDITIEKMTSEFIERYANRASGFTRMLKLEPRYGDNAPQVALEMVDNTDREMMFWYMAKVVARLELQGLEADPLTKKNMEKVISSKENGEAKFRETVQICKENFFENEADLELKPRFKSHTNGFNRQTTNFEFVPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKB2</accession>
    <name>A0A1E3NKB2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_131288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017639.1">
        <property type="nucleotide sequence ID" value="XM_019160019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKB2"/>
    <dbReference type="GeneID" id="30176706"/>
    <dbReference type="OrthoDB" id="10649365at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="942" mass="108110" checksum="D02EB408E77CED6C" modified="2017-01-18" version="1">MSGQEPSVSTNELKKRIEDFLSDTKDASKTASQLYVLLASEPELSETPLPIFKGKHLILLLRMLKTADLSSAPGRNLYQVCQSSLAHLQADTPYIYRTMLHLMLGRKLNEPLLQLVLSDQTVLQQFICQAVATVDASSANHTINLLSYILNNAQQCLDTEIVDYISQSLHNCIINTECVNDKLLRTTLQLYVKLGTLAYERGDLVTSSNIDKILQFQVFLDHFSVKEYAAYFQGKDATWTENSLLSLETLAYPKLADISYTTPASNIQWKSVIDYLDLFVLVSSYSNGDNIKNTLLRPVLSFLLKLCRIVDIPIEHGHNFKICVSKILHFAYVNDLRPYENVKFFLILLSYISFSADTEVRENAFIALTALLSLEKDIVAPYIGLDDSENFNDLCLECLTEYNNEITHMYIVKLLFEKTNSRFFAFNFDELFYTIQSFANIDVIRAYNNAFTKYPEKLKKICLDQLHKSNFFSTLDTHSLNLIKLVIDLKKSIKKLDSFRLQVVPLYPGATRRETRIYLYVLLSLTAKKKLDMNDFMICDMFRYNDPDIQSCLTAIFNKFSVIDPLLNKLIDKISDQNTVLVSVVESSDTLYKDISVSDNRFRIIKACVIGNSKHFLFFLRFFANMIESTPAGFEKRPLYNLLKTVEKTYLDWEINDRILFWEELSSFSADASLSESFLKILNEFIESLKADDRHIIVNQKMNIPLVINDFENKTTEELKQIIIDKVTFHKQTTKAHLKSKKILNINKAKKSTGEEIVKILNLPLDKSDEEIKVSITVYPNGRYTGETADKSEGKKTKKLNQRDRKFLFLRNLSADKSSKIVVQGKPTVITKYSSHFIKIMTNDLFKGIFQGKSNIGKFAAHLFNSKLNFISASDEEIQQALKKFRLNCDEFTKYLFVKLVFYLDSRSLMDANLRTKLKSLNSEGGSLSEFLKEEFTYSQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKF6</accession>
    <name>A0A1E3NKF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73310</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016926.1">
        <property type="nucleotide sequence ID" value="XM_019164444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKF6"/>
    <dbReference type="STRING" id="763406.A0A1E3NKF6"/>
    <dbReference type="GeneID" id="30181131"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="33"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="36503" checksum="F5A907DCC0D73AB7" modified="2017-01-18" version="1">MNLSSDPFQVLMKGGVGFSSVAFLPGGVQIPLIGLGTAFKAGGRPEEFKRALKAALLDADYRHIDAAWYYKTESMIGEVLDDLFLKKKLDRRNLFITSKVWPCFWNDPEISLNHSLHDLRIDYLDLYIQHWPACFAKVDDEFGRVAVPTDSRKNAVIDPSGDYLVTYRKLLDIKNSTGKIKAVGVSNYSIPMLQRLMDETGTIPAVNQVELHPYLPQLELAEFCKSNGIIVEAYSPLGSLGAPILKIPLIKEMAASYNVMPVDIIINYLLKREIVCLPRSSNAERIKKGFTFIDLKDADVEKINNLGAKSPKRYIDKIWAYWND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKH8</accession>
    <name>A0A1E3NKH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-dependent bile acid permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_58905</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017760.1">
        <property type="nucleotide sequence ID" value="XM_019163327.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKH8"/>
    <dbReference type="GeneID" id="30180014"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18596">
        <property type="entry name" value="ABC_6TM_VMR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18604">
        <property type="entry name" value="ABC_6TM_VMR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000825">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF353">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN_PERMEASE VMR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="495"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="589"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="984"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1150"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1177"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1266"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1291"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="357"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="687"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="988"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1358"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1638" mass="184029" checksum="CC3B141054D9D3BF" modified="2017-01-18" version="1">MTLNHTCSHFWLVDDFTRCSRHDVLEKAVPTTLVAVSALFLLYYLVQHSVLSRSSAPLALKLDADESGRAEAGRAATGAANYDDHNGGSGDTSGDATESRSSTVANIIDRHFDIKYLTLNSDGHPKVTTKVVFRSHLERTLLVLQELILLFQTALSTAPFFVDDLAAEWDSAAVPLVRLGFWAYLLLLTTVRLVNSSTGLPLQYPNLWYHTFNLVCFYLSDSFFLFRSALLHHTLSTLAEKFYIVEFISVSLLFLIQANDHFSDRPVTVYLSEGLTPSDESQSSFFSIISFSWVGPMITSAQKVLPKLEDIWSLKLEDNAYPTILKFHRVKWHRRFSVNLFYQFRRLFFYQSVITSLEAVLMFFPSMLLKKILEYVESPETESTSLAWLFVILMVFSSPITAALSGRSLFLGRRICTRMRAIIIGEVYSKALKRRVSSFTEGKENEEDTDATTSPALTKKKETRELGSIINLIAVDAFKVSEICAYLYYFVNSFVMTIIAITILYSLLGWAALAGASTIILMMPLNYKLSLLLGDYQKEMLAVTDKRIQKLNETFQNIRIIKYFAWESKFAEQILSVRKEELSILWKRCVVWILASFVWYVTPTVVCLVAFYCYTIVEGKALTTPIAFTSLSLFNLLQTPLDQFADMLSFVVQSKVSLDRVEEFLEEPETTKYDQLVSERDANSPEVGFQNATFSWSHGGSNDFKLRDLNISFKKNKLNVVFGPTGAGKTSLLLALLGEMSLLEGKVFLPGASPREDLIVDSETGLTESVAYCSQTAWLLNGTIKENILFATPYDEKRYKAAIEACGLKRDFEILAAGDNTEIGEKGITLSGGQKQRVSLARSLYSNAAYVLLDDCLSAVDSHTAVHIYEECITGDLMKGRTCILVSHNIPLTIKEAAFAVIMDNGRVRLQGTIDELLKANAFDDETTASVMQSRTTSSADLTGLDDNTKIDSSGKLIQEETKSEGFVSWDVYSTYIHLFGNKYIWSALACFFLATQLTNIYQSYWLRNWSMAENDRFSIDAAFISNVPSRVYSTSRLAWNNLAGALKSIEWHKPILKTKYNNFDVAELTAIHSTMYYLAIYSLIGLIYSLFGALRTLVVFFGGLGVASRMFHILLDKVLGAQVRFFDSTPIGRIMNRFSKDIEGIDQELAPYAEALVISFLGCITTVVLITVITPLFLVFGVIVSLCFAYVGVLYLTLSRELKRFESITRSPINQHFTETLTGVTTIRAFGDERRFMVQNMQNIDTNNRPFFFVWVNNRWMSFRANVVGSLVVAVSSALSVIYAKHIDAGLAGISLSFAASFAMSALWLLRCYADVEININAVERVQEYINDVKEESARETENDPPPSWPEHGEIDVKGLALRYAEDLPLVIKGISFHVTPGEKVGVVGRTGAGKSTIITSFFRFIEPDKGSIIIDGVDIGKIGLKALRKGLAIIPQEPTLFTGTLRSNLDMFNESRDIDIFESMRRVGLISKDEFNRLSSLLENGESLEALHKQNKDNQENLNKFLDLEAEVTENGGNLSQGERQLICLARSLLKHPKILLLDEATASIDYETDSKIQTTIREEFSTSTILTIAHRLKTIIDYDKILVLDHGNVKEYDHPYKLITKKDSQFKSMCQDTGEFEELSRLAKEAYDAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKJ8</accession>
    <name>A0A1E3NKJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_33653</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017785.1">
        <property type="nucleotide sequence ID" value="XM_019162834.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKJ8"/>
    <dbReference type="GeneID" id="30179521"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18020">
        <property type="entry name" value="DEXHc_ASCC3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000231">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000241">
        <property type="entry name" value="Antiviral helicase SLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF13">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="274"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="503"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="1133"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1338"/>
            <end position="1549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1944" mass="221916" checksum="52D869439550FFCF" modified="2017-01-18" version="1">MATYNVQSTGSYMQAMDPMREKLSIIHLDNEEESLKLPTNFIDDDILADYDKIDESDYDESDDSGDNEAFHYEDDDLTKLFLINEIKEECNRVHLKHTEGEDVDGDTFQLFENIVDLLQKEDDDAGLQGALFDILGFEELDLITKLITSKEEILTQMNHELMTVDQIKAKVAENAKRAREQPLAKEHSVTKYPHVFMSHSAGNTISFTGKAYTLPVGTVRESFEKHEEVIIPKSETRNSLAPNIFIPIKELDFLCQGTFKGYKTLNRMQSLIYDVGYNTNENMLVCAPTGAGKTDVALLAILHTIKQFVSESETSDTDTLNVDIDYDEFKIVYVAPLKALAAEIVEKFSKKLAWLGIQVRELTGDMQLTKAEIMTTQIIVTTPEKWDVVTRKSTGDSELVQKVKLLIIDEVHLLHEDRGSVIETLVARTLRQVESTQSMIRIVGLSATLPNFVDVADFLGVNRNVGMFFFDQSFRPVPLEQQLVGVRGKAGSKQARENLEEVSFNKVMEMIKLDHQIMVFVHSRKDTTKTARNYMKMIGEQSYENYFDCRDTEKYSYFEREMGKNRNKDSRELFVGGFGIHHAGMSRSDRNLTEKMFLAGVIKVLVCTATLAWGVNLPAAAVIIKGTQIYDSKQGGFIDLGISDVIQIFGRAGRPQFEAHGIGILCTSSDRLDHYISLLLQQHPIESKLTAKLVDNLNAEISLGTVTNVEEGVQWLGYTYLYVRMKQNPFAYGIDWQELAQDPLLVQRRRNMIIDAARKLHKLQMIVFDERSFGLIPKDIGRISSDFYLLNESVEVFNEMITSQASEADVLSIISMSSEFDNVKFRDDEAKELDELRDERIVCEIGPKLESSQKKTNILLQSYISRAFVKDSALASDMSYIAQNAARICRSLFFMGLNRRWGIFTRHMLEICKAVDRQMWPFQHPMNQFQLQPPIIKLIEAKKPTIEHLRDMESGELGDLVHNKSMGPKLHKLIGRFPYLLLDVECFPITTNVMRIHVSVEADFIWNFEYHGNAQFFWILVEDSDNSNILHYEKFILNKKSFSSPHELDFMIPLSDPPPPQILVRAINDVWIGAENSVAVSFKHLIKPHNDTVKTKLLKLHPLPITALNNKNIEGIYKSKFSYFNPMQTMTFHTLYNTNSSVFVGSPTGSGKTVVAEFAIWHAFNEFPGSKVVYIAPMKALVRERVDDWRARICQNTNHKIVELTGDSLPGVQEVREADIIITTPEKFDGISRNWQSRKFVQQVSLLIMDEIHLLASDRGPILEIIVSRMNYISSFTKKPIRLLGLSTAVSNAMDMASWLGVKEGLFNFPQSVRPVPLEMYIDGFPDNLAFCPLMKTMNKPAFMAIKQHSPSKPVLIFVASRRQTRLTALDIIHLCGMEENPRRFLKINEDELAPYINKAKDDTLKLALQFGIGLHHAGLVESDRQISHKLFEQNKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYKDMDLTDILQMMGRAGRPAFDTNGIAMVFTKESKKTFYKHFLNVGFPVESSLHKVMDNHIGAEISSGTIKTRQEAMDFLTWTFLYRRAHHNPTYYGIEDISQEGISSYLSGLIDKVINNLVESKCVITTGEKLESTPFLEISSYYYLSHKTIRNLLNKVIPYASFRQCLRWLCEASEYDELPTRHGEELINMEMSQEMRYSADDLEDYEFIWDPHIKAFLLLQAYMTRIELPIADYAQDTVSVLDQSLRILQAYIDATSEMRFLSTTLNFIKVMQCIKQGCWFDDDFVYALPGLKMKKASDSEKNFDLDGSEWRTAAENSELTLKQIGSFKKKPNGMNKLADKLRVSRNKKEEFIRVASTLPTGEFKVLEQNSPEEIGVELSHENAMFRKDYRIYAPKFSKPQRETWFVILCDLAKDELYAIKRESPRRSGGGVDKVQSKLKVAEELRGREVDVLMVSDFTDLTYRGRHKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKT6</accession>
    <name>A0A1E3NKT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">separase</fullName>
            <ecNumber evidence="2">3.4.22.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16581</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.</text>
            <dbReference type="EC" id="3.4.22.49"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.22.49" evidence="2"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017867.1">
        <property type="nucleotide sequence ID" value="XM_019161266.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKT6"/>
    <dbReference type="GeneID" id="30177953"/>
    <dbReference type="OrthoDB" id="10255632at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005314">
        <property type="entry name" value="Peptidase_C50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030397">
        <property type="entry name" value="SEPARIN_core_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792">
        <property type="entry name" value="EXTRA SPINDLE POLES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792:SF0">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03568">
        <property type="entry name" value="Separin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51700">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C50" evidence="5">
        <location>
            <begin position="1434"/>
            <end position="1529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1623" mass="185426" checksum="0B5A5B32395DBE47" modified="2017-01-18" version="1">MALVENAKQKGFSTPQSVANPFLMKMIHNETLDEDIIWSYTEKMITDSKSMDSEKSWEALLTNLQILDLLISRNTNLHVLQGLICTIFQTFLYLKANSIKEDIKLLTILNYQDKDICNAAETQQRAYLQIKTSLLRIYSLDASSVKSQFKELTQNYDSNSDFYKLLVQFSFQSTEIAFALSYSFIKLSLSTKTAYKLVFLILATHYSVFTGNIKSELFDKIAKDGKQMFIKSDNLIYKLASSHLYALDRVCELKRYNVKPLDTLIEAFSKFETDKVQKKIPEKVSSFSELLQPLVNMNLSDNISTLLDSKRLDDLLSSKSFVKQFGIIIRNLDIDNSSGTQTLFKIISFLTSNPERHELVIIFLDPVLIKLKDLKCTSANTEMIHSNLEKLANFMIGINLTKKLTNLSRLFFHFGNIALMFEPEKSVIFWASFLRCDRYLCSSMSINETTLKRLYYISSDQISKANFEVAIAVQLLLLNTALSDGNVFDYESFLQHDYHTSVKLITNCLVHKSQCSKILFEHIQNESLITALVLSVVKLLESMNLGDQKEILISQLIIVQKESLNDTGLFLYFLSRISMIINFPISFKNSSLSFNLDSAVIDYENLVIPHLYILQSYSNARYSPQFLLKCCDMVKVWIKKIDCKHTEYEFEIIKCIYFALQYNNLHNICLEITRAYFNKRVGLLSKNNHTLLISFIIDSLLFLEKFMECKDFIDKNKELCHTDKIRTYEEMNLSISILQLLTKTDDLNVISKCQQIHLMLSSNEMFSISKQGDKYKAVELLIMHSKFCLVLGLYPTSGHLNSVTNINKSMSIMQSIFKNFLLQAPRTPSLNINFKSLLKIRFSLEMLYCYNSIIEQFSIVGLGKEFNHYLKELDMFIKVQPSINLQYYYNLKLLEFSTRQDAVGTASQYLQYINSIRDDICTDQNELVEIYYLMVIENYARKKGDEKTVIATSNKLDGIIFRCLKSENCNSLLIKQVATILWRRFDNFCKVGVAASQILSTKKYFELLSKLESQLTVLKSFNLSLISDSNGVSCYPLRNSGLKLTIPNNGTAELQNLNLKLINHFKDTFGFSTVEVSKSKLCMIINCFTALVSCDNLVNADNQMSRIISLSDQFWYRPFELEKELALDSQTQKNLLPTILDGPIPPFVPRDQKFSLKSVLPAEWQVLTIDYVPSTDSLVLVRHNYKSDDPLFVSISLKRKDSQHSFPNILSSLKSIIAESDKTTSPEVTKNIKSYEEKLRWWELRKALDKKLEDLLSLIDAEWFGGFNSLFQLSTASSEDVKHFKHSLVQLIAEKSRLKGIDDALIQKNLLDIHGEIYELFLNIDRINVEKVANLLHFLLGKIELEDAFRFDDDAILSVARQTKAMIVGLEKNRISNSDVPFHTVLVPGASCIQIPWESIPSLRAKSVTRMPTLFQLKDHLIKYKNLLDNGIDACKGYYVINPGGDLQRTEANLSPKFRHLDGWNGLVGEVPDDSNILKAFDEKNLYIYAGHGGGEQYIKSKNIKKRDFIPPSLLLGCSSGLLKGGGSIHPYGTAYNYINGGCPMLLVNMWDVTDKDIDLFTISALTKWGFFVDYDSFDPFDISVNNSTISLSVAEARNVCKLKYLNGAAPIIYGLPLRLETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL02</accession>
    <name>A0A1E3NL02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017930.1">
        <property type="nucleotide sequence ID" value="XM_019159841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL02"/>
    <dbReference type="GeneID" id="30176528"/>
    <dbReference type="OrthoDB" id="3998150at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56837">
        <property type="entry name" value="Colicin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="372" mass="41403" checksum="920A3053793705C7" modified="2017-01-18" version="1">MGGQMGGWTAAVGFCASFPMPLGRWRKQKVSKRIVWETTAGAQSSDCLLETTTRMKKTNGSQQYVSKGNFGHDLSNSFSNMSLERGHGKKGEKSGVEANVYVHKLSESVPQTDVLYKRAAGGGGGSVTLPAPAMQDSETYNDADDEKDTGSNTGSESVSAESELGSSDVGERSSIPIRSLPEFEKIAKSLLRQIKASRSEAKQNRDLAQQLSNDLATVTQSSAGRKASAASDSLQNFENIISFYGKKFEDMDRASKENVSRMVELEDTFNNIQRFQSSVYQNHLKELDTQISEKNRELHELRRDYQQLEQWVLALFKEVGDRAKSWSNHEFVLTTNEISKSLKPPVKKSNLEMLYRYLVQFLYLEEAEEGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL05</accession>
    <name>A0A1E3NL05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Protein URE2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_72331</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an important role in the cellular response to the nitrogen source. URE2 gene plays a major part in the repression of GLN1 and GDH2 genes by glutamine, and is required for the inactivation of glutamine synthetase. URE2 gene product may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017361.1">
        <property type="nucleotide sequence ID" value="XM_019164160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL05"/>
    <dbReference type="STRING" id="763406.A0A1E3NL05"/>
    <dbReference type="GeneID" id="30180847"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10293">
        <property type="entry name" value="GST_C_Ure2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000034">
        <property type="entry name" value="Transcriptional regulator URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017298">
        <property type="entry name" value="Ure2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037861">
        <property type="entry name" value="Prion_URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="9">
        <location>
            <begin position="69"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="10">
        <location>
            <begin position="167"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="121"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037861-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="316" mass="36215" checksum="03C62061D56FBAD8" modified="2017-01-18" version="1">MNSNNSNNISNLSAGLRNIHLSASDHHIDNEGNFSANPDINKQQQQQQQQAETETSELREFFSNPTPTDGFSLITHRSAPNGFKIAIVLSELNYNYRTIHLDFNKNDHRLPQFLQLNPNGRVPALIDHTVPNLNNVSLWESGSIIIYLVNKKCKEDNNVCPQIWSNNLIEQSQILSWLFFQTSGHAPMIGQALHFRYFHTINVPSAIDRYTDEVRRVYGVLEMALSERREALIMELDSENAESYSSGLTPLSQSRYFDSPVWLVGDRCTIADLCFVPWNYVVDRIGIDLKAEFPEVYKWTKRMMRRPAVVRALTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL56</accession>
    <name>A0A1E3NL56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72912</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017995.1">
        <property type="nucleotide sequence ID" value="XM_019164329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL56"/>
    <dbReference type="STRING" id="763406.A0A1E3NL56"/>
    <dbReference type="GeneID" id="30181016"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000048">
        <property type="entry name" value="Proteasome subunit alpha type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="250" mass="27371" checksum="F2D4BFE97D54CFDB" modified="2017-01-18" version="1">MADRYSFSLTTFSPSGKLKQIEHALAAVNQGVTSLGIKATNGIVLATEKKNPSILVNSDFINKIDKVTPDIGMTYSGMGPDFRVLVDKSRKVAHTNYKRIYNEYPPTKILVSDVAKIMQEATQNAGVRPYGVSLLVGGYDENNGFMLYQVDPSGSFFPWKAAAIGKGSNAAKTFLEKRWNDELELEDAIHIALLTLKESIEGEMNGNTVEICVIGNANDDMLGFKGTTGVSGPRFRKLTPQEIDDRLDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLB2</accession>
    <name>A0A1E3NLB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA repair and recombination protein RAD26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_34718</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017246.1">
        <property type="nucleotide sequence ID" value="XM_019162901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLB2"/>
    <dbReference type="GeneID" id="30179588"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061635">
        <property type="term" value="P:regulation of protein complex stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18000">
        <property type="entry name" value="DEXHc_ERCC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000039">
        <property type="entry name" value="DNA repair protein Rhp26/Rad26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058951">
        <property type="entry name" value="WHD_Rad26_CSB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25875">
        <property type="entry name" value="WHD_Rad26_CSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="381"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="727"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="966"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1079"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1097"/>
            <end position="1107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1193" mass="135917" checksum="9D14E9CBCFBE1C7B" modified="2017-01-18" version="1">MQEDGKLTLNENVKAESGDESYSGDKGVESAERLSDLNKADSNNQNQPGALAHLNIKILDQNDFEKQVEHDADKAIYEKELEIEEKRLAKANDKYLKFKGKLLTLQKRLHQPNLKISEKTRIGVQIEELEENEIMQSLKDLEDIKERMKKLKRNMQPVTQTQSAESNIPQQLPEETREEYLIRTGKITAFGTANAFIEENNDADRMQVPTHKNLIMPGFNNIAVVDEKDKPASIDLESIEFLADAEEEGDDGDFKIDYDEINSDDMDDDSEVIDEIYTDDDIDVTQRKKRRKKSATDELIIDNLDDGNETFYEKRLNNWVSQRSAMRLQNSPDYVDDPSVPEWYKPHPTIKDAILNDKFRLPGDIFPSLFDYQKTCVQWLSELYNQKTGGIIGDEMGLGKTIQMISFLAGLHYSQNLRGPILVVCPATVLRQWCNEFHRWWPPFRAVILHSIGEGLSKTKSNKRSAEDDEDDEDLYDLENEDYGSVPTLGRTKDNKVVRELVEKVVKNGHVLITTYAGVRMYAKYLIPVKWGYVVLDEGHKIRNPDSFITITCKQLRTPNRIILSGTPIQNNLIELWSLFDFVFPGRLGTLPVFQRQFCVPINLGGYANATNVQVQAGYKCAVVLKDLVSPYLLRRVKTDVAKDLPKKTEMVLFCKLTPEQRRLYKKFIDSADLKRILEGKRNALFGIDMLRKICNHPNLVDLNLKDKKITKLPSIEELSSKSGKIQVVMALLELWKREDRKTLIFTQTKQMLNILQQLLDLLNESSEGGYKYMRMDGSTPIIQRQSLVDQFNLNPQYKVFLLTTRVGGLGVNLTGASRVIIYDPDWNPSTDMQARERAWRLGQKQDVAIYRLIMASSIEEKIYHRQIFKQFLTNKILKDPKQKRFFKMTDMYDLFTLGDDDVKGTETADLFGADEQTFDGIKERKTKFKSRLTNNKSNTHASPEVKDEGDDDFLKATRLAGVSGLEEYNDEQMREKSMFDDDDSRTQDSSASGGNEKNIMSEIFKKTGIHSAVEHDSILDKGNYNSGLASSMALIDNEATRIANDAVAALKESRSLTKKSNFAVPTWTGKFGAAGKFNSVNNSKRRKLPQSQTPFSERSSSPASSNSILANLRAKKEATNKRPASQSQTLITQLSSYMAGVDGNFSKSSQILEHLDVDLSDEKTVKVVRNMLKSICSWDKEKKGWVLNPEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLF7</accession>
    <name>A0A1E3NLF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16767</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018077.1">
        <property type="nucleotide sequence ID" value="XM_019161333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLF7"/>
    <dbReference type="GeneID" id="30178020"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160189">
        <property type="term" value="C:peroxisomal-mitochondrial contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160190">
        <property type="term" value="F:peroxisome-mitochondrion membrane tether activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990627">
        <property type="term" value="P:mitochondrial inner membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990626">
        <property type="term" value="P:mitochondrial outer membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000638">
        <property type="entry name" value="Transmembrane GTPase Fzo1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF0">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="13">
        <location>
            <begin position="229"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="452"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="875" mass="99155" checksum="30BAA0CF53A21694" modified="2017-01-18" version="1">MTITDKNFDKDSESYQNMGGTDMDKSYSENYAFDDDSLDDKTLLNGSNNENGNHGGKDLHLPAIANYLVQNGTLEGTTLNDGEAGEQGPILPSHSNYEDPSVAHVKQLKYNQNKILLDRAINSTVSMFTEFKENNINSPIHYPIDQMISNGYDSKLNSISSKRALMLQEEDDNTTKNVKFDILKLELKVGMKSSENLLTNLDDNTISMLLDEKFMQIIHHLKNLKARIDDITSKVLITGDLNSGKSSFCNALLRRNVMPVDQQPCTNVFCEIINYQENNSIEEVHAVPIGSSYNIKDENTYNKFSLKMLDDLVYENDKYSILKVYINDNRSVDESLLKNGVVDIRLIDAPGLNLDSYQTMQLFSRQEEIDLVIFVVNAENHFTLSGREFISNVNNDKKFTFIVVNKFDSIKRKDKCVEKIMQQVGDLSPETHKDSDDFVHFVSSSEVLNNKSLLRGGDDGPGGDPNDPNGPGGNRPSDRNFDRLEASLRNFVLDKRSASKLLPAKNYLTKIFKETLLLVNMNIDNYKKSKEKLSTELNEIKPKYDEIVKISVKVNEKVMTIIDECSDDVYSTTRENINKTIQSFAEVKEVDKSVTLFNIDQFIQATKLKVVHDILSCIEDSEDYARLQTLHSIQDIRSLGKKVLGDEKLPEHKFKPESMYSHKRDHQLKNQINISFELLDLIDFDSILGFFKSIQSLFDFTNISKFSKISSEDFTLSSILSSNVLKTGVSSILILYGPKLVSVFTGVSSIIKLPKVITFTSVGLLIGYPIYYIVKESPKTVHKNIIKKIKSNLVKDDYIHLNSLRISKEVRKVLNYPAKDVSVGINGLIERESNLKLKLVNKVNLLKGNLKFLQSLKERVEVQKQIVDGFELEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLF9</accession>
    <name>A0A1E3NLF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_22783</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017521.1">
        <property type="nucleotide sequence ID" value="XM_019162277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLF9"/>
    <dbReference type="GeneID" id="30178964"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000140">
        <property type="entry name" value="Beta-glucan synthesis-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="177"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="582" mass="65830" checksum="46892DB2AF4148FA" modified="2017-01-18" version="1" fragment="single">NDNSKTTEYDRYPKRLSGSRVSSIHSSNSKTPSYVNKSNMDTDSFSNSSDRSSNPFLVDTDFSPFGGYPASSFPLMIDEKEDDDYLHNPDPIQDAEYEKHRIWYDFKSMDKRYSLALLGLLFFLFGGAALFVVLPAVTYTNVAGHHDKLSANRNSTVGSGAVILSDYVYPQLSAIRTDMVDPDTPTQFHTRKAKDGSLWKLAFSDEFAAEGRTFYDGDDQFWYAPDFHYDATKDLEWYDPDAVTTKNGTAHLRMDVFKNHNLYYRSGMLHTWNRMCFTQGIMEISAKMPNYGNVTGLWPGMWSMGNLGRPGYMATTDGVWPYSYDSCDAGITPNQSSPDGINFLPGQRLSSCTCEGDEHPNRGTGRGAPEIDIIEGEVDTNIRVGVASQSMQIAPMDIWYMPDYNWIAIHNNSITTMNTYAGGPFQQAVSATTTMNTTWYELGNSTSYFQKYAFEYLNDNDDGYLRWFIGDDPTFTVYANALAPNGNIGWRRISKEPMSVVVNLGISQNWAYIDFPSIKFPATFEIDYVRIYQPNDSISITCDPEDYPTVDYIDAHPKAYNIWNYTLWNQTGYSTPRNNLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLG4</accession>
    <name>A0A1E3NLG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_19114</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018092.1">
        <property type="nucleotide sequence ID" value="XM_019162106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLG4"/>
    <dbReference type="GeneID" id="30178793"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071920">
        <property type="term" value="C:cleavage body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033620">
        <property type="term" value="C:Mei2 nuclear dot complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990251">
        <property type="term" value="C:nuclear exosome focus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106222">
        <property type="term" value="F:lncRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140517">
        <property type="term" value="F:protein-RNA adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033621">
        <property type="term" value="P:nuclear mRNA surveillance of meiosis-specific transcripts"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12306">
        <property type="entry name" value="RRM_II_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="10"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="131" mass="14440" checksum="42332DDB42F5F532" modified="2017-01-18" version="1" fragment="single">IQERQEIDNRSIYVGNIDYAATPEEVQELFKECGTVNRITILTDRVTGTPMGYGFVEFESQESIPKALEHNQALFKGRPLKVIQKRTNVPGFGRGRGRGRGRGRGGFRGGFRGRGGRGAFRGGRGGFPPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLH7</accession>
    <name>A0A1E3NLH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">COQ4</name>
        <name evidence="8" type="ORF">PICMEDRAFT_33828</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the COQ4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018100.1">
        <property type="nucleotide sequence ID" value="XM_019162847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLH7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLH7"/>
    <dbReference type="GeneID" id="30179534"/>
    <dbReference type="OrthoDB" id="4249at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="280" mass="33104" checksum="02E5AA696C5E9685" modified="2017-01-18" version="1">MERNEVHVKKTHNEIELEKHDQLKKSGVPKSFKRPPKLYPNHVPLFTYEKLLLFLGSSIGAFLHPERNEFIVALGESTATKGFLLKLRSDMLHDPVGREILKERPYITSEHLHLEKLKSYPENSFGKCYHDWLIREHCSPDTRVDVKFIDDEELAYIFQRYRQCHDFYHALIGLPVFREGEIALKFFEYLNIGIPFGGLGALFAPWNVKKSSERERLFKIYYPWALKNAYHCKKNLINIYWEKILDKDVDELRSELNIELPPDMRALRKKQRQATKPHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLQ7</accession>
    <name>A0A1E3NLQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Maintenance of mitochondrial morphology protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MMM1</name>
        <name evidence="12" type="ORF">PICMEDRAFT_15061</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all outer membrane beta-barrel proteins, and acts in a late step after the SAM complex. The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex. Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. A MMM1 homodimer associates with one molecule of MDM12 on each side in a pairwise head-to-tail manner, and the SMP-LTD domains of MMM1 and MDM12 generate a continuous hydrophobic tunnel for phospholipid trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MMM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018174.1">
        <property type="nucleotide sequence ID" value="XM_019160646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLQ7"/>
    <dbReference type="GeneID" id="30177333"/>
    <dbReference type="OrthoDB" id="5599157at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21671">
        <property type="entry name" value="SMP_Mmm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03103">
        <property type="entry name" value="Mmm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027537">
        <property type="entry name" value="Mmm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019411">
        <property type="entry name" value="MMM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF0">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10296">
        <property type="entry name" value="MMM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="144"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="240"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="396"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="550" mass="61013" checksum="E9ADE28EE53DEDEF" modified="2017-01-18" version="1">MSIEIIPKDSSRKSEVGILDQETVFSTSAVNNANPDKTTAGTDVIVNSSSDITELERYLSGILNGLQKQQETQKNEVLQLILQQHQELQGEQNQQPQLQSHSMSLNSMASLPHSTAWTFTQGLILGQLSVIILFIAFIRFFVFTEAAPTQASSIPSKSNVSIIKAKKGDDMDLSRLQGGFSKDHEKEVASSIPKVNGLTDDLSHQKEINQALGSGHIKLDSNSTLISILEKTYYDVKTHKPESLDWFNVLVAQIISQARLEALNDGNIYESLNKALNSPQVLQYFDRIKISEINIGDDYPIFSNCRVKNNEGRLEAKIDVDVSDTITLGVETNLLINQPKFMSASLPIKLSISVVRFSACLTVSLITVDDDYANPSNDKNHMTSSTDDIRASFTEFKSNDTPDISGAHQDTNSGSESTPAFVDNHENREAGSFFNSPETNTNPDNRATNNEGSSIPNGDEASSESSHYSEGGHSIALMFSFSPDFRLEFETKSLIGSRAKLENIPRVSSILENVIRKWFIERCIEPRFQLIKLPSMWPRKKNTRESVPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLS5</accession>
    <name>A0A1E3NLS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_10117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018194.1">
        <property type="nucleotide sequence ID" value="XM_019159631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLS5"/>
    <dbReference type="GeneID" id="30176318"/>
    <dbReference type="OrthoDB" id="10419429at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="519" mass="60950" checksum="253BD481B77C6A80" modified="2017-01-18" version="1">MEAVCTVVGSLPPELYAYVLNKTIQTLFTKTEIIRFLMLNEEFCKDIWIHDLTVTITDRGYLKFDEDPLSNDLINLRDFIFGNVKIFAKLFETHTISIQNLILHANVHQLAFLANYAVYIQCESFNALCMSMNLEIDMNKVRMITLSFYKNHFAQFYTMAKNISILPCSAHYVFNFAISDHHDFKEVINILHYCSTKLHLRYTIRSKVLFFLNMTISNDSKLPTNKLIKYFSYCLFLLEEYKVSTTDIKVVLKISLADPSLRKSDEFLKLCKYIGFQFIHKLIIESDDDGYYQFDFRDIRQLTSLRLLEVRCDQQCGFENFGDLSNLKYLRKLVFQCNNVELGWLSHNLPHSVETVQLFRTLKNNEEKDKSIFEIPDFLQNLIIETDLPEVVIDFERFKFNPTIRLQRIIILDHFVKNPAVKILNLQQIPRCLKEFRFHDSNNFTSSLHENYFKFPGGFDSSQLEGVYSNIDVEFTSLQSNKIYMCLCSYSLRILNLKSKRIRNFRGCTKKSIQPSPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLT7</accession>
    <name>A0A1E3NLT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15091</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018213.1">
        <property type="nucleotide sequence ID" value="XM_019160660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLT7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLT7"/>
    <dbReference type="GeneID" id="30177347"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000421">
        <property type="entry name" value="Inorganic phosphate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004738">
        <property type="entry name" value="Phos_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00887">
        <property type="entry name" value="2A0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="49"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="564" mass="61566" checksum="9EFCA0549E6223E2" modified="2017-01-18" version="1">MSGKLTKGGNAAFHNFINDFADVEDPLERKRLALEKIDNADFSWYHVRAIMVAGVGFMTDSYDIFAINMSVPMMAYVFWKGSIPSSTQTLIKVSTSVGTVIGQVGFGWLADVLGRKAIYGIELIIMIAATILQCTAGHSSAVNFAAILTFYRIIMGIGIGGDYPLSSIITSEFATTKWRGAIMGAVFANQGWGQLMAGIVAIICVAGYKNELDVVGAKNCGPDCVKACDQMWRIVIGFGAVPGLLALYFRLTIPESPRYTFDVSNEIEKATADTAKFTSGKLGNAETGDIDYLAQTETRDQALNYQPPKASWNDFWAHFLQWRYGKILLGTAGSWFMLDVAYYGLGLNTATILKTIGYASSTNVYKSLYNQAAGNLILICAGSIPGYWLSVATVDVIGRKFVQFWGFTILTVLFCIIGFAYHKLSEGGLFALYILCQLFQNFGPNTTTFIVPGECYPTRYRSTAHGLSAAAGKVGAIIAQTCIGTLQDRGCVAKGLKKGCWLNHVMEIFALFMLLGMLTTLCIPETKRKTLEELAEELHGEVDITKQRVHSIPSDDEDKIADSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLW8</accession>
    <name>A0A1E3NLW8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15128</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018245.1">
        <property type="nucleotide sequence ID" value="XM_019160679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLW8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLW8"/>
    <dbReference type="GeneID" id="30177366"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="323"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="324"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="388" mass="43176" checksum="DE44EA9ACB374730" modified="2017-01-18" version="1">MPPMSPYPYQMYPHPYPEHQFHHQFQPQLPHQLPHQLQLQFPPHHPYHMASSSSPTSSTSSPLSTKEHDNRGPYESHNLSQGLKIPPPLAVQSLPPDTLQVPLSTPTKQMFFHPQFSAHYNSKANDSVSAVSSTTASACSASDSASIDSSNTTINSETSTPGIYGPSSLSPLHYATKKQVAPPLPPLPQMPHFPQLQLQQQQQQQHVQYCAPSPAPVASNKEKSNIDGTAFPQTFDLSVLTPIPGCMAYRHPLIPVIPSASGNTRKQTRRLFDAKTNSITEGPIPDSSVSTTASAGHDNDSDSSSIDSSAATQARRMAFGSPTWTQKDDELLRHLKEVEKIGWRDISMYFPTRTINACQFRWRRLIMKEENRRKRELHKSALRARLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM66</accession>
    <name>A0A1E3NM66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enhancer of polycomb-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_25813</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in gene silencing by neighboring heterochromatin, blockage of the silencing spreading along the chromosome, and required for cell cycle progression through G2/M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017556.1">
        <property type="nucleotide sequence ID" value="XM_019162415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM66"/>
    <dbReference type="STRING" id="763406.A0A1E3NM66"/>
    <dbReference type="GeneID" id="30179102"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032777">
        <property type="term" value="C:piccolo histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="359"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="784" mass="91415" checksum="DE4EF5B347B27AA6" modified="2017-01-18" version="1" fragment="single">MGSTSTPGARFRQRKISVKQTLLVLKQKDIPDFDIEDQQRELQHFETGVEKGEEEEHHLQQVINASAAKFKGANVEQVYIPTPDASKVWKDASHYYHQSFNEPDSYIKFSATVEDTTGCPYNMDEVDDEFLQELNEKFPKEEPCTEDEFELIANKLDMTVDERQPFLTMDPQQILSFKEIKEYAFDPKSLSSDLHQTLANKLHVKTFKTLYDAEPSSGKEPRPLPVLFDLFGEQIYDHWKNRRIERGGKPVFPTLKFEDPSQKDDNNPYTCFRRREFRQARKTRRTDVQSIERLKLLYKDLRRAKSLFFSVAEKEVKKSELLQVESETFSLRNELKKIKRDENIKLDDDLLIDKPKKKRIPLGYVPPPPTPVKSETDNIKDKIKNKDGSSGRGSLKKQLKADGATNKRNSTKNLNGVNGNNFSNNSNSNGHGHPLNQSTKTPQVVIQPYVKLPPSKIPDIELTTVNTVLKDKLEGIKKAVSDKLIKRRLQDEGWINFTDDPYNPYFDMSNSGEDEIEQRSHLPYSSIISSMFEVENSREINFSHIFNNNRHYSNNDSEIVKVNLFTGQVVKNDRHSYLPEFYDLVGENPTSNEFGETREEIEKQFNDKNRFNVSEVLFKIRKRPGRGGRIWMDRKRITDDDLFEEYLNMPEDEDEENEELEEKLFDDNEKMDVDKEDDAAEEKLESIKEPPRGTKRISAYDCGADSRKRLKSRFMFDADLPKMNPLDPSKLNQIGKQTQAIRFGCMLLSKAYDNMHQIRQKQIIHHQQKLQQQQQQREQFQREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM93</accession>
    <name>A0A1E3NM93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Bile pigment transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_10269</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018370.1">
        <property type="nucleotide sequence ID" value="XM_019159654.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NM93"/>
    <dbReference type="GeneID" id="30176341"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="542"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="977"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1020"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1099"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1123"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1212"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="292"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="624"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="984"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1308"/>
            <end position="1559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1580" mass="176660" checksum="AE4A971310B9515B" modified="2017-01-18" version="1">MDFVANSLGCNPKAPLISPDSNAISPCFIALLFALLAGVTGITGSYQLVKYTKFTPTFGTLISETSHQALKFFLILLQAVTIGILFIGEAINSGISFQLYAYSFAVLELLFVIFPLHLIETSKSNIPTATVLFYWPLQFCLLLAVYVQQLFASDEKKIFPNGSELEPFLLFFSLLMNSIVFIMEYCYYRPNRELSEHYKDKRSVLSEPDVFSKLTFSWMNRLLQLASDNGGLEENDLPPPPEIVSTFYSAPRLEKNWTFELNNASKSQKPSLIVALIKTFGMGVLGSLVFDMAESFVSFIEPQLLRKLIQFFNVKDSNPLLIKGLTIAILMFLTNLLSTAFYNQYMIITVEVSQGVKSALMYLVYNKAMKLSPTERNNRSTGDIINHMSVDITRIESLASYVQTMVSAPTRLVLCLWSLYWLLGKSTFVGIITMAIMIPLNTILVKSLRKFHKQQMELKDKRTSFVSELLQNIKSIKLYAWEGPMLSKLRYLRDEKELKNLNKIGILSAGINFAWTCVPFFVSCSTFAVFAIVSDIPLSPEIVFPALSLFDLLSDPIFAIPALITALIECHVSLTRIVSFLTADEIDLDIVKRLPAVKKLGKKAVEVSNATFIWNKQIDNSVETGNHDIEAEITGTKAPALKDINFSASKGSLTCILGKVGSGKSTLLKCLLGELPVVSANSADPYNLSISGSVAYCAQVPWIINATFKQNILFGHKFDETFYNKTIEACQLLTDLEILPDGHETLVGEKGISLSGGQKARLSLARAVYARADVYFLDDILSAVDAHVGQKIIDNVLGKNGVLKSKTKILSTNSIKVLSEASTVYLLENNTISETGTMKEIMTNKSSELYRLIKEYGSKNSNYESYLDKPLNEPKSNFSSSMSASSSSIDTKLLVEGELEGVEPLGINVDENTGEELSRFDRKLLNMNSSSTLKAASIASFKQLTSFDDVDRRMTQTEEKKEKGKVKWSTFSRYAKACSYSGIFITLSLVIVTVGMSIGSNYWLMYWSESNIKKGSNKDIFFYVGIYALLGIGSGFFTLIRAMCMWTLCSIRASKKLHSDMAYAVLNSPLQFFETTPLGRIINRFSQDMSKVDSALPRVFSSLFSSIVKTLLTMIIIGYNMPFFILVILGLSFVYNYYQNFYVITIRDLKRIVSITKSPIFALIQESLNGCETIRAYGQRKRFSFLNSKNIDNNQVSSYCMKSTNRWLSTRLQFIGSIITLSTSLMALGSLYSKHRMSAGLIGLIMSYAMRVTSSLSFIVRRSVEIESDVICCERIFEYCKLAPEKDEEAIKDQLVTPPKDWPSEGEIEFDHYSAKYRENLDPVLKDISFSIQSKEKIGVVGRTGAGKSSLVLSIFRLIEATSGHVEIDGINTSDLRLFDLRKNLSIIPQDAQCIEGSVRYNLDPLDQYDDEHIWDALTMSRLDNHVRSMAVEQDMPDRPLECQISESGGNLSVGQRQLLCLARVLLKTGKAENGTSEPSKILILDEATSSIDTETDNVIQQTIRTRCNHLTILTIAHRLNTIMDSDRILVLDKGRIAEFDTPERLLQSTDSLFYKLCVNGGYIPKPDDWAESEDNSAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMJ0</accession>
    <name>A0A1E3NMJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PhoD-like phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018462.1">
        <property type="nucleotide sequence ID" value="XM_019163921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMJ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NMJ0"/>
    <dbReference type="GeneID" id="30180608"/>
    <dbReference type="OrthoDB" id="2419400at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF1">
        <property type="entry name" value="PHOD-LIKE PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="183"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="468"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="711" mass="79334" checksum="277B3F505B67D35C" modified="2017-01-18" version="1">MTTAEPAVPAKETATGPGAAAGVETDAQGSWFAPLPVHEYLQDVRNAVPDKIPEENPCPPDYALGELSVACGPAVRFLGTHENNSDSYRGSVLLVVRDYAGAEGTAPGIEFRLGPAHESDGPVGGGEFLKVAATATELIHQEQQFSFFRYSFTFELTQYEQRVRYSFDGLSRPHFQFFLPTAAQSMNVMSFSCNGFSLGTDTATFKGSLWLDVLRKHNRPNFHYHVMIGGGDQIYADGIKNISHEFNAWLKHKHIHSADKLTPKLEASFDDYYLNRYIEWFGKGYWAGTAGRTIQTALPIALAAIPQVNIYDDHDIIDGFGSYTDVTMRQAIFQGVGQHAFRYYMLFQHHTPYRESPKNEPSWIMGKAPGPYITEPSRSVYTRLGASIGFLGLDCRTERTKHQIVTQSTYDLAFKRVEQEILDSKHAGKPIKHLYVLLGVPICYPRMVFLERLIDSPLFYPIILLARKGIIAHSLVNEFDGQVELLDDMNDHWCSHTHKKERNKLMKKMIDFGKAYDVRITILSGDVHLCCASRFRGTSEISQANPRNDPSFITNLISSAIVNAPPPDGMAKFLSMRARKHRFQKGVVEDMIPLFSVEPGSGKHRSHELFMNKRNYSDLIPVANIPTDLLLQRYGDTATDKFYVPGVVGDKITFSNKTDNIAAASKTNGPIGYPLDPDGVVATLRVEADMTDVNSETGAYELLVPSLEVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMK5</accession>
    <name>A0A1E3NMK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017961.1">
        <property type="nucleotide sequence ID" value="XM_019159848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMK5"/>
    <dbReference type="STRING" id="763406.A0A1E3NMK5"/>
    <dbReference type="GeneID" id="30176535"/>
    <dbReference type="OrthoDB" id="4068815at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014839">
        <property type="entry name" value="Crt10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08728">
        <property type="entry name" value="CRT10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="893" mass="101357" checksum="A034E4ECAEBDC712" modified="2017-01-18" version="1">MHHGSVMSEDEMSDLDRNHDLTFEEYLNSRGWQSIRKSSLNDREADTSADTSGSFHGDNLRYNLFPDSTDQLDSFDGEVDRLSDLSFRTSNTRGEDRNDLLYEQHQHEYQQAREQQQRPVFRQERNGGGSDQYYSSSDEDDDVVRLTDSITNEFEVSAAYYNDSLHLDLTGDRPIRESQFIYSKTDGNNKGFYRKVTECTKVSHLDHTNGIPLNPKDSLENYNRAEHLGLNNTTVDRVLQSSDPMFAMSNQPKAKFHPSTVLDVQGLGQTKLELQPQFRSPSVYFKSNLSATYCKNGYQVLVVAIGCNLHFYNMSGISTHDSETSSSSSFISKTDFPFIGNLNLEFRNETNTQVENSVNPLERYSINYLTIKTFRDPANNTKYDVLAVCNDFSQVIVLDMKEIMNHFFSSDYKAATETETFNWLFFNDDSMVFECVCANKKNSFFRSTLKAFDKPLYVNASAWCVDICDAMVAVSDNLRRITVFQQSSIREKRMKYQSSVLSHNIPYLNIVRNGVENYLICCGTYGSHQCILSLTSNKVGEYSLHVLDVVEADGPVWTCNFVKESDFMDVSSLKELTGDLVTDLDGQKLDKIITQSSILDTGRDFAGLYSSHIGLNAYLTHLNIPSITSRHSQKKSKSKTMAVNEINQLERVRKLYSEWYKARRMPTDSGLRREKSKLAAAKLHETNHLFNYFIVTTTTQSVGLFLMRELINLGTCKQVFPIIESPYIVERPNTNEDTDNDINASGRTDVAAILSLNEYVSFSSGANRRNPILPPRYQFLNRIYLSFPVLSLNAIVIATQAGQVAVFRLTKFNGLHSMRLESVLIDAEMYAMAQDGRLRSIIGLTGSVCGYDNTGESEWMIVVVYSDMLTLSYKLHKNKRAGDEGRLSVLSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMQ4</accession>
    <name>A0A1E3NMQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018527.1">
        <property type="nucleotide sequence ID" value="XM_019160793.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NMQ4"/>
    <dbReference type="GeneID" id="30177480"/>
    <dbReference type="OrthoDB" id="498590at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="3">
        <location>
            <begin position="393"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1195"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="325"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1203"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1274"/>
            <end position="1292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1292" mass="148072" checksum="78C57338971857B2" modified="2017-01-18" version="1">MLSGSSRLRRPRNSSTRAKAKSTDALTILDKNVNYSKRSNKGDKANSGGTGKRRPATYDSDIDEESENNESNDDDADEDEMLDDDNDDNDNSGDDINDDEEDEDSYVPNRKQNKRRKGSAKTSNRSGRSNRYASAKSKSLVSNIDDVENYTENPLFESLSNGDYSPADLAQSWIDDFVALDMNTKFDALKDFLNFILRCSGCIVQFNRHDVTNTESAKDTVNELQTLFARQKYHEFPMMYAPAGGNKDWKEFPDNAVAFISQIIVIAGETGSLYEEEDQFVELLLEWISAMSTSNIRALRYVSTLFGLTIQSVLCKLSVNISKFIDKFLRQLKKENDSLKSLVENPKSSNRQLKRQVDSANERIKVIEDNLEMYKKQKKSIDGYITDFFNTLFVHRYRDTAPEIRSKCVNYLGEWMEDYPELFFESTYLRYLGWLLTDQDSTIRSEVFKVLIKLYKKRSTVTALRQFTSYFKNKLIEIVIYETDFSARLNCLQLLNEIIEKGYLNDDDIICLTSLIFVDDEDLIYPFQGSKLNPGKFMKELAIFVSKVEHLNNTEILERDEKELETINESLPFDCKRILKIKTLLTILSDSYEYYLTRYSTSGIKKKIENSKIEKFSNIFQYLYHLRTYNENNEIFELLLTYTNFDFSAYAISEEIKENFELDSKLQTLLLSLINGATMIYSQGAENRFHKALFPNFTRKKNSLANSDKDSNYYVLKLLSKLPDICYYFHDDIEKISILVHLTSSLLKYKPVEVTNKGLIETISQFFRFFLVINFPLATKDDQDSKYYDSITHQYISFFKLVKTEELDLISQLDSILTEVNMTLQSKVIMKSSTIIENLNKLYILCYCPYITNRVIKEFNSVLPLFSNSLYFSVEHDERYIRQKDAILLSLTKSEISAFLRISSFYTNISLLQIYEGKLHHHDDILTLNSDTIGTINCIQRNLSLLIEPDSSTLSMDLVHDIAASYLDNVLCISAFKAEFGSLINQASSSTDVEVSEIQLLRSHIGKSIQQQLMKVFCVREYQLAELLGTEAQLERDADEDVNFSNYSVMKLDSEDLDADVEEGEDEEETGEESEYSQRSNKKLEKKNEKYQLLLCEIASKIILCCNLELVDDTNDRTIIIERVKKNSEFLGELFKKVLAGVGLLVTYNSDVTLTGIKSKTGKGSAKAVQHQLVGETGKLAKIIKRGKKRRLSDGSMLNFSGVDDEQEQEGEDEDDPIENSDVDDDNDQEVEIGAEKEEQDETEVTPQADEDIEFSGIDDDDSVLNNSRSGRRAITTSSEISSFASSFPFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMS9</accession>
    <name>A0A1E3NMS9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72739</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017806.1">
        <property type="nucleotide sequence ID" value="XM_019164282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMS9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMS9"/>
    <dbReference type="GeneID" id="30180969"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="503" mass="56191" checksum="A7E0A174D3671A47" modified="2017-01-18" version="1">MEEKTYIQSLIAQYLKDAGLNKTLTALEQEVNKKFTTPVHDESLESIVRDRINFQALSQFSDPDQNVNTLDVFTQSDTIKAKNIVLPNWSIKYPDSCEFLNLGTLNSLIIYSSFFQAMVREKVYNLGLFVTNTRCIFVIDMDSSQILLSSLDPLHGQTIKIIWGVTMTDHIIVCDMNGSVTSMKFTVDGEDNWDLVASTTTEGSSSLKLHRRLITDFKYIRFTSDENPSLLGYFASIGWDGRVTVGRAQRVGEEIKFSIIGDYKLFTNPTSLLLTEDRNTHLPVVLVGRLESSLLTMFTITSHNPSASKLVEVAKLSLNDSEFSSHSFQPMAIAEISSIDDDIIITIATDHVPYMRLITVLVPSLKEIFVTELQNNGEQPFSSPDILSLLKEKASKSEENIESFHSKTLILRSYIISNFNSLSPQDKYSNAIILCRPHCSGLWIPGDDGMLRGFDLRTGTVVETLKSNDGRAKSAFIGKLGNDSEVIVVCGAVDKKISIWKCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN51</accession>
    <name>A0A1E3NN51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit B</fullName>
            <shortName evidence="6">eIF3b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 90 kDa subunit homolog</fullName>
            <shortName evidence="6">eIF3 p90</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor eIF3, p90 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PRT1</name>
        <name evidence="9" type="ORF">PICMEDRAFT_31362</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="6">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the eIF-3 subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018645.1">
        <property type="nucleotide sequence ID" value="XM_019162697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN51"/>
    <dbReference type="STRING" id="763406.A0A1E3NN51"/>
    <dbReference type="GeneID" id="30179384"/>
    <dbReference type="OrthoDB" id="10250414at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12278">
        <property type="entry name" value="RRM_eIF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03001">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034363">
        <property type="entry name" value="eIF3B_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036424">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="37"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="729" mass="83454" checksum="2272383474EEE8CD" modified="2017-01-18" version="1">MSVAIPEFDEKSVDISGLDFSDLEEKYDVKAPKMIDNCVICDGAPIAPEAKAPVLKKVLTKLFSPCGKIEDLYLPLENGKTKGYLIIRFSNAMEAAKAVAQLNGKKLDVKHRLLVNKLTDIDEYVLSGKIQDDFEAPEFPPFTEIGHLNSWLLDPKGRDQFLMNFDQSVGVYWYRKNLENEPVIEPRMQWTSTYMKWSPKGKYLFSMFPQGVQSWGGPEFQRIHRHLHPGVRLIDFSPNEKYLVTLSPEPIMDPMELREELRKDYPFKAEDIGKKLVIWDISIGVPVKTFALPPDLENATRMPWPLIKWSYDDKYCARQGPDAVAIYETEKDFQLLDKKLLKIEGVQDFEFAPAGVRLVNSKKTDPLDVIISYWTPETENQSAKVSVMQIPNKAVLRTIPLVQVQDIKLNWHDSGKYLCCNVTRHTKSKKTFFSNLHIFQLFEKDIPVDKVELQELVLNFKWENRSNKFVTISKSDKGELGASNLGAESNTTTFFDVEEVRVGTGKNLTTLKKWVPLTSISNSFEDISWAPQGRFVILTSFGLASTQLKFFDVEFDGDKPKDQNKKVQSNLKLLTTHNFAGLCELQWESSGRFVTAVSSTWKTKVNNGYTIYDCAGHVLKEEQIDAFKEFQWRPRPESLLTSSDKKRVRKNLKEYAAQFEESDTMDTNAALRDLILKRKQLLAEWTEYRSKTVANLASLNLPDHKGTVAEETIETIKEVVLEEHEEVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN73</accession>
    <name>A0A1E3NN73_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_10540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018670.1">
        <property type="nucleotide sequence ID" value="XM_019159686.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NN73"/>
    <dbReference type="GeneID" id="30176373"/>
    <dbReference type="OrthoDB" id="3998282at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="964"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1465"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1631"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1755"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="785"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="964"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="979"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1336"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1349"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1360"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1500"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1514"/>
            <end position="1524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1529"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1554"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1577"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1644"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1672"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1755"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1812"/>
            <end position="1841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1851"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1868"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1904"/>
            <end position="1935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1935" mass="216951" checksum="9F8DAB4AC0479100" modified="2017-01-18" version="1">MYENSANKFVNSNSKQENGSNQSKKLIIRLSRKNKLMDQATRRHYANNPNVEFYYEEDRIMDDPEYWITIAERANIPIRFGNPQSDNNLQISGTESTHFSNDKNSLLKSLQERFKAYKQMSKDDEDAYYANIEREYKLQKERVKQAEFNEKTRQQRISKLFRQLGYSVSIDKEAGNLSSSSSCVKSLPASEGEESNLAISQVETRISDNEPADDTDDSIIILSDPEYNEHAGEVTHNGILNDFIGEADVGNDTSEDEGEDEGEDEGEDEDEDNSDIYSFEPMSEYDTKLVNENDNTFRYAANGTELKSDYRTAYNFPSNRGFGIQDIRHQDQEVSELSGEDEEEENIKVREESAELDELEEMEEYIEEEEEEEEEEEEAENAVDVKGDFDSKVAKQSSSVFNLPEEEIPNYSYDRKLEKVAVNDSRGTLFNREFSNPRDNATESIYHEFYMQEKNTAPSNDNYVTLDSDSENFSSDKSDNNEKADIEADTEDHLEIYKDDEKSTGGEIKDGGSNDVSLEEQHQIDMSDAAYAYELARLSQQYQNDIDMIVNETLEGAQKTDEKTQSNQNSNSHESDTRLLDIPQDTNFFTVHSDSEETRKDLLMDKSKAENLVTDDSVIDQNLFFSAIENSELHESTPVVADEEMKSVSSPVKYKPDNILVTPSTPPTRVIDFKPITPPLPPTPDPPQLYMGLAELLNASLDIHSKQSAGEIIEERLVDTAQKLVEEVSTAFGKIESSELDHFLSAPINKPTFIEESGSNDRKHPFSDIEELSNDACHNGSSGIFRERQQEDISQKEEKDEKRNKINSGESRAERVRQNSKEVDGSESDEISKEDNTTEHMVVKDEPTVLRNLKEASPFHDLLQNNESFNALSESSAFGILSTKNEEERENSTKTFREEDYINSLLNETLADSRDLSSAPLSGGVYGETIMESTDELSDADQSFYLTDGIKDEDKKSVYIPDKTKYRAKDESGNERYPATNTEQSPKSLPQSIDTSCDTGTFSLKRHYEEMVKEEFPSPNSFVKRVKGVISKISSFSWYDKGPDPVIESLNMKDNESLLTNIGVQEQNIEAEVDLNDSAVSQSTKDNSIVNVPKDPADSHHESSLEEIEDSDARVDTIIDAAEGLSEKTKASDNEVEMEINDFPKEVDEIEVSINHLENEEYNIFHTDDASIETPEAAVSDLKSSNENISPLEIDLRKRDETENIRIDMSANNEKACEPVDEELQLIAEPMTENNEHSKDEPDIMVSKQMENNENHNETIVNENKAYSNKIERQEDKKSWPFNLANALVEFSNSALSEACFEESDDVGIDVEATDLEEEKEENKGEEGVNGTLPIAETSSAQAESSQPSDDELSDDNTVNEEHEVSGLKKDVSFERTAPSIAGSETEGDAGVKDSSVSQHAFDEESVVKSSIKGKDDFFSEGETTDVIVVNESPANVPFKNEVEAKTAFPSKVVKLFTGLFSESKLSKKQTPTAEVDPTSAGISHSESEPETGVLPNDESSASTSDADTSTIHIEVKGEKDASESRSGSACESESEANTEATSDAEVSVMLVHENTNTESSSTDSESSTEIITVREGLSDEIHVGEETGDEGSTDMNVKPEATEIMRVIELPSKESEPILKVEDTTNITVRGKNGEDDLLSTQKDFDNEPQKRGRSEIVKMGNEDIRDDDEQPSKRLHVENSRLFVPPVAVRTRSRSPSRKSSESSQRTLSIEEGTKTSINVIANRVLSYGSDDDPVELKEDQLPRMYPNLIEEADHFIEHDAEKKPAGVGAFETNSDDEENESNAQNEREKTLKSPQIVVTKRKSSSQDASSERRSDRILRNDDVKIIDEKPSEETEARLTRRTRSSKKPSTDKDETKPTDEGEKDEKEDDDLDATFEERLKTSLNKRPNSKSEVTDEAPSRGRGRGRGRGRGRGRGRGRGRARGRGKGRTKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN74</accession>
    <name>A0A1E3NN74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018672.1">
        <property type="nucleotide sequence ID" value="XM_019160846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN74"/>
    <dbReference type="STRING" id="763406.A0A1E3NN74"/>
    <dbReference type="GeneID" id="30177533"/>
    <dbReference type="OrthoDB" id="5595751at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390">
        <property type="entry name" value="UROPORPHYRINOGEN III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390:SF0">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="259" mass="28892" checksum="4B4DDE9D5A800800" modified="2017-01-18" version="1">MSKKVVLLKNETIPFDPYKTDFKNRSVDALFIPLLIHQPINHGEIVKFLTSSEYLDNFQAFIITSQRCIESLDIVLSSIEDTNILQKILNKPAYTVGPATSEVLAKLGFEDIRGGENAGNGSILSELIVADTFFQNGNKKILFLTGEIRKDIIPVKLTSAGFDLKELVAYRTEVMKDVVERYSSVYNNLSSSDCEKWLVFFSPQGTEYITEHLLSRNSDFKIASIGPSTEAYLLEKGIKPDFVAKKPNSVSLVQGILDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN81</accession>
    <name>A0A1E3NN81_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_33706</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018016.1">
        <property type="nucleotide sequence ID" value="XM_019162837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN81"/>
    <dbReference type="STRING" id="763406.A0A1E3NN81"/>
    <dbReference type="GeneID" id="30179524"/>
    <dbReference type="OrthoDB" id="10255148at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="204" mass="24282" checksum="32D67D5E7FBD3F03" modified="2017-01-18" version="1">MGAYKYLEELQRKKQSDVMRFLLRVRCWEYRQYKVIHRASKPSRPEKARKLGYKAKQGFVIYRVRVRRGNRKRPVPKGATYGKPTNQGVNHLKYQKSLQVTAEERVGRRASNLRVLNSYWVNEDSTYKYFEVILVDPSHNAIRNDPTYNWIANPVHKHREARGLTSAGKKSRGINKGHLYNKTSNGRRHNWKKHNTLSLWRYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNH4</accession>
    <name>A0A1E3NNH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15580</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018768.1">
        <property type="nucleotide sequence ID" value="XM_019160879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNH4"/>
    <dbReference type="GeneID" id="30177566"/>
    <dbReference type="OrthoDB" id="1746530at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="203" mass="23906" checksum="6E7C1BDF84B48678" modified="2017-01-18" version="1">MKIIQQRDKLLSNFEVYQHIVDVQKENSWGFTIPKVEKKRKQKFDPRLLDLEIITRDLSQYLVKMDQGNEVKTENFVNLMLVLNTFELEVIEKLMIMNSLPRSLVNLYAIIEECEGRFSQDQCEDMLQNVERYFPAPVNEEEEEADEGEEIGEDFEMQDAGVHVAMDEEENDDEAAFVQADEFEHEGFKKKQNEEKEIDEVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNI9</accession>
    <name>A0A1E3NNI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018773.1">
        <property type="nucleotide sequence ID" value="XM_019160883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNI9"/>
    <dbReference type="GeneID" id="30177570"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="56" mass="6576" checksum="E725578E0A7C6152" modified="2017-01-18" version="1">MHCVYLVGCLANTHYTQFVLSTPRSPKPSPHENEKGIHRVRYPSQRQLHFDRVTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNK0</accession>
    <name>A0A1E3NNK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15229</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018361.1">
        <property type="nucleotide sequence ID" value="XM_019160727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNK0"/>
    <dbReference type="STRING" id="763406.A0A1E3NNK0"/>
    <dbReference type="GeneID" id="30177414"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002058">
        <property type="entry name" value="YML081W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF13">
        <property type="entry name" value="RESPIRATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="468"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="480"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="495"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1278" mass="143422" checksum="00417A88D0FA1F2C" modified="2017-01-18" version="1">MDSPRSASNKRNLESAILTDGIEQQQLQQQQLQQQQGIHDSFLEDDEDEYEQDFGAPLKKVASSKSSSGSKMIGKPRPHVCSICTRAFARLEHLKRHERSHTNEKPFQCAACGRCFARRDLVLRHQQKLHASLPTNNRTNIQRKGRAQKLLSKDGEIVRDYLNDNINIVRNNTSAKLPIASSAAPAANYTSTGTNDVSPAISNSTASNNVSSISPLSDSSNSNNSVKPGHLLNTTTMNKNNNMYSLSQGHSLLSSTNPLMNQMDFFNSNILGSSITNSAAYNSPTSLNGLMFSPNIYTNNKNGSNAINKVDFNKPLGHENITPESQQTKRVDSKTDDKFDDLNEDDLDSDLDSEEHEYDNPQAMYAHDLANLNDISAEDEASSNYRNQRHASFSAAASSSYTGLPDSKIPHDKQMEELEKEAPQQVNFSSPQLLFKPNSELGYLDLDILENIDLNDLGLDFSTFNFSNDMNVNKHSTAHNSDENEKRRDKIKDPSGNGSSSISATSGNKVGSNGSEDMSPHEISLQKVFSNPQNGVALQKVLSHPRSGQALQRVLSHPNSDLALQKVLSHPEPNLVLEKVLSHPSDTDWFNEFINAPIETNFPMVSDHIGFTDTPDAADSPLSGTNLTPGNSQNILTGSNILPSNANDLKNLFRSRQIDLSKHIPHTYQNPTIPGRCSESIRRHIMAKYNLKQQQFPQLDDLNHYLALYELEFSVYFPLIHIPSFRIDDNLDQIPLMLSMAAIGALYSFHARNSSTLFNFSRFLIHNFMEVQMKLNRFNDIPLHITQALVLHMFLGMFHNDSEITKLISRQLNSLVSLVKITKLDMPLESLLLPPSITFDIVQSNNLDLIEACYKYFILAQSRIRTIHVLHHITVLYGCLTDGKVEMSADNIESGSPCANEDLWNCKNFNEWVAILKEKNVNINSKFSLIRLSNGHAYKELWRGMQNLSLDQDIGLRGMLSLLFTINEYVHTESRKLEHEDKTDGAKLAEWRMEQRPYIESLIKIWESCYIRNGGVLVPRGQNIHTINSSCVLKLTLPLLSFAKINKCIYISSVLSNVWKRNWDAMNLEIKSLTRDVEALRDSVNYSLDIINLWIEIISINNDAEKTSIRTPIFFLTCLFTSTLLISEYLYVTEVWANNYLSNKDKDANLTTADRVLWLRAEKIFKKIEKNLLPLGSNNHSYSEFLRIQANGALDVDILDDEIARLALEPGDLRPIAEIIISGRLSTRCLSLGVRILADAPVWPIALLFAEALKARAIHIHDCTSSWSPGPASASLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNL3</accession>
    <name>A0A1E3NNL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial</fullName>
            <ecNumber evidence="4">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ubiquinone biosynthesis methyltransferase COQ5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">COQ5</name>
        <name evidence="5" type="ORF">PICMEDRAFT_58089</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-methionine = a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.201" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018792.1">
        <property type="nucleotide sequence ID" value="XM_019163279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNL3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNL3"/>
    <dbReference type="GeneID" id="30179966"/>
    <dbReference type="OrthoDB" id="6329284at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000133">
        <property type="entry name" value="2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="159"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="290" mass="32407" checksum="2DA71B182241BA42" modified="2017-01-18" version="1">MSVKLSTLHRLGAIGSKTAATPRRLFSQIDFTHFGYKTVPKGEKERLVHDVFSSVASKYDVMNDVMSMGVHRLWKKHLIEKLDCGMRPNSGELLAFLDVAGGTGDIAFGLLDHARLKYGDNKSTMTVADINPDMLREGEKRCLSTPYANSARIQFLEQNGETMDAIPDNSKDVYTIAFGIRNFTNVQAGLNTAYRVLKPGGIFACLEFSQVEEPALDYLYQIYSFSLLPMMGQLIANDRDSYQYLVESIRKFPNQETFANMIREAGFYVPGKGYENLTFGVAAIHIGVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNU9</accession>
    <name>A0A1E3NNU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_111676</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018471.1">
        <property type="nucleotide sequence ID" value="XM_019159766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNU9"/>
    <dbReference type="GeneID" id="30176453"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="73" mass="8457" checksum="3061A4C3C4273B3A" modified="2017-01-18" version="1">MSNPEELIAEVCILFSESRPTPISRCDLKLISSRYTNKHTHRSTNFIASGQQEAEEELWAVFLLSRRLQCFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNZ4</accession>
    <name>A0A1E3NNZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_21435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018915.1">
        <property type="nucleotide sequence ID" value="XM_019162202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNZ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNZ4"/>
    <dbReference type="GeneID" id="30178889"/>
    <dbReference type="OrthoDB" id="1914839at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="664" mass="74398" checksum="EB928A992898E79F" modified="2017-01-18" version="1" fragment="single">VYYFLEFISFLIFAVSTLSLMLRLLIDSEQNYEYSKSFTASESNNSPHKYRDSFSSSLQCFQSKDLIQGHVLDIVKLCTSSCPFIVSVGIDHKVLVWSPLNHPIPIPTQLPISAKFLPITHVEMSDSGSAITVFSRSGEVKCWSRSSMSWVWTILFEELANDTPLVSFFRKRTQISNGRRKLISRVSRQTKEHTPTHSTLSSRHPPPTSSAETNTDLSSGRRLSLDSNFDQNITTKDASNMEFIIVLKNGAIISIECSTGKYDRAVLSDSPLLCAKKLISPRVNDRIVGVKENGELVVSTVINNKWRSRPVKIDTSNYNKGISLITPAVLLKYPEFNYSTSPSVLPAAKSFAATSNTDKRVGFVDESLNDLQGIVMETVPFVGMIVRAFGSKCQLIDVQSGTVLKEWQIFQFKASTFRVFHPEPSHCRFCGCASVASFSVAYTGLEGSSLIMHTFSIDNRAKNNICLRVERDSRETRCLGFASVTEHQHVLGNVEGWCPTDMNILMGVRRKENSEKIDETEGDNIETEEKVSPIIENSSIFNRVTNTAQTTHTHRLSDKWEGWTMSADGQVRFYEIPDGSDSGLLIKKLGPVSKFGHKSLVVSFGNIMKVLYLGNDSLVEEDSPGDEDLSSARYQSSSSLGFINRRRRMRMKKYDLTHSTCFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPB9</accession>
    <name>A0A1E3NPB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_10390</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the NDC80 complex, which consists of NDC80, NUF2, SPC24 and SPC25.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
            <location evidence="11">Centromere</location>
            <location evidence="11">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018506.1">
        <property type="nucleotide sequence ID" value="XM_019159663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPB9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPB9"/>
    <dbReference type="GeneID" id="30176350"/>
    <dbReference type="OrthoDB" id="7459479at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.30:FF:000001">
        <property type="entry name" value="Probable kinetochore protein ndc80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 11" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="14">
        <location>
            <begin position="181"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="147"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="451"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="617"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="753" mass="86743" checksum="AB65606C8DF4AF80" modified="2017-01-18" version="1">MSQTGQPKQRTVQRQNSFLSPITSTTFTNRRLSSSRSSLLRSPDGTESSRDRKRKRLSLLPANAHLGAPRLINPPNLRRQSIMNNNNNNNNSHNHNHNGNISSIPSLNSALQAQLASNSTSSTPFTIRNAATQNTVQQLVQNSITRSGIPLNTQRRSTIGSRRESVLPPSSASGNRYETPAPAHRPSAYSKPQDPRPLRSHRFQSEVQDELYNYLSTKKFDIEMKQPLTPKTLKTPTQKEFVLIFQWLYKKLDPGFKFNKSIEQDVYTLLKFLDYPYLDTINKSQISAVGGSNWPVFLGMLHWLLKLVKETLKFDDLDIYSFQEEQSNKTDTNLTDDPVISNEISLMNKLFLTYVLDSYRAFLTTGEDDYSSYFAEMENEYLVYIEEVQSKMNIDEELHETLQQKLDSSKETYNLFFDEMERANALQTDVTKFQNYIDIQKQRQLKWPSVIEKANSDIRNIMESIQNINKEKQDIISDLEKKNLTLKDIEDLHKDRARLTTSLNLIDSKQRQTKQLIESKSEALKLQFNDLQAKINFYNNTVYQILNDLSLETPPDTSSLVINSLDEEYQSTKIGTSPHEMVPILPKLRSSLGDLKNKVQSHITKLQDDILQSQETVDDLKLSIVSCTDKLEELEDSLSKSRKEYSELNDKYTTDSSNKQFDLEEKAKEIRLFKLQNTENRKSIESKWKDAQRDYKKTISMISENRTQLVCDIAQCLDYVVSFKSDVMTDLENTAVEVSQELKQQLDAESQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ1</accession>
    <name>A0A1E3NPQ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018801.1">
        <property type="nucleotide sequence ID" value="XM_019160890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ1"/>
    <dbReference type="GeneID" id="30177577"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="79" mass="9584" checksum="577278F36F5D75CD" modified="2017-01-18" version="1">MNRHERSLHLKTTFKCFGKCNRQKWGCGHLYSRKDGLRKHWKSAQGQECLREFMVLNNLTEHYHEVKDLEKIIELTHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX1</accession>
    <name>A0A1E3NPX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019253.1">
        <property type="nucleotide sequence ID" value="XM_019159745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPX1"/>
    <dbReference type="GeneID" id="30176432"/>
    <dbReference type="OrthoDB" id="5431245at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037738">
        <property type="entry name" value="Ecm13-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36826">
        <property type="entry name" value="PROTEIN ECM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36826:SF1">
        <property type="entry name" value="PROTEIN ECM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="348" mass="38459" checksum="1C43157BFCBB53A6" modified="2017-01-18" version="1">MMSSYFQSTYNSGRIAQTYLLASKVKAKLTQEAVRNDVDLHRLVCQANLLDNLIENLNHHESSYNSNTSSNPGKTNVCYDSINSNSYPLLNNVTVQVNDVDYVVDGDADSGEEDDEVDENEIKIERAPCSDVYYTSDDSDFDSDDDSDSSFNSDTFGGKSSDPNELCCIALQRLNLHGGVEDGLGEDDDDLENQATCSSVTATITLSGSDSDSESNSETESDTEYENENENDSRDHGSFSNNYCALMRMHSQHTSLNLNDYVASLEADTSVEETDACTNIPHNSSLDEMTREEDLPSLSNCSSFSSMEEIGQPNQNIVRLDCLDSIKHQHDTDSPSLYLTQSQDVFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ85</accession>
    <name>A0A1E3NQ85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15621</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018816.1">
        <property type="nucleotide sequence ID" value="XM_019160894.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NQ85"/>
    <dbReference type="GeneID" id="30177581"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="462"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="63"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="183"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="260"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="427"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="503"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="988"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1141"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1248"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1309"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="748"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="274"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1156"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1248"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1265"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1309"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1335"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1346"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1379"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1398"/>
            <end position="1407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1502" mass="164534" checksum="819ED7E3B93E5DBA" modified="2017-01-18" version="1">MPAGNVSVSKQGKGGAEVGHQDDKGLYMDSEGGQAPKHGAGVFAKPEALGNAETNNSPYMMNAPSKSPTHLHTQPQPQSMNNHNHNHNLNHNSDGHGHGRGNSVITPSALPFDLSNWEDAPDLNFLLDSQNASVESPNLSSALSPMNNINTPHNAALGSFNGLSQLNNLNFRGLASKQANETMANLPSNPNSGSSQNAATGRNPSTNQTSSTPVDYAMQLQHYQNLLLETQSLLNMKQLQQKSPASMDQSSTFSKAHNTLQQTLQQHQKHQDQHSSQASTQAQGQSKGQGQNQSQSQKHNAAALLSANQLDPNNFQNFVPNFNANSQALLQQQILQLAQMSNNKLFSAPFNPSLAAAAAAAGLPTGMQNVPVSSLPQESMALPSYLTNSLIALSTPAIPVPSPLSQNATGLPSNEINNNNNISNLSINPATGKPTSSTISSSPTEFLKRKADMPQPRTKVRPCDHCRRRKTKCVMFAELHSCKACQQKGIKCTFNEASTIKRSMSSSDQDNKRMKFEDPTIEPPPNIPVRDTHPIKDYSTMQGHSLLKKTLSLQFPRSSFYVGPTSIYDPLFLDEISLDKIDQFQINKSNSIRKVANSIQFLLRDDFSELLYEQSERESDTVEKYVAPNGHILIDLYFRTVHPSFPVLHKKIFLEKYARTHREFGASILAAVYILAIQWWDYEPRLANLPKPDVTSLLRFAMRTFIDVTRRPKLSGVQAGLLLLQCQSLNVSPANNTANELGSEKVSVKSEDKEKIKAKGKNKALEKEIENQIQMSQQNNWLLCTQVVGLAEELGLGLDCSTWRLPKWERGLRRRLAWAVYIQDKWSSLVESRPSHIQENTNWIVKPLTDEDFPERNSSFNESVEKGNDGEQATEGYTYVDVELGKESFKQTVSLSLILSEIQFTLYTPRAMEDIKQIDELLKLAKPLQLKLRQWYHSLPKTLQMGSTSPKQFNANGNLQLSYFATEITLHRRIISILYNQMLMSQNSQGNSDSQSPNSPVLSSSAAESNSSTTNGGSTNIPAPPAQLVFVCRNAAKTRLTASIEFVRELRTEHLHSFWHSSAPRNFALIGIFAALLYVTSSDADEASAYREYIMDYRWILKLNSPDFVIAKNALELIDGVVRNIPGLWNEYDGVSIDHMSNIGGPGGDSGGLDPNMSSTLNNSEDPNNDHTRTNSSRPKNTDSKGNKSNSNGNGVSDAEINTSNSAFKASQASLVAVSNNTSINNATKGPASYNYDLSTAISPRTIISPTRSNSDVSKVRGYKNKTSNSNTPVSASFPPSPLQNGNTENKNAKLISRSKSKSPILLMSQQRVSNSSGTPVLSNTEISSSNVLQTPTATDTSTPTLMSQTRISTPTRSTASFSQQTRNSGNTDSASIDGRSDINEDKAMSKDDEESTSADGNVEENNQAVKKKVEKLFKYDGQQSILNMTTALGITKDNNGGMGPIGKLKPVSYNGSMNFEGTNKKASPEAVKRGEQQTGMSLDMLNHVQKLLGLGNENGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQE1</accession>
    <name>A0A1E3NQE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_13924</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019440.1">
        <property type="nucleotide sequence ID" value="XM_019160135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQE1"/>
    <dbReference type="STRING" id="763406.A0A1E3NQE1"/>
    <dbReference type="GeneID" id="30176822"/>
    <dbReference type="OrthoDB" id="5380163at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000067">
        <property type="entry name" value="BZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013910">
        <property type="entry name" value="TF_PAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023167">
        <property type="entry name" value="Yap1_redox_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08601">
        <property type="entry name" value="PAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111430">
        <property type="entry name" value="YAP1 redox domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="87"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="229"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="248"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="533" mass="59116" checksum="D9D9A7F5C0665A5F" modified="2017-01-18" version="1">MANTKTQDHAAVEKPHTKPGRKPLTSEPKNKRTAQNRAAQRAFRERKEKRMLELEDKVRALEQEKMQIANESELLRIQVKQLMGQLGRNGTTGHIQGTGMRSETYPEELEREDSSSVRRDSSSDEKSTNNTSSNSSVSSATPVSFAEEGYNFTKPLPDTASRNPTTDYNLNLSNKLNNKSFKDHYDEQIFCKELGEVCGSKSCPFPKDKKAYSSSTSPGTIVGNNQKYGSTMSMTSNGNNTSGSSSNIAYQSASTMTTPSVDSSSRSNNTKSPFTSSLFDGSVNGDQDILAEIGKDEINANGNEDIKGNNNSIWNIGKGLDSYAFLDSNKNNEMDFLFDDGKKVSHDTNTLIQSHPEAFQLDANSSLFNDNLTPDFDADVDFDNDDVFDDLLKLPYDDLNFNNQLSELDHKQGDNKSGNASGVNNREAVTDLVTDENEEVPDTNQNLMQCSQIWERITTHPRFTDLDIDNLCDELKSKAKCSESGVVVDGGDVGKLLHQATCEKQKQKERERELAIKRAAINSEANTFLNGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQG3</accession>
    <name>A0A1E3NQG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock protein 60</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_69993</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019460.1">
        <property type="nucleotide sequence ID" value="XM_019163526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQG3"/>
    <dbReference type="GeneID" id="30180213"/>
    <dbReference type="OrthoDB" id="1733909at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="60580" checksum="072127595C4E82E3" modified="2017-01-18" version="1">MLRFASPAVKQSIKAQALKQQVRSVSYKELKFGVEGRAALLKGVETLADAVSVTLGPKGRNVLIEQPFGAPKITKDGVTVARSIVLQDKFENLGAKLLQDVASKTNESAGDGTTSATVLGRSIFTESVKNVAAGCNPMDLRRGSQAAVEAVIKFLSEHKKEVTTSAEIAQVATISANGDAHIGGMLANAMAKVGKEGVITIKEGKTLEDELQVTEGMRFDRGYISPYFITDAKSGKVEFENPLLLLSEKKISSIQDILPSLEISNKLRRPLLVVAEDIDGEALAACILNKLRGQVQVCCVKAPGFGDNRKNILGDVAILTGGTVFTEELDIKPENAAPELLGTAGSITITKEDTVVLNGAGQKDTILQRCEQIRGAIEDAATSDYEKEKLHERLAKLSGGVAVIRVGGASEVEVGEKKDRYDDALCATRAAVQEGILPGGGTALIKATQVLDSVKTENFDQKLGVDIIKAAILKPAKKIVENAGGEGSVVVGKLLDQYSGDFNVGYDSAKGEYVDMIQTGIIDPFKVVKNGLVDASGVASLLATTECAIVDVPEPKGAPAAAPGGMGGMGGMPGMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQI2</accession>
    <name>A0A1E3NQI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DHHA2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_8915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019480.1">
        <property type="nucleotide sequence ID" value="XM_019165038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQI2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQI2"/>
    <dbReference type="GeneID" id="30181725"/>
    <dbReference type="OrthoDB" id="374045at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF39">
        <property type="entry name" value="EG:152A3.5 PROTEIN (FBGN0003116_PN PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="5">
        <location>
            <begin position="251"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="406" mass="42835" checksum="05CED7661F746698" modified="2017-01-18" version="1">MGAERKSLVEFVRYARSCYDTCRQARPRVLDVVIGNTGGDMDSVVSALGYSYLSYLAGGDGAAAVVLPVLSFARADLALRRDVERSLHAVGLRPADLIFADELAADDDAGAAGDQYRVRLVDHNAADNALVQAALAQQRAAVVAVVDHHADAGLYAAAAPRVVRACGSCSSLVLQHFAAAFASRYHARTGLAGHDGGDPQARLRRRELQFLVSALAVDTDCLQHRVEAADRDVYTTLVAPVLDAAAFAAVGTAATAAKLDIAGLSVYDLLRKDYKEYETASGARVGISSVACSLARLQQQQQQGADAVAGAVADWSHRRNLQLCVVMTSFVDKTPAGDVFRREIALAPPGFLPARALSCLKRELGLEAGPTVDADFAKATGLAVFEQHAVGKSRKAVAPLVVGLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQP8</accession>
    <name>A0A1E3NQP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 15 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_8914</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019479.1">
        <property type="nucleotide sequence ID" value="XM_019165037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQP8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQP8"/>
    <dbReference type="GeneID" id="30181724"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF7">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="54182" checksum="CFF1945B7DB64631" modified="2017-01-18" version="1">MFVFRRITRYAIFALLALGCLYVFSRSGGSGSITVIDPAAIVAEAPVSVPAAVAELDPQPHAEAAPDDNLNGKDTPVDTTPVHANALESLHFKHHKDTPREKVVEDNYNQLVSILQQVISEPKVDNLVRPINPSTYKLANATLMIMARNQDLRGVLYTMRQIEEKFNSKFHYPYVILNDGDFKDNFIDRVTKMTTSKVYFEKIEPEEWNQPDWIDSSKQKSQMKVLKQQNIAYANKVSYHNMCRYYSINFFNHPRMREFKYYWRFEPNTDYFCDIDYDVFKFMEMNNKVYGFVISLYDAQETMPSLWPETLKFLNKNKNYLNKNAAIDFLMEDMQNPVKTEFTGGYSTCHFWSNFEIADMDFYRGEAYTEWTKHLDQTGGFYYERWGDAPVHTLGVGLFADRNDVHWFRDIGYKHHPYTNCPHSDKCHGCEPGLFTYEHLKDQNCLTNWWNYEMDDTSRQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQW6</accession>
    <name>A0A1E3NQW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14027</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019568.1">
        <property type="nucleotide sequence ID" value="XM_019160192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQW6"/>
    <dbReference type="STRING" id="763406.A0A1E3NQW6"/>
    <dbReference type="GeneID" id="30176879"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF6">
        <property type="entry name" value="U11_U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 35 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21971" checksum="CA35623A3C3A15EE" modified="2017-01-18" version="1">MSTEKIFEPTVPIQRFKNTVPPQVRLGTFEIRHSGLADFTGALKEYHREQQTHAGAAGPNQPSGRGARSTQSREQVEQRRQEAVISYEERKGSAAQQFGDPYCTVFVAHLAPDTTQDDVAAAVRAVLPDLPATLPHVALVRRRARRAYAFVTLGSRADARQLLSLGRLDVLGRRVVLDVERGRIVKNWLPKRLRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR14</accession>
    <name>A0A1E3NR14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MMS19 nucleotide excision repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70154</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MET18/MMS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019639.1">
        <property type="nucleotide sequence ID" value="XM_019163573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR14"/>
    <dbReference type="STRING" id="763406.A0A1E3NR14"/>
    <dbReference type="GeneID" id="30180260"/>
    <dbReference type="OrthoDB" id="342900at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039920">
        <property type="entry name" value="MMS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024687">
        <property type="entry name" value="MMS19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029240">
        <property type="entry name" value="MMS19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891">
        <property type="entry name" value="DNA REPAIR/TRANSCRIPTION PROTEIN MET18/MMS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891:SF0">
        <property type="entry name" value="MMS19 NUCLEOTIDE EXCISION REPAIR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12460">
        <property type="entry name" value="MMS19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14500">
        <property type="entry name" value="MMS19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MMS19 N-terminal" evidence="7">
        <location>
            <begin position="42"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="MMS19 C-terminal" evidence="6">
        <location>
            <begin position="567"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1067" mass="120165" checksum="C5D789491E8758A6" modified="2017-01-18" version="1">MSSHKEYINEYIASSDPTVRQNVVDELVKLMQNNKLTLLNFIHALQIYITSTNDTTRVSTFSLLSQLLGKLPSTKLFPKDIEVLMAFLYSKLLDRPVVKYVLSSMYSLLTMKYFNYENTEELMQKLIENYNPKEHPQSIRLLALKIVNYQIASIPAQVYPNDLAIDCFLHVSQNEKDPNNLFLIFQVLQKISKTLDITNHIQPLFDTMFRYYPISFKSSTEAQESQVNSLKDSLNSSLASSDLYAVELFPNLISKYNSATSSQVKLDILTTIGVISSLYSSRVIQENFLTLWNTLKYTIINQELAQLISIPAVMSYYEESSNESDQIFHSSLIAIRSLSTNVSNDQKLLVYDDLSKNLILSERNRRFLQSYLTLAIISLPSDKKKSDDDDDDDDDVLRKTLSALFSSDQPLDQVRNKRMILVALSYFSSDPTFISQLVPFRDEILNILQSSLSTSALETTLRTLAVQLTAHLILSPSIISPSTGIEFGLLDEERSVLIGKLGELLIENGLLPSRDFNVVIEKALLIALSKLAKSSRCENDILNEVANIILLQFNNSESSLHEKCVLLNYLIKLAQTPSLVQIISIRLVNLLPHDGHDLNEFDIPVELILQALLSLFTSLPLTHDMNTITKKFLPLLSAYIFKPDSNVNEVQLTYVCELVRRMVAGLSGEIALATILVLFRAFKTLLNMENLELPAALTSLGDLATMESYAKYIGYHHLPILLSAIQGLNVDTNISSKVNFGSLVELLISILDNQKEMTDMTRLQILVGLGVIFNKYLTWNDFTNTFGEPRQSSSLSSDKLEIIIWCLYGLVLKCNSDATEYFVELLGTLSFVQASKAIKIIFTTVTDVAENSGEGDEIDISNGLDIEAELICVYKKEKTGLMMVRNKNKYAVSNLILRNMWKQRILEVMLVRRTEDDSKMNVILPLVLTYLPEDLYSSHLGTLLPNLIGTVESSDDQKVVIAVLKIVSNVVLEQSGRELLKPYLNSIMEIILKNVEDLNKQCSKGLKKQSLRCLLGMCLFDLPAVVPYKKRAIKAAHLTLSDKSRSVRLLGVSVRQAWEDLGVDLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRZ7</accession>
    <name>A0A1E3NRZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_88250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019998.1">
        <property type="nucleotide sequence ID" value="XM_019165034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRZ7"/>
    <dbReference type="GeneID" id="30181721"/>
    <dbReference type="OrthoDB" id="425925at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="200"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="568" mass="65403" checksum="298E70FB75C0C6B7" modified="2017-01-18" version="1">MNTHRRKSLIPKPSLPNSRKDTSRLSPAASPRKQDSRRASISPISFSSIPVSNSTTASSASSINSRKRSGSSSLEEYLSTLQTHITADVEAERSYLSEILLEIDSRLFHYHQLCTKLRDLQKREANLSNTIFEYEIGIPVKQSLLDQEVLKVEDDLARVEERIDLEKTAYAKVIEHKEQKLHNVLQELVNDAKRDDAETVKLRSERMKKLSDEKEELLRKIEVAKVNMDSELQQLRESFSSEKDLKIKNFKEKETLINLELQKLEEQHEKLKKEYSTLSFKLETDQSTIKSLEKKIQSKKIKIEELQYRLSDMKSLISELQLDLDNALTLFSKFESGEYKETRAKWLLAKTRLQQEKHKRLKIEIQIREISGIPNIVILDTDSSHALKNSSLNEFFKPLGYDWKSEMLTALESSLEGVSSCVYLFNEDKSSITNLRSIIVPHLEHVLKEQDRFSHYDTNFTELDTLNTISNILEDSTEFQISKFPLDVNALYVEMTNSKTLNLRKAIIFLITASVPSELKSESVQQFGKAVQCITIAENISASWSESLQLFASLKPLRLRESYSFLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS28</accession>
    <name>A0A1E3NS28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_25584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020003.1">
        <property type="nucleotide sequence ID" value="XM_019162403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS28"/>
    <dbReference type="STRING" id="763406.A0A1E3NS28"/>
    <dbReference type="GeneID" id="30179090"/>
    <dbReference type="OrthoDB" id="270284at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="19165" checksum="61B50589F19E2EEA" modified="2017-01-18" version="1" fragment="single">MAKTVLKVTQKIRKKKTLLHPNGRKAQSLARASLRDDRVNQKKLMHSMRKSEENLIVDFFQEFINSPAEIEKDKYELDDLKSLIETFINRDRDELETLKSERRADRPPSKRQQLLESRLKQENHMYETGWQVPDLRSATNVNLLRLWKGGHGGLTAIKCALVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS67</accession>
    <name>A0A1E3NS67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70524</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020044.1">
        <property type="nucleotide sequence ID" value="XM_019163664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS67"/>
    <dbReference type="STRING" id="763406.A0A1E3NS67"/>
    <dbReference type="GeneID" id="30180351"/>
    <dbReference type="OrthoDB" id="19102at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="296" mass="32787" checksum="9EA357A2E51638C3" modified="2017-01-18" version="1">MSELGIFVSSIPPVTRTITLTSLVMGGLHSLQLIPITAFTCHFPLVWQKFEVHRLLTGFLIPNPQAMQGMMEIYMMYSFSKGLEESKFKKNLPDYIYYFAIVLPIILVGSYAAMPPLYSLSPALLSALTFTWSILNYNQQVNFYFMPIKASLLPAVSLGFRLLVDGQESFILALLGFAAAYVYNCIETRSLGPLMSIITGKEPEVNPNNNRLGTVNTMAHAWFYSTGYLGAPRWLRRVLSRFTGVDYDSPTYNRRGFTAFAAKKSTSAGTGSSAAARAAGNVFFRGEGRRLGSGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSF0</accession>
    <name>A0A1E3NSF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14480</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020087.1">
        <property type="nucleotide sequence ID" value="XM_019160393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSF0"/>
    <dbReference type="GeneID" id="30177080"/>
    <dbReference type="OrthoDB" id="3269380at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001987">
        <property type="entry name" value="Transcription factor SFP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051580">
        <property type="entry name" value="ZnF-Chromatin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057:SF0">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="479"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="540"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="338"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="564" mass="64069" checksum="7EE8F4BBC725B5EC" modified="2017-01-18" version="1">MAIQQPGNGDSSQPINISFSRNNSLYQNPRLRRESIAHSQGVGGVSWGSVGISSWLRDEVFIQNHNNMHQNHHQSQNLNHSQNHNAGMQGGGNVQYQQQRNQSINLFNGNFNNNNAMDLRGDSIMMYDSSVPNSSYLADLEANYCKDYSCCGQILPTLHDLLRHYEEMHIQQDPTSIQLNGMNLNTNPNNVNHNNLNSSSGLDRMRMANNSIGAVPTNEVFLNNHLNTKQNLNNNNKKFSMNPKSNSKISSFNLNMTYDQNNNHNLQNNELHKTLSNFNFGDTNQASLNKLNSQEQESMQFDFSTNNGASYFQNDRLTKDLENQTKMFHEQIQHQLNNPQSAYDGNDHTTNNLNNNTSNNFNLNNMNNLNNTHQSNDNNSHADLNNTSNNTNTHNSHGTGIPGNITNNHQKIKNTQLSMHKGGGIRTHNDGEMDMDHDTQFQMDVDDANMDDDDMGQEVCIDDPARRLYVIERKDERPFQCPVLGCSKTYKNQNGLKYHRLHGHQNQTLRENSDGTLSVINPDSDSPYPNGMAFEKDKPYRCEVCGKRYKNLNGLKYHRGHTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSF6</accession>
    <name>A0A1E3NSF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020178.1">
        <property type="nucleotide sequence ID" value="XM_019160427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSF6"/>
    <dbReference type="GeneID" id="30177114"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="58" mass="6550" checksum="ABB2A870293F4366" modified="2017-01-18" version="1">MNLCLLFFAVLHKYTQRSLCVLKRVTSVSSSAFLLFHSCALLLSVCSSALRMIRMLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSG5</accession>
    <name>A0A1E3NSG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_57134</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020113.1">
        <property type="nucleotide sequence ID" value="XM_019163216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSG5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSG5"/>
    <dbReference type="GeneID" id="30179903"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015355">
        <property type="term" value="F:secondary active monocarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="52"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="477" mass="53130" checksum="4F6F0C2AB169A4E0" modified="2017-01-18" version="1">MEKPDLSCPSITRYLKTRFTELLPTKQSIAANRHLLNPLPGLRMIGGKQWLMILSAFIAWSWDAYDFFSISLNAKELANDFGKTVKDITWGITVVLMLRSVGGFFFGYLGDKYGRKWSLILNLMFVCVLEIGTGFIKTYEQFLGVRAVFGIMLGGVYGNAAATALDDCPLEARGFISGFLQQGYAFGYLLAVVFKRAIADNAKQRWRAMFWFGSGVCFLICVFRAMLPETKAFLKQKEIERYNIEHGIHQKSFKEKAVDSLKIYWLMILYMILLMAGFNFMSHGSQDLYPTLLSVRYNFSEDAQTVTNCVANFGAIVGGIMVGHFSNFVGRRLSIMICCVAGGALIYPWAFVDNANINAGVFFLQFFVQGSWGVVPVYLSEMAPPDFKAFVVGISYQLGNLASSASSTIETTIGEQFPMVSPTGKKIFDYATVMAIFVGCVFGYVLIVAFLGPERQLRNMDGETEEAGSTACHHTMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSH3</accession>
    <name>A0A1E3NSH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_57208</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPI-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the COPG family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020220.1">
        <property type="nucleotide sequence ID" value="XM_019163221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSH3"/>
    <dbReference type="GeneID" id="30179908"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000071">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000368">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000001">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="12">
        <location>
            <begin position="20"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="13">
        <location>
            <begin position="664"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="14">
        <location>
            <begin position="812"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ49107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="925" mass="104382" checksum="5CBA26E755EF8E4A" modified="2017-01-18" version="1">MSTHTYKKNEDGDASVLPDKMTVYQECLQACNASPIQAKKCRKLLSRLLRLFYAGETFPTVESTNLFFSISKLFHNDDPSLRQIAYLAIKELCSMSNDILMITASIMKDIQNGDAVYKPDAVRTLSRVLDGSTIHAAERLFKNCIVDSNQSISSAALVSSYHLLPVAKDVVKRWSTEVQESITANKVFPTSSYTQHEFTGYNKTPNSTFIHQYHALSLIYQFRNNDKMSLIKMIQQLTERKTLTNSLAQIQMIRFVEKLILDDPNFVHSLWPLFVNWLNNKSDMVELEAAKLILNNTSKFNSEQQMHAITTLQSLLGVPRTVTRFAAVRVLNKVAIKDADKVGVCNLELERLISDQSRSISTYAITTLLKTGNIESVDRLVQAISNFMDEISDEFKIIVIDAIRTLSLKFPAKYKSMLTFLNDVLREEGGFNFKNSIVEAIFDIIKFIPESKEIALEMLCEFIEDCEYTELSVRILHLLGNEGPKTANPSTYVRYIYNRVVLENSIVRSSAVIALSKFALINDKDLNKSIKILLKRSLQDVDDEVRDRAVLSLKLLESKDLVEAKSYLLPQFKYSLPILEQELTKYVKNPDKSSFKSSFSIDSVPKMSEEEYMAVQYKEKLTGGMDEVIVDGSDDLNSKNNESNDDVKGLDKISDVTKYTLLQQQYQNELSALPEIGDYGTLLHSSAPVELTEKETEFVVTAIKHIFAKHVVVEFSIENTLDDIILENVNVVTQLDTEEYTEELTLPIELLNPHSKGKAYSSFTRPEDDDEKFMLTTMNNTLAYLTKDVDAEADEEGFPDEYQIEDLSLTPGDFVIPTYISNFTNVWDELTSEESAVYNLGDEESVDLQQVVSKLIVTMSMMPIEGTEIVTSGKNHSLKLFGKSISGGRLAAIVKFAVSSKGVMMKNVVKGEEAELVELVANYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSK2</accession>
    <name>A0A1E3NSK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_90977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020165.1">
        <property type="nucleotide sequence ID" value="XM_019165066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSK2"/>
    <dbReference type="GeneID" id="30181753"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="160" mass="18160" checksum="73336031E630B609" modified="2017-01-18" version="1">MSDPVCNACSDKTSRSHQQKPTHCRDIESSSYQTLHPTPNTLHLPTHIHAFTALNLTKTKPLKTMITVHEQQMSKRADSQVLQDAQDPCSGCDVADMHSERVFNQIQRFYREKETPVRNSNAIRYTSAAQVERLKAYSNNDFAASRIRDLNNGYLPSLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT60</accession>
    <name>A0A1E3NT60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14669</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020309.1">
        <property type="nucleotide sequence ID" value="XM_019160480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT60"/>
    <dbReference type="STRING" id="763406.A0A1E3NT60"/>
    <dbReference type="GeneID" id="30177167"/>
    <dbReference type="OrthoDB" id="28092at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="779"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="11">
        <location>
            <begin position="560"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="809" mass="91344" checksum="520D7F5EA0842116" modified="2017-01-18" version="1">MYRYQSETVPIRPNSGLVEVSNEMIISFINYEYEPGRKVKSKLMLWNVSKSFGTIEKELDFTSSIVSVLLKDDHIYVVDERHILDIDITLDYNITTIYTAEQPIEDAKIFHSCLDGLTYAVLEISGKTFFSSLSDFVSRPFNGCHVKQLRFQNSSNNLIICSNSDVYAFDSFGYEKLKSGKNMINEKLSAESLSADQIKSFEIVKDHILIESNNEIALFDLESLSLSANAKSLLSNPLSDSIHYSFHVSKNSTEINLLVVSLSKVVDYYRNGELLWSNDQSFVNIKDFVIVGSELKSSLTVDELIFETSSNILLSYLRRIRHNYESLFGKLNSALDEAHRFGMSKYLIALSENGKIGVFSMYKNENSKSQLKKIFTPQIHFSHLYEIERKVYALHNTTIYEVDINLGKITKKSETFVNSHFKILQNQDLKAEFVQTDAHEFYTISISHISNSIQGHFWNERQVDSWNFSPEGETILSLVQRSYGNVDVAQNAIVLPDRSSLYKYLIPNVGVLITHKEGESSDCVVFYLINLITGQVYGRLEKKVSKSINTAYDVHVAFEENFIIFTVPDKNTIIDTQICSIDLFEVLKPDEKLSGKNAVISAFDEPILPMFASQCFVLPGTSVRGLSISRTRHNIATKDIILLTNMGRVVAFPKMIVDGRRNGIIGDFKLKNDVESSVISLDVAKVSPFLQRIGSSKYASSIASKFPYDPIINVNPQTILTHHRKLVLNNNPGSTFLFTEPTELESTTYVVSIDGDIFVTFLRPSSSFDRLTSSFNTKVVIATIVVLLIGIASVGPKVHRNKILGRWVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTI0</accession>
    <name>A0A1E3NTI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-rRNA-processing protein ESF2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">18S rRNA factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pre-rRNA-processing protein esf2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_25475</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020568.1">
        <property type="nucleotide sequence ID" value="XM_019162394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTI0"/>
    <dbReference type="GeneID" id="30179081"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="81"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="226"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="283" mass="32165" checksum="A5E6799CE0372DB6" modified="2017-01-18" version="1" fragment="single">DSDNETGDDVDEDDEDQYYGFVGDEEEDVEAEKAEAKTSVEKTVEVLEGQLLGEASAKSKKVKRLTPKQLENEEKRVKRTGVVYLSSLPPYMKPQKLRQVMTRFGEVGRIFLKPEDPKVYKSRIKSGGNKKKKFDEGWVEFVSKNDAKLAASTLNGNVLGGKKRSFYHDDVMNVKYLKGFKWFDLTNALNREVEVRDSKKQAELSQANKLNKAYVRNVETSKTINRIKESKKRKLPDGAEAEAPHIRREFKQRHVATNRAGADDSIKNKDTNSGLDSVLNKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTV2</accession>
    <name>A0A1E3NTV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">DNA repair and recombination protein RAD54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_71058</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020625.1">
        <property type="nucleotide sequence ID" value="XM_019163812.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NTV2"/>
    <dbReference type="GeneID" id="30180499"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016817">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000010">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000332">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013967">
        <property type="entry name" value="Rad54_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08658">
        <property type="entry name" value="Rad54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="456"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="492"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="521"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="753"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1087"/>
            <end position="1240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ49512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1326" mass="148415" checksum="F690852BBD822C06" modified="2017-01-18" version="1">MSQITGNIPIISCEQVYLLPNQQQCQFVQAQCADHPGFLNSFTMYYCMSQSDSVRSWVGIPLMVLLLLVLFASIGLVAGNCLVPNLNAVASHLKIPENVSGLTLLAFANGSPDIISTYTSFVTGNTSLAFGEIIGAAYFTNSVVIGTIFIINPFDLVPGVSTGPNTFISLQDNANTGSNSNGNNNSGNNKDESAEQRLISLNAKGTYLRDVSFFTVSALILLYCVRDGVLTRIEMLTLVSIYITYVLLIILWQWYYKKELNKIRLESHARSLYNQNSLPFPFDENIQMQDSYNYNPQIIRNLEFETILRGLTSRRRIGFYIEQPGGTYRDSSDGSNTNGDISQGGQLQAEIIEPPKRSLPEKIFDYAALPFVKLFRHTIPIMTTNDYEGDYKPALAQLFELLTSLLTSPFIIVCTLFPTTSGLTKFFMLFPTITLTYFAYHSLVRSSNPSTPFKCVISIMGVAASIAWISIIASEIISLLTLVSSLTHIRPSAMGITIFALGNSVGDLISCIVITRMGYPLMALAACIGGPLLNILLGLGMSGLLVGADYIEIPATTRNSTTGAGTQGENGRLMRKARLQAPKSYAEGGNSSLKDDSPSDMLWFDENSYGANNTMKKKNALHPRRVLGYVDENRMILSRKFVPPLKSKTDDWSLQARVPPPLGNRKKVFFPPKPLYDPFGELAIVLYDPTRPASRFKMHNKRSLNEMLGISSNSQDPTASFPNVPVVIDPKLAKILRPHQREGVIFLYRCTSGLVDARAKGCIMADEMGLGKTLQCLTLMWTLLKQGPRGKKTIEKCIVVCPSSLVRNWANEIDKWLGKGSLNSLAIDGKSAKGSSIGESIQNWADAQGNGVVSPVLIISYETLRRNIDNLQNCEVGLVLADEGHRLKNGDSLTFNALNSLNCQRRVILSGTPIQNDLSEYFSLLNFANPGLLGNRLEFRKNYELDILRGRDSLATDKEKEKGDEKLKELTEFVQKFIIRRTNDILSKYLPVKYEYVIFCGLSDMQKTLYNHFITSPEIKKLLKGEHSQPLKAIGLLKKLCNHPNLLNLPEDIDGCEDLIPEDYKDDRNSTGRNREVQTCHSGKFEMLERFLFKIRMETDDKIVVISNYTQTLDLVEKLCRQHKYGALRLDGTMNINKRQKLVDRFNNPEGKEFVFLLSSKAGGCGINLIGANRLILMDPDWNPASDQQALARVWRDGQKKNCFIYRFISTGTIEEKIFQRQSAKLQLSSCVVDSVDDVERYFSSDNLKQLFQFNTNTICETHDTYKCKRCDASGKQKIKSQVMLYGDATTWNHINHSMLDKNEDFLIQNESQFETVDFAFQYISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTX4</accession>
    <name>A0A1E3NTX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C-8 sterol isomerase</fullName>
            <ecNumber evidence="8">5.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Delta-8--delta-7 sterol isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70698</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Steroid metabolism; ergosterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ERG2 family.</text>
    </comment>
    <dbReference type="EC" id="5.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020241.1">
        <property type="nucleotide sequence ID" value="XM_019163710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTX4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTX4"/>
    <dbReference type="GeneID" id="30180397"/>
    <dbReference type="OrthoDB" id="347124at2759"/>
    <dbReference type="UniPathway" id="UPA00768"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000247">
        <property type="term" value="F:C-8 sterol isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006716">
        <property type="entry name" value="ERG2_sigma1_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868">
        <property type="entry name" value="SIGMA 1-TYPE OPIOID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868:SF1">
        <property type="entry name" value="SIGMA NON-OPIOID INTRACELLULAR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04622">
        <property type="entry name" value="ERG2_Sigma1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="202" mass="22962" checksum="5B93AF3A8FD89104" modified="2017-01-18" version="1">MNFLYYTWLPTNYIFDKEELQKLVKDTLKVAPKDDINAIFITLQEKLEEKYGSAYINKLNMDDWVFNNAGGAMGQMFILHASISEYLIFFGTAVGTEGHTGIHFADDYFTILKGEQTAAYANQFSKSVYLPGDQNFMPMGKFRQYSMPAGSFALELAQGWIPAMLPFGFLDTFASTLDFVTLSRTIYFTGKDMIKNLLQGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUZ8</accession>
    <name>A0A1E3NUZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_102618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020601.1">
        <property type="nucleotide sequence ID" value="XM_019159653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUZ8"/>
    <dbReference type="GeneID" id="30176340"/>
    <dbReference type="OrthoDB" id="3997609at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="309" mass="34720" checksum="B7BC82844B919314" modified="2017-01-18" version="1">MQDMLVKPGIQELLKYNDMLLYQQQVKILSSEFSNDLSILLYVLYYILFIYDESVLCFFLRCFMQLVLSYVMSPSNGSDEMIPGLSRLNISRLSGVTGTNSTTAGSTVANNNGNEPGRSGDANNELNVDTIASTESFDDIEALFSRWTVADKVKFVQKILLCLAVGWGLMIVYQLAFCLDDTYGAISYSVFGSLNKSIRQSAYSNKHYANLDQWSSENAFTTGSVLLNMIGETKLRSKFMKFFTIIMLDCFIITIQLIEVLLNYIVGFGIVERITEGANSTNDPQREYDGRQGRTTILKLNPFEAFGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND66</accession>
    <name>A0A1E3ND66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_18540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015175.1">
        <property type="nucleotide sequence ID" value="XM_019162049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND66"/>
    <dbReference type="STRING" id="763406.A0A1E3ND66"/>
    <dbReference type="GeneID" id="30178736"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="58902" checksum="C678CCD2B789C7F8" modified="2017-01-18" version="1">MTSASSSHEQNAANEKENVDVGVSSVDNFTALDTVISPSGKVIHIAHDDAHLDEALEFARAHGHVDISPEEDAKVRRKLDMIIMPLFCFLYMNQFMDKTGISFASIMGIQKDYKMVGQMYSWTNSGFYLGYVFGSPFAAIVLQKVPTVRFVSFTIILWGTIQCLHCTPKTYAAFMFLRVFLGFLESFVAPIFVIILNQYYRHSEHFGRTGVFYGFNGLGTIFLSAVSSALYKHSASYSISGYKILFLVIGLMTIINGFLILLIMPNTPTEAKFLTEREKEVIIERIRHNNQGFGNKKFKWEQAVEVCKDPRTLIYFLLSLSVAIPNGGISGFGTIILKSFGYTTLKSLLMKMPVGAFELFGLAILPIVSRFVKSRMAIAIFYLCVVLAMCCMLAFSKNTNAALVGYWIYGISPVGIVLITSCVTSNTAGFTKKLLTNAITLIGYSAGNVIGPQTFRSTEAPHYNNAKSGAVGCYCAAIFLVGVLTAYNIWENKRRDKAREAEGDKYVVPDNLEFADLTDFQNPEFRYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND80</accession>
    <name>A0A1E3ND80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_74923</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015198.1">
        <property type="nucleotide sequence ID" value="XM_019164915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND80"/>
    <dbReference type="STRING" id="763406.A0A1E3ND80"/>
    <dbReference type="GeneID" id="30181602"/>
    <dbReference type="OrthoDB" id="4940293at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="154"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="217"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="375" mass="41354" checksum="5BE63B5A7EABDF09" modified="2017-01-18" version="1">MEYNTDSQLKLQSVNENEKGLGATGPALDINLALSPGVGSGDSGNLQLFGLMDYGTPDPLSFGALVQYQQQQQQQQQEEKSHDGSTSSVFSDSHNHNYSIGHADGNRRSASGNAHSDGSDTNMNDAGQPTSNTGYSSPDGAATVKNGTAGPSEEELYQRRKAQNRAAQRAFRERKEGKIKELSSRLQDAEVSRERLEKQLSELRERNKRLNMENQMLQKQQQFQQQSQAFQPVQDDAYTSPGAREGGGGGVGGNDHGLADGLDIQDILSFKFPNTSKYEFIEGTIDWSSHGQENESGYHASKIGESYRHDSNKLLTISAVWDYLVEFANLNEEYTLDIPGIMNELRGKEVCHGFGPAYALQLVNDIVCRHIRNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDT3</accession>
    <name>A0A1E3NDT3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015404.1">
        <property type="nucleotide sequence ID" value="XM_019161915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDT3"/>
    <dbReference type="STRING" id="763406.A0A1E3NDT3"/>
    <dbReference type="GeneID" id="30178602"/>
    <dbReference type="OrthoDB" id="5396786at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040347">
        <property type="entry name" value="YBP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020:SF1">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="696" mass="79412" checksum="A5F86C44B3B71DF7" modified="2017-01-18" version="1">MSDTEVESLNTVSPLTFKEIVANIKDCAAETAQTQDFIGFSTVLDLYLHDWNVYSVEERLALMELLCQILDDDKELLAEVAWDLPPLLLPFLDCEWPVRFALKDTVEVPLVWKAFNLLATYGNPKELLLTCCEQLGQLADPVEDAATIDEAEIIEIETNMDDWYKTQYNDLSYLIQDYVTDWSNRCVIIKFHTLFQCIKFCVQRVKTLYPSKFLSMAVSSILNFFNSAEHISGVISVQRSLYIFLRDYIPPEIPDDTLTSGENTPEELARIFDDECYLQRKLIRLLFDSIVDKMARDHHRVFIAKLLPKLGFPKMASEELFFELINRLLSLSLSLDIDLSGSLAKEIKDAAFLFDGSDNKVESSEDILSLAVSAYNNSSFRSKEPKALPTSTTSLGFLYMYAKYIENWNISIPESIKVLDLIKVQLKMFIPYIVNSKLTDLTSVGYFMVLTIIKIEKSKVIASKEEFEIKKVNLLIRTYLQNISSIICRSDSLIMAKLYSKFMKKFLQHLPEDISYSYILDTLTNCPFDENVLSTLTIFKNLISMSKFDDENLTADLSELSLDGKESSKKSALPPPLPQRSGKGFKTSFINFTKLRQDDFINLVNEWTAATFDSNESNLDVMKSNRLLSYLNFINSVRFEDKRKLLSVLNNIEKLASQVQSKVNGETDNSIPVIIDLIKFAVNKGNKIYGDGLKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDX8</accession>
    <name>A0A1E3NDX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">AIM41</name>
        <name evidence="4" type="ORF">PICMEDRAFT_21451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the AIM41 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015442.1">
        <property type="nucleotide sequence ID" value="XM_019162204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDX8"/>
    <dbReference type="GeneID" id="30178891"/>
    <dbReference type="OrthoDB" id="538640at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="entry name" value="Uncharacterised protein YqeY/AIM41 PF09424, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="156" mass="17715" checksum="AD5F6032ADDB48E6" modified="2017-01-18" version="1" fragment="single">SAGPVYDGFLGSLRADLKTCIRTKAPALEKTTVRGILSEMKNLEIENHGSVVDEFKIYDHLNKLVKQRKETASEYLKPDQPERFKELAQKELDEAKIINKYLTALPVASEDEIVAKLTELMKTENITDKRKLFQKIPWGKINKEWRASKGAVSNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEB1</accession>
    <name>A0A1E3NEB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxin-19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015583.1">
        <property type="nucleotide sequence ID" value="XM_019161970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NEB1"/>
    <dbReference type="GeneID" id="30178657"/>
    <dbReference type="OrthoDB" id="21292at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="35624" checksum="6528F0759A3CD0EB" modified="2017-01-18" version="1">MADEFDDLDDYLDEFDEEILSQEPGATVIDKKGGAESNLKLSNNDKAPAEEKESADIKEFAAAAGLDPQFSKQLEDFMSMLDPQGTGNELNSLLSEPDIGEKGKTTVNGKENGFQDTVNETINRLKTSSQQVEDESTQKMDKNEELLTTLLNSMDIGGDESLEGFDELKDLLSENNGGAMGAGDGDVDKLTNVIMKMLNRLTSKEMMYDSVNTAVTNYHEYFKDLKNSSKPSEDNEDYKRYVMQLKHLENVKNKFDDSSYNEEDPETRDYIDQEMENFNKILPPPPGVIQDNLADMGLDNVKWSDKEIPTDLEGCVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEB3</accession>
    <name>A0A1E3NEB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C-22 sterol desaturase ERG5</fullName>
            <ecNumber evidence="6">1.14.19.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome P450 61</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ergosterol biosynthetic protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_18385</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.41" evidence="6"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015600.1">
        <property type="nucleotide sequence ID" value="XM_019161981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEB3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEB3"/>
    <dbReference type="GeneID" id="30178668"/>
    <dbReference type="OrthoDB" id="1372046at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000249">
        <property type="term" value="F:C-22 sterol desaturase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11082">
        <property type="entry name" value="CYP61_CYP710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000021">
        <property type="entry name" value="Cytochrome P450 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF228">
        <property type="entry name" value="C-22 STEROL DESATURASE ERG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="5 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="527" mass="59963" checksum="CDE6FF81BD39B0A8" modified="2017-01-18" version="1">MEALKVDAKDTFEAVSNSTSTLGEKTGLMSGLGSMSWLQIGISLLVVAISYDQISYQLNKGSIAGPRFKMWPFIGPFLESLDPKFEEYVAKWNSGPLSCVSIFHKFVVIASDTTLARKIFQSPSFVKPCVVDVAVKILRPTNWVFLDGKAHVDYRKGLNPLFTKKALEDYIPEQEKVMDKYLERFVEVSDKGPQIFFHEFREYMCAISLRTFCGDYITKEQIQLIADNYYKVTAALELVNFPIIIPFTKTWYGKVIADKSMKIFEACAQLSKDAILKGKKPNCVMDAWILSMYESRGDIVNENSDSKTKVRLFSNREISEAVFTFLFASQDASSSLCCWLFQIVADRPEVAQKIREEQLRIRNGDPNVKLTPELIDKMEYTNMVVRETLRIRPPVIMVPYVAKKAYPVTDSYTVPKGAMIIPSVYPSLHDPEVYENPEEFIPERWLPGAKAWEGKKNWLVFGTGTHVCLGQIYVMMSFTALIGKACMFTDFKHTVTPLSEQVKVFATIFPMDDLILEFRKRDPTTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEB4</accession>
    <name>A0A1E3NEB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_13659</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015575.1">
        <property type="nucleotide sequence ID" value="XM_019160095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEB4"/>
    <dbReference type="STRING" id="763406.A0A1E3NEB4"/>
    <dbReference type="GeneID" id="30176782"/>
    <dbReference type="OrthoDB" id="3997648at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09496">
        <property type="entry name" value="CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="337" mass="38705" checksum="D5379C0F4157B601" modified="2017-01-18" version="1">MSTLDEVRNEIRELELEIEELEQEGGEDESFDGEPHNDDISNYATLDQLGKMDELLYDILNDKNDDLDMGEIVDGRRILRSKELQALHGDEIPKIQIENVYRFNGITMFPISNDPERIFIGIRFDVFNSYLKKFTTCHYIILKQLDIEASEKEQQLQISPETKAGNFNWRWEVFQTTVPKHVPIGDVARDYLSVEHTREPASDAAPVPSFNRMNKFAMHIYEHLSLLEERKAFAYKLQGQYASTKRVLVKFDPAVCRIEITLFDKKRLTTTKLVVDSDSTATRFFLNVKGSGGAENESRNVAHTYWSSACGAVNVLPDMRSFAVAVQKSIDTFLLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEL2</accession>
    <name>A0A1E3NEL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_24135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015649.1">
        <property type="nucleotide sequence ID" value="XM_019162336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEL2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEL2"/>
    <dbReference type="GeneID" id="30179023"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018484">
        <property type="term" value="F:4-hydroxybenzaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047770">
        <property type="term" value="F:carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046185">
        <property type="term" value="P:aldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="3"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="418" mass="45908" checksum="DE0A00E71A04EE22" modified="2017-01-18" version="1" fragment="single">EPIANAVKKDFNKPRMEVYLTEFGQAVAELNNVLANLDSVLKPTSPSSLPINFSTLTIQIEKIALGTVLIISPFNYPLILAISPLIGAIAGGNNVILKLPFDQLPNFSTVMKNVIEAALPKDQVAVVNGGIPESEYLLNECKFDKIFFTGSTNVGKIVYEAASKNLTPVVLELGGKSPAFVTNNVDKKSIRTLLDRLLWGKFTNSGQTCVAPDYLLVEDSVYDDVLETLLQSYQSTYAELDETSDFTHIINKKGFLRLDGLIKQTRGKVIAGGKTFAGSLFIEPTVVADVSWDDPLMSNEIFGPILPIMRYSGSFQKIVQKVATTHDCPLTAYLFSFSDQDLVTLKKYLRSGSISVNETLMSAGCFISPFGGIGSSGFGNYHSKWTVDSFTHERAVLKQPLWAEILLKARYFPYTQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEP0</accession>
    <name>A0A1E3NEP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_23562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015730.1">
        <property type="nucleotide sequence ID" value="XM_019162313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEP0"/>
    <dbReference type="STRING" id="763406.A0A1E3NEP0"/>
    <dbReference type="GeneID" id="30179000"/>
    <dbReference type="OrthoDB" id="110024at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056453">
        <property type="entry name" value="HTH_DNAJC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23302">
        <property type="entry name" value="HTH_DNAJC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="204" mass="24033" checksum="DDF89A74EFC5D904" modified="2017-01-18" version="1" fragment="single">INPYEVLGVDPACEGKDIRRAYHKLCLKYHPDKNTGFRKEFDRVQVSYMVLSDSKKKARYDRTGVINLGSGADDAEAEDFDWTDYFRTQFDAISKEMIDKDREEYQGSAEEREDIKKELVALKGDMSKLFEVVIHLEFTIEQEKRVFEICQELIDEGEISDKSIPKWNTYVLKRDQNVKKMERKRCREEKAAEKVEKKRKETGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NES0</accession>
    <name>A0A1E3NES0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_74345</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015743.1">
        <property type="nucleotide sequence ID" value="XM_019164743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NES0"/>
    <dbReference type="STRING" id="763406.A0A1E3NES0"/>
    <dbReference type="GeneID" id="30181430"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="21"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="306" mass="33353" checksum="2BBF5591E856B40D" modified="2017-01-18" version="1">MVEQSFAVNLSHIIPHGKLLEGITYDERTDTLYYIDIPAGSVFVSPNAANTPLGVPNSYVVSASVGVIGLTSDPNKLICGVEDGVSILDLAMGEVENVKAFPNGNIAEGTQLRSNDGSVGPDGSFWVGTMDDTEEKSLGSMWLLKNKDSELKELWSGAGIPNGINWDSSRKIVYWTDSLKETIYKYNYDPETAEIDLDSKESWFSGVSTPDGSCIDKDGNLYVALWGSNKVVRVTPECKIDMEWKFPSKNMTCCAFGDKDFKTLYVTSADLGEGDEPDANDNGAAVYRIELGDLNIQGVPKNKFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEW5</accession>
    <name>A0A1E3NEW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_22082</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015808.1">
        <property type="nucleotide sequence ID" value="XM_019162234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEW5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEW5"/>
    <dbReference type="GeneID" id="30178921"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000007">
        <property type="entry name" value="Basic krueppel-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="351"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="389" mass="45195" checksum="C6143AFCB9FB6B37" modified="2017-01-18" version="1" fragment="single">SPRSASSSRASSPRPKNYLCDFPGCLKAYSRPSLLEQHIRTHYGYRPFKCRVPTCEESFMRKDHLERHMLKHVGEEDKPFHCSVCGRGVNSKQHLKRHEKTHYKSFVCTYDGCSESFHKHQSLKAHIRAVHEGLSGHVCRVCGKQFNRPSRLVDHMEKHHSETAKLMCDFPGCYQTFRVWSALQLHVKTEHPRLECEICGKKCVGATGLANHMKIHDENTVIKLWKCVECGEKFHKKEDTVRHFAERHPLLELPEALQYNVKDEKERTMKVANSQKHKLDDGIEEALRLSDSESEAEAEKELNHEDDKSLSDCGFIVSSPTQNGFKRHQTNRVPSSPDVLDLIIDNVDIRLHCPYTNCHRLFRKDYDLQRHLTWHEKQDRMLEKKVGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFK8</accession>
    <name>A0A1E3NFK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_37266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015471.1">
        <property type="nucleotide sequence ID" value="XM_019163069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFK8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFK8"/>
    <dbReference type="GeneID" id="30179756"/>
    <dbReference type="OrthoDB" id="2100652at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014807">
        <property type="entry name" value="Coa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042432">
        <property type="entry name" value="Coa1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28523">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28523:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08695">
        <property type="entry name" value="Coa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="143" mass="16031" checksum="E316B54AA4B1CFD7" modified="2017-01-18" version="1">MTINTELPDDPRPQQRMQAILTFLVFAAGMTVACLGIFNYEKVSSPIMNATMYFLRRSEHARELLGSKITYDGLFPWIYGEVNTMKGMVDCTTSICGDKSSALMVLKAEKKADDKFTIIQWLLIGENGEVVDLTNDASVDLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFL0</accession>
    <name>A0A1E3NFL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73714</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer with PAM16. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016022.1">
        <property type="nucleotide sequence ID" value="XM_019164561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFL0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFL0"/>
    <dbReference type="GeneID" id="30181248"/>
    <dbReference type="OrthoDB" id="240298at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF29">
        <property type="entry name" value="MITOCHONDRIAL DNAJ HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133333" description="J domain-containing protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="84"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="143" mass="15975" checksum="FB352438CDA41BF2" modified="2017-01-18" version="1">MQLPIIIGIGVTVAALTVKATANAVARCHRLTPGEIARLNGLVVGGAAGQFRVWPANSPYWRRFHDLDGQMPYGGFDGKMGKEEALEILGFGREDVLRRAVTWDEIRRRHRQLMMGNHPDKGGSKYLAMKINAAKQMLEKDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG28</accession>
    <name>A0A1E3NG28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SRP9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015631.1">
        <property type="nucleotide sequence ID" value="XM_019164857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG28"/>
    <dbReference type="STRING" id="763406.A0A1E3NG28"/>
    <dbReference type="GeneID" id="30181544"/>
    <dbReference type="OrthoDB" id="5419752at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039432">
        <property type="entry name" value="SRP9_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05486">
        <property type="entry name" value="SRP9-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP9" evidence="2">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="138" mass="15508" checksum="6A6964D0CFCF4F46" modified="2017-01-18" version="1">MKVKQIDTYITSTHSLLKSNPSGTTISITYTHVKKDDDKIRSIIKFKTYDSKNGICHVFKTHKVKEFSKLFNALGPRGCNVDGQDVDGLSLLLSNTDKDLVIKEVSECVPSNEKIKVEAETTPEPIQKKKKNKKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG35</accession>
    <name>A0A1E3NG35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Survival factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17591</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016207.1">
        <property type="nucleotide sequence ID" value="XM_019161658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG35"/>
    <dbReference type="GeneID" id="30178345"/>
    <dbReference type="OrthoDB" id="2590239at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051385">
        <property type="entry name" value="Ceramide-binding_SVF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033394">
        <property type="entry name" value="Svf1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013931">
        <property type="entry name" value="Svf1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107:SF1">
        <property type="entry name" value="CERAMIDE-BINDING PROTEIN SVF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107">
        <property type="entry name" value="SVF1-LIKE PROTEIN YDR222W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08622">
        <property type="entry name" value="Svf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17187">
        <property type="entry name" value="Svf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159245">
        <property type="entry name" value="AttH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Svf1-like N-terminal" evidence="4">
        <location>
            <begin position="57"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Svf1-like C-terminal" evidence="5">
        <location>
            <begin position="238"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="45039" checksum="EB7CE8A1FD68490E" modified="2017-01-18" version="1">MWKLVQSGLSVVAGTAEPEYGPDSIHPVASDLKEGEPLYSDLTIEDLKYVSPNHTNVETQTFYFEDTKHAGFAQVIHSNVIGLHTTAQFTFKIFEKETPQDFIWTSTKLENFSIENGTDFKADNLSIVLNKDKINTYHIDSSVNKLTEVHLTVKRIGSSIKFGKDGTTYYGTEVDNPWGSMRHVFWPRCNVDGNIILREVKPEQDLDNLEPEEIEYVVKENVTIKDGLTMYVMALQGMKPHHAAATWDFLNYQSDDYSVVLMEFTTPPAYNKTKVSISMVVDKEGKIILGTLDNDTKHLETKVDETSGWDIPSKISYTLKDPEDDLNVEVVGELKNLCERVDVMAEIPQFVKSIVSGVAGTKPYIYQFSNTMKMTITKNGKVETTDSGYAYSESTFITAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG95</accession>
    <name>A0A1E3NG95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016270.1">
        <property type="nucleotide sequence ID" value="XM_019161678.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NG95"/>
    <dbReference type="GeneID" id="30178365"/>
    <dbReference type="OrthoDB" id="1414216at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028275">
        <property type="entry name" value="CLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF6">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clu" evidence="4">
        <location>
            <begin position="330"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="972"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1276"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="731"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1330" mass="149426" checksum="89987493FEAAAEB0" modified="2017-01-18" version="1">MSEEQQEQEQQQQTVDEKIVSLKIQLPEADESLEIPCSVEDSLFDIVETLKVLPSTREYTSYQLKIDNKAYGEETIISELVKDSEETIYISLVFSPYNEITARKHVIAAREYAGLESKDDAFAEISGVCSGASTYEDLGLEESTPAAPKTDKEAEDEKGNVEEERQGNEIIKDFTEEQKSQLSELVNEICDLKPDLKVVAAKPNLKTSPALKSLFFSQWCPSDLRRKVAGDLFYLQAQTLEGENFNITAHVSGFFVNNSSNTKFDGSVHSFNSTRVVKNYSLISLLKTLSPMFEKQIEQNNLELSKHAIETYIVPNTTTVTSPWLVNSLESSTPDLGRSQYNLLHGGIDGADLQVDWNKDYQLLRDIPKTNLTQRYSREQSLISTSSSFTSAATKSAMAVVRGEIDPINPEEESEYHIFLRNGIFYSKAIDSIGQFKSTGGDEAARSTVAKDVAALRYLNRYDIGGVHSLLTTIVDYLGHRIVCQAPVPGIFQDSEDENAEPVQSVKYGFIDDHSDVVTDETFAEQFKEVGEAFHLKPHKIWNLDGSKVVDVVTSGYTKGTKGSDNKSYIIDLFRTTPLDIEFIDENYDVSKKDSYPHRETLLRHEAINEWIKRETLLAVKKETERLEKEGKSNSEIKETIGVDNSIFLLNPDAFSLTPAPTPELAEELKSDEGKVREVSKFVNQVLVPEFIKEMEKAQVYNAIDGSHLSAILHEYGINIRYLGKIANLAAERKEAYLKEQESKAAEIEKINERALEKDEKEIAEKKAKLEARLKARKEAAEKGEPIPDFKEEEEKEAKEAEAAEKELSTELNTIPVPALLDSLYVLSINEMIARAAKHFLRKALEFVPLPLAPYVISHVHNCLLSSKANPNPEAPKLDSFLAEVYNDVDLSILKQNSKSIAESISQEVHIRYRFDLPTNWSDLILVEQLMRSIALKFGIQWRERAYAFTKEALSSQIENLTNEFNAAAATSSHTSKYDDKHSKKKGRKSSPKAEIPTEVTVISTTFVPEDIVCIAPVVKNSIFESTSVPDAWETGVLKLSSQDEAQVQEGSIFINQAVQFCERLYGPVHNITATYLTKLGNLYASSGAEYDAVDLFKKSFQIFERCAGIDSFQAALALSQLANAYVSNKQIINAVKIYKRLIQYWILAFDEYHPNVITILTSVAVILMKLEMTSDAIKVFTKTLKLSDKLNGELSQQSAFFRYQLSQLLLSDKKFQEAFDCAEKAFEGFKATLGLEDKSTVDAKKLAVGLKNYNEYMKYQAKNLQEKEQEARKLEQQQHIKAKQAQKAKQVNPDPEIANKSIDDIVAFISGSNNASKKKKSNKKKTSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGI2</accession>
    <name>A0A1E3NGI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="5 17">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_74022</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 17">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="5 17"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016364.1">
        <property type="nucleotide sequence ID" value="XM_019164649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGI2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGI2"/>
    <dbReference type="GeneID" id="30181336"/>
    <dbReference type="OrthoDB" id="1530at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000048">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ45251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="345" mass="37638" checksum="35BCF3F0A52848FC" modified="2017-01-18" version="1">MVHSAEYLPGQPTDISSVLQGGYNNPLLRAWQHERQLQKNMFIFPLFISDDPEDETDIPSLPNIKRFGVKKLIPYIDTLVKKGLKSVILFGVLLDQASKDAEGSPADDPDGPVIQAIKALKTNFPDLYVMCDVCLCEYTSHGHCGVLYEDGSINTELSVRRIAAVAVNYAKAGADSIAPSDMIDGRIKAIKRGLIDAGLAHKIFLMSYAAKFSGSLYGPFRDAAGSCPSHGDRKCYQLPSGGAGLARRALIRDMNEGTDGVIVKPSTFYLDVVKDAATICSDYPICCYHVSGEYAMLHAAAEKGVVDLKSIAFEAHHGFLRAGARLIISYFTPEFLEWLDEPVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGJ6</accession>
    <name>A0A1E3NGJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016388.1">
        <property type="nucleotide sequence ID" value="XM_019161728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGJ6"/>
    <dbReference type="GeneID" id="30178415"/>
    <dbReference type="OrthoDB" id="4138121at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020301">
        <property type="entry name" value="Mrx7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10906">
        <property type="entry name" value="Mrx7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="84" mass="10212" checksum="993DFEACDB7FA1BC" modified="2017-01-18" version="1">MLEEWLYRRLITSPRFHYYVRVFHAMVNNLPPPQPPRHMRGPQLKTGKGKVYNATTFKPTTAQKWNAYLKIWKEEMKDSFLFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGQ4</accession>
    <name>A0A1E3NGQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_60394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015861.1">
        <property type="nucleotide sequence ID" value="XM_019163433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGQ4"/>
    <dbReference type="GeneID" id="30180120"/>
    <dbReference type="OrthoDB" id="10351620at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="88" mass="9695" checksum="3C0B1DECCE17A2A6" modified="2017-01-18" version="1">MSAEETVNQATQSAEAAVDNVQKTEQKESATFVDKVEDAAKKASEEIKKEADAVREEAKKTSAKVNSELEKSSNPLKKLGNYFKKVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGR2</accession>
    <name>A0A1E3NGR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="7">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74069</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="7">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="7"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016415.1">
        <property type="nucleotide sequence ID" value="XM_019164667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGR2"/>
    <dbReference type="GeneID" id="30181354"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902693">
        <property type="term" value="C:superoxide dismutase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016670">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901856">
        <property type="term" value="P:negative regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 7" id="KW-0186">Copper</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="154" mass="15797" checksum="D90071910BF5CC81" modified="2017-01-18" version="1">MVKAVAVLRGDSNVKGVVSFEQSSASEPLSISWEIEGNDANAERGFHIHQFGDNTNGCTSAGPHFNPFGKTHGAPEDETRHVGDLGNIKTNGSGVAKGTKTDKLATLFGENSILGRTVVVHAGQDDLGKGGHADSLKTGNAGGRPACGVIGFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGV2</accession>
    <name>A0A1E3NGV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016480.1">
        <property type="nucleotide sequence ID" value="XM_019160010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGV2"/>
    <dbReference type="GeneID" id="30176697"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133369" evidence="1">
        <location>
            <begin position="24"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="260" mass="27631" checksum="3F2191EC914609F2" modified="2017-01-18" version="1">MGQFPRPTVVLVLVRFFFPLLLPLEQLTLAPKHPYAPLAANPESAPTADAAARGLEHEQFLHTKVAFGTDDAAALTAFPPAAAAAAAVAAAVAVAVATDASAVCLSPRGPISSHSAGVSSVAIDVANAAEITRRTSCHGREHLRNISLEETPPFAGNQLHRLRSVLLPDGRAFGLYVQTAQIVHRATSSTLRDNIDYRRLVSSAGLFCTFQLVKPLHLAQKILRIPNFSPAFFGGISRATSASAARDIFFHCNLVDLVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH24</accession>
    <name>A0A1E3NH24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">DNA damage-binding protein CMR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_74191</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-binding protein that binds to both single- and double-stranded DNA. Binds preferentially to UV-damaged DNA. May be involved in DNA-metabolic processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat DDB2/WDR76 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016544.1">
        <property type="nucleotide sequence ID" value="XM_019164702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH24"/>
    <dbReference type="STRING" id="763406.A0A1E3NH24"/>
    <dbReference type="GeneID" id="30181389"/>
    <dbReference type="OrthoDB" id="9890280at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000001">
        <property type="term" value="P:regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050853">
        <property type="entry name" value="WD_repeat_DNA-damage-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="311"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="514" mass="58483" checksum="447C5A009FC9AC8A" modified="2017-01-18" version="1">MAISEFEKQRQANIARNKELLRKLNLTELANEFGKTENGKNKLKPKSKSKNQSPKVKVEKDPVLPLRRSRRIAGVNIEDSSANDALDKLDEDKLEKERLKELASVRLSDDLKLSEVISDPSVLNRLGKSFSMGDFYEEIKNKPLTSKTEEQAREELDKLELYEDFHPNQIMLTTARMTTIEFHPSAKRKVVLGGDTNGMLGIWSVDDDSEDEPAITRFNPHRKNIARIAVRPGVPEEVISSSYDGSIRTMDLQKNLSKCIVEFDDQWGDASGVSDFKFIDQNAGYLTTLNGEFATFDLREPNVRRENCKVYRLHDKKIGYFSVCPTDEKMIATASLDRTMRIWDLRMIRDQVWSDYEDVKGPQCVAAYRSRLSVSCTDWNKHGDIVCNGYDDSIRIFHMDDGKGISSNISKLAKPEEGDIAENLEPDVTIKHNCQSGRWVSILKSRWQENPKDGVEKFVIANMKRYFDIYSRDGVMLAHFGDERMTSVPAVCSFHPTENWVVGGNSSGKTFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHA1</accession>
    <name>A0A1E3NHA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA mismatch repair protein MSH6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA mismatch repair protein Msh6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_19754</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016617.1">
        <property type="nucleotide sequence ID" value="XM_019162138.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NHA1"/>
    <dbReference type="GeneID" id="30178825"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032137">
        <property type="term" value="F:guanine/thymine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032138">
        <property type="term" value="F:single base insertion or deletion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000019">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000771">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057927">
        <property type="entry name" value="RAD24-like_helical"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25812">
        <property type="entry name" value="RAD24_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="13">
        <location>
            <begin position="943"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="678"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1485" mass="169507" checksum="C5DAE4C9351F5032" modified="2017-01-18" version="1" fragment="single">TPTTENETAMKNIPSSPVAARRSRKVNYSELSDQDEEDEEEDGDIVKPKKKRKVIAWDDADDEDFDLGMAKNKDDEDEYNSGSDESDDDDANNDKSEIKDELTELTDKTVVDDDEDDIVEDEASKQTKEAKPVVTRKINNLSNILSSKPRQMSASMTPQKKFNKENEERYHWLIDVKDADGNLESDPNYDPRTLYIPSSAWLKFTAFEKQYWSIKSKMWDTIVFFKKGKFFELYEKDADVAHSKFDLKLAGTGRANMRLAGIPEMSFDLWTKRFIDAGYKVAKVDQKESLLAKEIREKNGSAKDAKESKVIQRELSYVLTCGTLIDENLLDDEMSKYCLAIKETVDYENNTKVFGVCFVDVSTGNFQILQFVDDFECSKLETLLSQINPMEVLVSKNEIDLLTLRILKFNSNPNASFNFIKPDLEFWGHEKTVDELSKFNSSYDLKTPEILKSYYEKENFLAFSAFGAIFWYLRSLKLDENIVSMGNFGEYDPFSKKLLNSSMRLDGVTLQNLEIFNNSFDQSDRGTLFKILNKGLTPFGKRLFKSWVIHPLLNKTAIDQRLDSVELLLNDGDLKYLIESKLKGIVDVERMLTRIHSKILKAKDFVRVIDSLQVISELVNELNKFGVDSLQGMLKKLIVDFPILELDELLESWNDKFDKDLAINEDIIVPKSGVDEEFDESNAKISQIEEKLSDLLREYKTEYKNSEICYKDSGKEIYLIEVPIKLASKIPRDWQQMAATSKCKRYWSPEVKTLVKQLMELRELHKSICLNLKDKIYSNFDKSYDKCMKIVSTIGKIDCLVSLTRASEALGFPVCRPEIVDNEYSFLDFKQLRHPCFIPGSGILNSKDFIPNDIALGLNNENSMGLLTGANAAGKSTLLRMTCVATIMAQVGCFLPAESARLTPMDSIMTRLGANDNIMQGKSTFYVELLETKKMLDTATPRSLIILDELGRGGSSSDGFAIAESVLHHFATHVQSIGFFATHYANLGQSFVNHPKITPLRMSILVDSESKKITFLYKLESGQSNGSFGMHVATMCGIPKQIVESADVAAKKWEHTSKLHKASLDSASKMPLGLESDFSWWSQNKALTPSFGPFFEKEALRSAKRKPATEGTRLFDETVFNGKKKDLNFEATEKKNILGTKRRKIDEVRKAQNINNKDIWYENNGWKRVRFNKVAKTFLKKALSRALSLEGIKKNKVIELQVERLSSLGDLRNAINTLEFWFKFQYESGVAKDYDEDSIDGKETGLDIFHSIGKIIYGTKHEKEEFEDFRKRHNIKIDVSQVESDVVTVDNVSSEVMSHLTRFNLCCLENYSLTNPPICDDLNRLMDILSISDDMVKRSGGYGNSSVLQNTSFYNCFGLRIHCQSLKEQGEGNLPLNSSSRGKKRVIFSRDSKLRKKLHTIQNEIFDYQKKREKSMVSAKAYAHLNQLETVLIDGFYQSNILSSYKYKYQRYLKGQNILKMERIGGKFTNSITADDEFKVDNEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHD1</accession>
    <name>A0A1E3NHD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein mL59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_35272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016668.1">
        <property type="nucleotide sequence ID" value="XM_019162932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHD1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHD1"/>
    <dbReference type="GeneID" id="30179619"/>
    <dbReference type="OrthoDB" id="18529at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037507">
        <property type="entry name" value="Ribosomal_mL59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040922">
        <property type="entry name" value="Ribosomal_mL59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18126">
        <property type="entry name" value="Mitoc_mL59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL59" evidence="1">
        <location>
            <begin position="21"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="165" mass="19113" checksum="7CA0FB307567A634" modified="2017-01-18" version="1">MSASKLFSVSSAEAFAKLPEKLQNFFTKFPPAPIRKYADQPTACNAEDANPFLANRHPVTGRTHEPLYSLRRQSDLYKLAYKFGIADLMPPLANGKKFFAEKHEASPILKGVLYPKGHKWERTFAERKKKIQDALGEVDNILIKHRGAKYRKRLERREKEEKRWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHH9</accession>
    <name>A0A1E3NHH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_12041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016695.1">
        <property type="nucleotide sequence ID" value="XM_019159875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHH9"/>
    <dbReference type="GeneID" id="30176562"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133370" evidence="2">
        <location>
            <begin position="29"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="126" mass="12802" checksum="61BF847C53B7833B" modified="2017-01-18" version="1">MCPGLSQHASTLSTGHILILHSALLTLAVVQLLPGQAGMGTPSGRSGGEGGGNIQQGPDSWHAQRGLHPALLLSTGENVLALLLFGAGTELSGTELQGSIGSRSMWVPLGVRCSSIGESPLVLNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHM5</accession>
    <name>A0A1E3NHM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_22857</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016737.1">
        <property type="nucleotide sequence ID" value="XM_019162281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHM5"/>
    <dbReference type="STRING" id="763406.A0A1E3NHM5"/>
    <dbReference type="GeneID" id="30178968"/>
    <dbReference type="OrthoDB" id="284292at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="13440" checksum="73D3D1FE7D7A8E52" modified="2017-01-18" version="1" fragment="single">VVIRVQPLQREGESIEQLRKRLVYQSRKRGILETDLLLSRFAKKHLDTLTKEQLEEYDKLLDELDWDIYYWATKNFEITPLPDRWENSEVLRILQEDAKNKEKEILRMPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHR4</accession>
    <name>A0A1E3NHR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016749.1">
        <property type="nucleotide sequence ID" value="XM_019164399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHR4"/>
    <dbReference type="GeneID" id="30181086"/>
    <dbReference type="OrthoDB" id="4036575at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="561"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="594" mass="64151" checksum="E84EA3F32643D33A" modified="2017-01-18" version="1">MTFNFSSDLVCYSLSMNNNVPTSTLDMNHYYPYRQQNGTADQPQPLGPPAAPSQQQRSGAQANPPQAPASVAKKPSKLDVLIQTATSVDLDSFLPSTISSNEKKRKLSSSFNLEYLCNQAEKELNSSCSSASSSSSSSSSSSSSSLASVSELSKLQSQSGQQLKKANKKSNRKNSISTKGPKANLHQPHQKHVPPRSADLSQQQQPSAASTYGAAPNPLAIKLASPSTHTKRQRSGPSCDCCRSRKIKCDSEIFILSALDTINLAENVKPKVINSHIAHCDFISINPKSGYQYYKIIKDEEHALNNKLTSFNYLQFKPCSACMSKKLKCCFSKGFTRNDIIKFNKTEKMNGTPNTFPFTSATPTADSSVLPSPSTSIPASPPSSAASSSASIPTSTFPSSQTCSFSASGQDNTSLPSNSQISVKLEVPGDSSFISASKSISSALPTPSPTPAVLNNNILSSSSNAAAILQTTSSSPSAPHLAVPNGMAVDRALIYTPHLRDSSSLDMVKHESQPQTQPQFQPQFGKFNESLKPPKSTELKDKEKEKEKELEKERKSSKKTSCKTCRFKKIKCVKIDGSDRCVYCNKKSICCLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHX9</accession>
    <name>A0A1E3NHX9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ethanolaminephosphotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_27554</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016852.1">
        <property type="nucleotide sequence ID" value="XM_019162501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHX9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHX9"/>
    <dbReference type="GeneID" id="30179188"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101026">
        <property type="term" value="P:mitotic nuclear membrane biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="44243" checksum="7C7AD775CFAE688C" modified="2017-01-18" version="1" fragment="single">MSTFIPKNAIKHLNEYRYQSEDRSITTKYILKPFWLKFVNVFPLSMAPNMVTLLGLLFIVVSDVLVFYFDPYYDTVSPQWLYFYHAFAVFMYQTFDACDGIHARRTGQSSPLGELFDHCCDSMNTTLMVIQFSSAANLGTDTPLPFLVQFSTLANFYLSTWEEFYTHKLFLSEISGPVEGLLLISGVFVMTGVWGVDFVWKRELFTLYLSDSIAPIVITPTYVSGAIGGFIMAFNIYSARRNVINCLKETKREKEVSDALKGIYPFFIYYLTVAILFVLHPAMVTKYTTTMLLTIGSCMSFTVGKIITGHLTKQEFPYSNFPMYAPVVQAVLMQLMVSVFGADYEEALAMVVYGGLGASMAIYGMFVYEIIYDITDYLDIWALTIKHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHZ4</accession>
    <name>A0A1E3NHZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_17028</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016862.1">
        <property type="nucleotide sequence ID" value="XM_019161434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHZ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NHZ4"/>
    <dbReference type="GeneID" id="30178121"/>
    <dbReference type="OrthoDB" id="2534523at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="10">
        <location>
            <begin position="904"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="70"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1034" mass="117311" checksum="B51D2808B0D816F8" modified="2017-01-18" version="1">MFSRGTRRLFACVNRFEWRKVSGKRVLSISLPFRRENHEKSSGDNQLLFTAEDLSDIKVVVPKLKSNSFNEESKTSGVADFDEGRLDSRTLHKTQAKPTAKTKSKRKSRAKKVMGSADSTEPVTEEAIREDKLSFKENQIINEKMEMSISAANGASAATLLDRGAITDNNTLTDFYQSIVQMYNTYSARTDGNYIVLIHVGSFYELYFDQADKYSGLLGLTLTKKNLKLGPISFSGFPDRMLDKYMDIIHRAGYKAVICNQVLDPVTNIINRPVDRIMTPGIIVDESCRDFHRNNYLMALSFPDDLKPDIEGKRVGVAWCDVNLGLFYILEIDFSQLLSTVTRINPAEILISDKADLERVLNGSILPELVDLKSYCITSYHEKSKKKSLDDFLWRFSDNKRLVSSTLDSMSRKEKNATSLLLHYLEVCLPNFKTSFHLPTRSLPNTLMQIDSRAAQDLELLETLQSRKRIGALSHLIDKTVTSPGARLLNTWLLAPSTDLDEIKRRHDLLGVFLKDPYFLDTLVQQLKKTADVNRIIRRIDNSRADSYEYLELAMTIRVIDEIYNLIERSQNNHALKLIEPIFYEFIHSTKVHKLAKQIEETIDPKVSYLKSTSNKLDGELVRDFWEIKETASSHLKKLRKDYDTEVAKSTALKQELQVQFQEEGYGGSLKLLKDMKTYDYVIELKSTSKVIPTMVRNLGLKVKEKSKSVTKLIDSRWTNIGECLVSLEYDILLEEKVIMNDLNSKISKLYKELRKLSPIIEVLDVIQSFTQLTLQYNLSKPHVDESTIFDVKNGRHIVVEEGLKNRFDVVNFTGNDCRIQSSEAWLITGPNMGGKSTFLRQNALIAIMSQIGCYVPAEDAHIGVIDKIFTRVGSSDNIFKHQSTFMVEMNETATILREATEKSLVVIDELGRGTSANEGVAIAHATLLHLLKANKAKTLFATHYGSELLHLLAHDGNVANQVSFYKTSLCDSYDDGDKAKSEDFKSIDQRLFFDHKLTKGVSLHSHALKIAELAGFPLEVLHMAKSSFKKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHZ9</accession>
    <name>A0A1E3NHZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plasma membrane proteolipid 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_34378</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016872.1">
        <property type="nucleotide sequence ID" value="XM_019162883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHZ9"/>
    <dbReference type="GeneID" id="30179570"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF112">
        <property type="entry name" value="PROTEIN SNA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9148" checksum="E63E169684F43E14" modified="2017-01-18" version="1">MHARDWLLAFIGVFLPFLPVALKRGVCSADFWINIALCLLGFLPALIHCWYIISCYPYESSSSYRGVTLPGREPHGYGAVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI67</accession>
    <name>A0A1E3NI67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17083</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016927.1">
        <property type="nucleotide sequence ID" value="XM_019161457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI67"/>
    <dbReference type="STRING" id="763406.A0A1E3NI67"/>
    <dbReference type="GeneID" id="30178144"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="313" mass="35045" checksum="3188773964F97C0D" modified="2017-01-18" version="1">MASASSVTVKLNTGAEIPLLGFGTVSEDNVENFKEALTAALVDAKYKHIDTAWWYNTEPYIGEILESLDKSGKIKKEDIFITTKVWPCFWNDPENSIEISLKNLKLDKVDLLLQHWPVCFQKVEDSNGRVGLPKDAAGNILYDKNGDYLVTYKKLLKLKDEGKAKAVGVSNFTIEMLERVIKETNVVPACNQVEMHPHLPQLDLYNYCTEKGIVLEAYSPFGSSGAPNLKIPLVKELASKYDVTPADIIVNYSIAKKIVILPRSSNAERVKKGYKLINLEKSEVDALDQFGVTHPQRFVNDPWGKGLGFEHWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIA5</accession>
    <name>A0A1E3NIA5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_35104</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016983.1">
        <property type="nucleotide sequence ID" value="XM_019162923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIA5"/>
    <dbReference type="STRING" id="763406.A0A1E3NIA5"/>
    <dbReference type="GeneID" id="30179610"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000005">
        <property type="entry name" value="G2/mitotic-specific cyclin B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="122"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="215"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="219"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="339" mass="39771" checksum="C80679A10B75B499" modified="2017-01-18" version="1">METSNLLEGTYESHNDQENFGLESNVEDEDEDVEDDLIYNEPLSPVLNDSIKKEIDRVYSKFSRDTLDPNDEDTYDVTMVAEYGNRIFNYLHSLEIRYKPNPRYIEEVQDDLLWEHRATLMDWLVQLHSRFNLLPETLYLAVNIIDRFLSKKTISLSRFQLCGAVALFIAAKYEEINVPTVSQMVYMVGNQYPRESFLRAERFMVEVLGFEFGWPGPMSFLRRGSKADDYDNEIRTLAKYFLEITIMDARFVSSPPSWLAAGAQFLSRRMLGRGDWTEAHIYYTGYTAEQLVPLAEILEECCISTETHHRTIFQKYSERRFKRSACYVQEYLHELYGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIB0</accession>
    <name>A0A1E3NIB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_59477</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016982.1">
        <property type="nucleotide sequence ID" value="XM_019163370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIB0"/>
    <dbReference type="STRING" id="763406.A0A1E3NIB0"/>
    <dbReference type="GeneID" id="30180057"/>
    <dbReference type="OrthoDB" id="407959at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="14553" checksum="B35D8FCC40AD1472" modified="2017-01-18" version="1">MSGSGAQGTKFRMSLALPTGAVMNCADNSGARNLYVMAVKGNGARLNRLPAASAGDMVMATVKKGKPELRKKVMPAIVIRQSKPWRRRDGTYLYFEDNAGVIVNPKGEMKGSAITGPVAKECADLWPRIASNSGVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIM7</accession>
    <name>A0A1E3NIM7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_27772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016551.1">
        <property type="nucleotide sequence ID" value="XM_019162513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIM7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIM7"/>
    <dbReference type="GeneID" id="30179200"/>
    <dbReference type="OrthoDB" id="10267139at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="entry name" value="CofD-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240:SF0">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="CofD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="377" mass="40837" checksum="DF835F7878BC3787" modified="2017-01-18" version="1" fragment="single">DITVISGGTATNHILDAFHPDDGYTVTYVLPVSDNGGSSSEILRVLGGCAIGDIRSRLVRLIPSDHHACGIRGVRDLLSYRLPLGTHQAKYEWSQLVDGTHPIWGPVDSALKVLLLSFLIHVDMEIHKRARLAFRFELASVGNLFLTGARLFFGDLDSAIELICRVCRLHEHVSVCGCLNTNFTYHIAAILCSGEVIRGQSQISHPSPAPAVGPAADPGQRPPRAQHNRSVEDLVGSSLAHPAPASDDPIHPSLGRSQLHFEKDDRNVPPLPSPIARLIYISPYGEEIHPRASPRTLAALARSDAIVYSIGSLWTSVVPVLLLRGVGEQVVRRRPRGQPPVRKVLLLNSDSDRETRGLDGAGFVRVVVAAVEYSLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIN8</accession>
    <name>A0A1E3NIN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Skg3/CAF120-like PH-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_148373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017094.1">
        <property type="nucleotide sequence ID" value="XM_019160559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIN8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIN8"/>
    <dbReference type="GeneID" id="30177246"/>
    <dbReference type="OrthoDB" id="5563754at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058155">
        <property type="entry name" value="Skg3/CAF120-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25381">
        <property type="entry name" value="PH_26"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Skg3/CAF120-like PH-like" evidence="2">
        <location>
            <begin position="312"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Skg3/CAF120-like PH-like" evidence="2">
        <location>
            <begin position="507"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="443"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="679" mass="75446" checksum="CB8871AA47D2E7EC" modified="2017-01-18" version="1">MGFLKLFKKKEKKDSVEPAQQKTGSKIEKGDPSSRAVQKASGSNSQKTGAEALRNGGGSEVAGSRTIGPDLSVGGFDTFASGANTSSSNSGSISKTDFVPPASSATTQSSRKSSVSASLKKDNTDKRKSSFGILRKPSFNHSAPITENNTPINAHKVSDLPDELLPVVTLINHQQSRSYFKGNSFFYDSTQAQPILNPVELVSNATTPVSLLTASFNWAPAYVELNGNDITVELENSPTTIINICDCELTYNGDDIILTVLITNHSLMYFQFNTLDELNSFYSAVLLCKFEYQQLQEAYTGALLSSQAIHFSDIRTLLSPHNKNVKEEWCVVRFPFLNDKWIRCYLVVQPHNKIEIYTHSSKARKYLLSTITNGLSCYTIYPNDPSQVQNNSLLRLYANCYINSDLLEVILNDDTISSTDENISLMNKKSVKTRSSRSRSNSRSRNNSFNRRLSQVSLRSSRSHDSNLSSSNESSNLSSSHQHHQRISSVDTTISDSSFTNAKTPKKLSKKSIAKTHLIYIIPESHASVKPCEISLRLLIPILNSYKLYGRPAKFISSRTDKNSLLFGLPQLPNTYYLNEKSSLDLINLNIGNSLREKWSAHDWYMIYKELMSALLSKGWKGGSYQGDLVNMNFSLDMDRSPSQFDFEDDTYDPMEDFVAAASSSANRSVSVPVSLVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIY2</accession>
    <name>A0A1E3NIY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016426.1">
        <property type="nucleotide sequence ID" value="XM_019164669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NIY2"/>
    <dbReference type="GeneID" id="30181356"/>
    <dbReference type="OrthoDB" id="10018316at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052113">
        <property type="entry name" value="FYVE-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490:SF8">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="577"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="139"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="385"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="388"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="728" mass="79216" checksum="D44323803F994596" modified="2017-01-18" version="1">MASNKTNCSLNLQPMNSGNYTAAIAGVTSSDTDEHTVFGRNADGGGADGVGADGGDGGAEKEAAGKQYLRGGQAGNRVLAEEDMIPPYDSSMCTNNSLINDSSVLLASSSLDLKPQKKKGAQETPAEHHAADADVVVAPPLLGSTDNGDHQSSDRTESLKSTVSAESRGTEETERTSGTSATNNTHATNDLNEETRQLSDSSSGASERRGQASSQYRKDTLTDNTLVEKQFDTMNLRDAGALTQKSEQVEDGSSESQSQPEIPAPSQDQPQRPGPLQPIQEGVQSTEQGLQGKQQQPRTQQGVYSDDCKQQRQAYEQSLEQKKMEQFRRLNMQEKSESRGFAKSVSPMSPKTTAPFAQKLPAAAKSGAPLPVIPSKFSKPVVESSNASLSSTKLTGSKTSSTPITAASHATSKPGTNTGCNSHLPDTKLINSQTNNPHAGGNSISYSNLSMSSNLGNDNSNGTFNVSPQAAFSPPKRTQSPFRDLSNPYLKQINNQQRPLYTPAVLRVMKNFGTSPSSEVGFDVHQLPSERPPLSQSSSAISVRSTASSIVDYWNYMTGRTPSKSYEGPTREHWKPDSSRFNCAQCGKLFNYMTESRRKHHCRSCGDVFCGDCLKNYIYLDRSAHFTLFGSNWDEHEAEQKPNGLVNLVDENVGKPNNTPKVAVEDDKKYLCKVCLNCFQQYEEYVLDHTTRDHNLGADGKDMRKNNEHRETTMDTKNIPVDWNWSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJA6</accession>
    <name>A0A1E3NJA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017304.1">
        <property type="nucleotide sequence ID" value="XM_019161090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJA6"/>
    <dbReference type="STRING" id="763406.A0A1E3NJA6"/>
    <dbReference type="GeneID" id="30177777"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF3">
        <property type="entry name" value="HEXOSE TRANSPORTER PROTEIN (AFU_ORTHOLOGUE AFUA_8G04480)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="453" mass="50721" checksum="242168A90370E918" modified="2017-01-18" version="1">MNHPTGTWLGFISASPFVGTCGSIFFIPYFCDRFGRKAVIYATVLFIWIGSIVGASAKNPASYIVSRIFMGLSFSCNYSASIWIGEISYPTTRGIFSACYHAIYYFGSVLCAWVIFGTRVCSGDAAWRIPVGLQAAIPTILLPALIYAPESPRWLASVGRFEEAEAVLLKFHANYEEVYIPLVQLEMEEILAAMEIQKKNNISSWKSLVATPGNRKRLFISCFLGVAFAWNGQGIVTWYLSTVFKDVGILEVWKQTLLNACIQLFNLLVAITGAFLVDIVGRRTLFLISIIGIFCSYIGLTVITAVYKELHKEIGQAIIVFVFFVYGFQAIAFAPMITSYPNELWPQTTRSKGLFSAMFFLNGATFFNIFVNPIAIEAIGWKYYCVYLGVQVIIFLVIFYTFPETKGHSLEEIALIFDKDDWTQSGLHKEQTSLEGKKLSSIYVTEVSSKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJD1</accession>
    <name>A0A1E3NJD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_72335</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017365.1">
        <property type="nucleotide sequence ID" value="XM_019164162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJD1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJD1"/>
    <dbReference type="GeneID" id="30180849"/>
    <dbReference type="OrthoDB" id="10249365at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02773">
        <property type="entry name" value="MopB_Res-Cmplx1_Nad11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.210:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="12">
        <location>
            <begin position="30"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="14">
        <location>
            <begin position="108"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="247"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="727" mass="79355" checksum="A0F6B05D8BA1F03A" modified="2017-01-18" version="1">MLRSFNKLAISKSIAKQATRSFAKSSIAKQDVELKIDGIPVSIEKGSSIIQAAEKAGVYIPRYCYHDRLNVAGNCRMCLVEIEKSPKMAAACSMPAGNGMSVLTQSDKVKKVREGITEFLLSNHPLDCPICDQGGECDLQEQTLKYGADRGRFHEVSGKRAVENKAVGPLVKTSMNRCIHCTRCVRFLNDVAGAPEFGTAGRGNDLQIGTYVERNINSELSGNIIDLCPVGALTSKPYAFKARPWELKRTESIDVMDALGSAIRVDARGMEVMRILPRLNEEVNQEWISDRSRYACDALKVQRLTKPLIKQGNKFIDATWDGALSLVADSIKKINPAKNEVQAIAGPLVDVEGMVALKDLVNRLDSENLTIDAPVTDLPSFDIRSNYLFNATIEGIDEADQILIVGSNPRHEAAVLNARIRERWLESDDVEIHHVGEKFESTFNLNQLGETTTDLQTALKGKLGEKLASAKHPLIIVGSGVWENADSAAVESVLKDFVRGKENLINPEWNGLSFLHRQASRAGALDIGFYNADPAVKAVVPKVIVLFGSDEIDPKSIPNDAFVVYVGHHGDLGASLADVILPGTAYTEKSATYVNTEGRSQMTTAATGPVGSAREDWTIFKALSEYLGVGLPYEDDRALRSRIETIAPHLLRIESVEPVSPAVAKLGLEFIPSAKPTGSKLENPIKNFYFTDVISRNSKTMAQCVGSFGPTVDKVNDENAHQYLGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM8</accession>
    <name>A0A1E3NJM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nicotinamide-nucleotide adenylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72368</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017399.1">
        <property type="nucleotide sequence ID" value="XM_019164178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM8"/>
    <dbReference type="GeneID" id="30180865"/>
    <dbReference type="OrthoDB" id="5591297at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285:SF0">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30889" checksum="9CAB712AED8C73D7" modified="2017-01-18" version="1">MEALKVVKTFVRSGDSFRLVKCYGDRDVVGPTTRRVLVLDSSFNPPHRGHLAMIKQGVLSLSTHDGITTINTNAVLLLLSVKNADKDSVSVDEYAQRLHMVGLMAEHVCAGLGLSCGVALTDASLFVDKSRLVADWTGRSSGVDNFFLLGFDTLIRFFDPRYYREPLVEAVDPFFRHSKLCVLLRDDKSSRMDLADQKKYMQDLQNGEIDDKDNNRTALPASWRGKVFFCEAKHEWAISSSAIRTAVREQDPVRWQGKVIPTVRAYINGASLYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJS3</accession>
    <name>A0A1E3NJS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA wybutosine-synthesizing protein 2</fullName>
            <shortName evidence="6">tRNA-yW-synthesizing protein 2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_59443</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016931.1">
        <property type="nucleotide sequence ID" value="XM_019163367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJS3"/>
    <dbReference type="GeneID" id="30180054"/>
    <dbReference type="OrthoDB" id="2387925at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026274">
        <property type="entry name" value="tRNA_wybutosine_synth_prot_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF25">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038972">
        <property type="entry name" value="Trm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="7">
        <location>
            <begin position="137"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="432" mass="49087" checksum="0532C2C7BDB66D80" modified="2017-01-18" version="1">MCVRLPVACVLAVENKKEIKRIKSLLESRSCLDKSTKIFSDPNGTFLIPILIPQIANDDPSAFIAKLLPGEKVSVVRSTAETGSSDIGSKLAHLLRSLDPSLDDSQVDGLLKKFPKRYSVYPPLLLLPQKSLDSKEWSCFLGSLDQNYRSEIFSFILRHFSTPTNTLTHMAINKPIPETNNEIRSPSQLTTLYGDFTDFWCHTTQNGIYQTWMPAYTMFSRGNIKEKARILNSYQNITSDSDIVDMYAGIGYFTLSYLRRGARRVFCWEINPYSVEGLTKGVRENGFGEAYVIKREQHISLAKVMKARCVIFLESNVHCLERLEEIKSQVHDGDNLDLNISHINLGLLPFSTDSWPYACELIDLYGDRHSNAWIHVHENVAVTQLDTKMDSVKSELQKLSNTRKVIPVWLEKVKTFAPDVYHVVADFELEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK45</accession>
    <name>A0A1E3NK45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_130945</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017603.1">
        <property type="nucleotide sequence ID" value="XM_019160011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK45"/>
    <dbReference type="STRING" id="763406.A0A1E3NK45"/>
    <dbReference type="GeneID" id="30176698"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="3">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="429" mass="47546" checksum="82AE68299021F4CD" modified="2017-01-18" version="1">MQSTVLPPLGSPLPFPNSSDVAILKYVGSVSGHDGVFCGLELCGALAARGKNNGSVNGMAYFQVDTPNSGLFVPLRKVIGWLGTSNVNGNGFSSSNIFSPPNAQQQQQQQYGSPAATLSSLGNGNPAVGENSNELRRHISVLEKRLVQRENDLRELDVQLDELDATLRTNDARLARKEERFNRYKNDKEDEIKLLVGTIEALEKKVGDAEETYAVKLRQLEQQKLGSPGSNTTALEEKIKTLEQELSVQKEMHASFKISKTMEINELRKAEMEAYKLNEELERLREESVDVVEAENLKRQNEQLVQELESKRIDIELRDTKIKSLEELLKSSRGENDVLRNRMQSYERGVGSSSGSGVGVVSQQGPNAPSSATTMSNQNLLDENGGLKVFVPEEKIDPSAGREDFCSFCDREGHSTDDCPYEKDNIEMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK62</accession>
    <name>A0A1E3NK62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_58814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017640.1">
        <property type="nucleotide sequence ID" value="XM_019163321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK62"/>
    <dbReference type="STRING" id="763406.A0A1E3NK62"/>
    <dbReference type="GeneID" id="30180008"/>
    <dbReference type="OrthoDB" id="16816at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050602">
        <property type="entry name" value="Malonyl-ACP_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090:SF1">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="41191" checksum="2FF0AC8E0EDD7EA1" modified="2017-01-18" version="1">MLGRSLYNRLTLRYYSQKTGGANNVKVFDRHVKWLQKERAAHNPNSKFAEYLRDEINRRTIDRLAFLKTDFTNVLDFGAHAGNFEKLLCDPSRDTQNSQWKADRELIKSKIGKIVMVESSKSLLYKDLTSAFNNELNIDRIVADEENFDVPELSEPNQYDLIISNLSMHWINDLPEVYKKLHNCLKPDACFIGSMFGGDTLFELRTSLQLAEIERYGGLSPRISPFVESSDVGNLMQKAGFQMLTVDAEELIVDYPNVMALMEDLQLMGENNSVLMTPPPLTKDLLMAVEPIYREMHGDKKTGHLPATFRFVYMIGWKQGKYLAKPAQRGSANVSLKDALDGKTITTTTTLTEDEEPTTTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKF2</accession>
    <name>A0A1E3NKF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_132852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017748.1">
        <property type="nucleotide sequence ID" value="XM_019160038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKF2"/>
    <dbReference type="STRING" id="763406.A0A1E3NKF2"/>
    <dbReference type="GeneID" id="30176725"/>
    <dbReference type="OrthoDB" id="2100128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="35978" checksum="C1122FA52AF007AB" modified="2017-01-18" version="1">MSEGRHSANNWRGGGRGRGRGRGRGRGRSQEADVLNSARPRVASGDDEALRRRQERFARDLEREAGSGNGRGDSYGLISRGEDTRLKDDATARQRLLDDTLRRVHEYERKAAAGGTAGADSERDSILLAFRKLRESVVAVDSSRRAAGAALSEPEKSFFREVYLSSIRFGVRCGSHESYVPALQWVLPVATAGRWAFTASEHEWLVLRLAEQLAVVEQRYEESLLLLNTLLHFKPVDLNARHASGPLEQLYLAVVKLINNNFHVAGLSVRGAARVAQLQAQQRILSYRGSERYEEQDVLKKGTERETGSEKKKEGGDGAEPGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKG1</accession>
    <name>A0A1E3NKG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA polymerase V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_151420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYBBP1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017161.1">
        <property type="nucleotide sequence ID" value="XM_019160684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKG1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKG1"/>
    <dbReference type="GeneID" id="30177371"/>
    <dbReference type="OrthoDB" id="342531at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007015">
        <property type="entry name" value="DNA_pol_V/MYBBP1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213:SF2">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04931">
        <property type="entry name" value="DNA_pol_phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="771"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="771"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="816"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="851"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="862"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1088" mass="122301" checksum="5559737FC211ED68" modified="2017-01-18" version="1">MGVSKDHYYRLASNLAKERIQAATALIKELSAADSEQEYRYALKRLISGLASGHDSARIGFSMCLTELLSLLFDADNGTKAKIAYTSEQYLADLESHLTKQVKDKQKGKNLRAYLFGKIFGIQSLINSAVLHKELAAGNSHLITHIVDELFAIALSKSWIREIALVTMVKLITQLRLATDETIMPHILKSLSDNDLLVSMDGLLIYLTIPQTHRDSLSALAELKPSQSWKNNDPITRGNLPLLNAAFHDRNVSPDSEEDESSKDENDEGRAKKKGGKGGPASGQNQKGSWNPQLHNVWVPLLSELIENELQLQDPLSGEPSPKKRKSNKHKSKSSESNKISLSTFWPTFDDTFFAQSASPERKHTGLQILDLCFNLPTFRPSFFLIVFGENITRCIINHISKKDRVLHNLCAKILANAVKCCKKHPEVDSRLYLLRALERECVLFDRITKTKTVRDCISGISSNDNDLEKDVTNWIDIANWLITEAETELYESSNKAKNEKFRNEHDIKTFLLDALLTLVRGNKKLFRSVVDGESKELTLEVVSSCKMVLEYLSQMTYVNSSAKEVDETIIKTAKDRLQSILAELMECFKGQVDWSGLIVHVLRDAERDNILRAQIGESDELIQTKKNAVEIWENLGKLVKKQVTKKSSSSAERQLRVNKCLVMLFSTALLELYGGDSESFGILSDLINVYEDLNNQQESEDNNIIDTLVDLMLSYLTQQSGLKKRVGTYIWESVLHDVGEQQLERLFDVLMTRENKLGMEQLFNQAVEDFEEEGDDDDEEVEEEEEEEEEEDEHDDDDDNDDEESDENSEIDEDDKSKIEEVERSTTSALAKALNVKEDADSDQAGSGKSTEDAEGKDNENGDEDDDGDDNDDDSESDESMSDEQMMAIDSQLSAIFQQRKSVLDSLRSSSKNGNERKLEAQEARQLMALCKLRVLDLLEIYVGAYPTKKEVVSIATTGLDVMKLTVDLAVGEKTHKLIKKICKNVFTIGNSKEKEAVLETLRKVLERASEAKFNALSQGCSQVAVYLVRSVCASDEDSYESNLATITGIYQKHMVKWAVDSSDRSTASIFTDLVNWLSSKKSAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKH4</accession>
    <name>A0A1E3NKH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_33424</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017768.1">
        <property type="nucleotide sequence ID" value="XM_019162823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKH4"/>
    <dbReference type="GeneID" id="30179510"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="27000" checksum="3E89DAFD2F2287EB" modified="2017-01-18" version="1">MQLRCGWDHCDFTTIDIDKFLEHVPEHTELLKRTNLDDSEEDSDVHVCHWVNPETKSECGEHFDLTEDLTNHIITSHIKSGKSSYTCHWKDCCRGEKPFSQRQKIIRHLNTHTKHKPFECEICHKKFSLDLMLKQHMRIHTGEKPYKCDLCGKTFKTSSSLTIHLRIHSGAKPMVCKICGKRFNESSNLNKHMKIHFRKYRCELCLKSFDSETKFKRHQLVCRKKSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKN7</accession>
    <name>A0A1E3NKN7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TrmE-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_33426</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017825.1">
        <property type="nucleotide sequence ID" value="XM_019162824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKN7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKN7"/>
    <dbReference type="GeneID" id="30179511"/>
    <dbReference type="OrthoDB" id="188276at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14858">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="entry name" value="tRNA modification GTPase MnmE domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003661">
        <property type="entry name" value="PRK05291.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF2">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TrmE-type G" evidence="6">
        <location>
            <begin position="257"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="506" mass="56331" checksum="10E13548C9B738E8" modified="2017-01-18" version="1">MPQFTYLRKFGTCLSCKQSINYASPTVYALSTAPARAAIGVIRITGPASKKIFTKLTHSSSTPQHRVASVRKIYDTLTTSDVYGERKSKKLLLDECLVLFFESPKSYTGEDLLELHLHGGVAVINRVMSTIGKLHSPISPIRLAEPGEFSKRAFQNQRFDLTEVEGINTLIHAETEMQRVSALSSMKGETNILFNQWRERILRNIALLTTVIDFGEDHDIDEVNDLFDHVNIDIVDLQKEVEEYLLKVRRSQILLDGIKITLTGPPNAGKSSLLNILAQDDKAIVSSIAGTTRDAMDVPLDINGYKIIIGDTAGIRKSTDEIEKEGIKRAKKKSMEADINILILPADELDTETEFLAHALSLVRNSDKENMLIINKSDLISKEMREEILVLFSERLGIPQESILFISCTENEGIEKLIGNLTARFEKVTNSDKEDPISISNRAKEILANDVLYGFSEFYKFKEIDDIVMASEGLRSSVEGIGKITGQAIGVEEILGVVFSKFCIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKN9</accession>
    <name>A0A1E3NKN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRO domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73382</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017006.1">
        <property type="nucleotide sequence ID" value="XM_019164466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKN9"/>
    <dbReference type="GeneID" id="30181153"/>
    <dbReference type="OrthoDB" id="2141925at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="7">
        <location>
            <begin position="3"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="765"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="765"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="797"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="818"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="850" mass="96198" checksum="6201E2A742851454" modified="2017-01-18" version="1">MTTFIHLPEKSTSKADWEASLNKFLNLTFGKSFTNEISSSIQRLNKLRVDIQHSHTSDGESLIPIYLNYITQLNSLELRLPMSAVITGGELEFSWTDAFDSNADPTVQHSLSFEKASILFSLASVYSYLAATASSSLDWKNAILNFTNAAGILDFISINFLHAPTNDLKVETSKGLAKLMLAQAQECFLWNYMQSTTGNIKHSLVSRLAEGTSLTYLSAKELISSCKSKLDCDEEIHIKGLYFHTFALFHYAQSYMETMKIGYAIAANQMAKDSYAESKRLIRSYRGKLEPGIVKMNETIVQEIEDKLTEWEKDNDLIYHQAKPEKNNVPAIKSMDGAKSVPFESQLKKSNFKDLFEKIVPMEAHKGMSIYSEKQAQVTREWNEKIEVANEEIKSFFEFSRLPTSVVEVQSLLRTSNSSIEEEEREKEDNYPRVLAMAHELESSPDVKFEQVMTNVQNKRAGILKQLEEAESWLLKDERNTVIKGLPPNPELSKLKDEITMTRNTLAEAEISDTKLNDLWSKYKSEIRVLQKGTSGVQKWLSESENKNAENLAKQVSLLDLDDTGDKNDEYDLTIAKRLIENIYTMKRSLELLMDERNSTLNDLKTSMHSEDISSILIQYSGASESELNKVFDEQLSKYDSFTSRLDALTSAQDDRIVELKDSLGRLLDLKIVRKKVEEKKKERGTIKAKLASLLSSYDTWKLCSKGADEAYGFYCRLSDRTASSVGRIYDIISQREAMNIEPRSSISSTLSGGFGGHTINNYYQQQQQQQQQQQQQHYQSQPPPSYSNSLYPNPMGINNRTDSVSSAVSNAPPLPSKPSTTGSDNSQPYSTPSVYNPNMYAQFGQNWKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKS4</accession>
    <name>A0A1E3NKS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_11696</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017833.1">
        <property type="nucleotide sequence ID" value="XM_019159826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKS4"/>
    <dbReference type="GeneID" id="30176513"/>
    <dbReference type="OrthoDB" id="4062597at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="265" mass="29527" checksum="6AE7FE150C94DA77" modified="2017-01-18" version="1">MTQDKDYGLLYQAYAFSSPELKKHEDLLVHTINRGYNAPRFQFNVITSPRIKHSVVEDLCVVPEESSYALFCFADPRAGASSTDRTSTVKIDVDELVDDAAAAREKLLQLYPKGTIDTQNPRILYDDSVSPKKGTTEKLCLPAPDNQFTSRFLGMACLKPRGGSGTSYELTGFISFYPGVGTHLIRTVEQFARSQLAATDIWIAAIEEHLLGQMYSKFGYEFVERVLIPVSADSGGKVADTQDHLENDIMASRDFHLEVLCKKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW7</accession>
    <name>A0A1E3NKW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin interacting protein 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_58997</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017882.1">
        <property type="nucleotide sequence ID" value="XM_019163336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKW7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKW7"/>
    <dbReference type="GeneID" id="30180023"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005519">
        <property type="term" value="F:cytoskeletal regulatory protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005613">
        <property type="entry name" value="AIP3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056279">
        <property type="entry name" value="Aip3p_Bud6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF10">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN CG32809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23153">
        <property type="entry name" value="Aip3p_Bud6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00806">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3 C-terminal" evidence="4">
        <location>
            <begin position="309"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="241"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="704"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="515"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="711"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="731" mass="81802" checksum="6D81AF76972EF852" modified="2017-01-18" version="1">MSETDLGSFGRKSPTRRKGHVPSIATATSHLLTATKALLQSLTSWSQNTTSVSDVSSRFVKFGDAYKSLKRSYSTSGVDVTQLPDIPGKVRLILEASLVMEPSQESLDKFLPQIGDSVAELMKMLKEKQNELNTVDKNRKNGSISASSSILSFKQSNSQTTPTTSSSGHSPLDFNRTPSKDPITRLQNNNDLMRRASKRFSAYQTSSIISMQSPMSDNIFNAIDLPKTPLQMDIAPNITAEDLEKAKNSTKLDDVDENETKRQPDLSKVSGKASTNNLTALKEETLSDTAKSLTGGQNHAERNSQSSIFLKLGDQVKKVSIDLPTTFPNLKVLFSQRFNYSSPATSSYPKIYIQDGPNLVAYELENISEIKYGSIISLQQPDFKSLIISHVDSQIGTVKNDMMNMEDRILKKLEKLQALQSSAPATPSLRQSTDIDSGKNMKKLGEKENHQKLFDDLQDELTKVKQHQIKSVRRINGMINSTVETIDRIQADGLASQISENTYVKDCKAKVSEGCESLVEKLDDLQDVIEFIKRDITKHNIKPSDKQLEHLSKEMKNTKAELSSLTDYTQIERKNLLSMWSKQMATISADQRFFKAQEEIMGLLAQDYQSAQETFDLIVSCATQLEKGSLTYKSKLPVPDPSVSPWDASKLVMAEVDAINPNHEERVEAIMKAERVREMEKELRLKDEFQEELGDFVSNEKLKRNGGGISEIEKQRQEKDQEHMKSTFGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL06</accession>
    <name>A0A1E3NL06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16634</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017922.1">
        <property type="nucleotide sequence ID" value="XM_019161287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL06"/>
    <dbReference type="STRING" id="763406.A0A1E3NL06"/>
    <dbReference type="GeneID" id="30177974"/>
    <dbReference type="OrthoDB" id="762982at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF88">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="113"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="215"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="701"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="981"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="723"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="986"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1104" mass="126294" checksum="289ED7094A07C064" modified="2017-01-18" version="1">MSRAGEDLGKSRLKHHKVLPNVHPTNSLILPDGKIFKVQKKRQRKIHSCIPCHQRKIRCSRETPTCLNCLKLAEKNPSEKYEILDACKYFDNDKKKNLKKKGVSFGTSAAAAAATASSSRRSMRTAVSSSGAGHVSNDTEELEYEKLSGEEEQEEGAEELDTAYQKDSGDCQQLAGINNNSSRSRSSSSNSSTTNSQTDAFSSILVTQPFYENDYAGHFGRPTGVGAREGGEEPKTYSNTNPTAGARTDTRDTADLSAEGKTRSKSKSSKIDNTNNDYNTGNTNYTISLGQRNGAGEKYNLVEDNVNEKLGHTNEYTNDFPIDQNEFVYVPRDESGEHMNDAERNGFSYQNPNNLYPDDHIYSHYSKQFASQFITMLNNLPTRERSDELLKNFQTNVHSLLPILDMNGFLEKYDEFWYCGLFLTDNIEKLYQYTIYFKDKYPSVIPGKINDFLYWYHKTNGSLNPSNFSEFLILLYAVYYTSISSSVYEFLSKKYDNFNNILSYKNEVNKYYNMFKNMNHKGLNNPRVMSLAVLQINVLVQSITNLKSGKSLINISKILRICQFYQLNRDPVLYHALKDKELVQTRRIVWWQIFLLDNLVSFFLNLTPSIKLSDFDTSLLMENLDHDSCANFSIMYLNCMYRFILIVDDLNGLTNGLNFQLKNEDINKMKSNINNLFITCNSTMKKMSAIFYSIPNNSEMLSTDNNSSSNPSPSNQYQHRPISRQQQHQSPLSNSVETDPTLNSTSYSFHEPSLDTFRFFMSSLNILSDKMLIMLQKKILLSPYLIYDTHKTNSNLNLRLNSTEYSYTDLQNNLLPSLLHYLDTFLLLSKKDMLKFNWKLKNYIPIDELILLMQILATNYKSGIFQNEIDKFSDINLKIYLIDQTINSLKLNWHLKLSSVNKLISLTSKLWELMILKFNIDLALSYSMSDKYIFPKPVHEVLSPGNYSRNINISSNSVSCAEIAPRTIGIASALDTPLRNMDSKQKGTMSNDTPDQNPFNYLNPSNNEKQSYNKQKTNDAIRPSCSIVDMNLNIKERFFSVAKLVEDELSKEGGSGIIQDTDDNEEGWFNLGTEEEYGLNSIDDFHFYKNLKSDVIRLFKLVVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLG5</accession>
    <name>A0A1E3NLG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018029.1">
        <property type="nucleotide sequence ID" value="XM_019161319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLG5"/>
    <dbReference type="GeneID" id="30178006"/>
    <dbReference type="OrthoDB" id="15108at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39476:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39476">
        <property type="entry name" value="NADH:UBIQUINONE OXIDOREDUCTASE 6.6KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9735" checksum="0C3BA3EB4CFEDDF1" modified="2017-01-18" version="1">MAGHFGRHLLHPDKEVEKFSEFRENLIKHYKWNLRSAGFAVLFLAAIPVGLTYVGYKYQSLHLVAAKREEPIFEKHNNWVPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLM2</accession>
    <name>A0A1E3NLM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72328</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017356.1">
        <property type="nucleotide sequence ID" value="XM_019164159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLM2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLM2"/>
    <dbReference type="GeneID" id="30180846"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF295">
        <property type="entry name" value="AZ2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="561"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="294"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="477"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="128"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="338"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="505"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="685" mass="74840" checksum="9B40D1050B5D47C2" modified="2017-01-18" version="1">MSLAATSDLTHFHLHSLQLPGDNSSTASAAAKPSDKPKFNSLHASANMGPNASAPANSTSSTPQLGINHLSMANMASMASMASMGQLNMNVNVNQQLSHLTQLGQLGQLGQLNINPLNSINFPVNNIASNSSSSPQNQTQPLANGTASASANGSSNSSVLPTILPPPLVIGKSGQSQNSKVSSASSPNSPIPKSRSLDHSQLHQINRLNSNDEIPLSNAVNLNMNQGQTGIKKSASPLESNQAQNNSNMVMLPPLATPYMYNMANGNAANGANGNSSAANGGINSSGNAAGESIMVNTNGNSNGNMSNIGKSPQLASNSSASNLPPLFRLSHDASLPHQHSNLQHQNQLQQHQQQQQQQHQQHQQHQQHQQHQHQQQQQQQQLQQQQQQQQQQQQQQQQQQQQQQQQHSMSTVFNTYNGFNSFNNYPSYTSLQHNFDSPLPFNSISNNHYITSLDSNGNANGANQIANANNNNSLHSIHPIGHANGSSGMHSIKRSSSVAGLDHNINNSTNSISSASSSSSLPAAKPAHQPRKKRECPICHNFFSNLTTHKTIHNKDSKPFICSTCNRAFKRLNDLIRHEKCHLSQLGEWEFQCPYHPGADLLRSNRGEICHHTGFFTRCDTYKNHLKAIHFRYPPNTLKSERLRVSGHCRECGGYFDNVQEWLSKHIETNQCPKMINPSERKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLX1</accession>
    <name>A0A1E3NLX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Glycosyltransferase family 71 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15065</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018179.1">
        <property type="nucleotide sequence ID" value="XM_019160647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLX1"/>
    <dbReference type="GeneID" id="30177334"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="754"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="773"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="95888" checksum="48ADE9F2C057C1B2" modified="2017-01-18" version="1">MILARNKYVWRLYNLFRKYRRFIVFTLFAFISVNTFLYTFPSAPEFPEIYEGTFRGKYLKTIRSADDLKTDNFYTKWSLIGYLDDLVNEPIQNQTFHNSVDPFSVLDSYETNNESFVYQQLPLWDRINFYADTILPESNFTFRAIQDIIYKDAYYSKKFINTRLKKWLKIKEHLTEKEQEQLDINDDWFENVVIQLREKKLEHNQISATEIKHSLRHFKLFSNLFLKTENDAELINSPQAPEYAKQCSAISKKLFPWLTGETPKFRSYDKKLVEKKIDPYSDANSQLANNCFIKMLQSKMKGRGIVFTTNDSLVPELAAILSILRLTGNTYPIQIFHNNDLSIESIKIINNIANDPIMKLPKSIPEFKVPKKPVTLDITYVDVSNTISFTFKKYYSHFGMKLLAYLFNSFEEMIMLDTDTVIVEPLETFFSSPEYLQNHAYFFKDRDANSFLYEGIVDYFKSYLNYDHEMHYLGLPKVDDKVLNNRFFGGMARHFMESGLFVINKKEKFDGVVASLLLQMFKLFSGSLHGEKEFIWLGQEIMGQPYAFNSNPAIAIGELSPDKGLVSHELCSTHPAHITKDYNLLWFNSGFLTCKKPESYYKDINYERNQQKSLIELKKEYLSPLHITHGLIPPPAEFSVNMKNGEPTRGWAMTSECSNYLWCSYDIIGGGKNPSIPQGSVISFHENDIKKWDYLGRLWVNYFNIGYLGSSDHGYIEGDAYDELGLDDLDVFKMKHDEEDEDVDLSLLDDSGLDGISGDELPSESESDFTSYVEREKKKPDLSRGKANMKPKIIDDDQDDSDVLSIVDEEHGITKEFKEDFGGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM35</accession>
    <name>A0A1E3NM35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transport and Golgi organization protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018312.1">
        <property type="nucleotide sequence ID" value="XM_019163885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM35"/>
    <dbReference type="GeneID" id="30180572"/>
    <dbReference type="OrthoDB" id="191601at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="34253" checksum="73335B365CBD25D9" modified="2017-01-18" version="1">MCILLCTVSHPDYAFVLGSNRDEFFSRPTQKAEFIDGRYLMPVDLAREEHGTWIGVSRGGRFCALVNYREDVYPCQMGAISRGRISRDFLESELEPVEWAEDIRRRTDNFANIGGFSLLFGVLNSSNCENSLYVISNRVEGLVRPFADEKGDYRVLGLSNSSIFQPWPKVWRGKELFNTMLSQRTVQDKDAMIESVLDIMCDQYDGLQNGSSMRDNIIDNVPQSVFVPALENDLPNDLERRQGHYYGTRTQTVILLTHDGQLTYVERDIRVGDGNITESLHPQGKASDYYPMSRFDFPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM57</accession>
    <name>A0A1E3NM57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15189</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018320.1">
        <property type="nucleotide sequence ID" value="XM_019160707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM57"/>
    <dbReference type="STRING" id="763406.A0A1E3NM57"/>
    <dbReference type="GeneID" id="30177394"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF51">
        <property type="entry name" value="SNF2-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="958"/>
            <end position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1248" mass="144005" checksum="31C2D2D4A3090538" modified="2017-01-18" version="1">MSSVDPKLKQIVEITNNLENFIPYATFFVTQWPPFPSPGMLSPDGDGWRWLDFPYKEIDGLSPILRSFRLLVRKAYCRLTYKVFDKCPQILVVRAYLFPLSSAHSEIIEYYREHFFRFKRPVCWLEKRCLLILENLLTLADFSAQAFNLNTEQDLVSYLETHKIALINAMVKSDSNVEELKKVTSEILNFHLERIVEGRSLEIQNFHSANSALHQSFLNRLNRIYANIQSPAMVNQSFQDEDMRRVYDKIENDEIDGFKSQLYNFQKRSVMKMLERESFQTPQLMPNIIEIKKGPKKGKVFYNISHLLPTLDPPFYLTPKGGILAENMGLGKTCICLALICISKFQISEIPDTYRDMKLHDTNYNSSKSEGVRSLFDICIGFVAQNSIPWKEYYDDFPSNITKRLESTIGYFQKVESRTGLRKTRSSLHAENCKTSSSYKKLYLSSTTLIVLPDNLFHQWRVEIFKHVEKNFLNVLEISTSEQSNVLKAAHIRELLSNDIILISNSVFSKQFESANSVLRNVFWKRLIIDEGHSMNSKTSRAVLLTKDLMYERMWAITGTPTSGLTNLHIEDDSQEYSIQKQFDPKQDLMKVGSMVSSFFKIKPWASNGKLWTNTIVKPFERNEFSIDLQIAKFLEQLIVKHAISDVESDIKLPVLHHKPIFLDPSHFDKLSINLFIAVLATNAVTSERKGVDFMFDPTNKNDLRRLVSNLQKGTFYWTGFSINDVENLLNICVYSLRENQVKYAKEDRKLLQNSIFISKLALSDMRWRSVTTVHEMGYYVSSLPRAIVEDFSLVRYRDNISVYGYPHLISVQKFFYRNRIIKNNEDLQVKVQKQSSEFWAGYWKNMKGDTKGKGVKTTRALGNKEDYKAFELSDVQEVSHLPSWVETFNPLLEEALLYETSSGNVGSLGNRPAEPDSKSKVNGSFRISDKFNVGSNMRNATIVGTMSSKLNYLTMRLLENQIQSFKSIVFYEFENSAYYLTEFLDLLGMNYLMYSSYIKISDRSNNLAQFDRWDPGKNEGEGMALIMDLKLASHGLTIIAATHVFFINPVWNQSIEAQAIKRAHRIGQMKEVFVETLILENTLEEEMYRMRDDVKDSSSLELIDHSRIKDYIMKFPFLKMYADGKFEDEYAKLTVSTGITGDLASPTEGSNIDDGIELKHATSEYIPDKISRKWTLPLFTKHNLNKLSMSEKQGIKRRFDNIEDLENEAIVDTEGGTLGGDIGDRSHTSVLFDKLRSKRKGKVRFMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM58</accession>
    <name>A0A1E3NM58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16320</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017546.1">
        <property type="nucleotide sequence ID" value="XM_019161164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM58"/>
    <dbReference type="STRING" id="763406.A0A1E3NM58"/>
    <dbReference type="GeneID" id="30177851"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000095">
        <property type="entry name" value="Triglyceride lipase-cholesterol esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133421" description="AB hydrolase-1 domain-containing protein" evidence="12">
        <location>
            <begin position="23"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="13">
        <location>
            <begin position="126"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="449" mass="50660" checksum="0042B615DC899449" modified="2017-01-18" version="1">MSVLSSLLNIFALIIQLPLLLFHKLGIEQYLSTFHPIFNKPDNISTSSSFSFYSNSNPSSTSVINDETDPLEKLQQKIITAIDIVDIVHAHGYKVHEHIVQTKDGYLLSIHRILPPSGDIPQSSKIVYMHHGLLTNSELFLLGENTNRCLPFLLVNLGYDVWLGNNRGNKYSRKHLILSSTDEKFWDYSLDEFAMFDIPNTIDYILQITKQQNLTYIGFSQGSAQCLAALSLNHDLNEKINLFIGLSPAMIPHGLNNPICSFLVSSAPALMYRIFGKRAILPSVVFWQKLMGPKFYQEVVDKSLVFLFNWKSGNIHPSQKKVGYPHMFSPSSVKSVVHWFQIIDSKRFQMFDEGGASGSKLVYLSGASTKTNRVASFPTQTITTPCYLIYGESDMLIDIEKTVQNLSCDVETLGLKGYEHMDTLWSTNVEEAVFNKIINKLKQIDTKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM79</accession>
    <name>A0A1E3NM79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018324.1">
        <property type="nucleotide sequence ID" value="XM_019160709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM79"/>
    <dbReference type="STRING" id="763406.A0A1E3NM79"/>
    <dbReference type="GeneID" id="30177396"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043224">
        <property type="term" value="C:nuclear SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010828">
        <property type="term" value="P:positive regulation of D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cullin family profile" evidence="6">
        <location>
            <begin position="429"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="788" mass="91805" checksum="BD41099DE0E219E1" modified="2017-01-18" version="1">MEDTFLESTWESIEPSLHIILGDDKNSSLDSLMYTNTYTKVYNYCTASSKRSHRNSIGGGSNSNSTVRLVGSDLYERIKEFLKDYLLNAKQRDNESFVQYYIRSWQRYLIGSKRLNDVMDYLNRYWVTKERSSGHRDVYDIFSLCLLSWRDYKFHPNLSILMNEIMEQIQFNRQGVATTIKDLDIAIKSFVLLGFDANDLKKQNLSVYINDFEKRFLTDTHNFYALESAHYINANGVVNYINKALQRIDEEMKNLEDLNDHTRKPLNDVLNNVLIQDHQEKIRNELPNLLDQERYDDIKKVNILLKRVPLTLPPLLEVFQKYIENQGAEEIVQFKKKADEEFNLLNESYLSSINNSANKSSKVKVKKPIGSIDPNLYIKSLLKVYKQYKTVVQVSFDNNAAFTKALDSASQKFINYNIIATPTSRSKSKTPELLAKYCDEYLRGKSDDMNIDEIITIFKFLEDKESFEIWYRRCLSKRLLFNNSSPEDEENEELIIQRLKTANSVEYTNKITNMFNDIRVSKNLGVAYKDLVSKKSIDSKECVGDLEPKILDTASWGSIFRNNNESFILPAELIPTEELFTRLYKERHNGRQLNWVWNRSKIEIKANISKPGKPPYLFTVSLFQYAILSCFQEEDSLSAAYLLEKTALPAEVFKNHMVPFIKNKLIIQNPSGEKNLLKSTTMFTIVTEYTSKKLKVNFSAVKQVDARTEDKETNEEIERRKHELLKAGIVRIMKARKHLKHENLITEVYQIIDRFKPTVSEIKKAIEVLLDEQYLARDDDGTGYNYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMG4</accession>
    <name>A0A1E3NMG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Adenylosuccinate synthetase</fullName>
            <shortName evidence="11">AMPSase</shortName>
            <shortName evidence="11">AdSS</shortName>
            <ecNumber evidence="11 13">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_33164</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11 13">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11 13">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 13">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="11 13"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017686.1">
        <property type="nucleotide sequence ID" value="XM_019162803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMG4"/>
    <dbReference type="GeneID" id="30179490"/>
    <dbReference type="OrthoDB" id="10265645at2759"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 11 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="11"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="301"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="333"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="415"/>
            <end position="417"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="429" mass="47867" checksum="4DF91682C8A4B213" modified="2017-01-18" version="1">MADVVLGAQWGDEGKGKLVDLLCDDIEVCARCQGGNNAGHTIVVGKTKYDFHMLPSGLVNPKCLNLVGSGVVVHLPSFFDELAKIENQGLKCRDRLFLSSRAHLVFDFHQRVDKLREAELSNKKKSIGTTGKGIGPTYSTKASRSGIRVHHLVSDEPDAWPEFETRLRRLFATRQQRYGEFEYDIEGELARYKVLREEIKPFVVDSVEFMHSALAENKKILVEGANALMLDLDFGTYPYVTSSNTGIGGVLTGLGIPPTAIRNVYGVVKAYTTRVGEGPFPTEQLNEVGEKLQDIGFEVGVTTGRKRRCGWLDLVVLKYSTWINGYTSLNITKLDVLDTFKEIKVAVGYKYNGKELKSFPECLHVLGKVEVEYKTLPGWEQDITAVKTYSELPENAKAYLKYIEDYLKVPVQWVGTGPARESMLVKATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMM6</accession>
    <name>A0A1E3NMM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein FMP42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018464.1">
        <property type="nucleotide sequence ID" value="XM_019160760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMM6"/>
    <dbReference type="STRING" id="763406.A0A1E3NMM6"/>
    <dbReference type="GeneID" id="30177447"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052599">
        <property type="entry name" value="SLC43A_AATransporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772:SF2">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="58999" checksum="71B3053114B0B552" modified="2017-01-18" version="1">MSDTETTPLILPTKASSHLPPLGVRVAQVACSMLWCLFAAGPVFGFAALKPVLIAQGVYKEVCDIKVPPSTDFSILDAACVDQDLKLNLMFTLSAVVTNATALLVGSILDNYGPRITGILGSFFLALASVLLAYGKSIVWFDAYLVGYVTLAFSGPFVFISCFQLANSFPGRSGLILALLTGCFDSSSALFLFYRIAFQNNIVENLTLKKFFSFYLIVPIFIFLCQVFIMPHESYKTIEDIAKISETGLDERGLPIDPEDPRYLSGEVASLEQSRSRRGSMKSTKSVFEEIADHNLKEKSGGVFGVLHSKTIKEQFKSPWWFIMCLFTTIQMIRINFFVATISSQMEYYFDAKTAVTINKFFDIALPLGGIVAIPFIGLILDNLHTLVVLTILLGVSTAIGVFGMLSIKYLQYLGILLLVVYRPFYYTAVSDYCVKVFGYSTFGTVYGAIIFFSGMMNLLQTVLDNATHFTFHNNPTPVNSILVTLTVFFGLSMLFFIKSQEKNVIRQNVIDEALSGETDDDGAVNSNSFSALPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMM8</accession>
    <name>A0A1E3NMM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UmuC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_19467</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018498.1">
        <property type="nucleotide sequence ID" value="XM_019162121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NMM8"/>
    <dbReference type="GeneID" id="30178808"/>
    <dbReference type="OrthoDB" id="5723at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000008">
        <property type="entry name" value="DNA polymerase eta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="9">
        <location>
            <begin position="25"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="421" mass="47765" checksum="5B65B6EE9F747CFA" modified="2017-01-18" version="1" fragment="single">SEFTYKNLLDLNDSRKAYQSPLAVICHIDLNAFFAQCEQLRLGLSEQDPVVCVQWNTLIAVSYAARDYGITRMDRLEQAKQKCPNLIPAHTAVFKKGEPFWKYVDHMPSPATHKVSLDPYRREGKKVLNIFRSECDLVEKASVDESFMDLGRLVFRRILTLFPNILEGMTSKSDTLKPIDHLPQGIDFKGYIIAKKDDAENESAIADEDSSFLVEDWDDLVILVGSMICYELRKKVEEQLGYKTSGGVGKVKTIAKLASGFKKPDQQTIVRNDSVPGFLKYFRLTDFWSMGGKTGSYVKETLGEDISISSIRDQYSSPTELAQALDKNYDLASKIFLMIRGEYALPLEERELLKSMAANKNFRQSIYKKADLIPWINVFIGELILRIQDLDDEYNTISRPTKMTLSVLGSSGTRHSKQCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMQ1</accession>
    <name>A0A1E3NMQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_58916</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017776.1">
        <property type="nucleotide sequence ID" value="XM_019163329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMQ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMQ1"/>
    <dbReference type="GeneID" id="30180016"/>
    <dbReference type="OrthoDB" id="26399at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <keyword evidence="6 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="10">
        <location>
            <begin position="99"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="11">
        <location>
            <begin position="336"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1099" mass="124890" checksum="9D10C01F3B2B31A6" modified="2017-01-18" version="1">MSAQIQQAVEIANSPSSDQILKEQALAFITQIKTSAEGWKSCLKLLQLDSTSLHTKFFAFQVVDDRLPFLPNEDKITIKEVIFTYLDSLIANNKIEPFFLRNALSKTLGLIFVHCTTNCYSTLIKDLLSLSYNSTTDSFNEIATDYYIRTLLVIHQEIGDQMITRDSRSQDHNNLLKDSIRANDMIDLANIWKKILSKLTAVSSPSDKIVDEIVNNTILCIGYYISWIEINLILDEQYMSFLYQFLSSNDNSKRRIVTANTFNEILHKKMPSAKKLELLNFLNLGTMLSQMNLNSKIMEFDVAMALAKLINQIGVEAIIVLDNASPQELLNDEFRSIACSKILEIFPLIFEFLSNEFDDISLEVFPFIGNFLSFLKKNITNDTVNFSSLNNDELLSTLSKNIIMKLKFDPDDDGDDDETIEQFNEIRNKLNVFLDSIVLLNESLALEVLIGCINEFLFNISTGASGAGGEKDWRTIELGLYVLTYYSDMLRNNIMNLPKTMINNSRPYFVFNQMLCKVINNSGEILISHPLIQLLFFELLLKHYPFFTNNNIQVEGVNKEDTLMKVLNVFISNFGVFSANEKVKHRSWYLLYRFLKLTKPKINDYIIEELIKSLLPLLNLNFEILSNKNKAVSSTDVASLHDLDLSLIEETGSFENQLYLFESVGLLITFVSDEDSKITIFENVLQPLFSNLESGITSITSYKLDLNVLIQVHHSLISMGNILKGFENLRSETLNNIKLLNIFDQISQVIMITMENFIDFNIVRESIQFCVVRLYIILNNNNNNNTELLQSILSKFISCVMINFDKLKMSEIINLINFISQILHQSNNNENSYLLLQSLLAPFLSKIFFRIEAVTASATDDFSKKEVLDLQRSVLSILIAISNDHLNSLWLSTEENKEMLVNTVKMSLNYSSNYSVTDLSVVKLSLIVLNMLCQGIGSGIVIDPLDNYRNESNKFEQVDEILINNSILLSVELGLKVPAANKHLLKDAQFRNNVTLETCRLLKGIAHIGYEYPDANTLQKKKTEATATATTATTIGNKDVGKQQLLPSGFNEFRCQQISSVLSNNFGFPGDLSNELVQALVSNTDRQFMKYLVALVEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMR4</accession>
    <name>A0A1E3NMR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier protein LEU5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_31770</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018537.1">
        <property type="nucleotide sequence ID" value="XM_019162719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMR4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMR4"/>
    <dbReference type="GeneID" id="30179406"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000151">
        <property type="entry name" value="LEU5p Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002167">
        <property type="entry name" value="GDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00928">
        <property type="entry name" value="GRAVESDC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="54"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="153"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="294"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ47424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43346" checksum="E0E4BACB2D238B04" modified="2017-01-18" version="1">MQAERAVQKSRATDVVANVTPIATKPEKNSSNAHSTLLNKQSQEHAQINKQSFEYVLRSGLAGGLAGISAKSLIAPLDRVKILFQTNNPHYKQYVGSFKGMFKAIQTIYSRDGILGLYQGHSATLLRIFPYAAIKFVCYEQIRTILIPNDEYETAGRRLLAGSLAGVTSVYFTYPLDLIRVRLAFETSHHSIHHGKGKLFHTLKLIYNEKPKTMLLYRSRVADYFPCVLSLSNFYRGFVPTILGMIPYAGVSFYSHDILHDFFRNKWLAPYTVMSDKSKSATRSDSSFDHRPPLTTWAQLLAGGGAGMLAQASAYPFEVIRRRMQVGGVTNKSGEFFGLAHTAKQIFKERGFRGFYVGLGIGFLKVVPMFACSFYVYERCKELLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMS1</accession>
    <name>A0A1E3NMS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017796.1">
        <property type="nucleotide sequence ID" value="XM_019161236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMS1"/>
    <dbReference type="GeneID" id="30177923"/>
    <dbReference type="OrthoDB" id="3996280at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF16">
        <property type="entry name" value="UBZ4-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="750"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="773"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="810" mass="88632" checksum="CC600CAA98069C9E" modified="2017-01-18" version="1">MATNFNWNHIPGVQNATGSVAADTAPIDHFDAFFNSQLGQSPQPHSASALPTDPMSLHYTQPQSQSQSQSHPQPQQPQPQQQGLTHYPQSSATNTQAQPPSGGYLQHNNAVHSDSSLSNVFNKLNISTTKSHSDDTIDTIKLGASKGANDSSSPLKKIMENPTHSSSTDTIIADNDSSIPLSLALNDMSQKELKTYLRWYENIQERKITNKTVTLEDVFLFMQNFRIPQQIKERMKMVFTKWSNSLNIGQFFALMRLLAHALLGEPLARSLIKVPAPVPRPISILSRKRKDRSTASSSDTPVLAAPEEPKKKLDIDSFTEFILTGERPSLKHTSSSTKSHKKVKFSEVVSFSPPPQSGEPGPQPQPEIPMMMHLAQQHVLDFSLPMDQLLDRLKAESTGTPEAVSQSQTQSQLQQQPLKPQPTENEEEVLKDVQMDTFKNITQKATRNTADEPLKPNLTGSASKSMKEHFLQQFDQTFNFNAGPFDSTYPNISALSAVPAVSSPSTDQLPIMSPMKETPPVPQSSYNTPQLQMQPPLPNASQAKVQSPDRQQTDYFGMVPQNGASGSSPMISSLAAGMGVNNTFQPPVPPRSRSRAGSSASPPPIPPPPPPPRAIKSSRAATPATTQSNPALPPKPVLNEHQKQQYLSASEGDNLMQLNPLNGMANMNANGLAAVNTGYGNSYNLNGMKSMSQVNQLNQITQMHQLGSMNSMNGLNSLNGMASPNINKMNVSNVQGYNRPPQIVYGQQGTMGQSPQFQNQGMFMSQQTGGGWPSLHQQQPQQQPPTSQLQPQQTSSHPQQQQNAWSGWQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMT8</accession>
    <name>A0A1E3NMT8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018558.1">
        <property type="nucleotide sequence ID" value="XM_019160806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMT8"/>
    <dbReference type="GeneID" id="30177493"/>
    <dbReference type="OrthoDB" id="4079943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="89" mass="9950" checksum="25F5D1C3E1E26CDF" modified="2017-01-18" version="1">MQLMGNNGLQSLFKFYSRLNQLPSQGAVSGVLASENNAISVHYTLTKGDEVPPQSGYDYDLFYNYEVQFGEIEVKNGYLMDCVENSTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN17</accession>
    <name>A0A1E3NN17_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018612.1">
        <property type="nucleotide sequence ID" value="XM_019160826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN17"/>
    <dbReference type="STRING" id="763406.A0A1E3NN17"/>
    <dbReference type="GeneID" id="30177513"/>
    <dbReference type="OrthoDB" id="10249045at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000964">
        <property type="term" value="P:mitochondrial RNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013943">
        <property type="entry name" value="Pet127"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014:SF0">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08634">
        <property type="entry name" value="Pet127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="756" mass="86483" checksum="9339FC12C213E142" modified="2017-01-18" version="1">MLSYRISTRGVCVFPPLLSPRCHICNNSSLAHNQRPQDDPSDIRAQLQYQRNSDVIKNLNILAEEDNTEDDVEPTTCISKGTLKRTNVPEYISLTHFNDSVSKVPELSHNLASTLFSPGVHYMTDPRTNHFNFDKSLTNIPHIDTLKMDKISHFTPSSRDTKLLDIANTLNKRRLKKHPHKKAPAVKFFSSTSSMTSVLIKFHKYLSHNRPINTTSFSKFYPDATQFTRTSDVPTSLVVTPKDDDKQIYSLDADRSTDSEITLSILGNALELMLTKPSEEFQKFLKSSKEVPKDDSSAYHYAKIGKFLVRSQLDAVDTRLPGTGTFDIKTRAVCAIRFDLSHTDYFPTNYEINKTYGLFESFERELFDAARIVMFKYSLQARLGNMDGIFMAFHNIKKFLGFQYLPLTDIDNIFFGEYSILGKKYGQKILDKTSEEANAEEVNTSNQLTLKNIVNDFGNHHQTKREVLSSFVADYELRLSMNLLEALLENIVKDTKGKPFRIIFKKIKSNMITPPEPKPVEATDTETPKENITETKTASEKLENMVKPDSTAEELASTSGAPDSIVVIVNTLEKEELNELQMLTQEKLEQSKQIVKNAQNNKSMSPSDRIKYFSRYVSDFRNKFFKLNRVILKNSEKEGFFAYQLQADHYFNGTKSEDKHPMPAIEILDKDSDYSWDVKYSINRINRIADKKYLYNKFIKNIAFTSFRDADSESNIDVYTDEGDVQLDENATALQNITRAYSAKALKRKELSKSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN56</accession>
    <name>A0A1E3NN56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15472</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018653.1">
        <property type="nucleotide sequence ID" value="XM_019160836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN56"/>
    <dbReference type="STRING" id="763406.A0A1E3NN56"/>
    <dbReference type="GeneID" id="30177523"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052599">
        <property type="entry name" value="SLC43A_AATransporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772:SF2">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="61692" checksum="F6115BE78A56A74B" modified="2017-01-18" version="1">MYFFETSERKRYAQVACAVVWCLFGAGPIFGFAALKPTLIDQGIYESVCDVTNQTDVSIAAHAPGLFTELLTSLSPVRADQLHTEGFVSKCTDQDLKLNMMFTVGAALTNVSALVIGRTLDSYGPRVCGLIGAFFLLLASVIFIYSKAILSLGIFDPYLVGYSLMALGGPFSYISSFQLSNAFPEKSGLILALLTGAFDTSSAVFLIYKKIYLSFPTFFTLETFYKFYLAVPLFIAVVQLLVMPVESYLTPPPELIPNPTDEQRALSIAAAQEEYIHNGMSQDHADDNDNTPLLPPTSTRRRSSLGDAYKSIYIEEEVEDATKSKTELSYSIFGILHSYSISYQFNSWWFKLICLFSTIQMLRLNYFVATVNSQYTYLLGSYQSANALNRLFDIALPLGGVVSIPFVGMFLDHFPTSIVLGALLGVSLLIGVLGVIKNSFLAGAVNVLIFVAYRPFFYTSISDYCAKVFGFETFGTVYGSIMTVAGLINFLQSVLDRLTHTKFNMNPIPLNLLLLAITVVIGTATVAYVTVQTHKYALMKFKGNTSSDDEETEAGNHDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN68</accession>
    <name>A0A1E3NN68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_20006</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018669.1">
        <property type="nucleotide sequence ID" value="XM_019162149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN68"/>
    <dbReference type="STRING" id="763406.A0A1E3NN68"/>
    <dbReference type="GeneID" id="30178836"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000001">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="78" mass="8761" checksum="57F926F9ADA611B1" modified="2017-01-18" version="1" fragment="single">MGKRKKSSRGPVKKLKQKLDTQFTCLFCNHEKAINCTIDKKSNIGTLTCKICGQSFQTSITSLSEPIDIYSTWIDACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN76</accession>
    <name>A0A1E3NN76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_31209</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018201.1">
        <property type="nucleotide sequence ID" value="XM_019162682.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NN76"/>
    <dbReference type="GeneID" id="30179369"/>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02666">
        <property type="entry name" value="Peptidase_C19J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025305">
        <property type="entry name" value="UCH_repeat_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13446">
        <property type="entry name" value="RPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="744"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="890"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1199"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="929"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1346" mass="154891" checksum="C7FB9051DCB77AA4" modified="2017-01-18" version="1">MNNSFQPKHNLQNSENNVPKIPKLDDDYQIEEEDGDVLGHNSDRESQKGATDQGDSYLQQKQSTEGSTHPYNSLSTGTNFKPTSPVDQPVKKKYDFSKAICMKTSDRIFDDLRSDPLFLIKGEEGILSTPTARYSTTLSKLSKTTGYKYQPAFWSTNDELLGSSQITELDGNTTLQKFSGLLVPNHHSGSDFSNLQLFHYSISIRYSNNRLNVKNSFYGFLDNDLFEEDRIWIEPTDIMRDTDSTSDEARQILIKSLPQFLDSATFISKETGTLIRIEIYPAFFQGNDLESFTLTEIQTRIATYNSQVDRSESFSNVDITPYDCFIKLKKALSGALAHQDIKDVKSIDLKATKLQVMIDLQLLLDKFFFILTENDNLGQLTPIHFASLGDYFFVTQDYFKRAILETVYYIAHTSANRNDFSNYFSYSFHDIFHCVKEFDITNQIQNWNNWNDQYYSDPMILLSICPFYGDQLICHIYELLIAFDPQNIPLYFDALTYCATSRYSEELPYYVTILKGRGQLGFGEMKNIFAKLGFNNATSCIAMSAISDEQLLNAYRNQLIVAATKYEKVTFREMLDKIATSRSSDLLREYLNTEPFFDIYDAYKLLDIDPSFDDSLLITFYDYKASENGLNFDPNVARAFYTIVLARKSILLMSYIDTNLPQFSVYDMSLHDAYQFVGCMNTADDLVVVRVFQERLHKDTNIDFRMLWKSLKTIGEHRKSKLIEGYLTSGIVNSTLLSTEQSPAGLNNIGNTCYLNSLLQYYFVIEQLKDYILNFNEVFSAEDFEKDEKYQIRRIGGRTVGLKETERSYQFMYQLRDLYYQLIHENDKCVTPSRELAYLAFSPISFEVEFEIESNEKDDSSKNLDADKLLTTAAEDDESKKLIEMSLNEFYKGEGPNEDSKPVDDQVIDLTNDDVNSNGDGKKSNSKLTTDTDNADNSTSDDGSILAPDELPKEPEKKKTAAVCKISADQFESAFEIGSQQDVTECISNVLTQIESAMKPDILEEDNEQVDMVKKLFYGKTKQRLVPVDSRTKEELLNGNVRTKVESFLNLIVNIGDHPKDIYDALDTFFTEDLLELEDGEVKRSLTIIELPNILQIQIQRVQFDRERLIPVKSNEPIPFEEKLYMDRYLETSDQAIIDKRTELFHWKRRVQELNQKKQEIMSTNEQGMSIRDVLKATRSYLSSESVKDLGIPIDMNTLEVLDLEIDRLENVLLGINNELDILHNNINKQFKGFNKVGYSIFAIFIHRGQASYGHYFIYIRDPKSNVYRKYNDEVVSEVPVEEVFNFSDGNTATPYYLTFIKDDLLEKITPLQRDIFIKENVTKLEELNKNSYVASNVDNLHMDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNB2</accession>
    <name>A0A1E3NNB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16750</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018066.1">
        <property type="nucleotide sequence ID" value="XM_019161330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNB2"/>
    <dbReference type="GeneID" id="30178017"/>
    <dbReference type="OrthoDB" id="3996389at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="584"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="56"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="594"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="352"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="646" mass="71745" checksum="0EC8CE9E09097953" modified="2017-01-18" version="1">MGLKKLFKPPKDIDDKGTKELLRKNGVVVRDDMNYHRKKFKFGEFGKFSEQQAQNLDTTLRPVLPNSQTGQANYDPAANQADSNQGYGYDEYASNFRGQGRSGQPNGASNSYSYSYSGGYSNPDYGNGNSYGRGGSNSYGYGYDDSTAYSSRTARNPSERATKARQNGDGTPQRRPKQNNYPSYSSSTSTSTSTNSYGYQPPVASSSGNSARNEVENSSNTGPGNGYGYEPSINNYSGSNGYGFDPYADTAVTANSVDAVTPLTKTKTSNTTVTQSTYDPYAPGEEPHADSAGAGTSASASASALQDSSGGYGAGYGSYEPDVNDYGEADDNATVTGEQVNGLASGMQELQLQKEQTRQTQQTQDQDFDPYGPSATLQTQTTTTTDLDLNAYEPTPEMQEQSVPYDEDEFEEDVDEDEEELNRIQRQTKDVREDTVNLSRTLVDNLGQANVTATNTLGVLGNQREKVYEMQNNIGTMKVQQRFVDDHVKDLEHYNRSLFHIKVNNPFTRNSRRKAKDQAFLAQRQADRANKDQLNSELYQSQRAIMDQLKNDNIKSELQEKYDYDRRVKEAAKYLTKDHDEEDERMEVEIAANVEHAQKLANNLKKKAMLMNSEIQQQNDDLHDVSGNVDYMDDKVVMTTRRIRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNE9</accession>
    <name>A0A1E3NNE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_116768</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018734.1">
        <property type="nucleotide sequence ID" value="XM_019159824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNE9"/>
    <dbReference type="GeneID" id="30176511"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="94" mass="10474" checksum="DD7E62A6D572482E" modified="2017-01-18" version="1">MDRYGCGRARGALYRGGEQRSKVTMYKTLVQRGRLWLSVALLLLLCRHPHTAAATVLFRRTNSHQVSTLSTSSASSEGCIARPPVEKKTMIVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNF0</accession>
    <name>A0A1E3NNF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018748.1">
        <property type="nucleotide sequence ID" value="XM_019163980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NNF0"/>
    <dbReference type="GeneID" id="30180667"/>
    <dbReference type="OrthoDB" id="10005898at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="304" mass="35179" checksum="8FD95864A91EB835" modified="2017-01-18" version="1">MQIEHRPTHNLRSIQQRVKFLSSLIYDPKFYRIINTVISDPSKLDCTLGTLCYFTLLVGAVLNRYPQWKVVLQKLWSRILSLRHKLLSKVWGKTQPKPSSEPLKTPEAETEKVCKYEESQKSMAEGLERMEKILKQFSGYITDIRIFNRGFSIPSCIADVLEAGSLLQKKDYLGFVSTWCISLYQPLETIAFLFDHNWLLPGREGNNCNWWYAISTRFWFAWVVAEFSQLTYKLLIMKRGKNVDKEELIAFIEHLATLPLCVHWSLEDGCLDDLKVGLLGTIAGGLSTFDIWRGTWGTILKECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNU4</accession>
    <name>A0A1E3NNU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartokinase</fullName>
            <ecNumber evidence="10">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71788</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + ATP = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:23777"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018869.1">
        <property type="nucleotide sequence ID" value="XM_019164007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNU4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNU4"/>
    <dbReference type="GeneID" id="30180694"/>
    <dbReference type="OrthoDB" id="4323675at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000023">
        <property type="entry name" value="Probable aspartokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF59">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="11">
        <location>
            <begin position="448"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="541" mass="59487" checksum="14204C0DB0107364" modified="2017-01-18" version="1">MSSASLYSLNSGWVVQKFGGTSVGKFSKEIAVDIVKKYNQSNQIAVVCSARSTQTKSEGTTTRLLLAADLASDDKEAEFKHTLKLIKDDHIANAETNIKDQALREDLIEDTINEINSVERLLDACCVIGEITPRSLDNIMSCGEKLSCLFMTALLNDNNVPAHYINLSNIINELDYNNNSQLTNNERSLDPFFYKQLAKRMADAILTLEPGVVPVLTGFFGPVPGGLLNSVGRGYTDLCAAVCAVGLAANELQIWKEVDGIFTADPRKVKSARLLKSVTPDEAAELTYYGSEVIHPFTMEQVINARIPIRIKNVMNPNGEGTIIYPDNIGKKGEDTPPHPPVSLDTLPTDYFNKQKLKIPTAVTAKSDIAVVNIHSNKKTLSHGFLANVFGTLDRHKTVVDLISTSEVHVSMAVNYFKDKRKSFLVVIEDLKRFGNVDVTYDLCIVSLVGIHMKQLIGTAGAMFKILADERINIEMISQGANEINISCVIGQDNVIKALNALHDKLLLHPFKNTNKCLSYSNDSSGMAVDHRIEDLKLSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP78</accession>
    <name>A0A1E3NP78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Anaphase-promoting complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15776</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019014.1">
        <property type="nucleotide sequence ID" value="XM_019160959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP78"/>
    <dbReference type="STRING" id="763406.A0A1E3NP78"/>
    <dbReference type="GeneID" id="30177646"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="315"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="349"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="458"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="492"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="69934" checksum="C67734C479A3177D" modified="2017-01-18" version="1">MRAAGNNNNNNNNIHNPESSTIFDINTPLTQADKLRLWRHDALLQHHYKTAIYIGDKVLSLTNDPNDAFWLAQVHYASSNYQIARNLLLGPNFEASVGCRYLAGLCLIKLEKLDEALDIIGEVNPFKKDHSIRNPDGGIKLEASMCYLRGLIYAKQNNFERARDCFKEAVLVDVKCFEAFDELISNHLLTPNEEWDLLQCLNFDDADNNNELVKMLYTTHINKYKNLNLYEDAKQRLKDEYDLGDNIDILLSEAELYFIKCKFQECLALCTKIIERDELNISALSNYLSCLYELGGKNKLFLMAHRLAENYPNHYITWVAIGIYYFAIKQISEARLFFSKASILNPNFASAWIGFAHTFAADGEHEQAISAYATASRLFPGTHLPNLFLGMQYLQMNNYTLAWEYLNYSYAICPTDPLLLNEIGVLNYHKSELQKAEVYLCRALVASKSLEYGSKAWCSIHCNLAHVYRRMSLYDKSISHFNEVLKIAKDDVNIYSTLGLINLKLGKIANAVEYLHIALSIQPNDPMATDLLDKALDLNIRVANKKFLSESLNTPNKIKKPNFHNTISNFSEVLKSLGSGSKKDGRKLEGSIPKGKNYIDIVDDEDEIMDIESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP93</accession>
    <name>A0A1E3NP93_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018476.1">
        <property type="nucleotide sequence ID" value="XM_019163924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP93"/>
    <dbReference type="STRING" id="763406.A0A1E3NP93"/>
    <dbReference type="GeneID" id="30180611"/>
    <dbReference type="OrthoDB" id="4070583at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="681"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="866"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="888"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="920"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="937"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1099" mass="118328" checksum="BD9D5CC55F888965" modified="2017-01-18" version="1">MSEPAAATTTAGSVNHQRTQLHKTVSAVSKDSKWVSPFRVSGAVGPKVKSKNPANHSSYISAGSIPVVAKPAEEEAAPAETAEAEEEAPADTTAEAAEGVANAEYDEEAPIDETEEPLEPTATTEDAAEEAEEPIGEDAAEAAAPETELAEGEEATEEEEPAAAQPVSELAAATKPKEKKTLPAMQEVPALIKSHPKMLRRYKDNFNITSQIMNKNKIENLDRRVNLGAGLILTEEQIYELARKKLEPLMKQIDERVAENNARDAAAAAKVEEGIRLNDEAVVAKELSQYKSTVDAKTKSVKADHEKKMKELADKAAKSKSDTEKHIQKQKDGIVKDKEDAEKAEEDAAQKHITDKETLIETADKLKEDKTKELEDAKAKQTEETELAEKFQTDATELQSKQVDLQSELDAKKAELEEKIKKVEALIAAKHERKETIRSSIKRRKVADRSFNIIDAKHTQAAANVAVLSNQVGLLNDRLAAHSTKIDHFNTTAKDTLTSKRAEAKKASEDWEDHLAQVRLDEAKKQEQIRIAAEEERKRVEQEKLEEEARLQKEKEEEEAQIAKEKEEKEAQLAREKEEEEAKLAKEKADAKIAAEKKKKEEEEEATRVAEEVARMKRLKELNEEKIKLQKSLDEHKKAEAAASANAKDESEKKSKSSETSSSGAGILAGVGAAMVGAAATGTAAAGSAASGVAGSIGNPLGSSTSGALPSYQEELAKSDVGNTGSFGSNNPFYLGEFNDKKEDDLPSTIHEEEEVEAALPVGKDATKLEKLATPVATKQVEKVEEDPLNDNVTITEKAASPLETAASELLVADDEEPVSEVEKYPIPAEMKPADDSALTSATRRRSMVDEAIAHMTPAELERMNTPLSQQIKNKEPTTPTTAEGRSRSNSLSKSRSNSLTSKFKFKKSGSKTKSRSNSIKNETPKQTVTAKKNNPKLAKSTTSKAAAPVSTAGTAAATSSPTVPATPTIESSSAVAPVPTTPASAVSIAPSGLSEAAEVSEAKAVEIKPSTDESPEKVASGGGEQDSSDDDDDSDLAPTTVNPVFTEKIGTTETAPTTANTEPAADASNYVEVSTLETVSSNEYKQNKDDPNYMVLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPD7</accession>
    <name>A0A1E3NPD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho termination factor N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_121188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019028.1">
        <property type="nucleotide sequence ID" value="XM_019159885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPD7"/>
    <dbReference type="STRING" id="763406.A0A1E3NPD7"/>
    <dbReference type="GeneID" id="30176572"/>
    <dbReference type="OrthoDB" id="4034134at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038872">
        <property type="entry name" value="Put_GTT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807:SF1">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36347" checksum="39D515866C838DDA" modified="2017-01-18" version="1">MSTLSKKTKGQLVEIAQDLDIDIAECKVKKDVYEVVRDYFISNSEKFDENSKYYELAVLSKFGSPKKTVFIESENGDVKEVDVAEDDDDETEEAEDNDGDADDDDEDDEDDDEDDEDFDFECYTSLVKAIQHGTVSEYLELKNYELRDYLGDPYTINELTFSIETAVLLYRLISLTTLDTYLPSSVLKYLPAFAQTFPVISTSSFNLTNAASLVLWLVSAKALPLIFSYYVNFTYDFDRDAFTESLAKLFLAIIVFKSDFTLSTVGNEIKYAFRDNAYEAVKHYAFVATLKLKDTFQNWVLINALFTSILSLYANLAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPP8</accession>
    <name>A0A1E3NPP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_72086</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019214.1">
        <property type="nucleotide sequence ID" value="XM_019164095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPP8"/>
    <dbReference type="STRING" id="763406.A0A1E3NPP8"/>
    <dbReference type="GeneID" id="30180782"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000329">
        <property type="entry name" value="GTP-binding protein rhoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="200" mass="22068" checksum="49F2552E201B1B7C" modified="2017-01-18" version="1">MAGAIADIRKKLVIVGDGACGKTCLLIVFSKGAFPELYVPTVFENYVADVEVDGRRVELALWDTAGQEDYDRLRPLSYPDSNVILICFAIDSPDTLDNVLEKWISEVLHFCQGVPIILVGCKLDLRNDPAAVQKLAQLGQQPVSTSEGQEAADRINALTYMECSAKYNQGVREVFEAATRAALRTKEKESKIKKKKCSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPP9</accession>
    <name>A0A1E3NPP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019213.1">
        <property type="nucleotide sequence ID" value="XM_019159740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPP9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPP9"/>
    <dbReference type="GeneID" id="30176427"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="57"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="391" mass="43466" checksum="A263628A41065F97" modified="2017-01-18" version="1">MKYGIEDIRSQFPLLADETVIFLNSSFQQPMNLIVSGAIERYVKDGLHEPNPKPKWNAECENVRLKTAKFINAQSEDIVFTRDTTESCNLFQRSLEFKPGDNVVLLRNDYPSQAAGWLELKSRGLEIRYIEMGESGPQPYDADAFRDVVDEHTIAIGLSSIMFDSGLRHDVRSICNTYRPRGVHVLVDATQEVGFGKIDVRELGVSALAFSVQKGLSCPTGLGVLYIDPQVLPDLKLTPPIMTPSSLNSTQGGSSVTGCKSARSLEHNNKALMQCLALGEYLDFLDTVGITKIQTHLESLSLYLRLQLKGLGVETIGPDDQKYRSPHSNVIKLHSEWLPFFVDNHVYVSQYGFGVRVSFGAYNTKSDVDMFVAVIRKGLGRGLTQTSHSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ17</accession>
    <name>A0A1E3NQ17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Pep3/Vps18/deep orange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_32350</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019312.1">
        <property type="nucleotide sequence ID" value="XM_019162761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ17"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ17"/>
    <dbReference type="GeneID" id="30179448"/>
    <dbReference type="OrthoDB" id="1845386at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007810">
        <property type="entry name" value="Pep3/Vps18_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058919">
        <property type="entry name" value="Pep3/Vps18_RING_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF26">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05131">
        <property type="entry name" value="Pep3_Vps18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26148">
        <property type="entry name" value="VPS18_RING_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pep3/Vps18 beta-propeller" evidence="9">
        <location>
            <begin position="18"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="575"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="Pep3/Vps18 RING C-terminal" evidence="10">
        <location>
            <begin position="847"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="801"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF26148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="955" mass="111140" checksum="FE4EB334ED4A6C6C" modified="2017-01-18" version="1">MPLQLDTVEVNAKNESAFDFEEVQLQFQLESEICKLLVRNNHVNLVLKTGVVHIINLDDPEVVIDIQLPLGKENQLRDAWIDSHGHHLILQSTKNEYYYINNSSATYHVLNKLKNLNITSISFFEECVGKDTTGHILLSTSNSLLLEYAISSNKEVFLKTILKNKYCITHILNTATSHESGIASYSINLFTTENSILNLKVKVPLEPTSNVSVFQSLAKIEPLIIKHGGISNISNDMKYLAYSESSSNGSKIHIAEADIKNQKSFNPSSIVLEHLRINTFILTGYFILVLSANNHLEIYNQLNLKHLKSISLSYLDQKMQGISFDRLARTFWLYSDRHLYELIVNFKTSGIFTTMIQKHMFDDALSLLPDDKSAETRRRKNYILKKKGYYFLEEHNYKEAVKLLAQTDESFDKVALKLFDLPDKAILRYYLTVKLRCLPNSMKSQKKILTSWIVEIFIEQLNTLETKILHSHKKNTILYTFFSENLSSFDKETIYQIIIVHNRKEDLLHFAKLINDFQFVLKYYITLQMWNESLNVLASQQDPELVYKCATVLLVNHPVKTIDTWVRLIDDLSELKLIPSLLTYNKTVAFPQQISPEHNQSLRFLKFLIYEKNVSNRIVHNTFFSILITYPNTHNENLILKQLESYQSFRKKKFRKFEKGETFFDYDYILRLSFKFHKIQSAIFIYSILGKDEEAVDLALDNDLIDAAILVADKPNEKDEYQRKELWLKISEKLISKVVVNKEYIKQHQNMFYDDRVPTAEEENEDPVYILLRFLTEKCDALAIKDLLPLFPEFIVIDNFKESLIESLKKLSLEMNKAAVEMDNTLNESDKINQKIKDFQSTNFQIIEPFESCQLCHKILAIRKFIVFPCSHAFHQDCMVKNILESNDYKAKNSIYKIQKKILMNNKNASVIEDLKLEIDHLLSRSCCLCSDMKINELDEPFIKSGDMERDGWNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ91</accession>
    <name>A0A1E3NQ91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aminodeoxychorismate synthase</fullName>
            <ecNumber evidence="4">2.6.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Para-aminobenzoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">p-aminobenzoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_32193</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11672"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58406"/>
            <dbReference type="EC" id="2.6.1.85"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.85" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019390.1">
        <property type="nucleotide sequence ID" value="XM_019162750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ91"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ91"/>
    <dbReference type="GeneID" id="30179437"/>
    <dbReference type="OrthoDB" id="64220at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00149"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046820">
        <property type="term" value="F:4-amino-4-deoxychorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008153">
        <property type="term" value="P:4-aminobenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010117">
        <property type="entry name" value="PabB_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01823">
        <property type="entry name" value="PabB-fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF18">
        <property type="entry name" value="AMINODEOXYCHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="12">
        <location>
            <begin position="268"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="11">
        <location>
            <begin position="451"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="724" mass="81843" checksum="A1C260BECE9BB5F8" modified="2017-01-18" version="1">MSILLVDSYDSFTYNLRDLLLQSTNAHVITVHNDSYDLSSESDKTELDILLRSVDAIVIGPGPGSPSVASDIGIIPYVFEKCQSKPILGICLGFQILCLVNGCNVDYLKDPIHGQVHKIDIVDASSIFQNFPSSVDSVRYHSIYVSDITPSDSIKALAYYTDHKPGSSKVLMAAQHTKYPHFGVQYHPESICSTFGKDLIQNFWKLVEATPSNDILPVLSHDKHLVTSSPLISEFKSTNDYNISYKTLTLDAFSNGQIDVLDLCDLLIAQKNDIMLLNSASSPGEWSIIALPTIGESEVITHSTENADVVKLSKWKSDSLPSTIHTNDMFEYLADYMKNKFYNPKMENIELKSCPFVGGLVGYVSYEEGSFIDTKKLSKRTKSDIDDTKLCFIDRFIAISPKKSIFIVSIKKKDDEFLSNIERILLSNTRKSDAVPLTELFAKTRIRTPDKSKYFDTFHKCQTFLHSGDSYELCLTYNTIVQIPDSIKPWEVYKSLVKKNPSPYSAFINFESSMLLSTSPERFISWTPKSCQMRPIKGTLKKHPKLNYEKACQLLRIPKEIGENLMIVDLIRDNLLYLLEKINVTKLMSVEEYETIFQLVSVIEGEFTNQFKGIDVLRNSLPPGSMTGAPKKRSVEILQQLEEQQRRGLYSGICGYWSVNDNADWSVIIRSLFRYFDDLECCEGFDTYRCGAGGALTVLSTDEGEWDEMLVKLHSVLQLFEETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQG0</accession>
    <name>A0A1E3NQG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ferroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_13917</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019434.1">
        <property type="nucleotide sequence ID" value="XM_019160131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQG0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQG0"/>
    <dbReference type="GeneID" id="30176818"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901684">
        <property type="term" value="P:arsenate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13851">
        <property type="entry name" value="CuRO_1_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13877">
        <property type="entry name" value="CuRO_2_Fet3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000024">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044130">
        <property type="entry name" value="CuRO_2_Fet3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133466" description="Ferroxidase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="42"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="11">
        <location>
            <begin position="156"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="361"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="606"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="621"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="634"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="651"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="74217" checksum="B10143A3FC4B565C" modified="2017-01-18" version="1">MKFASSLLSLASLSSLVLAETHHFQWNATYLTENPDGLAEVSDIVGCNGAYPWPTINVTKGDRVVIDLYNGLGVANTSIHFHGLFQHHNNKMDGVPGLTQCEIPPGESFTYNFTVPDQVGTFWYHSHSKGQYMDGMRGAFIISDPENPYAEKYDEERVIQLSEWYHDNITTLTKSFLNVFNPTGAEPIPQTLLMNGYMNSTLDVEPDKTYLLRIINVGGFVSQYFSVEDHNLTIVAVDGVYVEPNETEVVYITVAQRYDVLLKTKKDTSKNYAMMQLTDMTMLDTPTIITANVTNNLQYDASNGWPEEIEVDIEEFFDDFWLTPVHDYETYDDYDHQITIDVVMDNLNNGINYAFFNNITYVAPKVPALGTVLSAPNDTVSLQQAIYGSNTHSFVLQKDEIVEIVLNNNDTGKHPFHLHGHVFQVVERGPNYKTASDPVNYNESAPYTPRDKPMVRDTLYANPQSYFVIRFKADNPGVWFFHCHIEWHLLQGLAIVLVEDPAGIRANETALPEDWKDVCNSCGGHVGNAANNSVDFFNLAGENVQVVNLPAGFTTKGIVALVFSCISGVLGCAAIGIYGMADIPNMEEKVIQDLDIDERKLLEEMDEQDNLEESNSSSGRYSDDGANGKRFSDDVNTTSKRYSDDTTPIQEYHELEDVDKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQK0</accession>
    <name>A0A1E3NQK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_13961</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019493.1">
        <property type="nucleotide sequence ID" value="XM_019160154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQK0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQK0"/>
    <dbReference type="GeneID" id="30176841"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF169">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="362" mass="41716" checksum="22577D1E88D8EF6F" modified="2017-01-18" version="1">MSGINTTESSLLDALIQWKFFPDVNTFGIPDFSKPVPAWPFDFSNALLRTVVYPVSLNGYIALIFATTYFLSVHHFNKVVQNRQIEQFQKTNPGEKLPKNLKQLKAAPFKIATWRIFKVLVFLHNVFLCVYSVWTFIGMTRTINQISHKVFAVLFQDVKSSPLSRTELFWHCVCNNNNTTDNIWSNSQDLNVKGLTFYAYLFYLSKYYEILDTAIILLKGRPSSLLQSYHHSGAILCMWSGVRYMSSPIWIFVVFNSFIHSLMYFYFSLSCIKIRLPKWFKQSLTSLQILQFVIGGSLAVVHLFVRYYDSVNGSFSSCIATSEQALAVYINVFYLAPLTLLFAAFYIDSYKKKKAIVSKKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQL4</accession>
    <name>A0A1E3NQL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_32349</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019111.1">
        <property type="nucleotide sequence ID" value="XM_019162760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQL4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQL4"/>
    <dbReference type="GeneID" id="30179447"/>
    <dbReference type="OrthoDB" id="446113at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12611">
        <property type="entry name" value="RRM1_NGR1_NAM8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="63"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="170"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="300"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="41394" checksum="563D033B7B5BDE7B" modified="2017-01-18" version="1" fragment="single">MSSEDITNQTTSQDSVIPPSSSNATSLNRHDDSSSNSLPSDFLTSSVPLPQPPENPANTVPSKTLWMGDLEAWWDEEHIGNLWAKLDKHVEVKVIKPKHNLLMHQLAKTNGQGIVNHSGYCFIEFDSPDLATDALSLNGTTIPDSNNKTFRLNWASAATLDSQVAQTPEYSLFVGDLSAGTTEAHLLALFQSHFNSIKTVRVMTDPSSGLSRCFGFVRFSNEEDRRNALVEMNGKWLGGRQIRVALATPKHQNMKQSMYVNTINGPMPAASIGFYPHFNPNNRGNINSNMNQGFNDLNNTTVFVGGLANGLNEDTLLALFEPFGPIANVKVPPGKGCGFVKFAKREDAENAIESMQGFVIGGSRVRLSWGRPSNKNFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQR5</accession>
    <name>A0A1E3NQR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Topoisomerase 1-associated factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_13974</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019505.1">
        <property type="nucleotide sequence ID" value="XM_019160160.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NQR5"/>
    <dbReference type="GeneID" id="30176847"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="11">
        <location>
            <begin position="111"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="999"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1058"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1189"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1006"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1079"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1226"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1249"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1286"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1311"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1358"/>
            <end position="1372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1403" mass="160115" checksum="22E0FC8D09F74D78" modified="2017-01-18" version="1">MNEEGEFYGQEEEGFEELVEKSLGTALDDDQDDGEIEEDLLSDIEENDDGEDAEYSATVKREERLQSVMNMARNPLAVLSTGDQKDEQAIKRKVAMLCSALGGFDPNPPNEYILGVEALGCLKDIKELLKRIDEERNIWSVSSACHDSGLFVNDLIPILVQYGSFDTTNQNPETVKILLVGIELMVRLLTPLRLDDDSKENRIELHAKLKRAQIEYKYHLLHYKKGKIFKYLIALIIPILQLDLKNVTRRDNIILNLCLTLFANVLRIQPSDARSAKKGRNNVINVFETLPAGITEEDISIDVVLAIYKKYQVLSVVQTIASNLSKEFDTEILGTACADFYFHLFWNIDPTTITDETSIPTIQQSLSKKLNLSDANEHDDKTAVSAINSRLQQLINVENGRRRELFSKNSTRHANFGSLINIQDSKCNRVLSGQSHLLNNNVLEMLDSNASKTGSGKALNRKTKGQLGPERFIKLTYKTRSLLNKFVGDFIQNGFGVISKEIFSKLLKDASVQDYTFHYHYYFVVKWILKYEKSYQASNSKGVKVVERYKYLFYWFSKKAIDSLMNFILLLSRDKVYDVLTIGISVLKEIMQAAISIHSYEHFETSKLLKDDKNLLNAMVLRGEGTLRLIFSVNTEITELLNLPKNSHRKSHHLAMEMIEFTHTVLKVIKYIQCLKVPIMLVGKNPDSYDDDGLFEGDEVSKLDPKNLKRYKTLDKGQCDKFKELLYNDQTVDTHIWLFYQYKEIDESKLKMCLSYFSKLLECSESNILKLVRLDFMLVLYQLKDSSISTTLKVEFGDLMNFFMHKLAKMFSNTPTILLEPMSFNEMNDIEIKKYYLTGDPFSSADFTVREQKSMSKFGADDIRFVDDSISSNEKIALLVSHLYFEDKTAVIDDFVNFLSQWYHELNKSMSDEVKVVGKLNSWRLEGTCLKESRTNPYFRLLCRYGNIVNGILIKRESSELIDFKQKIEISLNTPLESFELENKFIDPNIARKARMKMSRKSLGDGSDEDDNDDYEDSHHGYGNEGINDDEDDLAGSGSDEQDALGLMEARLSRLGDRVKGKAMRRNADGELVEMGNSGRRKTRKRRKRRLSSDSQTQEKVVKNKKRIYKRRKIVMDDDEVLSDREILRRTHLSKEFINNSDDELSDDDEFFAREQKLQKLLQKRQGRITKEQYDSLMDGTLNLQDVSDLDDHSLSDSENLGPQTAHSKTDREFLMKMLQPSEDRGDSDEDDEDKSDDSSDNNSQDFYSDNESDEDNAEEIIINNETSGHISADIVSGSSDEELVTSESEIASDTSITDSLNTGINVQDNATKDPLRSDDKGLTNSDKPTTGEVFVGRSKTQSSDDADSDIESNNFERQAKAVKLSDVREKSSTPTTQEDGDIFADLRAIHELMNTPNTAPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR06</accession>
    <name>A0A1E3NR06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_14060</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019612.1">
        <property type="nucleotide sequence ID" value="XM_019160202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR06"/>
    <dbReference type="STRING" id="763406.A0A1E3NR06"/>
    <dbReference type="GeneID" id="30176889"/>
    <dbReference type="OrthoDB" id="10251642at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140713">
        <property type="term" value="F:histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140719">
        <property type="term" value="P:constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007063">
        <property type="term" value="P:regulation of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000006">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="12">
        <location>
            <begin position="6"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="13">
        <location>
            <begin position="570"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="14">
        <location>
            <begin position="841"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="449"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="957"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="964"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1032" mass="118119" checksum="F72D219D1D0BB936" modified="2017-01-18" version="1">MSDIAIDPALFRRRLHLIQKNIFSNESFQKVNGLLIVVGSSDDSNPYQKSTVMHTWLLGYEFPATAIYITNKKIYFVTSVSKAKYLQPLVSSASSSNNIVIFSRNKDPEHNKNQFEKFFKEVENTISEGNNSVGVLSKDKYQGKFINEWNPLWDEHKAKFDLKDVSLGVSETLEFKDEEEQRNLRTAARTSVNMMSFFTDEMSKIVDEDLNVSNSKLVDKIENKIDDAKFFKSIETNKAMKKLGKDFDLNNLDWCYKPIVQSNGKYELKFSVESTNDKLSGPVIMASLGLRYSNYCSSLSRTFLIAPSKEMEKNYEFLFSLQEHLFATLKDGAKFSDVYGEAVQFIEQNRPELKNHFVKNIGSLIGIDFRDTAGVINAKNDRVFKENSVVNLVLGFSNLEDSEKGQYALLLSDTVRITGSEPIILTESPKLKKEIAFYFDENDNIKEEGELEASGSKIKSEKSTDSKSSKSSKSSSTKVKNEPSVKTENFGNTRVLKAKRRSEQNNAEEGQIQIHKEIQSELHSKRQKEGLERFNPEDAKDGSEQRAIFKRYESYVRESQIPNNVKDLKIHIDSKNQTIILPIMGRPVPFHINSYKNGSKIEEGDYTHLRLNFNSPGLMITKREELPYESGEDKQFIRSLTFRSKDGARMTEVLKRISEMKKSAVKRESEKRERADVVSQASLIEVNRPKRLDNVFVRPTPDTKRFPGFVTIHQNGIRYQSLTRDQRVDVLFSNIKHLFFQSCKGELLVIIHAHLRTPIMIGKKKTYDVQLYREATDMSIDETGNRKRKYRYGDEDELEQEQEERRRRAMLDKEFRAFAEAIADTSGGLVDLDIPFRELGFQGVPSRSAVVCLPTRDCLVQLVDLPFLVITLEEVEIAHLERVQFGLKNFDMVFVFKDFSRPVVHISTIPMETLEDVKAWLTDVDIPYSEGPVNLNWPTIMKTIQSDPFGFFQDGGWSFLGGDGDSEEEAEEETAESDFDPSDEDPQDEEDYSEDAEDEEEEEDDDDDDASVEEDDDDVADSDDLSEEAFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR24</accession>
    <name>A0A1E3NR24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_47648</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019634.1">
        <property type="nucleotide sequence ID" value="XM_019163133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR24"/>
    <dbReference type="STRING" id="763406.A0A1E3NR24"/>
    <dbReference type="GeneID" id="30179820"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000022">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="49"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="133"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="12">
        <location>
            <begin position="356"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="14">
        <location>
            <begin position="629"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="13">
        <location>
            <begin position="869"/>
            <end position="1220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1222" mass="138063" checksum="1C0CA7B074500581" modified="2017-01-18" version="1">MKMLTKFESKSSRAKGVAFHPRRPWLLVSLHSSTIQLWDYRMGTLIDRFEDHDGPVRCVAFHPTQPIFVSGGDDYTIKVWSTQTRKCMFTLTGHLDYVRTVFFHHELPWIISASDDQTIRIWNWQNRKEIACLTGHNHYVMCAQFHPKQDLIVSASLDQTVRVWDISGLVKKHSAPSGASSFGGHSSMNQFDQYNRNQPPQQDIFGNTDAVVKYVLEGHDKGVNWASFHPELPLIVSGGDDRAVKLWRMSETRAWEVDGCRGHTNNVPCVLFHPTEDLIISVGEDKTIRTWDLNSRSPVKQFKRENDRFWMIVAHPNINLFAACHDSGVMVFKLDRERPASTQNQLQELFYINNDNQVQKYNFSSKATSLPLLSLKKIALPWNKIKNISYNASENSMLIQSGDNDTGIYSYFNLPKEIVGALEPSVRGEGKANSAYFIARNRFVTFVKSSHKLNVHDLNNNVTKAITLDSDVKDIVPATPGCILLLKPTTVVLYDVQQKKEIGLLQMNNAKYAVWSNDGQYLALLSKHTIVIVNKKFETVMSMHETIRIKSAAWDDTGVLLYSTLNHIKYVLLNGDKGTIKTLENTMYITKVQNSNCFCLNRKGEVECLEIDPTEYRFKKALINKKFNEVLRIIKNSNLVGENIIAYLEKSGYPEVALQFVQDPETRFDLSIECHDLTVALSEAKKINTKPIWEKLGREALSQGNIEIVELVYQQLHLLDKLSFLYLATGDENKLSKMADIAEKREDIGSLFTNSIYLGDVERKIQTFLHTGLTPLAYATAKSNGLDDIAEKILADSGVSLKDISLPDQLLKSKPSGVLKPINKTTSKNWPLSEAKLSFFEQALSGNFEDLQLEDKAVNGVSTTNEKSDLIDNSFADDDYIDDNLGEEDAWDLGDEDLDVELDNAEEENENLTDTSKLPKELKSWVKNSKTPGAYIAAGKFDTAASMLNKQAGIKNFEPFRQRFLEIYTASKLNISTFDEESNGNLPVYISDVEKANDGKILGLVPGYDQLEELVQEGFKLFKSNQLESSIGIFRKIIYIIATLSLTSDEEESKCKEILNICKEYIIGLQIELARRALPPAEIKRNLELAAYFTHVKLQPAHRLNALQVAMTQSFKAKNYHYASYFATEFLKIMSSGNRAEQARKIQAKAENIATDAISIDFDPFTEWEICAGDFVPIYKGQEIVREKMLGVGYSKSWKGKICKITLVSEIGGIGSGLRLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR74</accession>
    <name>A0A1E3NR74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_15906</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the pacC/RIM101 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019176.1">
        <property type="nucleotide sequence ID" value="XM_019161016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR74"/>
    <dbReference type="STRING" id="763406.A0A1E3NR74"/>
    <dbReference type="GeneID" id="30177703"/>
    <dbReference type="OrthoDB" id="6155966at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050806">
        <property type="entry name" value="pacC/RIM101"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257:SF1">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC_RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="86"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="339"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="368"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="413"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="552" mass="61228" checksum="C9973CC82B344799" modified="2017-01-18" version="1">MVETPRSSKYSISSLVSADPQHTRGASSTPASASASASRATVTTTPLKKDSSSRSHIRDLLNPREPRPGNEASAEPPLASFPGSVSRASSGATDASCVGTTSSADTVSTTPNETPIRQPRSPQESRVKEEGHKHEHEHEHEHDDDDEEGQEEEEEEEEEEEEDSKGSEKLTCRWQGCSAEFDDAKPLYEHLCDFHVGRKCNRNLSLRCKWDDCQTTTVKRDHITSHLRVHIPLKPYVCKTCSKNFKRPQDLKKHTRTHINSTPKDHKRFYDDLTSKRSFEEMSSSTLSSPLSSSCEPSLKRPLFQHSITDSQLSSRYIYKQLQPNSNLYSGCLQYPPPPFKVQPQMGPGPYPQPALPQAAPLAAPPHHSSQGYYPFQFSSPPMHHQPYYHLPPRLQPQRIHSSPSPQPPPMALPQEQIQPQQQRFYSTPFPPYSKIQPHPLRHLNSSFTPPPSATPAGGGRAPPNVLERRFPGSPSVPLSISPAPAPPPLPLRHDQLHPSQSQTRYAQSYDNTVAKESCTSAPAGQTSNVGLAGPSTTTYNTNPVPLSYRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRE3</accession>
    <name>A0A1E3NRE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_28737</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CYC8/SSN6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019778.1">
        <property type="nucleotide sequence ID" value="XM_019162554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRE3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRE3"/>
    <dbReference type="GeneID" id="30179241"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000879">
        <property type="term" value="P:negative regulation of dipeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042304">
        <property type="term" value="P:regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000403">
        <property type="entry name" value="General transcriptional repressor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000078">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="126"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="163"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="272"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="306"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="11">
        <location>
            <begin position="710"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="408"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="451"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="453"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="518"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="563"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="609"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="628"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="863" mass="97991" checksum="4AF307C6F09ABA02" modified="2017-01-18" version="1" fragment="single">HPPATSTPSMSSYDKKFIQQCAETWIAIGTCADTIGQVEKAVEAFSTVLRYTPNNAKALTHLAHVYRTRDAYAEAADLYRRALALDQHNGETWGLLGHCYLMLDDLQSAYTAYQQALLNLQNLSVPKLWHGIGILYDRYGSLEYAEEAFVRVLEMDPNFDKANEVYFRLGIIYKLQGKLQKALECFQYILNRPPAPLTQTDVWFQIGTVLEQNRDFTGSKEAYERVLQSSPNHSKVLQQLGGLYSQPEAPFYDLDIALQLLNQSIELNNSDAHSWYYLGRVYMSKQDYPNAYESFQHAVNLDSRNPTFWCSIGVLYYKISQYKDALDAYTRAIRLNPYLSEVWYDLGTLYETCNNQIGDALDAYRRAASLDPDNPHIAERLMQLSKYEKEGGALPPPVQTQHQLTMPSHQMIQQQQQQQQQQQHQLNPANGLPLGQQFQLGNAPNLIHQQSNEQRAQAEAQRQAEVQRQAEAQRLAEQQQQQQLAEAQRKAEFDAEEKRKAEAEAEATKRAAGLQKQAEMKAEEEKKQAEKVKKEKAKKEKKDAEEKEKEAQRQRQRQLEINSENRKQAQQERELEMEKQRKFAETAEAEAKAKAEADAETAANAAADAKIKADEEAREQARIHAESEAQAQAQAQAQAEVEAEAENQAKVQAEAEAEANARQLLEAEKVINQEPLSNENARFINLEKLDYISPAGNKGVWEIARRTTRPKGSDVDAVIIIPTLKYANGDNKLVFVRQFRPPTGGVCVEFPAGLVDPEDTLEACALRELREETGYVGVIRKRSGVVWSDPGLSDANCEIVWVDVDMEQDENKTPLPHWMDNEVIEVITVRLDELEKSMEEWSNKGYLIDAKIQTFLIGLCMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS49</accession>
    <name>A0A1E3NS49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019989.1">
        <property type="nucleotide sequence ID" value="XM_019160358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS49"/>
    <dbReference type="GeneID" id="30177045"/>
    <dbReference type="OrthoDB" id="3997769at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12638" checksum="30E403D0345C0F5B" modified="2017-01-18" version="1">MMGSPLMIFRAFWHYVVMQIHLVYNLGLLYATFYSTPPLMAFVIGLTLFWMKVVYELYRDIMHRDDPLYSSNVPKEMSIAAAISASMGFVLNMKAQEGDLTDAWWRGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS51</accession>
    <name>A0A1E3NS51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70503</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020023.1">
        <property type="nucleotide sequence ID" value="XM_019163660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS51"/>
    <dbReference type="STRING" id="763406.A0A1E3NS51"/>
    <dbReference type="GeneID" id="30180347"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="6">
        <location>
            <begin position="244"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="591" mass="66833" checksum="A73188A6BBF9FAE0" modified="2017-01-18" version="1">MSVKSLYQVYNVAVGTSKVVKESLNVVTANCSVLQAIRGNDQLYTEAKIVSEKARASATNREYSQANVDSMAHHADQHGDREVDIFKLAKAKSSHARSQAKHDTHSSKHEQKHQHHQKRGFHTSVRRLDEEKKQPGKEEVIGSKVQPAVKLTQAAVPSSRLGRLFHYGTLAASVGAHVLQDSATHYIKHGKVPEMKSVLLSPRNIELMARKFSQMRGAALKVGQMLSFQDNSVLPPDIQQILLRVQNSAHYMPADQLEKTISFELGESWRQRLFASFDDIPIAAASIGQVHTAVTRKELDQVVVKIQYPGVADSIDSDLDNILTLLTSTKMLPPGMFLEKSIANARVELKWECDYIREASNTVRYKEMLSDEPAFAVPKVYHDLSDEHVLTMERMKGIEIVKGDWSQEVKNKISAEIMRLTLMEIYKFKFMQTDPNWANFLYNEKTGRVELLDFGACRDFDESFTKPYSNCLRAAVKQDRAMAKKYSIELGFLTGLESEAMTNAHVDSIMVLGEPFREATPSGLYDFEHQTITDRVRTNIRLMLNERLTPPPEETYSLHRKLSGAYLLCARMKAEIPSKKIFEDVIGLEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSC9</accession>
    <name>A0A1E3NSC9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_29169</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020073.1">
        <property type="nucleotide sequence ID" value="XM_019162576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSC9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSC9"/>
    <dbReference type="GeneID" id="30179263"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="573" mass="62941" checksum="38550D6ABC07E407" modified="2017-01-18" version="1">MSHHFNYTTSQRRASSTIVGSLGRSGLYVPQDFIKYGKKKIGSVLDSMEDDLHPAEEAEDGVEAEFEQEGGAFENESVLVADDSIESGGEDGDNDNDTEAIIETWDDAIKTGKISTTVSLELKTLARSSVPLVITFLLQNSLSVCSIFAVGHISSEALAGITLGAMTSNITAIATIAGLASSLDTFLPQAYGAKKYHLVGLIFQRCTVLIFTIMLFVCISWWIWAETLLTNFLPDKQSAIYAAQYLKVTSFGIPGYILFETGKRFLQSQGIFDASTYVLFFCAPFNAIMNYTLVWVLNFGYIGAPIAVSINYTLMALGLFVYTLNTKNEANPMRCWTKLESRRIFRNWGELIKLSIPNLIMIMSEFLSFEILTLLASYLGTVPLAAQSIIATMASLTYQIPYGVSIASSTRIANFLGAQLPKSAYTTCKATFVFTASIALLNFSFLFFGRNLIAQWFSNDLKVIKLAEDVFPLIALMQVFDALNTTSAGCLRGQGLQRIGGYVNLFSYYVVGLPLGAYLGFCWPNKENPSGLTGLWTGSTFALIIIGFVQSYFSLHADFDQLVIDAINRSNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSX1</accession>
    <name>A0A1E3NSX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_9625</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020257.1">
        <property type="nucleotide sequence ID" value="XM_019165114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSX1"/>
    <dbReference type="GeneID" id="30181801"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052574">
        <property type="entry name" value="CDIRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566:SF1">
        <property type="entry name" value="PROTEIN NUD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1108" mass="127134" checksum="04D17A84DA725E02" modified="2017-01-18" version="1">MSTKISERLEADSAQSVNYTKPADDDISTANTAQDLTANHGSQVSYAENTTEHENVNTDDCVVMDSATKRLEKNDNNNNINMYYVNPNFAPNRKADNEIEETDQVSDENSSIDRMKDDTSSMQQSWLPSILRGESWPEQNSNYISDVVNGITDYDLNDNNEKIPNPLLTVRETVLRKNDTNAKELADWRKYKEDNFFKMINNNKLKDLPSTQSTIPNTFANKFNKIHGINNADLLTPDSPLKLFDVRQNTYTKFMMHNMLNQLSPQKLENEHQNDTKVPKVIKQSEELYNKILTGSSSSGSLLNKAQGKLTDTTKDGPEREQDNHNLSNGNDSGVENPNNNVDVTSDAEEFSSFPSTTRKFVHDGEQLFENIREGLQQHKIPLTDIAAPYSQSDYTSADDDYEGNEIGDNSESAADGKTTYDDDDDDDDDDDDDDDDDGSSPKLALNQGSPYNIPNDQHKILSETEPYNAEFNVRPPQLRSQFRAVEDKATVYDSNGNNNAVKNKPKGLNFIPADEYKNKVYDKKLKKYVLKNEYDTNSSYTDDIEIQDGLDNISISDPDRTSNSILRNANYAREINHEETFDSTDTSKSSSNNTIDAETPFNESFAVSDGLLVEAISESYPAEDWNFVKEIDLSEFELKQLYHLDKMIPNIWYLNASHNEINQNFGIPKEIQVLNLAYNNFNSISAKFDMFQHLQVLDLSHNELTDLRCLKDLKSLTSLNLSNNKVESIEFLENFRMLHYLNLSHNKIGGKLDFRNYTLWFLEDLIIDDNETTELINLSELPQLVNLSANNNSITRILYSETEEEEYVETPHFNLRRISLNNNSLYEDIDLSEFPETKEIQLDRCFAENIRHISLYTEKISARYIPEEKSVEGFLRFSLQSNNLKSLYLTGGVLPRALPTFKDRFSSVNILDISAMDLEALPKKFSEFFPLLIDLNLNFNRLRNLNGLEKLRHLKQLKLLGNEIRNIEDITDYTSRVRMTLRLLDVRVNPLTTKFYPFIFYGEDDHSEQGGSAASGDSDANNFTSFRLQEREDIEAFSIEYSRLYEEPGMLKWAKKNRKHQRKLSREVREERNGYLVAMLVWFEHVKYLDGDVINELDRRAYVSGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT67</accession>
    <name>A0A1E3NT67_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_87877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019971.1">
        <property type="nucleotide sequence ID" value="XM_019165032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT67"/>
    <dbReference type="GeneID" id="30181719"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="156" mass="17312" checksum="D8DD4ECDABFEBD96" modified="2017-01-18" version="1">MASGEEGMSGWEWRLHYSSPGVEQRPRGKSAGPACVRWTRFPEIASSRHNGPVCSCHHGMSCRYTRCCRRHCNSLQVVVAESRAWHLATASGSSATEHRNPECTFCGVHCFLSVSVSHCGVHYTTTAAVAAESTLRTLRSLLCFRQRFSIGSWAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT77</accession>
    <name>A0A1E3NT77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019876.1">
        <property type="nucleotide sequence ID" value="XM_019163624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT77"/>
    <dbReference type="GeneID" id="30180311"/>
    <dbReference type="OrthoDB" id="3980126at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018865">
        <property type="entry name" value="STK19-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10494">
        <property type="entry name" value="Stk19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="375" mass="40636" checksum="ED4F1DD07FA18C21" modified="2017-01-18" version="1">MARSESLRERLVRLRSKTAKRGAGTGASSRASRDGGSELDSAPRKKSFFDDDEHTQATLVEAPENALPNMHFPGVDTGRPATRASVLTVARRLAGAYLFPQLGVGVGTGLLSQTSKTARISRQYPHVLVQDQLYAVFGGQQTLVDRLVCELVADGDLRVLDMNYENFGFTVLVPGDEFLRTTTANFRESEAPLRDRYVELLAADPAATQIAASDLLDADIGFSTLISRGLICISAAEAGGTGVYNITLPHLGGLLRVVKSSCKWIAALTAKTREHMVLASVLRERWFRPYVPPKTQTKKPSDADAHAAFKREYYTTDRVGTAQHKPDATGPGVNIVKFRGVGLDWVLALMVGAGLLESYESPVGLVYRSTGKALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTE1</accession>
    <name>A0A1E3NTE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_99519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020455.1">
        <property type="nucleotide sequence ID" value="XM_019165153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTE1"/>
    <dbReference type="GeneID" id="30181840"/>
    <dbReference type="OrthoDB" id="5223508at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23703">
        <property type="entry name" value="mS26_PET12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058940">
        <property type="entry name" value="mS26_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26163">
        <property type="entry name" value="mS26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="312" mass="35682" checksum="939D8585450A902B" modified="2017-01-18" version="1">MGKGAMKFGGKSGLLPKPKEIFKQPYKHQVYKKQTDSGYAEGILHPKNITRDYIPSKVFTPEQLLKRSAHEPAKKFTEDEIAKMPISQQFKVKNAQMRREYLKDSYQKEVERLENLEKHEELLKLEEEKLAQEAAKHTQSKAEFFTSPTLESYLSDPLVRPRTAEEQESLRIKKESNRLQTRLNADTQRAASLFELYNASINFAITEEKLEKLVDAAFDSKADEAWNNVASSTPNKISAVKNHVTFDNALVDIVLDNVNKGPGYEAVEDYLNGFTDDIKELAEQIKKEKNQQDIEAANENLNKVAEMKRTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTF4</accession>
    <name>A0A1E3NTF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_14767</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the 850 kDa m-AAA protease complex, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. Associates with the prohibitin complex, composed of PHB1 and PHB2, inhibiting the activity of the m-AAA protease complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020409.1">
        <property type="nucleotide sequence ID" value="XM_019160522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTF4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTF4"/>
    <dbReference type="GeneID" id="30177209"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF14">
        <property type="entry name" value="MITOCHONDRIAL RESPIRATORY CHAIN COMPLEXES ASSEMBLY PROTEIN YTA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="461"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="880"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ49296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="910" mass="102544" checksum="6934A94D896CBF2C" modified="2017-01-18" version="1">MLSSLRGFRPVLPGQACLKLVIQKAPSNKSVKSGLHSSRLFSSLNSSYTTQKRLSKIYTFYSKNSQKSRVQLGISNLNNKRFNSSGPHHPKYNSRGEREYTDEEAEEILKDLKHIWKDQTVSQNQKVIELFNKYEMGGLISPSASKIVSAILRDSEATVPGEKVENVQKFHQALMHYHMHKQMKHNNEEDPVFNPKKDETDAGSKKAEKEEDQKDAKLKDDKPFGFKIEVEKPKDGKQKPFEFRSRQSKDNGTKFLEMEIRLDTILLWLVAGGLLVYTFLAESFEKEITFQEFETSLLSKGYVDKLVVVNNETVQVILNDFGKNQPIHDSNVEYYFTIGSIDKFEEKLKRSQEAYNIPEEKRVPVMYIRSTNIWKSLWSILPTVLLFGFIFWSTKKLLSSSGGMSGGLFGNKKTFKKFNADENVKITFNDVAGCNEAKEEIMEFVNFLKHPQKYERLGAKIPRGAILNGPPGTGKTLLAKATAGEAGVPFYSVSGSEFVEMFVGVGASRVRELFKTARENAPSIVFVDEIDAIGKSRGKSKGMGGNDERENTLNQLLVEMDGFKSDDHVVVFAGTNRIDVLDPALLRPGRFDRKIYIGNPELDGRKDIFGVHLKEIKLDPKCDLEDLKGRLATLTPGFSGADIANVCNEAALIAARKNDQFVRQPHFEQAIERVIGGIERKTKLLSPEEKRVVAYHEAGHAICGWFLEFADPLLKVSIVPRGQGALGYAQYLPPDVYLYTTHKLLDRMTMTLGGRVSEELHFDSVTSGAADDFEKVTSMAQKMVLACGLSPKVGLVSYDMDRGNDFTKPFSDKTASVIDDEIHRIVSECHERCRKLLTEKSADVEKVAERLLAKEVITREDMIELLGKRPFPNRNDAFDKYLEGKLTEEPANEEKDDKKNEDGEGEAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTL4</accession>
    <name>A0A1E3NTL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:ubiquinone oxidoreductase-like 20kDa subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_14792</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020439.1">
        <property type="nucleotide sequence ID" value="XM_019160535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTL4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTL4"/>
    <dbReference type="GeneID" id="30177222"/>
    <dbReference type="OrthoDB" id="268400at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12280:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit B 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="4">
        <location>
            <begin position="86"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="212" mass="23727" checksum="9518D6735EE21A8E" modified="2017-01-18" version="1">MLQQYSSSLLRSSFRSSALLHNTSRAFSITPIRNGSLTPHTLPTDTPVLSERPLSNVMDYAITSLDKAKDWARKSSFWPATFGLACCAVEMMHASAPRYDQDRLGIIFRASPRQSDIIIVAGTVTNKMAPALRMVYDQMAYPRWVISMGSCANGGGYYHYSYAVVRGVDRIIPVDIYVPGCPPSCEALLYGILQLQKKMMSDTTGRMWYRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTN1</accession>
    <name>A0A1E3NTN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">37S ribosomal protein S35, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020614.1">
        <property type="nucleotide sequence ID" value="XM_019160609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTN1"/>
    <dbReference type="GeneID" id="30177296"/>
    <dbReference type="OrthoDB" id="10052321at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021036">
        <property type="entry name" value="Ribosomal_mS45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S35, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12298">
        <property type="entry name" value="Bot1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38337" checksum="8D0123084CCA88A8" modified="2017-01-18" version="1">MLGRTALENSTLSVKTAGLQVSQVRYRRTLAYPFYSKAGNIPPTRKGRKQTVFKRLMDQFLGPKNYKGDYYLNKYAYPKTNHTTNYIDPRNERGNALLEPLSEFDVSSDDVAESGRGRNRHTLQPFPLNSNCFTNLQVSNETKVQIVNDILLNKVPSQQVALKYGLKIQRIEAILKLREVEEDWEQKGLIKGDLKRLSNTMYKMFPLYNPEKNAENLTEIPIPRETMQSRFLTIAESEPFGPVDAAKEFNLEPAAVTLERLSEGGEHSSHQDAAKMKKRDGSFIATMHEGDKYAFKFTPVKVGKVGYRYGKVFRDNRKDRKIAYDAAGNKYYPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTT7</accession>
    <name>A0A1E3NTT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70952</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020519.1">
        <property type="nucleotide sequence ID" value="XM_019163782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTT7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTT7"/>
    <dbReference type="GeneID" id="30180469"/>
    <dbReference type="OrthoDB" id="196140at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053229">
        <property type="entry name" value="NADH-Q_oxidrdct_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024549">
        <property type="entry name" value="NADH-UbQ_OxRdtase_su21_C_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019721">
        <property type="entry name" value="NADH-UbQ_OxRdtase_su21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 21KDA SUBUNIT N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10785">
        <property type="entry name" value="NADH-u_ox-rdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12853">
        <property type="entry name" value="NADH_u_ox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 21kDa subunit C-terminal fungi" evidence="3">
        <location>
            <begin position="98"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20891" checksum="5352BFD98966BC3D" modified="2017-01-18" version="1">MAKYEVIDQDPVFSKVVSYFRVSDYLTWLGATAAGPLSLILLEKYEPASGRHFKMPKPMFLRAGGLVGFATGFFLAYNQSTKRFWGVSENAREVEKDRYEVKSLLAKGKNPYGSDESTLTPYMQDLSARYSKNSQLLIAILPWFNFVAHPYHGVDLKKYYEVREGEEKWGFNLVPLNEIKGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTU9</accession>
    <name>A0A1E3NTU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fructose-1,6-bisphosphatase</fullName>
            <ecNumber evidence="5">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-fructose-1,6-bisphosphate 1-phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_71012</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="5"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020582.1">
        <property type="nucleotide sequence ID" value="XM_019163802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTU9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTU9"/>
    <dbReference type="GeneID" id="30180489"/>
    <dbReference type="OrthoDB" id="10256725at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000009">
        <property type="entry name" value="Fructose-1,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006778">
        <property type="entry name" value="PRK09293.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="14">
        <location>
            <begin position="13"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="15">
        <location>
            <begin position="210"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ49469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="341" mass="37396" checksum="F949A3474FA4E035" modified="2017-01-18" version="1">MSDSKTPIDTDLITLTRFIIEEQQRLAPEATGELSLLLNALQYAFKFIAHTIRRAELVNLIGLYGQTNSTGDDQKKLDVIGDEIFINAMKGSGNVKVLVSEEQEDLIIFEGNSGNYAVCCDPIDGSSNLDAGVSVGTIFGVYKLQPGSTGSIKDVLRSGNEMVAAGYTMYGASAHLMLTTGSGLNGFTLDTNLGEFILTHPHLKLDDSSHKKIYSVNEGNSHYWSDDIKEYIASLKQPQADNHDKPYSARYIGSMVADVHRTLLYGGIFGYPADSKSKTGKLRVLYECFPMALLLEQAGGMAVNDKGERILDLTPKKIHERSGIWLGSKDEVEKLLTFIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU12</accession>
    <name>A0A1E3NU12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="1">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_28836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="1"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020642.1">
        <property type="nucleotide sequence ID" value="XM_019162558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU12"/>
    <dbReference type="STRING" id="763406.A0A1E3NU12"/>
    <dbReference type="GeneID" id="30179245"/>
    <dbReference type="OrthoDB" id="14419at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054491">
        <property type="entry name" value="MGH1-like_GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF10">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 63 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22422">
        <property type="entry name" value="MGH1-like_GH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannosylglycerate hydrolase MGH1-like glycoside hydrolase" evidence="2">
        <location>
            <begin position="452"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Mannosylglycerate hydrolase MGH1-like glycoside hydrolase" evidence="2">
        <location>
            <begin position="729"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1029" mass="119958" checksum="A7AB5C4E98F25C65" modified="2017-01-18" version="1">MPLSDKPSKEEKRLAEDKKRTKYWKKWGTYLSERCWGVPREDYTDEGDAWQFDFETAKSRVFRWNEQGMAGVCDTHQLINLTMAMWNENDGILKETLFGLPGGASGKSGVHGEDVKEMYYYLDNTPTHSYAKFLYKYPISKFPYEELYEENSKRNKHDTEYEITDTGVFKDNNYFDVFFEMAKDDEDPEELYFRITAYNRSNAAAPLHLIPQLFFRNTWAWNLDSEKDREKPTMYQEHDNVIRINHYKLGERRIVFAPAPGYEGSENDVEPKLLFCDNETNFQKLYNVENKSEYCKDGFHDYIVDEIKTSVNPEQNGTKACAWFVFDENGGIPPGDYVTIRYKLTKDLSDESLDLDEELLDDIISARINEADEFYWNVSPLPIPQHLRNVQRQAYSGLLWTKQFYHFIHDYWYRGDPDSDLPPTVGRANERNKDWKNLYNENILSMPDNFEYPFYCSWDTAFHTIPLAMIDPEFAKSQLDLLTREWYMSPHGQIPAYEWNFSDTNPPVQAWAAYRTYKIEKKNWGTADRPFLERVFQKLMINFTWWVNRKDVDGNGIFEGGFLGLDNISIFNRSEPPSGIQLLQSDASGWIAWYALTMMNIALELARDNPVYEDIASKFFEHYLLIADAMTFLNKSEKNFRPTDDSLWDDEEQFFYDKIQWGDDKKSSVKVRSLVGLIPLFATTTLEAEVLDRFPSFKKRLKWLIQNKNYLFKRHIASMETKGDGDRLLLSLVNKDRLIAILQKLFDESEFLSDYGIRSLSKYHEEHPVHMNINGQDYGVSYLPGESDSGMFGGNSNWRGPIWFPMNFLLIESLLRFYLYYGSSLKVEVPVGSGEYLNLGQAAEEIQQRLIHIFMPDKDGFQDQQEYNETLKTLNKDENFKDLVNFYEYFDGDTGRGLGAKWQCGWTALVARMIQDVGVVCRQPHTPIRSKSFYDQDQIQNYEPGTGAYPLLGRRKSGKSLLNLAAQRLELDTDEAVAQLPGLSRRSSTKTNASDQSANEDFLRLVKEAMANYKISDEVNVNSEEYEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NCZ7</accession>
    <name>A0A1E3NCZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phytanoyl-CoA dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_18525</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PhyH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015164.1">
        <property type="nucleotide sequence ID" value="XM_019162042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NCZ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NCZ7"/>
    <dbReference type="GeneID" id="30178729"/>
    <dbReference type="OrthoDB" id="445007at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883:SF45">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="40448" checksum="7120B9A5A4465376" modified="2017-01-18" version="1">MTFPYTNCTTTTLNRLQITPLETSHEVSETPSLAETKIAKVHLPTAIPALDVSPTAFTLTKEEATPDAVMELLRKYGICTIKKLFDASEIDSINEELDPLFELKKNDPRLFPKETIRVTNTVSKSPIVVNKILSHPLNLAVAHKALDQANAFWIGNNINIGTCPAIVSSSIAFQISPNAACQALHRDDQSDHNIRYPQTYESFSFNSESQIGFSVALTKTTRQNGATRIIPGSHLWDHLQKPNEEDCIYNEMEKGDATFMLASVLHSASSNLTKDEVRRILILFMGRGIYRQKENIFFNADLEYFKQFSVEQLRRLGFAMSEPYGNMVELQDPLVKIKEGYRRTTNYSDICRVNSQYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDA7</accession>
    <name>A0A1E3NDA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_37688</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="2"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015218.1">
        <property type="nucleotide sequence ID" value="XM_019163098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDA7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDA7"/>
    <dbReference type="GeneID" id="30179785"/>
    <dbReference type="OrthoDB" id="10264412at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="95"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="199"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="807"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1060" mass="119845" checksum="466E79B56B5EFB76" modified="2017-01-18" version="1">MLRLARNIHRAQPRLLPLALQKNVPRAGFSVSLNRNNESAATDKETGLHKYADTVILPKTEFGSRSDQKLIETVLKPQTMQKLYEWNISRPLKDNRIENLFIFHDGPPYANGNLHIGHALNKVLKDIINRYELMLGKKVHYVPGWDCHGLPIELKTLEKLGKERKEVEKKLKKDLKKASSNEEKDQIRARLHSLKHTRLSPTDIVRLSSEHAMNTQKSQSKEFQEIGIMGDFEHPYMTLKKRFVTDQLRVFKKFFDNGLVRRQEKPVYWGCENATALAEGELEYNQQHQSKAAYVKFPIVEVSEALQKSLGSEIIEAGISALIWTSTPWTLASNLAISINEGFEYTVIHNEKFGNLVVSTELIPSLEEIFEFSKSEVVFKGSELLGCKYESPILNNGHKYPFLHGSHVTSTAGTGLVHTAPGHGQDDYLVCLQNGIKPYSPVDKYGKYTDALGENLAELVGFRVLGEGNAKMIERIKELGILIHLNENYIHSYPYDWRSKKPIIIRATPQWFIDVSKIKDVTIEALKKKVNFHPERGVNRLTSFIKTRNEWCISRQRSWGVPIPVLYHRETGEVLLDDAVIGRIIEVIELEGIEGWFEQVEKSQQGADIGKWLPDEYQHLAKDYVLGTDTMDVWFDSGSSWKTIEHYLEDEGLLEQAKARGYLSDVYLEGSDQHRGWFQSSVLAKVGSEINGNGDVVMPYKNIITHGFTLDEKGEKMSKSLGNTMSPGDILQGNKQLGVPKIGIDGLRLWVAQSDYTNDINVGPTILKHVGDNLKKLRFTFKFLLGNLNGFDTAKSSLRYEDMNRLDQYVLSRLARLNAECLHEYEAHNFFKIVKLVNHFINVDLSSIYFDVRKDALYTDRADSPKRVSTLVAFNEVLHTLVSILAPIVPIITQEVWNSLPDGIKGALDSPFHRGWYNSAYVRVNEDVENDFAHLLDLKDAVNGMIGQLRAAKHIRSPLECRVVLQPAVPTPGDDSIAVDAVLPRYSDPELEDLFVVSSVVRNEPNPVADSRFHVQESVRGVHLHVVEAPGGKCPRCWKHAPVSAGLCLRCADAVGVSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDC6</accession>
    <name>A0A1E3NDC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_13883</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015244.1">
        <property type="nucleotide sequence ID" value="XM_019160120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDC6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDC6"/>
    <dbReference type="GeneID" id="30176807"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133314" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="8">
        <location>
            <begin position="28"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="9"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="497"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="541" mass="59531" checksum="998F714E93BF62DA" modified="2017-01-18" version="1">MEKRNIFVVYFFILMATCLDNLNSATAFTLQGNIQQAFNTDSSTASWVLSGYALTLGSFIMVSGKISDIIGPHNLYLGGLSVIWICALICACIPHSSIITLIVFRAIQGVGASALVPSTLALAANYFSGPYAKYLPGAVVGFILALTGIFGVALVLGGAFSETAIGYKSYFYFVFAYGLVMNIILLFLIIPIEETEEHKKMKLKNVDFIGSALTIIGILLMILGLTEGGDDWKRPRAYVPLVVGFFTFVSSFIFEMFYLKRFQEKNQHRDKSEDWRLQIDLIFPPEIIHIPNFLPILIACGMYFATFTMITAIGVLYYSSIDGDSPIIVALKVAAISAGVIFGATTYRQSYYGKIGVRNMFIISAAVSLGTCIWFSRTNFHSHKSYWKFGFVSLFLYGYALNVFFNIYIGVVVSNTPLHLQGVVNGVYQTCSQVCLSVGNALVPSIVGNVVPATSEEMKQHLHNKFQNIFYVLMGFHVVILIMMVLFVRAPKKSEESETAEATAEEKPVDVEMGCDGTCSEKEEEGSCTNISLHGDISTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDC8</accession>
    <name>A0A1E3NDC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18577</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015235.1">
        <property type="nucleotide sequence ID" value="XM_019162067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDC8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDC8"/>
    <dbReference type="GeneID" id="30178754"/>
    <dbReference type="OrthoDB" id="427186at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000006">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="9">
        <location>
            <begin position="9"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="7">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="234" mass="26137" checksum="59E5095E41E1FADD" modified="2017-01-18" version="1">MSETGEVRAVVPLESNPEVFTDFAHKLGLSPLLEFTDIFSLDDPDMLAFLPRPVQAIILLFPVTQAYEEFKNAESVEELSSDKVIWMKQIVKNACGLYALLHVLLNIQDGLIVQQSAVANFRRNLLHGNANPVTLVQNIAKELYTNYSQQGQTEAPPAEENVELHFVCFVKRPDGIYELDGRRNGPILLKKTTDENGDILSDDCINHRVKKYMSLVSGENALNFAMMGLAPSMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDD7</accession>
    <name>A0A1E3NDD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_79879</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015245.1">
        <property type="nucleotide sequence ID" value="XM_019164974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDD7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDD7"/>
    <dbReference type="GeneID" id="30181661"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="50806" checksum="3FCB38BE46E60AD2" modified="2017-01-18" version="1">MGKERHPNGKEKPICGLLFHPYGNLLDNLNNATAFTLQGNIQQAFNTDSSTASWVLSEYALTLGSFIMVNGKISDIIGPHNIYLGGLSVIWICALICACIPHSSIITLIVFRAIQGVGASALVPSTLALAANYFSGPYAKYLPGAVVGFIIALTGVFGVALILGGAFSETAIGYESYFYFVFAYRLVMNIILLFLIIPIEETEEHEKMKLKNVDFIGSALIIIGILLMILGLTEGGDDWKRPRAYVPLVVGFFTDKSEDWRLQIDLIFPPEIIHIPNFLPILITCGMYFATFTMITAIGVLYYSSIDGDSPIIVALKVSTISAGVVFEASIYRQSYYGKIGGVVNGVYRTCLQVCLSVGNALVPSIVGNVVPATSEEMKQHLHNKFQNIFYVLMGFHVVILIIMILFVRDSEKSPETEDEVEDKPEVGGNCNKVEKGYDGICSEKEEDDKCTGIFLHGDSLEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDE7</accession>
    <name>A0A1E3NDE7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_56587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015255.1">
        <property type="nucleotide sequence ID" value="XM_019163189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDE7"/>
    <dbReference type="GeneID" id="30179876"/>
    <dbReference type="OrthoDB" id="1060854at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF94">
        <property type="entry name" value="PHO85 CYCLIN-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="228" mass="25864" checksum="266ED62CEE201B5B" modified="2017-01-18" version="1">MLSALLQKIVEANDLLHTEEDHLNRSHTSSNLGTHNNSSKNNNTNTSNTTFDSTSGGGLLNSKYGANVLAFHGRNIPAISLHAYLTRILKYCPVTNDVFLSLLVYFDRIAKQNNDEGPQVFVMDSYNIHRLIISGITVASKFFSDVFYKNSRYAKVGGLPLDELNHLELQFLLLLDFQLMIQKEELERYGDLLLRFWKREQIKRMSTNGMESQPLQTATDSDNAMTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDN2</accession>
    <name>A0A1E3NDN2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015350.1">
        <property type="nucleotide sequence ID" value="XM_019164891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDN2"/>
    <dbReference type="GeneID" id="30181578"/>
    <dbReference type="OrthoDB" id="10438186at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="115" mass="13397" checksum="3D86BEA0BA71CE10" modified="2017-01-18" version="1">MPYCDMILLIKTTNMLIYSNTHDPLDNNQACATQHTMTKVYIQHETLPVSYKSKPALKRWSDTLIGMFYYTTPSFVMTRFENCVCSVIGLVLVMLFVYGCKNYVLPLFYLLKQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDW3</accession>
    <name>A0A1E3NDW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015425.1">
        <property type="nucleotide sequence ID" value="XM_019160073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDW3"/>
    <dbReference type="GeneID" id="30176760"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="121" mass="13188" checksum="4365E11893ABDD38" modified="2017-01-18" version="1">MASLPLLSPFLLHHLRHRTPYCYPRLPPHTPRPPRHCHDGSVNHLPRLSGEGPPELRRGTGQRENGPQGSAECAHPRGLADMHTPTSMLPAAGCTATVLPQQPETRDLRIPDGPQVRASVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDY5</accession>
    <name>A0A1E3NDY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015454.1">
        <property type="nucleotide sequence ID" value="XM_019164811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDY5"/>
    <dbReference type="GeneID" id="30181498"/>
    <dbReference type="OrthoDB" id="2564148at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="7"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="584" mass="66277" checksum="76F3AB1FDEB45FD1" modified="2017-01-18" version="1">MFFSEPATSLMDFPDNVVDQILGNLGARSLLSLKQTCRFLADACDRHLYRNVYFYDENRQMNALRSETQLPLKYTFIPVSRMGWFVSLMDRNARIPQTMASIVMHTSLNEQTKKLLALLFDSSGGRGGVREVLFKGHDVRFLSYLSDYSSNVTASVGSRLYENDEVYDRAALLDGASRPPTANTNMGAAEAATVGPQAEFVPSKKVELVSTQIHSPLDADRVLQLKLALSGVEIRFPNAYPESGLSRSLIRYWSSSLKSLTLANYASFLYFANGLASYSRSLDSYRGELFPNLETLSICFTDDISNNESMGTALQLLNLANVKNLEIKYKRSIFYDSTATNLELVQYLNRNISFSALKQFSLVNLNSNNMLANNIHEHEISENTNLCYAIMDHLDLFSTNTETLTFLNLCLNTFPVTPMVSRSPDTGDPKIFYANSEYIARKKELFEKIFLLRSLEILIIPDFLFNWWPFLEENQNMNFHQDKGSEPNMIPILYRRFYSFESGSALNIMDFPPNRYGSNFGRRLNSYLDEIVPSIAHFAQLLPRLAFLNLGGVLLEIHRDARGDVEKLSGVYDSWVFTDFPIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDZ2</accession>
    <name>A0A1E3NDZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_20267</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015452.1">
        <property type="nucleotide sequence ID" value="XM_019162156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDZ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDZ2"/>
    <dbReference type="GeneID" id="30178843"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17781">
        <property type="entry name" value="CBS_pair_MUG70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17782">
        <property type="entry name" value="CBS_pair_MUG70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="522"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="73"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="184"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="250"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="5">
        <location>
            <begin position="400"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="61162" checksum="CA4303656CFCEFA0" modified="2017-01-18" version="1" fragment="single">GTVMSLNPSAPLICASNVSIYEAAQRMIAAKENHVLVVDENEELLGILTTKDIAFRVVGSNLNIKSTTVKKIMTSNPMCAKVATKASDALSLMVIKHFRHLPVVNEDNQIVGVLDITKCYNEAMKRLEKLYQDSKKLYDAMETVNKELGNSGYGRRQHTYIITYFENLKRILSGPTLSQLLNDESTLPVYCSVDSSAYDAAMLMKLKKTTAILVRDLKEETVGIFTSKDVVSRVIAKGMDPTDCTVESVMTVHPATAPTTMSINEALKQMFEGKYLNLPVLDEKTKDIVGVVDVIRLTNFTLNQIQTMESFNEDEEDADDEELFDSIGENDFSKFLQTLPNENEEYGYDSSASDISVDELAQFDLSTMSNDTNTVCSRNIEGPKRMSSIMSLGPINYNDVCFFKFKVSRGRIHRVSYRPSDGIDKFKELLKEEFMKEELDMFDQGIFEISYLDEDGDIIVINTDKDLKDCVLLMKSLQKDKIEIMLYDKHEVLVGKKGKMSTLNLRSSYNKKRGTENTGKDYMLPAAIFTLAATIVVVFTLSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE69</accession>
    <name>A0A1E3NE69_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015543.1">
        <property type="nucleotide sequence ID" value="XM_019164838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE69"/>
    <dbReference type="STRING" id="763406.A0A1E3NE69"/>
    <dbReference type="GeneID" id="30181525"/>
    <dbReference type="OrthoDB" id="3994636at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="382" mass="43081" checksum="7AFB7B333046FD13" modified="2017-01-18" version="1">MCLRCGKEYTTQETHCYRCYTCPICDEDLESHPLLYKHKAGSTEVEFELIKSKHDKSKIAAKAVIFKCPNSACRFKFATKVETKPQSLQQIVQDNIKDDTDIRYNALLNYYDWCLNYQRILDKRQRSKWKTEILGRFKSFEIAKILQNDSNSQQLIDKESGNIRSIHDEEGNQICPHPKLLHTEMLEICPTCLEDISGNRMAFKLPIVYAIPMVGYPSSNVKELQTGETVDVPILLSFVNNGEVSINLGINEDGVDVFLASSLAHNVCIPGINEFTKQKSGELSTVPTCLLSHISKKEGNWKTELAGRDPGALTRLRYVEALSFDGSEDTIDAGVNWITSLILVRVTSSERSRKGKINFVLNVSVEQGENVSNAEYHCCALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE95</accession>
    <name>A0A1E3NE95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_19344</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015580.1">
        <property type="nucleotide sequence ID" value="XM_019162115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE95"/>
    <dbReference type="STRING" id="763406.A0A1E3NE95"/>
    <dbReference type="GeneID" id="30178802"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="7">
        <location>
            <begin position="34"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="6">
        <location>
            <begin position="202"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="41461" checksum="85474DA7B15F8215" modified="2017-01-18" version="1" fragment="single">AVPEKFTGFAVHDPSEATWSTFKSYSYDPKPFEETDVDIRISTCSICGSDAHSARNGWNKPNAVYPLVPGHEIVGEVVRVGGGVKDFKVGDRVGVGAQSQACLKCKMCLSNNEVYCPQLVHTFGGVYHNADGTTTMSQGGYANYVRIHEYFVFHIPEELSDAEAAPFLCAGVTTYSPIIRNLPHDLIARGVRPKVGVVGLGGLGMMAIQFAHALGCETVVFSRGESKRGDALKLGADRVVITGTAPEGKSWLEDVEENRDVKFDLDLVICTANSADNFDLNTYLKVLKINGKWVSVGLPETPFTVSPRSFMSNACFMGASHLGSHVEMVEMFKLAVEKQIRPWVECLPMSAENCKVGIEKCHYNKCKYRVALTDVEKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEN9</accession>
    <name>A0A1E3NEN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase RAD53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_18003</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015702.1">
        <property type="nucleotide sequence ID" value="XM_019161844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NEN9"/>
    <dbReference type="GeneID" id="30178531"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009202">
        <property type="term" value="P:deoxyribonucleoside triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043247">
        <property type="term" value="P:telomere maintenance in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016256">
        <property type="entry name" value="Ser/Thr_kinase_Rad53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000661">
        <property type="entry name" value="Ser/Thr_PK_RAD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="9">
        <location>
            <begin position="134"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="262"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="9">
        <location>
            <begin position="714"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="551"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="635"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="565"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="640"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="664"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="900" mass="101658" checksum="37ECECEE57E581E0" modified="2017-01-18" version="1">MAERPGYRTNIISAPLKEIRNSVFGQESFIQNQEEFMNKVKPPNQPNNETETKKLPDTTTQPTQPSSYNPLTDPLGHELVRQGVLCRLIGRTFDETFHIDVKSKDGNKIRVSTDNNNTSNHNNNEVISNLKQEWIFGRNSHTCTYLLPVQSTRISNKHFKLWMNIDNDNNQNNGTGSSRQENNLMIQDLSTNGTWLNNSKLVQGRNYILTQGDEIAIGIGIEKDVARFVVHLPNVMLTKMMDENGSLKALSLEDKGVHKDFIIRDEIVGSGAFATVKKAIERSTGITFAAKIISKKKALGGMDGVSRELQILKKLDHPGIVRLKAFYEDDDNYYLVMEYISGGDLMDFVACNGAIDESASREIARQIMEAILYVHSKGISHRDLKPDNIMIAQDDPVVVKITDFGLAKSQEQESRMKTFCGTLAYLAPEVISNKKKQSNNRKRYLGNGRITEDLYSNKVDMWSIGCLLFVIMTAHLPFSGSTQDILFKHITNGDYHDKLLRSMDISIEGCDFISRLLEVDVALRLNAAQALQHPWFNETIDFPSQVSLSQHLSQSQRNVGKQIPKKLEQNDTDNKKSNNTRGKFMKDFKIPQIPKDRNMHEDSMNFMGASTSQIENSHALSSLTERQDMDYKNFEDEASSKPNTPQQKGTSSPSSQSMLLQARSKSQSQSHSQSKIKSQPKPPPATFMSLSIIQPGSLKTKLSTIYIPQGTPAFYIGRLDNLNVQMNDERISKIHCMIVKRRHPTKDTSSGTSSNSIVNTPYNLENNGFDENGIYSSPAMGLDDVWLLDFSTNTCYVNGMKIGKGKKIKLRDCDVLSLFVDKKHDSELKYLIRIVDTTGLYIDDVTSNLERKRVTIDESDRKLFETKLKEGLKQVEVHVMQRKRKPVESIAEHPNKRVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEZ0</accession>
    <name>A0A1E3NEZ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_174776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015769.1">
        <property type="nucleotide sequence ID" value="XM_019161618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEZ0"/>
    <dbReference type="GeneID" id="30178305"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="155" mass="16666" checksum="8E0CF153B87D3A91" modified="2017-01-18" version="1">MCHGGAEEQCKAGRRGLTRGGAGKGGKETKGRRGKHGRGNRGRREEGKRPPGTETHRQKAGKRKETTQPAALLRARAETTRGSQPAGIPASLPAFPDHHRSSLSRGEEHPAGPPVCCRKWAENHNNNKSLDDARSNRSNNTMPDAAVTKDNVALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF00</accession>
    <name>A0A1E3NF00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1445 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-glutamate cyclase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015815.1">
        <property type="nucleotide sequence ID" value="XM_019161879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF00"/>
    <dbReference type="STRING" id="763406.A0A1E3NF00"/>
    <dbReference type="GeneID" id="30178566"/>
    <dbReference type="OrthoDB" id="10262538at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047820">
        <property type="term" value="F:D-glutamate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2040.10:FF:000001">
        <property type="entry name" value="D-glutamate cyclase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1640.10">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2040.10">
        <property type="entry name" value="PSTPO5379-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009906">
        <property type="entry name" value="D-Glu_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038021">
        <property type="entry name" value="Putative_hydro-lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022:SF10">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07286">
        <property type="entry name" value="D-Glu_cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160920">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30570" checksum="BC4D7B5546149E44" modified="2017-01-18" version="1">MTVDISPSEFRRLCKENSFWGHTSGCVPGYAQANVLVLPKEIANDFADLCARNPVACPLIAMTKLGSPNKVDNRLVIDDEDFDLRTDLPKYGVYKQGNLIREASSVADCWDLDSHIGFLIGCSFSFEAALISEGLAPRNVLEKSNVSMYLTTKMLNPAGVFTRCPYVVSMRPYKATDVEKVRLVTREFRKTHGEPIDWGYDAVERLGIKDLLLPEFGSPCRIEDDEIPVFWACGVTTQVAAQQAGKHSANDDYIFAHVPGHMLVLDIKDSEICCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF19</accession>
    <name>A0A1E3NF19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">diphosphoinositol-polyphosphate diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_36868</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + H2O = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:79699"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:79700"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3,5-bis(diphospho)-1D-myo-inositol 1,2,4,6-tetrakisphosphate + H2O = 3-diphospho-1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:56312"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:140372"/>
            <dbReference type="ChEBI" id="CHEBI:140374"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:56313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:22385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>6-diphospho-1D-myo-inositol pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:79703"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:230534"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:79704"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Atypical dual-specificity phosphatase Siw14-like subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.52" evidence="1"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015835.1">
        <property type="nucleotide sequence ID" value="XM_019163041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF19"/>
    <dbReference type="STRING" id="763406.A0A1E3NF19"/>
    <dbReference type="GeneID" id="30179728"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052840">
        <property type="term" value="F:inositol diphosphate tetrakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14528">
        <property type="entry name" value="PFA-DSP_Siw14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000024">
        <property type="entry name" value="probable tyrosine-protein phosphatase At1g05000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF48">
        <property type="entry name" value="INOSITOL PHOSPHATASE SIW14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="166" mass="19540" checksum="F6D966441FCC035D" modified="2017-01-18" version="1">MYVPPENFARVCEGIYRSSFPRIENFEFLKSLGLRSILCLIPEEYPIENSKFNESNGIRFYQIGLSGNKEPFVKIKPELVTEAIKILISPENQPVLLHCNRGKHRTGCIVGCIRKLQKWSHSMIFDEYRKFAYPKERPLDQQFIEMYNGHEVEDFAVQNRLLPIQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFN3</accession>
    <name>A0A1E3NFN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73742</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016054.1">
        <property type="nucleotide sequence ID" value="XM_019164571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFN3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFN3"/>
    <dbReference type="GeneID" id="30181258"/>
    <dbReference type="OrthoDB" id="205108at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF10">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="507" mass="57922" checksum="12A2C73FAC67EAFA" modified="2017-01-18" version="1">MHQNSDLKARKKQQSSVLPLALKKPFQVSSSSVSSTARKYVPIVLLLFLLHFVLVGSILPAPLVSLISFVASPVTYFFSANNAPSSISMFTDSKYLGSKYQHGLTSNKKIIEEKNKYIFPLIQEAPFLKELGLDKLFKVTQDGSNRKFYSYSPDYFDDLSATNEMTNENAKDKTPEMQSMQTYADAIKHFKANGHRIYTGKEKPEVVLVTAINFEELEPSYLVKIIQNRVDYAHKNGYAIYSRWVQEFTPIFQEYKNDPEGWSKIFILREAMYAFPHAKWFWYIDEYSLIMRDDINLNNYLLRPKALDPIMLRNQPLIPPDGAVKTYTHTVAEDVSLILTQNERNLNTDNLIIKNDLTGRAILELWMDPLFRKYPSFRKDESNALAHLLQWHPVILSKTAIVPPRAIAALAPEKAPETRSNEFIYQSADFVVSLGDCKISKDCERILAPYWDKTQMLGANQGNGQEQPNHAAQEKLNKRDTTDKIDKIMGAEDNIANDADIVNNENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFP8</accession>
    <name>A0A1E3NFP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pheromone-regulated membrane protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_37201</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThrE exporter (TC 2.A.79) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015511.1">
        <property type="nucleotide sequence ID" value="XM_019163066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFP8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFP8"/>
    <dbReference type="GeneID" id="30179753"/>
    <dbReference type="OrthoDB" id="413008at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051361">
        <property type="entry name" value="ThrE/Ser_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082:SF4">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/serine exporter-like N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/Serine exporter ThrE" evidence="9">
        <location>
            <begin position="259"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="427" mass="46573" checksum="100DB5308D45DCF0" modified="2017-01-18" version="1">MLFGAPNHRLEEYMSLTAKVLEVEATFIYLPGMMLANFSDPVTRTSDLKLVRVGQGLNLNKLDQAHDIYESVVYDRIGVDQASKELADLFSSKEFLNTYWIILLYGLSSMFVLIFFNGAWLDMIPSFCMGALLGFLQCVVAPKNALYSSVFEVGSAILLSFIGRAIGSIANGRYFCFSAIVLSGLSMILPGYMILRGALEIQSKSIVSGVVGMFYAIIYSLFLGFGLTLGAGLYGWIDKNAITETSCSNIGHIGNVWKLLFVPLFNVCMSLSCQARFHQLSIMVVIACCGYVVTYFSSLHFSITQFNSALGSFAVGLLSNIYDRWLRSYRRIGYCSTKFTSMICGIFDLVPGGFAARNVLSSGLLQLENQRNNNGTADAATSTGTLTFGISMIEIAIGITVGLFISTLVVYPLGNKKSPHSGNSIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFT0</accession>
    <name>A0A1E3NFT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="11">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_18461</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in pre-mRNA splicing. Binds and is required for the stability of snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site. Involved in cap modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015316.1">
        <property type="nucleotide sequence ID" value="XM_019162008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFT0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFT0"/>
    <dbReference type="GeneID" id="30178695"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990935">
        <property type="term" value="F:splicing factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="14"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="88" mass="9843" checksum="09E8AF13EFA74EBE" modified="2017-01-18" version="1">MAAGQQRTASVPPINQIFKYLQQQKPVSIWLYQQAQVRISGVIKGFDEFMNLVVEDAEEVNVKDPSKRRALGLVLLKGDTITLISSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG03</accession>
    <name>A0A1E3NG03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="3 9">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17573</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3 9"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016188.1">
        <property type="nucleotide sequence ID" value="XM_019161650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG03"/>
    <dbReference type="STRING" id="763406.A0A1E3NG03"/>
    <dbReference type="GeneID" id="30178337"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 9" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 9" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="10">
        <location>
            <begin position="14"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="200"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="508" mass="57459" checksum="FAD15C4B3D5CB7D3" modified="2017-01-18" version="1">MSDNKRFDENTVIVVFGASGDLAKKKTFPALFGLYREGRLAGSTKIIGFARSDLSDQGLRERITPFLSYDKTKQAHVKALEEFLALCSYHRSAYDKPDGFEALEKKVDELDAASGVAQSHRLYYLALPPSVFTVVATNLKKYCYPGSKGHARLIVEKPFGHDLDSSRQLQRDLAPLWSEDELFRIDHYLGKEMVKNLIPVRFSNTCLGSSWNNRYIDSIQITFKETFGTEGRGGYFDSIGIIRDVMQNHLLQVLTLLLMDKPKDFGSESIRDEKVKLLKAIEPIDVHDILVGQYSRSADGSKPAYLDDDTVTPNSRAITFAAIALKVHNDTWDGVPVILKAGKALDESKVEIRIQFKPVHTGIYENSARNELVIRIQPDEAMYLKMNIKVPGVANQVSISELDLTYKDRYSSEFYIPQAYESLIKDCLEDDHSNFVRDDELDVSWKLFTPLLQYLEGPNGPQPALYPYGSRGPDGLADFVKKHGYVYEPRDRYQWPLATPEGINPSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG70</accession>
    <name>A0A1E3NG70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_166706</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016244.1">
        <property type="nucleotide sequence ID" value="XM_019161301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG70"/>
    <dbReference type="STRING" id="763406.A0A1E3NG70"/>
    <dbReference type="GeneID" id="30177988"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16991">
        <property type="entry name" value="ENTH_Ent1_Ent2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF110">
        <property type="entry name" value="EPSIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="9">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="143"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="342"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="498"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="515" mass="58178" checksum="A6FA6ADC917CE53B" modified="2017-01-18" version="1">MATKAVLRSIKNVTNGYSSAQILVRECTSNDQSSPTITQMSAVADLTYKNGTFQDVMDIIDRRLNDKGKNWRHVAKALTLLDYLVRYGSDDVVIWAKDNLYIIKTLREFNVKDFSGNDQGSIIRVKAKELTGLLQDDERLKQERDAAAKSGSRGRKKGGSVRGGRRTGRSSPDDEIYDEDLQRALEKSRMTAEEEERRRRDQSDESLERAIQLSLEEEEMRKKNQNLLNLDDDANAPDVYGFYQQQPQQQDPNQGQIIGYDMFGNPVYGNQPMATGYLENAYQDLAAQQLAAQQLAAQQQQALYLQQQQQLYQQQLAAAANQEVPMQTGSNNPFGQQQLFLQQQQQQQQQQQQQEMLQQQQKEAEEAKRREEELKKQQLLLQQQQQQLLEQQQRVQQLQQQAQSPIKPTNTGSQKINQQYSELNQLLAEGTGIDTFGNVGDARIPAQFTKTGTFINSQGTGFKQVSSQGNPFLDTQFTGVGNATPTGIVPSETGYGFGNQQHQRNNGNPNSLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGN4</accession>
    <name>A0A1E3NGN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 15 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74434</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015841.1">
        <property type="nucleotide sequence ID" value="XM_019164769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGN4"/>
    <dbReference type="STRING" id="763406.A0A1E3NGN4"/>
    <dbReference type="GeneID" id="30181456"/>
    <dbReference type="OrthoDB" id="431715at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000551">
        <property type="entry name" value="UTP15p Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018983">
        <property type="entry name" value="U3_snoRNA-assocProt_15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924:SF26">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924">
        <property type="entry name" value="UTP15 U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09384">
        <property type="entry name" value="UTP15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 15 C-terminal" evidence="6">
        <location>
            <begin position="358"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="505" mass="56419" checksum="F4196237AAEA818F" modified="2017-01-18" version="1">MSAPRERVVTARNPSVPTQISPEQKFWKGFVNSQLVKEHNAITHIHFDPNSPHDFAVTSSTRVQVFSSKTRKVVKNFTRFKDTVYCGEFRNDGKLLVAGDASGLVQIFDAHHPRTLLVTITPTTHPTHITKFHPTINTQLLTCSDDRVARLYDISQSAKPIISFNNHDDYIRSGIFIPTSPNLIATGCYDNNIRIFDVRVGGNDPIVKFNQSSPVEDMLSLTPTNILSAGEGVVKYWDLVAGKLSRTLHNFNKTVTCLSDAGERGILVGSIDGHVKAFDTSTPNWDVKFGWKFGSGVLSCGVSPDFKHLVVGLNSGLLTVRTRKNNNDNISATTATSNNLKFKGDKSHGFARFMRGSDFKGEGEHHVIDEKVNHKKLRSFERDLNGFRWSDALNSALLDGLDTEATITCLEELKKKGKVRIALANRDEASLEPLLTWCLKFIDDPRNVSIIADYVICILEMYDELISRKPVLEELVLNLQKRVEIELKKAQDSERIIGMLELLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGR6</accession>
    <name>A0A1E3NGR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_36192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016427.1">
        <property type="nucleotide sequence ID" value="XM_019162992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGR6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGR6"/>
    <dbReference type="GeneID" id="30179679"/>
    <dbReference type="OrthoDB" id="2427554at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF0">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="461"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="3">
        <location>
            <begin position="46"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="648"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="692"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="704" mass="78903" checksum="74C6A786B6CF1AA5" modified="2017-01-18" version="1">MYHEPTPARLFIYDLILWAFCVVFDCFFREIRSRGGFKVPKKDSLIFVAAPHANQFVDPIILMSQVKKASGRRISCLIAEKSHRRKFIGLVSRSQLSIPVKRAQDNLKLKKGTIKLDPENELHIIGEGTSFLKDCHPKGLLSLPKSLGTGPIDKVDSDTELYLKKPIKFASEKAEAEARLLLESGSIYKAAEKIDQSEVYAQVFEHLAHGHCIGVFSEGGSHDRPDLLPLKAGVALMALGAMDANPGLNVKIIPVGMNYFHPHKFRSRALVEFGDPIEIDQSLTDMYADSKTSNEAVRILLGEITKGVKAVTVTCPDFESLSVVQAASRLYSDQFSNKLSIASIVEMNRRLLNGFLHFKDDPRIKSLKNEILKYNEDLKSMKVPDHLVGKAIVKSKLSILLDLVQTFSQVIILGILALPGIIFFSPVFVATKKISEKKRKEALAGSTVKIKGNDVIATWKILVSMGFAPILYTLYSLLGIRIVHIYYPSASKIFAFLTFYAFSASVTYSALIFGDKGMDLFKESKAAWLMLTNQQGFTDLKTKRDKLASDITQTINELGPQLFPDFSILEYEKKLEMKKLLKDERKKRHLEKLEKGLTQEEAIEEMKTQELRRRRTEKKLNQKANTTSDSGSVTDKKIRMTQNTPIINGDDNSSSAGLSSTSLSDYDDTSSESEFSHSGSDKYKTLGLIKDQIIKENRERDELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH41</accession>
    <name>A0A1E3NH41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_13191</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016588.1">
        <property type="nucleotide sequence ID" value="XM_019160026.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NH41"/>
    <dbReference type="GeneID" id="30176713"/>
    <dbReference type="OrthoDB" id="5330228at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052583">
        <property type="entry name" value="ATP-helicase/E3_Ub-Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059033">
        <property type="entry name" value="C144_05_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26021">
        <property type="entry name" value="Ferritin_C144_05"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="331"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="1202"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1272"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1407"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1272"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1418"/>
            <end position="1427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1589" mass="182432" checksum="6B7F0B63AD02887A" modified="2017-01-18" version="1">MPVSFVLLAKDIRYENLPLLLDSRSPAVEAASGEDEIELVSYRSEISLKHPKILDDGEYAQVEGMFAGSNTVRISHDVAVKAKKPSQAADGILLQFQQRTLKPLLVLSFRPEYRQLVLLIHFFLHQTKLRSTPYVRTVPFHLRNVELTLDRTTRTFNIRFSLCFSISVRESIYAFLPPDTLSSLNRMAELLFIPRPTAYQVSFNSFSAAPSVSSFYSLITDHTRRAHTLETQMLNLPGVYKTLMPFQVDSLKWMLSHEGVELASCDGGIKTRGKPFPRDANDETTSEILDTIIPGWTRLSSYADQSKLYWYNPYNGSLCTSAFVKKYLESIHFVRPPAKGFLCEEMGLGKTLEITTLIKANPRVLVDGGLKSDLLEPSRKIKQSKTTLIVCPETILNQWYGEITSICSDLTVNMYKGVSLLEEYDDSLTPTMIANELSSYDIVLTSYNTLARELSRAIFIPTSRPKRKTSSYDRIDYSSPLMLLEFYRLVLDEAQLASISISKVAHFSRIIPRIHTWCVSGTLIRKNLEDLHSLIMSQRLNPLDNLNIQQWNQLPRHFFDRLFSRVCLRHTKEMVGSQVRLPKQHRIMLRSPFSTIESDNYHDLFNRFLEQVGLNDNGEPIAEGFDLERSRMGMSVWCNKLRMACCHALLNGSQIRRNMALDNAAGGNTKSAKGKNKNDADLIIGTLSDVLSDLVNSNEAESHNCFFSYAKNYIKMGKIHEFLRDPAKSIEVFLSVIDVVREKLSCYQRYYDSEATSDRRKNWTVRIRNLLEFLHQSYFMLASAHYQHYRPMKPLPKSFRDLNESVLDEKKEEKAEDVEVVDVETLSEEEKKHYHLENEYYAKADEILNQLLEEPLKKTNEMITKLGKTFSKFSVYKVKEIPTIKPIEEDETFSVEIKPEKHEEYELPLICEYFEDLTSDMKNHATSLEVSFVLNRSQEAMEQLNEQSTIINFWFKKLIEYQKIPVVKNDETDKTGEEYGLSLISQEESQAYIDQLQLILDDREKAINSTEDTLAYKSNNFKMQRIFNDSISSKSPLSLELEKLRKYYIPQGTLNPRYSYRTAILELVGELQSYTLDSYKHIQLSGLIAMLKAEMTKQMKNVKHMRVKLFDVLNDAFNSKVSYFKALQVRSDTLVNFTPEKIGDSPQYSALVELETVKKELAKDDSKLRSLNARLNYLRSLNSKRQTEGNQGENDNLKEDSCVICRYRILVGTLTPCGHKYCRECLTEWMKTKKVCPLCQKKLRVDELYNFTYSRGGLKGDVVESLHDHKEEVAPVEDKENENHLAKGESVKNEDSTLNEEENLADDDLDRLKLLQNRRLFEKDMDFVYQGLPTAQLREISNIGLKRNYGTKVDMIIRQAKYLVTKDPSVQILVFSQWNAFLLLLGRAMNYEGVKFKSWMNQKAAVSNETGMGRTSRKNNSSNSKLNQDITDFKRDPSITCFLLNTIAQAAGLTFTNASHVFLCEPMINLSFELQAVNRIHRIGQTKETSVWNFIIEGTIEESIAYLGTKKRIQAAKVRKSVMNTEQEERNEVAESADEIDDDVLEAKELTKVNDTSRKDGEVIPDDDLWAAFFAARSVKVIGSVYTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH61</accession>
    <name>A0A1E3NH61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_17892</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016539.1">
        <property type="nucleotide sequence ID" value="XM_019161796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH61"/>
    <dbReference type="STRING" id="763406.A0A1E3NH61"/>
    <dbReference type="GeneID" id="30178483"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="59432" checksum="0948D08F67B52E9C" modified="2017-01-18" version="1">MTGGKDNNANVVSRDINSDSKESNYEVSVNPQLSTILSLNDEVNNMDIKDMDEAYKFLDAETEALELDPAYEKKLVRKIDFHIMTIICGLMAVQMMDKTTNSYAVLMGMRKDLKMTSEEYSWVGSSFYLGYLVFEYPANLLLQKMPLSKTLGLAIVLWGVVLMCHAACKTAATFLLCRTILGIFEGFMNPSYIIMTSQWYRKEEQFMRTCLWYGFQGLGTLVGASIAYGLQVHRAGYHAFASWKCFYIITGAITVFLGVVSALHVPDVPVKAWFLNKKDKMYAIERSKSNQQGFGNRKFKKYQLVEAFTDPITYCIFIYAMSYAIPNGGLNNFGSILLNKDFGFSSQQSSLMNMPGGGIDIIFPPAVALINHFWLKDFKIISMVIINLLCVVGTCLLAFTDHRGSKLTGYYTLYISTTNMAGVASIVSSNVAGHTKKITASTVFLVGYCVGNMIGPQTFRESQAPGYAGAKTAMLVSYCAGAVALFVIYWIYFKRNKDRAAERKQAEEVIPADRIAFADLTDLQNPEFVYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH63</accession>
    <name>A0A1E3NH63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MATE efflux family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_17939</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016584.1">
        <property type="nucleotide sequence ID" value="XM_019161816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH63"/>
    <dbReference type="STRING" id="763406.A0A1E3NH63"/>
    <dbReference type="GeneID" id="30178503"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="53936" checksum="4E71F3993BABEDC6" modified="2017-01-18" version="1">MTSGQNTLVDVRLHHENYGSTVSPAIVDLVGGLDEEQGLQPGTKREKSDSLMNEIKVISFSSAPLAVTFFLQYSLAVVSIFTISKIGKKELAAVSLATMTFNITSSIFTGMSTCLETLCSQAYGAKNYRLVGLYFQRCFALIAVFNIPLVLIWWKSGYLLKFIVPDPALADLAQSYLRVMSFSTPAYIFFETAKRYLQAQNIFAAGQYVLLLVAPINILLNYLLVWSEVIGIGFLGAPLATTISYWLCAVLLLIYIVVIDGSQCWFGFDLRGCFQNWSSILPLALNGTAMLLSEFVAFEILTLSSARFGTSTLAAQSIASTIATLCFQIPFSASVASATRIASLIGAGKQSEAIISTNAAYTIGLLLSVFNFSILFFPRTKIAELFTDDEKVINIAVTILGVLSINQLWDCPNIIGAGILRAQGRQAVGSKLNIVAYYIVAVPVALYLGFKVNMQAKGLWIGLGLGVFCLALSEFAMVFISDWNVISMEADKRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHB2</accession>
    <name>A0A1E3NHB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">Alpha-1,3/1,6-mannosyltransferase ALG2</fullName>
            <ecNumber evidence="6 16">2.4.1.132</ecNumber>
            <ecNumber evidence="5 16">2.4.1.257</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_11979</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 16">Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="EC" id="2.4.1.132"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14 16">
            <dbReference type="Rhea" id="RHEA:29516"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29519"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="EC" id="2.4.1.257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15 16">
            <dbReference type="Rhea" id="RHEA:29520"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.132" evidence="6 16"/>
    <dbReference type="EC" id="2.4.1.257" evidence="5 16"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016630.1">
        <property type="nucleotide sequence ID" value="XM_019159862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHB2"/>
    <dbReference type="GeneID" id="30176549"/>
    <dbReference type="OrthoDB" id="448893at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004378">
        <property type="term" value="F:GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102704">
        <property type="term" value="F:GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033164">
        <property type="term" value="F:initiation-specific glycolipid 1,6-alpha-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03805">
        <property type="entry name" value="GT4_ALG2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027054">
        <property type="entry name" value="ALG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918">
        <property type="entry name" value="ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918:SF1">
        <property type="entry name" value="ALPHA-1,3_1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="18">
        <location>
            <begin position="25"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="17">
        <location>
            <begin position="314"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ45517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="471" mass="53399" checksum="75FA682F5C8A8D1A" modified="2017-01-18" version="1">MTVVDTKQQQARKPNIAFIHPDLGIGGAERLIVDAAMALQSVGNSVTIYTSHCDKTHCFEEVKTGLLDVHVYGDFLPTNFKGRFNIVFAFLRQLYLSVQLILSLKIFKYDVLILDQLSYCIPLLHLFSWNTRILFYCHFPDKLLAPHTSVLRQVYRFLFDAVEEISTSYADQIVVNSEFTKSVVRQTFKLLAGQPLEVVYPCVSTDETTFAPSSESLEIVDKLLGNNKYFLSINRFEKKKNIQLAIQSFASYTSATNDSFQSVVISGGYDPRVSENVEYLNELKKICDKLSLSYKVYSPSSVNFSGQLNENEVPNVIFLTSISTDLKNALISKCDILLYTPTNEHFGIVPLEAMRMGKPVLADRSGGPLETIVDYSDDKVNYTGYTISSDVSGWTKALEQVKRLPKEDLKQLSARTKKRVDEKFSLVALRNKLVKVIHQMMLKDSPHSWVTHVVFPILFVVGVTFLYRSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHL8</accession>
    <name>A0A1E3NHL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016732.1">
        <property type="nucleotide sequence ID" value="XM_019164388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHL8"/>
    <dbReference type="GeneID" id="30181075"/>
    <dbReference type="OrthoDB" id="74178at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052602">
        <property type="entry name" value="Growth_transcription_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022092">
        <property type="entry name" value="TMF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515:SF1">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR TMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12329">
        <property type="entry name" value="TMF_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="6">
        <location>
            <begin position="721"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="582"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="204"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="493"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="730"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="845" mass="95289" checksum="6BD22BC3DCD6F4EB" modified="2017-01-18" version="1">MDEESGEVNTTPFSTEADVESVTNAQTQQPLEKPNPSEKVEKDSEVDNMLQNPEITKEIQIIEQPTSTPSILATEKTGEKKKKLSLQERLALAAQKKSKNEPKPKRKLIGEDKQKEAASDTIADEKSSSSTIVQQSNTAAVDYDLEDLYRMIPAELAGQRDQLVSHLKVYVAKLIDNNSKSYVTRISKLENELSSVKKAKPVSLSSTESELLKKLEEKEQQINELIEEGNKLSKKELALNQSLKKMKIREAELEEDVEQYEKSTEELNHKVELLDKKVVDFEDNERALVEEKLALQTLRTKYDSLVRANESLTDELKEIKFSKLDVQLEKALAELKEERTAHSKILEKYEKLNALYNQTSEEKNTLISDLEGQLRDEKAKCSDVARESEAEIKRLGQKIEALRFQSESTIPTDKSTEDMEMLQSQYDQAKENWKLIESSYLKKISNFEVQLEDLRKTNIVYSKKIKVLSNDLKQKSTSNSELQELESNLVFEVNLLKKKNASLMTANQTLEDNIEQLKEEFAKEKESFEKKVQTLEEEKEGLESSLKLRTNDFTSSQHMNQNSFYLQDLSSSSSLNHMKAMNNSTMGRSTSSKRFSISVGESSTTPRLSTSNSSFSIHKLNGMASMSAQDKILRHQNSTISFNSNDMQQQPLGINTTSGANNELISGGVSGTTPFLLESPSINNASQDLASLNDDIPLGMDAESERVSTLNGGTDTPNIGSGGGLNIQLIKKLSAHIRMLELEVTTLKDETKTLEREKDAASEEIVRLIQDNSQVQLVRDEVTAKEAEVSKLQKNYERVLILLGEKEERVGELTADVEDLKDMLRQQVQQMVDMQEKIDKISSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHN3</accession>
    <name>A0A1E3NHN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_36433</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016181.1">
        <property type="nucleotide sequence ID" value="XM_019163006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHN3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHN3"/>
    <dbReference type="GeneID" id="30179693"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DPH-type MB" evidence="8">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="72" mass="8458" checksum="1DF4FF381C5B39CE" modified="2017-01-18" version="1">MSETIYDQIEIEDFTFDPDTQLFTYPCPCGDRFQISIYDMQEEENIAVCPSCSLMVEIIFEPSDLEEYLDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHN9</accession>
    <name>A0A1E3NHN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_16947</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016765.1">
        <property type="nucleotide sequence ID" value="XM_019161404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHN9"/>
    <dbReference type="GeneID" id="30178091"/>
    <dbReference type="OrthoDB" id="387657at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052712">
        <property type="term" value="F:inositol phosphosphingolipid phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072711">
        <property type="term" value="P:cellular response to hydroxyurea"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000073">
        <property type="entry name" value="Inositol phosphosphingolipid phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="14">
        <location>
            <begin position="17"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="464" mass="52127" checksum="DF978D2948AE979E" modified="2017-01-18" version="1">MALSDNGSGAVNTFKLLTFNTWGLKYVSTFRKERLVAIADRLAKSDVNLFTSSTSPPSIVAGTSAPVAVSPPSSSSDVDDYDIVALQEVWSKEDWEYLDRVCAAKYPFRRWFSSGILSGPGLAVLSKFPIKKTFLYRFPVNGRPSAFFRGDWYVGKSVAVTILVLSDETTVALLNSHMHAPYAASGDAAYEAHRSCQAWEISVIVEELKLAGHAVILVGDLNSRPGSLPYRILEIDAGLKDSWELLNGQTDLELVKSMDPWEQIIRAATTCDSILNTWRAHRRPDEACRLDYALIDSNLLEPVQASVRFTEKIPSIGSYSDHFAYSATFRFKKKVISDSEATATTVLATSRSGSNEKLLQVYFDLKDLVSEYMETTLVWQRKWRMWHFVISFAVTLAFLPVITVVSYRAPWSSILFYFFGCIIFVTGVVNGMISFLFGRYELRNMRELILEVDDRLNYVKAFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHP2</accession>
    <name>A0A1E3NHP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_16962</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in the regulation of membrane traffic through the trans-Golgi network.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016783.1">
        <property type="nucleotide sequence ID" value="XM_019161410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHP2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHP2"/>
    <dbReference type="GeneID" id="30178097"/>
    <dbReference type="OrthoDB" id="2018246at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16998">
        <property type="entry name" value="VHS_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000008">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000024">
        <property type="entry name" value="VHS domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052653">
        <property type="entry name" value="ARF-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="8">
        <location>
            <begin position="21"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="10">
        <location>
            <begin position="184"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="9">
        <location>
            <begin position="457"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="62868" checksum="706C34CAE312D5B0" modified="2017-01-18" version="1">MSSLTTLPGSSGKLLRRIHRACRPTLDEPNIALNLEICDLVNSKQASLPREACIAVAKLINSRDPQVSELALTLLDYLVKNCGYPVHLQISRKEFLNELVKRFPERPPPAYSRVQRLILGTLAEWVQTLCKTSRYKDDLTYIKDMYRLLRSKGYDFPAVKREDAAVLNPSDNLKSIEELQREERIAQSAKLQELIRRGRPQDLKEANDLMKIMSGFKEDEALEETKQKVYDDLEKLNRKIDLLDEMLNNASNSGKLNKNDDTLQDLISTVKVAQPRVQKIIQEEEGSGDVAKLLLLNDKINSSLIKIAKLQGEDISNISGPASRAPQSINLIDFDDDEPESQAGNSSEAAPNTNDAINDLLGDLGGLSFDNSSSNNANSQFGVGAINLLGTPEPQGASKPLSNLDILSGFNTPQQQQPQQKSQFPLVAASHSSTTSVSTPTQLDPFGFDFGSMPAPVSTSTLVLFSDERITVEYTVSKKQPAFEATFFVSNNTPNQLSNLQLSLAVTKSFELSLNPPSSNALGPNSKKGIKQNVTVKSRSGESFNSLKFKYKLAYLSNGSPVEDSGVASISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIG2</accession>
    <name>A0A1E3NIG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein IVY1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_59513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017032.1">
        <property type="nucleotide sequence ID" value="XM_019163372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIG2"/>
    <dbReference type="STRING" id="763406.A0A1E3NIG2"/>
    <dbReference type="GeneID" id="30180059"/>
    <dbReference type="OrthoDB" id="5594612at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037470">
        <property type="entry name" value="IVY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38407">
        <property type="entry name" value="PROTEIN IVY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38407:SF1">
        <property type="entry name" value="PROTEIN IVY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35445" checksum="3AF1B5F04F912AC3" modified="2017-01-18" version="1">MALDVLVTKKDVSESVEQMQELSRCCEALSQKLKEASDAFGDFGAVIEKISRSKGSGEHCNALSTFSNYQYLISNQHRYLGELLQREFGDKIGDIKNEYEGRNRVRRDEFQSEYKTLVKELKSSEVANSKLRKGKIRNLVSYKTNLASLTSKLDNIDRVYHDYYVDSYGLIEEASGRIVERARSVVRQESVVFGKLAEKTRPGNGLDVLFTEDVDADVDVDADAEAIAVDEDEDVEDGDTTIREERDEDEAAVRAADDVEDDDDDDMSRRDERYMQQAVELLRQEQPEPAGDGSVDRASEAAKASEADADQPMPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIQ6</accession>
    <name>A0A1E3NIQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Early meiotic induction protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_168427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016336.1">
        <property type="nucleotide sequence ID" value="XM_019161358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIQ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIQ6"/>
    <dbReference type="GeneID" id="30178045"/>
    <dbReference type="OrthoDB" id="2017405at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18067" checksum="4A20268E99E0D715" modified="2017-01-18" version="1">MFWSKKEETSATPTATVPETKDDDFHTPPNDDGNELSNMESIINDVIGEGKKGSNTLRDKASSRAESLELHKFPKEMSCMTALDELMQCMSLGGQIRSYYRYGDLTLCDKQSEKLNFCFSNSLNTEDVKEVNVQDFYKKRLVEQLKRGSSEDIWESRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ02</accession>
    <name>A0A1E3NJ02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_73589</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017228.1">
        <property type="nucleotide sequence ID" value="XM_019164525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ02"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ02"/>
    <dbReference type="GeneID" id="30181212"/>
    <dbReference type="OrthoDB" id="5364946at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034513">
        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000493">
        <property type="term" value="P:box H/ACA snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000454">
        <property type="term" value="P:snoRNA guided rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000015">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit NHP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="9">
        <location>
            <begin position="34"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="148" mass="15941" checksum="1D962E24391992E0" modified="2017-01-18" version="1">MGKKEGSETDNYDTKLPYVLPFAQPLASKKLNKKLLKTVKKAAKAKHVKRGVKEVVKALRKGEKGLVIIAGDISPGDVISHIPVLCEDNGVQYIFVPSKEDLGGAGGTKRPTSCIMVVPGGGKKGDKSGKTEEYKESYDEISKEIKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ23</accession>
    <name>A0A1E3NJ23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_17349</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017260.1">
        <property type="nucleotide sequence ID" value="XM_019161556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ23"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ23"/>
    <dbReference type="GeneID" id="30178243"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="328" mass="37391" checksum="89368A9B51831214" modified="2017-01-18" version="1">MSRKRKKQEDDAGERKQRKVDDSYNEDEMNTIYNNPKQFQGVMRKYWAQRHNLFCKYDEGILLTKELWFSVTPERISKYVARAIYEQLAAARARQAQPATDSPFRILDGFCGGGGNITQFLQYPDCVVYAVDNNHRHLECTKNNALVYCGSESVDERLRLLPMDWNYADPDAPAAAADDPDHSDSDSDSDSDSDSDDHDLEDAVYASRSQSLRSLRELAALRFDCVFGSPPWGGPEYIKADKYNLDCLQPFDLTKMLLVLRTYSEFVCLFLPKNIDIEQLVAATREAYLQHPPQSLGGGAKVKVKVKVVHMFTFQREKGVLVTWSVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJC8</accession>
    <name>A0A1E3NJC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_154278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017289.1">
        <property type="nucleotide sequence ID" value="XM_019160823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJC8"/>
    <dbReference type="GeneID" id="30177510"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="67" mass="7389" checksum="5E3038DB89F25E71" modified="2017-01-18" version="1">MPRGASCPLRHSRAQQSRAAQSPLEQTAAPSTPEHRRNSPKRLWMQALLPGMPLLFCVLARTRGAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJD6</accession>
    <name>A0A1E3NJD6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_58558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017299.1">
        <property type="nucleotide sequence ID" value="XM_019163306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJD6"/>
    <dbReference type="GeneID" id="30179993"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="72" mass="8349" checksum="D86598C39255EB9F" modified="2017-01-18" version="1">MLKGEPTYGPELQMSTLQAQRYLPKKIMEGRSSNREIRKRQDDSRPSHSSLMLALAVAMVTVHSFEYTHYCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJF2</accession>
    <name>A0A1E3NJF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_11255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017354.1">
        <property type="nucleotide sequence ID" value="XM_019159775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJF2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJF2"/>
    <dbReference type="GeneID" id="30176462"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="50586" checksum="60CE651C8B2F9D78" modified="2017-01-18" version="1">MDLYSRLNPSSKIGNTSYHLVQLTKDLLEELDSKKGQPATPTPTPTPTPAVPSEHDRLLRKYDVKASENYQFKSIDELATPYLTTPSATYKIRQQNHSNCVMLLHNNVNFLNFDNYLILERQNTHQLRQMKLSFKDVPIKELNSIADIKQLHSDNEDDHDCLTVQQLNKIYASSKKLLENTPISISEFDTLILQSGYVPHYNSLAKIHTNLETMILNLLLSCILEVAEDINAELLPAQAADVLRRTYFKILEKFRLEKNHLILRIVTNVFLRYVQPSSNAAAVAELPPLDEADQYVAYSLIATGGYLLLHNKIIRFLTMTILEKEKTILLDDLLIQIRLNLPVNYLPSFEISEILSGFSYTQKLDNGQLEIHHLTVDQLSAYKTPQERFSRLFGLKSQWTLEELQPFVAPLNAKGIKIDKFCLKYCRVKKTKNRTVLMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJG3</accession>
    <name>A0A1E3NJG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017398.1">
        <property type="nucleotide sequence ID" value="XM_019164177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJG3"/>
    <dbReference type="GeneID" id="30180864"/>
    <dbReference type="OrthoDB" id="4096201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="457" mass="50693" checksum="21140F5A4193681B" modified="2017-01-18" version="1">MSISIKRSITSSHQDSGLPKRQCLVNSNSVVNRLCAYRNAGRLTRDGTVDASSSSDDNDDEDRDGDDDKSDVEQAAERSNALKVKSIRFADSICGTPIDSRLSKAVSQYSDDEDDEDYDNGNGGEKDSVKIAEARHENEHAARASGRRDSSSAHRKEEGLCSSGKSSPFSEGRAFSLNRKRATSTNDILNVNYVSGHGLLNNKRYSVNISDVVSSIPNSSENFHSFNLSNKALKHSGNIPRCDFIARSRCFEYLVGAIDEAWARYCDSTSYDEDLAYGYDDSKSNRGIANTRNNDTDCNISAGGAGAAVANTPSSNNYSSDDDEGYKTEFSATTTVTEYDSDFHNQGGNKFRSFSMINNTLNGNSTKSNNNNNNRRVSEVPENVRLQELKDRFTKSKYYLEDLVDSDLHSDCVAFWTKWDLIKYSIVDFVEEDEEDDDIERKIDELEAGRFAGSFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJI1</accession>
    <name>A0A1E3NJI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Pre-rRNA-processing protein IPI3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72370</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the WD repeat IPI3/WDR18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017402.1">
        <property type="nucleotide sequence ID" value="XM_019164179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJI1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJI1"/>
    <dbReference type="GeneID" id="30180866"/>
    <dbReference type="OrthoDB" id="756370at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045227">
        <property type="entry name" value="WDR18/Ipi3/RID3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763">
        <property type="entry name" value="WD-REPEAT PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="488" mass="53401" checksum="0AB790A760B499B2" modified="2017-01-18" version="1">MDELVYYNATGKPEDKKNLTSFSYLSSLHSTKIYHQYRQSPSSPNGSVNLSIGHGSHLLVALPEKPILHVYLHGKESPEQIIPLPEILTCLTCYRDSEDPQKPTLLLGGSVSGRLYVWSINSGLLLAVKQPHYQPLTHIAAYSGFIATGSKDSRIVVHTLNTIFMHATGIDTDTNDKPFATITDNTLPITSLVFNRGLNNDVKLVSSSLDSTVRMYSLAAASGAELLTTIVATGPVTALAADPAFRALYLGLADGQIRSVPLFRVNPRTKVVEAVGGMGRVVTLKPDVDNLETFTCHQDQHKPAADVAVTQLKVSFDGTLLVSGDSRGSLFVIDITTKQVRKKLKELVGEISTLELWTVDLADARFDNTGVDKNLKRTIPVLKRSVAESADLSALKLSLKLTAEPQPTTPSPWDFDSWIDQVQSEEKAFANLSAVDSHTFGNTGFSLNIDKNTIRDLNKELQDKDRTYAELKAKYDELLSEYTAAVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJQ2</accession>
    <name>A0A1E3NJQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_72418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017454.1">
        <property type="nucleotide sequence ID" value="XM_019164192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJQ2"/>
    <dbReference type="GeneID" id="30180879"/>
    <dbReference type="OrthoDB" id="189997at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="502" mass="57233" checksum="7C9690D11C6A3B04" modified="2017-01-18" version="1">MLPSCIICKKRKKKCDFNLPSCKHCESMGTQCEYYDEGLDNNVPREYLKSLNDNVIALRNEIEKYRKKINKRTSQNGYTVDKKNRLYEVIKNGTLLEGTNSELHYFGPCSLVSMIYMSSMMLDLRITSLDRVSILDHKSIPPVKMTFDYGMITSDHSKVLITNYLTNIYPSNPLLSESFFHFDLMVKNYPDKKQLFILLILLISSAQIMRKRTDFVPIKVMLQQKVLEIMKTKLSREDGDSILAFLLYALYESFDPETGKSMWKTLSSACAIAERLQLKNLDNTKPLPGLIACTIPRSQFLKVLVFLDSEMSLCLGNSPSISLSKNQILNLKDLSLQSYFLQIFEKVQFYNTLYANSSGCDIVRLFESPMLTTQNDPNIWILASPLLSHNCTSCEAYFSTVVMNILNASIAIIGDYDLRIKGTGITLYWVALSKISTAVLNLILIKKLYYSNYFGLLEKSTVVDNIILGQRLVSQISSQWYSANSIKSFIDMACAIHKLQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJU1</accession>
    <name>A0A1E3NJU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_11408</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase IV family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017520.1">
        <property type="nucleotide sequence ID" value="XM_019159787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJU1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJU1"/>
    <dbReference type="GeneID" id="30176474"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF10">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="173" mass="19451" checksum="ED5649CAFE921733" modified="2017-01-18" version="1">MSYSGKSLARALGVHGSVVRRNGARHVQLRNTAAAARLFYSTGAKTESKHKPAADAADDAAAEDDEYYVDVSDLDKRWPSLAFEAQQDIISYLNVKQEFDWRYLSPPEKRAIHYIAYGPWGARDTKVMTGAEFVFKLMTNMLLFSVLGFSMLNYAVDQEKIADLEHSEQPPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJV0</accession>
    <name>A0A1E3NJV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED19</name>
        <name evidence="11" type="ORF">PICMEDRAFT_17112</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016961.1">
        <property type="nucleotide sequence ID" value="XM_019161469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJV0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJV0"/>
    <dbReference type="GeneID" id="30178156"/>
    <dbReference type="OrthoDB" id="2160599at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08633">
        <property type="entry name" value="Rox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="167" mass="18831" checksum="37BD3BD2FCCE02BF" modified="2017-01-18" version="1">MSSKSSSTGNYYFIDSNETCQPAQPTLRADVIRMYNIQDLAQALTRTNPDGSKGMKLRKSYKSHILDLPGKHNIPTEDKSFTQIALMPENPDFAKPQIEPFSLEYLDKILNLEKTGANGIPGFDPSKLALSNMDFSDLKKEKKRKPGASLSPEQQEVKRRHVQVKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJX2</accession>
    <name>A0A1E3NJX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017558.1">
        <property type="nucleotide sequence ID" value="XM_019164216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJX2"/>
    <dbReference type="GeneID" id="30180903"/>
    <dbReference type="OrthoDB" id="4046837at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="543" mass="60996" checksum="FC4CE1A32C726A95" modified="2017-01-18" version="1">MLGRHTVVRGFCQAQKRAFSLSRRALIDVNSHHSVSDTINHLQTQGESDPDLKNGHSYLDIINHHASRLEKQGPESAEEANKEYNDLYTLSLQLSKLLESTPDVSEELKKNILNSLIEKFTKYNYAVSTLAFKKLLNDKQNLSQGSVSELICHNPGRVNSSWDVYESLKPEEPHDKILVAILKKLLYGDKVEIKEGLADVDVEKVIKIFQVYERIGNKDLIDEQSLLKLVTDLMKLDCSALITQMVIPSSIFEKIINDPENFDLKNVDYLHFYEASISNGVSLSGNALLKSLMPISKLQTSTVLESENLKKVKEKLGVSILQLAPLPDVVDEIREQIQELGLDDEAPVMIDLMKSAGFYSNDLPTAIKYFQHYQSKVPDGTVQQNDLKTTMSLVFVYSCVSSNDPKNVNVAEALVPQSPLPAANNLASLVLFHGWFGDSDKAFDIYNQSLDLYLKPLEEFVSERGMLVQALATVSLLGKEIGLAKLIKDKSLENKLIDETCEIKISNLFKEYGDKLEECKDSDGAFRESMKKIFLRTIIDFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK05</accession>
    <name>A0A1E3NK05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Thiol-specific antioxidant protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_16341</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Also involved in the correct composition of the hyphal cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017563.1">
        <property type="nucleotide sequence ID" value="XM_019161173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK05"/>
    <dbReference type="STRING" id="763406.A0A1E3NK05"/>
    <dbReference type="GeneID" id="30177860"/>
    <dbReference type="OrthoDB" id="185659at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061692">
        <property type="term" value="P:cellular detoxification of hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900745">
        <property type="term" value="P:positive regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF171">
        <property type="entry name" value="PEROXIREDOXIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 13" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="8 13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="4"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="14">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="196" mass="21566" checksum="0C20E626322A9928" modified="2017-01-18" version="1">MVVPTIQAPAPAFKKTAVIGGAFEEVSLEQYKGKWVLLAFFPMAFTFVCPTEIIAYSEKAKEFKDKGAEVLFASTDSEYSLLAWTNVARADGGLGPLNIPLIADRNHSLAKDYGVLVEDEGITFRGIFLIDPKGTLRQITINDLPVGRSVDESLRLLEAFQFTDKYGEVCPANWTPGADTIVPEPENSKKYFSKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK59</accession>
    <name>A0A1E3NK59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_12184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016836.1">
        <property type="nucleotide sequence ID" value="XM_019159896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK59"/>
    <dbReference type="STRING" id="763406.A0A1E3NK59"/>
    <dbReference type="GeneID" id="30176583"/>
    <dbReference type="OrthoDB" id="19159at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034586">
        <property type="entry name" value="Bfa1/Byr4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="597" mass="69503" checksum="3D968A58C9EAB26E" modified="2017-01-18" version="1">MEADFQKLKLRSSNLSTVHEFDERSTLTKENVKLLEKQKMLQKYMDDTGLSAGNFEEDRFFDEDYGDDFDDDADGEGNNGFDFDAQIKKQIESQGQRKQHVKQQEQEWPQSRQRKSALRSSMATPIGSHVYTLVDKPTVEHGSISSFSSRTPSSVQSQYSESDTENSDYAADYDDFEEVGKTLNLQEKFRRKQQEAKQHVEEERLLRRKQNTNSRSKLPRSDSRLAQHYDVDDITNDADFEDLNYIDPSKIYRFKANPSPSSHGYLDRKKSLPVMRVVTSVAGSPKKVKKYLSSVDMNSKLDKYPKQSYPVYDRHGNNNHERELDELDDLDDFDLTITLSDYKKLKRKSKKIDFSQYVETPSQHQYRQHRESRDSRYIPQRLSSSGDYSSKNVLFSTPEDSKTARLTKEGKIKLIRSLGKPMVRKVMPAHIYGEIVYDPQLKKWCGNEEDLARFETLNYSKPQLITKKQSMPQMIGNMVYDDKKLRWVSVTGNYEDDPFGEDFDTIMNGSEEPERTKPGILKPSGRGVSGKLVPSESSMSLADHLKKKDSYFKVTPEMYKTWKTEEGRWVRKVGNWFPYDENTHKFKYELKTFLNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK85</accession>
    <name>A0A1E3NK85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_16398</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku70 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017643.1">
        <property type="nucleotide sequence ID" value="XM_019161189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK85"/>
    <dbReference type="STRING" id="763406.A0A1E3NK85"/>
    <dbReference type="GeneID" id="30177876"/>
    <dbReference type="OrthoDB" id="3249161at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003033">
        <property type="entry name" value="Ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Ku" evidence="17">
        <location>
            <begin position="324"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ46530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="673" mass="77526" checksum="3665FFBBB23128B7" modified="2017-01-18" version="1">MNKSSIDDYDENEDEAVNNFKIHEGIILAVHLTNSLTEKMTAIFSTFLNLLITLTKTMPNTGLGLYIFNCAKKSEASKDGNVDTPEGVFRVFRLQDLNQGMLKTLDRYLKNAIPTETVVNLSFNNTEKWNSLLPIKDVDVNASFGPSLYGMLQQTLLDFTNIPIRTEEYTSKKVFFFTDCQTPFNGKESIKQKIHNKLRDLNDFKITIYPFILKNEESEDAKMASHQIDEFKQLFDFPMDTDLEAKKYLPAISEISLETLEEKIVKHATVRRTAFQCPLIFGDVKIIVRGINPFTTTEWKKVKFYNIENRLKYVKKRNIPNSGDNEADTADIKKVYQVADQYMGMEEKIQTECMKFGESEKPILHVVGTRKFKYFNSSSIISKAVFVIAGDDEELESSNSLDNFAALYRSLCKKKMMILCWGMPRKTSYPRFFYLIPTGITENFGLKFEKYPHALAMVEVPFGNEVRKAPEYINKLDSLSELDDTHILDKLIEKLTLDGFEAFSNPSLSWKFKVMEDHILQREVPRSETRAESNEEEEEESSNFGEQQLELDEMYQQMLLLRRKINKDESLQSLVKELQSRYNRVSNYNELKRTSDDVKSEKLSAKKSRGLKFSDPLTDAKAIIFYRECGLQNCTNDVLRNYIKSKGGLIKIGKTKGDMIDNVVAFLKSNQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKL4</accession>
    <name>A0A1E3NKL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73363</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016986.1">
        <property type="nucleotide sequence ID" value="XM_019164457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKL4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKL4"/>
    <dbReference type="GeneID" id="30181144"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03755">
        <property type="entry name" value="proteasome_alpha_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="270" mass="29615" checksum="284CB108E1D29092" modified="2017-01-18" version="1">MSGYDSALSIFSSTNTTTTLFEVNNCISNSPDGHVFQVEYALEAVKRGTCAVGVKGADTVVLACERRTTLKLQDPRINPTKINKIDYHIQLAFAGLNADARVLIDKARVEAQSHKLTLEDSVSVEYLTKYVAGVQQRYTQSGGTRPFGISTLIAGFDENDQVPKLYQTEPSGIFSAWKAQSIGRSSKTVREFLEKNYPADEPLDEAGTVKLAIQGLLEVVQTGAKNIELSVMKPGAAPRELSNEEIEEIVKEIEEEKAAEAEKKKPRSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKR2</accession>
    <name>A0A1E3NKR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017824.1">
        <property type="nucleotide sequence ID" value="XM_019164288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NKR2"/>
    <dbReference type="GeneID" id="30180975"/>
    <dbReference type="OrthoDB" id="8907274at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF192">
        <property type="entry name" value="PHOSPHATIDIC ACID PHOSPHATASE TYPE 2_HALOPEROXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="110"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="353" mass="40215" checksum="65E432A5DC68A7F8" modified="2017-01-18" version="1">MIFKDTFYRVCNCQVSLFSFLSYLVDWLTYFIVLSAAVNWQLFAVTRYTDFSINDISIMHAYKAENETYAPVWYLLILILLIPLFIVVICCLWFLRNRNKKRMLWDMHAAILGSLGCCSSQLLLVVIIKNIAAVPRPDFLTRCQPDYSTIPVLGSLYNVDFCQNPDESLINDGFKSFPSGHSSTVFASQTFLVLFLIGKIKMSGSSYFSWKLVLSIMYPLIISCKVSFSRVSDHRHRVRDVLVGDLIGVCFGTLFYFIYFTNPFNGAPSLAFSPRKFEVDENSEGLFGLKISTYDIDSKEISYTESVDDGNNPAFPKSFSPLCYSTVPVKLKHTFNNRPNRPTSNPIGSLFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL44</accession>
    <name>A0A1E3NL44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_59079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017985.1">
        <property type="nucleotide sequence ID" value="XM_019163342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL44"/>
    <dbReference type="STRING" id="763406.A0A1E3NL44"/>
    <dbReference type="GeneID" id="30180029"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF4">
        <property type="entry name" value="PROTEIN KINASE MCK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="369" mass="42358" checksum="EA5848A975F64974" modified="2017-01-18" version="1">MTEYLVKDVTSNTSGITRPMFVQEITKIGEGAFGTVLQANLKNALSEPNSPQDVWIGPFAIKKVPAQTEYKSRELQILKQTNHPNIVALKYYYNYPNKDDNNRVYQHLAMECLPCNLQSEIKRYYMNKSFLPESHIRVYTFQIARAMNYLHSFQICHRDIKPSNILINPETLLLKICDFGSAKVLEPNQPSVSYICSRYYRAPELIVGCCIYTTKIDIWGLGCVMGEMYMGKPVFQGQEPMLQLREITKLLGPPSEKFIYTANPQYNGPMYSHKIYSSTAETRFQKVFNNADADAVDLLMQVLVYSPTERYGPSQIMAHKFFNEIKSINFTVQSRASTTPLKIDNLLFNFSDYELRTFGSLINDILPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL66</accession>
    <name>A0A1E3NL66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_77876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017974.1">
        <property type="nucleotide sequence ID" value="XM_019164958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL66"/>
    <dbReference type="STRING" id="763406.A0A1E3NL66"/>
    <dbReference type="GeneID" id="30181645"/>
    <dbReference type="OrthoDB" id="196165at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053039">
        <property type="entry name" value="Polarity_Bud-Selection_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47775">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47775:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="235"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="460"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="515"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="636"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="540"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="640"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="710"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="748" mass="82963" checksum="7FCADDB32FF293D5" modified="2017-01-18" version="1">MDTQQIARLSRRDLQSLHTSLDNLSDFNIESYGRDSFSSPSLGRSASQDGGDEDDELDDFALHNLKDLRLAPHGFSRPSVRDPDTQSNSSSSAASTVIHNPNSTPPAGADDLVNSVSLRTLESDPELQNKLHRTLRLSNTNNNNNNNKSLEPGFYHTGAQDACRGDEQASMDEIDYYQNEHDGDNGNEDDDDEEVDDDIGSSNSLDSMEDITNDKLTDYIQGMLPSPPSSPPKELDPTKLYALYDFSGPDPSHLPLLKNESVLLLNDSDSYWWLVRKVDNNRIGFAPAEILETYTERLARLNCWKNEILERGGIAGLKIEEEKKLFNTDYPLNHPLLESIPLVNSAMNNDSVDSVDNKIERKGSLKKPKHGFNYISTDSTKKTVSFADVNEVKESSDLENNDDEEDEEFDKESIDRIQERGEHAAACGLIDGIDENEETSYSLLEGYQNEYSIEESSIPVRSEGLPHEPRNGHDHTQRYNSKDDWDNADLAGTQPLIVPKKRRNFLIKNLDDYQYSSDEKETDARTQELEDAEEGVEQVKASSRSPEVLTPSSSANGNSLESTPTKLALNNVTASTCSDSTDSLQKGLEFKERKTQDLSVAKTRSTEKTAAAGETRLASLKMLDDLLDTYPEFVGHGKDSSTASASASNSGSSSMTNESGTKDVKAQGLAEGYRSSSMSSDIKMKSLLVPELDETETESEFSKSKGSLHMRSDSSIEGDDLHPKTMLIFDPLFNQIKELDALLDEIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL71</accession>
    <name>A0A1E3NL71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_33414</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017949.1">
        <property type="nucleotide sequence ID" value="XM_019162821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL71"/>
    <dbReference type="STRING" id="763406.A0A1E3NL71"/>
    <dbReference type="GeneID" id="30179508"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF50">
        <property type="entry name" value="HEXOSE TRANSPORTER HXT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="14"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="522" mass="57780" checksum="F1774623D6CE0228" modified="2017-01-18" version="1">MRELLSKHLIIGLLCLMVAFGGFVFGWDTGTISGFVQMPSFKQTFGEWNVKDNSFEFTNTRVGLIISIFNIGCAVGGITLGKLGDVFGRKIGLMGVMMIYIVGIIVQISSFHWVQFMVGRIISGLAVGAVSVLSPMFIAETSPAEIRGIMVSCYQLMITLGILLGYITTFGTVHSYDDARQWRIPLGLCFVWAVAMIGGMLFLPESARYLIEKGHMEKARLSVAKVNSMDPYDEVVQQEIETFTLAIAEQHEAGNASWMELLMGKPRILARLIIGIALHSFQQLTGNNYFFYYGTSIFKSVGLQDSFVTSIILGAVNFGSTFIALYSIGKVGRRPNLLYGSVCMAACLLTFSVIGSTILYPNGFDQPTDKQAGGAMIFLTCMFIFTFATTWAPGVFVVVSETYPLRIRSKGMAIATAANWLWGFMIAFFTPFIISHIRFAYGFVFFGCTVAAAIFVYLMVPETGGLSLEQVDVLYKYYKPGQAQNTRDEAEESVRVERLNALPPFPYPFEEKISMMTPNYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM01</accession>
    <name>A0A1E3NM01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavoprotein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15159</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018285.1">
        <property type="nucleotide sequence ID" value="XM_019160691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM01"/>
    <dbReference type="STRING" id="763406.A0A1E3NM01"/>
    <dbReference type="GeneID" id="30177378"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF17">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE SUBUNIT SIS2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="3">
        <location>
            <begin position="380"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="565"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="591"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="642"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="676" mass="72555" checksum="C18E00852DF29C99" modified="2017-01-18" version="1">MGETDRTTKDTGEVSGAPASSFLKNTVSAKMANSAGKDVESNTKATDEDLGSPKPLLKKDSLESPSVLAISNLRCPSPPPILQHARSLRNPSDGKGQSNEVGNNERDKNRDGGQDTGLNGKSAIYGNPVINVSSIGNSSDNNCSSVDSVIGGSQQCYQMMEENPSISKLLDTSLNYDGMAAAHAVTASMAAAVARDKFIQSDASTSGGNESDLKLSDSKPQFHQRTSSMHAHFYLDDTLKHPAASRSRSGSASNSDPLLKPAFDLPSNHLSNITVQAGAPLKDKKHSPEYRSNSNGSQLNGSIQSILAPGAVSTPSSSSAGNSNSGITYSTILGNLSQTASGPASVAMGSVSGTGTGTNSSAISPLSVFARLQHDDGKLHILFGACGSVSIKKTKLIINKLEEIYKEKVSIQLILTGSAEHFVSKGEFPANVLLWKDKDEWSTWNNRTDPVLHIELRKWADILIIAPLTANTLSKIALGLCDNLLTNVVRAWNSQYPILLAPAMVSFAYTSVITKRHVKVIRENMKWIEILKPTEKVMSSYGEIGMGGMMDWNEIVVTIVEKLGGYPDDEDEEDDDDDDDDDTRSGNNNKDDDDDDDDDDDDDDDDDDDDEDDEDDDEDGDHEDVQEQSGDTSNAGNAGKKMIKEGLEFKIEPKNNGPGERTTPILSTTTEETTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM28</accession>
    <name>A0A1E3NM28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16292</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017516.1">
        <property type="nucleotide sequence ID" value="XM_019161153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM28"/>
    <dbReference type="STRING" id="763406.A0A1E3NM28"/>
    <dbReference type="GeneID" id="30177840"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="51698" checksum="F5D4BE63BA6671A7" modified="2017-01-18" version="1">MSGVPKVAIIGAGPLGLGFAKAIAAEPNAKNIFGSIDVYERRPQAGGLWNHTGDKSKVTFALSESKANKNALKVTDNDPHLSPMYKHTETNIVKDMMRYKDYPLPANSNTFPNRSEVLNYLLDYQKTIPDFVNFKFNTKVQSLLKNTDNNKWELKTAESGETQNYDKVILAHGHFDTPFVPKAEGLLNWFNKDPTSVSHAKYYNDVEKFKDQKVLIIGNSASGIDISTQLLTVAKGIVMSCVGEPMFKGVIEDSTEQVDRVIKYDFENNRSVTTQSGKVISGIDKILFCTGYLYEVPFLKSYISGPKSLITDGSHLRYLYKQLFYIPDPSLILAGLPKSIIPFPFAEAQGQYVARILSGRLQLPSTEDMYADYEREVATRGNGSTFHIMGNLKDAQYCNDMYELIKGTENEGFVANFWDEQMMELRKKTGEIKKSRFNVLVKYAQGLREQGKPFVALKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM52</accession>
    <name>A0A1E3NM52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018332.1">
        <property type="nucleotide sequence ID" value="XM_019160713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM52"/>
    <dbReference type="STRING" id="763406.A0A1E3NM52"/>
    <dbReference type="GeneID" id="30177400"/>
    <dbReference type="OrthoDB" id="278430at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="2">
        <location>
            <begin position="171"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="755"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="795"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="814" mass="89206" checksum="53200DCDBFFF5B03" modified="2017-01-18" version="1">MAIKQSAGDMSQEDTEACKKAFEAEPDNGGGAETGASSRVGSAAEEQTGEAAVPLGTTAEASEGKTAVGEDAGQASETVVPEVPDVSEPPETQEDQVAPLNGVTGHLVEIPLDSGTTDLQNVEILSKHYQIPNILIKSLFDLNNKRKTQLLNGFNSAGSTTANSGTPGDVCRFILELEAEIIEFIVKPSVDSWKMKPLNSYYRLLSHQLADYYHLGHILSNDGSSMVLFKINTSLINADDETKKNAKFDQSGNIRPLDFRNLKFDPQEKLNRIKLADLYNAYKDTFYKGDYKSNTNTNTDNDGQAGITNEFSNLRMDTTDSDRAINNVRIMRRAANKTSSEGEEQRRIGNGSRTHSFVSEDSQLSKEERYKLVKERIEKEHAASDDYGDDREETVNGANGKNKINGEDAREDEDYDDDDDAEIIISDERGENGTTELHSTGGYRSDRGGYIHPGNSTNISKYRNRNQDTNGFRRNAKAYRGNRHSNYNYRNQYAYYPNGQIYAGLPPQHPPSSNSTTHHPPYGYMIAPNSSPSMPMNMDMSLATSPIGSPNSTSAMLPSPPYYYIPVMPPVSNGNGVSMNYKKNGSNKNRNSTEDVHASGAKEGNNSEFSQSPASVSNSTTSMANGEGFEEEISSPPLAASSHPVALQHTSPPPVPPGSSGASYSVTPPGMPVMMPMGPAQVSRPMYQYYYPLRGNQYYPPGANGISANGMYMPMMYYGDNMNKDGNYVMNRNKKYYNNGNGNVYYSGYNNYSRKSSKNYGRRNGSRSNGYDHTATNTDSNGSLSVDVDTGATKDTGDNYTDAHYESTVESVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM65</accession>
    <name>A0A1E3NM65_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018348.1">
        <property type="nucleotide sequence ID" value="XM_019160719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM65"/>
    <dbReference type="GeneID" id="30177406"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="351" mass="39579" checksum="872D58F15BAE159F" modified="2017-01-18" version="1">MENQKENEITIDPIIVNTNNNVTGTSAININKSASKNDFSMKRSMVFAGIPTTNIATNNNRNNTLVSGQYIFRKKAAPNVNQIDSKSETSSINQNINLSHPGLGMKNKFDMISTTNLLKTFNNQHPQENYSKNDHMAAIEPDNSMMQLASDPIEDYVTIFPHDANISELRNNNSNSYDLISSRLEKLDNLYDENNNARKDDDFKELDLAHIDRILNEDDSENERMANRHGKPLNDKKYNVDENNGFGGSCDGVDNGDIETVGERDGGEVVDDDEEDDDEDETENELLSLELGKVSLYLQDPNTHMKPADSEISINNSTNQGNYDLIDEINYALDDEYQIKHINLYSWDGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM88</accession>
    <name>A0A1E3NM88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coiled-coil domain-containing protein 47</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72532</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017576.1">
        <property type="nucleotide sequence ID" value="XM_019164225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM88"/>
    <dbReference type="STRING" id="763406.A0A1E3NM88"/>
    <dbReference type="GeneID" id="30180912"/>
    <dbReference type="OrthoDB" id="10039147at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="316"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="41422" checksum="B96C7F0C35C84EA6" modified="2017-01-18" version="1">MAYPQDITPEEFSKLPLLERLTTYNWKIEIFALGVVLAYFVLFKLGSGYNNKIASKFIDSVVPALRENFFQVGVTSQQLYAQDDGQHYSLYASGRLRVQSVLAKIELQSRQNLFMWAMELVMSFFLDSVPPPQDLVTVEFTFDEEASEKFDNFVWAVVTKDKMNTFRSENYFLSLTKTSESPKLPLQFVFMNEVSDMNDVLYTKKLADLLQANVSTLKFLAITDQPVEKPVKIAELKPYKRLILQFALSNSDSNIEASKNLFNYVLNDYLDLVVNRATFRPELTRKVKKTRENEYNKLKKILDDLKREELNTKKIEEQKKLKASMTPEEQQKFSKKQQERKQRKQLNRQKVRGSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NME4</accession>
    <name>A0A1E3NME4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018417.1">
        <property type="nucleotide sequence ID" value="XM_019163910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NME4"/>
    <dbReference type="GeneID" id="30180597"/>
    <dbReference type="OrthoDB" id="74460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053002">
        <property type="entry name" value="Metalloproteinase_M10B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021917">
        <property type="entry name" value="Unchr_Zn-peptidase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054:SF2">
        <property type="entry name" value="MIP04191P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054">
        <property type="entry name" value="ZINC METALLOPROTEINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12044">
        <property type="entry name" value="Metallopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="546"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="676" mass="75928" checksum="044FC4439A6EE398" modified="2017-01-18" version="1">MPITFENLPRDYEQLQVPVYILKGRSTAKSSGIIGAFSDKFSDQFYEVTAGYFKAYLHLSPGVNKITLKHLDGKFQPNGYAAFDTDKKPFDQVTISINYTPIPDKQFTVPKIHLCVLVGRDSPALFDCPANKMYEGNGLATAIKKLRVGGRLMQAFTLDEMNKNGCGKRCFRFVEEETTSTVSFQEEMNKVKRSEIKIHVIKSKRSVAEIRDANYAQQNQQAKEAGKLFEFALDALKDAGGVFADNATANVPALAAVMILDAHWDKPRNMLLGHAALGGGTDRIKLAIFGSQGLHSWPMNWESIHKSFTDCTALNTNEVVNDCNECSQYWECLCVSLGAFMHEIGHSLGCPHQENGVMLRDYVTMDRKFLTSEENCIRTKRGKWGPVLTKDEPGWNRLDILRFLYHPAFALPGDFEDTKFRPQFTLINQGMKLPNGLTGEPSFVAYDEDTLDILSDTGIYLVECHVGEWSRLHYEYIPSFYQGSDPQRYVRLKYSVIQQQLASEFKGKPVHLEVLTMGFGQRSIDNLQDYLKQHSKNITLSNNRSAKMSDKYGLRESKPIEAVFPDKAIAFFRISHGSALDGIETFFTDGSKVTFGNPKPHYSEFKLDPDEFVISVAFRSGAWVDSIQFTTNKRVSNNFGGDGGSLHKVPLPQGGKLLGFYGSLGSWIDQIGIYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMI1</accession>
    <name>A0A1E3NMI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="10">P5C dehydrogenase</shortName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
                <ecNumber evidence="9">1.2.1.88</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_72651</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="9"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017706.1">
        <property type="nucleotide sequence ID" value="XM_019164264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMI1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMI1"/>
    <dbReference type="GeneID" id="30180951"/>
    <dbReference type="OrthoDB" id="5322683at2759"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00374"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000006">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005931">
        <property type="entry name" value="P5CDH/ALDH4A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01236">
        <property type="entry name" value="D1pyr5carbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 9" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="11">
        <location>
            <begin position="103"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="593" mass="65141" checksum="9B9A66D585D93838" modified="2017-01-18" version="1">MLSRSVRTLRTSSKLISKASVSIPVVASVQFSRSAAQPTTSTRGVAQLGHFEAPRHITNEYTKLFTKDDVKDWELLKQELSNFTTNPQTVPLVIGGKRIESGRETKQIVNPANIKQSLGTYAQATKEDVKAAIESSLEAKKVWADMPFADRSAIFLKASELISTKYRHKMLAATMLGQGKNVFQGEIDCIGELIDFFRFNVKYASDLYRSQPPESATGVWNRTEYRPLEGFVYAITPFNFTAIAGGLVGAPALMGNTVVWKPSDSAILSNYLLLQILEEAGLPKGVVNFVPGNPVEVSDVVLNDTNFAALHFTGSTKVFSSIWSTIANNVGKGVYRDFPRIVGETGGKNFHLVDKSANVENAAYNTIRGAFEYQGQKCSATSRAYVSESVWPEFKKILTETVDSITPTNCTQELQGFMGPVIHEPSFDKVSGAIKTIAEDPELELLAGGKFDKSVGYFIKPTVVQTKNIEHPFMRNEFFGPLLTVYVYKDAEIDNVLEKIDTTTGYGLTGAVFSTDRLSIRKYEERLRYAAGNFYINDKSTGAVVGQQAFGGSRLSGTNDKAGSAALVSRFVSVRSIKENFGELKSHLNPSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMW3</accession>
    <name>A0A1E3NMW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15406</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018568.1">
        <property type="nucleotide sequence ID" value="XM_019160812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMW3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMW3"/>
    <dbReference type="GeneID" id="30177499"/>
    <dbReference type="OrthoDB" id="5229455at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF43">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="190"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="655"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="655"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="669"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="904" mass="99997" checksum="3328B9398F97BD3D" modified="2017-01-18" version="1">MKRPDSSESPAAEQGAGPQKRSRSRTGCRCCKKSKVKCDEARPVCGNCLRSGKSCDYSLKLTWGGRPYKVPRVEKLNPIASLSRSQLAAGAGGSGIAPAAAKAVKASCSPAPENTLQLPQPPRSSRQAEPAALKPPSLNPSENLSFLLKMSDPTLNIPGLLRKEPVSRTLRPPLQAAKPAPPAAAFQSLEESYTDTPVKKGPSPSTSEPSESEKVSSDIQIKKEPSSSTLEPSPLASEKVSGNIQIKKEPSPFSREPSPFASEKVSSNIQNLNTKLGSLFDEKLTQLSPWSFLEEIDKSVSCSFPDLPPELQYAGDPRVTESENAFSLNSQLLLNSEVNLMSNDFSSASDLMENLTNNFTKTQELPEQPLHTFFSVPMISGFRTPPRNHYDEETGLLAISPSPASTSSSISEIMEPIPRGLLPLPDMLLNAPYFYDSFLFFTNLTSSILTPADSSVYINNPFKKVLPKLAMTNNGLMSLIVAFGSAHKSLLLKKDSTKIVDSLLGRALSDLLVLLNNKETCTSDLTLTLVMFFSSFLAFNYKSDKWKVHMNGAKQIFLMRGYNRPFDKILSDYKTDGSLISGEIKKSKLLYFLIRWFAYLDIFTNLSSPLEPTEDEIKKYCANSPSYLDSLSPMSDSFSSPNSFSSSFSIMDANNNGRAPSNDIPQNNDDLDASNTDPDDQIDYEVAETEDGLMKDESHQDIDYMLGFKIKFLPIFSQLAKLIKRVNLIKRSNERMNIASKASSIGLSPSIIESAINIQTKFQKLKYTVYETTETKRAKSLNAVVASNHCFLLMGMIQLYRRVLLMPRNSKIIQEMCLGILDAMTKHIDTQQPNSLGLILPLFIAGCECQNVNLQPLFIKKLHDFQEKGSVSAESGIEIMAKCWRTGRDWNEIMMEYDRNVVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ2</accession>
    <name>A0A1E3NMZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actin patches distal protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018579.1">
        <property type="nucleotide sequence ID" value="XM_019163947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMZ2"/>
    <dbReference type="GeneID" id="30180634"/>
    <dbReference type="OrthoDB" id="10253744at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF14">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34574" checksum="5CED6B2419258FAD" modified="2017-01-18" version="1">MNYLKSLKSRVVGDNSESKIAEILPVSSEPFECKPNECVNGFKESCFSLRSDAAATPLYETAEPSSLHFLVSTGTTDWEHDAFEQKGTILNTFNSKANNLARSSNITIKSNVTNEPLNISDPQSITLNKLDVLILPWFVWIRGITKDNIDEVFTKIKQILQSEKNDDTVKGNQAENLISKLTNVEGLVVTKDPNMSVILLCSHRTRDKKCGITAPIMKKEFDSQLRDLELYRDSGDDRPGGVKVIFVNHVGGHKFAANVLIYNKHGEFVWFARCTPLNVKFILNETVQHHKVFPANVRACSTFNAVKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ7</accession>
    <name>A0A1E3NMZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_59001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017886.1">
        <property type="nucleotide sequence ID" value="XM_019163337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMZ7"/>
    <dbReference type="GeneID" id="30180024"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16023">
        <property type="entry name" value="GPI_EPT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037675">
        <property type="entry name" value="PIG-O_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="571"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="598"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="627"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="660"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="700"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="737"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="801"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="856"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="896"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="941"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="982"/>
            <end position="1001"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1016" mass="115446" checksum="95E57F1C676BCA71" modified="2017-01-18" version="1">MVSPSLRNTPISIPSSQRSDEIKDRYNYRAKKLGQTNSSYVKVMFLFGILQTIALAFFTRGFLLSRNVLPDLSTCETQQNGSCFHPSGFKKAVILVVDALRFDFTIPIPGVSEDSPDSNYHNHFNIFYDTFRKYPENSLLLKFIADPPTTTLQRLKGLTTGSLPTFIDAGSNFDGDTVTEDNLLSQFFHNNKTVAFAGDDTWTAMFSPFLNPNLTFPYDSLNVWDLHSVDNGVIDHVFPLLKKENQSEWDVLIGHFLGVDHCGHRFGPEHYAMKDKLLQLNDIIEKVMRQISDDTLLVVFGDHGMDKTGNHGGESDDEVEAALFMYSKKKHFKPFNHMNENVYDISKMGKNYRRVNQIDIAPTLAMLLGIPIPFNSLGSPIAEAFMADEKLYSVASMLTSSQINNYRLSSNELKGDENINELYRTLYQNWTLNSVLQPSLVDDSYKYQSLSLQRCKELWANFDDLNIWLGIFLIFVSLIVLIVYSKLVPSVVVSQLNYQCFVASIILILVYCVLCSSFSTVFKPEGLPLSWSLVLGVGLGIANGILAPIMDRYSIFFLILQVRENLVQNGWTYLGMLIIFLHSIIYASNSFIIWEDKIVSFWLSTFGFLAFFKSFQNEEKHKRFIGAYHSLIFMILTRLTSLITTCREEQGDKCVSTFKITWWSVALLYASTLVLPQIIKTFYKISYSYEGAAPMWISKGLRGTMLLIAILWTLEFVENDEFLNKNYDLQFIALKNWRIILGRIVLGVSMIGANFGWNVGPLCIQVELEQEEVETGMVKSKNKRNTAARSQQYEMRKTARIVGYSNVYGSSYFLFFINILCSILVVSKPLGGISLFFLVNQLLTLFELVDILNIRTNLISVVVICMLGYLHFFSTGHQATLQSIQWETGFMFTETIVFPVTHLAILLNTLGSFILVAIAIPLLTLWKIPPTNKPVALSSKIVENALTVLIYQTSLTLVTLIMTNNFRRHLMVWKIFAPRFMLNALILVLFNVVLTFVSVGYSSVKVVSRWYEVFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNC6</accession>
    <name>A0A1E3NNC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L30 ferredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_76661</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018718.1">
        <property type="nucleotide sequence ID" value="XM_019164951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNC6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNC6"/>
    <dbReference type="GeneID" id="30181638"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.15.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="6">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="82"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27257" checksum="A806E81FA0925C7A" modified="2017-01-18" version="1">MAAIPRPESQIKKAKRVERDSKAVAAAKKARKTVSKEKRAALYKRTEAYVQEYAAAERAVIDAKRAAKADGSYFVEGESKIVFVVRIKGIMKIPPKSRKVFQLLRLTQINSGVFVKVTKATTELLKLVEQYCAIGYPSLSTIRQLVYKRGYGKVNKQRIPLTDNAVVESVLGQYGIESVEDLIHEIYTVGPNFKQASNFLWPFKLSNPSGGWGVRRKFQHFIQGGSTGNREEYINQLVKKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNF6</accession>
    <name>A0A1E3NNF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Adenylate cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_32432</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018752.1">
        <property type="nucleotide sequence ID" value="XM_019162766.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NNF6"/>
    <dbReference type="GeneID" id="30179453"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046579">
        <property type="term" value="P:positive regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048580">
        <property type="entry name" value="CYAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21187">
        <property type="entry name" value="CYAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="716"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="5">
        <location>
            <begin position="1035"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1419" mass="160481" checksum="C0AFD0FF6DEF5954" modified="2017-01-18" version="1">MRPSSPASSEISKSNNTSRQYVTSKKSVKELKGSSRSSVRIFKGEKSSLLPCTLETTCKNILDVLRRKRFLKQDDDHMLVLKCGGLTRTLSLDEKPLRIQRTMLFLYGYTEKDNLDFIERTDLSFLFKFVVEEKGVELISEERKRMINPQNVNLDNWNLQDIPNFLYAEPMVILDVSQNPSLEFTKDFMHDSRNLTTLSFTRSGSPVFPLAIVYTPRLANLDLEVNYIKVIPPEISNLITLTTLNIACNRISQLPETFSLLTNLQYLNLSSNRLKQIPPQIFKIQGLKKLDLSYNSISEFPEEISALKDLEVLQIAGNRLSGDLPSFFEEFDQLIKIDLRFNKLSSIDSLKSAQKIEVIRATGNNISVFRSKAPTLFEVEMNMNPLTYVYFECDMTNLKVVDFSKGKLTSCTFTAKLINVEKLSLDYNHLSVLPDHIHNMKSLTFLSIFKNNLTNLPDSIKQLTKLRHLDLHLNNIGKLNENIWYLESLDVLNLASNLLDIFPEPPDKLMNTYNTPSLANSLKELCLCDNKLTDSSFPTLSLFQNLNCLNLSYNDIFEIPTGYVSHFSKLKSLYLSGNYLSSLPVEDLDELQNVETLNLNGNRFGTLPAELSKLTNLSSLDVGSNQLKYNIGNIPYDWNWSYNKMLKYLNFSGNKRLEIKPQHNHNDTGEKLDSFLGLKHLRMLGLMDVTITTDAVPDQSVGVRVRSTVSQLGKFGYGISDILGDSDSLTTRDVVIEKFRGNSNEFLITIYDGKNSVQHGGDKISKIIQETFEIHLAEELKTLSEDPNAENPKTVEDCMRGAFLTMNSEMSILINKDESSTFSSAAAHRTKTTDKLTLEEDGLSGCSATIIYIRDDYIFVANVGDIMGILTKSDGEFNIITTKHEPYAPKEYARIRDSGGYVTTDGYLDGVADVSRAVGFFKLIPHINAKPSIHKFKLTQNEEMIAIGTNEVWKRVSFELAADIIRQEKSNPIGAAEKLRDFAISYGAKGKKVTAVVLSLRQFTTKQKHHSGLKPEDSTLRKLDEEIEPPTGELAMVFTDIKNSTLLWDNYPVAMRSAIKVHNSIMRRQLRIIGGYEVKTEGDAFIVSFPTPTAALLWCFIVQSQLLTTNEWPAEILSSDQGFEIKDNEGNLIFRGLSVRMGLHWGTPVCEMDIVTKRMDYFGPMVNRASRVSSVADGGQITMSTDFYSEMEKVKKIHRLIKESKLDLGRAYGYKAKDKDVENQMNQLENIGMVLEHIGAKKLKGLETPENIWLIFPKPLSSRLKVLTSEGGEIQNKSNKLLVGGITVETAWTLRRIAMRLEKLLSRSNENQRAESTSHFYAFIDKVQKESEDAIYNKVSNMESFVLLFLDHTITRIENCVTVLGTRQILGEDKVLNCDTIHLLDLLKNMTGELSMLKARVDELEHDKKIVALDENPIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNK8</accession>
    <name>A0A1E3NNK8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15611</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018804.1">
        <property type="nucleotide sequence ID" value="XM_019160891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNK8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNK8"/>
    <dbReference type="GeneID" id="30177578"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF54">
        <property type="entry name" value="PROTEIN ESCARGOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="113"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="206" mass="24051" checksum="8C222E5B758ABBCE" modified="2017-01-18" version="1">MPEQLENFGVSAPHVAQQTKTIHQQRSELQMKVEEVFDEAAKTVKEDKKIEGDRRTRNDATALLSATENEDVIEKASLPTKKKYLCKKCMRSFDSSSNLTSHVRCHLEKNEQIRCQFCEKRFKRRNDLERHKYTYHFQFKLACSGIVDGRSWGCGMVYSRRDALVKHWNGRGKKCITSFQTLQQLPNNINLKDVRKKAMKNLTQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNS7</accession>
    <name>A0A1E3NNS7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018864.1">
        <property type="nucleotide sequence ID" value="XM_019160910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNS7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNS7"/>
    <dbReference type="GeneID" id="30177597"/>
    <dbReference type="OrthoDB" id="275457at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="61"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="406" mass="45756" checksum="0CD8CDAA03FA392C" modified="2017-01-18" version="1">MLSMNKSKLAFNATRSIAQINQSRNIYRFPLESDINITKTGKTKIAHGLGGRHSRTGYQVSVFGGTGFLGKILTSKLAKHGTITIAPFRNGRSKRHLKVCGDLGVVNFHEFDIRNLKSIEESVAHSDVVVNLLGSSTNTKNFSMADANIEGSNRIAKISKDFGVDRFVQVSSYNADPNSPSEFFATKGIAEEVVKQHYPDATIVRPAPMYGRNSKFLNDMLSIKVFGGNIIYRQEVYPTHVEQVAQALEKIVYDDSTAGKTYELYGNERYSRVELREMLKYMIHLNQYGFFPAASGYDMPAPEFLVKIWCLLNGKLNPSLEAFDYDTYRRSTLHQKIDPSALTYKDLGIVSDELADWLYKYVKPHIIASSQAKNRTVYGKEEVLKLRDYVNSPKNYLDLFGYGSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNX9</accession>
    <name>A0A1E3NNX9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_58165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018895.1">
        <property type="nucleotide sequence ID" value="XM_019163283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNX9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNX9"/>
    <dbReference type="GeneID" id="30179970"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="509"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="770" mass="90204" checksum="DE83908155E18466" modified="2017-01-18" version="1">MDSSINRKAPYVEYYKPEYSNMSQRPSQDLPQNLGPPPGNQFSLLPQYHQVQPSGQDPRFQVQRQEEMSQFRVLQPVLSQQQLYYNPSYQYGEAPYPTQYVSYRAPGQRASYSSSILRENTPEPAYNSPVSIPVSMHSGSSNSFGGLMQPQTQQLRRQQLQPLQPLQRPSQPQGHLEAPAADFETVRIPAELSSTESLQYPSMVRYLMQERRSFNVPSSYLQQQKQQQQQWQQWQNHQQQQQPQHQFQQWQPQSHHQQQHHILLHQPPSQPQHQQQLQPSHQNQPQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQPQHQLQPQHQPQPQHQPQHQLQHQHQHQHQHQHQHQPQHQHSHQYPHQPQHQHSYQYPHQSHHETQNHPQQENTQSQHHQLQRHQLQQQQAHTHVQMQGPGPVHMQVSQKSILERQDIPVQLQKQEVNLSGEQSPEKQDITHLSQKLEQVSNIARTAVPWGNEESKLLVQLRENGLTWKEIATRFSNRTLNACQFRWKRISLNGGIHSDENDLEQGNNKCDSKLEVRGEESKAKQVTPDDISTNKKNDNCINAANISTTGPTKRNAEINLFDESESSLSSEKEQKKERRSLNRSKRPLQEEAIEDRPYFKRLRSATRSSRGISNLLNSNVSETRIVNKAYFEPVKDKTKKEQDLFESKQRIATSKEDLEYDATEKSGVRTSNVQGESEKCTKKFSRVLKLEELVDNNVSRPLEVVKENTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP17</accession>
    <name>A0A1E3NP17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018944.1">
        <property type="nucleotide sequence ID" value="XM_019160935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP17"/>
    <dbReference type="STRING" id="763406.A0A1E3NP17"/>
    <dbReference type="GeneID" id="30177622"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="64"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="308" mass="33827" checksum="1856E63B95D8E46F" modified="2017-01-18" version="1">MATLSSVQTTLKLQSADKGKHVSSRGYDTEAKDGVNNIAMGIYKHPYRLPKFVDPYIKRDWLLNQMAGAFRVFAKKGFCEGPAGHISVRDPIDPQTFWINPLAVHFSLLTPQHMVHVDEQGNVIGGNRTTINAAGFKIHSTIHKTRPDVNAICHVHSKAGKAFAALGSHLDMLNQDACIFFKNHTLYNDFGGVVLESEEAKHIAQALGTTNRAIILKNHGLLTAGSNVGEAAYLLCLMDTCCDVQMQVDSVQASGRYRRDVIPDQEAKYTYDMTASAENLYSSFCADYEVEVASSNGALRSATSYHTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP98</accession>
    <name>A0A1E3NP98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dipeptidase</fullName>
            <ecNumber evidence="1">3.4.13.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:48940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:77460"/>
            <dbReference type="EC" id="3.4.13.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.19" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019037.1">
        <property type="nucleotide sequence ID" value="XM_019160971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP98"/>
    <dbReference type="STRING" id="763406.A0A1E3NP98"/>
    <dbReference type="GeneID" id="30177658"/>
    <dbReference type="OrthoDB" id="445695at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01301">
        <property type="entry name" value="rDP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF12">
        <property type="entry name" value="DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="MICROSOMAL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51365">
        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU341113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="432" mass="48523" checksum="996E4D3727A4C76D" modified="2017-01-18" version="1">MDAHTQIPPGTDIERRFEALSKRFPIVDTHNDFPYALRMQLHYELSSTPQFDFDSLLTSHTDLVRMREGRIGVQFFSCWIECKTDDILSQDFNQPSSIVRDTLEQIDVVRRLVDEHSGALRFVHTADEALACFQDQGSTQIAITLGVEGLHQVDLSLGVVRQYHALGVRYITLTHNCDNPFATAASSVTGGLSDRGLTVYGRECVREMNRLGMMVDLSHVSHKTMLDVLATTQAPVLFSHSSTYALTAHERNVRDDVLLLVKKNGGVVCINFFPEFLQQRGRARVTIDDAVAHIRHVVELIGWDHVGFGSDFDGIPQGPVGLEDVSKYPDLVKRVWAATGASEEQVAKMMGMNVLRVWKQCELVAARMRQTHGPVDSNWDQRRWVFHEYCKELPELFPGAFGLKQNEYTDTQTLVNGTAHDGSEDDAAHAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPA4</accession>
    <name>A0A1E3NPA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Saccharopine dehydrogenase [NAD(+), L-lysine-forming]</fullName>
            <shortName evidence="13">SDH</shortName>
            <ecNumber evidence="4 13">1.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">Lysine--2-oxoglutarate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_10847</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-saccharopine + NAD(+) + H2O = L-lysine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="EC" id="1.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AlaDH/PNT family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.7" evidence="4 13"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019015.1">
        <property type="nucleotide sequence ID" value="XM_019159721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPA4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPA4"/>
    <dbReference type="GeneID" id="30176408"/>
    <dbReference type="OrthoDB" id="265306at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00034"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004754">
        <property type="term" value="F:saccharopine dehydrogenase (NAD+, L-lysine-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12188">
        <property type="entry name" value="SDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000217">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000423">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027281">
        <property type="entry name" value="Lys1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF23">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE [NAD(+), L-LYSINE-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018250">
        <property type="entry name" value="Saccharopine_DH_Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 13 15" id="KW-0520">NAD</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="18">
        <location>
            <begin position="9"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="17">
        <location>
            <begin position="177"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="320"/>
            <end position="323"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="207"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR018250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ47902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="374" mass="41887" checksum="D3D5A629160D5910" modified="2017-01-18" version="1">MAGNKVILHLRAETKPREARAALTPKTVKQLLDTGDFEIYVEEAPQSTFKTEEYRQAGAKIVPFESWKTAPRNRTIIGLKELPEELFPLVHDHIQFAHCYKDQGGWEDVLSRFPYGGGTLYDLEFLENDQGRRVAAFGYYAGFAGAALGVLDWSFKQLNSDSKNLPGVTPYPNEDLLVKDVKAQLKKAYAKTGRYPTVLVIGALGRCGSGAIDLCKKVGIPESNLLKWDMNETKKGGPFKEIASSDILINCIYLSKPIPPFINYDLLNKDDRKLRTIVDVSADTTNPHNPIPVYTIATQFDDPTVLVETTKGPKLSVISIDHLPSLLPREASEFFARDLLPSLKELPNKDKALVWVKAKKLFDHHVKRLNKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPD3</accession>
    <name>A0A1E3NPD3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc finger CHCC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019084.1">
        <property type="nucleotide sequence ID" value="XM_019160988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPD3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPD3"/>
    <dbReference type="GeneID" id="30177675"/>
    <dbReference type="OrthoDB" id="307899at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.40">
        <property type="entry name" value="q5lls5 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019401">
        <property type="entry name" value="Znf_CHCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-A SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10276">
        <property type="entry name" value="zf-CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger CHCC-type" evidence="2">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="158" mass="17265" checksum="8AE32686BBE62BCD" modified="2017-01-18" version="1">MLARTVRINSVAARRFVATASKSSTATKTQEKEAGAATGSSTALTGEDPLDVIPSSNYPQAPNRAETWSQSQKSRDEIYKTDPRFVGKDLSKQPQSLAAIELIKQQPVSFIHGNIAVCHGTDFVQGHPKVYINLDKDKAHSCGYCGARYAREELKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPI6</accession>
    <name>A0A1E3NPI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome activator complex subunit 4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_57926</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018576.1">
        <property type="nucleotide sequence ID" value="XM_019163262.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NPI6"/>
    <dbReference type="GeneID" id="30179949"/>
    <dbReference type="OrthoDB" id="17907at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032372">
        <property type="entry name" value="Blm10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16547">
        <property type="entry name" value="BLM10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 N-terminal" evidence="8">
        <location>
            <begin position="43"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="7">
        <location>
            <begin position="481"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="6">
        <location>
            <begin position="1910"/>
            <end position="1995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1996" mass="227786" checksum="FDC7E6C9DA13FFE1" modified="2017-01-18" version="1">MSSPVPEFNFLKPSSRRPINVGKNCNADVDQARSFTSSHTIKELNRLQAYGLDIPNDDVEAERLLHDENSPFFATDKIRKLKNKLVLPYETETIRDQYKYLSHIISHIYIAVKSLDLKSNLTISVEDLEAAKEVIINNRDSDSYIRSAVGSVGDGQKFETLHHNESEAIEDDEDEDDMSDYYDSSDEEDEDAANSTPVMKVEPESASIISLKYWTKELKNLLKMGLSIPLSIAQKLIKVFYAICLSRGQNVDIIFYTQVLTLLTREKKLLIRSGFRLEWEPVYAEFSTTLGNPTTFGPSMKDSRFKKLISFALTVKPFFPDGCIPALMEKIVSKLTVQTVSPSLIHLTVMLPMQFKKPIINPDGSVEYDETDVRHYLPVLFDLWINHKSDKEINCLLSIIMNIAVENLKQGAANNTILHRGVYGIFTKHQYKYIMNQLFLSSQIRHKDNKMVKYCRTLSQLIINSLSAQHAFENEGVVDFLKTYVDAIFTMIHPSNSGPWSAVLSEVVKKLAVSLHFRVQGEKECRDLVPLYHPNYSALPDTVKLDDKIIECVVKTLLPLIRLGSQSKSAAQRRHYNEALQALCYIKPSLVLDSLLPDWYSSFESVNSTHRIPIVINQLAQLARYMVQLPVYRVHLPRFLSMLIPAIDSNDPEKTILTTDFITIVAAVIPFADLTEGSGDGGLCAIDFTSQHLSYLEAKFYELAPASSQLGEFEIPDKFKYDSEFELAALESASSSFKEFLMQFCDNCFKFLELAPAIDNTSTIESQACALISYCFEALIESMSDDLFDAVADKFFQYVSNNVRHEVALVFCNISEAIVRRNPKTQLKRIMDFFMPHVITEIENGAGLSRSQEVLGKDQRLIWNLRILAGAISGAGTEILPYLDDLEKFLVQHTYQLRGTAGLCAAMIANCAFSTISYLKPLERRLISQTWLDEHGGKYSAECWGGFQFSDIRFDPKYLDFKWYKPGSTEVDAVLPTRAPTPTLTDSIEAVDSTLTKRSDILYTYGPHFSNNVLAKTLNPSYQKLHHTRNLIGNCLHELVKALVKKDGSIELIGKVIQCISTWLNDCGYYSSDNELYTDNLHFISVLDLPGVFAPYTRTILGSRLAVYHCSRINISCCTRLPTKLDKLLIRDLVSLAASPYGITASHSASVLGMSLNRVMNCTSVVFNIFKDWEHALLNKDKEILLNIMIMFDKKKLRGLVEKSSSMLLKYEDLLFRSAELNEEEVTVMTMRLFKSIKKYVKIPAAVCLVDEKLLESIRPPDPDVDFKIETLKLAKSKKRAMLLSLLSKLVHTSLSRLSKKLNWKLMLLVMELITTIQSHIEIPLESDVLLTLVKFVDGSHPEISKKGMFWIASIMDTVQSRAFSKYDIGDLLSLKPSDPTIGPLKDILPDNCSQSFFHEIRNIENPLFFIDTKEWIPTSPWNKELEVVKPYESSSFGYNDSDERSIKQFASKINKKWLQMLLQTHIDESEVTCAFFPGIVYFFSSITSLIISGYVEDFTLTDLFEITDKIYKGDERSTHVVLGEVFSGILFACKTNHVAMKFADKEIANRLKSIFDNDLSQSTYKMWSIFSWWLSSHFDMRRTPEILDLICDFEVDKNVKRSPFGLICRLSFLKSYLGSEMNRFHNFEEIAHKMFTILAHPYDSVSKEVSAILFDVLYYESTDCIDTFDQFMAEVKKDQTGSGHIEHKKNVLFIEHLKLFMSKTLELAKENEGKTPQEIVNSDFMFHIKGLSTLLLRILKTSMNTTIVDFICPYILPLALELDKVKDACKLAEISVDALFLILGSIRFSDAQNEEIINLLETNLGWNTPKLTQYKHILAFFGVYGTVRFLERTSGQRKKLMEITSSMLFNQHLYLREQYSYNIKLFIHLFLDSEREEVINFYIKKFKKIIRKNRQEKDIKLTVEQTNLVHGATLGLCALVEAYPYTTPPPKWLPEVLTILEVKCTGYSGIVSRAAKDTLSQFKKTRQDTWHIDSKFFTEEQLEDLEGVLYKSYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPJ8</accession>
    <name>A0A1E3NPJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_31217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019147.1">
        <property type="nucleotide sequence ID" value="XM_019162683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPJ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NPJ8"/>
    <dbReference type="GeneID" id="30179370"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107">
        <property type="entry name" value="NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107:SF7">
        <property type="entry name" value="RE15974P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="249" mass="27175" checksum="58CBE17CEC37E223" modified="2017-01-18" version="1">MFSGKATIITGANKNLGKQTAEAFAKEGSDLLLHYHSSSSAAETKKLADELAKKYGVTTATYQGDLSTEDATSGLFDLGIKSFGKVDFAINNVGMVLRKSLADISLDEYNKMISVNVTASFLFLREAERKLAQDGSVVMLTTSLLGAYTDGYSVYQAAKSAVYWLTKTASKDTKKKITYNCISPGPMDTPFLYGQENDQRIEFFKTQAVNGRLTEVQDIVPIIKFIVKDGKWMTGQNLYANNGFTAPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPK9</accession>
    <name>A0A1E3NPK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MFS-type drug efflux transporter P55</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_23268</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019140.1">
        <property type="nucleotide sequence ID" value="XM_019162303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPK9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPK9"/>
    <dbReference type="GeneID" id="30178990"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133453" description="MFS-type drug efflux transporter P55" evidence="8">
        <location>
            <begin position="20"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="4"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="84" mass="9083" checksum="DE7DAD2B1D075FAF" modified="2017-01-18" version="1" fragment="single">LVSIIGSLWLGTFLSAADTTIVSTTANTIASSMNGSDKLTWIATSYLLTNTIFQPLVGRFSDVFGRRTVLLIAQFWFALGCLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ31</accession>
    <name>A0A1E3NQ31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthetase</fullName>
            <ecNumber evidence="7">6.3.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15577</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>7,8-dihydropteroate + L-glutamate + ATP = 7,8-dihydrofolate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="7"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018766.1">
        <property type="nucleotide sequence ID" value="XM_019160877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ31"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ31"/>
    <dbReference type="GeneID" id="30177564"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="445" mass="49444" checksum="2E42EFB1E43C7AAC" modified="2017-01-18" version="1">MLTGFPGTTSYPECFLKFPDVCRLYKLTHGIAMPLDLQLHRVKKLLTLLGNPQYKPQFIHIAGTNGKGSVCSYLSHILSASGIRNGRFNSPHLITPRDSIQIDNVPASLSLYEKCKEIIADTNAKHKVGCTEFELLTCTAFQIFAEAKVSIAILEVGVGGRLDATNVLAPDRTLVVGITKVGLDHQNLLGKTLSEIAFQKVGIFKKGVPAVVDGSNVESVLKVAEAESKDVGCPLYVVKREEDCWVTPKLLGDYQFDNLSVALKIASLLDDDRITRDTIKKGVESTVWAGRLQEYSFNMRSLGFEEDRPLDILLDGAHNTQASIELGKYLKTLRNDNDFIFIIAITQGKNLSALFKPIITFKDSIIFTQFDDKVEGMPWIKPFDVLDLKKEIETQKIEHADVYTCPQLKDAIFQAYKLSQVTKKKVVVCGSLYLVADVLRLQLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ98</accession>
    <name>A0A1E3NQ98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_124916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019329.1">
        <property type="nucleotide sequence ID" value="XM_019159933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ98"/>
    <dbReference type="GeneID" id="30176620"/>
    <dbReference type="OrthoDB" id="3986846at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="472" mass="51862" checksum="3EC7E9C885D80AC4" modified="2017-01-18" version="1">MDSKYGSHYPQQQQSQQQQPFQVHPSQQYKSHNLIARGRQNQIPKRYEFQKNIPQRTSSQHNQQLLLSRIRVEEAKSPRSPLFPGPTAREGSIGSQGSQGSRLGFSTLQRVKSTGPGASSLAPTANLAQFKRHSIADVGNLARSSSANVVSGKHGLNVYNDSTVFETTVAGCRNYAFERQMLQQRQYEYVDEAMQQRRRIASVDISNGGRYNRQPAQAAFVPHDPAGHHTPHFFRSPRFNGRSTDNVANPAGVQGWENGEDPHSGRNSIFEDPKVDNTVASDDDVSTVDMDGLDEPFADDGKSDVTLHSATLHEVPYDADEPFLPTRTQQRKLAIKHELDGAADTDNLPIGQEWMDPANYTGKVHSHLSSASSASTAAPSSASRGNKYAPAHWMNSSLKQRNMYEHVTSELRKMVLFNEKPLLESVARNRKVQASRGGAIPSGGATGAPSREDVGALVAGMWQEARGRALVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQA0</accession>
    <name>A0A1E3NQA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019345.1">
        <property type="nucleotide sequence ID" value="XM_019161069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQA0"/>
    <dbReference type="GeneID" id="30177756"/>
    <dbReference type="OrthoDB" id="10266265at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="66764" checksum="D9B959A78BBC16BC" modified="2017-01-18" version="1">MDLYKVAQNYIEKMLAVDADSDNKNIRVLLLDESTASIISLISTQSDLLKKEIYLVDRLENENRDKLRNLKCICFVKPSDLTVTKLSQEINNPKYSSYEIYFNNIIFKSRLERLAESDDLEIVNKVVEVFQDYYVLNKSLFTTTNIMSPFTASGIQTWDPLSLESSTECLTSLILSLNMKPVIKYESNSKMASKLANAFNYEMTSNSQLFEQLTPKRDTAPLVLIMDRKNDPVTPLLFPWTYQSMINELLGINNSNNSVNLSYLPNVGEELRTVIMNETQDPFYARSMYMNFGDLSTLLKQYVDDYKIKTKTNSNISSIKDMKFFLENYPEFKKMSLNLSKHMLLTSEIDKQINTQRVWETSEFEQSMCCNADTSHHENDVSELESFLFDSTRSDGTLANPLSEEIKLKLLSLYALKYETYPSNHLNSLLKRLNNPNISNFINILLKYAGQKCRITGEDGAVFNKLGGTVGAANQVAALFSNISGGNNTENAFMQHQPRIANVLDKFIKGKQSLSNTFVSVGKDNTDSKIPFREIVVFVIGGVCYEEVRIIEELNKNHDGVRIVLGGTQTLNSKIFIDNLREAGSTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQH7</accession>
    <name>A0A1E3NQH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71825</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018906.1">
        <property type="nucleotide sequence ID" value="XM_019164018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQH7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQH7"/>
    <dbReference type="GeneID" id="30180705"/>
    <dbReference type="OrthoDB" id="10254721at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="601" mass="67602" checksum="10510D2BAF75F696" modified="2017-01-18" version="1">MARSYHTLESLAKTSPYTALMRPDEDVPTPDKVSIAASKTPRQLKSGFFTYTYPTERKAYEFLSSSTRAMLDLGLDPNAEPKSQFFRDIVSGKTTYVSEKDGVHPFAMAYAGFQFGNFAGQLGDGRVVNLFTVKNPETGKSYELQLKGAGKTPFSRFADGNAVLRSSIREYVISESLNAIGIPSTRALAITALPENRAQRSGAEICAVVCRMSPTWLRIGHFDYCRMKGDRKGLLELCDYINGEVLDGKKYTKELEDFIAGDEKLKQLELTDYDKLFLDIVIRNAKSVAYWHTYGFLNGVLNTDNTSVLGLAIDFGPFAIMDKFDPNYTSNSEDHTLRYSFKNTPSAIWFNMVKLAESLAEILGATPELLKDENFQKNGFPDEKSIAAATARVNSLVQAAGDVYEKVFIEDYLRLVCGRLGITPKESDNSEILGLLFETLQVTKLEYNKFFTILQSLPLRDDDFDAGLAALAFLPESLQAPEQKEERDKVLKELRVFLTIFRARVEEEFLTDEVRLERARGSNPLFVPKNWVLDDVISYTTEKLRAKAGEAEAGAYLNKIMKMANNPYDRSRWGEELKDVEERWMSDVDDSKMMLSCSCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQJ5</accession>
    <name>A0A1E3NQJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_13959</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019490.1">
        <property type="nucleotide sequence ID" value="XM_019160153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQJ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NQJ5"/>
    <dbReference type="GeneID" id="30176840"/>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="169"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="293" mass="32655" checksum="2262AC5B57B90FD8" modified="2017-01-18" version="1">MELPTTERSGSQQGKLAKGQTRSELTPEQIQQQNILRLNGFSSSTILKNASVNNSATGSPILSGAGYSDIGLASASRIVPNGNNGFNAEPNERSTDGINNGDIPLNSISNPDEEIDDDALEDMDKFGLKGLISLLRHEDNDQNNIAKGIDLNMLGLNLSLDNKSHILSKTLSSPWLETSRSEVEPLFTKPDSFTIKPNDLLPIEDRISSFNDLTLFFIFYTKPRDILQELSARELNRRNWRYHKELQVWLTKDTNSEPKAAGIGAEEGTYIFFDPVSWEFVTKTLVLYYNSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQL9</accession>
    <name>A0A1E3NQL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14 17">Homoserine dehydrogenase</fullName>
            <shortName evidence="14">HDH</shortName>
            <ecNumber evidence="5 14 17">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_70010</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of L-aspartate-beta-semialdehyde (L-Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine; production of homoserine leads to a branch-point in the pathway as it can either be O-phosphorylated for processing to threonine, or O-acylated for processing to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-homoserine + NADP(+) = L-aspartate 4-semialdehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:15763"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14 18">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="5 14 17"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019478.1">
        <property type="nucleotide sequence ID" value="XM_019163530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQL9"/>
    <dbReference type="STRING" id="763406.A0A1E3NQL9"/>
    <dbReference type="GeneID" id="30180217"/>
    <dbReference type="OrthoDB" id="67851at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000006">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011147">
        <property type="entry name" value="Bifunc_Aspkin/hSer_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070:SF5">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 14 17" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="9 14 16" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 17" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="20">
        <location>
            <begin position="10"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="19">
        <location>
            <begin position="151"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR036497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ48365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="361" mass="38819" checksum="8CC60CAA9E9B2744" modified="2017-01-18" version="1">MTAVNIAIIGAGVVGKAFIEQLAGIKSKTISYNLIYLATSKKALISKDYSPISIFESLDLLSQSTAAPYQTPELVNYLAESSLPVILVDNTSNEKLAESYPLFVENGISIATPNKKAFSSNFKLWNDIFSAAGAANNGLVYHEASVGAGLPLISPLKEMVETGDEIVKIEGIFSGTLSYIFNEFSTIDPNTTKFSEIVSVAKQLGYTEPDPRDDLNGLDVARKVTILARISGLPIENSTSFPIHSLIPKELESVQSADEYMSKLPNFDGDMDALKAEAAKENKVLRFIGSIDVPNKKVSVEIAKFDYSHPFASLKGSDNVISIKSKRYTNPLIIQGAGAGADVTAMGVLGDVIKIAQRIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQQ8</accession>
    <name>A0A1E3NQQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_31859</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019141.1">
        <property type="nucleotide sequence ID" value="XM_019162727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQQ8"/>
    <dbReference type="GeneID" id="30179414"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="46543" checksum="C71E2C3ADF988BEC" modified="2017-01-18" version="1">MRGIYQGYANLMYAVGQFTGPILGSAFLAWNETSGWRWMFGIQVPMVIIAGVLVRRNVHEFRQDQLLEIDPKDRFAWKNLSQIDLPGSFLLSCGIISMLMMFNVTSVKSLQICIVCFVLSATAFALVEKYVMTVHIIPPSAFKGVLRITALVIFFGAAAIYGMNYVLPLYFQIVHDFSQFQLGLFNSFGVFAVSFGSLIAGWYLKHDDENIGPNVIISRGIHLSIVCCMGIVTGVFLCMFAALNLKPTPDRSDLTPKVYAKLSILLLGSIISSIGYGAFLVALLILVVGLVGIKRQATVTGMNYMFRSMGQSSGVGITLGLYNHNLFNELHKYFSKKSKPDGSQILERLLSSTAFIRNGLPTEYLHKVLKIYREAVGDAMILVFLLGAAAFLLSLLLKLHSTKEEPNRREPIPSSSSSSSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQU8</accession>
    <name>A0A1E3NQU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_8977</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019548.1">
        <property type="nucleotide sequence ID" value="XM_019165052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQU8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQU8"/>
    <dbReference type="GeneID" id="30181739"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="425"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="728" mass="82527" checksum="9D0C01EB925F852A" modified="2017-01-18" version="1">MISTLDVIQESFEDVPLEYAPTPPNHFYGLNRQNSMRNHFSSANNVAGHASASALSLNSNNSNSTVNMNLNGGRNARANYSASSLNNSSRELLLTPSQKLKIIKKQRVQMSQDQLSTNCPEALNAIDFLSDDELPDDLIVYDVPSLYSLQSLSHNKPRTRRPSVRRNNSISSHSDNNITNTHNINQQIENTTPSSNSNSNVNTTSSSYDSNNTNNLTAATKYHYQPPHSHFVTSSCSSSASSATSSPATRASSIFSCASDISEFSCADDEYLNPLSADARLLLENKDYKLEEALQRMSMLRNMSHLCTKNSLTANNTGNRNSDPNVSLASTLSVKSEPRSQYLSSTRPVNLPPKTKYEVSKHEKDYQNILEAEIYNEKEKLRDYQVKKKLLIAQAAKDEKLWSKVINNYDVLIKLPQTRELWWRNLPDKYRGNIWKKQLISKKNVVFGNSVLKKALSDASSTLNQACNFKSTKDELAKRQKGKVNPDLLEDVRFIEECADKLQYSFPEMKYFQYGENFDAVLQILIAIRALKNENKNLESIDIMKTINLVCILTYVFDDHLTTLSCFISLLSKKLPNFMLCSDEIQIPSDLLKDLEIQKSKTQSTYFGDIKDQFDKYLLQLTPRLYNHFIQRDINSLKIIQGLACSIFSNQLPFDVVLRVMDIYFFEGDILLLRASLALLKKISFKLYGSKEEVYNLLKVDGLINSSYLEVGEVDEFIKDIRDVLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR43</accession>
    <name>A0A1E3NR43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_26067</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019657.1">
        <property type="nucleotide sequence ID" value="XM_019162429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR43"/>
    <dbReference type="STRING" id="763406.A0A1E3NR43"/>
    <dbReference type="GeneID" id="30179116"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000006">
        <property type="entry name" value="Putative DNA helicase INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001269">
        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="20">
        <location>
            <begin position="237"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="491"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1050"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="167"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="306"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ48544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1221" mass="141003" checksum="D84D86426FCED3D9" modified="2017-01-18" version="1" fragment="single">ELLSVFQQKLNSAVRLDNLANTSINHLKFLNDEFQFLATTNGVCLDNKFAENLKNDVNLLNSKNSILEKIFKRHKQVESESNLLNNLKSEVLNYLVDVQTEREGKKTSLASTAISNNAAAGSKRRRKPTTKAEEMLDEADMENEFAEIFEQPKKRRRRAGAAAAATTAAASSASTSKSSAATATAGRSGATKKSPDADENTLESNDENDDQEGTTPKLSVKELKSLERQYDNTYLSIWKDLSRKDSPKAYRLFQQTAQAKSINLRKTAQLAARESRKWQVKTTKAQKDQVTKARRSMREMLNFWKKNEREERNLRLKAEQEALQRAKKEEEEREAKRQARKLNFLINQTELYSHFIGRKIKTSEFEGDMGAEPIPGYGAGSASHKVANEADVDMSLATKDFDNLNFDEENEEALHKMAAANAQSALMEAKNKAHQFNDGIPRASDGDDDEKNEGEMNFQNPTSIGDITVEQPKLLNCTLKEYQRKGLNWLANLYEQGINGILADEMGLGKTVQSISVLAYLAETHDIWGPFLVVTPASTLHNWQQEISNFVPNFNVLPYWGTAKDRKVLRKFWDRKNIIYNRNSPFHVVVTSYQLVVADSAYFQKMKWQYMILDEAQAIKSSQSSRWRSLLSFQCRNRLLLTGTPIQNNMQELWALLHFIMPSLFDSHDEFSEWFSKDIEAHAQSNSKLNEEQLRRLHAILKPFMLRRIKKNVQSELGEKIEIDVYCDLTNRQKKLYRMLRSQINLMDLIENNKRIGTNEDVESLMNVVMQFRKVCNHPDLFERADTKSAFCFGDFPMTESLIREVNESTLELNYNTKNLIKYKLPKTILEECLLPSYEKRSLTEKDSLINRMFNVYQPRHYIHDDDSELSILKFVNETPCEIEKIVSRNLVTNAINNKSYIESTKDEQNRLYREIYCTDNKNSLIFSDSKDRVPSFLKNLTHDVSKSMYMRSFQPGYFETALASPIEIVCSKENFNVIKDDAFFNKTIRQALLPLDLNTQAELLEKQVWVKDWPKAEMLPVEDSNYKQGGTIRLPSMERFVLESAKLKKLDQMLVDLKKGDHKCLIYFQMTKMMDLMEEYLSYRQYKYIRLDGSSKLSDRRDLVNDWQSNPELFIFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLLVRGTIEERMRDRAKQKEHVQQVVMEGKVDAVNEKEEKEREKKEMALWLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR48</accession>
    <name>A0A1E3NR48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cAMP-independent regulatory protein pac2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_122567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019146.1">
        <property type="nucleotide sequence ID" value="XM_019159904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR48"/>
    <dbReference type="GeneID" id="30176591"/>
    <dbReference type="OrthoDB" id="5572844at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF1">
        <property type="entry name" value="CAMP INDEPENDENT REGULATORY PROTEIN (AFU_ORTHOLOGUE AFUA_3G09640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26388" checksum="0CD8C1D342B0AB3D" modified="2017-01-18" version="1">MESYNGILCTKRDALFLIEAAKQRLVPLVTQRFSSYERQTSIRAGAVFVWREQGSNIRRWTDGRKWSASRVSGVFLSYREMQPPRSQTSCHVVDEKHGGLIKQSFSYCAGSGDKFHVVGYAESDLAADPARLARCQRPSLDPRFRALAAAVDAAAADPAEPPRPTTAAGTGRKRALSVDSASPAKRLSTSPPPSPPSPASPLSPLRSQPPCPVLPPLQSLLGPAALGPCDFDRKTLLALRCSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR95</accession>
    <name>A0A1E3NR95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019704.1">
        <property type="nucleotide sequence ID" value="XM_019160239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR95"/>
    <dbReference type="GeneID" id="30176926"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="64" mass="7160" checksum="98D5CA6C8CFC385B" modified="2017-01-18" version="1">MQYHANASHIVWLSGKHVDKQRRPASSPKKANSGSITLTSWPEREPSSKHKNLLLTGLQKLSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV1</accession>
    <name>A0A1E3NRV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_28610</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ATX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019877.1">
        <property type="nucleotide sequence ID" value="XM_019162548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV1"/>
    <dbReference type="GeneID" id="30179235"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000008">
        <property type="entry name" value="Copper transport protein ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051881">
        <property type="entry name" value="Copper_transport_ATOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365:SF1">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="70" mass="7799" checksum="36C61C9385719EBA" modified="2017-01-18" version="1">MHYQYNVKMSCSGCSNAVSKAVNRLEDIDKVETDLKTQTVDVYTAKVDYETVLEKIRKTGKEVLGGKVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS70</accession>
    <name>A0A1E3NS70_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020078.1">
        <property type="nucleotide sequence ID" value="XM_019163672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS70"/>
    <dbReference type="GeneID" id="30180359"/>
    <dbReference type="OrthoDB" id="3997567at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="119" mass="14519" checksum="48491482E8D65C74" modified="2017-01-18" version="1">MDNRVKTVIEREEERRRVNVELRERYRRVLRRTVLSFDSPGRERVVYENEKMIDRVQEELDGRVRYMEQVAKALQDRVEHTEERMARSETDKVQQRVELLNQKIRILEATVRHIVSRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSG4</accession>
    <name>A0A1E3NSG4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14461</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020059.1">
        <property type="nucleotide sequence ID" value="XM_019160384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSG4"/>
    <dbReference type="GeneID" id="30177071"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="63"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="133"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="133"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="136"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="186"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="193"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="283"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="447"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="493"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="530"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="563"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="741" mass="83630" checksum="B2EEFFBBAEAFDC77" modified="2017-01-18" version="1">MADFENLKEVGTEATQKPNASANQNNGDNSNGKKSRPALVVEKPVVGSIDHLAISKSLGYQIFRKTRRNAWNADEDKCLKELLIQQHTKNNRLSEYNDEQIDIKMIDWFDIASKMKTKRKGKECKKRWVSSLDPFLRKGKWTKSEDEALINAFKKHGASWQKVASEIKGRNEDQCSKRYTEVLNSDTKERLKPWSLEEDLILIDQVKQLGTKWRQISGSLPSRPSLTCRNRWRKIMTDIAKDSASNVIKRAVGVLDENGVPTIKFKQTNQYEEENIGKINKIFQQERSKSDSKAQGDDNSEIDAEDNSTEKSKRILEDDHDTPGSSKRQKSGNNSTAKKAPVSENYNTTTSYPRIETPVRTQTDWSFSLLDPRTNEELKSYTGDINTQELAHYLIELARFNGVTLTVHQHIHHHYSSSAAAVSSDPQTNISRYGHFNYLPPLIEVPKLTSSSSPDNTAESSTSAKNYTENPLLRLLNNDDGSNNTSNGFDSNRPINSESSESGTNLPNKKASLSIGDSKSNFLKSHRQQLHASRPSQYSEQSHNLEYSNSNSNLQSPGPTDTRGTKTENIKDGEHTGKSYTTEDLGEELDFWETMRSISRPKNNKPVSQHHPLHYYQPSNYKEQLPYQPSDSFGIQRFPNMATPLVTNMIRGEKNTKSVETSVTNVNTDESYGGMYMEGENDDAEEEEEMDIANQYGLYYNVFANKSQANYGSYGKQQNNGVNSGAVDLVNSGYLMPFNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSR9</accession>
    <name>A0A1E3NSR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_28763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020298.1">
        <property type="nucleotide sequence ID" value="XM_019162555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSR9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSR9"/>
    <dbReference type="GeneID" id="30179242"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990738">
        <property type="term" value="F:pseudouridine 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="245" mass="27650" checksum="2B2C3122C06FE6C4" modified="2017-01-18" version="1">MVHPVKFKACLFDMDGLLLDSETIYTISFSDILINKFSRKEGLTWDVKVQLQGLPGVKACQVVIDSYGLNTETTAEELYKLTSARQEELWPTVGVLPGVQELIEYLKKKDIPICVCTSSYKNKYDLKTERHDALFKLFDGNIITGDNPTIVGKGKPLPFIWWMGLDMINSKNGTEIKPEECLIFEDALPGFISGKRAGGYVIWIPDQRALELISKDEVEKLVGSSNEHGKIYKSMENFRPADYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSS2</accession>
    <name>A0A1E3NSS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70716</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020264.1">
        <property type="nucleotide sequence ID" value="XM_019163719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSS2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSS2"/>
    <dbReference type="GeneID" id="30180406"/>
    <dbReference type="OrthoDB" id="409928at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000006">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17573" checksum="996FC63811DA778E" modified="2017-01-18" version="1">MSLVQLANVCAHLQNVSMAKAPLASIPYTRLHLQIAFGLYKEGFISSLQRGSQNGPDLTPVEVTPDNVSTRRLWLGMKYRQGMPVLSKFQLISKPNRRVYAEAEDLRSFASGNSFRFVKPLMPGEIVFVRPKGAKEVINLHEAVKKQVAGELLCRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSV8</accession>
    <name>A0A1E3NSV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020253.1">
        <property type="nucleotide sequence ID" value="XM_019163716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSV8"/>
    <dbReference type="GeneID" id="30180403"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120019">
        <property type="term" value="F:phosphatidylcholine transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031322">
        <property type="term" value="P:ascospore-type prospore-specific spindle pole body remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120010">
        <property type="term" value="P:intermembrane phospholipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000011">
        <property type="entry name" value="SEC14 cytosolic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="99"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="36424" checksum="51AAAB7F16B67C12" modified="2017-01-18" version="1">MVSEQEVLDTLPKMVAKPPKSQTGFPGYLTDSEKSALEQLRIMLEAEGHTERMDDSTLLRFLRARKFDIMKAKEMYEACEKWRQEFGTNTILTDFHYTEKPTVAKFYPQYYHKTDVDGRPVYIEELGAVNITEMYKITTQERMLKNLVWEYEAFTTYRLPACSRKAGSLIETSCTILDLKGISISAASQVLSYVREASYIGQNYYPERMGKFYLINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYKKELLAQIPAENLPTKFGGSSEVSEAEGGLYLSDVGPWRDPKFIGPEGEAPKPIVQAAAEPQTSRTESLKAPQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSZ9</accession>
    <name>A0A1E3NSZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_19000</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019806.1">
        <property type="nucleotide sequence ID" value="XM_019162100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSZ9"/>
    <dbReference type="GeneID" id="30178787"/>
    <dbReference type="OrthoDB" id="194865at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022950">
        <property type="entry name" value="PPase_methylesterase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" evidence="3">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR022950-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="304" mass="34797" checksum="1E222015F82C9171" modified="2017-01-18" version="1" fragment="single">HGAGSSGLTFANLASCIVQKSDSLHYFTNPGFFTFDMRGHGNTNLINRNNKKLNAMNYDLSIDQLQKDFIFIFNHFVKRYLKSNASILNSGLAKCSLFFLGHSLGGSVLTKVLYKFSKKTEEVSSEFQLDLQYAKFIKGLIMVDIVEETAIRALDFMDGYLNSIPQSFQSLQTAIDWHISHQLIYNRNSCRYSIPPLLTQAKTEQSGYTFVIDLRKTEQYWHEWFLGLSSEFISIPSSVSKLLVLANNDLLDKNLIIGQMQGKYQLIVFRTNDLNLENIIHFIQEDIPNKFAISLLEFVERNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT15</accession>
    <name>A0A1E3NT15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70805</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020362.1">
        <property type="nucleotide sequence ID" value="XM_019163746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT15"/>
    <dbReference type="STRING" id="763406.A0A1E3NT15"/>
    <dbReference type="GeneID" id="30180433"/>
    <dbReference type="OrthoDB" id="5598057at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF1">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="384"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="509" mass="56125" checksum="F858ECD7F96EA7E8" modified="2017-01-18" version="1">MATIEQVLDPNNTTQAQVPGTAPTSGVSQTAAGGSSDLLSYQFPDSTNLVIERYHKYIKLIHSIENFAEGYRQIAASTVKQYESLNKSVTTGMPNFEVSGDEEGATKVSGSAKLGNSAQPEGVDGASITSADAGSPADLNTFLAVMRKKLGDGYTRSMDFQTKVQTQILPDLKHLEAEIDKKQKEYASFSHAEEKDLSQLRSGSAKLTQTLDSAVQEFERGVHAKNRSDYKKDPYLVKKNLLRDAALQVKAENNRIEFLASSETALRAAEARILLELRRLFDLLTQFIDYSYGGTVQSFDTLRDVLNRVPEDLEWSNFVSRNRQYLITANAVDDTVSNPMSNLSLSSAPASARSNYAQAAVSDNSFKRNLEDVTFRNNAHPSTRPVLEGVLARKETTLGLAGKYNSYYFVISPAGYFYGFPSRSIDSFQPNLVLYIPDCETKIRNSSSGQFSFSLRGKDLSNVVPMPKKKYVFKASSAEDFDTWWKVISNYSGEVQSPSALSSDGSDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT79</accession>
    <name>A0A1E3NT79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14722</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">COPI-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020367.1">
        <property type="nucleotide sequence ID" value="XM_019160498.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NT79"/>
    <dbReference type="GeneID" id="30177185"/>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140455">
        <property type="term" value="P:cytoplasm protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000002">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="744"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="713"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1618"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="713"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1634"/>
            <end position="1645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1879" mass="212050" checksum="109A6F592BD1058C" modified="2017-01-18" version="1">MVEEDAASGDQLAASNAVQLENGTPNKERVEKPDVVNGEADSAHVGISDENSKPANAKKAATEEVNGGSEKELSTQEEETPSETVAETVETNGTNEIKASAIDVSASGEDVTNLVKEETAEGQPEPEVAVSSEAQNSIRTFDQQSAYSASNMSPVKKTATTSHLNEAASISFLKSSFESLIAIKEIMKKHQPIVQKATTVIGILKTGEMPPEELIFEPIKLACEQSNVEAKILALDCLSKIFTFNLFTKPIYWDYKGKKNLPIQTDVDLDGSLGSPVQEGKILLIEAAINVVTSCFEGEGTDERVELQIIRVLTGAVVNESMPVHGKVLLQAIRQIHNIFLLSLSPINQGIAQATIIQIVDSVYEKVLKLTSKKKYHSSLQPESDLPDQIENNQQMTLQQLQDTSNDNIQLTSDEIIGNDDELYIKDAFLVFRSMSNLASKTIETESLDMRSHKIRVKLLSLHIIHSILRNYMDVFVNKECLIFNKNSDESTFLVDGIRKYLCLAISRNATSQLSPVYEITLEIYWLMLKNLRSEFKYEIPVFLDEIYFPVSEMKTSASYQKRYILEIIRRICCDPKILVEFYLNYDCDTVLPNLCEAIVDYLARYALLRVVATLPQKLNFKNFQKQHSLSKEIDTIPELNSSKLTSTPPNPDANVNFPNDFALKMSSIDCIVSFLQSLNVSSGASLQLQSSQSVIALDDLKSLSGEARMRGSSIASGTHSSISQSELNINSNESEEASFDPAQFDTVKQRKTSLIEGVKLFNFSPKKGIAYLARNGFVKANDPTSIAKFILENDSLDKTQVGEYLGGSKEENIEVMHEFVDNMDFKNKGFLNALRSFLQHFRLPGESQVIDRFMLKFAEKYVNDNPKAFANADTVYVLSYSVIMLNTDQHSPQVKKRMTLDDFVKNNRGIDDGKDLDPKFLEGVFNDIQGNEIILNSEQQAALISNDSSAQQTLFATALFGRDYASREAYTKVSKVLSKKTENTVILLGKTTKKNNRYYTSDSATNPEHVKSMFENLWMSLLAGLTPPFKDYDDEEIANLLLYGIRLSTHLSCIFNIDYARTSFVRALVQFTNINNPEDMKDKNIKAIYTLLDIAIDESSYLKTAWKDIFIVISQVERLRLLSKGVASKSVPDLLNARLAKKSLDTSHRAESTSFFSLGGKRQSISQQALEHHFNQKLPVDMINKINATELDVSIDKVFSKSSEIQGDGIFDFVGGLVEVARDEIASSGDSKEPRIFCLQKMVDVCYYNMSRIRLQWSELWKVMNQEFNEFGCNPNNIVAFFAIDSLRQLSERFFNIEELAHFKFQKEFLKPFDYIIVNNGNLDVREMVLDCVQYLIIKKAKQIKSGWNTILEILTHVAYSEDSEKFVATGLRFAKQITDEHIESLEEQDAFPALVDCLSEYAKNERFQKISLRALSMINQLVEKIGDEVDERSGDDDYLNKRWFPLLFSFNSIVMEGNDLEVRSKALNFMFEALIIHGKNFDEQFWSKICNELLFPIFDILKRHGQINFTQNESLWVWLSSTLIQALRKMILLFTTFFHELNGTMDGYLSLLISCICQDNDAISKIGISCLEDLILQNTDRFGAEHWKKIEITFKELFKLTSATELFELDPLNKKNKKAASGSSLGAGGNEVAEDESEDDETEQSYSEINPDRKFSSQEIVIKCVLHLHMIQILSELFDNNEFYTVIPYSNLIELTKLLHSSYTFAKNFNEDYNLRVRLWNAGTVDKLPNLLKQETSAVGVYISVLFRLYCDEEKTNSEARDRIVGILIPLAVSLMRRSAEFDEREETKNFHSWMPVVVEVLQAVTELYEKDFKKACPETYPYVMQLFGKNMSVELREVMKDFMVRVGNIYVLHPADTAAATPAAATNGGATGPSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTC5</accession>
    <name>A0A1E3NTC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70941</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020508.1">
        <property type="nucleotide sequence ID" value="XM_019163777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTC5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTC5"/>
    <dbReference type="GeneID" id="30180464"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000057">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF46">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="69"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="515" mass="58520" checksum="5467619D1D6E8B0D" modified="2017-01-18" version="1">MSGKPSIDHESTKERIITDSRSEDSEQQKYSSDNDLLLSLKEKNEKVSQLAEEYNINHKKLMWKIDLCVVPPFCLLYFLAFLDRVNISNAKVYGMERSLDLSSNQFSAALTVFFVPYIIFEILSNYVLKIVKPHLWLSCMILLFGVVTICMAYSRNYGGLIVCRLFLGIFEAGSFPSIFYIMANFYSSAESQRRFSFFFSCTCLAGGCAGALAYRIHDLNGKYGLESWQWIYIIEGAFTAGLALVLYFVVPDFPEECRFLKTHEKSFLKKKLEIYSGNSGYEIKQTWKDVGKVFKDPIIYICAVCYFCFIVPAYSYAFFAPTVIGELGYTAMEAQRHSIYPWLASMGFSIIIAFVSDYFYSRLPFAILATLISITGFGMLIGATDQPLVKYGGCFMLAAGLYTAMPILICWVSLNFSGHTRKSVGTAFIIGFGNIGGIVSSFLFPNHEAPHYTKGLSIGLAFTVFALIMLLVYCSFLAYRNKRKQRQEYKNEWDSLDSREQIMRGDLNPDIKYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTK6</accession>
    <name>A0A1E3NTK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_9925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020574.1">
        <property type="nucleotide sequence ID" value="XM_019165146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTK6"/>
    <dbReference type="GeneID" id="30181833"/>
    <dbReference type="OrthoDB" id="3991453at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="2">
        <location>
            <begin position="450"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="755" mass="87681" checksum="A53F800BD66BEECF" modified="2017-01-18" version="1">MEFIANIRKLPTYTEAESERHFKKFKSSPGLENLEHTKTPALFDDGKANVNSILANDQKHITSSLDDIDNDLLRVHKAARRIYKWSSSQSVYDHGNEILENTKRYIPSLLEKSYRDFNYDGKELLTIFPICKFYQEVWNHFKFAESALYPICHGLNRSVHNRVKVSKPNNIIALNSISYPVTEIQILVCQIFIDKFVHANGDKLYGMQEFDGALDASVSRLCKSLKALLLKELSTKDALKESNKVVQFYLFLNMLERNNNNQEVLLPIFQKSYFHTLKTIKRLIYTDVYYDTIFEKLEYIKNFEHSFTTTLSLALNDPLLTVFNGDEFKILFAQDREFLSSTISTLFLKFFNNLDNEKVRNQLGAVKKFYEAHGFDETDWTIGALKFVSGLYQSLEIVNEDKLKEMVKLFTSLTEIASRIDMFSRISSFAYVKDLFQEELKTYWSKFKRDFAGHYAFCLDYKLKLVSKSKDRNKLENELEKMQVGLKTILGLMNTNSKVKFKRMYVTQFVQRMLGSLLNADFTLYTNISDCELEMCTVLDDVCKGYHYGEPMLEAYQLVQESYKRYKEFGKYLSENDLNHSGIDEIDMVGLPEVLNLSTPFTEIQFNNMVLPLEMGQMMKEFKRFYKNELRRSRRYSEGKLIIQPNYHYSRMTIDYTPPNGIVCHINCNMYQGMILTLFDDDGSENGLVDTDIATKLNIPIDIVLRCLNALGSTKFPILVKSGDHNWKINIDLEIPQKVINSGNTVTITEKKKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTQ5</accession>
    <name>A0A1E3NTQ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020542.1">
        <property type="nucleotide sequence ID" value="XM_019160582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTQ5"/>
    <dbReference type="GeneID" id="30177269"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="57" mass="7296" checksum="B8D026B192642D03" modified="2017-01-18" version="1">MLERQQLWKLHHWVFFDHFIDHPTYSIDFSIHHSVWFIYYYPIDRSIDDSIWIIIHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTQ8</accession>
    <name>A0A1E3NTQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S acidic ribosomal protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_28911</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020533.1">
        <property type="nucleotide sequence ID" value="XM_019162564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTQ8"/>
    <dbReference type="GeneID" id="30179251"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001859">
        <property type="entry name" value="Ribosomal_P1/P2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00456">
        <property type="entry name" value="RIBOSOMALP2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="10548" checksum="6BD291267C31784E" modified="2017-01-18" version="1">MSDALVSYAALILADAEKEISSENLQSVLTAAGASVDSIWTSVFAKALEGKDLKDILFSMAAAAPAAGAAGAASGAAAGASEEAAAAAEEEEKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTR3</accession>
    <name>A0A1E3NTR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020543.1">
        <property type="nucleotide sequence ID" value="XM_019163787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTR3"/>
    <dbReference type="GeneID" id="30180474"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="121" mass="12372" checksum="79B372544ACA3472" modified="2017-01-18" version="1" fragment="single">MNQDGTVDDSVVDEPDGTVDDSVVDEPDGVVDGVVDDEPDGTVDDSVVDEPDGVVDAVVEDEPDLVVEVDPDGLIVGGAEEEPDGVVDGTVDDEPDGAVEDLVVDDPDGVVDDLVVDDPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVF0</accession>
    <name>A0A1E3NVF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">orotate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_30262</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020636.1">
        <property type="nucleotide sequence ID" value="XM_019162633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NVF0"/>
    <dbReference type="GeneID" id="30179320"/>
    <dbReference type="OrthoDB" id="5553476at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046132">
        <property type="term" value="P:pyrimidine ribonucleoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000008">
        <property type="entry name" value="Orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683:SF1">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="50"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="218" mass="23841" checksum="37838B183A6438A5" modified="2017-01-18" version="1">MSYKQNFLQAALDAEALKFGTFTLKSGRISPYFFNMGLFSTAKTLNVLAESYAHAIIDSGIEFDVLFGPAYKGIPLAAITVTKLYEIGGDKYASIGYAFNRKEKKDHGEGGSIVGSVLKGKKVLIIDDVMTAGTAINEAFGIIKEEQGNTVACIIAVDRMETTKDSNECATKIVSKRYGVPVISIVSFNDIVKVLQSKFTTEQMASIDEYRKKYIPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND98</accession>
    <name>A0A1E3ND98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015214.1">
        <property type="nucleotide sequence ID" value="XM_019164923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND98"/>
    <dbReference type="GeneID" id="30181610"/>
    <dbReference type="OrthoDB" id="10273865at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="152" mass="16598" checksum="D6166B0C1DD37E99" modified="2017-01-18" version="1">MTIGQVAAWQILPDDGSTKVAGYNTALCILAGGFDNSTIIAQGDNGGMVIVNPQGVSNQTIANRVIMLCRERFGNNITLSSYRYSNYEDYYTPIANIVEALDNIDLLPEFSRDTYDPAQYNYLATGNNYQRTQANGFGRAGPWNGLAACHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDA3</accession>
    <name>A0A1E3NDA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_37730</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the interconversion between the alpha and beta anomers from at least three hexose 6-phosphate sugars (Glc6P, Gal6P, and Man6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015215.1">
        <property type="nucleotide sequence ID" value="XM_019163102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDA3"/>
    <dbReference type="STRING" id="763406.A0A1E3NDA3"/>
    <dbReference type="GeneID" id="30179789"/>
    <dbReference type="OrthoDB" id="1659429at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="298" mass="33552" checksum="17B524B69F5E2DD8" modified="2017-01-18" version="1">MAVEDLGDTVELTFPSSPKTRVTILKYGATVISWKVADVEQLWLSEGAKLDGSKPVRGGIPLVFPVFGKSAEEGFQALPQHGFARNSTWEFLGQTTENPPTVQFALSPAEANPEVYSKWGKGDNDFTLFLTITLNDDNLTTKIEVSNSDSKPWTFNWLFHTYLKVNEIEDTLVNNLPGEECYDQLIAETYEEKSPAIDFTQELDRIYRNVSTDKVVQVIELGKVVHNVKRVNLPDLVVWNPWVEKSKGMADFEPKSGYHKMVCIEPGHVHDFVELKPGESWTAEQIIYKGDAIKLQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDH1</accession>
    <name>A0A1E3NDH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyrroline-5-carboxylate reductase catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74779</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015283.1">
        <property type="nucleotide sequence ID" value="XM_019164867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDH1"/>
    <dbReference type="GeneID" id="30181554"/>
    <dbReference type="OrthoDB" id="550646at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133313" description="Pyrroline-5-carboxylate reductase catalytic N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="267" mass="28660" checksum="23C49052E0FAC7E5" modified="2017-01-18" version="1">MIQTIGFIGVGLIGSSLARLAVDAGYKVVLSNSREPASLERMVELLGGENVASADTPQNICSNDNIKLVVLTVPLNVIPSLIPALGGLKDKIVVDTNNYYPHRDGHVEALDSRKLNTAEYVAQYLDKSAKQIKVFNNIWAIHIQPAASRDSTNQTYLPVAGDDVEAKGLVREFVKSIGFGTVDAGSLRDSWKIEPNTPVYCKPYAPVLPEGLTEQEAVRYFGSHAAAPLTEGELVLLVSAATGNEPVGGVFDPNSISSRAMINNYAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDM3</accession>
    <name>A0A1E3NDM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015342.1">
        <property type="nucleotide sequence ID" value="XM_019164888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDM3"/>
    <dbReference type="STRING" id="763406.A0A1E3NDM3"/>
    <dbReference type="GeneID" id="30181575"/>
    <dbReference type="OrthoDB" id="1929311at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030:SF0">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="320" mass="36800" checksum="FEE2B21B5840BB02" modified="2017-01-18" version="1">MSITEKKEKSERAVDVEGLDDGLAVDYDLSENEEGTEVKGEAAEDVAGEQEGEPKEEETTVKEEKILSKKRKVTEAQKSKKKQKMEFEKESKKALSSEQTDIIVEKLSSKIREIFPQLSALELTDYYLSKSNVVDTNDFTPERGLAFFRQFIEMYMEDLVPSIKEYKKLRNKIKKYETKKRFNKNFEKTVTIPKRRFILILSISAIRACDVHRATRDIEGGSIKLIQKNPIGQDLKMLRTTWSRVLNATPSRVDKILEISKMDHEKNPDAGFSLKEDEIDAVVLDNYVDPKLRSVLECGETFELLKKLKTANPALKVYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDM4</accession>
    <name>A0A1E3NDM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein STU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_13773</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015344.1">
        <property type="nucleotide sequence ID" value="XM_019160111.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NDM4"/>
    <dbReference type="GeneID" id="30176798"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060172">
        <property type="term" value="P:astral microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CLASP N-terminal" evidence="7">
        <location>
            <begin position="26"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="CLASP N-terminal" evidence="7">
        <location>
            <begin position="299"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1295" mass="148433" checksum="DA2D21B9A7A1D3AA" modified="2017-01-18" version="1">MQNYHDYDAGAVMLDAVHADDLSTVETAITNFKQYIKRNLVDLDYALPYVTFLISMYETKDLAVLSFSTLCHLIKRISIQDPSVLQHVYDPVVPFLLHRLADHKDSIKMTALKSLKTCIDSCVSRNIDSLIRFLIQDGLMIDDAQIQITVIDILDQIIDPNSNFNFSFKMVLSASVKLLKSRNFTVSSKSADLIKVYFTLINPNNNTAKSDLLNDLISNDVPERILTNLLKSIDGSLYKKYISLTNPDQSSQVSDLASNVDKLNYMLDQTPNWNIDDSSLSVVGENDDVNYEALLFESEQLFEGKETEKNWKSRQQFIIKVRQILRGKSFLLNIDSSLAFVKEIRDRVTKGMLSLRTTLSNNSCQLCKELVIHMGSFLDFTFIEPLLMSLLRLTSARKIMQHQNANVAIIAILLYINLNPKVFSILNATIQEKNIQPRVYTGNWIQLILLKYYSPQNLENFYFLVESIEGIMIKGISDPIPQVKDAMRNAFWTLCEFDSSYETKITKKLDFAIVRALERSKNVYLNSNNIKSKQMQKREPIKDQISDFEKEEHLPPQDSYPISRKEQNKVMDKENIQLQYEPIRKSIRSESLDEARMTQKKKQHVLRNHTVDVNPSATILETNYAASTNNDDLVDEFTGRIKRENVIYEEITSDSKALQAEGFSKLLKENDSSLTIKFHGALNNLTILNADLFNLIFMEGNEKFFRKISSYLSTENTLRLFCLYLIKSGDYNRIDFVINELSLEDTCLSIINILNLSIDASKIDNINLSIQYIKNRFKIIHSILKIFNALIALKRNIIKSYLLSSIFECLFLSFGIVDDDLIKKEYVSTFNICLQEYDDLFKRSLKEVKDPALKKEIHISLSLPVDDEDADQGDVEMFKEVDGEKEKEGENTKFLSPFKIDAEEFDDNIDGMTKVIPKLKSFEPGNENKKQPLKNTVVSDFTMIFPKHAGSKLFDFDKIEIKHEETDRDMSVGVDEVISQIEKDDERSEMEDVEEIGSEVGSEHIGDAEDRAEVAPKTMTEGSRIESDEPFEGKEFAAEETHDDIMPEKEKSILSDSTPDMNQLTIDENKEMGGYLGLVVKEILQYEPAEDFKAASVLNSLNDIETLSWVLSNADSVDRSELYAKLAEQLRGEHQIECLAVLKYFYDMWPEEIDASVIRVFNEIISNTTTATELFLGVLEIVELVDVKVLVSVGKIHSLHPRLQEIVLRCILQRMQSNRVDCEDIFMIERIINRGCEHDDSFIRMNSYLIYKEMYRMFIDKTCDSQGIELVDYVTVDGMKDSIKLFCGVPVSLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDT8</accession>
    <name>A0A1E3NDT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Membrane anchor Opy2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_13496</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015402.1">
        <property type="nucleotide sequence ID" value="XM_019160067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDT8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDT8"/>
    <dbReference type="GeneID" id="30176754"/>
    <dbReference type="OrthoDB" id="2402916at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018571">
        <property type="entry name" value="Membrane_anchor_Opy2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09463">
        <property type="entry name" value="Opy2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane anchor Opy2 N-terminal" evidence="3">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="510" mass="56062" checksum="361A40BF268084F3" modified="2017-01-18" version="1">MVVVIEENYIWDPVFISKREDHLTASSSQNNAVSMSSSSSSSQTSGVDALLDSHGCVICNSTPSCPQCKSYQQCAMTTQTCAQCPTTYCLDNSDGTNSTGVNSLNSAEIGGIAGGLSGFVFILLLVGIYFLYKKYKNHLLVDYELGIGEEMKELTGFNDYDDGFDDGAGGGYGDGVRNSVRNARSSQFLHPGNAEKRMSQTSLSTVTNSVLTKASNVLNIVYIPGVTNSRPARPIVGGSRRNTRRAVNSTYSRGMSVYSKETYFSDLENASFHGGNVATRGGNPTLVEINQDDYNYDDEDKGLRDEEEEVNYPININLQLPPLPERRQMDDDIVEEEDEAEDEMERRERSNSFIDGYESDIDDTHQRRKEGGEGERGRERTFIGEEEGESGEDAEDAIHLDIGLSDSNEHIANSNNHSNGNYSRTVPNRYAALVEKKDQLNYAHLGPFASSKDAEEGTDLHNESYSDSSSDSDEENIEMLLHQSGQSSNSNTNSNLFNSNTSNPFLSPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDY6</accession>
    <name>A0A1E3NDY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18258</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015453.1">
        <property type="nucleotide sequence ID" value="XM_019161926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDY6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDY6"/>
    <dbReference type="GeneID" id="30178613"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF37">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="371"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1021"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1021"/>
            <end position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1156" mass="128035" checksum="B22DBF1D74BB8983" modified="2017-01-18" version="1">MALASGEEWSQRDGVLVGAGVSLGSCFSPSEGELALRRWSRTDRLYQRERDRSAQEGAVGANRLDEWGSDVMWNHLCRDPCAPLPLPMMTDAEQMDESKKTQKACDECRSRKVKCDGLPTCTRCVNKGFPCTYNYVFKKRLRKKMQEMVNQPKRRRGRPRKSSIVNSPLVSDRDPAAEAAGPASSNNTSTSNLGSTSGRYAGSLGASTPASTVGAGAAAAVSAQQYLPQPLPLPIPMSTSVSAPLGAAEATFPYPVANNTSFAGAIPSYDAGFYRNTVPSNPLALSQLSGSPAPAPAPAHATEQDSSVEQRLQKMESMISLLLDKVSSKSPRPSALSAANFGPSQAGTGGCAGALSLTGVNLDASSARATSDIASNSNSNTHASFTMNHNTFSGSERSKANDSPQLFESGCSSLTNPESLQKWVGLLMHTSIFFLSPIGMKTLENKMENPEALAPLKQIVQVSTPCERKIISIWTNPIYESQLTPLPSRANIECLLQALSAPFFLLKVLDVGYLKHLLRLYCDFRDGLIPEPYFSYSDYLLMNSSLLVACLVIEEFSEKALNGGGPMPDFKSLREVSDNLLDNALFYYSRLSVISGGVSSIVGSLFLAFYADSISLSRAAYLISSSAIRQAQELGLHIEETYRTLPQHERALRLNIWWACYVFDRELCIRWGQTPVINDNDISAPPLPGFEAYWSPNQTAEQGPSKRSIYHFEIQTLLEPLLENPSSSIDLEQYISTDYAFITAKVYDNFLRAGALKNVSKKEAKERLIHTIAELDYWKESIPKPIRPRTKEDDDKSFYEFIEKLTLTNTMYSLHLMIMATSLHVRYHHVKLMVFRAYTKHCFVEYGDRIDDELLSEPTISARNILHMSCVVDSRFGNYVNYFIFYPFNAFLSICGLYILLEKEPPDLRSDLQLLIDSIKLHFTPFVSNKKHHEKGGMVELVLKGMLYATYVTCKSRFGDIPLEGLEVLDDARAIAEGNKTAAEIVCPVLNMIETRVSPERPLCKLPYFYNQNPSLFITKDDNSHSRESPLHSASHSPIDRPSQLSDIPILVGNDTDRTPNVSFLLSPSHQPTEGNIRLNPANATTVGFKAEGSEDKNPFLSNGAVKLDKDPEPDYFNMSDVNSKDTLFHNMLNIPNYFFDYLYEDTNNTALTGFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE28</accession>
    <name>A0A1E3NE28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PICMEDRAFT_18305</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms DNA polymerase zeta with REV7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015503.1">
        <property type="nucleotide sequence ID" value="XM_019161942.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NE28"/>
    <dbReference type="GeneID" id="30178629"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000007">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="25">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="24">
        <location>
            <begin position="181"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="785"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="1019"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="108"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="ODQ44390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1602" mass="184817" checksum="5CB12942D8686960" modified="2017-01-18" version="1">MDVRIITYDSYQSAPTPLDKSVVTINGKTEPSITKFYINRVPIIRIFGKLPTNHTCLLHVHDVFPYLYINFPKANGWDFLALSPENIAELDRWLVEINKALSESFKKKHKKPRTYRKKAKRKYTKERDPADQVLGGQDHTKVENDYQYGNGEVDVDVDDLDDDDTDDDDDCSSDESCNDFFHSYVSDLSIVTGMPFYGYHLQNMTFLKISLTSPKYVTRLARLLGESKVFNKFIQPYESHVPYNLQFLIDYNCYTLKEINITDYIWRTPLVIVNDSYNVAAYQTFFETYFTRFNSQKIDSSLKKFLNEHLYPLNGRINVLDPESLPRIGRTMVELDIVAGWIINRSSLHERKIPGLCDNVLLDDKVQYISSTKSLLDDVDNVRKGRGLQIGGSQLGLFDKTPRSVSSSVRWNDQEEIDKLFSNCVKLSEKSYYEKFNDSEFTRLKSPKPEASFPSSFQSIGILHHFPDPNPKYLLNTDMIDTKSDDIFDQILICSKSFTSNSSSIIENQNIFDLTGHNNVGDPFSYPKDEVIESSTENEEVEIIDESNEISTNPDHSVSNLTEKLQGASTLEESVVFDNSDLRFLESTQSDNIKKKLREPSIFTQREKLSAHEIFTNYFEDSDHGEKIYSFNLQQPKVHSKDDFLNSFQTNYHMLKIEYPDPFYSKLSNFDSKPFIFAGEKFQLNCTEVDTNLYPSGTQLNTFTKGTDDLFIWNYTPKMPSPADVKSWLDTEKVLDPPSFVFHSQIRGPTQKFKGFRYASLKTPPERMANSSLTMDQLLLIVEIHINTSGNKMPDPKVDAICAVFWKTNIENFTFDQDFPASGSLVNTDGNIAEELSKISNQTELSITRYDSEYEMLIGLVSLVEYLDPDILVGFELHSLSWGFILERAKYKFKIDLCQRLSRVVFKHNNKIGDRWGYTHASGIRITGRQLFNLWRRLRSELNLNHYSLENIIFHIFHVRIPTFSWKQLTNWWVSGQGKYLSYVINYHNSRIEYEMELITKLEIVEKINEQSKLLGIDFYSMIYRGSQFKVECLLVRLAKAENFMLISPSKKQVFKQDSLECIPLVMEPNSAMFKSPLVVLDFQSLYPSLIIAYNICYSTMLGKLKNYDPYKYTKMGVTNYKTPEGILKLLESDINISPNGVMFAKQNIRKSVLAKMLTEILNARIYIKSTMNEFKDDDVLRKLYNNRQLALKLIANVTYGYTSASYSGRMPNSDVADAIVSCGRETLLKAVKEIEKTSKWGAKVVYGDTDSLFVYLPGKTKADAFRIGKEMANHVTSLNPEPIKLKFEKVYLPSILVSKKRYIGWSFEHESQVNPKFDAKGIETVRRDGIPAQQKILEKAILILFETKDISQIKDYILDEFLKIVKNKINLQDFLFAKEVRLGTYKNEKYIPPGARLSMKRVQKDHRSEPQYKERVFYLVRKGHNKEILRDRCVSPEDFLNNAALELDSDYYINKVLIPPLERIFNLFGVDVRAWVKEIPKMLSYTGVGVTLLNVKVVSCVCCHKSENLKTSDDSSLCENCKKEELRTALTLKNRLKVKETNCMNYINFCRSCSAYAVGEAVNNKVAKSCCNVDCSVYYTRMKVTRTTDVMIRKYQKIPDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE78</accession>
    <name>A0A1E3NE78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CIA1</name>
        <name evidence="6" type="ORF">PICMEDRAFT_37084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015532.1">
        <property type="nucleotide sequence ID" value="XM_019163056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE78"/>
    <dbReference type="STRING" id="763406.A0A1E3NE78"/>
    <dbReference type="GeneID" id="30179743"/>
    <dbReference type="OrthoDB" id="284782at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="358" mass="39983" checksum="65464ECFD5AA81BD" modified="2017-01-18" version="1">MGNAVKQAEELRLLAKIQAHDESVWNMSVHSKLPILATCSSDRTSKIYDISVLENVKLTTVLDEQTHTKTIRCVSFKPSSEEAYPTLALGSFDSTCSIWGADSFKSEWELLAVIEGHENEVKSIDWSLDGRYLATCARDKTIWIWETDSMNEEFECIAVLSEHEGDVKFVKWNDKGEDTHVFVSCSYDDTLRIWRQDEYDEDEWNCVAMINLENTVWGAAWVDNDTIRVLTEADKAEAEQGQDKDDQETTDSSKKLPSTIKKIETWSASSAFGKVSRMHEGTVYSVDSRGGRIVSGGSDGCVYVYEREAAGPESGEWRVCASHSLCHGVREVNSVRFVRDNTVASGGDDGSVAVWGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEF7</accession>
    <name>A0A1E3NEF7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015589.1">
        <property type="nucleotide sequence ID" value="XM_019161975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NEF7"/>
    <dbReference type="GeneID" id="30178662"/>
    <dbReference type="OrthoDB" id="10263222at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090730">
        <property type="term" value="C:Las1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="473" mass="55727" checksum="EEB0337675FE4B48" modified="2017-01-18" version="1">MNNPQLTPYKFPQEAEKLYKDFFHSRYPDQKTQAISKVRSYLTKGPIPHSIEITALLTEAILEDEKKTGANLSTYEKSYPRVTSTSSMSDLSIRLQYSMCIIKFVNGLLDPFQQSIYNISLHKLAIELNLPNYFVETRHVSTHERLPSLEMMRLVALRALNWIKSEYWEKAIGQYKEQGALEVNLDQWIECIKNERRIRIKRENDARRKENLLFISEADIEELESVLKNIKKIRKEELQKNRKVKELVTEIGKLNKLIASKPNEFSIQLLIFKNYLILHGEKNENLNEKKMLGLRKMWGEVVEKLEPDFCFKLWTFMFKLATMKTFVKYDQGYVESKLLANQTIQYLQDDREYTQTVGWLIDMLTNVGFVNKTNIESLLNLLMISSDISKKCLPILKQKYYNFLTECKLFEKAEKLESIMTRFWVIDNNFAEKKIQDIDHDAGNYVQNKRVKPSLYLFETFPSWRPVPFGCPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEH1</accession>
    <name>A0A1E3NEH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase M16 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_74754</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015637.1">
        <property type="nucleotide sequence ID" value="XM_019164861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NEH1"/>
    <dbReference type="GeneID" id="30181548"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000004">
        <property type="entry name" value="Putative insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="8">
        <location>
            <begin position="37"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="9">
        <location>
            <begin position="199"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="10">
        <location>
            <begin position="429"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="11">
        <location>
            <begin position="809"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1089" mass="125505" checksum="53C8E5F3B9D291FE" modified="2017-01-18" version="1">MSSQKHTIVADDGAIAKPDIDDRKYRIVKLENQLTALLIHDPATDKSAAALDVNVGSYNDPKSLPGLAHFCEHLLFLGTKKYPQENDYNSYLSKNSGYSNAFTSSLHTNYYFEVKNDALEGALDRFSQFFIEPLFSPSGKDREVNAVDSENKKNLQSDIWRLYQLSKSTTSSLHPHNGFSTGNKVTLGDIPAENGINVRDELLKFYENNYSSNIMCVSILSNESLDTLTDWTVNMFSNVPNKSLPKPFYEHSPFNSENYSNKLYKIKPITEMRNLQLTFPIPATTQYWEYLPNNYLSHLIGHESKGSLLFNFKKKGWANGLTSGASQISPGFSEFNINIELTTKGLEKYDLMIEDVFKYLKMLKTEKPQKWIFDELRTESLNSFKFRQKGAVAATVSRYAGQLQDLHYYSVPMKNPLLDLDATTVSSSNIPPEDLLSLSVTRKYDPALIDEILSYMNPTNFRVFLISKDILSDVEESKIEQEKWYKTEYSVETYNKIKDLDALPLDPHLTLPEKNEFMPTKFELVECDSTKHPKLVELDQFSKIWFKSNSLLSGPRSAITIKFNLPGSTSTPLNSLYLSLFVELLDDELNSLSYYASLAGLHYVFNLAREGISLEIIGYSHKEEILLTKLIQTLNTFTSSDQEASVWSESRNKRFELLREKLFRSLKNFGYATPYQQVGPIISSLVNENSWLIDDEISCFNAVDFNSLKHYSMNLFKICFAEVLVIGNYEKSDTAKIHNIIKENLPTLNSSITLTQSQFTRGRSLYLEDNSLSHYLKPNDDPDNINSCIELYIQIGLIPNHRDRILNELVSQIVHEPCFNRLRTIEQLGYVVFSGTRETRTTFGLRFLIQSEYPTFYLLMRINKFIKKMGIYIEQKMTSDDFAKHVEALVNKKEQKMKNLREERNYHWNRIASGYYDFDRYEKDVEFLKSFKKEEVIDYYIHHILDSANNGKLAVHLQSQKLPALDPLKMVKNSFSNFVYDKVEFDNVNYESDKVNEQIDEFFGKKAVTRESLDEFLTDKLFDEFPFKKELADEILFNLTKDWNSYDKKDGSIVDNVGEWKCSVPLTVAPTAKIENKHCDKEFAFKGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NET8</accession>
    <name>A0A1E3NET8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_18063</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PGA52 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015770.1">
        <property type="nucleotide sequence ID" value="XM_019161864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NET8"/>
    <dbReference type="STRING" id="763406.A0A1E3NET8"/>
    <dbReference type="GeneID" id="30178551"/>
    <dbReference type="OrthoDB" id="118256at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018805">
        <property type="entry name" value="YJL171C/Tos1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018807">
        <property type="entry name" value="YJL171C/Tos1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737:SF2">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10287">
        <property type="entry name" value="YJL171C_Tos1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10290">
        <property type="entry name" value="YJL171C_Tos1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133334" description="glucan endo-1,3-beta-D-glucosidase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 N-terminal" evidence="11">
        <location>
            <begin position="39"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 C-terminal" evidence="10">
        <location>
            <begin position="241"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="481" mass="49531" checksum="530BEA6AE7DCFB5F" modified="2017-01-18" version="1">MRFSTSLLYSIATALVSTSTVDAGCQYIGGNYYCNSVSAVQYQNVGFSGSYNDVTNMDETTCQCSSSPKSFSGTLSPLDEELSIHFRGPIKLAQFGVYYPASSKMAKRDFSDADAAAAAADAPVADASSDAAPCVTTKHIHHRHKRAPVIEYEYVTETVPAGQNINPAGPSSSTAPTGQPAPLYGPASSSGTTLLTSTLSQSYAPGPNTATFVSSGSTTGASPSASSSSSSSSSPSSGSGGDWVRSSYFTPGSADGLVFLNSKGGSAGSGTWSSCFGNSLSYCASDGVNGAASAQALGDVTIDSDVEFMIFHSSLCSDSSISDCGYYRSDIPAHHGFAGAEKVFVFEFQMPSNSGSSFNGDMPAIWMLNAKIPRTLQYGNSACSCWSTGCGEFDIFEILSAGSDKLISHLHDGQGSTGSYGGGGTSDYFSRPYGSTMKAAVVFQDGNISIVVLDDDTTFGSSLSASTVNGWASTSGTQVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEW2</accession>
    <name>A0A1E3NEW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18068</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR48 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015775.1">
        <property type="nucleotide sequence ID" value="XM_019161865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEW2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEW2"/>
    <dbReference type="GeneID" id="30178552"/>
    <dbReference type="OrthoDB" id="2421129at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051246">
        <property type="entry name" value="WDR48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021772">
        <property type="entry name" value="WDR48/Bun107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862:SF14">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11816">
        <property type="entry name" value="DUF3337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="170"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="211"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="297"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="885"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="885"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="897"/>
            <end position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1193" mass="132746" checksum="362F8A4597F8B1FD" modified="2017-01-18" version="1">MSFFKSTFSSNPTAGSGANGGSSNHSSHTTATAKLPSNASPRQPEPAKKKSLTYILNSPNERQMKGHHSIGVNSLLYNKTDKCLISGGRDGQISIWKFDTDADPSKYDPIKDNDDVYDNFRTNADIRNHIFQNLDNDLELENLETSIKQGVSSVSIPTSTSAPHYYRNSFLHHFGWINDMALLDDGHTIASCSNDLSIKCWNYSTGTKSTLGYHDDYIKKIGYTKSRKNELVSAGLDKVVKVWDTTKGQPISSYKFKDFNNSIYSLDVNNDMIICSGPSNIINLFDRRGMAKPVKTFLGHTDNVRALLLKDNSFLSGSSDSTLKLWDLRSTRILRNFEMHNSPIWSIYTPDYSDDFSIFYSADKSGLLLKTDLRAADLNSQNLSGYFNYKVNESLGISTVIANVNYSNTSPDNSSVADMESSVSGINDIVETDDLGTLWTATASTIHNANTNYISSWSIPQTGKLVIHQGLVLNRRLASLYNNNNNTSGNGEALTPTSNIGQLSGFHFKRPNSVQNDLQIDTVKSNDSIHDTEDLVSQLSGDDLDQIDNALFSNTNGLDKLLVENSPQLDGKTDQSVTSYDIGGEYDPVGTHTDTGDVYGERVEDDEMDEDGYEYATCFVELWGNLNTHFLFADDYINTLEQEIESLAPSQDTTGGYDPFSGPPKVIENNLQVTKKMVINCGNVNEDEILLLPFNIKPISKISGTSGLIRSKILNNRRHVAAMDQTGCVYIFDILQCKLIHRVDNHLSVNSIDTIENIKLKSDATREETFTATNEDELNLSDRFEAVCEKFQTQETLPTWCSVQVKAGQLFITLKENNFIACEIYTDDFFEYYRELMGQAQQISNLRVDLGKIVVKTFFGNVLDSYLLKIIPNFNATSTEKLSLSDSTLDSGATDKSSFSNDVSKSSSQSRPQSSSSTTSVSAEKTSGSKRGLFGRLKQKDLRKDISSSPSSPSTLGAKAGPAHSKSKADLMVERINHIDDTSKLLKFLELNPEAIPMVKAYQEGMDDRRNEEIPVFNYVPENEGEEKMEAEGEEKRGVTETSTLVVINEETQLETRPAYTVYLNELVSRNIDEEEVVKNIPIWIAKGLLLDLYPIPSSMMMKIGFSLVPMEGSNLSRLNDEKSMRLNAVGTLRVARILDFVRSKLPAEEARAPLELVCKDAVLGEKYTLGTIKARVWRQGGDIEIFFRKRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFG4</accession>
    <name>A0A1E3NFG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Urea active transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_17396</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015977.1">
        <property type="nucleotide sequence ID" value="XM_019161584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NFG4"/>
    <dbReference type="GeneID" id="30178271"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000006">
        <property type="entry name" value="Urea active transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="636"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="721" mass="77796" checksum="6FB17F6F5A579744" modified="2017-01-18" version="1">MSDIEVPLPQGAGYAIIVGLGMVFSVGMILTTFVLRRYQKEVITSEEFSTAGRSVKTGLIAAAVVSSWTWAATLLQSSTQAYKNGISGPYFYAAGACCQIILFAFIAIKAKQKAPGAHTYLEIIKARYGPIAHAVFIFFAFATNVLVTAMLLTGGSAAVTALTGMNTVAAIFLFPVGVMIYTLFGGIKATFLTDYIHTIAIIVIILTFAFTTYATSPLLGSPAKVWELITQAGIDHPVSGNAEGSYLTMHSRSGGIFFVINIVGNFGTVFLDNGYWNKAIASSPAAALPGYIAGGIAWFAIPWLTATTMGLACLALENSPSFPTYPHRLSDEQVSAGLVLPSAAVALMGKGGAVATLFMVFMAVTSAMSAELIAISSIFTYDIYKGYVDPNASGKRLIFTSHCTVVAFSLAMSGFATGLYYAGISMGYLYEIMGIIISAAVIPATMTLLSKHQNTISVIVSPVLGTAVAIMSWLVSTHTLYGAITVDNTFMDNPMLIGNVVALLSPAVFIPILTLTFGSQQFDFEILKSIKRVDDSAEVEEDLKDIIPEGVNAEEESENSMTKDVEKINPVKSNVTEIVDHLAHDSKVLSEARAEEQHLKRASKIAGWLCLAMSFSLLVIWPMPMYGRSYIFSKHFFTGWVVVGIIWIFVTGFIVIIYPLWEGRVGIFTSFRGIYWDMTGQGHKLREWQTAHPEQLHAVRSQISAEISRNGGAEDIDEILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFK7</accession>
    <name>A0A1E3NFK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_12670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016027.1">
        <property type="nucleotide sequence ID" value="XM_019159955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFK7"/>
    <dbReference type="STRING" id="763406.A0A1E3NFK7"/>
    <dbReference type="GeneID" id="30176642"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000181">
        <property type="entry name" value="CALML5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000251">
        <property type="entry name" value="Calmodulin-related protein 97A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="149" mass="16571" checksum="7E9D484FB461ED4B" modified="2017-01-18" version="1">MSDKLSPEQIEEYKEAFSIFDKNGDGKISPSELGTVMRALGQNPTQQELNDLVNEIDSNGNSMIEFSEFLTMMARQIKEQDVEAEILEAFKVFDSDGDGKISQTELVRVLTTIGEKLTDAEAKQMLEAADTDSDGQIDIEEFARVLKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG15</accession>
    <name>A0A1E3NG15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytosolic Fe-S cluster assembly factor NAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cytosolic Fe-S cluster assembly factor nar1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nuclear architecture-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_12813</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016187.1">
        <property type="nucleotide sequence ID" value="XM_019159970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG15"/>
    <dbReference type="STRING" id="763406.A0A1E3NG15"/>
    <dbReference type="GeneID" id="30176657"/>
    <dbReference type="OrthoDB" id="10253113at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase large subunit C-terminal" evidence="7">
        <location>
            <begin position="128"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="507" mass="54472" checksum="C8DE55657B238F63" modified="2017-01-18" version="1">MSSLLSADDLNDFIRPGVACIKPVEDLRKVPEGGPRHVELEFESGDGGGPAEVEVEVGADGEMVEVEKGGSGSRRRLEKAQISLADCLACSGCITSSEEVLISQHSFQEFVSFWTEHAAAQAAGELHYVLSLSQQARASFANAFDVSVEVADRVLARVFVEHYGFEYVVSVGVGRRLAYAAQYDEVEARTKSEPEPAAGAAAGANVLASICPGWVLYVEKTHPELAGRLSRVRSPQYITCRLVKDVLRRERGYERTQVYNLSVMPCFDKKLEASRDDDVVECVITPREVVSMLLEDDRVDLERLIEDEAAAAAAAGAADYAAFVRRVSPPGWFAGNMYGWGGDAGDESGGYAVAYLEQYRRRHGVTDGWVRTVRGRNEDVYELQLVRGGGAGPEEVVCRSGVINGFKNIQNLVMACPSGCINGGGQLTGSPPVSPAAWLARARARYAEVPAWAADSDDEQRRLQRWLAQYGGGQDDVVLARAPSSAAGATGAKSDAEAAVAVASAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG30</accession>
    <name>A0A1E3NG30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inheritance of peroxisomes protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016218.1">
        <property type="nucleotide sequence ID" value="XM_019161664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG30"/>
    <dbReference type="GeneID" id="30178351"/>
    <dbReference type="OrthoDB" id="3997626at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="728" mass="82450" checksum="3FFFAC9613EE1094" modified="2017-01-18" version="1">MAGSTGEARMPAIPTTSQVLQNTMSMPAPSFSDLVTSSTSAFTSTANSALTSASTTPHLASDGTLHSYKFERSAKCASQSDCEDEGQETDNTVIHYSNEETTSQHTLNDADFSAYAKGYIDSMNFELEQDYDNTTKILDGHTRRNLSIEDITDSYIFILKQSQVDLKNEIQRAFEEFKFDIITDHLSPPNESIYYRTASGPNRTTSEHIGRHVYESLDGGEETVSIRLSDTPYLILLHSIRPQLMSFSILSFLRKKLHLLEKRTILSLLTLAVSIINMANNSNEGLKLYSKMLIYRTQKLTYSLLQLERFLSTSVSAKNQAKRYNLTIGGNNNSSKRAKLTLISSAIHLLISVFVKRIGKMITYISNVGSLWKYIAVYGLDGDFDEIRIVRNIIQNPESCSCTWSNEPERLVRVLQYVRKMSLCIILSLMEVEENEEKDNVKATIFMKNFWSNFGFESVNWNVGSPTLATRLLGMCTCVEEFTTFVDLMKEEIVAEEVQLVDSNEPGELSEEFNENSRSDNIYGLGVLRERNEDERIRELSSMVGKVGYKLDLIELGMDSKEGIFTLKSDIDELVRCYNEVTSGKASRRNENTTTKDKDLKRLRMSEILLDDFDGAAEKNRRNRRSSGIDLKLFSVVKTTDEVKPDRCEIVQADERSSDTEFRKTLERLCLDRGAKAISRTGNKDVGEVEAEADTDLAMTRTGKTHTEEAAFLEFKNELRKRLEEERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG66</accession>
    <name>A0A1E3NG66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_173471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015671.1">
        <property type="nucleotide sequence ID" value="XM_019161555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG66"/>
    <dbReference type="GeneID" id="30178242"/>
    <dbReference type="OrthoDB" id="3995391at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="622" mass="71140" checksum="85C3A4B3CB43CDD0" modified="2017-01-18" version="1">MMQKQKDNELSAMEHVQKLEEFINSTEIENLQSMSQADKLEYILSSKDFMANMAAGLLMQKDTAILGDEDVNFDSDIKGDFSSAKNSISSIADSNMNFLTRFSKEEKLEIQKKLSIYDHTLKATLDAIKSGKVKNEQALPPLFQNSPIDADMNQLLKSVNSSSVKKLKVNNDISVSIETTSDSPSNGQNPNSLQYEAKTKEAADKYTEQMIKIINHHKKFDNFFDGVQQIEDPSDRSKNFENNEKYGSSSQDESCKLTLEYDKNGNLIHTGSEDALKTKINAQIVKALETLKLDYNLEEDIASAIAKSFENNHHLLPKAEESNFASDEELDKKGLSIDTFTSQLEQYKERYMQQLEGLSNSAEFGSYASKANQHSVQNSSEQLQNQQFETQHSRPHNAQHKDEERESTNLKGQVRHAKQSKINRSRRETPKLEYYTDPTPEQKQKLRRECMEKLRIQDLQLNEEVTKKSKKKKNKKKKNSNNSLATNGIGNTYIGNSHSDAWLCELCEYKIVYGEIPVFLTEWLQKKANHHEKMETYQRYLLEQRKERKLQQNRARPDCQNPNHQHEHDGGCSHSHTHNHHLEQHHGNCHPHDEVLSAHESLSFSPPPPPPPPPPPSSSITW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGG0</accession>
    <name>A0A1E3NGG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015771.1">
        <property type="nucleotide sequence ID" value="XM_019160042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGG0"/>
    <dbReference type="GeneID" id="30176729"/>
    <dbReference type="OrthoDB" id="3990850at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="1">
        <location>
            <begin position="83"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="220" mass="26297" checksum="1B56C624ACAC2DC8" modified="2017-01-18" version="1">MDTASNNYVTQPEKFKVAPFPFFLCEYCQKISDTCYFCYNRPCNFIRKLQQFQLYNQPNGFTIDNVVYHCDNDYIYEENVDNKDRIYDPQITRCKIEDEFVNGKKTKKDHPGFCKYCTMYDENWDSNFYERNNSRYRGHMINTHGIHPNGAICKMPDTGYFCYKWVRNHWFETSGFFCPYPNCNAAFAIGEKGHGFHEYLRHWSKVHVKLEPQSIMLPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGJ7</accession>
    <name>A0A1E3NGJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_73982</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016319.1">
        <property type="nucleotide sequence ID" value="XM_019164638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGJ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGJ7"/>
    <dbReference type="GeneID" id="30181325"/>
    <dbReference type="OrthoDB" id="3437016at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="33"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="541" mass="59029" checksum="0F3EF422C8FD4954" modified="2017-01-18" version="1">MTAASESSPLLPIADSPNESGAKKLSPTAFRIILGCLYANVFLASVDSTMVATLLGKMASDLDSQEHISWVATSYLLSCAAFQPLFGKISDVFGRKPVILFSTVCFFFGCLMSGVGDNLTYLIVGRMITGIGGGGFFTLATISVSDLVPTRERGLYQGYINIFFHAGSASGGVLAGIIDAYFGWKWAFLIQVPVCFITGLAVYLWFELPRKADANDDKTHWENFVSLDWTGAALLVTSLLSLMIISGTNGSELPVSSYRWKGLAAYTVVGFVAFYFWERKTDQPIIPVKMLHNQTILASSLNCWFTCMNMFSSLFYLPFYWTSVKNVSPLDCGYRLIPSSLLASFTSVFCGWMIKKTSRYRSLHILAGWCMFLGSGILYLSTKDDSSFRDSIISLPLRYGSASNITTILVAMISAVPQSEQALVTSIQYGFRSTGSTMGVSLASALMQYILRGTLDSNFDALKHVKLPDGWTAEMVKAAKLQALESPGYAFGDAIPAVIHNAIVSSYSTACHGVFLFLIGTGLASLICIYFTEENDLDKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGR3</accession>
    <name>A0A1E3NGR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_179119</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015646.1">
        <property type="nucleotide sequence ID" value="XM_019161802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGR3"/>
    <dbReference type="GeneID" id="30178489"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF39">
        <property type="entry name" value="TRANSPORTER LIZ1_SEO1, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G00980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="56564" checksum="F31A08FC87AB6430" modified="2017-01-18" version="1">MVSVVNKSEDHISFGTDVVTEIKGDDIEHVTTKVESFQESDSTEPVLQNTSWKRFIWDTWGHPDKKHKKIIFKLDSTLLFFACASTFVKYLDKINLTYAYVNGLKEDLHINGNQYNYANTGYNIASMICGFPAAFLMVQMNSRYFLILIELLWTIVTFCQTAVKTPTQLIVARTILGAFECGHYPALAYILGTYYSPLELAKRSVILQATTSIGSFFGGYVASGVYTGLNGVNGRAGWRWVFIIDGVISIGLILFQPDFILNEDDIHYLKKRLPEQQTDNYKFTYKDIISALTDWKVWGFWFFGVSQDITSLTNTFFSLWLKAWNTKKPHSYTIPQINHYASIIYAVQTFVALGGGWISDTLLKGNRWLPIAISGFVAFAINICLGATPLYPHNRAFRFFLYCNTGWALGTAGLYWAWAQEIFKDNVKKRVICTGGINIFSFVANSICNNIWYKTSKGPHITAGYYLSGTCGLLYSLNAFLLGFGDRRRLRDVRNSLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGZ3</accession>
    <name>A0A1E3NGZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016538.1">
        <property type="nucleotide sequence ID" value="XM_019161794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGZ3"/>
    <dbReference type="GeneID" id="30178481"/>
    <dbReference type="OrthoDB" id="5418899at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="35"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="578" mass="67367" checksum="97B70BA9E16B72CD" modified="2017-01-18" version="1">MARSKVEKSKETTSSLLSLPVFSSSLKGRKKSRNGCFMCKRRKKKCDEKKPVCSTCRRLNIQCSYKLRLSWQAVNQRFVEQESHFVNPESTTANGYHFIIFSIWDVLVSYKLRQPASSGTLYILEPFSLEKYENDTKTEEEWRYEVSKINSSVIPRSELERIQNDLTYGPRTSEQFLFNLYTKVLSRTKSFARSEFIPNDFIHIVIPGCQKFPALYRSVLALSALDLIKGELCKKNSPHDEYLITIYNSLFIGYKNDALNYLHDILDGFDIEVVEMLEELVITILILCNIEITNKGNKEWVRYLTEASLIFGALTTEKILDSDIFKFAYKYFSLRYILLLTTLDHITLKRFLDTTPWPLIESLFVDNTIEPMFGCSPYLLHVIYKLTIFSYLYEGKKMQMHEFVAKLTKMWYILNTITQDEIDGCHELTISASCYFYATKIYLYTILIRHKLEHFLDDGYQAYVPKLWDQLTELSLSQNSLFFPNWCFLIITTSDLLENEEAKRIKALRLFTSLEINWPLSSVVQIRKAIESIWKVYDLVYVGGSGGSGGKSPLDRDKPDPFDCRQVLKEYEYMLALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH08</accession>
    <name>A0A1E3NH08_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">arsenate reductase (glutathione/glutaredoxin)</fullName>
            <ecNumber evidence="3">1.20.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17377</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ArsC family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015961.1">
        <property type="nucleotide sequence ID" value="XM_019161577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH08"/>
    <dbReference type="GeneID" id="30178264"/>
    <dbReference type="OrthoDB" id="59229at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008794">
        <property type="term" value="F:arsenate reductase (glutaredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03034">
        <property type="entry name" value="ArsC_ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006659">
        <property type="entry name" value="Arsenate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00014">
        <property type="entry name" value="arsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041:SF5">
        <property type="entry name" value="ARSENATE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="141" mass="15999" checksum="C47941925FE3DEC8" modified="2017-01-18" version="1">MSQPIKIYHNPACGTSRNTLAMIRNSGQEPVIIEYLKTPPSREELIGLIQDSGLSVRDAMRKNVEPYDTLKLEESHWTDEELISSMLKNPILINRPFVVTELGTRLCRPSEVVLDILPTPQLSEFIKEDGEVIVDKKLNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH16</accession>
    <name>A0A1E3NH16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_74199</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016553.1">
        <property type="nucleotide sequence ID" value="XM_019164706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH16"/>
    <dbReference type="STRING" id="763406.A0A1E3NH16"/>
    <dbReference type="GeneID" id="30181393"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="11">
        <location>
            <begin position="38"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="459"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="460"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="479"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="518"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="551" mass="61856" checksum="C14C1F27A83A507A" modified="2017-01-18" version="1">MLYEDKPFGEIKVNPQDKKRIAYFYDSDVGNYSYGSAHPMKPHRIRMAHSLIMNYGLYKSMEIYRAKPATRQEMTQFHTDEYVDFLARVTPDNLEMFNKEQLKFNVGDDCPVFDGLFEYCSISGGGSMEGAARLNRGKCDVAINYAGGLHHAKKSEASGFCYVNDIVLGIIELLRYHPRVLYIDIDVHHGDGVEEAFYTTDRVMTVSFHKYGEFFPGTGELRDIGVGKGKYHSINVPLRDGIDDQTYKNLFEPVMKRVIDWYQPSAIVFQCGGDSLSGDRLGCFNLSMYGHANCINYIKSFGLPLMILGGGGYTMRNVSRTWTYETGLLNQKVLGPELPFNDYFEYYGPDYKLEVRPSNMHNANTPEYLNRIMTQIFTNLDNAKHAPSVQMNEMPKDILDFEDDVEEDGNEAIDTRGGSQKRRDEMTVPENEYFDHDEDAVKVKDKDIDIDMTDAKPAILSTSDSTPMTTSEPAFEQSTTTTAATTAVPTETTTAPAIAPVANVTQESVKSESKPEPENTTTITDTAAVTAAEGKKFEAKVPIEDKMDTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH66</accession>
    <name>A0A1E3NH66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS11m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_17421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016011.1">
        <property type="nucleotide sequence ID" value="XM_019161596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH66"/>
    <dbReference type="STRING" id="763406.A0A1E3NH66"/>
    <dbReference type="GeneID" id="30178283"/>
    <dbReference type="OrthoDB" id="1654884at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000011">
        <property type="entry name" value="37S ribosomal protein S18, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="202" mass="22626" checksum="E09C3BEB5C55DB33" modified="2017-01-18" version="1">MFGNFAQLARGSVRAFSTSGAAKNTITTFGEAAKNPSKLNRESKAATKKIKDIQTVKNVLHCNFKKNNTFLTLTKVLMDKNYPANHPGESFNEKVLYFLTLPQRIVISQSTGYLGFRKAQRGEYEASFQLSSHIFKNINEKRLLQDSGKLEIIVRGFGKGRKAFFDALKGKEGNGIREYISRLSDLTPIAFGGNRAPSRRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH98</accession>
    <name>A0A1E3NH98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_140510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016614.1">
        <property type="nucleotide sequence ID" value="XM_019160199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH98"/>
    <dbReference type="STRING" id="763406.A0A1E3NH98"/>
    <dbReference type="GeneID" id="30176886"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="7">
        <location>
            <begin position="481"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="599"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="604"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="879" mass="102437" checksum="C133B7F8B0400517" modified="2017-01-18" version="1">MHFPGVLRKIRRQPILISIFFLNILVFSLYFTIYQYGTFSTPLASFKGNNIKAYSSESKAPKKQAPSMSSLLDKLAYIKDDSIETMTNTLYRKLFSPEIANSNWRFVDHFVNSKIGLDIARTFSYVKLRKTQHLYDPRITMTVYLNYLHDEYKKGSNKIPFSWEDWTDLSVLNSYLGVENSTCYSFFHSHDIDLDYTFEANKSSLTYFDKTHCLDNEEYLATPDGEFKDPELLPGFNFNGQINEKSDFIGQLHNAKSYLLSAAPPPTLIYFLHDNGTYHKVEPYPSSSMMRNGMFDSFVRKNSFQGFDPIAELENLSPKRLLKHQSDFKSAMVSANSYELSIPESRFALNTDEMFHYLYAKKKTSLDTNEKKFLEAIDFSKATKVEDAPKYFHEVNVKWYAKYKGHEIKENGAHYDFRFFSGFITEMPSEEATVHSPEYNAATPVNYTLSIDNTINRQTIILSHMVHTLFTLTFHDGLFMFPAHGSLLAWYFTSMSFPYDEDEDVQMPIADLAEFCLRYNDSLIVQNPKYGMGKYYVDCSSSLTHRGKGLGSNNIDARVIDVDSGMYVDITGLSVSSDQLNYENLHKFEGWIPQQAIDSYPLEPKQKRELDSRDDEKKDEKEDEKKDEKKDEKKDEKDEKKDEEKEEPVHKPTDEEIYNIHKENKLYNCRNDHFYTLDQLSPARLTLFEGAPTFVVASNRSLLEVMETEYGKKSHGNPEWRNWLFVDLLRMWVNTDDIKSACDELRRATNTTKEDNVSPNECFNRNTTFILSELVKNSIYDVTLQDFLAYKDKKRPDFNLLQELYRDNPYTGAHVREMQQFHGKWEWSTPYIDDRANISTEWENLAGWMLEDHPPPKIAMLDYLLFTEQEDHGLNIELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHE5</accession>
    <name>A0A1E3NHE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative tyrosine-protein phosphatase OCA1</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_140906</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative tyrosine-protein phosphatase required for protection against superoxide stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016629.1">
        <property type="nucleotide sequence ID" value="XM_019160216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHE5"/>
    <dbReference type="STRING" id="763406.A0A1E3NHE5"/>
    <dbReference type="GeneID" id="30176903"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF70">
        <property type="entry name" value="PROTEIN OCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="144"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="144"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="427" mass="48060" checksum="2A613EF46CFD5B96" modified="2017-01-18" version="1">MLVPPENYSMVEDGIYRCSKLDAINISFLKSLELRSIVWINEEKPPRVLRQFIEEHSIRLFHMMNSSVLPEEDEITNLKYQEWMVLKPSLISKSIEIILDVTNQNCLVMDKSEVIVGILRHIQRWNYSSISNEYRLYASRASYKDKDKDKDKDKDKDTDDASRRLDDAPPHEPPPHMHPTQRRRGTPPHAPDPPADEPHNTRYDPSASHSSSKAISIQRTPSTHDPVHPLTSPALRRLSIDHSAGVLPTPSPPLSSSVGRLSISTSPQIPKNLLKIVEMRKNKKKHNMELREKEKEKEESRPEPLPLPHSSDLPPALSPPSPLKTLSTTAVDTYRWPPAPPSPFALPPASSSSASSTDGGPPATAPDCDWPAGHWKLYKPRVDGTHVRRHGHAGRPPRPVLTAIRVQLPPETCLPAWFLELRALCGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHG8</accession>
    <name>A0A1E3NHG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Leukotriene A(4) hydrolase</fullName>
            <shortName evidence="17">LTA-4 hydrolase</shortName>
            <ecNumber evidence="17">3.3.2.10</ecNumber>
            <ecNumber evidence="17">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_73090</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Aminopeptidase that preferentially cleaves di- and tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze the epoxide leukotriene LTA(4) but it forms preferentially 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than the cytokine leukotriene B(4) as the product compared to the homologous mammalian enzyme (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>an epoxide + H2O = an ethanediol</text>
            <dbReference type="Rhea" id="RHEA:19037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32955"/>
            <dbReference type="ChEBI" id="CHEBI:140594"/>
            <dbReference type="EC" id="3.3.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.10" evidence="17"/>
    <dbReference type="EC" id="3.4.11.-" evidence="17"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016685.1">
        <property type="nucleotide sequence ID" value="XM_019164377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHG8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHG8"/>
    <dbReference type="GeneID" id="30181064"/>
    <dbReference type="OrthoDB" id="79562at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061957">
        <property type="term" value="C:NVT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120113">
        <property type="term" value="P:cytoplasm to vacuole targeting by the NVT pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000009">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000004">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012777">
        <property type="entry name" value="LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02411">
        <property type="entry name" value="leuko_A4_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 17" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15 17" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="18">
        <location>
            <begin position="482"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="156"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="284"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="596"/>
            <end position="598"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ45572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="641" mass="72978" checksum="1476779DF718F77C" modified="2017-01-18" version="1">MAGKDHYPHISAKLREVLKPHLPASSPEADPSTLSNYEKFSVLHTVLSLDVLFKDKKLAGSISLSLKCLQQTSSIVLDTSYLSIHGISIDDKSVKDYKLKDRMEPLGSALEIRHSLAADDEVTLKIDYETTDRCTALQWLDPQQTNGKKLPYLFSQCEPIHSRSFFPCFDTPSIKSTFSYFLTSPFNTLTSGLLKDKKDSANNVVYHFEQPVPIPSYLVSIASGDITGAAIGPRSTVYSEPSFIDNCQYEFEHDTENFIKTAESIVFPYEWKDYDVLILPSSMPFGGMEHPNCTFATPTLISGDRENIDVIAHELAHSWSGNLVTNCSFEHFWLNEGWTVYLERRIQGAIHGEDFRHFSAIIGWNDLTNSIISMGNSAKRFSTLIQDLKDRTDPDDSFSTVPYEKGFSLLFHIEQTLGGKEAFDPFIKHYFNEYKYKSLDSYQFLDSLYSFYSDKSDLLDTIDWQTWLYEPGLPPKPSFNTKLVDECYTLAAKWVDIIETAPEKLSSKFKSTDIAKFSANQSGVFLDKLCSYEGQNGFTWKNENGKKAIDLMTNIYSKYSVSQNAEVIFRWFRLLLTANITSSYQDLANWLGTVGRMKFVRPSYTMLNKVDRPLALATFEKYEMIYHPICRSMVKKDLGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHJ5</accession>
    <name>A0A1E3NHJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_13473</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015926.1">
        <property type="nucleotide sequence ID" value="XM_019160064.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NHJ5"/>
    <dbReference type="GeneID" id="30176751"/>
    <dbReference type="OrthoDB" id="524326at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF170">
        <property type="entry name" value="DYNAMIN ASSOCIATED PROTEIN 160, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="172"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="328"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="1305"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="465"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="885"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="629"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="471"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="493"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="563"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="904"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="922"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="945"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="966"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1015"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1059"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1093"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1131"/>
            <end position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1347" mass="143968" checksum="D23F345644897BF8" modified="2017-01-18" version="1">MGALNGTLQEPLSAEEKKFYGSIFKTLDPKNTGLISGGVAKPLLEASGLSLPILGEIWNLSDSENAGYLNQFAFCVAMRLIAHAQKGDPITVTSANLAAPIAKFRTIQVNNTGSGILNNHLSAPLQSSQQHGNGVPPPQPRITSSNSVQSFASHSSTASNPSLVVPVLSSIQAANFGAMFDKTAVNGILPGAQAKNIFLKARLPIPILEQIWNLVDQKEAGQLTRPQFIIAMHLIQCFMNKSLTILPTVLSNQLWKVAGGNPASADTNRQPTNLQSVASPPPRTHSQVVPPPHPQHAQTAPLNQQLSGGNVVASPTTNLNTWIMSSQQKSQYGAIFDTLDTTHESSISSSTVANFLMTSKLPNQVLANIWELANLDQSDNFTKQEFSIAMYLVQKKLAGYELPEETPLELVQSSSFNEAPIHPSQFTQPPQSFASAKAMAAPQRTPSHMDDLLGIFQTASPVAAAPAPLSQGPSNGGQHLNHSQTLPPPAPSSRLSTNLTGSHFTPTTEFGRGLQKSVAEEDEESSDDDDGPENLDLPRVRGAPPAIPGRSNKPNFDSEVGRPTSNYDAIKQVSNDPTSMFSNQPASNGLGGFTNSAISQSASSVPTAATISGFSNSAPATDRAFTDQITQASVDIANYSNQVASLSRQTSVVSGKKDKAQNELNKILKKKEEILGKLSQLKALHEKENQQVYEVQNVMIKSKAELDELEKELSINEANYHAEQTKKEQLQAEYEESQKNNQMLKEQLGTLNAENIDLRNQIEDLSGKVKQSNNMLAVTQQQVATQDAQNEELRQRVSELNNTIFQIHSKHESLLHKIAGLNDENLGLHEQHTDLSIQSANKNVEYSEALAEAANKGVIGDDFEAEDANGEIPTASLDDFDEEFGVESKASQSEQLKTKAPDSPGTTTDTVMSKQTDTTNPTQTASDSSATTTGQQFSLPFGTPHSETSSTRNNPSQSERGDLDASPTSTIEQTGPLSSQILPEGTVSEAGDSASSQEKSESFEMINHPVDTSKVSPDSPSVDTTPSETNKELPVAASSSMAAAVTAAGFTGPVRYPSASGQTSEVETLSDTPHDHPNQTPELDEFPPIQELEPLDDGDSSSSDEDNFQDAHVSSDLQLAKGVAVTEDSKNPFNKQQNGSDEMQPPVNAATGPSMFDDLGLEEAQVEDSADAFDSPEMSGGAFSNIGFTYAGNDPNTGLANATTKTVESADKVAPAIAKSSKSKAVAADSDDWEQVFAGFGNDPHLQPASVEEGNTSYDSHFQQPAVFGFSAANEGADGNFSNGLSQIVDSDIAKAKGEFHSKFSEAQQLAVEELSDMGFESQDAIAALKLKNWNIDDASNYLLDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHR1</accession>
    <name>A0A1E3NHR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Diphthamide biosynthesis protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_25899</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DPH4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016803.1">
        <property type="nucleotide sequence ID" value="XM_019162419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHR1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHR1"/>
    <dbReference type="GeneID" id="30179106"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050817">
        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="5"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="DPH-type MB" evidence="7">
        <location>
            <begin position="96"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="187" mass="21154" checksum="ED7DD14AB441C8CE" modified="2017-01-18" version="1" fragment="single">LNHISYYEVLGVPQDCSTKDVKSAYHRLLLTHHPDKKVQREDTTILPNRHSIQLIQDAYRTLSDTQQRKDYDAAIHTHFIKLGLTALSASTVNIDGMDRIDLSEFEVTESQPEKGGVGTAGDSELEEVFVHACPRCHFEDGFVLSETDLEQGTHEQEQNRLNSTDVDYQLLLQCASCSLWLCVTYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI29</accession>
    <name>A0A1E3NI29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17048</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016887.1">
        <property type="nucleotide sequence ID" value="XM_019161441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI29"/>
    <dbReference type="STRING" id="763406.A0A1E3NI29"/>
    <dbReference type="GeneID" id="30178128"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097435">
        <property type="term" value="P:supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000009">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="446" mass="49781" checksum="D02D900C29B757DD" modified="2017-01-18" version="1">MREIIHLSTGQCGNQVGAAFWETICNEHGLDPNGNYHGTSPLQKARLNVYFNEASSGKYVPRAVLVDLEPATIEAVKSSKYGNLFRPDNLIYGQTSAGNNWAKGHYTEGAELVDSVMEVVRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVMPSPKVSDTVVEPYNATLSVHQLVENADETFCIDNEALYDICVRTLKIPQPSYDHLNNLVSSVMSGITTSLRFPGQLNSDLRKLAVNLVPFPRLHFFMVSYAPLTAEGSQSFRSLTVPELTQQMFDPKNMMAASDPRKGRYLTAAAFFRGNVSVKEVEDEMHKVQTKNQDYFVEWIPNNVQSSVCSVPPIGLDMSATFIGNSTSIQGLFRRVGRQFTAMFKKKAFLHWYTSEGMDEMEFTEAQSNMNDLVSEYQQYQDASGLDDDLDYDEQNQMDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI64</accession>
    <name>A0A1E3NI64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73279</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016889.1">
        <property type="nucleotide sequence ID" value="XM_019164432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI64"/>
    <dbReference type="STRING" id="763406.A0A1E3NI64"/>
    <dbReference type="GeneID" id="30181119"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="85"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="205"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="313"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="245"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="614" mass="69198" checksum="3DE060394CB22F7A" modified="2017-01-18" version="1">MHLFLHSSEKSSRKRTQSFSSQRTVSSASSSYSFSSSPQTQHTEPAADLIFKQPNRDELLKVCKTNDPGKLNNLDIPYLSEYILMDTIESLDEGSSGSILKAKSRTNRKSYVVKLLKSEVMMGKSKESSSISSSHISTTDFHGSNLSGSASLSRTHSHVNFERSGSSITTTTATPTNIRSPQPTETPAFKNQRYSPILSAAYRSPSRLVSPSSSKLSSSMQSLSLDSTRSRSNSSFKTFKPMNKQGEDENKKGREQGKDGEDYEGEENEEAKPILRPMYTFDSLNEYLILASLKSKHVSSVHGLFRYGVEHNDDAEADEDDDGEDDDGSFYSMDGNDINDNVKSSDEQNPVELNSEPIDVCLLLDYYSNSDLLRLTTGIRKKNISTSSLFKDAIFAQLVQGLKYLHQQGIVHRDIKPENILIDEEGCLKYADFGYAVDLNRIEDYPIDEHYFLNRGTTSFKAPEIMKVREKFDADMLKATDVWSLAVLYYQMKFLNKPWRCAVDDDCDYKRYRDLFTAKKLDSLKTGFDIKRVFGSEDVMGRSLKSVKDDVVIVVTNMMNPNPHKRWTVSDVFRSDWMVGTRMLIEEEDKASSAAGVRGKNSEDLELVKVLRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIA4</accession>
    <name>A0A1E3NIA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein kinase TEL1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATM homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16 17">DNA-damage checkpoint kinase TEL1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Serine/threonine-protein kinase tel1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Telomere length regulation protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PICMEDRAFT_34736</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016980.1">
        <property type="nucleotide sequence ID" value="XM_019162904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIA4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIA4"/>
    <dbReference type="GeneID" id="30179591"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="20">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="21">
        <location>
            <begin position="309"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="22">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ODQ45867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="341" mass="38792" checksum="ADDF8EA506E589AA" modified="2017-01-18" version="1" fragment="single">GVSHPKIMKMILSTGESQRMLLKAPDDLRQDSIMKQVFEKVNKLLWRNIETRKRNLRIRTYNVSPLGPTSGVLEFVPNSMPLIDILKSLHQGDEMDITEARLKMKEFQNQSKNVRIQVYKEICHKVTPNLRTFFFNNFTSSDSWFESRTLYCHGIATTSITGYILGIGDRHCNNILLDKSSGEPIHIDFGVAFDQGQALPIPETVPFRLTRDIVDGMGVTGVNGMFSKNCEHVLNVLRSNTQYISGILDVLKYDPLYTWTMSPLRKKKLKQIYFNNDESDKGFDEFIKTDTGSEANAAIETVKRKLGAQGLSNEAVVRELIHEAVDPRNLALIFMGWSPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIG0</accession>
    <name>A0A1E3NIG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017048.1">
        <property type="nucleotide sequence ID" value="XM_019164474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIG0"/>
    <dbReference type="STRING" id="763406.A0A1E3NIG0"/>
    <dbReference type="GeneID" id="30181161"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000237">
        <property type="entry name" value="Pumilio homolog 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF13">
        <property type="entry name" value="PUMILIO HOMOLOGY DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="507"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="530"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="566"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="602"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="639"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="711"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="799"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="172"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="463"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="880"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="182"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="887"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="928"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1095" mass="120359" checksum="A751625A2B8F1442" modified="2017-01-18" version="1">MSDQTTLSSFDDSISQININATTTTTPPMGEFSKQPNQSQMSDTILNTNSASILSNAANINDQLSGRDFNSLPGVLSSLNLDDNPVFSPKVKNAGNHPSTPPSDWKVANAWSNANTSNFGGFSTIPEFSSLSAGNLNTAVNNNSHQHSSSGSTASYTRDDTTKEGVMHINNQSSPFNPSQIGAASKNTGTAATSATTNTTNAEIHNNNNNDNNGTSSTNAANINNNTNNHPITSRNSSFIMPDNQLDSYLFEHSITNTPSSSSILSPNKHRNQTFSTPILGETSRRLNYNLSSPNNQPSQLNDFNDNNDTSIGDLSQTQVSSALHPALTNDILSGTVAGESLGMGITPTMSGPEQGQINPSFFGNYIPNQFGFLQSSGPSAKVPSMNGLQNNMMNPGSPALNLLPNQININTNPQIRPVFLNSPSMNGNGQQNFLAQNYPYVDKLNSRPFQDMNKNVMNKDLTNNNPNPKDPGFGVNNNGTLKTNNISNNSANHRNNLGLNSTANIHRKHTSNNKRGEDASRFVNATLSDFANEIFYLCKDQHGCRFLQRQLELNGDVAATKIFNEIQLNVIELMIDPFGNYLIQKLLERVDETQRTTLVRNASTQFVRIALDPHGTRALQKLVECISTEAEFQIIINSLSSYVVLLSRDLNGNHVIQKCLQKLPPSYCNFIFDAACENCVKIAKHRHGCCVLQRCFDHGTPVQCEQLALKVGENAVELSIDPYGNYVVQYVLSMEENRLRIQNDPQSINKIPDTSKSIDFIIAALASNLTKLSTHKFGSNVIEKSLRIPTLAPLLIQELLQNPESLKLLLHDAYGNYVLQTSLDVADDSSFKELSELLKPYLNDVRNTPHGRRILSKLISRGGNAAVFNSGLLNGEPFDNFDASQSNNNSMNLSNNNNNSNNNNNNHGRRLSHMHNHGHGHGHNHSSQHNNNSNNNHVFNSGYNMNNRVMAMDPPIIAENNDTNHSHNISLAFLAELTSALRLNPESHIEIQTFTRSVTSYTAIFNTTEFRLCLGNISSHDTKIFGVSASDVPAIQVAKPLGLSEEENRCVFSCLKQQEIDYLGFFSGEVDLLRIEEDFHELNDELITWRGSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIN9</accession>
    <name>A0A1E3NIN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73477</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017112.1">
        <property type="nucleotide sequence ID" value="XM_019164489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NIN9"/>
    <dbReference type="GeneID" id="30181176"/>
    <dbReference type="OrthoDB" id="27823at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120104">
        <property type="term" value="C:mitotic actomyosin contractile ring, proximal layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="8"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="604"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="361"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="455"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="76969" checksum="AE4FC62A91F4964A" modified="2017-01-18" version="1">MATAIPAEQLYARSFWSVDSSPQEVLMLQNEKATKTLNSLSSFYKEFMHLENDYSRRFNTLLNRLELPKHENAGTLKTSINVFQDQCLRVSESHSLQARRIQDALQQPLLELVSDRKAREKVVETKIRQSWNELTELRNKCGSKSDKYEDLWSSMSSLKRTRMTLDNIEAEKLEQKLSAMKTKMLIIREENWELVNKYNEKLDAWLTLWWDTCNEWQAAEEKRIRFLKSNLWEFANILSLFCVEEDQFAENIRVSLQDCSARKDINYFVDENSTGHELLAPLKFVDFAKNETRPVHEQISKKFDILEIPSIREKVTEEKEIRQKRRNPPPKSTEQGETAFSFIGKSKETFNKLQEEAQKEVSQMKINKPDPNDAKSISEPSTYQTMSDYSNPTTRTSISSHSFTDSLNDQILHYKKINSDSNKDKDDNYELGGLKDDNNPIRNALINLRNEDKMRESMSPLKEDATKKEKYLRESMSPAKQNSTKKDKHNSFANIMKNAFNESPGMDMASKNSKKFEFRQLLPNTATGQRHLDTETSTNDNNQTDAMRIISHNSLMIKNQKNSLLRPSQRQISSSMRKSKSQMNLKNRHISLSELPSHSSEGYPVISYTKAQYNYTAAIEEELSFKKRDILLVLHKQSDGWWFAENLNSSDSGLVPSNYLVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIV2</accession>
    <name>A0A1E3NIV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_12503</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017178.1">
        <property type="nucleotide sequence ID" value="XM_019159937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NIV2"/>
    <dbReference type="GeneID" id="30176624"/>
    <dbReference type="OrthoDB" id="21266at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070692">
        <property type="term" value="C:CTDK-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042326">
        <property type="entry name" value="Ctk3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024637">
        <property type="entry name" value="Ctk3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024638">
        <property type="entry name" value="Ctk3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28291">
        <property type="entry name" value="CTD KINASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28291:SF1">
        <property type="entry name" value="CTD KINASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12243">
        <property type="entry name" value="CTK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12350">
        <property type="entry name" value="CTK3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTD kinase subunit gamma Ctk3 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="CTD kinase subunit gamma Ctk3 C-terminal" evidence="3">
        <location>
            <begin position="330"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="58089" checksum="2189AC436313AC64" modified="2017-01-18" version="1">MDSFELQMQLTGLLMGLNSSKQASFEICNFLIRNYVTQEDLYPALLDVLPKLDINKRLNFFQFIDDFLTLVTTEPKYKDNDIIFNYAFLIISDLHKILQYVLPETPSNVTDSSIAGAEAGVPKRSDIRTLANLPFCYSMLVHISKLFNLQVLAQFQKKYNSNLLTETDIDNVKRGLYFDEKSMYMENVEPTKSSHVDDYVNSSPKATDDSHQQQLEDNYIPEKINQGLIAAWDFIIKKRKQSEYEFLLIDYLDDPFHIKPMESTSKIPNSGANINNTSSPTIFNTSVKNTEDGATAKTPAKTPGKTPAKTPGNVSSQTPSNSNILSLTQSLILQRIEADRERQKRGKETLWEVERPSGTIKLAEFEYIYDTLQPYDEVGDKPIIDEMENLYELCTFREAGITKAANKSHPPPTKQKPSNRSSSTKAKSRGKTAYDSQDLFYSNHKTKRPRTHPNMSSKNTYIPNSNSNEYMDSRLYDGYYDYDSEWYYKNGRQSNSKRGGYGYERKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ62</accession>
    <name>A0A1E3NJ62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017297.1">
        <property type="nucleotide sequence ID" value="XM_019164141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ62"/>
    <dbReference type="GeneID" id="30180828"/>
    <dbReference type="OrthoDB" id="4056983at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18785">
        <property type="entry name" value="SF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26970" checksum="BC4AD6DF422B5395" modified="2017-01-18" version="1">MGTKLISNGIDVSNVNLVVFSGYSPQDIEFIQGLGRIRGQGLCVVLNHNQFCGANIMGRFYGFKDKDHFWCCGRPEDEELLAMFDSVIGDEDNRSKVHEDSAILNSSFRVEDLGLDLNFALFEVTSTPCKNGGGEKRSLDEDIDLENLSEMYHEMFSEYPELNTCEKEGLTSPIGYLVLIHLLLVQIIKFSRPCLNEAASCLAGRLIQSSNIFVFLAPTLVADDCMDVFGFGVYKYIYILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ99</accession>
    <name>A0A1E3NJ99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_66820</name>
        <name evidence="11" type="ORF">PICMEDRAFT_73617</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017259.1">
        <property type="nucleotide sequence ID" value="XM_019164537.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018185.1">
        <property type="nucleotide sequence ID" value="XM_019163493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ99"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ99"/>
    <dbReference type="GeneID" id="30180180"/>
    <dbReference type="GeneID" id="30181224"/>
    <dbReference type="OrthoDB" id="3969558at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000211">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="5"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="459" mass="49946" checksum="A7BC265E5E0E6BA3" modified="2017-01-18" version="1">MGKDKQHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSVKWDESRFEEIIKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEPSTNCPWYKGWTKETKASGVVKGKTLLEAIDAIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPTGVSTEVKSVEMHHEQLEEGVPGDNVGFNVKNVSVKDIKRGNVCGDSKNDPPMGCASFNAQVIVLNHPGQISAGYSPVLDCHTAHIACKFDELIEKIDRRTGKSTEDHPKSVKSGDAAIVKMVPTKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVEKVDKAGKVTKSAAKAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJC6</accession>
    <name>A0A1E3NJC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_73191</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016791.1">
        <property type="nucleotide sequence ID" value="XM_019164408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJC6"/>
    <dbReference type="STRING" id="763406.A0A1E3NJC6"/>
    <dbReference type="GeneID" id="30181095"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005621">
        <property type="term" value="C:cellular bud scar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005937">
        <property type="term" value="C:mating projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032160">
        <property type="term" value="C:septin filament array"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000162">
        <property type="entry name" value="septin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="9">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="356" mass="41802" checksum="00EB92D54F5E4A47" modified="2017-01-18" version="1">MLAGESGLGKTTFVNTLFQTSLKNHQELRNRYNKPIKKTVEIDIIRAELEEKNFKLNLNIIDTPGFGDNIDNKNSWAPIVDFIDDQHHSFMKQETQPSRGGVKDLRVHACLYFIRPTGHTLKPLDIEIMKQLGMRVNLIPVISKADTLTPNEMLEFKKRIRDIIEVQDIRIYSPPMELDDQAASEHAKQLIESMPFSIIGSEEEFEVNNQLVIGRKYPWGFVEVENDQHCDFRKLRSLLLRTNMLDLILSTNEIHYETFRAKMMNNDSDNYENRKGADKVSHKDTNNPQFIEEEKKLKRYFAEQLRNEDQRFKQWKQNIYTEKNRLNHDLAQLQAQMKKLEEEVRVLQEKKVKSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJL6</accession>
    <name>A0A1E3NJL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spt20-like SEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017448.1">
        <property type="nucleotide sequence ID" value="XM_019159781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJL6"/>
    <dbReference type="STRING" id="763406.A0A1E3NJL6"/>
    <dbReference type="GeneID" id="30176468"/>
    <dbReference type="OrthoDB" id="1932706at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="2">
        <location>
            <begin position="513"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="887"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="908"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="954"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="965"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="974" mass="107214" checksum="ADFEEF4CDF2DDE61" modified="2017-01-18" version="1">MSTGGEPNNLGFDQNGQTNGLGSFMDPVNQMGMAQSPNQPSPVDHMGQLNQLNMNPMSQMNSISQGNQMGQMSQLNQMNQINHLAPAGVQMNLNAIGQINNQHQLGQMGQRGHVGQMNQMNNASQMGHMGQASPQQRQQGSEVRGWNPAYVQSLLRKIQPGPGMTKQQRFQQLLRSGELTRTDVQILQRHQQMLRQAQAVRQKQLHQRALQQQQQQQQTSSYSGKSIPGKSLPGKKVPGKSVPGKSLPGKSLPGKSLPGKSLPGKSLPGKSLPGKSLPGKTVSGKTVPGKSLPGTGTPEFSPSLSHASLGGKSLPVNSTSQRTSGTETPMQISTGMSLPVNSPLAANLISASPLNNINSPVNPAVNLSVNQPVNSPVNLPLNQHINQPVNRPVNQPVNEPVNKPVNQSVNQYMSQMSNAASLVQQQAGIPQGNQVNGSVNPSQAHLEQLKQQEHQKKLLQQRQQQKYFNLTAEQYDKLNVQQKQLLKLQYLRRKAKTQRFYESDQELLKKYENSPPSLDFHIHEEHYRFGHNENLIPKNDPAIKDFLTYVAKKKIPDALMEIIKDGNIQLYDGNIILRIYDHRIVDDASLLPPKEPTPVPSSESKDLGAMVPNINPSVPAANDSLENSSNQSPDKINANASTTSTEIPESKNEKKAPKRFKEYRTILRLTQSALYEDFCLTTDTQMFGDSFVLTYESEILTAITRSVNLQPTNNPYLQGRSLWPTSEMVFPHYDEKEDRMIFTHRQDMRELVKSKLNHKKRALTYENYDYKPLHQDGSQSNSKYEKLMLIMGKSSPHSNISASKQSVEVPRFERLRFIENLRHQNLIKKENNLRGVSSIQSPPVGYTSFGSVMGNINNQKSFSFQGKNADNSTMADGEVKSEDNGGNSTSAANSKGKAGKASAGKNSTPRKKNDKPKKPRKPTKKQLAAQAAAQAATEGIPDGTSATSASEPPKKKRAPAKKKNAANTPVTPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK73</accession>
    <name>A0A1E3NK73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_16365</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017599.1">
        <property type="nucleotide sequence ID" value="XM_019161181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK73"/>
    <dbReference type="STRING" id="763406.A0A1E3NK73"/>
    <dbReference type="GeneID" id="30177868"/>
    <dbReference type="OrthoDB" id="1744952at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033679">
        <property type="term" value="F:3'-5' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="443"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="759"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="759"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="983" mass="109465" checksum="BED9BD4FA2A3BC59" modified="2017-01-18" version="1">MSNVASSPLLSQGPAVGAPSSQLSDFNLDDDTMNPQTNNTQSSFFGGGPSSDAPTGARGVRAEQSDLATGGSDTAHRIRVNAGVSNIPKVEDKISETVQESFEEFLENFKVDSDDDAYDPHFEGKLYLERVAKMVNDDSTLLNIDFKHLQSYQNGILAAAITEQFYRFLPFLQNGLRHIVSQHQPQFLRRQVSSAASDEASKTIEHNFQISFYNLTVVHRIRELKAENIGMLMAISGTVTRTSEIRPELYKAAFTCDICKALITNIEQTFRYTEPTSCINSTCVNQSEWTLNISKSQFLDWQKVRVQENSNEIPNGSMPRTLDIVLRGELVDRAKPGDKCKFTGTEIVIPDVSQYQLPGVRPATVRSRGAGPADSLTGGISMRSLGARDLTYKIAFLGCHVNSSSGKELEEAEDEGTSLENFLSNLTPSEVNELRDMVSDRNVYSKLVQSICPTVYGHEVVKKGIILQLLGGVHKKTVEGINLRGDINVCIVGDPSCAKSQFLKWVNSFAPRAVYTSGKASTAAGLTATVVKDEESGEFTIEAGALMLADNGICCIDEFDKMDIADQVAIHEAMEQQTISIAKAGIHATLNARTSILAAANPIGGRYNQKVGLRSNLSMTGPILSRFDLFFVILDECNEKVDTQLATHIVNLHLFRDEALNPPYSKEQVLRYIKYAKNVKPKLTPEARAYLIERYKELRIDDSQGGRSSYRITVRQLESLIRLSEAIARANCKDDVTPTFVAEAYDLLKQSIIRVEKEDLDLDDDQDDISGGDDDNNDQGDNGDDDGLGEGNMNNEDEDGREQMLEQSQGAKRPKGKRSNNIPYSVFINLRNVFLERIKDKEADSAIVAEEEGTHDEGPSGYTQKALLDWYMNAIEDQLESVSEYWDELKIAKKVLNKLVKDNILMRVNPDPTDFDENEIPADLVITPNMSVEEKERLTNEQNNWRRRQKRELNKKLVYIIHPNCSIEDLLGEVDEEVEVQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKL5</accession>
    <name>A0A1E3NKL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-rRNA-processing protein RIX1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTI2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017808.1">
        <property type="nucleotide sequence ID" value="XM_019161245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKL5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKL5"/>
    <dbReference type="GeneID" id="30177932"/>
    <dbReference type="OrthoDB" id="3997445at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018870">
        <property type="entry name" value="Tti2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10521">
        <property type="entry name" value="Tti2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="48688" checksum="5A815BCE9AA10C09" modified="2017-01-18" version="1">MPLPGAPLHMTTLNQVQLDELHLLEKKLVRKWVFWEEEDDITVIAEQNEIRKQCDSIVEQIDQCIDNNHASEKLVLFMGRFYLEDKSLAPWTSTKSKNISTRFFQRIVADANMKEKCESFIVDKIHNTLQEMKSANLSSEVNSSGYKKTSKLKIGGKLIGSTYTKMLDKMEKFKNDHLTELGHLHFLIEKTDMEKNWRYILPLLLALLDDTDVLVKREAALLLDMICLKLAIIEPIPANIIIKSQTMPLFKTAIQPLLLALPSLTPETKSVEILLPAYKAIFDLFQVSITDKLEFYNSMSALLNDTLLPSIGKCKDYAQVSLELTLILQEFLQRCGDFSKVLTKQVIYTLLTVLMDPYISFAPAVVSAILLVIQECMASNSAESRKRFKYDVLGCMGILKRRLQNRENHLDANIEGQIEVLVNCVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKQ7</accession>
    <name>A0A1E3NKQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_11700</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017837.1">
        <property type="nucleotide sequence ID" value="XM_019159827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKQ7"/>
    <dbReference type="GeneID" id="30176514"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000003">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="125"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="239" mass="26900" checksum="34C4DA812266DC61" modified="2017-01-18" version="1">MPRSKRSKLVTLAQTDRKGKENKVRIFDEIREGLDKFRYTYVLGLDDVRTPVLQDIRKDWAGSKLVLGKKKVLQKALGQTPEEEYKNELHKLARFDDGLIGLLLTDESPETVAQYFASYEKKDYARAKTRSPITFALPQGVVYSRGGQIPVEEDVPMSHSMEPTLRNKFKLPTKMVQGKITLTEPVTVVEQGHTLDVTQALIMKTFGVACAAFKVKIYAFHDGDTSDVVVLLDPETANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKS0</accession>
    <name>A0A1E3NKS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_33204</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017832.1">
        <property type="nucleotide sequence ID" value="XM_019162806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKS0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKS0"/>
    <dbReference type="GeneID" id="30179493"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF22">
        <property type="entry name" value="GLYCOSIDASE CRH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133404" description="GH16 domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="5">
        <location>
            <begin position="61"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="345" mass="37807" checksum="105D252AE38B5596" modified="2017-01-18" version="1" fragment="single">MKFTLVQTLAATLFAAAAVADTLSCSADSHCPEDFPCCSNEGTCGTGSYCLGPCDPRYSFNSSSCMPAPACKAGTYTPRAADMMNQTDYLGNTSVTDFIYYGEIEDQDDSVIIKMPAHSVGGVISSTFYLWYGKVSFKFKTSHNAGVVSAAILFSQVQDEIDYEFVGSELEVAETNFYFEGTQNYTNSVKAAASDTYANWHTYEIDWSEDSVTWSIDGVSVRTLNKADTYNDTSKEYDFPQTPSRLQFSIWPAGDPTKNAPGTVEWAGGDIDWNAKDFSDPGYLFVALDTIDVVCYDPPSDAQVKGNTSYIYDGNGFLQSNVIVSDKETWMKDLGRTGFNTGQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKU6</accession>
    <name>A0A1E3NKU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_58993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017877.1">
        <property type="nucleotide sequence ID" value="XM_019163334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKU6"/>
    <dbReference type="GeneID" id="30180021"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="68" mass="7591" checksum="AF90A229C561BD1B" modified="2017-01-18" version="1">MKQALLDLAVTQGIWRFTQHSSESPSNAPARKSDSALCANVMQHNPKDLQAEIAKCEHRVFPRICSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKV2</accession>
    <name>A0A1E3NKV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72819</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017895.1">
        <property type="nucleotide sequence ID" value="XM_019164302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NKV2"/>
    <dbReference type="GeneID" id="30180989"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23822">
        <property type="entry name" value="RWD_ScYIH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF25">
        <property type="entry name" value="PROTEIN IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="9"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="36810" checksum="6E1BD8263472B919" modified="2017-01-18" version="1">MSESKDLELEVGAIEAIYPECIEKLALCIYKFKVPQHEHIIFQMSFPEDYPEAKPHVLDVISEKEGFDENYLMELFQEVLDSIYVAGNVVIFDLFTELDAVLYTGEEDQEVLADTDHWNTEDTDLGLISEQLAASAFEDTSKEGYLNYVYDEKLEKAKMTTPKKNQTVDNKNGSSILKQRNTLDGWFISDPISDRKSTFVAFAIKVHTVDEAYRRFHELMEDKKCGRANHAMRAWRIKDEQTGARYQDCDDDGETAAGGRMLHLMTIMDAWDVMVVCCRWFGGVHIGPDRFKHINSATRDVLLKGSFVQLESSSGKKSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW2</accession>
    <name>A0A1E3NKW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017905.1">
        <property type="nucleotide sequence ID" value="XM_019161279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKW2"/>
    <dbReference type="GeneID" id="30177966"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="90" mass="9246" checksum="5E464D17544EA1CC" modified="2017-01-18" version="1">MPGMPGMPGMPGMPGMPGMLGMPGVPGMNGMNGMNGLSGMNGVNGMNGMNGMNGMNVVNNQNMLNMNGVNAVNNFSNGRSLPRSRPQTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW4</accession>
    <name>A0A1E3NKW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Delta(14)-sterol reductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Sterol C14-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_33309</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP(+) = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:18364"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.70"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:18563"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017873.1">
        <property type="nucleotide sequence ID" value="XM_019162811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKW4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKW4"/>
    <dbReference type="GeneID" id="30179498"/>
    <dbReference type="OrthoDB" id="10262235at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000009">
        <property type="entry name" value="C-14 sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="9 16" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="441" mass="50681" checksum="0586872BB567553B" modified="2017-01-18" version="1">MSQLNPVTINKDFGGAIGASTLTVFLPLVVLFLYLACNDQYIAEGVKIDASKVAAIFKEQVLNDWKNVFFNSECWVFYLSWFFTLVGLDIFIPGKVISGVQLRDGTKLKYTINGFQLVGFLLVVLSSRVYLYGFHLPELDFLYDNFLGMMATSWIFSILLSFFVYFSSFLNLSGNQKNGKGTKERILSVGGNSGNFLFDWFIGRELNPRIGEWDIKLFCELRPGLLLWSLLNLASLQRQYQRLGYVTNSMVLVNVLQFIYVIDGVLNEEGCLTMMDITTDGFGFMLAFGDLCWVPFCYCLQSRYLSLVEINLSSIAVSIVIGVMITGFYIFKASNIQKNKFRQGKLPHLRSIQTDRGTKLLIEGWWGVSQHINYFGDMLVALSWCLPTGFNTILTYFYVIYFSALLLHRQTRDEEKCKNKYGKYWTEYERHVPYKIIPYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL20</accession>
    <name>A0A1E3NL20_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_25049</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer with PAM16. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017947.1">
        <property type="nucleotide sequence ID" value="XM_019162378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL20"/>
    <dbReference type="STRING" id="763406.A0A1E3NL20"/>
    <dbReference type="GeneID" id="30179065"/>
    <dbReference type="OrthoDB" id="240298at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF28">
        <property type="entry name" value="GEO10507P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7406" checksum="E838DAADA42F5E25" modified="2017-01-18" version="1" fragment="single">FVKGGFDPKMNAREALRILELKEASLSLKKLKDNHRRIMLLNHPDKGGSPYLATKINEAKDFLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL64</accession>
    <name>A0A1E3NL64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017987.1">
        <property type="nucleotide sequence ID" value="XM_019161307.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NL64"/>
    <dbReference type="GeneID" id="30177994"/>
    <dbReference type="OrthoDB" id="10258608at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056604">
        <property type="entry name" value="GBF1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23325">
        <property type="entry name" value="TPR_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="627"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="757"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1572" mass="177523" checksum="B54BC38C4621B51D" modified="2017-01-18" version="1">MLQTSTAFADASVARAPHPWQAADTSITTATTITDPLAFIIQECTFILSAMRKNNKSASSPFAAILLNQFLSDGTRADDNDLSSSSFGTSTVRNPNTNKYATKFKDDDHFLSGFVELRSILSDTRDITLVDIITLLQPFLVVIKSPSTSCHITNLAISSLAKFLHYGIISANQTNIVQALSQIVSTLAHCRFEGADQTQDDLLLVKIIQLLESIVASPLGDLLTDDSMYEIISTCFSLAINTRRRDMLRSAAETSLLTITQILFSKLKELESKPEIDHNTKSAQVEFTNDSLPIDTIGGSSSASPSDISKDRSTSEQSQSEENKAAKLIVSPLATTTASAENSVSTEDDELNTLASLDLPDKDADLSPYGIACMREFLNHTIDILLPDNKYRFTEATRTLALEILIRTIEVTGTHITKHNQLFSLISDNCCHHLVEIIQNSESSILVTLSLKLILYFALYFPNYLKIQLELIFVTIFQSIIADESMLNEDLSKYRETVFDMSRKGKFETNSLTEVEVAELRKEFDTGKSPQSKEFLIESLSVLWCRSPYLFINLFKSYDCDFDRTDLTSSLIKLLCRLSYSDCSLLTTGNVPPICLDGLLSFVNGIYERIKIGINENVDLSNGDKNPLIQQYTKKSDFIECVKKWNEKPEKGLESLQEKGFITDPNDDKEVAKFLLTNSGRIDKKQLGELLAKPAKVNLLKEFVGLLDFKNLRPDESLRVLLNYFRLPGEAQQIDRIVSTFNERYIECQDPIDEVDEKALSEKTKNKDKDNGEEDEAVEEEEKVVPDSDAMYVLSFSIIMLNTDLHNPNVKKPMTLEDYQRNLRGCFKGKDFPVWYTEKMYQSIKEKEIIMPEEHKGTVKWFETVWQSLVAEQENKKVTDSDSINDTWTGSNNTAITDLLAFDKLLFEKHCQDLISSFIVMFDDATHDSIVTKMMSTVEKCAAIAIYFGIDDIVDQIVEMTAHLSTLTGVKKSEYLVDNRDILPIIEMDTNVREGEDTVFVSDLAVLFGRDYRAQLSMIILFRIMKRSKYKVTKGWFYAIKAVLKLFEIGLINPNIFEEFQKKLEFEKLNKPPAEFNIKKSEVLKESGLFSTFSSYLKGLSDDTPEPTQEEIEVSLAAIEFIESTGVNNLFSYVSKTRDFDSMNKLVQILLALLPHRNESTERFYVEEELFLLELCVCYLLVTENQKLINQVFSKCDLFLSEEKKDLKMSTVSRVLSYKLLLVHVSSDASKSVLDSALDKIHELAINSREAFVKHGVNVLQPLEQLAIVEDSWCCKDVVMKNTYWVILRVFASSPKNTEAVYQFISSIIKEHPTLIEYDNYMDILGLLDEISAVGAYGAQWEHEYDKLIDSGLKVEVNKNPYQELVNIALQSITLTSQLGDVINGTSFAKSTEEKKASGAEVVNPWYPLIEAISHQCYNPCRQLRNHALNTLTNLLITNPKLPLKELSLEKMLDAACLRLLVELMKPEVNGTDVKGMIKTQRDVLDLSCKMILTFDFSNVEAVVDKVFAVSSQLLQKNRASYPNSGHEDEVIELLRNVLMIKKDKLDLVKLKGYKMDGALKKLVEEVIKSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL97</accession>
    <name>A0A1E3NL97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Protein PNS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_72922</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Probably involved in transport through the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cell membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018003.1">
        <property type="nucleotide sequence ID" value="XM_019164333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL97"/>
    <dbReference type="GeneID" id="30181020"/>
    <dbReference type="OrthoDB" id="44736at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF4">
        <property type="entry name" value="PROTEIN PNS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="536" mass="58857" checksum="9CFC35C48913175F" modified="2017-01-18" version="1">MTSVDAKLQPVEAAPTDSVYRDDQDDGGYNAGHDGENTGIGEENDLAASLSLGAHAFEPGEIDQDHYDDEFRIEDDRFTRAKPVWNDVKFAFFFGAVFLAFVVTSMVMIIKHIEEFVSESAMPSTLPNSAFFEFKTILLILFISMISFGVSLLIFLLAGKNSAKFTSVGLKVVVFFLAISGVAGFFVGQHVQAVVFVALAVASVVILVKYSPLVSLASTILNVVITVMKRYPSTAVAALIGFLSSAFFISILTLAIGCTYIAFGFHSDGSVRFDDDGNPTSVVTAKLVITILFLNFSGLYIADVLRNVMHVTIGGIYGTWYYLESTFEGMPTGEGTGSLKRALTYSFGSVCFGSLFVVLFQCVAIFFMINSNSLFGYVGHLSMNLLGMGVGYFNLYAYSFVALYGVSMVRSARSTYLFFRQRGLQAFLNDFVISASLGFYCVLASIFGTIITYIYLVVFRRLLGLTDDAFFPLVSYSFILTFNITSVLITTIVSGSSVFFFALNKDPAVFQESHPFEFQEISRCYPKVLSKLQLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLU5</accession>
    <name>A0A1E3NLU5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_10141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018223.1">
        <property type="nucleotide sequence ID" value="XM_019159635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLU5"/>
    <dbReference type="GeneID" id="30176322"/>
    <dbReference type="OrthoDB" id="4013061at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="482" mass="52479" checksum="39D220A89376926F" modified="2017-01-18" version="1">MSIRSVPQSPTQNVLLSSPRIQMEHHHAHRRLSLSPQLPISQRSNGGSFSSESTKSSKSPKTTRIITHLSLDMSRQPQDIQQVLAGNVEDSPQLLATSVGAEVGSELVPSISNISLLSLNQQQNTLTSQMLTDAYSADTVISSATANSQDMSSRFNTSSHRHLTLQRVPNSLPLQHTQFSIGSRKSSFNSPTLQSPQMFIPQTRSSLRRSSSNSFQQPPPQSVSGQYRSQQPKQPIQIKQRAINIDDSYAEPDSPALVPISLAGSPSNFLLSHSSPPNSYKAASLLPSISQPLPRNIGNSNGAANNYPLPSTILPQTATNRLFSSRPQSSQLKLSLNRFSDDYLPHPSLPLTRADSLTDSIGGRSPELAPVSSTLPPMTPLILSMPSELSSRSNSNVVVDDDCDDFNSGDSHHHSRSDGIKDGNSFSSSSDKHIRTMEGTNHSTTMNDYNNSNISRVSTHTQKDEQDSFSQNDDIFGETIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLX4</accession>
    <name>A0A1E3NLX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018255.1">
        <property type="nucleotide sequence ID" value="XM_019160681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLX4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLX4"/>
    <dbReference type="GeneID" id="30177368"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815:SF1">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="786"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="87716" checksum="B09A7D8B0A92EF7E" modified="2017-01-18" version="1">MNKSIADDYFSMTHGPRDGALSPLTSGTPGSNPLHLTLSWGNTPENEGLSRVKSHTGAVTYKSTVVSKSQSDNKIYPYHTVHLTVPVPSKLRPLSPEDVLRRVTGKCMTCSGVSTSICTHSPAEEDGSTDAAVVAAVVGESAGAPAIPVASASRSRRVIKPKSILSKKEAGSTSIESRFANSVSFDTVNLKFSDDLNNYPLSAFSDSDDDEYDGGNLSPVKQSGVSADDLNRGRSKMEVPSRRTSSRSPSRSLSPRARSPMSSQLDMISQKEESMKGFFSLKYPTSPIITHDACTLTRTHKLFDDMYAGRLKYVTPKLHNRVIMCYISGRRHTWVSVDWAANQLLEDGDTLVVVASIKNPSRSLSRFQRRNSDAFVTSNITENRIRNSPEYAQAATENVMKYILSIINPERIVKVTVELAIGSTGDVFKDMFELYQPSLVIVGAKPGRAAPTKSWATKRISDRIVKTSPVPTIIVSPINMGLYETKFFKLLDKRMAFIDGNNQNTYRENHDLLNEIDNAGVYNLEDQREYIRKTASNDDYILKELNAIVKEKEASGNQDVDLSDDGDAESVSSSSDSGSVGDVESRLDDSDRALDDSSVDSTNPPAFVVNTNNTASFKLKRLELETQVVIYKEMAKLESEPLAKDSFKHLLTVVSDAAYKYGVQLAESAKLGGEESQLVRTLTGAPEQLERRKSMVTEALDHDDFDEKLKRFRQQKKLEKQQMMAGGKKKPSIKAPKINIETPGAAKGGFGLRPPKSPISIVSNGSSSSSIGSTIDSPLNTAKEEKKKKKKSLFGLFGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMA5</accession>
    <name>A0A1E3NMA5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15247</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018380.1">
        <property type="nucleotide sequence ID" value="XM_019160735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMA5"/>
    <dbReference type="STRING" id="763406.A0A1E3NMA5"/>
    <dbReference type="GeneID" id="30177422"/>
    <dbReference type="OrthoDB" id="67716at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056548">
        <property type="entry name" value="HEAT_Nup120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021717">
        <property type="entry name" value="Nucleoporin_Nup160"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23300">
        <property type="entry name" value="HEAT_Nup120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="5">
        <location>
            <begin position="85"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin nup120-like HEAT repeat" evidence="6">
        <location>
            <begin position="774"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1095"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1104"/>
            <end position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1116" mass="127134" checksum="F64815DA187AE2A2" modified="2017-01-18" version="1">MAGETSYLYTEVPVTLEKDFVKVPLPQDIHKIIKKLPLSETDVPKYRASTASNHETHFARFYRPESDDPLIPLYSYRILKSSNGETGDVLTICPIQINSFDKLPLQSFSFMLPSSIVQNCITFEENDDHIVIDIILSNNVVVTLNLDTRIFFNDNVISLDNFYSWCTYSMPYSFDQRKALFMKSFDVLTSIISTVDGGLLLMRRLNISSEYTVSPLTNTSYIDNIRSKLFFGSSNNVRAIDFNDQSVSVNSFIDVVKINSDLFITISVSKTFTIWSMKSQKILKEVSLNDFLPDSMHSAVLSPSCPNSILQLSNDILTVLLSLDNTYIYTFKLNIDDASLELIAELTSPNCNQNWIPIDYCLMSRNGFLKIWISWIFGDLCLYQSCKLYNDSKNTVEWSSAIEADVFGELQNSEFINAVNNMKDNKMLNVYSLRFIKSQYSDKTIQLALEIFNSHYESKFLSKSIDDQITELIMGNGGATENQKTEWIGFASVCQDIHIRTASKVFAISFDNSGVDFSDDPLVVVLKGNNSFSVLKRSTTFELLYSNSVHTRKLNDKDFPYADEINVTDLMGLVSLILEYSKGFSVEVSSAISEFIEDDFGSKKENFSELMTAIFERYIIKVANETVVSELLNRLSSINSAGDLINFLSILLTTNHIDYRSGGLGSFTEFNEGLINKSIIFNNLFAKQIIFGLMLILLTMDMSKPIEQLFRKLHEAFKYIKFIEGVSRLPKKDLLIKYLMNVNSGIFIKSSTLNLLLVNSLEQLCDEKFVFFVISELLISKNSQIAAEFVNYLPPSLPVSTILKGLIALDRRDAEEAKRIFMCNIKEITSYSKKITDGEKSSLESIRSTISLILVDSEMDYFFNLALLFESKKFYLQSLSFALESSKSIIKTKNIGTEKESDVFYKIFELALNLGEYKMSFSAIKEMTHKNRDIPLRKFVYKLFQEGKLGYVIEFNYGEDLDRVDDLIYSMGEESLQSIETLGDVKLALKYYRVCYSLRLKEGDFRGAIESLYRFNKVVGLHYGDAVYTSEEMAEILKNNYLVMLNLINSFKEEDRWIIGRKVSDGDADAVVSSAKLEEEYLTFVDKGKYNSRGKGQIQGKNQGKIDAEERGRYLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NME7</accession>
    <name>A0A1E3NME7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanine nucleotide-exchange factor SEC12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_27781</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the WD repeat SEC12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018384.1">
        <property type="nucleotide sequence ID" value="XM_019162514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NME7"/>
    <dbReference type="STRING" id="763406.A0A1E3NME7"/>
    <dbReference type="GeneID" id="30179201"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="11">
        <location>
            <begin position="266"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="395" mass="43652" checksum="99660396427A936F" modified="2017-01-18" version="1" fragment="single">VKLKSYSLNLGYPAYGAQFVNDNTVIVAGGGGDGNNGIPNKMTAILIQPQNLKKPIKRYRELTLNENEDSVMSLGVGNGTILAGINENPLMMAKGVNKHLRKFKFENDHLKFVESCQIHPNTNSTIYQKITAISNDGSIGVIVMSDTPSSIYIIETAHDLEEKFKIVTDGDVKDVTISLDGKLMCYITSSVFEVISLVTGRSVYKTNIDFLMSKIRFLDNNEVIISGAKNKDTVIAKFSIASSKIIQQKIVFKNLKGITSMDVNIKNNLTVLATSNYSLLIVRTSDLKVIKVLNKVHDFAITKVVFSKNGSYLASCSAANTVNIVEIPENFAASKSILATVFQYIFSIIMIVFLSMGVQFLYENGYINVAYTKALEMYEHYKPQDSSGYFTVESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMV8</accession>
    <name>A0A1E3NMV8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_22395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017846.1">
        <property type="nucleotide sequence ID" value="XM_019162256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NMV8"/>
    <dbReference type="GeneID" id="30178943"/>
    <dbReference type="OrthoDB" id="295029at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF14">
        <property type="entry name" value="SIT4-ASSOCIATING PROTEIN SAP155-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="851"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="878"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="891" mass="102691" checksum="31571734C091708D" modified="2017-01-18" version="1" fragment="single">MSAIWPFFNHSFSNANINKILNEIEQDASRRTSQATITSVLTATSKSSGAKSAATQGLSSKYHQLTGNSSPQIEPLQFPKSNINVYLFNKLLDQPNLIDEINLSKNQKLINYLSQKDVINTMFEYILFSMKLSKYSNASELPTEYLTHDIFTNENENENGNENENEDADKLQSALQRATVISEIMILPNSNIHQTLLTNFDIFTTLWRGFMNNDPRFYFKNIKLIETPDDDKPAYNITGLETSPFENSHEIIMFNNFLKFVDDLALINIGGFMNFIRFEQEYNCLTDQFVKFIPYSQTVCDLLVRLISTDKPYNSNGLITILIDQHLILNLLKICKKYYLDHQVQDNVCNLLNGIVGISSNIGFWEDHIPVNNNGGNNQDEQPDANNNNPNIGPNDLTRQLVSQECTEEMLDIILTHGNYGLVTIVSVIIEVIRKNNSDYDEFDWISSVNFNDENKSMPSSRDPIYLGNLLKLFSLNLDKIVQTYLTDGYYDFKKLDCLNSSIGHKIEPLGYERFKIMELIAELLHCSNMILMNKSIELDKLIYKRDSLRDIKKTEQLVSDAINGLIINNDVRGEDDEANDNDRLSLKSTSTIPYIEIPLELSVGNFFKYQLLTTNAIPLITLKLVKFPWNNFMHNVIFDLIQQIFNGRLANWDEDRAVNQEETIFDDNLSLNKILIWSLFGNYSDFFNGNDNDDSVDISAGPTFYKDYECKDKDYPGYFNLSLFIVYSYKLSNKLYKASNFRFGYMGHLTLIAEEIHKFQSYVENFGLTKNENAFGLHKEAEDDIRPFYLKSSYFIFNELYESIFEHKGDFQPWLDFVNSELREISSMYNKVLGNPNEIEAEMTNQEDSHNFEKHDNQLEESSTARNAIILDNGDSDEFRENIGDDEKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN96</accession>
    <name>A0A1E3NN96_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_15512</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018690.1">
        <property type="nucleotide sequence ID" value="XM_019160856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN96"/>
    <dbReference type="STRING" id="763406.A0A1E3NN96"/>
    <dbReference type="GeneID" id="30177543"/>
    <dbReference type="OrthoDB" id="1882297at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="200" mass="22465" checksum="05626F2FDE706BA4" modified="2017-01-18" version="1">MSEFQPVVVVDGKGHLLGRLASIVAKELLDGQKIVVVRCESMNVSGEFFRNKLKYHDYLRKSTNFNHRRGQFHFRAPSRIFYKAVRGMIPHKTARGKAALERLKVFEGVPPPYDKKKKIVVPQALRVLRLKPGRKYTTLGRLSSSVGWKYESIVDSLEEKRKAKAAEYYAKKLSAAKKLTAAKATVAGSEASEKLAALGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNF8</accession>
    <name>A0A1E3NNF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018758.1">
        <property type="nucleotide sequence ID" value="XM_019163983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNF8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNF8"/>
    <dbReference type="GeneID" id="30180670"/>
    <dbReference type="OrthoDB" id="368909at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00600">
        <property type="entry name" value="Sm_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="46"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="125" mass="13689" checksum="598D4E47D5CA7867" modified="2017-01-18" version="1">MTKKSFFVTSRATHVQGSCPLAGTPFARETATSRRPRAARPADMTAAPLDLISHSYTFDITDGRVIQGVLIAIDDQSNLLVTNAVETCGSHHRELGLVSIRRNTIARVLTTEREYSSIFARNSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNX8</accession>
    <name>A0A1E3NNX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_32500</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018902.1">
        <property type="nucleotide sequence ID" value="XM_019162769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNX8"/>
    <dbReference type="GeneID" id="30179456"/>
    <dbReference type="OrthoDB" id="10256233at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990400">
        <property type="term" value="F:mitochondrial ribosomal large subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902775">
        <property type="term" value="P:mitochondrial large ribosomal subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="154"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="370"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="525" mass="58894" checksum="C9D7CE39A07C0875" modified="2017-01-18" version="1">MFAELHSRALHRQTAPSLLRLAHFSSRGKSAPSSSTRKDQGVARRGGSKNFKLPTVDNYPPYKFGSYSPLNLPDERSREGIKKLVDKITDFNELKIYPEIRSALIEEIKAHTVLRSQNYVSNTKKVKTEGELNGLIIRPTPIQTAAIKIINDKRQNHEFLKVFTLAAETGSGKTWAYMAPLLQQLLEVVEATSPYIKHRAGIKSVILVPTHELVDQVHETVEYTAKKLNLNVLKWDTESNFRVFIENFKKGIDIMVTTPGKLNSLSRYPSVQNPKMIFGSVSFCVADEADTLMDPSFVDETNNIIKHMTNLSSLVFASATIPSRFNKTIDHLFPDVINISTPSLHKLPKSIEFRMINASVSPYKGSKIKALAQALYAIHCDGTEQGYEKRVIVFVNKKEDCSKIAEKLSKSYGHDVTYITSEDTAEERKRKVATFVVPAQKLEDPAKPVLKVLVCTDLLSRGLNFQGIRNVILLDVPKNSADLVHRAGRTGRMNQSGRVFMIINDSDKGHIKGLPKVLRNNRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNZ6</accession>
    <name>A0A1E3NNZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_119403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018922.1">
        <property type="nucleotide sequence ID" value="XM_019159860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNZ6"/>
    <dbReference type="GeneID" id="30176547"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="159" mass="17296" checksum="227D1D7D26772971" modified="2017-01-18" version="1">MNPVLRPALPRFSLRRAVRHGHKRADAGNVLAGFCGHLAGDWMRRRSSAPPPDRPWGKLTSCVSSGCPSPPLRRGTERACTGPDGSVWSVHCLHRLHWFRAAAPSFLCSSGKRHRQAVPGHEPRAASGFMPEPGLFPNPAAAPKKKAAGRSTQKTRTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP12</accession>
    <name>A0A1E3NP12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15368</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018531.1">
        <property type="nucleotide sequence ID" value="XM_019160795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP12"/>
    <dbReference type="STRING" id="763406.A0A1E3NP12"/>
    <dbReference type="GeneID" id="30177482"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="514"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="867" mass="97693" checksum="9488B1E296C2AE43" modified="2017-01-18" version="1">MMNLEQDKEESLMEKKITRSRNGCFTCKKRRRKCDETKPYCKNCTESGRVCAGYGVRLVFDVDDSRNSNDSFNIRGEKKHGFRGRPKLKDAGDTFIINSHNPHNPSAEEISVKMEENGVEDTTFENHKSRTETRRKLESPSYNEDRVRKSKHPRVTKHAKPQIKSLISSNEYSKSGSLPNVDSLLNPSNIDLTPSDSPSLLVSRDSSSNTILSNTNSNINILSAPNTGKNTNSVSTTSDRDFELDFNDPQQFERAFFSGLDYILDNYDNGLLNSVDFSFLPLDSSHSNTSMNNHVNMNMTTSSSGLNLPPSPNYSNIASISPAIPSASLSNVQSYNGVGNVDSHNLPYHNNSHNELELDSSHLGPQLKKREEILILKHFFEKVIYLLDAHPQTPWPQLMMKFGSMDLAKSCFLSLSSMHLYVNNGGDEFYKKGLLHINNTMEYLIKYVKTGGIANTNTNTNTSTTVTRDKKGHSDANSSTSGSASNSDENETEHNVDCNDQNNSNGNSNDDDHDERNSEEQMKDSNDLDLNVPKIISRIKSESNKKKGTNFMVILLLLYVHLLFAVLESGRSALARMFLKLSASIASDPLFNKKMKKIQQSQSLLCVLSWFDTVSAIVSPDCRIPYCDPKWFGESSDIISTDKMNGCPVGMFRVLYDLCLYRKKVMTLFSQQECLLQQIKLSHKNLMDLRDRCLHYRDHVLYILPHGSAFSYIDRLKCAQLWSLAAILVTIQLELHYFSKMSKMIEHNRNMISASEFPDAVPSVHIHSEKATAIVTEFLSVYNTIPSSSPIITQMVWPIFYVALCSSTDENRKQSWKALEVLYETVKMGTIKSNMDIVMRVWEEGCSLESILGGEGWFEAGIDLLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP38</accession>
    <name>A0A1E3NP38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial genome maintenance protein MGM101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_26897</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGM101 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018561.1">
        <property type="nucleotide sequence ID" value="XM_019162469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP38"/>
    <dbReference type="STRING" id="763406.A0A1E3NP38"/>
    <dbReference type="GeneID" id="30179156"/>
    <dbReference type="OrthoDB" id="17164at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000262">
        <property type="term" value="C:mitochondrial chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009446">
        <property type="entry name" value="Mgm101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31404">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE PROTEIN MGM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31404:SF0">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE PROTEIN MGM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06420">
        <property type="entry name" value="Mgm101p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="178" mass="20560" checksum="8DDAF098CB378271" modified="2017-01-18" version="1" fragment="single">DSTKNWSESFHGLGNEPFSKESQETLYTELPDDDIEITPDGLLYLPEIKYRRILNRAFGAGAWGLAPRSETLIQMQEFGGLLTREYGLVIHGRLVSIARGEQTFFKEQGIPTATEGCKSNALMRCCKDLGIASELWDPRFIKKFKKKNCENIFVEYVPTKKKKKIWKRKDEEVEYPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPB0</accession>
    <name>A0A1E3NPB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sedoheptulose 1,7-bisphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019009.1">
        <property type="nucleotide sequence ID" value="XM_019160957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPB0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPB0"/>
    <dbReference type="GeneID" id="30177644"/>
    <dbReference type="OrthoDB" id="4818801at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050278">
        <property type="term" value="F:sedoheptulose-bisphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046390">
        <property type="term" value="P:ribose phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000022">
        <property type="entry name" value="Phosphoglycerate mutase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF15">
        <property type="entry name" value="SEDOHEPTULOSE 1,7-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27702" checksum="A57F52C147617412" modified="2017-01-18" version="1">MPNESHTPRCILIRHGQTEWSKSGQYTSVTDLSLTPFGVKQMEITGEHLIGDSHKYLINPEVVRLIITSPRKRAIQTKELLFKHVDKKVYEAIPSEIDEDIREWEYGDYEGLLTKQIKELRAKRGLPYEDWTIWQYGCENGEDYKQVTARIDVLIARIQAIHKEYLDKKESCDIMIVAHGHILRCFAARWIGLDIHRNPRFMLDAGGVCVLSYQHHNIKEPALYLTGAFTVPLAEEGQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPW2</accession>
    <name>A0A1E3NPW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PICMEDRAFT_15962</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019252.1">
        <property type="nucleotide sequence ID" value="XM_019161037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPW2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPW2"/>
    <dbReference type="GeneID" id="30177724"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903459">
        <property type="term" value="P:mitotic DNA replication lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 20" id="KW-0408">Iron</keyword>
    <keyword evidence="16 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="22">
        <location>
            <begin position="237"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="21">
        <location>
            <begin position="533"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="23">
        <location>
            <begin position="1002"/>
            <end position="1072"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ODQ48139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1094" mass="124838" checksum="A6399569527068AF" modified="2017-01-18" version="1">MTTVKREAGHELDIFELENKRRRLTSDQVSKEGFVIPLNNEEVSTESIHKKLNVSETEAEPSTFEKELITMTQAQPVEAKARATTQVWTRPPLPADFTPMTHEINFQQLDAEESSIVGPDGRDQGCVRFFGVTDKGNSVLCNVLGFRHYFYVPAPRGFPTEFDEFTRSLKSQYQNMIEDVGIELKESIWEFHGDQKVPFLKIILSHPRHAGKVRTGFERGEVQFRDLFNPTGTVTFDNLQYLLRMMVDCQITGMSWLSCPAGKYTTVEYRRKVSTCQLEVNINYTDLLSHKPEGKYLSMAPLRVLSFDIECAGRKGIFPEPDQDSVIQIANVVSKVGDSIPFVRNVFTVKSCSPIVGSEVFSSEEEGDMLMKWKDFVVEVDPDVIIGYNIVNFDLPYLINRAKALKLDKFPYFGRLSNTRQEIKDSIFSSRAYGTRESKIINTEGRMQLDLLQFIQREFKLRSYTLNAVSSHFLGEQKEDVQHSIITDLQNGDSETRRRLAVYCLKDAFLPLRLMEKLMCLVNYTEMARVTGVPFSFLLTRGQQIKVISQLFRKCLDLNIVIPNMKSENVNEEYEGATVIEPVRGYYDVPIATLDFASLYPSIMMAHNLCYTTLLSRKTIERLGLKEGEDYVKTPNGDCFVMEHVRKGILPTILKELLGAREQAKKDLKNETDPFKKQVLNGRQLALKISANSVYGFTGATIGKLPCLAISSSVTAFGREMIEATKNAVQEKYSLKNGFPYNAQVIYGDTDSVMVKFGFSDLETCMSYGEEAAAYVSKLFKDPIKLEFEKVYFPYLLINKKRYAGLFWTNPAKFDKMDTKGIETVRRDNCRLVQNVITRVLELILEDRNVEEAEEFVKKTIANLLQNRVDLSQLVISKQLSRQDYANKQPHVELAERMRKRDAGSAPNVGDRVAYVIIKTAAGDKNYEKSEDPLYVLENSLPIDTKYYLDNQLSKPLTRIFEPILGEKRTRELLHGSHTRIVKVGSAKTGMLMRFAKKSNTCKGCKTAIPDNETLCKNCKSRSGQIYGEALGKMNLLEERFSKLWTECQRCQGSLHQEVLCENKDCPIFYMRMKCQKDISKQSAELQKWDSSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX5</accession>
    <name>A0A1E3NPX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Agmatinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019199.1">
        <property type="nucleotide sequence ID" value="XM_019164089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPX5"/>
    <dbReference type="GeneID" id="30180776"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000014">
        <property type="entry name" value="Arginase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133470" description="Agmatinase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="42718" checksum="344A2A368268727F" modified="2017-01-18" version="1">MISVLLLSLAATVPVKAVLGEGYVNGQLQLAFGGYASEPTLEDIWGEEWPFNGIPTFAHLNHTKCLLSPETDFDIGIIGVPFDTATTYRSGARFGPRAIRSASQRQAQLRGFNTRAGINPYNNWASFLDCGDIPISPMDNTIALKQMTMGFEELLLRRFSSSDKNHPPRLVALGGDHSITLPHLRALKQIYGKIAVIHFDAHLDTWSPKGYPSYWSSNQSEFTHGSMLWMARNEDLISNDSNVHIGIRTRISGTTWEDFDDDSRQGWKRYSADDVWLEGRQGLDKIVAETKARIGDLPTFISLDVDCMDPGFTPGTGTIEPGGMLPREVIYLLRKLDLNLVGADVVEVAPSFDHAEVTSTNAAQVVFELITTMVAKGKPLPVVRSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX6</accession>
    <name>A0A1E3NPX6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_11037</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019228.1">
        <property type="nucleotide sequence ID" value="XM_019159742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPX6"/>
    <dbReference type="GeneID" id="30176429"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="199" mass="23020" checksum="74DABEBDD44F2D30" modified="2017-01-18" version="1">MDEILADEKVAIHICPRIKKFTEDGVEITDGQKGENFDKILFATEYHMWYPFLNIPENEGMTSRTTLTQNYAHTEVVNVYLYTFSVGEPTLSHIGIPQNPLFFLTAEVNAIALAGLWYGYKHLPPTAEQIEWCSRRLRGKNRSFELFDETSIRPYYSKLYELAPADKVDLQQILRSDEIAKAKNVLKKLFYAFASRELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ23</accession>
    <name>A0A1E3NQ23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial intermediate peptidase</fullName>
            <ecNumber evidence="4">3.4.24.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_32396</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.</text>
            <dbReference type="EC" id="3.4.24.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.59" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019278.1">
        <property type="nucleotide sequence ID" value="XM_019162763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ23"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ23"/>
    <dbReference type="GeneID" id="30179450"/>
    <dbReference type="OrthoDB" id="17530at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="14">
        <location>
            <begin position="299"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="790" mass="88980" checksum="B98558BD69342B9F" modified="2017-01-18" version="1">MKLVIRSLRAAIRSPFVNSRRSLTTSTSHIQTSNDANEDEIVKIFDSSDYWNKFNQQQSSDKTASNQTASIFDSLNFNSSRQKSVGLFCNPYLTSPKGLKRFAEESLDRARALTNHMIEDQSEKSMKSYIKELDRLSDMLCRVIDLCEFVRAAHPNSSFVKAAEQCHAQLFEHMNVLNTSKPLFVKLQKVLYDGKLGIRKTLSQEEISVGELLYSDFKKSGIDMDDATGEQFVQLSSYIATAGQEFNNNITIPQSNSIKIEPSEWEEGDINPECSKSISHGFDGSLTIPIYGHLPFELLRSCPNKIIRQKIWVALHSVPENQTALLTSLLSCRAILAKLMGCNNYAEYALQDKMARNSNNVVGFLRGLINDLQPEVLKELKMLYKYFPPGSVKENPTSEELVNSIRPWDREYLLTKHILSQKSSQNEDITQYFSLGTVVSGLSKLFHSIYGIKLIPQKLKPGEVWSSSVRRFDAVSDKDGKVGIMYLDLLHRVGKTPHPAHFTVCCSRMLDNAELDSKDPFNLSLPTTSTVETVVQKGKTYQLPVISLVCNYYGNKNNSGIICLTLEQVATLFHEMGHAMHSMLGRTALHNVSGTRCQTDFVELPSILHETFARDERVLASFGKHYETGKPVPTQLVRKHLESDRLLTYCETFGQVKMALLDQLYHGAGNCDVTDEAFDALGLYHQLESNLAILADTKSSWPGKFGHLYSYGALYYSYLLDRAISAKVWSNLFQEDPFSREAGEKFKNEILRWGGSRDPWVCLSKLLNAKELAKGDVKAMEFISKDSGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ32</accession>
    <name>A0A1E3NQ32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15991</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019288.1">
        <property type="nucleotide sequence ID" value="XM_019161044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ32"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ32"/>
    <dbReference type="GeneID" id="30177731"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="458"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="348"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="541" mass="59076" checksum="A1EBF67AFA575BA5" modified="2017-01-18" version="1">MAPLFDTQFQSTSQSTQGQDQFDWVGPASVGANANTKANAAAPALEKPALNLSNSLSSIFFNNIYNDAETAGVAQQDIPETRQDDAGADISSTLLQLINEKPLVTDMKSTPRCATRLNFDVNPLLFTQPVTGIPPADVSPQFKDPQSISQSGVNNDNNSITNNSNNTDEQYNSLFTYLMNANQDVSTPANDYYSIHTPVASDMSLSPYQRRRLESGVSGSSANGGSDDFLMMQGDLLSDATSVTYLDEHSLSKFNDFSNMGMNPNTNTLGGVDTGSDRFNTDNNTMARDRLFSSFSEVETLNEEMKEALNEARLQLPISLPSQGMSLYSEKGDGQGVAEGSSRRRSDVTSAHTGGLNNYQDMDSNDESNEFGTGDSESDGENNGDDDDNGDNNDSDDDTNISGNCSDDDSFEGTGEVKRGAMAVKRARNHARGKSAGSGSTAFAGGLANGIGKTDKQFKCSKCTSSFTRKTRLTEHVNRVHLGKIYHFKCNQCGTRLSSKENLTRHSIVHTDKFKCKRCNRRFDRSYRFQRHLEKCNMAHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQB4</accession>
    <name>A0A1E3NQB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16046</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019349.1">
        <property type="nucleotide sequence ID" value="XM_019161070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQB4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQB4"/>
    <dbReference type="GeneID" id="30177757"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002130">
        <property type="term" value="P:wobble position ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="961"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1023" mass="115507" checksum="91024E2DEEDCD844" modified="2017-01-18" version="1">MHPVQHMGPVCSLKVFSNKWLFSGEGPSLKIYDYVTGEKLFERIIFKRNKIHGIEYIDSNVDGKVESDVKIVVWGGRSLSILTLAQLKNPNFEIPHQGVGDWIFHCIFKDQHTLHILNSHNIVHTISTENEKPQLLSSKSCDWKSILYSGTLHKNTETGKVTVLAGTVMNGILIWDLKSGEVQNNLTQHEGSVYCVVSSPDGKYVVSCSDDRSIKVWNKKTGKLLANGWGHGSRIWGLSVYNVTDSGFNIFSSSEDCTSRIWTFNYSGSDELTQDKIILGHTGRHVWSVAVDDQNKIGFTGGADGKILVTDLATDKREGFWGHKWELKDVSCETGYNFIKGELIKEYVDFGHGLLAVTSGGKLMILKHYKKWLPLFEDSKFKRSCLLKAFKEDPIAVIGNKHGDVIIMKFNENCELVLKNELKLEQHFSRLGNILINQHNNKLFVLFESPNPKDCIVYSEIDRESLTVMSDKLLRKPIDKIILSSVAYDTQRDYLFIGCRFATLLVYSMSDNQNEPIAIFKNFFKGDTISSIEPLNESQECTLYLTDRDGSYHLIKINEELEYEFLQSSRIQKGFLEGIIRLSNGDTIFYGFKSDSFFAWNETKQYEIMREICGGPHRRWMFKHWIDKTSKNLKYRFVYTRASEVQIIEIGDSYAVELLAAGLHGREIRDICWIDAVNDKNEKVVITGAEDTTLKIGTLKSNGEYTLHWTYREHVAGLQSIHPVNSEYIMSSSALEELFLWKITKADGKKCMALHGSLPPSEENPDLRIMNFDTIEIVDSEKNLVGFLLVSAYSNSAIKVFQYTYSTKKFTLLIDDYYMKCCIFHAKFLSLNGKLYILIGSTNGHITLYNVQTNIEDRYITLDVEVCGKVRLQEKTPTADPKPSKLGKWTINQQMHQSSIKALELIQVSPDKVTVLTGGDDNALIVSKLQSVEGTEVVTVDVKSFNPSAASSTITTIKAVDQNTVLVGAVDQSLRLWDIRSDLKLVEDNYTTVADTGCAEIVDFGDDKKLALVGGSGFSIIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQN3</accession>
    <name>A0A1E3NQN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_47464</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019533.1">
        <property type="nucleotide sequence ID" value="XM_019163130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQN3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQN3"/>
    <dbReference type="GeneID" id="30179817"/>
    <dbReference type="OrthoDB" id="421009at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090083">
        <property type="term" value="P:regulation of inclusion body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021538">
        <property type="entry name" value="Syntaxin-5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF3">
        <property type="entry name" value="SYNTAXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11416">
        <property type="entry name" value="Syntaxin-5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="246"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="337" mass="37289" checksum="09DCAD13BC3F154C" modified="2017-01-18" version="1">MEVYTANSIQDRTFEFQQAVNTFSRQEKAHHKEASSGKGNSGGKTEFNKQAGQIAKEIIRVTGALSKLAQLTKRKQLFGDKPTDIVELTYIIKQDIFRIEKELKSLKSLQVSGHRGKSNSGSDAQLNMYNKNVVQLLNTKTKNISENFKDVLQTRQRTEMLQRSRQEQLLAAVKSIRAAAKNMAGSVGSPGVGTSENPFLASLDLGTAAASGPGAASDANHDDYGGAMLSLPDQSQQLLLLEEQNNEYIQERSNAVDAIESTINEVGGLFQQLATMVQEQGEVIQRIDTNVEDVSLNIGGAQRELMKYYHNVSSNRWLMLKIFGVLIVFFLMWVLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQV5</accession>
    <name>A0A1E3NQV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019587.1">
        <property type="nucleotide sequence ID" value="XM_019163553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQV5"/>
    <dbReference type="STRING" id="763406.A0A1E3NQV5"/>
    <dbReference type="GeneID" id="30180240"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="14"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="135" mass="15030" checksum="0322745789D99E8C" modified="2017-01-18" version="1">MIASRQGIFAKRGLAFQPQARLFHASRCAQKVTEVSTMEAFRETAMKSPIAFVDFYATWCGPCKMISPYVEKFSEMHKTVDFYKVDVDEATEVARDFGISAMPTFLLFKHGKVVEKIVGANPQGINSALIKATQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR80</accession>
    <name>A0A1E3NR80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019705.1">
        <property type="nucleotide sequence ID" value="XM_019160240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR80"/>
    <dbReference type="GeneID" id="30176927"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="128" mass="15563" checksum="DF64925DBA0A1D49" modified="2017-01-18" version="1">METQWWNTGEVKQFIVKEKYEPPFFTEKRGIKDRRINPRRLVDPILRKEENSRSLIKDVISEIVSLHPDTYRDIPEYKRSRSFYVTSTQDRFAVFKDEKDTLQLIEDFADSTFRVVKNSERWMKDILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRB9</accession>
    <name>A0A1E3NRB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ER membrane protein complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_16039</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019341.1">
        <property type="nucleotide sequence ID" value="XM_019161065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRB9"/>
    <dbReference type="GeneID" id="30177752"/>
    <dbReference type="OrthoDB" id="1894652at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21203">
        <property type="entry name" value="ECM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133471" description="ER membrane protein complex subunit 10" evidence="3">
        <location>
            <begin position="22"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26858" checksum="6AFAE698DD151A44" modified="2017-01-18" version="1">MIQLFCIFAIVLLHLINGANCTQSYDLLIHPVGVSSANGYPLSNLSINSSTIDSQLLLNKALATKDLTESPNGWCLSLRSQPSDELTASGLECFNYFKPVQSLSTHNIAGELGLVLDVEHKIVSVNFYPKEKGGLSLHISKSYEISDPQPRLRLDGKTDATDDVDIPKKSTKKQKNLEPKGKILEENKEDETSLETDELKDFQEIVKPRGLYSDEKTFIEKYWVYIVPPMLVIFVVGNLGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRD1</accession>
    <name>A0A1E3NRD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14182</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019755.1">
        <property type="nucleotide sequence ID" value="XM_019160262.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRD1"/>
    <dbReference type="GeneID" id="30176949"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01380">
        <property type="entry name" value="MYSc_Myo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036103">
        <property type="entry name" value="MYSc_Myo5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="71"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="10">
        <location>
            <begin position="1249"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="8">
        <location>
            <begin position="640"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="165"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1498" mass="172745" checksum="5C94A0C25677BD42" modified="2017-01-18" version="1">MRLEQGTRCWIPDNRIGWLGVNVTKIEYNENSKVYKIELANDEDSNECFVVETNNLAENNEKLPKLRNLDDTVDDLTTLSHLNEPSVLNSVKLRYSKKIIYTFSGIVLIAINPFDKLENLYTQEIIQKYQNAKKNDNPPHLFGISDEAYKCMRKDSISQSIIVSGESGAGKTVSAKYIMRFFASVHTEGTQINTIEIEKQILATNPIMESFGNAKTTRNDNSSRFGKYLRISFDEKSVICGATIQTYLLERSRLVHQARNERNYHIFYQMIQGLPENLKKELSLQHCGNYHYLNRGNTSAISGIDDSKEFRDTCDALKIVGIDENKQFELFKILAGLLHIGNIIIQNSRGEAVLASDEPNLLMACNLLGLDPKEFSKWIVKKKISTRSDNIASNLKYHEAIVARDSISKYIYSLLFDWLVSYINSHLCSSENEAREKSFIGVLDIYGFEHFDNNSFEQFCINYANEKLQQEFTHHVFKLQQEDYISEGIEWSFIKYTDNQSCIDLIEDRNGILSLLDEQCKLPAGSDKAWAEKMFHSLTKPPQNKVFKKARFGTEKFIVSHYALDVTYQVDGFLEKNRDTVSDAQNEVLRLSGNHFLKNVILSIEKNRESPEKSPVTKKRLSLGGRNKKPTLGYLFRNSLNGLMATINSTNAHYIRCIKPNETKRPWEFDNLMVLSQLRACGVLETIQISLVGFPSKYTYEEFLHRFVVLLPAEVETDFKRGEIQSEVEMKKLSTKMLRKLISDQSSFQTGKTKLFFRAGVLGQLEIAKTNKIKSSVTILQKHIRRYLERKRWLDAQASMVDLQSVVRGSLIRTRTRNQESSCIAIQSLLRGSLARKQISKIINSTFIIQSHVRGMIVRNEYRELVRLRDAARRESEKNEKEAKISNQEHQKKLIEMQERETRILQEQVKVENKQKKNDNERHMEDERPKQKAQEEFKLAQKLSQKKKLDEVDQKHANGRTVDPIKYIESDSLHKSSNMSKLTNASFADLLPITNHDEGADEIWGILENSLHPLKYSYNDLLGMRPSKSLAIITRFIVLRIKNISESIDSYEKQHPHISDVYLTERLEKNTRNDIMILKKMMRRVKYAKPDMAVKRLSVKSVNSISTEKIMFGQAKADPSHLDRNFRSGYADLLDYMKGSPIASTLVANLLLQDIRPPPEESEEPISIGEVLFPSRLVVAILTDLWRIGMINQSAVLLEKVMNIFKEKIETLKDPEALISNGAYLLNNVNEIRTCIAWSRYTAMSDFDSKIFLKAEERQDYLKMLLIMKRYCETVFGQMYNVWIKSILNELNKKTIDAVILDETLYSIRQNPNVYFRHMFHKGPKYKMFDVLRIFNNVYLAMKSYCFDEVISTSVIHDLLTYTDVLCFNDIITRESYLTVDKGLLIKYNISLLIDWCNEHTVPDSPNKLMHLLTLCQILQLKKQGFADIDMIATTCDSISRKQIEKIIGECDKNSTTESGKKLGEKTKKLKKKDQSNNSEFVIPLEDDAFENAFLCMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRQ2</accession>
    <name>A0A1E3NRQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="12">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_14275</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="12">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="12"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019864.1">
        <property type="nucleotide sequence ID" value="XM_019160306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRQ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRQ2"/>
    <dbReference type="GeneID" id="30176993"/>
    <dbReference type="OrthoDB" id="5852090at2759"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006555">
        <property type="term" value="P:methionine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="14">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="15">
        <location>
            <begin position="117"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="16">
        <location>
            <begin position="239"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ48751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="382" mass="41875" checksum="AEB2AA02B28F4CD6" modified="2017-01-18" version="1">MSETFLFTSESVGEGHPDKICDQVSDAILDACLAGDPLSKVGCETASKTGMIMVFGEITTKANVDFQKVIRDTIKHIGYDDSEKGFDYKTCNVLVAIEQQSPDIAQGLHYEKALEELGAGDQGIMFGYATDETDEKLPLTLLLAHKLNGAMADARRSGSLPWLRPDTKTQVTIEYKKEGGAVVPLRVDTIVISAQHSDDISTADIRTELTKHIIKKVIPEHLLDDKTIYHIQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVNAGLCRRCLVQFSYAIGVAEPLSIYVDTYGTSDKTSEELVAIVRNNFDLRPGCLVRELDLARPIYFKTASYGHFTNQENTWEQPKKLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV2</accession>
    <name>A0A1E3NRV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIGR01456 family HAD hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_22510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019912.1">
        <property type="nucleotide sequence ID" value="XM_019162265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV2"/>
    <dbReference type="GeneID" id="30178952"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000069">
        <property type="entry name" value="HAD-superfamily subfamily IIA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF4">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="56228" checksum="F54D83EFF7A1B419" modified="2017-01-18" version="1" fragment="single">PVPSSYLNNDNIDSMKPEHKEPVSSISLSDQLVDSFPSMGTFAKQAAADVSQREKSFSNNLSPHRFSFSSNLLSRPPQFKKEIIDHPRVASYAFAFDIDGVLVRGPETLSHGVEAIKYLDGENPYNIKVPYIFVTNGGGKHESVRAQDLSKRLQTEITCNQIVQGHTPMKELAGTYKNVLVVGGVGNTCRHVAKDYGFENVFTPHDIMRWDPSVTPYYEMSEEDLGYCECDEDVDFAKTPIDAIMVFADSRNWAADQQIILELLMSKGGYMGTISDTFEDGPPIYFAHSDFVWATNYRLVRYGMGALQVSIAALFKEHTGKELKVIRFGKPQRTTFKFAEKVLDSWRKDILEDHIEEMTSCADSTTLTSDIFSSPDDSKYKSVFPSSDSETDDRESEDEENIDGLTIQSLNKTLSCPDDLPPPSTVYFVGDTPESDIRFANSHDESWFSILVNTGVYQSETTPKYKPKKQCDNVFEAVKFAVEREHQKELEEWNKNAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS10</accession>
    <name>A0A1E3NS10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_26526</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019993.1">
        <property type="nucleotide sequence ID" value="XM_019162451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS10"/>
    <dbReference type="STRING" id="763406.A0A1E3NS10"/>
    <dbReference type="GeneID" id="30179138"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="4">
        <location>
            <begin position="139"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="662" mass="77536" checksum="27DB3921C1F9D9B7" modified="2017-01-18" version="1" fragment="single">KFILTKYTIYVTNQRMYIVGSNSRESVFRILEIDLTNPNTLTIMEDNVYFTRNEIMEVLNGIEDSSEGGLTKKLTAVGLLGFIKFTKHYYLLVVTRRKEVAVLGSHEIYHIDETELIPVTNNLKVPEKKSTEARLVQTFLNIDLNKTFYFSYSYDLTNSLQTNMIRNKRQSLRMNGKTELSMVFEFNERFMWNCALLEPVFQCFDKVYDWFQPIIHGFIDQVKMSVFQIQVYITLIARRSHRFAGARFFKRGVNDDGDVANEVETEQIVCDMLTSSFHDPTAGFYNNSRYTSFVQHRGSIPLSWSQETAPNIRMTKPPIEMNVIDPFYTKAALHFNNLFERYGTPIQVLNLIKQREKTPRETKLLDAFNNCVEYLNQFLPKEKKLDYVSWDMSRAAKSRTQDVIKWLEKYSEETLTKTGFFHNGKTISDTKLQEGVCRTNCVDCLDRTNTAQFVIGKRALGHQLHALGIIEEKYLEYDSDVTNILTEMFHDHGDTIALQYGGSHLVNTLQTYRKINQWSSHSRDIIESVKRFYSNSFVDAQRQEAINLFLGNYVFASDKPMLWELNTDYYLHNRKNISELNGSNSYTHWYNDSYLVNRKESLKSLWETTIQNKYIMELQERGLLVVKIDPYVGCFENYWNIKYGSRNFVSLNELFEFKMNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS59</accession>
    <name>A0A1E3NS59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14409</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019997.1">
        <property type="nucleotide sequence ID" value="XM_019160359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS59"/>
    <dbReference type="STRING" id="763406.A0A1E3NS59"/>
    <dbReference type="GeneID" id="30177046"/>
    <dbReference type="OrthoDB" id="6846267at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF8">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="6">
        <location>
            <begin position="15"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="541" mass="63304" checksum="A6247EB36EFC5AE6" modified="2017-01-18" version="1">MDNEIFAEKDSPKVEVTIPNQGTLTGFTLKNPANGKLTSNRFARIPYALPLKKRFTLPEPIPDDYDYTGEYRDFGFKCPQPVYESKQIVYPKSESDEHIQYLNVWVPASDKYKPKTGWPVLIYIHGGWLQYGSPNKDMFNVVELMDDENFQEKYILVTLGYRLNIFGFLTCKELLEENPKNSNMGFWDQREGIKWVYKYIKYFGGDPEKITVSGLSAGSYSTFFQLAYELYHPEETQIIKQVVFYSNMVFSQPKTIDECEDQFNEVVDKLGMNNLTGPEKLEKLRSFDFEFLEEFVPKLELHTFRAVSDSHFIAPTILGDIQSGKFSKLLIDKNLRIVHGETDNEFYLYSLFNPPTSLDTLPIEIENYYPRKVVPTLMDVYQVKDKIDPDSPELEQQLIDMFGKITGDGQVYASTRGFINNVMKGGFPSERYFRYRVGYRGKWLDKFMPPEVKVTHAYDQSIWFYILRNGYSDSEKSKMTEFVKPFLDFLNFKEDIGDWDTSDFKKFRWFKPDGSIVYEEDPDWDWGVKVAENVYRAQLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS61</accession>
    <name>A0A1E3NS61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 231</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_89753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020090.1">
        <property type="nucleotide sequence ID" value="XM_019165051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS61"/>
    <dbReference type="GeneID" id="30181738"/>
    <dbReference type="OrthoDB" id="3989852at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="44444" checksum="A167EA80B2F5C855" modified="2017-01-18" version="1">MIINFGFPLQIWKSWIYVCITIFTMFFVLLPSSVLVYLHFQSSIIPEASPPMVLQFQYMKETGPFAFYNMTEAALEQVRPWKNANTNAFTRIEQVLTVHIRYLKLTEGSTVGGVRVSVYNDKKPPILHKSSLKPSNSPDLGGHLSRNWPFKAISMVDSQNEFSLYRTDRIFKSWSEVIGKSYTKSVPFLSGVEPNNLGVESKLGVLDYFVPKWILSLFVPQGVQNLLSVKNVSSLLQRKETYEDIYDHMKHMETLALLNSYGTFQEFPSKSFFTGLILFDGELDYKEFTGTSLLVELDMNDVFVVEAYVKLDYVLSGFRWWVYWWPGLCFLIGVSIIWVVSCLGCLVFSWFTMSAWSLYLWLMETQEEKDNDTIRRNLSELLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSF7</accession>
    <name>A0A1E3NSF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_89832</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020103.1">
        <property type="nucleotide sequence ID" value="XM_019165053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSF7"/>
    <dbReference type="GeneID" id="30181740"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034618">
        <property type="term" value="F:arginine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052706">
        <property type="entry name" value="Membrane-Transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310:SF4">
        <property type="entry name" value="AFR304WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310">
        <property type="entry name" value="SULFATE TRANSPORTER YBAR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="531"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="584"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="8">
        <location>
            <begin position="654"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="872"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1006" mass="112681" checksum="DB5EE179FBE2D6D0" modified="2017-01-18" version="1">MPTSNSYGDGAFPRSIPKSINQPRQASSGNTLARSFMYSSYKPNMTSPINSISLQVHSSDSVHEDSAGLAAIAVPDIDTDDTADESPLSTTPSNTYPSYSTAVAEYSPVDETSNMLPITHQTSHASLSFYGSYDDPHVDIDPAFDLEALKTKQSSFSLRNTIDFFKEADKDTLLHECVYRPLGYMPAVFLGTLLNVLDALSYGMIMFPIGETVFSSMGPAGLSMFYVSTVISQLIYSFGGSAFKSGIGSEMIEVTPFFHQMAISIMLKLQNDERYAQSPSLQDIIITTTIFTFAISAIVTGLCFAILGKCKLGKLVGFFPRHILVGCIGGVGYFLFVTGIEVSSRLEGGIEYNWETLMYLLQPLPFLQWTIPLLLAIILIIIQRYNSNPLIVPCYFIGVFAVFHSLVLAVPSWNLGLARDYGWVFTAQNSNEPWYSFYHYYDFKLCDWWLVLDQLPTMLALTFFGVLHVPINVPALALTIGMDTFDVDRELMAHGASNFISGLCGSIQNYLVYTNSVLFIRSGADSRLSGVMLAIATSGIMFIGPVLIGYIPVCVVGSLIYLLGYELLKESVWDTVGRLRKFEYITIIIIVLTMGAWDFVYGILVGILLACVSFVIEAGSKRVISAVYTGEYARSIVARHPKQLEFLKNVGKQICILKLSGSLFFGSIGGLEDEVRNMFDVANYTKQPIKYLILDLNNVLTFDFSATEGFKRIRNLLMEKQCYFLLSSIDNNSAIVDSLKFCGLWETEEHQEKIQMFNNLNSALEWCENIFLKHYKELIAQSKQPSLTIGKEIKKKQTVGTYNGSSSGGTDSGLMFGTPRHVQFINAARRYNQKEQLQQDLIMNKLKEKDRKASVSTMKQPLYLVLQIMQGLSSIYDEKFWGRLSPYLRKSIYKAGEFIYDKNSNQPCLYFFESGLVDFKIEFNDLKFTINSSELPLTMFGDIVITKSDRTIDYKANTSCVMWVLDSSAIKKLKTENRDVYEELLVVCIRITTQRFESITSNVLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSI0</accession>
    <name>A0A1E3NSI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable quinone oxidoreductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">NADPH:quinone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_14508</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020117.1">
        <property type="nucleotide sequence ID" value="XM_019160407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSI0"/>
    <dbReference type="GeneID" id="30177094"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05286">
        <property type="entry name" value="QOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000053">
        <property type="entry name" value="Quinone oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047618">
        <property type="entry name" value="QOR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF13">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="20"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="343" mass="37825" checksum="289DE814273BFA85" modified="2017-01-18" version="1">MSSTFSIPDKQAVIYYERNGGPEVLKYSTEFAVPSDLSATDILIKNKYTGINFIEVYFRNGIYPADFPYIPGREASGEVVAVGDKVTNFKVGDKVAYLGHKNFAQFTKFDSTQSTILNLGQNATSKQLKLYAASLLQGLTALTFINEAYAVKENDYILVTAAAGGVGLILDQLIGNVKKAHVIAVASTDEKLAKAKQNGAEFLLNSSELSNDQLAEKILEITNGKGVEAIFDSIGKDTFDLDLKVIRRKGTLISYGNSSGAVPPFIINKLSSKNVKLLRPQLYGYVAERPEFEHYTKELFDLINSKKLNIDIHKVYQLKDYPTATKELEGRKTTGKLLLEIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSL5</accession>
    <name>A0A1E3NSL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Stress response RCI peptide</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14502</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020109.1">
        <property type="nucleotide sequence ID" value="XM_019160406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSL5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSL5"/>
    <dbReference type="GeneID" id="30177093"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF57">
        <property type="entry name" value="PLASMA MEMBRANE PROTEOLIPID 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133484" description="Stress response RCI peptide" evidence="8">
        <location>
            <begin position="20"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="105"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22938" checksum="9D494DF906ED3ED3" modified="2017-01-18" version="1">MCFCCCDCCCCDCLLIVLAVLFPPLPVCIKKGLCSCDFLINVCLCLLGWIPGMLHAWYIILENPDKVRVDDEERQIFIVSPSQTTHVVINKQPNQTRIKFNHPEPFLESSRHNSQNSEFNNNNLLNSNSLSPRSNTPLMKSQRSQYVASPLSRVENSYGAVGPNEGSSNYNFNYNDNDNDNNDDGEGPPSYENVMNETAKQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSP2</accession>
    <name>A0A1E3NSP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridine kinase</fullName>
            <ecNumber evidence="6">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_29665</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="6"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020234.1">
        <property type="nucleotide sequence ID" value="XM_019162603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSP2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSP2"/>
    <dbReference type="GeneID" id="30179290"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043771">
        <property type="term" value="F:cytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000339">
        <property type="entry name" value="Uridine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="7">
        <location>
            <begin position="15"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="8">
        <location>
            <begin position="247"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="476" mass="53737" checksum="1B602F9548E1E423" modified="2017-01-18" version="1">MEAEKYIPPWKSPYIIGIAGLSGSGKTTVAQKIISSINEPWTILFSLDNFYKELTPEQSAQAFRNEYDFDTPNSIDLDHLYDCVKSIKQGKKTDIPMYSFVKHKRLPDEKLTIYGANVVIIEGIYALHKKELLELMDCKVYVDTDLDICYSRRLLRDIVHRDRDIQGVIKQWDSFVKPNAVSYVLPTRANADVVIPRGSDNTVAMEILIQHLRRQLDQKSIDHVNHLKTLGKAVVPVNWENVHFLKPTNQLKAIKTIILNRNSTNDDFVFFFNRAASILISEALDLVEYIPGTELKKTIVTPTNCTLEMGDAFFPGQEVIAVSVVRSGDCFIRSLRRIIPAAKIGKLLIQTDSRTGEPKLHTEKLPPLEAKEGGKPSKVLLFDSQTISGAAAIMAIKVLLDHNVQQDQIVLVTYTSTETSIRKIFNAFPRIHLVVACVGKRSHESTLISNPEADSNLDSDWWMSSRFIDSRYFGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSS1</accession>
    <name>A0A1E3NSS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_84520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019736.1">
        <property type="nucleotide sequence ID" value="XM_019165007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSS1"/>
    <dbReference type="GeneID" id="30181694"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="98" mass="10507" checksum="BFE6AEBC533920F7" modified="2017-01-18" version="1">MRLCPEAKKNCPSEPICSRCAAIVLNARPPGLSPRRVWLLLAPAPRNPEAGTHGLALTTTGQTTAKAVRAAWSVLVRCSAFGPGGRQACRRSEREEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSX5</accession>
    <name>A0A1E3NSX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-rRNA-processing protein PNO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pre-rRNA-processing protein pno1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_29422</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020285.1">
        <property type="nucleotide sequence ID" value="XM_019162591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSX5"/>
    <dbReference type="GeneID" id="30179278"/>
    <dbReference type="OrthoDB" id="1932641at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042255">
        <property type="term" value="P:ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22391">
        <property type="entry name" value="KH-I_PNO1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22392">
        <property type="entry name" value="KH-I_PNO1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000009">
        <property type="entry name" value="RNA-binding protein PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055212">
        <property type="entry name" value="KH-I_PNO1_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="10">
        <location>
            <begin position="136"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="231" mass="25766" checksum="F07A5B13E1463900" modified="2017-01-18" version="1">MIDTNINEEQHEQGTNEQNPSKVRKPSAGLVMDETGKPKFQSQADSGSKVKLETRKVPIPPHRMSPLKAAWPKIYPPLVEYLKLQVRMNLKSKAVELRSCKKTTDPGALQKGADFIKAFSLGFDIDDAIALLRLDDLYIESFEIKDVKTLQGDHLSRAIGRIAGKDGRTKYAIENATRTRIVLADSKIHILGGFTHIKMAREAVMSLILGSPPGKVYGNLRTIASRLKERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSY3</accession>
    <name>A0A1E3NSY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_14605</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 11">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020229.1">
        <property type="nucleotide sequence ID" value="XM_019160455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSY3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSY3"/>
    <dbReference type="GeneID" id="30177142"/>
    <dbReference type="OrthoDB" id="5334845at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000009">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="12">
        <location>
            <begin position="4"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="10">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="442" mass="47312" checksum="CAE64D1A66344CB4" modified="2017-01-18" version="1">MSQSRRVVVTGLGLVTPLGTGVAHAWKKLLSGKSGIISTSELADADKYKDIPAKVVGKVPVSETHTSKTLPGVFNPAERFSTHELRRFSSFIQYALVAADEALMDSKWNPQELEEEDRLRSGACIGSGIGSFDDAANNAVNFATYGYRKIQPLFIPRLLANMASGNVSIKYGLKGVNHSVSTACATGLHSIGDSFRFIRDDYADVIVCGASEAAINPVALGGFARSKSVTVRFNDDPEKASRPFDKDRSGFVLGEGAGILVLEELEHALKRKAPIYAEVTGYGLSGDATHITTPSKEGDGARRAMEMAIRRANVDAADIGYINAHATSTLLGDRAENFAIKSIFEEVNPNLRVSSNKGQIGHLLGAAGSVEAIFTILALKGGIVPPTLNCEDPGTSDEDVKTDFIFDYVANKPQEIELKHALTNSFGFGGTNSSLCFSKYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSZ1</accession>
    <name>A0A1E3NSZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_23190</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019901.1">
        <property type="nucleotide sequence ID" value="XM_019162301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSZ1"/>
    <dbReference type="GeneID" id="30178988"/>
    <dbReference type="OrthoDB" id="26491at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000002">
        <property type="entry name" value="Exonuclease 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF65">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN DIN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="10">
        <location>
            <begin position="138"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="320" mass="36496" checksum="C6F0366572CCB391" modified="2017-01-18" version="1" fragment="single">MGVSGLLPVLKSIQEPTTLERFRGKTLAIDTYAWLHKASFTCAEDLVLERPTRAYINYFKKKLDMLEHFKITPYFVFDGDYLPSKGTTEKERESRRIEYKKLATEAKSGGNLKLAFNYYQKACDISPEMAKSLINELKMKYIKYVVAPYEADSQMVMLEKLGLVDGIISEDSDLLIFGCKTLITKLDDKGQCIEIRRDNFKNCKGSHISTFNDGQLLLMAAISGCDYTKGIPGIGMQKAISLVKYYKTYERVIMSIKVEGKSIPATFDEEYKRAKIAFRHQVVFNPTEQIAQHLNALPEEVTNIYSQEYIHSCTGFILDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT92</accession>
    <name>A0A1E3NT92_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020442.1">
        <property type="nucleotide sequence ID" value="XM_019163758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT92"/>
    <dbReference type="STRING" id="763406.A0A1E3NT92"/>
    <dbReference type="GeneID" id="30180445"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="880" mass="99950" checksum="DB079A0F366E3E1A" modified="2017-01-18" version="1">MEDYLNRPNVPYFLKPLSSTTVYTKVDLSDPAKYNGKNYKYHQYFANLNNNISSESTFYPNDSFAYYKTHNVFRPVNKTLTKKLDSVKNNKDQRSFQSFDSFVDVPTLFSDEQMAGLSYLTAVDMVDGVYMFGGLQALLTDEYEQKLRSITKNFTIPPENIKMVCDYDLPLPLDREKIESIAVRPYNKIRKYLTESKSFKFVCDLNDNHTAENIDEYEAENENPHLHNGHHNLKLDAFGSSNSITKTNTSSNSSSYSNATPRSMICSSACRLSDRFFMIYGGLEVGTKISYPDENHCVIKKILTPNDQFWLFDRIKCSFREIKLSVHPTYSTIFPNSIPRFAHSVASVPLDDDNIVNRNSLDDAHFFNSTHSQEDAARHSQSSTYIKPTTMFVMGGYKLHSSGKSFVAMNDLWKLDFFLDANGTADVALASPIGNFDVANDVFSYIIDEKLNHLANRKNTPKFTGVLSHSDNSSHWPSPRGFFSMCLLESCDLSKFINWRKNDKDDIAVQDSTYALLHQPSPIRKSALNQRFSESNRKITSNSSKTDFQIKDMFKKRSETAASASSVKSLSTGNEQNQSAASSTRDANNSPSPYSSRSSHSENNSHTYKNESLRGKLLLVSGGSSILYTKVVDEKNFDVYYNNNMLSDIWIFDFQTEKWYNFDDFSKLENRLFICAHTMLMSGSSLKLLGGIEEGHYPETTFLPIQAGMYDVGDDDTKVQIMDWEKGKDIVHRFHSARGAARDAYTFLNDSSIPKGLSKDGSSQECYNYYSNYTFQLESLEWRLIKTAIGQDAEFFGPWTPNKNAKVVCGGMVDDTSIREKFNLKNMNLVYAHCAAFIKDSKIVIFSPDIKIIDRKTNKYLEGSQGLVGNGVYELYSVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTB7</accession>
    <name>A0A1E3NTB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_57435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020498.1">
        <property type="nucleotide sequence ID" value="XM_019163237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTB7"/>
    <dbReference type="GeneID" id="30179924"/>
    <dbReference type="OrthoDB" id="3997287at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="557" mass="64466" checksum="412FAD1316EB5684" modified="2017-01-18" version="1">MTTGLLPSSPTFPETHDSSSSLDTGSTSSYLHNDCLKYHSSLDTVHAKIYRYNSFPELIQHLKITLRINLPRIDYPSNEILLMILSGTQLNHIEEKSSNGLLNLSVPCIEYLNQMFDIFENPSIFNDADQQRIYDEEVLNLYRKLVTLFSFLKPPIDSRRSKRKKNILNNTPQVLSLDDSLLDYSMEENMNTLEHELRNDKMQSLFEISTTSDKFNNADTFFGIKYLPEVERNLWNYFIFAFSTVSKLTEIERVDSYESYTETWVRWREFLNIILRFMELELKKSADITNSLLVLNLINISGCTDFSEMEQDQILDSLLTFCDYVFTKSRSSCSISSIIPLDLTLSQKYNFSNNPPMFEYKFQGSGIGLDSLPTRSRLLRICWKYLSYLPIDGTVKSKICGKVARKSFRLNPRELLAFFSDSYFTLDTKLVDNDFFFIVSFHVMRLISRSWSITPEVCLRDYKAYFGQLKILFKSAQSNSDQALKAKLVKGGEDFSSLNSSNEILEKCYILARYQLKVLLQSIKLEDGEMQILDEISFTVGSTNIFLGELARRIYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTB9</accession>
    <name>A0A1E3NTB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_9317</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019916.1">
        <property type="nucleotide sequence ID" value="XM_019165086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTB9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTB9"/>
    <dbReference type="GeneID" id="30181773"/>
    <dbReference type="OrthoDB" id="3998221at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF12">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="156"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="484" mass="53871" checksum="7E49A6046300D40A" modified="2017-01-18" version="1">MSYVYYPHTNPNTKTSSNLNQGCAVPLTMMTHKLATHRNSPSPNQNGFFQHPAHLHQPTSHLYTHPHHHIQQPTHLQSLPSSTSYIQMANNNQGVSFSLYPIPHQKINSFVTPNAAALKPLRKRAFSKRSKTGCLTCRERRIKCDECRPKCKNCEKSKRSCKFPSSEQSKKKKQPIKKTRPSPKKAKSIDNMALSAEASKPSSIEWLPSEIRNINISFNESAIANSSADSIICGDLLSNNQLSYQAPKPQKNSLDQKVILPPINPYVPMPKLPPMTNTHPSVQYQAHQFATLPQYDSSATHNALNNKSASVDKNMYQFENHLPYTMNISNVLASPAVQNSSLVSNNPRLIDYNYRKEQEKSSFSAIASSSSSPLFSTSGSSLSTISTSDPIQLLYPQIKYNTTVANRITPTSLSSGSDFINSTDDNTVNAPSNRFRNASVQTYYQGNSPSPVSYWPQYVYPAVNNIHVKKVSPSLPKEKGMSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTD8</accession>
    <name>A0A1E3NTD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70959</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020530.1">
        <property type="nucleotide sequence ID" value="XM_019163783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTD8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTD8"/>
    <dbReference type="GeneID" id="30180470"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF11">
        <property type="entry name" value="AMIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="78"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="696"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="729"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="858"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1046"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="858"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="868"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="896"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="916"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="943"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="982"/>
            <end position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1099" mass="121077" checksum="D4EAF6AAB289861F" modified="2017-01-18" version="1">MVESWKSIVEKKRAALASAIPKEWVKPELKEDMISKGFVKTRDYLDTILPKEEIEITSKTIPELSKLISTNELSAVQVTKAFTHRAMLAHQILGCCSEIFVDQALKRAAELDETFKETGKTVGALHGIPISLKDQVDLAQIPSSIGYVSLANQKKTQNALLADKLLEQGAVFFAKTCVPMAMMAPETASNLYPYNYSAANINLSSGGSSGGEGSLIAAGGARAGFGTDIGGSIRIPATYNGLFAIKPSVGRVSYLRVSNSYEAQESIPSVIGPLAQTLEEVEFMMETVVGAKCWLDDPKVLPLEWRKYVIPDKVKIGVWFDSGDVEPMPAITRVLGELFEDLSQAPGVEVVKVQWPDHSRLIKALFNVFGADGGKEILDECKKSGEPVHDLLDYLVGSKSPQPALDINGWWNLCQEVYRIKQIYLHFWKDNGLDAIVAPVMASTSVLPYSSVCLDYTGVCNLCDCSAVVLPLGTVDQKLDVLHPRESRSELEAKIRGQYDPKTFDGMPVCLQLITRKTEEEKGLALSQLVKTSSRFYTYNDGLQRKHIVHVPREVNPLYRMTDPSIRLRNAIKEDNYFIASRLLKRFPGLLDNIDPSNGWSNLHYSAYHNNYQISELLLKGIHNRFVSTSIRFAEQDSPAKPKSSAMYSQITDEDEIKLTFEKQTVLHVACLGDAAATLNLLLSYFNVCLDQRDMKGDTPSHICCTQGFSDCLSILLEKGAYPNIQDNNGDTLLHKAFQYSNIKCLELLIKYNADDQLLNNVGWKPVDVAFNTDIIQEYKTLKSGSVNRVPTDTVLVAKTPQTKYAPVKVTQNNVSSISFIPSNHNSSTANYPPRISLPSIQPRKYSLSSMLSDEYVDRFDPHDDTRSSSSSKNSRTSSPTGTSSPHLFMPRKTPQAGTNESNHQVTNKRYPQPPAISSSISSNGQGSTVSSPRRSNASNNSMQESPTSKRSSLSTRTFRSNSQLSEVSPLKGEHPQLVQQSTGSSAIKSGSSTPRHSFQGGYRSNSSYPVPLQVEEASSKLHDLKLKNVDRLKLDTKLGNIINNTSQENLVTSPATSQHHGGSSATDSNDNKSTSLDLSKRSKILSIPILSSRGRHNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTN9</accession>
    <name>A0A1E3NTN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger ZPR1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_70507</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a protein folding chaperone for elongation factor 1-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020026.1">
        <property type="nucleotide sequence ID" value="XM_019163661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTN9"/>
    <dbReference type="GeneID" id="30180348"/>
    <dbReference type="OrthoDB" id="308464at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000002">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000003">
        <property type="entry name" value="Zinc finger protein zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="10">
        <location>
            <begin position="68"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="10">
        <location>
            <begin position="321"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="486"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="533" mass="59530" checksum="685D57952D646CB7" modified="2017-01-18" version="1">MSQESENSANSETTSAQAVTNSNKTKQENLFQSVGEIAAEADREEDDQGLRKTGAEDAQGHAVQEVDSLCMRCGETGVTRILLTSIPYFKEIVLMSFSCPHCGFKNSEVQPASEIQEKGARYVLKIENQEDFNRQVVKSDSCSCKFVELDIEIPPQRGQLITPEGLLSEMIENLQVEQPQRKILQPEIYQKIEDLIEKVQKYVNCDPAVLPVTLVVDDPAGNSWIEYIPGEPQHKWSCTHYARSPAQNVQLGLISADEVAYREQQIQKQKAAEAQKLVQHVHAHPGDQHKEEKNDNPRATGIISDNSEIENFSNEVETFRATCSGCYSPCETHMKLVNIPHFKDVVIMSTVCDHCGYKSNEVKTGGEIPDHGTKISLYCDDSEDLARDILKSETCGMSIPELNLDLTPGTLGGRFTTIEGLLRQVRDELHSRVYTQTSDSMAASTKSRWTIFFDNLDKAIDGKTKFTIHMTDPLSSSYIQNVYAPDPDPNMKIEEYPRTAEENDELGLSDMKTENYEENSATVDAGAEVKSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTX3</accession>
    <name>A0A1E3NTX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_21857</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020603.1">
        <property type="nucleotide sequence ID" value="XM_019162226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTX3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTX3"/>
    <dbReference type="GeneID" id="30178913"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="9">
        <location>
            <begin position="103"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="338" mass="39390" checksum="A7B9AAD0DF8947B2" modified="2017-01-18" version="1" fragment="single">KMTIWEKVKHEAHHYWEGTKLLGLEIKISSRLLLKMTAGYELTRREYRQLQRTTADVLRLFPFAMFIVIPFAELLLPVALKLFPNLLPSTYESQGDKDKKLALLRNTRYKVAQVLTNSKQMVNVTSTLSDEQIADFKDFLIKLRNSKSSEIKRDQLLRVAKMFKDDLILDNSPRGMLVALAKYINLRPYGTDQILRYRIRHRMLKIKADDRLIEDDGVDSLSIPELQQACASRGIRMFNATPGQMRQYLNEWLDMRLHEHIPSTLMIFVNAYTYGDVESYPSTHEALKAVLSSLPVEVYHEQELQVDMDNATIEQKLKVLKEQEHLIQSENVQEKDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU07</accession>
    <name>A0A1E3NU07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 13, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytochrome c oxidase polypeptide VIa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_70623</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the cytochrome c oxidase subunit 6A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020156.1">
        <property type="nucleotide sequence ID" value="XM_019163691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU07"/>
    <dbReference type="STRING" id="763406.A0A1E3NU07"/>
    <dbReference type="GeneID" id="30180378"/>
    <dbReference type="OrthoDB" id="5947505at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.95.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.95.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001349">
        <property type="entry name" value="Cyt_c_oxidase_su6a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036418">
        <property type="entry name" value="Cyt_c_oxidase_su6a_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02046">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81411">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="131" mass="15164" checksum="BCE71FE437CC087D" modified="2017-01-18" version="1">MFRTVAKRQAKVIGRRFQSNLDKPAFTADPAKGAEFAKHLEAVEHHADASSATWKKVSFLLAAPIIAITAVNTFFVEKEHAEHREHTKHLSDEEWPVPKYQYLNVRKVDFFWGDGDKTLFWNSDCNRHIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU10</accession>
    <name>A0A1E3NU10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Manganese/iron superoxide dismutase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020685.1">
        <property type="nucleotide sequence ID" value="XM_019160630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU10"/>
    <dbReference type="STRING" id="763406.A0A1E3NU10"/>
    <dbReference type="GeneID" id="30177317"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="196"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="351" mass="38615" checksum="8E3685B46CC17860" modified="2017-01-18" version="1">MLRTARAQVLAALPGRRAFTHTAVRGQVSGASSRSSGSDEQALLLEKVQLPQIDRLNAFKAAGAGIPGLLSQHVLEDVWSRQLESNLGQLKEAIVQSTSNNYLDCFDSANSSIDRGADSSNKIINQYRDLLQKISNKFDDDESYLFDLTASIYNLFYFLSSLKANPANTVAKASASALLASPGEAVANAPSNEVFVSLINKSFGSMDEFLTLLSDSANSIKGNGYTWLIYKHIENSVTFSKLGHLSILNTYNNGLPHTFTSNRVSSGRDFENKKLNKNYESEKFSSNKIPSMEDADALNSLNFTFTPLLAIGSNPAFYLRDYGVYGKQQYINNVISCIDWDIVNDRIPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU85</accession>
    <name>A0A1E3NU85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_71100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020669.1">
        <property type="nucleotide sequence ID" value="XM_019163826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU85"/>
    <dbReference type="STRING" id="763406.A0A1E3NU85"/>
    <dbReference type="GeneID" id="30180513"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040049">
        <property type="entry name" value="Ribosomal_mS25/mL61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="5">
        <location>
            <begin position="57"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="155" mass="17954" checksum="9AAE73F842C3FB60" modified="2017-01-18" version="1">MPYQKIPKALRPLYLKGLPESRTLKQANHLDLIANYKNSAYKLADVEKLQLVFKRHNAYGHMGMRQFWKYNLKTISFHNPELPIEVQRVECETKDEQLKCPCLIKAHLKGGKVETIQAKDKHSDDIMKELIKATNAQKIAHEEIPLVLPKVQDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUC1</accession>
    <name>A0A1E3NUC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="17">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_14645</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="17"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020286.1">
        <property type="nucleotide sequence ID" value="XM_019160467.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NUC1"/>
    <dbReference type="GeneID" id="30177154"/>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035822">
        <property type="term" value="P:gene conversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000005">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 17" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 17" id="KW-0408">Iron</keyword>
    <keyword evidence="13 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="19">
        <location>
            <begin position="1543"/>
            <end position="1924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ49173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2256" mass="260258" checksum="450CF4C8951208EC" modified="2017-01-18" version="1">MASRGNFRGSTSARFVKNRRGNKNYSSSGQRSGPQYQQNYNNFSGSKGQNKENGNSVFDDIANERIKHVREIDLLDSIFGFDKYESGPKRTGWLVNMHSTLIQDEDSHDQLSGVDYYFLDEEGGSFKVSYQFDPYFMIACPPGKSTELEEWLRRTLDSTVKLISTIEKEDLDLANHLTGKRRTLVKLSFNNITDLLNARRTLQPYINKNKARKESNDIYKSMNLNYSSINKETIENDDLLMDVDIEEEFGFKYTDASELMLDIREYDVPYHVRVSIDENIRIGKWYTVEASPTGPNIIENTEKIAFADPVVLAFDIETTKAPLKFPDSSTDQVMMISYMIDGEGYLITNRSIISEDIEDFEYTPKPEYVGEFSIFNETDEKALLQKFFEHIRDVRPTVIATFNGDFFDWPFVEARAAFHGLDMFKEIGFAKDAEGEYKSTYCAHMDCFRWVKRDSYLPQGSQGLKAVTTAKLGYNPTELDPELMTPYAYEHPQLLSEYSVSDAVATYYLYYKYVHPFIFSLCTIIPLNPDEVLRKGTGTLCEMLLMVQAYNENILLPNKHIDPPERFYNGHLLESETYVGGHVESLEAGVFRSDLKNDFNIDPTAVDEVVADLKNSLTFAITVEGGKKVEDVTNFQEVHDEIKAKLLLLKENVKRHETPSIYHVDVASMYPNIMTTNRLQPDSMKSEEDCASCDFSRPGKMCDRRLTWAWRGEYYPAEMNEYNMIKNAAYSETFPPKYPSGPKRKFEEIPYTEQVAHVKQKIADYSRKVYHRIKSTETVEREAIVCQRENPFYVNTVRSFRDRRYDFKGLAKVWKGKVKAIESSDKHAIEEANKMVVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLTGATIIQMARQLVQRFGRPLEIDTDGIWCILPSSFPEEYNFKLKNGKSFSGSYPCSMLNYLVHEKFTNHQYQTLTDPTRYNYEMKSDNSIFFEVDGPYKAMVLPTSKEEGKGLKKRYAVFNFDGSLAELKGFELKRRGELQLIKNFQSDIFKLFLEGDTLEKCYQTVANVANRWLDVLDTKGKMLEDEDLIELICENRSMSKKLAEYGNQKSTSITTAKRLGEFLGEEMVKDAGLACKYVIAQKPIGSPVTERAIPTTIFSSEADVKSKYLRRWLRDPSLTNFDPRDIIDWEYYRERLASVVQKIITIPAALQGVRNPVPRVAHPEWLQRKIDIGDDKLKQSSLNSFFKKAPKGSKLTLPSADIEDIGLSASSNGQKVAKVTSRKRGFKNDNDLEEEEQRILKGKMPDPLVNYPAFLIYQKIKWKMKAKERERREKIFGRGNLTTLSSFSMNRMLKDQAESYLNSIWQVIEYKYDPLRPDSLKVFVLINSKLQTIHIQVQKKIYINFKNNMEFPKDEIELCEVTKTNSTLPTGSSETKNVYKLVMPHFVYEEEMKKTDGLLNSPMVEGLYETHIDAAERAIMDLGTCIKFEESKAVHLVKSFEKGFDQNHLKTLPSDGYLSKMSMGVLYLLHIVSNSYEIFTLLNNSNDTAFVYILKPSSGAQQLPSNFESMYKEVYKEKKEIVDKLYSIIDYKSEMNFEIKYFTDVSKIYKSLNSKLINITANLASKMVLVIQSHIATKLKQMFKVSVDLPTITMGISEVQVQALTWHSVIIKRIMNHYLSLSTWLKKLMQLSKYTNVPLCNLNVNNIGYLIDINYSRRLSNNNIILWWSPNSNPDYGGAEKEDFMSSIEESLKFPVFNRPEIYDTVCLEIAVKNLTVNTILSSALINEAEGVFISENGDNQNSTFADNSFSGPALTILRSMIQSWWDDAIKDVNEADALISSLLKWCSNSSSYLYNSSLHYHIHNLTKKSMIQLLSEFKRLGSSIVFADRNKVIIKTSKTVVDNSYAYGQYAISSIRAKPLFNLLELVIVRYWDMLVWMDKYNFAGSFAWEIYEDNLTQKTKLLEEKNKEENDTNIDKQSDKLEYVSKWQIKNYLPIILHQEFEDWVLMILDALIKNRKKQAEENSGSGSQVRETQITQILMQQKNLDEEVDIEKISREGLCSHFRKPLLKRINTLVGKQTDVMRDPEMAKEYEFPILAGSHLKLTNPTLELVKYICQVLSLSKKRNMEVRMLRRDLLKLFGIKEFSEEGMFKNPSTSLVIPEVICDTCYQIRDIDICKDDGQLLWHCSNCDKEYSKLIIEENLIYEFNKLLVQYFTQDLKCNKCGSMRSDELSKYCDCSGRWVNTVNVRELEKKFKIFLNVSDAYQLGLLREVVSEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUM9</accession>
    <name>A0A1E3NUM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">4-nitrophenylphosphatase</fullName>
            <shortName evidence="2">PNPPase</shortName>
            <ecNumber evidence="2">3.1.3.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70897</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57917"/>
            <dbReference type="ChEBI" id="CHEBI:61146"/>
            <dbReference type="EC" id="3.1.3.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.41" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020461.1">
        <property type="nucleotide sequence ID" value="XM_019163765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUM9"/>
    <dbReference type="STRING" id="763406.A0A1E3NUM9"/>
    <dbReference type="GeneID" id="30180452"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="305" mass="32754" checksum="98AD8D4BC4ABA445" modified="2017-01-18" version="1">MTVKITTHEQAKTLIETHDTFLFDCDGVIWIGDELLPHVKETIGLLQALGKTPIFVSNNSTKARTDYVAKLAGFGIGGIETSQIINSAYSTAIYVHEVLRLPKDQKVWVLGQSGITAELQLLGYETVTFDDLEAAAAARGAAPLAMEDVHELVDPGVGCVVVGLDFQLTYLKLGVTLQHLLDGKTPFIATNIDSTFPFKSTRLPGAGAIVETVAFASGREPDAICGKPNAGMMDSILAHHRLDKTRTVMVGDRLNTDMRFGSANGIDTLLVLTGIDREASVAAGHDDVSFYANALGDLYTLYNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUV4</accession>
    <name>A0A1E3NUV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="7">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14895</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvP family.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="7"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020551.1">
        <property type="nucleotide sequence ID" value="XM_019160587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUV4"/>
    <dbReference type="STRING" id="763406.A0A1E3NUV4"/>
    <dbReference type="GeneID" id="30177274"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000007">
        <property type="entry name" value="glycine dehydrogenase (Decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="53"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="522"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="829"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1021" mass="112472" checksum="B5B2EBBEB25733DC" modified="2017-01-18" version="1">MLRNTIRVSRLALRAPRVSVQRFQSTKVDVSTEKYASLYSVTDKYADLDTFARRHLGPQPEDVESMLNEVNSGNLQEFINSVIPESVFVGRRLKVQPAKGYSETEMLNRLKELAQKNQVLRSYIGKGYYGTKLPLVIQRNLMENPAWYTSYTPYQAEVSQGRLESLLNYQTVVSDLTGMPVANASLLDEGTAAAEAMILSYNLSRGKKNIYYIDSNIHEQTYSVIASRANTLNLDIRVADLFSKEGLDELKENIGQTCGALVSYPQSNGSIPSIKILQEISGLVHSNRGLLSVASDLLALTLLHPPSTFGADIVFGSSQRFGVPMGFGGPHAAFFAVTKALQRKMPGRLIGITKDRMDKPALRLALQTREQHIKREKATSNICTAQALLANIAANYAVYHGLDGLRNISSKIYGFTTLLANQIKDNSPHDIVNDSWFDTLSIKLNGTTSAEFMEKAVNDYGINVFKVGESNIQLSLDETVSEKDLIDLISLFTGKAITVDSLELPIFPEEWTRKDVIFTNPVFKKYHSETAMLRYLYSLQQKDISLASSMISLGSCTMKLNATVEMAPITWPEFASLHPFIPRDQAKGYMELITELEADLADITGFAKTTLMPNSGAQGEYTGLSVIKQYLHVIKGETNRNIVLIPVSAHGTNPASATMAGLKVIPIKCLSNGNLDLVDLRKQAEKYSDNLAAIMVTYPSTYGLFEPTIRDACKIIHQHGGQVYLDGANMNAQVGLTSPGDLGADVCHLNLHKTFAIPHGGGGPGVGPICVAEHLADLLPKHPLGATTNANIENAILPVSSAPFGSASVLPIPYAYIKAMGGDNIPFSSATAILNANYMMYKLKDAYKILFLGEKSQSGSDEIKYCAHEFIIDLRPFKTVHIEAIDIAKRLQDYGFHAPTMSFPIPGTLMVEPTESEDKAELDRFVDSMLAIREEIRAVEEGRSDGAILKNSPHPLTDLLETSEEEWSKRGYSREQAAYPLPHLKNFKTWPTVARVDDTYGDMNLMCTCPTVEEVANMDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVK9</accession>
    <name>A0A1E3NVK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trehalase</fullName>
            <ecNumber evidence="5">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_29282</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="5"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020696.1">
        <property type="nucleotide sequence ID" value="XM_019162583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVK9"/>
    <dbReference type="STRING" id="763406.A0A1E3NVK9"/>
    <dbReference type="GeneID" id="30179270"/>
    <dbReference type="OrthoDB" id="3542292at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011120">
        <property type="entry name" value="Trehalase_Ca-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF6">
        <property type="entry name" value="CYTOSOLIC NEUTRAL TREHALASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07492">
        <property type="entry name" value="Trehalase_Ca-bi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00927">
        <property type="entry name" value="TREHALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neutral trehalase Ca2+ binding" evidence="7">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="814" mass="93490" checksum="DEBAE16F03AD4B45" modified="2017-01-18" version="1">MLDNTKQTEKSHRRKSSVKVDPYESPDLYYGPITDPSNVNSSFGRLRTRSVIAPRATRRFSSMTDDLFATLKSKFKSASGFGAQPHAQTHAQTSSPSPLESDSKSQSSSSTSSLAKNNVPPPTPGYPLSTVINRNLQSPSPSSPTIRRRASADMSSSRINSRRFFISDIETTLKELLRNEDTDQNCQITIDDTGPKVLKLGTANSAGFNQYDIRGTYMLSNLLQELTIAKRFGRKQMILDEARLNENPVSRLYRLITTYFWKNLTRQVTRDSILDMAKDTKIKTEEAKFPRIYIPFSEKEQYYYYSKISEANKEFNLKVEYLPEKITDEFVKSINHKPGLLALAVRHRSNTYGDLEGYPYVVPGGRFNEQYGWDSYFETLGLLSCDILEPCVGMCENFIFEINNYGKILNANRSYYLCRSQPPFLTDMTLKIFDYILAKTNKMELDFLKRAFLAAIKEYDEVWCSPPRLDPVSKLSTFHPGGKGIPPETEASHFNAVLSPYCEKYNLTFTEFQQKYNDGLIEEPELDEYFLHDRAVRESGHDTSYRLEGICANLATVDLNCLLYKYETDISFVIKTYFNNKLEYNNRIETSEKWEKLAEIRKNLIDKYMWNEEQSLYFDYNIKIQKQSPYESVTAFYVLWAKVCSPERANTLIRNSLHKFEEFGGLVSGTEKSRGDIGVSKPSRQWDYPFAWAPHQILTWDGLKNYGREDIARRLSYRWLYLMTLSFVDFNGIVVEKYDATSERQPHKVEAEYGNQGSGFKGVATEGFGWVNASYLIGLQNLDVLSKRALGMVTSPTDFFAKMNPSEKDKYGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDQ7</accession>
    <name>A0A1E3NDQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015365.1">
        <property type="nucleotide sequence ID" value="XM_019160112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDQ7"/>
    <dbReference type="GeneID" id="30176799"/>
    <dbReference type="OrthoDB" id="3997036at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="450" mass="49870" checksum="D50DD6D3AAE1D2EC" modified="2017-01-18" version="1">MSEQQRVGHRSLAARHAATLKSRDKAKDKENITTTKKAAASIADSTTGLNALAHASNGRRVLRSKSANGLKVSSSSSAAASSNIFGLKESQLQPSLASTTTTSNSSANKARRVLKQPFSIFDSRDTQLNGDEAQSHHDSVTLQRSQSQIDKTNTLSRSSSALGSSFGSSLGSSINFKSSLGQARKIRSQLTIGVENDLADFFKKSKAQDQKQFGITNEDLPTNNTSENLQEITFTDEPDDITSMQRLLTERDSPLYERTHKVSVQALLSSSTGSDDIEETTFDYKDRDPKSEDGKTFMDLESLMMTTRSDNVKKEEKEDVELEIVSHNTNGIYNEEIPDSMEDMYAPMNTNLLSNLWGKPTASSLAAAEEFKSPMELDGAWRKDNLQDPKVCLEVGADEMKEPEDLLLEFSEDELLEFGDDNDDDEERKKDDKKLVEIMEFYQKERPAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE76</accession>
    <name>A0A1E3NE76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015553.1">
        <property type="nucleotide sequence ID" value="XM_019160090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE76"/>
    <dbReference type="STRING" id="763406.A0A1E3NE76"/>
    <dbReference type="GeneID" id="30176777"/>
    <dbReference type="OrthoDB" id="10253744at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF7">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="269" mass="30230" checksum="D7BB3A5CE60517CF" modified="2017-01-18" version="1">MFTSATTRRYVLTFSRQCRRFKHTTFTEKCPKPTYDTGCTFCTPPPEIQATLKTPPESIRHTIPPLSKVIVYRSNNKDNESWPKKVEAYDIMRNISKVGRGNGNMICLSSLSPKHPEAIETEADFFIFPDAKNIYIDLQSQSQDTAFVKLFQTLNSGKALESTPTLTVTPVEKTIVLICGHAKRDIRCGVVGKLVHDEFVQVLSQEKLSDKIELGYISHVGGHVYAGNVVILKPDGTVTWYGMVQPQHVQGIVEKSIKDNQVIEELSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEU6</accession>
    <name>A0A1E3NEU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_13313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015757.1">
        <property type="nucleotide sequence ID" value="XM_019160041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEU6"/>
    <dbReference type="GeneID" id="30176728"/>
    <dbReference type="OrthoDB" id="4075408at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="411" mass="46223" checksum="F8B3E303833B4A6A" modified="2017-01-18" version="1">MLKDISNNLVSVCEAIIADQQTVQKSSVNEYSKNGKKYKLTSEGNFIRVQGEDKHLIVAESYGDFKCNYSILASFRILQNCGDVLSEYRQLSLAIIFTSREIELNKWYDESSKTCFIENSSYDPLAVEDEALAYISNIDPDTRSQLVFLGCMILTATKINFFQTDHNVTSPTLEGYALRRIIAENCGEAALTSVDVYNALRAFSHWCSVRGIFHKLDIPNLQIHDLLIHRFKSFPQIPSWIKDSVYGRYPAGCSKIALVKKALVILSHSVYGQLVPLPKELDVDQFLLLCKDIETDPLRYHIRAGSLLLSTNPHLEASKLFTQSDKWLEYVSCLLQAVGSYHMASGSKLIISNKILKINKIKDKKAYKQMCTVVNEMKSLEHSQGQRCSGEELLGLMGGPVPHSLSSSCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFC1</accession>
    <name>A0A1E3NFC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipase C/D domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74527</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015950.1">
        <property type="nucleotide sequence ID" value="XM_019164795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFC1"/>
    <dbReference type="STRING" id="763406.A0A1E3NFC1"/>
    <dbReference type="GeneID" id="30181482"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004621">
        <property type="term" value="F:glycosylphosphatidylinositol phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029002">
        <property type="entry name" value="PLPC/GPLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23221">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23221:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN-SPECIFIC PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00882">
        <property type="entry name" value="Zn_dep_PLPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133330" description="Phospholipase C/D domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase C/D" evidence="3">
        <location>
            <begin position="28"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="783" mass="87998" checksum="F10C85BB19C6B1D4" modified="2017-01-18" version="1">MIFRFGVVLFWVLLFAKIDYIQAAGVAVHLNIASRVKFSIPDSLKGFESEYYAGSFHPDAFYNCFGLSLAAENAHWPPFLKAAVEYYVQRYASKGLENKSLKAFLYGVFTHQVADVAWHSLHSYQGLMRMISEVEFDGNFQAAHDYLDTNGDFIQLNREFENLSDTDFSALVEFYKKEWTYPTHDIVEIYRSLGFQEITKAKMELCMGRGFSALQGEVMAVLADRATNHRLSLNLQNSPLTFVLLNSYYYGGIDQIANTLAICMKELDEWFTATDIPEAWNICRPVFKKHSIENQGLGDTSRNYISSFPSKLQRSLHPHSNNLYLSSGIRNSQFGTSIKVGNFLGEPTIAISAPYEDFYGTVYLVPLKEILHDSKEFGQKLGIDGFKIAPSNWPISSSDFLHDFPSRFGDELFTWKLKGHEFLVISEPGLTQFKIFLYGSLVAILEFSSAETVLGCNGIKEWTILSKLPYDLNGDGWPDLVLGSMFSDDSSSRSQGGLVVILDGKKFYSNYCHRLKYSFNTEVPVINIDSIILQTYETPKVLHQTNQYDQFGVSFATTKDLVLIGLNSVGGVAVFDKKSEIFLGLLSNEGQGFFTSDFPERRASEKTSLYAFNGILTGSSNESEWIIVSGAGYSFDSICPLCGLAYLYKLENGNFELVTKLTAEVTEKSQNFVLSLFSSSMVKISDSLVVIGSSSFNDGRGALFELDVEELLDQNINLEEYTNIQPFFIADKGIGFTNFAYDSIEYFDHENHAYIAVSLANHFYSQFAHEGEKFSGSVLIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFF0</accession>
    <name>A0A1E3NFF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73678</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015980.1">
        <property type="nucleotide sequence ID" value="XM_019164550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFF0"/>
    <dbReference type="GeneID" id="30181237"/>
    <dbReference type="OrthoDB" id="1806at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990333">
        <property type="term" value="C:mitotic checkpoint complex, CDC20-MAD2 subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044774">
        <property type="term" value="P:mitotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902499">
        <property type="term" value="P:positive regulation of protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="7">
        <location>
            <begin position="12"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="214" mass="24929" checksum="3253F7811D4956B6" modified="2017-01-18" version="1">MEVNDERTLKLSGSSRVVSDYFEICIHNILFQRHVYPKEDFKVIRKFGLNLVYSKDDEVISYIKQITRQLHRWIVDGKINVLTMLIVLKETNQVSEKWMFHIDLVHDKSEEDPSSTSNSRNVKGVKTLEDIQKEIQLIIRQISSSITFLPEFEDPQTFKILVHTTDNFEAPKDWDDANLSTDIEGKGVESVKFDNFATGNHKVSTFVTYQTREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFF9</accession>
    <name>A0A1E3NFF9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_154586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015975.1">
        <property type="nucleotide sequence ID" value="XM_019160833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFF9"/>
    <dbReference type="GeneID" id="30177520"/>
    <dbReference type="OrthoDB" id="3994254at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="558" mass="64641" checksum="ECF76A3471C654E1" modified="2017-01-18" version="1">MPKANGDPGIDELEEWAEIENSEEIEEDPLFGNFEHDKGLKLLLSPKSSKTGQRKGKRKNQKIDTKKSKRISTGLDILQQLQVHMKDPLDKELGFSLNKEHTFRSANSSNSRDFMHLMDEVKREREIKVLLSDQDKAELRAKEVDDKIFQWIRSNVFNKFLDIENNGHKVHSNWISQLRKSLKAIVAQNNGEYDVSVINNNIIEDPQYSNFKFFTSQGAKTSQWRGVSFLFDEYSVESMVESLDVKYVEGFSSQKDDIVVNKTYKDLRNVLKALGVDDKLALGEMKPIRKMFDGKNDLPRLGCGFQVYKFIKTVEAKSCADLFSRKDVVHALILMSMDCNVQVDLKRFFYDGGYYTAKEIVFKTIKDLKDPLPMVNSILSKVFLQYCPDLWFKFISGVEITNDKKDILFAGKLILRFLSENKVNDGVEVYDSFEEAEGVEELFNIFCTFMKDMSGECFKLEDENLSELVYMKIELIKEMVIINHGVIKKWKNSTEDNDRFNELRSTILRVKNHYFKEYANNFNPVIPKCRRVLDFLYHELSGLEVGDFFVQDGSSQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFI6</accession>
    <name>A0A1E3NFI6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015461.1">
        <property type="nucleotide sequence ID" value="XM_019160077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFI6"/>
    <dbReference type="GeneID" id="30176764"/>
    <dbReference type="OrthoDB" id="3980750at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="455" mass="49645" checksum="84D038D7F7535681" modified="2017-01-18" version="1">MADREAHRRNSCHKNKGTENGAKRLRLGRKTSGVTAQAHKRNSEQKRKLSRERNMRLRKRSTGSRVRVRADDYVELPRNYRDKVVYDACGTTEALGQVAVRAADAEGAVWGSEASSDFEFDMSMPSTDEEDGEEHEPWQGEGPGEAAAEDGSVIPELDLNELIVLANQLVRNSPPSVTTGGGVGSGRSHSGRSSVANVHIRFPLIRTIHEARDVIMQYERLSGGRAGTGAGGEAGEGAGEGGDGAETTADDRAGRDGEVVWDSIVQDYSGISISGTRAYEAAAAAAAGEDEAGENVNVNVNLDNNVNLENMHRQPVWLELAGLATVTMFMFAVYRLVTNLVSISAFSENVVGDVVGFVEYINDKTYYKHVSHPTDHTSLIYKFGLIIKREMPYLPDTVWLVLTVVAFSAYTVLTSGVVLTSMLFATMCLTMCVVLRWRHLGDFILRVVDNGERCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFK2</accession>
    <name>A0A1E3NFK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17404</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015989.1">
        <property type="nucleotide sequence ID" value="XM_019161589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFK2"/>
    <dbReference type="GeneID" id="30178276"/>
    <dbReference type="OrthoDB" id="497380at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="5">
        <location>
            <begin position="110"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="176"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="212"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="644" mass="73877" checksum="D39C5C039981E243" modified="2017-01-18" version="1">MSIQNKGSKRSAEVATEKPSKKSKHVEVSDVESESANTETSDSESEDDLDKMDSSDDDDNDDSRDSDKTVAGGDSEENPDKKTSKEQHEEQRKLLKERKNKRKSGVEVERIKKLWERLRVQNPPLPKEVRDKLSNETWELCEGVLGDLVLKHDASRVVQTLIKYSSKERRDDICKELRPYYYKLATSAYGKYLLIKLLHYGSKDSRAIIIKELHGKLRKLMRHREGAYVVEDMFVLYSTAKQRNLMIREFWGSQYAYFPDENDTRSIVEVCNESAEKKKLISGNLFGTIKASIEKGSIGFQILHAAMKEYVQIFEGQEVRDFIDLVTEQFASLVHTPEGCEVACIVLAKATAKERKGLLKSLREHAEALATNEHGQTVLQVIFMTVDDTVLVQKTFLPSFEDKMDTLLVNKYSRRPFIYLLNGLDKSYFSPLVLKDINRYSELSKETSKKPEAERRSQILKHFLPSFYNAFSKQAYDMLGENMGAQFAQELLYNNEFPEISTELREKSIDLIIDCVRGDLTNEDHLINRPYTPRLLKSLIQGGKWNKETKKVDEIPDISLGLEFAAKFVSEIFDSGKDEATLVSWITNKESSFVLVALCDKFKEFPKHDVAKEFVKHLKKQKKTIKKQEESNKGAKLLAKVLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFL2</accession>
    <name>A0A1E3NFL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="3 11">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_59788</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3 11"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016040.1">
        <property type="nucleotide sequence ID" value="XM_019163389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFL2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFL2"/>
    <dbReference type="GeneID" id="30180076"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="12">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="9">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="152" mass="17181" checksum="953E05000034E69E" modified="2017-01-18" version="1">MSNTERTFIAVKPDGVQRALFPEVLRRFSQKGFKLVGIKLTFAKEELLREHYSDLQSKPFFPSLLSYMMSGPVLATVWEGKDVVKQGRALLGATNPLQSAPGTIRGDFALDMGRNIIHGSDSVESAEKEIALWFKKDELVSYKSEMYGWIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFU8</accession>
    <name>A0A1E3NFU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Receptor L-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_73809</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016124.1">
        <property type="nucleotide sequence ID" value="XM_019164595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFU8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFU8"/>
    <dbReference type="GeneID" id="30181282"/>
    <dbReference type="OrthoDB" id="536881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051648">
        <property type="entry name" value="CWI-Assembly_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018:SF3">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133342" description="Receptor L-domain domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="376"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="47214" checksum="AA3A8F9869F39657" modified="2017-01-18" version="1">MKFSSLLCNNLLLLASACYAGKFKSFSDGSNSSDSNIQPKCFKSEYNIYSAGDLAAISDCTVLSGSLHISHFDSDAIHLGSIKEIKGDFTVRNISNLIRIEATSLTSIGSVFELNTLNSLTSLSFPKLESVETMKWQVLPILTFVNLDTGIKTINSVIMSDTSLTGFGGFNVETLKTMSINNNRFLERIESTVSEVTGDLLIAANARNIKVSLPNLTWVKSMTVKDTEAINLESLQVIEKSADFSNNKFTTLHMPKLKSTGHTLSIINNKNLDDIEFDNLSEVGGGLMIIDNEKIKKIDFFPSLKSVGGAIEFHGDIRSNHFDDLKIIKGSAILKSSSDVFDCGEWMKNEVGKVVRGGKVECGSGKSEKTEVILVDESGESTQGEPGVNDNNKNVSRHESSIKQKSEANKSRFSAKLALVIAAYSFGILLQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFY0</accession>
    <name>A0A1E3NFY0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17534</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016142.1">
        <property type="nucleotide sequence ID" value="XM_019161635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFY0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFY0"/>
    <dbReference type="GeneID" id="30178322"/>
    <dbReference type="OrthoDB" id="3477330at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="638"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="740"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="640"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1077" mass="123731" checksum="33F66DD837A3E2A9" modified="2017-01-18" version="1">MKRAMPVTIKTELKEKSVKRRTFLGCITCRNRKVKCDGRRPKCHRCEKASRECLGYGFKLKFIDPMTLSNDGSLEVIQIQAESDKSNLAKRQQLDLMKFPASQTYTTFHELDIKLEELDENNFDGRDLYAGPFGLFASGNYSNRVHSTFTALKIDPRSFNQNLQLQVNDQSNHISSLANGNMLISEKLPSISAIIGMNRRDSDKSSPGSPISRILPLVPALELHHQRIKLQEASDLLCNSISYSSNPEKRNFGYSLRDGEIIPKPIWIHPRLEIDAILTYQTLVGSADAVTSSWDAMKKVIFAEKFSTSSELRNRITDKMQLQQSEIDSSLKKCINEVIRALNRGEQSTVPISSFTALLRSQRVQELIRLFVKSQPSVLILSYSGCVFDTIVIPLLYKIVGELMVFECSVGLPGDYMGKVSEHGIEFRDYCDVLKRTYCMVALSITSFSQYKVLFNEYGIYDGSLNLFKCYIAFREMSLVNLSVLIKPLILPSNNSRINVTNSNLVQRLIKVGLIKELVLTLILAIYQDSNVDIIVNYSLLYGVLESLKSHYQSSEKNDREMDEIWEWLRYLLIFFKSCSKIDFENYEINEEGFEDVRSDYNLIKRFEFNDYFEQNEFNRIEIKSNMDEKSKADFLSDSNDYEESLSNDSDSEEESDDEFFEDDTKLEIPKRLAKRPNIVDKPPRSFTVRFHFSEDQCDRGDCSSENEDNQNVDCCSGTIEEEIGDQQLKVHGFKTISEKNSSHKNFTSQEEDKKNQTNTRNSVGIQHAKAVALGKLNSDVRNGEDTHQEVKKRNVKMQQGSHQIGAQNVTPNKKGVEVNVLTGLATSQPDMSLNEREQLSHKKGKIQVPHPSSISWETIEGRPSSIELSFGIPVSLLRLIERAVKLADHKNWCLRKTIFPRNFPKFCCDLEEDLINWKLEWDLYTENRTKGGELQFHSLFHKALYHLAVSFYNTTLMFFFRLIKEIDPTLLQNHVTSTITHLEQLRDLSLRSDFLKDMKLFPPFWCFFISGSDAIAPELRHRFDEVARKMFVAGNKWIGKQIMMEIWRTENDGNNEICGGNSWLDVLKDWEVSGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFZ9</accession>
    <name>A0A1E3NFZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="23">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">PICMEDRAFT_17554</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="23"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016167.1">
        <property type="nucleotide sequence ID" value="XM_019161642.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NFZ9"/>
    <dbReference type="GeneID" id="30178329"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070867">
        <property type="term" value="C:mating projection tip membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090554">
        <property type="term" value="F:phosphatidylcholine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099040">
        <property type="term" value="P:ceramide translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000048">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000108">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 22 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 21 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 23" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="585"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1229"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1260"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1311"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1350"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1377"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1418"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="25">
        <location>
            <begin position="208"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="26">
        <location>
            <begin position="1197"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1554"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1560"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="20">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="701"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="701"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="702"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="837"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="878"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="907"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="942"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1022"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1023"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1024"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="ODQ45054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1625" mass="184058" checksum="7136118C964182D0" modified="2017-01-18" version="1">MSGNDNGMNSPFDDKYVFQDSDGPLPSPSVGYRSNLNKRHFSGYSTSSSKAGDDFEDASSMGKTPMLHQDSSSFAPSAFTQVNLKSSNIENRDLKFAEDDEGDEGLDDLGELRGNKPEHLQRNKTTFQRHRWGTQRKKDGRPLQRNKTLKRVLESSLPPQFFPKTDNDDDELKYDNTNKDEKDISHIKRDVYWNMPLPATSLDDEGRPPQYARNKIRTTKYTPLTFVPKNLYYQFGNVANIYFLTMNILGAFEIFGVPNPALSAVPLIVVVAITAFKDALEDSRRTVLDLEVNNQITHVLKGMDNPNFNGENVSLWRKLKKATTRIMLAIFRRLKNQKAIDPLAEDSAFPRKSLDSIDYRRSLQQQRYNDSSAMQESTDLNPFSDPITLENKDADDVIVNTHENEMPPFVSEYSDDYIAHPTFKKEYWKNIHVGDIVKIKNNDSIPVDIVILSTSDSDGACYVETKNLDGETNLKVKQALKCSHGDLISVDDLAKCKFWLESEGPKSNLYSYEGNLNYYANGDDERGELCNEPVNINNMLLRGCSLRNTKWVVGMVVFTGNDTKIMLNSGLTPTKKSRIAKELNWSVILNFLLLFVLCFVSAIVNGVYYDYKNVSRSYFEYGSVVGSAAVNGIISFFVAIILYQSLVPISLYISIEIIKTAQAYFIYSDVKMYYEKLDYPCVPKTWNISDDLGQIEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAYTEAYMEIRRRQGINVDEEVVVEKKGIASDKIEMIKQLKEINKGAISDTIDKDLTFISKKFSKDLSGQSGDTQKQATENFMLALALCHSVLTERSEKDPTKFELRAQSPDEAALVATARDVGFSFVNRTKNGLILNVQGEEKEYQILNILEFNSTRKRMSAIIKLPPVTPGGEPRALLICKGADSIIYSRLSDKNDKTLLEKTAIHLEQFATEGLRTLCIAQKQLNWAQYQDWQKRHKAASSSLQNREDKMEEVASSIEKDLILLGGTAIEDRLQDGVPDCIQLLARAGIKLWVLTGDKVETAINIGFSCNLLENTMQLLVIKTSGEDVEKIVPDDLKDTVMDDPGEMIKFLIEKYLEENFDMLGSAEELEREKKIHQPPRGNFGVVIDGDALKLALGDSLRIKFLLLCKQCKAVLCCRVSPAQKASVVKLVKETLDVMTLAIGDGSNDVAMIQAANVGVGIAGEEGTQAVMSSDYAVGQFRYLARLVLVHGRWSYKRLAEMIPCFFYKNVIFTLALFWYGIYDNFDGTYLFEYTYLMFYNLAFTSLPVIFLGIFDQDVDDYISLLVPQLYRTGILRTEWNVEKFYWYMVDGFYQSIISFFYPYFMYYKNGIVSNIGLQLDHRYYFGALVASIAIVSCNSYVMMRQYRWDWLSCLINFISNIILFAWTGIWSSSTNSSEFYKVAARLYGAPSFWACFFIGFLMCMLPRFAYDFACSVLYPKDIEIIRECVDKGDFKQYPKDYDPTDPNRPVISKYSQSHTLEAHISPVDFPAPGPSYRDSINGTGGSRGGTFKRMFSGKQNKFSPAIPTNPSVESLATEEIEMDFHKGHRNADGSMTSPSFSVKNKQNIDDSFEQTKRKMDLRDVDDVENRGVSLEGMRTSIELPELTNARSLLSNLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG75</accession>
    <name>A0A1E3NG75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_28005</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016225.1">
        <property type="nucleotide sequence ID" value="XM_019162527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG75"/>
    <dbReference type="STRING" id="763406.A0A1E3NG75"/>
    <dbReference type="GeneID" id="30179214"/>
    <dbReference type="OrthoDB" id="942095at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="237" mass="27204" checksum="8509424173A986AC" modified="2017-01-18" version="1" fragment="single">QRAQDYTELIKRFSALELSTVGNYKILSEIGSGAFGQVYLGYHKMIRCKVCLKKGNRTLSLTASDSTAANANNDNLMREFYYLREFRQHPHITKLYEIIFTENSVYMILEYYPSGDLFEYVTKNGHLSVDESLRLFTQLVGAVYYLHKSGCCHRDLKLENVLLDKHMNVKLSDFGFTRELPFAQHGSKSLLSEYCGTGAYMAPEIVQRKPYSGIKIDIWALGVMFYTMLTGEMPFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGD0</accession>
    <name>A0A1E3NGD0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_54802</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016304.1">
        <property type="nucleotide sequence ID" value="XM_019163179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGD0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGD0"/>
    <dbReference type="GeneID" id="30179866"/>
    <dbReference type="OrthoDB" id="29879at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12829">
        <property type="entry name" value="Alr1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000016">
        <property type="entry name" value="Magnesium transporter ALR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044089">
        <property type="entry name" value="Alr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF55">
        <property type="entry name" value="MAGNESIUM TRANSPORTER ALR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="665"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="744" mass="83695" checksum="2DE4E335DEAA8109" modified="2017-01-18" version="1">MTMTNSTSNSASSESSISVSSSNDEPSVHSTISGESILDDHRHQNQSLPIRTESRLLAASQEQQQKARTNSQCSVGSSRPLPPMPEKPSKVNINSNVDSRDLLNRMVADDEYDEYAVNSTTPAPLVIPGRRNSTTRTKSFSEQVKSLIRPREESTSGGILLGSNSNSNNNMAASDEELSHHSNNLVKIPSRASSKSTRKKTNREGNDADSDLDSHASRESQETEEDVCFPMLPEHVRIRGIDFDEIEEFIVEQREENERIAEREKELKMGCSNSITTSSEKISSAALKYIPKQLNARTGNSSSDLSDKSFKSEKDHYDSTSNKQEEEYEEADLPTRFSFFSSEVEETIHAADIPSLVGRGHPFKELFEPEDSIWWLDCTCPTDSEMKMLAKAFSIHPLTAEDIRMQETREKVELFKSYYFVSFHTFETDSESEDFLEPINVYIVVFKSGILTFHFSPIVNPANVRRRVRQLRDYVNVSSDWLCYAMIDDITDGFAPIIQSIEYEADAIEDSVFLSRENDFGRMLLKIGESRRKVMTLMRLLQGKADVIKMFAKRCQDEAASIRINGFSNNNSQAQPSADIALYLGDIQDHIITMFQSLLSYEKIFSRSHSNYLAQLQVESFYSNLRVTDMLSKVTLLGTILVPMNLVTGLFGMNVKVPGHGQSNYGWFGGIIGFIFLLVTVLVIGASYYLRYVERNASSQVFSSGISMKSFGFGRKKNKQRTGAGNGRDDAVSLPTRFTRYGDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGG9</accession>
    <name>A0A1E3NGG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Swiss Army Knife RNA repair protein HAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016322.1">
        <property type="nucleotide sequence ID" value="XM_019164639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGG9"/>
    <dbReference type="GeneID" id="30181326"/>
    <dbReference type="OrthoDB" id="3998224at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1082" mass="120638" checksum="BA06581A16654EA1" modified="2017-01-18" version="1">MSKWRGNYPTRGYHKRVSLRGSSVGSRGRGTRAPRGPRGTQTRVARGTRGRGGGRDYGQTPEYDGYHGRSYQPYDISHDYNYQETSDTQENNNEKTEENDPANVFTTVDNLEDSNHPSTSSHSKTYPTSTVPKIGSSGSAVPVQRHISELDDSPGRYPELSPVRPPLTGSKTGFQLSPRQDGGDLYLKRSKSDNQIDSHNSSSTENHKRKFVESVKPARNEVLSMKKVTIDNIISPAKLTNTLSFKNPASTSLSLSPRLISTGEKLNCHDPYKTPSTSSKQIEISFRSHKQGLPKRESVSPIREAILINNTELNNGPRRMEGSEASCKIHSLSPSCLPSSASLSIHRQESNISFRSREKENLQKSVDNEEKFSKKGKGIEIPNEQTVVQAVSPNLELNNDQTLVNNAKQNSINVPDKGINTENQVSPVFPTKNLSFSSSANKNTSEETKMNGVTLDSNVKCFNTTTVGSQHTTKGDGKESLSSKQSSLYSVKEQHFNDKKSEPFVQDKTKEKDIDTKESRLITSEGHNIRSKLIIREPISIPKIDSSKLELKIVETPQNLSVDSKEQKFENGIIASTTSQSANETRIGSEVDIVSGSTNAKQSSKKASFSGMLKMNGPHSGSTKEDIKEIKTPENQPTKQDVPAINSVNLPRSILSFLGVDDGAVYSSYKDNESVIKTFSLLQNENIPSSQANLNIFDFDHTMTSTLTPNPELYTDTTHSYLLASNENCVSWYNDVLPSLELEPKVMFGGIAPSHCKGQYCAALEDAIAGPEFENHESLKTLNYQWSKTLLYMVRNLCASQIASNVLIIDRYVKNISKLKADLDSLNEIGIKFHYVLYMPYKNHPDKPKNLTLSLLEEIYHTRFPWSRLTKTVTIFRPFPGNLDRSKLSKFRFLSTPGLDLRAVYTAKRCAYVKEPSTEVKHVYTAALNDKRFKLTMSISGAVFRLAYSSASSLINYLDSIKFLPVESSRSEYLFNFTDVFITYNKLPGKAIEDLGNTSLYDGAILQYGQAGKSLYVVQFLISTENVMWEYEAPIMIVARDRSISIAAAKKILKKMDWTLSDSSLNLKFTFFPSSKLKITQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGM0</accession>
    <name>A0A1E3NGM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_74067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016413.1">
        <property type="nucleotide sequence ID" value="XM_019164665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGM0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGM0"/>
    <dbReference type="GeneID" id="30181352"/>
    <dbReference type="OrthoDB" id="268479at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03757">
        <property type="entry name" value="proteasome_beta_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000027">
        <property type="entry name" value="Proteasome subunit beta type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="242" mass="27303" checksum="117DE59F65086200" modified="2017-01-18" version="1">MQKAVPTTEYANEIRNEPIEHRFNPYSDNGGTILGIAGEDFAVLAGDTRQTTGYSINSRYEPKVFSVGDNLLLSANGFAGDATQLVKVFENRLKWYHFNHNKKLETASAARLIQHMLYGKRFFPYYVHTIIAGLDKDGKGEIYSFDPVGSYEREQCRAGGAAASLIMPFLDNQVNFKNQMDPASHGTKKREVRYLKLEEVIQLVRDAFTAATERHIEVGDGLEILIVTKEGVRTEYFPLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGV4</accession>
    <name>A0A1E3NGV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015911.1">
        <property type="nucleotide sequence ID" value="XM_019164783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGV4"/>
    <dbReference type="GeneID" id="30181470"/>
    <dbReference type="OrthoDB" id="3997543at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="295" mass="32396" checksum="BFC5FB4B16A8F411" modified="2017-01-18" version="1">MATASSPLAATAPPETSTGSNVLKTGFLRSENKIIEEIVNVLTKPTKYGISDKDMFTVFNSSSSMLDNIVNGPKMTTTPFSVGTGGQTISTKMVDFQNLVALGQQLADKRKVINIESPEAVRDSLDTVKSVYRTLSSHVYINHVKTPAATAAVTAAAASSNEGQSSWWNMIYPSKVQSKTLDPKTPLGADSVADREHHSIPSSTNPLESHDYFPDLRLVLKNVLILFISFFKRYDELAIKVVPLGSLEQELMRNLKDLMLLLNVSILNKLIEIFKMLNPNIKLKAEQDIFIVLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGW3</accession>
    <name>A0A1E3NGW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_74501</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015921.1">
        <property type="nucleotide sequence ID" value="XM_019164785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGW3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGW3"/>
    <dbReference type="GeneID" id="30181472"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="5">
        <location>
            <begin position="53"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="236" mass="25732" checksum="55E5A76CA7C0B75A" modified="2017-01-18" version="1">MLPTPVVPTPDYSRVYEPAEDSFLFLDLFEQLHDAGHFARTSSTASRQPGELRVVVELGAGSGVVSTFIAANRIIPNAFHIATDINATALETTRNTFAHNCGAGAPAEAPFDVLRASLTDAFRPHEIDVLLFNPPYVPSEEIPPVPSVQDDASDSWLDLALVGGHDGMLVTGQVLASLNSVLAPQGEAYILFCARNNHPQVVQRFRQKNPNFSVDCVIARKCGWEELAIYRFTKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGY6</accession>
    <name>A0A1E3NGY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_13102</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016495.1">
        <property type="nucleotide sequence ID" value="XM_019160016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGY6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGY6"/>
    <dbReference type="GeneID" id="30176703"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF317">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE ECM32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="775"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="792"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="414"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="455"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1242" mass="139296" checksum="81F0EEF10C46A697" modified="2017-01-18" version="1">MSSNSDAPKGESKAPNENTIQESSKPVNNVKEAQNNRPVSKQAGDHIGLSKATSGMVETTEKQKKVEKNKKRKEKKVTQKLKAHEQTGRSTGIKLSAEKNGNQTGNLKSKNQKANEKKMTQKPEQQQPGSLRSFVPETEKSSKIEVVAGHEMEKKLRKAEKRKKQKAAKKLRSETGLQSNSITDNERGLENSAGSVTIDSKIGTHTDGSGLVLNNPLNLSLKFFDTLEFHCDCCPVEMVGYEAATNHVNEFNHQKMSVVFNSLSDSIKCQSCGETDLSNLHLILLASSSLGLCCSDCLEKNGYDPLIDAFSTQETLLKYVDNMYHLNGLSCISCNSTNVLGIDEEKNCYCAKCVEKDVNLKGKKLTQRSDMNFLSIAFNDSRYTDSGKSVIATVQEIPPFISDKTTKSATTSPLLSTVSSSSTSATSHHTAPVLDQRHEKEAGKTKRNDKRKKDKNIEITDSKMFRDKENDPPPKSVSSSKNVPEEKVEPKKSKKLIKGKAITSNSLTKQHESHKLKNGADSLFSKNSNDLKSAGPAKHIKNSPANTQHMSILDATDLKIEDKFERLKQIVKATLPKDIRLRFDDLNEYYSYLSYSLFLEELYAQDVCTDVDFEWKSESQCIMTGPTKKWFDMYVNDDVHHLKKHPFTRDQPIFIIRKSDAGLRWTSPPEFWLAHVSSSSLAKIGKRGKSAKVKKHAVLKKTNQVSTFTLELYPWNDRKFPVKERGDQFAFLPGSNVVGRILNSMNKIENESFKKLILGKEKIKRINFHNKIGNYFNVLNESQKNALQSALNNTVTILQGPPGSGKTSTIYEIILQLLTQLHYYPILVVAASNLAVDNIAEKLMGEHKDIILRITSLSKEKEYPESHSLGSICLHNKISQVLPPNLKDIENRLKRNASQVSSSEFSKYLDACQQYGAQIVKQANIIFATTAGIAGPYLKKVKKMPVIIMDEATQSSEPSTLIPLGAQGCNKVILVGDTAQLSVFTRVKSLEMSLFQRVLENGTYENPFMLDTQYRMHPDISHFSRRKFYGSKLKDGITAENRKMEGIKYPVYFFDHQGLGASETKMFSASGEEFGFSWVNKKEVNYIERILENLIVDHHVPPSTIGVMTGYAAQRDLIVKALENNKIINPYENKTRKYVDKEDLSEKKNVTVCNVNGIIVATIDAYQGREMNFVLLSCVRSNEGGNIGFMSDKRRMNVALTRAKYSFIICGDAKCMSSNGLWKEYIEELHDKGYVKQSINDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGZ8</accession>
    <name>A0A1E3NGZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016502.1">
        <property type="nucleotide sequence ID" value="XM_019161782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGZ8"/>
    <dbReference type="GeneID" id="30178469"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="89" mass="9889" checksum="5D39984BF96ECFDD" modified="2017-01-18" version="1">MACSRTHAARKVCVRGATEKHSKHAKPAAQAAHAERSTCTRVDLLQASFPPLGNPTRENQVRTERPTLNATRTRTHACMLHARSQVRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH34</accession>
    <name>A0A1E3NH34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_74200</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016554.1">
        <property type="nucleotide sequence ID" value="XM_019164707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH34"/>
    <dbReference type="STRING" id="763406.A0A1E3NH34"/>
    <dbReference type="GeneID" id="30181394"/>
    <dbReference type="OrthoDB" id="302728at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="121"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="333" mass="38701" checksum="48DC30A6532DD990" modified="2017-01-18" version="1">MSRLTNCLGVVSQVFPRVSVTLLVTWSFWAVLFKVILLLPPFAGCSGVFFGIVLTFVWAMCLTTYYCVLFVGPGSPTEFPMLVVPGYSEDEDGNSNMAGEAMHPPERFVSHSMMVKGNGGFRFCSKCKCWKPDRSHHCSACERCILRMDHHCPWFGECIGFRNYRYFMQFLAYSEVYLVLVTWVSCWVLVQFFVNNRWSVELFSFHVLFVFCLGLVFTLSMALFTGFTLYQVLRNKTTIESYEMQRYRRRRHNVGNVFDLGWRENWRVVMGQHWWQWLLPVTYSYDVGGQCEDADLYANGLCFPLETEVASGLRLVERLERVSGELERGLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHA6</accession>
    <name>A0A1E3NHA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_28173</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016643.1">
        <property type="nucleotide sequence ID" value="XM_019162535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHA6"/>
    <dbReference type="STRING" id="763406.A0A1E3NHA6"/>
    <dbReference type="GeneID" id="30179222"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="326"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="461"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="747" mass="84594" checksum="98BE6FD9777A645E" modified="2017-01-18" version="1" fragment="single">SEEETDIEDSIPVPSRKTRRLHRLLKSSNMDSAEQNDGTTASTKLTISKKRTDSSNLTSNSHSSRSQSPKQRKTSRAGRDASGRTLLQRMCAKGNIDEVRRLIEEGQNINDADFAGITPLHEAALEGHYEITKILLENGADINIQSGQMDKDTPLIDAVSNLHYKVVQLLLTRGANPNIQNAQGQNTFDVLESTIKEYEDDNEEVPPDAKKIKKLILQYSQNFAENDSLSSDKISRERSNSIATDDEAHSLSNNQLSYPSLRKGGLNSLQERISANDVTFVLNYVSNMNGKKIPSESLLLASKLGFPDIASLLIAFGADINFKDKNGWTPLMHAVGKGHLEMVKLLLSNQVNVAVKDKKDRTALDILKENGLCETEEYTLLLAKSKEMLALEPNNSDKMDIDQEDETGSSDKAPSASASSSPAPSVRKSASKQRKKHDDEPEEKKVTPIPPTAEEIEAHRLKEIEAQKAREALEHQRLERKKLKQQEIAKRIDAIEKQREEEKKALEKLTLEKRRKEEEEQSMLLKKQEESRKKEELKYAIEKRKLIRDYYPYGLKKANFGGKLSKSEIESFLPLYVFKIGDAEYLIDLQLNLLFGVESLYSKYPQLDKRKVRLEEKKGLWNVLWPLIGSFQNGYKPVEELQQVYESEGNNFKELVLNWVRLDAFEELIKTEELRVVHESVERIGLCHAALVIETSQVSVSDADGANAVNNNTIKDIEPTDNRPREFPRRFGKKARTALKMMNKPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHK1</accession>
    <name>A0A1E3NHK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_59269</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016720.1">
        <property type="nucleotide sequence ID" value="XM_019163352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHK1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHK1"/>
    <dbReference type="GeneID" id="30180039"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="6">
        <location>
            <begin position="195"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="346" mass="38522" checksum="962CD0A24D1F2FB3" modified="2017-01-18" version="1">MAVSVPKVLLIGFGGVGTIVSYTLEHLGRAEVVAVARPITFDKIANQGYRIESVDYGTVEKYVPSKVVLSGKEAVEKYGPFDYIVITTKNIPDISPVVDMLEDCYSDDTAIMLIQNGIGIEIPIYKKYPKATVLSGVTLIGTTLYESTVKHVARDDIKFGPFINYNLSKESQIAKCKKFVEIYSNENNSTEYEENVKLTRWRKLVYNTAINTTCAIVNLDSGRVELFGGADSVLRPAMEEVIATAKSEGVTLPASLIDEMIRGADGVYYPPSMLIDTRNGTYIEHIVIIGNVVKIAKRNNVPVPTLTVLSNLLKMIQMRTMEQNGRFVLPKKRPTREDNYQIEYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHM9</accession>
    <name>A0A1E3NHM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_73168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016768.1">
        <property type="nucleotide sequence ID" value="XM_019164403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHM9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHM9"/>
    <dbReference type="GeneID" id="30181090"/>
    <dbReference type="OrthoDB" id="63112at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013893">
        <property type="entry name" value="RNase_P_Rpp40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08584">
        <property type="entry name" value="Ribonuc_P_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="352" mass="39967" checksum="8A20D7D8C870C96D" modified="2017-01-18" version="1">MKEYLVCHSTVSGGNFNATAVTKVEKLLDSHNFVNKVDLLVRGSDLEQIKSDVQAQMKLKKILGEANLSQLLGFLLRLNVEQRSALKLVTLDKDITHDNSICVTKGNLTLKLRSDSYLKSGLQCKLSAFSRGNKNLKSQMYIHNFDMLNFENNVKLNRKNEIRLLWYAENVSVDVFKFTLTSESDNIDTQLVPKLEEEGITLLGNREVESRVFRYNQVLHPDLTITKDEDTLAETLEWLSYASIGGLQLRQEVDPFISRYPTMLESSKRSDFAVLSIDKTLISSKVQKLVFSHVSSRLDWFALFSYGVKNVSRSYNSAGEHYFVDDGANDIVVFSQEGKYALWEITDSGDPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHY4</accession>
    <name>A0A1E3NHY4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_12695</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016056.1">
        <property type="nucleotide sequence ID" value="XM_019159961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHY4"/>
    <dbReference type="STRING" id="763406.A0A1E3NHY4"/>
    <dbReference type="GeneID" id="30176648"/>
    <dbReference type="OrthoDB" id="29460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018939">
        <property type="entry name" value="Autophagy-rel_prot_27"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09451">
        <property type="entry name" value="ATG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="263" mass="27740" checksum="FE90440F86C99344" modified="2017-01-18" version="1">MAVCCMEAVTAFDIGKEYFTKMRVDDAIVGVHSSEAKQVTPPSTIVSSWYAAMQKGKPSSGNGIGHGCPADSTLCGVVRLDTADEAIQLLSVREEDGKYAYDEQRGLRAEWSGVAYGDLTLTVVLDFTCVDSAEDRLGWVNHTSAFIDGDVHLAWSNKQFCSKDGRSGGGDGGKDGGKDPQDSGLGLFGMFFLIGAVAFAGYLVAQAWFNTSTMGNSSDFFNELVDIVVDSISSIPRLVLEVVNKITGGGAAGNASRGGYSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI94</accession>
    <name>A0A1E3NI94_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase GCN5</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_36485</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016176.1">
        <property type="nucleotide sequence ID" value="XM_019163010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI94"/>
    <dbReference type="STRING" id="763406.A0A1E3NI94"/>
    <dbReference type="GeneID" id="30179697"/>
    <dbReference type="OrthoDB" id="1937912at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140068">
        <property type="term" value="F:histone crotonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036408">
        <property type="term" value="F:histone H3K14 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043993">
        <property type="term" value="F:histone H3K18 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140129">
        <property type="term" value="F:histone H3K56ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140011">
        <property type="term" value="F:histone H4K12ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140046">
        <property type="term" value="F:histone H4K16ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010515">
        <property type="term" value="P:negative regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000046">
        <property type="entry name" value="Histone acetyltransferase GCN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 14" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="75"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="16">
        <location>
            <begin position="318"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ45063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="418" mass="48234" checksum="1123150C49107061" modified="2017-01-18" version="1">MENQDDASNDGSPENPAEDAAENEEDEQKPEEGGGEDAEDVDAEEKKKLHCYQFDGYTYTYKDRPSVIEEREGKIEFRVVNNDDTRDNMIVLTGLKNIFQKQLPEMPKAYIARLVYDRSHVSIAVVRKPLTVVGGITIRPFESHEFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRSTTKIKYFLTYADNYAIGYFKKQGFTKEITLPKKVWMGYIKDYEGGTLMQCSMLPRIRYLDSPKILSLQKAAILRKIRSIGRSHIVHKGLDHFKQKSFEPLNPFDIPGLKEAGWTREMDDLAQKPKRGPHHIAMLTILTEMQNHPSNWPFLHPVNRTEVPDYYEVIKEPMDLGTMEVKLENDAYETMDDFVYDCSLIFNNCRQYNGENTTFYKNANKLEKAVIAKLKDFPEYSSCLDILTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIE1</accession>
    <name>A0A1E3NIE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017028.1">
        <property type="nucleotide sequence ID" value="XM_019161494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIE1"/>
    <dbReference type="GeneID" id="30178181"/>
    <dbReference type="OrthoDB" id="3997513at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="135" mass="15289" checksum="37395861339C20F9" modified="2017-01-18" version="1">MSNDKKHTHMKDGIPQRKTANQQMKESINFDEPFQALPLQIILRVLYNLTKSRSADTKHFVTVGVMLFQAMVIERKLRPSQGCFEELLCLAMAHPPTAEQAPSIMTIYYKNHSIVGPHMSATLDSLGLDHNGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIE4</accession>
    <name>A0A1E3NIE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_36428</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016461.1">
        <property type="nucleotide sequence ID" value="XM_019163005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIE4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIE4"/>
    <dbReference type="GeneID" id="30179692"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="4">
        <location>
            <begin position="150"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="352"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="410" mass="44824" checksum="BBA0B5C5E5140B9D" modified="2017-01-18" version="1">MPGPLKKDTAAAAASLAQTAARAFQPRQEFPDYRVVLAQLKGHQTKALHRMAQLAPQINLVVELRDARAPLATANVLLDKVFKGKDKLIVYTKKDMSPVSSQLLQRWHAPRNEKWLAIDSRKNRDVALVLRALREKHRSMLPPPPLGLRLMVAGMPNVGKSTLVNELRRHGLAAGGGGGAAGGDERRFRKVARTGNMAGVTRNTSEIIRISAEPEMLLYDTPGVLLPQVDSVRTMLALYLAGTVSASSSSDGGIDPVIATDYLLYMMNLADPSGAGYRRYLPHPTNDVYELLDGVGRLTGNRNRRKIDGGKLRTNYVGCALELVKEFQQGRLGRWCLDEAVVRQLSPDAFSEQAAAERRRVDSHPSRLGKTLDPDAAATATDASAAAAKTTKAARRRERLVKQSNQLFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIF8</accession>
    <name>A0A1E3NIF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dynamin GTPase</fullName>
            <ecNumber evidence="3">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_73912</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016246.1">
        <property type="nucleotide sequence ID" value="XM_019164618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIF8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIF8"/>
    <dbReference type="GeneID" id="30181305"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030061">
        <property type="term" value="C:mitochondrial crista"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901612">
        <property type="term" value="F:cardiolipin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140523">
        <property type="term" value="F:GTPase-dependent fusogenic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180020">
        <property type="term" value="F:membrane bending activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097753">
        <property type="term" value="P:membrane bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097749">
        <property type="term" value="P:membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990627">
        <property type="term" value="P:mitochondrial inner membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990626">
        <property type="term" value="P:mitochondrial outer membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000741">
        <property type="entry name" value="Putative mitochondrial dynamin GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056495">
        <property type="entry name" value="LIS_MGM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF212">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24550">
        <property type="entry name" value="LIS_MGM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="20">
        <location>
            <begin position="188"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="19">
        <location>
            <begin position="754"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="129"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ45133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="849" mass="95631" checksum="74C87A5E1218BE76" modified="2017-01-18" version="1">MSFSNRRHISTNQLIRPFSFFSIGKAAAKAAKVPVYLGGSLAAAGSYVMYKVEEATNYTSEQLDRAKELASGAKNGFNGLLDGMLSHFGSSEGGEDNGQNGNNNNNNNDNDDDTTLVGSAAAASAIGMLGNSSDEETLQEEELLEEDDEVDEDEENKQRQILEDQMLNLTRQMIEIRNILLNVNGATDLRLPSIVVIGSQSSGKSSVLESIVGQEFLPKGSNMVTRRPIELTLVNDPSSASEIAEFPALKMFNMTDFQQVQKVLVDLNLAVPHDEAVSADPIQLTIKSPKVPDLSLVDLPGYIQVEAADQPTTLKTKIREVCDKYLEEPNIILAISAADVDLANSAALRAARRADPRGERTIGIITKLDLVEPDVARGMLTNKKYPLKMGYVGVITKIPKSTSIFQRKTGVQAFIAQQNTERMFFRENKDVFSGLSVGTKLLKKKLIRVLERSMSNALRPMQQQVLNELEETSYKFKVEFNDRDLTCEKYMAQTIDNLKMEIKEFSEKFGRPELKSLLRSELDQKVLDLLALRYWNKSEPYIKKNPAISDYEFEEPQISELSNAKNDETFWHRKLDLSTANLTKIGVGRLSTTLVTEAILTEIDVLINSTDLRNNDMIKQAIKEAAISVLNQRYNSTADQVENCIKPFKYEIEVEDREWSLSRKHSILLLNEELREVQVAYDVIKKTVGGNKLKQIMTYIENENNSVGQTIERENLGFSPGLILRGREAIFLKDRLAILNLRLKGLQSSTCKHKENKFKCPEIFLDVVAEKLTQTAILFLNVELLSDFYYSFPRELEKRLGTKLTTEEIENYAKQDPKVKRHIELQERKELLELALSKIDDVMAIQKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NII1</accession>
    <name>A0A1E3NII1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted beta-glucosidase adg3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_34357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017052.1">
        <property type="nucleotide sequence ID" value="XM_019162879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NII1"/>
    <dbReference type="STRING" id="763406.A0A1E3NII1"/>
    <dbReference type="GeneID" id="30179566"/>
    <dbReference type="OrthoDB" id="5554151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053088">
        <property type="entry name" value="Beta-glucosidase/SUN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31654">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE ADG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31654:SF0">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE ADG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133375" description="Secreted beta-glucosidase adg3" evidence="2">
        <location>
            <begin position="22"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="31759" checksum="F969CBDFAD609C1D" modified="2017-01-18" version="1">MLSIKSLSLIILSTLVSESVSAPTSLHEKRDGGNTCSFPNSDGMVAVFNTKVANSEGWAQDGPCKANSYCQYACQPGYLMGQWNPDVTSYSYPGSQDGGLHCDSNGQLSKPISNSDYCVKGKGTAKAVNKAGDKVAFCQTVLPGNEAMLIPTEVAGGSDNDLAVPGTDYWASTASHFYVNPPGVSAQDGCVWGSTDKAQGNWAPYVAGFNMDDSGNTFAKIGWNPVYFEGSSPFSNTKPSYGIRLKCDDPSKCNGDTCEINPSKYGLNKMSNQQQSNSDGAAWCVVTATGNSGVSVEVFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIT5</accession>
    <name>A0A1E3NIT5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017133.1">
        <property type="nucleotide sequence ID" value="XM_019164500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIT5"/>
    <dbReference type="STRING" id="763406.A0A1E3NIT5"/>
    <dbReference type="GeneID" id="30181187"/>
    <dbReference type="OrthoDB" id="3998110at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="441" mass="48846" checksum="1EF68CBF2E8C1596" modified="2017-01-18" version="1">MSLALELLDRRNKAFGAQSQMCESSNAAELCAFLQQEYEDYDDARYDESGCDNEFVDCYVRRLNDVVKVGRIGYSGRTQGRDESPADAVELNILIEKSLLEYELRLAEKVPRGDSGKSTEGNRKKLVFDVERLGEVLSGAGDDGSDSDLALLRHYVELLKLGYAPELAANRNGGGQNASENTLMDELVSTLLSLAVQRNVSLPPLDTKAAESMDGRIGWFKECIYKVAGAAEGPRDRNQDSGAASASRSAAGDDDENYRALRDLQFAHTYLTKKYEEEVSQHGQAMNSMVAKYQQCEDLLKQSNFELSKVSNQLLRLELQNAELTKKLDARTKDAHELQLKNNLLRVDYLGLAPATTPPASADSAEAPADSLSLSLPSPASSASSATMPSSSVSVRILRMEFKKLVEQMNARFARELETEQTEKRRLEELVELHRQRGAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIT9</accession>
    <name>A0A1E3NIT9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_74258</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016611.1">
        <property type="nucleotide sequence ID" value="XM_019164723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIT9"/>
    <dbReference type="STRING" id="763406.A0A1E3NIT9"/>
    <dbReference type="GeneID" id="30181410"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044280">
        <property type="entry name" value="Hac1/HY5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714:SF6">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0834">Unfolded protein response</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="134" mass="14978" checksum="A70900082ED3C747" modified="2017-01-18" version="1">MSSPLSDNSNFSRKLSAELIDVNSNGSHLLPRKRARAEAEKEQSRVERIIRNRKAAHASREKKRKHVEQLESYVTVLEAHLNLSLEANRTLLSKLAANNIPCDGVDIKKLQVPRPDGLLLSDEDKTSASNNVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIZ7</accession>
    <name>A0A1E3NIZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Homoaconitase, mitochondrial</fullName>
            <ecNumber evidence="5 17">4.2.1.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_34691</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the reversible hydration of cis-homoaconitate to (2R,3S)-homoisocitrate, a step in the alpha-aminoadipate pathway for lysine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>(2R,3S)-homoisocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
            <dbReference type="EC" id="4.2.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 3/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.36" evidence="5 17"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017227.1">
        <property type="nucleotide sequence ID" value="XM_019162900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIZ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIZ7"/>
    <dbReference type="GeneID" id="30179587"/>
    <dbReference type="OrthoDB" id="10262323at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER01027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01674">
        <property type="entry name" value="Homoaconitase_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000013">
        <property type="entry name" value="Homoaconitase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004418">
        <property type="entry name" value="Homoaconitase_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039386">
        <property type="entry name" value="Homoaconitase_swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00139">
        <property type="entry name" value="h_aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001614">
        <property type="entry name" value="PRK00402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 17" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="18">
        <location>
            <begin position="25"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="19">
        <location>
            <begin position="488"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ46114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="689" mass="74016" checksum="5A54F3F38CD1FF1E" modified="2017-01-18" version="1">MRVQACGRRFSTTSFTLQGQNLVEKIVQKYAVGLPKDKVVHTGDYVSIQPAHVMSHDNSWPVALKFKGLGAKKVKDNRQIVNTLDHDVQNKTEKNLTKYENIKNFAKEQGIDFYPAGRGIGHQIMIEEGYAFPGNMTVASDSHSNTYGGIGALGTAIVRTDAAAIWATGQTWWQIPPVANVVLEGELPKGTTGKDIIVALCGLFNNDEVLNHAIEFTGEAVKHLPVDYRLTIANMTTEWGALSGVFPIDETLVNWYTNRLLRVGPNHPRINVKTVENLQQNKVTADEDAFYAKTLRIDLSTLSPYVAGPNSVKIGTSIDKLSSKNMKINKAYLVSCTNSRLSDIKAAADVVKGNKIAPGVEFYIAAASSEVQQDAEADGAWKTLIEAGCIPLPAGCGPCIGLGAGLLKEGEIGISATNRNFKGRMGSKDALAFLASPEVVAASAVLGKIAAPEEVAGEPCHPATEVKKVVTVNKKPESTTDAASAEAASASASSSSVLEGFPELIEGEVIFCDADNVNTDGIYPGKYTYQDDVPREKMAEVCMENYDSEFGSKTHAGDIIVSGFNFGTGSSREQAATAILARDIKLVAAGSFSNIFGRNSINNALLTLELPDLIHKLRAKYAGSEKELTRRTGWTLKWDIPNSLVTVSNEAGDVVLTNKVGELGTNLQEIIIEGGLEGWVRAQLKQQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ04</accession>
    <name>A0A1E3NJ04_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Arginase</fullName>
            <ecNumber evidence="2 10">3.5.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73600</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="2 10"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017240.1">
        <property type="nucleotide sequence ID" value="XM_019164532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ04"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ04"/>
    <dbReference type="GeneID" id="30181219"/>
    <dbReference type="OrthoDB" id="9992747at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903269">
        <property type="term" value="C:ornithine carbamoyltransferase inhibitor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090369">
        <property type="term" value="F:ornithine carbamoyltransferase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090368">
        <property type="term" value="P:regulation of ornithine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09989">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000012">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014033">
        <property type="entry name" value="Arginase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01229">
        <property type="entry name" value="rocF_arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782:SF3">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="321" mass="34701" checksum="159B61A89F49731E" modified="2017-01-18" version="1">MSNPHYKLYPKKHVTVIKAPFSGGQGKSGVEDGPEQLLKSGLLHQIESLGWECSVADPLEGLDSAKMKANVEDVYERCKRPQMVSECCERIYKAVETCHQQGSFPLTVGGDHSIGMSTITAFVNAHPNGGIIWVDAHADINTSSTTCSGNLHGCPVAFAMGLDRKNWPPQFKWLDALKTLVKPEQITYIGLRDVDAGEKKILRDLGIKAFSMYHVDKYGINEVVRMAMEAVNPSGENPIHLSYDVDGIDPLYVAATGTPVRGGLTLREGLFIVEEVANSGNLAGLDIVEVNPSLATTDIHIVDTVNTGLSIAKCALGDTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ29</accession>
    <name>A0A1E3NJ29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_34755</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy; may act as an adapter for ATG8 that supports mitophagosome assembly. Negatively regulates the proteolytic processing of ATG32 via the i-AAA protease. Acts as a negative regulator of the m-AAA protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. The mitochondrial prohibitin complex interacts with the m-AAA protease, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. The mitochondrial prohibitin complex interacts with ATG8 and the interaction may support mitophagosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prohibitin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017252.1">
        <property type="nucleotide sequence ID" value="XM_019162906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ29"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ29"/>
    <dbReference type="GeneID" id="30179593"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF0">
        <property type="entry name" value="PROHIBITIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133381" description="Prohibitin" evidence="6">
        <location>
            <begin position="18"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="7">
        <location>
            <begin position="25"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00057247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00063239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="280" mass="30833" checksum="F771B9971048BF68" modified="2017-01-18" version="1">MSRIANAISRLALPVSALILLADYSMYDVKGGERAVIFDRFSGIKPNVVGEGINFVVPWLQRPIIFDVKTRPRTITTTTGSKDLQTVSITLRVLHRPDVMNLPAIYQNLGLDYDERVLPSIGNEILKSTVAQFDAAELITMREVVSNKIRDDLEKRASQFNIKLEDVSITHMAFGQEFTKAVERKQVAQQDAERAKYLVDKADQERKAAVIRAEGEAEAADHISKALAKAGDGLLLIRRLEASKEIAETLSRSPNVAYLPKGGSGDDGSSSQSLLLNIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ54</accession>
    <name>A0A1E3NJ54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Allantoin permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017285.1">
        <property type="nucleotide sequence ID" value="XM_019161571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ54"/>
    <dbReference type="GeneID" id="30178258"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="484"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="65290" checksum="1D55A68DE206E81C" modified="2017-01-18" version="1">MATLDWLSRLNEKIIIPNTDSKEDGIRSNKDLDPTNVEDRTWRTYNFFLVWFQSSLNVTIWNSGASLIKSSQISYWKTMIASVVATFIGGLFVMWNSRAGAVYHVGYPVLLRSTFGVWGYYFFVIIRGFVAILWFAVQTYYGGCLLDVVLRCIFGHKWNNIVNHIPPEQGITTKLMIAFLLFWICQWPVMVIHPTNIRHFFTFKALALPWATLGIFIYCCVAGHGPGNFNMGIKIIEDPSQVGWKFMMCINSILGSISAMIINQPDVARYCRKPSAPLLPQFFGFMLSKVAIQLFAMVAVASYYRVSGVAYWNLWDLLNGILDRHWTAGARTGCCLVAGAFCIGNMGTNLFGNALPFAADMTGLFPKYINIRRGQILMACICWGIVPWKMLKNATTFLTFLGSYTLFVGPILGCMLADYFFVRKGNYHTPSLYTRSPNGVYYYYKGVNWWGCLAWVFAMALGIPGLAAAIHPKAYSINCLHMNYIGWLMCTFAGMVFYTLFGKLVKPQVYPPGHEATPKTFEYMKDSNGFFDDDEPINGVGPVDFEPASFSSQSFQKDGKELAVTEVISLDQLDTTSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJA0</accession>
    <name>A0A1E3NJA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="5">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF6</name>
        <name evidence="6" type="ORF">PICMEDRAFT_16139</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <text evidence="5">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017337.1">
        <property type="nucleotide sequence ID" value="XM_019161100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJA0"/>
    <dbReference type="GeneID" id="30177787"/>
    <dbReference type="OrthoDB" id="4155914at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="modified residue" description="Phosphoserine; by CK1" evidence="5">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine; by CK1" evidence="5">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="245" mass="26322" checksum="A930426A048151B6" modified="2017-01-18" version="1">MAARAQFENSNEVGVFSKLTNSYALVAVGGSENFYSAFEAELGDVIPIIHTTIAGTRIVGRMTAGNRRGLLVPTQTTDQELLHLRNSLPDSVKIQRIEERLSALGNVICCNDYVALVHPDIERETEEIIADVLGVEVFKQTIAGNVLVGSYCALSNQGGLVHPQTSIQDQEELSSLLQVPIVAGTVNRGSNVVGAGMVVNDWLAVAGLDTSAPELSVIESIFRLQDNQPDAIGSNLRDTLIETYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJA4</accession>
    <name>A0A1E3NJA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_178719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016771.1">
        <property type="nucleotide sequence ID" value="XM_019161786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJA4"/>
    <dbReference type="STRING" id="763406.A0A1E3NJA4"/>
    <dbReference type="GeneID" id="30178473"/>
    <dbReference type="OrthoDB" id="5121955at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF4">
        <property type="entry name" value="ACETAMIDASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="320" mass="36708" checksum="FC394B9E63116A51" modified="2017-01-18" version="1">MAVKQPVANDEVSHENSRSEQDNRASKHDRSSRACLVCRQRKVKCDALECYPDKCTNCVQFNIKNCFLPAPKKRKSKKIEELLKTNLSLQNSPNSSDLSGDATFLNISTDGPYPSFVGEDIQRKHPDLAEIQLDPARVLKYIPGSVERNADFTDWIRPFMGTVLKSFLSEHSNNPTGYTKINATEYSHLVQSGCFLLPAPDVCWKWINAFFTTRLCQYPILIESTFREEYKDLTNPPSLLLLHSIIYCGAKYYDDPSWSTAERLAHIEKTEILYTRALTFYDKKIEMDLLCQLQSLLMMANYKTANDQCLRADPFLSEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJB8</accession>
    <name>A0A1E3NJB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Catalase</fullName>
            <ecNumber evidence="12">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17363</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 13">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="12"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017279.1">
        <property type="nucleotide sequence ID" value="XM_019161568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJB8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJB8"/>
    <dbReference type="GeneID" id="30178255"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08157">
        <property type="entry name" value="catalase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000001">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="7 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 12" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="14">
        <location>
            <begin position="8"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="500" mass="56615" checksum="565EAF4651536F30" modified="2017-01-18" version="1">MSNPPVYTTSSGCPVSDAFSTQRISVQNNDYKYRPPVGPLLMQDFKLIDSLAHFDRERIPERVVHAKGAGAYGYFEVTDDISDLCSAKFLDQVGKKTKIFTRFSTVGGEKGSADTARDPRGFATKFYTEEGNLDLVYNNTPIFFIRDPTKFPHFIHTQKRDPQTNLKDANMFWDYLTANPESLHQVMYLFSERGTPASFRTMNGYSGHTYKWYNAKGEWKYVQVHFIARQGVHNLNNEQAGKLAGENPDHNTQDLFDAIEKGDYPVWDCYVQSMTLQESKKLPFSVFDLTKVWPHSDFPLRHFGRFVLNENPTNYFAETEQVAFSPSNTVPSMQPSNDPVLQSRLFSYPDTHRHRLGANFHQIPINCPLKSGSFAPQIRDGPMCVDGNLGSTPNYAGAYNCPVQYTAAAKVAPDGADEQYQGEVVPYHWEICDYDFAQPAKFWDVLGKTNGEQEALVHNIAGHVQAAKPFIQERVCEYFSKANPAIGQLIKEELKKLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJC5</accession>
    <name>A0A1E3NJC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017358.1">
        <property type="nucleotide sequence ID" value="XM_019161110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJC5"/>
    <dbReference type="GeneID" id="30177797"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133388" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6361" checksum="8EABED991F8CB067" modified="2017-01-18" version="1">MPSGSPLLLYLTTCLQLHPTPSACSCCLRLPLYGGPIRGRLGPGGASAHNPHKADLFVVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJL5</accession>
    <name>A0A1E3NJL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mRNA-decapping enzyme subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16233</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017447.1">
        <property type="nucleotide sequence ID" value="XM_019161132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJL5"/>
    <dbReference type="STRING" id="763406.A0A1E3NJL5"/>
    <dbReference type="GeneID" id="30177819"/>
    <dbReference type="OrthoDB" id="440673at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21228" checksum="28EA5CA5F59279FD" modified="2017-01-18" version="1">MTINNNTSSAEGADSSQTIEIYKQALTFNVIAKYDPLISQLLHISSYCVIYQYDEEAEDWVKSSYMGPVSVYARRSVWDKNGKDDNITRVRVDNVLAAKQGDLYRFGLIVLNRSQPENFSLGFLGDKYLSQEDLDEDRGLLIEKSDQLIIVRDFNGKAFGLWIFDEKDRDYLYQLLMYCTDQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJS1</accession>
    <name>A0A1E3NJS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Glutathione reductase</fullName>
            <ecNumber evidence="3 16">1.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_16897</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 16">Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH:GSSG ratio in the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 16">
            <text>2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.7" evidence="3 16"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016706.1">
        <property type="nucleotide sequence ID" value="XM_019161386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJS1"/>
    <dbReference type="GeneID" id="30178073"/>
    <dbReference type="OrthoDB" id="5956163at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000003">
        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000235">
        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006322">
        <property type="entry name" value="Glutathione_Rdtase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01421">
        <property type="entry name" value="gluta_reduc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004776">
        <property type="entry name" value="PRK06116.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF2">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="18">
        <location>
            <begin position="5"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="17">
        <location>
            <begin position="349"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="183"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="14">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ45593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="461" mass="50655" checksum="117CF67A84ACECB2" modified="2017-01-18" version="1">MKSHYQYLVIGGGSGGVASARRAAKHGASTLLIEAKAMGGTCVNVGCVPKKVMWYASDMAAKLKLAYDYGFKNTKDLDLEFDWKTFKEKRDAYVHRLNGIYERNLTKEGVDYIYGFAKFSKDGKVEVTKTEDGKVETFTADKILIATGGTSVIPKDIPGYEYGIESDGFFDLNSQPKSVAIIGGGYIGTEFAGVFNALGTKTHMILRGKSVLTKFDSLIKETIHETYKKEGVNILTHTKVLKVEKLPSGEKKLYLTDDSTLVVEELIWTMGRKSLLGIGTENVGLKLNEREQVIVDEYQRTNVPEIYSLGDVVGNLELTPVAIATGRKLSNRLFGPEIYKDQKQDFDNVPTAVFSHPEAGTIGLTEEEAVEKYGEDDIKVYKSKFNGMYYAMSEHKSPTAYKLVCVKSLDEKVVGLHLVGDASAEILQGFGVAIKMGATKADFDSCVAIHPTSAEELVTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK53</accession>
    <name>A0A1E3NK53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="15">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_72535</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="15"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017579.1">
        <property type="nucleotide sequence ID" value="XM_019164226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK53"/>
    <dbReference type="STRING" id="763406.A0A1E3NK53"/>
    <dbReference type="GeneID" id="30180913"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF171">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0274">FAD</keyword>
    <keyword evidence="4 14 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10 15" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="41"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="294" mass="32063" checksum="989D45A7FE0751BC" modified="2017-01-18" version="1">MSKVLKFLVPAAAVAGGLYFYQTGSAAAPAALVPPALKGDGEWVDLKLTNVKPVSNDTKVYTFAFPNANQTSGLVVASAILTKYVTEKGNNVVRPYTPITDVRKKGSFDLLVKTYEGGKMSVHMRGLEVGDSLPFKGPILKWKWEPNQFKEIGLIGGGTGITPLYQLVHEILSHNDVDKTKITLLYGSKTMDDILLKEELDALAAKYPDQFTVKYFIDKIVDSRKDDKITVGYIGKDVLESNLPKPSADSHVFICGPPPLYKALSGVKVSPTDQGEVSGVLKELGYDKTDVFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKI7</accession>
    <name>A0A1E3NKI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycolipid transfer protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72687</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017744.1">
        <property type="nucleotide sequence ID" value="XM_019164272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKI7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKI7"/>
    <dbReference type="GeneID" id="30180959"/>
    <dbReference type="OrthoDB" id="205255at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000001">
        <property type="entry name" value="Pleckstrin domain-containing family A member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF25">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="197" mass="22063" checksum="F7038F4C03E935C7" modified="2017-01-18" version="1">MTFFDDMKTSFADVPRNDGEISTLEFLEASESLVKLFDLLGSSAFSVVQSDMSGNINKVSAKFLSDPVNCGTLQKLILTEVKEGGKKTATQGLLWLTRGLQFTAVAMRKCIDNQDWELSKCFEKSYGETLTKYHSMLVRPVFKLAMKACPYRKDFFAKLGDDLAKVNEQLQAWVSSLESVVKEIMDFFESGNYGKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKI8</accession>
    <name>A0A1E3NKI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_72708</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TOG/XMAP215 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017770.1">
        <property type="nucleotide sequence ID" value="XM_019164279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKI8"/>
    <dbReference type="GeneID" id="30180966"/>
    <dbReference type="OrthoDB" id="205662at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990498">
        <property type="term" value="C:mitotic spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099070">
        <property type="term" value="C:static microtubule bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048492">
        <property type="entry name" value="Stu2_CTS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21042">
        <property type="entry name" value="Stu2_CTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="9">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="9">
        <location>
            <begin position="271"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="6">
        <location>
            <begin position="440"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="522"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="611"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="540"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="781" mass="87146" checksum="46FF803CBECD06F7" modified="2017-01-18" version="1">MSESEFENLPVEELLEHKLWKARQLGYSQLKEIAEKSISDELITQLVNNPQRLLKIVTDSNVAAQEMGISALTTILKQSRPETVSRTRDTVVPGLVEKGLTSSKASSRSEATEALLLYVELDSGKEVIELMLPSLSAKSPKQVTATVKAINDIYSAFGCPTMDPKLIINSIPKLFEHSDKNVRGEASGLSVTIRSYIGDSFDQTIFPKIKPIQQKDLKALFEEIKSGAKPSRMTYSQKSANLAENDEDVQMTEESVIESTGEAKAVFNAYDFEDPVDVLSQLPTDLSTRLRESVWKERVEVLEEISGFFKAPKIQDEDYSYFISLMVGCLRDVNLQVVTLACGILLDLAKGLKANFHKYSSTLVSPLLERTKERKKTVLGALIEVLDTCFKYSSFHDIVDPTVEYMVHISPHVKVESMQYLVRCLKETKVLPTNDEIERMMASAIKLLQDSQVTVRNAASEVIGTLMKLIGSEKSRKYLDRVDKRHVKKVQQICQSAVVKTGPTREGFTVVEQVTSINDKIPNLAQRGVGSNTSENENTTAAPSTSRSSGSSIPSKRAATSPLKENVANHKNTLTSKPLKASNVSNYGISAQQLQELELLKAEKFVWLEEKKVLLSDLEEVKSNNDSLLKNVVSLNGKLDDYHEKFTTMSMTLKSKDTQIFRLRSDLETCQIRYSQLQQKNKVLSGQLELSNDKNTAVESINKVPEDSNDINRRISILSIGSSTGEAKETEKIPVLRNNVSIYNFDDADDGWKRATAVTNDLKAKIQRMKARTRVLDPVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKK4</accession>
    <name>A0A1E3NKK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_33768</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017780.1">
        <property type="nucleotide sequence ID" value="XM_019162843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKK4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKK4"/>
    <dbReference type="GeneID" id="30179530"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000003">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF4">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="23"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="474" mass="52540" checksum="4520B4B5F6E0E7CF" modified="2017-01-18" version="1">MSSPASVSHVWDTQYDAATILTLVTGTCLVLIMCFGLAYLYSGLARRKSALHMVFSVFLILLISIFQWYLWGYSLAFSATASNRFIGNLHNFGYMHLDQALVVDATHHPQGIPEMAYANFQGMFASITACIYIGALCERGRILPFIPFTFCWLTLVYCPVTCWIWNSSGWAYRWGVLDYAGGGPVEILSGFSGFVISYFLGPRKEHLMINFRPHNVSLVTIGTMLLWFGWLGFNGLTCVTPSLKSVYAIMNSNLCAAFGGFTWVLLDFYRTNHWSTVGLCSGIISGLVAATPTSGIIPLWASVVLGILSGCICNFATIFKVWFQVDDALDVLAEHGIAGVLGLFFNSIFAADYVIGYDGYTEHPGGWINHNWKQMYKQIAYIGAVVGYCCVVTALLCYVLDKIPYCKLRADEDAEEMGMDEDQIGEFAYDYVEVRRNFFDVNGFEGVVPESPAPQTRPVSNASDQEKTADDSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKM8</accession>
    <name>A0A1E3NKM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72740</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017807.1">
        <property type="nucleotide sequence ID" value="XM_019164283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKM8"/>
    <dbReference type="GeneID" id="30180970"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="189" mass="21769" checksum="8BC6F11669878E4A" modified="2017-01-18" version="1">MTSIKPFQATDLFNLTPINLDPLTENFYLYFYHQYLLTWPTLSYKSVNQSGEPTGYMLAKTEGSAKEWHAHISAVTVDPNYRRVGLGSYLCDQLKIVVEPERPVHAWFIDLFVKCSNKTAITLYEKLGYSVFRRVIGYYGSGNAPIDKKKVDDDVDAFDMRISLQRDIKNETVRKDGRKTHVLPEEVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKM9</accession>
    <name>A0A1E3NKM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73592</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017231.1">
        <property type="nucleotide sequence ID" value="XM_019164528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKM9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKM9"/>
    <dbReference type="GeneID" id="30181215"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000907">
        <property type="term" value="F:sulfonate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044273">
        <property type="term" value="P:sulfur compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="84"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="403" mass="46659" checksum="F3E561A3E7C05202" modified="2017-01-18" version="1">MSVLRRTYHDGAIHFQKDSDEKTDGILTVSKENLKRLKYPEWAPTWDPKDDHAFQNPKPFKHVDRGFAGDAAFKSLRDVEGVKFRQISPKLGLEVEGIQLSSLTDQQKNDIALLVEEKGVVAFKKQDFKNQSFEEIKNWGRYFGPLHVHPTSGAPLNHPEFHMTFRRGSKKEQEKFFADRLNNVSWHSDVTYENQPPGITLFAMLQSGECGGDTQFLDMFEIYDRLSPLMKEKLDGLKAVHTSKEQVSAAKQEGGIERKDVIDSIHPVVRYHPVLKRKSLFVHRGFTRKILGLKTEESDNLLLFLFNHTAACLDAHVRMQYEEDTVVVWDNRRTLHTRTMDWDSEDIRHAFRVTSIGERPVGSQEEFESWTPELEEANIKLTDHYINLSAEEYFNEVIAKERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKP9</accession>
    <name>A0A1E3NKP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17158</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017016.1">
        <property type="nucleotide sequence ID" value="XM_019161490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKP9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKP9"/>
    <dbReference type="GeneID" id="30178177"/>
    <dbReference type="OrthoDB" id="1792at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="3">
        <location>
            <begin position="532"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="861" mass="95567" checksum="65341F1EAAD8C144" modified="2017-01-18" version="1">MPVSNPSLNWELLDTVYYRSRTCYDSLDWPASASSSGPALHNVDLAAFNVAVGPFASLVALYPKSLSRPQAASEILVCSGAGQLLFSVPWNFVLNPIARLGWAPSGELLVVTAAGKYRVYYNYQGDFDEFDILTDLLQLDDTDSVTVLNALFTNTGFAVQSSNNHFAYIQNHRKPSARTAVLCSQLATTEPIEPYHVTTWTLTSPPEDSRNSLAKAKFYVFTSAGLCVLTPSTPVPSFTNKPLLTSVAHVDIAPNNSFAALYSSSSSTLYILDGDFNDLLAEHKLEKPPLQISWCSNDVVVMAYLDSISVVGPSSDTLEFYTNGTPLLKGELDGLYYLTNTELCFLSRVLQVTEDTFRIGSTSPSAILLDAIDYLDRHSPKANDHLEIIDDHMVMAVDGCIRAASEEFDLYWQKNLLRAAAFGKVNLDLYDPTEFVQTCDYLRVLNIVRSQEIGMFMTHAQLHSIGIEKIIDLLLLRKMHFLCLKISAFLNLPNYKILTDWACCKLKHSSGLDVDNDKLLASIYEKLKSSKIDWTHIAYVAYTEGQQKLARNLLSYEPNTSKKVQFLLDINSSATGDEIEYALTKADEDGDVDSLVLILLELYGSLSSAEFFRAIDQKPSAIGVLKSVFCQIDEKGGMLKNFMFQDDDIIGRLIIDIVSAVKSNKPIESTDLTKLQSLAGRSKQTQSAVPYIKSDIKLLKLQVELQANLHSILPGEPLLDTLQKIIIVDLKQAITVARKFNIPPKQLTGLVLKVLAAKSDKHTELYDYATINGGGKIIGFETFFYELAKLGERRQAGLYLPFCKKMTIKQKIRSYIFCGMWKEAVAEAGSKNEVGLLKSMAESRTGWEASVASDEFERLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLE3</accession>
    <name>A0A1E3NLE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_59133</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CybS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018054.1">
        <property type="nucleotide sequence ID" value="XM_019163345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLE3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLE3"/>
    <dbReference type="GeneID" id="30180032"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="157" mass="17263" checksum="537E7FB8FB2C5124" modified="2017-01-18" version="1">MLAFTSRIGLGFSRKSILQSTIAYRSFFKTIPQPPGNIIGTVNDAYVPPKPNKLHGSLHWTFERVVVLGMTPLIVAPLVTGSSPFDGAMCALLLYHCYAGFQSCIIDYIPKRAFGVFHSLAMGLLTFGTLLSGYGVYKIENEEQDGVFGILKKLWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLN4</accession>
    <name>A0A1E3NLN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71179</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018168.1">
        <property type="nucleotide sequence ID" value="XM_019163852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLN4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLN4"/>
    <dbReference type="GeneID" id="30180539"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="7">
        <location>
            <begin position="106"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="798"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="799"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="824"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="850" mass="96858" checksum="5680C1107E2ABA26" modified="2017-01-18" version="1">MNDYQIARNLGEMFENATRSLNLVFDTKNIALPPSVTSFINTNYYNVETTFTTKEIIKGLESSNDNEVYTVLKHLVGKLATRRDVSEVEGGNAVGYGFDENSETVKEIVQLFPHIIKNVNSANLKIKRLVYIMLLSFNHLQQDISLLSINAIQKSLTDKSAINRSLAIRCLSGIRIPAILPILLLSMGKLVKDSSPLVRAACTIAIIKCAELDTQYNMIKRTKAKTKKDFKSKHAKNTESIVGANGAKLQREYMVSCLRDETSTIYQLSSYLDILLSDNDPKVLSCALVTYHELFNGCFDLLHNKVNNLVNHIEDMDSFATSNLIDIMTDYSRLFFEPFTRLEDAPRELRDLYGHLMNMVYYSMDYNVTFSIVKCLVTLYPFTLSSKDVRLDKIMIKLIQKDSYDDINGDARVSISLNMILYLLNKELVSFRSYQFSNFIPSTSDNPAIFDTKISILFKLISEENFEVVFKEMKFLVEKSDFNYFFKFKILEQINSLILSKNLHVQQFSKLIRFFMKKLQYEKNELLVGEYITGLRQLIQFNLGYYNEILIKLVGRLVESYNDDNDVKLLHNAKASIIWLLGEFAIDFSDYHASENITVLKNALPDLSLLLVEHFKEETEFNVRLETLIFVTKLMISDIVENGKANYRIQNNKLFKVFNYVLQLAKYDIELYVRDMSRLIGSLLPNVVYYSNLLVTQEVNIDEMLNSDPVLLSHCLDKCSSVDLSVLMFQLSKPQIRLGGVDESDKDEDSLMASVAKFSQVGTGKLDPGYLSYYRDLRTEGFELRDYGKYSSSLSSASYKNHSQTQHAQSPVADSFADSRDRSLSSVSSGTSSGAGKYKLQTLDDFLGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLU4</accession>
    <name>A0A1E3NLU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SURP motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018218.1">
        <property type="nucleotide sequence ID" value="XM_019160669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLU4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLU4"/>
    <dbReference type="GeneID" id="30177356"/>
    <dbReference type="OrthoDB" id="447637at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000002">
        <property type="entry name" value="Splicing factor 3A subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045146">
        <property type="entry name" value="SF3A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022030">
        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="18"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="131"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="304"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="460" mass="53614" checksum="6D833E5EBC4DEB09" modified="2017-01-18" version="1">MSAIPSDITIPPPEIRTLIGKTAIYVDKNGKAFENKIKTKESKNPKFIFLNEKDPYHAFYQYNLTVIKSTGKPPVINQGNEHIIQKEIATGTANENKKEDLSEPESYRFLEFKDHKLIYPEEKISSLDLNVIKLVAQFAVVNGSKIMNDFKENALNNSRLSSQFQFLNERHSMNKIFQKYVDIYNVIWTQKRELLDQYGKDTNIDDILSNCFSRAEYLERESTNVQEYEERKLLERMTYGSIDWQDFDIVETIEFTDLDEVAELNVPLVKSDLEYRSLIQKGNSNIFAQIEESTRMRDQLEDYEDEKVDEGDEDDAKAQDEGSVPQYEDEKESEEEEEPAVEKSEPSEEAKPTKRVPKGIKVRSAGESRLLKRRFQSQSEENMIDPVTKEKLLRCPLTDELIPESKFQGHISKLLRDPKYEEEKARYESKFKYGSNLSTEQVYENIQRLVEDKSKRPKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM69</accession>
    <name>A0A1E3NM69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_52477</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017791.1">
        <property type="nucleotide sequence ID" value="XM_019163164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM69"/>
    <dbReference type="STRING" id="763406.A0A1E3NM69"/>
    <dbReference type="GeneID" id="30179851"/>
    <dbReference type="OrthoDB" id="10027823at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF2">
        <property type="entry name" value="ABR173CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="3">
        <location>
            <begin position="14"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="441" mass="49087" checksum="7CDA2D0C6C774ED7" modified="2017-01-18" version="1">MTHIFLSLKQSSRDIRLLWFSVFIRMISFGLTNQVLTLYLKEIGISEVNIGLFMSLTMIGDSVISYALTWNANKIGNRFIMLMGSFLMMVASGIFSTGTSNFGYLLVAAIVGVISPSGNDAGPFKTIEETVLANLTPPNHRPEVYAIHWTLGSIGASLGSFLAGLSVDYFTHKRLLPVVDSYRYTFCILVFTSFLKFVSLLFVSYKAEPSYVPPYMAVQHDQDSNIEDATIEHETLTGLSPQSQSILFKLMVPFMLDSFGNGFMTNAWVVYYFKERYKTPAVLLGTLFGLSGIAMSVSAIPSAWFAKQFGPIKSSVMTQIPCGLFFMMIPILGFNFSLGSLFYLLNQMTTAFDVVPRQIILTTLVEPTDLSKVMGSVNIGKQIARSISPYFTGYFAEAQLLWVCFLITGVLLIIANLILAIDFSDLDEKVREIEQVQHDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM90</accession>
    <name>A0A1E3NM90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_31237</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018344.1">
        <property type="nucleotide sequence ID" value="XM_019162684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM90"/>
    <dbReference type="STRING" id="763406.A0A1E3NM90"/>
    <dbReference type="GeneID" id="30179371"/>
    <dbReference type="OrthoDB" id="391817at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF4">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="7">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="370" mass="42481" checksum="1F50867A87148C25" modified="2017-01-18" version="1" fragment="single">MRVDGAIPPLPSNDCIRLVSFNINGYRTLNHYHPWNELPALSDIFNYLNADVVTFQELKLQRQDITRSLVSGKGFHYFITLPKTKRGYSGVGVFVKNSRQDLQVLRAEEGITGFLEVSTDSTLNYRKIWERDGPSGLAIGGYTDNIADWKEGYQLDSDGRCVIVELNNNIVIFSVYCPANSMGEEEGEVRRCLFLDVLFKRAENLQKMGKRVVIMGDVNVAPSLIDRDDVMNTGFRDKTLFRQKDPAGSFEKENKKMTLQFRSETLPRMILNDYLYDYNEFDKIKNTKKILYDLGRLQNLNRLKMYTCWNTLKNTRPLNIGSRIDLFLATKDVKDIVEACDIWSFLYGSDHCPIFCDLNIGKLPPSNTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM95</accession>
    <name>A0A1E3NM95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inclusion body clearance protein IML2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15231</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IML2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018363.1">
        <property type="nucleotide sequence ID" value="XM_019160729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM95"/>
    <dbReference type="GeneID" id="30177416"/>
    <dbReference type="OrthoDB" id="2154985at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="632"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="89362" checksum="FD1DDBCCEF87F5D9" modified="2017-01-18" version="1">MLRALGFKGSASLTQEEINQKVANQALQLERALKAMDYVLDDRAEEAFRLLDDHDNIAISQLAYGVVQFLEATLGFEPETMKKASETLSKAETMAWKEKTSAERNNVKSSQIYPPGTEFAVAYAESNLLNALLMLLSENFMEGAKALLKLRRAYQTLDAISKHLSTPPDASSSALADLDSYPTCSKSNSSSFVDLPLPLTEQELKDRKIIDELDQTYHMRKSRINGSHIGNPPVSDSLRSNLGFKDESATPASDKDVLKFGNPEVASVDEYIHSGVNLCFGILQVVLSLIPHGIGKVLSVVGFKGSREAGLQMVWNAAQERNIHGGIGLLALLVFYDGPFQFTDVDFDVPTAEELSQNDDKTNKDIDTEMEKETAQTLQKIKTSASTASKKELVEDKIALERMATSGHGETLLHPGEKLVKVLLHSRALFPHSNLWLLQESRMLASRGRLHEAVDLMDTAKPSQMKQVDALLGFDRCMILVFLHHFDRAASDFLDLIKINSYSHALYTWFAAACYLEAYRMSLTGLIPKDTDVDPSKIDTYAKLAEKYIHEAPKLIGKKKFLSKIPPFEKFIARKYKEIEDSHNSHPKTPFIDCIQTSLVHELAYFWNGYNRMSTECLQLSISLLAYSATPTSLSSSSRTSSDVTSGNSSMSIHSSASNKTLDLLPSPIALTLTNKETGLPYAKIHESKEQRIIRVTLQCLALRRLGYIKEGLQIFDKVVISNLILPDGRLTKLNENPYLYPTALYERALFTWKLDGADGLAECMKWLKYSQAYGGDDYELSTRVTMKTKAAIDRLEDLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NME3</accession>
    <name>A0A1E3NME3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor MBP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_30738</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018339.1">
        <property type="nucleotide sequence ID" value="XM_019162653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NME3"/>
    <dbReference type="STRING" id="763406.A0A1E3NME3"/>
    <dbReference type="GeneID" id="30179340"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000004">
        <property type="entry name" value="Transcription factor MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="102" mass="11666" checksum="C49966F3E3D68F87" modified="2017-01-18" version="1" fragment="single">MHENLYSATYSGVAVYEFIHPKSSVMRRKKDGWVNATHILKVANFPKAKRTRILEKDVQTGVHEKVQGGYGKYQGTWVPLKRAVEIAQQFGVLNDLNPVFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NML7</accession>
    <name>A0A1E3NML7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetyl-CoA C-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15318</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018472.1">
        <property type="nucleotide sequence ID" value="XM_019160765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NML7"/>
    <dbReference type="STRING" id="763406.A0A1E3NML7"/>
    <dbReference type="GeneID" id="30177452"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000007">
        <property type="entry name" value="acetyl-CoA acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF165">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="9">
        <location>
            <begin position="274"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="397" mass="41212" checksum="9EAC0AA738E0EB13" modified="2017-01-18" version="1">MSKEYVYIVAGARTPIGSFQGSLSSLGYVDLGAHAVKAALEKVPEIKPTDVEEIFFGNVLQANVGQAPARQVAIKAGLGEDTPATTVNKVCASAMKAVILGAQTILTGCADIVVAGGAESMTNCPYYLPNARGGLKFGDSTVVDGISRDGLNDAYDGKAMGVAAEKCASDHKISREEQDDFAIASYKKSQKAHDEGKFKNEIAPITVKGTRGKPDVIVERDEESSRLNEGKLRSARTVFKKENGTVTAPNASPINDGGAALVLVSERKVKELGLKPIARILGWGDAAHAPVDFTTAPSLAVPKAVKHAGLTLDDIDYFEFNEAFSVVGLANAKLLNLDLAKVNVYGGAVAIGHPLGCSGARIIVTLMSVLAQESGKIGAAGICNGGGGASAIVIEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMU8</accession>
    <name>A0A1E3NMU8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_34061</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017826.1">
        <property type="nucleotide sequence ID" value="XM_019162863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMU8"/>
    <dbReference type="STRING" id="763406.A0A1E3NMU8"/>
    <dbReference type="GeneID" id="30179550"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035841">
        <property type="term" value="C:new growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035840">
        <property type="term" value="C:old growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903139">
        <property type="term" value="P:positive regulation of cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070610">
        <property type="term" value="P:regulation of fungal-type cell wall (1-&gt;3)-alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000573">
        <property type="entry name" value="RHO family GTPase Rho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="199" mass="22036" checksum="6BA1340ECB68D575" modified="2017-01-18" version="1">MSTNPNENVIRRKIVIVGDGACGKTSLLYVFTLGEFPTEYHPTVFENYATDCRVDGKPVRLTLWDTAGQEEYERLRPLSYSKANIILIGFALNAPDSLENAAVKWAQEVKQYCPNAPIILVGLKKDLRPADDGSDPHYSSRYVKTQDGDRVAHYIGAKKYIECSSLTGEAVDDVFEIATRASLIARPLERQSSGCCTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMX4</accession>
    <name>A0A1E3NMX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018588.1">
        <property type="nucleotide sequence ID" value="XM_019160821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMX4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMX4"/>
    <dbReference type="GeneID" id="30177508"/>
    <dbReference type="OrthoDB" id="447103at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="721" mass="80602" checksum="DF008205545ABD85" modified="2017-01-18" version="1">MNFLTKTLGSLTGTSIPYTFGSIVQPSHLDSPSSTSLWKIHDATSDKDSRPVTIFEFDLKHPGNSRYVPLVQNAVKKHRALALLPGVLSIVEIIESEKTIYLITEHTIPLSHIISEYSGELKLLGVFQLTLALKFINIEGSCVHGNPRLENVFVTDSREWKLGGFEFTINYKDDSNDYLTLYSMYTSLTSSKGLIVPPEFESSGSDFFKGLGKGIKGTKFDSYLFGLTAYQIMTNKPPSVAEIMRSGSIKGLTLNKLVAPSIGLRITIEQFMSNGEMSYFATKEIHAYSKFSQISLLSLDQKLEVYKALVSGNTPTEFLEIKVMPEIANTFDTITSNENNVQTTLIYLIYLIHSECEGESKYFEMFFKPLYFKSFLLADRAVRTIILKILPKVIDKISKHEVQDRIYPNLVSGFADTDITVRTETLLSISYIMDKITDRQLNNDLLRYLAKLQADQNPQLRANTVICLTRISAKMQSTTRIGVLVTAFGKALRDPDYVTRLCAVRGFESSIDYFTPEICCSKVLSALSPALLDTSSIIRNEAEKVFELYMKKIRDASTKLHKIEEDTHILDDVSNLTNLLNSLSLENLGHNLLDSISNLHTSTPYPESRMGGNSLNLANKLTSKDNLIDEDFDLDDDVDDWGFDDGESESKSGTENGLSSTWKPSETSKPSNSSEKKFTFPSAKVNEFKKTSLVLGKKKTAAKLNLTVEPVDDDDGWGDGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ0</accession>
    <name>A0A1E3NMZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_19657</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018597.1">
        <property type="nucleotide sequence ID" value="XM_019162133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NMZ0"/>
    <dbReference type="GeneID" id="30178820"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF28">
        <property type="entry name" value="REGULATORY PROTEIN ADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1186" mass="135202" checksum="1BCD14337643A541" modified="2017-01-18" version="1" fragment="single">VSQEFTLKGSTPSGKPRLFVCATCTRAFARQEHLKRHERSHTKEKPFTCNICSRKFSRRDLLLRHSTKLHAGAADVIPRLRKRSMKHMKKEELEQLQREQDADIDNHHDPNNKRTKANSLSTATASTLISAASVLTGITSNASNASSNAVNNNPIHLNDSDSNFDRFNELQSNAYNYYRRASFSAMSGVNYALTNPEPYASETVEFSTPQFNSLEDEQEKWLNSFSDNNDINGIEKDQSIPSSLTEIEQLNNFINSRIQKREQQQNDRLVKFSDQPQQPIQSGTHHQQLKQTSAIRHSILNATDGNYPKNANDTEKLNLNSDFFDNFLDDNDSGVLEGVTKKVLSWQETLFNQPITDLEKLNEMHIGKAYGVPQGYSFYGGDEAGPMNRSNSNGLLGTYNDGSLSSDATISPVLLEPSCSVGHIEGDDEVNKHNKIPRTHHSHHRHLTFEEEFSEATLSSCSQAFLFTNNIARLVNKGLSDYPFFGTPTPELLQNDVLNLYVDQFIGKFLTHHPFIHRGVLNEYWLMKEAIDAAQENSISVTESESYQGTSLKLVRDENFVENFKVSIICLPLLITTIGAVVSNKREDAAILYEISRRCIHVYLESRKKLRTCGGVGGIGLSASVTESSPLWLIQSLTLSVIYGLFADEEISLSVIIRQVHALNALIKSSGINQLRVPDKINSDYKSFINHESTIRTIHMIFHVSTLLSTLYNIVPSLNISDLNTDLPSASLLWECSSAEEYSKLIENFDFHAKNFKEVIRELVKLDLDQVDPNTGLVDNRNFLLDYHVSEFGLICLQNGLHQLVYFKQLSNEDDPNDKQVNDNVANLGKCILADVGEGADITIEKIRKIGEVWNLLVKACVLYNKSSEVYNDCKILNHYLNLKLSSIVSLNKIKESVWLKSFSDINEQYIACFNYGDDELKDQHFQTELIHLIENSINILKLLASSSSSDISPEDKEELANSYNYNSLNKINLNVLHKLSIDSQILFDVILIIVKFLTNYENFFKIRMKFNNLTNYTFLQHFEMSSAIFLSERGEGAGDDNDGVEEALQRFDKVLFKYYLKFFKIFLNLEQFLKHNYNYEDFETGQAGDFAGVEIDRVLRESHNKFREEERTLNDIVSDELVVFHDRDLIMSELVNFKLPFKFLKMGGFLFGFIYDKNFKFVNFKHLSDVLFHLRIFLESKDEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN24</accession>
    <name>A0A1E3NN24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_67133</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018618.1">
        <property type="nucleotide sequence ID" value="XM_019163498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN24"/>
    <dbReference type="STRING" id="763406.A0A1E3NN24"/>
    <dbReference type="GeneID" id="30180185"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF15">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="451"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="8">
        <location>
            <begin position="81"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="587" mass="65336" checksum="F1FD32DBA474BAF9" modified="2017-01-18" version="1">MGFFNRSEKTPDYADTSIEVLHDDHGASSSSANEKKLYEAKVYDSASNTNGSSGPFDDHDSDDSSAIDVNAHTERGLKQRHIDLIAIGGSIGTALFVTIGNALMNGGCGNLLVAFAIHGLLVIYIVTLTIGELICYLPVDSPFITHAGRFVDPAFEVMCGYNFYIMVSLYIPFEITSVNSMIHFWRDDYNPADSFVPQIVIYLVLNVFAVNFYGESEFVLAIGKLILAIGLLFFTFITMVGGNPQHWAFGFHNYKGVNNAFPEYLGSGASAFWAAYLKAVFTEVAPEYLGMVAGEAQNPRKTMPRAFKGIIYRLSIFYVLGALSVGILLRHDDPTLVAAISNGAAGANVSPYVIAMQNLKIKVLPHIVNVLCMTSSFSAGNSYIYCSSRALYAMARRGKAPKFFTICLKNGVPIFGIAMAVAWSCLSLLQLGDTASVALDWMVNLCTGCQVLNYFFMMITYLCFYRACKAQNVDRLTMPLQAWTTKLQPYPAIFALVTTFLIVMFLGAQAFLPSFDVNSFLYYYLMVFVDIGVFVLYKLVFRTKRIKPEEADLFSDLAQIDEHERKFYAKHEEEKKEGWTKFMNWIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN25</accession>
    <name>A0A1E3NN25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7907 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_27248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018630.1">
        <property type="nucleotide sequence ID" value="XM_019162490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN25"/>
    <dbReference type="GeneID" id="30179177"/>
    <dbReference type="OrthoDB" id="4018368at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057229">
        <property type="entry name" value="DUF7907"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25484">
        <property type="entry name" value="DUF7907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7907" evidence="1">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="109" mass="12060" checksum="981D819C325BE4F9" modified="2017-01-18" version="1" fragment="single">DEFGKTGITNYHEGAGINYLFLTGSDSPVYTYNSCSSQIYVPFEENYKQFFNANSKGGIVQLTVGGPGGKIDVNEDEYLTLDGDAHGWVACKNTGDPYNYSRDLYQLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NND1</accession>
    <name>A0A1E3NND1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingoid long-chain base transporter RSB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15146</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent translocation of sphingoid long-chain bases (LCBs) from the cytoplasmic site toward the extracytoplasmic side of the membrane (flip-flop). Involved in the establishment of the functional lipid asymmetry of the plasma membrane. Regulates intracellular levels of LCBs, sphingolipid precursors that are growth inhibitory at increased levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018271.1">
        <property type="nucleotide sequence ID" value="XM_019160685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NND1"/>
    <dbReference type="STRING" id="763406.A0A1E3NND1"/>
    <dbReference type="GeneID" id="30177372"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF9">
        <property type="entry name" value="SPHINGOID LONG-CHAIN BASE TRANSPORTER RSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="35940" checksum="000DC897F49C1BFB" modified="2017-01-18" version="1">MDSSAALAAVGAASAGAELYARSFIQGPTPKLASGIGYVVVFSVLFAVNMLSCVFYHQWWFLGSWGVGLVLEIVGYAGRIWYTLNDNSSSAYIMELVCITVAPCFLMAGIYYVLAQLILIFGNDFSRLKPMQYSLIFIICDVISIFVQAAGGGVSDNPSSSTIGRYIMIAGLAFQVFTISVFQVLWYDFLWKIYNTRKLHGDTLFNPKFPHIRARPLLTPFIWGVSLVVILIYTRSIYRLIETSSGWESYLSTNEIYFNILEGVMVSVSALIMASLSPGFVYGRHAHLYIKKDSYSSATAESDGRDEADETLIEAETKGSRDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNR3</accession>
    <name>A0A1E3NNR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_10701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018853.1">
        <property type="nucleotide sequence ID" value="XM_019159708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNR3"/>
    <dbReference type="GeneID" id="30176395"/>
    <dbReference type="OrthoDB" id="3997999at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="505" mass="56407" checksum="3B4215D78FDA5DF6" modified="2017-01-18" version="1">MSADGLWIAHYAPPTETLTVHRLPAKQSAQSHVYSIESLSLRISPVSSLCMHPEFPVLLGVPLEKTGSSIPRGPSSLGSSAPNSLKPLSPSKTSDTALTTTKTKTKIQLTLFSFYSRPMVFTPLIVEVEAPADRTLSPWCIKYSPSGKYLAVHARSFSNSGLDTINQIYIFDTKNNYSLVTSTDVESGIEDLDWNHKSDTLIAAGSHGQLHLYEFDQMNDELLLKKSMKLSSSALSSICYYEEINEKDDISAGLLVGTRDGNTLFIDPSTMCCTNSVLFDVESPVLKVSLGPNGIGIVSYLSCNNEPAYIIRISQQGDEYEQIAEIDDIFPTYNAASDNANATTGCGALVTKFGIMVYVKKDGKVCFETLSELLSKKDKTKAKVETVTSKATNPKKRSADLFNKIKDKENLGTSSGVSLKKQKDSYGKSQREYWDSRQGVREREKVGREKDVADRGRDRGRDRGRERDRDREREIDRDRARERGGRESDRDRERDSWRYKRVNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNS0</accession>
    <name>A0A1E3NNS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber evidence="5">2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">GlcNAc-1-P transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_71782</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13290"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.15" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018863.1">
        <property type="nucleotide sequence ID" value="XM_019164004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNS0"/>
    <dbReference type="STRING" id="763406.A0A1E3NNS0"/>
    <dbReference type="GeneID" id="30180691"/>
    <dbReference type="OrthoDB" id="10262326at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06855">
        <property type="entry name" value="GT_GPT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033895">
        <property type="entry name" value="GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571:SF0">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133462" description="UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase" evidence="20">
        <location>
            <begin position="32"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ47750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="462" mass="51882" checksum="AEAAF99EF5E81488" modified="2017-01-18" version="1">MNKTTSAASLLISAVLVGLQTPLQPIQSALGFSIFGYTCVDYLIPKVGAVFIKRGIFGKDMSKVGRPIIPETIGVIPALVYLLVMFFYIPFLFARKGNNGGGGEADASAFSHSKLSSYLSSLLSLESMILLGLMDDLFDIRWRHKFFLPAIASIPLLIVYHFEFNQTSILLPKFISNAFQLSTSVDLGVLYYFYMAAVSIFCPNSINILAGINGLEVGQTLVIAVLLVINDLYYIVGYFLEDHLIQSAFEVHMFSLCFLIPFLGISLALYKYNKFPAKVFVGDTWCYFSGMVFAVIGISGHFAKTLMLFFLPQILNFVYSSPQLFGIVPCPRHRLPKFNESDGLLYNSYTEYEDSEESAESSDGKKKQLRPYLKPVFNLLEKMYLIKLVRNEKGEIVKSSNLTLINLVIIWFGPRNEGKLCTLIMQLQFAVGLLMLILRHTLAPTVFGFDNSWSMLNRFYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNY2</accession>
    <name>A0A1E3NNY2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_10385</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWI5/SAE3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018501.1">
        <property type="nucleotide sequence ID" value="XM_019159662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNY2"/>
    <dbReference type="GeneID" id="30176349"/>
    <dbReference type="OrthoDB" id="255837at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032798">
        <property type="term" value="C:Swi5-Sfr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034974">
        <property type="term" value="C:Swi5-Swi2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010772">
        <property type="term" value="P:meiotic DNA recombinase assembly involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010760">
        <property type="entry name" value="DNA-repair_Swi5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07061">
        <property type="entry name" value="Swi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="28"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="136" mass="15667" checksum="97D5E3FD8BD09938" modified="2017-01-18" version="1">MEDKENAGENISSADADPEIDVAKSRKEIFVEAQGNKLEDKIEKLEHELIQLKEEHLKLSNELSREKGYVKGVTDGSFNQSFQKDYDKEMKRHIRQLKKYNELKDLGMGIIQLIADQKQSTLRGIMDEMGIEPDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP35</accession>
    <name>A0A1E3NP35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_19863</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018967.1">
        <property type="nucleotide sequence ID" value="XM_019162143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP35"/>
    <dbReference type="STRING" id="763406.A0A1E3NP35"/>
    <dbReference type="GeneID" id="30178830"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902953">
        <property type="term" value="P:positive regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070863">
        <property type="term" value="P:positive regulation of protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009917">
        <property type="entry name" value="SRA1/Sec31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07304">
        <property type="entry name" value="SRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="118"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="SRA1/Sec31" evidence="17">
        <location>
            <begin position="1079"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="763"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="803"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="853"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="918"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1000"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="773"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="870"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="891"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="965"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1043"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1094"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ47854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1217" mass="131575" checksum="F2252C8F5B33F337" modified="2017-01-18" version="1" fragment="single">MVKLHEIDETAIFAWSNDALPLIATGTLSGVMDDTFSSDSSLSIYDPFATSGTKQASAVYTAAAPGKFASIDWSKAAEGHVRGVLACGLENSSIQLYDPATILSQKPANLVDARIAEYTKHTTPVLQVKFNPLQSHILASSGSKGEIFIWDVKKGTSFNPGQAISPMSKVSSLAWNNNMAHIFGTAGDNGYTSIWDLKAKREVLQLNYSNINLSVIKWHPSQSTKLVTASDSDGEPVILTWDLRNSSVPEKVLKGHKKGILSLDWCLSDPNLLLSSGKDDSTLLWNPIEGVQLATYPSLSNWVHETKFAPKIPEVFASASLAKKICIQSLQDTSEPISAKVQSKDENDFWNEISTTETQQPLFKVQQAPIWLKRPISATFGYGGKLVISNKKEVKIVTVSNNGSIDQSAKEMITAISTKDFSNLCDSKISSGANLNLKESSDWSLLKHVLTDESLESLLKIEPSSPTETSRSDSTELIDDDDFFAQIGDSKKKSDSSAAAASYTPEGKFDLFSESSDQFEKDAVSLLLSNKTDELLDLCIEKDHILEALIIALNGSEESKDKAKSAYFKKYAKDSSFARLLYSSSENSIGDIVKNANISSWTQIAKSIITFSKGKASFNVEMKLLGDRLLDSNLENSRDSALSCYIAAGALDKVSKVWFSELRDYENFFLATKNDDGSKNTAFEARFKALGEVIEKIVVFQSKASSSLSGDLSELGKAFVEYADSLVNFGHYELAYKLLSLVSDSIPEIRFEKDRISKAFISNQQQQPLQQPESITSFTPNDSAARPKRAKNPYVLPEAISAPVQSSNPYQPNNIYSGVPPSSTPLDQNVNLAAPYQPGNGVSTVPMNGISNNTARANPYAPSTALDSLKNPQSFATSNNNVPDYATPPPFKKENVVPPSLRKDVGGWNDLPTHLAPVIKPTATPPNANAYRPAVSRSVSQAPAIPPPPARQSSGPVDSPPAANLVSSRMSSFSKNPYAPKPSVAEPVISPMYPKSPFAPQQPPVLGASSAPVANQSKNPYAPKPSVNNAYVPENAYSPQPPLQQSFQNQQLPNTNFNAKTMPPPPAYAKPVSTTVATPPPPPPKKLNSPGNAPLPAATPATSATPSAQLPPEAQPIVSILSAELEKVRPNIPAKFSKHLVDAEKRLNILFDHLKKGDLITPPTVEKLVSLSTALNEGQYAKAAALRDEILDAHSNECGDWMVGVNRLIGMVKATSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPA0</accession>
    <name>A0A1E3NPA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_31730</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019053.1">
        <property type="nucleotide sequence ID" value="XM_019162715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPA0"/>
    <dbReference type="GeneID" id="30179402"/>
    <dbReference type="OrthoDB" id="5414888at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013934">
        <property type="entry name" value="Utp13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08625">
        <property type="entry name" value="Utp13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="7">
        <location>
            <begin position="24"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="135"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="366"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="468"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="510"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="552"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="594"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 13 C-terminal" evidence="6">
        <location>
            <begin position="647"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="792" mass="88678" checksum="2D35A826EB00DE65" modified="2017-01-18" version="1">MSERTYKKINIEPFYVGSANATFSGDGSLMATAVSEDVVVINRLTNQIVHSIQGDGTDVTTVRLSPDGQKLAIVSLSQQLKLFDINKGEFYKSYKFSAAAYISACDRTSSLFAFGLTDGSVVVWDIEGSFITHNFKGHGSTISALSFHGDFNSKDWKVSSGDIMGTVKVWDLVKRKCIFTSKEHSAAVRGVNFDKSGELFVSGGRDNLLMLYNAKTWKEIKSIPLETTIESAGFVKINGANYIYTGGEGCVLYIWDFKSESLFAKTEKPLETTEELTITEVIQLCDVSDKLVMVFSDQTILDIFLSDDLDGTNHILPCTRVMAGNHGTIADLRYVGPSENLLALATNSPSLRVVDIYNKPMDMDICEGHTDLLNMLDSTVDGLWLATASKDNTARLWKYDEESESFTCYAVFEGHASSVTAIGLPRTPITDYPKFLITASEDLTIKKWSVPKPSGSFPIFVKSSDYTRKAHEKMIHAIDISPNNDFLATASHDKTSKIWDLQGGETLHILRGHKRAVYDIAFCHHDRLIVTASGDQTARVWNLEDGSCVRSYEGSSNAVQRVAFLSKNQYIVGAGADGLIKIWEVFTGECVNTLDNHDNRIWALRVKDNGEEFISADSDGAITIWQDCTDEANAEKELAKKVEVEKDQELRNCIREGDWVQAFMLALDLNHPMRLYNVVKECIAKGEDTESTIGSFELEELIGELEDDKILLLFKRIRDWNTNARLFEVAQKVIRVILDRCDLDKLYQIKGIMQYIDAIIPYSERHYSRFDGLVEQSYILDYVSRKMDELST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPU2</accession>
    <name>A0A1E3NPU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15958</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019247.1">
        <property type="nucleotide sequence ID" value="XM_019161034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPU2"/>
    <dbReference type="GeneID" id="30177721"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000168">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000034">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="238"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="461" mass="51764" checksum="728BF83C631283F3" modified="2017-01-18" version="1">MSEENDPLLAALNEDDNNQNQDQNPNSNGSGNGEEDSNEGQQTQTQSQAVRDADGDVEITNAEPEQREVIDPAEREREEALTALKNKLIEHLHYETKLKEMRKSLIDLDREFDKTEDDIKALQSIGQIIGEVLKQLDNERYIVKASSGPRYIVGCRLTLNREKLVKGVRVALDMTTLTIMRILPREVDPSVYNMTTFEPGEISFTGIGGLTEQVRELREVIELPLKNPELFQRVGIKQPKGVLLYGPPGTGKTLLAKAAAATIGANFIFSPASAIVDKYIGESARLVREMFSYAREHEPCIIFMDEVDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDSLGQTKVIMATNRPDTLDPALLRPGRLDRKIEIPLPNESGRLEIFKIHISSVQKQGEIDFEPLVKMSDGFNGADIRNVITEAGFFAIREDRDYIIQSDLLKAVRKMVDNKKLEGKLDYEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ78</accession>
    <name>A0A1E3NQ78_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019363.1">
        <property type="nucleotide sequence ID" value="XM_019161074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ78"/>
    <dbReference type="GeneID" id="30177761"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="140" mass="16212" checksum="CBD8632C0076D0CA" modified="2017-01-18" version="1">MSGNYNRRGPARGAPRGPYSQRPSNGFRGGRGRYQGNYANDKPYNMPNRNQYNNQYSGNFNEQYNYHRSNQNSPQYSYQRGARGQQPPYSRNNISTGQYTQTYNPQGPNNTNGHNVSYDSHQHLPPQIYQNQTRILLNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ97</accession>
    <name>A0A1E3NQ97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72252</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019397.1">
        <property type="nucleotide sequence ID" value="XM_019164134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ97"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ97"/>
    <dbReference type="GeneID" id="30180821"/>
    <dbReference type="OrthoDB" id="1618453at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000002">
        <property type="entry name" value="60S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="7">
        <location>
            <begin position="235"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="246"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="294" mass="33246" checksum="67FCF4398E6C1A4E" modified="2017-01-18" version="1">MGFKFTRSSAYLSRYQTPFRRRAEGKTDYYARKRLITQAKNKYASPKYRLVVRFTNKDIICQIVSSTLTGDIVLTAAYSHELKNYGITHGLTNWAAAYATGLLVARRALKKLGLDETYTGVEEVEGEFELTEAVEDGPRPFKVFLDVGLQRTTTGAKVFGALKGASDGGLYVPHSPSRFPGWDIEGEELDAELLRKYIFGGHVAEYMEELMDDDEERYSLLFKSYIADDIDADAVEDIYLNAHEQIREDPSPKKSADKKAEYAAASKKFQQKKLTRDERKARVEAKILEFKASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQC1</accession>
    <name>A0A1E3NQC1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_79905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019420.1">
        <property type="nucleotide sequence ID" value="XM_019164976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQC1"/>
    <dbReference type="STRING" id="763406.A0A1E3NQC1"/>
    <dbReference type="GeneID" id="30181663"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF8">
        <property type="entry name" value="BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="274" mass="29668" checksum="7C9AA17CF68C4677" modified="2017-01-18" version="1">MSSSPVKVIVITGASRGMGKSMAEILLKSSDNCKLVLVARSGDKLQEFVDSLAEEDKKRVHFVAGDLTDSRTLDKLVDESVAKFGKINSVIFNAGILEPIAHIEKIDISQMKKLFDVNFFSLVELTQKLLPILRITAKQDGVPATCVYVSSSASEVRYDGWLAYGASKAAVNHLAMDLDAEEDGLIRGISIDPGVVDTGMQVNIRENLGRGMKSDAHKKFIEFHEKGQLLKPEVPGGVCALLALKGVPSDLGGKYLEHCDKSLSSFQFVGKNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQG2</accession>
    <name>A0A1E3NQG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_21994</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019061.1">
        <property type="nucleotide sequence ID" value="XM_019162231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQG2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQG2"/>
    <dbReference type="GeneID" id="30178918"/>
    <dbReference type="OrthoDB" id="1594986at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120171">
        <property type="term" value="C:Cdc24p-Far1p-Gbetagamma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120157">
        <property type="term" value="C:PAR polarity complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043577">
        <property type="term" value="P:chemotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072697">
        <property type="term" value="P:protein localization to cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13246">
        <property type="entry name" value="PH_Scd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010481">
        <property type="entry name" value="Cdc24/Scd1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033511">
        <property type="entry name" value="Cdc24/Scd1_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053026">
        <property type="entry name" value="CDC42_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339:SF1">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06395">
        <property type="entry name" value="CDC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15411">
        <property type="entry name" value="PH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="108"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="242"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="763" mass="86169" checksum="EB482C920B718FB4" modified="2017-01-18" version="1" fragment="single">LTNRQGSSDESLYYICLRLMNSLSKVPGMLPYIELAYAEADSCAEQQALAQSSLAASPSSPQRPGQHNASNSNLNNHNRHMRPNGAEYTTDSSSNNNLNPNRISSNTLNYHSLLYNWSSTLFTFATGLLPAQIAYDPVTPISKLLKQGAPLCLLFNALKPENAIDIVSSDDMKICKMNIYQFLSACKLHLNIRDDELFPVTMAFSDNTSHLLRVIRSVNFVLNLEPSFISPAIPDQLKITDARSKIVKELIETERRYVQDLETLLQYREDLINSECLLSEDITMLFPNLTDIIEFQRRFLVGLECNASVPSKYQRIGSVFIHAGVEGFKIYENWSLLQSFAIDLIRREALKMRNACKVIKDPYDLQNFFLIKPIQRLVKYPLLLSQLLKETDQSWPNYNELHQAYLISKEVALCINESQRRSENIQHLNDLKDKVVDWKGYNTRNVGELLYFNIVTVKDLLADGHSNEKEVHCYLFEKVIYFFKETSPKNKILGSKKMSNLSLTNALNNSMSNTTHPVQLSLNGIVYISKIYKILSSDLSPYFGAVQGHFLTLKWKGNKDTGGCVMKFRSEEHLKQWNNSIKKLSIDTGIDDIYATHNSSKSMSSISSSTSMLTGNTNRSSNTSASTNNNILNNNRNSDRLRSSSDSTTFMKKLRSTSSSSFTNLSNISYPPLPTEKMRSLSITSTNGATISSGNSTINLSVSSDIDYDTLISILVKKMNYSMSSENAFTTSNVSFKFKDEDGDFIRFQGDDDWAIAREMLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQM6</accession>
    <name>A0A1E3NQM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanine deaminase</fullName>
            <shortName evidence="8">Guanase</shortName>
            <ecNumber evidence="8">3.5.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Guanine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_13985</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>guanine + H2O + H(+) = xanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14665"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.3" evidence="8"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019522.1">
        <property type="nucleotide sequence ID" value="XM_019160166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQM6"/>
    <dbReference type="STRING" id="763406.A0A1E3NQM6"/>
    <dbReference type="GeneID" id="30176853"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="UniPathway" id="UPA00603">
        <property type="reaction ID" value="UER00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000022">
        <property type="entry name" value="Guanine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014311">
        <property type="entry name" value="Guanine_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051607">
        <property type="entry name" value="Metallo-dep_hydrolases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02967">
        <property type="entry name" value="guan_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271:SF6">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="9">
        <location>
            <begin position="102"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="491" mass="54408" checksum="150FFAD635897E35" modified="2017-01-18" version="1">MPTKTNAAYFADKISPVCHSALQYTAYYGTFAHTPSLGSMEVLPHTVVGVDQDGNIDFMVSGVKDASPEALVELTQQHALSALASKDIVLVDASKDNNQFFFPGFIDTHIHAPQFPNNGIFGNTTLLEWLEIYTFPLESSFTDLELATEIYTKVISRTLGYGTTTASYYATIHTDATNLLADLALKLGQRSFVGKVCMDQNSPAHYIETEEESKASTLEVIDHIRSRNPSGDLINAVLTPRFAGSCTDSLLSWLGNLRKKGNYHCQTHLSENHKEIEWITNMFPQYDHYTDIYYQNNLLSSKTVLAHCIHLSDEEMHILRNTGSGISHCPTSNSSITSGEARIRWLLNNHINVSLGTDCSGGFTPSILEVARHALLVSRHLVMKSKEDAEKLSSSDVLYLATVGGAKVLDIDDKVGCFRKGLKFDAQLISLDCKDSNVDTFAFQNPQWGVLPAEASHKRFADLIDKWLFTGDNRNIQKVFVNGRTVVDRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQT3</accession>
    <name>A0A1E3NQT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sm protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_19345</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019529.1">
        <property type="nucleotide sequence ID" value="XM_019162116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQT3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQT3"/>
    <dbReference type="GeneID" id="30178803"/>
    <dbReference type="OrthoDB" id="2020720at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="116" mass="13148" checksum="3DB27D03C3E33E21" modified="2017-01-18" version="1" fragment="single">VPKNTRLETLLNYKVRVTTSKNSTFMGTLLSYDKFMNLVLVECEEFRLLQKTKKYLDQVKNGDVDQSKVKEQKRLLGLVILRGENVVSVVAEAAPNANTLKPQLRLKKGKTSVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR01</accession>
    <name>A0A1E3NR01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein arginine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14063</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019615.1">
        <property type="nucleotide sequence ID" value="XM_019160204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR01"/>
    <dbReference type="STRING" id="763406.A0A1E3NR01"/>
    <dbReference type="GeneID" id="30176891"/>
    <dbReference type="OrthoDB" id="1368803at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035075">
        <property type="entry name" value="PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035248">
        <property type="entry name" value="PRMT5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035247">
        <property type="entry name" value="PRMT5_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738:SF0">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05185">
        <property type="entry name" value="PRMT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17286">
        <property type="entry name" value="PRMT5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17285">
        <property type="entry name" value="PRMT5_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRMT5 TIM barrel" evidence="6">
        <location>
            <begin position="35"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 arginine-N-methyltransferase" evidence="5">
        <location>
            <begin position="347"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 oligomerisation" evidence="7">
        <location>
            <begin position="520"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="688"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="688"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="792" mass="90136" checksum="C551915542CFCF7B" modified="2017-01-18" version="1">MSPRLKVQIGLKPQHQTAYRLQTEEAFLNENLVNGYSVFLLPITNQLYKELCKSYFQSWSKSPGEHEHSVDSLHVPNPELKHTDISTGKHVQHTVGLISSWIELDSDDIVISEFSFQAFYNEVSFANYLGIKTFMLSPPKNINNVQIFSSNIAKLLDLLPSIHITISLPMSQDLQDKNLSYFDSYSTWGMWNSIRLICNYHPSLSVSLGAPKSNIPSAILDRWLLEPIKFYLISASKFIPNAKCYPVLHKFNQLIIWKLIQYKALDPPVLMLHGVDRVKTTHSKHQNPSPNTMKEVDDNSKSTFLSYIQYIVNSSYKSDLLPRLHDFTIAALQKSGISKSTLFSPYILQSPIEPIVDNLENETYKVFEQDSFKYEQYERAIMKALVDLHSSWTSNSKPHILFLGPGRGPLIDRFFSALDLLSVSSKDFIITAIERNPNVMILLNQKNVKYWNSEVRIVNADSRGYAHLDYKARNPKFNMVISELIGSFGCNELMPECIDSISTADLCDKNCIFIPQDLHCYVSPVFCPKLWKFASLTSADKLYVPMLPEMEILLDFPKEVWKFESKVRQTTLENTATIFHSFNRHNSRQCRVSMEPTRKGLTHGLAGFFSSTLYDDVIVSNLPKPPGVDDAVSWLPAFFPIENPLNLYDGQEISLFIRRVCSLDSVWYEWSVECFLYMLAPSSNNNTNLTGTPPATPSPDRNQTSKLPPSLLMTRGDSEKANDETLLKQSVEMDRNQNQSIHTYDDASLYVTKSFNENASSASSQADYKVRVCTGLSRVHNLNGTGFKFRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR26</accession>
    <name>A0A1E3NR26_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_56778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019643.1">
        <property type="nucleotide sequence ID" value="XM_019163198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR26"/>
    <dbReference type="GeneID" id="30179885"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="156" mass="16584" checksum="87743B431C5272EA" modified="2017-01-18" version="1">MTGSSWLNRQSLSYPATPSSLNEASKGVAIFQGEGARKGGFLRRPFPPPAFLPSPVTPPAPFSRRPQHAGSLFPSSDPSPAQLHQPVEAQLSSAQLGSQSPSSLPSHHCLWVVTPPGGSPCFSCPLFLFLFLSPSLRLPVSPPPRLPASTLRPRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR33</accession>
    <name>A0A1E3NR33_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019659.1">
        <property type="nucleotide sequence ID" value="XM_019160221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR33"/>
    <dbReference type="GeneID" id="30176908"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="54" mass="6211" checksum="0D3732CF08E22CC1" modified="2017-01-18" version="1">MIRLLSLITQSLLASASYLNTKIPYHDKILISYPSIMIGFGILSQHIQWIFMTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR49</accession>
    <name>A0A1E3NR49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED17</name>
        <name evidence="9" type="ORF">PICMEDRAFT_124057</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019271.1">
        <property type="nucleotide sequence ID" value="XM_019159923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR49"/>
    <dbReference type="STRING" id="763406.A0A1E3NR49"/>
    <dbReference type="GeneID" id="30176610"/>
    <dbReference type="OrthoDB" id="5319830at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="628" mass="70628" checksum="55DEBA353CE431C9" modified="2017-01-18" version="1">MGDITIDAEEKAGTYPLPNIEYLNFNKSLIEGVHNDLPNPYYKQQAYIANERSDYAHPTENIIRSIIREYGPFSELTEEKLENLIEKDAAAMDVDGGNEDSSGNGVGRSEVLEISPSAGMEKEVEFYKARDEILTCVREALGSSTLTLDFVSLLLSSVRPAAGSSSMSPHLKESIKIGSLSCDRIVPNIADPDEKRRQHNIESMTLGRGLKLQSLQKSASLIKDSVQQLKKNLDDENTYWNEVLDVLKENELITTVSAPITLPGSAGGFSKQGSSLKQRRVLSVKYGYGDSGSDYFDNGIAILQKGEGGHIVFEKLNKNEREKTWGGPKIVSVKIFKKPTYANESPQLIGQSDSYGTLQREIIHEDESFVSKVKNSRFFIFENELFWHLMKEAAGLITVQVNVVDSCIKVDLVGCYLQLEALSIDSEEAKTPTPQLPANNRADQIIKFFRILLCANNYKNIQKLRIPPVALSKEVSAQKNKHGVLIRPVIMYTKHNHMIVQFKELLRSLLLELGTDDARATEIIERDLIVKKYVNDPNLLQQFQELKRCYNNDPFLRVYSKMAPSSLLILKNGNLKVWIELSSSYATLHISMNVKVTKLDSKETLLESTFNAKEDVEQCLSWILKQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR73</accession>
    <name>A0A1E3NR73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019687.1">
        <property type="nucleotide sequence ID" value="XM_019163589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR73"/>
    <dbReference type="STRING" id="763406.A0A1E3NR73"/>
    <dbReference type="GeneID" id="30180276"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="51"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="36581" checksum="D543A45A2D11C9DA" modified="2017-01-18" version="1">MSSKSLLTSVQIRRTSSLHNANIKHVHLSPQILDYDLFTPSIAPKANSKMPPMIFLHGLLGNRKNNRSAAKLLAEQLQTQFIVPDLRNHGTSFHARPMNNKAMSDDITKLIDSLPSTIPRHRGFVILGHSMGAKVAMIHALRFPKLIKGVISIDNVPYDNSEDSYVEFEKFHIGLRTLDWCVKAHPDWSLPDMKAYLMKWVEPSEKIINFWLTNVVNKNGLLVPKVPFNILNESIEDIMQWKMQEYGDLESLAGQKVVAPLLLISANYSKFVGKDVHTHAISKYFQDFIVKPVDSGHWVVTERKQEFTKIVIEWALSKFGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRB1</accession>
    <name>A0A1E3NRB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADH-ubiquinone oxidoreductase 12 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFB10 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019733.1">
        <property type="nucleotide sequence ID" value="XM_019160251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRB1"/>
    <dbReference type="GeneID" id="30176938"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019377">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039993">
        <property type="entry name" value="NDUFB10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13094:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13094">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE PDSW SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10249">
        <property type="entry name" value="NDUFB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10892" checksum="221C194EC9C14558" modified="2017-01-18" version="1">MAESEFKHAPSVSFDEIDYRNEVDLKKAQESMLKEQFVKIEVVKIVRKALENCFKVNGPNGYEDCREIADKYMALLPESRAQGYLGYQRNDPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRD2</accession>
    <name>A0A1E3NRD2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019800.1">
        <property type="nucleotide sequence ID" value="XM_019160277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRD2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRD2"/>
    <dbReference type="GeneID" id="30176964"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061245">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF456">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="553"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1034"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="801"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1128"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1044"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1072"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1099"/>
            <end position="1110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1176" mass="129923" checksum="7FC8FC32007656E8" modified="2017-01-18" version="1">MASFFKKINPNSKSPKPGAVYDSPPTASLPSPPVNQLKGEFNKRKLFPHKEKDPKYRSVSYDVPAPGARNTENYDSSNPNDTVGSASSNTTSHSHRTSNPPSRNTSHTHETRHEQVPAQTQMHTPNGTGTLLHPTAGGGTAGTSNNSNHSYGSNNSNNSGPAVTPWRKKKLYNSPFPRFGHAVSSLTSTSGTCYLMGGLSGNDVYGDMWVIEPIRKQDSSGTDSPYIASPIENPQKIPSPRTGHASILIGNAFIIFAGDTAINYDQSLDNKLYFFNISSLKWTITSPEGPKPCGRYGHQIAVLNFEISPSKWSSQLFVFGGQLNDQYFNDLWRFDLSKFKDPNNQWVRIHAKGDLPPPLANYTMTACNDRLYLYGGHNSGHINDQLYCYDALKNEWAICKLHGISPPALYGHSTTIFGTLLLIYGGRLPNDTNSGDLFVIDLSTFRCWRLQPNLPYSPGPRYGHSITVNPVDEKIIIMGGDIYDNDFNGIDDSSIGMIDEAKFSLLSSVIYECDIKLLEKFTDKSSMAISNSSEAEVSSVPFSAVTAATSNKAETGSATLASDSRTTFPTSFGRDKVRKSQLNIDLNDTSPSQPPHSPTSASTGPLVSSTPLHSADEEDYVSVDESVVHGTAKRPSEKLYMTSESPFSNSDTRNLSDSTQIKHDLDEKSRNHEVVDNEIFESENIDNIKNESTFANTNVQTDPVSDLIYKTPENDETSKLKKLTAISSNSNTTSTSVSNMTAKSVDEDEVYDDIPSNFATPSLQQFSTMPFSNSATPFSPALVERDNVKLMRLIQMVNDVKAEMKQSVSQANSQIVTLEAEKKELLEKLNAESRGNDSSMIEKNLQLESFIKDELSTIPALNSLIKEQQETINRLSAKVQGEELLQEKIYNLEAQNSSLNSKLENFFVIHGVTAVGEEDQSKDVSKAIKPTNFESVTTKVDALLEKWNNAAPHSGVLKEIDHLKQTNFNLSKQIEDSSSKFKEFEELYTESRNSLNKSHRALLLSQSETNKLKDQIKVLSNELEELKLKKRVFSNSNPRKPSYNRSLNSSTNANSTNKAVSSHVEGVEPNDLNGSNHAVNEGATNQSNTRNAGSVTEIDNFDGENGEIDESTSQHDISFVDDRYEIRIKDLEANLFIVSQERDQMREELISLKKQIYNTRNNASFSSSPVVSSPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRK9</accession>
    <name>A0A1E3NRK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_70333</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">COPI-coated vesicle membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="13">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019829.1">
        <property type="nucleotide sequence ID" value="XM_019163613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRK9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRK9"/>
    <dbReference type="GeneID" id="30180300"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="5 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="93"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="320"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="17">
        <location>
            <begin position="594"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="818"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="818"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="841"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="851"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ48716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="877" mass="99032" checksum="58141B1949866BAB" modified="2017-01-18" version="1">MKLNIKKVSSTRSDRVKGIDFHPSEPWILTTLYSGKVEIWSYETKTLVKSIDVTNVPVRAGKFIARKNWIVCGADDFQIRVYNYNTGEKVAQFEAHPDYIRSIAVHPSRPYVLTSSDDYTIKLWNWENNWKMEQIFEGHQHYVMSIAFNPKDANTFASACLDKTVKIWSLGSKVPNFTLTAPESKGFNYVDYYPHGDKPYLLTSSDDKSVKVWDYQTKSCIASLEGHISNVSFAVFHSELPLIISGSEDSTIKIWNSNTYKLEKTLNYRLERAWCVSTKKNSSLVTVGFDAGNVVFQLGDDKPLLSMDQSGKIIWCKHNEVYHSVIKSSDAKAEDGEILNLSQKEFGSVEVFPNSLVHSPNGRFVAVTGDDEYIIYTSLAWRNKMYGQALDFIWAQDSNYYAIRESKTSIKIFKNFKEKPNGQINLLYPAEKIFGGALLGVKSEGSVSFYDWESGEIVRTISVDAENIYWSEDGGLVLISSNESAYALSFNRDVFQESLDNGTIDPEEGVVESFDVAFEVDDQILTGKWVGHVFIYTSASNRLNYLVGGSIYNLAHFDKAMFLLGYMSRDNKVFVCDKDMNVVSYYLSLSVLEYQTVVMRGDLEYADSIIGNIDERDINKIARFLEEQGYTEKALEISTDLDQKFDLAIETSNLQLAAEIATKEDSVHKWKKLGDSALSHWKFDLAIKSFENSKDYQSLLLLYTSFNDVEGLKKLSKSCIEIEKFNSAFAASWAAKDLEACIDILKRADRFTEATFLSLVYKGDSKQVDEITKLWKDELSKNGKESIGSRILVPSEHPEAFPSLKTITETLIDIDGPPEQEPVETEVEAAAPSEEVVIENGHAEEPIDAEKESDENDEDAELVEVAEVAEDAEEDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRN7</accession>
    <name>A0A1E3NRN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14323</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019900.1">
        <property type="nucleotide sequence ID" value="XM_019160325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRN7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRN7"/>
    <dbReference type="GeneID" id="30177012"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF4">
        <property type="entry name" value="SECRETORY 24CD, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="7">
        <location>
            <begin position="260"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="8">
        <location>
            <begin position="336"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="10">
        <location>
            <begin position="581"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="9">
        <location>
            <begin position="677"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="965" mass="108152" checksum="A132326220081F56" modified="2017-01-18" version="1">MSMVNDFVTGQQNLLAQELKDLSLNNENSHQRDLAKEQNGYHISQQGQNTQASASAKKRRGHHAFHNLTSPAPQAYEHEHVDPNEAASAVGLFPPQFSNQYDMSSGGSGSQNANSNFLQNNFGISPQMSETSRNDALVQKNDNGFSNDIYNINQATVAEDLSVPGIRNHLNEIYKEKEFLSFEHVSPPLAGTEYKAIDQANSIPQFSRLSMYSIPFCEELREKSKIPLGFCLRPFADYVPQSEVPMKVPQIKIQEGEVVPRCRRCRAYLNPAMQHNGRNMICNICGFASPVPEAYASTVNFQGVRDDYHLRPELHTGVVDYIVPKEYNLNENEDLSPLHRVFLIDLTHASYKNKLVETVCSAIRMAIYREDGSSNLPPGTLISIMGFDNNLHFYKLSPELQQTTVSIVTDLEDPFVPFNDGLFVDPVESYNTIDLTLTTIEQNARYLTPEPALGSALTAAGLLLKELGGGQVVAFLSTLPSFGPGASLPRNNNNNKLDADFVKETLTPASKYYKNLATEFVKNNVGLNLFVASNSNVDLINLGTLAVNTGGTVKEWLPFNVERDDITLIYELKKVIQYVAGYQSQLKVRCSHGLQVNKYYGPFATVNGDSAPNIPIVSGDTSIVCDFIYDSKLDTKKDVHFQAALLYTTSEGVRKVRVINNILSVTQRISDVFDFSDQDTIVKILIKRAIEKISTANLVALKSSLMMQCSEIMAAYKYYIARHNAMPTQLVLPQSLRTLPMVILSILKTRAFANKTNFPDLRVYSFFKLSQYDLTKLSAYLYPLLFCIHSLEEGEFISNEQTGMMNLSQSIPVSVGNLAFGGAYLVFNGDRTIIWLHSDVNVVLLQDLFGSHIDSLDKLTTYVSQMPVLDTYISSQVRNICDYLAKHFNSTEKQSVEICRFRTDPNEQEFQSMFVEDKSLDLVWSYAEFLKELHKNIENKTNNLNSLSSKVDNDGENLSRRFGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRS5</accession>
    <name>A0A1E3NRS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_20727</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019376.1">
        <property type="nucleotide sequence ID" value="XM_019162171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRS5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRS5"/>
    <dbReference type="GeneID" id="30178858"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01389">
        <property type="entry name" value="HMG-box_ROX1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000041">
        <property type="entry name" value="HMG box family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF161">
        <property type="entry name" value="SEX-DETERMINING REGION Y PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="79" mass="9527" checksum="6C0A936F323D39CA" modified="2017-01-18" version="1" fragment="single">IPRPRNAFILFRQKHHLALIEEGNQVKSNPEVSKELGRRWRALEPAEKDYWNNLAEEEKRLHAEKYPGYKYTPKRNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV0</accession>
    <name>A0A1E3NRV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial import receptor subunit TOM70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_13995</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019531.1">
        <property type="nucleotide sequence ID" value="XM_019160176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV0"/>
    <dbReference type="GeneID" id="30176863"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="355"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="531"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="34"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="603" mass="67790" checksum="EC6A82BF1A1DB823" modified="2017-01-18" version="1">MSFLQKNKVAVTVTALLGVGTLAGIVYYMKYSGASKDTSSGSSDASKKKRKNKKKKGKSGSSSGQTTSSSSSAPIAVPEEDPEKSVYPVNPESKLPEVTAEEVSKLSAGEKDKWAIALKEKGNEYFKAKQYEKAIIYYTNALTCKKDHIFYGNRSACYSALKQFDKTIEDTTAALEIKPDYSKCLLRRALAYEELGRYEEAMFDLTALSIYGRLTDQSCESILERVLVKLANKMNEEVYSHLPKELPSSSSISSFLGAFVKEDIDLDLSKYEKDSGIYFFISALIEINKDTDEGYENADSLLNQSIQRFEKGSYDAKDKKLVAIAYEYMGIFSFLKTSDNASSYVEKALSIYARPRMYVVLALIAADKGDYLSADAEFTKAIEMNPEDPNIYYHYGQVYYLIGDLTKAQSNFEKAKSLNPKNVFAHIQLACITYRHNDVQKCFEMFNQAKSIFPTAPEIPNYLGEIMFDKGDVENATKQFDISAKLQEAMPGNNIGVLPLINKSVIFQKTNEFDKCIEILEKAVKIDPKSEIAWTNLGQVYLLTQKMEKSMECFERACRLCRSSEDRKQSIALLESAKCQLKVKEDPVLSEKVREIMAQYGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV3</accession>
    <name>A0A1E3NRV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Programmed cell death protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019935.1">
        <property type="nucleotide sequence ID" value="XM_019163636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV3"/>
    <dbReference type="GeneID" id="30180323"/>
    <dbReference type="OrthoDB" id="10252486at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16184" checksum="61903A447C95CCEF" modified="2017-01-18" version="1">MDDAELQAIRAARLQEMQRNAQGGGGSGSGVSGSGDDARDKANNVNEGILNQILDQSAKERLNRVRMVKPERVLGVENYLVRLVQSGGLRAKVTEADIVDILDKIAQEERKNTTTRIKFERRDYVDANAGASRRSEDDSEDDFFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS11</accession>
    <name>A0A1E3NS11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019982.1">
        <property type="nucleotide sequence ID" value="XM_019160354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS11"/>
    <dbReference type="STRING" id="763406.A0A1E3NS11"/>
    <dbReference type="GeneID" id="30177041"/>
    <dbReference type="OrthoDB" id="3994639at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="75799" checksum="94B728B801927C75" modified="2017-01-18" version="1">MPMKEKQNISRLKRSVRELFSEGSARSGHDNVKKHEPTADTFNALFTKETFVISEEDLRMINGDLQKQMVIDEFRELVSDDVYPEEKVAGEYDDDSDYGGDHNEKDSAFANKVSVISEDEDEDEQHENYGLDESDTGFSDFEKQEYERYIDNPDKHNQVLPDFFGDSRTKSKPGQGHSIFDDTGLDSDTELDNTHFLYDPPLKYKEKFLMYMLKSSPLLIVGLLLLAVSSFVTKDSLENLKTLNPFASGFNPSPFLSHKIYLLEEDVKSLRRLEGLGEKVSCIENEFKILEDHISKLEFSISSKDMIDTSNDVVSPLFEKLNELERKVDALSKDVTRDIADKKFLKEMKGQIVRYSGDHVGHANQAKDTKLHKVSSKFFNIANKCHVSLQLTSYPPLSQYKREKRRKTIQSRIIHGFPDFMKRMLNPATSQAGNSVVKTLADIKLLSPSNSPRNVLLEHPSLFWQSVASAVPIYLTVQAPEPIKIHELGVYHSRQPPNMNHVDDAAKADVRKRWFNTAPKNVEYLVRPIATEQTAMKEAMGAFYNQDLKFNISKKVKADGLAGWVRVGELKYDINSNRAYQPLIWNDEVKLEIRKWNVLDFMVVIHSNWGDEVVVLDTIRIFQLEDTKSFDYDKSRVNPDGYGDESFAEDEVIYLGEEKPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS13</accession>
    <name>A0A1E3NS13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_14368</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in rRNA-processing and ribosome biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 90S pre-ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat NOL10/ENP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019949.1">
        <property type="nucleotide sequence ID" value="XM_019160340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS13"/>
    <dbReference type="STRING" id="763406.A0A1E3NS13"/>
    <dbReference type="GeneID" id="30177027"/>
    <dbReference type="OrthoDB" id="273340at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000537">
        <property type="entry name" value="Ribosome biogenesis protein ENP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056551">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040382">
        <property type="entry name" value="NOL10/Enp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056550">
        <property type="entry name" value="NOL10_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23098">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23097">
        <property type="entry name" value="NOL10_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nucleolar protein 10-like N-terminal" evidence="14">
        <location>
            <begin position="20"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolar protein 10-like second" evidence="13">
        <location>
            <begin position="373"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="12">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="506"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="655"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="554"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="662"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="687" mass="78446" checksum="2D42288212EA1F39" modified="2017-01-18" version="1">MVLKSASTNDISVYQISGTNVSKSLPDWLERKRKRSLKNDVDYQNRVELIQDFEFSESSNKIKVTRDGQYAMATGTYKPQIHVYDFSNLSLKFERHTDTENVDFLILSDDWTKSVHLQNDRSIEFQNRGGRDHKLRIPKFGRSLAYDDVSCDLYVGASGDEIYRLNLEQGRFMKPFQIENDRGVNSVDVNTTHGLFSAGLEDGTVEFWDPRAKQRIIKLQIAEAKESGSEISCVKFANNSLNFACGTSDGLTMIYDLRTSHPLITKDQGYGFGIKKIIWLEESGNENMNLILTSDKKIAKIWNRDNGKPYAAMEPSVDINDIEHVQGSGMFLMANESIPMHTYYIPQLGPSPRWCSFLDNITEELEEKPSDTVYSNYKFLTKQDVAKLGISHLIGTNVLRAYMHGYFIDNELYDKVNLIANPNSVAEEREREIRRRIEKERESRIRSTGAFTNTKIKVNKEYVGHLQSKLGSEAAEGVVNDDRFKEMFEDPRFQINEESHDYKQLNPVASSLAGPKPLTAAEESDEERMAMRNKNGANDSDSGSDSDSDSETEEKEKELARQKLKEQLKQKQEKKKRERAEAAQYMSKMKSINKNDELSLDGERNKVVSFGKQAKLQGESSKVRKEQKANTRIRKNAIGEGEITFIPTKKTAKMAFEDQEENSERRGKAKQVVDGRRRASKNVFRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSC4</accession>
    <name>A0A1E3NSC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <ecNumber evidence="3">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70581</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020114.1">
        <property type="nucleotide sequence ID" value="XM_019163680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSC4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSC4"/>
    <dbReference type="GeneID" id="30180367"/>
    <dbReference type="OrthoDB" id="184182at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000002">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [ATP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015994">
        <property type="entry name" value="PEPCK_ATP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00532">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="560" mass="62229" checksum="2AD3FCC4E04B7C54" modified="2017-01-18" version="1">MAPTAVDIQKEYRENVSTEHQAPFNKNERKSSISSKLGLNPDAKIHYNSAVPILYEDGLKEKGTTISSSGALIAFSGTKTGRSPKDKRIVDEETSSEHIWWGPVNKKVDENTWNISKSRAIDYLRTREKVYIIDAFAGWDPRYRIKVRIVCARAYHALFMKNMLIRPTTEELKNFGEPDFTIWNAGQFPANVYTKGMTSSTSVEINFKSMEMVILGTEYAGEMKKGIFTVMFYLMPIKHKILTLHSSANQGVKEGDVTLFFGLSGTGKTTLSADPHRELIGDDEHCWSDHGVFNIEGGCYAKCLDLSAEREPEIYNAIRFGSVLENVIYDPVTRSVDYGAASITENTRCAYPIEFIPSAKIPCLADSHPKNIILLTCDARGVLPPVSKLTNSQVMYHFISGYTSKMAGTEVGVTEPEATFSACFGQPFLVLHPMKYAQQLSDKMAEHNSNAWLLNTGWCGQSYVKGGKRCPLKYTRAILDSIHSGELAQSEFETYPTFGLQIPKSCPGVPSEILNPSKFWASGSQDFNSEVTNLAQLFMENFEKYSAGCTQEVVAAGPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSE0</accession>
    <name>A0A1E3NSE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70554</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VAM6/VPS39 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020077.1">
        <property type="nucleotide sequence ID" value="XM_019163671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSE0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSE0"/>
    <dbReference type="GeneID" id="30180358"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019453">
        <property type="entry name" value="VPS39/TGFA1_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF49">
        <property type="entry name" value="VAM6_VPS39-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10367">
        <property type="entry name" value="zf-Vps39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated" evidence="5">
        <location>
            <begin position="534"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated zinc finger" evidence="6">
        <location>
            <begin position="872"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="912" mass="105381" checksum="3AB1D11803E49E43" modified="2017-01-18" version="1">MAALFNKQLGSSLLKESIKEVPIAIATADKITYVATPTGIYKTATGKKLDKIYDSIVKIREVYCIPHINILIIVYDDLVEFRNVDNIQSKEKPHYQSINIRSTSVSIWNNISSNHVVSRDTNSDMISLATVVLDQRQRVSYGPVTPSDTDSPSSETMNDPPEPTQTLLPKSSTGELSDLISCVALASKNQVVVLKWVNHSFVDRYNLYMSNTQFTQFIAADQLLCISSSNPNDLVHVNLTTTKIKHNDLFKLLNIKSSMMSFKHSNQLANLHFFNNRIVFLKYDMFIALAYPNLEKLVHVKITSTNFKASKIEFPFIFLMYGNSIEVRSIADFKLFQTILLDSIIKIDYHDTNLSVLTKDRITILEMIDYNQILELLYNEKDYDNAILLVENLDISNFTTDPDSTTQNVAQLKFEKLRKFQLLKAIFLLQSSDDQFSKAIDLFAEYLGSPKQVISNLPQGLKQVIESGELPPSYTHKSQIYQLIRFFTDSRRKLIRLLDNTFTVFKYNNLEITLSVYKDDDSDFSVEKNLTLLDNYLFECYLLVNTKMINPFLRSKTFCDFKMVEEKCKELDLLDQLITFYYTRGEFSKSIELLRQLRKIDELVKFLQKLIQLPQVPMKLITDNLQVIVDSNSNNFDLLLLNNNIDYSNVDYRQVVEYLTRPNLKNYRLRYLEYLILHQKVTSLELSNELFDIYFADIEANHEKIAKLYSVSSYNANQILKKLRSLPSSHISQKLMIPPLMKLGRYDDILNIYVYGLNDIESSIDFCLHVRDIKNDSLSRGLIFKVIDLCLKKKDHPSIINYILNNPALDYIDFEEILIKLPNDISVNLMSSFLIMNLKKMNSTNKSLIVKNELLKVNVINMKLDKLSLERKMFRMTQSSICVHCGRNFNKSEILCFHPNGDVLHYKCSKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSG3</accession>
    <name>A0A1E3NSG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_19512</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020125.1">
        <property type="nucleotide sequence ID" value="XM_019162125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSG3"/>
    <dbReference type="GeneID" id="30178812"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF95">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="291"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="231"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="651" mass="73850" checksum="2D630E54DDA333F0" modified="2017-01-18" version="1" fragment="single">NDPDMKLLDLYSMKKFIDVDTLMDTYHKFDSSVLLIDYRPSPSFKSDHISLFTNIINIDPLSVKPQYTVQDVIDHSLLLSTFKEREIFMNIAQYELVIIIDQSSIHAKLAQDILRFIAILDEKNHDSKYRLKRKPITLDGGFDEWVYFMNNQATRHHTTQQALPKSPMTMNDIPNLQQNYVHTKPFLSRSPSPTGLFPTLPSSHLTSKFGGGLSKQQLPQPTMLVPISSPLTRTRSPSPSPVFRPTVKVAPSTSSSSPIVHRSNNTPVLQVSSVNTPVRSASPNKSPFLVSGLYNLGNSCYMNASLQCMMGTKELTNYLLQDIYLKFVAKDSKLGSRGKLTKEYQTLAKTMLANTLKHVATNPKNFKKVVGSVNSAFNNCYQQDSAEFLHFILDTIHEDLNWSANKESLPAITDEDEANREILPMRLASTIEWERYLKTDYSTIIEIFAGQYASRLECGNCHKTSTTYIPFNMLSVPVPSQKKELNLYDCIDSFVSPEILSGDNAWKCPRCKGSFSTKKELTITRLPAVLIVHLERFSLSSGPNLTFVKNTSKINIPLKMSMKQYWPQIQDEQERQQLKKFPTRGQEGEWEYSLYGIVRHYGTLDGGHYISEVKKNGTWVKFDDDKVKVSDTMGKKGESDGSAYILFYEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSR4</accession>
    <name>A0A1E3NSR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020225.1">
        <property type="nucleotide sequence ID" value="XM_019163704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSR4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSR4"/>
    <dbReference type="GeneID" id="30180391"/>
    <dbReference type="OrthoDB" id="5423111at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052649">
        <property type="entry name" value="NCE102-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165:SF1">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="170" mass="18596" checksum="58610487D24ECD3A" modified="2017-01-18" version="1">MLSYGDITLRIINFIFLTIALGLTASLAATTVDHHNPQVSYCVFAAAWGILSSSFYGIVAFFVAFLASPVFLAIFDFMNFVFTFAGATALAVAIRCHSCSSQDYLDNNKVTQGSKDRCRKAQASVSFLYFSVFVFLFSLVMQAMNILKNGFFSTSVPKTPRTGVPTMAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSV7</accession>
    <name>A0A1E3NSV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_14663</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I LYR family. SDHAF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020302.1">
        <property type="nucleotide sequence ID" value="XM_019160478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSV7"/>
    <dbReference type="GeneID" id="30177165"/>
    <dbReference type="OrthoDB" id="3994406at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20268">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045295">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="5">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="79" mass="9747" checksum="B12C362F54641EE4" modified="2017-01-18" version="1">MVKKFSGLQREVLDLYRNCLRSLHQKPKENRRNWVNYIHEEFGKYRNVPRRDFGTIEHLIRVGHRRYEMYSDPSIKNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTD6</accession>
    <name>A0A1E3NTD6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14748</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020389.1">
        <property type="nucleotide sequence ID" value="XM_019160510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTD6"/>
    <dbReference type="STRING" id="763406.A0A1E3NTD6"/>
    <dbReference type="GeneID" id="30177197"/>
    <dbReference type="OrthoDB" id="6287070at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF722">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="109"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="360"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="744"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="822"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="959"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="377"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="392"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="750"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="830"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="959"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1014" mass="113166" checksum="7FB61E972A2FFC30" modified="2017-01-18" version="1">MLASSQYNGVLLFDDAVPKAQIKLSTERKSLSKQIGALVQSNLGKTVAKDPSNQRLINVIIVLLNLDLNTHANVLSGYKFFRLYQLSSEGKMITCKGEYKNKHKEISLVGMDNYSNTTCYLDSLIVALFYSNSSFDFLLDKTIDPNLSSEFQQQVERMKTVLRFIVNLLRAGEHINTNIMYQLLVTLDSLGCDIALSRKQQDALQIFEFLAESLSLPLLTLKLDIIHTGKLNVNDDLRLIGERSLLISISSSATSNDSPITLEECLNTYFNNSITVRRHIDQQLGSTSGNKIRFHNSDAICSSGESLAQNKDEINEYEKLGILTSSSSSKTSVPIPSSISTSLTPSISAFWPNTLQTPLPSSDASISAPDQLPITPQQSSQDSSVSPTGSQLTSTKSILTRTPSSSKSRSDFETPTGSQSKKSLPNTGSSITDLSPDNNNLTATDSATLDSVNLIGSFRNVTEKLERQRTRSSTLASVLNNVQSINPKKLTRRSSSISNTEVSLPAWMYLQLLPYYTDPEVKLTVENHEEYYRRRISRSKTIDSAQNIPVGLQDKTSSDDLFHTDAESYFGRRFANRRPMVPICLKRYIWNERGQSVKIQRKVTIPEIIKYPYFIAEDKKKPGYIDFRRSYDHQAPRGSFMLVLQSCVCHRGNSTNSGHYVSLVRKRRFDVYHMDANNEWLLFNDIEIGKNKAQSCTFKEAMDKENPYILFYEIYEIKDDGYQTPIGAKESYWNRKQSVNSGFSGYSEQTLHEEPQEENKSTNSTKHMMHLSLAGLTLSKSRSKNIGTDMDDVLDEYFWYGASSDRNNSNFKSNYSLNTNISGRKEESSTEQASSHSASSTTSIPYPEDESVPQVHVMEVEESTSTDIDNSSEFENGKIEYSAGFLDDNNDKLNNSLPFNDDDVDFARKRQFLKVSSGIASNEAAIGSRAGLYTSISHREQKSNLLDVKLTQSHKSVGTVNTGKTVHNDEPSGQSISDGAVNAAAATMTAKTTKKKSSLARKGTVKKLFKKVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTQ2</accession>
    <name>A0A1E3NTQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial succinate-fumarate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70961</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020532.1">
        <property type="nucleotide sequence ID" value="XM_019163784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTQ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTQ2"/>
    <dbReference type="GeneID" id="30180471"/>
    <dbReference type="OrthoDB" id="204711at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005469">
        <property type="term" value="F:succinate:fumarate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000021">
        <property type="entry name" value="SFC1p Mitochondrial succinate-fumarate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF2">
        <property type="entry name" value="SUCCINATE_FUMARATE MITOCHONDRIAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="13"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="111"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="215"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="34818" checksum="6AE74D803B9EE0E1" modified="2017-01-18" version="1">MSSASQKSNQRPKSTVIDLIAGGTAGLFEALCCHPLDTIKVRMQLYKKATTAGVKAPGLFATGYNIFAQEGFFALYKGLSAVCIGIVPKMAIRFSSYGFYRSLFTDEKGNISGPFTFLSGVMAGVTEACIVVNPMEVVKIRMQAQHNSLRDPLAKPKYTNAPQAAVLIVKEEGLKTLWRGVALTAGRQAINQGANFTTYSFLKSRLQDSQNTEVLPTYQTAVIGFISGAIGPFCNNPLDTIKTRMQKETGISNESNLSRGLRIGKNLIKESGVTAFYKGIIPRVIRVASGQCVVFPVYEFFKGFLYEVAGYDKPEKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTQ6</accession>
    <name>A0A1E3NTQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14919</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020587.1">
        <property type="nucleotide sequence ID" value="XM_019160597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTQ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NTQ6"/>
    <dbReference type="GeneID" id="30177284"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF3">
        <property type="entry name" value="HOLOCYTOCHROME-C SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="280" mass="31741" checksum="78B73693F4C9A075" modified="2017-01-18" version="1">MGFFWASTPSPAFATDAAASAIYNQSPPPACPMHNKDLPVACPVKKTGLSSDDEKLNPLTNMPYDLSTKKQPGQKIDLPTERTLSTIPRGEDQGQGVWEYPSPQQMLNAMLRKGKGDVPEDAVESMVNIHNFLNEGAWEEIEQWEEPYTQQTHIQPRLLKFTGRPDAISPRARYYNFMSYFFPDKYGSELPFDRHDWTVLRSDGAGNWKEVRYVIDYYSGGEDQFGLPAFYLDVRPGLDNVDNAVQRFNHWKEIMRPTWEKAMGHSPEAGSTLVSAAEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTT1</accession>
    <name>A0A1E3NTT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Plasma membrane ATPase</fullName>
            <ecNumber evidence="11">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_70994</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020562.1">
        <property type="nucleotide sequence ID" value="XM_019163794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTT1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTT1"/>
    <dbReference type="GeneID" id="30180481"/>
    <dbReference type="OrthoDB" id="116380at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000042">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="323"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="694"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="721"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="757"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="796"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="827"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="860"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="13">
        <location>
            <begin position="72"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="920" mass="99871" checksum="B9DAEE4A9012FCB0" modified="2017-01-18" version="1">MSGAVEQRPEELAPPQSAVVDEKKDGVVPGSGAAGAGGDESDDDEDIDQLGGDEDGEEGDSDEEDDEKHSSSYKEVPDEYLHTDPAVGLTASDASKRKKKFGLNQMAEEKENLVLKFCMFFIGPIQFVMEAAAILACGLEDWVDFGVICGLLMLNASVGFIQEYQAGSIVDELKKTLANTATVIRDGLPTEVPANEVVPGDILRLEDGTVIPADGKLVSEECFLQVDQSAITGESLAVDKHFTDPTFSSSTVKRGEGLMIVTATGDNTFVGRAAALVNKASGGQGHFTEVLNGIGTLLLVFVIITLLLVWCACFYRSDPIVRILRYSLAITIVGVPVGLPAVVTTTMAVGAAYLAKKQAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVAGVEPDDLMLTACLAASRKRKGLDAIDKSFLKSLINYPKARAAMKEYKTLEFQPFDPVSKKVTAIVESPEGEKIICVKGAPLFVLKTVAEDHPIPEDVLEAYQNKVEEFASRGFRSLGVARKRGEGHWEILGIMPCMDPPRDDTASTVAEARRLGLKVKMLTGDAVGIAKETCRQLGLGDQIFDAERLGLGGGSTDMAGSELADFVENADGFAEVFPQHKYNVVEILQNRGYLVAMTGDGVNDAPSLKKADTGIAVEGASDAARSAADIVFLAPGLSAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWIAILDESLNIDLVVFIAIFADVATLAIAYDNAPFAQRPVKWNLPRLWGMSIILGIFLAIGTWITLTTMFLPRGGIIQNFGSIDGVIFLQISLTENWLIFITRAVGPFWSSIPSWQLSGAVFIVDIIATCFTLFGWWSQNWNDIVTVVRVWIWSFGTFCVLGGAYYAMSESEKFDRLMNGKSIKKPHNNRTFEDFVATMKRVSTQHEKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU00</accession>
    <name>A0A1E3NU00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2 7">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71009</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the esterase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2 7"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020579.1">
        <property type="nucleotide sequence ID" value="XM_019163800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU00"/>
    <dbReference type="STRING" id="763406.A0A1E3NU00"/>
    <dbReference type="GeneID" id="30180487"/>
    <dbReference type="OrthoDB" id="420518at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="295" mass="32983" checksum="51443CFAF8792555" modified="2017-01-18" version="1">MGRFETKDTVAVCGGKLLKLSHASEYTKTSMDVNVYLPPQYEASGAKKLPVLLYLSGLTCTPQNVSEKSFLPYFASKHGFALVFPDTSPRGAGINGEDESWDFGTGAGFYVDSIREPWKKNYNMYSYVLKELLPLVSEEFGQLDVLENCSITGHSMGGYGALMMYMRNPGRFKSCSAFAPIANPSVVPWGEKCFGNYLGEDKAEWYKYDPCHLVKAYAGNPQVADEKILIHVGTKDVFNYRDHQLRCENLVEAAKGTKLAGSIDLNVVDGYDHSYYFISSFVPEHVEFHLKYLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUA0</accession>
    <name>A0A1E3NUA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020689.1">
        <property type="nucleotide sequence ID" value="XM_019160632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NUA0"/>
    <dbReference type="GeneID" id="30177319"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27337" checksum="A00B3B33C3794882" modified="2017-01-18" version="1" fragment="single">MKLALKLSDVGNFKRILGFVLSVRKQCIFKFKANELNIISVDRESPLIWGTIGSANFSRFDVIAKDECIGLELNVEPLFQIMKNFEKAPVTSDLIIKLQRGEESNTPKDNSSKRKRPVFLHLSYNEDITCTSEISHSFSIPVSLLRGKLIERIQMPPIHNVELIADMNQTLISFFMRIERYKAIDNINVVMNRLGEIKIELKDEGKKISLKWKSLLDTCSPEEVDALTRTDTETPATHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND12</accession>
    <name>A0A1E3ND12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_179420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015163.1">
        <property type="nucleotide sequence ID" value="XM_019161820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND12"/>
    <dbReference type="GeneID" id="30178507"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23274" checksum="D3229A8DB0F22AA0" modified="2017-01-18" version="1">MTLKALGYNIDGQAESFISDQKLAHYSKIPQRAVFRGQIFGCLLQIIVTLCVINWEISNVKGLCTPKQESRFSCPQEKYFPKQTKHFYPTLIIGGMLVFAPYNLSYYYPAFVLGYIFNHFIKGRYLSWWERYNFVLSAGLNTGTAFSAVIIFFAVQYHPVVLSWWGNNVPYAGTDGQADGYQMSLLDPASQPDGYFGLRLHNSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDC9</accession>
    <name>A0A1E3NDC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18582</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015237.1">
        <property type="nucleotide sequence ID" value="XM_019162069.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NDC9"/>
    <dbReference type="GeneID" id="30178756"/>
    <dbReference type="OrthoDB" id="412781at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034512">
        <property type="term" value="F:box C/D sno(s)RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034513">
        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034463">
        <property type="term" value="P:90S preribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000464">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05708">
        <property type="entry name" value="S1_Rrp5_repeat_sc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000065">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000159">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="113"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="242"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="328"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="506"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="586"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="686"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="783"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="879"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="987"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="1071"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="1155"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="1243"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="1555"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1321"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1390"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1321"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1414"/>
            <end position="1424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1698" mass="189043" checksum="A9E549CC22B8F40D" modified="2017-01-18" version="1">MPPKRRREDPDAKEQKSLLSSAAEIAFPRGGASILTPMEMKEVANEAKRDVLFEQKSKSEASETVSEQPLKKRSRGNKKSKSLNLLSKDEQEEEDAKKSITVDSLSYNILNPGSYILGMVQEINKMELVLSLPDNLVGYVPITNICAELVKLLEEFDDQEESDSESDSESDSGDEEKDGRISKNAKQVKEFPLLSDRFTVGQYLRAKVVPNTNEKAKKRIELSIEPEKVNAPMDTKEDFIADAIVQASVVSLEDHGAILKFGKKQKLSGFISKKELSSFSPNVQIGSVIMVYISNVNPRTATCKFPVAVVKKQPMVSSVSTIDGILPGMLVDAVVEDVTSDGLVCKSHSLCPASLSLQHLGPLQTFEELKHNYAVGSTIKARVIAVYMKNGTTKVSLSALPVHQTLTYPGKDALEAFPIGHVFDSVTIKGKSSIFFFIDLMSGEIPGEVHKSRVSKGTDLDMDFKIGSTHKARLLNYSMFDNLYILTLDQVKIDVTYLRANDIPVGHKITCEIEKVSASGITVKLENDFKATVPAFQISDIKLVYPERKFKIGSKVKARILDVRPFNSGASLSATLKRSLVNAEESDIVTSYDDLTPEKRALATVEKILPSGCVVSFFGGVSAFLPNAEISESYVKNASEYVKLGQTVKVRVISVDTKQKKCLVSLRISNEVSQDQMSTLEKLVPGKSVVSAEVVEKDRNTVIVKIEGSDVRGIINIGQLSDAPIDDCRGLLKKLKVGEKLEALVLMIDNRKRGVTLSAKRSLIIDAKSGILPTEFSDISISDTVLHGYVKTVIPSGVFVNFGNNLTGLVIPRFASFKKINDLTTAFAADQSVSCTVVNADENNHRFLLSLLVETANSKEKAENPVDKTVKTLGDYSVGKITQGTIKSIEKTHLLVRLSDNQDGRIDISEIFDSVEKVKDLKNPLADFKIGQKLKKTKVIGYFDLEKAKFIYSKKAKSNLVELTIKPKNLENSELIYPASFKDIKEGDAVLGYILSVNNGYFWYSISPAQKAKMSFVDATDDVEKLEHFSEEFQVGTIVPTKVTHVDIDHFAVSVSGRKNTIRSADEIKVGDVLPSLILNVRDNSVLVSLGENLTAVSMVTDALDDYTLRLSDIFHVGEFHTATVVSTDRKIYVSLRSKHPKDRLINSVEDIKRGDIVRGYINKINNTGLFVDLGRSVYALVRVSDISDSFLKNWKEGFSVNQSVKGRIIEANSENRVLLSLKNSVVNGDLQNLKIFADLKVGDVYEGTIKKVEEYGVFVSLDGTDNVSGLCHRSEITDAPVKNAGDIFNEGEHVKVKVLDINHQKKQLSLGMKASYFKEDENEDEDGDLVMADDSDNDEDEKMEDAPESEDESEDENEVEEKSEGLSESVGLSTGLSTGFDWTASILEQAKEEESSDEEEEFEKKINKKSKKTKAEEAEDKTGDLSTRTPQSISDFERLLVGNPDSSILWIQYMSFQLQLSEIEKAREIAERALKTINFREEQQKLNIWIAMLNLENSFGDEESLDSVFKRSCQYMDAYSMHQKLVAIYIASQKYRKAESLFGTLCKKFGAKHPNAWVSYGSFLIDRDNNEEAHSVLAKALQILPKRDHVDVVRKFAQLEFQKGDPEQGRSLFEGLLSDVPKRVDLWNVYIDQEIKTDDKKKVDGLFERVIEKKLTKKQAKFFFAKWLDFEEQKGDEKSQDYVKAKAAEYAQKLSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDD4</accession>
    <name>A0A1E3NDD4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015254.1">
        <property type="nucleotide sequence ID" value="XM_019162074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDD4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDD4"/>
    <dbReference type="GeneID" id="30178761"/>
    <dbReference type="OrthoDB" id="3980110at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1224"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1243"/>
            <end position="1255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1255" mass="142745" checksum="0388E4F3F41BD6C7" modified="2017-01-18" version="1">MAAKKARHHKTKNKSKTGGNRPTAKETAPGAGTGIGSNQEEIVFGSDGNDMTAGSPQIMGGMDDPFDEIVAKAMNFSYADSINFKGGNFALLKSIRNMVSTMEENKFLNYMEDIRTGKTGIEDIVELLDSFRIERNESPNVLSGNAHGTAPSTTNGNNDSNKGVRINELEPKRLEHEDHEKGEEEEEGEKGQDDDEEEEEQIEEGGYVSSNENESDNNSGEEYSFDLELSNNFFDPYKFHDALFKVLRITIPNAKGTTSGEQHRIKNLFSMLATLCDPYTQPSSTGDHNEMILSADLISDLVISLIYKEYVKLSDGQTMNLPAFDMAYDIALIIEKIMIILNADDETNLIYINNDEEHWERELPKWLPHSALSNIVNIASDFHHMMLLYTIATAGLLIIQKLYRNMGNICLNPFLSMYLQIWKNQTRIIYLGIEIDRRDEDNGFPGYPEVIRYMIKGASAVRSMISLILNDDFDKRLHDLKHESLVNFMRPWGRKFTNGSITRDIRVFVAALLALGSELDSVTELLFNFDPEDRYDEDIKYMFEMELEDMEEENTTSISADGAAAELKDDTVSGAETSQGANEAHENEHYHDHHKENVRRYVGKNGILEIERHDEEVDEYGDGDDAMFTNQISTSSAYDLHPDCDCVFEDFESDFDEEGEYEEINGEDEAKVEENEERLKQVKQFDNVKLLNDFESLSHLSHIDLKDLDMETIQNLHLEAVRNINNGPSAVRATGSKNTEIDSKGRDWRDIPRGDNVMLTANFVNLLKSSTDDSSIFLTPMEKLLSSLQSMTSETLDSQTCEKIIRSIAWVVQYEHESKLMSDEEVKMNNDPNVNADVIYNFLKLDDNFVKMMTHNPSSAFFIIDELLMAEGYRRVLIWFLTHLPLNQWIINYFHDLLIGQRGNPSKEESDSSTRFSFSRQGPLVLSEVEQSMLLHEFLSNAVIYLSRGSSYELEDIMWNQIEKEKTGRREKNNTTPTNNVSSFITNRSNAQKLIKVICLMLKSLEANKVIDPSNAEYRVEIQTLLVQWVGVGFVPEARELFFKYSSTGNVGKELSGDANGKTIVGKAEKENASSTKENILSRLSQSKFSKLMSYLENFSSFDLFIITLNMAQHYRTEKSTCFDFMKDLNSIAHVYETEEELLKSFDDFYLALNEALSTGDEERIIEIMYASKFLTSETNIIHARRAINIFIEGGELTNEAETNQTLQLLRQNQLTDHVENEKIQNALDNPSPPKPTTLLNSKKTKKKPKHGKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDG9</accession>
    <name>A0A1E3NDG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015288.1">
        <property type="nucleotide sequence ID" value="XM_019164869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDG9"/>
    <dbReference type="GeneID" id="30181556"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="106" mass="12495" checksum="093ACF11E260BE56" modified="2017-01-18" version="1">MQTEKIFDLATKKGVSALIITLFEQMKREVLDIANVSGPVNDTCNSKVDDYFYSERRKLLECFESEQPRGEQCRQEMSSSMNWDLDWLSMENCSDLWTLYMEDLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDI6</accession>
    <name>A0A1E3NDI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ferrochelatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74804</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoheme biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015308.1">
        <property type="nucleotide sequence ID" value="XM_019164875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDI6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDI6"/>
    <dbReference type="GeneID" id="30181562"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="UniPathway" id="UPA00252"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000001">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42830" checksum="41179D4928E5403E" modified="2017-01-18" version="1">MLKNFIRRVSTSATPKSQGTAVVFMNMGGPSTVPETRDFLFRLFSDGDLIPLGPFQNSIAKFITWRRTPMIEKYYKTIGGGSPIRKWSEFQAAKACERLDKISPETAPHHPYVAFRYANPLTKETLQKMLADGITRAVAFSQYPQFSYSTTASSINDLYRLSKIHDPEGQINWSIIDRWPQHPCLVKTFANHITDALQKFPENVRDEVVIQFSAHSLPMDIINRGDSYPAEVAATVYAVMQKLNFSNPYRLTWQSQVGPKPWLGAQTEKTCERITNNESVPGVVIVPIAFTSDHIETLHELDIELKEAIDHPDKVVRCESLNGDPIFLEGLADLVKDHLESGKLYSKQLPLDYKLGSVSAKNVFKHPDEFFGDRSHKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDJ1</accession>
    <name>A0A1E3NDJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015297.1">
        <property type="nucleotide sequence ID" value="XM_019164873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDJ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDJ1"/>
    <dbReference type="GeneID" id="30181560"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF12">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="36607" checksum="CCF3A9B6708A9A5A" modified="2017-01-18" version="1">MAGVRKFKIAAIQVGPIARTAKREDVVERLCGLLQEAANKKATLAVFPELTLTTFFPKWLIDSQEEVDSYFEKGDITKGPCKPLFDLAKHNGIGFYLGYAELTEDGHHFNTSILVDNRGEIVHKYRKVHLPGFVEPIADIPFQQLEKRYFEYGNLGFQAVRTNPEWIDATVGMLICNDRRWPEAWRSYALQGMDLMLIGYNSVANAVGLFGDKEDKTLRQYHSDLSVQSNAYFNSCYAVSVGKCGNEEGQNLLAGSLIVDPTGRTVVQSVIEDDDVLVREIDLDETIIGKQKMFDFARHRRPECYIRLTEQKGAIALPPYSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDM8</accession>
    <name>A0A1E3NDM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015335.1">
        <property type="nucleotide sequence ID" value="XM_019164885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDM8"/>
    <dbReference type="GeneID" id="30181572"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="49" mass="5738" checksum="1C5A2C03E6E99E66" modified="2017-01-18" version="1">MCAGNNITYDHLFIEYINDPNHENIGDTNDYGQLFSSIKYINDPNHENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE18</accession>
    <name>A0A1E3NE18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcineurin subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcineurin regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein phosphatase 2B regulatory subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_18295</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Composed of a catalytic subunit (A) and a regulatory subunit (B).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015494.1">
        <property type="nucleotide sequence ID" value="XM_019161936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE18"/>
    <dbReference type="STRING" id="763406.A0A1E3NE18"/>
    <dbReference type="GeneID" id="30178623"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005955">
        <property type="term" value="C:calcineurin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019211">
        <property type="term" value="F:phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022604">
        <property type="term" value="P:regulation of cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="176" mass="19374" checksum="F8CD496741E4E1A3" modified="2017-01-18" version="1">MGNIPSSLLDDLSEGTNFGSEEIDRLAKRFMKLDTDNSGAIDKDEFLAIPGIGQNPLARRVVDIFDENKGGDIDFKEFVTGLSVFSSAGSVDGKLKFLFKVYDIDNDGYISNGELFIVLRLMVADSLTDVQLQELVDRTIMEGDSDGDGKLSFAEFKKIIESTSDITQKLSLDDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE34</accession>
    <name>A0A1E3NE34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_18272</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015469.1">
        <property type="nucleotide sequence ID" value="XM_019161929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE34"/>
    <dbReference type="STRING" id="763406.A0A1E3NE34"/>
    <dbReference type="GeneID" id="30178616"/>
    <dbReference type="OrthoDB" id="282973at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF27">
        <property type="entry name" value="ACL091CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="214"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="693" mass="78677" checksum="450CF517C8212B9A" modified="2017-01-18" version="1">MQSQADVLSAYFCYYVSIVLLVSFSITRKISFRMLAKTVAVALIAAAVSAAVPSGYEHAPNSVFNWSHPVDGDDALVASSLTVLQSIDNSTDVDSCEKCKSRLLYGKAIAISRPDLIPTIFEQWCLNTGNFSATTCATNYHRNTADNSTTGSNFADLLTLIDPLSIDAEYWCAYKAGGQCKAPELPQNLSIAHLWNNTKPDKAYNVLTPGSDIMKILHISDFHIELDYTVGAEANCTTSMCCTPHSKAAKNAVSANQTSIDDLYTALFKGSYYDLDGTYHKGEKITALHNISVPATTFGNYECDAPEVLINSSLHSIADFQISNNISFDFAIFTGDLVDHDEIKYTSYEQCIEAETVIFNDIKNVFDLMPIYPVLGNHDTFPYGQLAPQKSGFQSKFDWNADLMADLWMDYDWIDIDEAQEAKTHYTGYSVNTKSGLKVIAFNSNAWYQKNEYAYINTVEDPDYFGTFEFLVNELLESEAKDQRVWIIFHIPVSQTMLPPQAKLFTELVERFSPYTIAHIFNGHTHRDEFQILYQNNNGTVESKKESELINFTWITQAVTPWVENNPAWRYYEIDTETFQVMDVHNYYTRLNETFTTEAEPVWEYLYSPRDAYPVEWPKTSPLNATYWHRVYQSMLSDVSVLQTYEGNAKRWSPYTSNCDANGVCDWDLCYVSSFTDDEYETCIKHYKYVSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE39</accession>
    <name>A0A1E3NE39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_60520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015497.1">
        <property type="nucleotide sequence ID" value="XM_019163442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE39"/>
    <dbReference type="STRING" id="763406.A0A1E3NE39"/>
    <dbReference type="GeneID" id="30180129"/>
    <dbReference type="OrthoDB" id="6359943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47369">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47369:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="36"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="617" mass="69991" checksum="D52769F58C62BAE5" modified="2017-01-18" version="1">MVHSTNDVSTCPYGCCHTPSISEHLFLNGLVRGDHYDVIIRAFGKKYKLHKLFLDRSPYFRSLFSWSTSMKSHANTIYDLRFEEDGVFSDSTSLSCKQKSFELAISRLYGAVNLKEECKIPYNMIEIGQYLALSDIVCTATDYIVKNMSILNLAENLSFAIACEYGSASQRIIENGKGILCANGWEQGPDAWDGIPTTIAAEVLGEDYFFVPTEWDRCIFIIKLIERRLESSCLEEEIDNVAPLKKVLNEKIHYCHIPPDQLQELEELCDINGENYIKPHVLHSALWQAVQLETLISRAQDSPHLDNVITCPSSPSKNHKWFKVPSKDETLSGLPKELDVLLSQSLSATAVNQLNSDDSKTTQGENNSFDTDEKMFVWTKIPPFRFSISFANVSELSTDKRVYGKTFWYAGSYWNLYLQKSHIQSKNSYQVGVYLHRAHNGSSNSSSKSGLINPDIYANNVNYKLSPKKYQRQEKILSYQTVPIERTEPNSNELYGNRSNESTLESLGMNMIDLSVSDDSKPTVASYDRTTKAMKRKSNGMISYEDQRNAIKVYFIIFTPSRRAMPTITSFLSVPNDFSKSQSWGWKSNNMCVFNEDGTFAEGQDSNLKFMILLGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE80</accession>
    <name>A0A1E3NE80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED6</name>
        <name evidence="10" type="ORF">PICMEDRAFT_60549</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015537.1">
        <property type="nucleotide sequence ID" value="XM_019163443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE80"/>
    <dbReference type="STRING" id="763406.A0A1E3NE80"/>
    <dbReference type="GeneID" id="30180130"/>
    <dbReference type="OrthoDB" id="344220at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="245" mass="27364" checksum="A7B940616E143B96" modified="2017-01-18" version="1">MNDRQEVLDELEWRSPEWINIYGLRSDNILEYFSLSPFWDRQCNNQVLKMQRQFQANDRGSGENGLPPMMIIPSFDAELRRLRGIEYVVHMIKEPDLWVIRKQRRVDDGSSAYAKNSGPAAISATMPLETYLAGGPGLAVPDDVVVLADYFCVGSKVYMASNVHAIVRQGVLSLSRSLSVAADRLGRFTNSTSTVNNIDLRRATTGSADAPPANGADAPMGASAEEKWKREESLFLLAVQKRKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEG4</accession>
    <name>A0A1E3NEG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_37068</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015627.1">
        <property type="nucleotide sequence ID" value="XM_019163053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NEG4"/>
    <dbReference type="GeneID" id="30179740"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="10">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="286" mass="30760" checksum="0D132F84F5327352" modified="2017-01-18" version="1" fragment="single">VVVYWGQDSVYQVDQTLEKDLDAYCTSVDADIFIISSVNQFGAGQETTINLANHCGSTTSCPDIGRQIVNCQNAGKKVFISLGGEDRKKYSLSSSEADSFATELWNKFGGGSASDVVRPFGDAVVDGFDLDIEGGDNSGYPALVQNLKSLFSQDSSKSYAISAAPQCPYPDHKIGNVLSSPGVDYAIVQFYNNPSCELTTTGFNWNDWAGLAEENNFKIFIGLLGDGKSTDNGYTPIDEVKAALQDVVSKKPEPFGGFSVWDVGRGVANIDNGKSYIQELSDLISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEH3</accession>
    <name>A0A1E3NEH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial 15S rRNA processing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_13704</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015632.1">
        <property type="nucleotide sequence ID" value="XM_019160099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEH3"/>
    <dbReference type="GeneID" id="30176786"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF329">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR DMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="724" mass="82177" checksum="5345EED23DBED5BF" modified="2017-01-18" version="1">MLSRSLRTACSNKGYKTFSLAKSYPTKSTSRLLSTGTADAKSVDSKNVLKFNKATNENTKSEVSGQKTKGDFQNKNQFISTLYDSLETDYTEFSPLFEKVQQAFLKASPRDRGRSFFSMIPLGNALLTRILDIKKKTNNDVPTSILFKEIVSLLIENKLIHVSHFNRYIRELIAEKKYLNALTIWIENVNYFKDTPQAFVSKINNSGISKEYNLLGLSLYLLSLIENNEKKVDPEFIKLIFGDKPSCSLSNFQYFVDGLTLPSEDAQTIVSLYSTYTSDLFDINSSESLKGIRVASVDGKVVHLENTINKNLLAYNGREAQIKPSTIAHYMEYSNNCKLYSRSIELWKFASLNKIELTVEIWNQLLSSFASSAIVDSRNKVESVWKLLNESVRPNSQSYSIYVKYLLKNKEIGRVEEIINDLKKNQPKLFDNSLKCAMIEFLLLSDKTTESLQLFKIYQQSETFVPTIEIYNKLLSKLITEKNFDDAQKLLDELMSKKYENISPDIATWTTIIDFLLKTSARSNLTKDEILDKILSIIKTMESHNIKFNSVALITVANNLLRNRQTSDLGFSILQSMESSNVKLNAVGYSGIITAFTSVGDMTNSLYYYDKALKNGIKPTAFLYNSILKGYSRSPNIPETKKFMEGIKALAEANPHNMKLLPNKYTFYYLLSQGVALKDRDFVHDVLKELSETNTELGSSLPTILKSLKESGYNIPESLAQKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEH6</accession>
    <name>A0A1E3NEH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WLM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_60243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015660.1">
        <property type="nucleotide sequence ID" value="XM_019163418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEH6"/>
    <dbReference type="STRING" id="763406.A0A1E3NEH6"/>
    <dbReference type="GeneID" id="30180105"/>
    <dbReference type="OrthoDB" id="49605at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013536">
        <property type="entry name" value="WLM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053000">
        <property type="entry name" value="WSS1-like_metalloprotease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622:SF1">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08325">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51397">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WLM" evidence="2">
        <location>
            <begin position="15"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="247" mass="27833" checksum="0B02E088A1C72963" modified="2017-01-18" version="1">MVVKGRGKFPPKKTPELPSPTPNITKIASLTKQPASDKALELLHEVATLVSPIMHHYNFKVNLLCEMYPRNQGLLGLNVNRGQKICLRLRSPTDSKWFLERDEIVGTMLHELTHNWHGPHDAKFYKVLDELKDKYYEFQVTQSMKNSSYPNNFSASGAKVLKPMSTKKYTTKVSKLGTGKKLGGSAERLPLRSLMLLAAERRLKDSKTCNSDEECDSQLPGDNVKDSQPTTEAKRETVENCDVICID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEH9</accession>
    <name>A0A1E3NEH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Found in mitochondrial proteome protein 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_21420</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015654.1">
        <property type="nucleotide sequence ID" value="XM_019162199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEH9"/>
    <dbReference type="GeneID" id="30178886"/>
    <dbReference type="OrthoDB" id="2968323at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051035">
        <property type="entry name" value="Mito_inheritance_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="566" mass="66340" checksum="00125B0BF4DDB299" modified="2017-01-18" version="1" fragment="single">TNLFNDADPNRNHMFEYSWGTWLKNDEIEKQKRLTKFSIQGLNDLIKRIISIEKSGVVKEKNPDEIKRIENIRVLSNNIAHFFKDSKNENNIKQIVSLHEGKHHRIYRIEIEGVEKKLVLRLPYTLHSQLFTKRKLESEVATMDFLTNAFNLNIPKVLSYSGDYDNFVGHPFILMEYVDDVESSLMKKWNPLMESKDDRLDDPEAIEKLNEVIEPLADFNKIVSDFVFDNYGSIYFKDDCPENLEKVAYENQDRWVIGPTVETAYYRNKQYVKEEDLNKYVGPWKGSEPLKMIKDLVELELHSLRVRLSLVDSGKVTTDTKEGLEFCIKIFEKLDKIAGEMFNLNENETLIPNLNELLKPRLFIGDLDPMNVLVRSGEKGYEFVDLENSVVKPFLISSYPKFLEYNGAKIFNLKDEIENFEALDEIEQEQYKFMFKRTRNQFLWEVSINKRSKDLVGVISPVIKLIKNCYLNVLNFKQPKDSLYIENGLIEISTMWDNYNENGVVGKFKGEKNPIKFTDDEIKEFTDEIRDYEAENSSNPFIATDGWVPQDMFKRLQDQGMIVRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEI5</accession>
    <name>A0A1E3NEI5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015642.1">
        <property type="nucleotide sequence ID" value="XM_019161992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEI5"/>
    <dbReference type="GeneID" id="30178679"/>
    <dbReference type="OrthoDB" id="4088270at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="55" mass="5809" checksum="B31829E623593469" modified="2017-01-18" version="1">MGKTDRTISRLVAAARGKKEGIAAPEFRVWSPTTLLGQALSSLTTVDRTGNGAFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NET0</accession>
    <name>A0A1E3NET0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_79272</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="14">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion inner membrane</location>
            <topology evidence="15">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015719.1">
        <property type="nucleotide sequence ID" value="XM_019164968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NET0"/>
    <dbReference type="STRING" id="763406.A0A1E3NET0"/>
    <dbReference type="GeneID" id="30181655"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06502">
        <property type="entry name" value="TA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="16">
        <location>
            <begin position="96"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ44606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="468" mass="51611" checksum="18406A10FAD5E93D" modified="2017-01-18" version="1">MSAVAIEQSNKPQPSATIVDSAWDTALSNAIVKTGLGFGAGVVVSVLLFKRRAFPVWLGVGFGLGRSYAEADSVFRQAGLRKKMTNTEPLYATHNEFRSDTFTVPTRKMLESVLTCTTGDSVYKEDQDCLDLEDKVARLCGKEAALFCSSGTMSNQIGLRANLFQPPHSILCDYRAHVYTHEAGGLATLSQAMVTPVIPANGDYLTLDDILDNYIPDDGDIHMAPTKVISLENTLHGIITPYEEIKKISAWCQENDIKFHCDGARLWNAAAETGISMFDYCTLFDSVSLCLSKSIGAPYGSILVGSRKFIEKCNHFKKQNGGGIRQAGMLARMASIAIDDNFSKLQISHSYAKDFGRFCQEQGIPLESPVDSSFVFLDYKKVKINPHVYELASKKYNVKTMGNRVAFHYQNSPESIENLKLALLEAYKDSLVNPYYTKGASKLYRSSSSGSDHTLVEMNAIAERKARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEW7</accession>
    <name>A0A1E3NEW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Micro-fibrillar-associated protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_174989</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015783.1">
        <property type="nucleotide sequence ID" value="XM_019161625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEW7"/>
    <dbReference type="GeneID" id="30178312"/>
    <dbReference type="OrthoDB" id="1111734at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="130" mass="14469" checksum="BB419870FE87E41A" modified="2017-01-18" version="1">MVSSGSDSDSDSGSGSSGSDEPTFHKKVAFRKTTPQQQQKDKADAPAGVAPTLEDVAVSSINRNLKRLEREQLHRAFQDSLVLDERVLCEAVDDADVPDDAVELLAWKRRELDRLHRDRNRRIAREEGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEX1</accession>
    <name>A0A1E3NEX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74382</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015782.1">
        <property type="nucleotide sequence ID" value="XM_019164754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NEX1"/>
    <dbReference type="GeneID" id="30181441"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF35">
        <property type="entry name" value="RE23632P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="113"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="331" mass="38225" checksum="D3E5EE1187F02D8E" modified="2017-01-18" version="1">MPFNLKKLCVDRNNLGLTRVPFYKTLQTWKLTDALYVLLIFPLDWYYFEKIEPYQRQFAVNDITLQHPFAEHERVPGDVLIRWIMVIPATVMLVLSMVLTPRPFKLYVAYVSVLGVFISMGTCIFVTDVFKNWIGRCRPDFLARCIPHESALPDVLYFAKDICTTTDKGKLLDGFRTSPSGHSSMSWTAFGYLSLWLNGQLNCSHIHRGAWRSIIAFLPCLLAFYVALSRTQDYRHHFIDIILGSLLGAFIAWWSYRRYFPELKTRACYIPYVLLERIEPAESLSDIGDELLVGHDYYKDIQLAMGTYVPTGTDPIENDLESNNHSSFSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF18</accession>
    <name>A0A1E3NF18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="8">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18131</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="8"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015850.1">
        <property type="nucleotide sequence ID" value="XM_019161889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF18"/>
    <dbReference type="STRING" id="763406.A0A1E3NF18"/>
    <dbReference type="GeneID" id="30178576"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF12">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYROSINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="9">
        <location>
            <begin position="56"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="364" mass="39344" checksum="046652F92253894B" modified="2017-01-18" version="1">MADSRSNSAAPAQEEYDDLRILASDPLISPELLIREIPATKDALKTVIRGRKEATAIVKQQDDRLLVVVGPCSLHDQKTAMDYANLLKPLAEELSGELVVIMRAYLEKPRTTVGWKGLINDPDLNGSFNINKGLRFARQLFVDLTNLGLPIGSEMLDTTSPQYLNDCLSFGAIGARTTESQLHRELASGLSFPIGFKNGTDGTLGVAIDAIKAAENSHHFMGVTKNGLAAIITTKGNDSCFVILRGGKAGTNYDEKSVQEAKEKLPAGKVLMVDCSHGNSNKDYRNQPKVCSEVARQVTNGENAIIGVMIESNINEGNQKIPGDLSQLKYGVSVTDACVNFASTEIMLKELAAAVKARRALNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF24</accession>
    <name>A0A1E3NF24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">U3 small nucleolar RNA-associated protein 25</fullName>
            <shortName evidence="9">U3 snoRNA-associated protein 25</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_36543</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
            <location evidence="2 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the UTP25 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015837.1">
        <property type="nucleotide sequence ID" value="XM_019163015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF24"/>
    <dbReference type="STRING" id="763406.A0A1E3NF24"/>
    <dbReference type="GeneID" id="30179702"/>
    <dbReference type="OrthoDB" id="10264378at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 9" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="12">
        <location>
            <begin position="279"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 C-terminal" evidence="11">
        <location>
            <begin position="584"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="107"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="771" mass="90631" checksum="1ED0BA02D993AEB3" modified="2017-01-18" version="1">MTTSGDQQGPASGPKRQKKSKFGRSELRSVTRTSRRNDSREEKLDNDDNADRGQYGDVASDDDIDDDFDKDVRKTTIASKRINRKENKMAYSALISLLKSEHQIDELKDGQGDEPEESASEEDDQNTDSGSDEERHREVEDFLDEEEKLQDNIDVHNKYDSFNLHFNQEKEISEAIDKYESLPVKDRRIKLISKEEIADDEEKYVRYRYGYPQANPELAEKRKTGTMLEQKLKYHNVKKRIQEKFNNFSDIRRLDTELIESMLTYETFNFQYLNNERIKGKYQDYYLLHAMNHLMKTRDRILNNNEKKTLYEKRIKEGSNDEENDDGIEPEYRDQGYTRPKILILLPSRNFAYTVVSKLIDNLQMENVENKKKFKTQFYDSSKISDRVYHSRPKDFEEYFDGNTSDLFVLGIKYTRKTLKLYSPLEQSDIIVASPIGLLKLYNKIESQAPKKKVDKNDKNNEGMKNNFLSSIEMCILDKAEGMMMQNWSNVTDILKKHLNQTPKEFINVDFSRIRMWAINEQSQYLTQFMCFDKYTTPEMISLVKGSKNVLSGSTSYIPLEQMSVIDSTNYKLYQLGLVNKFERLKQTFMRFDFDESSIANEPEKRFEFFKNVVLPQMNKSSYKYGTLVYIPSYFDYLRVKKYLNDESSISFVAIDEYSSNSRLTRNRALFKNKANTATLMLYTERLHFYKRFDLKGVRNVIFYQLPSDPDFYQQVLEFVANEKLQDDLDLNLCMVRSIYSKLDAMRFGPIVGTNNVARLARGANELTEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF30</accession>
    <name>A0A1E3NF30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pre-mRNA-splicing factor SYF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_74402</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">May be part of a spliceosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SYF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015809.1">
        <property type="nucleotide sequence ID" value="XM_019164760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF30"/>
    <dbReference type="STRING" id="763406.A0A1E3NF30"/>
    <dbReference type="GeneID" id="30181447"/>
    <dbReference type="OrthoDB" id="199717at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="63"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="235" mass="27705" checksum="BBBF33B1283A99DD" modified="2017-01-18" version="1">MSSDTRSRLKRGVDADVDDTSKKSTVQDRIRELKNARAKSVLENRRAVYDEVAGVKMKLAEKRKLEKSEDDAVLQEEEGRVSKEKKKNSPNVSTLEYTAEEDEKWEAKQGRINTHEELNDEHKGELHNYKQLAERTYSKNIRGIEQQGKKTLEKYQQEKSLYEKLKTQGLSPEEIRTRLTSSGQLDTYVKDLKSWEEEVYRKRRKINDEGKDGAIHEKNRQFNSKLQRHYRQFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFD9</accession>
    <name>A0A1E3NFD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arginine N-methyltransferase 2</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_12608</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that methylates the delta-nitrogen atom of arginine residues to form N5-methylarginine (type IV) in target proteins. Monomethylates ribosomal protein L12.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015955.1">
        <property type="nucleotide sequence ID" value="XM_019159951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFD9"/>
    <dbReference type="STRING" id="763406.A0A1E3NFD9"/>
    <dbReference type="GeneID" id="30176638"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="7">
        <location>
            <begin position="192"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="416" mass="47537" checksum="5CB1D61DE738976B" modified="2017-01-18" version="1">MSDLHELCYFKNRPITREEYLVPLKAYLKAGIPATYTTEEAYNYEHGVEKEAESNTTPMHLICEHVPKDLNEEEESVVLEMMEQLWLNGAGWCLLNDKQETPACVLLRRGLHGSKYWDSCVDSGVRAEVLLRKLNDPNVEFLDDPADEFSEVAEEDDVPELVDETKLSTEQNKESGEAAKAVEIVQEMYNGPAGATDTYLNTELEYKNGALVTKEGADGVMMQWEDELMKAGSESLFKSIEDPNDINILNIGFGMGIIDTMIQEKRPTKHYICEAHPDVLRKMKEEGWYEKPGVVVLEDLLTKGVFFDGIYYDTFSEHYEDMVNDLFDVVVGLLKPTGVFSFFNGLGADRLVCYEVYKKVVDLDLSNYGMSVEFKTMKAPVTTLQENDDSPDSVWKDIKRAYWRCPLYYHPEVRFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFL4</accession>
    <name>A0A1E3NFL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73736</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016048.1">
        <property type="nucleotide sequence ID" value="XM_019164568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFL4"/>
    <dbReference type="GeneID" id="30181255"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF319">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="229"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="421" mass="45918" checksum="18EA969992C6C94B" modified="2017-01-18" version="1">MTDIEGKKIQLNEKEHNIDGGLKAWLVVGAAASIMSMSFGMCNSYGVYQTYYEEKYQHVPSNVLSIIGALQAGLTLFMALPSTIAMIYVGPQLVVAIGGLLACLSFMFLSISKTVAEIFIIQGLMFGLGSGIMYVHATGVTFQYFNKRKALAQGVITAGASLGGVYWPIGIRNLIDKVGFGWANRIIGFIYIPMVIIAVVFLRPRVKPKPKLDNENFLRLNFTVLLNLRYLMVSVAWFLFLLALFPGFFYLDLFCNRAKVDPTFQQYTVAIINACACIFRVIPGFVGDKCGRMNLLIPALLLSGIFPLAMWIPASNSAVIMAFVVLWSCATGVPIALFPPIIGQLFGGKDIPSYLSFFFVFGGLGNFLGPIIGGTFIPKGNVSNTEGFDKLAIFCGVISLGSAVLVIIVRIADTKKAFVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFM4</accession>
    <name>A0A1E3NFM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016058.1">
        <property type="nucleotide sequence ID" value="XM_019161607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFM4"/>
    <dbReference type="GeneID" id="30178294"/>
    <dbReference type="OrthoDB" id="3998114at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="443" mass="50573" checksum="A9FBA740FBCE1355" modified="2017-01-18" version="1">MEAAKTDTSEEQLEYESDEDEYELLYVESQAGGNENLNKDHVEDNIGNETEYFEIDTQEFNTNTGEKYVVTEGYPEIEVEDNFEEDDYEDEVQDSIDSDDVVNGKTESYGSERDSSIEYQGEGVGGFKDVVDGQSDGNTHFVVEISSDEDEDEFNNHGEGETQGEQETKKARETESDGVIEETTKEAADDPFDDDDLIEYVEDPDEVYERPEISVAGSKQQNQDEEDKETRERVGGDNTEIRTLTHLPIYLDFCDLVSDKEKDLGNAWLNEEIKVDFDFMKLYPPECEEDEMYAEFDSLYGSLEECIRLKFIDILLKIKSVITKSCKIQDLETDIDIRLTFGDLMNFTVASDSLSSESLTLEDVLISYERLKLQSPNADLHKFLLLRVWLSRNTLGQFGILKEASLNGYRLENLSSLISRKRKGEQDDMNMVATKRNKVDTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFS3</accession>
    <name>A0A1E3NFS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18327</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015531.1">
        <property type="nucleotide sequence ID" value="XM_019161954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFS3"/>
    <dbReference type="GeneID" id="30178641"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF189">
        <property type="entry name" value="CYTOCHROME C MITOCHONDRIAL IMPORT FACTOR CYC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="66205" checksum="F7F97D0EB95BFF5B" modified="2017-01-18" version="1">MQNLKLFLRPQLVAPHLRGSPLNFPKALRYKSSDSDSETKNSNESNKAGPSENNITDNNLPINRTEGAHDLTEKFNPDLSSKVTKPSSALPAMDPSLRTLMAKTKVEYNPVTNSQQQSYEFKAPKTPKQLYKEKMAEKARPKSKLRKLLPSLIICGGLCWGIFTYKYMTKDSNPNVDTDSALLREDKFLPYLISFKHKINDDHYLIELTRKNRAEKLIHNEQLFNGDRLWSVEIMQPDINIVRNYTPLPMYVAGIDPNTKEPHLRLVKELAEEGKFILIVKRYNEGEFSRWLTGRNLLEEINVRGPIVEYKMPYHPLNRYDERPQMSNTLSSIKPDPIWPENVPKPENYVFYGAGTGILPLFQLIYSPNPPRGFTDVFYSLRDESELLPQIKTLNFFAEKCGRVKFHYLISNDKKPNHLTAEKVSSPTLPNFTGGWDLRMSEEVYKQKLLREKKAEVEKQMERASNSLKSLTGNATLVSKDAVIVTSDSNSSSSASSTYIQPTNSRIKPESAYQQWVFFKRAKGDSTTPPPSFAFVCGPESYISDVSGKPDLNNLEKKDNGPIGGLLMEKGWNLNNIKRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG31</accession>
    <name>A0A1E3NG31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016192.1">
        <property type="nucleotide sequence ID" value="XM_019161651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG31"/>
    <dbReference type="STRING" id="763406.A0A1E3NG31"/>
    <dbReference type="GeneID" id="30178338"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00027">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053036">
        <property type="entry name" value="CellCycle_DNARepair_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031906">
        <property type="entry name" value="RTT107_BRCT_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667:SF1">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16771">
        <property type="entry name" value="RTT107_BRCT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="114"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="307"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="852" mass="96292" checksum="86DF051F58A4E441" modified="2017-01-18" version="1">MAMGTTLGDVQLLAVEGTAQNGMNSAALEELKIGLDSIESLNSDDAMKKFNYKQLEKMSRYELSQFTHVVSNVIDFPKYDVIVDSLMIPVVTTQWVKDVIKNKRVIPLRSYSPDPKHFFKDVILAAGGGLQDDDKEVLKIGVKTFGGIYLDDLKKSLTHYVTNDLEDDNTKLIMAFNEYNPSGKIVKIVKVDWIFHSIMQSSVLPESLYSFNDGKAEKGKEVVIQKPDFLSGLNFALSDNLNISDTMRSYFTMFDDGDKAGERVFLTRYLNNEIDKSYKQKTFDYLFSLLFHKKNNMPYDSLLFYPVNRNPISGMAETLIATTNYTGDSRIYIEELTMRMGGKFSKTMKSTNTHLVASKSVGKKFEFAKSWNVKIVNHMWVEDSFKNWKIMDVSEYKNLPRDAKGVRLIGDIEFNGFEYTFEGKNKEQEKVVEHDFETQLQDEKGDIGVLKRKVGNNQEHSASIEVTQSNKKTKMNSIEEKKSSHDPKGLEEVEEKERVLSEPAEAKTIPANPESRVRTRNRRGNEDRKALQEEASTAVKAVIPEEKNPEGKKSEEPDSVWQIQENGEKPAKSVPTKRKAISVEENDQKPKENKRIATKKTVKSEKPYNITAIVTGFDGSLSVGDKRELRKVGINIVENANKTINCIIAPSLLRTQKFLTALSYSPEYFLEPLFLSDVLGTLDSVKKVGDFDSISPKIEKYDIWEHVDYEKDIMPKRLFRTNTTREKAIEYLKRPRKGLFEGFVFNLSPGLAGGFDTLKGILKAFGCKQCFLFKETTKTAAANVEKNVNGVSDVAILVCSTAEKNVQKHFTTACKNSGMKYVTLEWDVIVTAIFEATLDLNSGNVLQQSGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG41</accession>
    <name>A0A1E3NG41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_35542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016223.1">
        <property type="nucleotide sequence ID" value="XM_019162951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG41"/>
    <dbReference type="GeneID" id="30179638"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF319">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="44883" checksum="E10EB6CB36F2930A" modified="2017-01-18" version="1">MTPPEGGFTAWMVVGAASTIMAMSFGMCNSYGIYQTYYEEKFLNTSSNVLSIIGALQACLTYFFALPSTVIMGYLGPQIVVAVGGLLSCLSFMFLSISNSIWQIFIIQGLMFGMGSGLMYIHSTGTTMQYFDKRKALAVGIITAGSSLAGVYWPIGVRNMIERIGFPWANRVIGFIYIPMTIFSACYIKPRLPPTKRKPEENFLRIDFGVLKDWKFQILNVAWFSFMLSLFPGLFFIDLFCLRVGAPRSLQLYSVAIVNAAALVFRIIPGFLADKFGRINLLIPSLLFSGIFPLALWIPANNTALTASFIVLWASATGVPVAVFPAVLGQLFQNTGHYYSYLTFFYIVGGISSLCGPIIAGSFIPRGHVDTTEGFPRLAIFCGVLCLASCAIMIGFRSLYTLSIREKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG48</accession>
    <name>A0A1E3NG48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_12854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016230.1">
        <property type="nucleotide sequence ID" value="XM_019159976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG48"/>
    <dbReference type="GeneID" id="30176663"/>
    <dbReference type="OrthoDB" id="3995400at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="476" mass="54306" checksum="DE056D7A48EFC8B7" modified="2017-01-18" version="1">MSVTVPPLLKKHMEEKQDHAFADLKSLLAELTLDHKPQSKPKSDSLDSFHSQLIDDVLSKKNSDQQKDNLDSNEKSATIFNYFANITKNKLPQKCLTYFSGEGKTETDNVSLYEDEKSQTKTIYRSNSFDDLEDELVVEGSNKKTEQLDADSIHGFHLEDFSDTFNLENDSDEGDLQDQGCLLTSPQKSEKIKPGLVSKTSSECEKVVNTSSVEIADNFPSIGSNTSFQFDDILTGDILGELDIADIPDSRSGHISSRSSLDLDQARIVNVIVAKLKEQLQPTLERLSQESEKYELMKTVYKLPSVSNLNDEVKQQFNGKGKKYSLDFDKDMVEEMVEKELNKAERRKKQFSQVSMISAQDQVRLNFNKFRQLGTIMEEQELEKSIGRGDESPPKNYICMSNTYSKSEDTRHVHELKTEELVHLLQKIKEGKLKEMSKVKHLVTMFEEEQQDVVNRLDNQKLKNVLLVELNKRKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGR8</accession>
    <name>A0A1E3NGR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">12 kDa heat shock protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_36444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016422.1">
        <property type="nucleotide sequence ID" value="XM_019163007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NGR8"/>
    <dbReference type="GeneID" id="30179694"/>
    <dbReference type="OrthoDB" id="2348401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="12702" checksum="F87E9ABAAEE80C9F" modified="2017-01-18" version="1">MADAGRKDFSSKLSEAVTPDSQKSTYDKAKETATDYLDKAAAKVTPDDQKTFGQSVSDHVQQGHDDAKSKLDADSKTWGDSANELLESGKKTVNEAAEYVSGAVTGAKEGADKGVSEVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHA8</accession>
    <name>A0A1E3NHA8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Palmitoyl-protein thioesterase 1</fullName>
            <ecNumber evidence="1">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Palmitoyl-protein hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_17454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.2.22" evidence="1"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016061.1">
        <property type="nucleotide sequence ID" value="XM_019161608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHA8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHA8"/>
    <dbReference type="GeneID" id="30178295"/>
    <dbReference type="OrthoDB" id="10263094at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF8">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="315" mass="35992" checksum="B49BE77DF1988CBF" modified="2017-01-18" version="1">MEIWGKLLGVLLTIDNASLITGPRDAVVSSIEHVAAPITETSSDRLPVLLWHGMGDSYESASMQWVSDTLQKEHPDLDIYSIYIDRDGGKDKEASILGDAMTQLTDVCEQIQNIEFDPKQGFNAIGFSQGGLFLRALVQTCDVRFHNLISFGSPQNGISDLPPCQPGNIFCERRNALIKSRIYTDYIQNNIIQAQYFRDVNDYGTYLEKSAFLKYVNNELFKDLKYYDRFVSLNKFVMVMFDKDEMLVPKESAWFYDEDQETGDLISFNKTDSYTFDLIGLKSLHDDGKIDFLKIDDLHLKISEKDLIYIAKTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHC7</accession>
    <name>A0A1E3NHC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16839</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016644.1">
        <property type="nucleotide sequence ID" value="XM_019161355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHC7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHC7"/>
    <dbReference type="GeneID" id="30178042"/>
    <dbReference type="OrthoDB" id="426001at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14521">
        <property type="entry name" value="DSP_fungal_SDP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="362"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="454"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="538" mass="59073" checksum="67BA43F08FBB03C2" modified="2017-01-18" version="1">MYHNTACNTSRNHTQTTTTSATALPSSPPTTHTEQPENYLSIRESDTKKMIDLLSPNSSPSLPTRKSKNLKNLQLNLTNSPRNNINNHEIPNTSIPLPNQRTEDSTAPNSSSSLNTLKTPVLSRTPTPTGNLPAVGAGAANGTGAAISPFSNPRPLQHFRRMASFNSSSPSSSSACSSINGNSIKLNLSGGNSNGSTNTINNSNINNNQRKRSATTLAINIPVHDVEAENNGDSPGNGHAPGGGLKSAYGDFIFNYDTDLPNDRNASKTNNKKIPVLPFEKPQKRTSSMDFSSQDSLSLSPISFSSPSISLPAKPFPVPVDLNSSGQLYSSEIKPQTIMHSFDESKMDETNAETFKMAYPNGPICVLKPNLYLYSEPTFDEVTDFDVIINVAQEIKDYTNQVDERNKIAEIAGIKNFDNISIDSQNREKLTFRNSRQNIEYYFIPWTHTSRLTSDFPYLTALIEKSLKSNKKVLIHCQCGVSRSASLIMAYFMKVFGGGYNEAYGRLKQIVPQISPNLSLIYELIEWGEWLEKGQSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHS6</accession>
    <name>A0A1E3NHS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="2">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_73192</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016792.1">
        <property type="nucleotide sequence ID" value="XM_019164409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHS6"/>
    <dbReference type="STRING" id="763406.A0A1E3NHS6"/>
    <dbReference type="GeneID" id="30181096"/>
    <dbReference type="OrthoDB" id="427800at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000002">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycosyl transferase family 3 N-terminal" evidence="11">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="10">
        <location>
            <begin position="85"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="361" mass="38835" checksum="FD513B8C5363A70F" modified="2017-01-18" version="1">MSKPLTPYLRKLVATPPELSLADFAAALDLIFAGDANDIEIASFLTGLRIHKLDFDARYIAQAVETILKYSEKIPDELVDPLGYLDIVGTGGDSQNTFNVSTSAAIVGAGMGLKVCKHGGKASTSASGSGDLMNKLGVQLMKVNAESTPLIVKNSNLCFLFAPAFHHGMAKVVSVRKQLGIPTIFNILGPLLNPINIRARILGVYTETLGRTYCEAAVRIDRARGKMANTMVVWGEVGLDEISPVGKSKVWFYSKDADAIHEFELTPADFGLPEHSLDLVRSQTPAENAIVLQRILDSDAAALRPGVDPLVDYILMNSAALAVVAGLTKDWKEGVEIARKSIFSGAAKKTLQDFIDAVNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHT8</accession>
    <name>A0A1E3NHT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16990</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016815.1">
        <property type="nucleotide sequence ID" value="XM_019161417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHT8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHT8"/>
    <dbReference type="GeneID" id="30178104"/>
    <dbReference type="OrthoDB" id="2996783at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000001">
        <property type="entry name" value="1-Cys peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF9">
        <property type="entry name" value="PEROXIREDOXIN PRX1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="30"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="7">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="243" mass="26546" checksum="C0C538273DE8D17B" modified="2017-01-18" version="1">MFSRSILRNSAFKASAKRFVSFDAASQPRIRIGSAAPNFKAPTTVGEIDFHQYLGNSWGVLFSHPADFTPVCTTELGAFAALKPEFDARNVKLIGLSAEGIPSHKEWIKDIEEVALGGKKFTFPIIGDESKEVAYKYDMVDENGFKALQSGGPIATIRSVFVIDPKKTIRLVLTYPASVGRNTSEVLRVIDSLQKGDAKGVVTPVNWQAGEDVIIPPSVSNEAAKEKFGSFSEVKPYLRFTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI23</accession>
    <name>A0A1E3NI23_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016331.1">
        <property type="nucleotide sequence ID" value="XM_019161705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI23"/>
    <dbReference type="GeneID" id="30178392"/>
    <dbReference type="OrthoDB" id="3996094at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="783"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="828"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="842"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1040" mass="118236" checksum="56FC4D4FEF281CDF" modified="2017-01-18" version="1">MKGSLEPDRLVGVYSTRLDEKLEDMLDEFDNFDYTHKPLLTQAEFEEEFLVIIEKLFRRRNKYQMVSSIFEKIISKSLNDQHKFLCLIDFMVNISRYDPANFKNLPNDIVANVAHTPIFAKTQELIYNMGRMNYQFKKFSKQAAKESKSDAKDETIAVLPQQNTVPHALDTNDESGSDSADEPANRIMDVSLAELTKKIMSKTKTATKKMNEKKSGLAQKEELNGNEDDMLDASHLEASDSSDYTRPHSRENDLNQADDYSSDENDEPSSLHTIRERGNKFSSKSKLETLVESDSSDYYTGEESFDENEALVDDKNVDTMTVRRVRSHKLLVPKGKHVSAKGEETDNKRAAGSNEVEDEDFDVTMNNSTSIMEHLMDYKDSTNDAQRVRITKSRKGILVSRISKGNELMRPDKPKSRSAAVSFGLTKRQKLGEYGNQVHFQKKTLKNFKQISPVKRKAFMVLEKDLDFLYSGLERLKPSCSATLQKEAIKGFSYKPAQYSLFKDLREEVSGVDSEVAEFADSLLNAFVSQLAKRCGGNDIDESQFVHPFEYNYIMEKFEELKQKSVKLPPIIFHKRFDSLFDYEGNVNSDIKTLKIHNTKVKGLMRLTVFETEDQYYQVFKKIVPRDKIGSRIKFLNNLITDGKSLFCFDLVDTVQYMMNDPRYIVDSEEEEEKLIAQQKAAAEGAKKMRINFIKSSSKHIETDKESTETVIVENNDLNGDMSEVVPAKVRSPNANDQAYERATIDSGDHGAKCEIQDNMNDKLIERNIDQEQDSLNKDAPEIERKTGEHEGKSLPQSPLSKKKETQTEGFSGNFEKQEGFVEALEDKSEKLVQNSASGSSEKSEEAIEKKVAVEDEKSEKISQTAEELSKPTYGGLVEKTSNDSTNFAFGEPLTQLELEQKLREKRAQIHLSDKPTEESLNDDRSTAYSSSKGLSISTRAIKKVLSTKECFELLAVLLFPRLKIDPTYEGIPLSSLRAIGSAYDPFRPYLLSLFLSDVFEFLRLPRYFKVDAMMFELQLQDLLKSANPGSKMKAVAFHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIC8</accession>
    <name>A0A1E3NIC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_13049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016441.1">
        <property type="nucleotide sequence ID" value="XM_019160005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIC8"/>
    <dbReference type="GeneID" id="30176692"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="208" mass="23879" checksum="BE98C78609414728" modified="2017-01-18" version="1">MMHAQLEKSTDGNSNEIHNADILSKEEKSLLFKSLLDSVFTKNSNSDATTRSRSTVDVSQLNIDEEKVLIITSWFNNGQKQQTLLQQQTALPRAVFSTYLARDYTKTLWEVFRLLEKRCLKKYLYQLRPEISFYICVQPNDVSNTLPVSSLDIDVLSCSTCGCFSALEQCCCHLLLLYLHLRHHIAVQEVVLWSESDWLLLQGRSAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIC9</accession>
    <name>A0A1E3NIC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial fusion and transport protein UGO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17597</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016216.1">
        <property type="nucleotide sequence ID" value="XM_019161662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIC9"/>
    <dbReference type="STRING" id="763406.A0A1E3NIC9"/>
    <dbReference type="GeneID" id="30178349"/>
    <dbReference type="OrthoDB" id="77989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="59647" checksum="B28A3C00E62DAAE3" modified="2017-01-18" version="1">MNTQNYMEGSLRPYVSRSDFDLHYPIAYQPGIGIINSSTSQSLSQSLPLVQMQRGGSSSTVGATGFADSRVGIHGFGSRVKAPANAPILSKELYGKSVSDLEWNEFFDISNLKSVLLTLTKRFIRGWFKCLISQPFDVVRILLQVGSFRKNDYPTIKPIRQINKIITEEAVDDTEPLSSESDSDVEGRDAYFVNESPSKLRDSRIERLPSRRASGNSKQVRLENERSKRVNVVIEPESLNTFDMISSLLAKEGPRGILKAVNTTFLMNTVQYTIESWITGFISGIVGIPDPLFVDMIHSPNANWSLILSIFSNVFASLLLTQLSLIRTRFIVTTSSRGCRSFKEIIWNIPRFHIFNVPKSLLIPSIVTHFIKSFTTHYPEYLLTTMKINKYNNPYVFNMFSVILKIIGLFMRLPFETLFARAQVNYLLTNKDELPEAMRIDKEDMCIEFGGYYGYLSTLYYIIAGSRPLTYEGQSLEVDVDDLEENRGIKAIFRGWKVGLLRLVSSYTLNLLRDDRYNIVEEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NID1</accession>
    <name>A0A1E3NID1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016994.1">
        <property type="nucleotide sequence ID" value="XM_019164460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NID1"/>
    <dbReference type="STRING" id="763406.A0A1E3NID1"/>
    <dbReference type="GeneID" id="30181147"/>
    <dbReference type="OrthoDB" id="10252633at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="97"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="191" mass="21577" checksum="3CCB72EBBBC9BC2C" modified="2017-01-18" version="1">MKYVQSVESISIPDDVTVNIKARIVKVTGPRGELTKALKHIDVAFEKVNNKLIKIYVHNGDRKHVASLRTVKSLITNLIKGVTVGYKYKMRFVYAHFPINVNIVEEKGAKVIEIRNFLGDKKVRMIPIPEGVTAEVSKVQKDELVVTGNSVEDVSQCCADVQQICRVRNKDIRKFLDGIYVSERGHVVEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NII8</accession>
    <name>A0A1E3NII8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_171878</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016511.1">
        <property type="nucleotide sequence ID" value="XM_019161499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NII8"/>
    <dbReference type="STRING" id="763406.A0A1E3NII8"/>
    <dbReference type="GeneID" id="30178186"/>
    <dbReference type="OrthoDB" id="10264544at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24162">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013881">
        <property type="entry name" value="Pre-mRNA_splic_Prp3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027104">
        <property type="entry name" value="Prp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212">
        <property type="entry name" value="U4/U6-ASSOCIATED RNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08572">
        <property type="entry name" value="PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 3" evidence="8">
        <location>
            <begin position="94"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Small nuclear ribonucleoprotein Prp3 C-terminal" evidence="7">
        <location>
            <begin position="341"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="297"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="486" mass="57119" checksum="C2F37D2659966579" modified="2017-01-18" version="1">MKRGRNAKLGPNVEVKKRARIGVTEHENVKEAKDEEEILKNIKIHPLLQMPLGTQKMGQDDNDISDKKRSSKTVPKIKNPLLSDWKQRDGFTINPYIDQSDLSIVPERKSRALRINEPGKQIERANNLRQRAKQERLELEHQEELKKKNLVPDERIGEDRYGDEFAEPPPYIEWWDVPFLKNGMRRGCRYEGCGEEVVTYKDENSEDNPITSYIQHPVPIEAPWERLIPTPKPLFLTKQEQKRLRKNRRMLLMKEKQDRIKLGLDAAPKPKVKLKNLMNVLTNETIRNPTEVEMRVRQEIQERADEHERMNAERKLNKEERRHKVEQKVEQDRSRGIYSCVFVVQRLVNPKHLYKVDMNAKQLMLTGACLRLRRGRSLVIVEGGLRGVEKYKSLMMRRINWTANESPRTKDGDSPTTAPGPGPALLLEDLSDNRCDRVWEGEVPEARFHKWTEYTFESDEAIIEFLSKYKLENYWRQSVYLEQEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIL6</accession>
    <name>A0A1E3NIL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_27796</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017029.1">
        <property type="nucleotide sequence ID" value="XM_019162515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIL6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIL6"/>
    <dbReference type="GeneID" id="30179202"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF66">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN OXA1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="11">
        <location>
            <begin position="67"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="335" mass="37664" checksum="A32677DB47B60000" modified="2017-01-18" version="1" fragment="single">DSEEREIETSLGFDEDLAAKQELLHNTTLTSDQLGYLKSVGLVDGWWPSDIIQQVLEYVHVFSGLPWWSTIAVTTVAFRVLLFPLFMKSSDTMARSQSIMPETKRLRKEMTNAMTTGDRALQQRKQMELSMLNKKHGVKYSRMFLSPATQMVYSVGSFFGIREMGSLPVEGLSTQGTLWFENLAAPDPYIGLQLISAALYSVAFKYGGDTGNAQFGGAMKKVFMVLPFASIAFTWNMSAAVMVYFGANGLCSIIQSQLLRNAAFRRTVGMFPLPTQAQLAQNPQKGMFDNFNDTWKEMQQNNKVRAEREEEAMKGVELARKQATSNRVIVKSKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIP8</accession>
    <name>A0A1E3NIP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Outer spore wall protein RRT8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017135.1">
        <property type="nucleotide sequence ID" value="XM_019161527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIP8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIP8"/>
    <dbReference type="GeneID" id="30178214"/>
    <dbReference type="OrthoDB" id="10012223at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30911" checksum="E8390BC4120A3F31" modified="2017-01-18" version="1">MSSRPLYVDWQQAVQARAFLEVARPFNVVYQLVTAQGYLYPFRGFVFFWQNYQPLLPLFLSVVIPYGILHLYVYTVLLWAILPLNLVIHTLTLGPVGVQVAILASFQQCSCVNNYLFKNYLIAGKLNKVFDTTLCLVGLDRVVIPGKLKRLVPQTLGAQIMEINPINIALSTMSLLYSIVVSLIPIIGTVIFEYNNAIKTAEFSQQRMWRLTRLRPRQMKYQVKENEGVYLTFGFVCQLLESIPVLGLLFCFTNQVGAALMAADIYKNRPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIU6</accession>
    <name>A0A1E3NIU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 4C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_59630</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017177.1">
        <property type="nucleotide sequence ID" value="XM_019163378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIU6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIU6"/>
    <dbReference type="GeneID" id="30180065"/>
    <dbReference type="OrthoDB" id="274995at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001677">
        <property type="term" value="P:formation of translation initiation ternary complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="9">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="122"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="151" mass="17008" checksum="60B5ABB4F62D9035" modified="2017-01-18" version="1">MANKGKGKGGKNRRRGKNENTTKRELILRDEDQEYAQITKMLGNGRVQATCFDGNVRIAHIRGKLRKKVWMSQGDIILVALRDFQQEQCDVVHKYNADEARQLVSLGELPESAKINQTDTFGGDEDEDMNFEFGAESDDDGEASDLDIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIV6</accession>
    <name>A0A1E3NIV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_73548</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017185.1">
        <property type="nucleotide sequence ID" value="XM_019164510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIV6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIV6"/>
    <dbReference type="GeneID" id="30181197"/>
    <dbReference type="OrthoDB" id="421393at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07322">
        <property type="entry name" value="PriL_PriS_Eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016558">
        <property type="entry name" value="DNA_primase_lsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009449">
        <property type="entry name" value="DNA_primase_large_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="11">
        <location>
            <begin position="325"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR009449-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="520" mass="60224" checksum="F4118581DF719456" modified="2017-01-18" version="1">MFKKIKRRTQGRRNFDGPVTYLSGPADETILKEYPDRISFYKLPPMTEVTLDQFETYAIDRLKILIEISNLQSQGTNYSDLIKLMTERIKMLMPLHSLNTLEPDLLLEERRKDHYSHFILRLAFCRSEELRKKFISTETFLFKLRYSQLTKSEKQQFINSLDVPWEEVTDSEKDELKAELIATHFNDVSIGFKQGILGGSSNYIQRKVVDEKDVWECIKDEKVSKLPWENVPDLVASRKVVVRKGDAYVPEFLQMNLLANEFGKYLEEQLLITMRLLPGLDEDERLVPVLDNLSKGYISSEFQGFQDGGEQADSEVNAGNVKQFEKYFPACARRLADGLDEHHHLRYTGRQQFTLFLKGIGMGPNESLKFWQRMFTSGPGAMTVEKFNKEYKYNFRHSYGLEGARINYKPWSCTQILSKPKPNKQESHGCPYRDLHSESLTTLLRKMGMKDDGNLQKVLELSDSREYQAACTKVFEVLNSATIQENIGKGLSVMEAPITHPNDYFNRGVLYEKRLEEKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ25</accession>
    <name>A0A1E3NJ25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI mannosyltransferase 1</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16885</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGM family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016691.1">
        <property type="nucleotide sequence ID" value="XM_019161382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ25"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ25"/>
    <dbReference type="GeneID" id="30178069"/>
    <dbReference type="OrthoDB" id="1741594at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180041">
        <property type="term" value="F:dol-P-Man:GlcN-acyl-PI alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="326"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="439" mass="50958" checksum="13440205F3895D0C" modified="2017-01-18" version="1">MTPAMVKGNEDNKPGCFTSQLARVPTSAILFVSLVLRISFFLFGLYQDENMPLPYTDIDYYVFTDAAKFVAINESPFLRATYRYTPLLAWILVPTTYSTHRLWFSFGKFIFIVCDLVTGYVSMISLPSHYTYLSTIWLFNPMVITISTRGSSESLLTSFVLLTAYFLIRNNGKKLTDIIVSGFLLGVCVHLKIYPFIYAPAFLLYLDANQPLYAPFTQKRLLFALSALASFSGLTYWMYYVYGWEYLDEAYFYHLIRLDHRHNFSVYNISLYLNSATTAEVGSFQFEKWAFLPQLALTLFIIPLSLKEGLQSQDKNFRDSILYKIMFIQTFVFIMLNKVCTSQYFIWFLCLLPQTLVGTTISGRKGLVVLLGWVLTQGLWLFNGWRLEFCGKADIFISGLFFSSSLFFLWNTYMACVFIQDVQQQIRLQRRDLEHRKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ41</accession>
    <name>A0A1E3NJ41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="15">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_17362</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Complex I is composed of about 40 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion inner membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
            <orientation evidence="15">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="15"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017277.1">
        <property type="nucleotide sequence ID" value="XM_019161567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ41"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ41"/>
    <dbReference type="GeneID" id="30178254"/>
    <dbReference type="OrthoDB" id="42889at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 15" id="KW-0288">FMN</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 15" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="16">
        <location>
            <begin position="376"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="505" mass="55869" checksum="F557EC60F16DB7D9" modified="2017-01-18" version="1">MLSFVSRTSSAAVRQIRPSQRLFATLDSTPNRVHGGLQDKDRIFQNLYLQNSHKHGTEPWDLKTAQKIGDWYKTKEILLKGDKWIIDEIKKSGLRGRGGAGFPSGLKWSFMNPPNWELNVGPRYVAVNADEGEPGTCKDREIIRKEPHKLVEGCLVAGRAMNATAAYIYIRGEFYQEAVILQHAIDEAYKHGKLGKNACGSGYDFDVYIHRGMGAYICGEETALIESMEGKAGKPRMKPPFPAGVGLFGRPTTVTNVETVAVAPTILRRGGDWFNSFGRERNSGTKLFCVSGQVNEPCTFEEEMSVPLKEVLEKHCGGVIGGWDNLLAVIPGGSSTPILNEETCQDQLMDYDALRNVGSGLGTAAVIVMNKQADVIRGIQRLLAFYKHETCGQCTPCREGSTYMSKMMDRLAAGQANEKEIDQALELTYKVCGHSICAMGEAFSWPYQGLVKNFKPLMLERIKQYKTENGLLDGGLNNGGWYETAEIKNGEVIKNPMPKEAFHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ94</accession>
    <name>A0A1E3NJ94_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptide chain release factor N(5)-glutamine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.297</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_20947</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="1"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017328.1">
        <property type="nucleotide sequence ID" value="XM_019162176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ94"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ94"/>
    <dbReference type="GeneID" id="30178863"/>
    <dbReference type="OrthoDB" id="269872at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:peptide chain release factor N(5)-glutamine methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006451">
        <property type="term" value="P:translational readthrough"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050320">
        <property type="entry name" value="N5-glutamine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895">
        <property type="entry name" value="HEMK METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895:SF74">
        <property type="entry name" value="MTRF1L RELEASE FACTOR GLUTAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="6">
        <location>
            <begin position="76"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="264" mass="30223" checksum="C36CA26FBAEAFE2B" modified="2017-01-18" version="1" fragment="single">VKQARQEAVWITQELPQKEWLNASHLRSKLVPLQYILGSQPFGNLDISCKKGVLIPRLDTEEWVLEASDILRDVKIDHIVDYCTGSGCIGLGFASELRNTTQIECIDYKDEAVDLARKNHELNSSNIQTQIKFHKGSIFDKYLPSDANYLDERSHNMLVSNPPYIPEEDLISPEVEESVLRYEPRDALLGDSEFYEALCSEILIKNKSFKGFIFELGYMRQAQTVAKLLDRKLWNVGIRNDKSGKLRNVLGWKSNSPFTVLENM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJB2</accession>
    <name>A0A1E3NJB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017345.1">
        <property type="nucleotide sequence ID" value="XM_019159773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJB2"/>
    <dbReference type="GeneID" id="30176460"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="7"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="549" mass="61697" checksum="441439FBC7D09755" modified="2017-01-18" version="1">MSSSLEPKQLVLAKIKGFPDWPAIVVPADQIPAKLKTSKNAKFLDNYLHNDHVCVKFYYDDQYSWTNFASTKPLNDQIIDDYLISVGELNGGDDPASVPSTGKRGGRKKRITEAYLKVKQVPVDEFLEWGSWGKPVVPDLESELEAEEITPEYEAPGESKPVNRKKRAAASAKSEQSKKKQKATKKSTSTTTSKLVQKGGKKVGKSRRGAKDESETPDSVAFDEEEFIINDEAEGDDDYISSLDDSVSVEDDDEDADDADDFADEDENVDDAEDSGELLDDEEAGEVDNAQLEDEEGEEVWGLDTSKAKDLNVKFNEIPPASALAVEVAEMTAWCRELRAELQKLIFPLKKQKQGEKAEKKEEAQNDDMNGEKSTADSATLIPADTTSNQIAAELVKTEEAKEQNGMEEASEKAKEEDQEEDDRVIDYKKINKTIDGLVAPLLAADLSKSILKTTGLMKIINIILKKPELQTPTVRKLNKWWADNFHFKIDVDYRWGDDYTIEMHLQEEEERKEREEERKRRRKEERKSHSLAATPDVEDTSFSVKSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJG8</accession>
    <name>A0A1E3NJG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017329.1">
        <property type="nucleotide sequence ID" value="XM_019164153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJG8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJG8"/>
    <dbReference type="GeneID" id="30180840"/>
    <dbReference type="OrthoDB" id="10254665at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038170">
        <property type="entry name" value="tRNA_m1A_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="427"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="464" mass="52897" checksum="91A4DC8788ED64F8" modified="2017-01-18" version="1">MEPHRNITVDDYAILRLPSGARKMIQLRIEGVINMGKFGTFNVENVLGYPYGQAFEIGTENKVSAIESLEVGITDLAELDTDELVSGSSKDNRLLIDGANVQKLTVEEVEEMKNKMGGNEVAREIIQTMIKNHGSFDKKTAYSQEKYLDRKHKKFLRRFSINYLTSNEMLDYLYYEKEPFRIMNLSIEMLGSMMSFGNVMPGGNYLVVDETGGLVVYAMLERMGNRGTITLLHENDQPSVHLLNKTRYVKEISTSPESMVQCINVLQFLEPETERPDFKPLDEEEYKKLPEQMRTQYDRKLKRFHIINKVLDRVIENDFESLIYVSTLNPTTFVPKLLPRLRGSSPLVIYNQYKEALIELNHIFLREKGILLPNIHHSVVRKYQTIPGRIHPLMTSRSDGGYILTGIKVFPIENVVAIGRGTKKRRIAKANESNGDNSSGKVEETKGEECKASEALETAEEVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJJ8</accession>
    <name>A0A1E3NJJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="17">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_128046</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="17"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017435.1">
        <property type="nucleotide sequence ID" value="XM_019159969.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NJJ8"/>
    <dbReference type="GeneID" id="30176656"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000130">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1012"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1043"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1095"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1129"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1164"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1201"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="55"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="980"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1265"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1284"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="14">
        <location>
            <position position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="643"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="684"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="707"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="739"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="820"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="821"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="822"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="928"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="934"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="954"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="957"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="958"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="958"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ46322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1307" mass="148578" checksum="D689E94FBE2D2784" modified="2017-01-18" version="1">MKTVEEDVRGLLNKAYELSFKRWFDKSNRQPDADAVREIYYNQSLPEHMIDPKTHMNKILYTRNKIRTTKYTPLNFIPMNIFFQFTNIANSYFLFIIILGCFQIFGVQSPGMQAVPLIVIVVLTAFKDAFEDYRREISDYEMNSSKVHKLFGVDNHNAMEDHVGAWRRFKKACTRASRKVVRLFKRKRKSSPGDSNSTSNNSDLESADLYTIRSARSAMDHHSTRNSLSSSNYPLKFKSTQWKQVLVGDIVRIRKNEEIPSDILLLQSSTKNSICYVETKNLDGETSLRVKHAMSFSSSKIKRIADLGKLVFSVSTEQPNKNLYSFHGVVHYEDPEGVEKTSSEAASVENILLRGSVLRNTNYVIGIVLATGSETKIMLNSGVTPTKKSKISKNLNYFVLINFFLLFILCFVSGLINGLFYRKSSNSRQYFEYKPYAPTPQANGTLTFFVVLIMYQTLVPISLYITIEIIKTVHAFFIYSDVGMYYEKLDLPCMPKSWGISDDLGQIEYIFSDKTGTLTQNVMEFKKCAIADKSYGLAYTSAQEGYDKRLGNVDVTAKAKYMKELIANSRSQMIPQLKSSNPFFKEENLAFVSPEFMDEMNDKRNHDFLLALALCHSAVIDYEDMDSANDETIINYSAESPDEIALVSFARDMGVVFLGKEDDYYLVSDNGKQLKYKVLCVIPFSSARKRMTIVLESPDGNIKMYMKGADNVIMQRSARGEFDEEIGKNLEEYSNEGLRTLCISTKDLNPNEFYDWYDHYAKAGKLLDEQRELESERLADQFENSVVLLGATAIDDKLQDGVPETIESLRKAGIKIWVLTGDKVETAISIGYSCSMLNNNMDLLKVQSESVEDLERTIDALLKEKFDIDPTKAVNLKQARSDHSIPDERHALLIDGKTLTILFDESPKDVQLKFLMLGKKCCSILCCRVSPSQKASVVNLVKKNLDVITLAIGDGANDVSMIQAANVGVGIAGEEGRQAAMSSDYAFGQFRFLQRLLLVHGKWSFQRLSEMIPCFFYKNVIFVMPLFWYGIFTDFDGTYLYEYTFLMLFNLFFTSIPVITLATMDQDVPDYVSLVIPQLYRAGINYNERATILKFLFYMADGLYQSAISFFFPWLLFRTGSVATHNGLGIGQIFSVGTFCIHIAIISCNLYIILKQRRWDSITVSLNFLSDLLLFIWVSGWSSSHFSNNFYKIGLRNYESASFWACLFIGVLACVIPGFALDLFRINYFPSDTDKVREMLKINFFEQRDAGHVEKQLEIAKRIGNNSASGAGNDAETSKEVETPNMQQGTSESNEYDKSSDFSFQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJL1</accession>
    <name>A0A1E3NJL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_24760</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016871.1">
        <property type="nucleotide sequence ID" value="XM_019162366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJL1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJL1"/>
    <dbReference type="GeneID" id="30179053"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051229">
        <property type="term" value="P:meiotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904750">
        <property type="term" value="P:negative regulation of protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903024">
        <property type="term" value="P:positive regulation of ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="252" mass="28585" checksum="2592850958EB51E1" modified="2017-01-18" version="1" fragment="single">IGRGNFGDVYKAYDLRLQKNVAIKAINIEHSDDEIPVLLQEINLLRSLRNPNITNWYKTFIIDVTMFIVMEYCSEGSCSDLLKFNRNSLEESVLSYIMKNVLEGLKYLHSLNIIHRDIKAANILIAKGNVIKLADFGVSGQLNGNTGRKTFVGTPYWMAPEIVTDCKSLKKDQYDEKVDIWSLGITLIELGTGKVPNNDKEPLKALFEIPKMEPPRVPKNSSYYMKEFGVACLCKDPVMRPSAAELLKFKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJP6</accession>
    <name>A0A1E3NJP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EKC/KEOPS complex subunit BUD32</fullName>
            <ecNumber evidence="7">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25 26">Atypical Serine/threonine protein kinase BUD32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">EKC/KEOPS complex subunit bud32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PICMEDRAFT_73290</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="7"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016901.1">
        <property type="nucleotide sequence ID" value="XM_019164438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJP6"/>
    <dbReference type="STRING" id="763406.A0A1E3NJP6"/>
    <dbReference type="GeneID" id="30181125"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000745">
        <property type="entry name" value="Serine/threonine-protein kinase BUD32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22" id="KW-0010">Activator</keyword>
    <keyword evidence="19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="20" id="KW-0779">Telomere</keyword>
    <keyword evidence="23" id="KW-0804">Transcription</keyword>
    <keyword evidence="21" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="15"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00019973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00030980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00033194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="ODQ45788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="266" mass="30164" checksum="62897BC22BAD8E93" modified="2017-01-18" version="1">MSKEILDKVHTLLPGFEISLVSQGAEALVFVTAKHPYLPSNITPAKLETTKHYIIKYRPKKPYRHEQLDLQITKSRTASEAKLLHKLETLGVRGPKLLGLDAPNGIIWMEFLGFRLENNNFSSLKNWLWWIEGKGQAEAIGSQAKDVLREVGVAIAKLHMNDIVHGDLTSSNIVLTRKDNDALQLALIDFGLSSYSALVEDKAVDLYVIERALISTHPLYSQLYNEWLLEGYHSVYSDAKKTSQWVDVERRLALVRMRGRKRSMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJQ8</accession>
    <name>A0A1E3NJQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017497.1">
        <property type="nucleotide sequence ID" value="XM_019161145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJQ8"/>
    <dbReference type="GeneID" id="30177832"/>
    <dbReference type="OrthoDB" id="7832001at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017149">
        <property type="entry name" value="GSH_degradosome_Dug2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037237">
        <property type="entry name" value="Peptidase_WD_repeats_DUG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="983" mass="110071" checksum="78E1339D73D15928" modified="2017-01-18" version="1">MAWSSASMSKSHSTSSTPTSNLSPPTHNAELSSFNYHHEFSVSNSNLTKDHSSQFKKSLLHKWNYKHSILCMVASPRRKLLFCGTQESSILVLDLVTFQKKFEIPTHVGSVLCLHLSDCENILFSGGSDSLVKVWKIKDTQSRHNSRSSYSESQPTESTYDSFIQLIPTHTIYSLLDIGDIFSITWIDAIQSVFIGAQNASMSFVHLDTEINGSADEHLQFMPSNRFDKFFDSEKTKKSFSKSSSPLSFTETVPPETGSAQPSYTALHQHVKVIQIPTENITSYAHNGYLYALDFITIENPLTSTFANNIAENYQHVIVSAGGDGEIKLWGYSNENNGNLVFLRSLSHPKIDSIFSLAVSQSSLTVYAGSSNGNVCIWDLATFQLLKNFQIDPNDVYTLAVSPSTNGNYPQWLLMGTEFGITKMILSKSTVQVDMELENNDTGTCVRVTKNDPCLTLLTFAVNNESYLVSAGSDKTISLWSLHSMSNTCITCSDSLSLDNKLLTSGDSSVSGEKYENLSTYLTNEKFLQILGELITFRTVSKQPDTYKEECRKCANFLTLLFKSFGASKTQMFPVPNGNSIVHAIFKANSKKVENPGETPRILWYGHYDVIPASNSTWKTPPFTMTPNNGYIYGRGVTDNKGPLLVAIFAIAELHSRGELESDFIFVIEGEEEAGSWGFQNSVLENQYEISDGGKPIDWILLSNSYWLDDKTPALNYGLRGRIEIELVVWSDKPDRHSGVDGGVVIEPSMDLVKLLGKLVYRDQIMIPGFEEIYKRNKNEGVEGLKENLQYLEDWEIPLYQEIAQRVTDLDINELIRKWRLPSLSLHKIEMSGPDNGSVISQKAEAKLSIRIIPGQELENVKESFIEYVNECFKRLESTNHVEVKIMHEAEPWLGDVHNLAYQTLSTCLDEEWGVPPIFVREGGSIPSIRFLEKVFACSAAHIPTGQSSDNAHLNNERVRIVNLLKSKEIIKKAANRLPRAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJS2</accession>
    <name>A0A1E3NJS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribosomal RNA-processing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_72437</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017473.1">
        <property type="nucleotide sequence ID" value="XM_019164196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJS2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJS2"/>
    <dbReference type="GeneID" id="30180883"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043628">
        <property type="term" value="P:regulatory ncRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09862">
        <property type="entry name" value="PIN_Rrp44-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000010">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000001">
        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="16">
        <location>
            <begin position="84"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="RNB" evidence="17">
        <location>
            <begin position="510"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ46360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="978" mass="110901" checksum="89309D11C3DF3016" modified="2017-01-18" version="1">MSASVKRKRLASGLTVTQKVFVRSRNGNATKIVREHYIRDDIPCFSKACDKCIECYKPGPSGEVPHPILSESPLALTKGKIGKHYVVVDTNIVLNAIDILENDKVFYDVIVPQTVLNEVRNQSYPIYMRLRTLTKNEEKRFVVFHNEFKVETYVPRKQGEIINDYNDRLIRQTAKYYNNHLSDAGISVLLITGDKANKELAVKDGVMSYTIHEYIKLLPDSEELNDLLPSTENFEKQINEIKYPEYYSSARLMGGIKNGSLYQGNISISSYNFLEGSVMTSAIPKPLLILGRENLNRAFNGDNVVIELLPKSKWKKPSTQIIDEETIDKDGLGDDDDNEVIISDKERKLLTQEAIKAQTTENEDEKISPTGRVVGIVKRSWRLYVGQLSPNAVNKNEVVSNSAKNCFVILMDRSLPKIRIRTRRAKELMGKRIVVAIDSWNSNSKFPSGHFVRTLGEIEDKDAEQEALLLEHDVEYRPFSKNVLACLPKEGHDWNVADYISNGDPLLEKRKDLRDRLICSIDPPGCVDIDDALHARKLPNGNYEVGVHIADVTHFVKAGTPLDQEGASRGTSVYLVDKRIDMLPMLLGTDLCSLKPYVDRFAFSVLWEMDEDANILNVSFTKSIIKSREAFAYEQAQIRIDDKSQQDELTLGMRILLKLSKKLKQKRLDAGALNLASPEVKVHTDSETSDPNEVEIKKLLDTNSLVEEFMLCANISVARKIYDAFPQVAMLRRHGEPPATNFELLNDMLRIKKGLSISTESSKSLADSLDRCVDPKDPYFNTLIRIMATRCMMAAEYFASGNFSYADFRHYGLAVDIYTHFTSPIRRYCDVVAHRQLAAAIDYEPLHEIHMDKTKMDLVVKNINKRHRNAQFAGRASIEYYVGQVMKNNQSDQEGYIIKVFSNGVVVLVPQYGVESLIKLEVLGDVNSAEFDEDNYTLKFSDKSGKRRSVSVFDRVDVSIHSILDDYTGKRRVQLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJV4</accession>
    <name>A0A1E3NJV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynamitin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017535.1">
        <property type="nucleotide sequence ID" value="XM_019164213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJV4"/>
    <dbReference type="STRING" id="763406.A0A1E3NJV4"/>
    <dbReference type="GeneID" id="30180900"/>
    <dbReference type="OrthoDB" id="3994644at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15346">
        <property type="entry name" value="DYNACTIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="42685" checksum="0BD5EEBFF3414417" modified="2017-01-18" version="1">MTTADYYSEDVPVFENEPEVLESFPTADNNIYSQNKISERQFDPGLVIEEDTQVRLKSLSKDFETKVKEQLLYNFTNNTAAKSFRKKVNNQSSDARKNNRILKFTMLENELRSLMVEIDNDTESVDLNLKKKVDGLVNDIDHFKLGRPNTFLGYWEEKLNETRARENIDIKDWKEGNEKEQKENKIGNPKKITKDSDSIVLELESRISRLEDSVGYESSQSMTPTIQDTIEDLYTRVNLILDGGENLNLVETEVLKLIDNCETYIKDSKRVKDKSEIVPLTDRKLCLLYDKVKTLPDFASLLGKIVDRFSSLNDLIVGTANSVSFLQGLEKELSTMESRLDVWDEKIDLFSSQLAEDKRLFDERTKNIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKD3</accession>
    <name>A0A1E3NKD3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycosyltransferase family 15 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16455</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017717.1">
        <property type="nucleotide sequence ID" value="XM_019161210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKD3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKD3"/>
    <dbReference type="GeneID" id="30177897"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF7">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="55521" checksum="0D55A4176321C641" modified="2017-01-18" version="1">MPYVSRKFIRWMLVTVPALTIIYFIFVSERGIDPSSFDTSDLFGTNDGYGIEYNRKLMSEASTASKEIPVGGMTWEEVEHQLTFDQNLLKNIEDNMVEENMDFFREVYSKRITEPKFSKLTYNEYVNSDGVRVVLGKDEYPRANATLLTLVRNQELKDIIHTLRQLEANWNHKFHYPYTFTNDEPFTDEFKETIRRHTVSDCYFEVIPPEIWNKPSNIDPDIEREKISKLTEKGVLYIDKDSYHNMCRFNSGYFYHLERLKQFKYYWRFEPATDYYCSVDYDLFKFMEDNNKTYGFTISLYDNPLTVETLWPVTLDFLKENPQYVHPNGAFRWLTENVQHPDYTKTTGGYSTCHFWSNFEIGDMDFYRGEAYTKWMDALEEAGGFYYERWGDAPVHSVGLGLFEDRSKIHWFRDIGYHHSPYKNIPNSDKCNAPEDSGYFSPEDVYDQNCLSNWIRLEMTNKELQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKU1</accession>
    <name>A0A1E3NKU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16572</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017854.1">
        <property type="nucleotide sequence ID" value="XM_019161263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKU1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKU1"/>
    <dbReference type="GeneID" id="30177950"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12447">
        <property type="entry name" value="RRM1_gar2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000550">
        <property type="entry name" value="Nuclear localization sequence binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="171"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="276"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="235"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="350"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="415" mass="45303" checksum="A4874DC9616C5ADA" modified="2017-01-18" version="1">MAKADTKKSKKTASKKQEEKATKEVKAKKQEVSESGSDSESSSSSSSSSSSSESDSESESESGSDSSDSESEDEKKEEKKEEKKEEKKEEKKEESSDSDSDSDSDSEDEKKEEKKAESSDSDSDSDSDSDDEKKETSKKRKADESSEEESTTASPEPETKKAKTEDSSEPATLFVGRLSWNIDDDWLKREFEKYGGIISARVITDRQSGRSKGYGYVDFESKDVAEKALAEMQGAEVDGRPINVDLSTSKPRQQNTNDRSNDRAKKYGDAKSEPSDTLFIGNLSFDSDRDNVTEFFAEYGPVIGVRLPTHPETEQLKGFGYVQFSSVEDAQKALDNLNGEYLNNRAVRLDFSTPKPQNNNGGGRSFSGGRGGRDRSFGDRNGGGRGGRGGFGGRGGSGANSTPVTNFRGTKKTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLC9</accession>
    <name>A0A1E3NLC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_21433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018047.1">
        <property type="nucleotide sequence ID" value="XM_019162201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLC9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLC9"/>
    <dbReference type="GeneID" id="30178888"/>
    <dbReference type="OrthoDB" id="407630at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="169" mass="19502" checksum="271B6F4036C6F25F" modified="2017-01-18" version="1" fragment="single">MKEFSATQYFDYPWAYVSAANWRKYPNEVSTHVVAVDVLRREFDAEKQTLKSERLITCRQPIPKWLKAFVGGAETSYVREVSVVDRLGQTLTLRSVNLTMARILKVYETVTYSPDPRDPQHSTRFDQVARFKSSAGWKRVESQVESWAVERFSQNASKGKLGFDSILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLP7</accession>
    <name>A0A1E3NLP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73011</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018109.1">
        <property type="nucleotide sequence ID" value="XM_019164359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLP7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLP7"/>
    <dbReference type="GeneID" id="30181046"/>
    <dbReference type="OrthoDB" id="10263554at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.150">
        <property type="entry name" value="V-type ATPase, subunit H, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038497">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032184">
        <property type="entry name" value="ATPase_V1_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="6">
        <location>
            <begin position="363"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR032184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="487" mass="55577" checksum="1925917AD4ED84D5" modified="2017-01-18" version="1">MTALRPPALTPFRLEGDFVSQLLTNIRTRSISWTSFVKANYLSSENMAVLKSVTSVNDQEDPASRVDVLIKDLPTYSKVLLDSITDISKNKSKYLDLTKILLVILYDGLTFIDNSVPEHTMTHEIMKISKNNPYPPFVEILESSSSGEQDSNQDEFVISILCAHILIFFLTTNPPSSKKTAFELVSTVLKFIQDGLVTSSNAQYRFLGVQLLKELLSVKEFRDIYIKKTEYLDALIKIMLDKNVELQMRYLSIYCLWTLTFDSKTNQLLTTDAKYAEIIPTMFKFSNDAVKEKVVRLSISILINFLDVSSSSSKSQTEIIKKFLLANGLTIVKNLIERKWSDNELKDDLNTLYDLLNDSIKSLTNFDEYENEIKTKHLLWSPCHKNSEFWYDNIDKFKENNWKLLKSLISLLSDDNSAPDANVNQLYINQSIICYDLSMLIKVAPEVVKVINSFGTKTKIMTLMNSPNSNVKFEALRTTQLLVANSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLS0</accession>
    <name>A0A1E3NLS0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018187.1">
        <property type="nucleotide sequence ID" value="XM_019163856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLS0"/>
    <dbReference type="GeneID" id="30180543"/>
    <dbReference type="OrthoDB" id="3997597at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="210"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="383" mass="44746" checksum="63B8EC04D5B9B3C6" modified="2017-01-18" version="1">MLEKHAMEPPRKQNPCDIQFIPQLVSIQCLRMVLQRLKLWIRGSEDTSEGPTHFNTIDFDKGFSEDVYLPDDEVRNIHDRIRRCLDDEETGNNQEVDGTLRLMRDETERWYSESKNETFEPDLAPTNIVNEKYTDRECDGDINELVGYCLCLERNNAYLLQLIEKYEQVSFKKRYDELLTSNFKLQKEYTRLKSSNSKIYASYCDILEEIKKVKLDNGDLKKKVSQAREEFKAKKTETQKTEEEVKKLRTINDSNNHKLSELRKNSVTLQRHIMEKENEIAKLKGINIATKLKLERAENLVLKGQGNNDTEPKYTKEQKREVEPGVEEEQPVTDSGNGAAVKPDEKENYNSERTSQDAKEDDLEEQDTIALLLQKYQNKTFVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLY9</accession>
    <name>A0A1E3NLY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_23071</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017466.1">
        <property type="nucleotide sequence ID" value="XM_019162294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLY9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLY9"/>
    <dbReference type="GeneID" id="30178981"/>
    <dbReference type="OrthoDB" id="431626at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="5">
        <location>
            <begin position="16"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="908"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="949"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="908"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1012" mass="114704" checksum="72D802A667FDDB94" modified="2017-01-18" version="1" fragment="single">LLQNLMSSDNNVRIRSELTFKHMMESNPDAFLFSLIQIALNDSLELAVRQASLLHLKRAVPLYWSPAFDNFRGPNTINQEVKQTLRESLLKLIGDPDSKIRSSSGYAIVQIAAVDYPDEWPTLMDRLYTSTTNPQYSVYEIIGSLYVMQEIFDDVVTDEQFFEQGIAVQVLKTCEMLLEGVNYNLEIKVETLRLLQVVCNSLVDVDLEIPSRESFCHAVVPQIYQLLLNISRTVSESGQYVNQLMAWDFKHQLYTVLNFLVNAYAELLDRFISDSLDLALLDLSLESKVYTQLLTVEPMQSIFESIFVELVQALIEIHGLGSIEKISKLCDLLIPLSTLPFSKIDEYNADFNQFVTDESGLDGQITVRDSIRELLSDMNAKDNSIFIHILVQKFGHIQDFSMDQLGVEAVIFLLACCFDNDDTIVSQPPFDVNHFLNNVVELATNERLINEEFQFLVARLILMIPKFIFKYASICKALGIPSFEKISSIITELGGADDYLIIKSAILVSLQYYNYFIRAKEFSTDIQHKLLELVDQLKEDSEEDTNMMLLEVMTIIICIDNKSLSQNADSIHLILSIGFKNDSNFALNTSLFECIEDLISDIPRETYWNLVSSVFPSLLEKISEFDGEYNSQIDLSLQVISVFIKGQTGQEFSPQVFQSTFAVVTKFILACDEDALLQSGSECLIELVKNSVSLCSAYVDNETHETGIEILLKSVSKLLSPSMSDRAIAKLGDLVTVLLNQFSDSIDEYLEDILKALTVRLVKAKEVPTIENMILIFNMLTISQPQATINFLNSFTVGDEPALSKILPIWFQAYEIMRGYNSILSNVRAFMEIFKLNYPAVKHLIVDGDALQLQVPEGIIITRSMAKKVPVKYEKIPADAKIIKLLLDELKNEITSGKNIEISLKQGHGHDNYHESETGDDHDPHHNHFEDAEKEDDWEDMEGRGEATFDQLKSYVDDDGTTKRGSDATDNDMRQLLIEFFKECTSKNIADFESIYNSCLSETQKNFLSESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM06</accession>
    <name>A0A1E3NM06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15161</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018288.1">
        <property type="nucleotide sequence ID" value="XM_019160693.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NM06"/>
    <dbReference type="GeneID" id="30177380"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF196">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE SWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="997"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="136"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="289"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="366"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="435"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="716"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="764"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="817"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="895"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1055"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="451"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="764"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="778"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="817"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="838"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1063"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1024"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1449" mass="158280" checksum="47743650BDF99754" modified="2017-01-18" version="1">MSRHTSHLSEMTPSALNKTAFPQNTRKRLLHSPNSPNDDDNNNNNKTGSSSSSSKPGSTDSGHQHGHAHEDGKVSLRNKSKIKTMNKSVIANKIKNRMLLIASPMEPAGATGGAGASASAFADDYVDSDFEYDSDFDDGNSSGHGSHDHHDHHSYACSTPMHNRSATKSKHKSSSHSAQQDSRDPQDPRHRHTRSNPPSDADYTKLAKTTSQTSSIAGHQRVSLRRSTGLLNLSLETSPSTQRVINTSSKNDEMLKSFNELTFDNTTAAAAPASSISATTSAPGSASTVIATRSSSDSSNLSDLPLSTQSTRSKTNYKPTRVKYSSIFGTHNIANNNHTCTNRRGSDMQKKLERQLEHYKDKISSNAASSNFDGHIQSESSPPNIKVQSAKKTKSFTLNKFINNNTPFKSKSKISASLNLDHDLDLMVDDTSPSKHAYNKPASNNGSSNVLSTSMPMSNSSSLSNRPITTGSLANNYMNSTSMSVSSSIKTSNSNSMIPPSNLQSSISNTSNNINTFAAPSTFSSVKPLQTAFNSTGLQSKSGLTTFRRAKHTVPETPCKKPPRVVEIEKMRSSHQISGSTSAPATATTTTAAHTMTNMTISSTPLSNNTSFNSTANETSYLKNLSNITNNTTVSKHNLSGYKYPGPFTSNQYNPQPQAPAINTSDLQQCLLKFTNEFDDLYNEGNNNLSIFTDHQDLAGAEHSKIDLLKKMNDSISDDEIDNNGRNDDDDDDDDIKMWDESEKIPSTPREKSNMAINLSSVKSVKDSSAMNTPESFNSVGSVGSNSSGGKLNDTPTRRIGFYNKFNNFDNYNNTHANSNLPSSGSSYWQRANSRNPSVQSLNPRVSSSSSVNLIRESGNNNNNNNTSNTMGTKIPVFAPPNMPASLLVGLPKEGSNGRNHHSPHTPIESTFGKSPNMQQHALSFLNDARSGSICSTSSLISDNNGQTNMMTSTPMVGNDSKSTLKDMYCASSDTIVTTNSLTTHNPEIDNHLLSKFGNCALLGKGEFSIVYEVQFESVKYAVKRTKQRMPGPKTRLRKLEEVKILKALRRVGDDDEEDGNMGSYNDNSRQMSISGNSDGGNGKENEELFVNDGGDYVLTLISAWEIQSHLYIMTDYCENGSLDQFLINQCELSRSRLDEWRVWKILVEVMMGLKWIHSHGILHLDLKPANIFITFDGTLKIGDFGVGVKLPIPAFFDREGDREYIAPEIISRHEYTFAADIFSVGLIMVEVAANVILPDNGTPWQKLRSGDLTDAGKLSSSDLSECARFVNHSNSGSNNNNNSSSSASGVLNTNGDSIFSGNLESESTTRTDLSGSGSLYSSYMKPVNSSFKNGMLTGTGSSAQRSIVNTMDDSLSKKIEYWTPFWFYDGNSTLDKLVSWMINPTPEERPSSEQVLQSWECGLVELRRKSGATIYEGDYGPVVSRDEMEAERRILQTRGSFRLRDVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMR5</accession>
    <name>A0A1E3NMR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chromatin structure-remodeling complex subunit SFH1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71492</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018533.1">
        <property type="nucleotide sequence ID" value="XM_019163938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMR5"/>
    <dbReference type="STRING" id="763406.A0A1E3NMR5"/>
    <dbReference type="GeneID" id="30180625"/>
    <dbReference type="OrthoDB" id="10258327at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="49997" checksum="192641EB3183F0EE" modified="2017-01-18" version="1">MSSNITSKPLFPQALTTSFSHRLSQSNSALFSNLSTSRASKRQATSKVNYAEDYPDIDFDFEDSNNNNGVGGGSLNGAGGSGILNSGAGGVLVNNNNGGGTFSGSIIDGNHYGTINGLGDVLGGTGAAGGDVNVNANGASVSGNRDFEDDDEYVEDDDEKNDATYEDPNAAFDIYRNLILNSNNSSINLSNYHGNKEAPQDLPQIVFTPFEVLSNSANPEVIIPIKLKINTSGAVINDCFLWNLNETLITPETHAAIMSQELDLARNTESSIANQIKDQIQSYKDLLANPNNAIIDQFLSNEKEFHVVLDISANIGEDFYTDKIEWNLLDDTYTPEMFAKSVCNDIGLKPEFETAIAVAIYDEIYKFKRELIENPQQVSQNMDSLPFFNLINPDDASKSIVQGLRYDTKKYGEEFSPTVEKLSEWEIEKRETEKERNLRRRKRETLRVVSGVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMS3</accession>
    <name>A0A1E3NMS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15381</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018547.1">
        <property type="nucleotide sequence ID" value="XM_019160800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMS3"/>
    <dbReference type="GeneID" id="30177487"/>
    <dbReference type="OrthoDB" id="543373at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040928">
        <property type="entry name" value="Importin_rep_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18816">
        <property type="entry name" value="Importin_rep_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TOG" evidence="8">
        <location>
            <begin position="356"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1092" mass="121014" checksum="A7E257C92536D2E8" modified="2017-01-18" version="1">MSNIPPEIQEALGNLLSALSSTDNTYRKQAESALNNEWFTKDKIDVLLVFLAEQAANGADENTRAFAAVLFRRFAIKSPNYQGFSVTDRQVDHISHAAKAEVRRVLLQGFVSNQTKNVRHKLADAIAEVAKDDFEWPELLPTILQATTGDPVMKESSFRMITTTPQIISNHDLNESFLKMFHSGFEDSSDDVRIATCTAFVAFFENLPKSTWSILSQLLPNLLNSLPRLLSNGNDTALASVLESLIDLVILAPKIFKPMFETIISFCSEVSQNQELESNARLTALELLTSFSESSPNMCKREPSYSSAIVIVTLKLMTEVCIDDDDCAEWNNVDDPNDEGEEEEYNAARQSLDRVALKLNGQCLAGPLFQYLPHMLQSQNWRERQAALMALSSATEGCADVLISEIPKLLDLILPSLSDNQPRVQFACCNALGQMSTDFATVIQRSSGNRILPALISMLSTRNVPRVQTHAAAALVNFCEEATKDIVEPYLDDLLNNLLSLLKSSPKRYVQEQVITTIAVVADAAENRFIKYYDTLMPLLLTVMKSDVDHTNRSLIAKSIECATLIASAVGKEKFTESATQILEIFTYLQNNLQGDDDPVKPYLEKGWSRVCKLIGKSFLPFLPGVLPPLLEQAKATQDISIVEEEQLEEINQNEEYEVIQLSGKHIAVHTSVLDDKASAIEIIKLYAEVLDDAFVPYVSEIANEIVIPGLDFYLHDGVRGTCAVTMPALLKCCIDSTGSSQTAESYTLWKSMANKLIPQLESDPAVELFFAYYYALLKCLELMGPQALDDTQLISIAKSINCNLSDIYERINTRDDVDDDYNEEVSEYDDDCSDEELLEEVGKGISSVFENSGVRFIPGFMELTPTVNKFLCDENPILKRFAIQVICDLIKYGGNETFKLHELFLNPVGEALSSSDSKVREIASRCVGLCAQSPSSSDYSTFCLACIPILTQMTSIPDSRAPDNIDATEFMCSTLALIIQFYGDRISDVNDLISTWITLFPVCQNEEAARIAYTFLSQIINQHHPLVSTKVSQIVDSVVQALLYASISGDTAANVVASVKSLLATLPEDQSMAMLNSYSAEAKAVISKWFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN64</accession>
    <name>A0A1E3NN64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ketoreductase (KR) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_71604</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018659.1">
        <property type="nucleotide sequence ID" value="XM_019163963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN64"/>
    <dbReference type="STRING" id="763406.A0A1E3NN64"/>
    <dbReference type="GeneID" id="30180650"/>
    <dbReference type="OrthoDB" id="417891at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="26814" checksum="CEF9227500C3D4FB" modified="2017-01-18" version="1">MSIKPLLNQHAFITGGSKGIGSAIAAKLSSLGCRVTILARNQDLLKAQTDQLNHKYPLPNGEKHSYVKFDLLRPSELEKFISEGKCFHRTNILVNCAGMTQKTLLMTTSPQEIADIINVNLTSPIILSKLFVKSTSRVKDPGYNASIVNISSVVSVPGNNLTGASVYTATKAALTRFTQALAAEQHAIHDRRPLSPLVRVNVLLPRHIADTDIGKSVKTPSVGTSTAEDVAQAVANLVTDDHNVGGAATVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN99</accession>
    <name>A0A1E3NN99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018036.1">
        <property type="nucleotide sequence ID" value="XM_019161321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN99"/>
    <dbReference type="STRING" id="763406.A0A1E3NN99"/>
    <dbReference type="GeneID" id="30178008"/>
    <dbReference type="OrthoDB" id="1093at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054179">
        <property type="entry name" value="PSD13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035298">
        <property type="entry name" value="PSMD13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22037">
        <property type="entry name" value="PSD13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="192"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="400" mass="46277" checksum="C10D7D1A5E2F083D" modified="2017-01-18" version="1">MEIDSETVLVTLREEVESKDADLAAHFYTLEDYYERKLWYQLSELLKKEIYRNPNSRAIRLKLYDNFISSFSDKVNQLQLVEFLIISLADSSPENALEYLTNLKQTLLKRGEKKSSNFADQDMNDYEIIQALLYLENELAKVKLQLGFIDEATTIIEQSQEKLDNLSVSADNRVNASFYYIKAQLVKRKGDFNLFYYNSLLFLACIPDIDDLEDKENVVRDICISGLLGDKIYNFGEIIMHDIFKYLSDEWLKELLLSLNNGDLTSFNKLIANKTEIERIPEVSNRVEFLKQKMCIMAFVELVFNKPTTSRCIQYSEILQKIPLLVNGNEIEHLIMRCLSLGLIKGLINQVEENVEVSWIQPRTMTLAQIKGMKAKMELWSDKVSVLNKYMGQAGGEILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNH6</accession>
    <name>A0A1E3NNH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="8">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_71697</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="8"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018762.1">
        <property type="nucleotide sequence ID" value="XM_019163985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNH6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNH6"/>
    <dbReference type="GeneID" id="30180672"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990306">
        <property type="term" value="C:RSP5-BUL ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903577">
        <property type="term" value="P:cellular response to L-arginine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010994">
        <property type="term" value="P:free ubiquitin chain polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045723">
        <property type="term" value="P:positive regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010794">
        <property type="term" value="P:regulation of dolichol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032443">
        <property type="term" value="P:regulation of ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010793">
        <property type="term" value="P:regulation of mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000203">
        <property type="term" value="P:regulation of ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000238">
        <property type="term" value="P:regulation of tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000235">
        <property type="term" value="P:regulation of tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010795">
        <property type="term" value="P:regulation of ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08382">
        <property type="entry name" value="C2_Smurf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000011">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000079">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 8 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="13">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="13">
        <location>
            <begin position="289"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="13">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="14">
        <location>
            <begin position="433"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="130"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="229"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="156"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9 10">
        <location>
            <position position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="766" mass="87491" checksum="C9B49CD2781D67EE" modified="2017-01-18" version="1">MPAPRSKITVKVLAAEGLYKREIFKSPDPFAVLTVDGSQTQTTETVKRTSSPHWDQSFEFNVTENSVLIIQVFDQRKFKKKDQGFLGVINIRIGDYINFNDPSSEQTVAEELKKSNSNLPVSGKLIITISHKNGSRDSRDPTNFIRNSSVSDSTTRHSASNSASAHGTSSAASQFPGVAASNSSATSSSNARQFSSFEDQLGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDSNDNHKPSSATISSNSNNNNNSTVPVYGSSGQNITEEEASTLEATGQTVPGLGELPPGWEQRFTPEGRPYYVDHNTRTTTWVDPRRQQFVKSFGANSGYNAQRLSAMGPLPSGWEMRLANTSRVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKLVYFRSQPALRILPGQCHIKVRRDCLLEDSFREVMRQTPEDLKKRLMIKFEGEEGLDYGGVSREFFQQLSHEVFNPAYGLFKYASSDNYTLQINPNSNINPEHLNYFKFVGRVVGLAVFHRRFLDAFFVGAMYKMMLRKKIVLQDLESVDSEMYKSLCWMLENDITDILYESFSVEEDRFGEKVTIDLKPNGRDVEVTNENKREFVELKTEWIISKPVEGQFRSFMEGFNELIPQELVQVFDERELELLIGGLAEIDIDDWKKHTDYRGYQESDEVIQWFWKAVSEWESEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEIDQLPKSHTCFNRVDLPPYKDYDALKKKLTIAVENTIGFGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNI7</accession>
    <name>A0A1E3NNI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAGA complex subunit Spt7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15589</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018774.1">
        <property type="nucleotide sequence ID" value="XM_019160884.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NNI7"/>
    <dbReference type="GeneID" id="30177571"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05510">
        <property type="entry name" value="Bromo_SPT7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000032">
        <property type="entry name" value="Transcriptional activator spt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037782">
        <property type="entry name" value="Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="434"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="164"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="538"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="600"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1175"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1289"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="173"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="208"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="538"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="604"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1175"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1297"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1317"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1335"/>
            <end position="1367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1367" mass="155800" checksum="AAF81987A06E4E95" modified="2017-01-18" version="1">MSSMKKKRALEVFKAGDEKHLCNIAKTLFETGFFNCYLTPQQFNLLQKLLYFKGPNDEDNFAWSSFLNGSLLLRFKEDAGDDSNSNENMYMKPGYPTLRNLNATVRYLLYEKAVDYYFSQNLDETKEFQLLDNAVSDTHLRASFEDKNVENNIVGGVILTDMNNKPKVHNREDDDYDDDDDNDRNDKEDDNYDDDYDDDGVGDGDGDNNSKENRDEDRQSNDANETDRTSKEASKLEAHKSEPQEGLLLDDATKDYVLLVSKEELLKKPLYPSVPELSADEDAIGKMVHPILGTSSAIESSIEKQNELKLIKNSNKIYHNFENDLPNILKRRKLERSDKQLEMSEDIPQKDETGDTDSKQVNKLMTLGGAVNLSLKNLLSRIEENRDKLGATDVELKNLIMDVRKNRSKWANYNKIGQEELYEACEKVVTELRGYTEHSTPFLNRVSKREAPNYYQVIKKPMDLNTVLKKLKVLQYQNKQQFVDDLMLIWQNCLTYNSDPKHFIRIDALAMRKKTLMLIPLIPDITIRDRTVVEREAAEREKEKEKEEGQNVGRTSARGVGHKKAKKGRKGADVESPAASIIDASDSAATTLDDTPAEIKVTGTPMSFTPLNTEQNNTNTPSNLADDDDAMDIDEEDEEDAVNNIDENMAELNAEDVNEDADDLEMSTWKTLTSTTRYKLCNERSKLFKDNKIQPNTEATIRTKSQMYNFCRYLEDESKIVLHRSRKYFDENDDPYLIEYDVAGGVPSIKHQNTNFDSIEESILEKMISDGKTLDDLGPSGYKVKLDGSNSLVLDNISLMQDIRKVCFKINLIRTMQTSQFVHQSQFVAPNYPKIKFEDIDPMSKLATRDLMTEEIASHSLQKSVSAIAMANGFQSTAPSCAVLLTEIAKVYMCNLAKSMKLHLESNSINKMPIKGNKPVTCRDVLQLVLEYNGVEKPDVLYSYYKEHMTKQNRKLIDLKYSLENFLRDLLRPGMQDISETQFSDDSEQFVNGDFSDEIGEDFFGFKELGLDKEFGLLTSNIPLHLLQSKLSYQFNQMNKHMAKRVYEDFEEVEFPKLCKRDIPKQIGILQPFYEDVYIKSKLMYNKQLKKFQTQTLNGETPEEPFHSIDNEEELVLIEDNDLPHKQRNNRPKIPPNGKITQIKKKFIATAFFVEKQDEIECRLESMIKKKESDAQKEMKKKKESEDEQQAADVDGSFELKTNDISSAVNGDGKKEEGKLNFSNSTENLSALDNTTDINKDGLVRTVGAPIEISKTENETAAKELPVNNDDSDEISAGIKSLSADVILGNDNTGVEGKGEEKKLRIDNGMFSIEGQSDDKGTAEVDTESRLDKEEEKEEEMDEEMDGDMGDDMDEEIDDEDGSDDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNJ3</accession>
    <name>A0A1E3NNJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_58077</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018777.1">
        <property type="nucleotide sequence ID" value="XM_019163275.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NNJ3"/>
    <dbReference type="GeneID" id="30179962"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056541">
        <property type="entry name" value="Ig-like_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056542">
        <property type="entry name" value="Ig-like_POM152_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056543">
        <property type="entry name" value="Ig-like_POM152_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056544">
        <property type="entry name" value="Ig_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037701">
        <property type="entry name" value="Pom152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056540">
        <property type="entry name" value="TMD_POM152"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206:SF1">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24312">
        <property type="entry name" value="Ig-like_POM152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24519">
        <property type="entry name" value="Ig-like_Pom152_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24527">
        <property type="entry name" value="Ig-like_Pom152_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23664">
        <property type="entry name" value="Ig_Pom152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24097">
        <property type="entry name" value="TMD_POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 N-terminal transmembrane" evidence="3">
        <location>
            <begin position="28"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 first Ig-like" evidence="5">
        <location>
            <begin position="184"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="2">
        <location>
            <begin position="552"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="4">
        <location>
            <begin position="766"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="2">
        <location>
            <begin position="885"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 ninth Ig-like" evidence="6">
        <location>
            <begin position="1112"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="4">
        <location>
            <begin position="1207"/>
            <end position="1275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1282" mass="146601" checksum="B42DFF8984FBE689" modified="2017-01-18" version="1">MEAAKNCTDRRKSHVINVNPVISESILDVDSQRYYVWAIFILIQAWKLYDLYLLHNTNLFDAAHTDELEWLHGFGFLHPKLGFIFKYFFFDSLLILIIPFLNIPKLCFTPMLSVFLLCLVNLSTVVLTCSFSFTFSSIMYSIYRTIIPEKELAIMETYVDTDSIINQSDHFKGRKTIRYAPDSSIKINPFGQQFCIRPVYNDIIKIPIKLESTYDLNFLQINYHDFNNDDSVLNYTQRELKTFIVGDYYNSAYVKYDPSVLADNNVQILEIPVQKPGYYSIKVATDKKNKIIRSYRSDTVVPVCPEAAFMPKESFSLDKCLDDVVDDLQITLLGVPPFTLYYEEEINGELSRLPPTHVSHVEKVDSPLNSKDLMQHKKVRYASNYLQDISWAKSYNISVPIGEKKLQKSGKYIYTINKVIDGFGNVISYTPDPNDKLSFHSFYCHPKPIISLVDSKPAIPILVDSQKYLDVRISNYGSMNRESPFEVVFKYIPNENENLYDPTTFFQTFDLNSNSDLKIMVDKPGTYMIEQGSSKFCPCKLGPSSLNILSAKLPKMSVSLDPIIDNCIGTIGFKFNIDFVGNAPFEIGYQISKLDPNDSNRVLRVEKLSSIQSDSTTLEYVFKPSSEGSYSIEFTTLSDKFYKNRIHFEQSEYRYITYFKQKPRAYFSKGSKVQRLQCCHGAPSQTTLNIEGKAPFNVTYDIISPDYSVQSYTLEDIFENQILIKTPEFVKGGEYILTLRSVSDSTDCDVEFKGQEVHIDVKNDVPQLSFQRDETFDIVKGDFFTVPLRIQSNDLIDLVYSYQSFDGQVIDTITLNHFNPVKGLQLSRDGVYRLVSFTQGKCVGRVIHDYAIKINYLSLPSLKIDQHNTELIVEKNNVFKKLKVCQNQNAQIIFNAVGSTPFAIKYSVKYPSGKVEERIEQINGATFTLQLSTNIPGSFEYKIRNIYDSIYTEEILNRLERLGSYKFDDIVLKHEVTSLPVAKFIDTNTKIQTCISLLDETAKLPPIKVELTGALPMSMKVDIYNEYDGSLETAEFADLDSSVVDLMVLYRYLGIGTHVITINQVFDANGCSSDENNSKEEAITIQVNDVPKIRHLIEESNQLSDFDSTADGHYYCVGDQITFMLNGVAPFQIYYGFNNALQQVEVQGNYFKRRAPGPGELQIKSLRDSSAKDCKVDYNDFDRKDLKAIIYDLPSVEIVQGDSIEEDIHEGEQVEITFLLTGTPPFKLTYIRKELEDSSKIVETEIVEDIMTNEYHILANLEGTYEAIEIQDRFCVARNHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNP3</accession>
    <name>A0A1E3NNP3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018827.1">
        <property type="nucleotide sequence ID" value="XM_019160896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNP3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNP3"/>
    <dbReference type="GeneID" id="30177583"/>
    <dbReference type="OrthoDB" id="4026704at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="466" mass="51022" checksum="D4E8F0C0AE0474FD" modified="2017-01-18" version="1">MTGGPALNLDLQQTTMTAQPETMHCKTQHHSHSQKHGDNHKANYGAKPDLNQQQQHEAVDVACPPAPEQEQMRPGLPDFYRQGSTPHRSQHGHEVHISPRTSHVGSFAADSQPGLDFKDSPTRAAREKERAADYYSYGSGTGNRLAHRRSWSRASSRANSRRSSVSGSGNISSNMATLSISPIGTPIDTNALDAMEKEQEGIVMRLMHEIQTLKDENKMLKNMLRTSSVSSANTESSPSPISSGSSVSSLSSPSTPFMSQQRRATFTTMTNMKRVPANLSYANSNGSVTNVSTFPRSRSGSLSMNWKASTLSPKLSGVPNITLTESFPFDTEYFSLTPHRSSFRNPSGALSLSIPHKILSYSNNNEEAIDDANLDQPWESPHDNHQPPQQRQPITKGSVEDSGPEPSKLKPFKKLHKRSVSDTTDCSISKQYKLDSLAIQQSRGREGKPVDGTVSSGLMIRHFGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP45</accession>
    <name>A0A1E3NP45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15274</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018416.1">
        <property type="nucleotide sequence ID" value="XM_019160745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP45"/>
    <dbReference type="STRING" id="763406.A0A1E3NP45"/>
    <dbReference type="GeneID" id="30177432"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140455">
        <property type="term" value="P:cytoplasm protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140454">
        <property type="term" value="P:protein aggregate center assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035719">
        <property type="term" value="P:tRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000048">
        <property type="entry name" value="DnaJ family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000036">
        <property type="entry name" value="Type I HSP40 co-chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="125"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="125"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="369"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="43987" checksum="016AAE178753F99F" modified="2017-01-18" version="1">MVADTKLYDALGVKPDATEQQLKKAYRLGALKYHPDKNPSPEAAEKFKEMTAAYEILSDSEKRDVYDQYGEQGLNGGGMGGMGGMDASDIFSQFFGGGGGRPSGPVRGRDIKHSLGCTLEELYKGKVAKLALNKTVLCKDCDGKGGKNVKKCTGCNGTGTKFVTRQMGPMIQRFQTQCDKCNGQGDIMAESDRCKTCKGKKVTNERKILEVNVYPGMKEGQKVVFEGEGDQQPDVIPGDVIFIIEEKPNELYTRKGDDLYINQKIDLLTALAGGEVAFKHVSGEWLKLEIIPGEVIAPGSTKVVSGKGMPIPKHGGFGNLLVKFDVEFPKNHFASEEQLKALEKILPARPVQKIPKGVEVDDSCTFEEYDPLKHAPRTSNRGGYYDEDEDEDGGQPGVQCAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP79</accession>
    <name>A0A1E3NP79_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018456.1">
        <property type="nucleotide sequence ID" value="XM_019163919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP79"/>
    <dbReference type="STRING" id="763406.A0A1E3NP79"/>
    <dbReference type="GeneID" id="30180606"/>
    <dbReference type="OrthoDB" id="2405700at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038910">
        <property type="entry name" value="Hua1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28031">
        <property type="entry name" value="PROLINE-RICH PROTEIN HUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28031:SF1">
        <property type="entry name" value="PROLINE-RICH PROTEIN HUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="279" mass="30330" checksum="D14F73380BC29C6B" modified="2017-01-18" version="1">MSEKNSSGKYSHTPSANELNVFDPNVDLPSEPPPSYDEAMADPSVSEDDMGPQTPLRPPQTPSRRLSQSGEQYSKYSRPVAPPPNMSGNSNRLTVPGMTSTSSSTASSLSSSNHSYTHQQPQRPSAPPPQHKTSQLPKPSPINPNPYLPWRYPSSYRCSKCENTGYKKKNGHACKTCWGRFRPQTTPPATKAELERLVKHRPAPNPINTNVVKLPPGATVMPTGPRVQAPTVVKPGDPRIGGILCPKCNGRGMVHFFLDLERCSKCNGLGRVGSNGRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP97</accession>
    <name>A0A1E3NP97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_114530</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018641.1">
        <property type="nucleotide sequence ID" value="XM_019159797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP97"/>
    <dbReference type="STRING" id="763406.A0A1E3NP97"/>
    <dbReference type="GeneID" id="30176484"/>
    <dbReference type="OrthoDB" id="272624at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001156">
        <property type="term" value="F:TFIIIC-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039467">
        <property type="entry name" value="TFIIIB_B''_Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929:SF0">
        <property type="entry name" value="TRANSCRIPTION FACTOR TFIIIB COMPONENT B'' HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15963">
        <property type="entry name" value="Myb_DNA-bind_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="3">
        <location>
            <begin position="607"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="704"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="472"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="757" mass="84185" checksum="266599F9430FE790" modified="2017-01-18" version="1">MSSINKSGKRFTPKIKQRTRRVPGLMTPQSSQTVQLARTPQASPISVAVPTSPISQKIADEFASSTKEEELKEEPVLNQASTSIQNDNIIHGDEEVKAGGKRETKKGDVFAETEEESQAVELKGKRELSSVSSLGLESETERLISGQQPKVKESNGIKLQERKSSVTGSGHLFTQPSSIVHRTRLNSLSIPTPLSTAQSTSRRGSVVSASNSRRGSVILPSANPIAPSGLGSRRGSVIGGLGPITPGSRRGSVNIRPVFVPIGGGDRSRRGSVTINGDEKPTPIPILKTNKKRRASVNYGPKKRKDVNVGGISIGGSLAIKKSDFSSLNTSRSGANKENGNGLMDSDIEIGDPRDMTTIRKVGAANNGDGNEVSGKESEPVKEKKLELTEFEKENKQKWLLDRKMNKFVLVDTEPLVKDKSKQSDFELEHEIESLSELEQMRKANNAKLGGSLKFNAKKIKLEDLCKPFLPVGELSNQYERAMEAEKRLQEARVKRREIREKARQLRMSEKDVMKLSESGASLSTELSEKERLQKVKELMEKDRTEEHKKHVVPLLQIQGNKLTYSHESTVVDRHEESNTEMEKVEENPFENIVTSSSYSRRTTTLRWTPQEVAELLKAISLWGTDFGLIAKLFPHRTRKQIKAKFLLEEKVHPHLIEFALLRKLPVDVREYSGKTGIEFKTLDEYNQQIQELKLKHEEELKAMAQAREQAHAEDRAQQNLHGDGSQGVPTRRSRKAVIAEFRKNEEIVGSIESGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPD6</accession>
    <name>A0A1E3NPD6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
            <ecNumber evidence="5">3.5.1.2</ecNumber>
            <ecNumber evidence="20">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">PICMEDRAFT_15498</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31 32">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="25">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EC" id="3.5.1.2" evidence="5"/>
    <dbReference type="EC" id="6.3.4.16" evidence="20"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018681.1">
        <property type="nucleotide sequence ID" value="XM_019160850.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NPD6"/>
    <dbReference type="GeneID" id="30177537"/>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000011">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000036">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000009">
        <property type="entry name" value="Carbamoyl-phosphate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="29">
        <location>
            <begin position="556"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="29">
        <location>
            <begin position="1094"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="30">
        <location>
            <begin position="1351"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1878"/>
            <end position="1908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="ODQ47568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2224" mass="245705" checksum="9EA034DA3C62C711" modified="2017-01-18" version="1">MTFHNPISPSFEKHGTKLVTLETQDGIAIQGYCFGAEVPAAGELVFQTGMVGYPESITDPSYEGQILVITYPLVGNYGVPDRKLMDDVVKSLPKYFESNKIHIAGLVVAHYTEDYSHYLATSSLGQWLKEQNIPAIYGVDTRSLTKRLREKGSTLGRFCFLKDSVNVEVGLKDSEWKKNFEIPQWIDPNVENLVAKVSTKQPVIYDPPKELAKLGPDGKVLRIVAVDVGMKYNQIRCFVNRGVSLKVVPWDYDFTKEEYDGLFISNGPGDPSVMTDVVERLRKSLDEAKTPIFGICLGHQLMARASGASTLKLKFGNRGHNIPCTSTVSGRCYITSQNHGYAVDVKTLQPGWKELFVNANDGSNEGIYNTEKPFFSVQFHPESTPGPRDTEFLFDTFIQSVIDFKTSKALKAVEFPGGKKEENEKLYPRLNPKKVLILGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILVNPNIATIQTSKGLADKVYFLPVTPEFVRKIIKHERPDAIYCTFGGQTALNVGIKLKDEFESLGVKVLGTPIDTVITTEDRELFARAMDEINEKCAKSETASNIEEALAAVQRIGFPVIVRAAYALGGLGSGFADNEEELVELCNKAFAASPQVLVERSMKGWKEVEYEVVRDAYDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPHSKEYCIIEVNARLSRSSALASKATGYPLAYTAAKLGLNIPLNEIKNSVTKVTCACFEPSLDYCVVKIPRWDLKKFTRVSNLLSSSMKSVGEVMSIGRTFEEAIQKAIRSTDYQNLGFNSTEALMSIDIDSELQTPSDQRLFAIANAMANGYTVEKIHQMTNIDRWFLGKLYGLTKFGEYISSFGTKENLPIRVLKEAKQLGFEDRQVAKFLNSNEVAIRRLRKDAGIIPVVKQIDTVAAEFPAFTNYLYITYNGQDSDVDFNDHGVMVLGSGVYRIGSSVEFDWCAVTAIRTLRQQGLKTVMVNYNPETVSTDYDEADRLYFETINLERVLDIYEIENSAGVIVSMGGQTSNNIALQLQRQNLKVLGTSPAMIDSAENRYKFSRMLDNIGVDQPAWKELTSIDEAEAFAEKVSYPVLVRPSYVLSGAAMNTVYSRDDLASYLSQAVEVSPDYPVVITKYIENAKEIEMDAVAKDGKLIMHVVAEHVENAGVHSGDATLIVPPQDLAPETVKRIVEATAKIGKALDVTGPFNIQFIAKNNEIKVIECNVRASRSFPFNSKVVGTNMIEMATKAIMDLPVTPYPTQKLPDDYCAVKVPQFSFSRLSGADPVLGVEMASTGEVACFGHNKYEAYLKSLVSTGFQLPKKNILLSIGSFKEKQELLPSIRKMSQLGYSLFATAGTADFIQEHGIPVKYLELLNEEEEDQKAEYSLTQHLANNLIDLYINLPSANRFRRPSSYVSKGYRTRRMAVDYAVPLVTNVKCAKLLIEAIARNEPLEVDNIDAQTSHTTIQLPGLVSIGTFVPTLGEAGAAVFEETTKASLAAGFVFNSIMPITAEGSSVVDARTLTRATEVIYGNAYSDYSLFVAATETNAPHLQQVSALTSALYINAEDFDPSVIPSLASHFGVWPTNKPIIVNAKATSLASLLLLASLFNSKIHVTNVTGRDDLALISMSKQKNLNVTCDVAIFSLFFSQADYPGATFLPTKEDQKALWDNLESIDCLSVGATPYKLAEFLKKPVSAGFGFADSIPLLMSAVTEGKLTVANIVTKLHENPIKILGLSESESYIDINVDRKASTLKSHIESWSPVKNDTGSVERVYMNDQTVCLEGAFVVQEALGKESIAQHTSSIANESAIEMSSPAPLGVMSPNLRRGSRFSFDVTRRHSLIDSLKDGEDEDAPSGADLVSSKPPRELSPPNAISTYIKGHNPFLRKNILSVSQINRRDLHALFTVAQEMRLAVEREGVLDILKGRVLTNAFFEPSTRTASSFNAAMQRLGGRVVSISEQGSSVKKGETLQDTIRTMACYSDAIVLRHPSEDSADIADKYSPVPVINGGNGSREHPTQALLDLFTIREELGTVNGITVTFMGDLKYGRPVHSLCKLLKQYQVKIYLVSPEELRLPSNLRDMLKEEGMLVGESTELTTEIIAKSDVLYCTRIQQERFKDLAQYERLKGTYVISNKVLKDAKQHMCVMHPLPRVDEIKEEVDFDQRAAYFRQMRYGLFVRMAILAMVMGVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPE1</accession>
    <name>A0A1E3NPE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019093.1">
        <property type="nucleotide sequence ID" value="XM_019160991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPE1"/>
    <dbReference type="GeneID" id="30177678"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="57" mass="6837" checksum="5EF9E72A62D09D71" modified="2017-01-18" version="1">MEMMKKEEEDEDEDEDDEEKEKKKKKKKKRVVTEKILFSKTMVTPCRDLKFVPTSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPE3</accession>
    <name>A0A1E3NPE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit delta, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">F-ATPase delta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_15363</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018526.1">
        <property type="nucleotide sequence ID" value="XM_019160792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPE3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPE3"/>
    <dbReference type="GeneID" id="30177479"/>
    <dbReference type="OrthoDB" id="270171at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.15.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit delta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="entry name" value="F0F1 ATP synthase delta/epsilon subunit, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048938">
        <property type="entry name" value="ATPD_C_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="ATP SYNTHASE DELTA/EPSILON CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822:SF7">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT DELTA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21334">
        <property type="entry name" value="ATPD_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP synthase F1 complex delta/epsilon subunit N-terminal" evidence="14">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="F1F0-ATP synthase subunit delta C-terminal" evidence="15">
        <location>
            <begin position="114"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="156" mass="16561" checksum="79EAE6C8DF26A8A4" modified="2017-01-18" version="1">MFRQSFRAITKVNRLAGVRTYAEAATTLKLSLAMPHDTIFNKKEVTQVNVPAVSGDMGILANHVPIVEQLNPGVVEILEGTASSKFFISGGFVSVLPDSSIAITTVEAFPLDAFDASTVKTLLTEAKKNAASSDEKVAAEASIEVEVLEALTTALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPJ0</accession>
    <name>A0A1E3NPJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72007</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019122.1">
        <property type="nucleotide sequence ID" value="XM_019164072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPJ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPJ0"/>
    <dbReference type="GeneID" id="30180759"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034276">
        <property type="term" value="P:kynurenic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000024">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="36"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="424" mass="47595" checksum="54623EC5A5EF3127" modified="2017-01-18" version="1">MSALPKNNPYFSLGGKDIWSLINETAAAKEADSGEKVANLGQGFFSYSPPEFAIKAAQEAFTNPMDNQYAPSAGKPQLIEALKSHYSKKLGRELSSGEVVVTTGANEGMLAAFFGFISPGDEVIVFQPFFDQYISNIEMPGGKVVYVQLHPPADFDQRVVDGSDYTIDWNELERAISPRTKMIVLNTPHNPIGKIFTREELDKIGKLAVKHNFVILSDEVYENLYYNKEFPRIAASEDIEVQRRTLFVGSAGKTFAATGWRIGWIIGSKELIPYVKAAHTRICFSTPAPIQVAVSKAIAIADSNGYYEQMREQYRKKYSILQSVFDELELPYTKADGGYFLLVNLKSVKLPELEWPELIANKPRDFKLAYWLIQEYGVVSIPPSEFYLPENAAVMSDCLRFAVCKDDEVLEDAVRRLKGLKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ8</accession>
    <name>A0A1E3NPQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71737</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018811.1">
        <property type="nucleotide sequence ID" value="XM_019163993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NPQ8"/>
    <dbReference type="GeneID" id="30180680"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="61877" checksum="DD1E009B52E792F0" modified="2017-01-18" version="1">MSSTKSFKKTIAKFFLPDNPKDFKTEYNHPEVDRQRREPKTSKYDKFNFLNAFNWYPSHYSKFERTFLVKFDICVLFFLCASFYTKYLDNSNVGSAYVSGLKADLNLHGNELNYFNTCYTVGYALFQIPITLLITKQMYVIRFFVGVFEACSFPATYVILSSYLTEEELFKRAGVYGAFATAGSASAGALQTQARRSLDGVFGLAGWRWQFIIDSVLTFGIFLYGFFLFPGIPSACKEFGVFSEDEMIFARKRLENKIAFPDKFTKKTLIETLKTWQIYFASFLWVLHHLSWYSNGPKLYMKARTDIYTTSMVTTWDSYIYCVGIPCSIIVPPLCQYYGKLIPVNLVMLTAYYACIILVTWNVPNAVMISAFFMQEPFRSGLAQNFYSWIASLCKDNVEKKAIVLSWVQALSYGINAFAVTLQYDLSGAPRFKKGYIVNFVIIAATHVVFFIGLFLDKYDRKYIPQFAGNRHTDGDEKLLSTVDVAGDSDSDSDVDSDASRVAPGRLGHLEYLNTEKKQSDGDDAIHVVTYDGSLHSGSASSSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPU3</accession>
    <name>A0A1E3NPU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RecQ mediated genome instability protein 1 OB-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019223.1">
        <property type="nucleotide sequence ID" value="XM_019164098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPU3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPU3"/>
    <dbReference type="GeneID" id="30180785"/>
    <dbReference type="OrthoDB" id="341511at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="1">
        <location>
            <begin position="45"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="217" mass="24834" checksum="B9ED01CAA748444A" modified="2017-01-18" version="1">MPNFLQEDIYKGKSTLTFHEPRRDCKNYLVPFRHCLAEPTPDDKLHDTTLFQIVDLRDITKSVLSMIEGLKLLIDINESFQDRQRAITGPGASKKQNSKPRATIDAVNADEELGHDGATPYGGWSSSYRLTLQDCFGNTCYAYEVEPLGFLRKRSNGFFPIKLGSKIVVNSGTKIIFNTLQLKNENVEFLGGYIMKLDYKLYERKLKELKEEIHYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ50</accession>
    <name>A0A1E3NQ50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P/MRP protein subunit POP5</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72183</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019324.1">
        <property type="nucleotide sequence ID" value="XM_019164117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ50"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ50"/>
    <dbReference type="GeneID" id="30180804"/>
    <dbReference type="OrthoDB" id="24745at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR023803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="168" mass="18635" checksum="8015C95D34A9B295" modified="2017-01-18" version="1">MVRLKTRYILFQLRYPQEVDETGVGGRGLPCDVPTESTSTLSARTLASTLRLSMTKNFGDEALADTLTSFVVKYFSNKTSTGILRVHRDAVERVLAAMFFVQTVEGRDVIWESVGVSGSISKCEKRAITRNRRVIRELRRAGGTEAEVDMAEAMLENLRGAGAEDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQB1</accession>
    <name>A0A1E3NQB1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019368.1">
        <property type="nucleotide sequence ID" value="XM_019164129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQB1"/>
    <dbReference type="GeneID" id="30180816"/>
    <dbReference type="OrthoDB" id="2430343at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="110" mass="12408" checksum="3DE18B8DBCD7FEFA" modified="2017-01-18" version="1">MSLPVTIPDLRFEETFKAKLIKNAIEENQAKHTTKDGEIDKHISTGLILKTLFVDQIVMPFIQSFLLSSLLFYIRPLVTVSARKGHTLGVSLVSFIRSTGRTIFFLNPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQE3</accession>
    <name>A0A1E3NQE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal protein L11 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_80145</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019442.1">
        <property type="nucleotide sequence ID" value="XM_019164981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQE3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQE3"/>
    <dbReference type="GeneID" id="30181668"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="6">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="17613" checksum="D63BDBC7DAABE60F" modified="2017-01-18" version="1">MPPKFDPNEVKYLYLRAVGGEVGASSALAPKIGPLGLSPKKVGEDIAKATKDFKGIKVTVQLKIQNRQATCSVVPSASSLVITALKEAPRDRKKEKNVKHSGNVPLDEIFEIARTMREKSFGKNLASVTKEILGTAQSVGCRVDGKPPHAIIEAIDAGAIDVPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQM0</accession>
    <name>A0A1E3NQM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SUN-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_30043</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with EMP65.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SLP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019520.1">
        <property type="nucleotide sequence ID" value="XM_019162623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQM0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQM0"/>
    <dbReference type="GeneID" id="30179310"/>
    <dbReference type="OrthoDB" id="266334at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000099">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="13">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="161"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="370" mass="42540" checksum="E49D043BB4104B4E" modified="2017-01-18" version="1" fragment="single">MFAGSGEEQGKLYKDRFNYASFDCAATIVKTNKEAKGANSILLDNKDSYLLNECSASSNFIIVELCEDILVDEVLIGNFEFYSSMFKNIKISVSDRFPTTHWIVLGEFQAENVRQLQTFGIENPLIWAKFIKIEFLTHYGNEFYCPISSVQVHGTTMIEQFKEDNPDNEEKPDSELISTTEEVNAKPNSLDSYQESLPHDLPNDTLANKMEGVTSEEQSKVVETQPHTPSPKVQPQDSIYKNIVKRLSLLESNTTLSLLYIEEQSRLLSDAFTSLESQQAVKFQNILSQLNSTIQSQMDIFQKLNVDVYTSFSRLFEYQQQNFESNNVEISKQLSQIHNMISFYRNLTYFCLLTIILLFFYILLTKDLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQR7</accession>
    <name>A0A1E3NQR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha</fullName>
            <ecNumber evidence="4">2.5.1.58</ecNumber>
            <ecNumber evidence="3">2.5.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CAAX farnesyltransferase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FTase-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ras proteins prenyltransferase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Type I protein geranyl-geranyltransferase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_31702</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="4"/>
    <dbReference type="EC" id="2.5.1.59" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019026.1">
        <property type="nucleotide sequence ID" value="XM_019162713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQR7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQR7"/>
    <dbReference type="GeneID" id="30179400"/>
    <dbReference type="OrthoDB" id="272289at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF1">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE_GERANYLGERANYLTRANSFERASE TYPE-1 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="339" mass="40002" checksum="6D1C4186DBAE2497" modified="2017-01-18" version="1">MNNDKYNFDDITPLPVSDEAPETKDVVLCKIMYTVEFQTVFSYLRALMAKDELSERAMYVACCAISIVPAHYTVWEYKYRIVEKLVYECKYDVDEELKWCADLALNNEKNYQIWNYRERVIELKIKHGLGGDKLQYDLDGEYEVVRQMLDSDEKNYHVWSHKRWIVSYFDLFRSDRELAYTQELIDRDVRNNSAWNFRHLVVFGDESPLENGRLTSEVEFAKQYIASSITNPSSWNYLKFLYSFCTKARALGEPEACACVALIRTLASEYSCNENLDDDFSKVLEEQRLSVPAFELLGDIYRDEGKMEQMRKVYRLLGEELDPVRRNYWAYRTSLCQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQT7</accession>
    <name>A0A1E3NQT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019046.1">
        <property type="nucleotide sequence ID" value="XM_019164054.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NQT7"/>
    <dbReference type="GeneID" id="30180741"/>
    <dbReference type="OrthoDB" id="2149224at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032065">
        <property type="term" value="P:maintenance of protein location in cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13365">
        <property type="entry name" value="PH_PLC_plant-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024774">
        <property type="entry name" value="PH_dom-Mcp5-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF1">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12814">
        <property type="entry name" value="Mcp5_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="2121"/>
            <end position="2234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2055"/>
            <end position="2075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2263"/>
            <end position="2295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="602"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="676"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="764"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="862"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="936"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1136"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1220"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1283"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1350"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1420"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1501"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1629"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1781"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2273"/>
            <end position="2295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2295" mass="260181" checksum="0E1471F6A3EEA9A6" modified="2017-01-18" version="1">MSQFSGNNSDIQDWDERPLSPVNITLGTNSSADLKSQLSQLQQKLNLNSIRPVRPSPNTEPEIHLPPLATLSSSQSNSLGNSTRTPDKSVDFLGDLSDGLLMESRKLSYENKQFKKQVSKLSAENESMKKQISNLNLLNKQLSEKEEQSNDKIWNLESELGNFKKSLDKTKSDLAKVKSDNTASISLIEQLKNSIENLTNERQSLLETSSLTISKLNSELSELKESNENLNDENDILHKNILDLKSEINVMKSEIAGSNTQSERLAESSFSEFDDSIVQDPLPAVLTEDLSKLDTDTLQKNLKLSYRQLLKLKSQNTRFKSELLKLKHGSPSPNKVQLLPKSLNDENDVENSWGYFEDDSFAKQPKKSNIVSSLIRSINEDDENEEDGDDDTNSFLADVSQLDGSSPVSTRKSSVSKPKESYILIVPKTTLDLNNNAINLKKIDLSEFQTIQVPDPIATELLASSSNKEISKSQFYNDEMLQLVSSHELSSLKSSIEEPSIDYVKSKALYHDHVSIPVKEHENLLSSTKQSAERIFELENLCRNQNNSLSSIKKQLEQTSTELTSKFGAFNSLKSEHENPSLEYINSKLPLHNLVSMDSQSHVEIKQRLEILQDKIKQSSENLDSLREIIEHLKNDKSNLEAQLRSPDIQYMKDKSTVMHYVFIPESEHIALKSDMTITEAKLKKKESEIRDLVDEKAKFEGKIADLEKIVTELKNSCSEFESKVLKLEEFQECPNSEYLRTKLSTHGLLALPVEEHIKLKTDNSLYNERIIKLTAENEAAKDENRRLWSDANERENKLKSVENKLDTVLKDFETIKQEKEQPGVEYIKSKASLYNLAVIPLEEHQSLKKEISTKDAKLADYAKLRLELDSKHDEIQQLINSKEKLNQLVKNTIASLKKRDSELTSLKQQLESPSYDYIKERSEAHGLAAITKEEHEKLKSELLESSELLAKKESEYASLQKIKDDLQREGTKSVSLDLHQSVQSELDYTKHDLNEYKRQVNNLKQVEIALKGKESELVAMGKKVSELETKAAQIDELNFELQNLRNAEEDLNKKQNELEGIKTERDEMQEKITSLQSKETQLLETLKNFECQNSELKSFHEKPDLDYIKEKSKFLGFLPVTLEEHTMFQSNLKKLEERNKKNVELEDNIVSLSTKNNELTELNNTLQKTVQELTEIKNKLQNPDIDFIKEKSSNLGLVSIPLSDHHNLKTKSETLASNIDALRSKISVVESEKAELNEKIKSLELMNASPSIEYIQSKSASLGIIPIPIAEHNSLKSELERNDILSKERDSQMKELNLLRKDITERKTQLRNAILQLNKISSSFEAPTMDYLKEKLKPFKYVAVSEADFESLKKSHEISVQQNENLEKKLVAANAEQTEKDNTISQLNRISSSYQAPAFEYLKEKLKALSYVAVPEADLETLEKSQEISVQKIEILERKLAVTETEILEKDNTISNLNKYALCYESPTVEYLKEKLKSFEYIALPESDLNGLKTLQNSSIEQIDNLKKKLAATNADLTEKDKQLEKLSVQLKAMINRDQSKNTNSKVVAAALPTRSSSFNIEGAQKGIEMNKTSSVVTVTSANVNDALSESIESIISRIEQNGYTVLTMEEYENLLKRGNNDADLDNLADITHEIEDIESDLESKKQELETKSRSSTISSSSSITSVSVENILSQKYSKLYDLINDLSIEVKNLKQEKARIVKQINRLSVSTNTLSNQKLNSTLSKKISRISDKIEIKEIEMKSQQSAIVAVKAYLEQSRGMDLPPVTAVTAEGDSDKDRSELEKEIALLQSKYDEKKQKMEKLVKEINNTDEPSQLVERLSLLGYTFTSPSGNTTMIREISINDNVIYLPAIIQSGSFDPSSGKLSVDKLAQENGFALISAAEAKQKQNIFDITDLSINDLGARAKELKHTLISDQKLKLIVAKSNEPKLPESLTLEELEPFAKKLGLRIISNEDIARLKARNITTRELASKAEELNLVLLSQDEVNDLNTHEPITEKNIVKKGKELGYLCIPSSQFVATTVSRTADIPNVTVLPNSYYKILTKSHEWYKKNKDKLDQSPAKQPQTIPEDDSFDLPSIMPNATPSGHQLPVSNIDTVSLHTVDTVISNKKLIIAAVTQTIIGEYLYKYYRKLGPFTSVSDTRHERFFWVHPYSMTLYWSPANPVVSDPARNQIKAMSILDVQCVNDNNPLPPGIHYKSIIIKSHNKSIKITCPTRQIHNIWYNSLKYLLERSMDSWVNDDDLEDQYQHDFTLDSKTKLERTQSQKFRRSHLSASDNRLTSKSTSLRSMTGSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQV6</accession>
    <name>A0A1E3NQV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14012</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019549.1">
        <property type="nucleotide sequence ID" value="XM_019160183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQV6"/>
    <dbReference type="STRING" id="763406.A0A1E3NQV6"/>
    <dbReference type="GeneID" id="30176870"/>
    <dbReference type="OrthoDB" id="185618at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053074">
        <property type="entry name" value="NPC_Nucleoporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015007">
        <property type="entry name" value="NUP2/50/61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38697">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SIMILAR TO S. CEREVISIAE NUP2 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38697:SF1">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SIMILAR TO S. CEREVISIAE NUP2 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08911">
        <property type="entry name" value="NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RanBD1" evidence="9">
        <location>
            <begin position="489"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="330"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="447"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="468"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="613" mass="65152" checksum="4D1FDDB101E253F7" modified="2017-01-18" version="1">MAKRTKTQLTKDAFVAYGDNAENSGDDDQEMDPPQHASEAVMAGRKIAGRGRRFGAKKPQVNEASAAISSAATDKVQLSQMRSLNANFLKSVNDGITKNPVADFTAICTKYLDYVSKVQDKKIEVKKMDVPKVDVKPESVKTEGKANPFAMFAPVNNNGNDPAVAKPNFGVDTKLGTPVENAQAPARPAITEAAKVDEGSDDDEEDEKTTKPITLEGPKFVLSELPKSKSYGFQFGKAPPKDEDDSEDEIEIKGPSFVSTATVSDSHFKFPPKADDKKDSKLGDAAPVAKPSIGFSFGNAASTSAKPAFSFNLPASSDKPAAETNKVSFNFGAASKPENDTKPAFSFGAAKSSTPSFSFGATTLPTTTTGNNTTAPVLNFGQKATPSSAFSFGSSSATTTESKPNTNTNSVFSFGKNADASSNSSNPFNIGKTTAFGNSTSPVFNFSANTPSSSTSNEPSVTAKKTEDDENPDEVNEAESDTVKGNFAVVHLTEKVEVKTGEEEEESLYAKRSKITKFNFETKGYDPVGLGELKVLKNKATGKCRILVRSEGSSNVLLNVAILEGMKYELLGDKKSMLRIPSVNADGTLVTYVARVKTATDGEELLKAVQSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR23</accession>
    <name>A0A1E3NR23_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019637.1">
        <property type="nucleotide sequence ID" value="XM_019163571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR23"/>
    <dbReference type="GeneID" id="30180258"/>
    <dbReference type="OrthoDB" id="10350956at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="290" mass="32572" checksum="36F306127A3BFE75" modified="2017-01-18" version="1">MLLNNSRYTSDRAQFAERVGRIGNVGEVLDLPYPQIAGFKSRNPNTARKPAVHSNTSREGGNGDGKGTGDRDCVYANTNNPRFTMSNMSSQNTLISQQTNSTVATTSSESEELFPIEKLEELEANMVNYMHNPRRNSITSSIRIGKHFDNINASKYSIHSCKKTSVTNVSKNNIVLINNMPYPVQLQFPAKISNRKSKEIAMRPDDMGIENKENMNRNYKIIRKDNTSNSASLGSNYKGNSYIEEVYFEPSPSNNSSENLTINSSDNNIIDPRFNDSYDLLLQLIQSYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR64</accession>
    <name>A0A1E3NR64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_14157</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019730.1">
        <property type="nucleotide sequence ID" value="XM_019160249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR64"/>
    <dbReference type="STRING" id="763406.A0A1E3NR64"/>
    <dbReference type="GeneID" id="30176936"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110004">
        <property type="term" value="P:positive regulation of tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.40:FF:000003">
        <property type="entry name" value="Serine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="155"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="281"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="297"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="368"/>
            <end position="371"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="13">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ48617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="460" mass="52572" checksum="13F7BEE594971FA4" modified="2017-01-18" version="1">MLDILQFIEEKGGNPQAIKESQKKRGASVELVDEIISEYKEWVRLRFVADELNKKLNKVQKEIGLKFKAKEDASELLKEKDTIIAEKKEAIDKEQEADKQLRQKVYLVGNIVHESVVVSQDEENNELVKTWTPEGLEFEKLTDVATATGKPAKLSHHEVLLRLDGYDPERGVKIVGHRGYFLRQYGVFLNQALINYGLSFLASKGYTPLQSPVMMNKDVMAKTAQLSEFDEELYKVTDGDDEKYLIATSEQPISAYHSNEWFESPAEQLPVQYAGYSSCFRREAGSHGKDAWGIFRVHAFEKIEQFCITEPDKSWEMFDTMIANSEAFYQSLGIPYRVVGIVSGELNNAAAKKYDLEAWFPFQKEFKELVSCSNCTDYQSRNLEIRCGVKTQNQKEKKYVHCLNSTLCATERALCCILENYQTEDGLVIPEVLRKYIPGEPEFIPFVKALPKNSTSNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR96</accession>
    <name>A0A1E3NR96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-arabinitol 2-dehydrogenase [ribulose-forming]</fullName>
            <ecNumber evidence="5">1.1.1.250</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; D-arabinitol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.250" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019196.1">
        <property type="nucleotide sequence ID" value="XM_019164087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR96"/>
    <dbReference type="STRING" id="763406.A0A1E3NR96"/>
    <dbReference type="GeneID" id="30180774"/>
    <dbReference type="OrthoDB" id="5325318at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047038">
        <property type="term" value="F:D-arabinitol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000240">
        <property type="entry name" value="SDR family oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF14">
        <property type="entry name" value="DEHYDROGENASE ARBD, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="274" mass="29627" checksum="CDAD6721A54E5176" modified="2017-01-18" version="1">MATGVPNFKLTNELTIITGACGGLSQALLHTLLAYEAPVALFDRNMEALKKTKEELVLFCTAELGVKADAVPQMECFACNIADAQEVEAACEAVYTAFGTYPLHLVNTAGYCENYPAEEYPAANAENLVKVNLLGSLYIAQSFARPLIKHGIRGGSIVMIGSMSGVIVNDPQPQIAYNMAKAGVIHMVKSLAAEWAKYNIRVNTLSPGYIATPLTKNVINGNADLYNRWMSMVPMKRMSDPSEFSGTVLYLLSNSASSYTTGENIVVDGGYQCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRI1</accession>
    <name>A0A1E3NRI1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_85666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019803.1">
        <property type="nucleotide sequence ID" value="XM_019165018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRI1"/>
    <dbReference type="GeneID" id="30181705"/>
    <dbReference type="OrthoDB" id="10488058at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="899" mass="103870" checksum="194F5A1CA7250118" modified="2017-01-18" version="1">MSRLVKRTLQSQLWCGEKQIIEAFEGFIIYNEKSIVYFDSMPGGKELNCKFKKTMDEQIVTVTFLPYLQVIAILFFNREGNFYCQTYDVDFNKSPITTKSSNAPPREDYLSHGLCGKSHIYVYSYFKHELLFTILPIDEFIGNSKITLSGFNVYLLGKNEQMKACAKRYILTYTSTWCLTETKLWSDVAESFNVFDCDVLACSQKYLVATLFNSIYVLDLNKKGKKTTIETDSIITSFSWITNSKAFLTTDSYQLFFINMNTMKLQPIPSTSLSIGVEDVIVTKSHIFLHNKLTGFYVFDIENSSKFGFIRTTKYIPAQEINLFVGVSHAYRTNSSENIISFTDGYNSILTKKMLDINILQDRFRFIKNQLFIGFYQGDDFLTKDLNSQLYLNGKVAFSFTDEANLESITPSNALSMTGISEIAESVKESPTLFNHKPVHASCFQVYNNFTILYDDKRKSMHIFPNNKTFYLKTEFTPKSIRIYNCDTVSVTFLLFDHQHINVYTQTQSCEPVLISHVDWGIAEPILDIDFSGSFDSLYVRTLLENVWQVKIPEVSSILISERLYFSHIFSLRHRLIGISSLENKIYCINYESCKINKLIRTFEGKLLQCSKISNSSIIVSTTTEMFEIELEVGDERYYESALLGVTKASLNYDETQKLVHTSNGLYMVDTIHLRVHASILLPHINDVKVDKNIDRIIISVWNPNETKTAIILKVSYNKELGKFEIESQALKMNVVGKVLIEPTCSQVSFSGELYSYKDSDNFSSAIGTCYQPPAKDWLYVEKQNYYSYLTETKYFVVSDQSDGNLIWFDNVNQYFALNNSNFLIYKDDSLYFFDFQEFQLNRLCKLERGFHVGKDLKIQWIDTSLLTWDRSRCLWRSKPGYFITAINPSGLYEEYIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRL7</accession>
    <name>A0A1E3NRL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease</fullName>
            <ecNumber evidence="9">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_24415</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase HII family. Eukaryotic subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019880.1">
        <property type="nucleotide sequence ID" value="XM_019162351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRL7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRL7"/>
    <dbReference type="GeneID" id="30179038"/>
    <dbReference type="OrthoDB" id="7462577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990516">
        <property type="term" value="P:ribonucleotide excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07181">
        <property type="entry name" value="RNase_HII_eukaryota_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF7">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="11">
        <location>
            <begin position="34"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="290" mass="31972" checksum="25E56EBCBDA0DCAE" modified="2017-01-18" version="1" fragment="single">PPTVPLPTPIHTYTNHSPVPSSLQAKRVDGSPPSIIIGIDEAGRGPIIGPMVYSLAYCSSEFGAGVLPNCEFADSKKLTEPKRWELMKRVCTESVGDGEDENALSNNLGYAITSLTALDISSSMLRPSSLVVNLNEQAHTATIELIKAVIQRLREEVCENVIIAGVYIDTVGPPASYVKKLRQFFSVDDVKEIRVEKKADATYPIVSAASVVAKVTRDWFLECAAKETGCAEGYENWGSGYPSDPRTSSWINGKLDPWFGWDLNIRYSWGTARDAFNRSKVSVEMRWEHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRU7</accession>
    <name>A0A1E3NRU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_87240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019923.1">
        <property type="nucleotide sequence ID" value="XM_019165027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRU7"/>
    <dbReference type="GeneID" id="30181714"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133489" evidence="1">
        <location>
            <begin position="37"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="155" mass="16882" checksum="C98AC50F475F0502" modified="2017-01-18" version="1">MTIPIAHTMIHGARTQIKKMQVLLAFLTQSVGGCFGDQITDDGGESYDTEPAHCDRDLLAGPGQEPLQPKFSFFDVVSRSSCSTHSDFSFKNDKLALGLSRSDANSGLPDIYELRTDAARASEVPCSLFPAIDYSQFNPSDPNYIPLFPCSMVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS92</accession>
    <name>A0A1E3NS92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_83638</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019661.1">
        <property type="nucleotide sequence ID" value="XM_019164999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS92"/>
    <dbReference type="STRING" id="763406.A0A1E3NS92"/>
    <dbReference type="GeneID" id="30181686"/>
    <dbReference type="OrthoDB" id="28208at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133480" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="56"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="718" mass="78068" checksum="2EBBBADC432F63F2" modified="2017-01-18" version="1">MLLNPIVLSALLNAVNAFPLYKRDSNSSVAAANSTAEDKYLSFNFIHQDLSGAYLPTIQVAIGSSNQTLLLKLDTTTSDFWVNANTNEFCLAYYPIEDYLNETNPNDDWNDFNSELEAEFEDAFSDFEGAENSQFSTVETASANIESYLSERQSAVQSFLSQQKASVSQQIETYLTTATATGDDAWSTFEAGLPSQVNEIGATITSQTAAQALPFSGNPSLVTSYSQYSTASFTYVQVTGGYDENGPFDGSSDYPVASTLASWELLQKREDGWEKFTSGADKFFDDLKKRDEKTTTTKHHKSSASHKSKASSDVSQASKTTGVASFASKASRVSHHASSYTSTHASSYVYAPSPVLLSAYASASALATPSVDYETLEYIHELYNDCGLYGVFNESASTSFQSSDKLFVSSDESSYGILGSDSVSINNYSVNTTFGVSELSDSNVGVFGLGKTTENSTFVSFTDVLAQSGYILKSLYSFTIGLPSEITFGAINYSYLTYSNLTLFPLLNDTESIGLTLSGISLLAYDEDSSNNTIPVVQGKAQAFIDTTSQNTYFPQEVLDTLATSLNSTLNVTYVDSIESYVIYDNSSYSSLWDYEIVFDFQGAEYSLPLAEFAVTLNGTGPSNDTDSSDSFEFYSETDVYILSILPSDDDSIVLGLDFLTDNFLVVVDLEANEIGLAYSILGEEDEFIVIEDDIPGAIVAPYYDDFYGSDNVSKLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSC8</accession>
    <name>A0A1E3NSC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TAFII28-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_178047</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020148.1">
        <property type="nucleotide sequence ID" value="XM_019161752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSC8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSC8"/>
    <dbReference type="GeneID" id="30178439"/>
    <dbReference type="OrthoDB" id="28335at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="7">
        <location>
            <begin position="276"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="458" mass="51079" checksum="512EE3004D87246D" modified="2017-01-18" version="1">MFCGHHKPLVRPEGLKFIAGMNNKAVKHGNDTLVDGKEVTKKQKVTSELDSVLNTSDLDAFLKDVSVPLVSSTEGTPDLDDMLLGGLPQPNLKEDVAVDRATVLKETNVAKSIKENNIPRTSSTGATVEGGNVYAMNANLSKPKPKKKNYNFTTGKNDGPQATDESGVPLDIVKGREYLHERFDTLRKVNMSELLLQKKREEDEGIQMDEESESESESESDSDSELGTGSDSGEESEEDKEEAEKVPKDFEDLLELFDINPDEMSLDLTINEKRKLLIDSMDDKQLNRYEFFRRTNLNIGGIKKLINSICGVSVPNDFAKLLGGVGKVFVGDIVEMAKQVQREENEARVGQQIRYKLGLRDFEDALKKYHAGTVDQLPRRPQIPPFYETLLQLDTGASLVNIRRRVDTYNNFRVVIPKEDAQLTPDHVREAWRRLRLSGGGSVEPWRRGAGGRGGGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSQ5</accession>
    <name>A0A1E3NSQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_70616</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020149.1">
        <property type="nucleotide sequence ID" value="XM_019163688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSQ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSQ5"/>
    <dbReference type="GeneID" id="30180375"/>
    <dbReference type="OrthoDB" id="1108038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16702">
        <property type="entry name" value="RING_CH-C4HC3_MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000287">
        <property type="entry name" value="RING finger membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="417"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="662"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="704"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="866"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1021"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1049"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1110"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1152"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1154"/>
            <end position="1163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ49036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1178" mass="136162" checksum="DA844718A3B0AD74" modified="2017-01-18" version="1">MVEEKFCRVCRCEGTPDQPLFHPCKCRGSIKYIHQDCLQYWLEHSNKDICDICHSKFKFKIIYNDGVPDSVPLKIILKQFFRDLIHYQYQVVKYSLMIFCAVFEVPTVISLIDKIIDFQLGVPIRIESPFLPNMFEDYKSSEYFIVNYLSFLQCVIFHGLFIAMTFSVIIISMIIIQNSFVGDEGFQKIIDKKIGPQRRKLVDLFQLNRRRRALRGQENEQRVNRELESVGWVEGHVFERAEILLMVNSLHRMIEELVEDPTVRDTVADQVYLRGLDPEVINDEDIQKVFELYKKYRITLLNHRTEEREMFQVIQNVANRDGRANVDDELVRANARLLMNFQDPDMAVPAQNIDDINEIDPPIPAPVVEQDDGQEQLQGMWDGPKNITFIFQLTLLANLVSMVVLVCFKLIPSYQGMLFLSILDALVFSPLQKLFMIAHPYFYPYISLLIKSSPAQYVYAQSQNFIVPKLILAFFQRNLILPLRAAYVNSVQWTPTSSSSERIIVTLTGFAVFGLIIFACMKKMERSCTPSNPLAGNYRTIYIVMLQIASIVKVFSLIAIEWAIFPLFCGMQIEFALVPIFNDNLYNYKLDPPFFGTDFFGVVPKWFMGTYFMYFFASFVSMIRSNILRDGVLFFIRPSDDPNLQLVHDALMRPFSLRLSRISLSAAVYSLYIHIEFSAISWGIRLFSPIKILPFHNEFFHERALFVLVFILGMSLEKSFCKIWKAVFKFACSQLRLSSFLLDEDNPKERGKIIYKTLFAKLTNPTPDYSEPVLESETDLYFATHTSALCCFVPDGNYVRAPNDDHVSRNFVRTLFVPVTKSDQLLAPIPEYPDDDEIYNPYGDVDPMDVTTYTIVYRPPDFKWRLFALFGILWLASMLITLALYVCNILIGEPLIKFVVPKRITGALKIPTEWYKVDIYGVIITLLALPRLEKDTKAVRNQWSALIKAYTDSKSNGERVKAIKIIIVDNINKLREHYRIIGNNPFVKTVVKQLHQQFILAIGLSHCGSPVLEYRLKTNRFPLWSLLYFASYIPLALAFWGQNKKIVRLYFFTAVCVLCAKTSFTCFQIFTASAEIPNHKPKTESSYTYPSLPGFLLGSFFSETRDVESWIYFSSWILISSYTSYVYIVSGWRKFKDKTKQMYFDNMKVLSNADDGDNEDSGESNEGTLDESRADNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSZ2</accession>
    <name>A0A1E3NSZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">40S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14688</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020334.1">
        <property type="nucleotide sequence ID" value="XM_019160485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSZ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSZ2"/>
    <dbReference type="GeneID" id="30177172"/>
    <dbReference type="OrthoDB" id="1702480at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17114" checksum="6D9F4AACD0BAB09F" modified="2017-01-18" version="1">MGLVVHESGSFQHILRLLNTNVDGRIKVMYALTTIRGVGRRYANLVCKKADVDLSKRAGELTQEELERIVTIMQNPTQYKIPAWFLNRQKDVVDGKDHHVLANNLESKLRDDLERLKKMRAHRGLRHSWHLRVRGQHTKTTGRKGKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT09</accession>
    <name>A0A1E3NT09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_57264</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020297.1">
        <property type="nucleotide sequence ID" value="XM_019163226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT09"/>
    <dbReference type="STRING" id="763406.A0A1E3NT09"/>
    <dbReference type="GeneID" id="30179913"/>
    <dbReference type="OrthoDB" id="1699231at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000005">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000011">
        <property type="entry name" value="Vacuolar calcium ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF48">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="18">
        <location>
            <begin position="61"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="18">
        <location>
            <begin position="303"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="19">
        <location>
            <begin position="454"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ49184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="750" mass="81006" checksum="2A5ACEA997ADA16D" modified="2017-01-18" version="1">MSSENTALLEQESSPQQQPAQSMLKSGYEACATTLRSSPVNWLLLFVPLGIVAEKVGMGAVTVFTTNFMAIIPLASLLAFATEELGESIHNDSIAGLLNATFGNAVEVIVSVIALRQNQVTVVQASMLGSILSNLLLVLGSCFIVGGLRYKEQFFNQTAAQTMGSLLAVSVMALLLPAAFYLSLPKDTSHLADKILNFSRGTSFVLLTVYVFYMVFQLKTHAFLFTEDADASAENVIQDLEAQPANPAQALESAEPASYYGQENTAPESASSHSRSNSFLINRPKSIYDLGSSPEQHLSAISAIAVLLLSTVMVSISADYLVGTIDEIVQTSGLSKTFIGLIIIPIVGNAAEHVTAIYVAYHNKMDLAIGVAIGSSLQIAIFVTPFLVLVGWIMDVPMSLYFSMYETAVIFVSVFITNSLILDGSSNWLEGVMLVATYFIIGISFFLFPDTLGELGLPLTPVEFKRDSNKEVLFSIGESIRDEDIYIIMQIGAGPVNDKVFELLIMLNACRAASARRITAVLPNFPYARQDRKDKSRAPITAKLMADMLTTAGCDHVITMDLHAAQIQGFFDSPVDNLYAEPSVVRYIKEKIDMKNAIIISPDAGGAKRAAGLADKLDLNFALIHKERARANEVSRMVLVGDVTDKICIIVDDMADTCGTLAKAADVLLDNGAKSVIAIVTHGILSGKAIQNINNSRLEKVVCTNTVPFESKLSQCPKLDSLDISGVLAEAIRRLHNGESISFLFTNAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT76</accession>
    <name>A0A1E3NT76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 8">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_29922</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="3 8"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019981.1">
        <property type="nucleotide sequence ID" value="XM_019162611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT76"/>
    <dbReference type="STRING" id="763406.A0A1E3NT76"/>
    <dbReference type="GeneID" id="30179298"/>
    <dbReference type="OrthoDB" id="193380at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006406">
        <property type="entry name" value="Nic_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01514">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 8" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="10">
        <location>
            <begin position="11"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="168"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="430" mass="48806" checksum="F6690D32BD817E3C" modified="2017-01-18" version="1">MAVTPIITSLLDTDLYKITMQAAIHKHYPKVPCEFLLTNRTPNKKLNRKAYDWLCQQIKSLESLRFTNEEIAYLKTKIGYLGDDHFEWLRTVSLNPDKEIKIVPEISGDEYNLTITACGDWEVVTLYEIPILSLLSECYFRFIDKKWKIEGKEVAILENEKLLEAGCTFSEFGTRRRRSFEVQRTVMQGIMKAAKNSDFKGKFLGTSNVFFAKEFDVNPIGTIGHEWMMGVGAIQSMITSTVDGEPNYEAYLTANRLAMEQYVELVGPSHAGLALTDTYGTENFLKQFTEPYIGYYVGVRQDSGDPKEYARELSAWYHNHGYSGDKRKLICFSDSLNVSKCIDLKKVCDSQECAINSIFGIGTNLTNDFDTEPMNIVMKLLTCGGGHAIKISDNAGKNMGDSNTVHEVKKRLGYVEHKWAGGDESHRWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTQ3</accession>
    <name>A0A1E3NTQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase subunit 4, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome c oxidase polypeptide IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_57166</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 5B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020161.1">
        <property type="nucleotide sequence ID" value="XM_019163219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTQ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTQ3"/>
    <dbReference type="GeneID" id="30179906"/>
    <dbReference type="OrthoDB" id="10249250at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00924">
        <property type="entry name" value="Cyt_c_Oxidase_Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.11.10:FF:000003">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002124">
        <property type="entry name" value="Cyt_c_oxidase_su5b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01215">
        <property type="entry name" value="COX5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00848">
        <property type="entry name" value="COX5B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51359">
        <property type="entry name" value="COX5B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="152" mass="16908" checksum="808393B4EECA7C01" modified="2017-01-18" version="1">MFRQTLRVARSTRLFSTTSLARNNAPKEFKTAKTLAEVETKFDLIGKGAQPGEVPTDLEQATGLERFELLGKLEGIEVFETEPLDSSRKGTMANPVMIDSYDDYRYVGCTGSPAGAHVVMWLKPTKDKVARCWECGSVYKLNYLGTGEDSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTV6</accession>
    <name>A0A1E3NTV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine transaminase</fullName>
            <ecNumber evidence="4">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15007</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020688.1">
        <property type="nucleotide sequence ID" value="XM_019160631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTV6"/>
    <dbReference type="STRING" id="763406.A0A1E3NTV6"/>
    <dbReference type="GeneID" id="30177318"/>
    <dbReference type="OrthoDB" id="1703350at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000010">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="13">
        <location>
            <begin position="11"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="389" mass="42747" checksum="643B893AA2579744" modified="2017-01-18" version="1">MCIDREEPNYFGAGPALLPTSVLQQAGKDLINYNNLGIGAGEISHRSKAGVGIIEQTKASLKELLSIPDTHEVFFLQGGGTTGFSSIVTNLTAAHVKKHHKAGTPYYVIDGTWSKKSFEEAERLGFNPKVIVNSKEIDGKFGTIPPTEKWNIPKDLSDASFIYYCDNETVNGVEFRTFPFEKFPGVEIAADMSSNFLSKPVDVSKYGLIFAGAQKNVGVSGISIYIIKKSLLDQAPAEELRKLGLQVTPIAFDYPTVVKNNSAYNTIPTFTVHIVNLVLKDLIAKGGLAAQEKENIAKSDLLYSTLDKYPSIYKLPVEKSVRSRMNIVFTIEGSGLEDQFLKEAEQSKLEGLKGHRSVGGMRASLYNAVTLKSTKLLCELIERFAKEHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUZ1</accession>
    <name>A0A1E3NUZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14831</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020476.1">
        <property type="nucleotide sequence ID" value="XM_019160557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NUZ1"/>
    <dbReference type="GeneID" id="30177244"/>
    <dbReference type="OrthoDB" id="431929at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057358">
        <property type="entry name" value="UBL_ZFAND1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF40">
        <property type="entry name" value="CDC48-ASSOCIATED UBIQUITIN-LIKE_ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25327">
        <property type="entry name" value="UBL_ZFAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="6">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="290" mass="32852" checksum="E31A6390B6984169" modified="2017-01-18" version="1">MTTVGRKNEDGMMDIGKHCTICRQLDFLPFVCPKCEKSYCNNHRMDFNKHQCVIDEQKAKLAALERAKVDVSKLPKSADVFPDLDKIRRDAEIKHKEERNKKIGQRLTDVKDSNNKPLTSIEVAMLRLKKLLGNSAKSKKSSSTKPSSGSSLLRGFVGGSSKPDSTTSRMVEMNKLKRSAKGDNRINASDRVYVWVIYTSDDDTTFEVKSGQFFSKKWPIGKMLDSSAEISKIKNVNNREVDESLRLAMFRKVRSGEKDAASEFVYIPTSGRVEKEIRDGDEVYILRGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND71</accession>
    <name>A0A1E3ND71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_74913</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015188.1">
        <property type="nucleotide sequence ID" value="XM_019164910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND71"/>
    <dbReference type="STRING" id="763406.A0A1E3ND71"/>
    <dbReference type="GeneID" id="30181597"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF15">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23654" checksum="221A9955A3454DE4" modified="2017-01-18" version="1">MIKIKDKATQRKYAQTKFPTWTYKVRDGALGGLYGFPCTEGYMKIGYRGLKWINPVGDINSKVKTKWSPEEQETNIPLFALKQIKNFIRENIPEVKKISMTRLCWYSDSSDNDYLISYCPYYEDRSVFVMAGDSGHAFMMLGSIGDVATDIINDEGDPFLRNLFSWERERETLNIINSGAEDPRCLTETVMASPKDWWIDEPSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDA2</accession>
    <name>A0A1E3NDA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_18561</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015213.1">
        <property type="nucleotide sequence ID" value="XM_019162060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDA2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDA2"/>
    <dbReference type="GeneID" id="30178747"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="398" mass="44254" checksum="4AC6DFDDF72594C7" modified="2017-01-18" version="1">MTATLANSNLFKPIKVGPVELKNRLVHAPTTRFRNTDEFVATDSMLDYYTERATDNGGLLIVEATFPSPEFGLFERAPMIKTPQQVKAWRRITDSVHAQGSSITLQLWNLGRVADPALLQKHNLPFVGPSAIYLDEASEKAAKEAGNELRAITVPEIKAMVAEYAASAKRSIDEAGFDMIEIHAANMYLPDQFLQETSNTRTDEYGGSLENRARFILETVDACIAAVGAERVAIRFSPYSEFQGSLGAHAETNPIVTWGYVLSELEKRAKHGNRLAYISIVEPRVSGTDEHPDTDPVDFSWPDHIWRGILLRSGGYLSKDAIARLPDVVNSNNHTLIGAGRYFSSNPDLVNRVRNTLEMTQYERSTFYTVGNVGYLDFRESGQPLDHSKDHLVPKPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDG0</accession>
    <name>A0A1E3NDG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mug135-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18423</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0612 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015270.1">
        <property type="nucleotide sequence ID" value="XM_019161993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDG0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDG0"/>
    <dbReference type="GeneID" id="30178680"/>
    <dbReference type="OrthoDB" id="10310784at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013902">
        <property type="entry name" value="Mug135-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08593">
        <property type="entry name" value="Mug135_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mug135-like C-terminal" evidence="3">
        <location>
            <begin position="149"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="234" mass="26805" checksum="8CB515D8C6B922DC" modified="2017-01-18" version="1">MDSLELQQQSGAVDEPQNPLDEELDIPDDVFVNQENVALPQPKTRANIMQFEQELSEKAVIANDEVYRARKRVDRADVTKYKVQKALAQTNNENSLIALIRRISNDIGSINRNINTMQTNINTMQTDINSIKDEVSGMKPLMLYVRTSENARRRELREPSIPVPFLVGEGPEGTDLPSINSVEDIELLDLEQLRRFLTGYNVRYALRTSRVNMKIMLRDTLGFCRVSDMRMNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDT0</accession>
    <name>A0A1E3NDT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015393.1">
        <property type="nucleotide sequence ID" value="XM_019162041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDT0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDT0"/>
    <dbReference type="GeneID" id="30178728"/>
    <dbReference type="OrthoDB" id="4092852at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="73" mass="8171" checksum="71DBC2721DB43ED2" modified="2017-01-18" version="1">MVRFSEASEHLMDFNIRSMSEFGVYPLVIEKSVTLVALYVDDLLILLNVSSSITFVKDVLSSHFRMKDLGAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDY2</accession>
    <name>A0A1E3NDY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="6">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_37404</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015458.1">
        <property type="nucleotide sequence ID" value="XM_019163084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDY2"/>
    <dbReference type="GeneID" id="30179771"/>
    <dbReference type="OrthoDB" id="1664372at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="9">
        <location>
            <begin position="23"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6 7">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="36"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="319" mass="33699" checksum="6EA684707295F168" modified="2017-01-18" version="1">MFRRSFHTSRLAKNYADTIKNLKVTKDTKVIFQGFTGRQATFHAQQALDYGTQIVGGTNPKKAGEVHLGKPVFATVAEAMKETGATASGIFVPPPIAASAVAEAIEAEIPLVVCITEGIPQQDMLRISQMLKTQSKTRMVGPNCPGIIAPEQCKIGIMPQSIHKRGRIGIVSRSGTLTYEAVAQTTQVGLGQSLVIGMGGDPSPGTNFIDALTLFVNDPETEGIIMIGEIGGSAEEEAAEYLKQFNLTRDVPKPVVSFIAGVTAPPGRRMGHAGALISGGKGTAQSKIETLSAASVEVVPSPGRLGAALYQQFVDRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE30</accession>
    <name>A0A1E3NE30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_179251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015504.1">
        <property type="nucleotide sequence ID" value="XM_019161807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE30"/>
    <dbReference type="GeneID" id="30178494"/>
    <dbReference type="OrthoDB" id="10563459at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="212" mass="23684" checksum="F790C5435BA40299" modified="2017-01-18" version="1" fragment="single">GLKGSRCGRHKHALLNSNIHCGVNNWSCQLEYRGMGRKGNQIPAVVVRADDFAVDVMQNSDEGREVSGDKLERVTWTRDNIAILREVLPLLEKTTEGIVVRYNFTHLSNILKIDRKHIYGKMIELYRGDDENMPEAEVREVLVKSAMDPRADSEPVSVDSLINVAAASKLLHSRRLLGQPSGDESSVGESDESDESSVEESLLQREILESDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE71</accession>
    <name>A0A1E3NE71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_60555</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the stereospecific oxidation of D-lactate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015544.1">
        <property type="nucleotide sequence ID" value="XM_019163444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE71"/>
    <dbReference type="STRING" id="763406.A0A1E3NE71"/>
    <dbReference type="GeneID" id="30180131"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000038">
        <property type="entry name" value="D-lactate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="14">
        <location>
            <begin position="162"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="597" mass="65337" checksum="71F6E7F71E066931" modified="2017-01-18" version="1">MARSKLLSLWQLSRNAGVLGAGTRRFATSIPRGNLAKPGQLKKEPEPEPATSNGKGGEKKENGDKEKDGFGQSILANTAILVLGALVGTSWASFNLCKEPPEFLFPHTSTLPLASTLSPLYGDSKLVIEEVKGFLNEDQVSESKDEIDNHSDSFFSTDHPTPEEHPDAVIYPETTEQVSQIMQAAHKHHVPIVPFTGGTSLEGHYISTRRGICLDMSRMNKIVALHKEDLDIIVQPGVGWEDLRDYLQPLGLIFGPDPGPGAAIGGMCGTSCSGTNAARYGTMRENVVSLTVVLADGTIVKTRRRPRKTAAGYNLTNLFVGSEGTLGIVTEATLKLNVLPKFENVAVVSFPSMSDAADAVAKVVQSGVQFNAMELLDAQMMKFVNVSGNVTKKYDEKPTLMFKLGGNSDKVVNSITNDIKDICNECNCGKKNFRFASSDEEKEELWTARKVALWSTIDAGREVYGEDAQVWTTDVAVPISKLVQSLVDTRKILDDEKMMSSIVSHAGDGNYHTFIVYPKKDHDRVRQVVQHMVTRALELDGTVTGEHGVGIGKREYLREELGDVGVNVMRKLKFALDPLLILNPDKVIQIDPEEKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEF1</accession>
    <name>A0A1E3NEF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015628.1">
        <property type="nucleotide sequence ID" value="XM_019161986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEF1"/>
    <dbReference type="GeneID" id="30178673"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="104" mass="11527" checksum="C69EE808467B9949" modified="2017-01-18" version="1">MLLVYQYITNTAQCCCLPPRYRQQHIFTSTITTATHLHYYNNNNNRPHFFLSAPAPTAHSCRPHCLATQTSPPRVANGSVATHYSPHFSSEAADAPIDASLGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEM5</accession>
    <name>A0A1E3NEM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_173614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015694.1">
        <property type="nucleotide sequence ID" value="XM_019161565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEM5"/>
    <dbReference type="GeneID" id="30178252"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="85" mass="9180" checksum="77B7C53E639E175F" modified="2017-01-18" version="1">MAELHPGDPVRHPQPCSFPRPGSILVARWPTIRPALSCLTHPPHPLPPALSSHPLFARAFAANPAPLTGRQCFQGNGATSPHRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEN1</accession>
    <name>A0A1E3NEN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_36559</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015697.1">
        <property type="nucleotide sequence ID" value="XM_019163017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NEN1"/>
    <dbReference type="GeneID" id="30179704"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="247"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="166"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="564"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="581"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="673" mass="74931" checksum="76227013A1E15CB7" modified="2017-01-18" version="1">MPRLGFSSLTHKFHDEHHGASNHNPGNINNSHSSNSITSHASSSNVSHDAHHNLLNFSSPSSENIHHIHNASSSPMNHAPSSRRSFFGLNLGKSPSNESQYSIMSDDNHHVHNNQTSAAPANHHNNNSSSNLTSTASSSHVAAPPIQRNGSSSMVQLKKFFRPARKNSNDHIHSPLNHSNSGLNNRRSPHGHSNSNLKSLSQSQNSSPAPQTPSTPTFTHDANNNMIVKTDQKFWDDLEGSVTKKYGKLGKVLGSGAGGSVRLIVRESDGVTFAVKEFVPRRPNESVKEYAKKCTAEFCIGSTLHHPNIIQTLDIINENNHFYEVMEYAPIDFFAVVMSGQMTRSEINCCLKQITLGVSYLHSVGLAHRDLKLDNCVVTKDGIVKLIDFGSAVVFKYPFEEETVKAHGIVGSDPYLAPEVLTSTDNYDPQYVDIWSVAVIYCCMTLRRFPWKAPKKDDPSFSLYCMEDDQPHDYVESARKHKELLIERKKKIVQLQKEKEEKGTQPQAPPQTEDPVQSVTEKVNDLNVETQPAPPAQQSTQQENPMTLQDEILLGDPDQVAVKPKVGSQMDEEEPTTSDKPVAKFDDKGNEVPERRTAGATKSQKQIHGPYRLMRLLPHAARPVISKMLAVKIEDRATMKDILDDEWFKEIKYCTIDEDGKTINDPSHHHTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEP7</accession>
    <name>A0A1E3NEP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015679.1">
        <property type="nucleotide sequence ID" value="XM_019161839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEP7"/>
    <dbReference type="STRING" id="763406.A0A1E3NEP7"/>
    <dbReference type="GeneID" id="30178526"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="361" mass="41294" checksum="3EB06F3FE5F0E86C" modified="2017-01-18" version="1">MSSQKDALLDQILSSITDSIDSTSTVVEKLEEEFSLKNKDEYPEVVRNLIKSGDSTTSNALNPVSLLSLKNSALLGYVTDLALLLAIRLESIKEEKNTSNNEENVLNSIVTNRVVLEKGIKSLEKKINYQLEKMVNAFHRREKEQSDLEHKVKEQQEQDSSKEENEEEEEEEEEEDEGLGFKPNPSALLSRGAEKGRTPTKDDIVEEAAEDDDQGEKKVGKYKPPKISAAAINDPDKQLKNRKQRNLQSMDEYLQDISEAPATEKSIGSTIINKGRDVKSRKQMEKEAEIQRYEEENFTRLPATKTKEDKRERAKRMRNEFFGEDWSMFDNNMDINSNGNGSTKKRKRVSAWERAKRKIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NER0</accession>
    <name>A0A1E3NER0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_173826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015722.1">
        <property type="nucleotide sequence ID" value="XM_019161578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NER0"/>
    <dbReference type="GeneID" id="30178265"/>
    <dbReference type="OrthoDB" id="3998216at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="385" mass="43655" checksum="A874AD31BC8E6FF0" modified="2017-01-18" version="1">MNLQVSTGQAPLSPMEEYKQYEVTPGQMQKDRVPSNLNETSTSDARRENEGASIDKAVKENVQNIGTGKNNSTNSNYHNGQIDSRAPNAIITPVSGDTLSTTGTESSKGDSPQTISNKAGEAASGSDSWKVDINQIDQEVSKMLTQLEQIEQKFASSDFGIKRTNTEQQHQQQQQRSPVTTNAGLKEIHNLIDNFKSTILRLNLKNTLVKNQLLSVKDDYTMERCMLVNRIENLEKALRIIQDENNAVNSRNLKLIKYIRTLKNEKLKLYIAENRRLKEHIERLEQESSHNMNALRINHPQRYSSITSPMNSITSPVPPIPVPGGTSMQYPMAYREEDVDPPYHIRQQINSPTNFNMLDTLGRLATEYLHNEYPEEEQDDRLERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEW3</accession>
    <name>A0A1E3NEW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015221.1">
        <property type="nucleotide sequence ID" value="XM_019164924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEW3"/>
    <dbReference type="GeneID" id="30181611"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="125" mass="13989" checksum="9BC47F87198BE3A9" modified="2017-01-18" version="1">MVNPLAQIKLFADELGKDAGAGDPARLNVVEALLVKFLFAFVLVFCVYVYLDKTSVLNGNTPEENQRLHKMDYKKQLQEAAEKERAEDREKEERKKEERKKEEDKAGGKSSGVGAKSSGAKSRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEZ2</accession>
    <name>A0A1E3NEZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Complex III subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Core protein II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_74396</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015802.1">
        <property type="nucleotide sequence ID" value="XM_019164758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEZ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEZ2"/>
    <dbReference type="GeneID" id="30181445"/>
    <dbReference type="OrthoDB" id="6369905at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030061">
        <property type="term" value="C:mitochondrial crista"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF209">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="13">
        <location>
            <begin position="27"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="355" mass="37455" checksum="B238DDCD5D034E80" modified="2017-01-18" version="1">MFSVRSARHFSTSALRAVSIKTAPASSETVSSLKVVIKNAGSKSAPAGLSHLLASSTFFDTPEKSGLRLKREAELLGGEYSAEVTRDALVLKASFLKEGLPFFVNALGSSISKAAYKPHELAEVAGPYASHVYASKTTCPKFKALEELHAISYRSGLGLPLYYDGSKTYSSEDIKAFAKSSIFSENIEIVGENVEAADLEKFVSESPFATLPTGNDVNALPQPTYTGAESRIRQAGKTSAIIGLPVKDADAYELVAAGLLSSVPESITATADSKVLSYDGSSLFYFAVTSSDAAEVSSIIKAAAKDLKSVDFSKFSKLATLLAGKEVTSKSVSVPSDFNFVVVGDVDAVPLKSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF12</accession>
    <name>A0A1E3NF12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015271.1">
        <property type="nucleotide sequence ID" value="XM_019164863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF12"/>
    <dbReference type="GeneID" id="30181550"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="390" mass="44846" checksum="00215152419A0404" modified="2017-01-18" version="1">MFRGLKNIASDIRSEVSSTEQTVYAHTHSHSQSITGPGSARTKRFLPNSLSKHSSNGTDGGANGNGRGKSKYRLDEEFDGRTEDDTVVHRAFLRYFRDEIKSVELLISAGHSKIWLNCYKFGNSRSKGDDDFAKKLVMYLEEIWVGDSPEEHILFLTVSSFDFFKGLNNDQTQKCVVASYKKENADKIPLISYTKGLYGYWKMYPSGSVIRYESPQAKRVVQEITKNKKSNRSCITCTLNLDNANADPMRVETAMGNHIMGTLDKYLKFLSTAYFYLLNVDKYQKAVDLNKEIFEKIKLFNSYKLFIKYLLIYTNGKRVPREFLQAFEEERGYQPQENFNAVYATIPNKRNKYTDTMTRRLANGSVMVNGFIFTPQLISSLYKKLKDEYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFA3</accession>
    <name>A0A1E3NFA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015923.1">
        <property type="nucleotide sequence ID" value="XM_019164786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFA3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFA3"/>
    <dbReference type="GeneID" id="30181473"/>
    <dbReference type="OrthoDB" id="10012223at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="210" mass="23878" checksum="E330132BAE498131" modified="2017-01-18" version="1">MTGGSALVVLPSMFQSCNFVIGFINKKFILPNVLKRLFDHVIVSHMTLKYNKLPKGVLHCYLPGYYGETAVGRCIRKAVQISCPVTWFVRLIFCFFINMIPFVGPLLVILVRASRSGFSKHHRYFQLKGYTNAQVYFIWVHQKNSYFMFGFVALLLESIPGLGYFFVFANTIGAALWAVDLEDQMCRTIVSKMQEESDEDPSDLASSSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFD2</accession>
    <name>A0A1E3NFD2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015401.1">
        <property type="nucleotide sequence ID" value="XM_019160066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFD2"/>
    <dbReference type="GeneID" id="30176753"/>
    <dbReference type="OrthoDB" id="4038251at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035237">
        <property type="entry name" value="DUF5341"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17276">
        <property type="entry name" value="DUF5341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="304" mass="33030" checksum="3A0869D5208452DA" modified="2017-01-18" version="1">MGLNTSAKIIAFTVYAWLMKGALAAFSFRNIFRQDRAVVLIGASAVWGAVAAGVCATLGPTCWISLAMVAAFQAAGLAAMANDAGSVAENTKRSASMLPITETMQNITDAYENHNIMANPIIRNLTLLLGTVGEVSSGTQLVKRSDYDLAANITVAKFDSEFGHHLAIALPHLTSDEFVARLNSATPGEPDVSPRRRFFKRGYEVDWVSYNYDNVNEGLASQFINTQGASDTEVWSYGAESFISEHDGWKWCLDLNIDSSPEEPIDSNHLENINVAVHGEIYTNTYGGIDGQCNDYYDCVEGCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFM2</accession>
    <name>A0A1E3NFM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_179462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015256.1">
        <property type="nucleotide sequence ID" value="XM_019161821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFM2"/>
    <dbReference type="GeneID" id="30178508"/>
    <dbReference type="OrthoDB" id="3992052at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133337" evidence="2">
        <location>
            <begin position="28"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="336" mass="36307" checksum="D6479F06D61C2A01" modified="2017-01-18" version="1">MAAARNVCVSWTQGCLWAWVCLREGEGKTDVSAGWGDKRQAAIVEPEAWQRRGEAGLVLIRGCCCLFSVWPALSSPTHPPTPSHTPSAPTHTMFATHALRRTTAAARAAPSPARHASTTATPVDTTPSSTSSSSSTPAPRYVQLHHRRVSNTWNIPKKDLFLDMLFAAHGPLLEPVKAHVADRLFNSPLSRIYVTTHGHIRVSRPPTTVTDDLARFRALRSKSRHPNHHVFFSTTPLPAPASSSSSSSIFTHSAVSGLPLENNALLNLPHSLLLSLRPFCCPAQPGAESYRDDRIVVRKLQIEQNDAAVATVATAAAKRAKRAKGAKDAKGARKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFV7</accession>
    <name>A0A1E3NFV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="10">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Succinyl-CoA synthetase beta chain</fullName>
            <shortName evidence="10">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_17523</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="10"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016133.1">
        <property type="nucleotide sequence ID" value="XM_019161633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NFV7"/>
    <dbReference type="GeneID" id="30178320"/>
    <dbReference type="OrthoDB" id="1552at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000004">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="29"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="73"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="348"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="414" mass="45099" checksum="17DEA22E3087EDD4" modified="2017-01-18" version="1">MFSRAINKSFVKKSAQQVRNLSIHEYRSAALLKSYGIGAPRGEAARTPEEAYQAAKDLNTNEMVIKAQALTGGRGKGHFDNGLQSGVRLIETAEEAKDLASKMLNHKLITKQTGAAGKIVTAVYVVERKYALQEAYLSIVMDRKNQSTMVIASSEGGMDIEGVAKKNPDAIKKFPVEIDVGISDKLATEIASGLHFTADAVPKAAKTIQTLYKIFKEKDATQIEINPLSETKEHDVMCMDAKFNFDDNAAYRQKEVFSWRDTTQEDPDEVTASKYGLNFIKLNGNIGCLVNGAGLAMATMDVVKLYGGEPANFLDVGGAATPETIEKAFELILTEKKVSAIFVNIFGGIVRCDYVADGLIQATKNLGLKIPVVVRLQGTNFAIAKKMIEESGLKLYLEEDLDKAAEKAVELGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFY7</accession>
    <name>A0A1E3NFY7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cofilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Actin-depolymerizing factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17545</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. Binding to F-actin is regulated by tropomyosin. It is the major component of intranuclear and cytoplasmic actin rods. Required for accumulation of actin at the cell division site via depolymerizing actin at the cell ends. In association with myosin II has a role in the assembly of the contractile ring via severing actin filaments. Involved in the maintenance of the contractile ring once formed. In association with profilin and capping protein, has a role in the mitotic reorganization of the actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016157.1">
        <property type="nucleotide sequence ID" value="XM_019161638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFY7"/>
    <dbReference type="STRING" id="763406.A0A1E3NFY7"/>
    <dbReference type="GeneID" id="30178325"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000060">
        <property type="entry name" value="Cofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="11">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="143" mass="15971" checksum="0E1200A6B1ED916A" modified="2017-01-18" version="1">MSRSGVAVSDEALKAFNDLKLGKKYKYVIYKLNDDKTQIIVDNTSTDASYDAFLEEFPENDCKYAVYDFEYEISAGEGKRSKIVFFQWSPDTASIRAKMVYASSKDALRRALNGVSSDIQGTDFSEVSYEAVLEKVSRGAGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG18</accession>
    <name>A0A1E3NG18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_35617</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016200.1">
        <property type="nucleotide sequence ID" value="XM_019162953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG18"/>
    <dbReference type="STRING" id="763406.A0A1E3NG18"/>
    <dbReference type="GeneID" id="30179640"/>
    <dbReference type="OrthoDB" id="8093034at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF5">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="69"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="157"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="276"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="365" mass="40860" checksum="A04DA414E6F79678" modified="2017-01-18" version="1">MSADQIQQQDAAAQPQSPLQDQQQQQQERASTPDAQQDSTPSSSQIPLIAEETQNVASIADGGRELSKTVLYVGNIHRSVADETLQDLFSSLGNPVKSLKILQDKNRPGFNYAFVEYESPDFAEHALHSLDGTSIADNQLKITKAFQTQQVKNSDTFNLFIGDLSLEVNDEVLNSFFQKFPSLVQANVMWDMKSGRSRGYGFVCFDDKKDAEEALQTMNGYMLGDRPIRLNWASHRDRHMMNNQPNGNMNSMINRPPVMGPPSYEMVLRQAPSWLSAVYLGNLADYTTQNDLIPLLQNFGYIVNFKLLSHKGYAFVTYDTHERAAMAIVQLSGFTINGRPLKCGWGKSNKVVSDIRNISPDVRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGF3</accession>
    <name>A0A1E3NGF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_17707</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016330.1">
        <property type="nucleotide sequence ID" value="XM_019161704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGF3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGF3"/>
    <dbReference type="GeneID" id="30178391"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060258">
        <property type="term" value="P:negative regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12194">
        <property type="entry name" value="Kcc4p_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000394">
        <property type="entry name" value="Serine/threonine-protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.220">
        <property type="entry name" value="Fungal kinase associated-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031850">
        <property type="entry name" value="Fungal_KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043024">
        <property type="entry name" value="KA1_sf_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16797">
        <property type="entry name" value="Fungal_KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="40"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="404"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="467"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="617"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="864"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="927"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1047"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="720"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="408"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="467"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="648"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="864"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="927"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1047"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1073"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1224" mass="136044" checksum="919C633BF33362E8" modified="2017-01-18" version="1">MTPHSRQPSYASSFVSTSANSHQTSGIFPKKRTPVKIGPWKLGKTLGKGATGRVFLATNVENNQKAAVKIVSKAALTHDQSQDNEEQDCDSAGLSYGIEREIIIMKLLNHNNVLRLYDVWETEKALYLVLEYVEGGELFDLLVDSGPLPEKTAVEFFRQIILGAAYCHSLGICHRDLKPENILLDKDLNVKIADFGMAALESGRLLETSCGSPHYAAPEIVSGLHYHGAESDVWSCGVILFALLTGRLPFDDDNIRDLLLKVQVGEYEIVEDLSPEAVDLIHHMLTVNPEARIKTRDILKHPFVKRYYDCEKDDAFIQLPSPESAVSPVKSRQQIDKRILENLVILWHGRSSESIVEALLSPVANSEKTFYTLLLRYRHDHANSNRISKGNDLVRSSSVISKVTDPISASTSSSSSSNNSNGTKRKSLLITASTPNRRPISFQKNNRVSRSRDSLQTTEFSVANLTTPSAASHQRALSHQKENSPNNPNTQTPVKSFNDDKVHRRQSLIVTDVTPTKRRSRVPSEASSLKKMLATSNTKTRMKRNSVTSKVLSTYAVMAEYEKSKTSADNYGKRASSDFAALCDVLFGDESKANTKIGNSDSMATLNAIVFGDKRNSARLSTGSPTSKRHSRYESKSSKKSSAKRSSTGGNAPKNRNSSNPIQRISKLLNASELGDFERRTASDIHTGANNLTAPPRPVSRLDPRFKAFETYQKRTSQHAAQLLQLQEEAEAEAKALAEIKKREEQLKKEQEELERLKKTSVHRKGSELVNNTFNDELGLSSGTVLKKNDSANNFTYQQPNHTKLKSSRYSTLSMYSTKASSKRLSFYLKELDDEITKTHEQTKKDRISRLSLLVSDPEFAIQDRDSQFDTSHHNKSMNTLNEADEESSQAGLRTENIGAKPRSASISTALDNDLIFVDQHGDNSGTQNNISPAVLPLSSTAQGSNTKTGADATLGSSVYDSISPLPPKTEDSFATDSENTKQKGPKLPEIPGSPIKEKKNRTANKNDTFEIFEDAIRRSKQEFDESELKEALNVPLVASVNKPTEVFETKEPNSKPLKVEKQRQPLGARDANLNQQKGTTDNQRKNSFFSKLSHFQPDTERKSSFGKTFKSLFSGNQLSQPQSMVTVLTADDAFDAMKSLLVGWKHYGVGNLFIDSKHRMLRGELAKNNNLNIKHCKFLCVITPSKENESRMTFTHEKGSSKSFDKLIGEVRRILANENVLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGT7</accession>
    <name>A0A1E3NGT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016445.1">
        <property type="nucleotide sequence ID" value="XM_019161743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGT7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGT7"/>
    <dbReference type="GeneID" id="30178430"/>
    <dbReference type="OrthoDB" id="3987184at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="580" mass="64849" checksum="F4B71EF7CACEA579" modified="2017-01-18" version="1">MSSRQDRQQDTSESHLDRKLERLAKIYDLPLILNERLAIINSTTNLDTYQGLILPRHALSSNNSHLSGQQVSHIDFFDSDDLGSASVGGAIGANSSSNLNDGGIGISNHTHDDYDHDIGHSNSIDEFGAANSSHDNNGQGILSLADGSASTNASNSKLRNPTVLVGHGIGHNGGVPINNLSKIPSYQWGFKHEDLSSSNSSKYILNALGSRDSCSISSLAGNMPNINSSGLNSRMVRLPIKNDTSSNVNYYDSHNGLKVLGDTIQGGTERSLMNGLPYYNIEMAEQLIRKLNLNKKLGFKASNTNTSWFSDSMNETIDDIEYLHGEVILNQFVNNKRVIRNYQWLLYWNDKLGNKKLIDSLRNQNENVMSYTTYERLKNLRNKKFRKLQQKRSMIRQDMVKLDTAKEDKRNEYRVKFGAVPDNLDFLVNGEKSKIAKEIYGEELLRQEDELIEKENAIPEYEEELEISPSELEEAELEEKKEAAEGAAAASKMIPSSRFGGDSEFNKEQEIVLEKEFAVDDPKLLAIQNKLYGVHKSMRLQNEAKSKNGIITSKVVKIKRKPNPNALGFSNIRSCKPSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGU1</accession>
    <name>A0A1E3NGU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015676.1">
        <property type="nucleotide sequence ID" value="XM_019164730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGU1"/>
    <dbReference type="GeneID" id="30181417"/>
    <dbReference type="OrthoDB" id="3997858at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="476" mass="53992" checksum="B38C8CC01E1CBB50" modified="2017-01-18" version="1">MESLIYPSCAPVTSLSLVSVSTTSADRCQVNSRNLSHLDDIYKNSIFSHSFSATQSRQNQDGTETRQNSKSSIDSDISTASTTYSKSRSNSNLVTLSPSLKTAYLIKRGSFKKQRSFCNSIEDTIFDSSGFSYFSHRSSITSVSSVEGNGPHPLMVPESVIDDDESNKDRFFTKTSEESDNIDYASYTPQKEILKPPDEQTPSSLKKTTEGLLPFLKKLHGAIQRSSNSFYELKLAQLNLRSTEEVPLETFAQKFEALPTAHENVDLNPNLVDSENFLPLQLCGKTRRIREHRVNPYYLLQYALDTSVREKKLLNCIPNEELDIFDNLLLERYSELSESITFPLNEQIFDEMFYLKLQSQLQFFNNYENVNDEHIYHLKLASIARFKLCATITLPPRLDDVPSISSLIDDIYPTASLDTCHVPWLNIMDLKSGKAINRLTRSAGLLKGSNIQYVSKKCSSKRWVCVNNDQVDKQFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH92</accession>
    <name>A0A1E3NH92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nonsense-mediated mRNA decay factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17959</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in nonsense-mediated mRNA decay.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016602.1">
        <property type="nucleotide sequence ID" value="XM_019161825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH92"/>
    <dbReference type="STRING" id="763406.A0A1E3NH92"/>
    <dbReference type="GeneID" id="30178512"/>
    <dbReference type="OrthoDB" id="69928at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF0">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN EST1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="4">
        <location>
            <begin position="114"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="3">
        <location>
            <begin position="290"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="738"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="810"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="879"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="919"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="979"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1193"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="738"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="767"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="812"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="880"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="930"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1217" mass="136687" checksum="922386F3B0D9B127" modified="2017-01-18" version="1">MASDPTVMRRALAPAPEATQTMSVQDIDSIINTHKASIREILASTSTMADPSLLQGALSFCHSKFTAYIVDSLATEYREQVSAAGEEKDKDSASASDNVELNFNLESAHSVGNIVDRVWIEIHHPAIKYFQSTFYQLNKNLKDHGNNVANTNSRQYSGNKSSKYNLSGGSSQSFVEYRKLFDRFVKFIAKAYEFYFFILKSILNTYDLTIYIPVKKLCSTLKISLDSVSDAKDTRLPLPDSNHLVSSIVYLTHKCVLFIGDLSRYRTLIAKTYLPSTSISKEDNNNYSKSIELYKLSLLILPSLGDPYNHIAIIDNLKDDKFNVVYNFIRASLTSSPLTIAYSNLLNLLSKHPRTNSILRKFEHLNSLDRNTITKNDRLGLLKAQFLILFNYNLLPNKWRLKPGYLISGHSISLIENDFYYLLSTLDFHKQIFNDFYFKQFVILTGGFELLIDSSNLDDPKLGHSPEIISDYLSFMFRYMETFLKICIFICDDNKKDSKEAKDSSDNTSEKDDITQNFSMSFSTSLLPVIRLLLCWFNEREIARFYLQNSNEVSYLLATLINRLIKYFDEQKVDLVMELMSMRKEANITFDSILTLKPVRQRLFKEDVALREFKPVNYLLTDFNDAHLYRKDPESVLALIGELPEFSTAVETKDKKDKNSLVGVSTKINDNLLRLVAIIVLGKKLILENTNDIKWVDEINDADIDKTESKSHKFLGSLIIPKQVNLEIIEQVIPKVSNSNNGSSSAINSKSKDQNHKQTRKKKSDNPRSNNTPNNMSYAYAGSSFSTFGAAQPESFERFDKFKRNKSANKISVGRSQSSMASSSDSIVAGLATPGNTNDDLAKLKRPQTSPEFKIKFGDDSQSSHSQYVDMVASIVNEESDSRNKTFKSDRNNLKNKSREASSLNSNDLAVGNIGITKVNSVPPTEMSVNEYPENNNNGHTNGNYREGKLSNVNLPLERDYERGYTTYFNQWDSVKGKAASHEQNAENGTADQKAGEPIVNKGGSGNGDMDQMNLISQMSQINLMNQINMNQFSFNPMNQYGQIGGHFNPMEMNPVSLPNIHEGFNMNSMGVNNVNMNRLNNNINSIGHMNNMNSINGMNNMSLNHVSNMPPVSLDTMNNIGMNNIVGMGMSNIGNMGMNMNDIPSMSNLHTFGNLSNLNSLNGNLMGLNNNMAMPRIGGLANNLDMNNINSSSVFNDSTESDGSINNGNSKNKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH95</accession>
    <name>A0A1E3NH95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_140918</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016633.1">
        <property type="nucleotide sequence ID" value="XM_019160218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH95"/>
    <dbReference type="GeneID" id="30176905"/>
    <dbReference type="OrthoDB" id="3996044at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="406" mass="47239" checksum="D3E4A5F3E82162A9" modified="2017-01-18" version="1">MVSINNCSMNELKMSLMNNSSISNGPEKEKYFTFANPEINRSLHVVDADYGVFESNKRKRMGNLRMKTHNFIKKFKEKEPSNYVVLDDDEGDLRLRPEPELVRSHINQPRFNNIPARKSKHTPSYMLPTLDSDSDDYSLSSSSDSESVLPSSAKRSSDYDISQIKPSLQFSAQKRNLFQRQAELPTMQKSKVEAQYDALKLKAAKYRAERNMQRAKLENHKMALKLREYEYYCVNGIDHQQVNLDSGNDRMLEYGRDHLNIDYRQRRKESKKRAPFFRTTASSKHARVQPGFLDQLSRNLPPEAAMFIEYYGDMIPSTTTIREFHSAVLGWVYTVPLLSLVYPLLYILGTFIPRQTKRSWSFRALFVGFLDLCILLVTGWAIYNIFSYSAFFVSKLFQVVRFLRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHG5</accession>
    <name>A0A1E3NHG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="3 11">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_16867</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 11"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016680.1">
        <property type="nucleotide sequence ID" value="XM_019161371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHG5"/>
    <dbReference type="STRING" id="763406.A0A1E3NHG5"/>
    <dbReference type="GeneID" id="30178058"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170069">
        <property type="term" value="C:geranylgeranyltransferase-III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000012">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="12">
        <location>
            <begin position="5"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="321" mass="36022" checksum="AD45DBFCB18F2751" modified="2017-01-18" version="1">MSEKLLVNEHVAYVKDLFRHPAYLVHFMAEHLKMNALYWGLGTLFLVNHTDEFARDEVIHFVLSCIDESNGGFGAAPNHDAHMLSTLSALQVLKMYDAIGEMTSEQRQGVIRFVKSMQLPDGSFQGDQFGEVDTRFVYNALQCLSILEELDPSIVDPAVDWILQCQNFDGCFGMVPGSESHAAQGFTCLGALSIANALDKLPNKEILEWWLSDRQVDSGGLNGRPEKLPDVCYSWWVLSSLAMLGKLHYIDGKKLQKFILSCQDAEKGGISDRKDKEVDIYHTFFGIAGLSLLGYKNLRKVDPIYCLPVDVTSTIKKYPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHH4</accession>
    <name>A0A1E3NHH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16883</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016690.1">
        <property type="nucleotide sequence ID" value="XM_019161380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NHH4"/>
    <dbReference type="GeneID" id="30178067"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="142" mass="14861" checksum="209746CA9D829DE6" modified="2017-01-18" version="1">MATENVTLGPQSQGTSEVFGVAHIYASTNDTFVHVTDLSGKETIARATGGMKVKADRDESSPYAGMLAAQDVAVKCKEVGITAVHIKLRATGGTRSKTPGPGGQSALRALARSGLKIGRIEDVTPIPSDSTRKKGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHJ9</accession>
    <name>A0A1E3NHJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_16904</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016714.1">
        <property type="nucleotide sequence ID" value="XM_019161391.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NHJ9"/>
    <dbReference type="GeneID" id="30178078"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="397"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="903"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="966"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1014"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1302"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="903"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="943"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1021"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1355"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1400"/>
            <end position="1413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1413" mass="160567" checksum="B9A503CE77B1FE3D" modified="2017-01-18" version="1">MSQEHTSQLKDNSGKVKDRRIESVISDTDVRFNGKINSHLTSPSLKCANIDHIDDSTLKENVYSMTLNMHPQSNSHTPLLSSGTIPDNDIENITDMEDLDVENNEKEKEKDFDNASSNTEQNSEFDPAFKGEITETQEDENEQEMSTILQSSPFQILELRRELFKNTQVKKQTGLKYYYIDREWLTVFLNKEFKEDDLQIDNVIGPVKTIPFDQSLNEIVDSFIVLDQFKLLLEWFQLENNNQLIERELIYSTTEDKFIIDLHPLTIIPHIFCNNASQVNRFKQIQNRNHTFQISSFRTVADLFSCMLSYFDLTQIHVSSTRIWCVELDSTHSPLVLIPSALKYIKKRKLVSKKRRRSATLRQRHITSCHLMLEIKQPDGIFFLDIDSHVLLGSGLVGLNNLGNTCYMNSALQCLVHIPELNSYFLYHFFENELNKDNPLGNKGKVAISFGSLVTSLFDDRFNGNQSSFSPKEFKYTIGHFNSLFADYRQQDSQEFIAYLLDGLHEDLNRVLKKPYVEKPELEVGKEDNMEAITNLAEKCWEAHKMRNNSVIVDLFVALYKSTLICPVCNRVSITFDPYNDLTLPLPITKKWTHKIKILPENGIPKIFEAQLSRNETYSDLKRLISKYMDIQEENLIGVEIFRNSIYKNFEDSTSDSRYLPISELISSGEDVWFFEVEKIEGNAIIPVFSTVVSNSNRNNFCLPFFISLSPEDRFSYGTIQKKMVRKYKQLSTSELFVKIDEGEDGGYTRKDFEGIELFFKYLGVNTPSDEPKIEDEVIKSNADTKMIEANVSDDDDKSTISLASPDYTLENIFSSKVVNSSLEKNYYGRNTTARSFERSSSNSNKSSLDFWFPENNNTIIKHDLPSLLDGMSTAKKCYYLYGPENLRMLEEHYEKLDCEQVKNKDQDDESIDSKQKAFCPSDNVEMMDGSDTPNTESKSLEDVESECDIDEESKDPNFVQKSSFINTKDDINVPTKEQNDGSPIDQYFTATENIVSGKSDVGLHKSANVLQSGDHNDKLVSSSSSSISPVSTSSASSPSVPSSSSLSIAKDVCSVTNDSSEDRKPLIEPMSAIVNEFRENSFDLCFCNPSDSPFSGSETWSSPEKLVNDDLERERTENLENARKPVTLYDCLELFSKPETLGENDLWYCPQCKEHRQATKKIELWSAPDILTIHLKRFESTQSFSDKIDIVVDFPIEGLDLNKFVADQNGEHVYDLFAVDNHYGGLGGGHYTAYVKNFADGKWHYFNDSRVTPVNDPAESISGNAYLLFYRKRSRAPLGGDYFHNMMIEIQKRNGELKKAIDDSQVKQASSTSTLETSDEEMHTSETSESSENFEETQPGNGGTAERNIKNSNDDCDDESEIDADDGNKRRKLGVSSTIGEPVRNNRGNDVATIAEEGSASSTDTSFTPISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHQ0</accession>
    <name>A0A1E3NHQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_35736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016201.1">
        <property type="nucleotide sequence ID" value="XM_019162964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NHQ0"/>
    <dbReference type="GeneID" id="30179651"/>
    <dbReference type="OrthoDB" id="10264707at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052742">
        <property type="entry name" value="Mito_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138">
        <property type="entry name" value="ACETYLTRANSFERASE, GNAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138:SF2">
        <property type="entry name" value="PROTEIN SPT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="52"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="218" mass="25236" checksum="C3B276139F60C5C1" modified="2017-01-18" version="1" fragment="single">MSEFNNVVFSRNHYTPHYSTPYRIERVTIHLKDLDTTATIYPLFNINQAPTNLIRFLSQEYNDEILRGDTLPFFDKLAVEEFQNFWFGQFAAVMCIGDEPELSEDITVNEWAKRCLGSFFIKSAYPGRCSHICTTNFLVNAGIRRRGIGFYMCECFLRWAPTLGYSRCIIDLVFETNIGLKRLLEKCGFHKVGKVNSCAMLKSTKDILINSFIFTKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHT1</accession>
    <name>A0A1E3NHT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GPI inositol-deacylase</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_73203</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016802.1">
        <property type="nucleotide sequence ID" value="XM_019164413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHT1"/>
    <dbReference type="GeneID" id="30181100"/>
    <dbReference type="OrthoDB" id="10250441at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="3">
        <location>
            <begin position="612"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="957" mass="106107" checksum="97F5413B41101984" modified="2017-01-18" version="1">MGSGSGSGSARVSPPKPAAHLGSHGLDAADAPAKALKSSLNKSVSRASTGGRSSIRQISYSGEVDDDDDDVSIESYQPDDSKGYPEAPNDPTNKFSRQISREDASSIIGGINLASPNLSAKSSRVVSSIPSGRIQPTVVHRNSLQGTPKVSRTIPKIISQNEDGLESYAQDNDESHKVFNFSLPFSNPTNAAVAEDIENEDEAVADERQQENVGKKPSHARPKHHDDFSLITKTFRKLSHVLPFSAKSKSHSKARPVLQRRSSHPEAPDPPADVYVYWNDISHKVMPDEMLDREDVRLKLERQESLDTLQEARYYKDITHLDDGKFKALRQSLLPHAPSLSLSQSISSKISFVTTRIRSDSLTPTPTQSQALPIPDPAPKLAVTEAKDIALTPPNNVPSSAVVQNNNKSLTSLNNSINSTLIEKEPSATPSASTTTANNSHNPLLSTLATTTKFVTSVPMSPISSITHQTDASSSMQIYDDLDGDVIVLGGYRGSILRDSRTHRRTWIPVLKAGLNIRKINLMIGPSDDDELRELNARDVVFNRTPNISPDDKDYPDSYPDGMLTHIGPVDISRRLLKRLNSNPNVTARDWGYDWRLSPELLCEQLHSELISIVNKQKNKKGVILIAHSMGGIIAHGAMVKDPSLVRAIIYAGTPMPCANILGPFRFNDSILLSRDILTHEANFFMRSSYVFIPPSWGGEDENGEGGGMCLFRDLRSGKRYMIDFWDEKNWVLYNLSPLVSNVRLKQDIASGKLDLHSIKDTALKHEIKKFLKIKVNHDTESIVLDEISKLHEPVVLWLDCYMYLARTLGRTKTFIKSLERKDHVNYPPMAQIFSNGVPSVKYSLVDGEECIKRGEYYKFFYGPGDGVVYQGWNFPRERGRKRPNGPDRCGKGSVDPVANVPDWRGREYGYGEKYAYDLCGRFRSTAGHIGLLVDHDLVGQALNAVMEEEKKRILES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHV9</accession>
    <name>A0A1E3NHV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_166789</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016261.1">
        <property type="nucleotide sequence ID" value="XM_019161305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHV9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHV9"/>
    <dbReference type="GeneID" id="30177992"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="390"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="626"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="730"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="871"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="636"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="684"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="748"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="758"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="879"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="995" mass="111889" checksum="1F0584CE3D816318" modified="2017-01-18" version="1">MTVASSDYANYLHGGLLNDRYLRMKGLNEGSFGIVSIAKDTKEGNTLVAIKYNTGRLGDFEAYQNKTKSEDHIVKVRSMSLNSNTRSAIKSDATFSNTETSDKDVSKSIVLRETKQEIAMIKKVDTHPNITTLLDYFDTYMVFEYAPRGDLHDAIQLGIAPVATSDVIDVFMQLISAVEFCHKNGVYHRDIKPENILIADDWSIKLTDFGLATDHLICNDFDVGSERYMAPELLEHSDIDSYAADKVDIWSLGICLLNIVFGKSPFRSASSKDKMFLHYAANRETLFDIFPFMSYDLFSVMIHSLTIDPANRDLEMIKVNLLNIDVLTYDYEFEEDVDDIKAKALVEEIGEDESYEADGKQGNAKSEAANTAKVEDTISEIKPAAKGENPVVLFDNSPNNIDKGENATRTDPISTGANSEVRTNDGKSEIDMSEPKHSPNINPSLQPSAVLESANRKMGLIDTLLGTDTNNNYTVSEPINIQKKYEPPHKLLANSLNDSKKFNISNENRKANNNNNYRARRWNPAHRKPLKIANYNYRNKSQDGRIGGRDSLKDDSFDFNRKDFFTPRSVFTHYMEKANRGKQNVQQRGSYYRKNKEFENSSNNYTYNYRHAFNNTNNYYDYSDRRAWQKRKRRSSNVPSSNSNKNKNSYRSGNLRSRNSKWEAPLWNSSNDHKSKNPRRLSAGISSGANSHPVRVTSSLRSSMDGKYIPPNLRFNASNLLGSAVVSDDDTETDNDFMSGSSKRTSKGHFESPREIEDIAEDDDDTGDDQVEHAEDSDEETFSFEFDQPTVSRNNGMNFLAGVSPSDDGEKFEQIQSNAMNLLSKQFTESRLFDEKNNSRDTSLTTTVSNNFSSTGSNINTSPKGYKKYIPPHHRRSSHSAADTTGNLTSTLTDKPASNVIMNHFIDRSSNKNMSPVSTSAPTKSSSFFSSCKNIAPHELLTEKGYDAFVNSDQYNDNLFNIEEGNYITPTKMMVNGLSEQKFIANGSIDDKTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHZ1</accession>
    <name>A0A1E3NHZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_19530</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016854.1">
        <property type="nucleotide sequence ID" value="XM_019162126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHZ1"/>
    <dbReference type="GeneID" id="30178813"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="4">
        <location>
            <begin position="69"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="193" mass="21252" checksum="FC4D3D052E690AF6" modified="2017-01-18" version="1" fragment="single">NANPQVKDVSQLPTRQAKGGDSGKTEHYPHEVYRTRNLKGSCDDVDDASDEEEEEEDKDDGDSEPLDAQEVYDLISSISDPEHPLTLGQLSVVNLGDIRVRHPDYASGRVGEVNVQITPTITHCSLATLIGLGIRVRLDRSVDKAYRIGIYVKQGTHQSEGQVNKQLNDKERVCAACENPQLVKVVGRMLATC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHZ6</accession>
    <name>A0A1E3NHZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_25113</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016870.1">
        <property type="nucleotide sequence ID" value="XM_019162382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHZ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NHZ6"/>
    <dbReference type="GeneID" id="30179069"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="536"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="587"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="611"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="640"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="276"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="681" mass="78737" checksum="A9C8384C357B3EE9" modified="2017-01-18" version="1" fragment="single">TERIHLVIMTIAAIFVRLYQIGIQDIPSSRESQYALMINNYFTGEFFIDYNPPLAGLFYTALARFFGYDANSLDQFSIGVPYKLFPYCQLRIVSAFLGAISITFAYKTLRSTGVSHSISLFGSFLMMIENSMITEERRISDDGVFIFFISLFLSNQKIAEVKEKFTVGWFIHLFIASLSLGLVLSTHWTGVFIFTYAFVSVVIESWALVEDINVRFKYVWANLIVKSLSYFTISLTIYLAVFKAHFDLLSKKGPDYNKLSPRFQHSLENNHLEDIFSNVNFNAGVMFRHYKSGKYLHSHEDYYRSSNHQQVTLMDNFDELNNLFKVIRVDDESEAIPASSILAPWRVRLKHESTNSSLVIDKDHKPPLSEQEYNFQVTTDHNFGTSDDHTNRYSFLLKMAIDHCKGDDARYALRALDSVFQIYNEEAGCYLLGTPLVLHEGFAEGQDEVICIQEPSYEASLWYIDWNDHPEYAPNKKRVAFDPITFWQKLIEIHLYILNKLYLGNGYTDETVSSVGDLVLLKTGFVHWVDTVSHSVIYLLGNFIIYYMIVSSVGIYGLFKAYQLLTFNPFQASASLDSSNYKYDHKTLDYVILYFLLLIPLSFIKIDLYLFNYLPALFVGILIVAQLFQWGHEKAPKLTFFFMAFFSILSFLAYVKFSPLIYGLQWTHEKCLKMMVSPDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHZ7</accession>
    <name>A0A1E3NHZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016888.1">
        <property type="nucleotide sequence ID" value="XM_019161442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHZ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHZ7"/>
    <dbReference type="GeneID" id="30178129"/>
    <dbReference type="OrthoDB" id="66726at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="752" mass="85116" checksum="7E67F5BE2C8DCAE7" modified="2017-01-18" version="1">MAELIASTFSSQSSTGTSTRDMNSPTFTSIASLSITTPAATLAPKAGSESPWLILRLIDSFSLAVLQQSKESGINGNMNDGTSTFLSYWMSPFAMMCMITAVVMNRVVIFASSRRHRRLPYISNVLLRSFAIYILCLGSYGLFVSLKCYCHNPLIQYILQANCFTFDREKFMSKTFLSVPFGSNVYEGFLKHTNEMEIGPTTSVLKGFHLSLCISQILDTFIAVASGTKPSLETGITLFEYSLAFQEAQFASKPSVELLTIALIALTNQLNIHFLGLFNLIQYKLIPSSILGLFTLSFYAYNIFTGNILKIPFIIILGYFPHFCVLLVIMLSESIFLLSGIIRFSFKDLTMMSLLENWNSVNISLSDDFYTALINFGEFVINISISQCYIQETSNVKLPIDNYINMQLSELKKEETKEINGYGNEFKNNPELIDLYNSDKRNEMKKTNKRKRSWIFVTRLKMLNKVAFKFLELIKVKIISVIGKKAKDSDGTALDETVTRTKSIIKYRIVPTDKGGFVYASDEFESVDIDNLSVADIESSYPELLMNADLLESDNSPDYDYEELDDENYQESDNESDIDDSSETGHPGENSNLEFRSTTQSENNDTPEVLNELITLPDLKNLLFPSPAGNPITNQILSYHLQHLNEPVSHLTRSYFAKYYVDDIKLLDLLKEKHRHRARDDTSCVGERELDVHCEDLGICVICHENSRQIILWPCKCLAICENCRISLFVREFATCVCCRHPVEGYSKVYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI05</accession>
    <name>A0A1E3NI05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA hydrolase</fullName>
            <ecNumber evidence="4">3.1.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetyl-CoA deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17688</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Presumably involved in regulating the intracellular acetyl-CoA pool for fatty acid and cholesterol synthesis and fatty acid oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O = acetate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="3.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyl-CoA hydrolase/transferase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016311.1">
        <property type="nucleotide sequence ID" value="XM_019161696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI05"/>
    <dbReference type="STRING" id="763406.A0A1E3NI05"/>
    <dbReference type="GeneID" id="30178383"/>
    <dbReference type="OrthoDB" id="10250396at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003986">
        <property type="term" value="F:acetyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.70:FF:000002">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000003">
        <property type="entry name" value="Acetyl-coA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.20:FF:000001">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.70">
        <property type="entry name" value="4-hydroxybutyrate coenzyme like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003702">
        <property type="entry name" value="ActCoA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609:SF1">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02550">
        <property type="entry name" value="AcetylCoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase N-terminal" evidence="10">
        <location>
            <begin position="10"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="11">
        <location>
            <begin position="329"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="524" mass="58340" checksum="23243A7F2DC38CE1" modified="2017-01-18" version="1">MSAILKQRVRYAPYLKKLRTAEECVPLFKDGQYLGWSGFTGVGAPKVIPTALADHVEQNKLMGKLGFNLFVGASAGPEESRWAELNMIHRRAPHQVGKPISKAINDNRTQFFDKHLSMFPQDLTYGYYTRFKKDNNLLDYTIIEATAITEDGSIVPGPAVGGSPEMISVSDKIIIEVNTATPSFEGLHDIDMPINPPHRPPYPYMTVDQKSGLTAIPVDPSKVVAIVESTTPDKVPSPTAPDEMSKKIAGHLIEFFEQEVKAGRLPENLHPLQSGIGNVANAIIEGLEQSSFKNLTVWTEVMQDSFLPYFESGKIDYATSTSIRLSQEGFKKFFDNWDLFSQKVCLRSQVVSNSPEIIRRLGVIAMNTPVEFDIYGHANSTNVNGSRMLNGLGGSADFLRNAKLSIMHTPAARKTKTDPTGISCVVPFASHVDQTEHDLDVFVTDYGLADLRGLAPKERVPLIINNCAHPDYKDQLNEYYARALFHCTKTKSLHEPHSLKDAFKMHINLQENGTMKLDNWDVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI06</accession>
    <name>A0A1E3NI06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosylceramide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">UDP-glucose ceramide glucosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">UDP-glucose:N-acylsphingosine D-glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_59820</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016086.1">
        <property type="nucleotide sequence ID" value="XM_019163390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI06"/>
    <dbReference type="STRING" id="763406.A0A1E3NI06"/>
    <dbReference type="GeneID" id="30180077"/>
    <dbReference type="OrthoDB" id="1483400at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ44973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="469" mass="53540" checksum="8EE7D2C26E81EF2F" modified="2017-01-18" version="1">MTPIWSYVDSYSSSVDEVSTSSVNRLLEVLSIPLFILYIVVLVLVFIGFTQITFHFNKKAQLQLDFLVDDDSLEPVTILRPLKGIDSNMLSCLESSFQQKYPLEKLEIIFCVQSAVDPAIPIVNTLIEKYSNVDAKLMIDQDQKSLDFFGSNPKVNNLHKGYKAAKYDILWVLDSNIYTDPHTLRRSIYTLQNNKNNGSSVPAWLRRDKTVKMVTHVPIVASNSKHRINWGSKLDEMFMSTSHAKFYVALNRIQPAPCINGKSNIYKRSDLDKSVELITRQRILKGDGLKHFAKYIGEDHMIAIAIFENVDGCAGMSRDVVLQPVSGQNSVKEYIQRRARWLRIRKYMVSAATFLEPTTESLLSGIIGSFSVNVLLKDKSSINWTWFICHMIAWFVIDAIQYEKLINVTNNNVSSVIVPRKFHLGQFARFWALREFLALPIWVISMCGTDIQWRGQPFKILPDLSAEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI22</accession>
    <name>A0A1E3NI22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pantothenate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_34467</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016903.1">
        <property type="nucleotide sequence ID" value="XM_019162889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI22"/>
    <dbReference type="STRING" id="763406.A0A1E3NI22"/>
    <dbReference type="GeneID" id="30179576"/>
    <dbReference type="OrthoDB" id="498611at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd24123">
        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000115">
        <property type="entry name" value="Pantothenate kinase PanK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="48122" checksum="65637D68D25AB807" modified="2017-01-18" version="1">MQLDPKLASSSGAAGAVSESEASSLSDTDVPPLSGSNSSSRQNSIYRHNHCSNQKPLNVSNSHIVNEREQYEAQVVPETKDISLPNHAIDDIKELSIDIGGSLAKLVYLTKDPHNAGGVRLNFYKIETDKIDQFIRFIRSIMDTQFDHYKQTPTIIATGGGAHKYHDKLSSELNCRIQREDEMQCLIIGLDFFIRGIKNEVFYYDEANNKYIEIDQSDEPVGEVSPSVYPYLLVNIGSGVSMIKVTGPNEGNFERVGGSSLGGGTLWGLLSILTDCNSFDEMLEMASTGNNENVDLLVGDIYGMSYNKIGLKSNHIASSMGKVFKEVIDTEKGDLSREERLSRFKQEDIARSLLYAVSYNIGQIAYLQAQRFNLKKIYFAGSYIRNHYQTIRTLSFAINFWSQGEKKAYFLNHEGYLGAMGAFLMRRNDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI62</accession>
    <name>A0A1E3NI62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Repressor of RNA polymerase III transcription MAF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mediator of diverse signals that repress RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016924.1">
        <property type="nucleotide sequence ID" value="XM_019161456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI62"/>
    <dbReference type="STRING" id="763406.A0A1E3NI62"/>
    <dbReference type="GeneID" id="30178143"/>
    <dbReference type="OrthoDB" id="277029at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000994">
        <property type="term" value="F:RNA polymerase III core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.50">
        <property type="entry name" value="Repressor of RNA polymerase III transcription Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015257">
        <property type="entry name" value="Maf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038564">
        <property type="entry name" value="Maf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504:SF0">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09174">
        <property type="entry name" value="Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037240">
        <property type="entry name" value="RNA_polIII_Trep_MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="375" mass="42577" checksum="359DB9409509B744" modified="2017-01-18" version="1">MKYIDEFDIDLVNQALTFNTTDMHIQGTCDLFTTKPVGADRKLYKVLGKLYNSEEDTSVDTGLESRSNSNNIEERPTLKQTRQNSNVSVHSNNSNKSMDSLNSQFMQLKNRSFSYSYTKPVSALEEKRFKRAHSFNSPSPEPEMINSVMDSPFGPLNQSSSRKLFGYLIGALNATFPDHDFSTVQPNHFTLLNSTSDLVAKVNSFLISAGKSTGLDWVWQTINTHIDLDQCVCFQFDPQQSFTDDLGVIWCNMNFIFNKKKKRVAFLYFLATVLNETADSTTTLRRRNSKMGTLDEGGMQLGEDDSEYDLRYNNDYGPIYEDVFEEDDVNNVEDGANVLVQGKNGPEEFDFDDENDYAMSDDDMANENQDDEGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI71</accession>
    <name>A0A1E3NI71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016934.1">
        <property type="nucleotide sequence ID" value="XM_019164448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI71"/>
    <dbReference type="STRING" id="763406.A0A1E3NI71"/>
    <dbReference type="GeneID" id="30181135"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF30">
        <property type="entry name" value="PHOSPHORYLASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="324"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="658" mass="75347" checksum="355736F4289C49EF" modified="2017-01-18" version="1">MQKKDQEYSSNLNRSNYKFLSSTNLLYRENLPPEYRLPTLVSGYLPDLRGINTIDNEEQIPEVDFKNAVAKFEIQTSDCNQKGTDIESTRDCIRKTYSENELSNYDGEYGRRKSLSESKKLMLDWKPLSNQELSPRESLKKSCMREGIVDEYIPNFATFNLAKFGINENVIEDSDDELDVYDRKPLDVETNEILLSTESKAKPIEIETKNNFKRGSDSFMVSDESFKIAASPEREGDRLVLSSIPRNFSSLSFSYRRKMLVELLPDSLKNNSEYKSHITKIIRKNSTSASSFGSASSNILAPRRKAKRVEPDTNEIGSILLQSWRLGRVFDSGSFGIIRECININDMDDVKALKIVPLKHSLKCLQTFRSEIIMWSKIHHNCFVPLLDVKVTADYIFALMPLYDEGSLFDKVKFWETNRVQLADRFEDIISYLKDITDALKFLHENGIHHGDIKLENFLLEKNRARLCDFGMTNYDEAASRRLLENVDPKVEKELQESYLFLSNNNSSSDIASGVQSFDMMGSMSGVNDSTEFSSSTTILNPTDSDHKEHHFNIGSLPYASPELLQPCPFPVDCKADIWAFGILVYALLMLKLPFWHIYEPRLKLSIIEGNWQTTEWKSQLNENPQIQIANTLVTGCLRERNSRYSVAEISRLLSGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI86</accession>
    <name>A0A1E3NI86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_34220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016960.1">
        <property type="nucleotide sequence ID" value="XM_019162870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI86"/>
    <dbReference type="STRING" id="763406.A0A1E3NI86"/>
    <dbReference type="GeneID" id="30179557"/>
    <dbReference type="OrthoDB" id="268693at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14750" checksum="B5AD34F93B412682" modified="2017-01-18" version="1">MAPQLNPKIVKKHTKKFKRHHSDRYHRVGESWRLQKGIDSCVRRRFRGTISAPKIGYGSNKKTKFLRPDGKKAFVVSNLKDLEVLLLHTQTYAAEIAHNVSAKNRVEILARAKAIGVKVTNPKGRVTLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NID6</accession>
    <name>A0A1E3NID6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_17800</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 8">Nucleus</location>
            <location evidence="3 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016451.1">
        <property type="nucleotide sequence ID" value="XM_019161750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NID6"/>
    <dbReference type="STRING" id="763406.A0A1E3NID6"/>
    <dbReference type="GeneID" id="30178437"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01858">
        <property type="entry name" value="NGP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000559">
        <property type="entry name" value="Nuclear/nucleolar GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024929">
        <property type="entry name" value="GNL2_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="10">
        <location>
            <begin position="210"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="463"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="497"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="534" mass="60536" checksum="5A32C047E501A89C" modified="2017-01-18" version="1">MATGKTEKARRQRQGDLRDGNLRVKGENFYRDAKKVKHLNMYKSGGAVRNAKGEVIKAAYLQDTKTPTARVDPNRRWFGNTRVIAQDALSHFRDAMADKQHDTYSVLLKRNKLPMSLLDEKDATESPSAKILETETYASTFGPKAQRKKSNIAASSLEELADITSKDNEKFEEEQILKPTLGLMGGVDTDDYTAEAKEYIFSKGQSKRIWNELYKVIDSSDVIIHVLDARDPLGTRCTSVEQYVQKECPHKHLIYVLNKCDLVPTWVAAAWVKHLSKDYPTLAFHASITNSFGKGSLIQLLRQFSVLHKDRKQISVGFIGYPNTGKSSIINTLRRKKVCQVAPIPGETKVWQYITLMKRIFLIDCPGIVPPSTKDSQSDILFRGVVRVEHVSHPEQYIPDLLAKCQRKHLERTYEISGWKDSEDFIEILARKTGKLLRGAEPDETGVARQVIQDFNRGKIPWFVPPPEDTEVRTGDDKKAHHKRKRAEREAAALAAEQEEYNDEVEDLEKAEGSASEEAEDAKEEERPTKKAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIQ5</accession>
    <name>A0A1E3NIQ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_150499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017128.1">
        <property type="nucleotide sequence ID" value="XM_019160643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIQ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NIQ5"/>
    <dbReference type="GeneID" id="30177330"/>
    <dbReference type="OrthoDB" id="10674333at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="419" mass="46509" checksum="57834CD27A86B44B" modified="2017-01-18" version="1">MLLTKTGRLAGAAVWKGWAIGHRHTMHDEDLGRAPVQLIRGRGAPLPYQSNGKHTESVEAGAEKVEAGWERQPSELKTMRKTLSVIGDPLPLDYLRNGGDQVAERGEKPVEREVENEEEAVQRPAAQGRKPRALRLADLGQPLPLKYNPAFNESWRAEQRLLTQAQAPAPTQQHSTRRAKQTSAAGVYDFGTPLPLPYADDDAIETEQYLRWKSSAAPGAFDEWKTQQDGRSRDTSRIPSGAADEGDLSQPPRSANVTSSIEDAEAQPEERIRDAAIPEPMLGAEHEQRRQQQQEQEQAPEQAPEPEPEPAPAPEMAVDHEIQRYILHDDYRGLEEKLGDAEFRLRLLRYTRNLKRQYDALCRVLHAPAVDDDVDNGPYSDTAGPGFLSAPRRAVVLQSAALSTGAFVLLCTISYFLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIZ0</accession>
    <name>A0A1E3NIZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017149.1">
        <property type="nucleotide sequence ID" value="XM_019164503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIZ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NIZ0"/>
    <dbReference type="GeneID" id="30181190"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="44288" checksum="721531ED7BAAB725" modified="2017-01-18" version="1">MALWADKYRPKALAKLSYHDDITEQLSKLADSGANFPHLLVYGPSGGGKKTRIMAMLRRLYGTGSVDNLKVDVKTFVTSSNRKLEFNVISSPCHMEITPSDMGNNDRVVIQDLLKEVGQTEALDFSGLFHIAKKENESKENGGEENEKSSSPQRKFKVVIINESEKLSRDAQAALRRTMEKFSASIRLILICDTTSNIIDPIKSRTLGVRIGLPGKDAMRQVFESILSNEADAKRSFPEDWDERAIVFDHIIQECDGNLRLGIMMLEAMYMNHDSINVQTPVIKPDWQLVIESLAKGVLKDRSVARLTTCRTILYELLSHAIPGKLLLEKLMFCFIRLVDDYSGLHHLNKLKTDIVDAAALFDERLTLGSKDIFHLEGFITRVMVLLESDSTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJC0</accession>
    <name>A0A1E3NJC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MutL C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017323.1">
        <property type="nucleotide sequence ID" value="XM_019162097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJC0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJC0"/>
    <dbReference type="GeneID" id="30178784"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="1">
        <location>
            <begin position="15"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="208" mass="23935" checksum="C86E25D9664BE5B0" modified="2017-01-18" version="1" fragment="single">LGLSIHKNDFNNMKIVGQFNKGFIIVYKRDTDDILIIDQHASDEKFNFEKLIDETVFENQPLVVPQKIDLNTIERLTILNNLKIFEKNGFKFKTCVVGEEGEVESEVEAEHFKREELYLTALPYSKNTIFDLKDLNELIQLVEEKGASTMSIPRPSKVRSMFAMRACRSSIMIGSSLSRLKMETVVQNLSALDKPWNCPHGRPTMRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJD3</accession>
    <name>A0A1E3NJD3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017368.1">
        <property type="nucleotide sequence ID" value="XM_019161115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJD3"/>
    <dbReference type="GeneID" id="30177802"/>
    <dbReference type="OrthoDB" id="3994523at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="147" mass="16118" checksum="CD959BDBFEC6BA77" modified="2017-01-18" version="1">MANAEPPRPAGLSVEKKPSITEDLHLKSVPNSSEKDDNALKSSSGTQSKKSLTKIKEEDSATSIDNISVPAPTPLNKSDEQTRNGIVSKSESDDQPRKTFAEVTQELKAQLNKTAEEVEKSDNEFERLIGNLEKKIQALRVQLDSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJH2</accession>
    <name>A0A1E3NJH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72344</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017374.1">
        <property type="nucleotide sequence ID" value="XM_019164166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJH2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJH2"/>
    <dbReference type="GeneID" id="30180853"/>
    <dbReference type="OrthoDB" id="272977at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048630">
        <property type="entry name" value="Sec8_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20652">
        <property type="entry name" value="Sec8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec8 middle helical bundle" evidence="7">
        <location>
            <begin position="266"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1013" mass="115725" checksum="896970363479D562" modified="2017-01-18" version="1">MTEKASASLQQLQGTLTLFESDWPDLLNTDCNPLDIALPLIDENGLLNYKDFNDLRRQFTQQLQRAVNANYMAFNDSIGSYGISIETLNQSQDRLSNIRECLDSVNTMLNSQANILGELNTKRMEHGQILNMLEEINDVQKKLTNLQESVENREFEKSVNLVKEIQEIFSDNRLSSIDGLQSLQLRLNSSVSLLLDGLIDEIGNYIYLKNSSNINAENAATSNRISVNKGLLAFIKKLNGKSIGEDEELEIQNGRYDELLARFKFVQKLNKESECLSSLIDNCDREIKQIIKRSVNEVKNKFPAQIELNSSSLSDKKNPFASFNLLQGLNGVIIKEVFESIFSRVLFLIQRHIVIYEIAKISGYKYKISKILREVQKQISLIIFNYVIDENLLNDLEELDLKSKNKTGNTPFDKVPKQFESIENGPMFQFSKLSITSLSGDLIDSLNNIFVSQGQGIISSEFTFQNKMDSSIFIGVDDVNESKKNILVPPNIFNMAYIIDEFISFTNNLLTIYPSTEQQKTNMNGVVVFFNQFMDVVFVSQLENSLIYQFDKLCEHKWKTDQLLDASLSFEQFFNKVTILLDTTLYHRPSYVHIIFKLLYKISDKFKDIQETLLKTNESKLLSKWINDSKLKMISNDIVKNLLSNSQTSEEFQNLDSLQSKELTYALSIGGTYIPAMNPNNFLTLERMKSLVDLLASLSNVLAWLPKMKRKAPELFQGIDLVQKLKETWSLSIFNDSEFPLTVVNLDNENNKSFKINSSDDNNKDSNGTYLNNEIEADIQGFIALDTQSGKEFEGIVNTLKGLMNDVEILIRYEIRVECIWCMIQMMVNKQWEQQGDESVDLGVDKFCDKVNNISRIFTRISKNIMDKSKDEIRIRIFGGLGFWIDKLSIFESRRIEVMGKSGWMKMIVNLRVLQQVIKSIDSDIQSDFEIGGGVMNGSLKYFTLGMGGERFIMSIEKLQSEGLSFSEEDWKNLIRLVYSEKLKKEGGGNVSKKYMAAQARLLKTLAVTIAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJR3</accession>
    <name>A0A1E3NJR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016921.1">
        <property type="nucleotide sequence ID" value="XM_019161455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJR3"/>
    <dbReference type="GeneID" id="30178142"/>
    <dbReference type="OrthoDB" id="3366093at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039295">
        <property type="entry name" value="MSB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778:SF1">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133392" description="SEA domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="805"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="484"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="571"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="625"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="665"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="716"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="767"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="807"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1164" mass="119772" checksum="7EBA71E4E33894F8" modified="2017-01-18" version="1">MLSLTKITIPILLVLANGAYTAPVDSQTTSSSSMSTSTSSTGTSTSSSDPVNDFYSSLTAMFNQDTSSTAAQTGTYDTTSSTPTTTTSSEQTQEATSTTSDDTTTSSPSPTTTSSSSTENFWAQDTSSSSTQNTWPQDTSSSTQNTWQQDTSSSSTQNAWEQPTVSSSTTQNYWEQPSPSPSSSSSTESPFKELFGYSPTDSASSSIPPSTESATSSSSEVTPTDYHWSPSSESPSPTPTISSDTTTNYGTDYSSSETDSFTLSASSVDPTFSQSTNEAYSPSEATATSSSSSAESSINYAPKKIDVTGSTTADSTTSSSYPESSASSASSDSALSSNLSDSVTESSSQALPSSSSTQSPPSSSSSSSSTSTSALGSAGAMNYGPSYYSSQSAESPATSSTLSTALGSAQPRYGPAPFSSGAKSQIWLPSSTSSTDAETSTFSSSSYETPSSTYGPELSVSSTYSFGPALPSSTFSEYSYGPTASTASWDESSVSSSSTFVPAPFSAAPVGPQLPSSSTWEPQSEASSTDYASSYYPISSTPAEPVWSSSVPIGPVYSATSSLDTSYGPTYIPSSSVEPPVYSSSSSSESIAYSSHVPEAAPWSTSSVVPTEPTPLSSAPVAATSSYVPSNVSPVPTESSTSSFAPVPSPSSDLPIASLTSSELPPISSAPVSVIPSSQDVAPSAPSLSPSSTVESATLSSATAVLSSVVPPPSAPSSTSVNVEPSTTKTGAKSSSATLPAPSSVVSETMTAQSSPTAPLKQSSTVSASAPPALEASSSAQASTSPLFSQAPLTSSSAGLPSPAIITSSAAPLPTDPATAPTDLSSSPTRTRVTESWLPTHIIAEDSTTTNTNTASQTQAVTSSLPKAIIPTQSVSQSSGFELITIGFKQALNYAFIVENSLSSAQIFEYLPQVLCFPFPEIDESAVIVKKLVPYSSTQVGYIITVAEVYFNQSYVDLLQNEILDLTSKIYNNSDATEKSMALLIDSKIPVTNLLESLDNPQQQNSNSNNGNEPNGGGDPAVDSLGSIDSAAITASTIPSDRSRNHKYIAGVVVGCVAGVGIYLGVIIFFLKRRIRNAAEQDVPDTSFSSMSDESLDSAGAPYTREKLEYSSSVGDIGLPDAENSNTQSSSFLQMFGFTKPKEDQPRFVPEISAPINVKSSLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKD7</accession>
    <name>A0A1E3NKD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M16 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017720.1">
        <property type="nucleotide sequence ID" value="XM_019161215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKD7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKD7"/>
    <dbReference type="GeneID" id="30177902"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF126">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="1">
        <location>
            <begin position="30"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="2">
        <location>
            <begin position="180"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="47820" checksum="27CE5F46E1CAC283" modified="2017-01-18" version="1">MLSKRLFSTSSRQLTKAAVSRLGNGITVITKPTAGAKASVGLYLNSGSRAENPYNSGISSLLGNVVSSSKAAADALKNEGVKYYSSNQKEVTGLLAASYPKGNAAGALASIKNTISQLNAIVSEDVRVADQKEKSIAVTEAFENSPKDMVIEHMIGTAFQGTSLALPKYGKAESLEILESEDLKNYLASNFVSSNLAVVAVGAEIDHDALIKFASELPVPTGAKTAFEPASFLGSDIRLRDDNLPQAHAAISVLTPGSKDSAAHYTGLVAAQINGSYEGANSPYSHFVGSTLAQFLEENHLIDSFDHFQFAYSDVGLWGGYFASHNVGNYDETIHFALKNWNRFSTGTVNDIEIAKAKAELKLRLLTPPECPVQLAEKLATEAFLLGYVPSEQEIAANVDAVTSSSITGWAQKYLFDQDIAIAGNGQIEALFDYNRIRNDMSLLRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKQ4</accession>
    <name>A0A1E3NKQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_27547</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017261.1">
        <property type="nucleotide sequence ID" value="XM_019162500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKQ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKQ4"/>
    <dbReference type="GeneID" id="30179187"/>
    <dbReference type="OrthoDB" id="4504960at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028927">
        <property type="entry name" value="Man-6-P_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF0">
        <property type="entry name" value="MANNOSE 6-PHOSPHATE RECEPTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02157">
        <property type="entry name" value="Man-6-P_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="10">
        <location>
            <begin position="9"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="202" mass="22663" checksum="CEFF9AA10EA5556A" modified="2017-01-18" version="1" fragment="single">EEAEKEVVEPCTVVNPLTNQFYDLRPLSALGNDGEVQTWNSKGFDYGKNFSIGVCSTPLRQLKALRESDFVDTHNKSMVGGYYTDGEGKKVSIGEYSTDLKFRGLNMVLEYTNGDQCEDSPNLSKSTLLTFKCDREMMSKARVSYLGSMNNCSYLFEVRTVHACATANDKNDSAIWGIFLLVLFSAAGVYFFAGFMYRMIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKR5</accession>
    <name>A0A1E3NKR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component CSL4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_72782</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017855.1">
        <property type="nucleotide sequence ID" value="XM_019164294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKR5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKR5"/>
    <dbReference type="GeneID" id="30180981"/>
    <dbReference type="OrthoDB" id="440760at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019495">
        <property type="entry name" value="EXOSC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10447">
        <property type="entry name" value="EXOSC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0271">Exosome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exosome complex component CSL4 C-terminal" evidence="4">
        <location>
            <begin position="114"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="259" mass="28649" checksum="91851979AB81A23D" modified="2017-01-18" version="1">MTALQYPEKVLPGQPILPIYQPTENSESIKFLPGENVRLKSITFDNQNIPTLVSTTLGYVVITDEIDKSSADSESPAVAAAHNVKVVSVIARKEKQSQNHVNDIKCRSYKAVTPQVGDVVLARVSKITHQRVNVEILSVLPKTGEGEDTRQRSEDLQLLNLLPTESGEYFKALIRSQDVRSTERDSVKTWECFQPGDIIRATVLSLGDGASFYLTTASNDLGVVFARNEDGELLYPLDWETMVAPGTGEIEKRKCAKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKR6</accession>
    <name>A0A1E3NKR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_154171</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017271.1">
        <property type="nucleotide sequence ID" value="XM_019160818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKR6"/>
    <dbReference type="STRING" id="763406.A0A1E3NKR6"/>
    <dbReference type="GeneID" id="30177505"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15535">
        <property type="entry name" value="PHD1_Rco1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15534">
        <property type="entry name" value="PHD2_PHF12_Rco1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052819">
        <property type="entry name" value="Chromatin_regulatory_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47636">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47636:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="180"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="549" mass="62766" checksum="F27488B11E415063" modified="2017-01-18" version="1">MLTGRRSRSKSKSDSGKEEGSSDAETRGGFVEPVLAECNIVTGQKMEDPPSVKVKKELGAFKRGTKHVAETPAVAERMVQYRNSLKSSKEIEKSTNLSEIRTSLKREHDENTDEPYDTRKIRKNSAKTEKKLGRKGDVNTTKTKSFLSAPKDVRMGKEHGIDAESEATPPVQPDSPALNNDYCSCCGMTGMFLCCESCPKSYHFQCINPPVDSNNLPDSWFCRECTKKKVLEEEGRNGGSKSIMLNVGVYSKLFDNIVFQDPISFQLPKEIIESFQGISIDRLCDYNDESFKPTKTYKQLVKEFDDPLHGIYDKNNNPLFCYYCGESGVKKELINCDYCPLTWHLDCLDPPITSVKKLGSKWKCPNHVDDLEKPIRRFKDQQIVQVSNIKSFENSGKLPVNSNIEIINIEDKLQALKDQMKLLHDNNNSSLKFSNLTFQINEEDIILNFINANKIRKLNENDENFQTLMNLQPNLKDYVLSLSQLSKKSLLDNEFRLLNLQHLLKVSDEELKVEKKDFTDEEIKEFQIIKRLIQQKGKEKLMKFLNFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKU7</accession>
    <name>A0A1E3NKU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72789</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017862.1">
        <property type="nucleotide sequence ID" value="XM_019164297.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKU7"/>
    <dbReference type="GeneID" id="30180984"/>
    <dbReference type="OrthoDB" id="2113814at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000005">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000082">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000003">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015348">
        <property type="entry name" value="Clathrin_H-chain_linker_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF1">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09268">
        <property type="entry name" value="Clathrin-link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clathrin heavy chain linker core motif" evidence="8">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="536"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="685"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="832"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="978"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1127"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1273"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1422"/>
            <end position="1565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1663" mass="187320" checksum="48D137081101816A" modified="2017-01-18" version="1">MSEAPPIELVDVCQLSSLGINPQSFNFLSTTLESDHYVCCRETGESGNAAVIVDLKNNNNVIRKSMSADSVIMHPGQMVISLRANDTTIQIFNLENKQRLKSNIMSEKIVFWKWLDDQTLGLVTASSVYTWNIFDGSDAGPVLLTSRHVSLNDCQITQFSANKNLNWFAIGGIAQENNRIVGHIQLYSKDRNISQPIEGHVCNFTQLKLQGSAVDTELFAFASRTESGGILHINEIDHKDGAPAFAKKSVDVFFPAEVGNDFPVAIQLSSKYGVVYILTKLGFIHLYDVETGKKIFLNRISSDPVFTGASFDNDGGIITISRAGSVLAVDIATDRIVPYILKNLGDVELALSLASRGGLPGAENLFSQQFNQSIATGDYQSAVKIAASSPQLRTPDTISKLKSLPSQPGQPAPFLQYLVFLLDRGSLNKFETLELVRPLIQQNRVETLEKYLKEDKLDTSEELGDLIKPLNITLALAVYYKANAPSKVIQCLAELGQFDKIIPFCEKVSFTPNFTVLIQNILRVNPDKAAEFATSLLQSQPDINIQQISDIFFSQNYIQQATAFLLDALKNDKPADGQLQTKLLEVNLLHAPQVADAILGNNMFSHYDKPTIATLCEKAGLYQRALELYDNIKDIKRVIVHANALPTDWLVSYFGNLKVDQCVACLREMLSNNIAQNLQVAIQVATKYSELIGPITLIKVFEDYNSVEGLYYYLSSIVNVTQDPEVVLKYIQCAAKLGQVKEIERVVRDNNIYDGEKVKNFLKEAQLEDQLPLIVVCDRFNFVHDLILYLYKNKFFKFIEVYVQQVNPSKTPQVIAALLDVDCDENIIRSLLQSVAGQVPIGELCDEVEKRNRLKILLPFLESTLASGSQDKAVFDTLAKIYIDSNNNPEKFLKENDQYDTLVVGKYCEKRDPFLAYIAYDKGQNDDELINITNENSMFKYQARYLLKRSDLQLWAKVLTSDNIYRKQLVDQVIGTAIPELEDPQPISIAVKAFMDNQLQSELIDLLEKIVLEPSPFNDNPSLQALLILTAVKVDPSRINNYIEKIDAYNPDEVAPICVQNQLYEEAFEIYNKFENYSAAMKVLVEDIMSLDRGEDYAEKLDKPELWSQLGEAQLNGLRIPEAINSYLKAEDPSNFANVVEVSEHAGKEEELVPFLLMARKTLREPLIDGAIINAYATLGKLGEIERFLKLSNVADLDNIGDKLYDNGNYEAAKLIYESISNYSKLASTLVFLKDYTAAVDCARKASNVQVWKQVNQACIENKEFKLAQMCGLHLIVHAEELEPLVTQYEYNGYFKELISLFENGLSLERAHMGMFTELAILYTKYEPEKTMEHLKLFWSRINIPKVIRACEAAHLWPELIFLYCHYDEWDNAALAMIERSETAFEHSSFKEIIVKVSNLEIYYRAINFYTNLHPTLLIDLLTVLTPRLDLPRVVKIFQKSDNLPLVKPFLISVLEKNNSIVNSAYHDLLIEEEDYKSLRLAIESYDKFDSIGLAERLEKHDLIFFRQLAAVIYRNNKKYNKAISLLKADGLWGDAIETAAISKSPKVVSELLTYFVETGNRSCMVALLYSCYSYLEYDVVLELSWLHGLDDLTKPYQISIAKENQQKIDAVYKDLKVREEKENAKEETSTLGQPLMITNGPVGTAAPLGLQSTGMGFGNGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL23</accession>
    <name>A0A1E3NL23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16654</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017950.1">
        <property type="nucleotide sequence ID" value="XM_019161293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL23"/>
    <dbReference type="STRING" id="763406.A0A1E3NL23"/>
    <dbReference type="GeneID" id="30177980"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="156"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="25550" checksum="340138400A013458" modified="2017-01-18" version="1">MSAEHTVYIRNLNEKLTVSSLRKNLTALFNDAGFRVLDVQACKNLKLRGQAFVSFAGTVDVNSVVETFNTKMVCGKPMNVRVAKAESDLAAEKTLTGEKYKQYIQRHREARLLRRQQEKEKAGSKKRGLDRDDDSLAGGKPASKRTKTQEKAVPNHILIITGLPKDTQEQDLIDLFTRYSGFLTVNLVLVRQLALVEFQTEENAVECVDELGTSVSVKGQTCTLAFAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL51</accession>
    <name>A0A1E3NL51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_72905</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017988.1">
        <property type="nucleotide sequence ID" value="XM_019164325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL51"/>
    <dbReference type="STRING" id="763406.A0A1E3NL51"/>
    <dbReference type="GeneID" id="30181012"/>
    <dbReference type="OrthoDB" id="3364892at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF26">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G07710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="156"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45834" checksum="2F3B237546992B92" modified="2017-01-18" version="1">MFNDKDERQQDQQQQKAGLSHRSTQVVSASSASIRAILLQLTSIYIRTPAKLFRPARFDYLTVSRMLLANELAGKPYHIFTHSSPALLYESVRRRGWQFIPNQVLPPIIANCAIGVVLYSTYLTFLQYFTSNRGWKWTLSGVTTGEGSQNWYSWLLSFYDSFRAGFIAGAISSIAASPIDALYSRSNYAELVDGDVKNNGIYKYAWKKLKDIGIVGIFAGFWLNLIKESLGFGFYFSVFEVIKSQGYSRTKSVIDWFDRIRGLDVDPNDVNTRKSARALQLTFVLFAGASAASALIAVQYPFNKVQKIHLARLEALDIFNEANKIETSKWFRLYYNSYKQTLDILIARKLKAGMSWPAFMYRGFTRFTLTNIPATSIGLLVFEITRQKLSIDLQDSLKEKLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLG2</accession>
    <name>A0A1E3NLG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_139707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018074.1">
        <property type="nucleotide sequence ID" value="XM_019160157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLG2"/>
    <dbReference type="GeneID" id="30176844"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133412" description="Hydrophobin" evidence="2">
        <location>
            <begin position="19"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="15863" checksum="BCFCBC81915F8399" modified="2017-01-18" version="1">MLVFACVSLLCAARQVAALCEKESSARQTDGQAVTSSEPLTAGNGGVETDVGTPAAEVDSTTGCNCSSSSSCPRSPAARSAQQLISTDRTPGVAAIAAATLQNTTAQLQRLPVVPCCSNCNVLRRQLLSIRSALVVHVHCFSPAVLTAHPLLSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLH4</accession>
    <name>A0A1E3NLH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018108.1">
        <property type="nucleotide sequence ID" value="XM_019159861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLH4"/>
    <dbReference type="GeneID" id="30176548"/>
    <dbReference type="OrthoDB" id="10252687at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="229"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="422" mass="48365" checksum="5376BA338DBD3837" modified="2017-01-18" version="1">MTWQQLLDELCTAISSHDTLKLAMFLLRIINLQDMEDFRPEQTSLDFMLINRSIAAAQRGGGGSGDEFFQQLFLLCMDLLVALKSGNVDTQIRCQTEQINLLSKRLLDEPSFFLMPIIIVAKNLRRVVFSNYPPNSARMLDDSTATPTVDSFTNAIQRPFKLLISNKSDNTINISAIHIFASHLFSIYFKYNKLNAVINLYKVLSNTVLSDTARDTRRIEMSPMLPTQSLFDYFIGLSLLVQSNYEKSFAFFNSAYQNKHSRSLDLKILFYLLPLNYLISKKLPTDAFFQKHPDLKVIQPLYTCIKKLSLSDYNKNLEAFKFLLLRFKVYSLYIKFQTMIQLQTLKTAYAIYKDTQTPEQLQKAHIVAFSFFTTSLNLGTSCTQTDFDTECLLCRLIATNNIKGYLSHAKQVIVLSKSSPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLQ6</accession>
    <name>A0A1E3NLQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">EB1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_21440</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018195.1">
        <property type="nucleotide sequence ID" value="XM_019162203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLQ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NLQ6"/>
    <dbReference type="GeneID" id="30178890"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035372">
        <property type="term" value="P:protein localization to microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="13">
        <location>
            <begin position="176"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="170"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="28757" checksum="286C859DEEFFA12F" modified="2017-01-18" version="1" fragment="single">GVSKSALLEWINAYFDINYTKIEQCGNGAVYCMIFNALYPNIINVSRIHRSPSSEFETLTNYKLLQHGFNKVRISRDVNVEKLMRCRLQDNLEFLQWLGKLWCEHNKDFTFSGSTSRPASRRTSSIGVDRNPTSKPLGSRKSSNATPQQRNDFRVNAATDNNGSNHISEDKRLLTEVSQLKQKLQTANEALEEVSNLKECLQIERNFYFGKLREIEVICQNISQTDNAEQISTLALIEDLKDIMYSTEEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLS1</accession>
    <name>A0A1E3NLS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017631.1">
        <property type="nucleotide sequence ID" value="XM_019164241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLS1"/>
    <dbReference type="GeneID" id="30180928"/>
    <dbReference type="OrthoDB" id="10649857at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="37267" checksum="B35F6CCE79E1F9CE" modified="2017-01-18" version="1">MVFQPFLIQNATAKDVCGYVNILFGSTEEQNRFALKIIVTDIHKIAKEFIASTADQLAEKTFSINYLSIDDKRMISVILAKMREIKQTFRPVFSSKVEEGEVENVHFNSQTNILTFSYNEFQYKFPLTDMSTNDQLDLTRQMYSNLEMFSLLQSNAMNSLSNQIINKNQIIKKLAYTYCQKMDPFGTRPESVHDLLKNKHISQIFVSRKLTRYLETTVSDAIQQSFKYRQTAANASLELVNPKWEMIWSEAVNEQGKHSGSDHTKESETEKVQIKDFGSETDIEETEDIAPSSSTQPTTKRKLQGLLRRNKKRMKTTSSNESVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLV4</accession>
    <name>A0A1E3NLV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3 14">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_32908</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase ALG3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3 14"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017436.1">
        <property type="nucleotide sequence ID" value="XM_019162792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLV4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLV4"/>
    <dbReference type="GeneID" id="30179479"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="455" mass="51993" checksum="118CB7A96951C057" modified="2017-01-18" version="1">MSAVSQRRVHVTAITENRPGILQKAYRTIKHLIWTVLYDPDIVGPVCVLLFLLEAMLLEFIIIKVPYTEIDYSTYMQQIGQIERGQFDYSKIKGDTGPIVYPAGYVFIYSWMKVVTDGMNNIGKGQELFRLLYLVSLALTFLIYIQASGIKKIRPYQLYLLVLSKRLHSIYVLRLFNDCFATFFVLGTIFLLQVAAKLKSKSYCFGVSVKMNALLYLPGLVIVLYFLNDENLVKLSGMILFGVVVEIGINFQFLTQSATVRSNFIHNAFDFGRQFMYKWTVNWRFVDKETFQSKSFHNLLLALHVSVLLLFVFARWLHRRETGKAPTELVLRDGLLRFYKNTMDPKHAILHPAGGARYVALVMMTSNLVGVLFARSLHYQFLSWYYYSVPFLLGEAGFPLALSVLLVAVHEWTWNAYPSTAASSISLVCVLSVLTFRLLFAISPPPPTSDKAKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLY8</accession>
    <name>A0A1E3NLY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71262</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018267.1">
        <property type="nucleotide sequence ID" value="XM_019163877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLY8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLY8"/>
    <dbReference type="GeneID" id="30180564"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="683" mass="76771" checksum="80466F2F4B0FD6DC" modified="2017-01-18" version="1">MGTALFGKFKLATLTQKKAGPKKVERKTKSHKPAKTENEKLLERKTRKRSGCFCCRKRRIKCTGSKPSCDNCVKASYLCVWPNGDESLPHNTDFKLIKVNCGSQEEVKNEEQSIEMASFESSELCSPDDPLPLVRHSSPEAFGALMTVAAKHKSSEVVEDALFRKFLLEINSSVLLQVYPQLTTFPYLSPQDTHLYDAFVNGFITDISPQLAHFKLQPASAFIPSVIDNSIVQGLFFASGAAFLYSSTKNVEMRKLSTQKFIESAGQLNSYISTHDITGNSDWIIIYLLVAYLKLRFIHEGQRTETLSMISIIEAMKIWLTNKQKRSAGDERGSVPKITELDIEPVFDEVDGESMKIPDLGPPEGLDVESDTATAFSRLAKRFKLISSHCGPSSTVVANQPSVPVDPSSVETEMYLAFNEGNNKDKSSRVLLPYERTMIESFAFNYSSLMFVCDKSLIPQMTSPFVIFDMLRPYLSVPIYKCAVPWMNHPVAGAALPIFELQAKVCWLGHFSPLSEEHKKLVSKIRSVANFNTRPILPIEVHTKEPENVQKKLLESCYAAEFVSKAVFIYSTKLLYPDMEYDGELIQSTVEQAYENLLNISIQSQIHMILGFAFAVIGSVAVQKKHREYLVWKLKRLATVFCVSTFDTIICFYQIAWSKTADGTRERGWDTLDDAEAIKTLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM32</accession>
    <name>A0A1E3NM32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_32115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018315.1">
        <property type="nucleotide sequence ID" value="XM_019162742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM32"/>
    <dbReference type="STRING" id="763406.A0A1E3NM32"/>
    <dbReference type="GeneID" id="30179429"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF5">
        <property type="entry name" value="MEIOSIS-SPECIFIC APC_C ACTIVATOR PROTEIN AMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="4">
        <location>
            <begin position="2"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="342" mass="37907" checksum="FFF6E753AA1B4767" modified="2017-01-18" version="1" fragment="single">VLDAPGLRNDYYSNLVCWANKSDTIAAGLGSIVYCWSETLGTIPLQTFGNDLISALSYSSDDFLVVGTKESKIYLYKPKSIHVIASYSLKNHSSTCSIKWIPESHYFFVGNDVGEVTLFETKAVVSYSIKQKVTFKCDQQQICGIDVNHLGKQLAIGANNNSSSIWDISDLCKPKKLFHLKHEAAVKAVAFCPWMPNLLATGGGSRDKNIRFWHSKSGTLISKHKTKGQITAVVWSRSKKELLVTFGFGDCSDKNSILAVYSYPTMKLKVKVNAPSDMRILTADLSNDFSSICTSISDQSVRIYNVWNSKFDLKSGIYDNGIYGSEIIDTEEGVDKAIDIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM68</accession>
    <name>A0A1E3NM68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit tim16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">Presequence translocated-associated motor subunit PAM16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_71334</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. May act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018352.1">
        <property type="nucleotide sequence ID" value="XM_019163895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM68"/>
    <dbReference type="STRING" id="763406.A0A1E3NM68"/>
    <dbReference type="GeneID" id="30180582"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="147" mass="15983" checksum="AB6122378B66A1EE" modified="2017-01-18" version="1">MAHRLLVQVIFTGARVFGRAFTEAYKEAAAATTVKQASADGKGPAKASAASRDGDIALDEACKILDVDLSGLTLDKAQKKYDYLFDINAKEKGGSFYLQSKVYRSMERIKNEMQYLEEFQKAKSKKESTGQGAAAGESNSNSHDKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM86</accession>
    <name>A0A1E3NM86_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_33760</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017811.1">
        <property type="nucleotide sequence ID" value="XM_019162842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM86"/>
    <dbReference type="STRING" id="763406.A0A1E3NM86"/>
    <dbReference type="GeneID" id="30179529"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13851">
        <property type="entry name" value="CuRO_1_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13877">
        <property type="entry name" value="CuRO_2_Fet3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000022">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000024">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044130">
        <property type="entry name" value="CuRO_2_Fet3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133436" evidence="11">
        <location>
            <begin position="20"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="30"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="159"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="368"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="592"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="642" mass="72612" checksum="38AC83E7616083D5" modified="2017-01-18" version="1">MIGVLLWCVVWLCTLGAEAAATTHEFYYKVQYVLANPDGVYEKQVISFNGTWPLPTIEVNKGDRVKLHLINGLEDATTSLHFHGLFMKGVGYMDGPVGVTQCPISPGDSMLYDFEVDQSGTYWYHSHSGSQYSDGLRGLFIIHDEEQEAKYAFDKQLSWSISDWYHLPSAELVKKQLTRYNPTGAEPIPQNLIFNDSRNVTINIDYDTKYLIRIANIGIMVSQFFSIPGYEFEIVEVDGVYTEPKKAKMAYLTVGQRMSILLKTRPKSDVDSNIIIFTSMDRPMLDTIPVDLELNSINYLVYDKALPMPKPPSWINDQDSFKPFNDLELVPSSHTPLLPEPDHRINLLLHMENLGDGVNYAFFNNYTYVAPKVPTLLTALSAPASLVNDARIYGSNTNSFVLKKDEVVEIVINNEDDNKHPMHLHGHQFQILARSKDFEEPVHYDPDKQASFAEFPIMRDTVYVEGNGYLAIRFVANNPGVWFFHCHLDFHLEQGLAVTLVEAPDYLDKYIPDDHIETCVRSNMPTKGNAAGNAHDFLNLHGEFVQPAPLPEGFTPKGYVALFACTAAALYGIWSIYVFGMNDISVTEAGKINNEKRVMQKYIEILERLKMKSVESQSEETSADIEKMLKQVIALNNKLSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMF0</accession>
    <name>A0A1E3NMF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_111098</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018425.1">
        <property type="nucleotide sequence ID" value="XM_019159753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NMF0"/>
    <dbReference type="GeneID" id="30176440"/>
    <dbReference type="OrthoDB" id="2015992at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="7"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="158"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="227" mass="24771" checksum="57DE378B2237DCD8" modified="2017-01-18" version="1">MSLQSLKNKTRKEVAARLRALDVAQVTAQSAATAAALARFPPFQSARSVGVYMHLPENELHTDAILDSCFRTGKTVFLPRIEQLHVFDEQPAFPGQKSCLHFLSVQSRAEADALPPRGKYQIREPDYAPDRSNDLLAGSDPLDLLLLPGVAFTATGRRLGHGAGFYDDFIKRYRAKHGTVPLLVGIGLPDQLVKDPSSLQLEPHDEPLDYVILGGSVFSAASSLPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NML4</accession>
    <name>A0A1E3NML4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018429.1">
        <property type="nucleotide sequence ID" value="XM_019163911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NML4"/>
    <dbReference type="STRING" id="763406.A0A1E3NML4"/>
    <dbReference type="GeneID" id="30180598"/>
    <dbReference type="OrthoDB" id="10571911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133440" evidence="2">
        <location>
            <begin position="21"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="349" mass="34847" checksum="4F7C81E1D363D7FA" modified="2017-01-18" version="1">MKLLSLLPLTLLSTLVSSAAIPEPQNFQYIAKRYVNESAVETLASDTDILTTLTETNSNGEDYTTLSTITSTIFVTQTKSSAAAANALTTAAASASSAVSPSASLSAVGSTGLADTLSQNKAAQIASATSGIPSSILQSLCPAPSTVTVTVTAGLSSSGSANIQLSEVTLLSTGVSTTTVTIPVTATFTISSGSSVSFYTASSMVATALEQTTIVTITHTNTLTSTAATSTGTFTGTAPAGGNAAANGGIAFNTDSTEYVTTTITSTAYSTRNPSSSLTEAAAAATTTPAATTSAPADTASAPADTTTGFPTVTSSDGSSGYHNGTYNVPTLKSESDTTTTLMKRGFLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMR6</accession>
    <name>A0A1E3NMR6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018525.1">
        <property type="nucleotide sequence ID" value="XM_019160791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMR6"/>
    <dbReference type="STRING" id="763406.A0A1E3NMR6"/>
    <dbReference type="GeneID" id="30177478"/>
    <dbReference type="OrthoDB" id="3997850at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="361" mass="39351" checksum="00306D76D22161FA" modified="2017-01-18" version="1">MNNLLESAADNIFITSANVLDTLTYRFSNTATKVTIPNNNDSLTAPLTEQLAKDYDTFNQVLNDYGMDLSNCVYILNSMKTKNQKEKVIKAKREQQRLEEERLRLIKEKEDMEKKERFAKENAQKQSAQLNGENGLTQVDSNGHNSSSNNNIDLFNMGSTPASTTNTNGRTSGGNSNGGNDGDDMMLDLDLDNFNFDGTGADMMNFDFNDPMSMGSSDKGNLQGVKSGMNTNRTSHNSQSSNTNANASGMANLNLSTNLNTNNNANTNQNANLNTSTNATSTNANSGANEYFNFGQDSDLADLNLDFLQDDEMGNLMQNMAVDGGGSGNSKNENEMNDDALAADQMEQLFSQFDEMVGNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN91</accession>
    <name>A0A1E3NN91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_31057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018687.1">
        <property type="nucleotide sequence ID" value="XM_019162673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN91"/>
    <dbReference type="STRING" id="763406.A0A1E3NN91"/>
    <dbReference type="GeneID" id="30179360"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF148">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE (CYTOCHROME)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="74"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="7">
        <location>
            <begin position="231"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="57792" checksum="49B27028B3F0622A" modified="2017-01-18" version="1">MLRKQLARTLNVRTPLGRSARVQRRTLSGTAKVDKASWSGYTYSFAALTAIVSSGVYWGAVSDTFSNESSAQTAGTVSVDEFVKHSSKSDCWVAVNGKVYDVTDFVPNHPGGQAPLLKHGGHDATMLYENLHPKGTIEKFLPPDKFKGVLDGEAPKLASDYAVDDDDEATRLEYIENKPPLSNMQNSYDFEQVAKNILPKDAWAYYSCGSDDEISMRENHYAYQRVYFRPREGVIQMISTLSSMSLKEIADARVPGATQWFQLYINEDRKVAREMVKEAEELGMKAIFITVDAPSLGNREKDKRMKFVNDTDVDLGDTVDRDSGASKALSSFIDCSVSWKDIKQVKSWTHLPVLIKGVQTVEDVIEAYDAGCQGVVLSNHGGRQLDTAPPPIELLAETVPVLKKLNKLRPDFEILVDGGVKRGTDILKAVAIGGKDVRVGVGIGRPFLYANSAYGEDGVRKLIQILKDELVMDMRLLGVTKIDDLTSRHVDTRRLIGREAINYLYDNVYTPIDAVKFSNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNA3</accession>
    <name>A0A1E3NNA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018688.1">
        <property type="nucleotide sequence ID" value="XM_019160855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNA3"/>
    <dbReference type="GeneID" id="30177542"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="168" mass="17065" checksum="FE349D40C52B33F0" modified="2017-01-18" version="1">MTAQQECSEVPAAAPGAVGTVVAVAGARWRLDTLVFSAVGTDRLDADLACVAVNAGTGSFARLTRTPAPDCSSLTGFFTRPRGPAAAAAAAAAGSLTSRWNTGDVSTSSVPRHPLGSSRLPKCRSWICSTLAGRSSGSVLPTTVSHASNTYSRRSPTLNCPASSAPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NND7</accession>
    <name>A0A1E3NND7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PBDC1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_32046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PBDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018719.1">
        <property type="nucleotide sequence ID" value="XM_019162738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NND7"/>
    <dbReference type="STRING" id="763406.A0A1E3NND7"/>
    <dbReference type="GeneID" id="30179425"/>
    <dbReference type="OrthoDB" id="10248897at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990593">
        <property type="term" value="F:nascent polypeptide-associated complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3560.10:FF:000001">
        <property type="entry name" value="Protein PBDC1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3560.10">
        <property type="entry name" value="yst0336 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023139">
        <property type="entry name" value="PBDC1-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008476">
        <property type="entry name" value="PBDC1_metazoa/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021148">
        <property type="entry name" value="Polysacc_synth_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410:SF9">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04669">
        <property type="entry name" value="PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysaccharide biosynthesis" evidence="5">
        <location>
            <begin position="18"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="147" mass="17517" checksum="447FEDD3C8BA0FA8" modified="2017-01-18" version="1">MSGLANFDPENPDNLEDIEKQFAVKAVQQMSTYWSLLSAVPPAKLKLTKYDDEILEEFYKTFPEYDEKKVAFLDEDELKSKEGKEKWREFIKNFEEKVEDYNFGTLVRKDVTKDYSEENTIFVVRIQFYAIELVRNKLGLNDWVYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNK2</accession>
    <name>A0A1E3NNK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018186.1">
        <property type="nucleotide sequence ID" value="XM_019160651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNK2"/>
    <dbReference type="GeneID" id="30177338"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="2">
        <location>
            <begin position="168"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="349" mass="39560" checksum="FA530B7155627837" modified="2017-01-18" version="1">MDETKLEVIDLLDSSEDNAKKIVHASSTQGFLMFEGHGFTQEEIDLLFQFSHDYFHLPVEEKQKCPIQQDNAGYTGMGVENLEEDDLDRGYGDPKEGFNFANFNLKTGIPDQPIPEFWKDKMDVVTATVLKLREALKRSLRLLAKGLEIEGVDGIDAEWFVERHHDDHFSGTTFRFLRYPSPVSANASTEEKAKFDEVNVAGAHTDYGTVTLLFQREQESGLQLFSPLSKKWENVPYVDASPKYKAKGEAAPLIVNIADQLCYWTNGLLKSTIHRVRFPKELLDQGKDRYSIVLFAHPGDTTLLEPVPSKRIREIKGRGASHYMDKHGVSQTAGDHLTKRLGSTYGWKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP04</accession>
    <name>A0A1E3NP04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018920.1">
        <property type="nucleotide sequence ID" value="XM_019164024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP04"/>
    <dbReference type="GeneID" id="30180711"/>
    <dbReference type="OrthoDB" id="3997099at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="37525" checksum="B1598B01F9C20173" modified="2017-01-18" version="1">MKDAVKLNDMIVDSYNSDTSVQALLSWISPANPAAPRAAPAATPPPVSKKHRKNPDYSMLSEDEIAEFRQTKELPLPESTQLLLPSIHIHARWYFNTNEYPPLKSKLDKRYMRTVFPSLIAHGKQLYYMNRLVSKLMKPPSHKMKRISGTGHWIYVINTPWNRDLRTEDFRFLGSTRKKYDELIIKIQECEHFRKRYEHVAVQEDKWEKLMGMNSATKASTDDWTWLYTEAEAVLNAEKTQLEQEVIQFCKRQGIIYEKIKPIFDEMHIHSKTNSEIMQKEVEIKEMGPYTDVLDGGLGLLLKKYGFRDPIESKYSKTPKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP18</accession>
    <name>A0A1E3NP18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UEV domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_119935</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018930.1">
        <property type="nucleotide sequence ID" value="XM_019159863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP18"/>
    <dbReference type="STRING" id="763406.A0A1E3NP18"/>
    <dbReference type="GeneID" id="30176550"/>
    <dbReference type="OrthoDB" id="306304at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072666">
        <property type="term" value="P:establishment of protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11685">
        <property type="entry name" value="UEV_TSG101-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052070">
        <property type="entry name" value="ESCRT-I_UEV_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017916">
        <property type="entry name" value="SB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008883">
        <property type="entry name" value="UEV_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306:SF3">
        <property type="entry name" value="TUMOR SUPPRESSOR PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306">
        <property type="entry name" value="TUMOR SUSCEPTIBILITY GENE 101 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05743">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09454">
        <property type="entry name" value="Vps23_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51322">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UEV" evidence="8">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="167"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="401" mass="43973" checksum="5B3DA2C58750929E" modified="2017-01-18" version="1">MQAVLNWLYAAVQPLYANPILTYQDVSLVLSVFQDVRIRTEVANGALLLKLYTVQDAADTADAAAGLRLLAAASPPLGVQVYLPRDYPQSPPVVLLAPADSKSHTLAASTYLTQAGTFTHPLLDAWTYTHANSRSNDGVPPRENRLLVLFYTLHRLIAEHPSIWVQRIPSLPPKPSRTLPAAPTRAPAPTTPAESPAVTSRVPPPLPPPPSSSSEKTTATASPASSSTALPPQLPPNPIRQALIRNASERLNAELLTLLNGSSQYSPLPPISTVLENQRKLVASYDTFNRTAKSIEGISTSLGTNTPVVELKSAEASALIEKVRNYVDSDYNHEENLLLPLDSRSKQLHDLCFREASQMDSLHFLELLFNTQKISFTDYVENVRKVSRERAKVLIWINKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPA6</accession>
    <name>A0A1E3NPA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_71941</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019047.1">
        <property type="nucleotide sequence ID" value="XM_019164055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPA6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPA6"/>
    <dbReference type="GeneID" id="30180742"/>
    <dbReference type="OrthoDB" id="10249039at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019788">
        <property type="term" value="F:NEDD8 transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="177" mass="20221" checksum="21131D352999C104" modified="2017-01-18" version="1">MQSVAFLRAKKDLQEYEEIPSVTITYERSNPMKVQLSIVPDSGYYVGGRFDFECTIPGEFPNAAPEFRCVQKIYHPNIDMEGHVCLNILRNDWKPTLTLQLVFAGILHLFLQPNPNDPLNKVAANDLAKYPDQFARHVQDAMCGGYIGSNKFDRVVAVKERSYGTGSRYNRAYNNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPF8</accession>
    <name>A0A1E3NPF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018546.1">
        <property type="nucleotide sequence ID" value="XM_019163939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPF8"/>
    <dbReference type="GeneID" id="30180626"/>
    <dbReference type="OrthoDB" id="3990582at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="788" mass="89587" checksum="7799CE4E98521746" modified="2017-01-18" version="1">MDSELNPHGQEMMRLDEIILKLSIHDPGSGTVNGDSDALLEDLRRYLSVYVDSYDTAVDERVRLLLSEQDLNIRDENKLELITIFSIIKTIKHIDSLLLELLNVESVRKINALLCSFFGLFRYHFQLPVHHNNYAFRNFGSLLTSPNINVLTPFITFMSRSSLSFSEVNKSETLEKTYCLLLVFHCFNKDLFKFLSKTLASKSNVADLMHHFSLTPITSKATEIPTLFTLYQRVRMSALLEESTLVVDTKSNNLMNILFYSLFHKQFLVHMITSIQVMHDFFHFPLPGNLFELSNKLLLDASLLDYLTIFVANDPGNIEALDNIVKANNSLENDRDVAVFLLLKSCQHHLLNYNLFASPDISLLSYLVEYNNANYASLLGITEGQNVCELNITALIPIFEIINQNVKLDYLKEREFLIVFKLALINIDLNLDSIEDFMNLNSLLIDIGSSLSIHALIDTIQTLLCIIINDVKFRHPTFSQIPKNFQNILGFQFIPPVYRSDMSFENNIDLQSEFFGPMFTSLQELENYPGLVATASEILLEDCLTIILSIKSKVFRFLKNLNNETNVLRLPDLFSDDNKNEEYPLHVFKQGISTKIGSFKSSKSLNYKLLNSSISLGIVANLSLLVVGDNYKSLRSSDFMIDNSLSRLLNEFTVDCTLTFLIIYQEFGLLSLFKKIRNLNNENLKMVAPSASLLSKVFQAKSTDSKSEINSVIQTSSFEIASEILNKSVISSNLLRQYVELFDDGQSKPFKSLNKFLKSHPSTLKPSKISKGTVILDLAEYKTALAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPN1</accession>
    <name>A0A1E3NPN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Nuclear transport factor 2</fullName>
            <shortName evidence="3">NTF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15442</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018616.1">
        <property type="nucleotide sequence ID" value="XM_019160827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPN1"/>
    <dbReference type="GeneID" id="30177514"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="4">
        <location>
            <begin position="8"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="124" mass="14315" checksum="62F6D7BCE121F4A8" modified="2017-01-18" version="1">MSVDFNTLSQQFCDFYYKQFDQDRSSLGNLYRDHSMLTFESSQVQGARSIIEKLTSLPFQKVQHRISTLDAQPASENGDVLVMVTGELLIDEEQNAQRYSQVFHLIPDSGSYYVLNDIFRLNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPY9</accession>
    <name>A0A1E3NPY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15983</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM170 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019282.1">
        <property type="nucleotide sequence ID" value="XM_019161043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPY9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPY9"/>
    <dbReference type="GeneID" id="30177730"/>
    <dbReference type="OrthoDB" id="2131401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019334">
        <property type="entry name" value="TMEM170A/B/YPR153W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779:SF6">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10190">
        <property type="entry name" value="Tmemb_170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="188" mass="21590" checksum="5F81AC26D71D66FA" modified="2017-01-18" version="1">MVAAPIGYETPTFPSLYWPLGQYSDDFHSSFLYHTKDIWEFTTVWTIIFMTGVYTASACIFLVTHYIGSSRHPTLKNEGDEIEMRNHNFALHEMNYNHIYQTTMSTYQHYFVTSLFTGNRKILIYTVFAYILLGAIQGFIAGSVVGVLIAAIYDSAQFKVTTWIPFVYSLIITLYNIADSYSFTVKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPZ6</accession>
    <name>A0A1E3NPZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_58461</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019290.1">
        <property type="nucleotide sequence ID" value="XM_019163301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPZ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NPZ6"/>
    <dbReference type="GeneID" id="30179988"/>
    <dbReference type="OrthoDB" id="3997753at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="284"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="371"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="567"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="672" mass="74618" checksum="848B9EBFA0B33FEA" modified="2017-01-18" version="1">MEAAHTIAMYDDNSSGIARAGLRQTDLSVQLENSNKKKHRNKTQSVLYNGAMAKSKSSLFDGTSLFSEVSSGDYQSSNNQASQQQQQQQQQQQQQQMMMANQQMAMAQQGYAGYPQVQYNAQMYSSSNHSNPSFQQGYYPQQGYYGQGQYPNYQQQQQQNYLGANQAYPGYQYAPQQQQQQQQGVRVAKQRKGKKGFRESYMYLNSVNTSGELINMAQQPQVNMPVANGSPTPSLQRRFSAQNGGVVGSPMSMTNDENARKLAFPTIMNGMGHALNVNDGDPANYGTTVNDWESIDEDDDDEEDYEDEDEDEDVDESEYDSLSKAELQKILLSVKGENKGLKKKLKDIELGHDSSSIVANDEKYIKLEEEFSDLATKYVSIESEVENLTNENETHLKDIALLKAELKQSEAAADRCTTLSRTMIERIPNFVKQKDSFFLIMGDASEIPEQLQECYTRSVEKYQGEDSFENPADGNLSDSDIMKMLKNEVRDLQAALTRSENTVKQLDKIYSQKLHEERRTIIMSSLQKTLHNEFVSDHSNAGARAGLKHFRIVDICPSAEPQGGFLSPSIPNSASSPSLNNNWSSSSGSSSLTPDHSHSRVVYLSPISPPSSSGGSPTKRISLYSGNSVDHFKDAFSTIPNSSSNPTIAPGPAAAANSFDTSLEIEYETFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPZ8</accession>
    <name>A0A1E3NPZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_58424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019235.1">
        <property type="nucleotide sequence ID" value="XM_019163298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPZ8"/>
    <dbReference type="GeneID" id="30179985"/>
    <dbReference type="OrthoDB" id="3998037at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="531" mass="61578" checksum="B0FC6E1C41483750" modified="2017-01-18" version="1">MLSRAFLKEVRVFVERREPQTAILQSSASLSPSPFEEWNFKQFIGDKLERQVERQKKLPTLNALVENRLEQLLPLYAHLLDTGKDDSVHGHMLKFMGRGSGSGDGSRDAKLKNLKAHIIKYEIHREYFEVSEFRRSQLSITVDPTVSSEMLVHTYTDKDLVHFVKLLHDETFLNSSYYNYYRRFSRLFFNLVEGSNEPGSNARLSRKLRPADLERLLQHLLYSNKRLRTEERLELRLDEMNNILLNDLQEAGLKLSNKEMFALLQLTFRKTVDLRNVEMLYDKFMASDKFDKNNDFFKTFLNFNMSLGFSNERMASTKFCRKVILDLVQSDGVDVDRHLLKYLLKYSWLVHDGELTRLVATYLLKHYALDVETFNMIFVGVFRQDMSSNTVSPALEQLLNINLKHTMRVSSLATSSSVASRIAPPPITEAERFNMRLLDHLLEKAARVTPQMQPLCPGIMHRLPLNLVPHDLLLRTTQNNTGDGFPVVEDYLRQMSRVASEGPEGLQHRLRHRNQGGRTQRPTLHLDPGHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ06</accession>
    <name>A0A1E3NQ06_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_124633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019302.1">
        <property type="nucleotide sequence ID" value="XM_019159929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ06"/>
    <dbReference type="GeneID" id="30176616"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="79" mass="8921" checksum="3EBF3EDB015DC5BE" modified="2017-01-18" version="1">MRHGMRRPKRDTALPRDGKSEPQVSQPRRVETADWAVSGSDLRRSGQWGGSRDWEALRLGESNLFTGRALDISENALFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQC2</accession>
    <name>A0A1E3NQC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019394.1">
        <property type="nucleotide sequence ID" value="XM_019161083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQC2"/>
    <dbReference type="GeneID" id="30177770"/>
    <dbReference type="OrthoDB" id="10468155at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="537" mass="58027" checksum="0A7E774CAAFABFA9" modified="2017-01-18" version="1">MEHGTTVKTKIHSVPSVDGNSPANMNASSGFGSAANPNPNPNMNSASTSNTSTSASTTLNSGMNLNASTGATAVAHSTASSNSYPSSPAAQSTNRGKKFSWVKRLMNNNNVIAAEPTPLAHTRGNRQHRQNDGQEQPYRQTRPRAQKKGRKDAAETSKLKPKPAADKDADRDNTVLRKPAVYEHNAGKIFQFNDVTSIENDEMVSYVSSNASLKLQDSYDRQFYMRQDGYDANTIASVKSIFSVNPAPSIYSTGAVSTNTSGTNNNNSSSNRSSFAPAQLSHINHMHPVAHSHGHNYNHLNTNPNPNTHNHSTPLLHQNNGTSYNNYLDSASAFSGSVAEDNVSTKPLVSFSSDEEEDCDNDGSQLDYSDDFHAHKPKHASGHHVQIADDDRNTRTWKNSDANNSEEFLAESITTTGTNGDDKGGGGGDHVDDDDTDRTHRASNIDRYIQLNDESKEHYKRHDDSDQSSFDVKSKYPSSTKSAAVTSMSILTSPMLTTINSSTTTATTMTNNTTNTNVATAASVMTLASSSRHVYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQS3</accession>
    <name>A0A1E3NQS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_19734</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019519.1">
        <property type="nucleotide sequence ID" value="XM_019162137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQS3"/>
    <dbReference type="GeneID" id="30178824"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044843">
        <property type="term" value="P:cell cycle G1/S phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20559">
        <property type="entry name" value="CYCLIN_ScCLN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000010">
        <property type="entry name" value="G1/S-specific cyclin Cln1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="74"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="172"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="326" mass="37367" checksum="1D3890DD7A08150E" modified="2017-01-18" version="1" fragment="single">HSRKSYGPPRRHMCKPYSAKLEAIENYKNKIISAEYLDTISAHYKSLESESAVNPAMIDLQPEVKWFMRPYLVNFIVQMHSSLKLKPQTLFLCWNIIDRYCARRIAFKQHYQLIGCTALWIASKYEDKKSRVPTVKELNTMCSNVYDDSMFREMEIHILSTLNWSIGHTTLEDILQLAVKFSDPDGKELLDKPIDLYKGNTPTVSAILAVSRYLCELTLYDRVYLTFPTSTIGISCFLMACSILNMDVGSTYIRETDDDAAYDSDAENTEPKVHEQGPFISGFNGMDSITQVREIALHLFRSMLDPSEVLIDKYSSLGVMTVVKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRL6</accession>
    <name>A0A1E3NRL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_14244</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019827.1">
        <property type="nucleotide sequence ID" value="XM_019160289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRL6"/>
    <dbReference type="STRING" id="763406.A0A1E3NRL6"/>
    <dbReference type="GeneID" id="30176976"/>
    <dbReference type="OrthoDB" id="10260897at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="71"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="414"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="572"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="653"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1144"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="426"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="575"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="600"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="660"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="717"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1145"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1206" mass="139069" checksum="4FD940219BA0052D" modified="2017-01-18" version="1">MDSQQSNKTHRGGGAKKNTAKQKLHSQGKNAKAFAFAKPGKLQRQATRSSDLGEKKYHVPMVDRTPDDDPPPVIVAVVGPPGTGKTTLIRSLVRRLTKTTITDIKGPITVVSGKRRRLTFIECSNDLNSMIDIAKIADLVLLLMDGNFGFEMETMEFLNIAQHHGMPRVLGVTTHLDLFKKPAIMRAAKKRLKQRFWTEVYQGAKLFYLSGVMNGRYPDREILNLSRFISVMKFRPLKWRNEHPYMMSDRVVDLTHPSKIEKDPKCDRKIALYGYLHGTPLPNENARVHLAGVGDYTVSSIEKLPDPCPTPYYEQKLEDYEREQRKLAEANGQPVPAKTHRRRKRLEDKQKIIYAPMSDVHGVLIDKDAVYIDVGQKVFKSEEEMSGIGEKLVTDLQNAADIVDSRKSSRLKLFSDGPEISRTDSDDNDELSEPDENEKDDNEELHGEMGRHELRRARIHKQDLENLDDNADIDEYDEDEDNLVIFDEDADDRAREMKLKQLRKSQNLANEEDLQFETDSELGDDDDEDNGLSQWKAMGGKLQNLNIKKTWNINKLIYMNNIDPKDAIARWKQEEESDEENITPSSDEGESGEEENDEEFFTKKEDKMIEETNDKSKLTLSFLKEKWAPGSKSLDYLKKRFFLSAKQRIQMEREAKENGEENELYGDFEDLEDEDAEEKEYIEAGDEPDGEMSEKDDFTNFEEEEMKDEMGIGTKIEEEEEGDEEDADDDDSDADQSVEKQRALNAKKKEKLKQQFEEEEDYKGDDDPFNEHDSWYEFQKAKMAKQLEINKAEMSKLDADTRNKIEGFSAGSYVKLTFDSLPMEFIENFDPTFPIIVGGLLNTEERFGFMNARIRRHRWHRKILKSNDPLILSLGWRRFQTLPIYTTSDSRTRNRMLKYTPEHAYCFTTFYGPLVAPNTTFCGIQVVDKDHTTGSFRIAATGIVEDLNAESSIVKKLKLVGYPQKIYRNTAFIKDMFTSAMEVAKFEGASIKTVSGIRGEIKRALSSPEGHFRATFEDRILMSDIVILRTWYPVKPKKFYNPVTSLLLKDKNNWKGMRLTGEVRAVSGVETPQGKDSNYKKIERQERKFHSLKVPKTIKSSLPFKSQIQDMKKQKKPTYLQKRAVVMDGKEKEARKLIQTIQTIRNDKESKRTSTKDSKMKEKLKSREKLEAFRTEKQKEKKKEYFETHGKKRKLDSTDGGSSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRX7</accession>
    <name>A0A1E3NRX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70438</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019953.1">
        <property type="nucleotide sequence ID" value="XM_019163642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRX7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRX7"/>
    <dbReference type="GeneID" id="30180329"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="5">
        <location>
            <begin position="90"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="917" mass="105066" checksum="CA6A6B39E839E705" modified="2017-01-18" version="1">MINLDKAGDRALLMDFYQLKTLNPKDYQHDSIQSTFKVDLSTEDLSQIPKEVVNHILGDNMNDLNSSHTKERPGSIKRHPSTNAKSNYADALGFHQNILDELIDKDIVDDTRDPQLNRYIIDSKLFNPKLFLSVIHSDKSLNDLMLGIHNLENDIEAKKPLLQQLITNNFEKTLSSKNSLDKVFNEFSSSNLGQEIDNLNQNLAYSNNSANQLLNPVLLLISKEQELANALTFVNQNKFFLDLPKKLKSYIDEDDFDSLLKEYERGFALFQSLKTLQNHNPLFDKIWSNVVKVINDYKISMTDDLKKIHIESINSNFKLQANNSRKSNFISIIKRMIELNATRSPIKDFISLQYDYIMEDLDTGLAKINYTRLFNARNSILNAYQSNQDPDSEILLVTTMKRLFTTFGSSTFNDEQIDKIYEKLDFPLIVQLWTFTSDYINDATELVIGKKMLKFESIVEYFLTDFDSLLSKKAKSNPNSFKTNDKDLALFKNYFSSMVSKICTRLQFFFSCTTSDLTNALQLSMDQGDTAALPPSGKPDLNDPSTFGFLPPNTNSISAICFSVNLHDMLYKKLSEVQNKALLLNSGELNSIVTSTILIINQNLILGCLSTLNADIRKIFYADNMSPSDTIDGATKLITFLQNYYKVFISKLHQIHIFDNKDLMEVIKQQFLSSFDVLLNGMVENVARQSRIDPTRSDYYYLATIYDMRNLTQKIIPSILRSFDINFQSNLSKDKDLKIYKSFDSYEYALFAEYMKEPLTAIKQLVGNGITEINLNSEGLLTRLASGEVIEVSPYILKAINHINNLKSRLLNFKIRNSFILDVQSSLIGQFQKKIVDNLSIDFTEDGLYQIVLDINLLLVLLKKYNTRCPEINIADTKRLEQVYQKLKLKADISSVEKNREANINFNYAQFVCFITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS02</accession>
    <name>A0A1E3NS02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70111</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytoskeleton</location>
            <location evidence="7">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019591.1">
        <property type="nucleotide sequence ID" value="XM_019163556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS02"/>
    <dbReference type="STRING" id="763406.A0A1E3NS02"/>
    <dbReference type="GeneID" id="30180243"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF2">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="362" mass="39848" checksum="A82DF473FB222FE1" modified="2017-01-18" version="1">MSIPFVLSKTPIYDHCFSADRRNVAITKDKDVQIFNLSNPSKPQLITTLKHHDKPVTAVDISADGKIVTCSQDRNALVWTPQADGSYKETLVLLRINRAATCVKWCPNGAKFAVGSSARVISVCYFEEENDWWIAKHIKRPIKSTVLSLDWHENSVLLAAGSTDGHLRVFSTFVKGVDSKPAATIWGERLPFQTLCLDVNLNAWVQDVKFSPDLQFVAGVGHDGSVFVVYPGAGELEVSNVVKVKTSNLPFTSLVFVNPSKLVCAGYDCHPIVFEGSGSQGWKQTYAIDDPKKKKANVVENQSALNMFRQMDLKGSSEPKSNTLLTVHQNTITSLRPFQSEASGLIRFSSSGNDGKVVIFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS06</accession>
    <name>A0A1E3NS06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="5 14">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_70120</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="5 14"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019601.1">
        <property type="nucleotide sequence ID" value="XM_019163562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS06"/>
    <dbReference type="STRING" id="763406.A0A1E3NS06"/>
    <dbReference type="GeneID" id="30180249"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000139">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000017">
        <property type="entry name" value="Protein disulfide-isomerase A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11 12" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027326" description="Protein disulfide-isomerase" evidence="14">
        <location>
            <begin position="24"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="22"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="319"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="491"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="408"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ48488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="530" mass="58422" checksum="5DFAA6391E5081AB" modified="2017-01-18" version="1">MQFSIKAVAAILAAITNIASVSAEGATAPEDSAVVKLTEATFKDFIANNPYVLAEFFAPWCGHCKKLGPEFASAADSLVETNPDIKLAQIDCTEDRDLCAQFDIRGYPTLKVFKGEAAPPSDYAGQRQSDSIINYMVKLTLPAVQVFDDTAVLEKTVEALAEPLVLQVLPEGVEKSSSNETFYQVADKLRESFTFGTTSDPKYVAEYAKSAKKPAYVIFRNGEDAEDASVYKGKDIDEESELLIDFIDVESKPLFGEITGATYQSYTSANIPLAYYFYSTAEERDAADPFIKKLARKYRGEINFVGLDASQFGMHAQNLNMKEEFPLFVVHDLEGNKKYGIDQSTPLDNNAIAQFVQKFKAGKLEPIVKSEPIPEVQNSTVYHLVGAEHEKVVKSGKDVFVKYYAPWCGHCKRLAPIYEELADLYEGKDVVIAEIDHTLNDVESVDIKGYPTLVLFPADGSEPIYYEDARSLEAMANFIKESGSLKIDALVDEEDEEEVKETAAAETISETTEKAETTAPAAEADAHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS21</accession>
    <name>A0A1E3NS21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019621.1">
        <property type="nucleotide sequence ID" value="XM_019160208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS21"/>
    <dbReference type="STRING" id="763406.A0A1E3NS21"/>
    <dbReference type="GeneID" id="30176895"/>
    <dbReference type="OrthoDB" id="4521223at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF1">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="307" mass="34533" checksum="1706041509C50011" modified="2017-01-18" version="1">MAYVPIAQISTAGNAVMCAFFGIQCLIHVFLGIDYRRWRFLIFMFSGTALSCVGYAGRAWYSRDAATSPYIMQAVCVAIGPGLVTGAVKDLFVHFVSYMRMKKIDQEPNWYMDSGIEKFGYYEKLNRFITLASSVLIGVGIGVLSGSDLNDSKMKQSIQVALAGFALQLAVLGITLILWSFFIYHTMGQPYMHGRVKTYVFSLFVVLLLLIIRFSYRVAEWAKILKLGLANNLTINEDYLVCLDGLTTLLAGMILIVEHPGFVFGKAELKQIDDEFHEYEKQNKAAASPTVQNLQILRYVPLFHLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSB4</accession>
    <name>A0A1E3NSB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone deacetylase complex subunit SAP30 Sin3 binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020009.1">
        <property type="nucleotide sequence ID" value="XM_019163654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSB4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSB4"/>
    <dbReference type="GeneID" id="30180341"/>
    <dbReference type="OrthoDB" id="510958at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 Sin3 binding" evidence="1">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="150" mass="18009" checksum="16123AF3EE27F66B" modified="2017-01-18" version="1">MSTRSSQKQKPPYVVPVQKEYVTKYVHDNHPDNQPAKDSVDFSNFSADELREYRMRFLNPAHQLSPDVLTFQGYLLENNNLGRRTESSRMNEQRKQENNLYDYRTMDDLRTNVENHFKEQLPVKESEVIMDFVYKVRKSDDKFRIHFDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSB8</accession>
    <name>A0A1E3NSB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_57092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020063.1">
        <property type="nucleotide sequence ID" value="XM_019163212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSB8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSB8"/>
    <dbReference type="GeneID" id="30179899"/>
    <dbReference type="OrthoDB" id="3998031at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133491" description="Extracellular membrane protein CFEM domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22184" checksum="718331FBDCFB8610" modified="2017-01-18" version="1">MKFELYLYVLLVVFGGAVVNASSPPAAVLHCVNDIARLCEGFHSNYECICYNRDRMLYCLATVSPYGNYMEARDHFLGTCIDRIPALINDPNFNLDLPRKTLTTTVGGTLTSASEFPSSALEPSNGTKTPLPTLEPTASATEPSVSEGYPSTSVHTSEHTTTSTDLSSEYHSPVSTEPLPIPLPTDIPVHIPPDECDDNFEDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSH6</accession>
    <name>A0A1E3NSH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3533 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70629</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020164.1">
        <property type="nucleotide sequence ID" value="XM_019163693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSH6"/>
    <dbReference type="STRING" id="763406.A0A1E3NSH6"/>
    <dbReference type="GeneID" id="30180380"/>
    <dbReference type="OrthoDB" id="2140105at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022703">
        <property type="entry name" value="DUF3533"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053001">
        <property type="entry name" value="MNNG_permease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814:SF1">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12051">
        <property type="entry name" value="DUF3533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3533" evidence="3">
        <location>
            <begin position="100"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="465" mass="53123" checksum="9E3280BA2B11DD8E" modified="2017-01-18" version="1">MSDKQSNESSSSEGVQPYVREGRQESVHSGEEEQGLLRTISEYNDETVTSGEARENERVREEDLEKGIKPPGPPPKYPLFSKENKGMRMKVIKQYVVVIVALWVFILSVWPIYWGSMYKRDGRLVNLHYLVAVEEDRAAPISQALYLATQHPDMQKLATWNFESNLSEADVQKMIHDQKYWAAIYVTQNNVSQSLTDAFTSAENIDTRDFVRSLYETARDPSTMEGIVSPTVFRFESYFQEALQTEIYPPLIKNLTSEQFAKLQNTNVLTSYPIIQSTDAIPVEPVTNGPLQVGLIYIIIITFFEVMWMAQLYGTVAKSAITIHYILFRMLLSQLNYLLISLAFTCLNAAFQIKMDNSWSGGFGVFWMVSYLTMAAVGGANQNVGLILFAFYRFTYAMPIKNAYELMKVTLFDTSRKDLGRYFGILVAWIVLNNILLPLCMAFFSWTMKKKIMKEIKSKNAAKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSM1</accession>
    <name>A0A1E3NSM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_18969</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020212.1">
        <property type="nucleotide sequence ID" value="XM_019162096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSM1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSM1"/>
    <dbReference type="GeneID" id="30178783"/>
    <dbReference type="OrthoDB" id="2155283at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050897">
        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF5">
        <property type="entry name" value="PROTEIN SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SAM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="55" mass="6258" checksum="DA76975927991450" modified="2017-01-18" version="1" fragment="single">LLADIPAWLKTLRLHKYTENLQHLRWQDMVALDDAALAQLGVSTLGARNKLLKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT44</accession>
    <name>A0A1E3NT44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_70832</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020392.1">
        <property type="nucleotide sequence ID" value="XM_019163752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT44"/>
    <dbReference type="STRING" id="763406.A0A1E3NT44"/>
    <dbReference type="GeneID" id="30180439"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="3"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="189" mass="21252" checksum="44A719A02FE76D5E" modified="2017-01-18" version="1">MAGRPIVISGPSGTGKSTLLKRLMTEYPNTFGFSISNTTRSPRPGEKDAIDYYFVTVEKFKNMINNKEFIEWAQFSGNYYGTSIAAVKHVSESLKRTCILDIDMQGVKSVKKTDLNARYLFLSPPSIEELKKRLEGRGTETPESLAKRVGAASAEMEYAKTGAHDKIIINDDLEKTYKEFKEFIFSEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTG1</accession>
    <name>A0A1E3NTG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_61296</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019956.1">
        <property type="nucleotide sequence ID" value="XM_019163462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTG1"/>
    <dbReference type="GeneID" id="30180149"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="51"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1150" mass="130506" checksum="BD2A3A42885D3502" modified="2017-01-18" version="1">MSEGMAIMSPQVELTAEVTNATDKDLELNHDSNNSDKISKPKSRRMRMPLSCIVCRKRKVKCDRGRPHCKTCEKYNSVNLCSYVEPTWTNTDGKKVTFVNHMGVMDPNQAEKSLSETDITFTHNSTSIAPKISLDARSVYTDETISGSSDKTVDASTSGRMGLNNGFFTNDNVSNSPQFNDRLANPTFYGSSSDIPSSISSGISPFNKPLLPPLESLPANVTGVAGISLQPLIQQNPQAQMQRKADLQVLLNKIKKSDVNVNNHDCLKKVDTSTLEKSTMNWKPRDQMGVSPETVASNSPGNVLDKGQPSWLKSSNMNCINSSNESIIQGHSSSDKPTIATPNRSRSLDSSYIALFESLGIGVDESYNLYEGFVPMLYKFSRINNFGPLAWISSILKDPFICPLRDEIVQHKKKSLFKATSEGETDHQNRFLRYSGIDDIKPIHIEVQSTTATDNDSDAKLVKEGMLNKPYDGTDINTHINPKCVYFNSNFALQQILDVLPEKKVIWLSIKRFFTYVYPILPYLDQLSFITDVEKLIDGNHFQDRYSEEKIGTIHVTKKLDFANLGILLLVLKFSEASLIVNEDLGDGSITRTKDEQYLLDHPLNEKMINVAQSCLNQFRLLRRCALPIFQLALLMNEYEKLNGLADGNDPDSQIFIAMLIQMAISIGMNRDPSKFDTFIGKGKVGNLWRKIWYGLLSSDVKQYIQFGSGKSAMCDFYDTELPVFDEESSNIDNYDLERVVIEKIRLNFKFDQAMLELADYLCQFKKNPNVRVVLTKVFNLEAMMKESFGTMKDLLNKSSDLFIEKVRKVWDFTIYVLAVGLLVTVYHQLFVNYQKLESYAVTKFVKEKSEVHWLYVFSNFKNISSKSSKYFGIGFDMVISQCIISTVHKGWIDFMSTYICLRLAVSKIEDKANSSKRTHLLRKVCDLIVDSENWYLPSLKILSRRHFHAWKLLKAHTFLIGLIRKNQLMFKPLSHLYNYAEYMSDADLMSLLEMTRYRNYMAEENESKVFTAIKNRILDNCTSLSNIPTSDSPNSDTVNTTDGNNSGHDIAIDGLFPIEAWNKPMEEDTFWRDIFFQKQQQTFNGPTPTASPGFDFLAPQMLSQDIFPEEDYEINDPFEGQEVGPDGVIPNSSNPTNLFVDQTIYNMFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTI2</accession>
    <name>A0A1E3NTI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020477.1">
        <property type="nucleotide sequence ID" value="XM_019163769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTI2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTI2"/>
    <dbReference type="GeneID" id="30180456"/>
    <dbReference type="OrthoDB" id="10262413at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF6">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="336" mass="37832" checksum="10CD4FD01CDEDDFC" modified="2017-01-18" version="1">MKLFITGATGYIGGEVLHQFLQKLPHFWVTALHRLPEEAKLLKDSYGDRVHPIFGSLDNSGTLEREAREADIIINAACYNHAPSVEVFKKVLSERRTKALFIQISGTGILVDSTEPDGYRPDKVYSDVDDIDEINSLPDSQPHRPAEKVALSIEDSNPEFVKTAVVCPPLIFGIGQGCGNKLSVQIPWLLRAVVEVGYNFTVYDGMTRWDHAHIYDVGTLFMAIVEKYLQGEPFRSGHAGYYFVEDGSDFYWKDLAAKVSDSLYSRGLIRSREIKKLNPAQVREIFHLPAMYWGSNCRSKADAGKLLGWRPIKSDDAHFWADVDESLDYMVSRGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTS2</accession>
    <name>A0A1E3NTS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71027</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="6"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020595.1">
        <property type="nucleotide sequence ID" value="XM_019163806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTS2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTS2"/>
    <dbReference type="GeneID" id="30180493"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016880">
        <property type="term" value="F:acid-ammonia (or amide) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF245">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="96"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="545"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="673" mass="73536" checksum="18DB242FA02371E4" modified="2017-01-18" version="1">MTTTHSVVHEANGVVARPTPQPFFEKQPAKSLSSKTEYEAMYKQSIEDPEGFFGPMAKKFLSWDRDFTKVKSGTLENGDAAWFLGGELNACYNCVDRHAFATPDKTAIIFEADDESESKEISFSELLKEVSQVAGVLQSWGIQKGDTVAIYLPMTPEAIIAMLACARLGAVHSVIFAGFSAGSIKERVTDANSKVLITTDEGKRGGKTINNKKIVDEALKDCPSIEKVLVYKRTGNSAISMQAGRDFYWQDVAAEFRGYLPPVSVNSEDPLFMLYTSGSTGAPKGVVHSTGGYLLGAAMTTRYVFDLQPEDVLFTAGDVGWITGHSYALYGPLALGVSTIIFEGTPAYPNFGRFWRIIERNKATHFYVAPTALRMLKKSGEDEIAKYDLSSLRTLGSVGEPIASEIWQWYDEKIGHGCCHISDTYWQTESGSHLIAPLAGAVPTKPGAATLPFFGIDAVIIDPTTGKVLEGNDVEGVLAVKSTWPSMARTVYKNHKRFMETYLKPYPGYYFTGDGAGRDHDGYYWIRGRVDDVVNVSGHRLSTAEIEAVLLEDSARVSEAAVVGINDDITGQAVIAFVQLKDNTVVNDDATLLKLRQDLRLSIRATIGPFAAPKTIIIVDDLPKTRSGKIVRRVLRKIVSGEAKALGDQSTLADPECIPQIVEAVEKQFGVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTT0</accession>
    <name>A0A1E3NTT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit TIM44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_14936</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim44 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020605.1">
        <property type="nucleotide sequence ID" value="XM_019160602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTT0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTT0"/>
    <dbReference type="GeneID" id="30177289"/>
    <dbReference type="OrthoDB" id="10265990at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.240:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017303">
        <property type="entry name" value="Tim44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037871">
        <property type="entry name" value="TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim44-like" evidence="13">
        <location>
            <begin position="246"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="407" mass="45706" checksum="841AA715493A3E76" modified="2017-01-18" version="1">MYRQITVRNSLMLLGSRSFSSARTVLAPKSPMQVFMDTFKKEWKKSSELTDQIKQLKSATDELGDSEAFKRAKEAYATAQKGSGKLSQGVQSTAKVVGDVAYKAWESPVGQAVKKTAETTADVVDKVVEPVTKTKAYKDVSEVFNEGSSSYGLYETKEERLKRREKELAKKPKTVKRNEEAGSALVATDIKPTSSFKENLKIEPNSAIGKFFLILREKWEEAENPLLVLIRTILNKIGGLFAETEAARVVKQFRDLDPNFTSIAFQKQLREYIVPEVVEAYIQGDEKVLKSWLSEAPFNIYAAKQKQLRDQGIFSDGKILDIRGVDIAAYKILEPNNIPVMVIGARVQEINLYRKFKTGELVAGSDEDILLSSYAMVVTRVPEEMDNPETEGWKVLEFVRGGSRQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTW2</accession>
    <name>A0A1E3NTW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_14498</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020106.1">
        <property type="nucleotide sequence ID" value="XM_019160403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTW2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTW2"/>
    <dbReference type="GeneID" id="30177090"/>
    <dbReference type="OrthoDB" id="10254238at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="23"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="130" mass="14079" checksum="BC18E48B90436894" modified="2017-01-18" version="1">MAPKADKVASKAPAEKKPAGKKTAAATETKKRSKTRKETYSSYIYKVLKQTHPDTGISQKAMSIMNSFVNDIFERIASEASKLAAYNKKSTISAREIQTAVRLILPGELSKHAVSEGTRAVTKYTSAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV17</accession>
    <name>A0A1E3NV17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_71054</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020621.1">
        <property type="nucleotide sequence ID" value="XM_019163810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV17"/>
    <dbReference type="STRING" id="763406.A0A1E3NV17"/>
    <dbReference type="GeneID" id="30180497"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="17"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="359" mass="39315" checksum="112283F359E74FB8" modified="2017-01-18" version="1">MPSITNNNPCVVLQKVGSITYEDRPVPELTDPHYVKIAIKQTGLCGSDLHYYETGCCGSFKVEKPMVLGHESSGEIVEVGSAVTSLKVGDRVACEPGIPSRYSQEYKSGNYNLCPHMAFAATPPYDGTLCRYYLLPEDFCVKLPDHVSLEEGALVEPLAVGVHANRLIDVKFGDSIIVFGAGPVGLLCAGVAKAFGCDKVLLVDIIDEKLAFAVEHGLASHTFNSRGQKFDDLMEKVKSIWKDSEHGLPTCGIDATGNQTCTNMCIRALAKKGRFVQVGMGPDNMDNFPIAAVMEKELTVKGSFRYSVNDYNYAVQLLKERKLNVKPLVTHRFKFSEAIEAYEFSKKGQSIKIMISGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV18</accession>
    <name>A0A1E3NV18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_57440</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020506.1">
        <property type="nucleotide sequence ID" value="XM_019163238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV18"/>
    <dbReference type="STRING" id="763406.A0A1E3NV18"/>
    <dbReference type="GeneID" id="30179925"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060258">
        <property type="term" value="P:negative regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000394">
        <property type="entry name" value="Serine/threonine-protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="32"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ49393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="388" mass="44003" checksum="E353B0E642C79E67" modified="2017-01-18" version="1">MSHISDKENRQPYHPSFAAKAAKAAVTRVGPWRLGRTLGKGSTGRVRLAKHTTTGQLAAIKIVPKNIIDFDGSNSASNNNQRKKKRKQKVDENGLPYGIEREIIIMKLISHPNIMALYDVWENKNELYLVLEYVEGGELFDFLISNGRLGEQDAVKYFRMIINGVSYCHKFNICHRDLKPENILLDKNGKIKIADFGMAALETQQKLLETSCGSPHYASPEIVAGKTYHGSPSDVWSCGVILFALLTGHLPFDDPNIRQLLLKVQTGKFHMPSNLSDEAKDLIWSMLRVNPNERISIDKISQHPLMLRYPDNAVDEVEDKLDHLDISKPVSNFDPDILNNLQTLWHGISQSQIVLKLQNKEKNSEKIHQYQDLVVPLSPQSRMKMEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVK0</accession>
    <name>A0A1E3NVK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71116</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ELP1/IKA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020686.1">
        <property type="nucleotide sequence ID" value="XM_019163833.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NVK0"/>
    <dbReference type="GeneID" id="30180520"/>
    <dbReference type="OrthoDB" id="40048at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056167">
        <property type="entry name" value="A-sol_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056164">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056165">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006849">
        <property type="entry name" value="Elp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056169">
        <property type="entry name" value="HB_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056166">
        <property type="entry name" value="TPR_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23925">
        <property type="entry name" value="A-sol_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04762">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23797">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23936">
        <property type="entry name" value="HB_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23878">
        <property type="entry name" value="TPR_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017233">
        <property type="entry name" value="IKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ELP1 first N-terminal beta-propeller" evidence="7">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 N-terminal second beta-propeller" evidence="8">
        <location>
            <begin position="422"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 alpha-solenoid" evidence="10">
        <location>
            <begin position="727"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 TPR" evidence="9">
        <location>
            <begin position="946"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 three-helical bundle" evidence="11">
        <location>
            <begin position="1122"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1201"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1202"/>
            <end position="1214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1336" mass="152333" checksum="F25DAAEDC8767789" modified="2017-01-18" version="1">MKNLKVLNKSRIVADSQTCPDLPILDSCFDLISDSLTLALGSEEFAVIEIQQFQKNGAVRVLASVPFREGDTLLSLSHFEDSNQLVLIFSKGDIITATYDPSSLDIDQTFVEIIGSIDCGIKSAKWSHDEEILSIVTNEHNLLLFSRFLDLVLEKKIDPSDLQLNNQVSLGWGKEETQFKGRGIKQIERERKILKNAGLNIDSENAILCDPTIKEIQAGNLSKFDSQSYEISWRGDCQFFAISCIETLPEKDQTRRVIRVYSREGDLISCSEPIDGQEHSLSWKPQGSLIASTQRRFEPEINDLVLDVIFFEKNGLRHGEFDSRLNASLDNIYQIDWSSNSEYLLLQLESSVQLWYVKNYHWYLKQEISAYNNEKILFTKFHPEKPSKLMIGTSASIEIVDMSYTTTKGCTSPPHDAGVNLVIDGMTCMLTPFAKASVPPPIAYRDIDVDERSINSVSISHNNKLISILTSSFLYLFHGDENWDFKLTDRFRITDISQNSIYPRQVTIAGNDKVYVLFDEVLMNLSSIAEFEAGGDSLTLKRVTPVPSTKVITLSTDLEFESLVFETIDGSIFSLDANSEILSLGQFPQICTDFIVADLTKTEDGSENDTFLSTIKVPIGLMSSGKLYVNSKLISQGVTSMLVTGSYLLYTTAQHQLKFIHLLQNETLYNDEEFKDTVASETDVNFGLHDERIRNIERGSVLVNAIPSKSCVILQAPRGNLESIYPRIMVLSDVRKAIKAADYKTAFLTCRVHRIALDILHDYDPELFFKNVEKFINQLGKVEYLDLFLSCLLEQDVCETKYKESTGSNTTTAQEITDNLSKINLNRTEKGLEKTRKICDAILEVLLNNKKYKQQYLQSIITAYAAQKPPRTQEALELISTFDNDQEIEKSVQHLCFLLDVNKLYNIALGIYNIPLALVVAQQSQKDPKEYLPFLQGLYEETELRRRFMVDSYLKKFDKALNSLIQIPISEKHDIKEEIIEFIIEHELYKHALSLYKHDEENFNVILRHYASFLQATQKYTQAAIIYEKLSKFEEALDNYIWGKKWREAISIALKPEYKESKLIATCNSLIESLTLTHEYKAAAYISFKYLNNIKEALEYYGKEYEYSTAIQLCLEEGKPELIEEVVDPSIREGFGSIAELLADCKGQIESQLKRLRDLREKKNLDPYAFYGEIRGNENTPDNVSIAPSETSTKESFFTRYTGKTSGTAKTGASRRTAKNKRREDRKRARGKKGTIYEEEYLISSIGRLIDRLEITKPDSIKLLEAMVKRNMVSQAYLIQSNFVDILQLLKDTVVEIYTVDKRDRERLDDNGMIYYVDEIPVPVIKDFPALDVLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND76</accession>
    <name>A0A1E3ND76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_18548</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015180.1">
        <property type="nucleotide sequence ID" value="XM_019162054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND76"/>
    <dbReference type="STRING" id="763406.A0A1E3ND76"/>
    <dbReference type="GeneID" id="30178741"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000016">
        <property type="entry name" value="probable D-lactate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="41184" checksum="B12C7F7276A446B9" modified="2017-01-18" version="1">MDKIVELHADDLDVVVQPAVGWEDLRTYLADYNLMFGPDPGPGACIGGMVATSCSGTNANRYGTMRENVVSVTVVLADGTVVKTKRRPRKSSAGYNLTNLFVGSEGTLGIVTEVTLKLHVKPRFENVALISFPSLTGAATSVADFVQEGLQLNAVELLDEKMMHFVNYSGQSETRYEEAPTLLLKIGGSSQETTDSVTKTVRAIVKRNGASSFKFAASEEEKFQLWNARKVCLWSSIQYGKEQVDPNILVWTTDVAVPISRFVESLEATKAEMEKSGLVASIVGHAGDGNYHTVILFTEEQRQIAAKLVEEMVDRALAVDGTVSGEHGIGVGKKEFLVNEVGEDTIALMRKIKFAVDPHKILNPDKVFAIDPVNDRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDB0</accession>
    <name>A0A1E3NDB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone acetyl transferase HAT1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_20556</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015220.1">
        <property type="nucleotide sequence ID" value="XM_019162161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDB0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDB0"/>
    <dbReference type="GeneID" id="30178848"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="3">
        <location>
            <begin position="5"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="573"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="720" mass="79734" checksum="84E110A6D35EE3DA" modified="2017-01-18" version="1" fragment="single">DPDKWTCSANEALHVSLAEPSQAATFPPAYTYPLYGDAEQIFGYQGLRLDLAFDCLSMKPLLTCTYAARLADASGPAITSADAAAPLRAVLPPGDYVSGSEPAWRDAVAADAFALPQQALVRTYRTRAGDTCDVYGFRVGGDPPSAADALGKKLLRRVQVLALLYIEAASYIDLADPAWSFYAVYKRAPKPVFVGFCTCYQYWRFSTAPAHDSAPPFPDASFRARLSQVVVLPPFQAAGHGRELYRTLAGPHGVWTRDPRCVQITVEDPTEQFDQLRDRCDLETALALNAFRNIHRIPLDSTSHRRFREQLKLTPRQADRVFEMGLLWLLAHKEGAWIAGLDAGEPAVRRLVKRRVYLANREGLAALGDASLVRSKLQEVYERVAQDHKKAAADVSRPLKRAAEAGSMHRTYSMRSARAPTAAQLATPPPPPSSTKGKFFGSTSIADSFRKQAAGSFGPEMSRKLSQLIKIEKSLERSLEGVSNERKMVAKQLSIWGADNDDDVSDVTDKIGVLLYEIGELEDQFIDKYDLYRITLKSVRNIEGSVQPSRDRKIKITDEIAHLKYKDPQSPKIAVLEQELVRAEAESLVAEAQLSNITREKLKAAFNYQFDSLRELSEKMALIAGYGKALLELLDDSPVTPGETRPAYDGYEASKQIIIDCENALSAWTLDNSYLKEPLLSIHQGIEDVYTVPEGIVEGDAEVVEADDAEHEWAHHDDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDJ2</accession>
    <name>A0A1E3NDJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin MVP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sorting nexin mvp1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_19393</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015303.1">
        <property type="nucleotide sequence ID" value="XM_019162117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDJ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDJ2"/>
    <dbReference type="GeneID" id="30178804"/>
    <dbReference type="OrthoDB" id="10064318at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000042">
        <property type="entry name" value="Sorting nexin mvp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028662">
        <property type="entry name" value="SNX8/Mvp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045734">
        <property type="entry name" value="Snx8_BAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554:SF1">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19566">
        <property type="entry name" value="Snx8_BAR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="20"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="489" mass="57967" checksum="6AE2A4D37F55DA95" modified="2017-01-18" version="1" fragment="single">VWSDDEIKKFNPLTLKNATDGLIVRVREIPEKEGLVFKHTNYLISHTLKFSSEYLVSSDKTKSSRDSRAKGQNEDGTKVIRRYSDFAWLVEVLWKKYPFRLIPELPPKKFALASSNDVLFLQKRRRGLQRFLYQLCKHPILSKENLVIMFLTVPNDFSNWKKFANIELNDEFDGIRINLPRRFKIDLSDVLKGLRDNESDFEDNEDITDESEENHTSSNRNEFVINNINQIWNESPVNFLDKDFITNLNSLYESLSRLAEFWSKLYLLVERIEKRELALSADHQRFAIFLENMIKTDADVFSMDNLIENKSKRIDDETQNMTIINSILSQVCKYFVGSRKLKEEEMLVINNEILESWKSFQDYLIGLHFLIERFFNYKTDSEKEIQNLLQRIIKTNEKIKQLKMKSDIRGSEIDKHINVLITSMDQLSMLISRIILVKTTFVNEFKLFQKVKYLISEVFQNWFDERSKFQETKNDNIQRLLNDLKDLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDN9</accession>
    <name>A0A1E3NDN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-mannose transporter</fullName>
            <shortName evidence="5">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_74822</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015329.1">
        <property type="nucleotide sequence ID" value="XM_019164882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NDN9"/>
    <dbReference type="GeneID" id="30181569"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="345" mass="37894" checksum="FC57AC9AE4A23A75" modified="2017-01-18" version="1">MSQEKQDVSINSSPVAAAAALGQQPSQLARVVNSGPVSILCYCASSILMTTTNKYVVSGHHFNLNFFLLAIQSIVCLIAIATLKFFGVITYRQFNANEAKKWSPIAFLLVLMIYTSSKALKYLAIPIYTIFKNLTIILIAYGEVLWFGGEVTPMALGSFVLMVLSSIIATYGDTDASGSMNFNVGYFWMALNCFSSASFVLFMRKRIKLTNFKDFDTMYYNNLLSIPILLVSSLLLEDWSSANLDLNFPPASRVSVWLAMIISGATSVGISYCSGWCIRVTSSTTYSMVGALNKLPIALSGLIFFDAPINFFSISSIFVGFSAGLVYVVAKQKQKEMQAKQELPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDS5</accession>
    <name>A0A1E3NDS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74876</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015385.1">
        <property type="nucleotide sequence ID" value="XM_019164899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDS5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDS5"/>
    <dbReference type="GeneID" id="30181586"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="14"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="375" mass="40937" checksum="9069C486A126B95E" modified="2017-01-18" version="1">MSKVNYPEEFAGFAIVDTKEWNKPKYTTYKPKEFGDYDIDIEIECCGLCGSDIMTAKGGDHGWGSITLPQVVGHEIIGKVIKAGPKVSLHKLGDRVGLGAQAFACLDCNRCKHGNEQYCPHTVTTYDGVYPDGYVSQGGYASHVRAHEYMCFPIPEKLDSIHAASLCCAGLTVFSPLKRYIPKNLPEGQVPKVAIVGLGGLGHLAVQFCKALGAEPYVFSRTDNKKEQSFELGATGFVTTGTEGWEKPFFDQFDLILNCAIGLSGLNIDAFLSILKVEGRFCSVGLPAVDEKYNVSPFTFFGNGSCLCSSALGSREEAVELFQMAAENNIVPWVETVPISEENCQTALTRAWDGDVRYRFVFTEFDKAFGTNGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEC8</accession>
    <name>A0A1E3NEC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_18367</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015582.1">
        <property type="nucleotide sequence ID" value="XM_019161969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEC8"/>
    <dbReference type="STRING" id="763406.A0A1E3NEC8"/>
    <dbReference type="GeneID" id="30178656"/>
    <dbReference type="OrthoDB" id="10258141at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170071">
        <property type="term" value="C:CROP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140312">
        <property type="term" value="F:cargo adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900102">
        <property type="term" value="P:negative regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="684"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="684"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="823" mass="96864" checksum="0D80C5EDE7892873" modified="2017-01-18" version="1">MTLEEQQRLDNSITIIKNEIYQMRKCLESKHKFMDSLKHASNFLNELRTSSLNPKQYYELYILIYDGLNYLSNYLKDNNKESNNNYLIDLYELVQYAGNIIPRLYLMITVGSVYMGLEDAPINEILNDMLEMCKGVQNPIRGLFLRYYLIQKTKSFLINFENSSNLDKVINFIVANFIEMNKLWVRLQHQGHSSERARRVEERNELKILVGSNLVMLSQLEMVSKELYNEKILPEILQQIIRCKDTIAQNYLLDVIIQIFPDNFQVYSLQTFCKALLLVNEDVSITDLLNSLIERLIDYRKRNSTESLGDFSFLKTFVEFIENLNQNDPEFNIKNYCTILNGILKLSLIYSKEDDEVLDNLNLIYKLSIDKFNLIKDSSEEIDDFRIILKSIENFEDFNNFFKINDDNYFKLFNLQSLSIKKEIGTLILNNLIDNDIRIDNLESLQMVFKYLNESILVKEDKHQVSETKRDLFGDSSTDNSASNELIELDIYHEFLNKFLHLVYNKDYKVQYALLNECEVHLSKGNYYRTYPTLISILIRLIRKRNFEKADKKELVNMFNRISKLINMIKDKMPIKALNLNLNCAQISNECLLNNISYEFFIESFIIYEEMIIESKIQIQCLMNIINKLISLDQMIILDVEDFNKLITKATLYGSRLLKKTDQCRAIYNSSHLWWINKEEDAIAEGDDDEEKEDSAEPEETGESHLQENANQISLKKDDKRVLECLQKSLRIADSIMDNNVSVELFIEILNQSIYYFIHGNEMINVRYLNGLIELIGNNFKEIGQTNGQIEGPYLSTWKHYQRTLKYIKEQRVVDARFHEIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEV5</accession>
    <name>A0A1E3NEV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18079</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015790.1">
        <property type="nucleotide sequence ID" value="XM_019161871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEV5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEV5"/>
    <dbReference type="GeneID" id="30178558"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000028">
        <property type="entry name" value="DnaJ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052814">
        <property type="entry name" value="Peroxisomal_DnaJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006:SF2">
        <property type="entry name" value="PROTEIN CAJ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="471"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="485"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="534" mass="59607" checksum="D0CF5342F4ED85DD" modified="2017-01-18" version="1">MVKDTEYYDLLGVSPEASELEIKKGYRKAALKWHPDKNPDNPEAEKKFQEIAEAYQVLSDPQKRTIYDEVGKEELNQQGNGASQDIDPKEFFSMIFGGEASKDYIGELNFIQMMFEAEEQADEQEKEGNEGAGNGDVKAESGAGDKSSSTHVTLHDGSKKTFSDLNEESKTATKEKRKQGIDAETVRKQREEEEKKVKELAEKLKAKMDPLLESVKGGHLDKEGKDWQIFEKKISDDIEDLKLESFGLDICHLIGKIYLFKATSYLKSKKQFTGGFHKISSNFKQSKDTVRGMMDMLSSATEAQNTLEAMQQLEGVSLEDMDPYKRAQYEQAVTGKFISVAWASSKFEIQQTLYGVCNMLLNDKEISSDKRKLRAEILIEMGRMFAIAKRDESEEEDQQVFERLMKEANEMKAHDLKREAHMKARNEMRSGLAEEKAKRRLNHDSHDANQSSSGAANSTTSGAYSYTYGKGDGKPVDTKQEDKPSTTAAATSSSSKTATAPAATVSTAASPRANSDGAKFGFGFGRKIKKVFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF68</accession>
    <name>A0A1E3NF68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Imidazole glycerol phosphate synthase hisHF</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Glutaminase</fullName>
                <ecNumber evidence="12">3.5.1.2</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">Cyclase</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_60380</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutaminase domain produces the ammonia necessary for the cyclase domain to produce IGP and AICAR from PRFAR. The ammonia is channeled to the active site of the cyclase domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">In the C-terminal section; belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="12"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015849.1">
        <property type="nucleotide sequence ID" value="XM_019163431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF68"/>
    <dbReference type="STRING" id="763406.A0A1E3NF68"/>
    <dbReference type="GeneID" id="30180118"/>
    <dbReference type="OrthoDB" id="10254903at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000094">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000039">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014640">
        <property type="entry name" value="IGPS_HisHF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036936">
        <property type="entry name" value="IGPS_HisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 12 15" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="16">
        <location>
            <begin position="7"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="369"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="407"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="479"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="505"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="528"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="14">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ44736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="561" mass="61343" checksum="E9E344701B8FB975" modified="2017-01-18" version="1">MSSVVHVIDVNSGNLQSLSNAIAFLGFQVRFITNGNDPALQTCEKLILPGVGNFGHFVKELHAREFIEPLRKYINSGRPIMGICVGLQALFEGSEESQGIRGLSILPGDLKKFNTSKKSVPQIGWNSISLRLPSSSDANSLYGISEDKFYYFVHSYAAIRTQEQIDSLANEGWSLALCTYFDETFVAAVSKDNIFATQFHPEKSGLAGLKVIQSFLNGNRYFTVKLEKPDLSKIEKTETGLTRRIVACLDVRTNDQGDLVVTKGDQYDVREKSDAGNVRNLGKPVEMAERYYSQGADEVTFLNITSFRNSPIKDLPMLEVLRLAAKTCFVPLTVGGGIRDVVDPDGTEHSALEVASMYFHAGADKVSIGSDAVRAAEEYYANNNQGLGTSPIETISEAYGAQAVVISVDPKRKYISDPKECKYKTIKTSVPGPSGEQYCYYQVTSQGGRKVHELGAVELAFACEKLGAGEVLLNCIDKDGSNSGYDFELINMVKNAVTIPVIASSGAGNPQHFVDVFQNTKTDAALGAGMFHRGEYKVSDVKDFMKKDGLLVRIDDTQLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFC0</accession>
    <name>A0A1E3NFC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED8</name>
        <name evidence="11" type="ORF">PICMEDRAFT_13478</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015932.1">
        <property type="nucleotide sequence ID" value="XM_019160065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFC0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFC0"/>
    <dbReference type="GeneID" id="30176752"/>
    <dbReference type="OrthoDB" id="5329317at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019364">
        <property type="entry name" value="Mediatior_Med8_fun/met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074:SF9">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10232">
        <property type="entry name" value="Med8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="271" mass="29987" checksum="5AC179907292ED05" modified="2017-01-18" version="1">MSVPPAPVPATQQAFPPLNFEDTPVEDLEQLRPRLTQLTHSLRKLEEALRVLHYTQSNAELTAIQNQFNVILQQLASLSRTLQAHKEILQVTNVFPNREFNTMSGLLFNLLRKKLTPEVEEWIKNATQIATAHPPHSAHSIPPSTATSTVTQNTINTAAATTNSHLKSSADADALETLLFRDDQLTDEYVEYAHTTLEDYMFGGYLTKSEIDSGLKISDVLKNVKKEDLESPMSSPSPIAASSPSPSGPPVAERDLLRFIYQGLDSNVSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFC5</accession>
    <name>A0A1E3NFC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">homocitrate synthase</fullName>
            <ecNumber evidence="6">2.3.3.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_74517</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="6"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015937.1">
        <property type="nucleotide sequence ID" value="XM_019164790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFC5"/>
    <dbReference type="STRING" id="763406.A0A1E3NFC5"/>
    <dbReference type="GeneID" id="30181477"/>
    <dbReference type="OrthoDB" id="2015253at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00028"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07948">
        <property type="entry name" value="DRE_TIM_HCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000002">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000032">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02222">
        <property type="entry name" value="Homocitr_synth_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048253">
        <property type="entry name" value="DRE_TIM_HCS_fun_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011872">
        <property type="entry name" value="Homocitrate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02146">
        <property type="entry name" value="LysS_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF48">
        <property type="entry name" value="HOMOCITRATE SYNTHASE, CYTOSOLIC ISOZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="16">
        <location>
            <begin position="21"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ44824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="415" mass="45951" checksum="D93DF6C5E3D26777" modified="2017-01-18" version="1">MSATNPYAPNPSDFISNVPRFEVIDSTLREGEQFANAFFTLENKIKIAKALDDFGVDYIELTSPVASDQAREEVEAICKLGLKSKILTHIRCHMHDARVAVETGVDGVNVFIGTSNFLRQHSHGKDMSYITKAAIEVIEFVKSKGLEVRFSTEDSFRSDIVDLLNIYTTVDKIGVNRIGIADTVGGANPRQVYELIRTIKSSVSCEIETHFHNDTGCAIANAYTALEAGAKYIDTCVLGIGERNGIVPIGGFMARMVVSAPEYVKSKYKLNKIRDLENLVAEIVQVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILNPTDFGLTRYIHFANRLTGWNAIKARVEQLNLNLTDDQIKVVTNQIKQMGDVRPLGIEDVDSIIKAFHAKEKDNTSNANENENEQPGAKRQRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFM5</accession>
    <name>A0A1E3NFM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 18">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_78689</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. Negatively regulates the biosynthesis of acetyl-CoA from pyruvate in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 18"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016037.1">
        <property type="nucleotide sequence ID" value="XM_019164964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFM5"/>
    <dbReference type="STRING" id="763406.A0A1E3NFM5"/>
    <dbReference type="GeneID" id="30181651"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000003">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000019">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="371"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ44924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="416" mass="44429" checksum="0E8DB2CB103DAB70" modified="2017-01-18" version="1">MSLANKLSVKDLSIAGKRVFIRVDFNVPLDGDKITNNQRIVAALPTIEYVLEHKPKYVVLASHLGRPNGEAVAKLSLKPVAAELETLLKKKVIFLNDCVGPEVEKACAAAPEGSVILLENLRFHIEEEGSKKTSDGKVKADKAAVEKFRQQLTALADVYVNDAFGTAHRAHSSMVGFELDQRAAGFLMAKELEYFSKALENPVRPFLAILGGAKVSDKIQLIDNLLDKVDMLIIGGGMAFTFNKVINGTSIGQSLFDKDGAEIVPKLMEKAKKNNVKVFLPVDFVTGDKFAADATVGHATVDSGIPDNLMGLDVGEKTIKEFEGAISQAKTIVWNGPPGVFEFENFAKGTKSMLNACVQSCANGNIVIIGGGDTATVAKKFGAADKLSHVSTGGGASLELLEGKDLPGVAFLSDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFV0</accession>
    <name>A0A1E3NFV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pescadillo homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nucleolar protein 7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NOP7</name>
        <name evidence="8" type="ORF">PICMEDRAFT_17494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the pescadillo family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016105.1">
        <property type="nucleotide sequence ID" value="XM_019161623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFV0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFV0"/>
    <dbReference type="GeneID" id="30178310"/>
    <dbReference type="OrthoDB" id="10264910at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17709">
        <property type="entry name" value="BRCT_pescadillo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000067">
        <property type="entry name" value="Pescadillo homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03028">
        <property type="entry name" value="Pescadillo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="7">
        <location>
            <begin position="356"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="303"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="454"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="471"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="604" mass="68795" checksum="986FEFAE0F515586" modified="2017-01-18" version="1">MGPIKRKGESGNAKNFITRTRAIRKLQVSLADFRRLCIFKGIYPREPRNKKKANKGSTAPVTFYYAKDIQYLMHEPILQKFREHKTFAKKLTKALGKGEIGDAKRLEENRPKYKLDRVIKERYPSFLDALRDIDDALSMMFLFTNMPATSAVGAKITSQANKLTNQWLAYVARERALKKVFVSIKGVYYSAVVKGQEVRWLVPFKFAQNIPSDIDFKIMHTFLEFYSTLLHFVLYKLYTDSELTYPPKINENRLKGIGGLSVFILETNEQRSLALPSEVTGESSKSAKETKISGEEMSKAFAADKKEAALEQEEEEEEKEEDERIENVENTDLDEFKDNSKNTGDILAQPSKYANQVSELFSKFVFFIGREVPVDVLEFVILSAGGKVVSEAAIDELENSKAVDLSSVTHQIVDRPKILKKVQGRTYIQPQWVFDCVNKSALLNVSDYAPGETLPPHLSPWGDAGTYDPEAPLEEGEEEGEEEEDAEDAEDAEDAVDAVDAEDAEEDEELREQKELELEMAGVKSSVVSKDQKSGNKRKHTEVAGKEDEDEKNLKMIMMSNKQRKLYKKMQYGLNKQDVRKLELEKKRKLLSKKKQELSKLTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG47</accession>
    <name>A0A1E3NG47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016195.1">
        <property type="nucleotide sequence ID" value="XM_019161655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG47"/>
    <dbReference type="GeneID" id="30178342"/>
    <dbReference type="OrthoDB" id="3988607at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="187" mass="21432" checksum="86B99FBAB153AB37" modified="2017-01-18" version="1">MSTTLVRAKELPRPTQQYMSDMGEQPTPLTESDIAAKEVSDIELPQATLDEKAEIKKAMKKRSLRMTFWQLFLITLLGSSTLNIMREKNLRDELDENFEKKFGVLKRLQEEAEQGKITVEGVRETLKSWNERFVDVFDLSPIEVKGVEGLDEQKLKLAFNKVKGIEIDESNGEHSPAKTTEKLDKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGD8</accession>
    <name>A0A1E3NGD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thiamine pyrophosphokinase</fullName>
            <ecNumber evidence="7">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74654</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>thiamine + ATP = thiamine diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11576"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the thiamine pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.2" evidence="7"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015526.1">
        <property type="nucleotide sequence ID" value="XM_019164834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGD8"/>
    <dbReference type="STRING" id="763406.A0A1E3NGD8"/>
    <dbReference type="GeneID" id="30181521"/>
    <dbReference type="OrthoDB" id="25149at2759"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00597"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.320">
        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016966">
        <property type="entry name" value="Thiamin_pyrophosphokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031057">
        <property type="entry name" value="Thiamin_pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="8">
        <location>
            <begin position="259"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="329" mass="37294" checksum="EF02CA05572D5D33" modified="2017-01-18" version="1">MPTGEKLEVVEKVVENPDSISIKHTIDTNVELDTHVISPLKYFEARSRFNDESDRHTTVLLILNQQINILPDLFAKLWNNATLKVCADGGLNRLRDYDSTSSAYIPDYVVGDLDSAKKENIEHYQSLGTKKILQSSQYYTDFMKALSLINTFFNFPDIISDINHLDTVDALEKLEEERSAESGTKVKALKIVVLGGVGGRFDQTMATINQIWNLSSRRPHLQFVILNPEHTEIIVLLKPGFNFVAYPRFTPQEETEVFGIITEKSRPGLRNVGVLPILSDAVITTYGLKWDVENWRTGLTTKMSSSNLQVGKEGFIIWTNKHLFVDFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGH5</accession>
    <name>A0A1E3NGH5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA-processing protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73964</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016299.1">
        <property type="nucleotide sequence ID" value="XM_019164632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGH5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGH5"/>
    <dbReference type="GeneID" id="30181319"/>
    <dbReference type="OrthoDB" id="272245at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="7">
        <location>
            <begin position="30"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="294" mass="32761" checksum="D35F53C67E8BA724" modified="2017-01-18" version="1">MVLSVAESAFIYDSLVASHPIRPDSRKPTQYRPLKANCGFLPNSNGSSRIYNSDGTECISSVKAKVIRSSEISDLINVEINIFGEKDNTTLCQHLTTLIKDSLIDSFDFSVLKLTDKYYFQLYIDISILYLPEDFRYSSYTLYSILSLISMGVYIALKSAKLPLLISDKKDFEVEEEPTFSDDWEVSAFLIPRNAKRSFQPALIFIVGLAGNTVIIDPSLEEEQVLEHGICVTWSNSKVSAPVQSLALSTTNNKGLKPQMILKAMDLVKIVASEVGDALDLIAEQDIDEFDAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGI6</accession>
    <name>A0A1E3NGI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">26S proteasome regulatory subunit RPN2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_74031</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the proteasome subunit S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016373.1">
        <property type="nucleotide sequence ID" value="XM_019164655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGI6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGI6"/>
    <dbReference type="GeneID" id="30181342"/>
    <dbReference type="OrthoDB" id="261572at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000017">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016642">
        <property type="entry name" value="26S_Psome_Rpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040623">
        <property type="entry name" value="RPN2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18004">
        <property type="entry name" value="RPN2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome regulatory subunit RPN2 C-terminal" evidence="8">
        <location>
            <begin position="760"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="810"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="818"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="834"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="951" mass="105238" checksum="F9C2B0405AD92387" modified="2017-01-18" version="1">MSVITSAAPYLSLLDEHDPSLKVYALQSLDKQVDQLWSEIANRIADIEQLFEDSHFSHRELAALLASKVYYNLGDYDSAVKYSLLAGKAFNTNESSEYVETIVSKCIESYISLSQSKYADDSVVIDRQLTSIFEQMLTKCVNDNEIKLALGISLESYRLDIVKNIIDTQIKNKVSTDNIMGLIGYVLTCSTTVINDVNFKTETLNGLITILLSFDNPDYFLVTKIIVQLSDSVFAHRVLKKLLSSDDNVSMAYQIAFDLVGIASQELLTSTSKFIEADSEIDQTNSKTIRLKRILSGVPTCDLDITFLSEHRNVDISILNASKKALDGRNSLYHSAITFANAFIHSGTTDDSFFRSNLEWLGKANHWSKFSATAAFGVIHKGNLSQGRAVLEPYLPGSTSNAHTNGGSLFGLGLVFAGQHGQILDYLRDTIELNSNSVENKDNDIILHGACLGIGVSAMGLADLDLYNELNVVLYSDSAISGQAAALAMGLVMLGTGNEEALSDMFTYAQETQHETIIRSLAVGMALISYGKENKAEPYIKKMLDHKNSILRYGACFTIGLAYCGTSNRSAIKKLLHVAVSDSSDSVRRVAVMCLGFVLLREYTKVPTIVELLAQSHNPYVRYGSAMALGISCSGTGLKSALDVLDPLSTDAVDFVRQGATLAKSMILIQQNEKTHPKVKDFREKLASIVSNKHEESLARFGATLAQGILDAGGRNTTIQLENTQTNTLNMKAIVGLTIFTQYWYWHPLSHFLSLSFTPTSVVGITENLKIPKMKINCHCKEDVFGYPPKVEVQVAKQAEKVATAVLSTTHRARNRAAKKEKSKEDKMDVDEMEDRKDEDKSPEAHTGVENEELQDNQFQSLYAKEPYEIDNMTRVLPAQMKYVTFAKNSRFTPLKKFRSICGVVVLKDNEPTKPFDKIKTLREMNISEAPVPLPFTISPELDNFEGLESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGJ0</accession>
    <name>A0A1E3NGJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_18088</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015801.1">
        <property type="nucleotide sequence ID" value="XM_019161875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGJ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGJ0"/>
    <dbReference type="GeneID" id="30178562"/>
    <dbReference type="OrthoDB" id="409625at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3 5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="6">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="89" mass="10065" checksum="4074853ABE4F943F" modified="2017-01-18" version="1">MSFQPINPKPYLSSLIGLKVVVRLKFSGTEYHGTLATVDNYMNLLLDKDVIEVEQKLGEEPDLSKGTPLESELFVRCNNVLWIGKEPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGX6</accession>
    <name>A0A1E3NGX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitobiosyldiphosphodolichol beta-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.142</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Asparagine-linked glycosylation protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_22704</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.142" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016487.1">
        <property type="nucleotide sequence ID" value="XM_019162274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGX6"/>
    <dbReference type="GeneID" id="30178961"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004578">
        <property type="term" value="F:chitobiosyldiphosphodolichol beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="410" mass="47111" checksum="0B27D2AF6632E601" modified="2017-01-18" version="1" fragment="single">VVIVVLGDLGHSPRMAYHARSFQKLGYQVNLCGYLESDLPEFLSSPDISIYDIPVIKNTKKLPYLMFAICKVISQIVELTTLLKDVLDDSTRFVLIQNPPSLPVLFILGVLKRYWVPNVKIVIDWHNLNWSILNMKYQNENHPVVRLMKLYEKLCSTYFADLNLTVTAALKKYLIKQFELNEDKIVTLYDRPSSIFEPLNSQQSLQKIVNKNLALFSELGYNQVTDRILITSTSFTPDEDFTVLVDALVELEKTMLLQKSKLKIIMIVTGKGPMQPQFLHSIKSHNWEHIKIRSVWLPITEYPNILKIADLGISLHYSSSGLDLPMKIVDLFGSGVPVLSMNYPAISELVKDGVNGMTMQRNKDGKEMADKIYNIFFNDKPSYIKIKEGALLESQRRWDNEWNEKLSKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH36</accession>
    <name>A0A1E3NH36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UNC-45/Cro1/She4 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015991.1">
        <property type="nucleotide sequence ID" value="XM_019161590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH36"/>
    <dbReference type="STRING" id="763406.A0A1E3NH36"/>
    <dbReference type="GeneID" id="30178277"/>
    <dbReference type="OrthoDB" id="5574718at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024660">
        <property type="entry name" value="UCS_central_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11701">
        <property type="entry name" value="UNC45-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UNC-45/Cro1/She4 central" evidence="1">
        <location>
            <begin position="86"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="724" mass="82079" checksum="AF3D98CF30036571" modified="2017-01-18" version="1">MSEPITAAELCLNLKKDLHEISLLSYDARKKHLESTSKSLLRSSFQLLSHEKNNLPDSQVSQLSEYLIAYLGFDHFISVEALMSLYRLKSLNDDFDTLLEEKIDDLIETQNSKSDVYKVFKIISQLFAFDPVLSSAIFTKKAPFINLLTNEVTLLTNNLGTLNPESIKNLNIILLLLSNACMEETSRSMIATMHINILLKCLSITDDAVSQSRCYAATTTVKLWRLINPEVLENNAVLLSLNNLSDIIIDCLHQGLESSVEGLSLLCTNIQIKKKVRNEKVMETLFRLMKSKDHTVYGIISILALVTLPIRVLKVQQSSVMSLKDSNSISNIDAMTNKKVNATKLEDDIVSIQYANQNIIKRNFFSIQVVPIFKSLESSKGLVGQCLKLMHNVAFPDIDQTGKENNFLKDKANYATYISEIREIIKLLTAFLIGTSQNIKYKHDKFITYDTEKTEMPEHDLELRSIAIKSLCSPEISARVNEVYGTDNEEFALSPVPFILEILVQHDIDTGCSLETKQTPFSSIKKQIFSTFDVYYAFVALAAIASLSFEKVKQSIFTLGFDCIINALNSGDEKLQFASLQLLNEISDIPLCIANLFNWATETDEHYQNFSILCHLLQSNNYDSQCLVLQIFYSVSRFEIVTEKLCESELFCSNLNKIFQNQDSDDALIYYALLLFGQLIPLRGKCAGYDYLKLFDESKNIFQKHTQSRNEQIRESASLIVKHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH62</accession>
    <name>A0A1E3NH62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_74253</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by a SNO isoform. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016608.1">
        <property type="nucleotide sequence ID" value="XM_019164722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH62"/>
    <dbReference type="STRING" id="763406.A0A1E3NH62"/>
    <dbReference type="GeneID" id="30181409"/>
    <dbReference type="OrthoDB" id="1660966at2759"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="11">
        <location>
            <begin position="6"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="9">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR029271-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="300" mass="32685" checksum="3F8B1F4B0189A9FD" modified="2017-01-18" version="1">MTLSKFQIKAGLAQMLKNGVIMDVTTPEEAIIAERAGAVAVMALERVPADIRKENGVARMSDPQMIKKIMDSVSIPVMAKVRIGHFVEAELLEELAVDYIDESEVLSVADRFDHINKTDFKVPFVCGSKNLGETMRRIEEGAAMIRTKGEAGTGDISEAIYHIKLINKELEDMMLKFGGLKKVTGELVEFAKLHRVKPETVMAIIENNGKLPVVNFAAGGIATPADAAICMKLGCEGVFVGSGIFKSENPELVARAIVNAVNHYDDSKELLKSSTGLGKCMFGVNISTLRQEEKLSSRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH78</accession>
    <name>A0A1E3NH78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016613.1">
        <property type="nucleotide sequence ID" value="XM_019164363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH78"/>
    <dbReference type="STRING" id="763406.A0A1E3NH78"/>
    <dbReference type="GeneID" id="30181050"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="436"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="845" mass="97344" checksum="2690C0ABBFC7A773" modified="2017-01-18" version="1">MYGEQYSSSNILRSLPFRSPLALLLTPPEILDQSLMHKSNAAESVINSLSQKLFEQKEVENSSWKLTTGFHNNRLTEGSDEISVYTKKRQTEHIYDPRITMAVYLHHLQEQHSSGQSSIPFSWEDWVDLSYLNRFLNGKSRSCASFLTDYEISLKYEMNDSLPSTEYANRSFCLDNIDYLKTHNGKYKDEKLLPGFNFMQRINEKSNFIGKIFNAKSFILSYAPTPSLIYFLNENGTYYKTKPYQATSMMQNGLFESFTDKSTFNGFNPVEELGKLGDKYLTHNENDFAIQLLKTKNFQLNIPEGNFYLDTDEHFADLYNKDFSLLEDNEKRFLESVNYSKAADAEDVKKYFKEVNILWPASYNAHKLKENGGHYDARFFSGFVTEMPLSDFTFHSPYYTPEELDRKPLDNPTSRRTIILSHLLHTLLTATFHDGLYMFPAHGSLLSWYFTSMSFPYDGDGDVQMPVTDLAEFCLRYNNSLIVQNPKYGMGKYYIDCTSSLTHRGKETGTNNIDARVIDVDTGMFVDVTGIGVSADRLTAPGMNKLDGWIPKQVKSKYPLSKVDSRRGRRQGALASNKFKHEGVKSIQKAEAEDKETVNKVLKIHSENRIYNCRNDHYYTYEQLSPVRLSLFEGAPTFVPASKKSLTSILEQEYSPLSLRRVAWDDWVFLKPLKMWVHADDVHWACVVNNLQPTTAVGKSKNKRKAKGNKKNVKGVNVVGCYIGNEATIISEMIKSSLYEITSEEVLKHRDERRPSMNIVEEFYHDNLYNSVHSTEMAIYYSDWEWKSPSIHDYHGMPTKWKDLAQWMLRDHPPPRIPMLDYLSYVEVADQGKEVVVSTSNYTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH83</accession>
    <name>A0A1E3NH83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin-95</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Karyopherin-95</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73722</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family. Importin beta-1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016031.1">
        <property type="nucleotide sequence ID" value="XM_019164564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH83"/>
    <dbReference type="STRING" id="763406.A0A1E3NH83"/>
    <dbReference type="GeneID" id="30181251"/>
    <dbReference type="OrthoDB" id="10263328at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042564">
        <property type="term" value="C:NLS-dependent protein nuclear import complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061676">
        <property type="term" value="F:importin-alpha family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046822">
        <property type="term" value="P:regulation of nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060188">
        <property type="term" value="P:regulation of protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000027">
        <property type="entry name" value="Importin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="410"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="870" mass="96264" checksum="5FB5D10293C90DDC" modified="2017-01-18" version="1">MDISVLLENAILNPNPADRQKAELDLSKLAEENWIQYVGLSIQVLGDESKKTEIRMLAGLNVKNQLTSKDSAKKQHQAERWIQIDQPTKTHFKQIALSTLLSSDDRVANQAASLVAAIADIELPRSEWPDLIDIIVENTKPEKPVHVKRASLLTIGYICESADPTNSVVAGYSNGILIAIVQGAQSSEESLIVRKTAINALVNSLEFIEANFQREGERNYIMQVVCEATTSSDFELQALAFGALAKIMSLYYDYMGVYMEKALYSLTVEGMKSKDDRVSCMAVEFWSTICEEELDIQINRETTENPQDDYVSFNFALIAISDVLPVLLSLLTRQNEDFEDDDWTVAMAAAACLQLFAQDTTNYVVQPTLQFVEANLGSSSWRNREAAVMAFGSILDGPDRNELKGLISQALPPIFSLIKDENLQVKDTVSWCLGRIAELVVDAIDTNTMLPEIMKALVEGLKDHPKVATNCCWTLINLTEQLCGDHASSDSTILSQYFPTVIPALLEVSSKSDNEHSSRTSAYEALSTFILFSAANDIPLVSAIATESINRLDSTIAMQTEYQTGRRALSAEDKALLEELQANILSLLTNIIRRIGHDIINVSDSLMTKFLSLLQIQTADSIIEEDIFMAISAIASSIGKSFETYMPSFLPFLTKALENVDSPVCNSAIGLVVDISHSLGDSFIPYCQGFMSILGNIISNVNIRRELKPLILSCFGDIASSIGNEFIQYLGVVMGICLSAQSIQPTDDTIETEEFILTLQESVLDTYVGVVAGLHDNPAPLSNYLPQLFNFLTMIYANPLLCNNDTVYRAAVGLLGDIAQIFQGTPLEYYKQEWITTFLKKAKNNPRYSQNTRDTAKWAREQQKAMAAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH88</accession>
    <name>A0A1E3NH88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17963</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016610.1">
        <property type="nucleotide sequence ID" value="XM_019161827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH88"/>
    <dbReference type="STRING" id="763406.A0A1E3NH88"/>
    <dbReference type="GeneID" id="30178514"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002109">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="148" mass="16884" checksum="B094142D0CE253EB" modified="2017-01-18" version="1" fragment="single">MQIFVKTLTGKTITLEVESSDTIDNVKQKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKVYTTPKKIKHKHKSVKLAVLHYYQVDGDGKVTKLRNECPKCGPGIFMANMENRQYCGKCHTTLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH89</accession>
    <name>A0A1E3NH89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016607.1">
        <property type="nucleotide sequence ID" value="XM_019160028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH89"/>
    <dbReference type="GeneID" id="30176715"/>
    <dbReference type="OrthoDB" id="3995094at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="43633" checksum="23ED0669967073CA" modified="2017-01-18" version="1">MPDILTQDGMLEFDNLLATNPLPDKLLLPDYKILDTSQKFSQPKENLLFSDLEKQMNLNAAQIHPTHFSIEDIQSHRTRREQHTQQVQQDYQTQKSIPDIHSKTNFNINSVISSRSYHQDLTHATGSYDSSPESNFPYTPVDSLESCTTDSNIDNELAKNIKAELFDDIHILDDRYNVQINTSNADNTASDPEILLNDDILDKEIEQIISAPLDVQIDSLYSTENERLAVNNQAQLNPHKRAYSVVRGTPTEDTLDRTKKRKVEETKTQSSQKETKFLKCFSILRANYLSVCNSYNEVVDKLSTVEAQNKILSQNLATKENEKEQWKIDKDRFLFEREDMKAMLDGLLHEVTVLRRRERQNKMRSTIGNMMLMKDDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHB0</accession>
    <name>A0A1E3NHB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 5">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="1 5">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74439</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="1 5"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015846.1">
        <property type="nucleotide sequence ID" value="XM_019164771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHB0"/>
    <dbReference type="STRING" id="763406.A0A1E3NHB0"/>
    <dbReference type="GeneID" id="30181458"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08598">
        <property type="entry name" value="PI-PLC1c_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="7">
        <location>
            <begin position="556"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="491"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="767"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="498"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="767"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="948" mass="108111" checksum="C67BD9C97A317898" modified="2017-01-18" version="1">MPPKEQLVPPPSAHEEAGDPSNYQPGSPVIEEDSVKLIDPFDPSDPDSFPPDLLSPGIDMIRVTRRKRIRRAFKLTLPSLTLTWNSKSKSKVEMDKIQSIRVGEDARNYREEFNVSKEFNALWITVIYISSNSSHSSALLCNDLKALHMIATTKRNYDIMLKTLKLLSQWSKEQESLVSCADVNEFSKIKWDNKTKKLERQLLSFEDVLKLTSELHVYMDVSYLRLYFDQCDTNKKKSLDFPQFQTFVSKLRSRPEFGAIFHGLNIRNGKMLLSDFRIFVRETQNESSLSDTLINDIFVKFSHGKSLDSYMSTSDLSYYLNSPYATALFNEESLDPHYYSHPLTDYFISSSHNTYLLGKQFHGFSSIEGYIHALQRGCRCIEIDVWDGISTSTKEQHPIVTHGHTLTNSIEFKLVVDIIRKYAFITTPYPLIISLEIRCSQESQLKCVAIMKTIFGDMLVLHSKVNESIMPSPEELKHKILVKVKKSKSTTIIEPQGSPHSLHSSFSTSSTQSDDINPNYTENKYDEEEVTHRDNSNSLKKMVSKPTARTLIVPELTALAPYFVGIKFRNFSLPESKTYNHVFSFSDRTLLLLLRDKTKLVSILKHNRRGMMRVYPSVVRFRSDNFNPITFWELGCQMAATNWQIWDCGEELSESLFASIGSEMASGLGYSGYRLKPKNMRNLEMNTSTYDKEKLKVQTLSSLVFDKEHFFDVSILSGQQLPKPKDFGNTGSDGYTPWVEVEVYNVLPVHAEIVHLHRYNRAGTLDEEKDDIEDEKDGEVGEAENEKEETRKEDEFNISTDKQQAIENASDGKINSKLTKAGSVKLSHVEAMSANPNHSVLFKTRFAENPGNAFSPKWNASCRLKYLTNENDLAFIRVAVKTLRASKTKSGVIGKVSTVVKNNNNDYTIGSWCCKIGDLKQGYRHIRLGDNKGEELIYSSLFIKIETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI25</accession>
    <name>A0A1E3NI25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PQ-loop repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73275</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016884.1">
        <property type="nucleotide sequence ID" value="XM_019164430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI25"/>
    <dbReference type="GeneID" id="30181117"/>
    <dbReference type="OrthoDB" id="19344at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF11">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="32051" checksum="A8CECCEBB19A2EC7" modified="2017-01-18" version="1">MEIAKKFSCKVYQQPDVFTFCLGFLLAIGIFVSYVPQHIKVIKRRSSEGLSPAFLLLGSLSAFSALMNIFIVTIPARRCCVAQLNTYQCANSLVGFVQILLQAVGYILIFVLCVFGTKHSIRESKTNHHKLTLNFYIFVASALLDVLFVVYLFQIDKKGGMESLLFFADISGVISTVLAIIQYIPQIYTTFMVKHAGSLSLPMMCLQTPGGYVWSYSLYSQPNSQWSSWLPYFAAANLQLVLLLMCVYYKMYFPTKLTEANAELRIAEENMSHSISHADENTSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI61</accession>
    <name>A0A1E3NI61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleoporin NSP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear pore protein NSP1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoskeletal-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_17089</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoporin NSP1/NUP62 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016935.1">
        <property type="nucleotide sequence ID" value="XM_019161458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI61"/>
    <dbReference type="STRING" id="763406.A0A1E3NI61"/>
    <dbReference type="GeneID" id="30178145"/>
    <dbReference type="OrthoDB" id="344345at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000040">
        <property type="entry name" value="Nuclear pore glycoprotein p62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026010">
        <property type="entry name" value="NSP1/NUP62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007758">
        <property type="entry name" value="Nucleoporin_NSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084:SF0">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05064">
        <property type="entry name" value="Nsp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin NSP1-like C-terminal" evidence="16">
        <location>
            <begin position="441"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="501"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="67"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="131"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="285"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="651" mass="66829" checksum="C254F27322DBB858" modified="2017-01-18" version="1">MSGNNTFSFGSGSNSNSLFGNANKDNTSKPAFNFGSASNANSATNPSSGGFNFGNTPTPNTAATTPSSNFGFGSKAEATSGTASNASKPFSFGSSSNTAGAPSFSLNSSAAPSKSLFGGAGASSTAAPQKTPGSSLFGGSTTSSTPFSFGGNAAAAATATSTNTPSSSTAPAATAPKPFSFGSSTTPTTAVPASNSTFSFGASGSNAASSQTQKPAFSFGSNSAAAEPKKEEPKPAFSFGSSSSGAPTTGTPSTAEKKNTTSGFSFGSTAQNANQTPSTTSTSKPAFSFGSGTSSTTPATSAAPASKTSFSFGSAASNTAPATSAASASKPGFSFGSTAPSTTPAATVTPASKPGFSFGSNSTAAAQDTPKTADSKPAFSFGSSASNAEEKKKAEPATTKSVGFALDSKNDTAKDSTEKKSSFDISTASKDKVPVKLEDFKPQQLSIMNKSIEDLITRWTNQLSLSGKTFETYSERINEWDRILVNSSDTVSKLYNDTILCEQKQEKIDQTLAYIDKQQDELDRLLSGYERQAEKLSASIEKSAPSLASSISGNNKETNDEAREKAYKLAEMLESRLDSLGSNFSSLVSEVNDVSVNFNKTLMNTESSGEGEQTLNDILKLLNNHMESLSWISENEKHLKEKLEKLSVSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NID3</accession>
    <name>A0A1E3NID3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_59488</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-serine + hexadecanoyl-CoA + H(+) = 3-oxosphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:14761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="EC" id="2.3.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016997.1">
        <property type="nucleotide sequence ID" value="XM_019163371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NID3"/>
    <dbReference type="STRING" id="763406.A0A1E3NID3"/>
    <dbReference type="GeneID" id="30180058"/>
    <dbReference type="OrthoDB" id="65434at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF3">
        <property type="entry name" value="LD36009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="162"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="571" mass="63999" checksum="2785263CC70FD16A" modified="2017-01-18" version="1">MKDIYDISELSPHVPDPPVSHMTVEQKAEMEFGPLTSDKWLSYAQHDPNDDIKPSVLDDPPYFIVLTTYLDYLIFILIGHIKDFFGMTFMPQHYSAMVTKNGYPPWYSAFESFYPRRLKRRLDDCFAIPIHGVPGRYINCYNRATPDYNETFYYDGTSRPSLNLSSYNYLGFAQSVGICTDDAVSSVQKYGLSSCGPRNVIGNTDLHVYTEKILSRFLGKEDSCVFSMGWGTNAHLFTSMVNSKCLVISDSLNHSSIRFGIRLSGAAVKVFPHNDMDALEKILREAISQGQAKSHRPYKKIFVCVEGLYSMEGTYCNLPALVALREKYKYYLFVDEAHSIGALGANGRGICEYYNIDPNRVDILMGTLTKSFGAAGGYVSGDRKVIERLRLDITSNTYGESMPPAVLQQIATSMRIIAGELNGNEGKERLQRIAFNSRYLRLGLKRLGFIIYGADDSPVIPLLLYNPCKMPGFCRKMLQHGVAVVIVGYPATDIISSRIRFCMSAALTKEDIDKILAISADIGDTLNLKFSSGIAGGEKNPGDWKKGIRPRWSIEEVLAKTPEDCKKKIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIE2</accession>
    <name>A0A1E3NIE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017012.1">
        <property type="nucleotide sequence ID" value="XM_019161487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIE2"/>
    <dbReference type="STRING" id="763406.A0A1E3NIE2"/>
    <dbReference type="GeneID" id="30178174"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF100">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="231"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="494" mass="56722" checksum="BDD7E85F4B2D49EC" modified="2017-01-18" version="1">MMQDKKRPKKNEYRNYKLPRSSNNFIDMIQLKDPKVTKEWFFETYIRPRKPVLIKTLNTDKVRIDIDNFRPGRILETLQGEQNEEESEQLHYLQVEELNQGGFGTGQKRLKLKLDQFLDRINKGESLYLTTQYSENHADPLSETASPITDGPGDEAEGPDDEEEDEDEDEDEEGEDIGGAFPALNGNLSDDDEIVDLHDDFDDVFDDVSDDDDHPDDDIGEPVDPIYQPPLSNLLNGKLEKLPPHPFELTESLVPQQINMWFGRTPEESTLLDIIESEQGSNIASINRGLPTPNSTSTGLHHDHADNIYVLAQGKKRFTLYSPDYAPDLYTVGDVRYIYDTGVIDYFRNEKAKNWSSIRADGGCIESYSNTVQSTETEEKVDPPSFSRIPPALLHLDQVDEETRKKLEKYTKDNFPLFYPVRNSSVKVTLEEGDLLYLPAGWFHEVTSFGDEDKNNVHVAVNYWFVPPDGSTSEQPYSDKVWEENFEMWKKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIG8</accession>
    <name>A0A1E3NIG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_34588</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017040.1">
        <property type="nucleotide sequence ID" value="XM_019162895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIG8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIG8"/>
    <dbReference type="GeneID" id="30179582"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000026">
        <property type="entry name" value="Proteasome subunit alpha type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="252" mass="27856" checksum="CE6660889394531A" modified="2017-01-18" version="1">MSGTAGFDRHITVFSPEGRLYQVEYAFKAINSTNLTSLGLVGEDSVCFINQKKVGDKLLDPTTVTYIFDISPNVGMLATGSIADARSLATRARSESAEYKYKYGYEMPVDVLTKRMANLSQIYTQRAYMRPMGVALTFISYDEELGPSLLKTDPAGYYFSAKAIATGSKQSEVSAVVERDFKKIVKKEPDSKVIVKGDWKKLVEYGIITLSNALSTEFRKNDLEIGVSTKAGFRALSADEIDERLVSIAEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIQ4</accession>
    <name>A0A1E3NIQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_59583</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017114.1">
        <property type="nucleotide sequence ID" value="XM_019163376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIQ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIQ4"/>
    <dbReference type="GeneID" id="30180063"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="23"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="159" mass="17965" checksum="A0ED804176215DFD" modified="2017-01-18" version="1">MQTAAPPLWRDTLPLCVQQLGVEKKKVTVRKFKNMKLIDIREYYQKDGKWLPGSKGLSLTEDQWNVLVSKMVDIGEALVKIDDKEQFNVMKAALDRKQQQQKEDEEAEEYEADSKDDASAAAVAAAAADEDEDDEDEDIGDVEFEDVSEAPAQKRVKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIT8</accession>
    <name>A0A1E3NIT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_73527</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine(1779)/adenosine(1780) in 18S rRNA + 4 S-adenosyl-L-methionine = N(6)-dimethyladenosine(1779)/N(6)-dimethyladenosine(1780) in 18S rRNA + 4 S-adenosyl-L-homocysteine + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10234"/>
            <dbReference type="Rhea" id="RHEA-COMP:10236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 9 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017165.1">
        <property type="nucleotide sequence ID" value="XM_019164506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIT8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIT8"/>
    <dbReference type="GeneID" id="30181193"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052909">
        <property type="term" value="F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.480:FF:000002">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000007">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 9 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="12">
        <location>
            <begin position="42"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="321" mass="36668" checksum="BA861343B4FF2DBC" modified="2017-01-18" version="1">MPKADKKTFHKNKPDPTGSAQINKVFKFNTELGQHILKNPLIAQGIVDKAQIKPSDVVLEIGPGTGNLTVRILEQARKVYAIEVDPKMAAELTKRVQGTPLEKKLEIIMGDFMKLKDLPYFDVCISNTPYQISSGIVFRLLEMPRPPRIAVLMFQREFALNLTARPGDSLYNRLSANAQMWSNVKHVMKVGKNNFRPPPKVESSVVKIEPKFPKPNIDYNEWDGLLRFCFNRKNKTLNSIFKNNKILDILEKNYKTYLQLLSEQKGDMIYDDSADYGEIVKQNVSKVMEETGFGDKRSSKLDQTDFLKLLYAFHQVGIHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIV7</accession>
    <name>A0A1E3NIV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TAP42-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017182.1">
        <property type="nucleotide sequence ID" value="XM_019161536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIV7"/>
    <dbReference type="GeneID" id="30178223"/>
    <dbReference type="OrthoDB" id="10261753at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45480" checksum="68D72D0DC2DB3576" modified="2017-01-18" version="1">MEQNSIASKYKTLNADLKNVQGRLTFEQFRKIHNEFIELSNLIEKADLFSENETLDEVSTSSLAYLLVSYKHGKFIDSNAVSSQLGVDAEPAKRNRLRLVTLQIVESMIWKFVSLVVLDLKIGDSLGEEDEKFKGIYKWITAYSDIRQREISSHGTKGFVKLNELELINFKGAGPMARRDMKIDKWNLEKALGQKVKLIGDTDDMVGKFDDEVVRKVRIDQLLLAVVESVSLLESNAMEREMLTNVQGSGDDLEQLNIEGMKLISPETEDKDSRKSQNKGSIFDKGYTDKLEFSDNDKKLLSKEGKVLRPFTIVSSDKQRKELQNKVFGTGQVLPSMTVEELVEQELQNGGMVKPKEEEPEIDEDDYEWQNKETRRLREWDDFTDSNKKGSGNKMGNIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIY0</accession>
    <name>A0A1E3NIY0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_54054</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017208.1">
        <property type="nucleotide sequence ID" value="XM_019163173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIY0"/>
    <dbReference type="STRING" id="763406.A0A1E3NIY0"/>
    <dbReference type="GeneID" id="30179860"/>
    <dbReference type="OrthoDB" id="10259785at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="576"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="696" mass="77721" checksum="9BD3FAD460927F9A" modified="2017-01-18" version="1">MSTRPQVSVVSIKGETTSTALPLPAVFKAPIRPDVVHSVFTKVNKNKRQAYAVAENAGMQHSAESWGTGRAVARIPRVGGGGTHRSGQGAFGNMCRGGRMFAPTKIWRRWHIKVNHNEKKYATASAVAASAVQSLVLARGHRIEKIAEIPLVVSDDLQSVEKTKDAIAALKALNAHKDVIKVIKSKKMRAGKGKMRGRRFTQRRGPLVVYAQDNGLVKAFRNVPGVETANVRSLGLLQLAPGAHLGRFIIWTESAFAALDSVYTSLPTNIISNTDVTRIINSDEIKAVVKAAGPSQSKKTASLKKNPYKNKQILYRLNPYAKAFSEKKLGSAKAPASKKADYAKFTEVFNSTTRANTMDMNAPTHESLPLSSNHSLNQSLDSRFATQDVLSSNSSIRNFESTMANEEYTQLTDLLNTNPPTPTEAFVKGISPTDSDDDDDTRPFIVINLEDLTMISSYPNNTTPNSFVYNADHSQMPNTAKEVPHDIEQAENYTSPLIMRHVIKQPDDIYLTSIVKAYQYPKAYHALIAYLKKRFNKRQLLEIAKCMAKYRPSFISATKSLYENDLIFTERSFQRTLLEYENLISMSPSPTIIWRRTGEIVALTNEFAVMTGYSRMSLLSKRTYIVELMDDESTINYFRSFSEFAFGDLNATYVTDCNLRKAEDDNYLRCCCVWTIKRDVFDIPMLIVGQFLPVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIY6</accession>
    <name>A0A1E3NIY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_12539</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017217.1">
        <property type="nucleotide sequence ID" value="XM_019159941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIY6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIY6"/>
    <dbReference type="GeneID" id="30176628"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="99"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="382" mass="43512" checksum="2BB6F984A035886F" modified="2017-01-18" version="1">MASGMQFLKQQLCCRGGCLGIGDQALSLAVQRLVHTSSKGLGQQRVCRRTYATVGGVDGDDDAGSRRNTKEGQKDTGNTELDHKILKLIHAYPQSPTATPYEVLNIASSDKNSVSGADLKKRFFQLAKIYHPDSSSAHGYPLERGRQSPALTDETKEARFKRVLSAYNLLKNPIAKANYDRYNVGWNDFSNLRTNANMYSPSSSEYRNFSGSNFYRSNFTSYETGTWEDRYRYGYGSAYGFHNDQSWSSSKTGDMKEELLKNKKTIFLSFVLTIAVYGALQLTHLYLYDDVIGEHYNESLSASSVNVHEKSEDDLFHAYTNYGLGDTKEDRINRFLWWRKLTMAFSLADVKEVLDHLYKRGIIENSEENTKLRRYDYEARKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJC7</accession>
    <name>A0A1E3NJC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_126855</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM54 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017360.1">
        <property type="nucleotide sequence ID" value="XM_019159960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJC7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJC7"/>
    <dbReference type="GeneID" id="30176647"/>
    <dbReference type="OrthoDB" id="5598305at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021056">
        <property type="entry name" value="Mt_import_IM_translocase_Tim54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF101">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11711">
        <property type="entry name" value="Tim54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="427" mass="49342" checksum="CEB08DC6C265F611" modified="2017-01-18" version="1">MSYSNPAFAALGIKSFKLPSRNWMIFWTVVGTLGGGIVYDKWKQRQLRAQYMEMYANKCANYPVDEVHRKLRIYVSPPPNDYLDESMKYFRRFVKPIINASGVDFELVTMERQGDIRYKVAEEIRALRREKAGLEPKKKKEEDDTGKEVSASTPAAAANINPVFQSYKVENPKNEEGEIKAVRDLYEPMDVLGVKRVFGDFHQRVENVKSEDALVTNVRDAGGIICIGRGAFKEYINGVHEGLLGPLEEKEKEDDEKYAPAAYIYAKDYKESTIAPELGLDKVDFNDDVQFSRVLQTLRDEKSGVPYFFVQPILELRNYNVSGFTRQPERMWRFYHKREQLIEYKDKVVELIERKWCAFTRSDLDNGIEEEGDWPSSWLKVAKKNNSEWIREFDGDDRVMKVLASYNCKCDKDAAPDATSLVDETQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJG2</accession>
    <name>A0A1E3NJG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_58630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017395.1">
        <property type="nucleotide sequence ID" value="XM_019163309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJG2"/>
    <dbReference type="GeneID" id="30179996"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133403" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12221" checksum="563866D3D55B169A" modified="2017-01-18" version="1">MGMGSTWLLALRFVSPWSALASPRTMTSLRPVAHLAADRRSRLTVLRCTCPGSISARASAVWRPLYQTPATYQNKSPFQAGVSVQLTRRRPEDYTSFDRRGTTPFATTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJJ6</accession>
    <name>A0A1E3NJJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_33235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lcsJ thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017359.1">
        <property type="nucleotide sequence ID" value="XM_019162808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJJ6"/>
    <dbReference type="GeneID" id="30179495"/>
    <dbReference type="OrthoDB" id="265761at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051490">
        <property type="entry name" value="THEM6_lcsJ_thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475:SF4">
        <property type="entry name" value="PROTEIN THEM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="223" mass="25869" checksum="D7E510F019AC85EB" modified="2017-01-18" version="1">MIGKLILGVVAIVCVSSYKYIFGAYSIRFFWIVVKYLYLKKLEDLDDKGQAKSPFVELVRTTKCCLLECDFFGYHKNNVTYFTELDLCRTECTLNALHRYFKLAVLKKESLAYVPLASICNHFMKETKPFENYEMRTKVVGWGDKWLWLITVFTGDKVGKRIYCLSLAKLVFKAGRKTLQPWDVVQLAGSTDPDVCKKAYRNEERVAQNTNNVMQLLKVYEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJK8</accession>
    <name>A0A1E3NJK8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16227</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017440.1">
        <property type="nucleotide sequence ID" value="XM_019161129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJK8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJK8"/>
    <dbReference type="GeneID" id="30177816"/>
    <dbReference type="OrthoDB" id="5894at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="432"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="566"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="263"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="517"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="68797" checksum="0F60B953D64DDEB7" modified="2017-01-18" version="1">MRTCYYELLGVEQTATHIELKKAYRKKALLLHPDKNPDDVENTTRLFNDVRVAYETLSDPQERSWYDSHKFQILMEDGDRDSAAGYDDDGDNYSTYYAGTTVEDIEKYFSNTLYQNFNDSINGFYSIVSVLLDKIASEEVSAGKQQKLPGFQDFKDDTSFATVCDPKELMYPRMGNSKTDFEMTRIFYKVWGNFQTVKTFNWIEEYRYSTAPDRRTRRIMEKENKKLRDQGRKAYNDVVKKYISYIKKMDPRMDPVIRKKYEKQQLALKRQELKKQAQLEKEERQRQRQLFEEQEWQTVDPDDLAEIEAQLDKIHEEERKINGQEADDEDHDEEIYECIICDKIFKSEKQFADHERSKKHLKLLKQLKWEMKKEGIELGIDNDGYINDNGEEMDNDESDDEDFEDFDDALESLTELDELTNEELQYIIDQQISQQNDDNANANKSKDVADIETPDQSSKSIVDVQLDDDIDEESHVDPATADPQEWKTDKKHEELSKLLNGMGIESDDSDDWGGSKSKVRTKKGKNKSKKAGVKASDNSQDNLSVPTETEKCSVCSESFASRNKLFQQVNSTGHAASPSKVKKGKKKEKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM0</accession>
    <name>A0A1E3NJM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72388</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017423.1">
        <property type="nucleotide sequence ID" value="XM_019164186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM0"/>
    <dbReference type="GeneID" id="30180873"/>
    <dbReference type="OrthoDB" id="446113at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12344">
        <property type="entry name" value="RRM1_SECp43_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="53"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="170"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="425"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="247"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="364"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="250"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="364"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="640" mass="71114" checksum="6E6105A3B9E87D7B" modified="2017-01-18" version="1">MYSNNPSYDAHSNQASLNTMNTMNTMNSLSNTVNTPTSTSSSQTSINTNGQIPQLWMGELDQRWDELTIKQIWTSLLSSMGIVVHSVKLIKDKQSSQLGLANAGYCFIRFHNFEDASKVLNTFNGKPIPGTNNQHYFRLNWSSANIQAAAVAAAATSTLPLTASSNPVEYSIFVGDLPQNINEQILLQTFKSKYPSCTSVKIMVDQTTGNIKGYGFVKFLNEDEQKKALVEMQGCVLLGRAIRVSTASKSNSSLPNTNSGNNNGNVTSSNNNNTFSPLLSTTLTSHSNVSNTSLNTNMSPSTMMSSNSSIPANINAGIPQQQQQLHQQQHQQHQQHQQQQQHQQQQQQQQQQQQQQQQQQQQQQQQILNKFNSQQHQPQSQSQPQSQSQQQQQPGNSPQNLSNRSLLQPLPQNTSVQYFNDPANTTVFIGGLNVPFSEKQMFDLFSKYGNISYVKIPAGKNCGFVQFFHRASAEMAINEMQGYDIGGGCKIRVSWGARAAQRSWFARQLQLQQQQQQPQQQQHLSLSHLAQIPAPASSTSSSSSTQNLDQLSNNDINVLLNMSKNQSMADSLGLFDSYVNVNDTMLNLNGYSVDQYNFQNEQIPYSSMSSNDELKLNKLLLAARDGNLDQLDLGSNLYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM2</accession>
    <name>A0A1E3NJM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Haloacid dehalogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017458.1">
        <property type="nucleotide sequence ID" value="XM_019161134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM2"/>
    <dbReference type="GeneID" id="30177821"/>
    <dbReference type="OrthoDB" id="2363873at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF4">
        <property type="entry name" value="ACID DEHALOGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27163" checksum="C25B12C94CA5CB71" modified="2017-01-18" version="1">MPDIVFDIIGTCIGYDVMLDAISTRLGDRLLEHNINVELFFNAWGTACERDFSYLSQIGHYQTTRKILRATFYRTLYHAGIQDPKAFASEDDLDFLSSEWLKLKARPELPALWKLLRDNGFTVWCLTDGDQERVKGYFRNSNLEMPDENVISCDSLGVGKPTPAVYEYMLNKLPNHGADSWFAAAHQWDCCAAKSAGFRTAWTNIYEKYPGEDIFGLPDIVAEGLLDLGEKIVAAQKNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJT8</accession>
    <name>A0A1E3NJT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016726.1">
        <property type="nucleotide sequence ID" value="XM_019161395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJT8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJT8"/>
    <dbReference type="GeneID" id="30178082"/>
    <dbReference type="OrthoDB" id="10264707at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052742">
        <property type="entry name" value="Mito_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138">
        <property type="entry name" value="ACETYLTRANSFERASE, GNAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE ACA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="54"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="207" mass="23409" checksum="B37216791EF120DF" modified="2017-01-18" version="1">MVVEENRFITQPLYDELRDGTPVTILPYNSLEETPANVVDAIYTLFNAIVEEGTTYVHEHPLTRTQFYEYYFPHFVAVMVRGTVPEQDRLRTDLDIAAGGAHELLGCFYIKPNYIGRSAHVCNAGFLVSLDHRGAGCGSVMGRNYLKWAPLLGFKSSVFNLVYETNVASCRIWDRLGFERIGRVKNAGRMKGHPDLVDAIMFGYQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJW9</accession>
    <name>A0A1E3NJW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_33687</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017523.1">
        <property type="nucleotide sequence ID" value="XM_019162835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJW9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJW9"/>
    <dbReference type="GeneID" id="30179522"/>
    <dbReference type="OrthoDB" id="1737613at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004127">
        <property type="entry name" value="PRK05617.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="496" mass="55516" checksum="F767FD350B52E625" modified="2017-01-18" version="1">MATAASKENLVDFNVLGNVRTVTLNRPKKLNALNTEMCTEIIPRLVEYSKSKSASLVLIKSSSEKAFCSGGDVIQCAKNNMADESPKSIEFFQNEYNLNYLLSIYGKPVVSLVNGIVMGGGVGLSVHGSFRVVTDSTRFAMPESSIGFFSDVGTSYWLPKLDANLGYYLSLTGDELTGYDTLLMGFGTHYVPSCRFPELISRLSEIELPDLSLDRRNSKVFNHNNSSQLFAIVNSAIEEFVEEIPRTHKFKYTPEQLNTIEKCFNPKTNKFVEDIINSLKEDGSEFALETVKKLSSKSPISLNLNWNMLSRNRNATIQESLTRELRLAAKLMTNYRNNDFNDFIKNKLIEKKVEIIPSQYYPTLKDVPSSIVDDLVSLDVYNPQSSGGSESKESLENLIETLNNLKISNFSELGESVANFSKSPHHMGLPSQYEIESYVKGSKNSSNLPPVTFAETVNYFRTKYNEKAGVTNKVKLVLNRKSHPSSVDEGCIEWTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJX0</accession>
    <name>A0A1E3NJX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_16269</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017489.1">
        <property type="nucleotide sequence ID" value="XM_019161144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJX0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJX0"/>
    <dbReference type="GeneID" id="30177831"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="12">
        <location>
            <begin position="406"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="287"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="526"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="526"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="555"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="626"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="665"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="675"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="845" mass="91377" checksum="02E913793DBE01BA" modified="2017-01-18" version="1">MAQTNANSEMDLSGNPMASMLMPTHPGGGAAAATQDDQELVDLQTQQAAAPTDAGGSLSSAVTAAVQQGQSTDFVKKLFQMLEENRFSSIVRWTDSGDSFMISDTNEFTKEVLPCYFKHSNFSSFVRQLNKYDFHKVKLSQEIRQRYQLENVWEFKHPDFNRFDKSSLENIKRKVPAKKEQTDTVAAANCVPITSYRKLEDRVSFLEKENVSLNSSINNMSVSLNKLNQKYNTLVSNLLTSKDIDMSFSRSIQILSKGLVQLGVQLPAIELPHLDYLADTSDTLSSLNTHNSSSAANPHPAAHPPVNGQIATNTGPHLNNMGNFNNHSASAINNAPPPTQNNQLSDSHTGSNRRSSQNSLAGGKMMDVEIKQEEEVAEPELKPTPTNSALNPQARLLNRPQGSALHVLLVEDDDVCIQLCRKFLMKYGCTVVVVTDGLSAISAVEQVKFDLVLMDIVMPNLDGASATSVIRSFDKDTPIIAMTGNYQRNDLVTYLNHGMTDILAKPFTKDDLYMILEKHKMDRKLNFTNKSPDDDISSRQAQLTQPQPPIHQHPSMPSLTSQAPTPHVQLSAQQSSTQLPQTTVDGQTLVLASQTNSGQSNPESSNTPSGGMTRNPALKSVQPFDNDTVNSSSNNNSNNNNTTPANTANTNNIANIENNGNVSNTNQNYNSDATNNSISNGNPSSNNSEGGNKLVPLGDQLVSSNLPPLNERRITQARLVKKYGNSLLNSASSMSAGQMVQEMELNVANIPNVHDDLLTPTLLESPGLTPDAGMPGDVGDSLSAAAAVEAVTGVTPAGHQAGMSLGNQPPNVTASASDALNAAEMDISGVNDAEFGDMFKRRRVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJX9</accession>
    <name>A0A1E3NJX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_129177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017499.1">
        <property type="nucleotide sequence ID" value="XM_019159986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJX9"/>
    <dbReference type="GeneID" id="30176673"/>
    <dbReference type="OrthoDB" id="10581831at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="346" mass="38694" checksum="E97A9F93277C824A" modified="2017-01-18" version="1">MDPKVAFSSVDAVGSRRGKKFASTVPSLNLSTIHVILLSVIFTLLQNISLSRLPGASSAVFVVAMCLVSVLGYYVIHHLPLTTSMCRMKVRLALTTLQVLTKDLKFDLWVTVIAVLLVAWVLSIWKTGVSFLIWCFVIWAYNLDETKVNSMSNNSAGAGGGNGSNFISAFASFSNLSTGTSNASGRFSSFEDFGPASGLGTTANSDLNSSYKGTPDYDKCYSASIGYINSLEKKVIFLEKKISSLENDKLEQNIIIYTFNHNLNEKLNNLEKKVQERDQTSQQDLNQIYSMLLALKKRKRIRLNILKLYIKQILLWLLPHPVVVVLRRTRRIVNMIFQRLRGFFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJY1</accession>
    <name>A0A1E3NJY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sterol 3-beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.173</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Autophagy-related protein 26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_34457</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22724"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:37424"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.173"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:22725"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D-glucoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61836"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16933"/>
            <dbReference type="ChEBI" id="CHEBI:52973"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:61837"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.173" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016766.1">
        <property type="nucleotide sequence ID" value="XM_019162886.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NJY1"/>
    <dbReference type="GeneID" id="30179573"/>
    <dbReference type="OrthoDB" id="10261837at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016906">
        <property type="term" value="F:sterol 3-beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13215">
        <property type="entry name" value="PH-GRAM1_AGT26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13216">
        <property type="entry name" value="PH-GRAM2_AGT26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000303">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000029">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000009">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048066">
        <property type="entry name" value="ATG26_PH_GRAM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048065">
        <property type="entry name" value="ATG26_PH_GRAM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010610">
        <property type="entry name" value="EryCIII-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050426">
        <property type="entry name" value="Glycosyltransferase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050:SF25">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06722">
        <property type="entry name" value="EryCIII-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="11" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="20">
        <location>
            <begin position="371"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="32"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="600"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1316"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1316"/>
            <end position="1330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ45653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1338" mass="149508" checksum="6455A9847C58D78D" modified="2017-01-18" version="1">MVTPAQHLLSSALRFPKGAKRSLIDKNLARLRSHEETEDGGIDDERDLVNDLDREDDDSLSLVSDNNSDYEGQNEDATAIRNSDSDNDDEDGEDGISDLRKSQGKELSPENTRSRLSLRHRRTSSSASSRSLEARRQAMSPQRFINSISKHSLSPIKSGVAAIGSPIMHRISSDKVEEPTVEGISRSFAGFLTAASMYAGFQDIEDEDREVESIRNKDFTSADSSTDGELGPPDDATHLQVLDNDDDSDAISGISTPTDTPSVYSGNTIAPAEGKHHVTFDGDGINSKFEVSVAKKVTDNAKPGDQPEVLENKAHAISRKLRETFDISQSEAFIGDYPCWLMGDVLLQGHLYITDNNILFFAFLPKRNKGGISKSGSLSVKSFHSLRMHRKWIILRENTFSVYSSPKDLYFPELVIDLRTALRAEIYGSSKNSNPVVPVWIRIVTEKRTHWFQADNHDVARSWVTILKKHIFSSRNKGDQVAIKIPLQNVIDLELTSVVGVTKNLRIKVIENAESFAIDDYFVMFFSKGDAAVEDIKSAIEKAGVVISSSLIDDNDDLRNSDLVKSKIEIIKKSCSSVKLTNFKPKEKKGKLKGFRDSILSSDEDEGNEGDDEIDDQFGTAEDDEATNVQKHEFIPSKAKSLISSRLRGRSKKPHRWTSSIVQGITSFTQSLIFSGPVMHFDEESSFVKEGEDPFFVRDQAKRISCQKRFINRFSLNESEKLIATYHLYLLKGLPTYGKLYIGSHEICFRSTIPGTSTVMILPMADIENVNKESGFRFGYFGLVIVIRGHEELFFEFSTHDARDDCEVQILKNLDSYKRLSAESSATENDIEPNLASSAGSLNSARLKMFESKLTDAVGLNVPIIVEEHPLKQAEFKAFKPYRFTLLTIGSRGDVQPYIALGKALLKEGHKVKIVTHAEFKGWVESYGIGFDVIAGDPSELMSLMVSHPSINYSFIREAKAKFRDWIDDLLNTSWKACQDCDVLIESPSSIAGIHIAEKLQIPYFRAFTMPWTRTRAYPNAFMVPDQKLGGAYNYMTHVAFENGYWRGTCYQVNKWRVQTLGLPKTNLGAMHQNSVPFLYNMSPVVFPPSVDFPEWVKVTGYWFLDESASYVPPKELTDFIKKARDDDKKLVYIGFGSIVVDNPKELTQAVVDAVLESDVRCILNKGWSDRLGSKNKNDIEIGLPDEIFNSGNVPHDWLFTQIDAAVHHGGSGSTGASLRFGLPTIIKPFFGDQKFYANRVEDLGCGVALKSLESKHMAKALREITTNKRIIEKAKVVGEKIRSENGVQNAINAIYVLMDYATKLSISKHKQDLADDEQEGFDDSNDGTDIDGSWLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJZ3</accession>
    <name>A0A1E3NJZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_11460</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017575.1">
        <property type="nucleotide sequence ID" value="XM_019159799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJZ3"/>
    <dbReference type="GeneID" id="30176486"/>
    <dbReference type="OrthoDB" id="413400at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133407" description="J domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="47"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="349"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="265"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="386" mass="44770" checksum="72671C5FA6D5DB4C" modified="2017-01-18" version="1">MQHLRFVYLLLLAGLALAYFSPTQLEILDLHYKIQPKQASKSADNRTFYSLLSISPSTPEDQLERAYRKLSRKWHPDKFVMAEPKERRRAERKFETLSLIISILRDVDRRKSYDYFLKNGFPVWDESKARYVFQNRFKPNFTLVLVFLAVLTSIGQMIILKLNRSQKNKRVEQILRDVRWKADNMNRSSADPNKVMELPDDYELDSNFSGYSVDDKLVTYCGKVFIVKPDRSVLLYNDEDFNAEDQQSMNDLTKQIVESGHFNLAGFQKKEMNRKERRYAEKQNKKEAQSEIDNVISNLVRFQEDDSGLKVTDLYLVKAVLKLWSISVGQLFGKSSPASAKEAVVTEEQVIDTETESHNTPLQDNAENADGKKVLSSGKVLRSRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK09</accession>
    <name>A0A1E3NK09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16302</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017529.1">
        <property type="nucleotide sequence ID" value="XM_019161156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK09"/>
    <dbReference type="STRING" id="763406.A0A1E3NK09"/>
    <dbReference type="GeneID" id="30177843"/>
    <dbReference type="OrthoDB" id="1928736at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048025">
        <property type="term" value="P:negative regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="4">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="97" mass="10524" checksum="C5D652379D3A0D83" modified="2017-01-18" version="1">MNAKLALAIKSGKYTLGYKSTIKSIRQNKAKMIIIAANTPVLRKSELEYYAMLSKTSVYYFQGGNNELGTACGKLFRVGVLSILDAGDSDILSVVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK20</accession>
    <name>A0A1E3NK20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_147178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017021.1">
        <property type="nucleotide sequence ID" value="XM_019160496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK20"/>
    <dbReference type="GeneID" id="30177183"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="146" mass="15625" checksum="1084982C4AB5B9E6" modified="2017-01-18" version="1">MRKAKTRQGRKTEKRPKASSMRVHVTRPQPRFVLLILRIAVATTWPPVSRTTTQQRGSSAAAGTAQILYCTGVGIAKVCVVFRSPHDRGHASAMAIPNASAISTSCHCPHHRLLAVSAARLLTAKTTAISVFRFAVFSPPGGCLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKL0</accession>
    <name>A0A1E3NKL0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_58914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017773.1">
        <property type="nucleotide sequence ID" value="XM_019163328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKL0"/>
    <dbReference type="GeneID" id="30180015"/>
    <dbReference type="OrthoDB" id="3997928at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015159">
        <property type="entry name" value="Rec107"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09074">
        <property type="entry name" value="Mer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="271" mass="31480" checksum="FA5B0114D83E4134" modified="2017-01-18" version="1">MKHKCFRELELQLLQPQAFFYYRSFSKNPRRKKDLSMNSDSTDISSDVETTAVEADTFDKQELIVWASKLELESIDYRTTSIDLLEKLSKLSKQLTESLGSVKTQIFEMKINGSEKDRKLKNFIELTVAGEVDEVYEKISQVSNKIKELHGSVRAIEALDLESIERKFNAFLKIQNKKNKEYEEQLQTLQLLLDQATKRTPQRPTPPFEVTAEIGTEKISQRCKPSRPNKEDGKLNAYAKRSYSSKRPDSKVIKHSQQRRTSTFTRKLIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKS9</accession>
    <name>A0A1E3NKS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="13">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_16583</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="13"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017868.1">
        <property type="nucleotide sequence ID" value="XM_019161267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKS9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKS9"/>
    <dbReference type="GeneID" id="30177954"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF57">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="14">
        <location>
            <begin position="37"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="490" mass="53866" checksum="777189888768B599" modified="2017-01-18" version="1">MLSRLVSNSARKNAFSTIKRSYALSAQAQALISKNVKDIDPEMYEILQLEKARQKNSITLIPSENFTSQAVMDLLGSAMQNKYSEGYPGERYYGGNEFIDMAESLCQKRALEAFGLNPEEWGVNVQSLSGAPGNLYAYSAILEVGDRLMGLDLPHGGHLSHGYQTPSTKISYISKYFQTMPYRLDEKTGLIDYDMLEKTAVLFRPKIIVAGASAYPRVIDYKRMKQIADKVGAYLLSDMAHISGLVSAGVTESPFPYSDIVTTTTHKSLRGPRGAMIFFRKGIRKVTKKGKEIPYDLEKKINFSVFPAHQGGPHNHTISALSVALKQAQTSEYKEYQADIVKNASVFADALANKGFDLVSGGTDTHLILVDLRSKNIDGARVEAVLEKINIAANKNTVPGDKSALFPSGLRVGTPAMTTRGFLADEFKAVADYMDKAVKIAIKLKAKEEGATPKEKLAHFKKLAAEDPEVQALDKEVVEFVSKYPVPGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW8</accession>
    <name>A0A1E3NKW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_33626</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL41 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017908.1">
        <property type="nucleotide sequence ID" value="XM_019162831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKW8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKW8"/>
    <dbReference type="GeneID" id="30179518"/>
    <dbReference type="OrthoDB" id="408933at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019189">
        <property type="entry name" value="Ribosomal_mL41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09809">
        <property type="entry name" value="MRP-L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16288" checksum="BFEA9EA893B0B78D" modified="2017-01-18" version="1">MFSAARTYTRGFHTSTPALLKRPWKTYKDGTLFYGQSKAGNKRLALSTKQGNKNFYKGTRSSGIGHISNKGKYTIDYQRVRTFVTPADMATPLKPLVSASVPVPKNTFKGYTGITDGRLWLDNVKEYIETGDVEFEKTKTGVEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKZ3</accession>
    <name>A0A1E3NKZ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017917.1">
        <property type="nucleotide sequence ID" value="XM_019161284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKZ3"/>
    <dbReference type="GeneID" id="30177971"/>
    <dbReference type="OrthoDB" id="2898509at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="146" mass="16422" checksum="F0C2D8F9A57A2544" modified="2017-01-18" version="1">MAYPGAGQLGTPDDLNQEKSYSAYRCHMNTVAGNEAIVVHYSDNEHFNIYGLNPGIVKTNIRDNYLGKNSWVSKVLESFIGWFNKSPEQYAEIITPLLTNSALDADNGSFYDNNAKKIPASKGFDKEYADKYIRKSKELLDSKGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL85</accession>
    <name>A0A1E3NL85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Chorismate mutase</fullName>
            <ecNumber evidence="4 15">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_73577</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the Claisen rearrangement of chorismate to prephenate. Acts at the first branch point in the aromatic amino acid pathway where it steers biosynthesis towards phenylalanine and tyrosine, and away from tryptophan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13898"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017216.1">
        <property type="nucleotide sequence ID" value="XM_019164521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL85"/>
    <dbReference type="STRING" id="763406.A0A1E3NL85"/>
    <dbReference type="GeneID" id="30181208"/>
    <dbReference type="OrthoDB" id="191918at2759"/>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072545">
        <property type="term" value="F:L-tyrosine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120284">
        <property type="term" value="F:tryptophan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.590.10:FF:000002">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.590.10">
        <property type="entry name" value="Chorismate mutase, AroQ class superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008238">
        <property type="entry name" value="Chorismate_mutase_AroQ_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037039">
        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
        <property type="entry name" value="CM_pl-yst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF12">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="16">
        <location>
            <begin position="146"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="260" mass="30205" checksum="B21205877D7B1370" modified="2017-01-18" version="1">MNFLKPESVYNLSNIREDLIRMEDTIIFNLIERSDFPCMPSIYEKNSAQIKIPDFDGSFLDWLFQQHETVESQVRRYQSPDQYPFYPQVVKKSFLPAIEYPKLLAEYFREVNVNGEIMDVYINKMIPRVAKDVGDECENFGSTALVDIFTLQSISRRIHFGMFVAEAKFQADREKYTALIERRDVDGIMANITNSAVEENILKRLMEKAKAYGTDPTAGNQGTSKVHPEAVVGIYKDWVIPLTKKVEVEYLLRRLEDKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLK7</accession>
    <name>A0A1E3NLK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018145.1">
        <property type="nucleotide sequence ID" value="XM_019160638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLK7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLK7"/>
    <dbReference type="GeneID" id="30177325"/>
    <dbReference type="OrthoDB" id="346839at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000346">
        <property type="term" value="C:transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071667">
        <property type="term" value="F:DNA/RNA hybrid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990119">
        <property type="term" value="F:RNA helicase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="84"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="195"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="245" mass="26783" checksum="194C4B1BDBFF883C" modified="2017-01-18" version="1" fragment="single">MSSLEQSLDAIIAHSAKPGRKPVRRNVKGAKRPQVLSKTVVARPRTGLRKTVARPTAPVARRPVAPPAAPQPRLFQSASLDVATKVVVSGLPKDIKNDVIREFFQSTIGSVQNLTLAYNEKGRSTGVATVIFKNAKAAQAAVSRYNNAAIDSGRSKLKLELVVDTTKVPLAARIQPNARPATLQNRIQPPRKARVEALKGRPGRPVNRPTKIANKRKETPKKPVKKKKTLEELDQEMADYFATND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLL4</accession>
    <name>A0A1E3NLL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pyruvate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_71161</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018148.1">
        <property type="nucleotide sequence ID" value="XM_019163848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLL4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLL4"/>
    <dbReference type="GeneID" id="30180535"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050177">
        <property type="term" value="F:phenylpyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000950">
        <property type="term" value="P:branched-chain amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000951">
        <property type="term" value="P:L-methionine catabolic process to 3-methylthiopropanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="12">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="10">
        <location>
            <begin position="216"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="11">
        <location>
            <begin position="413"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ47035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="63175" checksum="B747B80A09E62DE1" modified="2017-01-18" version="1">MALSEYIFRRIESLGVHNVFGVPGDYNLSLLEHLYSVPTLQWVGCCNELNSAYAADGYSRTIGHDKFGVLLTTQGVGELSALNGVAGSFAEHVPVLHIVGTTKHSVKNRGAHYHHIINGVSTRDPTNHYVYEQMAANVSCKVLSLTDDLSNAANDIDDLFRTILATKKPGYLYVPCDLVNLEIDSSNLADVPAQVLISRMPSASPDAVEKISDMIVGKLTASQNPVVLCDVLTDRFGQTETLQTLVDLLKVPSCNSHMGKSLLNESQPWYIGDYNGAESNKTVQAYVQQTDCFLHVGDYYNEINSGHWSLYDKIQPESIVLLNPEYIKVGNQMFENVSFEDILPTVLQKLSSVDSLPTYNIPKTVFQIEEIPSNTPISQTRMLETLQSFIQPNDVIVTETCSLMFGLPDIRLPNNTKVIGQHYYLSIGMALPCSFGVSVALAEMKQTDSRLILIEGDGAAQMTIQELSNYNRANVVKPLIILLNNNGYTVERIIKGPERDYNDIRPDWKWTQILQTFGVSDAKTAKVSTPQELDNALKQIGNDTTVPRLIEVTLDKLDVPWRFHKMVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLM5</accession>
    <name>A0A1E3NLM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15042</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018150.1">
        <property type="nucleotide sequence ID" value="XM_019160640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLM5"/>
    <dbReference type="STRING" id="763406.A0A1E3NLM5"/>
    <dbReference type="GeneID" id="30177327"/>
    <dbReference type="OrthoDB" id="28947at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="606"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="523"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="919"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="924"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="943"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="985"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1001"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1087" mass="124110" checksum="BCD3E76C4BD403CB" modified="2017-01-18" version="1">MEGSLSNLASLREKVSNALSKKDEASKNNSKSKKNPKSNSKNNKEPSDKPAKKHVENKTPNDDDEQEILRREALNLGATEDDLKLVNDLDVQDESEQEFDDNDSKNTPNKELTKELSEFMKNIGFKSHKAEDLEVSDEEDTDAADGEAEEEEDKFEEEEARVEHDSDEEEADAISENEEEEEEEEENNLANSAQDKNETDKTATQSKSSDKILNLQSVISDRLAVESRSDWYNISQNLEHVVADRSMKQSDVEELYEKAKRLIEAENQVYDEEFNKSSSQKRFLSQILTGGTLSDKISALTLLVQEAPLHNLRALDNLFNMCQKKSRTAALQCVDALVDLLVNTILPPNRKLKTFKKQPLSKSLSDSQLILMYFEDHLKTMYFQFISLLEKLSHDSILYVRMKIVSHIFSLLKAKPEQEANLLRLGVNKLGDIDNKVSSKTSYQILLLEQEHPAMKQVVCESVIDVVFRSNNDHHALYYSVNTLNQTILTRQDETLANKLVDAYFALFEKLLIKTDDENVGAFKESEHQKEGRRKKNVKRGRKGGKSVKKVKDDNEILEEKNSKMFAAILTGLNRAFPFSNFPTNIFENHLNILYKITHSLNFNTSVQALILIQTITNKFSDDTHKDRYFRTLYESLWDERLLNSSKQGIYLNLLFKSLKDDENIERVLAFVKRISQVCLNWLNIGTISGMIFLLIQLEREIPQVRNLFLNTPMDDEINETLQQEESLDEVVSENKEVRIYDPRKRDPKFVNADKSSIWEIGFFLKHYHPTIQMYAENFLKFDELSKAERRTINKPDLGLYSLSHFLDRFVYKRPKSKANLKGSSIMQPLGGAHTGNLLVRATGVESSDIPANTEDWLSKKASQIKPEDDFFYQYFSTKQEKVMSSKFDKALDQKAKNGDEDEDSELDDDAVWNALVASNPNVEGEDDDLDDELSDLNMSDFSEAEDEEEEGELDLEGGDEEGNSDEDEEAEEEEGLVRLRTIGDLDDEEEVEDESNDENSGAKEESDSSDGEDIKMLLEDDAEEDLYSSEDDSVEPKPALTNAKKSKKRSNLPEDGKKNKKIKISSLPTFASAEDYAEYLQSSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLR7</accession>
    <name>A0A1E3NLR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_184950</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ47006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018119.1">
        <property type="nucleotide sequence ID" value="XM_019162024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLR7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLR7"/>
    <dbReference type="GeneID" id="30178711"/>
    <dbReference type="OrthoDB" id="5398665at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045895">
        <property type="term" value="P:positive regulation of mating-type specific transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006856">
        <property type="entry name" value="MATalpha_HMGbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04769">
        <property type="entry name" value="MATalpha_HMGbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Alpha box" evidence="5">
        <location>
            <begin position="71"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="178" mass="20921" checksum="42F28FC5411779D5" modified="2017-01-18" version="1">MTRSERKKKVECKRMRVNKQRPTNGTGKISKKTEIFIGKPTPTYHMPSSLKKFLDKYATNNLLNKNSVTNVRKKKELERKTIVNGFTAFRIYYSKFGKTYKDQENLSKELATFWKSDKSIQHTWHGYSEEYKASDTKLSFVKWFDTYKSAIKVQTPPEESLTQCTKISHLIVEDVYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLU0</accession>
    <name>A0A1E3NLU0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_107063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018217.1">
        <property type="nucleotide sequence ID" value="XM_019159709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLU0"/>
    <dbReference type="GeneID" id="30176396"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="103" mass="12562" checksum="B9DD9456C54BF347" modified="2017-01-18" version="1">MKRTRSYNEFYHDDHHGGQYALKFRIQKLVEEREAQILQLKEEYFQILDDLETFYADQSQQLCSWLWSHTQSRIEFISKKVEERIKEEHNCGGVRLEHKKVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLV2</accession>
    <name>A0A1E3NLV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 34 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_15114</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018230.1">
        <property type="nucleotide sequence ID" value="XM_019160674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLV2"/>
    <dbReference type="GeneID" id="30177361"/>
    <dbReference type="OrthoDB" id="205108at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF5">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="65783" checksum="ED29AE528C5DBEB1" modified="2017-01-18" version="1">MSSNYREDVDYEDTTGKDKSQRNRRLIGAAVLEYVKRPMTLPLFLVVVLGLLVFDVVPMGRGTVLLDPFLGGSIDPQTHDFFAPTQQNYNFYNRINQYLSPNHEPLGFQNIQFSRVRTTPGSPPVVANPGIYPFNNLDPADEQLKAITANYKAQNGFLKTLGYILTLGGRLSKNQLPKRKIYIILGSSPNIGIEREMSRQNWLVEKISTLNKKAYARRHNYELVFQNSANNIASTVRTDTLDSISGHQKRYQHESREGWERFDLMRQVMRTHSYNNPNVEEWYWYLDLHTMIMQPERSLEEVVFSVIEKASRSGAFLYHADNLDAIGDGDMTWSTLAHRREFGRLDLDPESSKLTHKDIDLILTEDCYGISLSSFLLRRSKWSELLLDMLWEPVMYRQMHTEWSKLERNDLPHKYGFLLNEGNENKESYRGAPSEMEERNCLEYFLSTQAWLRTRTAVLPARVFNSLSNDSCVIDTESTLDLIDMEMDDDLIDALLDSDDLDLLIIKEKLSANAMSSKFKELTSRVHDLHYDTSDFLFSFSACGGDCADRVKDAMAWYKKIHGKQIFEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLY0</accession>
    <name>A0A1E3NLY0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018252.1">
        <property type="nucleotide sequence ID" value="XM_019163872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLY0"/>
    <dbReference type="STRING" id="763406.A0A1E3NLY0"/>
    <dbReference type="GeneID" id="30180559"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000007">
        <property type="entry name" value="Mitochondrial tricarboxylate transport protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF4">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="17"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="115"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="214"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="302" mass="32214" checksum="2A948098EC0B381F" modified="2017-01-18" version="1">MSSNNGNGSVNAPPKKPDPFKSFIAGGLAGAVEGVATYPFEFAKTRLQLVDKSSKMSRNPLVLIYTVARTQGVSALYIGCPAFVVGNTAKASVRFLGFDAIKRVLSDKDGKLSGPRGVLAGLGAGLLESVVAVTPAEAIKTGKIDDKQSLKPRYQDGVRGTFRLIKDLGMKGLYAGLLPVAMRQGANSAVRLGSYSKIKGALQDWSDTPRDKPLSSGLTFLLGAFAGIVTVYTTMPIDTVKTRMQSLDASKQYTSTMNCFVKIFKDEGLMAFWKGATPRLGRLILSGGIVFTIYEKVLEVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM38</accession>
    <name>A0A1E3NM38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RPS0</name>
        <name evidence="11" type="ORF">PICMEDRAFT_49761</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with RPS21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018317.1">
        <property type="nucleotide sequence ID" value="XM_019163141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM38"/>
    <dbReference type="STRING" id="763406.A0A1E3NM38"/>
    <dbReference type="GeneID" id="30179828"/>
    <dbReference type="OrthoDB" id="414863at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000010">
        <property type="entry name" value="40S ribosomal protein S0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="208"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="265" mass="28908" checksum="EE480984759F1A16" modified="2017-01-18" version="1">MADFSLTQEDLQLLIAANAHLGSKNANVHQTGYVYKTRVDGVNVINVNKTWEKIVLAARIIAAVPNPADVVAISSRTLGQRAVLKFASHTGATAIAGRFTPGSFTNYITKSFKEPRLIIVTDPRTDAQSIKESSYVNIPVIALSDVDSPAEFVDVAIPCNNKGKHSIGLIWWLLAREVLRLRGTLPNRTEPWSIMPDLYFYRDPEEPTVEGAEENDEEAAEEDAEATEPKEEWAAEGDAQDWAAEGADTKDWAAEGAGDVAASAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM43</accession>
    <name>A0A1E3NM43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.256</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_71298</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc(2)Man(9)GlcNAc(2)-PP-Dol to produce Glc(3)Man(9)GlcNAc(2)-PP-Dol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = a alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29543"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19512"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="ChEBI" id="CHEBI:132523"/>
            <dbReference type="EC" id="2.4.1.256"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:29544"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ALG10 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.256" evidence="4 14"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018313.1">
        <property type="nucleotide sequence ID" value="XM_019163886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM43"/>
    <dbReference type="STRING" id="763406.A0A1E3NM43"/>
    <dbReference type="GeneID" id="30180573"/>
    <dbReference type="OrthoDB" id="4769at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106073">
        <property type="term" value="F:dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016900">
        <property type="entry name" value="Alg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989">
        <property type="entry name" value="ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989:SF10">
        <property type="entry name" value="DOL-P-GLC:GLC(2)MAN(9)GLCNAC(2)-PP-DOL ALPHA-1,2-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04922">
        <property type="entry name" value="DIE2_ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028810">
        <property type="entry name" value="Alpha1_2_glucosyltferase_Alg10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR028810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="474" mass="55171" checksum="AE84EAC571817ABB" modified="2017-01-18" version="1">MTLAESLLDVVVAGIATLPIKQFTEDVAMQANYQFIDEIFHYPQLKRIIRGDYTTWDPKITTPPGLYYLTALYSKVFKVECTLENMRYFNYFGGVFLVAMVILIRLRTKEPGFTSAALFMNPLLSIFYSLYYTDVWASAVVVAAYAVVVWKPFNNYFLTSVISALISLISLTFRQTNIIWSIFLLAALIDSKAKDENLYKYKEGVGDLLAFIKTGLKNFAICIPYIAVGLAFVGFVYTNGGIALGDKENHILSPHLAQLCYCMTFIALFTMPLWISYSVFIEYVEDNFLNLTGLFFNAAWLPILFIMIQSFTIIHPFVLADNRHYTFYIVRNFIIRTDTSRYELLPFYHFSFYIVYKLFKENSNILPDKNNNHRRTAASPIMYCAYMFCTAAAVTLSPLFEPRYYILPYIFFRLFTRPTDTPLLAVSLLKEHNTKIRYVGEVLWLWFWTQAIYLVFLQFTFKWDDIPELQRVIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMI9</accession>
    <name>A0A1E3NMI9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_23666</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017941.1">
        <property type="nucleotide sequence ID" value="XM_019162317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMI9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMI9"/>
    <dbReference type="GeneID" id="30179004"/>
    <dbReference type="OrthoDB" id="200187at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071256">
        <property type="term" value="C:translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011553">
        <property type="entry name" value="Sec62_asco"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00869">
        <property type="entry name" value="sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="159"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="231" mass="26493" checksum="B22E9AF3E058A675" modified="2017-01-18" version="1" fragment="single">AAIHIATAVRYNKLLKQRQGILTSSKNRTDFFRFKRFVRAIQSDEFKKSLAKGAKDLPPIPDVADAINQVFILLIQNQLVVPVTKLKTKDAKAKGLKVDKQTPALEMSNKAVLQPDVYYAWNYTPPNPYMLLYSILGICVVFTIILFPLWPLWMRKGVWYLSTGLLCFVGMFFVIAIIRLVIYLLTLASMSRQLWIFPNLFEDCGVLESFQPAYEWEDPKAKGKKPKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMM4</accession>
    <name>A0A1E3NMM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018482.1">
        <property type="nucleotide sequence ID" value="XM_019160774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMM4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMM4"/>
    <dbReference type="GeneID" id="30177461"/>
    <dbReference type="OrthoDB" id="1290869at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044289">
        <property type="term" value="C:mitochondrial inner-outer membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13971">
        <property type="entry name" value="ADCK2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044095">
        <property type="entry name" value="ADCK2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052402">
        <property type="entry name" value="ADCK_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890:SF1">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2 (PREDICTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="275"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="719" mass="82306" checksum="EBDCDF707620CF5A" modified="2017-01-18" version="1">MAYSNLRLLSRSFRPCGLRGRENFLCLQNSSSIRPVLPRPLSKATVPSYTLRSTYSTNSYKHGSHKSRSSFSGILIGSVCLAGCGLYLSSSHYSLIDIVHNDEQKKKSKVSKAQQVEGAQPFVENNWQDSPDSQPHNSLKPDPSQDTFEMGLYAASRAEQEELEYQYRKERTDRKFTGWLFHVGFFFQDYVYEPIVTFGRFLELSVIFVPVFALYPITFFGKTDKSGERSGARLWYWLLRNAAEMGGASFIKLGQWAASRTDIFTKGLCTEMSNLHSNAKEHSFRATRRILRETFNGKELEEIFDEFPEKPVGCGAIAQVYLAKLNQQFVGSGDKHDTLVAVKVVHPRVTKNIYRDLKIMSFFANLIDVIPTMEWLSLPDEVEQFSILMNLQLDLRIESYNLNKFREKFAKANDVFFPKPRYSSRKVLIEDQVYGISMNKILELKHAKNDKMLKFISDRLIDSFLKMMILDNFTHADLHPGNIFVRFVKSKGYQNSSSESSKFEIDKLTTKLNGLSVDELNQELNNMVTEKYVPQLCFIDAGLVTELNDQNRFNFISLFNALAQFDGYKAGELMIERSKTPETAVNKELFKLKVERLVNKVKERTFTLGSVSIGDLLDNMLGMVRSHHVRMEGDFVSIVVAILILEGICRQLDPEIDLFARSIPILRQISFQEGKLIFQNTNKLSMLKVWLTLEVRQFVNASVHDVHKLVKSDGLAPNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMV6</accession>
    <name>A0A1E3NMV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_31777</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018562.1">
        <property type="nucleotide sequence ID" value="XM_019162720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMV6"/>
    <dbReference type="STRING" id="763406.A0A1E3NMV6"/>
    <dbReference type="GeneID" id="30179407"/>
    <dbReference type="OrthoDB" id="1109245at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="8">
        <location>
            <begin position="37"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="257" mass="28863" checksum="E0191246A4EC6F15" modified="2017-01-18" version="1" fragment="single">KHHLKRLAAPSHWMLDKLSGVYAPRPSTGPHKLRESLPLIVFIRNRLKYALNGREVKAILMQRQVKVDGKVRTDTTYPAGFMDVISLDATNENFRLIYDIKGRFAIHRITPEEAAYKLCKVKKVQLGKKGIPYVVTHDGRTVRYPDPAIKVGDSVKVELETGKISDFIPFETGKLAMVTGGRSLGRVGVITHRERHEGGFDLVHLKDSLDNSFITRLSNVFVIGNEAGKPYISLPKGKGIKLSIAEERDRRRAMQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMW7</accession>
    <name>A0A1E3NMW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_71508</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Lipid-anchor</topology>
            <topology evidence="1 10">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018549.1">
        <property type="nucleotide sequence ID" value="XM_019163940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMW7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMW7"/>
    <dbReference type="GeneID" id="30180627"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000005">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF2">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027320" description="1,3-beta-glucanosyltransferase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="542"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="377"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="471"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="561" mass="59945" checksum="A6F54AFFF59B34C9" modified="2017-01-18" version="1">MKFTSTLTAAISSLLFAGISHAADFPTVEVVGNKFFYSNNGSQFYIRGVAYQQNTANLSSDASYVDPLIDEDACKRDIPYLTQLNTNVLRIYALNATGNHDACMEALQDAGIYVIADLSAPKESIITDNPEWTTELYERYTSVIDMMQGYDNVLGFFAGNEVVTNSTNSDSAPFVKAAIRDMKAYISDKGYRDIPVGYSANDDSHTRASSADFFACGENDVKADFYGINMYEWCGDASFSSSGYEDRTKEFSNLTIPIFFSEYGCNSEHPRKFTEVGTLFSDKMTDVWSGGIVYMYFEEENNYGLISLKGNSVSTLGDFTNLKSEMNAIHPSSATVSQASASAHQLKCPVTGGNWIAATALPPTPEQDVCDCISKASLCVVDDDVDDDDYADLFGTVCNYISCDDINADGSEGDYGPYSFCSSKEKLNYVLNKYYEDQGKKSSACDFSGSATVVSASSASTCSSVLKNASASGGASGGGKSKQATATSDGQTGKSTKTAKKDDSSKDESSSQTSAVSSISSSSKAGASGLETPFRSLGLSSAYIVTLVASVTLGGITMILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN34</accession>
    <name>A0A1E3NN34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017946.1">
        <property type="nucleotide sequence ID" value="XM_019164314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN34"/>
    <dbReference type="GeneID" id="30181001"/>
    <dbReference type="OrthoDB" id="4083086at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="263" mass="30445" checksum="91C77079D7DF43A6" modified="2017-01-18" version="1">MADPKDETRKGVPDLDRRPNPPGWVQPKAPYNPYDPTDLRPAEGYPSEYNAPSAMKRNTGQLARDLTDYQKIKIDMKKLQYYPRPPSELYPGRYKVLRRVNHKSGFTRGADLTAKVSIYGVLIFGVFFYRWNDHDNIFSPFRRLQLRAKELLLGELSVDDYNDLYHYDADLPIPQKPLPSRMYEKNAEQNLVGESATNNEFIKDRFGNKHVVYAEHIKQKEDENMVKALEIAQREIELKQLGSSRAGGDPGAGQQKKPFWKVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN38</accession>
    <name>A0A1E3NN38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">homoisocitrate dehydrogenase</fullName>
            <ecNumber evidence="12">1.1.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_71583</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R,3S)-homoisocitrate + NAD(+) = 2-oxoadipate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:11900"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.87" evidence="12"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018633.1">
        <property type="nucleotide sequence ID" value="XM_019163958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN38"/>
    <dbReference type="STRING" id="763406.A0A1E3NN38"/>
    <dbReference type="GeneID" id="30180645"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047046">
        <property type="term" value="F:homoisocitrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000012">
        <property type="entry name" value="Homoisocitrate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF48">
        <property type="entry name" value="HOMOISOCITRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="13">
        <location>
            <begin position="21"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="372" mass="40174" checksum="03C510B4A6A81573" modified="2017-01-18" version="1">MLAASRNTARRAYSTAPKSLTIGLIPGDGIGREVIPAGKQVLEALPSQFGLKFNFVDLNAGFELFKKTGTALPEDTVEALKSSCDGALFGAVSSPTIKVAGYSSPIVALRKKMGLYANVRPVKSVEGIGRPVDMVIVRENTEDLYIKEERIFEKDGQKVAEAIKRISESATRRISKMAYEIALQRQAVRDLGADSLHTKPSVTVVHKSNVLSVSDGLFRETCKAVYEENIEKYGSIDYKEQIVDSMVYRMFREPEIFDVVVAPNLYGDILSDGAAALVGSLGVVPSANVGDNFAIGEPCHGSAPDIEGRGIANPIATIRSTALMLEFMGYSEPAAKIYSAVDANLKEDSIKTPDLGGKSSTQEVIDDIVKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN49</accession>
    <name>A0A1E3NN49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bola-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71592</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018644.1">
        <property type="nucleotide sequence ID" value="XM_019163960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN49"/>
    <dbReference type="STRING" id="763406.A0A1E3NN49"/>
    <dbReference type="GeneID" id="30180647"/>
    <dbReference type="OrthoDB" id="203381at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12168" checksum="53D897D2B1B7CDA1" modified="2017-01-18" version="1">MFPSSSKRIASLIPTLRALPLRRFYSAPPSLDAAEKHIYEKLEKELEPLMLDVRDVSGGCGSMYAINIVSDKFNGISMVKQHRLVNSILKEEISGWHGLQLKTRANKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN57</accession>
    <name>A0A1E3NN57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018657.1">
        <property type="nucleotide sequence ID" value="XM_019160841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN57"/>
    <dbReference type="STRING" id="763406.A0A1E3NN57"/>
    <dbReference type="GeneID" id="30177528"/>
    <dbReference type="OrthoDB" id="5331396at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37849">
        <property type="entry name" value="YALI0E11605P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37849:SF1">
        <property type="entry name" value="YALI0E11605P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="92" mass="10344" checksum="FC8B3DD728C10AAF" modified="2017-01-18" version="1">MMFAQLTRKSVQTIASRRFASTLVSPRPSSLKGVLFGFFAGVSITGFGCYYYLLDEYKASSDAIISDILLLQKTIRNMETHIRVLESSIEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNG9</accession>
    <name>A0A1E3NNG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription elongation factor SPT4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Chromatin elongation factor SPT4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15178</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018301.1">
        <property type="nucleotide sequence ID" value="XM_019160701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNG9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNG9"/>
    <dbReference type="GeneID" id="30177388"/>
    <dbReference type="OrthoDB" id="248751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001208">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="9">
        <location>
            <begin position="35"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="139" mass="15760" checksum="6E1FC0A8A924C471" modified="2017-01-18" version="1">MRHPVHSSGQTRTQIYTSAEDMSTLKRGRQTPKLNRACMLCGMVQSMSQFRNEGCPNCESVLHFQDNEDVIRECTSPTFEGLVAVRGSSSSWVARWLRIENFVPGFYAVRIVGKLPDDIKNDLYAQGIEYRPRDGSVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNS1</accession>
    <name>A0A1E3NNS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018441.1">
        <property type="nucleotide sequence ID" value="XM_019160756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNS1"/>
    <dbReference type="GeneID" id="30177443"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="186" mass="20603" checksum="9B9006D482205600" modified="2017-01-18" version="1">MFETVSATGEREKRPGRKRVNLKQQRREGEMKKNEEKFRKTSQSQRKFNASTPSISLTHSSPTLLLSLSLPLLLLLPQPALLALAIFAIFAIFAARRRRFFSKHVNRSATLAAPGGGPRELQPVYVGVAKRASACCTTRLCFLFRPPHTSVCAAALRVRSRACCPAPPLTLSANPLRRPLWLRAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNU8</accession>
    <name>A0A1E3NNU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS28 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_24729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018870.1">
        <property type="nucleotide sequence ID" value="XM_019162364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNU8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNU8"/>
    <dbReference type="GeneID" id="30179051"/>
    <dbReference type="OrthoDB" id="2671at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007143">
        <property type="entry name" value="Vps28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017899">
        <property type="entry name" value="VPS28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037206">
        <property type="entry name" value="VPS28_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03997">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140427">
        <property type="entry name" value="VPS28 C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51310">
        <property type="entry name" value="VPS28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS28 C-terminal" evidence="2">
        <location>
            <begin position="9"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="101" mass="11129" checksum="9E35DC20DFF7996B" modified="2017-01-18" version="1" fragment="single">ATAPAGDTKGKKAIAEATSAFITLMDAIKLNYDSKDTLHPLFSDVLTKSGQVSTDFDGRNKLVSWLIRVNKMGIADTLDENVLKEMLWDVDTAYNGFFNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP02</accession>
    <name>A0A1E3NP02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 7">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_10404</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a central role as a glucosyl donor in cellular metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 7"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018521.1">
        <property type="nucleotide sequence ID" value="XM_019159664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP02"/>
    <dbReference type="STRING" id="763406.A0A1E3NP02"/>
    <dbReference type="GeneID" id="30176351"/>
    <dbReference type="OrthoDB" id="932129at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="499" mass="55738" checksum="BAD056DE30E3A8C8" modified="2017-01-18" version="1">MSKHLKSQSTFAFENTSSNVAASQMRNALNALVDSLPAELNSQEKLRFENEMDSFFALFRRYLADKATSSKTLDWDKISSPTNGKVESYASLNDDSSNKNFDKLAVLKLNGGLGTSMGCVGPKSVIEVREGQSFLDLSVRQIEFLNKTFDTDVPLLLMNSFNTDRDTELIVKKYQSHRIRIKTFNQSRYPRIFKDNLLPVPKHFDDQLDAWYPPGHGDLFESLVSSGVLDSLLKEGKEILFVSNGDNLGATVDSKILNHMIETGSECIMELTDKTRADVKGGTLIDYDGQIRLLEIAQVPKDHVEEFKSIKKFKNFNTNNLWIDLRAIKRLVESNSLHMEIIPNQKSITIGNSEINVIQLETAVGAAIRYFNNAHGVVVPRSRFLPVKTCSDLLLVKSDLFNLQHGTLVMNPARFGGAPLIKLGSHFKKVSNFQQRISHMPKIVELDHLTVTGNVFLGKGVQLKGTVIIVCSDGQRIDIPNGSILENVVITGNLQILEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP89</accession>
    <name>A0A1E3NP89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_121151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019027.1">
        <property type="nucleotide sequence ID" value="XM_019159883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP89"/>
    <dbReference type="GeneID" id="30176570"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="76" mass="8404" checksum="DBBC2B948789CE18" modified="2017-01-18" version="1">MSGAPRRLYTFVFFPLGRSSLFVYLLPYTEFFSCRCTPSKCQIRRPEARRGTPTALSKAVISRTQTGAAGVLLGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPI1</accession>
    <name>A0A1E3NPI1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_26660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019143.1">
        <property type="nucleotide sequence ID" value="XM_019162457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPI1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPI1"/>
    <dbReference type="GeneID" id="30179144"/>
    <dbReference type="OrthoDB" id="9984024at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053169">
        <property type="entry name" value="MUG_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 191 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791:SF3">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 191 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="300" mass="34724" checksum="90FA5C78B84FD703" modified="2017-01-18" version="1" fragment="single">MSFDNNLEGFNTCQKMWYFFWNPSVGSFTTQVPCHESHGNQFSCYAQAVAMHAIADSASVNKDMTIPIVDKAIKSTLKYRNPKYGAYSVDFHGGINSGDEDINYDDNAHLLRALIELYEATQNRNYMNMCKELMKFLYTGIIEHQIWHVPGLKWHITKPYMNTISNSVSAIGAMRMIPYAENQQEEQKLYEFAKICINFVWNKLLDPSDDIIMDGVGWDSENLDITKYSYNQGTTLSAICLLYKYDHNPEWKEKAIRLVDGCINPSKTLFDRDYKEYDKRFLQGVSYFNQLLIEGVVDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPR1</accession>
    <name>A0A1E3NPR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA N(3)-methylcytidine methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019158.1">
        <property type="nucleotide sequence ID" value="XM_019164083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPR1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPR1"/>
    <dbReference type="GeneID" id="30180770"/>
    <dbReference type="OrthoDB" id="417697at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052735">
        <property type="term" value="F:tRNA (cytidine-3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106217">
        <property type="term" value="P:tRNA C3-cytosine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000221">
        <property type="entry name" value="Methyltransferase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026113">
        <property type="entry name" value="METTL2/6/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809:SF11">
        <property type="entry name" value="TRNA N(3)-METHYLCYTIDINE METHYLTRANSFERASE METTL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037755">
        <property type="entry name" value="Mettl2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 12" evidence="5">
        <location>
            <begin position="128"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="37893" checksum="34F8B7EC11A628F7" modified="2017-01-18" version="1">MSENHVAAELVASAEKPPLSSRIGRDPPFVFGQRFLTEDADVFNHNAWDNVDWDERQIEQFQESILKQKESPVSDFNKTEYNSKPKKYWDLFYKNNRENFFKDRKWLQIEFPSIFECTKKESGRKTILEIGCGAGNTMYPILSKNENPNLRVFGCDYSDIAVDLVRGNENFERLHDSGNCYSSVWDLANEEGNIPEDLEDNGVDIAVMVFVFSALSPKQWEQAISNLKKVMKPGGLILFRDYGRYDLAQIRFKKNRLLDENFYVRGDGTRVYFFTEEELREIFCTRGGFIEEKIGSDKRLLVNRKKQLKMYRCWLQAVFKVPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ41</accession>
    <name>A0A1E3NQ41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_58479</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019314.1">
        <property type="nucleotide sequence ID" value="XM_019163302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ41"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ41"/>
    <dbReference type="GeneID" id="30179989"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133460" description="Thioredoxin domain-containing protein" evidence="4">
        <location>
            <begin position="27"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="21"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="739" mass="85551" checksum="C276A4CD8229577A" modified="2017-01-18" version="1">MPQMKHILLVCWILLGFNIGISQAVAKPSTGEDDLHSRVPPPLTATDFDKVLEEGFHVVEFFSPYCPHCTQLFPTWVEFYEYNQNMNGKNSKDKSNSFQIHQVDCVASGDLCEREGIQFYPMIRFYGAGSKLLGSMTTNPKDLDTMNEFANEQIIVWSESEDDDDMDASALIPGTPLYNQNKMIKAGELLKIIEGENTVPKLISFWPTSESELNNQNFQNDYNQNKIFSYYPNLHPFRNLWNLSMKKLKNFINEDKLEFNFFNCKSNPEICETLGFKSLTESRISPNLSPQVILYLPKTTGGNAISYKLKINEFHNLNTAVKSLTHWTQRTLINSELEDMKFQDIQDFIGSSTKLSRKGSIGEITDYSKVAFVQVNDPESEVPEDDAILSRLIQPIADLKSDVYLFRTTDKDAVMKFLRDQEEQMANKYIHLGVQDTAMNFDEKMFLSRTHTTFPMMICIKSGSLYSPVFTSFMSKHIRDYNKVLTFIKRNYMPTINHLTIDSKDQIFPPTLNERINSRTEKVLITLTDFQPKQYFDVEFFMSFVFHKFTFLNNRDKFNRIDEKRSMKYDKVGELRANKADSDDIIDALREKVDISYTTTDNQLYPVYLDTKKLPEIISAMKWSKLDSSRYTTGDVILIDRFNGRYWDEDDSGHKLTIEDPQQTVGLLEKVSYKKLKGKSLKHLSLFAKVPLYSFIAILIFMTYRAVRVYLQKRTVYIEKMKGLGILGVCPELEESKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ84</accession>
    <name>A0A1E3NQ84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019354.1">
        <property type="nucleotide sequence ID" value="XM_019159762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ84"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ84"/>
    <dbReference type="GeneID" id="30176449"/>
    <dbReference type="OrthoDB" id="20109at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013241">
        <property type="entry name" value="RNase_P_Pop3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28272">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28272:SF1">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08228">
        <property type="entry name" value="RNase_P_pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="400" mass="44921" checksum="172174D4477E1E02" modified="2017-01-18" version="1">MAITLSKEELDAHPGTKSMCQYGAIYTNGAIVCQHKHVHSPSIVGIMFALAVATMVIAMIVKWVRKQDQSGQHHHYYPHSSAFSIFQNHDSDVEQLNPVPVYTASADPSNDFGFYDPNGNLVALQKPLLTYQRDTPPTSQPPEYAPAVPITNIQPHITPVLDYVEYKQETDTDADTDTDTETDSAPPLPQQPPKYSKALTSLKAQEVQKKSVFKPLLTNPYTRKNAWPKIDLKLQTDLLQALETTILPPVKQYNQLTQEEKKVFSESNNYESDVLTGFNSIMKALEAQVQSKLKKKIENESNDITMLFVSKVKLVTLPKGSSKRLSSAVGKKNELQFMALRRRFIEKDAFISSVVNSSVDDVSVGFLDNIERQQLNMNVKFVLTEIPIVGRTRKPDKQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQG5</accession>
    <name>A0A1E3NQG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_13938</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019462.1">
        <property type="nucleotide sequence ID" value="XM_019160143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQG5"/>
    <dbReference type="GeneID" id="30176830"/>
    <dbReference type="OrthoDB" id="3986994at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051430">
        <property type="entry name" value="Fungal_TF_Env_Response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944:SF131">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="824" mass="96462" checksum="B24264DF27A0D3BB" modified="2017-01-18" version="1">MSEISKVKRRRNRVSLVCSNCKEKKIKCNRQQPCSNCIKSSLISSCTYNYSFKKEKDSSLTTEEKNEEKSKETEVVSADDVHWEEGSPGTTYQKTILDVEFSYPNEYNVFRKPSNTQLRPIFHCAYMREEHVLSFKFLMSFKQLLNNERRLWKSKNFNKNFQLIELQYGGIDIKDGSACQILNQWVNDMICNNYYAILERLRYFQTELNKILFNSYIPMGVVQLIFHYYFNMKQEGVVFRRPRKNFEYSFIALITSLVELTNIFTRYADYSFNFPLPQQNNEFNELSVKLLNASNYRRKRTIFAVYTLLNLRLSLMVYGDAQSGGVAMQNTQPLFQTAVSIFIEMGLHANLDKILYFEPPPNVSNGHEHFNELFFAKDIPTESLKVLWNYLLVTDITYYIDLSTHPFINDRFCHGFYKLANRESTSTESIWNLIREISYELLSQKPYPLRMIAKYDSKLIKLLSSLGSFADYQSFEKNEEKWQSFHLMFKILKLIYMFQFKIHAFLDEGNLYENYSKDVVDDKKNQEIIKSLKKECGIKSKIVYFLGISALLKMSESNLSYKFLLYNREVFSTWIGTESILLIDMILTDGLRAKNKKIVPAMDEPKNFSLPEVRVFDIKELEDALYNFSAKKHSQLLLQIEDTCEPENILSFMSYCYEKIINVPALFSDYKFFVMTKLFFLTIYFLYSYIETHCDSFKTVYNNLEKLRAMTQKIISKNLQEGRLLHLLPTHQVIDELRERDDGSKNAQPKSDSLSNLSLSTNTISKLGEQMHSSADINWTPDSLSTTSSMLPSQFDDIASAVFEDEGMVNILNEINQYFNQPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQW4</accession>
    <name>A0A1E3NQW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminopeptidase P N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019594.1">
        <property type="nucleotide sequence ID" value="XM_019163558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQW4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQW4"/>
    <dbReference type="GeneID" id="30180245"/>
    <dbReference type="OrthoDB" id="10261878at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000002">
        <property type="entry name" value="Xaa-Pro aminopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF1">
        <property type="entry name" value="XAA-PRO DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="465" mass="52917" checksum="F6AAF8C3D59A3585" modified="2017-01-18" version="1">MKYPAKAHALKVKNHFLAEKKSEKKDGIAFFIASATYTNYPYCDQVAPLRQNRYFHYLSGIDQMAGAFILFNVLNEKLTLFLPNIDDEDIMWSGLPLSPEDALKEYDVDEVLYAKDVTKTLESLKSSNFDIYTTDTDQYSKAAFAKLVIPKDTSFFYALDESRLIKDEFEISLMRHAAEITDNCHMAVMSALPIETNETHLHAEFVYHSLRQGSKFQSYDPICCSGPSCGTLHYVKNDDQMDGKTSVLIDAGAEWKCYASDVTRCFPITGKFSKEHLDVYETVRDMQAQAMKEIKPGVAWEDLHLLTHRVLIKHFLELGLFHNGTEDEIFNSKVSAWFYPHGLGHLLGMDTHDVGGNANYEDENIMLRNLRLRRKLQAGMVITDEPGVYFSPFLLEHISEEQEKFVNREIVHKYMYIGGVRIEDDILVTADGYENLTGITSDPHEIEKIVQKGLSKGKGGFHVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQW7</accession>
    <name>A0A1E3NQW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">6-phosphogluconolactonase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_51054</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in regulation of tRNA subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019086.1">
        <property type="nucleotide sequence ID" value="XM_019163151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQW7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQW7"/>
    <dbReference type="GeneID" id="30179838"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000017">
        <property type="entry name" value="6-phosphogluconolactonase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="8">
        <location>
            <begin position="80"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="38"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="313" mass="34328" checksum="5A093BD5F830ECCA" modified="2017-01-18" version="1">MTTAVPLIYSFPEFSGVAEAVADLVVTAQNKALSKSHDDIAPLNTTSDSPLMLRNTPDDASIHSNSMSTSPSTSSQLRKKKKESNRRFKIGISGGSLLNVLHEGLLKRDDIKWDKWDVYFADERLVPFDSSESNYGQAKSKLFDLIPESKGSPNIYPINSALLDDPQECADDYEKTLIKGFASKDSVKLPMFDLILLGCAPDGHVASLFPNHETLRENYAWCVPVENSPVGPANRISFSVPVICHSHLVAFVVEGSTKAPVLRTIMERPDKGLPASIINEKAAGKIAWFVDDDAISDVVGIIKKRYKFTATTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR11</accession>
    <name>A0A1E3NR11_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019638.1">
        <property type="nucleotide sequence ID" value="XM_019163572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR11"/>
    <dbReference type="GeneID" id="30180259"/>
    <dbReference type="OrthoDB" id="5330253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018809">
        <property type="entry name" value="DUF2406"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186:SF1">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10295">
        <property type="entry name" value="DUF2406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="195" mass="22190" checksum="D2F62AFF63E9F30A" modified="2017-01-18" version="1">MGIFSKKNKDGEDGHKRSNSKGNDLKITDEDERRMKTRSQNLHDPILDAVNEAQPFEEANMHNRSSSIGNGVLNDIFGNKIVNPDISNPTRSRDERPMDTIRSFEYSITGDPIYHEQLETHILGWRVRDDFPLGNGYAANNQVQYDEYGQPVYTNTRANQPIVEQGVYSAPAPKIEEQKKKKFFSFGKKKKEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR41</accession>
    <name>A0A1E3NR41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_70164</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019652.1">
        <property type="nucleotide sequence ID" value="XM_019163577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR41"/>
    <dbReference type="STRING" id="763406.A0A1E3NR41"/>
    <dbReference type="GeneID" id="30180264"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000010">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000011">
        <property type="entry name" value="Sulfite reductase flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF109">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] FLAVOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="14">
        <location>
            <begin position="655"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1042" mass="116615" checksum="50D7A2527E603C50" modified="2017-01-18" version="1">MSQAGVSTISELFSATAVAPFGLPLDPYEVKSRTLSTKYNIIQGALYLLSSNIFNYALPTVSESDENLIQLWSNLKRKNFSNEIPKLHNLDISTDSGNLLLGYLKNFKGSATLNASVDALLNFAPVLVSHSNNEYHSLPLSIQLSTVSYNFEKNQLVSTYSEALSIAKSLRLPVLTSTTALEAQLFTLVSIVLSKFNIANIHLFNGLESIRSNESLSGLLSSSELKHLSDKLYNSLKADILSVNPTEIVSLALNKLNEIQGSDYQVFKFYGNKKAEVVYAIYPTKESISALVESVGTESEFGFILVKSPIPFASKQFNSLVSADVKKIVVVSEFLNDSTSSLKLDIQASLFFESRFHTQVVNDTFDFVVKKFASSFSSASSAASYKFLLNDNSKFIELPAKLAHALSLTQELDVKFRPVYDNTVNAGLFQADIQTGSVYDYSGKFDFIVVEDTNILNSVDVFSLLKSGGKLLVINNEETVFDESKIIEKSLSVKVKKILAQSKSNLDFLDLNVIGEVDGTKGRTASIAIQTAFWSLNYPSWDKHSVVTKILQFFGSEAELLASVIADQVENVKANGFKEIKFDVEWEKLEDAEIEGSEEGDVFAIVRETSFKPNPREAFIDNMTVKTERKTDLTKKLMFKEAYGTTSSLRPDQPVENFVVKVKENKRLTPGDYDRNIFHIEFDVSGTGLTYNIGEALGIHGRNNADLVNEFIQMYGLDGDELIEVVSKDSHEVLEVRTLRHSLIENLDLFGKPPKRFYEALVEHATDETEKKKLEQLISPAGGALFKKFQEEEFYTFADIFELFKSVRPAAQELVQIISPLKRREYSIASSQKLHPTEVHLLIVVVDWVDKKGRKRYGQCSKYISDLRVGDELVVSVKPSIMKLPPLTTQPIVMAGLGTGLAPFKAFIEERQYQLEHGAEIGEIYLYLGSRHKRQEYLYGEYWEAYMDADVLTHLGAAFSRDQVKKVYIQDKIRENLDELTDLIINKNGHFYLCGPTWPVPDISACLQDMFAADAAHRGVKIDAAKAVEDLKEEGKYVLEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRF0</accession>
    <name>A0A1E3NRF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_85366</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019773.1">
        <property type="nucleotide sequence ID" value="XM_019165014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRF0"/>
    <dbReference type="GeneID" id="30181701"/>
    <dbReference type="OrthoDB" id="5426978at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF26">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="191"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="412"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="451"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="964" mass="106811" checksum="BF795FFA54538E33" modified="2017-01-18" version="1">MSDNQSLSGQAVPKAAGLASGPDANAGILEKNPTDITSILNPSRSTAHLTTLGDSLSPGTKPADLQPLHESSNEAEDAADNDNDDGDAAEDDNDNDNDNDHDNDHDNDNDEEDEDEDDNGRRNGQEENDLMQTGKKHHARLPPRKRSKVSRACDECRRKKVRCNAVFDMNSNSIVKICTNCEKNNDSCMFTRVPLKRGPNKGYTKKSSSSSSINSSHTKPKNSKRLSLDSPEMPPSPTFFNPSGHPDTEMLRIDSNASIRSNSNRNSTLSQFVPPPPPHPASSVQPPYNNQQVILPPLNSIPFSDKLSDPNNQQAIFWKVPTEMPSLPPNLGSHARKGSVDSTHSSLSSASSISSVTRNSLLFSTGLNREAKSLVNAGVENSDSEDEFLATTSGRLSRSTFQVPFTLQSRSPRVSFTSDKEQRSSASSIMAPNSISSPTARVASPRFESIQPRSSSQNNPNFRSTEYKDSLYSLLDNYYANLYPQYPLLPNPEIMKMSIGSIIDNSDFFTIIELFSASLHFINVITSSTNSTPVQTYSSSPSEPPSSMAVRFREITRAFEVIASMYLSKSLIYQSVPGKILFTSTLLLLNYAVVLSGYDYSLGFGIAFSYFNDWLIFKEGYDSPCFANLIQLVVLDALHTLYYGLPRSSTVCFAINTDFIKILLQEISFTPGVDLEWLSVGLHLVVLSNNLQELDSLNKLETIVITGTEYKFMSIIKLYYELYVYCSHLNAELQRIISAHNEQSDSDVSLTDDFKRFIYNVELNLSKLCKKLTNLIDEQLDDIEMTKPNPMVVVVLVRCTHISINTQIFVRSIIHLNEVLDFSHSKSNFNFSSNAQARRHENRSGSDSSINSFGSLSGSGGNNSITDFSSVIGDCNRRFAKLNENVASNLNRSQNIKQTHQHARDIVNMLINSKKPEIVVPRTTLHSSKGGVDYSVVIQNWVRLVNIFFSGEITKEGINGWSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRK0</accession>
    <name>A0A1E3NRK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation-transporting ATPase</fullName>
            <ecNumber evidence="13">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_29686</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="13"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019825.1">
        <property type="nucleotide sequence ID" value="XM_019162604.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRK0"/>
    <dbReference type="GeneID" id="30179291"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903135">
        <property type="term" value="F:cupric ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07542">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000032">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000119">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047821">
        <property type="entry name" value="P5B-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="622"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="658"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1166"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1196"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1234"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1283"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1312"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1343"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="232"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="474"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ48712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1404" mass="159316" checksum="8BD6913C9F3725DD" modified="2017-01-18" version="1">MTSHMDVNDTEMFSGAASNAIPTSISSFHHHHHFRSPTLSVTSSHDDQNIDRIVLDRSESEASEFQERSKFRFFKSADIEAAKGVSSLGRFSSDNNHESVKIDYDTNWNSFADDYSTDHLNDTELERTAFYNSHKKLTSDDGSNSNNSYGSIGENPYSEFFRGDAERELVADEEQDLHIYGEQEGDETFEDMITKKAWLKTMKQRESLKMHDKFFKKFPAKNTSQRFYIAEEDLVAGIAGYQTTYWKLAIYNIICILTLGLAALICRWVPRWRIKMMGKPAPLGKAEWLVVEDEYGSMEIPKVSRKWYNRNLSTFLPPKKGSIMTGISHAYTIDDIDPIVPILISFEYRYMTLFYDPLDDLFKLSNNWIDEKWCHYPDIKDGITESNYRCRKMIFGDNIIEIKEKSVLKLLVDEVLHPFYVFQVFSIFLWLADSYYYYAFCIFLISVISITESLIETESTLERMRKMSRFECDVRVWRSGFWKEINSDDLVPGDIYEVSDPSLSTYPCDSILLTGDCIVNEAMLTGESVPVSKLPISPECVKELEMEFNGAKFSNRFSKSFLYNGTKIVRVRYGLSEGSGGKEPATALVVRTGFNTTKGALVRSMLLPKPTGFKFYEDSFKYIGVMSMIAGVGFIFSTINFLKLGLPWKVIVLRALDLITIVVPPALPATLTIGTNFALSRLRQKSIFCIAPMRVNIGGKLDVLCFDKTGTLTEEGLDVMGIHISNPIRDRQEHEFSEMLPSICDILESDIPNSEKIFTAMLTCHSLKVINDEYVGDPLDENMFQFVGWDMIEDPHESSILSDFIESKVLLTSSSPILFKKDGVPSHWIQLKEFEFISQLRRMSTICINFSNNYSQSPQEIEVYVKGAPEVIETICLKESIPANYNELLYHYTHNGYRIIACASKKLKNPSKNLNDLSFIGKLKRESIECDLEFIGFIVFENKLKSSTTDTIEQLSDADLRTIMCTGDNVLTAISVGKECRIVGEGAKVFIPSFDYDNFPKQSDNDEHAGIDRRISSNGIPPIIWTEADNPELMLDRITLAPLDINQDNNYCLAVTGDIFKFILTELAHMEFYIETILMKSSIFARMSPDEKHELVSQLQKLDYTVGFCGDGANDCGALKAADIGISLSEAEASVAAPFTSRIFEISCVLDVIKEGRASLVTSFSCFQYMSLYSAIQFISVSILYKEGSNLGDFQFLYIDLALIIPIAVFMSWSKSYSVIVKKKPTANLISPKIIIPLLGNISILLAGQLLVWFIIRHKNFPWYIKPIPGSDDQVQSTDNTTLFFYSNFQYILIALMLTVGPPYREPANKNVPFILTIAVTLSFSMILMLVNPASSFGQLMDLTWMSSGSKLGLTIGAFVNYFILEVGDKYVFLQLAQVFKRLVKRKGSNKKFKKLKREFRKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS73</accession>
    <name>A0A1E3NS73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_27694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020054.1">
        <property type="nucleotide sequence ID" value="XM_019162508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS73"/>
    <dbReference type="STRING" id="763406.A0A1E3NS73"/>
    <dbReference type="GeneID" id="30179195"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF260">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="417" mass="46929" checksum="6AD3FDCEB3A28856" modified="2017-01-18" version="1" fragment="single">PYPNPTKSFWIEEGPHTLKEHRTTDELPTEIDVVIIGSGYSGTSVACGLLLSKTSLFKKRPSVVMLEARDVCSGATGRNGGHIRSFYHSGHKHFVDQVGEKKAAEMVMFEHDELSKVEKLVKKYKIDCNFDPRLSCQTVDDPRVYQSSLDNYYAFQNNKYIPQSIKDCVKIRFVPEADEVSEHKAGPFCITAPTCSIWPYKLITSLLRRCIDTGLNLQTYTMVENLKPTCDGGWIVSTSRGPIKCKKVVCATNAWTRALLPEFKDKIVPVKGVVSHIKPISKNPGKLSFNYYHTFPIESDYVTAHTDMSMIAGGGGPTYLTYPNSADMFNNCDDTYAPEDTLDYFKDYPNKYYPEFAKGLKFVNDYTWTGCMAYSNDEFPFVGDMSPFGRKNLYIIAGFTGHGMPRIWSCGEYVSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSE9</accession>
    <name>A0A1E3NSE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="4">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14064</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019616.1">
        <property type="nucleotide sequence ID" value="XM_019160205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSE9"/>
    <dbReference type="GeneID" id="30176892"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044182">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140504">
        <property type="term" value="P:microlipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042998">
        <property type="term" value="P:positive regulation of Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050714">
        <property type="term" value="P:positive regulation of protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044011">
        <property type="term" value="P:single-species biofilm formation on inanimate substrate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05168">
        <property type="entry name" value="PI4Kc_III_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000021">
        <property type="entry name" value="Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021601">
        <property type="entry name" value="Phosphatidylino_kinase_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057754">
        <property type="entry name" value="PI4-kinase_beta/PIK1_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11522">
        <property type="entry name" value="Pik1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="10">
        <location>
            <begin position="768"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1066" mass="120196" checksum="5BC99036831A9E60" modified="2017-01-18" version="1">MAEQIKNVSPLEEEKVGHTNNVLSPDENKDVADSQEKPESADNSFSVSAPDSSANGLENHFEKLSIPVTKYQNGHRLENESDTTDLYTKINSKDFEIFHCISLLEHNDNIGIQYYICKRIQKFPIGELRFFAPQFLQLLITMETQSMALEEMIKVLCFRDPHFGLIIFWHLQAMLQDLSKEPESVGFQTCKRLVNELQYHLFNYSTNSKTSNSSFVSSSGVVPRNDLNKDFKPNIYPSIIFATSLLSGMTVPESTAYVEPIVRMEGKKVKTMVFEVVKDIKKSLTENLTKKNTLNNALMGKQRVYTDDLKRSKSINVLKERKTSSPNLKARGSSEYVKTRKSEELGFEMIDTYADMNLPNLRKPSLKGRNHRGLYHVHSSASINTTPEAVVEESNDTTMDTSNLARSMPDLHDNVDVISNDSNTIMSSPNSSLGDLVTSYPSDGSYASSIRNSSDYKDSLEIPNSAQSSVNAGSKLHQHVLLTENQKIKLLKSSYFKNETQFVIALQNISIRLSKVPREARLSTLKAELSILNKDLPCEIDIPMLLPKNKKGRLHKICRIAVNESAVLNSAERVPYLLLVEYLSDEVDFNPASSSNKKILRNIQDYDDISGSDKYKFDLSYKSPKRIISTASTADSTFKADGISDVEESDLADISVVKLTNKLNKSQKIELGSILLRDNASLPIPPLNALSDHEHNIATQLRIASIMLTQLDSPNSPLPQLQASQIKERIISSMQTMQARFAHNATEAAGERKLSNDMKVAGLSYLGEDWNTKKARIRSESRFGHLENWDLFSMIAKTGDDLAQEAFACQLIHLMANIWYQDKVKVWVKRMKIIVTSINTGLVETITNAVSVHSIKKALEKDMLEHNEFPISGVVTLKDHFKRLFGDENSIKYKIAQENFSLSLAAYSVICYILQIKDRHNGNIMLDSEGHIVHIDFGFLLSNSPGSVGFEGAPFKLTQEYIDLLGGLDSYYFKKFKLLTKEAFKSLRRNAKCIIDMVELMQTDSSLPCFKAGENTSVLLEQRFQLHLTDEEVDSFIDNFLINKSLNSAYTRLYDQFQLLTQGIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSF4</accession>
    <name>A0A1E3NSF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_26869</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019721.1">
        <property type="nucleotide sequence ID" value="XM_019162467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSF4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSF4"/>
    <dbReference type="GeneID" id="30179154"/>
    <dbReference type="OrthoDB" id="274944at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="3">
        <location>
            <begin position="19"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="104" mass="11582" checksum="CE52D11B1946AE87" modified="2017-01-18" version="1" fragment="single">NQNRQQKKNTYAPKRGPIIDLEPVLNKQVSVRISGGREIIGTLAGYDQLMNLVVENAVVKTPGHISYSEKDEEIKLDKVVVIGRLLLALEPMDGYEVITNPNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSG8</accession>
    <name>A0A1E3NSG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_90687</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020154.1">
        <property type="nucleotide sequence ID" value="XM_019165059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSG8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSG8"/>
    <dbReference type="GeneID" id="30181746"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="102"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="243"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="342"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="465"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="699"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="734"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="811" mass="91519" checksum="BDF4055A2D9B87A6" modified="2017-01-18" version="1">MSGDSLGTGIAEDPDSVPVSSENFELWIRMATDNKINTNNTWNFALIDYFHDFTVLREGDGVNFQKASTTLDGCMKIYSHRIDSAAKDTGTLLSSLNIKSKQNTISSRPSSSNNHAPPASGAGNLNKNNQAGSHNDDDGENQNEDDSDDELSDDAPDDGTIITGGNSKSKSKSKNKKKHTYLAPSFNTIKIKDQDRPVKPDPVFKNALANFDEGGAKSLLNNILRLSRESKVVFDIADDTELIIEDDSEEDEDGKESAQDEAHGGSDPTRGDCEDSIDINLLKRLVDFDIINHSLDVCPSLKELDEISNGKTDPANLLEYMDEVDVSRIENDRRLNIEGHINDTFDDNAQPDFMHDPDINMAESSDNNSNESDKMSNVSKRTQYSLYIDGVEDPDESGHNITFTNLFDENRQREQGKIREKDLIDATKPDKTDIDMIKIYDSIGTRPRLYWKIARLKRSTSNNRTFNEIDNDEEDTHNNRSSKYSQKSTSRAYNKKKDVVLIDFLSDKNDPNEEELFASTEYLSKIMLTEKERKSGRHLLDSDAPFTAKRLAFLSTKPNQLIKSVLMQKRKNTFEERELDNIPASEEFFAQAYRDENDNDILNDSIFSDPIDHPSSFNDNDDFDPNGIEGFEVPLPNPFEEQPRNTENMNAHPQTLYRTNAISYAKRSKKVDVKLLKQNIWNSIEEISIHKKKRFASHFEEEEGNGGGYSNKVQEEGTPLEVESSATRSEESTTRTSTMSPPAMDDFVDSGEDIDSMQFTNVVEKMSLKYNTKTKRDLSTSFCFICLLHLANEQGLTLETLSNNEDIIIHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSI4</accession>
    <name>A0A1E3NSI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDENN FLCN/SMCR8-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_48472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020145.1">
        <property type="nucleotide sequence ID" value="XM_019163136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSI4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSI4"/>
    <dbReference type="GeneID" id="30179823"/>
    <dbReference type="OrthoDB" id="5599713at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="1">
        <location>
            <begin position="39"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="228" mass="26161" checksum="B72B2280911BB54E" modified="2017-01-18" version="1">MTFIVSLAHFCEVHGPSMIMCTQAVEKPEELENFYSPQLSESQFCQSCMLKIPYAEEFAENLHFISTQFPTYTERYSSLRQTIVRVFTAETNADLSKPIMFGNSKHGYSIALSFSLLDKTARGSERKYSIIVTSNWEEDLINNYTFILTNLNQVVGNILRSARMVQKQLNLSADDLHNNGTYLRRSAGLPKAKSMVEILKDENFFLKIHMWAAFMLDTLIKQKEPNDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSL9</accession>
    <name>A0A1E3NSL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019686.1">
        <property type="nucleotide sequence ID" value="XM_019163588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSL9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSL9"/>
    <dbReference type="GeneID" id="30180275"/>
    <dbReference type="OrthoDB" id="5539371at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="10"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="351" mass="39364" checksum="B08377270DC65E36" modified="2017-01-18" version="1">MVSKLIFKGEKKKSRKNRVQKHEWQQTADSNKTSNSTFAINGKEVKIDDIDLAAITNGWTTAYLIDIQAANSSILDTQSGNLPIIITYADDSRNMCLMRGKLSSSEDASIKKKLIFSDDVELSTLENTIIYTPEGITVEADINRIEPTNVNEVFLLSDVSTLFKNTDKIIGNEHSNGVYNSRTPVYSLKTADGEYLTSDPSSNELKLSMTLTENGLFSLNFEMLGMTPYCRILVGKTNSNTMMITKSGDLKVIPDPEDVLGSLSRFIIRIRKQDAHRTKEILKAAKENSRSGDSDRDETAEKVKKTALDLSKLGFKINNKTLNEISKAYAEGWINEWVVDFKEKNIHDRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSM0</accession>
    <name>A0A1E3NSM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_26276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="5">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020214.1">
        <property type="nucleotide sequence ID" value="XM_019162439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSM0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSM0"/>
    <dbReference type="GeneID" id="30179126"/>
    <dbReference type="OrthoDB" id="10264154at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="16"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="4">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="121" mass="13230" checksum="2ACE278949299F10" modified="2017-01-18" version="1" fragment="single">KFTNEHEWISIHPDGTGFIGITNYAADALGDATFIELPAHQIGEKIENGETISSIESVKSASDIYSPISGEIVEVNEKLDQSPELINTDPMGEGWIVKVKVDEGSSLDELMDVDAYKTFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT55</accession>
    <name>A0A1E3NT55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_28892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019961.1">
        <property type="nucleotide sequence ID" value="XM_019162562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT55"/>
    <dbReference type="STRING" id="763406.A0A1E3NT55"/>
    <dbReference type="GeneID" id="30179249"/>
    <dbReference type="OrthoDB" id="423313at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF5">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="2">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="117" mass="13278" checksum="CD83377F28661364" modified="2017-01-18" version="1" fragment="single">FNPRQELEHILSTSPVVIFSKSYCPFSKKLKHLLKTEYHITPEPLIIELDDHENGKELQQHVGETTGRFTVPNFIVDGKSRGGADDILALHDNNELIDLFHQWSTGSAKIKKLGTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT74</accession>
    <name>A0A1E3NT74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020470.1">
        <property type="nucleotide sequence ID" value="XM_019160551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT74"/>
    <dbReference type="STRING" id="763406.A0A1E3NT74"/>
    <dbReference type="GeneID" id="30177238"/>
    <dbReference type="OrthoDB" id="3997979at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="556" mass="63386" checksum="13F660AF61F1046D" modified="2017-01-18" version="1">MYDLNACYHAGHIPVTAYGTNGTLQKDSSDNTFIGFDEHRSIIPGYIHKKFFTFKLPFSLLDTTCPDQLQEHMHLLPSFGFDEDSLGTLDENLEIDPILGYKRCEEVYGSPIKVNDMALKGQSISYFIRVQMIGRHDVAEKFIRKMPKSNNSPFVVLKNENYHFRVDASDYQATVNLSESVIGCNHLISRNIRTYEQLTHFENFVIKTLQELKTKKQLIEGGIVNRREQDEIVASLEVDESKKLTQFPSSTNFHEEIVRSDSDNYLHYDSMKLGKDLFGRSGGEVIIKASMSRDSSIHSVKSFALKEKHSHSNLSSKSGGGPELLPLRAIDNGRNHSVPIKSLSSDSSTSLDTIRSLHSLKQINQEHCFVDLDLTFIPDNRLAFKPELPQSLTVDSTLKAMNIYSTFPIPVSIDGEFLMDEQLLQYTLPNIRRQFDSYLIELKQLVGKVPVPRPFYNSVNSLSKLSAKEAYVPKFEFLSETINLQNKWKFDEKNNVYKACVKFPLAMGSKQISNSTICLVPTFESCLVNQFYMVHFHVSMKKSKKYCVFKFPLKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTH3</accession>
    <name>A0A1E3NTH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase Alr4036 family FMN-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020495.1">
        <property type="nucleotide sequence ID" value="XM_019163773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTH3"/>
    <dbReference type="GeneID" id="30180460"/>
    <dbReference type="OrthoDB" id="5394411at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024624">
        <property type="entry name" value="Pyridox_Oxase_Alr4036_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28243">
        <property type="entry name" value="AGL049CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28243:SF1">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE ALR4036 FAMILY FMN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12766">
        <property type="entry name" value="Pyridox_oxase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase Alr4036 family FMN-binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="299" mass="34273" checksum="5CE77C64DDC7C183" modified="2017-01-18" version="1">MLQSLALWNPSFTAAMQTELETGDSPFTIFNFSNVDSNNYPSTRSCVLRGFLFDQEKTNVLVFTTDKRSSKYKDLLLNPRFDACFYFHKLKKQFRFRGTVRMISDDLTPVIAYENPSAINEQSNDRADSDTSSVDDESQSLISQYLSLSNNEGSCQITEPLHYKLFSPSFIKSFEDSSVSLSDLGHRMTKTESVSKTPTAEEWENERLRVWNALSRNMRKSYKKPLPGSPLTEENRKLLDAINRGVDGNNSSTGYENFVVICMFVDLVDEVVLGGSGYDRRYLYRRTHNDTWKEHEVCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTT8</accession>
    <name>A0A1E3NTT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ASTRA-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020572.1">
        <property type="nucleotide sequence ID" value="XM_019163798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTT8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTT8"/>
    <dbReference type="GeneID" id="30180485"/>
    <dbReference type="OrthoDB" id="7668193at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="53848" checksum="BEC920EF8C1F211E" modified="2017-01-18" version="1">MVDKVLRATLRAHSSPITCIVPFYIPSTLTYTFSEDETRDYPLLNPSLMTADESGLVIWWNILTRRPLGLWKAHSGSILTIEQLGIDWESNGSHDFSIPKITESYGQLLTHSKDGSIKIWNMINLVEGSTAKSGFTYSCLLKKKISLADSEIPVPTPPLLYEIPVNAMNFTNVDMNLNGYLLTPATTDSEGWDLYKINLKEIEEYKKLKRLIQNHQPNLHSTEKMVEIGEDDQEAMGFTKRGRLGVIMKTAWIGLNRFLVGYESGVVVGYEVYQEVNENDGNLSTQTKNFLYNNDLEGNPITAMCIDKANRKILCSSAGSKVSIMDLKSKDYCSKIFETKHKGISSIDCDSFSNAVGLITWDGYTRFYNYDEKLVLKFSSKMRRQLPAISNSKEVIDDQNSEVTLVNSLQTQRASVIKFTRKQLDPREAKLAGMNMLYNNGRSKNIVKRNKEEVFGERWLFVGYQDGKVAMYSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTU2</accession>
    <name>A0A1E3NTU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_30609</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020086.1">
        <property type="nucleotide sequence ID" value="XM_019162644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTU2"/>
    <dbReference type="GeneID" id="30179331"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF20">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="7">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="183" mass="20890" checksum="65EC3A2FDF578101" modified="2017-01-18" version="1">MAFQYVLTFSLLVAEMVMFSLISFPLPSKIRRPLLKALSIPFHSQQFQVVTKCVFVFIGIMFADSVNRTMKVTNELYNGTLNSNDMLNGGVSRAEIQSRRFYSQRNMYLCGFTLFLSLILNRTYTMVFDLLEVKEKVKKLESESGSRPAASDANNEKLRAKITELEAEKKALLKKSKALSDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTV7</accession>
    <name>A0A1E3NTV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14924</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020592.1">
        <property type="nucleotide sequence ID" value="XM_019160599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTV7"/>
    <dbReference type="GeneID" id="30177286"/>
    <dbReference type="OrthoDB" id="3053196at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF11">
        <property type="entry name" value="CERAMIDE SYNTHASE LAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="11">
        <location>
            <begin position="169"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="409" mass="48605" checksum="D143488BC8CB0F97" modified="2017-01-18" version="1">MTPPAAAKKNAVVRRRHSSVGKIDVGDNSVHGIHSGRTNKDITAAEAEQKRHLNEVTSDINDTVVFQKLYYSVKILCYRYIWLVPLAIMFSMNLVYFLSNNYTPSNPLHRFLCLSYQIPGTHDPVKYDKGWMDFCFVGYMMIFFTFLREFMMQVVLRPLAQYFGLQSKSKISRFTEQTYAVFYYGITSPLGLYIMKNSPMWFFKTSEFYRLYPHRQHEWLFKFYYLFQAGFWSQQAVVLLLQLEKPRKDFKELVYHHIVTVLLISLSYMFNYTWMGLAIYITMDVSDFFLGLSKTLNYINSPVEMPFLALFVVVWVYTRHWLNLKILWSVIFEYLQIGPSVLDFKTQQYKCIISQPMVFTLIMALQLVNMYWLFLICRIMVRVLFYNIKKDERSDSESDQDQEQPQPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND91</accession>
    <name>A0A1E3ND91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED11</name>
        <name evidence="5" type="ORF">PICMEDRAFT_74925</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015200.1">
        <property type="nucleotide sequence ID" value="XM_019164916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND91"/>
    <dbReference type="GeneID" id="30181603"/>
    <dbReference type="OrthoDB" id="5418434at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019404">
        <property type="entry name" value="Mediator_Med11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10280">
        <property type="entry name" value="Med11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="116" mass="13025" checksum="44D0E1226D32DCA2" modified="2017-01-18" version="1">MASTGEQTDTEYVRERLDAIQETEQRLVSFLGTFAEVMEKVEETHVELLDKEAGAGDGDGDGETVQQLMSRCYEDLSFASVHLRRELKLLEVKLPLPAGLSKKASDVNNEKLRRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDW5</accession>
    <name>A0A1E3NDW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine metabolism regulation protein II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_46504</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015434.1">
        <property type="nucleotide sequence ID" value="XM_019163124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDW5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDW5"/>
    <dbReference type="GeneID" id="30179811"/>
    <dbReference type="OrthoDB" id="3477330at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="97"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="837" mass="95965" checksum="EC22BE162879E170" modified="2017-01-18" version="1">MKCKKSGFVCEGYDVKLRWSQPIEFDKYGYQLPSKPNQETEYCQRRSIEYVQYSDRAAYKYYDEMDTDLASLHNRHLESKGLATVIKGHFGVFRGTKLTPKKKRRRVNERAGEKQATKGTSLQSAKRKKLSDLTPIPGTQFKPAKKNQNFLYPQNVSTSTMPNSGQFDGTKQGNSMGNEWLSTELLDAALLTASALNGDTHFLDMFDLDNSNALHNNNVINPLGSSGDSGSHHGSHHNPSHSSTTDLFSDSVPDKMLHLLFQGRNVMDVPKDPQHIVPTTNLNNKFKGINQPVIGNEIKIHASTGIKMPKNIMEILETTPLPKSLAIDKFGIPTTSLNVHPMARYLLNYYIEDVADMMTVIPLAKNPWKFIYFPRALMAVGELASLGRTSNARNGLLNALLAVSAFNLQSKFVRGSDEMKYYVDLGIQLRRQANEFVDRCMEEDILSQKYKDVLVAVLSMVTIDVVWGTMSSCKIHLDHCEKIIEKKMLHKKKLSSKAIILHRIFSSLKLIQDSTSLENIQKSEIFLNEKNYKNFLTGSKPTKEQLDEINKDNFRGIFRGKYNEKITDNGKVRIEFIVNNEESNLNNEQFDANSDIPAFVDITRTSFKPSKNKLEDKVVSSDAIYGLPNSLILMFGEIVPLTRFKKYHEDHNIMLPSFFDELATQLEFKLLTWKLEWTLIVEDDNGSSIGDSVDEDKRQFISPRHEGIYHHVMSFYHALIIYFYRFIEDVNPMYLLERIEKVLHHLNKIQDILENNKETSYIIPLFWQGFIAGSEAMTLYLQNGFNKWGQDIAQTGIGTYWNARQIMLEIWRRKNHNVKNNSWVDVLRDWKTNVMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDX2</accession>
    <name>A0A1E3NDX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_65417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015427.1">
        <property type="nucleotide sequence ID" value="XM_019163486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDX2"/>
    <dbReference type="GeneID" id="30180173"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="926" mass="108026" checksum="8C9317322B90433F" modified="2017-01-18" version="1">MQYLKRQGLVSVAFGQHSRGVRTRTPIKIDLTKGKNKKKGAIDIQDKDPGVPVSDGKVERSRFSRFSGTSPSHSFTSTPSSSSRNNEYLNEVMQEMKFKKPQDIVNELTARFKQDGVEVDTDFINRMVQESAKEQELVSEYIKDPSMTLSKVSEEEINRYIDWLIEDGKSRISDQHRDTYREKVEIRKKLDEFMTSDENETGSDPEAMTKAFAMAQELSVMDSKSSLNKLPLFLKHLTKMSNVDLQSSVPLEKYAALYELSTQIVDSDRRERCIYLCGKLLYQALRRDLGPRARPDPVNEKFFIESCIRYNDLDRAFFLYDTRKEKDVKNERFWFELGVCIHLSRYFNESTSLPDNAGENNKNENQMENEDLSKAIDLVHDIRDRWGYVNNLTLIDALKKCCMKENFDDAFWFWEDIEINIQNLGIVKEIVVPETRLYDDKDREQVYNYYNRTEPISYDGILECIFSFIGGLQFERGVQMLKNVVEIDEEFIYEFVRRFGEQFKYSGRELFLITLEENSRVNYPSSHKLSTEIRDYLIEEISTLQKTRCVSYNEAKIVEDINIYLERLAKLKSKHLSKINDLQEIIQSGEKLTSFDVRSLLEILLQHKSAASFQLACRLIGQMNEHKSNGVVDSILPVANSYAYTEFCKLFLIQSNPRVKEINDLLQMMVDYDIKMEQELANKIILSFISKKLYSEAIKFIETYVFSDNPIAVNHIRLGKPGTKNLWTTLFCAYYKSVVSGTLTGDLFSLRLNSLRSLMKKMMEQEVDDNFTIQEAVGTLLAYGDFPGSICLIQWYGKLVGEKNIKFDFVLAIKTKFELSISKAERYLKHTGKAAHQYEQKIKQYRHHFGVQSLHDDFKTRRDFSWQEVAMVLYKYAELFCYKSTYTKEDPFSLLLPDSERYHNKAVFETNLSGLQRFYNLPEWKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDY0</accession>
    <name>A0A1E3NDY0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Delta 8-(E)-sphingolipid desaturase</fullName>
            <ecNumber evidence="6">1.14.19.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_18244</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Delta(8)-fatty-acid desaturase which introduces a double bond at the 8-position in the long-chain base (LCB) of ceramides. Required for the formation of the di-unsaturated sphingoid base (E,E)-sphinga-4,8-dienine during glucosylceramide (GluCer) biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an N-acylsphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = a (4E,8E)-4-sphinga-4,8-dienine ceramide + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:46280"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:85953"/>
            <dbReference type="EC" id="1.14.19.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.18" evidence="6"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015437.1">
        <property type="nucleotide sequence ID" value="XM_019161921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDY0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDY0"/>
    <dbReference type="GeneID" id="30178608"/>
    <dbReference type="OrthoDB" id="260091at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF30">
        <property type="entry name" value="DELTA 8-(E)-SPHINGOLIPID DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="19">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ44324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="543" mass="63696" checksum="15CE5799E92E071B" modified="2017-01-18" version="1">MAVKQNEKVFTPRDVENLIAKGHAIVIYDHYVLKLDHWIEYHPGGNKAIYHFVGRDATDEMNAYHCDETCKLFTRYKIGVIEEPWENMIPPIQGGHFRKLDHNDEDKSLGKGDKTSKGKKLCGEVDAKIEPKIPQGVILLEGMEEYYPTKRDGPIRDPRTIIDNYDNDLVKRDLNDIPPLDYKTQRGLSRKYNELHQQIINANLYECPYYEYFLQFCINLSLGLYTMIFFKTKHYFMSAVFLGFFWQQLVFIAHDSAHISITHNYQIDNIIGIIIADWIGGLSIGWWKRNHNVHHLITNDPVHDPDIQHLPFFCVSSRLFGNVFSTYYEKYLWFDVFARKLIPIQHLMYYPILCFGRFNLYRLSWEYLLKGQGPRHGKAAWFRYFELVGLSFFTYWFFYLVVGSLPTKWDKFQYIMVSHITTMIVHVQITLSHFAMSTSDLGASESFVSRQLRTTMDVSCPDWFDFFHGGLQYQAIHHLFPRLPRHNFRKAQAYVIDFCRETGLKYSIYGFVDGNGVVLNKLQDIAQQVVIMKECIVESAKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDZ1</accession>
    <name>A0A1E3NDZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015455.1">
        <property type="nucleotide sequence ID" value="XM_019160076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDZ1"/>
    <dbReference type="GeneID" id="30176763"/>
    <dbReference type="OrthoDB" id="5204833at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="495" mass="55185" checksum="C22F4709C51756CC" modified="2017-01-18" version="1">MLQPNSSPWKRISSTLRSSDYYSPTKKTPEKCQQKAELSANGSPLPSTFRFTPTKGKKLYPHLNPPDRRLLPNSSPSYSDNNDHDESYMVKDNTSFLSAMDTLGKQLFSSNISNKVVNEFSSRYEKVREESISTVQELNNRTTNEQLNRRASGRFSSTHRNRFNRMESIAFHYAAKRSQKLENEKNENEITNSGAKKENHNVQRKFVPATETIEITQYSTLTPGDKRFNSKPLESASKRLRLEGNKFKEIDLDSSPTKSLYEKEQLKNIYSTHSSATRDSSSSHSGVSNIPSYLQPTKASLRKSNSSSQISPSKSSKSLFNALGDQLKTPVLRPQDASLKKGLSKSPSFSQPNPLPGATSQNSSPMQKISPSRACSNLNKLLNTPPPLEAQENRPLEVPQLKSTTNSHANVNSLHTKIPRSRTLGPIYNDSTFIPSSKTSKISTSKSHVSSVSVNNLRSLSGSSSIPRLARLANAESRVGLASKHEREKPLKPWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE50</accession>
    <name>A0A1E3NE50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_18319</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015523.1">
        <property type="nucleotide sequence ID" value="XM_019161949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE50"/>
    <dbReference type="GeneID" id="30178636"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF45">
        <property type="entry name" value="NOCTURNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="4">
        <location>
            <begin position="136"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="520" mass="60420" checksum="7E63163E0868D08C" modified="2017-01-18" version="1">MDFEGETDGQNQENQVQIDKDALISGTESLSLTNSTEKPISDVIQNQNGDQKKKKKKKSGKVPRNMITPEYIEEMRKQREEKKREKREALIKQGLDPDAQINEANYLKRELLSIPRRGGEEDGEENRDSIITLKIMTYNLLAQALIRRKLFPDNGDILKWHKRSKVLFQELRDYDCDILCLQEVDFVQYKSFWRPELEKLGYQTKFQRGADKNHGVSVFFKHSLFNLVDTCLIDFDNEKSGDVKPRTITKNVGLILGLAIRNDPSKLITIGTAHLFWHPFGTYERTRQTYVVLSKSKEFERRIQILHPEITKIWKFFAGDFNSQPYDSPYLSITSKPICYNDRCMRVIACSTSFQFSSLRNGGSGEEEEGGNVEKFGENQPKDPVPESYVATEEQQLMVKQIEQLHNDLPLRAISLYSMAYREIDPENSGLDNDRNEPFFSNWAHTWRGLLDYIFFVKEWDVKSDHRNIDSIPAFERENKVRLDKLLKLPHPKEMGPGQPRENEYPSDHLCLIAQISLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE87</accession>
    <name>A0A1E3NE87_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015547.1">
        <property type="nucleotide sequence ID" value="XM_019164840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE87"/>
    <dbReference type="STRING" id="763406.A0A1E3NE87"/>
    <dbReference type="GeneID" id="30181527"/>
    <dbReference type="OrthoDB" id="3998205at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="186"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="866"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="666"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1054" mass="114891" checksum="6AFD7DE667ED9C6E" modified="2017-01-18" version="1">MSNTPVTNPVNWLDSLLGNSSPAVQSPTQSGFEAAAPEGYTTQQVLDVYNRMTQTGRLQIDPNAAAANSARASSLHTPLSVELLNGSSAKAAAAPGPAAPSAAASAAALHLHHHQPHQLDSPQSTSATLGNPSSALNSSQATPKQSFVSTNPLLSNMAAGINRLGSSTPEISHIDHDMAKFVQPAAVQWFYQDLAGTQQGPFDSLTMQQWYSTGLLVSTLPVRRDGEANWLTISDLWKKCKSLPNFDPDLAPFNQPLPVSLIHQPSLLTPSGLHNNSMLHPNPLTSFNSASSIFGSANNSQFFNPLSSLSNNNWPQSPLNETSPKPIDPTVNTNEGSKEPAKDNDHPATLDSRSNSNFNSFGVPNMSPMNMNPMNMNQLGMNLNSLNLNMGGMSGMGMGLSPMDINSMNPMGLNPMDMNAMNNMYINPMNPLSQMNMNHMNSLNPLNQMNTMMNNGLGSRSNSGMNNISIPGLNANSLNSVNMDGSMGLNDLSMTGINNIDVANMDPNSFFKIGADPHVSKLVPSTTTTEHGETSVQREVDEPSKAAPALEAKEQATEISKSHYQTKNTSNFEDQVKIAKQQHYNVAREKEINDMKAREEEKKKEADEMRRKLIEENGKEAQRQIELKRRETEETQKRAVLEAMKLEEHMKSLELENKKKLKQQQKLKNQEQQQKLKIADESASTPERPAAMKKTKSDIAPWATVSQSIKPSKTLEEIQQEEKSKREIELKERRRLEENDRLLASRLALQETLPTVNVNGKTLVSLSALSSNASAKPKLPSNSTWAINAVSPQLPTKSLDEIQEEELEAARKQAEAAAVAKVQKSIAEAISNQQKTFANSIANENSSTDNAWTIVSKKLRPTIATVSSPSKENVTKGSTLNPSTLRSMSAPMHSTTAAKPIAASATSSYIAPTVPSVSISSFPPLVMDFLGWSRTQLTGLYSSVNKEDVLQIMMQLPVGTETQEIIADTIYSNSGTMDGRRFASEFMKKRGKIEDTIKKRGWAFDWFEALEGTKGMKVSKVTSIGNSGFESSADNDDWDGAFTVVSRKKGRKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEC3</accession>
    <name>A0A1E3NEC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein 14 C-terminal Fig4-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74713</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAC14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015593.1">
        <property type="nucleotide sequence ID" value="XM_019164847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEC3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEC3"/>
    <dbReference type="GeneID" id="30181534"/>
    <dbReference type="OrthoDB" id="3996793at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="6">
        <location>
            <begin position="512"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="746"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="777"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="821"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="831" mass="95133" checksum="25C31B88E0FE47A3" modified="2017-01-18" version="1">MDKALKRGLTDRIYEKRKATALELERIVSEALSSNEQEKIQLIIKQLRNEFAYDIHEPQARYGGLIGLAAISIALGPNELPQYLNSIIHPVIACFGDNDPNVRYYACEALYNIAKVAKGEILIYFNEIFDILCKLVADVENSVKNGADILDRLIKDIVCDKSTNYISVLNLGKLKAKDPTVQDKSGNNLQYYDVQSTKAFSLSKFIPLLKERIYATNTYTRLFIVSWLILLDSIPDLELISYLPSFLDPLISYLKSPLKDVRIITENFLKLLLQEIKKIHEIKTLAKEKRLENEPDSSNSDEGIYLPGQNTIIDYPKIIDILVNNLESNEDLIKLISLDWLINLLTISPESFILLIPKLLVILLNIISDNNPHLQEMSLQLNSSLINLVSTNQYETNYTLLINNLSMELLTNQNQNKGTQKNNKKTSRNSTNILTRLNSLDWLIMLQQKDPVRFMEFTDNIFITLLKTLNCSNEKIINKVLDLLSRISNQSDDKYFNNFMIDLLDLFKKDSKLLDSKSDFIIKKICKSLDSERVYKSLSQVLLDKFSNNEEDLKFISIMIQILNNNLIIAPELNGLRKKLINNLNTKEPIDLFESLFKCWSLNSPSLLCLTLLTSNYELSYRIINQMVKYEISINILVQLDLLIQLLESPIFAKLRLDLLDPITNINLFKCLYGLLMLLPQSNSFKILQNRLHAISSITNLPLNKDTDTSNIKTKPETIINGIDENYNIKLLDYFNECQERLENLKLSDSKDGANDDDDEENDNGKDRTYDSVIEMKNPKTDTDHTNEAGLEGTVTGKDVNKGKYPSENYLDMFQGDHPLETSSIFNQPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEG9</accession>
    <name>A0A1E3NEG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015648.1">
        <property type="nucleotide sequence ID" value="XM_019164724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEG9"/>
    <dbReference type="STRING" id="763406.A0A1E3NEG9"/>
    <dbReference type="GeneID" id="30181411"/>
    <dbReference type="OrthoDB" id="10050400at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031942">
        <property type="term" value="C:i-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040201">
        <property type="entry name" value="Mrg3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="430" mass="50183" checksum="45A4A2F2F810219E" modified="2017-01-18" version="1">MLKRTNKLYQLAKSVSRYVPPSQRAIIQHKEYAKSKRQSDVLGKIVRYGAFTLVSGSILMYLWQPWNPYSKEVSKELRKGLWEERDGNEDYLNALKYYLKAMKVAKENEHMDQLSMQYTGIVLKVAEMYQNLKMIENLTATYYNLSTFIFENLIHGTIKDDNPEKELLIDRDLIVITRWAMLMQNAKSKNWVSDVSNELRDRMAFIENNEMLNDLPWIATDPKSKKINTNDLIDIWAETKVDRFGINHTKKTEWIEENINSEEGKQYLECWDIMRSFKDRNWPVWIESYLKLRDFYAMLQMNRGNTTSCIQILQSNLLWSTVAGFDNSVNSTTQIHNLASAWFQLGQQTNDKTAYANSRAIYEKLISKVTTKDPILPISYYSLGVLALQLEDKKSATQNFSKARALAVDLDQLQIVDKIDDELLKNLKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEN5</accession>
    <name>A0A1E3NEN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_18000</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015695.1">
        <property type="nucleotide sequence ID" value="XM_019161842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEN5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEN5"/>
    <dbReference type="GeneID" id="30178529"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="637"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="140"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="773"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1160"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="795"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="809"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1163"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1197"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1242" mass="140321" checksum="E7846860289D131D" modified="2017-01-18" version="1">MSNTVTVVNNGEGGRSGGFESSIQTPATESGLLNRASDRDAVASSQTSATSATTLLSSDADDGKKQSSSSKVKANSKSSSSNNEISVGEMNYPNSGGSAADEPHEHGEGSGDASGVSVGIKQEDVPDNDKIKDKKGRSTSCFLCQKRKQKCDQRFPSCTNCLKSKFQCIQPPRYGENNNTSGKSNDYTKFLEKKIKQLEKLLDLKAKNKLNDMKNNRTKRITLNDEKILKYKKISPYIDGEEGGSCDAGAGGRGRSNSSSVSSTSDTDDYNSDVSLINQPYATFMSINASAFKNSVLKNYNLNDFIKFDPILNIDQKFSKNLIGLYFRMLQYKFPLLNEREIFEFSESYYENKKQQQPLSDEEFNVYHFKCSRMFFIYAISALLYKSTGKYKGPNPFNFFSTALRHLILLSNNVEAFNKIELLILISFFLIRADKNSNNLYLIISDAMQCCMHLSLHKSKSYENIDSNLKDLKMRKFWCCYILERSILISISKPFILHERRVDSKIPLFNYEPSQAFNPNNGKIFFINQSIKIKRLESRFIEELSIVTASSKSINKNQLVKVERFFQELQNWRNECQGFNNNGIENQTLLFYYYKSIRHLIQPYLELLDPNDKLFKECQAAAGQICQSIKNYHAKTFYGFSILNIHLVFIAGITLIYCLWLQRNRDDMRRKLLGDDKKHTRPTVSEDLFRGLDDLRACSISLYVMSERTKFALSFRDTFEEIMNATIGNLILRCGPDSSEILYRGIGLPPAIFRKPLKHFQIESNYIEKSDADKLEDEERQKRKGHLTRSAIPKGLSHLLLHPPGLEDYDQKDNEKDRERENQMRKVQQDLEKERKRESESKEREQERLRQGETQREQEKEKENEKEQEREFFRAEQDRSDLNNRILPLPIKLPQLQPQTRLTSSLNSKSFDNEIRLASGNPYAMNNLSLKRKYSKCDSETDSVVSSLPSNVLSPLVGSTFKSSLNTPALMSSSLNTAVSNSATAPNNELSSTSTMSDKFRYLRSPLNPNSPNIVNNTAAATITSSSSPDTSSPMSTNVMSDNLVMQNTIPDLIPFVGGTTNMISNISTWTEQSGQQVPQTGLTMLQYDSHPNPNEMSFSHNMSPANLGQNHNNHSHNLAHPHHLNNNNNYPQSHLPNSTNSMSGYQSMGVHLDGSHNGNGYGVYNNTNNNNNSNGDTYNQNTANGSAANRNNGNGMDSNKSYPPQYPSQPNNGMTETFNSLFDVVGNDDFWGLNNDLGFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NET5</accession>
    <name>A0A1E3NET5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_174556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015753.1">
        <property type="nucleotide sequence ID" value="XM_019161610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NET5"/>
    <dbReference type="STRING" id="763406.A0A1E3NET5"/>
    <dbReference type="GeneID" id="30178297"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="728"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="869"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="946"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1090"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="520"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="661"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="915"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="946"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1122" mass="122778" checksum="663E17CBA3789C58" modified="2017-01-18" version="1">MSKEVFNEGPTTSQHVESVDDNHFQNTTFKLKRTRSMGLLDNFIVDPAAAGSANSSGSSSSSSSGASSGSLPSSANTNRSTLGRIQPSKLTAGTYIPEETLTRLNLKSPASSTSCSSHAAGSGSNAGNLFRSSIINKKLENSPQSFQSDDFSDDLYTSEDSLSSPGSSVHNLNSTSNICLIPKHQQKRYNQLHSHDSEENGYYSSVTDSHNSNSQPISSNPTSSSQDFTDVSNLSNMLHDDIDVKDAPNEHVDYLTHKWAEDEISKSWKYVILRRKNVADSARLENASWRTWAQTRLGLKTISPEDLNWSKDSDVTWLYGPVIKSSNSNSNSNIEGNENVKSGSNSTTPLSPPSSSNFASSSSNENPSMSSVTLLRNHSMDNESPLIINNNTNKSYDNTSNDNTSQNNNNNNKKNANNNNGDNESTLLSIDGNDAHHKSGNQALFKTGNNSCEHLKSILKKKTKVEKMISDASYCRLQHLLENREQKLKSQHESSVSPVLEPSTSNSLNSIPSTSTITMSNMNLLNNSSNLNSRSNSTLASSKTLHSKKKSSLKNSQLHIQQNSSEVRSDSNTKKEKHIHFNMRVDQCIALAKPNKGGNNKKDDKERADNVQDDTMGYANSSDESDDYPVSKMVSSSYKEDHDHYADDESDHQFNSNAAMDDSDSDSDSDEGGFVLRPSVNKPNISHNPHENNYERNGKLSGSRENSQNSIIVVSTIAPLPATTLKFGSDDEYDNDDYGNDGTASDGSKYPHNKMYTVSHNTKTNRGYDYYYDYNTVYSNTSNPMFYSANNNANADVEMYDVPESCQIEDTMDLDDNQSHTSPNIADSPLISHMGYSLPNVHSVTASPLMLPSSVSDNTMVDVPASFMNDIPQFEDDNNKHDSDIDNTDTTGQSMHIERSDMDGPTNDGLKRTRSIGSSSSRNASSQSLSSIKMGLSGLDLNTSGLRRSSATDSKPQLFQLSNDESEKSNLSTAGKSGKSFISNDSSKNNSARSDGGSGRNKFMFAESESETEAGTDDQMDAEDNYDNGNDYDQDYDEDYNGYSDHDDDDHEMIDDRSIENVKRSQTNLINAVKSASSFGSLAEINRKFGGIGATSNPATTNSKGKPRTAFSFQNDSESESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEZ3</accession>
    <name>A0A1E3NEZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase Rpb6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_22696</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015807.1">
        <property type="nucleotide sequence ID" value="XM_019162273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEZ3"/>
    <dbReference type="GeneID" id="30178960"/>
    <dbReference type="OrthoDB" id="259769at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00192">
        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028363">
        <property type="entry name" value="RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002207">
        <property type="entry name" value="PRK01099.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500154">
        <property type="entry name" value="RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8720" checksum="241D24FCEA4B0FE4" modified="2017-01-18" version="1" fragment="single">ERITTPFMTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMKELSAKKIPLVVRRYLPDGSYEDWSVEELII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF38</accession>
    <name>A0A1E3NF38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_13420</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015870.1">
        <property type="nucleotide sequence ID" value="XM_019160056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF38"/>
    <dbReference type="STRING" id="763406.A0A1E3NF38"/>
    <dbReference type="GeneID" id="30176743"/>
    <dbReference type="OrthoDB" id="5418899at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF12">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="369"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="258"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="442" mass="48193" checksum="DFBECE2C75291DB8" modified="2017-01-18" version="1">MASLYRHTSYGSQENTANNSQNNNNNNNNNNSNSNSNSNSTAFSTGNSMADMNQNYHNYPNMNMPPAPLPNMNMNMSMSKVPSMGGMNSMNSMNSMNNRSMGVMGVPMGYSNQGYPVSGGMNLGTGVGVGVGIPVSAANYTRQHFPPSNNVGLASQLSYGEKDGFDSRIPSGGITSNTQTKSPLSPQLYQQNNTNRSSGYNSNGITIGVSKGSVAYPPTPQTLHSQQGQYPLTPPAYDNQIGGAGVNYRETSRLRGLADEDSEGYGDHVSGSSNTGDESRELYYEMSSKTYVPKTREEGREGLRTGALVKVDAEHENGDSSGSRKKKLTGSGNESDDDDDSDGRDDESRNPEKREASGNRSRNKRSRGGCLTCRQRKKRCCESKPVCSECRRLNIKCRWPVPGSERKNKSKNQPHMSHDEVYHEVYGVIKVLRGVVDYKIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF41</accession>
    <name>A0A1E3NF41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Uracil permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_36702</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015852.1">
        <property type="nucleotide sequence ID" value="XM_019163027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF41"/>
    <dbReference type="GeneID" id="30179714"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000001">
        <property type="entry name" value="Uracil permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF2">
        <property type="entry name" value="ALLANTOIN PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="553"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="71090" checksum="DCDCA7D2F3E75B55" modified="2017-01-18" version="1">MQDVKEDFTIHESSGNLHERGGHGHAHQGSSVDKSPAEVSQISSGSRDSPFVGDGGTADSASGSSSGADSNNGKKREELFVWRWLRPLEIKGTQGLSLSQLFLLNYDLRPVEEARRKWHWYHFVFFWIADSFNINTWQIASTGVQAGLSWWVVWITVWLGYFFCGCFVTASARVGNAYHISFPVSCRSSFGIWGSLWPVINRVVMACVWFGVQSSIGGNCVELMLRSIFGNDLDTRIHNTLGGNITSFKMLSFFLYWFFQLPFIYLPPHIVRHLFTVKAVICPIAGIAFLVWTLVKADGGGPVIHQKSTISGSEFGWAFVNSTMNSLANFATLIVNAPDFSRFANKPGAAIWSQFIALPTTFSITCLIGILVSSASTVMYNETYWNPLDVLSRFLDGYSRGDRGGVFLISLGFAIAQLGTNISANSLSAGTDMTALLPKYMNIRRGGFICAAIAYAICPWNLMSSSSMFTTYLSAYSVFLSSIAGVVFCDYYILKKGLLYLPELYIANNSSPYFYSKGVNWRAYVAYICGILPNIVGFVGATKTHEVPMGATYVYYFSFFTGYAASSSVLLILTLLFPIKGMPEDSKVFEFKFYEEWQEVEDFDLYLRNKDLDLGIIDADDNDTSSSIEDAFASPSGEKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF94</accession>
    <name>A0A1E3NF94_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_36991</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="3"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015938.1">
        <property type="nucleotide sequence ID" value="XM_019163046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF94"/>
    <dbReference type="STRING" id="763406.A0A1E3NF94"/>
    <dbReference type="GeneID" id="30179733"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF269">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 5-PHOSPHATASE INP51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="6">
        <location>
            <begin position="158"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="943"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1015" mass="115618" checksum="7DA55BF1537DF4E7" modified="2017-01-18" version="1">MELYINDSTHTLAICSNNYALILVDSLQNRGVYAGSSNYLYNHSHSNHAVSPDGTTPKCLIEFVPRNTVPLHTFRRSPIELYGLLGLICLKGTMYVGFISGRANVGPLIPQVNVHQLVSTIFVSFSGEVYSLYNQYSHGSHSQQPLQQLQQLEEITSIMKLLETGTFYYSYDTDLAVKSQDWDIANKSDYTYTTVQSLQDDYAGRFVWNSNLISEVSIFRGRLADEERKIFDQGKFYITLIRGYVHREPFDVRDDSWITIISRQDCRKVGPLFGPACVDDEGNVANFVETELVVYTGDNLVSFTIVKGNVPLFWKLDTHLLSTKIEFPRSEDASKHAFNRFFETLCTEYNMVYVLDALSSKGSQPELSDRYANAIQELQTDYKELSVGYKKLTYGVSLGNRLKGKNDYMSLLLQDEQIHAALQNYSANQFNFKEWKQSSRQLGVFLVNTLDSNDRANHVQCKISEMILEHLFGTNFTPEVWSKHNLLWVMNGTALGKLSDTYNASIKTKNKTGGLIGKFAEQGKKYAEQSKKYVSGGPNNINGSSSGRQTQFDRLLGRKNKEFQVELIDPIHDYVLEGLNKRRNEFTTIKDLLIYTVTFNVNAVLYDGDLNDLLFPEEESYSKYDIITIALEEVIELTPSKVMSIDLSTRNFWERRFKEVLNKKHAKEYTLLRGEQLGGILLLVYASIEIIDNIKKVESSVKKTGFKGISANKGGVGITFTFSTHSRLCFVASHLAAGQSNSEERHQNYNTIATGLTFKRCKNIKDSDILIWMGDLNYRISLPNETVRKILQFRTPAFALKQLFEYDQLNAQMANGQTFPFFDEMEISFKPTYKFDKGTDYYDTSEKQRVPSWTDRILTHTKDKNVTSLEQVRYNSIPKYKFSDHKPVYGIFVAKLEIVNDSSKSVIEKQLYDITKQKLYASETGGVLLPGILSSLMSESRSKNLVKRGLPPPSSKDSRWWISKNKDGNMSDEKIGKVKVRFPELDSGEKMINPNLPKNPFTKTTEPLFVVREGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFI9</accession>
    <name>A0A1E3NFI9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit WBP1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_19879</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015216.1">
        <property type="nucleotide sequence ID" value="XM_019162144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFI9"/>
    <dbReference type="STRING" id="763406.A0A1E3NFI9"/>
    <dbReference type="GeneID" id="30178831"/>
    <dbReference type="OrthoDB" id="29105at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="10">
        <location>
            <begin position="220"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="373" mass="41542" checksum="38A054EDC66A5D1E" modified="2017-01-18" version="1" fragment="single">IYDLNENIVVSNVVILPNKAKKLASNIDHASLLTYANNGGNVVVVSEDSGTQLDVSIFLNQLGIYPSPKGYKYTDFHSGSAAPLAKIEAPRFLNKHVVSEDVSGLSLEDASVALVSNSEYLVPLLKASRTSFTTNGDAGKDSVWHTGNEGYLAVAFQGLNNGRVLWLGSPAPLKNTSFNKGLVDSVVQWTFQLKSVIRATFAHHKRVDASGESDLPFVDEDDYYKIKDTAYFEIGLSRYEADRKTWVPYTVDEEDVANKVQLEFIMLDPYYRLNLNHTHNTDTEAVYSALFRLPDQHGMFTFSVDYKRPGLSYVKVEDVVPVRHLANDEYARSWEIPNSSVYMAGYGVVVVGWLAFVVLFIFSGKRNLDTAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG84</accession>
    <name>A0A1E3NG84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_59915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016209.1">
        <property type="nucleotide sequence ID" value="XM_019163400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG84"/>
    <dbReference type="GeneID" id="30180087"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="106"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="34"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="513" mass="56243" checksum="107824B628811D91" modified="2017-01-18" version="1">MTGNTKDDYELSEDVVSFRPHSMVSNVIGRASSSIRRESGVYYSEDDEDDEDDEAADPEDVFESIFDGDVSGINNDTEKRGNDDDDDENHGSFEEHVVEYPEGGKLAYITLLGCFMGLVGEFGLWNSTGAIESYIAKHILVDNSDIAISMIFALFSFFVMFGTMISGVVFDKYGGKMLCYTGCIMTTGGLMATANCTKLYQFILAFGVTCGIGCSLLTAPFVTSIGHFFSKKRGMALSIAMPGASLGGVIWPLVCRSLYGRVGFEWTMRTLGFCIGSLLFFAATCVHDRHVEIIRIKKLNEGERNMSLREKLNDLIDFSSFKELTFMVLTGALFLNEFALLMVSTYIPSYAMSKGFSESFSLIALTVFNAAGIVGRIVPTFLSDRYGNFNFIILMSTVMTITVWVIWLPFGHNLGAFVAFTIIFGFAVAGTLALTPLCTSAISKPKDFGKRYGTAYFFVAFCNLISLPVGMALTETNAGYDAMVAFAGATCTLATLCFIYTRYRVGGLNKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGB2</accession>
    <name>A0A1E3NGB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA ligase</fullName>
            <ecNumber evidence="12">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_36304</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="12"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016239.1">
        <property type="nucleotide sequence ID" value="XM_019163000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGB2"/>
    <dbReference type="GeneID" id="30179687"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000016">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="14">
        <location>
            <begin position="360"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="621" mass="70548" checksum="B05D92BCF38A59F0" modified="2017-01-18" version="1">MYSSLSSVFEELEQESGRLKNIACACQFFHEVLDFSPAADTDNKIRDLTMVTYLMVNRLGPDYIPGLELGLGETLLLKALAQSTGRELPQLRKEYHVEGDIGVVAQKSRSKQRVMFKPKRLSVLEVWKGLNEIAKSEGTNSQSKKIGVINKMLTACEGTEAKFLMRSLEGKLRINFGEKSIIVALSKAIVEWQLKQQNEAITTELIVKAEEQFKEAFCQLPNYEILIKIALNEGILNMVEHSGLSPGIPLKPMLAKPTKSVSEIFDTLEGKRFTCEYKYDGERAQLHLSEDGKMHVYSRNSENMTERYPDLIPVVNELKLQNPKIKTLILDCECVAWDNETKRILPFQILSTRKRKDVQEKDIKVRVCLFAFDILYYNGESLIQKPLAERREFLYDNLKDIEGKFTFATKMDTDNVEQITKFLDQSVHDACEGLMIKTLDGKESYYEPSKRSRNWLKLKKDYLEGVGDSLDLVVVGGYIGKGKRTGWYGGFLLASYNQDTGDFETVCKIGTGFSEELLQKLHEILKPTQIEKAKPSYVYDSSNSNASPDVWFEPTILFEVKVADLTTSPVYKCGITYFGDGEKGISLRFPRFMQIRNDKSPEDATSSDQIVEMYSSQANLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGB8</accession>
    <name>A0A1E3NGB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_17678</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the second and fifth step in the 'de novo' purine biosynthesis pathway; contains phosphoribosylamine--glycine ligase (GARS) and phosphoribosylformylglycinamidine cyclo-ligase (AIRS) activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the C-terminal section; belongs to the AIR synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the GARS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016303.1">
        <property type="nucleotide sequence ID" value="XM_019161691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGB8"/>
    <dbReference type="STRING" id="763406.A0A1E3NGB8"/>
    <dbReference type="GeneID" id="30178378"/>
    <dbReference type="OrthoDB" id="2018833at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000006">
        <property type="entry name" value="Phosphoribosylamine--glycine ligase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000006">
        <property type="entry name" value="phosphoribosylamine--glycine ligase, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000001">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000018">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.600.10:FF:000001">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000007">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="115"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ45190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="793" mass="84344" checksum="C0D2580511E2DD1B" modified="2017-01-18" version="1">MAGKINVLVVGNGGREHALAWKLSQSPLAGEIFVAPGNGGTETMKNVTSVPIGSSPEHFPELVKFAVEQSVALVVPGPEQPLVDGISDAFKAVGIPVFGPSASAARMEGSKAFSKDFMKKCDIPTASYENFTDYEKAKAYVLSAKTRLVIKASGIAAGKGVLIPETTEEALDAIKEIMVDRAFGDAGNEVVIEEYLEGDELSILCVSDGYSYVYFPPAQDHKRIGDGDTGLNTGGMGAYAPATIATRSLLSKIETTVLKPSIDGMRKEGYPFVGCLFVGIMVTPSGEPKVLEYNVRFGDPETQTVLPLLKSDLLELFLATVEHRLDSVAFEIYSGKSATTVVMAAGGYPESYKKGDEITIDSASLADDTFIFQAGTKLSNGKVVTAGGRVIAATAIADSLEDAVKKAYVGVDHVSFNGKYNRKDIAYRAFKDAGKSKKISVTYEDAGVSVDAGNQLVENIKKMVKATARPGADSEIGGFGGLFELKKAGFDDIDETILVAATDGVGTKLRVAQIMDIHNTVGIDLVAMNVNDLVVQGAEPLMFLDYFATGHLDINVAAQFVEGVANGCKLAGCALVGGETSEMPGMYEAGHYDTNGTAVGAVLKKEILPKFDEMKAGDVLLGLASDGVHSNGFSLIRKIISSTEYEYTDKAPWNPSRTIGEEVLVPTRIYVKQLLPSTKQNLILGLSHITGGGLVENVPRALPKHLAATIDISRWEVPAIFKWLGKQGGVPIEDILKTLNLGVGMVVIVKAENVEQVTANLEHAGETVYRIGELVQRKDGAPGCVVHNAENLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGC0</accession>
    <name>A0A1E3NGC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016282.1">
        <property type="nucleotide sequence ID" value="XM_019164629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGC0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGC0"/>
    <dbReference type="GeneID" id="30181316"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF30">
        <property type="entry name" value="PHOSPHORYLASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="635"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="680" mass="75480" checksum="A9C1BCAC6260222F" modified="2017-01-18" version="1">MVPQSSRHLSDYRPQTVLGYGKFSTVIRAERSPSPIDSDSGSSNSGGSSSVAIKIITKSRKINRINKILPISSSATNYDTEIDIIRRLSPYDHRNILAYYALFSTDATVYIVQELAPGGELNPANFKHSPSRTNQDGPASTSIEVAMTAKLLDMLSAIDFLHSLNIIHRDIKPSNFLVFTNGTVKLADFDTCYTLTDDLNENKVQLYSRLIGTPLFLPPELLTQSTTKVLEAQPPQQPEAARKSKFGVKHNFAKKTEPFLKLFGRSASASSSSSSSSPYTTVPSFPLAQNSTASPASYNPFMFDLWSLGVTLHYLFYSAYPFYADNEFTLLHKIATAAPDTPPLSELSFTVNPSFPLINIIDMLLIKQPIKRWSIPTVLQELDKWDGTSHRLAKPARNWNAAPISIQRTKSKDLCISDLSEPPMDENQFNALPNTDSVKPATQQFKFQLPIFMSPKDVRSSHWAEHDKLINLKGAAQNRSRPGNEEGGGNNSSSSLPLHPSQTTKLHMHSTKPKTGTSNHPLKPRMDSNSLLRREFSPNEKLNKNPVPSSDDWKTANDYAIIMKKLEGMGTEQADSHLDFLRPGSISALDADTDKRSSVHTLPANFNLKSVKGSGRGLKHSEMMNFKKFMKVDSNEGNAENAGNGSQNNNLNYKPSYATLDNQMANDYKVYTMDDYLDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGC4</accession>
    <name>A0A1E3NGC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methionine aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_18032</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with zinc, cobalt, manganese or divalent iron ions. Has high activity with zinc; zinc cofactor is transferred into the active site region by the ZNG1 zinc chaperone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Associates with the 60S ribosomal subunit of the 80S translational complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="11"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015731.1">
        <property type="nucleotide sequence ID" value="XM_019161855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGC4"/>
    <dbReference type="STRING" id="763406.A0A1E3NGC4"/>
    <dbReference type="GeneID" id="30178542"/>
    <dbReference type="OrthoDB" id="3209743at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000010">
        <property type="entry name" value="Methionine aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031615">
        <property type="entry name" value="Zfn-C6H2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF7">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15801">
        <property type="entry name" value="zf-C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52013">
        <property type="entry name" value="ZF_C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C6H2-type" evidence="12">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>N-terminal L-methionine residue</name>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>N-terminal L-methionine residue</name>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="374" mass="40986" checksum="DD8930D960DD9117" modified="2017-01-18" version="1">MSAPATQRHCAAPNCGKVTTSNLKCPICLKAGLHEYFCNSTCFRGSFSQHKAIHAKAGVDSYNPFPEYDYRGTVKPAYPLTPTRAVPESIKRPDYALNGEPISELKNDRTNKIRVLNADEIEKMRHACKLGREVLDLAGAAVKPGVTTDEIDRIVHEATIERGAYPSPLNYFNYPKAVCTSVNEVICHGIPDKRKLEDGDIVNIDVSLYKDGFHADLNETYYVGQKARTNPDLVRLVETTRESLNNAIAAVKPGTPVREFGNIIQKHADANNVSVVRTYCGHGVGELFHCQPDIPHYAKNKAIGTCKPGICFTIEPMLNLGSYRDVMWPDGWTSTTADGKASAQFEHTLLVTEDGVEVLTARTASSPGGPVARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGD4</accession>
    <name>A0A1E3NGD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_74343</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015741.1">
        <property type="nucleotide sequence ID" value="XM_019164741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGD4"/>
    <dbReference type="STRING" id="763406.A0A1E3NGD4"/>
    <dbReference type="GeneID" id="30181428"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="59041" checksum="DB27F5E5F2BB16B7" modified="2017-01-18" version="1">MNVDKIAIIGSGPSGLVVLNEFLHTAKDGSSSINSFRTSENKIPVDPAFREIVVFEQNGSIGGTWNYSESTDPAFPADLESYSTPENLRPSLDPPFEKELLHTSVEEPLLRSIAPGVMKSRALWNKSGVYDDLFTNVSDRLMRFSSGYDIDSGADMSANPYAPFITHKDVLNYLGKFADANDLQRHVRLHTSVEKVYKKGNKWTLVVVQLDTANGIEKWYTEQFDAVLIATGRFNIPFIPYVENMDRFIVNHPDCVSHTKAYRNTDAYENKKVLLVGSSISAVDLLQYMIPKCKEVWLSFNSPKVTVQPAREAAPGQWMRDILNDESAGFHRCARIKRFTKDDGVEFEDGQVVHGFDKILFATGYHLFYPFLDIPENRDSNYIRVYSGKKGQSNYALTKADNAYLYTFTVGEPTLCYIGIAHNPLLFLTAEASAIAVAGVWSGGKHLPSLTEQKFWCDQKLECQADGLQMLDEHQFQPFARELYKYAPKNRIDLLQQVHDDEVDESRNILREIFYKISRASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGK0</accession>
    <name>A0A1E3NGK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74404</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in ribosomal function.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015811.1">
        <property type="nucleotide sequence ID" value="XM_019164761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGK0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGK0"/>
    <dbReference type="GeneID" id="30181448"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17230">
        <property type="entry name" value="TGS_DRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000740">
        <property type="entry name" value="Putative GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="64"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="290"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="40623" checksum="8D564171CBC7EE6A" modified="2017-01-18" version="1">MTTVEKIKSIEEEMARTQKNKATEKHLGMLKAKLAKLRADLLKDAAASGGGAGVGFDVRKSGVASIGFVGFPSVGKSTLLTKLTGTTSEVAAYEFTTLTTVPGVIKYRGAKIQMLDLPGIIEGAKDGRGRGRQVIAVARSCDLIFIVLDVNKPLRHKQIIEHELEGFGIRLNKTPPDIIVKKKEKGGISITSTVALTKIDDDEVKAVLNEYRINSCEVSFRQDATVDDLIDVLEAHNRKYIPAVYVLNKIDSFSIEELELLYRIPNAVPISSGNEWNLDELLEVMWDRLNLVRVYTKPKGRLPDFSDPVVLKGDKSTVEEFCMKIHKSLVDDFKSALVFGTSVKHQNQYVGLSHELQDEDVITILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGR9</accession>
    <name>A0A1E3NGR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_36478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016463.1">
        <property type="nucleotide sequence ID" value="XM_019163009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGR9"/>
    <dbReference type="STRING" id="763406.A0A1E3NGR9"/>
    <dbReference type="GeneID" id="30179696"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677">
        <property type="entry name" value="YALI0B19030P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="703"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1166" mass="133726" checksum="E2DC63BEA6DBCA60" modified="2017-01-18" version="1" fragment="single">PSVALSARRPATATVRTQQQQQQQQQPQMIMSEEEQLLISNLQETYKSILKLEVETQQGCADVNQKLLENNVGSELNNELWIVYKHVVQLLDNYYDFLLYALSPASTKTGRPLVSNYRILKRMWVYGVVAFLEVLKNVVAIFVEHEVCACFVSYSFNIISCLTDPELGIECWWAEKLGDLSRMAIALYPARYMDWKTSSVYWYQEAIKTQFGHGKIYYHMCSVENDNLEALMLIGKGVNCKDPFVPTQQYLRMIVDNVCSQRNMLTAVEMAMIDFVKIHKILLLPTYSMNEEMINIVSHYSSNFGVDNTNIDFFQIRPDCTTSDINDKVTFWFQKGANFALSNIYHLMGFGLSSNPFAKLFDLPDALKERKDKKDRRDKRRRSRSDSASILSAGLSSADPNDPEFLVNSSASHLNEKYWFLLLEHINKGVIELSMKMLKQYLSGPVQTSTPHAIVWLYFLISVCNSIKLYPASKPMFVKLLQAIFPWKVLVLYLNDILCIVRSVPELRSRYKTLLCRTASGNSSRELFDYFGEEETLWEVWKCWGSLWFDRVCPKGDYSTVFETGIKSNIFDTPLSGAIYNSHEDGERYIRILLLGGYLCDFFPEFGLKKCDNIFKFEFQSDAVSINPSNMTYFTYFIRDSRLQPLLKFSGYVESNSEFKKNDSPKLPMWDHNSYGFMTDFINNQYPAVLAHRSSLELLSFGQTEEDNEYEEDSEEDFDENENSDDQQLYAENVALAIRADGTTASHINETREFEQNENWRLNSDELILQSPLRENGLIESYKEFCPDYKIPKETVEGELAALAALIEATPGFDKNMPFDASKHIIFKPEYYKTTKTFTLDELNIDKTHIAPINTFGAAFPFKLLSQEAVNMLIWEAVQKKVIENHGRLPNLATDNTRLDFHVGGHFKYSPFTTALARSEELAEIVSTFVGHKMKPVWETDLVQLNFSLATNDPQEQLENFPQSQEEIDAILAKQDASDGQNIPSSLGVHYDSISVPLVIMLDLPEEAQGGQTTILTGNETVTRVPDPPIGSGTLIQGRVLRHLASKPVTNHNRISFVISYATGVEGELDNCVTTSLKPSVLPKNEYNKFYRDWVEFKFKKLEDHLRVVREGVVADYEEGKGFDQEHFVDKCLNIEKYFRGIWEEMECVENKPYPPPCFKIPYSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGT0</accession>
    <name>A0A1E3NGT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleolar protein Dnt1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016473.1">
        <property type="nucleotide sequence ID" value="XM_019164685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGT0"/>
    <dbReference type="GeneID" id="30181372"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018844">
        <property type="entry name" value="Dnt1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043185">
        <property type="entry name" value="Net1/Tof2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28196">
        <property type="entry name" value="NUCLEOLAR PROTEIN NET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28196:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN NET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10407">
        <property type="entry name" value="Cytokin_check_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar protein Dnt1-like N-terminal" evidence="2">
        <location>
            <begin position="118"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="914"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="791"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="914"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1013"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1082"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1098"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1186"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1224"/>
            <end position="1234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1245" mass="134880" checksum="C4945C9EC415ECEB" modified="2017-01-18" version="1">MFKLQVLIIPQSSLSTVESSSCGTANTGSDNAGDADDIDGNNLNNSKSNNGGKNSIFRTYSSSPGPGSERALNAISTPIPASQFNLHRLQSYNPNSSFAAYPTSFVSLPSHQTQNVRTKKFLHITYSSNRLETIAQEISQRFLKLYPNEEPLHILNIQDYEECDLDPDYTAEMVFDNNNICRVIVANEFNESDDAIINSNGKRYLAGDLNSVMKKVRKPKTLKHSIESTSLANTSIWSFDEESRFSKPQHSSASRSSHSYHSMNKPIGMKEDANASHMGPNISLAIPEVDDTVIHPHKSEHLKIASPIVATGSPTRITSGMLNAKSQPDLSKVDYEDLNGSMYVEQEEKRASVSTKEDAMRNRLTKTPATAPVSEAIADSSLIVNADLSSSSLNVSPENVAIELKTPESNKTSLLSMTNKRSSVSGSATKKTNKKQKTQQVSNTSSPSTNAQKSPARLDQPNSGNQENGSKLAPLSETRAVPTPASNAAIQVSIPDGKTFEVTDKEDAVKEKAAKEKGEKTEIAKAAKEKAAKEKAEKAEKAKIAKEKAAKDRAEKAEKAKAAKEKAAKEKAEKAERVKAEKEKAAKEKAEKAEKVKAEKEKIAKEKAEKAKAAKEKAEKEKAEKEKAEKEKAEKEKAKKEKAEKEKAEKEKAEKEKAEKEKAEKEKAEKEKAEKEKTANEKAAKEKAAKEKAAKEKAAKEKAAKEKVAKEKAEKEKAEKEKAEKEKAEKEKAEKEKAEKEKAEKEKAEKEKAEKEKAEKEKAAKEKAEKEKAEKEKAEKIKLAKEKEQSGENSSNIKLQVTSDKPPKRSRRVSRNNDVIVIDGTPEADKGARILKKPSNAVAADVDSDNILTDTEDDEDISKIRILTRNDTPEDEKLSHPAKAIKVTKLSVSAAKPRIPNISLASDINEMSINTSNGLKASGGSTPFNISEDSATASSEGSPANKKRNIKKQEESSPGEIISKIIMKENAKIINGAARHDEISGDIDMSNILPQKNRVAKPVSAKSTKNATGKTSSDTDSSSGTSSGSGSSSKSSSESSSGSDSSDESSDDESSRPKIVPAPKIAMKASNLSQGKREPSKKTASITNNVNSPEILTPSPPKPAPYVKPAPYVKPPTLAKAQSSTIAELSKRSSLPRLSAFLDKKLPEVRMQKNQSSKTVTTKTKSSDSDSTGSSDDSSSSSSSDSDSDSDTASDSSDSDSEDGDAVKAKFINARTANQFVSTKKSKTKTKKNKGFSALMKDSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGW5</accession>
    <name>A0A1E3NGW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sec1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016484.1">
        <property type="nucleotide sequence ID" value="XM_019161773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGW5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGW5"/>
    <dbReference type="GeneID" id="30178460"/>
    <dbReference type="OrthoDB" id="10262287at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="732" mass="82611" checksum="6F50E410C3FFB2F8" modified="2017-01-18" version="1">MLTPLDDLVSIFSSVSTYQTGKNTLVLDRGVSSQLNFLTENHFSRFSSQANIDKILWYDADTISEQIQNTIDPTSPHAATSLVFLFNLHSKENFLKLDSFASCLRDTILPLNANFNVHLIVAHPSLDKSVEKYLSTIGVLGDLTSLQLWNSLLYFEISPDIYSLESGPDAAFSQLFLDNSPLPLELLAKLLLDLYISSDYKLRITNIYLKGEKSHQFWKIYERILDSHLQTLSDNQRKIIDDIDETIFMDLHSFYNNAVDFICLDRSNDIVSLLLTQLSYSGLCNEIFNPSTQLEMIQFKPSDSDEVMKFKLDDSSDDIYPQIKYLNFSHVGPVLNHRAKQLQLEFDRRKKLKDLDEMKSFVNELNSLKNMQSWVQKHTNLAGFIIHSFKKDALDLTSGVPLLDDLSIEPFESRNLGNLSLDSYYAQLIELQQDIVSNQINTAQILARISDLLINFNSSLSDATKLVVLTSIVKNGIKESDFQTLHLEMVNKFGTLQVTPILLNLQKLKILQIKNNNQQLAFWSFSDNSLLPSKSQSILNFASLSKSLNLLPLHGDEDRSDGLFNDLAGRYTDADFGYPGYVPVFARLIQSIYDRSFLNPPNSAVENRKPTQQQIDSRRAIKYGWSNVDLDQLSGPTKQEFLVPESKRKLFNAIIPPKISELKVSNSQPNAKLSAHFSTVVICAVGGITGSELAAIKYVLSSNSFTKNKRIIILTTGMINGTQFINSMSNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHD2</accession>
    <name>A0A1E3NHD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_23238</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016650.1">
        <property type="nucleotide sequence ID" value="XM_019162302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHD2"/>
    <dbReference type="GeneID" id="30178989"/>
    <dbReference type="OrthoDB" id="2874149at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001499">
        <property type="entry name" value="GPCR_STE3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097:SF1">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02076">
        <property type="entry name" value="STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00899">
        <property type="entry name" value="GPCRSTE3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33776" checksum="0589439DECF03774" modified="2017-01-18" version="1" fragment="single">MVNLQATLISLQAICFILNLPPSIWHIMNKNIPALTLLIYVEILMMDGFISAIVWGGPDYVNAWNGKVWCDIMVRIQLAASVGISSSISCVSFNLLMIFLTNRMTTFWFGNKWVKPCCEVFFSIVFPFIISGVAYLAQSFRYLISRYSGCSPPLVTDSISVVTFYLWIFVWNFISMAISLATLILFFKKRRAAKDILVCTNSGLSVKRFIRLLTYCILVVCSSIVFSAVLGSILVIKKGVFYEKEIAQRRKWGTYIVASRNSESDINTWVLITISFVSFFLFGVGEDAAKMYCTIVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHF1</accession>
    <name>A0A1E3NHF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L39</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16880</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016688.1">
        <property type="nucleotide sequence ID" value="XM_019161378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHF1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHF1"/>
    <dbReference type="GeneID" id="30178065"/>
    <dbReference type="OrthoDB" id="6332053at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970">
        <property type="entry name" value="RIBOSOMAL PROTEIN L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="51" mass="6298" checksum="A11A717EEB4C6123" modified="2017-01-18" version="1">MPSQKTFRVKQKLAKAQNQNRPLPQWIRLRTNNKIRYNAKRRHWKRTKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHT6</accession>
    <name>A0A1E3NHT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_35807</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016006.1">
        <property type="nucleotide sequence ID" value="XM_019162966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHT6"/>
    <dbReference type="STRING" id="763406.A0A1E3NHT6"/>
    <dbReference type="GeneID" id="30179653"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12261">
        <property type="entry name" value="RRM1_3_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF8">
        <property type="entry name" value="RNA-BINDING PROTEIN MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="146"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="237"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="332" mass="37241" checksum="6FE6C6702CABF5C7" modified="2017-01-18" version="1" fragment="single">MLTPVSSLPLSDYIQNEINNNGASRAICLSNLPYNLSEKILITELSKFGALQSVEYNVDKNAAFVYFTSIFNAIKCFDQLPLSNSILSDSKIFYSNDGNSVNIPAFSASNSFMNTTFANKSELDLFKENRPNNMLTYSRTSTSLSSPLYLGGLPSGTTSEDVCNKIRGGILENIKLLSDRKAAFLTFVYHHDAANFIARTFKESLFINSKCAKVGWGRNSGNLNPEIQLAVDNGACRNLYIGVNEELEDQDDYELIPDTLRRDFSIFGEIEQINYFKNGSCAFINFTNILSCIKAVNDFQSERSKKLHNSFDNRYTKFRISYGKDRCGNPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHV0</accession>
    <name>A0A1E3NHV0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_143896</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016820.1">
        <property type="nucleotide sequence ID" value="XM_019160349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHV0"/>
    <dbReference type="GeneID" id="30177036"/>
    <dbReference type="OrthoDB" id="3999883at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="249" mass="28203" checksum="A4BC60A998858B4D" modified="2017-01-18" version="1">MPVELLMRPIMRPLVLVKSILISPHRRSSRYIPHYIDLDESKCSEYAYRKRFGTGSKIWDVYDTKTEGSGPAGPTSQNESLFWFSRSRAVKGAYKMYSAQIRHTGPKGEDEPVATCRAGLRSNVLLIRAPQAPVAELGWHPISHKVDALGSYRMFTLADGNTYQWTTEGKFLEKVKNVGEKESEVRERVARVAPAGSVGFNLIVDDTKIPREIALCSALISWVDQWNTNLSLGGIYYARQPFHARWKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI28</accession>
    <name>A0A1E3NI28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17073</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016918.1">
        <property type="nucleotide sequence ID" value="XM_019161454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI28"/>
    <dbReference type="STRING" id="763406.A0A1E3NI28"/>
    <dbReference type="GeneID" id="30178141"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="31"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="367" mass="40036" checksum="79199F4D32422925" modified="2017-01-18" version="1">MAAVIPELPPIVNNSFKVKAVTYHSYKTPLTTKEVEIPVTAGSIVKPTEVLLQIKATSINPIDCIFKGMSYGFLGPKDKIIGGDFSGIVVKAGAETKFKPGDKIFGDVLSVTRRGSSSEYIIFEPKVAQVCELIPEGMTFEQAASLPIVSGTAYQCLYSHRGSLEGSNVLVLGSGTSVGTYAVQFAKHYFKAAKVVATCSASSAEKSKKAGADVVVDYTKGKSVKVNQILEFVKEHGKFDIVVDAVRDEDLIPYFSHVLKPVSENGIFSQVEGSYTIDYSHVHLYQFVPSWKKFVSVVKYKLGLYDYFIDTVLLMNTKGYGDAVRTLWANRELSIPIDSVYDAYSDAQVALERVASGKAKGKVVLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI43</accession>
    <name>A0A1E3NI43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_59418</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016912.1">
        <property type="nucleotide sequence ID" value="XM_019163365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI43"/>
    <dbReference type="STRING" id="763406.A0A1E3NI43"/>
    <dbReference type="GeneID" id="30180052"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000191">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="151" mass="17083" checksum="0F24050E6BDBE8FE" modified="2017-01-18" version="1">MSSTLFDDIFNIESLDSARYDRVSRIIANSTSSQDTKITLDINHELFPVSENDTLTITLAKSLSIDDDPSSMETDELLGANGSWRPPKPDQRSLMNDYDYVMHGTVYKFEEGKGDTISVYCSFGGLLMCLEGNYRNLSSLKQENLYILMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI51</accession>
    <name>A0A1E3NI51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIF subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">TFIIF medium subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">TFIIF-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_17041</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016879.1">
        <property type="nucleotide sequence ID" value="XM_019161438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI51"/>
    <dbReference type="STRING" id="763406.A0A1E3NI51"/>
    <dbReference type="GeneID" id="30178125"/>
    <dbReference type="OrthoDB" id="26094at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="12">
        <location>
            <begin position="96"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="11">
        <location>
            <begin position="399"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="246"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="469"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="477"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="507" mass="57971" checksum="29948E316487849D" modified="2017-01-18" version="1">MSASPDVNAVSSDAKTGSLSGNRNGSAVQAAANSKDKTNGGFSESTNAEESEGRELSEEVAYDQDDLPIPQGIVSVNENQLDQDESLDLNLQNASNKIWLVKLTPQLADIWKSDHFLEGQLLGNIKIPKYSTNDPNNTLSTATAAVPANGSINGKKDGKILLELNDELPEHKNLDKEYELRLIKQVVENEYAFSESQFEQFRQRLMYRTQVVNLPNQPRMKTLNKEALNHEAKWTRKIRNYKKEAQYREMKSRDPNYRSKRNESGMGTTYIEDPEGGDANKNNKRGGRKNAKFEKYDKFRKFVPYAKTIPKKTEMVGKIVQECQVIPTKLLSDNKLRMLEQRRRLNQMVKRKTINYIKSERVGVMHGRTTPNIQTGSTITLSKELAQKKEAAKQDGRAARMDRDVLMNLLFKLFNQYEYWTMKGLREKTNQPEAYLKECLESIAVMERKGPYALKWTLKDEYKKSIDVEKKVKNGSADEEDEDNNKDNEDNDDDELEDVEMEDIAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIX6</accession>
    <name>A0A1E3NIX6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_64437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017205.1">
        <property type="nucleotide sequence ID" value="XM_019163480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIX6"/>
    <dbReference type="GeneID" id="30180167"/>
    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031349">
        <property type="entry name" value="Tfb6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781:SF1">
        <property type="entry name" value="ADR380WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781">
        <property type="entry name" value="TFIIH COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17110">
        <property type="entry name" value="TFB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="405" mass="44546" checksum="DDD2BF45758C60D9" modified="2017-01-18" version="1">MDRFKIPDSPPLPKQETNAPETRIPAQLGSELDLGDDDDDDDEGDVRVKREEDVDMADELPPGPGPATVFSTGRAPQPINNFEQFEHTDSLPATPEDLPGSGAGSCAVPQDPNYVTPMMDFSDPVRPPLDLLNPFGANNTLSSSDPIPITLSGSGSSRRLSDLQQQKVSEYIDDRLLGIQRGFVKYLSSKEEADLEGLAWEQIAARIDEVVEFVWYALFHVTGVPAVYHSNVLADECIRAIFDSRFPEIKTRLDASIRPCQHLLVRPSADAPLPALALPATVDTPAHVSFLIKLSGDLIDYIVKYDFATFTDWIVLLRLAAKLDNVLSVLVDYSALPTTPTLVSTTDKVRLASIVQRTKIAVVELFDRFVRHLDPAQTALHQPAIDHFQGYAGETYEGLVDRTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ64</accession>
    <name>A0A1E3NJ64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017300.1">
        <property type="nucleotide sequence ID" value="XM_019164143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ64"/>
    <dbReference type="GeneID" id="30180830"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="243" mass="27573" checksum="0FE10FA217999477" modified="2017-01-18" version="1">MSDKPFSTFSHKPLNYNKIEGFCEIRWTTNLVFLSADPANVTTVQAFLDCLKYHNATDYAYYVTLQGYLNLSSLSGVNADADIDKTAYRIYNQLFGFGFYASFDLGFRRYYDSGFVNNIENVSGEDLVNEGVIMRESLYIATHVAANTSVVNQYRTVAENIGYNQTEIESLISTNASGYNTHLLRRTTYYEAYATDSDATSANRNYELFEQSTSYDHSSSYQPARLGINSCGYVTSNFYPHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJH3</accession>
    <name>A0A1E3NJH3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017373.1">
        <property type="nucleotide sequence ID" value="XM_019164165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJH3"/>
    <dbReference type="GeneID" id="30180852"/>
    <dbReference type="OrthoDB" id="21418at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645:SF1">
        <property type="entry name" value="YALI0F12243P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="263" mass="29063" checksum="809298455D11A14B" modified="2017-01-18" version="1">MDLSKLSSNLPLSSSPTDESISNLNKELSDEFKIGARSIAALYRLSNSKNSLLIARGYLDCLNEISTYLENGNISSLAELSHFITAKKHEMAPETAASTPHEEMPPRDNLVQSGIDTSFKFTVNQPTDHHFPHSRAPLSVDNHNLRYYSKPHQHQKKLKSKASAIANANTNAANAAAPSSDEEEYVNASQDDEERAGVAYFVSGKSHTSPKSYLSSGIDEHDEHDYDYHNDNTSFEEATGSANTLKRKTLDNASLYKKQKLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJI0</accession>
    <name>A0A1E3NJI0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017384.1">
        <property type="nucleotide sequence ID" value="XM_019164170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJI0"/>
    <dbReference type="GeneID" id="30180857"/>
    <dbReference type="OrthoDB" id="5397701at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37331">
        <property type="entry name" value="YALI0F11671P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37331:SF1">
        <property type="entry name" value="YALI0F11671P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="389" mass="44531" checksum="FE3475D65BBBD11E" modified="2017-01-18" version="1">MSATMNRILVFGPENYDKIGFVRRLFQVGEIGEEQITRGDTPRDKQGGLIIEDLPLQTKYYKTDVGVFIDEPETNDPQNYVQWIEELSSDEMSELREQIQLLFVLFPGSDGYEEFISGPLDKLNALLDAEHCETHKESLQWDGQILAMDTADASLLAEAQNAIQCVVWRNIELHDDDHDVFGALTTRQHGNGHHQDLARDMDLELRHFEQDLKMNRIAKAAYSVNRSLVNTIKYAKQDQLYLHQFNAGLTAVSFSPDHNKTPIGTVQTSGAEDKNLENLVLSVRNFKENKDFFPVLHKAFFENVYDDQTYIFDAMNYPGSFMAIGDYKVVLDYMNQRPELANTLGFVHVDPEGVMERGSYEKNDMYTLCNIDGIVTLSENMNEQLQKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK67</accession>
    <name>A0A1E3NK67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_58821</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017647.1">
        <property type="nucleotide sequence ID" value="XM_019163322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK67"/>
    <dbReference type="STRING" id="763406.A0A1E3NK67"/>
    <dbReference type="GeneID" id="30180009"/>
    <dbReference type="OrthoDB" id="2110361at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001158">
        <property type="term" value="P:positive regulation of L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="869" mass="98105" checksum="28D2BCD2E9C511A1" modified="2017-01-18" version="1">MACTNCRQRHIKCDGMGEPCTRCLNLKQNCIYLEAERKVVISVKYLDKLQNLISKLKVENANLRTLKKTTKKEPMPESSQSKTASSYSSDDEGDEPLELVLEHDSGNAPATNAATTIHKRQDTEQPVANLNQKETAEPHFNKEFTSENHILDDHGLATHESAGQHDNKSSSSKAHDSSTGNEKKNQEVIPPFLEQYGRLIHSRTGERLYIGSSSMTLFGLEIGRLVAPMLSNKATEGPGAADASPVSESPQNKAETETILEKEGNAYRIMLGKTHSRPGINVNFTLPSYSYAMLLVDSFISYNDGCFYFFNEGLVKENLRRIYNDETDTGFKLDYGIESLNTSPQNESTLETIWFCKLLLVFATGEINPQNPKKKNKPNYTGTKLPGSGFFNQASELFTGLFASGSIDNCIREGGIEVLLLYAFYLQVADCTAASYFYFGIALRASLLVGLHVDIGKETLNRFELEHRRRLWWTVYMYERMLASKAGLPLSLTDEDISTELPSDFDMSQPAPGCENYIFPEAEFISNCVKITQINANILRKLYTRHPSSNILPVVHEMVVALLKWKKALPHDINCDYSQPELTISRLVVNMMTEYFQGINLAVRPLLFHFVLLNLKSTNPKEGKYLDLSKYSSVVLALLNASFQASINTVRSLWALVPENMVAMFGYMDREYLFNSTATLVLFNATFGVYDATNEHLDHALTLFTKMRNLGNHPATLRREQILRLISILDFNGSMSGLLKKHNDNLNPPSSRTPHHTLQHTQAHHQNDHQFNLQQNPNQDVVHHHQILFMDSLASILDRPRPQHEINTGLFSPSLSSIFNQIDDIPEFEQLSSTSNDMELWKDVTNQAAWLQNDMGDEFEFLLDKSDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKA8</accession>
    <name>A0A1E3NKA8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit</fullName>
            <ecNumber evidence="5">2.4.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GlcNAc-PI synthesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_72639</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="5"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017695.1">
        <property type="nucleotide sequence ID" value="XM_019164258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKA8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKA8"/>
    <dbReference type="GeneID" id="30180945"/>
    <dbReference type="OrthoDB" id="734129at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03796">
        <property type="entry name" value="GT4_PIG-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000053">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000026">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039507">
        <property type="entry name" value="PIG-A/GPI3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="14">
        <location>
            <begin position="51"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="204"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="446" mass="50119" checksum="D82AAF0DEA6CB0C0" modified="2017-01-18" version="1">MGDCKKERQKHYNIALVSDFFYPQPGGVELHIYHLAQNLIARNHSVVIITHAYGDRQGIRILTNGLKVYYVPHLVFHRQSTFPSVFSAFPILRQIFIRESIEIVHGHGSCSSLCHEGIVHANTMGLKTVFTDHSLFGFADVASIIQNKLLKFTLTDIGHVICVSHTCKENTVIRASLDPSIVSVIPNAVVHEDFKPALIRPSLNKITVVVISRLFQNKGADLLTAIIPRLCSDRPDIRFLIAGDGPKFIDLEQMRERYQLQDRVELVGSIRHEQVRDIMVQGQIYLHPSLTEAFGTVLVEAASCGLLVVTTNVGGIPEVLPSKMTVFADPDEDSLVEAVNSAMIKLKENTVDTSSFHSEVKKMYGWADVARRTEKVYDDVFKRENTLLINRLIKYYNSGSWAGILYVLCVVTDFLILGILEILYPKDKVDLARKWPRRSASSHLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKD0</accession>
    <name>A0A1E3NKD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_72636</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="10"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017692.1">
        <property type="nucleotide sequence ID" value="XM_019164255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKD0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKD0"/>
    <dbReference type="GeneID" id="30180942"/>
    <dbReference type="OrthoDB" id="10266385at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000006">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="9 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="11">
        <location>
            <begin position="39"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="370" mass="40498" checksum="1E7E12848A778D05" modified="2017-01-18" version="1">MLSNNNVWKRAAAQSKRFLHSSVGRRHELTRVENGVEVMTVRDALNLALQEELDRDPDVFLMGEEVAQYNGAYKISRGLLDKFGPKRIIDTPITEMGFTGVCVGAAFSGLKPVCEFMTFNFAMQSIDQIINSAAKTYYMSGGQMPVNITFRGPNGAAAGVAAQHSQDFAPWYSAIPGLKVISPFSAADSKGLLKAAIRDPNPVVFLENELLYGESFPVNDEILSPDYVLPIGKAKIEREGKDVSVISHSRNLIFCLEAAKVVKEKHGVDVEVINLRSIKPLDLDTIFASIKKTNHAITVEAGFPQCGVGSEICAQVMESEAFDYLDAPMERVTGCEVPTPYAKTLEDFAFPDTPTVVRGIEKVLSLGEWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKD8</accession>
    <name>A0A1E3NKD8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_132459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017725.1">
        <property type="nucleotide sequence ID" value="XM_019160034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKD8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKD8"/>
    <dbReference type="GeneID" id="30176721"/>
    <dbReference type="OrthoDB" id="5378975at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031355">
        <property type="entry name" value="YBL010C/LAA2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17104">
        <property type="entry name" value="YBL010C_LAA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="307" mass="34374" checksum="4A8A1D8338942220" modified="2017-01-18" version="1">MSSEKAEEGVLSNGQKLETAEPAIDRAVADNEVIVGNEDASDDDEFGDFEEVDQIEPAEEKLDVSTELKKELSLYDGDYVKNEENITQLLGNVFNEIADTKTSFGELESDTAGTASPTDQRFVFNERASKVFDQLVSDNDGLTPHIIWRKSMVFKQLMLNLNIPIENTNNISGKVPQTGSTSSTTEIRDLYDRESTSNVNSELENLLAQVPDFASLGIDKKSEVFADKITNTTRIITEAHHAIREHSENNEGYLVELVAVKKNLLNLLSVWDERMGDIREDNELFSSYVENLIGNTQKFRRVSKIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKE3</accession>
    <name>A0A1E3NKE3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_145542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016916.1">
        <property type="nucleotide sequence ID" value="XM_019160429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKE3"/>
    <dbReference type="GeneID" id="30177116"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="109" mass="11862" checksum="E00673F3744FBCD2" modified="2017-01-18" version="1">MLSRTKQSNRAYCTLHYSFIYGFSSACPCWGKIPLLQVQLSVRRAASKHLLGRPPSLPLPLSLWLQTQSSSNGSSQLPTICPSIDTVIVIVSIHRLPSPTPTSPPSSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKR4</accession>
    <name>A0A1E3NKR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16513</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017789.1">
        <property type="nucleotide sequence ID" value="XM_019161232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKR4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKR4"/>
    <dbReference type="GeneID" id="30177919"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="5">
        <location>
            <begin position="67"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="226" mass="25346" checksum="6E64F60509F92733" modified="2017-01-18" version="1">MSDVEEQQQFEEEIVSEPVEIQYVELATPIPKDVQEGSAEVKLFNKWTFEDIVVKDISLTDYIQVRQPVFVSHTAGRYATKRFRKAQCPIIERLTNSLMMNGRNNGKKLKAVRIIKHAMEIIHVLTDQNPLQVIVDAIVNTGAREDTTRIGSAGAVRRQAVDVSPLRRVNQSIALLTIGAREAAFRNIKTIAECLAEELVNAAKGSSTSYAIKKKDELERVAKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL39</accession>
    <name>A0A1E3NL39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PQ-loop-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_25062</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the laat-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017978.1">
        <property type="nucleotide sequence ID" value="XM_019162379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL39"/>
    <dbReference type="GeneID" id="30179066"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000011">
        <property type="entry name" value="PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF35">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER YPQ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="32869" checksum="078F644B77650F79" modified="2017-01-18" version="1" fragment="single">MAPPPTPFIDAQAVSGITGSISIACWIIVFAPQIYQNFARRSADGLSLLFVVLWLLGDIFNVIGAVLQGVLPTMIILAIYYTLADIVLLWQCLYYRHGSGKKVDPIHLSPANPLNSEAEPLLESVMSNEHHHHHHHHRHEDGAANGSNEADEDSRSSCSSASSVAGSDLTYNLIMVSLVIIAGLGGWYFGGSGKGSDDPPKGDDGLEFNPLAQTFGWLCAILYLGSRIPQILLNFERKSCDGISFLFFLFACLGNVTYVVSILAVSTGRNYLLVNSSWLIGSFGTLAEDFIIFCQFFMYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL55</accession>
    <name>A0A1E3NL55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16665</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017963.1">
        <property type="nucleotide sequence ID" value="XM_019161298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL55"/>
    <dbReference type="STRING" id="763406.A0A1E3NL55"/>
    <dbReference type="GeneID" id="30177985"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000008">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF9">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT TAURINE DIOXYGENASE (AFU_ORTHOLOGUE AFUA_3G01010)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="135"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="443" mass="50225" checksum="85DF97DBEDD903C6" modified="2017-01-18" version="1">MSAATTTTITEQLSNPERETVKIVNDAPIDEQIEELIRNNNQINITRTRHKKIALQPGIDKSGLAILPESTRKRLEDDDIDIAKGYPEVPERNKIPIFVDQAHAIRDYDVPFVDRGKGADPEKKALFGAAKEVINLTKNVGTEIVGLQLEDLTNQQKDELALLIAERVVVFFRDQKLSPTAQHKLGDYYGNIEVHAQTPQVPGLPGTTVIWNDYQVSQGMALNFSNSNLGNWDSTKYLSVGNQGWHTDLVHERQPTGYTHLHLDAIPEVGGDTIWSSGYGAYDKLSDEFKKFLDGKEAVYVSAHQYLDRDDPFGGPKRIERVHPLVRTHPATGWKFLYVNRGMTKRILGVSPVESDLILNYLFDVYEKNADLQVRFKWTNTKQGYGTSAIWDNRVSQHRNVWDHEGAEPRHGTRVTSLAEKPFFDPESKSQRESLGLYLHQWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLC5</accession>
    <name>A0A1E3NLC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YMC020W-like alpha/beta hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_33914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018033.1">
        <property type="nucleotide sequence ID" value="XM_019162854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLC5"/>
    <dbReference type="GeneID" id="30179541"/>
    <dbReference type="OrthoDB" id="5598028at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058934">
        <property type="entry name" value="YMC020W-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058933">
        <property type="entry name" value="YMC020W-like_ab_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349:SF1">
        <property type="entry name" value="AER328WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26147">
        <property type="entry name" value="AB_HYDROLASE_YMC0-YMC35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YMC020W-like alpha/beta hydrolase" evidence="2">
        <location>
            <begin position="61"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="470" mass="52994" checksum="15BED815BF148B52" modified="2017-01-18" version="1">MRLDQPSRRKSSWAFWRSENAAEPSSPVQAAADATTPAGGKPPKPQKKNVPHANRPNLIVPQIEDTLPLLSTHNLLAGGVKRIKKILGYTDTRDTNLFRKEEPTIFKRVLIIGVHGFFPTKMIRPIIGEPTGTSMKFAQVGETAVLSWAKENNLNIEIQKIALEKEGKIFDRVEFFFDILKNSANDIKKADFIFVCSHSQGCPVSVILMSKLLEYGIIDENKKIGILGMAGINIGPFFGMDRSFFVRAYSTIENESLLELFQFQNFESLQSRKYIESLRNLVRHDVKLTFVGSIDDQLVPLYSAIALHVQHPNIFKAVYIDGSTNTPDFVARIIKLSCMLHNIGSTDHDVIKEISNALAGPITGGGHSRIYNDLKVYKLALDFILKTESSNIAIEQPVRYRPFDIKKLGNGANPFNLPWCARGLFFEVSKRLPSGKDEIQLVFREFEEWQPESKALKDVKYRLNGIRAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLD2</accession>
    <name>A0A1E3NLD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exocyst complex component EXO84</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17353</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017266.1">
        <property type="nucleotide sequence ID" value="XM_019161559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLD2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLD2"/>
    <dbReference type="GeneID" id="30178246"/>
    <dbReference type="OrthoDB" id="642193at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25345">
        <property type="entry name" value="PH_EXO84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="699"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="699"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="721"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="874" mass="96036" checksum="25B9CF190E1BC9F8" modified="2017-01-18" version="1">MADLNRRFSRSQWNKNGISAVPLQNGLPGNGNGNGSVYGSGNGQYPPGGYNTSGASYSYNATNSSLNVNDYGNRSNNTTVLHPNMMTSAESIHSNATNGTTMTTRRRFSMKLSQPPLDAYNTYGAPALPYNAQSQILQSSFTNASETFSATANDDGHHPPLSAIQQNNYSQISNIDNNGRSPQKAHHELLSELTTDNFDASRYVQIKLGDANAARIDDFSTYMETLNVANDDAVKYSLSESTSQVLAVSEAFRQTHEVLLSLKPKVNDLTDILSQQLEEAHEYQDKAGTSSAGPAEGNNQLDVPNSKLNRQSVMLLQNKWSNAMKKLYANIDRAHDLLPPLPSRHIIIESRRWGELNSITCNPVRPVHIVVMNDSILVASRVRDSSKYLNGGKSGSNSKKSKSSRNIATHLWMIDKITVQKCSEIKELNEVLSHNSSHSFSKGANPSSSDSESAADSTLCIKTIDGNQTFLFQTDLSTEFTRVFSAVQQARAEMSSMKRRSMRDSISKLNLAGLNNKPDSRHVSMAGISQNSNSLLEKQAEPELNACVSAIDDLLTSASLELGLQRYDECVGYLTRLDDELRHLAQIATTVGIPKSLLSSKTLTTNSKNGSQVSVLTQQIHFTYNIKMSSLKNITDQLVDALLDEISSSSSSLDSLKEYIDIFKILKREKEAAEIYLESRGRELVDCVGMVRVGGGYSGNSSHELSSTNPKGLLGNDRGLSRSSSSRAITGLTSRPTSMSNTPDPEAENETEDVSNVNLDSIGGVTGELITAYVRELSLVYMGFVSRVWDEWNMLFTPQKSDPADKKGAKTGISSVRIIEWVNEYMAELKRSVQLALVDYERNGEVFMSSLQTMKVVFEGLKQRELNVDYLLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLD5</accession>
    <name>A0A1E3NLD5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable metalloprotease ARX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Associated with ribosomal export complex protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_11918</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable metalloprotease involved in proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Accompanies the pre-60S particles to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018075.1">
        <property type="nucleotide sequence ID" value="XM_019159856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLD5"/>
    <dbReference type="STRING" id="763406.A0A1E3NLD5"/>
    <dbReference type="GeneID" id="30176543"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF102">
        <property type="entry name" value="METALLOPROTEASE ARX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="13">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="562" mass="61033" checksum="C8F6A098C8713565" modified="2017-01-18" version="1">MALQISPKDTDLLLKKKNVLSADVLDKYRYAGEITQTCLSYLISLINDSYHLGKHEPYSAAELCLLGDSFIQTLVNSKYKKVIEKGIAQPVTLDVNDMIVGYSPELTDESNLTFQPGDIVTINLGCQIDGYTSNLAHTIVIYPSGLTAPTGPLLGGPADAVCASYLATETVVSLLACALSPEKIPNSLLSDNSNRHITGSMIRKVVDTIAQSFNCAIVPTSKVRRIRRFLAGQAEGVVAERDFKGVVWSEIDQESDLLKRSNFQDGSASASSGNTDLSIIDQTKGRSDIQNPDKAIPTDDFVVSAGEVYTVDIKMAPIDKQEPGLVTVDSGDGRDRGAKHSIFIRDVSMSEQLKLRNSRRLLSLVDKEQSVYPFKLAYVSESFPLDSKKDLAEQLAKIEKDVKIFRFGLTECTNKQLFVPKAVLSCKFIPLREVLKVPTSTGINGFDASNPALPGMELPLPKLGLTALKLKTLLKHGRPVTVSRFLSTVALSPDAGCVRLSGGNKTFTPSWVHSDYQLQGPVAAAVGELAALVQDERFGLKIDECRVMDLSAVAANDDAMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLJ4</accession>
    <name>A0A1E3NLJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018103.1">
        <property type="nucleotide sequence ID" value="XM_019161343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLJ4"/>
    <dbReference type="GeneID" id="30178030"/>
    <dbReference type="OrthoDB" id="3995865at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="47153" checksum="EBA5CE988C39875A" modified="2017-01-18" version="1">MFTVNQLNNRYNLSEDDIRKMKTVYETIAYSFKGPLPTEDFKALEMEHYTDERGSTQFFYLTSETGEVASVTEIVPRSGNHDNSTNLLVTMVFTNPKYRRRGLIAKLINWVINFYETGVLESDETLLLSDSIRKEECRAYVDSILPQALRASKRIHWSLYSIIGDYYKQFGFVGCEDITWLEITATALAQEDKAVQGKDFTADARTERLLTAGDLKEYFFDDEYALPKVIDEHLLNCDAEESSFPGFIERIQSYVKMHESEIENSKFFETCGILITDPHNADIKTIVFICPFFFINRIVVNRVYTSAPDISTFQHQWQRASQFVHNYAATTWTTLPCLQSVPAADKVIMLADNDFVSKNGAISKTEMVEVITKNSSWTNKGIGAVLPMIRDWTQHKEQPSRLANNGHWSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLX5</accession>
    <name>A0A1E3NLX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alkaline ceramidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71242</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018243.1">
        <property type="nucleotide sequence ID" value="XM_019163868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NLX5"/>
    <dbReference type="GeneID" id="30180555"/>
    <dbReference type="OrthoDB" id="187171at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="32952" checksum="048019DFA270B85F" modified="2017-01-18" version="1">MFGFSYPTTAFEPYWGPPSATIDWCEENYLFSPYIAEIVNSLTNVGFILLATHHIYSTFKNGHGLLYIFISLGFASVGVGSFMFHSTLWYEHQLMDELPMVWVTAIPFGYIWGWNRPEPWHLLANVGTFLVTSLFTYIYIYVYRNPVFHQAFYAILNFGVIYKTVKTTQECIPDPQVRRRQYKLLILAFSLFIFGFFIWNLDNIFCKTLINWRRSYLGMPLGILLEGHGWWHVFTGLGIYYFVLYNMILTTYMRGEADQYEIVRYGFLVEVRRKRVKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLX7</accession>
    <name>A0A1E3NLX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_19664</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017456.1">
        <property type="nucleotide sequence ID" value="XM_019162134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLX7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLX7"/>
    <dbReference type="GeneID" id="30178821"/>
    <dbReference type="OrthoDB" id="5595695at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydrolase-related" evidence="3">
        <location>
            <begin position="269"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="632" mass="70088" checksum="5551C5DA93C86DD6" modified="2017-01-18" version="1" fragment="single">RDSGVPREEVFVVDKLANTWHTAAGECLDVTLQALGVDYLDLWLMHWPSPLNHHGNDPKTPRRPDGTIDFEQDWDYVKTWEAMVAVHKAAPHKVRRIGVANFSVEELERICDATGVVPDVNQVEMHPANNQQRLHAYCRAHGIQLVAYSPLGSIGSPLLSNAQLVALAHSKGATVAQLLLSWGVASGWPVIPRSQSPGRLDENLSLVDLTPADYERIEKLRQSMAAPFYRLENVSFKVDPAVGLIVEVVQRASGDCEVGENDVDLRGKFVMPGFVDAHTHIFLHSYDEKDSARQVRDEGFVERIIRATNHCKTALMCGYTTYRDLGSEGMDDADANVRDAINRGLAIGPRLFVATQVLASVNGFKTHTENTIGGTRVPAMSEACDGEDEIRRAVRRRLGVGCDVVKFYADYRKRIMRFPPAQPHPYLSSVQFPPDVPNPDVILYTQHEMDVLVAEAKNADCPVAAHCATNRAVKMAAEAGVSTLEHAFWCDDETLQVVKKHDCMIVPTLAIAERLYGSNFQFMLDKTYRAWKLGIKQACGGDTGTFNHGENIREAELFIESGIPVDETLRCLTYNGWLACGGHRCGRLFGWLGEGTAADIVALDESPFDSFSTLRKPSFVMKDGRVYKKDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM59</accession>
    <name>A0A1E3NM59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_32528</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018269.1">
        <property type="nucleotide sequence ID" value="XM_019162774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM59"/>
    <dbReference type="STRING" id="763406.A0A1E3NM59"/>
    <dbReference type="GeneID" id="30179461"/>
    <dbReference type="OrthoDB" id="1405595at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="715" mass="81290" checksum="0555CF2B4AC99883" modified="2017-01-18" version="1">MAVEISKKRKRRKGPFICPVVNCGKVFSRSDHLARHQVGHSSQRLRCDFEGCGKVFTRIDVKKKHEAIHRKKNSGQTSPQDDNNEFASSSSKEGNMVANKTSPMYSSDPHIGKNTLSLLEEVFALSPNFPNENCQTEIDNKTLYNMIHFIPELENNADFTVAKIKWFLELYWSLYHCQYPILHRPSFSTYDAPPLLLLSMIMTGSSLAKKVTPPPGFLFEDPSKLAMIIAEPLRWLVFACNEAKPPCKAWVIQSLIILETYEIISSSRAFHERAVIYNGAKIQLLRRSPILGGDPFKSYGSDTSKSTNLWNTWIESESMKRAALMSFNLDTIHAVVFGHPMNIFANQIKLSLPCPDDIWEYKNVDRNKAPYHVTETPLFLDALKSLLKGEAVNVDSFGRQIILSGLINLVLQTEQNVLQWNNFGWKTIEKSWKDDISAAIKFWKTQLPEGNCCLTLSSVYYAGATSPPLPPSLMPEDTRCKFPVYHAAQIYLRITHYDYIVFAGAPKRMNVPILPEDYAIVVRRIDKWSNSVSGKLSVINSLILLFEMLLSPEGSVDIVNYYYEPDKDPFIYRPNVIICAILSLWAYAFHVFGPESLFSSPDLRHQLKSGFIPAMEDGSYYLRRVRSELVKRTGISFSSLDHMDSAENIKAMKEFCRVLPEIRGLHHLIGLLRTLETSYLGCEWQVGREYAKLLGNCIKRSAGATHVFCNDMYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMF7</accession>
    <name>A0A1E3NMF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_111132</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018427.1">
        <property type="nucleotide sequence ID" value="XM_019159755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMF7"/>
    <dbReference type="GeneID" id="30176442"/>
    <dbReference type="OrthoDB" id="274922at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="13">
        <location>
            <begin position="18"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="91" mass="10108" checksum="DCDCEA0781E83339" modified="2017-01-18" version="1">MSFLGFGSQPQISTEQKIAAAEAELDMITTMFNQLLDSCYRKCFDKNYTSEDLTQTESLCVDRCVSKYFAANVKVSESMQTVGKSGVLAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMJ4</accession>
    <name>A0A1E3NMJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PIG-P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_26291</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018467.1">
        <property type="nucleotide sequence ID" value="XM_019162440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMJ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMJ4"/>
    <dbReference type="GeneID" id="30179127"/>
    <dbReference type="OrthoDB" id="690928at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016542">
        <property type="entry name" value="PIG-P_GPI19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008765">
        <property type="entry name" value="PIG-P_GPI19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="8">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="124" mass="14413" checksum="E1ECADF6374B8881" modified="2017-01-18" version="1" fragment="single">AEYKGFASYVISCIFLFTWICWSFMPDRVLNKMGVYYYPSRWWALAIPSYVIVLMMYMYVGIACYDVEYLTLPLDDNRNVVDDSGIVVTQLENFRAKDIDKYAYSGTSGVWDLPISTVNQILYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMY8</accession>
    <name>A0A1E3NMY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15425</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018590.1">
        <property type="nucleotide sequence ID" value="XM_019160822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMY8"/>
    <dbReference type="STRING" id="763406.A0A1E3NMY8"/>
    <dbReference type="GeneID" id="30177509"/>
    <dbReference type="OrthoDB" id="407390at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901524">
        <property type="term" value="P:regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF25">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="8">
        <location>
            <begin position="49"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="471" mass="54544" checksum="C6B852036B668330" modified="2017-01-18" version="1">MRSQKAALEELAHNINRLQPFHPGSLILNEQHFYQNSILLGWSQKNAHPVTLRHLANFGRKMTSEKILASANFVRTELPIRLSLKIKELQSLPYQVISNYHLNQVYQSYYHCFNAFRRVEKIETLEDNEDFCDFVKNMLDDHLIVLPHLMMGALEVSILKIMSQSDLDKFMSSMVRSRISRRVIMDQHTSMSKTFMESLRLQKDDDGGDNDIKPPDYIGEAFQLCSTHEHLKLSYNMIINFLQGLYPNMQMPELVINGDDLKFQFMTNHLSYIFTEILRNSLRSTIEQFIKLNGRDRGFAQMSPPPVCVEITDTKYEMTFKISDQGGGIPLEKLESVWSFGKSPELAKRYLDSFHALPGLDLPTRLPIIEHQYFQKRSEHEMKRPEKLAGMLDNIGQMEMSSSQNKNSMLKSLASRPFEYTLGVSLPMCKVYTDYWNGVLEMCSVEGYGSDVFLKLQKLGHRDERLQLDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN62</accession>
    <name>A0A1E3NN62_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15468</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018648.1">
        <property type="nucleotide sequence ID" value="XM_019160834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN62"/>
    <dbReference type="GeneID" id="30177521"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="98" mass="11202" checksum="8D1D40F7D459EFB0" modified="2017-01-18" version="1">MSQKLDDISMYGKRVVAYDDNVVSTFSTLDMLLLSPENTIFKLYLIMPEKQRCYNNDYPSFAPGCLTCTLAGPITVVLRRLLLPRSSHKLWKLIIVNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN63</accession>
    <name>A0A1E3NN63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_15474</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018655.1">
        <property type="nucleotide sequence ID" value="XM_019160839.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NN63"/>
    <dbReference type="GeneID" id="30177526"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF4">
        <property type="entry name" value="NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="5">
        <location>
            <begin position="1065"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="524"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="613"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="320"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="393"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="552"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="618"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="658"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="695"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1299" mass="147832" checksum="D488DBC416074E4D" modified="2017-01-18" version="1">MSRKRHTINIPGVMLDTIRDNTNYENDERFTKRRKVKGSVLNRKESRKQQRLEKKTNKVEKKNQHKEKFKANIKSQNNSKDRQSTAVTPKMNVKKVHIKESLADNKSSSRSTKSDGGEMKKKQKRVSFSDHDDIREIPAREEATKADIELDFEKWEQEFASDDEDSDEHSSLSADDSQDEAEENEAYNLIETLKQLKQVQRGEYSAENSENSDDSETEEQLDDEQKGVMEQLAKLKSKKANSSFKDIRIVAEDDLNDDSLSDKVLQEEEESEETSGDDTSNDGEGEGLDFEQIEIMKKLKSLKKGKPNKGRSEIKIVKEDDLDDDEISSEDIAEENEKDNEEENEEENEDEQREDEGLDDEQQSIMDQLAALKSKKGPLTSTKNAIRIVKEDDLSDDTEESEASYDYEMEQSSDEGLDEEQDQVMKQLAALKKNNKNTNSSQLKIVKEDELDDETVSSESDFDSGSRSGSDSETFDAPTTSNFKNDDSDVEFYAKKLGINPKKKLTRQDDDDLVGGLFEGLDFMDNYDVEDNQDDEEKSEKKEKKEKKKKNEKKDKKQESSRSSKPVLSEKQRKMLEKDDEEINYYAKKLGINPKKKLPKQGEEDIIGGLFDGINLDFSDEEDEEMDDSEIPSDSESVDEEDDKEELDDHARNYVPENGDDQVGSDDFDDDDELDDDDKALLKEMYGLESSGSDSDSEYDSNDSENDGPRVRENPYVAPVEPGSNEPPLPGSKYIPPALRKKMALGDNSEDSEQLKKLVRLVKGPFNKLSEPNISTVVSELNNLYNDNPRQFVNEAILKVVIQSVCIPTPMLESFLVLYSSAIVAIYKLQGVEFGAFIIQRVVEEFEKDIESESRAKQEIINITGLLGYLYTLNLVSSTLIYDIIKMKLIKNPTELKVDVLLKLIKSCGNKLRTDDSTALNSITLELSKSVKNSEKTGGKVSSRGRFLIDTITDLKNNRMKSSENENTTLMINRLKKQLGAIKNTRNLEPIKVNLDDIENIEERGKWWLVGSAWKGLDLKEGEAVEHEDSIANANEVLDDAEDDFLGEMEVNWLQLAKEYRMNTDIRRAIFISIMSAEDFMDAFMKLEKLNLKKTQKKEIPNILMHCASMESNYNPYYSFLAKKLSDDHAMRRSFQLNLWDFVKELDGDESSTTILADADDDQRLWKVLNMGRFFGFLIGEGSLSLNVLRVVNFLTASTDAKIFLEILLITMLDTVSKKSESTDASAKKSKDVAFTGKVMADRVAKCDEQPLLIKGLQYFLNNKIRNSELVKGKKQRLRVEWGVDTMSDMLAEMLKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN66</accession>
    <name>A0A1E3NN66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartyl aminopeptidase</fullName>
            <ecNumber evidence="4">3.4.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_59087</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.</text>
            <dbReference type="EC" id="3.4.11.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M18 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.21" evidence="4"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017996.1">
        <property type="nucleotide sequence ID" value="XM_019163343.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NN66"/>
    <dbReference type="GeneID" id="30180030"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="entry name" value="Indigoidine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002759">
        <property type="entry name" value="PRK02813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF3">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="Indigoidine synthase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="12">
        <location>
            <begin position="1453"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1298"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1300"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1311"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1323"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1337"/>
            <end position="1356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1702" mass="189966" checksum="E38A440985B1CCEE" modified="2017-01-18" version="1">MSASALQQAQGFLDFVNESPTPYHVVSTVKKELKEHGFQELSERANWAGTIKKNSKYFVTRNASSLIAFAVGGEYQPGNGFAMVGGHTDSPVLRIKPISKQTKEGYHQIGVEPYGGGIWHTWFDRDLSVAGRVFVNYPDTGKCVAKLVKINRPLLRIPTLAIHLTKDRYVKFEFNKETQFKPIAGLVNSSSLSDSPDSSKPAAAKDGCCKGEDLTEEEFHSVKSTIERHNKELLDLISQELDIEPSQIEDFELVLYDTQPSCLGGMKNEFIFSPRLDNQVTCYSATEALVKSVADGKLAKSSGIQLISLFDHEEIGSLSAQGADSSFLPNVLERLTRLTANPQVDLTSELPSSYFLTTMAKSFMISSDMAHGVNPNYTENYEAYNRPHLNDGPVIKINANQRYVTNSAGIVLLKKIGNLSKVPLQLFVIRNDSPCGSTIGPMISAKLGVRTLDLGNPQLSMHSIRETCGAADIEKLVALFESYFENYSSLEDTISASTILAMPNVLEAEGEDQRSVVHWDELQKVLEMQCHIDQYDVEQMNEDEVPNFLDTKKKLINIEVPQVLLYQKDPSKREKFLAKVDELIRRICMKFPSPKVSVVLASTTPTEVDYNSIANREEVVEYYQIPDDPKVLSVSLREQVKTSKRFIFPDITVFDKTRYHEFERNEIGDTHRLSDLKDKEWAKDGGKNPVEVEDDTWLERKEKTVEKGESLYKFGEDDDFHSALEDKQFVVDNAIWIACAMAKEVERLIRQNGAIPASIGFVKGVPTVGLNESEIELLGLPDIYNVKITRRDFANVLTRKLTGGTTIAGTMILANIAGVDVFATGGLGGVSRPWTLADVSADLDELSKTPVAVVCSGPKSILDIKRTMEYLETKGVPVATYIDEDMKNSLELAIRTRLDDLKALSTPESHNEINRIWESQGINVPGFYVRDSGNGYVFCAPAPVEAAMDREAIDGVIEEALRLAEEKNVTGKHLTPFMLQTLYDKTNGASAKCNVTFVKNNTVLGSKIASDLCLVKGGKRSLVQPQFETVEPAKHEVRATAKTTTTTPLNPSAVVIGSVALDTMCQFPAEKIKFGDSNPGSIGKGSIGGVGFNVALAASCYDPSHPAVLISALNKNDNAGDFILKQFDRFSMPQQGIIHLPGEATAQYISMHDSKGSLALACADMDIIEKIPSAQLLTIYTSFAESGKFEDIDNWFNVLDSLGVGGDFRERLSHASHRFPLIATYLHQGVFQQAFQLLPFFPNILVKDGANGFSNMTSVQLPPGRVETLTDDQEHVLKQVWMYMLHFQGYNIKPPGPLLRSNSINSTRSAKTHEAPAPVEKKEKKKGGFLGRLKRNKGTDEISQPHHSRHSQEALKRTLSNQSAIDPNVKHSTDLGVHSALRSLNPKETEEGFWNALRHDSPDNFMLRFIRARKWDVDNSLLMLAHSIEWRIDESRVDQVLRASELGCLEKKQEGVLTQFRSGKCIIRGRDKKGRPIVIVRPRFHHSSDQTFEEVEVFTLLLIEYARLMLTEPIDQCALIFDLKGFTMANMDYEPVKYMIKAFEAHYPESLGILLIHRAPWIFNGIWQIVKNWLDPVVASKINFTKHASDLEKFIDNKQILKEMGGSDDYEYKYLEPTKEANGLATNDKAMVTSSETQRESLRKEFVNKTIEWIEASDVATSKKLLQERIAISKQLSENYRTFDGCVRNRGVYDRLGDITFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN71</accession>
    <name>A0A1E3NN71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_25402</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018665.1">
        <property type="nucleotide sequence ID" value="XM_019162391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN71"/>
    <dbReference type="STRING" id="763406.A0A1E3NN71"/>
    <dbReference type="GeneID" id="30179078"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF368">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="9">
        <location>
            <begin position="785"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="8">
        <location>
            <begin position="884"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1147" mass="131368" checksum="F91C3969B1813329" modified="2017-01-18" version="1" fragment="single">PLASVIVTKNYYQTESIPNTLQLMEDDVVHVLSKPNEHWWDGVLVDPVGNVTRGWFPASYTRPPKQHQSTPHQAPVSTARSEPPPSNASSPSIFKEKNYFVPKTDSFLTSSLANQHQHESRQSSKAHTETPPYSLMNSMHMVTNEEISSYFQTSNSESQPSFNFVPIWLPQFTIDNQVVYKDQALKVLTRDIPFVDADYLNEDSIHEVPDTSNLVADLNPLPISLQRQNTEPSINSTPPANNFKSYSIYDSFHGSTNKHAPQQTQVLINMPDFANVRLTELFYTDNTDILTWDALASSFINSLDRSISSLEVYDKQKFKDSMNACSNCISSLHTAGRLIHQQLLDSNKQIQFTAILNKITNMFIQFKIWTTLTLVSLDSMETSKNSKSLGISNPEDVPKSKLSSYHDDSVRCRRKLEKLVTHLCLVTASLSNPNQNGIFAKNDSKILPMIYPRFVRDKFEGGNFKNKFVDARFSQSDFFSNPAQNQVNILLDDEALNKLASTEKKILESLKEVQDILSFKLSKDATLKTFMDDRNLKLLAAIYQCIPLLCLFIDTIESIDMTVFAMINKLAARNSKPERQNTIDSESSSQSFYDATAKVFRPMINEFMYLKQLLHSAFTDLVLDAQTITATDPETFFPIEREGPSSDKPISKSKQLTEALMKQLELINSEIYHDGVFKLEPNLKLLETIRLAKGRMRLISVSVNQLKEERRSILNYCSRVMNTDFNIASLFIAERHNTLVSMNSQSTQTIFTGNRATNDLSEWPGTENIPWYMGPDHDEEDLIYETSNLRGGPVRSLVAKLVNPTSPNDELFEQTFFSFFSTFVKPVKLFEILIEKYHIGMPDALSYEEYEYIDYFHLGCMEPKQIPVSPMTPSIKQLRLQNLNINYVADQITAVQAFYYRKLNLWDLLGRTYNFSKILHKKNDRISYNMKDPLGTKNISNFVKNCNNLTHFTTYMILRKDSEERINAIKFFISLAEKLLSLKNFSSMTAIISGLGSTSISRLRKTWDAVPSFYVAKFQKMDNLMSIGKNYSEYRNILRFMETDDDPYLPFLGMYLSDLRFTTDGNSDWLGSKRGSKGLVNFSKRMSIMKIMKEVLNFNETLYKIELDTDFSCYLNEMFTGLPDDERMYELSIQIEPRVSLLKNPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN90</accession>
    <name>A0A1E3NN90_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_116115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018689.1">
        <property type="nucleotide sequence ID" value="XM_019159814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN90"/>
    <dbReference type="GeneID" id="30176501"/>
    <dbReference type="OrthoDB" id="10636293at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="230" mass="26596" checksum="0FC8D7660B4F3037" modified="2017-01-18" version="1">MDQHDLDLADSKIDKCFSVVEFNLNNHTPQFASNFAMEYYIRKIQRCGQYRVAHLTSFLYDTDRIRHIVDHMPLVSPTLHDRLSENVLVWQCLSLAQVHDIVNELLLSFSGSSASEGSFNLVVIEDLDEAFNIGSFENYILDDSQYHANYSQALPNIRRDVYVSPAQGENELQRQARRPHGPQYARHPGPHQWAQSSRRLCLRQRVRSVALQRCSIAAQVDKRVYTMYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNA4</accession>
    <name>A0A1E3NNA4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018700.1">
        <property type="nucleotide sequence ID" value="XM_019163970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNA4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNA4"/>
    <dbReference type="GeneID" id="30180657"/>
    <dbReference type="OrthoDB" id="303107at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="509" mass="59006" checksum="3707A7383A338D32" modified="2017-01-18" version="1">MPVLKSPKYTDHPAEHLSKLVPEETKAHLLKTPKQLLIENPIYAERLASLRNHYNYSYLVQWIYLLKHVIKLSDAFDVENLEEELLGIAPPVFLNSLKTKLIQFLKNEKLQNIDMEFDFFVDQIYQDYNLIELNEEQIKNEENGDVDDTPLITYSKLPLLEQIDVLYNLAKLSNTKSYANFRKVVDKFEKPQLSLKIHPIYTIDKQNTRDEYIILQDARLYLRRWEYTDLSLPAKRSEYDSKVKNPYEDLQPLEPAMIHWECLTAGIYQYDDMIKELKKSFGKKTSSDEYALSKQLEENIDFIVQHDLKKRKQGAQRKREIEMQMLMANRKRSSRLEEKEKRKKEEEEERMKEFEIIKQQAAEMRAAKRQKLKESMYATSNDSNHMSREERLKKRQEITSSSAIPAEKTTQNADDSIIEVKNPPILDVQETTTSVASSQAQVPPQTTTAEILPVAREEQRPIIPEKAINPASESASPPAAPKTILVTETSTVAGNTENTALPNVFDPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NND6</accession>
    <name>A0A1E3NND6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_10588</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018725.1">
        <property type="nucleotide sequence ID" value="XM_019159695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NND6"/>
    <dbReference type="STRING" id="763406.A0A1E3NND6"/>
    <dbReference type="GeneID" id="30176382"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="640"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="745" mass="81671" checksum="1F37EAF4B0491E50" modified="2017-01-18" version="1">MSYAIPQKNHSHQLHPLDRLDPAELDNATTLPDVSTISIGPHIEKDAEGTCMTGDTRDPKTEQSIGTDENERDSNGGDDSESGKENNGTAPVGENSKPSSRQDQKLLAAPPNADQGSCGNNNFNNNNRGNRNNRNGNNTRYQKNPYHNRSYSSNSYQTFQPSYIPQYPRMIPINQYPVNGGMIPQYPASATYAGSYTSSSGSSPPAITPSVSRRQSQAQLESYANYNINYSYNQDATRYSPPPYYMYTPYVPMYQPPYPASASHSPQMISVSPQAQGFQRQQYGSNRVMHPMQMYYQSNSSNGNQFRGGDYDESAIEDEELKEEEESVNAVATTKGGASGAVTMNLLNLPASETTPESISAIVGQILKGETSNDKNSDNNSNDGDGEDDKKEEEKAFALPIDNFKIYKYPDQELCWCSFQLTDEDKLDLLITNLNGHVFHGSILKCFVQPPRSDDADGSKRYTYSSPQYMYPSPAPRISGYQVNYHQHQASMPPMGSPMVPSVPYSGYSPNSMSSAPPVVQMGAGYARYGAGFNSSGGGGNSGYRHGYRHNSVNSASTYGGSVGGAGAGAGAGFGRKYSSSQRSKPLGNNYNSIVFPDLDEQASSTSTSPGYYEEDQIDDNELSTEELQEFNETRHVSPDRVFIGNIPFNSSYHSLWLFLTHGGEQVLLKELQLKTDSSGLSRGFAVGITDGVASSRELIGRFNGRDFENRKLIVRFDKHNGLIFKSHMKKKLDNMGFNPAPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNH5</accession>
    <name>A0A1E3NNH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71698</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018763.1">
        <property type="nucleotide sequence ID" value="XM_019163986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNH5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNH5"/>
    <dbReference type="GeneID" id="30180673"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="509" mass="55976" checksum="0D5626E308349A07" modified="2017-01-18" version="1">MSGTNAHSNYWKINQQEDEDAGSGAGSASAFQLASNTGFSRDHETVAVAANVLTENLKIFQLAENSLVHLASITLPDVQSLRFLRPISPPTQDFKFLATGHSNGIAHLSAIPLTDNSVFENAEIIKRFNHRKQLSAEPAGATKTGCLNDGTLSTTISSMQLTGPAWRSTSLNSLVSVYDHHLFVWDTSRSRAPLSLVRTNGISRVALNDHIDSLAAIAGDFGLSLLDLRTGKNQSKSSLYLPPNSSVSSPSRYGAPKGVTCVEWCETNENFIATVQSDAVYIWDIRKLEPLTQLTGFSDSVTHVKWNGDNLWTGDKDGYLTNWNLENIHCLEKKRCVASQQDTLADLWENFNLEEKPSQNTVFRGTSTKVSNSKIVSLNLDVSKNNVVCLDGSFLSTHDIKLLNKAPQPVEAVKRRVSSTRKLKRPAPIYVGPERIVSTESLSSVGSNSSVPLFDTSLDSRKTSNNISFPPTPTSDTKKPTDYLEYFQKEINTMLESMDTKKVNDIVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNJ6</accession>
    <name>A0A1E3NNJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_31037</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018784.1">
        <property type="nucleotide sequence ID" value="XM_019162671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNJ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNJ6"/>
    <dbReference type="GeneID" id="30179358"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098562">
        <property type="term" value="C:cytoplasmic side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036267">
        <property type="term" value="P:invasive filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045900">
        <property type="term" value="P:negative regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010603">
        <property type="term" value="P:regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000749">
        <property type="term" value="P:response to pheromone triggering conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="61"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="248"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ47671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="444" mass="50773" checksum="7A773A1A026FB834" modified="2017-01-18" version="1">MSTENWKESLNIPTKDLRPQTDDIVGSKDETFESFNLKRELLMGLFEAGFEKPSPIQEEAIPVALMGRDILARAKNGTGKTAAFIIPTLEKIKPKVDKIQALILVPTRELALQTAHVVKTFSRHMGLQVMVTTGGTSLKDDIVRLYDPVHILVGSPGRVLDLASRNIAKLDSCQMFVMDEADKMLSPEFQNIVEQILEFLPQSQPGRPNNYQSLLFSATFPRTVKTFMDRHLYKPYEINLMDELTLKGITQYYAFVDEKQKLHCLNTLFSKLQINQAIIFCNSANRVELLAKKITDLDYSCYYSHSKMKQADRNKVFHEFRQGRVRNLVCTDLLTRGIDIQAVNVVINFDFPKTAETYLHRIGRSGRFGHYGLAINLINWNDRFNLYQIEQELGTEIKPIPPKIDESLYVVNEPSAVPRPFKADDRNNNNNQEAFQQQQQIQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNW4</accession>
    <name>A0A1E3NNW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="5">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_15210</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018336.1">
        <property type="nucleotide sequence ID" value="XM_019160716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNW4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNW4"/>
    <dbReference type="GeneID" id="30177403"/>
    <dbReference type="OrthoDB" id="19639at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF0">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="384" mass="43681" checksum="35AE2246BDB6466D" modified="2017-01-18" version="1">MPDVNIGTKVEKVTDMGKESESESSATGVSNEQVLEQTQARIRKSKELNKGLKRTEDKQSLPVYTKDSFLQSFNNMVRALISPQDEEVRKRWFKNDNESTLELAFFYFYRSILTIFYLIYSIYALFEYAINRGKLRFLSIAYRSNDDPSVINADINKLPKIPQKLSVILNYKSEQEEGGGIDGLCNDGASIAAWCVSSGIPSISIYEVNGVLKKSVSELSRAIFEKFESYFGTENIPNFLIKIPHLNLSYSGIDGVLIDNSVKGTGRDKDDFAEYDIEISLISKVDGRSTIVELTKVMAQLAKNGELKKSDITIKFLDHELRQLIGEEPDLIILFQPYLDLQGYPPWHIRLSEMYWEPDNDSVSYVIFLRALQKYSTCKVNVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP60</accession>
    <name>A0A1E3NP60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_30782</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018988.1">
        <property type="nucleotide sequence ID" value="XM_019162655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP60"/>
    <dbReference type="STRING" id="763406.A0A1E3NP60"/>
    <dbReference type="GeneID" id="30179342"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016768">
        <property type="term" value="F:spermine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="15"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="305" mass="34526" checksum="0F7C994858E75944" modified="2017-01-18" version="1">MSDSSKELTHPLVVDGWFREVSEEYFPGQAFMLKVEKILHAEKSQFQDILVFQSTDFGNVLVLDGIIQVTERDEFAYQEMITHVPLFGHPNPKKVLVIGGGDGGVLREVVKHECVESATLVEIDDMVIKLSKKYLPNMAKSYTHEKVQVFLQDGFKFLKDCESRLDQDKFDVIITDSSDPDGPAEAFFQQAYFKLLFNALNKDGIVISMASENIWLNINNLKKLKDVCHTVFPVVKTCYCTMPTYTSGQISLMCCGKNADIDVAKPSRKLPAEKERELFKYYNSEIHSASFVLPTWAAQVLDNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPT2</accession>
    <name>A0A1E3NPT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_31299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019178.1">
        <property type="nucleotide sequence ID" value="XM_019162691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPT2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPT2"/>
    <dbReference type="GeneID" id="30179378"/>
    <dbReference type="OrthoDB" id="1918363at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044612">
        <property type="term" value="C:nuclear pore linkers"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="4" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="942" mass="108301" checksum="BAFFF3625C920F46" modified="2017-01-18" version="1">MAGVLTTSKSGTLDPFGKDKSKKSSSLDDLVQSSRNLPRRLDDIDTLYLSLNEIQKRAYKLRKYNKADTKSNPYTKAHYLLIGKGLTIEEIDNELNNIKKLAHKHEYNSQLSRNNNTQNASLGAYARQKPSNLEFYMSRQKDEDVLLAIESSLSSAAKDFDSYVNNNIHLDWKRRKGELCEKLSAVLKRDASTSLTSPDKKSKKLTQKELIEKQLIWGTHTSKSIISSNFKFNDVQSAKHLDSLMNPNISYAMRKKFELCAEVIYELNEARQQKTWYKLATVFSSLNKSDLASSKSEQLHDASNTFESIKLKENIEQKSREYLESQFRDYINDLYSVNKTPSMKDLSKFDQAKSLTPNVDKVVNFIEMSLKTKQKSWKVPNMTFVNGLPLWAILFYLLRAGCYEEAVIFVSRYESSFQKLEKSFSLYLKSYCESKERKLPQELQGRLNNEFNQYFKNSTKDIDPYRYATYKIIGRCDLARKTLPSITLSIEDWLWLHLSLIKEDDSSTFESDIIFNDANENVTNDTYSLLDLQKSVLEFGVDAFNSSSKNPMYLQTLLLVGLYEDAIKYLMFSNEIDAVHLAITLNYYGLIRISDEKTNPSFAENLLNVDSQGFKSINFARMVGNYVKTFKFSDPRVAAEYLFLICLSEGSDETFKQEQIELCHEAVRELVLETREFVLLLGKITKNGMRIPGVIEERKSLIYLEDENAFLDNIAEKAAQKASEDGRLIDCILLYQLSEEYDTVLKIVNKLLGDFINSIDLANNFESISDIPGTTETDDENNIIKLSDNLLKVYNASPEILSKTSAKNRVTCRKLLDIFEIIKEFGNDKELGNKCMKIIDQIKCLELIPIMTNNDISKIRNYINEFSNYDECIMKNIPKLLIIEMTCLQKLISSLTYSSMGNDAKLYDLKQISKNCMIYAGLIKYKMPREVYTLLINLEINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPY4</accession>
    <name>A0A1E3NPY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED10</name>
        <name evidence="7" type="ORF">PICMEDRAFT_72082</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019209.1">
        <property type="nucleotide sequence ID" value="XM_019164092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPY4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPY4"/>
    <dbReference type="GeneID" id="30180779"/>
    <dbReference type="OrthoDB" id="337270at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019145">
        <property type="entry name" value="Mediator_Med10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09748">
        <property type="entry name" value="Med10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="152" mass="17039" checksum="8D272F6BB9A864A2" modified="2017-01-18" version="1">MATEKAYEDTTTPLGSLQEEISSIIETFVHLGVQVHDFQGTQEAKLGLANHINKTISKLHDVSSRDDLRDIMIPTDLLNYIQDGRNPDIYTREFVEVVRKINQYLNGKSLAFENFRDTLAASIKQEFPELAPEVDSIKAKTSVSASLEDAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQD8</accession>
    <name>A0A1E3NQD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_71785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018866.1">
        <property type="nucleotide sequence ID" value="XM_019164005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQD8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQD8"/>
    <dbReference type="GeneID" id="30180692"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="399" mass="44480" checksum="295646AD8E339130" modified="2017-01-18" version="1">MSSLANSNLFKPIQVGDVVLKTRLAYAPSTRLRASKDGVATDSMLEYYSERAKNNGGLIVFEGTSPRRDFGCFPNQPILETKQQVEAQKKIVDAIHSNGSYAASQLLHFGRAVSPQLAKLFNIPFVGPSAIYIDEANKEAAKVEGVELKELSKDEIKDIVKEFADSAKRAVNEAGFDFVEVHGAHMFLIDQFIQTSANQRTDEYGGSIENRSRFLLEVIDACIEAVGAKHVAVRLSPYAEIQGGLGKNSEVNPIVIWGYILSEIQNRADKGDELAYISVVEPRIQGDNEGDEKDSKINFSWPRLLWKGILFRAGAFLDPDHLEKLAKNIDGDDKLIIGISRYYTSNPDLSDRLKRGLPLTHYDRSKFYNAFSNDGYLNFQPYGKEEVHTKDDVEPKPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQH3</accession>
    <name>A0A1E3NQH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Na(+)/H(+) antiporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_47364</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019467.1">
        <property type="nucleotide sequence ID" value="XM_019163127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQH3"/>
    <dbReference type="GeneID" id="30179814"/>
    <dbReference type="OrthoDB" id="5327978at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000015">
        <property type="entry name" value="Na(+)/H(+) antiporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013928">
        <property type="entry name" value="Cation/H_antiporter_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08619">
        <property type="entry name" value="Nha1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="26"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="14">
        <location>
            <begin position="466"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="505"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="681"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="767"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="575"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="681"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="771"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="805"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="898" mass="100190" checksum="325E7E4693CE1F1C" modified="2017-01-18" version="1">MVWSQLDVTKAHVAYAVVAVFSTCFSLCSLFVKEKLYLGEASVATIYGLIVGPHCLNWFNPLSWGNYFYITLEISRVLLCIEIVAVSVELPKKYVLKHWFPLFLLLIPCMTAGWLIIGVFIYLIIPGLHFSHGLLISACVTATDPILAQAVVGKGKFAKRVPAHLRNLLSAESACNDGVSVPFVYLALNIILHGGHSSEVAKEWICVTVLYECGFGCIMGSVIGYTGRKALKFAENHDLIDHESFLAFYIMLALLCAGFGSILGVDDLLASFCAGTAFAWDGWFTHRTEESNVSTVIDILLNMAYFVYFGSIIPWQEFNDTALGLNCWRLICIALVVLCLRRIPAVLSTKMINPDIKNWKEAFFVGHFGPIGVGAVFAAIIAISDLEADVLHISHGPSTNYPVESEYYQLIRIVWPTVCFLIVTSIVVHGSSVAVLTLGKQLQSMTFTMTWTKADSSGAENGNVNWFNRLPKIERSGTSFSLKRVDTMAHQDYNGGSITLENIPTIETSGTPIRPAGGANRKKKRRLLKRKQTKKRKIPPVSETLDLRNSNRHPAQEEEKPQIEVDGPEDESINSDERHSGSDQDKLNVPLQKIVTNASQNSMGSRLSSNSIMSGQMRGTVHASTAALKKIASDYELKEDDLQPVYDEDGDLRVPTHAYNYRDGLVIEDQHGEVLRTVRKPVPLEEDKEKSSPERVVKRSTTGASKSAFDKFVDLGDGILFRNSVPRKTRKLHGYRINDDIIIENEDGEVLGRYKVNVKKQKAEELAKGINDQQYNNNLSEKNRSERPRSPGRKLPSALIHDSDSDYDTDESSEGDDSDDYDDDEDEEDNDDDNDDDDDITLDSRGHAHVRKVSSKNENPIRNRGRSSIYDESKFEKARRKAALSKSNRESDDEEEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQK9</accession>
    <name>A0A1E3NQK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_29253</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019468.1">
        <property type="nucleotide sequence ID" value="XM_019162580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQK9"/>
    <dbReference type="STRING" id="763406.A0A1E3NQK9"/>
    <dbReference type="GeneID" id="30179267"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000007">
        <property type="term" value="F:low-affinity zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF69">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="373" mass="41228" checksum="315FC98AB3B698FF" modified="2017-01-18" version="1">MSALTTLLSPGNFVELFIREEDTCTADNSYNGDHFGARISAIFVVLIASAFGSFFPVLSSKHSFLRLPPACFFVAKFFGSGVIIATAFIHLLQPANENLSNDCLGYPFDIYPMAFGITLIVLMLMFFLELIAYRWIEAKLSNQSVDSNEPHTHSHFGETSMYVHDEGHDTEHSHTHGHSNAEYNIEDMFSEISEDIEKQSTGSSELDSAYAAQILNVFVLEFGIVFHSVFVGLTLSCSGDEFISLYIVVVFHQMFEGLGLGTRVACVEWPASKRWTPWLLCLGYTFCTPIAIAIGIGVRNSYPPGSRRALITNGVFDSVSAGILIYTGCIELMAHEFLFSDEFKGPGGFKKMIWAYIVMCVGAGLMALLGRWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQM7</accession>
    <name>A0A1E3NQM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_32255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019121.1">
        <property type="nucleotide sequence ID" value="XM_019162752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQM7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQM7"/>
    <dbReference type="GeneID" id="30179439"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000301">
        <property type="term" value="P:retrograde transport, vesicle recycling within Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000823">
        <property type="entry name" value="Small GTPase RAB, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23224" checksum="1BAED427D4814654" modified="2017-01-18" version="1">MLTKHKIVFLGDQGVGKTSLITRFMYNTFDTHYAATIGIDFLSKTMYLDDQTIRLQLWDTAGQERFRSLIPSYIRDSMVAMIVYDITSKGSFDDIPRWCDYVRTERGDDVIMVLVGNKSDLPGRVVSTEEGEKLAKELNFNLFMETSSKNGYGVKNLFKKVAKLLPTVQGDEGTLDGAAAAAGTKDSNTQTIDISTTNTKDDGPAEGCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQN9</accession>
    <name>A0A1E3NQN9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019524.1">
        <property type="nucleotide sequence ID" value="XM_019163535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NQN9"/>
    <dbReference type="GeneID" id="30180222"/>
    <dbReference type="OrthoDB" id="4096087at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012985">
        <property type="entry name" value="Peptidase_S64_Ssy5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08192">
        <property type="entry name" value="Peptidase_S64"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="769"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="856"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="784"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="864"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="883" mass="96650" checksum="D8FA939AB2035B4A" modified="2017-01-18" version="1">MDIKKIFNKKKVKTKQGGHTEGGGNSEATSVNSVTSTASSSSNSSAKIPTNPAPSHNADVFTSSKSFQYSYNSNTNSLFSSKRTLSTGVSTQPSSNVSYNKNNEQAKITSIGRPLRVLHEDEQEVEYARSGSVYNIEGKSTAADDDSTASGLDANSNRAASIHRNSTSRRRSNLSDDVSLCPTEKSNISTQGTFANIDNGSNTNLNRSQSSSISESELRVQLVEFIKSQLRNLANSVSNIIIQVSQSVLNLTKASITINESIILTHKMIKSNKYISLLASNQFDTINSVGLRRLIKNILHLLDNLLIGDVYNKSKSSVIKRLHDLFLLIKIIPTKAPELTNFISYMAPTMFPIDSNVLNFPNVEKVDQIMSSLLSKGKENLFSDQDGSFIAPVLRGFFLPSLTIITFIFGFPEISKEHHDIIKFFSTQSSDIHFQLQKNRITYASSKSMKLKSPFRTVDEGQVYVPISMSLSTDSSTISSGTLGGYLYPKVPENCSNPKLLKYRGQIFGLTCAHVVLGSESESEELHPNVSTPSPVLINLYKNALLNEMMNHSTNSPEYRAYSEAVKMIDAEYPLRKVSLNGKKVNRNLPTEDLGCIIWGERLINDNKLSDMAIIKINDRLKKKFVNYLGEDLQLSQYDPSLILSNLNVKKTISFQPRKNGILNTANLSVFKVGSTTGYTNGNLNGMKMIYWSDGSLRSSEFIVAGGDGKMKGFANGGDSGAWVLSKLSDVNHIKSYSSDSEDEPLIEEDENKSSLSAFIESFIPAVSSGRRQDRASGSRRRSTPTASAAATTADAGLGPFNGPKEKNEESGLGVLGMLHSYDGEYRQFGLFTPIDDIMERLEAVTGVEWGVVGCSEEGDLHEGSSSIGSTSDGSDKEESEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQP6</accession>
    <name>A0A1E3NQP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_13998</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019534.1">
        <property type="nucleotide sequence ID" value="XM_019160178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQP6"/>
    <dbReference type="GeneID" id="30176865"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="319"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="145"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="263"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="791"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="847"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="899"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="855"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1007" mass="112110" checksum="25B2A059A779BF50" modified="2017-01-18" version="1">MKGFFQDKSCLLSPDQRINVPSTPFAIPDLTPKCAVASKVAMTPTIETSGCDSHDHFSHTDGSTIATSFPDSAHMNKLTPNYSTSYPPTSESEFEKLRKIDLSYLTLFRRSARFSNKGYNHFFLSQELKCDDHFLSPSAHDQNNLNGGAYSTDSAANSHGSVPPNYRRNTSSTTLNSNGVQSNGNMSTKSVDSHAIYNMAFSHDGKYLASAGADGLIRIWEVITSEMDRHSNNFHSLNIPVKPHRKSFIENALDDTLNDLGSIINGGSEQRTSKSYSKSRRNTLDSYDSANTNESKPNSHFSSKNAEYAPVFKSKPVKTFYHDRTVNSLDWSKNNFLLSSSEDGTAKLWHVDRADCLQTYKFDSIVTCAKFHKTDDRFFVASQWNGRVLFLSIFEKEVIYEVNLNKSITCFDFCPNSKRLFVGCDKGYIFTLKIDGGLIVESDYQIKRKHNMPRITGIVTFIDDTKDCLSDPAKTPYNKSNTSSNNKTNNESELPMVKVLISCNDSKIRLFNFTEKFLEVRYSGYTNKASTISASLNEDKTHVISGSDDGWTYIWQLYSDKRNKIENEKKLFTKAHFDLLNYFKDDTCLIDNKYYGSFHTHNNRCNVSLFAPRASLKLLELSNDPIFDLKHQYSSLVTESHIKDLEIDDLSTAVIVSTDNTGKIKVFRRDFSRYIRKALQGKKASQLLDRKQSVLSRGMSKKVLNRNSLVIPNYEATTMNIADTMSSIYMDADATVRGRGKKGYLRPANKFAVESSIIPDGSNANGSGTTKASSCCKSAKQLIPKINIEKQQQQKVDGNSNTNMITSTPDVSTSSYSGKTSYDEMNNSTLRGIQHNHSVGVGKDKLTSLPLRGGSSSVSSTSYSSSSKNSIQVSDSVKNIDDEIKKVLVQTPHVGVVHQVGQKQHSHSNTSLSKSEEGGQLKGNVYIERATHQVEDGGVKESQVVSIPLDVGSIANRRAEGWSDVGDGKYTCSKCQSVNFTPHPMTSGTTNQIKFYCDDCGQEAKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQZ6</accession>
    <name>A0A1E3NQZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_70127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019609.1">
        <property type="nucleotide sequence ID" value="XM_019163565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQZ6"/>
    <dbReference type="GeneID" id="30180252"/>
    <dbReference type="OrthoDB" id="4021219at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="568" mass="63284" checksum="D9529CEE2C74CB73" modified="2017-01-18" version="1">MVSNARSSKNKAKSSSTVSTYADPLMTLLHSSDLNRDKQLSETLYEYIDDITDMELKKWCIDSLTVLRNLEKIETQLDQWEFHTLDFTLEVNSQLHKTSSSSSYSTSSAAGSEKSDSIRRKLARRVLDKSVKLRDLLSQQKEGIDSSIFRARHLSSTSPAKVITDAGTILIELTMRIVKLGKHLDEQVTVGYSRAKLTIIGSELKKLVTSDPYIIDKDVVQNYTTFVNNLLNQLNMAVAHNDTVVLWESVAIIGDVEKMFESMKQNTSMAASKEKKPQGGETRSDRRQEQEAEHKGDGDGDGDGYGSRSPSLHDFSPEMVEIDSSPIVENSGLSNDSGLNTDTTLVNSEWGIYGDAKKEVTVAHISAKEIFHDDNDLIRTKITEHIPELIQAFNRQAPARAKPEARKETSASKADTTSTDTKEDWKPQESQHGPAEPEEQEQEQPKEEGSYFSSYFKPSILNAFYQPQLKEPIYVNKENLPGGRAKETVVENGNLLTNATVAVRLDQMSFDDRSKNELLERERGIRRQEAVLDSQLSAPRPTSSSLLNQLSSSVLAKSTWPAHPPGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQZ8</accession>
    <name>A0A1E3NQZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgi SNAP receptor complex member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_56784</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of several multiprotein Golgi SNARE complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the GOSR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019650.1">
        <property type="nucleotide sequence ID" value="XM_019163199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQZ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQZ8"/>
    <dbReference type="GeneID" id="30179886"/>
    <dbReference type="OrthoDB" id="422156at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF2">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027109">
        <property type="entry name" value="Golgi_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="53"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR027109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="26070" checksum="1E5F42239F36F5C4" modified="2017-01-18" version="1">MSTSAYTQSRAHVLSLSTQLSNVLARYSSFATDVHTTPSAEEVETKNKIQTLLSDLSAAINDMSRILDTLQTESLSSSKFQQLSRHKDELKRHRQDYQRLANQIEQERNRLNLLSNVRSDIDQYNDNANGNNPTGGNINSDDYMLDERLRIDRSHGIVDNLINQVMETRDEMMRQGSTLSNVSNRLERTLSTVPGLNTLINKIDARHRKEAIILVVVILICLIILWFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR12</accession>
    <name>A0A1E3NR12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019622.1">
        <property type="nucleotide sequence ID" value="XM_019160209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR12"/>
    <dbReference type="STRING" id="763406.A0A1E3NR12"/>
    <dbReference type="GeneID" id="30176896"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="338"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="352"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="537"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="663"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="214"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="668"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="708"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="733" mass="78532" checksum="A360A4687BE34A4A" modified="2017-01-18" version="1">MSSPQTQNTPTTGSGMPSSLQEAGISRADNGNYILPPILVQYRGGSTQMLIIEVGQPIPPAGSITGASLMTVAQMLRSNTNMNPSNNADNNNSISNSANRGNNGRSADAGGSRIASGNTQPGPVSRLPTPTPAADTTSLANLPRGIRGLDYPAFDVSSEGVYNEDSQAVSRFLAPLQRVFERVYGQNENSNSGTLGTTAEGRNFASDMPPSSRPATTTGPATATAPDPPDGNAADEAAANEQQQNPPAESRNIILTINYVYGGSSDTNENNSGSLLLYVPSIDETNEDNVSVLVRLATEIALRTIAVSLRNSAGVSKQTFEALEVKKVSELKENELECPICYEKYVDKETDEKDLSSKKRKQGEIDEDEDEENPDTKYKSKKRAKDNRANPIPINNSQDQTEKEMKSSEKVKQPVYTHYPIVMKCGHVFGASCLSEWFKTNSSCPLCRKKIPNVMETATEDSRDVTITLPNLARIISNCRPLIENFNNRLMTFSLSEEDDYSGANIVTDNEDQIPSPGSFLSSNGANILERLTTMRRNQQLNGRPNTSDSANYSNENNANANANADANNSGQDRSRVPSTTNRQSLVNFIRDIVSSLSRRTGEEPTASINNSRLGLHSASGRIPIVRPLSRSGTQFSGMFPPLGVESRRTANGVETREISMFGEHTTRTPAPATATAPAPASSAAESAVTNNSNLQEPAPETSEVVDGSTTRSSGLAETNAGSITESSNDAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR81</accession>
    <name>A0A1E3NR81_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannan polymerase II complex ANP1 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14128</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019692.1">
        <property type="nucleotide sequence ID" value="XM_019160233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR81"/>
    <dbReference type="STRING" id="763406.A0A1E3NR81"/>
    <dbReference type="GeneID" id="30176920"/>
    <dbReference type="OrthoDB" id="2405412at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF2">
        <property type="entry name" value="MANNAN POLYMERASE II COMPLEX ANP1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="521"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="528"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="551"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="78087" checksum="34C2D3E191CDA7F5" modified="2017-01-18" version="1">MWNPLKTRKPEFRLDSKDEKRGRFRMVQGVKPTTAVTIVIAIVTLYLYVLPGLDLRGAEEGRYTPDFGELHEHNVEYYDLKDYSPHADGWNHNERVLFLVPLRDASAHLPMFMENLKNLTYPHALIDLAFLVSDTKDSTIKDLKRSLLPIQTGEDEEMRFGRINIYEKDFGQIIGQSFSDRHGFAAQGPRRKLMAIARNWLLTTALRPDHAWVYWRDADVETVPATIIEDLMHHDTDVIVPNIWRPLPDWLGNEQPYDLNSWQESEAGLDLADHLDEDAVIVEGYSEYKTWRVHLGYVRDPFGDPEVEMDLDGIGGVSILSKGTVFKSGAMFPAFSYLKHAETEGFGKLCKTMGYRVVGLPHYVIWHIYEPSVQDLQHMKWMAEEKKNRVQKAKNKRMYNKKWTEAFVEVDKDWEVLKFNVFKNTDLRRYIHLDWNDVDGYLKLYEEPENNIFPELDTIEADYSLTEMSLRYKNGYIPPNEIFDSFPINNAASRDKFKFVRFDEPERESENLDDVFNVDNKVNSNKNEAQRKEQEREEKEQIQQETLNELEESRREVEAEVQKVEDEHNEEMQKVKGKEMTPEQLEEELERLQDMYEQKKKEREERKLIEEAERLSNLAEKKKRKKLLKQKAMQAEKELRRKMAIDREMERRQQMFELEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRG7</accession>
    <name>A0A1E3NRG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5-oxoprolinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_58538</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019391.1">
        <property type="nucleotide sequence ID" value="XM_019163305.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRG7"/>
    <dbReference type="GeneID" id="30179992"/>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="3">
        <location>
            <begin position="239"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="6">
        <location>
            <begin position="680"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="4">
        <location>
            <begin position="772"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1297"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1331"/>
            <end position="1340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1340" mass="146502" checksum="65F39754FD9FCCE3" modified="2017-01-18" version="1">MTGITISIDRGGTFTDCIGNSGSGDPKDDFVMKLLSVDPSNYADSNLEGIRRLLEHFTKQSIPRGIPLDTSPIKSLRLGTTVATNALLERKGSRCALVTTKGFKDILVIGNQSRPHIFDLSVSRPEKLYESVIEIDERVTLEDYVENPKKETSTPNGVDLVKGISGEVVKVLKRVDVEEVKTMLQLLYNQGIRSIAICLMHSYTYPDHELIVGGIAESIGFSNVSLSSQLSPMIKYVNRSNSCVADAYLTPEIKEYLHGFESGLKYGLKKIDQTDGDDNKGVSCQFMQSDGGLVDASRFSGLRAILSGPAGGVVGYSRTCYNPNNPIPLIGFDMGGTSTDVSRFGDGKFDHVFETTTAGITIQSPQLDINTIAAGGGSILSFKNGLMQVGPESASSHPGPACYRKGGPLTVTDANLLLRRLIPEYFPKIFGKNEDESLDLEVTKDKFELLTREINAAHDHLDDKSVEEIAYGFLEVACENMARPIRQLTEAKGHIISTHRLVSFGGAGGQCAVQVASVLGIKEILIHRYSSVLSAYGMALADVVEEVQEPCNMEFTIDNIEILNDRLKLLETKATGKLGRQGFKSENIEVEAYLNLRYQGTESAIMVPREAQGFLKTFQAMHLREFGFLFDDKLVVVDDVRIRASGKVKQRSDESVDEQILKLHKANGFKEADPKRLKFVRDIYWKVGDDLKSMPTEIYRLEDLKNGTFLHGPAIIADGTQTNVIPPGSVVTVLKTHLFIQILEKDAKGDPKQNLMSSVEKKVIPDEIKIEPVLLSIFANRFMDIAEQMGTSLQKTSVSVNVKERLDFSCALFDSVGNLVANAPHLPVHLGSMSACIATQAKLWKGKLSPGDILVSNHPSAGGTHLPDITVVTPAFEKGEIIFYVASRAHHADIGGLLPGSMPPNSKELWQEGAAIFSELLVKKGKFQEKRMVEKLLIEPSKYPNCSGTRRLSDNLSDLKAQIAANQKGIQLINLLVDEFGLQTINAYMNAIQDNAASTVTRMIDQLVDNDESKKLSCVDYMDDGSQIALAVYRDPDTKKVVFDFEGTSPQTYNNFNAPTAITYSAIIYCLRCLVDQDIPLNQGCLRPIEVRIPKSSLLSPADGSAVVGGNVCTSQRITDTILKTFDVMADSQGCCNNFTFGSGGNGPDGKYVQGFGYYETIAGGHGASEKWEGVSGVHTNMTNTRITDAEIFERRYPIILREFSIRKGSGGVGKHNGGDGVIRDVEFRIPVTASILSERRVIAPHGMHGGEDGARGMNLWIRHTIDGDGNKEERIQNLGGRSTVDISAGDRIIIKTPGGGGWGHASEKGIETTRSLPNKRKLLKDGGSLQKRKRQQLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRM2</accession>
    <name>A0A1E3NRM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019843.1">
        <property type="nucleotide sequence ID" value="XM_019163616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRM2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRM2"/>
    <dbReference type="GeneID" id="30180303"/>
    <dbReference type="OrthoDB" id="196103at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06178">
        <property type="entry name" value="MFS_unc93-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF59">
        <property type="entry name" value="UNC93-LIKE PROTEIN C922.05C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="57954" checksum="CFBF5E91C8BD6992" modified="2017-01-18" version="1">MSEQEKSTTLQVNVSKGSSNSFDVALEKDAGGAGATTLQEVKHYTPSEIESSGKFYDKRYVPFLPAYSNATIQIVMLAFVVFMTPGMYNALTGIGASISGPDGVKTQDNSTVALYCTFAGLGFFAGTFCNLMGPRYCLMFGGTGYLIYAGSLLSYNHNQNAGFVIFAGAYLGLCGACLWAAQGSIIMSYPTEEKKGRGIMIFWVIFNLGAVIGAIIPLANNMHSDSSTVKDGTYIAFMVLMGCGSVIAFFMLPKEKVWRDDGTRVVDKALPNWKSEILGSFKLLIKEPKILIMFPMFFASNWFYTYQFNEFNAGRFNIRTRSLNSLLYWFFQMFGAVIMGTILDLKWFRRSVRARIGWGFLFCISMAIWGGGYKFQKSFTRADVTADANGTIKIATMDFKDGAYIGPMFLYIFYGIFDAMFQSYCLWLMGALSNDSEKTAYYAGFYKGIQSAGAAVAWRLDAYGTAYMSMLASSWALVQGSMVVAIPLVFFMVSDHTEEEPERILEEDEFSPIVALASGEGRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRZ0</accession>
    <name>A0A1E3NRZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Monopolin complex subunit Csm1/Pcs1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14404</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019988.1">
        <property type="nucleotide sequence ID" value="XM_019160357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRZ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRZ0"/>
    <dbReference type="GeneID" id="30177044"/>
    <dbReference type="OrthoDB" id="2431049at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034506">
        <property type="term" value="C:chromosome, centromeric core domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033551">
        <property type="term" value="C:monopolin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990644">
        <property type="term" value="F:microtubule site clamp"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23787">
        <property type="entry name" value="RWD_CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040349">
        <property type="entry name" value="Csm1/Pcs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020981">
        <property type="entry name" value="Csm1/Pcs1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038608">
        <property type="entry name" value="Csm1/Pcs1_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28006">
        <property type="entry name" value="MONOPOLIN COMPLEX SUBUNIT CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28006:SF1">
        <property type="entry name" value="MONOPOLIN COMPLEX SUBUNIT CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12539">
        <property type="entry name" value="Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Monopolin complex subunit Csm1/Pcs1 C-terminal" evidence="3">
        <location>
            <begin position="294"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="401" mass="45328" checksum="9B3B9FE18D475C0D" modified="2017-01-18" version="1" fragment="single">MESAKRLGKAPASASGTPKKQKMQADSEVEDQDPAPKRLSLFDVIEKEDRIIKKRANKNDTDTDVQEAEADEDEDVDADVGTEKDIMFATSGSSEEGRYISKFVEKDHASDSGMDRTEKTNAGTNSVNTSSLASAWYDEGTELPDGVGDVFHFKHSSQPKLPTTKTISSMFQKKQLKKLTELLNDVKSQYPAGFDEYKQAAKLREQKSQKLISFLLEENKRLESKVVTLKDKVAVTKKEYKDLSRESARELSRWKSIAHGAKEREDELESEILTFKSKLVKHDGSAKEVESHETKLNFLELLTGVHCVDMKDEKSVLNFTIQQTGKVGSLYYRLIIDKSSPQDESQDVIYKPLWEEPHGYGAGWKQNLARVKEVLPEYLHSELSFPLNTLLMFYNKISVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSG1</accession>
    <name>A0A1E3NSG1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020210.1">
        <property type="nucleotide sequence ID" value="XM_019160449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSG1"/>
    <dbReference type="GeneID" id="30177136"/>
    <dbReference type="OrthoDB" id="10511474at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="629"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="659"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="778" mass="85791" checksum="36C032DCD9FA869B" modified="2017-01-18" version="1">MSSKNTNSHFLDSSPSKELYNDDSEMISLLNNMWGSNYNNPKNNPNAASSNPTNHADSVPSRRLTSSALSIAHDHLSKQTPHKTLANSRQPSIPTVPAHHINSQHHDSHLIFLDSPNFDSFITFTPNQIQPSNKLFSTQKQHQHQHQHQHQHQHQYQHAGHGLAKELSKSQLLPAQYMPVENFVSISPSLRTPLANKSNVEDLFDTPYLNTLYSTFANTTNTDELGNQNSQQLHSQPQHVTEIAFIQTPLTEKLDKIFKTPTSNDIFNSKQFFGNSTIKRLTNSYIPSNTNSAMKVNPHGYLVDESHTGAMNRGTSGLVNNNTDKIIETDFANFKNSPCVKHKQYEMIQKEKMNMKSNEKVSNPGSNLLATVRESEESSKAVVQNFFQAPMALSQNTFNPEAKDPTSAANAKMLNMVEPTVTTPKKLKRSRSKKKQSNKASKRGSGDEAKNITPNRFGVMKGTSVISKTNVDNSMLENEGMIDSSPSTIVVSSASKSSTRLTVVPGNKTLEASPTPMNKYINSVLAAESVQQQAQLISTHIIGDISNHSHNDISSSTDNIVDDGDKNHLHSHHNEILIPNHIHNISSNHINNNLFVPNGQENPMLDVMPTAPVMGVFKEQSAKSLRKYKTQPLGSDGFNKSSLPTSNKKFGRSTSLNDSMSTGNLNSNSSSNGNAKGNNGKGKKRNRGNSGTIHFIMADPDQLKGNKKRRISRKKPDSLSSGKKNGDKFVPLRNSTNIVNNRNRSTSPVKEKNINTVSGGANINNTSGNENNVFWESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSG7</accession>
    <name>A0A1E3NSG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14158</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019731.1">
        <property type="nucleotide sequence ID" value="XM_019160250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSG7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSG7"/>
    <dbReference type="GeneID" id="30176937"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF73">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="218" mass="24829" checksum="256179AA69221E1C" modified="2017-01-18" version="1">MAMGGSDPASAHKCKISMLWNWYTIDSCFLARSWHVTSRGMFAGTCIGVFFWVMAYCWFHRFIVEYDNAIVDYKLKKANVSACESCCGAGGCDEILSSEEKGSSDEETSRAAAVAQGVKRPGSDFWTPLKNTFSHKWYLAWMKKDGNAIYPSPVEHVGRSGLYLMEWTVSYLIMLMWMYYNGYLIITMILGYFFGQLFFNYTPLTVVPRKDYTTPSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSJ9</accession>
    <name>A0A1E3NSJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cardiolipin synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_21708</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020137.1">
        <property type="nucleotide sequence ID" value="XM_019162220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSJ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSJ9"/>
    <dbReference type="GeneID" id="30178907"/>
    <dbReference type="OrthoDB" id="10020554at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043337">
        <property type="term" value="F:cardiolipin synthase (CMP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007006">
        <property type="term" value="P:mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000017">
        <property type="entry name" value="Phosphatidyl synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF60">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE (CMP-FORMING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23015" checksum="1FEC3BEFE2CACC3C" modified="2017-01-18" version="1" fragment="single">HENIYTVPNALTFTRLVSAPVVGYLILHGQVTLALALFTYSCVTDFLDGFIARRWNLQSVVGSVIDPLADKMLMMICTVCLATTSEIPLYLAVIIIGRDVCLGLAAVVIRYLSLPAPKTFWRYWDFSIPSAEVHPTMISKVNTALQMLYLGSMMIKPVFLLYLSDHFGAETTTAFLQYTHVLEVTVACTTLWSGLSYAFSKKAVKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSN7</accession>
    <name>A0A1E3NSN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_70646</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020183.1">
        <property type="nucleotide sequence ID" value="XM_019163694.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NSN7"/>
    <dbReference type="GeneID" id="30180381"/>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059070">
        <property type="entry name" value="TPR_VPS8_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25066">
        <property type="entry name" value="TPR_VPS8_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23413">
        <property type="entry name" value="zf_RING_Vps8_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="3">
        <location>
            <begin position="576"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="VPS8-like TPR-like repeats" evidence="4">
        <location>
            <begin position="1146"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1393" mass="159496" checksum="BD7277F287758D46" modified="2017-01-18" version="1">MSELQTQHYVEQTISPNKQFDQRLSSRISTQPQSPTKSRRQPNDKRLRQDLESGSCNSLLRPEFSLSKLQWLSLTKITDQIYSSSSLSENGDPQLLAISDEYIAVVTSKCYALLFSFQQVLLLKVKWANITPSTRITALALSIDSTYLAMGNSDGAINLYDLKKADPIITITPTTAHELQFDGSHHHIAHLKGSAIRFISFIGSRHTGFVSSDDTGITIFHNGGRSLIGYSCRSKIVFGRYDLANIMTGKVNYSNTVLDFHLLPIGSKKSLADEMSLISMITPHSMVVLSLNSELKTHIKMKKPKISDQSLGMSGCVSWFPATVGDFGRHENSPMLAYAWSNILGVLEIKAENNTNEEGEQTVILNVTSKKETKYSETIVCLRWLNYRVLVALTRSQKLIFYDSSSLVILKEVDLMYKQMKSTPIFPHNSIGLIQKSFFGSFFTLKSSIFIFQHREIAVANISNWADVLLNLLEKGKYIEALKESKRQYQGGEDNPLLNLPDDDEARHSIMKEYLIQIFRSSLKYIFTEQSSSDNSLDRYREITLLLIQTCLTINAPSEMYDLIYEKLLDYRLDSVFFQVLENFIFSSEIVTFSPTILKAMVVHYIEEKDTNTLERLICLLDIQQLDIDLTVTLCKQYHLNETLAYIWTTLLHDIFTPLIEAVLRIKTFNEKENVLSDAEKEEIVNDIGYVYPYISYTLTGRQYPTDKLFPYTYSLSAKLNVYYFLFNGSTISWPLGSAKIHTVDDYKAEPAFPYLSLLLKYDSESMLSCLNEAFEDDLLNDNEMVGSGSTIDDKYQLRVSRQYIIDVLLGVFHDNDSSFSLADNIRLAVFITRNYPKYLQFIRIADSISDEMIAILCKAGTSKKNINITTELVQDCELGLQSLLSVYKPLDIDYVLLQVKKAGFYQVLLYLYQSEERYMDVLELWVDLQNMKKIEPEKTGNIELFKPISEILKDAMKASKGSNRAEIVQILSSNFELFVKSDPKSLASVVCEFCPELNSDVVNFEDNHIKFDYLREIFNTRDEGGGKKNKLLGAGLRYEYLKSLISQRRMNVSDISQSDAVLQAQIYDDPISEKSFMDNRIEHFVLSLDNLSTDIVDLLKSDESNEFEVLIKWYVVKYQYGNAIDSVCEILERSSNEMELKGYSKQQETKVWRCINKVFSVLSTKDPTLTKKLEDSLALRETLILQIIEASVSILTKLTNASSKESRVEHKVVDTFKRVVQSIFTYVINVSSEDSSSFNKIFKKFLDDSAIHQTKLGDVKMVLREIFISFCNDQQILTIIQILVDNDIFDNLMVLESLKIQGWSPKNIECEACGRKIWGGKIGNSVYESWRDHKLKNFRSEKINENNEGISQIDKLNELYVFQCRHTYHRKCLENMGMIKDRDKECILCRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSP8</accession>
    <name>A0A1E3NSP8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_95803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020242.1">
        <property type="nucleotide sequence ID" value="XM_019165105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSP8"/>
    <dbReference type="GeneID" id="30181792"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="112" mass="12625" checksum="EB1406A582438B3E" modified="2017-01-18" version="1">MWRVSQHGKAWQNRAMPCGSEKCVAGPASELVQAEMFVTRARRQRVYVPDLCRKSLVADGLWSVGGWGDPLQVLDQPTPSCVPTWRTRYFLREGDESRQGRHCQPTQPAGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSQ6</accession>
    <name>A0A1E3NSQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020203.1">
        <property type="nucleotide sequence ID" value="XM_019160442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSQ6"/>
    <dbReference type="GeneID" id="30177129"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="55" mass="6313" checksum="74874D0CA6542700" modified="2017-01-18" version="1" fragment="single">MHGWAIKGQDVKLNPLVISPEEKKFWAGRVGLNYLIWTRSVLRVIVTSSAERCRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NST2</accession>
    <name>A0A1E3NST2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SOK1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019746.1">
        <property type="nucleotide sequence ID" value="XM_019163594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NST2"/>
    <dbReference type="STRING" id="763406.A0A1E3NST2"/>
    <dbReference type="GeneID" id="30180281"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="90101" checksum="AD1A1A7AC829BA32" modified="2017-01-18" version="1">MMNHLNNSNNDQDIERPPHFKNVFSPNDVSANADNCLNTNNIVTSESRRPSLVVVPMNNGGHDMRHLALTARSDDLNLDIEMQDGSTESDDASVGGGASTASSACSSSSSSAGGSQTASSSASSVPTASSATATQIMDIPPRSCTDGVLGKPDTLPAASTGNTPLNRTLQNENLNNEHSEKANSNPQLTHQLISNFQFLNLPSNKKPTKKSSSKISKNTTKPIKKYRSKSLPNIHIPSKRSHQILLDIYKQNFKNKNGRSKPGNAVGHNLNNPDMNMNMNMNVGMMNMNMNMNINMGMNMSMNINSNGTNNFMNIHHQQQLFLQHQQQQQMLLLPPVNLHSMHEIDLQEVLKNPQLRHDILFDPQLQFRPNLDGERGKRKKVQSENYWNMIKFETERLLKSSHPKAIDHNSPIIIMFQCLKSILVSLIPSKDISSIEEILDISLLIQELNSKCFNFLGFFDWICSIFKLHCAPMRDTWVDELGTLFHKACESEDEVNIDYLIEGLKTLFLILEAMKLDVANHQIRILRPLLCSSAVSFEKEYFKNAIKRNKINFTSSMLWFKKNSIELDSKNPRDVLNYSVLNLLSCSTMYHQFPNTLNFDHTRLLLLRADLRHIICTKLCSILYKTLIYQNNLDKSLLNDANMLSFKKEILNIIVDENGNSKWTKNLKNLSVQLINNLFGNLDSDKIDFAYNWLLKQTQPNSKVYSLLESKLFEKILPFLSAPFAINNNTNTNNNIDSISTSMATNSNSSNSNSTSSKIEDDIIVNDELRNVTDRLNQLIEFNYNVFSDMYTSYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NST5</accession>
    <name>A0A1E3NST5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome biogenesis protein RLP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_19010</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biogenesis of the 60S ribosomal subunit. May act as a specificity factor that binds precursor rRNAs and tethers the enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020318.1">
        <property type="nucleotide sequence ID" value="XM_019162102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NST5"/>
    <dbReference type="STRING" id="763406.A0A1E3NST5"/>
    <dbReference type="GeneID" id="30178789"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000465">
        <property type="term" value="P:exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF26">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="9">
        <location>
            <begin position="103"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="284" mass="33001" checksum="C3C1C7E9384E443E" modified="2017-01-18" version="1" fragment="single">TMSTTEKKLDSNPEILLKKRKNADRLRIEKQQQAKRRAEERQKQQSAKKSNNKFVRAETIVARRLATDREKERIKRVTKWDKAYYKDEQTEKSKNKDEEERLLFVVRISNPHMGDIPPRARKVLTILRLQKVNQGTFVRLNKKVEPLLHLINPYVVVGTPSLATVRNLIQKRATETENGFNESKPVEGHDYRIIALNDNNLIEEKLGDSGVICTEDIIFEIYKLGDAFLECVKFLEPFTLKEPVTGWGPLSKLQRLEKKEESKSYQASSKRNAPLNEVDIDKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSU2</accession>
    <name>A0A1E3NSU2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_9648</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020284.1">
        <property type="nucleotide sequence ID" value="XM_019165119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NSU2"/>
    <dbReference type="GeneID" id="30181806"/>
    <dbReference type="OrthoDB" id="4089405at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="384" mass="43666" checksum="73B137443662AF6C" modified="2017-01-18" version="1">MFHLIKHQSTSINSGNPGKGTFTADALFEERLKSIEEIANETYFQSETIGASSEERVLELYKSIQNYQTSIYDFDKLTLEQNIYLRRSNDLLKKLLVDPKTHFDEMLLKKLFISQPLFPTLKAIIKAYYSEGPSVCIPSALALIPFRKLIWDADFQDALDYVELTNGHKRYIENRKRNIKRIYAYFGGSIVGLIACIHGAVSVFFPELISAGAGGTSYGIYGIYACIVTYIVNCGFLAGLAFSSKGLENGNLMFKQSTMPHDWYLKVDQMKMCSKIVEADAEINGMDGFATRDVVSRIQKMGFEVNEPEQEIMLRQYWYSSGEGFLWVEPDIDPAEIEWWKHLDDIGVKKVWDQDLNKIEEADASEDVEDGDNGAELFISEDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT25</accession>
    <name>A0A1E3NT25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14690</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020335.1">
        <property type="nucleotide sequence ID" value="XM_019160486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT25"/>
    <dbReference type="STRING" id="763406.A0A1E3NT25"/>
    <dbReference type="GeneID" id="30177173"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF9">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="258"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="260"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="338"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="550" mass="60569" checksum="4C7C09439AAAD990" modified="2017-01-18" version="1">MSTAASVTETPLKHTAQGAVSLPPLSSILLSPAESSLRNSLIHESTPQRHKVPLLPARAKPNIFNTGGAKPPHSSRAFSLPSIESLTASPTLKKSLSCSASLPLPTPYHDSFYNKSFVPATPLTHKLPLAPILNQSTPLHLSRSRTNTSIFPVLSKQRNANVSLTSDNNESSFCASTPFKSTLNTSSLSSGSENIFSSKNIINSSTLKSDLSIKLQDKTPGSVSKKRKASLDESKSFAFISHSQETFLSNEPNIDNARLARRKRRRTSPTELAILKEEFKKGTTPNKQRRILIAKRVDMTEKAVQIWFQNKRQAMRKIQNQNSKEFVVEINSKKSPDTSDDEDMNEDEDAENDNDNDNDNNNDNDNGNDNDNDEHENEALLSVSNKLKTYTEAELKIASESGIYSDDSSVDSKQLNLLEENKENIDPDVKSPSKVGSVMSLPPRGIFSSPLSSTKSGKKMVLTDITNQQAGQTFKFKSTNFGLISHNAITSRRQKPTMKLKLKSGFANSSKPNEFKHQGLVILKDATNHSNTKSEIKLKVHAAPQLKSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT84</accession>
    <name>A0A1E3NT84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c oxidase assembly factor 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_21311</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020424.1">
        <property type="nucleotide sequence ID" value="XM_019162193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT84"/>
    <dbReference type="STRING" id="763406.A0A1E3NT84"/>
    <dbReference type="GeneID" id="30178880"/>
    <dbReference type="OrthoDB" id="5545577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048281">
        <property type="entry name" value="COA6_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47677">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47677:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10458" checksum="5FECE65FBE326AEB" modified="2017-01-18" version="1" fragment="single">PSRSKREQCWASRDIYFKCLDKNQIDNPLDPSKKKLASKECGEEDKQFEKDCIASWVKYFKEKRPFDLKKERLLKEAAEQGQEVVQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTG2</accession>
    <name>A0A1E3NTG2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020457.1">
        <property type="nucleotide sequence ID" value="XM_019160544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTG2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTG2"/>
    <dbReference type="GeneID" id="30177231"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="645" mass="73914" checksum="029BD8527F2C45C5" modified="2017-01-18" version="1">MAGPLLPFAYDQSRQDENLPPLNLSQPRQKDKSTHDTDDIGGNFKVRESTDYENDSDTYSDEDLEKENDFKPKPSKFLYSMINSSSSVLVENNRPLSDFNNFTNTSSTILEFHNQNLSSDSFHQMDTSNIDVPSSPFHSQNVFNEKPEPLSSDAFDDLVSITNNNLDSKPSLPSSPTLLPLSQSTQLKKDSFTLDRTSKFHRPKINKQLHPLSRHGSTFTEPTRKKQRMVQERAKSIPLPPALTSNSNVEFAIDRDGHVYGYKLEKDELPQNYLPTLYDEPIYFVVKNSPIQEQNLLKAIDEFFEQPVSSTKPISTLNLQYFNLSSLPDYICDIQNFYDCADDGIVKPLIHIDASYNKLRSINPKLLTIERLEMLSLRNNKIARLSGNIDKAKDLKSLNLAMNKFKFLPHNILNLRKLEVLATTGNPMISQTSVDKFYTINFELLKLYSQQTGSVEFLDTDKQILSFSRIHWLKSNKQISKNAVHASILSRSLSTIQDLCYDDNGNLKDWNDFESSNKLKLRKKKQSFVESKLTWCPKLTELALRQVSKYLISQSEVQKWKDSTSEFIYKRAMNSLIYGTNGETCGNCGENCIESVADMLEWWEFKGSKSVTIKRRFCSKHCATCWVEKLNKFKLADKRLTTDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTS7</accession>
    <name>A0A1E3NTS7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_70942</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020509.1">
        <property type="nucleotide sequence ID" value="XM_019163778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTS7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTS7"/>
    <dbReference type="GeneID" id="30180465"/>
    <dbReference type="OrthoDB" id="6129702at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140278">
        <property type="term" value="P:mitotic division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052557">
        <property type="entry name" value="CAP/Cytokinesis_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333:SF2">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="3"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="758" mass="86892" checksum="3668BB15036A72E8" modified="2017-01-18" version="1">MLQLPIKTRATYSWGGNKKTNELGFMEGDVIEILKVVNDSLYFGESQRTKLRGFFPAKYVACNVPNENQTSPSAPSTPVSANSSKNNSFNSLFTLKKKDKSSSSTLNTDILNSSFQEKNLSVVASPSSPKNSEQKQAKKFTSSYVQQLLDASTLSTETGSSSAFGHSDFSATSAGSYMRHKEREEYESKLQSMAHSTHTSIATRKALDEIIEKNEKRKQPKLLRDLLGSRNDTGPSFDDRIYLMSVEKMSQMELDEGLPYGYQSFPSKRFAEPISDMERTRTVSGHERAKRAKRILDEEPELILKPQETISHVNFEETDTSSRSCSYSLDSEGLEKVDHFMSQQEHNPFDTPQKFTIDTILRKFKTELEVSRAIYTYLTQSFKLIKRKDEKVSTKRMFGSEKISEIMHTCLCTPHQLAWLFYIMADTAGLEVEIVLGYLKHPFALNDVVTNSKKRLIINHSWISIKIDGAYRFIDVILGNPTNDFSVNYPEIWDNNMTWRFYFLARPFHLIHTHSPRYIDQQHIVPPIDVVAQLSLPPLYPSAIASGVSWYKYNTSMMHLRDYELYDFALEIPPDYLVHGKFKPYDSAFECVDSFVQVYQKNDVRIAHFQGIMSKGCPAGFVYVVGKNEYSKKWNLLLSIPCFHQGKWKPLEWMKRVPGLNGVDVYVKEPKINNLKCGEQLFDIKVHGKSDWIREVQQFYDGSLKLGLFPPNKQIIELNVTNNQFTGEINLNKAGYWRLGVLDVKFMRWKVIAEWSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTY9</accession>
    <name>A0A1E3NTY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase assembly protein CtaG/Cox11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_28569</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020622.1">
        <property type="nucleotide sequence ID" value="XM_019162546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTY9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTY9"/>
    <dbReference type="GeneID" id="30179233"/>
    <dbReference type="OrthoDB" id="1704689at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005413">
        <property type="entry name" value="COX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26330" checksum="9CE2FDCC27076FBE" modified="2017-01-18" version="1">MSLAEYQRLRDQYRGANDRYQRQTTIYYSLSLMMVFLAFSYASVPIYRAICQRTGWGGTPITDSTRFTRDKLSPVSTDRKLRVSFTAETSGALPWKFVPQQREVWVVPGETALAFYRAKNTGDRDITGMATYSVTPDHVAPYFNKIQCFCFEEQRLSKGEEVDMPLFFFIDPEFSRDPAMRNIDDVVLHYTFFRAKHDQQHEQLFGNQSKASEEISTTTVPANSASTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU47</accession>
    <name>A0A1E3NU47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inheritance of peroxisomes protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14566</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for peroxisome inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020196.1">
        <property type="nucleotide sequence ID" value="XM_019160436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU47"/>
    <dbReference type="STRING" id="763406.A0A1E3NU47"/>
    <dbReference type="GeneID" id="30177123"/>
    <dbReference type="OrthoDB" id="3980558at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005780">
        <property type="term" value="C:extrinsic component of intraperoxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045033">
        <property type="term" value="P:peroxisome inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024758">
        <property type="entry name" value="Inp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12634">
        <property type="entry name" value="Inp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="507" mass="56181" checksum="605221A19EA822E7" modified="2017-01-18" version="1">MVQERIKHTTLRRGAFPRMKAADDDKDNDKDKAKNKNKHTNSGNGNDASVYKAEGAEVLKLRNPFRKLMRSKRHGTSHHASMPGHLQPSVSTASTGSGSSTHSSRPSQGMKKDNRKTIFRLHESEMSVYKSAKDPGDAPAIATGSSIHHIIKNSKPSLIAQGKFQIYQMNSALNYLSCGSLTHPILPSCTIIKINTNTYIIPIKNPVRYWRLTLNTDDESILHKFESTVQPISKFKVDLLVDFTLSSPFCTIIDTSTAHTSSSSVTLKSFLHVDSEDDSPKTENAIDALTLHHPIPVRPNSASTTSLKSSILRKYNTINSNSSLNTSQVSFAEDMASTRKVSDIHAVSESTEIQKCQNIKSSDDLNGNTSPNLLVDLDFDSDLDLDLDLELDLDLDQDLDLSLKMDSLDMDSHLGVMSTNMKGFSKGKRSISASDLDDLDEVDLDSEELLCMWNNIGDGITKRYSLSFKAYDLKKPISIDVFDKYRRNIKSVLKSEGAGIPDNFLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUI8</accession>
    <name>A0A1E3NUI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020421.1">
        <property type="nucleotide sequence ID" value="XM_019163756.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NUI8"/>
    <dbReference type="GeneID" id="30180443"/>
    <dbReference type="OrthoDB" id="2117459at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047168">
        <property type="entry name" value="LEC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024554">
        <property type="entry name" value="LEC1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024555">
        <property type="entry name" value="PX-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12825">
        <property type="entry name" value="DUF3818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12828">
        <property type="entry name" value="PXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="286"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1266"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1266"/>
            <end position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1368" mass="153865" checksum="E21B8D6125D5BB56" modified="2017-01-18" version="1">MSHPYKAFMAEQHGNGTGNGNGADHPLSVQANRERRIHLSPVEEHYLKKSLIKNELISEVNSLSPDYSDISGLRRFGPPFVPADPQNFLRNPDSSTLDQIYAISEANIDIFRNQFPLLRFIFDNYFATFPFIKIHLDKLGPSLRNQSKFWLKIQVLFELFKGKKISNSNDRGTTSKRKLLLYKFQNLFMILFNSSIYCSQDPQYFEMDKERRGAYKKLGKFIDTAESESMEKDEKKKEEALSQLESDRKEGSYLLDMNISPANLLKHLDDIDDDEYINGWYVNIVGVSIESETKSTFWGSKESKFYSFILHVKTPDQHGWFIKRRYSEFTQLYKDLKASFPASHIPDLPSKDKHEVEMNLDDDPNTSSSLKNTVNSAVMHNFDSDMIDIDKESVDSLFANSIDSPKMPMSPSSPSHNYFSDSNADSINSEITGDTNASSSSGGLMKGSKLSVPGFAKPNFLKSPITSPLLSPKKAGSGLSNKNVNKVEKSFFNTFKKSAWSSTSSSSSLVSPISTSEDLSSGLGISSSSNHESHKRDASINSNISNGSVESAESIKTYHSAHTADNEDLSVGMTKMTIKEDKIVFPREILRQTLRGFLKYVLYINVCAKSAEVAKFLGDESRIVPLTTKDIRDIQNRINIDHLRTVQHYKFQSALVGIVTVLEKDVENLKTQIYNEGFSYIFARIKHFKTLHELCGYGSNDSSRSWLKTANLVGAASVSNQEPVESSDPDDAAAPLRGLVRIILLEIASTQYELLIGSDSALGTLKTIKRLHSVFPYRLIAGILRFTNPLMMVKRMIDVFTYQMPTVPGMSGGVSAIGSGIGGMMQGLGWSKKKKDADTEKEAFADELNHSSSNSSKKGRSLLQLIFSGMLGEDLRKLEKELIEVHDLLVSHDNTGKEYGAGETIVQRIDKYFQSDDLIVLHIKEMSQSLGIDIPTAILMPNNGLPDCDDLGSETINSILQDYSTVKKAEIEETMKQKVAGLKEKKNEEADGKKNPVHEKVAETELNSPYNLAKRYFFLQLRKYDKESLMELWNEPELMSVIKEVIALFLSPLIDLFKKAEVYKYVPIFARYIGELIEVCEVYASDYGQFGRSDVVGALVGLEEKYSEDVYRFIRDMYLNDIKSGQESGQGELFEGIVNWLNGVVSFLRFVKKERPDLMIDLNEMLNGMDLGQSDKLNILKVIGEVVAKAEKKRAVLEKMEADGDLQKLNVEKERRHDWMKMARDRKVDGKWDAIHDRVFKVGEQIAGESEGGIYDAVGLDGVDGADDLDTDSDADSNEFDDHNENTAGPRRPAHTRTRFRDYAWNAGEFLAGYYSHDWSRVDRNLLHTAYGGNEFMDGTECGARIAGVFRDRVQAVLAEYDARQRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUJ7</accession>
    <name>A0A1E3NUJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_99210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020431.1">
        <property type="nucleotide sequence ID" value="XM_019165145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUJ7"/>
    <dbReference type="GeneID" id="30181832"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="155" mass="17034" checksum="8596441F4549A46B" modified="2017-01-18" version="1">MLSGVLALDPDSKPSSQPFPRVQCNTSRPAPRLVLQIGRGRSLSRSMYNSNTMICYIGASTRQGVTAEGDSAIAERPWLQLTTQEIAAGADRCYPGASRCSRSRQAHTHAHTTHILHRCHSQARCTTLDTQNTQAATIVFHRYTVFRYGAGDCDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUR6</accession>
    <name>A0A1E3NUR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">N-acetylglucosamine-6-phosphate deacetylase</fullName>
            <ecNumber evidence="2 8">3.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_14788</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate</text>
            <dbReference type="Rhea" id="RHEA:22936"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="3.5.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="11">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.25" evidence="2 8"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020436.1">
        <property type="nucleotide sequence ID" value="XM_019160533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUR6"/>
    <dbReference type="STRING" id="763406.A0A1E3NUR6"/>
    <dbReference type="GeneID" id="30177220"/>
    <dbReference type="OrthoDB" id="10264777at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="12">
        <location>
            <begin position="54"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="235"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="407" mass="44587" checksum="CED8052357ECBFA4" modified="2017-01-18" version="1">MARYLHLTNCLLADNSELKEDDLFIDTVLGIIVEKPEGGFDSENIEVIDLNGNIVSPGFIDIQINGCFGLDFSTTFSGEKQRLEFVQEYNDSMEKLLKYGVTSICPTVTSSFSDVYNDVLHILGLKTRSPTKADSLGVHIEGPFISPQKKGCHPPETLTAMTDGYQSLFKRYGRDFEKYAAIVTAAPEVEGCLEVISEITKSNQVVFSIGHTMSDFETGLKAIHNGATMMTHLYNAMPAYTARNVGVVGLISATRDELPSEKIPYYGLVADGIHVHPSAIKAAYDANPNKAILVTDAMSLIGLEDGVYKRGNQSVEKKGHLLHLEGTETIAGSATYLLDCVHNLIKWTEIPIQDALATITNNPARSLKISKRKGFLNPGCDADFNVLTKTGKLKEVYKLGLKAHDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NVC2</accession>
    <name>A0A1E3NVC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14938</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020606.1">
        <property type="nucleotide sequence ID" value="XM_019160603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NVC2"/>
    <dbReference type="STRING" id="763406.A0A1E3NVC2"/>
    <dbReference type="GeneID" id="30177290"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF1">
        <property type="entry name" value="REDUCED VIABILITY UPON STARVATION PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="2">
        <location>
            <begin position="106"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="559" mass="62814" checksum="AEE31EF15FC699FF" modified="2017-01-18" version="1">MSLRGVGKALYRTPHQLFGQKTAEDILFKQWEHDIKTALAGLEYLKLENQKWKKFWISVITNFIQVIEIFRDLHCELEHAHGADGAVAEKPIDKNEEFTSVTIHELEQAGKLASIILERTTRLAEDSSDSFFAKCNEMIHTLKGAEKLITKRSHKKIDYDMKSNKVDSTLKGSIKTDKDKVKLESEQQKLTESEVIYKDLNDKIKLVMPEILANLSEFISKLTYKLYFSNLDILNFIQRNVEKFDRIHGIASDSKFLVYDDIIADFNGLYSQAQSKLENLSLLKEFRSFRNKNLTEKTVQGVNTVAGTVVDSTVNFTSTIYTKAAKPSQKLSMSLTSIKIDNPIHPYDKHGMFTMALDPLDFIKSADYAAQLSESDLKSMSPNYDTKNSPYAAYSEDNLEDEADAESKTSEESGVSEEKLSVTGTEWMKPLRNSTLNKISSPPTSFSQAATTDGASEKVTLNSSLNTSHPSTATDGKYVANSRVQSLSLNSKSETYKYVNVTMDNITKRIYLVVSTPEIDCAPVNVPKGKLNTTDFELRDYFMAKSSITANAFAAYSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDA0</accession>
    <name>A0A1E3NDA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_18550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015189.1">
        <property type="nucleotide sequence ID" value="XM_019162055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDA0"/>
    <dbReference type="GeneID" id="30178742"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="6">
        <location>
            <begin position="193"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="339" mass="37334" checksum="5A0D5591F7BF3AB9" modified="2017-01-18" version="1">MNKANVLLIGTGGVGTIVACGLVYNKKASVSVVVRSDYKKVKEHGWEIQSCDYGHLPAWKPDHIFPTVDAAAEVGVVYDYVVMTTKNLPDVVKPEDVAAPAITVGKTTVVLIQNGFDLGRPFIAKYPQNFCLSGVSHIGSHNHGGVISQTQHDKCLVSYFENPNLGKEEQEAKAKEFIDLYSNDKNSVSYFPDVKWYRYRKLVYNATLNTACALTRVDTGRLALSGTLEAVSIPAMHEVIKVAKADGVELPEDVINTVVHSDDGDWFKPSMLVDVEKGNPIELEVILGNLLVVARELKVETPTLDLLYHLLKATQFKLKEGKGLIDVPKERSISSKFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDF8</accession>
    <name>A0A1E3NDF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_60627</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015272.1">
        <property type="nucleotide sequence ID" value="XM_019163451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDF8"/>
    <dbReference type="GeneID" id="30180138"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="62" mass="7544" checksum="597E30A59AE0CD04" modified="2017-01-18" version="1">MQVVTAKRLLFCADDDKREWLLGRLRESWDFFCSFEYNRWQGRYYVQPQLMYANGKMPLNTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEE7</accession>
    <name>A0A1E3NEE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_18388</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Microsome membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015602.1">
        <property type="nucleotide sequence ID" value="XM_019161983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NEE7"/>
    <dbReference type="GeneID" id="30178670"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF150">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="14">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="120" mass="12827" checksum="0603265567BD03B7" modified="2017-01-18" version="1">MSKTFTAEEVSQHNTRDDLWIIYNNKVYDVTQYLDEHPGGEEVILDCAGSDSTEPFDDIGHSEDAHELLAGLCIGELTGGLKKKTKDAGASGAGSSDSTLPLVGLAVLILAIVAYFVLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEN3</accession>
    <name>A0A1E3NEN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015718.1">
        <property type="nucleotide sequence ID" value="XM_019164737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEN3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEN3"/>
    <dbReference type="GeneID" id="30181424"/>
    <dbReference type="OrthoDB" id="5572782at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="28"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="524"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="891"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="958"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1027"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="909"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="960"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="973"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1029"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1053"/>
            <end position="1064"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1064" mass="116442" checksum="9EE855A102E6BE87" modified="2017-01-18" version="1">MVFHWNDVSSACNSHATPDASGYGSSDMKQDRKDSKDFKDSKDSKESKETPDSSFNFGSSAISHYILYINGIETVAINGQQHKCTLDGLQPGTNYQIDLVAFNMAGFRSRSSPIYVKTSAKPIKDSNMQSLENPDNVIKCLLHEQERASLIRNDPFKASITNGRSRSGTIEGAHQNSNHNSNGSHHHNENTNSPKTKHPHLIDDINELKWILESGLEEVQSLMKSYKEAEQEFEEEEINLISARNEARERRKYEDNNRMNLRQEIKLLEEQRVKGTGRISADEKKIKERLKKIENYNSQIEKWRLEIEHMKKKREYLFKENPEVLKKLEQDIVTLNKEIFSSQTEVHEAEDELRDEIAQRKLLENQKVTVIQLFEEINKNINDVTGLLSAEGMESLNRLMELKPEWKEELLREVVEIDEKAESDWKALQQKEWINFNNMKNEIDEQNKLSLLKGAPNTSLGVNGVSAGISSNGLEGNGNATTATNTTNVNGYLYNSMPSSSNNTVNSYNNLAFNSNSMRSMIRPGVSSSGSFHGTEGLPEKPSLPNDSGNNTFNSSNNANIWPLNLSLDSSGRNMINPTTDSLDTTTVDMLLPQNLIDGEDFDQLFETQSKHQPNNEDPIMSPSLSVRGLNKLPASSALGNSTNSVSPNISQLNNFDVYQGGSLNGLVLNSTPQANKVTSLSAIASGNNQNSMMGNSAPLNMIGSPQSLNAYLANSDNGLSSNLLDLGQAATTSPFSSNAAMNLGLNGNTANGSDMDPSNVAFNLGSNIGESNNHYSKIFGSLNGPSNATLNDNGNANLDFSLSRARSTSFGSSIWSNGNSGNASNNGTGTWGSKNKAKGFSFLGAPITMSSTSAGGSQSGLGYSKNHTIMSSLKNDETNEKERLQVKDDLSSLHNDSNSDDNDEYHNGEQSYQPTSPSFLKSMMEKFGSSPTKTISNKTMENALEDLDDIVEDKLSKRTNSVSSNSNTTSSGTHQKSSSLGSSRFFKLSRKNSLISASQHSVGTNGSGINSNGLGVSSALLVEEEPTSNANGALNSGNSGGGFMGRKLSFAFKRDKDREKEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEY2</accession>
    <name>A0A1E3NEY2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015759.1">
        <property type="nucleotide sequence ID" value="XM_019161860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEY2"/>
    <dbReference type="GeneID" id="30178547"/>
    <dbReference type="OrthoDB" id="5406216at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031452">
        <property type="entry name" value="Kre1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17056">
        <property type="entry name" value="KRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133320" evidence="2">
        <location>
            <begin position="18"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="282" mass="27007" checksum="0DA9312F439F71A2" modified="2017-01-18" version="1">MKLSFLIYFILLAFAEAAVIDTSIVGAAVTVTTDIAATPTAPAAVTAATPAATAATTNPAAVVPTTATPVAAAATTATTPAAAAAVTTATPAPPAATTPTTLPPAAATTPTTPPPAATTPTTPPPAATTPTTPPPAAATTPATATTPHAAATTPTTATNPAAATTTPTAAATPTTTTRVGAAAITTATGTTTGTTGTTGTGTTTPLTTGTTTATPTHKTTSSAAPTIVWMTTTINSVPSVYSISWTQTFTSMYSSVAAVPKGAIGLGSIKGSVGTTRTYRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF39</accession>
    <name>A0A1E3NF39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Cytochrome c oxidase subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Cytochrome c oxidase polypeptide VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_60358</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome c oxidase subunit 5A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015819.1">
        <property type="nucleotide sequence ID" value="XM_019163429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF39"/>
    <dbReference type="STRING" id="763406.A0A1E3NF39"/>
    <dbReference type="GeneID" id="30180116"/>
    <dbReference type="OrthoDB" id="5778907at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00923">
        <property type="entry name" value="Cyt_c_Oxidase_Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.40:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.40">
        <property type="entry name" value="Cytochrome c oxidase, subunit Va/VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003204">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036545">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02284">
        <property type="entry name" value="COX5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48479">
        <property type="entry name" value="Cytochrome c oxidase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="135" mass="15989" checksum="457BFB24F26C0579" modified="2017-01-18" version="1">MFANTIRAAIRQNARLTIVPARQQLLNKKYSDHHEETFEEFSTRYEKEFDEAYDLFEVQRVLNNCFSYDLVPPPAVIEKALRACRRVNDYATAVRVFEGLKFKVENKEQYQAYLEDLKSVREELGVDLKEELFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFE3</accession>
    <name>A0A1E3NFE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_13506</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015411.1">
        <property type="nucleotide sequence ID" value="XM_019160071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFE3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFE3"/>
    <dbReference type="GeneID" id="30176758"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF23">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="106"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="337" mass="38603" checksum="752AC542BF995FD8" modified="2017-01-18" version="1">MCHFLYGDKAAMLAFLVISNVLWFSVLVSWVFTFIRGPGIMPCRIPPYELSNFIHEGEKFKNVKNTDVSDEKSDISSESSLTVPLNRNVIPPPDIFECDYNGLPFWCSECSTLKLLRCHHSSVKDRCIPMFDHFCTFVGSTIGKDNFGFFLYFLFSMQTLMYFTCISIIVYSGVWGHLHAALIVFLITTGFMAILVGNLAFNTVGDIYLGQTTIERLQRTRTKKKIAKSQSNENDQTDLGEYVNVQHPTNRKLRVLVKLLPSDKPYNNGFRKNLRLWYTDFTRLGSPQQILMFQNSMFSEKFKESISERINSGNYVIFGSQSKLQSSSPSHLKIKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFJ6</accession>
    <name>A0A1E3NFJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18569</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015226.1">
        <property type="nucleotide sequence ID" value="XM_019162061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFJ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFJ6"/>
    <dbReference type="GeneID" id="30178748"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF416">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE GODZILLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="352" mass="36401" checksum="AFF16B8D402A814F" modified="2017-01-18" version="1">MSGERINNTLRNNGIAGGVFSFSLNDLLSGGSGAPQTDGGRTPGAGSIQFTSLGDTIASAIQGGTGAGGQQGGGQGPDIAAIINNIVQSLNTAVTDPHASHRRATQRALDALTPLRLDEIPAEARASNCPICYEPYVEYAGDVAAGASAAAPEEDARETPATPATPQRSAADEVLDGPWKGYDLSDPSISFPAIETATYQHTYSLAAEPDLGLEAELPAADAAAQTPAAAVAAADDDVHFAVRLPQCNHIFGRPCIVEWLQSNVSCPLCRREIVPELTEDTRTATTGLQQPGASGGMASERTYVYDTAITETYVPVDWTAPLSAGYVLSDPPLTLPVPGVGMSSGRRTGRDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFZ0</accession>
    <name>A0A1E3NFZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="4">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PICMEDRAFT_35634</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016178.1">
        <property type="nucleotide sequence ID" value="XM_019162955.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NFZ0"/>
    <dbReference type="GeneID" id="30179642"/>
    <dbReference type="OrthoDB" id="6666987at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.750:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000005">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000008">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00419">
        <property type="entry name" value="PurL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010073">
        <property type="entry name" value="PurL_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01735">
        <property type="entry name" value="FGAM_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003672">
        <property type="entry name" value="PRK05297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="21">
        <location>
            <begin position="45"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="20">
        <location>
            <begin position="216"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="481"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="22">
        <location>
            <begin position="697"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="887"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ45065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1360" mass="148527" checksum="755F94B324527B76" modified="2017-01-18" version="1">MASSTEKMLILPGSQALSNFRVSNLISDINSSVNSSIVTDVKSCHVHYVHTQGGLDEQTIEKLKVLLKYDNELDLSDEKNKLLNELVQIDGDHSDSAAKVEQLLDSDTYLIRILPRSGTISPWSSKATNITEVCGLGDKVERVERGMAILLKVRPGFPIIELFKKDQFACFASVYDRMTQALYINESVPKYNDLFEDNKPKPLVHVDIIDSKDNLIKANKDLGLALDEGEISYLVDAFKNILHRNPTDVELFMFAQVNSEHCRHKIFNADWNIDNVQKDMSLFQMIRNTHKLNPQYTISAYSDNAAVYEGSPAYFYGPNIEKKNTWSSTKEFVHTLIKVETHNHPTAVSPFPGAATGSGGEIRDEGAVGRGSKSKCGLSGFSVSDLHIPDLKQPWELAIGKPNHIASPLDIMIEAPLGSAAFNNEFGRPCITGYFRTLTTTVKNLEGKDEVRGFHKPIMIAGGMGAIRPQLALKSDFKITPGSAIIVLGGQSMLIGLGGGAASSVASGEGSADLDFASVQRGNPEIQRRAQQVIDACNSLSVDSPIQCIHDVGAGGLSNALPELVHDNGLGAKFELRDILSLEPGMSPMELWCNESQERYVLGVAQNNLELFKQICERERCPFAVVGTATSEQKLILTDKLLGTTPIDLEMSLLFGKPPKMSRTDFTKKLDLAPVDLAGVALTDALSRVLQLPSVGSKSFLITIADRTVTGLIDRDQFVGAWQVPVADVGVTCTSLGEELIKTGEALAMGEKPTIAIIDAASSAKMCIAESLLNIAAADIKTLDMVKLSANWMSSASTPGEGSALYQAVQAISMDLCPALGVAIPVGKDSMSMKMTWDNNTVMAPLSLIITAFCGVTDTSKTWTPQLQSIKDETVLVKVDLASGVPKSLGGSAIAQVYSQVGDTCPTVHDSKALKGFLTATIELHNKFDVLSYHDISDGGLIVTLLEMAFAGRSGLNIELNGKQDALTELFNEELGAVFQIRKSEYEQFVKIFESHGVGRNNISVVGQPVFDCDQLINVSFNGETVLNETRSKLQEQWSLTSYHLQRLRDNPQSADQEYALIADNNDPGLTYALTFEPSNDLGLKSLSSRPKVAILREQGVNGQQEMAWSFQQAGFESVDVHMTDIISGRVSLDDFVGLAACGGFSYGDVLGAGNGWAKSVLYNERARNEFSKFFNERSDTFAFGACNGCQFLSKIKSLIKGTEDWPSFERNKSEQYEARVCTLEISQDLNANPCIFFDGMAGSKIPIAVAHGEGRAQWESDEQMNKFVKNGLVGARYVDNYGVPTEKYPLNPNGSPNGITAVRSENGRVLAMMPHPERVCRLEANSYYPAEESKEWGKYGPWIRLFRNARKWVASNEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG21</accession>
    <name>A0A1E3NG21_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad9-like Rad53-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015406.1">
        <property type="nucleotide sequence ID" value="XM_019160068.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NG21"/>
    <dbReference type="GeneID" id="30176755"/>
    <dbReference type="OrthoDB" id="3997833at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013914">
        <property type="entry name" value="Rad9_Rad53-bd_dom_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08605">
        <property type="entry name" value="Rad9_Rad53_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad9-like Rad53-binding" evidence="2">
        <location>
            <begin position="682"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="934"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1211"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="937"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1287" mass="144946" checksum="E75233C4921AE78C" modified="2017-01-18" version="1">MGPIPLLDDTLTRKLPSMTFEKYIKKTNEGHANPVSESTSNSHHDLENEFGAAFELNLNGGSPALSKFQNPFEETPNEKDNNKNDIRLSMKSDVENSAIFKTPSKKETSTGKSTPAGSNRSKFLNFLQATSTNAAEEFRALKHKQLQDDGYGYDNFDNSNVGLVIDIRRNEDETGEETSMDDSSIRLNLIPTTSRYNRREAAEDTVDPTQADGNVHEEVNRLIRDQISKRSFEDEDKEYLIKDLNNSQKYKYSSSDVMDKEMSSEKTSNELQIHTSPSGREDHQNMFKDNDEAILKDTLDVTGSSPKNFDEGSSKTTQTFSNTTPVFVEETAMGGEKFVIQDECHDKYCNSQFTERAPESFELLSHSNNDKRMMLKETQVIDNFSQSIVIEEDEEEEEDEEAVSSAVGEYTGLMRKKANEDDEDGERIKTGRNILNTFKIDEESPSLSQSQPRYRRPSNPASSVSQGPEADHIQIQGSQANSSFGIPDTQVLSLPETFKIETDTNFNPGVEAVLSHISSPVDIELNIVHPMSENGSENENGNGNANGNGNETTDPEIPNTSAVSISKICLDIKDDAARDRNQEPSKATSTPIIENKRPLIVQVKDASTERQDENMSQTATSTSRSNIMERLSNVLKEDNQDESELVQRQLFRAERKRKENTYDIVDVLDCCCVFIMDSSSRIPGRITKMVQSDDVILVNVKVKDGEVIVDHSKIYAPICFSVGDPVKYSMDKRHNYVVTGLKKTHRSYEDMNEEDTYGRVETIDGFDKILIRKNSKTTKEVFEEIEVALEELYLTAPISRNYRYKLFNDPDDFQKYIDYQISRFAMLDSKKGNCLNGTGADDTLINLLPVSRNRSFMKSRNQGFFSNCLFVVTGIHSVRNGSRGDSKLNTPRHQRDKSDIQQLVEFIRLQGGTVLQDSGFEEVIKFKIREKTKGKKGNGGGIGGSGNALNISPRKAKKSGSDVNGTDARKAEREFRNFQCLENHDGCYVIDFRSRNSMYVDEEVGRFEFACVLSTRHVRTLKYLQCLCLKWPIIHTEFVKNCMKSEGFLRNWGNEWTKYLLISGESMFLNSSIGLDIFEFYGNWKQGHRLCDQVHLNRLFSGSDVVIVAEEYAAFERRLACEAGSSCGRKRRKTAAGDTDAAADRPGISDIRSEPGSLSDEETAPRRLVDGPASEETLLWIFRQLGFDRAVIPKQGWSVHHLLDLNHASSSTPGGSGARRFRQQGVRGDEAATAPDTHRKYVYVKYGNDIDRFRSALAAVDTSACVCTVNWEWVVQRIICNDLWFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGE6</accession>
    <name>A0A1E3NGE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Checkpoint forkhead associated with RING domains-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_17671</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DMA1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016295.1">
        <property type="nucleotide sequence ID" value="XM_019161687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGE6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGE6"/>
    <dbReference type="GeneID" id="30178374"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090337">
        <property type="term" value="P:regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000426">
        <property type="entry name" value="DMA1p Ubiquitin-protein ligase (E3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000030">
        <property type="entry name" value="FHA domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DMA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="559"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="699"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="92"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="274"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="753"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="31"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="753"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="805"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="831" mass="89660" checksum="2347218321AF63F0" modified="2017-01-18" version="1">MPFIPFSSANYRSSTSSSSFDQDGSNPILIGANANSNNTNSSSNANGNGTSNNSSNNNSTNNSPLFQQGSFPLRRKTSVGNALFSLVNSIRQPAMSQSSKVPRRNSQSSHSRSRSASIPLSKQASNPGSDRGHENNSSDDLNRAREEVESANPFPISLAPELIQRIETEGTSTRTNNSAPASRKGNKSDPQLTTILSGGHAAADQNGEEAELGEEGEEENRGEVNAEVVPMAQETRANSELNDYRRLGFQAMRTSGSYSSNNLDESVHNLNEIPRSIDHDSSNDGTNRNASASGNTNYGENGISITNGDNSAHNMFNPAAYNYSMANSQTIPNFRIYANGGIIGGDESISANGINNGTGNAVIATIQEDNGHTTSATNDFIAPPNNTRTRGNTLRDELVQYEPVVMNSENNHTMGTVINNPITIDDSRDDVVLIDAANGSPAILSEDDRAMQIIGTRGEERRDQSIGPHENNRDLRNRAIGVVGNEGSGRGLNSAGGTETATNTMYAQTTIQQEERDQSSCDPNGFYSIRLTPSIDHSSTHPYMFFGPIVRKLKPGKGISIGRYTERSKKAATAASGSSEPVVFKSKVVSRKHAELTVDERGRWYIQDVKSSSGTFLNHVRLSLPNTVSPRVFLNDSDILQLGVDYRGGSEEMYRCVKVKIELNYSWKKRAAKFSKEAHEKLKQLTNVLGGNKEELTPCVICLDDIKPCQAVFMSSCSHSWHYKCIRPLLVKTYPQFLCPNCKAVCDLEAELEDGDDDAGPSDDVEDDGGVDAVDGAAHSARAGLDLRPNPNGTSAGSGSGGDDYGSRRYEAMDEDLMRDVEEAAAMGAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGF6</accession>
    <name>A0A1E3NGF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016343.1">
        <property type="nucleotide sequence ID" value="XM_019164643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGF6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGF6"/>
    <dbReference type="GeneID" id="30181330"/>
    <dbReference type="OrthoDB" id="5595695at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="82"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="454" mass="49238" checksum="F5A969F51CD30A5F" modified="2017-01-18" version="1">MMVPEIDVNEGMDKPFEQVSKPWRFPAPTSYALTNATVIDTLKGVSIKGYTVLTQNGFIENVVPNGGFDSTKYKVVDCTGKFLSPGLFDFHVHMIAVQGETGLRDTLRLPQNVMLMRVGAHSRAMIERGFTTIRDCGGMEHYIADAINDNIIIGPRAFFCGKAISQTGGHADLRDNNLPGQAFDSCECHLSNLGVVADGPDACIKAARENFRRGASFIKIMSGGGVASPADQIEHVQYSDAEIKAIVEVAENYNSFVTAHAYTPRSIKKCVANGVKGFEHGNLIDAECAQLLVKNDCYVCPTLVTYKALASDQFSAFLPPSSKAKNAIVLKKGMEALVITKKYGVKICFGSDLLGNLTAYQSNEFAIRAKVLNPVEILQSATITPASIMKAGDVIGQLAKGFYADILVLDSNPLEDITVLDKPEKHLLSVFKNGLVYHTKWAEIKSDVPSTIAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGF9</accession>
    <name>A0A1E3NGF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenylate kinase</fullName>
            <ecNumber evidence="6">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenylate kinase cytosolic and mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ADK1</name>
        <name evidence="9" type="ORF">PICMEDRAFT_73975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <text evidence="6">Predominantly mitochondrial.</text>
    </comment>
    <comment type="domain">
        <text evidence="6">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adenylate kinase family. AK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="6"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016312.1">
        <property type="nucleotide sequence ID" value="XM_019164635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NGF9"/>
    <dbReference type="GeneID" id="30181322"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03168">
        <property type="entry name" value="Adenylate_kinase_AK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028587">
        <property type="entry name" value="AK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001380">
        <property type="entry name" value="PRK00279.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001381">
        <property type="entry name" value="PRK00279.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011100">
        <property type="entry name" value="PRK14527.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="8">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="6">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="6">
        <location>
            <begin position="158"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="41"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="168"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="246" mass="27091" checksum="07063C8C2A558180" modified="2017-01-18" version="1">MDQLKDLVTKLENRVAELETKTGLTTPLPPALRMVLIGPPGAGKGTQAPNLKEKYCACHLATGDMLRSQVAKGTPLGKEAKKIMDQGGLVSDEIMVGMIQSELKGNPECKNGFILDGFPRTIPQAEKLDSMLKDMQKPLQKAVELKIDDELLVARITGRLVHPASGRSYHKIFNPPKSDMKDDVTGEPLIQRSDDNVDALKKRLVTYHKQTEPIVDYYKKTGIWSGVDASQSPKTVWSDILKCLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGK3</accession>
    <name>A0A1E3NGK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Type 2A phosphatase activator TIP41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_60061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016393.1">
        <property type="nucleotide sequence ID" value="XM_019163411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGK3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGK3"/>
    <dbReference type="GeneID" id="30180098"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41441" checksum="4B85F3B906308B18" modified="2017-01-18" version="1">MPSDTPPHKNSIDAFHINSAREFHRLTTGSRFPSKPLPPAGTAIRDAKPTPTTPKQQQSSPNTVPCSHNNPVCCHCGQVVIPAPVASLPVKDSPSICINNQWYICTQRLPILNAQEIEHFEEILTDIPVPEMIFGNNKVEVLLKQQTSEDIRFHIMFNPLDALKLVERNPEDLIQVSYADSWFKSRQLKHQNSENVSLEVYKPYDWTYTSRYKGTMIQADDGVCWIQDLDGSHAIPLDKLTSNNPIKFYDDMILYEDELGDNGISMLNIKIRVMSNCLLLLQRLFIRVDGVLVRIIDTRLYVDFDEFLIIREQKFMQDHYNDLVNKVAGPDPKKMMRDINWCSTKLPVVETTREYIVLDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGM3</accession>
    <name>A0A1E3NGM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="11">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_36556</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11 14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11 14">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="11"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015831.1">
        <property type="nucleotide sequence ID" value="XM_019163016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGM3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGM3"/>
    <dbReference type="GeneID" id="30179703"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF21">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="11"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="96"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="312"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ44718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="411" mass="46099" checksum="D2657ED14A9A44A8" modified="2017-01-18" version="1">MAFQKIQVKTPVVEMDGDEMTRIIWKMIKEQLILPFLDIDLDYYDLGIEYRDETDDKVTVDSANATKKYGVAVKCATITPDEARVKEFNLKKMWLSPNGTIRNILGGTVFREPIIIENIPRIVEHWEKPIVIGRHAFGDQYKCQNIVTPECGGQLKLVFTPKDGSEEIVVPVYDFDGKGCALGMYNTEESIIGFAKASFALAVSRKLPLYMSTKNTILKQYDGLFKDTFERIYNAEYKEKFEALGIWYEHRLIDDMVAQMVKSKGGYIIAMKNYDGDVESDIVAQGFGSLGLMTSVLVSADGKSFEAEAAHGTVTRHYRQYQQGKETSTNSIASIFAWTRGLIKRGELDNTPAVVKFAETLEKATIDTVRVDGIMTKDLALARGETDRSSYVTTGEFIEGVAKRLTKELGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGM8</accession>
    <name>A0A1E3NGM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_169111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016382.1">
        <property type="nucleotide sequence ID" value="XM_019161392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGM8"/>
    <dbReference type="GeneID" id="30178079"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="117" mass="13411" checksum="5629B776B1F1307B" modified="2017-01-18" version="1">MKHREVLDVPIKNVMLGEKIQTLEIFSNLEAHPVLSCQISFSTFPTVAWRPSVSTSPELVYLPLLLSYFAKYHVLAQKDVSTFRGRSTSRSFFSCFRIFSLFLLHMQARVGTPKTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGP5</accession>
    <name>A0A1E3NGP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_74077</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016424.1">
        <property type="nucleotide sequence ID" value="XM_019164668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGP5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGP5"/>
    <dbReference type="GeneID" id="30181355"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000207">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000005">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="315"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="575" mass="64087" checksum="F7EACD9AD51785D1" modified="2017-01-18" version="1">MKYVVVSGGVISGIGKGVLASSTGLLLKTLGLRVTSIKIDPYLNIDAGTMSPLEHGECFVLDDGGEVDLDLGNYERYLGISLTRDHNITTGKIYSAVIERERKGDYLGKTVQVVPHITNAIQDWIERVSKIPVDNSGEEPDVCIIELGGTVGDIESAPFVEALRQFQFRVGHENFALIHVSLVPVIHGEQKTKPSQAAIKDLRSLGLTPDMIGCRCATALEKPTIDKIAMFCHVGPEQVLAIRDVNSTYHVPLLLKEQKMIHYLSKRLNLGELKINKDMQSKGEDLYHKWTSLTVQHDKSFEKVTIAIVGKYTNLHDSYLSVIKSLEHAAMKCVRRLEIVWVESSDLEPETESINKANFHTAWQLVCKADGILVPGGFGSRGIEGMIAAAKWARENKVPYLGVCLGLQVAVIDFTRNVLGKKNASSVEFNIDLPEEEQAVVYMPEIDRERLGGTMRLGLRPTVFQEGSEKSVIRTLYGDSTEVYERHRHRYEVNPKLVADIESHGMKFVGKDETGQRMEIFELDDHPFFVATQYHPEYISKVLDPSRPFLGLIAASAGVLDDICKLDLKQRDNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH32</accession>
    <name>A0A1E3NH32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17899</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016547.1">
        <property type="nucleotide sequence ID" value="XM_019161800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH32"/>
    <dbReference type="STRING" id="763406.A0A1E3NH32"/>
    <dbReference type="GeneID" id="30178487"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07500">
        <property type="entry name" value="HAD_PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01137">
        <property type="entry name" value="C1.6.1:_Phosphoserine_Phosphat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01136">
        <property type="entry name" value="C1.6:_Phosphoserine_Phosphatas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="358" mass="39423" checksum="C60F80F892A25035" modified="2017-01-18" version="1">MMRIDVSGIILIELEFTDSISCMVNLLESPIKRTMNYVITAIARGTEFSAADNAAISEVLKKLPIKLGSFSVLSQSKATDFIFDIDLTKVDHSGKPDESTLKDVESSIRKNLKTAEYSVPNVDLIFQKNDQHRKDKKLIVFDMDSTLIMQEVIELIAAQAGVEEQVADITTRSMNGELDFRQSLASRVALLRGIKSSTLWQDLKPQLTLTPGTKELCKVMKNAGCKLAVCSGGFLPLAEYVKEQLGLDYAFANLLETEKDQENTEILSGKTLGEIVDGARKRDLLVQLAEENGIPLEKTVAVGDGANDLLMMHRAGYGIAWNAKPKVQLEAPCCLNSGSLKDALYIFGYNDLEIEPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH39</accession>
    <name>A0A1E3NH39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_172475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016560.1">
        <property type="nucleotide sequence ID" value="XM_019161522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH39"/>
    <dbReference type="STRING" id="763406.A0A1E3NH39"/>
    <dbReference type="GeneID" id="30178209"/>
    <dbReference type="OrthoDB" id="3996734at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1189"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="698"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="745"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="874"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="899"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="929"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1217" mass="137928" checksum="6230A2A015743C33" modified="2017-01-18" version="1">MIKRSFINTLPLQLKSIILDDPQTQTSSSSASISTANEKVDKAELQPQCRGDVETDNLTDDLTSQTENLDNSSDLSTRSPSIFSFDSSILDNANDSSLGNNGNNNIKLDVKAKQFLVNKLLEQKGIIFTEFNDIQLKLFDVDSTLCPEEFDKLVAKLDPQSRILLCEKIKLIYGFNSLTSFIIRLINILNFSTFFSIFDITSNIKLYGFYSLTYMNPVEILVKQIRNDLFIVKSFKEFTIQEMKKEHDFHLPYKEFKRLNLNDGNLVVVKILFKNEQKPIDLSLIKPLLPKIKMLHLSLENESKLKPFNDLIDKYGTSLRYLKFGVNRGTFKKIPNKNNIDVMKLVNIDNLNFINDLKDCSSLDVFFDNWELRKNCDIKFQENFIIKFEIMQRIEHLKVLNIFHTYIDSKDLNKLKYFPNLTHSKISYVILDNDGFDMSECKNLQELKIHFTYQKSIEDISFNDDDNDNAVNYAENIHWPANLKKLSITIHNIKNLIQNFSIRLNILRKIAKPLPPSIIDLCIDISYDTGGSGPELYSISRDDILKISGLINKILTNNLENLKLKGISRYNYKIGKPLIFSAINFPTSLKLLSLNDFILDYNFINLPNLKKLYFEHSLFDSDFKLPVSDEILIEKCTFKDLSFIPSSSSKIEFKMCTFRKVPSYNGDVTNVVINNCRIMEQPTNNVFQFQQQLQMNTQRNNNNHNGNSSANGNNNNNGNAGSRKSNFSNVGHNNHNQNKHRKQQMDQNQSSMDDLQNHNITNASPQILPQRSQTLPFSSSSANSLSMINEYSNNMYQNPGTPQYSFANFTGGSLNQIPGLQSFSIPKQQTFDYGSANSLNRNSTDVVNLLNKDLEFQLGGLSLNSSQYLNGFANSTGTHTPSSNGFNNRIPSPQPLNVSISSPPVSSSESMGSNSHTGGHLPKRSNSFQQHSQSQHQQHQQQQQQQQQQQQQQHQQHQQHQQQHQQLQQHQQQQHQQQQQQQQQQQQQQQNFGMSNMMNQFSKSMMSPSMGSFNCDIDSELARSGLGNLYDNQNGVSSPGTNSYGYYNHTLTNNNMSGFSMMSNGMNNSDNGNSNSNNNTGGSNSIQHTSQADEEQYIMSPSPSNMSESRMSMSGGSASMLMSQHRQPSLQQQQQQQQQQQFIGSRPATQYFNPNQHLMNPVLGAMNSGGNSTNSISSLGVFSGLPMNSTSSQRNSFIDNEGASNHSMDVSGTHLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH73</accession>
    <name>A0A1E3NH73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73031</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Heterodimer with FRA2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016618.1">
        <property type="nucleotide sequence ID" value="XM_019164365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH73"/>
    <dbReference type="STRING" id="763406.A0A1E3NH73"/>
    <dbReference type="GeneID" id="30181052"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02984">
        <property type="entry name" value="TRX_PICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000092">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="111"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="250" mass="27446" checksum="4DC4607FE82D1804" modified="2017-01-18" version="1">MVLVEISDKDQFSELTSVKDKLIALYFHTPWASPCIQMNKVISTLADSDQYKSTTFLAINADSFPEISDLFDITAVPYFVLIKNGTILKELTGADPKEFVSALDTFGDSSQAATASSNADPTATVSTATPAPAPTIQQEETPEQLNARLEKLTTAAPIMLFMKGTPSAPQCGFSRQMVAILREHQVRFGFFDILKDDSVRQGLKAFSDWPTFPQLYIGGVFQGGLDIIKENLSDDDLFFEHALEEANSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHE7</accession>
    <name>A0A1E3NHE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dipeptidyl-peptidase IV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_73069</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016664.1">
        <property type="nucleotide sequence ID" value="XM_019164370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHE7"/>
    <dbReference type="GeneID" id="30181057"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="15">
        <location>
            <begin position="157"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="14">
        <location>
            <begin position="622"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="852" mass="97007" checksum="1D6F16BBC9F99051" modified="2017-01-18" version="1">MHSKEYMPLPGSQNTSFSWRYISLVSIVLVYGTSLLLSQVNSLYVNHKQLGLQRQLDYHPADKEIQGLAFDADAKAKRPLTFKDVRNGTFYAESKEIQWIQTPESLTNDSGDYIVVERGNYYLRSLQDSAKKTVLYDLGSSIEYQNKTYAVESVTFSNDLKIALLTCNKRHNWRHSFFAAYFAYNVETKDIQPIIASSTEENSKIALSVISPDSSKIAYVLNNNVYVNDVSDFFSPASRQITFDGGDQIFNGKPDWVYEEEVFESDRALWWSPDSSHLVILRSNDTEVPTFPIPYFVQNSPGNSSSYPTLESIKYPKAGFANPIVDILIYDLSNDTLKQLPASDPFYNDEEINNTDRLITEVTWVGNEQVLVRVTNRESDILKIFIVSCPLSDSEMSSIVSRFEDGRDDKSWFEISHNTIHIPKSEKRPLEGYIDMMAVDGYEHLAYFSPPNASEPKAYLTKGEWEVVDGAAAYDFVSDKVYFVSTEKSSIERHVYSVDLEGASKENLTDVSKDGWFDVSFSKGARYLLLSYQGPDVPYQKLLDLHKGSSEDFSVNTDLQKTLDEYSLPEVKFGNITLDEGLTVNYKETLPLNFDPQKKYPLLFFVYGGPGSQLVQKPFSISFSSVIAAELDAVVVTVDGRGTGFKGKKFRNIVRDNLGYYEVLDQVTAAKHWISLGYVDSERTAIWGWSYGGYMTLKTLEHDQGEVFKYGMSVAPVTDWKLYDSVYTERYMHTPQQNPTGYKNCRVGEAEGFKNAKRFLLMHGTGDDNVHFQNSMQFIDSLDVAGVENYDMYVFPDSDHSIRWHNAGMVVYDRLFTWLKRAFDGEFEDVYSLNAVKPNGHMDDRFFIDLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHE9</accession>
    <name>A0A1E3NHE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">37S ribosomal protein S25, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_20888</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016667.1">
        <property type="nucleotide sequence ID" value="XM_019162173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHE9"/>
    <dbReference type="GeneID" id="30178860"/>
    <dbReference type="OrthoDB" id="5542239at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016939">
        <property type="entry name" value="Ribosomal_mS23_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37799">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37799:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13741">
        <property type="entry name" value="MRP-S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029764">
        <property type="entry name" value="RSM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="240" mass="27701" checksum="F82EB1E100D6A8B1" modified="2017-01-18" version="1" fragment="single">ANQVLQRTSEYLSSGLVSSQPAWYKVLAHNTPRHAFNKSVRLEVLEKTKAEEVSAISHINARLADGFYVTKVKVQKKKDVSSLLKSQQLHFIEDDLRLLFYKQHPWELADPKNLVENENNLGNESFDWSHLRQFGKKLDGESVVQRTIYLMETEKKTLLDSYEQAKFEYYRLKIQDETEMNVVREEGEMFGAVYPKTAIEEGFDKETEVLQKWKTDAVEQTKILDAKFSNKDSSATASAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHK4</accession>
    <name>A0A1E3NHK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Imidazoleglycerol-phosphate dehydratase</fullName>
            <ecNumber evidence="4 9">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_59283</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="4 9"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016738.1">
        <property type="nucleotide sequence ID" value="XM_019163353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHK4"/>
    <dbReference type="STRING" id="763406.A0A1E3NHK4"/>
    <dbReference type="GeneID" id="30180040"/>
    <dbReference type="OrthoDB" id="447729at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000005">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="242" mass="26230" checksum="E0171D21F34E9850" modified="2017-01-18" version="1">MSVTEVARKKHKLEDDSAAEGVVRKHTAYIKRITNETKIQVAISLKGGAIELPSSILQKTYPPAADAKAQVISIHTGIGFLDHMLHALAKHSGWSLVIECIGDLHIDDHHTSEDVGIALGEAFHSALTSHGPIRGVKRFGSAYAPLDEALSRAVVDFSNRPYAVVELGLKREKIGDLSCEMIEHVLESFATSARVTMHVDCIRGRNDHHRSESAFKALAVAIRDAMTPTGSNDVPSTKGVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHU7</accession>
    <name>A0A1E3NHU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_144091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016833.1">
        <property type="nucleotide sequence ID" value="XM_019160360.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NHU7"/>
    <dbReference type="GeneID" id="30177047"/>
    <dbReference type="OrthoDB" id="3998261at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133362" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1261"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="687"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1431" mass="147147" checksum="CF4C805B268B96B2" modified="2017-01-18" version="1">MNLLSFIILQLLLRLTNVASLAIPNELHKKDDPCDPIVKTTVTTVLTFYSTGRNSTYASLQTTTAVGYTLNECAMAYLPTVTASSTVPSISTSTFTHCDDGPHCDKTTWATPVCPVGTLSAECNVQPTYIPQQNSYTVTVFTTSCGAPDGATGSFSCHASTALVGLNDVSSTFVVTNCDHADFVCPASLSFSSTKLVPSPSSTPPIPLTSPSQLTSSSFFVPATPTTVSSASPASSLFSSSTYVTQPSTTLSSSLYSKSPYISSVPSESSTITYVTICPGASEEVTCIIGSSDVFVGTTNIAIATTSCYAPPKFTGSFGLTPVLFTYTGLTTYSVITAFNAVVPSFVCPSSISSFLTSSILSSSAPTKLSYTPSSTFSVVIPSSSVHSSSFQHSISSSLPFSTYPSSSLTKLSSYTIPSSMIPPYTLSSSFKPSLSTISSFSSSQYKSYSQSSSYFGSSSSFTSALSLDSSSVPSLSHSQFSSQAPSSSTGYSSHKTYSSSYSQSSFSSQKLSSKILSTSSKFSLSSFPPKLASSSSISSKSSPNSSGCITSDVTSSKVSLTTLITGCDSLTGALCLKGISATLIKNTLVWFTTTSCSMPSGISGSFGPSTTLQAITTGDIVTTVTVTDYIVPSVICPTSSFPTTLIATRPYTRSTFTETESLCTSGSVIVPCSVTTSESLASNQQTVSSISRSPTPSSLTSSSKSPSVPVPLESTITVTTTHCKNGKCTLSTSYATSTFTASSSSFTMFSGSTYTTSFISKPLTSASPSVPVTLESTITVTTTHCKNGKCTLSTTYTTSTFTTYCSSATMSSGSTYTTSFISKSSTATLFTISTSSASSNTLATLISLTTPISSTFSTLSTFSTSSMTSKTSENTFFTGLTSITESSSETVHSISKSSTSDTSSLSVSTSATVTPKTLTSATFTSGTVSSSTTSALVTTPSTITAVTHETSSTASETIPGATPETVTISTTTVTTTHCSNNKCTITTSTIPIVSTQSNSTSTSEAKTNESSKPAVLSSSSTVPESDTASITTITTTHCENHTCFATTSEISIISNTASSSPASTVSSLKFSETSASVSYSSILTTLTTTVTTTHCEENKCSLKTNTLVTTLVSTVECSTSSASTKTDSATIPQTTMTPISTTSDSTAVSSTNVTSVQTSSPSIVISSTVGTPEGTTFSITTFTTTHCEDNKCTTFSSEIPLVSTTSTTLDSLASSLLTSTASGSTYVVKSTTTLTTTHCEDEKCFLSTETIVTSTTATTECSDSSETSLQPTELSSTSTSTSTITTSHAFPTSPVSVSTALSTTLVTTTHCEDSKCITTTTTASSSTASTVTSIITSSTGLTDSEEGTSTISKTLLTTLTKTSSGSSSAIVASQITSPSSYSLSTSSHTVSVPAASGSSSYFENAAGAVVTLPQNGAVFGMAFGLLMMMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI47</accession>
    <name>A0A1E3NI47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_73306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016920.1">
        <property type="nucleotide sequence ID" value="XM_019164443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI47"/>
    <dbReference type="GeneID" id="30181130"/>
    <dbReference type="OrthoDB" id="3991133at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="260" mass="30618" checksum="8680BDB91D06DF83" modified="2017-01-18" version="1">MRQVQRGVFLTSSKKLLYAHSPLKRAYPPDGLTVEKLLTDSHTRRERILTIYRNLIRLSAYLQFHTYMKKVFCELVRVKLRQDYEYRKAILLPGGKIRDVDRTNVDGKDAENAERRLCERLVNTVNFVHNAALDSVANETARPVSMEFKILSSILQYEVSKALPSGLKGKLQDNFISMEKFMEFRQIERSWNMLGVEEGRRENLWWIDNGMMKNEVCFEYLEELKNLKTNQKTFMNKMAGSILDFERYLVFLNEESKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI53</accession>
    <name>A0A1E3NI53_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_73317</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016933.1">
        <property type="nucleotide sequence ID" value="XM_019164447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI53"/>
    <dbReference type="STRING" id="763406.A0A1E3NI53"/>
    <dbReference type="GeneID" id="30181134"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="114"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="476"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="746" mass="85160" checksum="DB25E46F33F22892" modified="2017-01-18" version="1">MTAADEEHKSLAELLAEEYSGQTKEQREGNRFDKSKLKKLQLLIDQSKTFSQIISASLIQKQTHAEEPAKKKQKPDTRKTSSADAHSLDTYRQPKSLVNVTLKSHQLHGMNWLISLYQNGLNGILADEMGLGKTVQTISLLCFLLEQGIEGPFLIVAPLSVVSNWTLELSNFAPSLSFVPYVGDKKHRQKLRRKSLRKFNVVVTSYEISLIDFPYLRTISWSYLVVDEGHRLKNANCMLFTKLKALQVSNRILLTGTPLQNNLAELWSLLNFILPDVFHDFATFQSYFNFSEFESLVEDNDENNSEFNSLVSMEVRKALVNNLHTILKPFILRRLKNEVLTLPPKREYIVYSNLSPLQSFLYSTMLHPENDFRSTLLKTFFPLYVKYNFPELEKHVEQYLEMTDSAKSKKGLTVKTVQALNTHYEFVNSQIKSKRISNPLMQLRQLCDSPYHYFFPWNDDTKITSDLILNTAKLQSLDSLIPALINKYPREKILIFSQFKSTLEILQTYFDEHPSNDKVYSSKTNVFPLKTCILTGSSSKEERDDAISTFETDPDYKIFLLSTRAANLGLNLTAATTVILYDSDLNPMVDLQAIARVHRIGQTKPCLVYRLATLATVEEAIWERVIKKRVLEGIVIELGEFNGSDQWVTSDNKKIKLKKESVLSSLLNFYSSRRGKSGLNHYTDPSQLVTTLKSNSLTAEEIEEATSHALEKYEEGHRINEAKLSHIKLLSESDVTGQSALDDVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI98</accession>
    <name>A0A1E3NI98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_17107</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016957.1">
        <property type="nucleotide sequence ID" value="XM_019161468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI98"/>
    <dbReference type="STRING" id="763406.A0A1E3NI98"/>
    <dbReference type="GeneID" id="30178155"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23283">
        <property type="entry name" value="beta-trefoil_MIR_PMT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="635"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="331"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="394"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="463"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="752"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ45844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="791" mass="90147" checksum="7AB328CBC88AD391" modified="2017-01-18" version="1">MAPKRSASKPSKSQAIETSEEKTSELILEPGYMRQYPKTELSDEVSANRLVHTSKERLLVGSLIIFALVIRASNLSNPNSVVFDEVHFGGFAKKYIKGAFFMDVHPPLAKLLFAAVGALGGFKGDFAFETIGDVFPPNVPYILMRQLSAFMGVGTVLFLYFTLRATGCKQSVAFFTAALLVIENGLVTISRYILLDSPLIFFIAGTAYFYTRTEISKPFSFDWFRYLALCGTFLGFAVSSKWVGLFTFAWLGLVNLIRLWFFVGDLSVSVPSIFRHAAFRGAVLLIIPAVIYLFSFYLHLMILTHEGDGAAFMSSAFRIDFDDTTVPKSTSADVGVSSIVTLRHLGTHGGYLHSHDHLYEGGSGQQQITLYPHLDENNRWIIELYNVTSEPFEFVPITDGTKIRLKHMITSRRLHSHDVRPSVSEIEWQNEASCYGYEGFDGDPNDDFVVEIVKDKSVIGEAQEKLKAIDTVFRLRHAMTNCYLWSHETKLPKWGYEQQEVTCATQGIEPLSLWYIEDNVNIYLPLDAEKTGYKQLSFFQKVAELHKKMWHINNGLTASHVFESRPSDWLLLNRGISYWALAPHQIYLLGNPIIWWPASFLFLGYGIYIVVLIFKYQLGYNISISENSFNFNYNTFIFLLGWFIHLYPFFLMERQLFLHHYFPALYFAVLALGHLFQLFSSTFKNRKTYVLYGLIFSASLWCYYQRLPLTDGSDWTLEKCEASKWFSSWDYDCNNFPYENAGNNRPANADQGGIDFNSALKPQVEGFPPQDQQPLVAEPVEQDVALEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NID2</accession>
    <name>A0A1E3NID2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_27324</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017005.1">
        <property type="nucleotide sequence ID" value="XM_019162492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NID2"/>
    <dbReference type="STRING" id="763406.A0A1E3NID2"/>
    <dbReference type="GeneID" id="30179179"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000011">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="23"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="452" mass="47700" checksum="D61C20DB05269B6F" modified="2017-01-18" version="1" fragment="single">LEKRDDGDSGYVSIELQNENTFYLTEIEVGNPAQKIGVLVDTGSSDLWVVATNNSYCQSNTGGPLAINCSTYGTFDPADSETFHSNGTAFSITYADSTFAKGVWGYDDVVINGANVTDLSLAVCDNADNAMGILGIGLSGLETTFSGSVSASSSSSDPYQYENLPLRLKSQGLIKQVAYSVYLNDSSADSANILFGAVDHNRYTGDMIALPIINTMKSKGYNDALQLDITLNSLILVDRSSSQQAVIGTGAASALLDTGTTLTYVPQNVLSAVIGLLNAQYSSSVGQYVIKCADADNLYLVFNFQGQEINIEMSSFLVSLVTSRGSSSDYCMVGLQSSGASSFTLGDSFLRNVYMVADLDNMEIGLATANHDNSDSEDIEVLSTGIPSAMTPASSLTWGAQSTSLFVQSGVQMSSIPASQSSYQFQSKKSTTTATTKRDSNMTTGAAVTSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIF0</accession>
    <name>A0A1E3NIF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_12353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017023.1">
        <property type="nucleotide sequence ID" value="XM_019159918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIF0"/>
    <dbReference type="GeneID" id="30176605"/>
    <dbReference type="OrthoDB" id="3995514at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="266" mass="30785" checksum="FE58822061710904" modified="2017-01-18" version="1">MSASQEMMRFPSDVLFDQKLCRLQINNLRKTKPLVESECKFHVGHIHQDIQYQFHQIGLRLVEPEQDAAYDELIQKLFKQTGVIVGVPGLINSLVLECIKHNCSAPFLRDVIDQYDSNGEISGMRLLISLNRLRKGIWEYNILERVECIEENFLRWSFRKCVFEMTCLNEVKPSLYNQTTIVTRLLHKANYHPKLDQFASNSMLIFDLLHDEFLDNMTSPNDGVGFSFELVGPSAMLDCIKSLGLDIESGNHSYLEEDILKKRKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIF7</accession>
    <name>A0A1E3NIF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transport protein particle component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017033.1">
        <property type="nucleotide sequence ID" value="XM_019164468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIF7"/>
    <dbReference type="GeneID" id="30181155"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="26692" checksum="5569EBCDFA9B21D3" modified="2017-01-18" version="1">MKALSYLLNETVAHAVRSTIKSRSEDGLSGGRVFADVLGRVDFLKDATDVVAPSTLSYMNRIGYNVGFKTANILLMENTDATSLGNVSTTALVDNPLEAMKFICRDVWKNLFGKQMDNLRTNHIGTFVLIDNKPVPYASCHFYEGSAAVIDNSGSTSTLSTPRNMTISSFNSLPGVSETDQEQGQDTITDPTLLRASAYLEFNCGVIQGVLQCLGVGSGIASVSVQPEEEGRNGDARSVVYTVETRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NII9</accession>
    <name>A0A1E3NII9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_12899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016276.1">
        <property type="nucleotide sequence ID" value="XM_019159983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NII9"/>
    <dbReference type="GeneID" id="30176670"/>
    <dbReference type="OrthoDB" id="10364462at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="275" mass="31501" checksum="90E2571656D73ABC" modified="2017-01-18" version="1">MVYSFSKPKLVLRSTGRKVAIMSSVSSDSLAEFRKNLHFLLHSNDNVPSDTRNAKSVKTHGSQAAPRARRPPVENTVIPKKKIQFNDESYLGQKRWEREKRELHNTIDVKNNKILTLSKANLERAETIKVLTEKVVRLERLNSQKDAQIDQLEKVILRLQNLSRANENDMVTRNNHQEDDDYGEAGLEESVDLTPNHYDEEDLHESTISLLNSKNTLDLDKEINLNTQQLMDLDLRHLSFQRSSAQGGPPIYNSNATDPYKNDSDSDLENYLFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIV9</accession>
    <name>A0A1E3NIV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_34373</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NHP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017119.1">
        <property type="nucleotide sequence ID" value="XM_019162882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIV9"/>
    <dbReference type="STRING" id="763406.A0A1E3NIV9"/>
    <dbReference type="GeneID" id="30179569"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="9">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="98" mass="11544" checksum="2B74E418DCF96EA8" modified="2017-01-18" version="1">MPPKKERSRKKKDPNAPKRALSAYMFFANAQRDIVRAENPGIAFGQIGKLLGEKWKSMDAEKREPYENKAEADKKRYELEKADYLKRKENEYLEKHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIX7</accession>
    <name>A0A1E3NIX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_17319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017207.1">
        <property type="nucleotide sequence ID" value="XM_019161544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIX7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIX7"/>
    <dbReference type="GeneID" id="30178231"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="104"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="256"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="571" mass="63119" checksum="69AF0EC14D363A52" modified="2017-01-18" version="1">MPATGAPANIESLYTFKDDVSDPKLFETWKPKIQAYRDALDKNTLAEYRVPEPEVPKDLDAKIFDTVEFLSTTKALTDKEKEITTTSASDLAAKIASKEYSSVEVLKAFAHRAVILQQFTNFAMDFFIHEGLKRAEELDAYLAKEGKTVGPLHGLPISLKEQIGYAGKITNGGWVAWLDNIPKDDATTIKTLRKLGATFFVRTNQPQTLMHLDSNNNIIGRSRNPHNSLLTPGGSSGGEGACVAGRGSAIGVGTDIGGSIRGPAAFSGCYGLRPTSRRISCLGGVSSGKGQESVVAVAGPITNSVADIDLFMKSYINEGKPWDDDPWCIPVLWREVPEPSPKDVTVAIMYDDGLVKPHPPIARGLEFVESKLKAAGVNVVKFDPLDIQEAYDVVHEMYTCDGNYAQREMLRPSGEPLLPLTKWALSFANGDKMYNVTENRELNYRRDALRKKYLDYFTKEKVDFIISPTYVGCAPVCINDGEAGAYYWGYTTLFNILDLPTLVFPTGLKQDPKLDVKDTTYTPRSAIDKIEQDKYIPELFEGAPICFQLSARRYFDEEVVAFGKVIEKIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ28</accession>
    <name>A0A1E3NJ28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_25028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017258.1">
        <property type="nucleotide sequence ID" value="XM_019162375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ28"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ28"/>
    <dbReference type="GeneID" id="30179062"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF32">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="3">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="275"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="522" mass="59380" checksum="F2836C1A36FDA35C" modified="2017-01-18" version="1" fragment="single">DIPGQTISKSSPDGSPSYTGADRLVVVMIGLPARGKSYLSGKMVRYLNWLQINAKIFNVGATRRSKSKKVGQDFGPCNEPLPDDQPKDGNESANNDNSTIAQDASFFSPTNQKSIALREEWARETLDSLLDYLLLGDGCVGVFDATNTTVARRKMVFDRIMQRSNGKLKVLFLESVCNEQSLIEKNVLLKLKGPDYKNMDPVLAKQDFLNRLTNYEKVYETISEEEERTPNFQFIQMVDVGRKIISCNIDGYIASQIVYYFLNFNLNARLIFVSRHGESLDNMKGRIGGDSGLTERGKKYSIALEKFVREKQEEFNLKLLKECAQNYSAENDKVTVISSVLKRAVQTAEAFNDPVRYDVKQLRLLNELGSGNFEGMTYAEIAQSHPEEFEERIKHKLTYRYPGVGGESYLDVISRLKPVINEIERSKSHLMVISHRVVCRILMAYFLNLQKDSVAELDIPLHSVYMFQPKPFGVLWHLWQYDEATNSFQELDLNKCTDNLKTVKQVGISFRERKYSVVPTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ74</accession>
    <name>A0A1E3NJ74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DUF1640 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_59705</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017274.1">
        <property type="nucleotide sequence ID" value="XM_019163382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ74"/>
    <dbReference type="GeneID" id="30180069"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000214">
        <property type="term" value="P:regulation of L-proline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF1">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19292" checksum="F3BD8115369B7314" modified="2017-01-18" version="1">MKDGKFTEEQARVASNIIADSINDGIELYASNLISREILNKMSYKQKVDFAKLKGELQMIDRSDFNSIKNENERLRSELEKMRIKFKEEINKSLSSVRLDLSLEKGRIREESSIYDLKIKETDTRINQEIYNLKVQIDSVKTTVLQWLFGVCAGTFSLVLAYARLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ80</accession>
    <name>A0A1E3NJ80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72291</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017315.1">
        <property type="nucleotide sequence ID" value="XM_019164149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ80"/>
    <dbReference type="GeneID" id="30180836"/>
    <dbReference type="OrthoDB" id="5229455at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF43">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="573" mass="65538" checksum="B98E73D031EA1390" modified="2017-01-18" version="1">MAVQSGIVSAYSGVKPRSRLGCNGCKKLKIKCDEVKPTCSRCKKRGKVCVYSFEMVFQNQSLAENKKKVRIKDYELMQNDEKRLQRMALIKSTNTVSNNENELSTFHSEPMSTTTLGSFVLKSSGADVVPGDGSSDSGVHVSSGGSLPYRMASFIPESKIPLLPLPEHLLDHPYYKDAFNFYKYFTAHFIVAATPQMYRSNPFHKLIPQFAEEKENGCLLDLLVAYSLTHRSMVLTDENFTPHLVELLISRGIFRLVSSLNATTMEIKCEVVCVTALLMCTQKIFSGDDTDKYKETIDLCRKGFERFIDNDEKVSKLPNGKYQLTEEDNSFSYFLLSWIGYLEVIGMMMAISPKDFRMPYRPNPVFEKVELKKKSKIDLFLGFDIKFLEIFDKLIPILNMLEERHEVDRNSIPTDILSLALEWEHELNSAYTDFQAAPRNPDDPTESDRILNASNDAFYFTGLLHLYRRIYRIPRTSSVVQKMVQKIYDVFKNDIESASSAENCAILPLFVAACESISEEHRRFFYDRFQIQFLGGNFPAGDVLKILNDTWNTGDSWVNAVKRVRKEKGFFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJE0</accession>
    <name>A0A1E3NJE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_72345</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017375.1">
        <property type="nucleotide sequence ID" value="XM_019164167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJE0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJE0"/>
    <dbReference type="GeneID" id="30180854"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000014">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="11">
        <location>
            <begin position="132"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="571" mass="62886" checksum="B7D9959F1C6FB1C5" modified="2017-01-18" version="1">MFVVRRRAPSTLKVLSGGFKRCIGQKQGDEKQDSNDAKYLSTQTPIFKIFAVSSAFLSIGFALGFALADKKESKRSTTKLEDLKPLVYASKDRVGYALSKIAKIVGEEKITVMDSELKLHSQDPSKFIQAKTGEEPYAVVYPTTIEEVSKVVKVCYDACIPVIPYSGGTSIEGHYIPTRKGISIDVSRMDKILDIHKDDLDVVVQPGVEWTYLNEELEPYGLMFGPDPAPGALIGGILGTNASGTNAVRYGAAKDNILSLKVVLADGTVIKTRQRPRKSSNGYNLTNLFVGSEGTLGIIVEATLKLHVKPQNEVITLMNFKEIGDASRAVSELFKNGISCNAVEFMDNRQMTAVAEMGTGGERKWSPDHLLLLKLSAVDDSALNSILSRVENVGKSNNAFNIQVAKDKEEMEDIWRVRKTLLWNSLGWAKKFKPNAMVLPTDVCVPMSRLPELITKTMEKMEKANLLATAAGHAGDGNVHVLVIFEPEQVAEAHRIVNEMSTMAIELDGTVSGEHGIGVSEKRELLEKELGTDTIDLMRTIKFALDKKAIFNPDHIFKIDPSEKRHPFDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJI7</accession>
    <name>A0A1E3NJI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_11250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017349.1">
        <property type="nucleotide sequence ID" value="XM_019159774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJI7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJI7"/>
    <dbReference type="GeneID" id="30176461"/>
    <dbReference type="OrthoDB" id="2284405at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="151"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="328" mass="35644" checksum="2E6C8635A65D972F" modified="2017-01-18" version="1">MAFPLLLAFTLSRQPSPSRVTDTHLVFAPTSPAPPHSVTQLVQVSTRPCAHIPLTHCYVLLVFIVVAVVVVVLFSLPPRSIAALVASDAPLTRSILTSVSLVHFIFCAVSEIQESKPEPQPEQIIQSNPQDDDLDDDDDSKSNNTGANAKRERRKIEIKFIQDKSRRHITFSKRKAGIMKKAYELSVLTGTQVLLLVVSETGLVYTFTTPKLQPLVTKAEGKNLIQACLNASDEQSADDDEAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAHAQAQANYQQPQEIHYGNQQAYGTGHMNNGYIGNDQANYNYGDGTGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJM7</accession>
    <name>A0A1E3NJM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017460.1">
        <property type="nucleotide sequence ID" value="XM_019161136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJM7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJM7"/>
    <dbReference type="GeneID" id="30177823"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF15">
        <property type="entry name" value="ARYL-ALCOHOL DEHYDROGENASE AAD16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="344" mass="38792" checksum="9A12F044BEFD1AF0" modified="2017-01-18" version="1">MKFVNLGESGVKISQIIVGCMSFGARAEYGNWVLEDEDKVMEILKRCYDLGLRTYDTADMYSNGVSEQLVGKFLKKYNIPRYTVVILSKCFYPVDADTPGFTVDGLANKDPNNMKFINSSGLSRKHIFDAVTASTERLGTYIDVLQIHRYDPSTPKEETMRALNDVINQGLVRYIGASSMRATQFAQLQFVAESKGWHKFISMQNFYNLLYREEEREMIPFCKETGVGLVHWSPIARGVLSRPLDEMKESERMKSDMAMVHILKDLSAADEEVINRVEAVAKEINCKMSQVATAWVISKGGCPILGLSSVARVEEAVGALDITLSEEQIKFLEQPYKPKEVRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJQ7</accession>
    <name>A0A1E3NJQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_129016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017490.1">
        <property type="nucleotide sequence ID" value="XM_019159984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJQ7"/>
    <dbReference type="GeneID" id="30176671"/>
    <dbReference type="OrthoDB" id="3998289at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="650"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="661"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="739"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="850" mass="92298" checksum="86613E4FB123742A" modified="2017-01-18" version="1">MDTSDYTDLLDITDILNADPHTLLSFTDENTTLKSYTEDQQPSHSHNQNFNRTLTEPEAFQNYDAFYYSPQSASSIEPTRLNNGVPQAVNMNANADKSVLDEIAESKELDELAQYLTDHPQRNDYASDITNQDDSPDNANAPLTTNWYKNIYTHMNNHISFTTDDVPFQHSTKSSPGSPEASNIIDEQLMTEIDSLINKNVLIDDMNSPNTPNTERFFEHHTQAFAPPTLLLPDRAGKPGSARTNTGSINNNNNNNTFHNPHNSNSNSNGTGLHHNHQLVKKPSVKRLPSNVSQSPTTKRLARSPSKTELFTGMAQTAAVLATNSSSSKNLASLQRHESARAAARAVGNTFENLSSRAVARPTQTKTRSKPGSGGSVGGNGRTRLSNARSHPNLKAQAAAKSRNTHPDPPTPYTKSPSMNSIFDIDTMTLASSPSFLESPLRVSRLKSAKVKPSSSSIYSSATSASVRTNVSNGSCTSKSSRGSGTFDQYSQLPGSSETLETIVSMDTTASNDKDSQHQLTNYTSSNRLASSTSHPNLTYQSSLPPPPPLPLQMGSFGSTLSTKTDSSTASPTCAVYTSTPSPIAGSSNIMFTPPNLLTPNAANNYLNLTRAQSISSSTELDFAAPGASPLTPVTKTAIKFEQLSTSSPHAPKLSVWRTSPDAKRERERAQSVSLNPDRRHYINTTKFTNNFIEEPISYRSHKPHAHKPHAHKPHPHTHSSSAATSFAAVAVAAAPPRLKNPSHASRNSVHSVHSSTSTNSTISTISNPSTHDDRDPRPDTKKPATPATKHSKSSKDAAFKNLITTFNKKEPECYKPKVYSNMMQGLVEFQVKMGKSAKTAGPRAPRTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJR7</accession>
    <name>A0A1E3NJR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial presequence protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_72468</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017507.1">
        <property type="nucleotide sequence ID" value="XM_019164205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJR7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJR7"/>
    <dbReference type="GeneID" id="30180892"/>
    <dbReference type="OrthoDB" id="4953at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000015">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000031">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF16">
        <property type="entry name" value="METALLOPROTEASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07610)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="3">
        <location>
            <begin position="55"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="4">
        <location>
            <begin position="193"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1009"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="714"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1009"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1031"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1052" mass="119867" checksum="566F195E201C65F4" modified="2017-01-18" version="1">MVHSYFEQQCSFKVDYAPAVIKKWRSTRTKLQVTLIDQESPIVNGYFAVATEILNDSGCPHTLEHLLFMGSKKYPYKGLLDILGNLACSSTNAWTATDQTVYTLTTAGWEGFRMLLPVYLDHLFNPTLTDSACYTEVYHVDGEGKEKGVVYSEMQGIENQSWFIQNLENQRLLYKNSGYKSETGGLTKNLRTLEADTIRKYHADNYRPDNLCIIVTGSVEEKELLELMADFDSQLPSAPEIPSKRPFVDTPQDGPLGETIIKQVAFPDKDESSGDVIFSWIGPDSRNTVLDQAIEVLLRYLTSSSVALLNQRFVENDDPWASDTGYSTESYIRTAMTINFDSVSFDRLSSLPEKVLVLLKEHCDEDKLDLVRLRDMVEQVKWKFVHRCEKSPEPFSDIAIYEFEYGEDIQKDLVTYCKTLDEFDELMKWDISKWVDIFKKYFVENHAAIVIAAPSAELYEKNKEDNEKMLEDRKKALGPQGLKDLEQKLQKAQEDNNKEIPKEILARFDAPDPSNVKFILTESIGSGSNKDVTNKASESVSKVENDTPKDFPMYIHFENYESNFISIDLLFSSFEIPEKSLPLLSVFEELAKLAIVADDGTLVPYEDVVKQLKRDTIECHFGNSFNGRFKEFMSLSMVVKSQDYQKAIDWYLKLLLKTKFTKERVVVILNRLLKQLPDLKRSAGYMLNYLYNKHYYTDRSYLKSVEVIRNEDYLRNLLNRIEEEEFENIEKELNEIRESLFKLSNIRVLVFGDINKLDKPVSAWSKFVQAFCPSGKLTDIPYQEKVFSRIGHSKSENCYIISTPGIDTNLMRLITKTTTFDFTSDDAFKILLAAEYLQAVEGPFWRGIRGAGLAYGANCYDNPALGELCYTIYRGVDLQNCYTVAKTIVEGYASGQTPIDDSLRKGAISAFVNKLADSQSNYFDTASCQFYDDVLVKRGPNYNKKLLEKLSSITNEQLIEVFNKYFVNLFNPEKSICFISCNTAKAAEIGKFFTDQKYNVDVENVYVEEASDDSCDEGSESDDGSQGSSDSDSESDSDSESESDSDSESELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJV8</accession>
    <name>A0A1E3NJV8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_128760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017479.1">
        <property type="nucleotide sequence ID" value="XM_019159980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJV8"/>
    <dbReference type="GeneID" id="30176667"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="98" mass="10486" checksum="68D571D5787EE16A" modified="2017-01-18" version="1">MDRKVYDTETCMQRSTLARSMSSLLSPWAAAITLFQPLSAAPADASCAEDRKGSERVEKALPEAIASLQKGEGRGKPDALCQSACLPEQNTGNYKHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJY8</accession>
    <name>A0A1E3NJY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mtf2-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_26795</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC45 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017552.1">
        <property type="nucleotide sequence ID" value="XM_019162464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJY8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJY8"/>
    <dbReference type="GeneID" id="30179151"/>
    <dbReference type="OrthoDB" id="10258882at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003874">
        <property type="entry name" value="CDC45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043837">
        <property type="entry name" value="Mtf2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507">
        <property type="entry name" value="CDC45-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507:SF0">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 45 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02724">
        <property type="entry name" value="CDC45"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19189">
        <property type="entry name" value="Mtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mtf2-like C-terminal" evidence="7">
        <location>
            <begin position="110"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="494"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="607"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="769"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="494"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="617"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1005" mass="115186" checksum="E1FE607689CB8F40" modified="2017-01-18" version="1" fragment="single">TKIKENNVYKNFFQSFEKMKKDSENKARITSFEEQKSFKQVFDYLSKESNKIGVVKSLETFVNFQSPLQQQQQQQSAEDPNVSSKLSFFSTASGPINLETQYKNKAEARKKFIEALSPTLKYINTDIQKSSQMFDYVKINIIEAFLQNLNAKSGKAKTGSSTGLGRKGNKMRCSIEEIKQQSTSTPQHPVVDIQTLPILLKFCLNSLTFDFNSISTSLLLIDYIKNHESIELYGFGLNIDCYNAILIQVWSKTENLNMVGSLIDELKINAIQPDLYTFKILAKIYLHCMRVNDGLGSEPYIIWNDSSNEQHPMIITQNQFYREFERICKMSLTHSTCRLAIFVSCFNIDAICSSKMLSEYLKKQFIVFQLIPVVGYIDMKEKFMKLDSDIMNVILIGCGATADLESFLEIDFTEYLSSDMSAEEAQRLVSLGRLNEVKLTRKIYVVDGHRPWNLDNLYGSPMICCLDDNSAAELNTEKEAFYYLINIPADLAGDDDEEEEEEELDLEDSLDRKRNRNEHKESNRKKRKRLIDEHQETLESYYQQGSTISVPSSHQIYDLLSTLGEISIDFLWFAVIGSRSLRDVYHRVYDMVYPGLKNEMQRLQSENDSFSMRNDQASRSNGLNGSASSSSLLDFNVSKRADNTGLQSCKEYTLFLLHHWTLYDAFFYSDYVNSKCHLYSNDGRRLLKTMFARMGISLIQANQNWHYLDLDLKKKLDGLILREVGKFNLTEVIIDGFRRNYGFHGSISSGDYVEAVQALLEHAMPQPYENGKSKPILNNGRRRKNEGKKHDSGTKNEQFIAGFWRAYDALNNYESVIRGMEIAKFQQQFIFEKGSEIIQKGMVKTQSKFKVVILNESFTTNSSITRSNFNDSTFNKSASSLKDDRVVDIKTFGNGASMFQNPLILTKLGNWILNVQADLDEGLLPLLIGAYNSETSTYLICGLPKRRKQSKNNKVKQQKVILNAFSVLFEKVTQEISIQARMDSFQSAIIELRKEDLTKFLDGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK68</accession>
    <name>A0A1E3NK68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017632.1">
        <property type="nucleotide sequence ID" value="XM_019159809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK68"/>
    <dbReference type="STRING" id="763406.A0A1E3NK68"/>
    <dbReference type="GeneID" id="30176496"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18727">
        <property type="entry name" value="PIN_Swt1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049014">
        <property type="entry name" value="SWT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052626">
        <property type="entry name" value="SWT1_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16161">
        <property type="entry name" value="TRANSCRIPTIONAL PROTEIN SWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16161:SF0">
        <property type="entry name" value="TRANSCRIPTIONAL PROTEIN SWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21693">
        <property type="entry name" value="SWT1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="47"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="404" mass="46104" checksum="D8EA1E42A8D2017F" modified="2017-01-18" version="1">MDINDERLNDITNYVVDLRNNDVFIPTDLSSENELPNNNNGNTTRTAYLVVDTNFILSHLSLLDDLERLLHTKYMGTYQIVIPKQVVHELDGLKDADRTIDSRHSISRLARSAIDWCYLHFHESMPTVTGQRLHERIDKNALKDNAILDCCLYFQNVENGGGNMVVLLSNDKNLCVKALVNNVLTISYRLGMTADLIANNVVSELNNNYNFDDSSNQMPPEGQFNSNDSNVLMNNDDMDVEPIYIQENTIDLVPDGQDTSTPNSTSSPYFFHETSEEIFSQVTTLTLEAIKYAVESIFEDDIEMVGYDDSKMRTLLDAARCIVKMGLSTFSEFFDRRRFNPMKMLQDRGQMQIYVNVPKDVPSLKTFVAFWTDFLEGIYANRGQSQKAALSQIINHWQGLIKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKC9</accession>
    <name>A0A1E3NKC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gamma-glutamyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017684.1">
        <property type="nucleotide sequence ID" value="XM_019161203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKC9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKC9"/>
    <dbReference type="GeneID" id="30177890"/>
    <dbReference type="OrthoDB" id="2015213at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052896">
        <property type="entry name" value="GGT-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881:SF1">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="595" mass="64937" checksum="CE5965BABD1A2197" modified="2017-01-18" version="1">MEAPTNSRRSTVYSTKGIVSSTQPLANAAGIRILEKGGNCVDACVAISACLAVLEPASTGIGGDCFALFYKASEKKVHGINGCGRSAAELSIDWLKENYPDEIQPNHRLKADSVFKVTVPGAIAGWVDSIEKWGSGKVTLAEILEPAIDLCENGYIVSQISAHLWTLSESKLLRENKKDKNLKLFLPNESVSKAPKKGQFMQNSDLAKTLKLIAQHGKDGFYKGVVADAIVKELSKRNHVLSKKDLETHFSTFVEPINYEFLDHKLWEIPPNGSGIIALMALGLIKNLDKSGAIKLADLEHNSAEYLHLIIECLKISFKESEEYVSDYDHYKAKTGVDHNNVVDTILGDEYLSGRANEFKKDEVIENNKLVIPNPMFKSDTVYFTASDSEGNACSFINSLYEGFGSGIIVPDYGFILQNRGGNFSLNPKAKNSLEGGKRPYHTIIPGMITKPSTQSGDEELYASYGIMGGYNQPQAHIQVYLNMLHFGMDPQEALDAPRISLFAHPEMKHTDKGYGADGPASNDVTCIGIEPGIDIAVVEKLRKLGHSVEVISGDDRKLFGRGQIIRKESGPDSKHLVYAAGSDLRGDGASVPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKU4</accession>
    <name>A0A1E3NKU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017870.1">
        <property type="nucleotide sequence ID" value="XM_019164298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKU4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKU4"/>
    <dbReference type="GeneID" id="30180985"/>
    <dbReference type="OrthoDB" id="194139at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="743"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="88247" checksum="40BB041EF05D417D" modified="2017-01-18" version="1">MSDWEEDMYDDDEEEELSFEEDEAIDESKDGLDEKAKSFDEESLYFQAKNLKEEDNLVDAAKLFEVILSNQHATSTYVFKSLKQLIKVYESEKDLDKILELLTRLFDMKDDPHLTTLYFNSSLFKIVNRIDRENYEILLAKNIFLKFQQYIHQLSPEQQNETSNKKLKIKVQLCIANCCLNAGDLDQASGILSVLGNEIISANESIKSTYHLDILAAQILLLLSKKGNTLELKRLTETANNLISGIPQSRILGAINEGSGLVSMYSGDFKAANNYLQVAFKSFNDCGDKRRVHVLVKFILSSILSQSEVNPFQSSDFQGFIKIKLIENLMDMYAAFQNMDIYRFNTIVNRDNFVCNIGDYQIIDDFMPQLKELFYTNYIIEHIPVFEKVKFNYFTKKLNVTNLSFERLLIKIYNKGLIPNYKIDCVNNIIIKTSSDKFTEINSLQYLENAFKYYTLPNTNVAEEFATKCQQLGNDRTNSDKVKIVKDFPEMMDLDCEDLERLKYFDDDSNHAKENASSNIIDNNDIERNFPPLNKGFIPVNLDRLEFETRYNVESLQKIAFLLFSDHFIKMDLSSDSQIGLKLRPCIDKYIVLICNSIPSAVAKNVSYFDKIKDEKMQLEFSKLFSHSTVQSSGNEKNQSDQNIPDVLQTTLMNPIEPPSNPDVLNDADFLLKSKEKKILRLQTIEDFVSTVVSKHNEFKTRGCNFSTTIEGYDEDSGKFNRHDLEINRIVISRLFSRQPISADQSETTSLDNMSFDGAEDREDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL04</accession>
    <name>A0A1E3NL04_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol uptake protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_11798</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017945.1">
        <property type="nucleotide sequence ID" value="XM_019159846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL04"/>
    <dbReference type="STRING" id="763406.A0A1E3NL04"/>
    <dbReference type="GeneID" id="30176533"/>
    <dbReference type="OrthoDB" id="420606at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="61448" checksum="25E802ACE380D5C0" modified="2017-01-18" version="1">MSASNETNPNYYIYEGILSPGWIFGRKIDNSDSQYRFFRDNFVLLLGFVAIHKCLRVASRYLHISRLNFDLGFGVCFVFCLHGFNSLKILFHVTVAYGIAKSLRKRPTVAQTVLWVYCLSTLFLNDRYRMVQFGQVWGALSFMDTFRGLIPRWDVFFNFTLLRMLSFNIDYLKRHEAVERITHEIPLTKLNDKAKVSEEDLVSPAIGDLSSTTTSKPDHCSHLTERQRLEAPLPLSDYSYQNYMSYLLYTPLYIVGPIITFNDFIYQSKHTLPSITFRRNILYGVRLIFCFLVMEVILHYIYVVAVSKAKAWHGDTPFQISMIGFFNLNIIWLKLLIPWRLFRFWALCDDIDPPENMIRCMDNNFSAMQFWRAWHRSFNKWVIRYIYVPLGGSKYRILGSLAVFSFVAIWHDIELRLLIWGWLVVIFLLPEIIATKLVRPYRDEWWYRHLCACGAVVNIWMMMLANLFGFCLGKDGMVALLQDMLTTFDGLVFFVCSSACLFIASQVMFELRESERRRGIDVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL15</accession>
    <name>A0A1E3NL15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SART-1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_126964</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017371.1">
        <property type="nucleotide sequence ID" value="XM_019159962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL15"/>
    <dbReference type="STRING" id="763406.A0A1E3NL15"/>
    <dbReference type="GeneID" id="30176649"/>
    <dbReference type="OrthoDB" id="5583at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000481">
        <property type="term" value="P:maturation of 5S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005011">
        <property type="entry name" value="SNU66/SART1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152">
        <property type="entry name" value="SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152:SF5">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03343">
        <property type="entry name" value="SART-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="228"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="56849" checksum="2B147D8DD57F7726" modified="2017-01-18" version="1">MKLSLSVEETNRLRTKVGLKLIPVDEKPQSQENNDAEIVRISVEETNRLRNQLGLRPIPTGKTEDVEVENFAKLERQRRKAQEVDKVRADLTERKNELMVRQRMQKGGILNRIDKVTPAGKSAVLDFDSWLEEVGKEIKNEKVKKLSFKKKEKPVKKNLDKDEKEGIVISHDKTTFTEKAGSENGVVLTLKDINVLDDQEDYESFENVQLKREEILKRAFSEKKGAGKLVSLDGDTDGSSSSFSLADLESGKAEEPDSKKRKLVSLTIESSDDENKEHHSGISYDEDGVEAKKISKFMKRDVSKFKRPKKKAAVGQERTRVFETELVEKSFDPVRLDNDDEDEAENELEQALSATRRNMLKNKRLYTAPVEDVKQEENREVIDEGIEFIDNFRAQEPLEKEDVPNSYQRLDDFTKEDSPVENAAGSRYKAILEAEAEKNYHTYGVSNVLNALKSGNRSSQSKEKTGAVEIVYTDDSGKVLNTKEAFKYLSHKFHGSQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL19</accession>
    <name>A0A1E3NL19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_72845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017923.1">
        <property type="nucleotide sequence ID" value="XM_019164308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL19"/>
    <dbReference type="STRING" id="763406.A0A1E3NL19"/>
    <dbReference type="GeneID" id="30180995"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="292"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="562"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="592" mass="66144" checksum="4BDEA04E3B207A5D" modified="2017-01-18" version="1">MDSEKNSSGIPLYTRKNRKGLSSYAWLGFVLVALVASATVLQQCYSPDRAIAGRLGKAGAAAAQPKCPSVGIHRPETYDTYKKTLDKIMNDEGFRNESVSKMQHAVRIDTSSFDDLPLDVGSNLPLFEHFDVFLDSLRKDFPLFHEHTELHKVNHHGLVYIWQGSDESLKPLLLMAHQDVVPISEDSLAKWEHGPFSGDYDGKYLYGRGSGDCKNLLISHFEAVEELIKAGFKPKRTVVFSYGFDEEILGPRNKNAEFIEELYGAKSMYAVMDEGGVSMMEISGTTMAVVGTGEKGYLDVSLSIQKKGGHSSVPQDHTAIGMMGSLIVEIENDKFPTYFVEKNPTFYQYVCLAEHAVDIDPLLKQDILNSQIDEQANANVREFMNRDRFTSYAIKSTQALDVIHGGVKYNALPEFVELVVNSRVAVEESVTFAFKKFLKDAETIARQYNIGLSAQFPYSNGTEVVILPATDVGIMTVKPITILEPSPVTAANDMHWKIFAGTARHVYEELAYPDRFNNSDEQVVVTPGLGTGNTDTKLYWNLTDHIYRYRPGVLPSVNGNSHGINEYIEFDSHLQCIAFIFEYIQSVDEIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL61</accession>
    <name>A0A1E3NL61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pre-mRNA-splicing factor isy1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_20618</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017939.1">
        <property type="nucleotide sequence ID" value="XM_019162165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL61"/>
    <dbReference type="STRING" id="763406.A0A1E3NL61"/>
    <dbReference type="GeneID" id="30178852"/>
    <dbReference type="OrthoDB" id="1739576at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="112" mass="12772" checksum="BBD79D3C736F2BA2" modified="2017-01-18" version="1" fragment="single">RPRATQQCTDVASATRFRAQTLREIARDLTRIHDPLLTEYQVRDINDTLNRLLRERRAWEYRIKQLGGPDYTSGTTTTAGGSGDTDTVTVKGYKYFGRARELPDVVQLLQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL67</accession>
    <name>A0A1E3NL67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate dehydrogenase cytochrome b560 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72386</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017421.1">
        <property type="nucleotide sequence ID" value="XM_019164185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL67"/>
    <dbReference type="STRING" id="763406.A0A1E3NL67"/>
    <dbReference type="GeneID" id="30180872"/>
    <dbReference type="OrthoDB" id="588261at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03499">
        <property type="entry name" value="SQR_TypeC_SdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978:SF5">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01000">
        <property type="entry name" value="SDH_CYT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="18143" checksum="678146EEDEB9A754" modified="2017-01-18" version="1">MFSAANLTRLTRASMASSARGGIIRPSLHMRNIITVRATPVEEESILVAQRKNRPVSPHLEIYEKQLTAVLSALHRITGVGLAAGFLVVTSSYAVGLVDTASLVTFFAGLPVAAKVAFKAVAGVPFAFHAWNGVRHMVWDSGHEANIKGVYKTGYAVLGLTAVSALALLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLH1</accession>
    <name>A0A1E3NLH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_72489</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017531.1">
        <property type="nucleotide sequence ID" value="XM_019164212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLH1"/>
    <dbReference type="GeneID" id="30180899"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF50">
        <property type="entry name" value="HEXOSE TRANSPORTER HXT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="88"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="559" mass="61310" checksum="FAD4381090CEF9A6" modified="2017-01-18" version="1">MESLVQIPPEPLDGLLRTQRREQAAEQDSEQQVNEKYGLERDAEGSNEAVCVDKSSSEHCKGDTEGQELANFNEEEDYVSSYLPITCYSILIALGGLVFGYDIGTIGGLVDLPALSARFGDSVQTGPATFSPIVKGALVSVSCLGGFVSGGVAAKLIPVIGMRCTIFVSMLLYALGNTVILLAPSWEVILVARVFNGVCIGLTTITCPMYISEITPIRERGLFTSLNQLFTTVGIVIGALSILFSATRYYPDDISQYQYPLLQGILLSAIGGVAIWLVPESPDWLVKRSKPISKVKKSLGKLRSLPFDDECIINATAKLFDYNFQMNNIHHLDSKNSTSILKGRPKYFQRTLTGVVLLAFQQFTGINYFFFYGITIFENVELKTPYLVPVIFGVVNLIFSGLSVYVISLFDRKVLLIFGSVSLTLLMAAFTAVGTFCEVNFHVTILLIVLSCLFIAVFSMTWGPIAAVVISELYPPRIKVKAMSVCGSSSWIFSFTIALVIPTLTSKIGFALGGVFALFTFLSIFFVHYLVPETKNVSSTRLDRSYETNPSRFGVVLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLI6</accession>
    <name>A0A1E3NLI6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018112.1">
        <property type="nucleotide sequence ID" value="XM_019164360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLI6"/>
    <dbReference type="GeneID" id="30181047"/>
    <dbReference type="OrthoDB" id="3063476at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053203">
        <property type="entry name" value="Cisplatin_resist-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022024">
        <property type="entry name" value="DUF3602"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693:SF1">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12223">
        <property type="entry name" value="DUF3602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="182" mass="19681" checksum="8E780101A65D9849" modified="2017-01-18" version="1">MSLHTVVSTGRGGAGNMISSQGVNAANEVIMTEEEWKKYQQNGGGGGVGTKDYKGRRGMQEQPTISEEKTYNHDRQGRIIASSGRGGAGNIAKYDKLPSPKIQPESQEIELSPVFSTGRGGAGNIHRTKSNTKTKINLESEIEDQLSPLHSNRSAKSQGSASYRDSSGGDSKFMKKLKKFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLP2</accession>
    <name>A0A1E3NLP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_31695</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018153.1">
        <property type="nucleotide sequence ID" value="XM_019162712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLP2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLP2"/>
    <dbReference type="GeneID" id="30179399"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="6">
        <location>
            <begin position="46"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="231" mass="26364" checksum="16C554D7516C300D" modified="2017-01-18" version="1">MLAAGQPLRLRHPPHLHSVSPGCTWHVPTNTLLTETSPLRFFYRANLRLQKRLAASVLGCGQRKIWLDPAETTELAQANSRATIRKLYKNGSIVKKPTTVHSRAHARALAESKRGGRHMGYGKRKGTANARMPTQVLWMRRLRVLRRLLAKYRAAGKIDRHLYHTLYKSAKGNAFKHKRALVEHVIQAKAEALREKALQEEAEARRTRNKAARERRSARLAEKRDALLNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLV9</accession>
    <name>A0A1E3NLV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol-phosphate mannosyltransferase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_31359</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Stabilizer subunit of the dolichol-phosphate mannose (DPM) synthase complex; tethers catalytic subunit to the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018237.1">
        <property type="nucleotide sequence ID" value="XM_019162696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLV9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLV9"/>
    <dbReference type="GeneID" id="30179383"/>
    <dbReference type="OrthoDB" id="2014333at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013174">
        <property type="entry name" value="DPM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433:SF0">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08285">
        <property type="entry name" value="DPM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="92" mass="10396" checksum="D586DE85E2B53909" modified="2017-01-18" version="1">MTKAYETTVYALAISAIYFALWVGVFPTPAVFQNEVLPVVPWWALVSFGCYTLFSLGYGVYTLQDKKDKYVELTEQIKEAKTFLKAKGVDVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM49</accession>
    <name>A0A1E3NM49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16307</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in ribosomal function.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017536.1">
        <property type="nucleotide sequence ID" value="XM_019161158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM49"/>
    <dbReference type="STRING" id="763406.A0A1E3NM49"/>
    <dbReference type="GeneID" id="30177845"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000740">
        <property type="entry name" value="Putative GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="25"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="252"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="36870" checksum="48158C5FCF485C0B" modified="2017-01-18" version="1">MLLEPEGGSGGGKGQGFEVQKSGDARVCLIGYPSVGKSSFLSKVTKTKSEAAAYEFTTLTSVPGVLEYEGAEIQVVDLPGIIRGASEGRGRGRQVVATAKTADLIIIILDATKSHQQREHLERELEAIGIRLNKERPNMSIKIKKTGGIKLNCLTPPKYIDEKLVTSILKDYKVHNADVMVRDENITVDDLIDVINEQHVVYTPCLYVYNKVDSVSLEEVDRIAREPNTVVMSCELDLGIQDVVEEVWYRLDLLRLYTKRRGTAPDFREPMVVRHDSSIEDVCNSIHRDFKFQFKYALVWGSSSKFGNAPQKCGLNHKVHDQDVVSIFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM77</accession>
    <name>A0A1E3NM77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_40418</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018347.1">
        <property type="nucleotide sequence ID" value="XM_019163109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM77"/>
    <dbReference type="STRING" id="763406.A0A1E3NM77"/>
    <dbReference type="GeneID" id="30179796"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040020">
        <property type="term" value="P:regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="25"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="403" mass="46510" checksum="D7FE3E6FEC89F2C8" modified="2017-01-18" version="1" fragment="single">MASIEIQQYGTDLVQIPVKSLSSRYHQISNLGSGSFGMVALYKVKNGILDSLVEEMMNFPGTLLSPSSINYKYSNDLVAIKTMNKKLKKLTDYSKVKEIQFIFSVDTHFNLVQIIDVFIDRTYLKLNIVMENMDQNLYQLMKMRKGNVFSPRTLKSILSQLLSAILHIHKHLFFHRDVKPENILVMQNLNFFGSRQNIPSNQRNNSYIIKLADYGLARHSRNDKPFTAYVSTRWYRSPEILLRQGYYSFPVDIWAFGCVAVECATFCPLFPGANELDQCCKIMEFLGNPNKSFQLSALQNSNYSYNSYKTNGNNNSYNYGPMVPFGGFWDDAKDLALKLGLVFPKNFGYRLENVIIRKDFSLHEKKDFFQMVKSCLTWDPNKRATAFSLLHSPYFEDTMSVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMA9</accession>
    <name>A0A1E3NMA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Scaffold protein Nfu/NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_31763</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018382.1">
        <property type="nucleotide sequence ID" value="XM_019162718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMA9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMA9"/>
    <dbReference type="GeneID" id="30179405"/>
    <dbReference type="OrthoDB" id="565552at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="3">
        <location>
            <begin position="26"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="264" mass="29719" checksum="3ED3AF0FD572BF9C" modified="2017-01-18" version="1">MRNTVVKSGLRQKQWQGAVVRRLLSLQTMKTPSENALKFVATDKTRFLPEGMQQAIDIESLAEANEKSPLASQLYKINGVRSVMIGSDFITVNKVDNSLSNNPELDWTSLKPKVEQYIASFAKTKQPFLDQHYLEEYQKELDTLDENDDDVVYEIKELINTKIRPALQDDGGDIHFRSFDESTGTVFIKLRGACKSCSLSEDTLKGGIESMLKHYVPEVEEVRAVLDPEEEIAIAEFEKLEKKLKQNASASGPSDPKVRPEAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMR1</accession>
    <name>A0A1E3NMR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein kinase SCH9</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_33782</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017786.1">
        <property type="nucleotide sequence ID" value="XM_019162844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMR1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMR1"/>
    <dbReference type="GeneID" id="30179531"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904828">
        <property type="term" value="P:positive regulation of hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045945">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901494">
        <property type="term" value="P:regulation of cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047484">
        <property type="term" value="P:regulation of response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090153">
        <property type="term" value="P:regulation of sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000116">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="109"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="280"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="542"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="636"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="643"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ46673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="676" mass="76013" checksum="78482074C5F0F82C" modified="2017-01-18" version="1">MSDFAKNLFGFVKPKSTSPETMAPAQDVNEAPLARVVSSSGFPSEILPHATPTIGLNEKSYAVGTTINDPNALNRSINQFDHAQQSNLTAQLNTTQSNQHYQPLLSKTQDYSLASAADAADTIQRGKLRINIMEARGLNVTTNYSQPYVVCTFESSEIVTQGPESYDPKNAQFVHRNNNDYSTRTKSSANYNPSNPVWHHTAIFDVIGSKSQLQISVYDTSIDGSFLGHLNISPKTTDDSSVERWYELQPRLPNEPVTGKIKIKLDYSNINKRHYGPNDFEILRLLGKGTFGQVFQVKKSDSKRVYAMKVLSKQVIVKKKEIAHTIGERNILVRTATTESPFIVGLKFSFQTPTDLYLVTDFMSGGELFWHLQKEGRFSEERAKFYIAELVLALEHLHDNDIVYRDLKPENILLDANGHIALCDFGLSKANLTGNGTTNTFCGTTEYLAPEVLLDESGYTKMVDFWSLGVLIFEMCCGWSPFYAENTQQMYKNIAFGKVRFPKEVLSHEGRSFVKGLLNRNPKHRLGALNDARELRAHPFFHDVNWDNLKEKKIPPPFVPHIANELDTSNFDPEFTNASTSAINRQLMMGTTPLSQAVQENFKGFTYVDDSTMFDHFNGSMGRRGSYKPQYGSFIPGNPNLPPDEEVVDEQSEIEEENEGEDMDVDDEFVSGQFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMV2</accession>
    <name>A0A1E3NMV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15404</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018567.1">
        <property type="nucleotide sequence ID" value="XM_019160811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMV2"/>
    <dbReference type="GeneID" id="30177498"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015916">
        <property type="term" value="P:fatty-acyl-CoA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042758">
        <property type="term" value="P:long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="509"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="818" mass="93170" checksum="E5365471E795296E" modified="2017-01-18" version="1">MYDSSTQAYLNKGNAYLIRSVRNVLLFYSKRRKYILRVCYCLIIYMIFNNGSKSKARPKSNPKTFPESRSRSLVEPVGGGQISQNAQEKVSPQESPSAKKYKPLIAGKAILKKLLYKFKNSSTVALFSQLNLRSPKTLHSFYQLALQVILLFFKAFLTLQIATLDGNLVSSLISRRFKKFFKFLLLWLVIGIPTSITDSLLEKSKKLLSQTIRVNLTTKMLDDYLPSNGNATLYQLLNSKSSAITDPNHRITSTVENFSNSFSILPSQLLTPILDILIAANHMAKSSENASEGALLLGLVANVSTLVLKFFTPNFSTMNNMRNTLENKFHEFHSNIIVNNEEIALAKGHSREIDLLDTSYFEFEKFERMTLRRMAVYNFAISFIFKYTLGAFGLMLCAVPTFTSVYFAGFKPSEELVSGLSSDFVANRSLLLKASDSLGKLIQSKKNIQNMTGYANELWEFEKELIDINESSQLENAINTRKDSHGNNHVNADSQPLLVGPNVSYGDEISFHEIPLVTPNGNVLVENLTFSIKPGDNLLIIGPNGCGKSSLFRILGGLWEVKDPGKLVVPYDKKDLFYLPQRSYFTYGTLREQIIYPDSYDDYRDKILRAKQENPSSPVHDDEYLIELLREVNLDYLLDYDSDLEEEDDIEKDLSRASTSSCVFMIPCLDRVEKWPDILSVGEQQRLAMIRLYYHCPKFAVLDECTSSISSDLERECYRIAIEDLKITVISVCHRTTLWEFHSKILKFKRPEGEGMATTLFTSFDPELRLQRHEELISIDNSLKRSDELTKRLQALKKMKNSRSGRRPALMYIEEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN61</accession>
    <name>A0A1E3NN61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_10534</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018663.1">
        <property type="nucleotide sequence ID" value="XM_019159682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN61"/>
    <dbReference type="STRING" id="763406.A0A1E3NN61"/>
    <dbReference type="GeneID" id="30176369"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="116"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="201" mass="22880" checksum="11316259B0165D78" modified="2017-01-18" version="1">MESVVPYFNKTIDTLAGLLAPYVDDKQLDKACLWLKENDTTVIVSTFGALTLATVYFLSGGKSDQDGRRRGKSYNGRRSYKVKVKKEKVVPVDPIKQAYDIIADVKQELQNAYIPQVDKLEQDVKAEKNPGSTKTATEKKPTYKDSTEYRQLYLNESLLKQLMRLDGIEPNGVEDIRSQRKATIKEVQVQCRRLDALKELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN79</accession>
    <name>A0A1E3NN79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15473</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018654.1">
        <property type="nucleotide sequence ID" value="XM_019160838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN79"/>
    <dbReference type="STRING" id="763406.A0A1E3NN79"/>
    <dbReference type="GeneID" id="30177525"/>
    <dbReference type="OrthoDB" id="10254455at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140570">
        <property type="term" value="P:extraction of mislocalized protein from mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19520">
        <property type="entry name" value="RecA-like_ATAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000538">
        <property type="entry name" value="ATPase family AAA domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF3">
        <property type="entry name" value="FI08533P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="123"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="357" mass="39886" checksum="FA9C6CA6182087C8" modified="2017-01-18" version="1">MNKSEAFKLISECVLLSTASFSVYYLLSTFLGDPSHRTTDERDRTSKHLASLRNRSPELKKVLSSLSNYEKILLAYLVTPDDINVSFTDIGGLDEVVNDLRESVILPLVYPELFTQYGSLLAAPKGVLLYGPPGCGKTMLAKALAKESRSNFISIRMSTIMDKWFGESNKLVDALFSLAKKLQPCIIFIDEIDSFLRERSSTDHEVTATLKAEFMTLWDGLVSSGRVLVLGATNRPGDIDSAFLRRMPKRFSISLPNAEQRTKILDKLLNDVDVECDVQELVDITEGMSGSDLKELCRNAAVNSTREYIRRHLIDVNGDLVGGGGRKITMRALELKDFIDSMEPDRVMKLKLTTDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN84</accession>
    <name>A0A1E3NN84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018683.1">
        <property type="nucleotide sequence ID" value="XM_019160853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN84"/>
    <dbReference type="STRING" id="763406.A0A1E3NN84"/>
    <dbReference type="GeneID" id="30177540"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051698">
        <property type="term" value="F:saccharopine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF24">
        <property type="entry name" value="HYPOTHETICAL FRUCTOSYL AMINE:OXYGEN OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="439" mass="49157" checksum="10B40F33F3B73EAD" modified="2017-01-18" version="1">MDPEEHVLIIGAGTFGLSTALELLRGGHKNVTLLDAYPVPSPLAAGNDVNKIFQSVVRSQFYSDLSKESLRKWRDDPVYQPAYHETGIIYGASMEESYAEIYKQYEMLQEDKDDDSVLLETPADFLRLVGQSGVESLSLDNTQPRFDKWKGYYQKKNCGWTYASLALERASMECIRLGAKMVTDSAQELLFDKKTQACLGVRTYSGLEILAAKTVICAGANSVKLLDFHRQLLAKCWTVGHIRLTDAEIGQLKGSPVVLNIDKGFVFEPDAFGDLKFCNEFPGYINMETIKGAEGEVSVPVYKDAIPKEAETQMRDFLREVLPRLSDREFNVAKICWCTDTPDRHFLIDEHPQHTGLILGTGDSGQGFKYMPIVGEYIASLTLHGRDSLPEDKKDAWRWRPETAVHRDNGALQNRYGGSNAVLDLKDVAEWSRAVLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNA2</accession>
    <name>A0A1E3NNA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15501</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018684.1">
        <property type="nucleotide sequence ID" value="XM_019160854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNA2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNA2"/>
    <dbReference type="GeneID" id="30177541"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="694" mass="79591" checksum="0094CA3488F2EE82" modified="2017-01-18" version="1">MRMFRHVLAESHPSCKHCTRLDLQCSYDLVYRWDEEALQNGTTFGRSNQYKKSYLQNKYADFISEKRSSATAANTFDYFLKSETSSLIKKLSKQAIPWADIKNNVYFVNTTQRDFDKDKFDGTLGKSCQNIASSPLMHVMKTMLMDDFFLSSLEQSEEGEDCSVFTNAGAVDLSREMDEMDFLAFLEQISLRDMLKPGMVSDELAPLAESSALPHINIKYSLTNLNLQSNYHYNRLTSFSLEEQKLISYFVDVVCPTCVCYSHIKKSAVNINPIYLTDAIIPARQWESNPYLYLIVPLAFRHEIVMDAVMATSASQLSSAGNKSFEGLSRFYTEKAIRQLPDLIREKQCLHASNWDDILATVLMLCFNEISSTTDSISVWMMYLSCAKYFVTQVNTLDASSPLANFFARYFITHEVIGQTALMENKDETSKDYSANEIAVQVSSSITDTKGKDEFMKFIFRQGIEGDLYLKTLKDKDTTINVVFGCCPYLICLTHQISQFADCYEGLALETAETRHEFESDISSRREQIVFEIQNLNQEVQITENDDDESEINIKCIAEIKKLSTLIYLFIRIDLEGVYQENGVVTKQFLINRREMEKAKSKILELLDLLPELSVTLLWPIFILGVVATNYEKERWFVLDKLNKIQTIRESTSLKAAQETILAVWKEVDLGLRVLRWKDLVNEKRESLSLALIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNA9</accession>
    <name>A0A1E3NNA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_31370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018698.1">
        <property type="nucleotide sequence ID" value="XM_019162698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNA9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNA9"/>
    <dbReference type="GeneID" id="30179385"/>
    <dbReference type="OrthoDB" id="497541at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="367" mass="42926" checksum="F851576AC7A003C3" modified="2017-01-18" version="1">MKSYTPVPVKVANNKNRKLGTWTPEVFTYPPVEEFKGWSVKDTTPLPYRAFKHTYFFTMGIRSMDWNSWIELDNEWMKYHNNKVERIADRGHELIGTDPVVWDAAIELLTEFRNFLPTRYPTLFKKTEKGINNLVTGEVFEFVNVPRNEFKKDPMEMAALMVQDDLAILKEDENGQYILKGGAIMLAGFWRLRDKLNLPLSAIHTTGDVPKYNEKLKRPMEKFFSRLTCDKPVVRNNYFLQTDADLAWSSSIGPEDKEVVGWNTAEVATDINKMYYRSERQSVRRLPISGCIVFTIRTYFLPITKMCKEPYIPKRLLDGILSWDEDVKRYRGYEKFHGLLLPYLEEQAKIQEAEGYTVDTEPDSYPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNE7</accession>
    <name>A0A1E3NNE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71673</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018737.1">
        <property type="nucleotide sequence ID" value="XM_019163977.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NNE7"/>
    <dbReference type="GeneID" id="30180664"/>
    <dbReference type="OrthoDB" id="20828at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000254">
        <property type="entry name" value="CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035971">
        <property type="entry name" value="CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019035">
        <property type="entry name" value="Mediator_Med12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00734">
        <property type="entry name" value="CBM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09497">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00236">
        <property type="entry name" value="fCBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01281">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57180">
        <property type="entry name" value="Cellulose-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51164">
        <property type="entry name" value="CBM1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CBM1" evidence="10">
        <location>
            <begin position="1773"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1424"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1643"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1443"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1647"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1662"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1672"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1687"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1699"/>
            <end position="1712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1935" mass="216151" checksum="EF37F643D743B838" modified="2017-01-18" version="1">MSEYLSSYDGQMHDPTGVYPLNWDSKPQQSTKVNIPNISNHEEKNIYPDFKVWIQTEEEDKIMKTHLQKGFMEPHFVKHEDQSGRQLITELLTRSNAQSQLTPLERDSKLDLMGQIFVDAMHKRRLFNGIKSKGAYKPPPRVTLTEHKKEAWLKKLANPNIPLQELSRAIPHGLRNKILLEQCLNHQVPIPRAIWLIKCISTNEQRQLKRKTTNVSAMTINANKWIVEWTEQITSFFESIIEGCFNPTLAKEVWRFRLNYTIELVVNLYSEELMNQITFLTWIIRYASQIINNAHVFADIKPISVHQMIIKLFWFKIIRYDYLTKELSETMLLTLVKANQLPRPSKFDSLALKITGIFQYLIKYLFYYNSDIFILPSNWNYLKPYLRKVLDMHLPPVSDQFKLIAYRNESLTIDELDRSGPNDDDTGSPVNLIPNDKFSLILHRLNCTEKESISSLSKLVFEDSSLFDSTTWKDYMPLIFQWCIQAVGEREVNFHRINMALSILQFRQSQLIQSKSKKFKQFKADLENKIIDFVYSMSEVLNYRNEENSKGSIYDVNNFLVLISRLYSIKLFVVSSYLRRLIASGVIYLSDPDRTCYIHILILNLFSSLNDSNLKSILKRLMDSTKITIDNHDLTSIKESLSTFSQVVFSTKTDRENSLGQYKCKEPFPLDDPTQINKYIQLSEYFYQQLEKQLKESQQKIILNRSRLLALYECFQIYPIGLSRFLTLLLDKLNQESNDFEFDNMETFVVFLKIVFVNMKLLKFSIFSSKQSLWEHCLGVLSSWMDSNRYCITENLHNAKLPTFVLSQFGAKAAKIEAVKVDPVFLTVEELLSLDLASYERLSNAAEFFHHTTLAITRYANAVRGAEESVNIALIIKFMRSLYSWKQEEFTKCLRDYLFRYLKPTLQLEYELNSRVLLKLVIDDFISIKQIVEIFHIKATSYTFFENDFDDVHLLWDVFFNTRIEFDFGEWFLYEFSKFSYISDNRRNYYKILSEIIYTTFQKAPMDEPMQDGVVNVSNDVEVDSVDVEVGVHVGVDVNPTVGVDVMDTFHHLNDVRKINSSQHRRLFQQNIINNKLLSTFWELISSHTDLCIEYYYVPADEYDVIDVRNLQKFVFQRLMNFHTNKEFDNIQVIENLNYLNLPVLRWTFTYMMRQKYKESVQSHEATTQFITLVECILGIVGKGSIDCKLVGELFAFLPDSFKHHLLNACEEIYLNSENFPSVLMDGVNVTQYLNCIISSCSRLQLACNSSSASSSASGSKCLELSDALVFSLNLALEKLLTICHNLDHQQKKRLANRTGLSRELELGVKMVSKIILLHKYFLVELILKRSVNLQRDVLIMNATKLFNHKIMLKNPKLKNLLYDVLISLKVIISESITQQFQRSQSAGTSSPGVWINRGAMGATAATPTNASFFSPINSPKPASASLAASHAVPTTTSPLTHDASNANTAADTASGHDLGDTKPHGLGHGLLHAPSHGHGHGPGSSRTGKQGSAPKRGVAAFGNKATNALSNGNSAAAYIIMPNVLNIKPPSFNNNLRSLLSMFDLRDTVPEKTIRLYTAAQGWDGAVAADRSVAKYTPKPFELIEDSKHPKKHIQRKMSATTRGNNSMIALCFAFLLSQVVHASSDSNPYVQNNGSLDGYYEQDGSSSSSAETALSESTTKWWTPESNTWSASAAAASEPASSESTTKWWTPESNTWSAPAAASEPASSESTTKWWTPESNTWTATALASTAAASAPVASAAASAPVASAAASTPVASAAASAPAASAASGKCQAEWAQCGGADFTGASCCQEGLTCVSANSYWARCVSSTTLASGVASQPASAAAPDTAAGTGTLTAPAGSAAADATTGVTAPAAISAVSAADNSNNSKPTAAVTGVSAAAVGQSTSIAGFAKAVNSISKQASASSSNGAAPSSANGGIAAAIVLGLGLVLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNG8</accession>
    <name>A0A1E3NNG8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018757.1">
        <property type="nucleotide sequence ID" value="XM_019163982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNG8"/>
    <dbReference type="GeneID" id="30180669"/>
    <dbReference type="OrthoDB" id="3996108at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="160" mass="17600" checksum="B88EE45493891E03" modified="2017-01-18" version="1">MTIIDHSSIKRVSQDDGLESITLRSDLAARYTFLNETLLEGIGAVAVDNRFRTMLGLKEVVQDDAGQLITDETAYLRRWEQRLQTEVRQLEQTAANEPDAPHKTADTKDTLDTLLLDLDRPFPASSTTAPVDEDAVLAAFDADCAHVTAVLAARTDNSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNL8</accession>
    <name>A0A1E3NNL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15606</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018798.1">
        <property type="nucleotide sequence ID" value="XM_019160887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNL8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNL8"/>
    <dbReference type="GeneID" id="30177574"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000359">
        <property type="entry name" value="Small GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22546" checksum="5D5AF27481B1B99B" modified="2017-01-18" version="1">MNNDYDYLFKLLLIGDSGVGKSCLLLRFAENTYTQDYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVNQWLQEIDRYATSSVQKLLVGNKCDLTDKKVVDYAVAKEFADSRGIQLLETSAADSTNVEQAFILMSKQIKDQMANAQPAANASNKPNVNLAGTAVNSQQGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNT4</accession>
    <name>A0A1E3NNT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ferric oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_31819</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018316.1">
        <property type="nucleotide sequence ID" value="XM_019162725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNT4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNT4"/>
    <dbReference type="GeneID" id="30179412"/>
    <dbReference type="OrthoDB" id="10006946at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF178">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00463">
        <property type="entry name" value="AIM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="11">
        <location>
            <begin position="133"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric reductase NAD binding" evidence="12">
        <location>
            <begin position="416"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ47203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="760" mass="87377" checksum="B7AF47829BA3EE32" modified="2017-01-18" version="1">MYDITTTIFTSTTSSVSSISTASTTSRFQLLPQHPSSDPSWKTTRRKVTLFYGYLTFVITILFIIILPCWNRISNYLYYKFLQISRWLRLNPKVAFLHVNPMSAQMIIFWSVILSVLSLCQSNMDLRFIAARLGRVPVYCLPTVLFLTLRPSPLSDVLYLQLLPIHKWLSRIVILQSLLHTLSYIYLMSKSGSLDKLNRFDNKNGVYAMLAFMVILITSLPFFRRSYYKFFFINHYICTWIATITLYLHVRPGIPYLTALNCFILIYQIYYKFKMTKITTVSIMKISDLMVVADFPNDALSAKHNLPGCHIRLIDFDESHSKLWNYLKMIIVPIQHPYTVASLAVDKSQKLIIRIGNYKLKDHHKYFITGAYLPHLSFIQKLSTANSSMSNPFSMIHNPNTNNMNSLLVSTKVKKCLIVVGGSAISFALPILRVLNYNGSMVKIIWVIRDHEDLKVLDYFKNYLINDDCIDIFITGKYTMYEKENFKEALSELHKKKQELELLQETEILSGGYSYYNINEDRINSESDHQKSPVEDAVSFSSSNKSMDLKSNFDSEMETSPLIDHNSKKPSYGASTLHPEFDRFHRKSYIDFKNHNQKISTSYNDETIDIELHEADRKDNARNLSLNTMIPTSFTSPVNHKILPGSASISTASALYDDLEDYWVLKSSYSHIEFGRPKLGLHYYSWCIGSSCIGPLVSLQSGQSICYNIREDPKARFKDRNGKPDDSIWIIGAGPSGLVDNVRLWANDCGFSFHEESFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNV5</accession>
    <name>A0A1E3NNV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL27m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">54S ribosomal protein L2, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15674</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018889.1">
        <property type="nucleotide sequence ID" value="XM_019160918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNV5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNV5"/>
    <dbReference type="GeneID" id="30177605"/>
    <dbReference type="OrthoDB" id="1867012at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000042">
        <property type="entry name" value="50S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_bL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_bL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041244">
        <property type="entry name" value="Ribosomal_bL27m_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL27M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18471">
        <property type="entry name" value="Ribosomal_L27_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL27m C-terminal" evidence="9">
        <location>
            <begin position="137"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="372" mass="42940" checksum="F52472C9B8F26B60" modified="2017-01-18" version="1">MLSAFTDGLRSTVVRTALQFKQQVRYAHKKVVSSKTHMQDSPGKRLGPKKYEGQEVKVGQILFRQRGTKWYPGTNVGIGTDHTLFALEPGFVRYYLDPFHPKRKFIGIALKKDDRLPYAHFDPTPRRLGRVVLTDNYATKEAEYAPRKVKLLQPKIEEALKERENARAAKKESYVKQLPQFKSLKDLNERELSLASERLLKIDGFLRGGKTLEDARYYATYNYKYDTKLELNRKDLTADETEAKINEYTQLAEKVDNAVMFDARLQLTDNLTTEQINDLKVEAIAKLDSLIPDVTKPISKKTKEEALTLLSKPCFSLKEQIGLKRKYLKPTLPESEETVGTAKDKKLVVIQKMNIETRRVETIYRKKNYFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNY9</accession>
    <name>A0A1E3NNY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15699</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018918.1">
        <property type="nucleotide sequence ID" value="XM_019160924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNY9"/>
    <dbReference type="GeneID" id="30177611"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="469"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="609" mass="70113" checksum="FB97D8AD1CD28DEB" modified="2017-01-18" version="1">MAEEYGLRKRNDSLNTTWPSLSLNIDIQTSHSKSKDTNDGKGKVRDRHGNSGKQERDDDGSKKLKRFTRGSVRGKQIGTTLWNVLLSELNIEPGLEKENIENRDQSGNTNTDIDRWSDNLELLLNYIRLPMHLERFMTFGLLYSAVIFLKWLVIIPLRCFIHTVLNIVRFVQRRGSFSSPFVKHHLIYKNDLLSVTGIMTTLFLLRNLDTSRIYHNIRAGTAVKLYFMTQVLDIADRLLSASGQDILRVTYQFNSITVGNRLSLVKVSQFLALYVLSILYLWFHSYALVYQVMALNVAINSYSNALLTLILSNQFSELKSAVFKRTEREGLFQATCADLNERFLILIMLAIISSRNFLQILINTTSINDFFDSLKPDSWSTQLTQWKSFDNWLGLLMGPSILVIGSEILVDWVKYAYIIRFNRIRPKVYDKFTKILANDFVNGFTSDNFSRINHQPNEHPSLLTKRTGFPISTVSIIFCKLTLFPYLQYLISVVYERNNNAHIASCLIISTVLIALVSVLISLRLLLSLIILSWSRVLLQRQLPKQTDYVSGDPNVNLSHVADVREHLYDDTEALPPSLEDLRLQKLHKNRDDKLTGVVRFEMADKRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP13</accession>
    <name>A0A1E3NP13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15710</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018935.1">
        <property type="nucleotide sequence ID" value="XM_019160927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP13"/>
    <dbReference type="STRING" id="763406.A0A1E3NP13"/>
    <dbReference type="GeneID" id="30177614"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF27">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="123"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1077" mass="123904" checksum="53EDB193C8C9CA8C" modified="2017-01-18" version="1">MVKDGSSSIENSAEQKNGESETNTLQSSSNTDSELIKSANDGSYTQGEESSTMQLTEDNGKVTQTIGNTNERLEDQNESESQKHEEVISVTSVDEDIRYSSNIPYQLIDILNTTPFKFSDSPESRITCIEAWELNLYVGTNLGEIIHLYKIDDALGYMQISKQTFSSSSTSKPINKILLLPEVSIALIHSGTTVSGYLLPELSPANIGKAKDVSDLSVDWKDLKLDKNKQNRVTLKEDYYGDPYVKVTLFTKRSIKLLRIFNDSIRLHKELQYLDVVSGLQVSNFSIVSNSINYDLVDISQSQKIYLFPISTSSAKTELDPIIRYVKRNELLLICGGTKCSDPSMGMFINLNGDVVRGTLAFDSYPTSVEIDYPYVLVILQNNTLVVYSIHDQQKLQEIEFSDSSSTCIKIHESSRIFELKDSELAKKMTLAPIISTMDNDEIERIAVESDNAVKKSVSLSSCIIYDTTGKYVKILKPLSKFDRWMKIYESCDNSDCLGLYDKLSQEYQESGSNRFLITLLGLFALKFQLYDQAFEVWTSYFKHLDPRLMIYIFNSGSDGIYGSVWTYQILFDSIRELQEAKRSNDMKDFFKLYLNTCLAMEFKENAIDIGKSVEVALVNLGVSNNEDLEPVIHEIKYSTNEIIEILLLNKKYFLLSKFYSKLKDHRQLLYYWKGLIDGELEDDEFNKNFKDEKKSLQYLVNYILTNCIEDSVVIDKYSEWLLKAYPKFGLKLVTDKRVKQLQLNDIKILNLLTCEDEEDQKNLKLTYLEYIFENKGEKHFLGDLILIYLDMIISIYERNLKVKNVIDKAITEYLELDTPKISIYKYWKLTKDAELKSEPFTIYHDRLYSYLNSVSIGTQSILEQQLVLNKCKDRIIGTKYSQIFPLLAVMILYRFEEYENVIDEMIRLKDYSTAESFAVSIKLSPLDSSAKIESIKMQGSIDKNNSSSVLVNDETMTTVKNDHSKLTENLLKKIFDIYLQMNDTKLIDGFLNRYDLLSDTTEKTTSTLDRMDKFVEVINKVPENFPLDKLTRFVTNNLIEFRDYNDHVHIKKTLVKMEVNRMTKLSRNLEGENSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPA5</accession>
    <name>A0A1E3NPA5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71598</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018651.1">
        <property type="nucleotide sequence ID" value="XM_019163961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPA5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPA5"/>
    <dbReference type="GeneID" id="30180648"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="105"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="364" mass="40905" checksum="C48BCE4876A66886" modified="2017-01-18" version="1">MDSKHRSTSTTTITLLPQLRKLARGAQFYWYLALVTTIWYTGLCMFSSILRGTSHPTTIRHYSSALLAILVTYLIILRQTYKNKPPAYVIGQIWHLMEHLLGSRGSSRSSKEQASHRTAAHPDPGSPKTAAGAGTGGASGSSNPGSEVNTKPSSATTAAATASTTAKTNILRDENVQYLLFAFFHWLFASPFFGPINPSTLYPFAIYAIFHASHYSQTSIIPYLPYVATTTKQRWCATIVHFHRLYNERSRMLASNTEVLLVTFYLGPFFKLFFRIALGRFWSAAAGGSAQFWYDFKTLVLCLVTIVFLRARFAVDAYTRTQIQSYDALINRFVWHPMLPQQLRQLLMGLRKVVGDFVEMVNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPD0</accession>
    <name>A0A1E3NPD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="4 7">2.1.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="4 7"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019083.1">
        <property type="nucleotide sequence ID" value="XM_019164061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPD0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPD0"/>
    <dbReference type="GeneID" id="30180748"/>
    <dbReference type="OrthoDB" id="425211at2759"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000003">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="306" mass="33619" checksum="01710A7D69998FEE" modified="2017-01-18" version="1">MSLGRQLASASRRYSSYPASKTIRQNSLQDLYKLHKNKEPITVITAHDYITGRFAEASPGIDAILVGDSLSMVAKGHPSTLELDFDDFYHSTASVLRAVREKFVIVDMPFGSFEESVEQCTRSAVKLMKLGKVGSVKIEGSYEMTDRIRSLTNAGIPVTGHIGLQPQKFNMLGGFKAQGRNALGALDIYKQALHLQKIGCKMLLFECIPEKVAAFITDRVDVPTIGIGAGNSTSGQVLVMADALGMSDGKKAKFVKSYLDINSLAVDALTRYSADVKSKSFPNSELHTYPMKDEEFDEFERMAKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPD2</accession>
    <name>A0A1E3NPD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_58283</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019045.1">
        <property type="nucleotide sequence ID" value="XM_019163289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPD2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPD2"/>
    <dbReference type="GeneID" id="30179976"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903450">
        <property type="term" value="P:regulation of G1 to G0 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.690:FF:000001">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000002">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041093">
        <property type="entry name" value="Dis3l2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17877">
        <property type="entry name" value="Dis3l2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="3">
        <location>
            <begin position="525"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1087" mass="122346" checksum="7921C2C193AC1E67" modified="2017-01-18" version="1">MSLQPPAPTNRSAHHRRSSSNSPYQQQEQETSPRNHRRSQSSITSVGSIPFSKTDSRPQALGHTRRHSLGLAEAKKAAAEAHAQRYHNSPSKLSSFSDRSPQRSFQFPPKDPSQTLQPPQPAFVERHQPSGLGHRSTGSDLSSNWRKQQPATPTHNNNSNSVSNFESPAFLPGHRSRNSYGGSISSISQFNQGQRKSLFAPYLPQNSLPDLINEGKLVTGILRVNKKNRSDAYVSTDGLLDADIFICGSKDRNRALEGDLVAIELLVVDEVWSSKREKEEKKRRKDTKFNSDLNDDVHNDASTSSNINEQSNSSYPASNSGSLNRKNSLKQRPIQKKNDDVEVEGQSLLLVEEEEINDDAKPLYAGHVVAVIDRIPGQLFSGTLGLLRPSQAAKKEKEKDNDSDKKSSNHDNDNRPKPKIVWFKPIDKKVPLIAIPTEQAPKDFVENHDKYANQTFIASIKRWPITSLHPFGTLVARLGATDDAAVEVESILRDNNFMCDEYIDGQEKYMMDFPNTDYILSNSNRKQFNDDYIIACSQNGQFCDHAFHVKGIDGNKIELGVHILDVSEYVLNDTNLDKRAKKRSHGVFLPQKSVNLFPDNINKLISFNEASNSPALSVVFEIDSLTFEIENVWMGETEIKPSKRVNYEEIDKILTDLEDSNHKNDSAISSYFASMALIASRLKRKRLYNPTLNTKNMLPLLDQLDDERVKLSLNIYDNIESTFIIDEIFHNVNAAVAQKIHSALGGRAFLRRFPIPTLNKFEYYQSKIAQLGVELNTTNAGSFQNSILSIDEPLKREAVETILVKCMGRGKYVIAGKTDQELSHYLLNLPTYTHFNAPLRRYADIIVHRQLKHVINNTVDNYTDDVDSLKVISDYCNFKKDCANATQEQAIHLLLCQTINDMSSETGQVLCSGIVLQVYESAFDVYLPEFGIEKRVHGDQLPLSKAEFDKVNKVLKLFWDPNVDSSTFIPADENEPLSYRASIKNKFRIPALDVAKSQDDSSKSNCGLLSDEMYEKLNNLKLKPPHGENPLAPYLKNLNVEVEGTNHIQEIKLLDKVPVILRSEIGMSLPCLTVRVLNPFCETQGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPK3</accession>
    <name>A0A1E3NPK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MATE efflux family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_30723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019075.1">
        <property type="nucleotide sequence ID" value="XM_019162651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPK3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPK3"/>
    <dbReference type="GeneID" id="30179338"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="58628" checksum="D6BDDB8BBFA826EF" modified="2017-01-18" version="1">MQETVSLLQGVPPEPYHAVTQAEARVLYESTCSVRKTNTNGSCWPGADSDAEADDTEFFPLHPVTTTTAHELVYLLKNSVPVFFTFFIQYLIQILIPTYFASQQGAVHMSSCTLSITAFYLTGPVLVNGFASSLDTLCSTAFGAKHYYRVGRYYVQCTLLLLVLMLPSMWFWSHSFPFFDRITHISYPDDSQLPDLCASFLSVFTFVAPAVVIFECTKRFLQSQCRFSVPTRVVVCGIPVAIAINLLLRSYLTDERYQHVAPAIAFVLTYWLMAISLLACALLLDQYHCLPSLDELKTWTLHSFLTDLGIFFKLGVPGILMILSEAFAFQILTFSAITFPKSQLAAQSIVATLASLAFQPPYAIGICCSTHIANIIGARSSNYKPAMSAIYIIMALMSVFNFTWFYFLREQIASLFTSDKEILSLASKLAQIIAVNQFLDCFNVICAAVLRGQGRQKIGSVMSLIAYYIVGLPLELYWGFKLNFQIFGLWIGLALAVNFLSIVELIIVYKSDWITIMKQNHKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPL7</accession>
    <name>A0A1E3NPL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pseudouridine synthase RsuA/RluA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019118.1">
        <property type="nucleotide sequence ID" value="XM_019164071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPL7"/>
    <dbReference type="STRING" id="763406.A0A1E3NPL7"/>
    <dbReference type="GeneID" id="30180758"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="1">
        <location>
            <begin position="136"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="410" mass="47050" checksum="5B651E48D385EDF5" modified="2017-01-18" version="1">MFGTLFTQAFSTYTLPVIVHNVSPINSPLPYFRLHIIRSNPSVKRSLLDILNSKPYRFGFTKDQLKHLLQKDLVQVWVSSVRVKITIPPHDNDFFDRIMKAPLSPYSVITYNQHIHETPVPTPERLKILSSTNSSLLAINKPPGIPVHGVQKYFYNTIQSMLAEQLACKQVTLYPLHRLDRLTSGVLLWATNPTTVSKFKNRNAWCRKKIYLARVKGRLPNFSTTCTDDLVYLYPTRHMVRVFQGSITSFKEVCYDEKRDESVICAFLNTGFPHQIRIHLRNLGCPIIDDPLYSKTGKYKEITRSRNDVTDAYWDEVIQRSKEIQSKKRSVDSASCSICNAPKYHVPKTNEHGDYAICLHAWRYEYKGVPGGPYAGERHSYETPIPSWAIPIGTNEEFVKGIIVYHTTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPZ3</accession>
    <name>A0A1E3NPZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="7">eIF3i</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 39 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF-3 39 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF34</name>
        <name evidence="10" type="ORF">PICMEDRAFT_15944</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit I family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019219.1">
        <property type="nucleotide sequence ID" value="XM_019161030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPZ3"/>
    <dbReference type="GeneID" id="30177717"/>
    <dbReference type="OrthoDB" id="24966at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000127">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="11"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="53"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="289"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="359" mass="39976" checksum="85D8806526945AD6" modified="2017-01-18" version="1">MSNKLMRPILLKGHERPLTQVKFNREGDLIFSSAKDYQVCLWYSANGERLGTYDGHKGSVWSVDIDSTSNYLITASADFSGKLWKAETGECIYTWNFESPVKRVEYSPDCKKVFFITDHVMGKQGKIQIFDIDFTKPEQESKPILEIINEGDATTKPTTATWSYAGKHIIAGHADGSISKYDSTTGEQLEYSTPFKKTITDIQTSSDRTYFVASSKDQTAKLFDIDSWKLLKEYESESPVNAACITPVKDYIILGGGQDARDVTTTGAGEGKFDAKIFHKIFMDVIGRVKGHFGPLNCIAIHPEGTSYVSGGEDGFIRLHHLPKSYFEFQYEVEKTAEALQHQNASSSSSGNEQIEASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ05</accession>
    <name>A0A1E3NQ05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_72158</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for protein translocation in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019293.1">
        <property type="nucleotide sequence ID" value="XM_019164114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ05"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ05"/>
    <dbReference type="GeneID" id="30180801"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006398">
        <property type="entry name" value="Sec61_beta_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="84" mass="8870" checksum="21514F44BA0F8DC5" modified="2017-01-18" version="1">MVQASTAVPGGAKTLAKRKATQENKLKQQQQNQPNSSRAAGAGGSSSSMLKIFNEEAQGFKIDPLVVLVFAVAFIFSVLTGNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ07</accession>
    <name>A0A1E3NQ07_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_123735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019245.1">
        <property type="nucleotide sequence ID" value="XM_019159919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ07"/>
    <dbReference type="GeneID" id="30176606"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="149" mass="16739" checksum="660CD8E2D19E211B" modified="2017-01-18" version="1">MYPFSECKTIPPIYPLQTSISASGSCPATRHPYRSFSAAPRSVGRARSALARLVADLLQRCWAGAFPLWGTMPPDLYARQKHYCCRPRCSRNYKRAGSLLVGSLRGICCCCARSFFLLGRCSWFLFFSFSSLSSRCVVFCFLPLFSSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ25</accession>
    <name>A0A1E3NQ25_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_23039</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019320.1">
        <property type="nucleotide sequence ID" value="XM_019162292.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NQ25"/>
    <dbReference type="GeneID" id="30178979"/>
    <dbReference type="OrthoDB" id="294853at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="5">
        <location>
            <begin position="2"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="3">
        <location>
            <begin position="221"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="4">
        <location>
            <begin position="941"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1622" mass="184961" checksum="B22EB6F6E89EB490" modified="2017-01-18" version="1" fragment="single">IIADLAALGVDAKRKNPEIRHACDHSIELLKPFSLSSLNPDNVMGPSGNDSPALVDTLRVHPEFIVPLLMACHSKNTKLIGDAVKCLSKIIQLKLLPSIESTPEGSPEPIDGVVDALMEASNAGPEIQVKILQLLPSFFQLYAFRVNAETLSKMLYICTTLQAASKGLVIINTAQATFSQLIDIAFEKVTISDTTPDKEVCFTMYEVPIDNDKCIQVKQSVYDAQRLVSDLCTLIEHHKPSFLKTNYITEDYGFEILESLIKNNSEIFLEHVELSFLLRTRVAPILLRFVSSSKDFTLMVKVSRLIFLLLNDEFDVLRVESEVTLTLLTHILSKNSGAPQWKKTMVLEIYVGVFKNHELLKKIFMAYDNNPTEERKSVVDDLLKVCLNIVHDQKNVLNTGDLIQLPATTNEPIQNGKKQKPQQLSQRKVVYPPGLRAAEFTKTIRFMDSIEKQEPPQTPPSYDSYLISQILIALSDCVQSSTLSLMKAANPAPYISEQLFAESKDEKLKVLYECICDLITNTWALQLEITDIFIHSTLDNELFSGTLKLLENLCYCSGVLSLNKVKQSILKYLSVCTLKLDGRSGYQSRVMTIGESIVGTISSTLGQAVSNMSNNGNNDVPSSTSVQMYPRTINTRQTLCFHTLIRLGVSLGSHLHDDWEIILIVLQWVSYYINGTSGMSKKDVPPLSPYLMNRDLQIIEHSLSELSKSIFNQDETTFSYILKSLISLSEKVMTPEPEREFGQSPLTTSGEIQPCIFNKLFYVNKLTDICVINPIEFMIYPENNFDIINDYFCKIADDRSNSDETRLLASRSFNQIAKASAETGFEDKSIEIHELTETKVLNNMCKFMTRLSQLPMSNELLIANCEAEMYLQTLETLKNIIDRFGSLIQLTWGVVTEMLDFPFLIISNCDSDILKEKIINDIIVSVLKSSFETMKVVLDEILQCIPKNQIKVIIDSLYHFVEQNFDLNISFNSVSYFWLISDYIKDKLETNSNNSEFNYSLASEAQLIEYVSNDSTDDYQYYQYLWIYLVLQLAKTSPDKRVQVRNGAILTTFSVIQSFLTDASQYSVLYDIVIRPVILQIKPPESTIAFSSQDQKDWMESFINITNGLTKLLFSFMNSSFSLESPQIITMWQGIMEYFVGLLNLDFNWVELNNQIFKNFYEILHGFKECDKDLPKDVLESLFEPWASVKINYNLAKEAVYQTSLCSFVDCFPLSMHLFKPIMTTSKFEKMLMILNSCIRYPILVDSRNDNMKCTALQQAVLDNLSLLEFDKSDSSYISYESLLIQQLNLIVVLPFHTRDLIVKKLGDKGIKIPTFVAASYQGLQILERHLAKISDLTYFNDRSILKVIKSLLEPSKLKSDIYFTAKSSEGEEEKEYLWVIGFRILVNITLKVLNLIMNTPEMNFRTEVNIDSLTQYQKMRDILLKFFNMYYDTNKACKIEKSQIEQFISSVWYASFYHNQDIIIESILPPVSHQLSALTMDKLAKELIRPQNWDIYGTTRMLNVSPRLRLSIQCFDDLVLFVNPDANIRIWRTCLPFFISRCTLVFRKYLMDIKLLGGRPVSRILIVELRDAVNGLQTVLSCFQDLNTADSTQVISKLKSVYSMLIELLPKVSEKDIEAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQF0</accession>
    <name>A0A1E3NQF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_72265</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019408.1">
        <property type="nucleotide sequence ID" value="XM_019164136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQF0"/>
    <dbReference type="GeneID" id="30180823"/>
    <dbReference type="OrthoDB" id="1053771at2759"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019280">
        <property type="term" value="P:L-methionine biosynthetic process from L-homoserine via O-acetyl-L-homoserine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03312">
        <property type="entry name" value="CIMS_N_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000003">
        <property type="entry name" value="5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00172">
        <property type="entry name" value="Meth_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006276">
        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003556">
        <property type="entry name" value="PRK05222.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE N-terminal" evidence="18">
        <location>
            <begin position="4"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="17">
        <location>
            <begin position="440"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="394"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="394"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="706"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="445"/>
            <end position="447"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="445"/>
            <end position="447"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="656"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="658"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="738"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ48295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="766" mass="86169" checksum="B48EC8D58ADBFC9F" modified="2017-01-18" version="1">MVSSAVLGFPRIGAFRELKKNTEAYWSGKIDAEQLLKVGKDIREKNWKLQKEAGVDIIPSNDFSFYDQVLDLSILFNVIPDRYTKYELSSLDTFFAMARGLQRKATETTKAVDVPALEMVKWFDANYHYVRPTFSKSTNFKLNGQKPVDEYLEAKALGIETRPVLVGPVSYLYLGKADKDSLDLEPISLLEKLLPLYVDILKKLADAGAKVVQIDEPTLVLDLPENVKAAFQTAYSKLAAAEGLPELILTTYFGDVRPNLDAIKELPVSGFHFDFVRTPEQFDDVVSIIGEKTLSVGIVDGRNIWKNNFAESVDFVKKAIAKVGADKIIVATSSSLLHTPVDLTNEKKLDAEIKNWFSFATQKVDEVVTITKAIAGEDVSAKLEENAAAIKSRKESKITNNPEVRKRTSEAQADESMAKRHSDFPTRLVSQKAKYNLPLFPTTTIGSFPQTKEIRINRNKFSKGEISAEEYEKFINSEMETVVKFQEEIGLDVLVHGEPERNDMVQYFGEKLNGFAFTTNGWVQSYGSRYVRPPIIVGDVDRPAAMTVKESVYAQSLTKKPMKGMLTGPVTILRWSFPRDDVTQKVQSYQLSLALRDEVNDLEKAGITVIQVDEPAIREGLPLRAGSERSDYLQWAAESFRIATSGVEDSTQIHSHFCYSDLDPNHIKALDADVVSIEFSKKDDPNYIQEFSNYPNHIGLGLFDIHSPRIPSKEEFIARIGEILKVYPADKFWVNPDCGLKTRGWEETRASLTNMVDAAKYYRGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQM1</accession>
    <name>A0A1E3NQM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_81611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019513.1">
        <property type="nucleotide sequence ID" value="XM_019164990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQM1"/>
    <dbReference type="GeneID" id="30181677"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133478" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10863" checksum="F56800E55317CC14" modified="2017-01-18" version="1">MSTGIKACLMLSVLWRSGTAGGRKIQQCSKNKAKLVRNDELCFECSGAGSCVDVLCGRRLCLQRRLICRGSRRELTIFRTQEAWMIVRRCQLPRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQR0</accession>
    <name>A0A1E3NQR0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_81414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019495.1">
        <property type="nucleotide sequence ID" value="XM_019164988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQR0"/>
    <dbReference type="GeneID" id="30181675"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="83" mass="8873" checksum="D5D750575A71123E" modified="2017-01-18" version="1">MAWPAEAGDPRGPLLYCRRVVATPTLPGREARAAARLLRERLSPRTEGPVGRHGLNGARGKGGRRKNHVCVGEKCNAFCGVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQT2</accession>
    <name>A0A1E3NQT2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019567.1">
        <property type="nucleotide sequence ID" value="XM_019160191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQT2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQT2"/>
    <dbReference type="GeneID" id="30176878"/>
    <dbReference type="OrthoDB" id="4072889at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133469" evidence="2">
        <location>
            <begin position="27"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="251" mass="26885" checksum="B04D21B62370B813" modified="2017-01-18" version="1">MAFFAKLKCYLTSALVIWALYHVATTCPLCKPLPYGVEQEDSLCKYTHTGYSYVKPYTQPIVAPVLEYYNGSPVKPYVDESVVVVQENYNKFVGPYVPVIQAQAEAAYAVVLDKLAALKARLDDDTLANVKKIVIPDGDNIDAFIGTPNPEQEKTVDPVTPQEDLVEPTPLPAASETNVVPNTEAAEETASSGSEPTEEVEPIPAKTDGTAAAEPSSNGKLEKLIEEAESVVGQIAAEEVIEAAQAAGQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQZ1</accession>
    <name>A0A1E3NQZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_70115</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019595.1">
        <property type="nucleotide sequence ID" value="XM_019163559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NQZ1"/>
    <dbReference type="GeneID" id="30180246"/>
    <dbReference type="OrthoDB" id="10036721at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17756">
        <property type="entry name" value="MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000241">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="326"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="726" mass="82049" checksum="901C859DD845F59A" modified="2017-01-18" version="1">MSFERADTYATSVLPPQQDASSDQNSEIIKAFKRFILEFRVDNDFVYRNQLRENILSKQFQLTVHSENLISFHDELHQRLFENPKDIIPLFERAITEIAKRNIYLTDEEAPSVFPQCQLILLSNDNKISIRDIDSDNVSKIIKISGIIISTTMLNSRASELTLMCRNCRHTLKIKSNINLGLPQYPKKCLAPPLPDGDKPNCPPNPYLIVHDKSTFIDQQTLKLQETSDMVPVGEMPRHITLFVDRYLCNVLVPGTRCEIIGIYDTYQKKVRNTGSVNNAAIRSPYLKVLGVQTDADLLRSAGGLNRIFSEEEEEEFIALSRTPDLYERFVKSIAPSIYGNEDIKRAITCLLFSGSKKVLPDGMRLRGDINVLLLGDPGTAKSQLLKFVEKVAPISIYTSGKGSSAAGLTASVQRDPATRDFYLEGGAMVLADGGVICIDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIFGRYDDMKAPGENIDFQTTILSRFDMIFIVKDEYDAHRDMSIAHHVMNVHTNGGNNEEQTEGEIPIDLMKRYIQYCKLKCAPRLTPQASEMLSSNFVAIRKEVKLKESLSSERSSIPITIRQLEAIIRITESLAKMELSPIADVRHVEEAMRLFNASTMDAIKEGNTDNDEVLQHIENIESEIKRRLPMGWSTSYNTLRQTFVEKGTYSIEALNKTLYILERREVVQFRHNRSSIFRCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR03</accession>
    <name>A0A1E3NR03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_27144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019607.1">
        <property type="nucleotide sequence ID" value="XM_019162481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR03"/>
    <dbReference type="STRING" id="763406.A0A1E3NR03"/>
    <dbReference type="GeneID" id="30179168"/>
    <dbReference type="OrthoDB" id="10252926at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="238" mass="26699" checksum="9A873F4D9C84197F" modified="2017-01-18" version="1" fragment="single">TMFQWMFGKRLTPQEQLRKNQRQLERTQRELDRERVKLQQQEKKLVAEIKKSAKQGQTKTMNIQARDLIRTRKQITKFEKMKMQMQAISLRLQTIRSSNEMTNSMASATRILSGLNAGQLNLSQLSKISNDFQRSMDMMDQKQEMVDDVMDDLMDDEIDDEDEEEIDQVVGQVLDELGVGLNDKLGDLSVSKTSPGAVAAAEAHAPARAAVALGDAGSAGVGDEDELQARLNSLKKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRR8</accession>
    <name>A0A1E3NRR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">coproporphyrinogen oxidase</fullName>
            <ecNumber evidence="4">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14329</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019908.1">
        <property type="nucleotide sequence ID" value="XM_019160327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRR8"/>
    <dbReference type="GeneID" id="30177014"/>
    <dbReference type="OrthoDB" id="15318at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1500.10:FF:000002">
        <property type="entry name" value="oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="entry name" value="Coproporphyrinogen III oxidase, aerobic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003727">
        <property type="entry name" value="PRK05330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="COPROPORPHYRINOGEN III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755:SF0">
        <property type="entry name" value="OXYGEN-DEPENDENT COPROPORPHYRINOGEN-III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="330" mass="37291" checksum="8BD311E365749A09" modified="2017-01-18" version="1">MSDAKTTTTTKAGESVLDASLPIKEQMERLIRQKQAEICAGLETLETKQFAPDEWVRGNNGGGGRSMVLADGSTFEKAGVNVSVVHGTLPPGAIERMKNDHKDIETSAAGTVGFFACGLSMVLHPWNPHAPTMHLNYRYFEIINPDGTTQTWWFGGGADLTPSYLYAEDARHFHLQHKQALDPFGADLYPEFKAWCDRYFYIPHRGETRGVGGIFYDDFNRLPAQRCLEMAESCFNAMLPSYLPLVARRKDTPYTPEEKQWQQIRRGRYVEFNLVYDRGTKFGLQTPGSRVESILMSMPKTATWLYNYEPDSGSREADLLQVTRSPVEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRS8</accession>
    <name>A0A1E3NRS8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019903.1">
        <property type="nucleotide sequence ID" value="XM_019160326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRS8"/>
    <dbReference type="GeneID" id="30177013"/>
    <dbReference type="OrthoDB" id="3994831at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021624">
        <property type="entry name" value="Saw1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11561">
        <property type="entry name" value="Saw1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="245" mass="28466" checksum="C097E6943D485F1C" modified="2017-01-18" version="1">MAPVRSHINFTASHVLPVRTFLNRQQIQKNRVKHSLLMCPQSGDSKSENHEDGRRLPVQLSKCKRMVISNRHLRELYGDPQIKKKLAAILIEMTKEEVFSVCSNTNKRGVKGGAFDDDDTQKNDSLNQHTFKDSETLIIPFTNDSWKCQLIICWDDIEFIRDWMCGVERHLGRTNWNKCGKLMLRKISFAEADTSRNKRKEIIELIDSDIEESELSVRDKKNINFEYEKTLLSPITLNVHVHSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRT7</accession>
    <name>A0A1E3NRT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019386.1">
        <property type="nucleotide sequence ID" value="XM_019161080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRT7"/>
    <dbReference type="GeneID" id="30177767"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="56" mass="6225" checksum="0B1F574D337BE752" modified="2017-01-18" version="1">MPLFDQIAEEKAVCKLSWISYFGGLAMRIGDSIELNQPILRRQADVGKVGFASKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS41</accession>
    <name>A0A1E3NS41_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_83350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019641.1">
        <property type="nucleotide sequence ID" value="XM_019164997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS41"/>
    <dbReference type="GeneID" id="30181684"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="150" mass="16195" checksum="40A4EAD633AEDB3B" modified="2017-01-18" version="1">MHVPSLFELPKHEHITHAGWHLGDVNYVDSLHVCLSACISASRASGSSRLQPARLGAAVVCYIVAAAWACDLLASPTIDQLPSPARTLVPSLLSPLVSRPALLCFFPAHTKTDLCVCPSAIGRFPATHRSPAVNVYNRLQAYIHTPIKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS80</accession>
    <name>A0A1E3NS80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_75573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020064.1">
        <property type="nucleotide sequence ID" value="XM_019164938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS80"/>
    <dbReference type="STRING" id="763406.A0A1E3NS80"/>
    <dbReference type="GeneID" id="30181625"/>
    <dbReference type="OrthoDB" id="3998161at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="478" mass="52524" checksum="1EEEED7C3A2A519A" modified="2017-01-18" version="1">MGLLLDQDIGSNNKNIKVDRVSHDEISQHTQFGLRSSKETISKRENDIIDAGDIQDINQTIKADVVLNAEYNNEAPSTTMVAVVYDDSISENLEKYPTADSKDEKGHKICQSCRLKKKKPSSTLGSGTTSNEGSIVRNVGVEQLFTKTKHLRTSRSQISSKVLLKPNSTTIMEVKENGENLTSPEKSRSSNNKEAILLLGKKRQIEKVTGDISSDDLVSANFSKSEDKSFGYLPNSGRERHNIVSKNCKKSYGASSADSNQRNTSKNEAHDITAMKDLSETQTTNVKGHVVKKRKVALNKLNETGSFLNVTGECDKSESSASQQMTIIQEDLEAYRGHNIINEKPLHENMMLSQSFSIPHDHSQLNRQLKQLSQYTSHSSTSGGDIEDDDDESVLVNLDGLEEIGMGYDLNDVEAETSTVRGENVEYEEEKETMLRHVRAVQAGLLSSTGEPSDAEIAAAVEAVAVAAAVARSRGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSS7</accession>
    <name>A0A1E3NSS7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_97250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020320.1">
        <property type="nucleotide sequence ID" value="XM_019165126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSS7"/>
    <dbReference type="GeneID" id="30181813"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="210" mass="22197" checksum="A520820597BD36EC" modified="2017-01-18" version="1">MPERQSARAPERQNAKALDRPFPTSLGSLPAAVRAAVCSVYVGPHFRTSRPSPGCPGNRAASRWRSCTACAVLLTCGARGRRAGVARLRDTPFLWKDVEGGGPGGAANISAPVLVPPHGDVLFCCCCCCCCCCCCCCFAVLLMVAGGEANSTHEAIKSPVWESSILTEPSARRRCGPEADRESGHAQHTAPGRRHVGRPARRRQTSQAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT43</accession>
    <name>A0A1E3NT43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin 3 binding protein But2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_9708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020343.1">
        <property type="nucleotide sequence ID" value="XM_019165123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT43"/>
    <dbReference type="STRING" id="763406.A0A1E3NT43"/>
    <dbReference type="GeneID" id="30181810"/>
    <dbReference type="OrthoDB" id="18530at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40368">
        <property type="entry name" value="YALI0F14399P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40368:SF1">
        <property type="entry name" value="YALI0F14399P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133490" description="Ubiquitin 3 binding protein But2 C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25006" checksum="F3E63118FA31668B" modified="2017-01-18" version="1">MLRTIILCLLSCTAVLGSKYQPYPISDLDPTTYKPYTKGQLVPLQCIRRQIDNGEHVFDSEGNIVFAPFMNCLETNSPLSLKYMEDSNVKCTVKFEDEIFHLFQLYLHKDAPFTCRYELRPNSGIYLPIDLSFRGNVLESHFDIDPNLNVVMLSNNENEIVAGSAFSSSTNTTKVIIGQNVELSFNIKWTNAHKEIIGDDNVGVYWIASRNPWLVVALVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT68</accession>
    <name>A0A1E3NT68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_14712</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020357.1">
        <property type="nucleotide sequence ID" value="XM_019160493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT68"/>
    <dbReference type="STRING" id="763406.A0A1E3NT68"/>
    <dbReference type="GeneID" id="30177180"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="13">
        <location>
            <begin position="30"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="12">
        <location>
            <begin position="493"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="638" mass="72290" checksum="C8B6686FB9916C95" modified="2017-01-18" version="1">MDSLTENLKKLNLVVPAPVADSFPEANTVDLCRNYITEQLSELAGVDKSIIFPALEWCQVLEKGDLLLPLPRLRLKGNLDEMAKDLAAKFPLGGYIDRVVPQGKFLQFYFNTQFLLKYVTKDVLTRTLDFGSAPLGNGKSVIVEFSSPNIAKPFHAGHLRSTIIGGFLSNLYERLGWKVIRMNYLGDWGKQFGVLAVGFRKYGSEAELEVNPIQHLFDVYVKINNDIKAEQEAAEAKGETLDPNNCIDGEARSFFKKMEDGDKESVALWKKFRNLSIEKYIDTYARLNIKYDVYSGESQVSDEVMKNVSKTLQEKNMITEDRGALLIDFKQLGQKKLGKVLVQKSDGTSLYITRDLGAAIERHEKYNFDKMIYVIASQQDLHTKQFFTALDMMGNKWSKDLVHVNFGMVLGMSTRKGTVVFLDDILEAVKAKMLEIMKQNSEKFAQVDDPEKVADLVGISAVMIQDMQAKRINNYEFGWDRMLSSEGDTGPYLQYAHSRLRSIQRSAAVPDEDLLNANMSIENVCGSLEKLKALNLSEEEFTKKYKLLESQIEKTQALARVMASYPDTLRYASKNYEPSTVVTYLFKLTHQFSSTYKVLRVIGEDREVMIARLALFSAVRQILHNGMILLGITPVERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTE9</accession>
    <name>A0A1E3NTE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="10">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_29144</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 10">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020465.1">
        <property type="nucleotide sequence ID" value="XM_019162575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTE9"/>
    <dbReference type="GeneID" id="30179262"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF28">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="11">
        <location>
            <begin position="18"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="470" mass="51708" checksum="E5D2ED70A7F9C1C6" modified="2017-01-18" version="1">MVHDTLSASHRAMVSGHLKETDPEVAQIIEDEIDRQKHSIDLIASENFTSTAVFDALGTPMCNKYSEGYPGARYYGGNEHIDRIELLCQARALKAFHLDPEKWGVNVQSLSGSPANLQVYQAIMKPHDRLMGLDLPHGGHLSHGYQTDTRKISAVSTYFETMPYRVDLSTGLIDYDMLEKTAVLFRPKVLVAGTSAYCRLIDYKRMREIADKVGAYLVVDMAHISGLIAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFYRKGVRSVNPKTGKEIYYDLENPINFSVFPGHQGGPHNHTISALATALKQAATPEFREYQEQVLKNAKALEVEFKKLGYTLVSNGTDSHMVLVSLKDKGIDGARVEAVCEKINIALNKNSIPGDKSALVPGGVRIGAPAMTTRGASEEDFVKIANYIDATVKYAKKVQSELPIEANKLKDFKKKIAEGSPELASLKTEVYAWAGDFPLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTH2</accession>
    <name>A0A1E3NTH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_101846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020558.1">
        <property type="nucleotide sequence ID" value="XM_019159644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTH2"/>
    <dbReference type="GeneID" id="30176331"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133494" description="Secreted protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9792" checksum="EAFFB1C3BA6B7C14" modified="2017-01-18" version="1">MTVCYVLSEALFALFAPSLLPLSSSVFASSSSPWCSFMLPARAEALRSSPGPPIGRWPSAILWKRVTNRGKKRQKCENQPKRTPGRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTM7</accession>
    <name>A0A1E3NTM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LAA1-like C-terminal TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020459.1">
        <property type="nucleotide sequence ID" value="XM_019160546.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NTM7"/>
    <dbReference type="GeneID" id="30177233"/>
    <dbReference type="OrthoDB" id="192608at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040108">
        <property type="entry name" value="Laa1/Sip1/HEATR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046837">
        <property type="entry name" value="Laa1/Sip1/HEATR5-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057981">
        <property type="entry name" value="TPR_LAA1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663">
        <property type="entry name" value="HYPOTHETICAL HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20210">
        <property type="entry name" value="Laa1_Sip1_HTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25808">
        <property type="entry name" value="TPR_LAA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LAA1-like C-terminal TPR repeats" evidence="2">
        <location>
            <begin position="2132"/>
            <end position="2301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1500"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1500"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1524"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1541"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1554"/>
            <end position="1565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2322" mass="264804" checksum="64D9157224264339" modified="2017-01-18" version="1">MDREELSFETWRPHLVYNNDTDANDLSYEKSQAYLFDKLTRLHLALQPINAAYRPELADDLVFAFDQINALLDYLVHTYEKSKHIKLLSVNINNLIAKTITFDLSVMMKNGHEKFNILLIELVNKLSDLFLANLKKISMSKNWFSSLKHLAVIVLHYVFTKFNGFLNNAKNPLLTALYKHLSKANDSYNSSIESGVFKSNFFSDMIHLVDIILTHDNSNLILDEKLFNRLLKLFKFVTKQNASSSSSLSSSSSSVPSTSVPASSNTSLYTYPLVSILHSFSILVSLLKSDRYISSLSSSKKSAITSTKYMSSIKEHTSVMFKAMDTDHKKLKLCLTKNLSDLLVFTFVVFGANIKDIDFALDSCITFLLAEYRKSSTTSNVKSSLIETLIQFISKLNLYYQTSRVKISRKESHANFVSYKFFDIMNIIYSNLFEFPTLDPSKKRKLTKEEQSEHYILINNTIAMNFAIKTLNHLDLIHKFMIDELDNDINKLIVLSKIILGNSSTEDKIRVQCLSQVVDGDNILNVWYAANLLKFAQILIEDLNEFILSDHGNLSTSNINSDIASQLINKISSLCVNKNFRVRVISVETLVKLLRVKPDMSFGIMNESLKKLTSAFDISGKSVNEEYKGSFNDNYSNSYLIVSILSFSSKDYITSDFVLKVFTLSLEFLKKFNTSVVSTNLLGSSSGVTISNLNYEKQLVSWILLLGLFNYASNDENTKSNNLFLQDPTQFLTIWKNLLAHTIPKGFIQIDQKTKGITNISEIMKIIEIKNQSLVCLIAYISYMNSSGGLQNDDLIRQLNQILIKSYTFISTFTSELSGMNVPQFLDNSLCGNKLRIFEAYLKLLPHLNIKNEINSTLLLDIVKKFCDIEKFRYQFKDPFGKFIKTSKKRIHVNSISEYSIYYIDDGVWYGLTSKFNNFKIDELMIKDKTYKVVNNRSESDIDLVPFHTQFLVESPHIQSNLSNILVFDDTAESRNFNFLNHSLLHDAFMLLYNNSYDVGYTDTMKYPVPTDTMTIDTSIELFAISFPHLSSKIQLSVLESIRSFVFYKMKESRSIESTNFDESEEGSIKLEFSLMLRKKAISINTSIAFHSLLNYMLRFNTNMSKKLSLKKEVANLIIETLKNINFEDIYLVNLNSESIGICCSLIDDSSVQEEMLNNQVSIIINSIAESSNPNVRAFNIKTLSQLTKFSSMINTSSITKTIFTLLLDPHPIVHAASLQSLDTFLSGKSNLEISSNLTHVIIDYLEKIWLNDNFGIRSATTISSNINFREHSNSVNMMVKLVRSLINTAGPTIRLWSDELKSKLSNILFNFQFLVTFDYEIVVRELLKIYEELLVFDKTIINMSNYKMLTKLLIINNFKIGVYGHSLSNIPFDDEYDNENTHECFPCTTSSKLLNISLESAFQLFKLEDEGIIDDDFENLLWIALEIDPGNESIQNIVTILLEDSMKKSSEEKFGWFNKLLNYFNISKAALLSPLMKTFVKRINNGGMYFHIPISETKATSSKKNSNNNNKETVSNKISNNAEDMDAELAEAEETLTSEQPKRSSEFSNSRENEEEDDDDDDDYNGNKLKIGANEDDMINILDLSNEQVNWKFKLFIIELLNKLLSFCKTDQKLKLSLSKKIPEFVRISFISSSSNLITLRISSLKLLGEIIELYSDMSDPLYPDVSILDQQQAQIMSTITPSFNKGSTVELAGEGIVLSSKFISSNITDIRKVGRIIKILTTSLENLAITAKGSPGSNEKENTFTKIGDLLILTKRSENKIKIYVLQAWARMMILNESNDNSNAELNTLVQNYAKVLIPLWVHTIREYAMLKYGTLKDLNFDESNEGLDLGIYEGCWVDLVEAIGIVIDDKNHYSYLVNILGDDIGKLFMVIFSLCLEYITKRSNKMKLNYSPDDMRILKSLRKLMKLELSIKILFNDYIFAEFVDIINKLILVSTESSTVDLLSTIDELLKDIFLLYFNDLRLSQTDENKQEKSPEEIYSDFDKLFELLRLVIKIVTSKLAFIKYKEISHNTCINLDGADLVLIKKCFTSFIEMINKLPPPIQKDLYSNLVYMQVLIHELNDAKLLTILLPVFQKSLANYKNLMNDDDTDDNKNLRNLFKIFKIQDSSKENQLLTFIILKTIPDIHLGESDIEIINTMIVSGLSSKDSNLISLSVQTVKSIIEHIEYTESSSSANSKRILMKLIPLLIDTMSKDDVELTEPRLFIEIFVIFIRKIHNFDSGVAAESTKLDSSYQLVLPILVFFNSRFGYESYIHDKLLELVEIDPAVFKKTIKNSNDSLKSAIQRLVNVNHVEGESESGEIPNNASTSKEAHIELKTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTV4</accession>
    <name>A0A1E3NTV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_95588</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020221.1">
        <property type="nucleotide sequence ID" value="XM_019165101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTV4"/>
    <dbReference type="GeneID" id="30181788"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="58" mass="6769" checksum="95552D4101687628" modified="2017-01-18" version="1">MFFCVCSWGDVFLDETTCQQLKQQPKQKKPFEAGFIYVVIFYSGREKRLVSATIIKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU27</accession>
    <name>A0A1E3NU27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Elongation factor G, mitochondrial</fullName>
            <shortName evidence="8">EF-Gmt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Elongation factor G 1, mitochondrial</fullName>
            <shortName evidence="8">mEF-G 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Elongation factor G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MEF1</name>
        <name evidence="10" type="ORF">PICMEDRAFT_14986</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020663.1">
        <property type="nucleotide sequence ID" value="XM_019160623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU27"/>
    <dbReference type="STRING" id="763406.A0A1E3NU27"/>
    <dbReference type="GeneID" id="30177310"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04091">
        <property type="entry name" value="mtEFG1_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000001">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000022">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000015">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000558">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047872">
        <property type="entry name" value="EFG_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009381">
        <property type="entry name" value="PRK12740.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636:SF2">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="65"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="74"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="146"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="200"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="772" mass="85327" checksum="CF518CEB47B7C237" modified="2017-01-18" version="1">MFGRISHALGLKSLASAGRSSLPQGVLSSKRGFHFSSKLNTYEEEKVIIDELSGQISDTDVARLNNMRNIGISAHIDSGKTTFTERVLFYTGRIKAIHDVRGRDGVGAKMDHMDLEREKGITIQSAATYCSWERSPEKSYHFNLIDTPGHIDFTIEVERALRVLDGAVLVVCAVSGVQSQTVTVDRQMKRYNVPRITFINKMDRMGANPWKAIDQIRAKLKIPAAAVQVPIGSEKDLIGAVDIIHNKSIYYEGAQGEKLRITEEIPEELKDLVAEKRSELIETLADVDDEIAECFIDEVEPTIDQIKGAIRRSTIARKFTPVFMGSALANKGIQPVLDGVCDYLPDPSEILNTALDLANEEKSVNLVAASNSPAVALAFKLEEGKYGQLTYLRVYQGKLRKGSMITHVKTGKKVKLARLVRMHSEEMEDVDEIHAGEICATFGIDCASGDTFTDGSVDYSMSSMFVPDAVVSLSILPKTKDAANFSKAVNRFQKEDPTFRVHFDKDSKETIISGMGELHLEIYVERMKREYNVECTVGRPRVAYRESILSASAFDYTHKKQSGGAGQFAKVMGKMEATSLADPEDIGATLGDDVKFRNHFQTKVVGGKISEKFLLACQKGFEDCCEKGPLTGSRVLGVHMIVDDGATHVVDSSEMAFRTATQFAFKQAFMQSQPVVLEPIMNVVITAPNEFQGAVIGLVNKLGGLINDTENQADDFNLISDCSLNQLFGFASKLRAVTQGKGEFTMEFKEYQPAPPQLQKQLIEDHQKKNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDB7</accession>
    <name>A0A1E3NDB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74952</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015228.1">
        <property type="nucleotide sequence ID" value="XM_019164925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDB7"/>
    <dbReference type="GeneID" id="30181612"/>
    <dbReference type="OrthoDB" id="10535528at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="340" mass="38250" checksum="EB8029C408B88DC1" modified="2017-01-18" version="1">MHCFIETLPVEIVVLILQQLCNKDVVSFLTAFTCFVERNPLLKEYACHKVTLHLSLTRSGLGVLAKDGLELVSPQDWGLFRSMNVGLERAKVQCYIGLDQIGRLERLQRVRVVHHDGELLSKLLEVLPVGVVVLEVVLDGAVKGKWKKDAAAAVHDDWRFGKKERALKMVTVVNTSKWNQKTVKGISTRMLKRDVLGPAHITDTAILRSSYGTYDMGPNQLHHLGLSLGKFLYESRGSVQNIGVFGIDAIWLMDKTGWEFPELRLVKVGGESMARTVIWAPNLLQKNQEKKEFASGQFVRPMVVVFSKMLNQQCKVVLNGNRYVLVGGSGVELFREYTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDC4</accession>
    <name>A0A1E3NDC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_60780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015238.1">
        <property type="nucleotide sequence ID" value="XM_019163460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDC4"/>
    <dbReference type="GeneID" id="30180147"/>
    <dbReference type="OrthoDB" id="10311079at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="306" mass="34296" checksum="9CB0CA60F1DDF2DE" modified="2017-01-18" version="1">MPIRFPSKVHTMMNQPVPVMMFQSNSPAFPYMSNHSSCSNFKNESEVYGKKATIDNGRYRKINSNEYSCSTYQSSDYRSKPRWENHNTKGLKSSDESLNFISDGNSSTPKKQKIATKSSDTKTLANPLSTVPSGKHENVDTHNVLDGLDNLDMDFSDNTITVASITETQDAQKLKEDIKNEFKNSVQLIAKKTQISSSPEVPNEKLTEEKENCRYGKDLVEKVALNVIGNEKDNEAKISSKVSHFPSCPLPTLSGGWPKAPTVDNQYNVPLYDMNENSYPRFMASPLFGNPIWNNPNAFTNSARDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDE4</accession>
    <name>A0A1E3NDE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015258.1">
        <property type="nucleotide sequence ID" value="XM_019162076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDE4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDE4"/>
    <dbReference type="GeneID" id="30178763"/>
    <dbReference type="OrthoDB" id="277449at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="12854" checksum="903D2BF6A55FFA4B" modified="2017-01-18" version="1">MAQRVTFRRRNPYNTKSNQIKVVKTPGGKLVAQHLKKKATRPKCGDCGVALPGIATLRPRAYASISKTQKTVSRAYGGSRCANCVKERIVRAFLIEEQKVVKKVLKEAAAKDKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDU0</accession>
    <name>A0A1E3NDU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA wybutosine-synthesizing protein 3</fullName>
            <ecNumber evidence="3">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_37070</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="3"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015418.1">
        <property type="nucleotide sequence ID" value="XM_019163054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDU0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDU0"/>
    <dbReference type="GeneID" id="30179741"/>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000003">
        <property type="entry name" value="tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="13">
        <location>
            <begin position="7"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="284" mass="32332" checksum="A24EF34599C3AE02" modified="2017-01-18" version="1" fragment="single">LMDGFDQKKEHLLKEIVFNSESNPDASPKGTIDALCLPLIFLINSHKNMVTTSSCSGRVSVFVEGSKELGIEQKNAEGEKIIKIGAKGDGGRWLFVSHEKGDIQGWWTHKDVALKFEAPPKNGDYSVETRYVLFKYEPLILHVQCRDFKAASRLYSVAMGCGFRESGIGANNNVAIRISLRLDVPIGYINENGNITSAVEESYLQMITKLAYDRFLVNEEKMKKLYDRIEQDIIKQAESGEEKVETKEERKQRKMLEGLSRRDEVRKLKEEKKRLKQLEAENAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE01</accession>
    <name>A0A1E3NE01_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015480.1">
        <property type="nucleotide sequence ID" value="XM_019164820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE01"/>
    <dbReference type="GeneID" id="30181507"/>
    <dbReference type="OrthoDB" id="3997736at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="212" mass="24162" checksum="BE4826A1EAA3800D" modified="2017-01-18" version="1">MKPPALFQDASRQPLPYHVLQQQQQQQQSQSQSQSQSQTTQTTPQQPQDPFVPGRTLSLLQLFSNRATRGATWRLFITLSLTQVVVFFVCSNAILDELEDTEGELSNLWDFYERKDISQTVMAVHIGQLKQQLLSVGAKPVSAQQALTLAQHLLSFARDKDTGNLMIYVDPHSQIYRLVPFSYEYDIKKVPRLDAAVNKSWDEFNRGPKEVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE06</accession>
    <name>A0A1E3NE06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MINDY deubiquitinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015439.1">
        <property type="nucleotide sequence ID" value="XM_019164807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE06"/>
    <dbReference type="STRING" id="763406.A0A1E3NE06"/>
    <dbReference type="GeneID" id="30181494"/>
    <dbReference type="OrthoDB" id="10261212at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="402" mass="44617" checksum="04472DBCC3184BDE" modified="2017-01-18" version="1">MAGQSKSFAITLVSEGRPVLRQNENGPCFVIALVNAVLLGVSDLDTGFRNVLVDMAMATPPKQVSLDTVYAFLVERVLHRAEPDADAYAASTDHASSGQIMDTLPILDSGLLINPAFNNIQVLDFGDYSPSIVRMLDLFGLKLYHGFIMPSELLAQLEGRDIKPTFDQCQDYLVSQLEKEEKENGSDDSADTLAKQIDEFLSNNSTEFTPAGFQVLLDSLNDNQIFLFFRNDHFNTCIKHEGSIYSLVTDIGYINQPDVVWLPLSIYDEGDFLNFDFEISRIKSTPQSVSDVPTDSALQQQEDYDLLLAKQLQLQEDNEISKKLQKTFDKEEELKKQAPLAKNNAITPSKKALNSNTKSTSKKPTQPSRKVESKGKPIGKLKSKSNPKAQSKPSNPQSCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEF9</accession>
    <name>A0A1E3NEF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tag1-like fifth Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015638.1">
        <property type="nucleotide sequence ID" value="XM_019161989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NEF9"/>
    <dbReference type="GeneID" id="30178676"/>
    <dbReference type="OrthoDB" id="5596576at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059066">
        <property type="entry name" value="Ig_Tag1-like_5th"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26153">
        <property type="entry name" value="LEA-2L_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Tag1-like fifth Ig-like" evidence="2">
        <location>
            <begin position="716"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="847" mass="95645" checksum="1E92EBF2F6F31F94" modified="2017-01-18" version="1">MSNNNVDVSRVQFFDKVTGQSNHITHMTNSGQVRDNHKGLDDEYDETVGLLMAQPVPVRYNSIPSLIRESEDIIVDEQPTAFYSRWRLMFYILTGIIMAFLILLIGTLLDLKYVLKSLNIQIGGIEVLETNLSGILVNVQSIDVDFQSAGILNWIEKLNLTEIEFVNDTMVYDQLYQPLFKIDTLDKNLTFTVYEESTHHWIFDLDELFVNIEGDTLSKTVNQLSTNKTSVFHISSRMRVAGKTFSVSKTVNFEKNKLDGFLDRVLSTLVSGVSVQNFQINDYNPAIGFKGKGNAVLKIDSIKNMRIPKIGSSIGFMIEDNLYKVADVNFTNFANIENEHFVVFEFTLFNVEETIIKNGTIDDILWRILNDDSKKEKLQFTILGNGVDDDGWIRDMWEDLVFDIGIRLHDIFGSTSFSCDPTLNDVWNLDVTDFNLGLDNTSGRLTFDAHLLYFHDLLMKNLRANIDGSILTDGINVTFENLTINKMDDLFIGASIQDSVVDIVDVNRSKKMVQNLLDRNAYSQYVSTEIVSRIDLASDFFKGPININGTFNVCLGKFLSLSIHNFFKISDGFIELVDMEYIDSGSGYVKIGVDSNLRLPPFMNTLENEIKKINTAVKYDAEEVSDVTMLTFKSEDGVIPLRLEVALNSGDDDKKRKVEEFVGEMISGVTTNVTLHGVSYENQSSIFGCNEKICELYDGISIPLSLSSKDITGGESEENYFIRDTIMHVISKEVEMTLFNPISNRNLILEIEEGEAICEGYIIGYLKEKVIWEVESGIWTSPRAKVEYANTGSAGWKIIENAIKGDGKINNMTVRAVVKVYISGDTEWSGLNIMYQNTGQTNGKVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEQ7</accession>
    <name>A0A1E3NEQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_36765</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015724.1">
        <property type="nucleotide sequence ID" value="XM_019163036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NEQ7"/>
    <dbReference type="GeneID" id="30179723"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000110">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="237"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="581"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="148"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="462"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="848" mass="93098" checksum="51239DAFF4E233C9" modified="2017-01-18" version="1">MTKQKSKPGVIQRDLDNKVYNHILQLLDDKTDTLKSSALSKDEASNGVFTMALAKDLQFDEIVQYIRLKDKSLGRMKMDILRKSIERTWTEIRDEEAEEISTLGIDLASTGNDDPNFIDGEEDPAAELMEVKDLNLANKNIVNLWKAQESSPANKDNIDSNDPLNVTSNTENNLNGNKKRKVKDKESKTKRVKVDRSPPNMSLKSLGGLDDVFAELMEVVGMPVLHPEIYISTGIQPPRGVLLHGPPGCGKTTIANALAGDLGVSFINISAPSVVSGMSGESEKKVRELFEEARGLAPCIVFIDEIDAITPKRDGGAQREMERRIVAQLLTCMDELTLEKTGGKPVIVIGATNRPDSLDAALRRAGRFDREISINVPSEPARLNILQHMTENLKVSGELGLSELAKLTPGFVGADLKALVTSAGVAAIKRIFSNLSDENGKLVGGLDNDIDGNDNNDQMDIDNDEFVTPLTTPLTPSNANSLASLESHDSDKESSTIQKFLAKHPDPLTAKQLEPLSITREDFLTALPTIQPTAKREGFATVPDVSWANVGALSNIRIELHMAIVQPIKRPEIYLRVGITAPAGVLMWGPPGCGKTLLAKAVANESKANFISVKGPELLNKYVGESERSVRQVFSRARASVPCIIFFDELDALVPRRDSSLSESSSRVVNTLLTELDGLNNRRGIFVIGATNRPDMIDPAMLRPGRLDKTLYIELPDSNERHDILKTLIKSNGTPVSNNVDLCKISHDERCRNFSGADLSSLVREASVIALKRKIFIGIKDLDESVEDHEEIMDVEVTAEDFDKALDTTKPSVSDRDRMKYEKLNKRLGWDKTTVNTSTEPKDDAADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NER2</accession>
    <name>A0A1E3NER2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_55424</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015725.1">
        <property type="nucleotide sequence ID" value="XM_019163182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NER2"/>
    <dbReference type="STRING" id="763406.A0A1E3NER2"/>
    <dbReference type="GeneID" id="30179869"/>
    <dbReference type="OrthoDB" id="190105at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="137"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="284"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="402"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="442"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="484"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="616" mass="68769" checksum="32CE47C863262A09" modified="2017-01-18" version="1">MDFAHPNKKRHIENSLIHSSNAIELSASSQSNLNLTSYLEDHRTSLPLPSPSASPIHSSAENLGGPSDDTNGPSETALLTQSYPTSQADKFSLLTSLVSNLNNASPEVNALLFQLLQKTDKKALSFLHSTLFKVLKKDLITTLPTEIISNILQNLDHTNLLNCFQVSKKWSNVISHFPELWKNLIFKEKLITTEDDYLEDYELIKNNRPDLSVDKIPQLIYQRRLLIQKRWMNTNYKPKRLIIPEESLDIVTCLQFDKDKIAVGSDANHIIICDTESGKLLKTLRGHNGGVWAMKFYKNTLASGATDRSVRIWDIERGVCTHIFRGHTATVRCLEIIEPRQIGTDDEGNPIIYPEAPLLITGARDNALYVWKLPINDGKNDKVSDVPIDLHASNNPNFVTVLRGHTAAVRAVTGYANIVISGSYDSTARVWDLRTGECKWILKGHTGNIYSCVYDVKRNRCYTGCDDNTLRVWDLNTGETIAILEGHQSLVGLITSSDNVLVSAAADSTVRLWDPDTGQTKKVFRGHRSPITCVASDDYKVLSGSQGMLKLWDVQTGAFVRDLANDVDGAVWQVAFDYRRCVAAMQRDNTTFIDVTDFCPAEDDPWAHPSSLATTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEX2</accession>
    <name>A0A1E3NEX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015231.1">
        <property type="nucleotide sequence ID" value="XM_019162064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEX2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEX2"/>
    <dbReference type="GeneID" id="30178751"/>
    <dbReference type="OrthoDB" id="3997504at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="527" mass="60198" checksum="53E2DD4D24FC89F3" modified="2017-01-18" version="1">MSYFTRSKRDASSIAPTMAEEPLKRVKVEKKPKKDDIEKDVKKERMEQERKERKEREMKQKIEKIEKREKKAKREKEKEKERKEKRMKREKRELDKERERREKKERKAKEKEKEKEKSSSYSLFGEDSPLENINNFQLLPSNHEKPYDLNHDLKFQRQLRLIQLSNAHLSSMAHPDFDSPASFEESDLDLEDDLEDEDDPLFDRSFEEMDLTPHFDMDFELDGDLDSDYNVNFTPNERSSSPLSDIDSSFPNDRFNVAIPIIDDVKTGISQVDNIIDQFKFVPNYADTHSGSESSSIDHVSNDKTFEGGNAGTDNNNNNALAMSNNGELVSADAHKDSFANNNEFNENNNTSNNHNQTNTPSLSLNLKLSIPTSAAPTNDSNILLSPSSSFNLLNLSDRAPNSRKQQTPTSFSSSSLFNSSASAASHPPPIVLSEFLNFELPQQQLSASVSSSNNSLTSAPTPAAAKLSFEYRHNNRKINLALTKEPELYRNCRQPYKSALNRLALWSGKAQEMVLDRSLPMDEFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF57</accession>
    <name>A0A1E3NF57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015839.1">
        <property type="nucleotide sequence ID" value="XM_019161884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF57"/>
    <dbReference type="STRING" id="763406.A0A1E3NF57"/>
    <dbReference type="GeneID" id="30178571"/>
    <dbReference type="OrthoDB" id="10050400at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031942">
        <property type="term" value="C:i-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040201">
        <property type="entry name" value="Mrg3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="555" mass="63038" checksum="EDBEC25462528A17" modified="2017-01-18" version="1">MLSRVKRLPVRRFPSRFLISTSRRLLSNAKSDDLSKQPALNQFQAESSKQASKLPEPAMKTPPSTAVPPVFFQSDIPPQTYSSVLAPQTTGPQNNQPNESEKVVKQFRSEMNKLLVFIGVALSFTGYDIYKRLFAENTEYVSEETLAHIEKAIEAETNNRIDDAISHYIDALKQLDSEELEHISPCYTSCSVRIAELFEHEKKFDKALLIYKELSNSYLNAFTHREDFDALSDDSSYDFAILRSLTIAIRYAYLLPAKDVDLARETLMFHIVEAQKRVIEAYPPFLSVLNDINNRNVLDLITADLEKTIAHLPESEQEKIIKQQAKTPIELPLFTTEQTPENKILGLHVKAWPVFTRVLINAKDLYANLSIEANDLSAAISNLTSNSIIIQRCFDHPSRLTLTLTKLGVALQMTYQTISRDFPKGKEIEIIQDSEPVKVNLSSPELKDFVLNTTVSESKRIFLKVLVLCDTMKKQEKIIKANHLGQDIGQWEAMFKPALDKSEMVSAASLGMISYQSGFATDALKYFKRAKVLAFRLNDKDYIDDINEWIKLIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFC3</accession>
    <name>A0A1E3NFC3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74882</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015391.1">
        <property type="nucleotide sequence ID" value="XM_019164900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFC3"/>
    <dbReference type="GeneID" id="30181587"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="52" mass="5937" checksum="B17449C595A52D51" modified="2017-01-18" version="1">MVFTNGNIARSKKRLVEDLMNEEEHEPSLLTCDVSVREHHFGMSCTEPSTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFI3</accession>
    <name>A0A1E3NFI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Autophagy-related E2-like conjugation enzyme ATG3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_26723</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016018.1">
        <property type="nucleotide sequence ID" value="XM_019162461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFI3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFI3"/>
    <dbReference type="GeneID" id="30179148"/>
    <dbReference type="OrthoDB" id="1584384at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="83"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="315" mass="35558" checksum="3880DFE34EE3DB80" modified="2017-01-18" version="1" fragment="single">LRSTFSSLREYLTPITHKSTFATTGEITPDEFVEAGNYLVYRFPTWQWSPAEASKAKDFLPAEKQFLVTRHVPCYVRAADYEYNDEEDDDDGKDGSGNTEGSGKLKVSGAEEVSEDEDIDDIDDLIDENAEDVEFVDNKVSRKTPEGSKKRSYDLYITYSTSYRVPKMYLVGFDSNSVPLTPEQMFEDIASDYRHKTVTIEKAPFLNNTTAVSIHPCKHATVMKVLMKRAAASARERSHSKDTEKMMKKISHLGLKDHEGDDSHAAEDGDDWEDLKGESSEGDEGIRVDQYLVVFLKFISSVIPGIEHDYTMEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFN6</accession>
    <name>A0A1E3NFN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase family 15 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73750</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016063.1">
        <property type="nucleotide sequence ID" value="XM_019164573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFN6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFN6"/>
    <dbReference type="GeneID" id="30181260"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF10">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="51495" checksum="D4337E10CFFEE944" modified="2017-01-18" version="1">MTLLFKGLLFFIAAQVVVYSLFFKPSISSKEIRGIFEESKSRINSRFNETSKYLNRAKIKGASNSYIEPVYMNDLNHTVLTEENIRLENATILVLCRNWELEDILSSMRSLEDRFNKDYHYPWTFLNDVPFDDDFIQWTTEMASGKTEYGLIPAQDWNTPSFINQTKYDEGIEEAISRQILYGFSKSYRNMCHFNSGFFFRQELVNQYDYYFRVEPGVEYFCDFQMDPFRLMREQRKKYSFVITLYEYPDTIPTLWETVEDFFNEYPQHLHPNNSAEFITDKSPIGTNALDLGETSYNLCHFWSNFEIGDLNFFRSQAYLDYFNYLSKTGGFYYERWGDAPVHSIAAAMLLDKNEIFHFEDIGYTHVPIFSYPESKQIKKYKRCIIPKDERNLNVGLASCLPRWWKIGGGKRFLKEYYHEEEYIAYKEHNKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG56</accession>
    <name>A0A1E3NG56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_166095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016205.1">
        <property type="nucleotide sequence ID" value="XM_019161276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG56"/>
    <dbReference type="GeneID" id="30177963"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="301" mass="32075" checksum="CA8A2F7D38F1CECC" modified="2017-01-18" version="1">MNVWTGAVCLCVCVRWRRDKTEGGLFVGANSAVANAVAAAAAAAAAGCRCRVLADKQDVSSRDGCDVGLTGMKRWALAAALCCSADPHTPVHPLSRPPPFPSSLSFTGPPPSLCLQSARLLLFLSGPPHRARCGSGDFSRSRPSAAPAAGAVLRPAAYIWRRPRAVCCCTCACTCHHYDRYCCCACACFLVGLPTPLFRKPAPRTSPHRRSAPLRLPGACADCTSLRLADLLLLLVPASLFCCAAPLQARPHPGAALLCSSRPLFRCAAQKWSHKRKMPEMRKAKSKKGKTGRLCKPLQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG68</accession>
    <name>A0A1E3NG68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_35715</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016250.1">
        <property type="nucleotide sequence ID" value="XM_019162960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG68"/>
    <dbReference type="STRING" id="763406.A0A1E3NG68"/>
    <dbReference type="GeneID" id="30179647"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="458" mass="50332" checksum="497F283B32CE8299" modified="2017-01-18" version="1">MIDASMGEIMVTLEKINWILKHGDSILEPSKRPGPANMLMKYKNAQVWYEPLGVVGAVVSWNYPFHNLMGPIIAGVFSGNGVVIKCSERVRWSSEFFMSVVKGALKICEIDEDLVQLVCCWGKDADAVSGNPRLDHLTFIGSRPVAKKVVKKASDILIPVVVELGGKDAVIVCDDYMKQKGVDSIASTLLRGTFQSSGQNCIGIERVIVCGDRNNYELLLTKLKEKIDMFHLGSDIDQGEEIDMGAMIMGGAKFDELEGWVSNIEEQGKGRVLCGGKRYVHPNYPQGHYFQPTLIADLDPNSAIAQNEVFGPILSVFHAADDEQGLEIANNSEFGLGGAIFTTNRKKGFEMTRRFRTGNVAINDFATFYVCQLPFGGIKGSGYGKFGGEEGLRGLCNEKSVCFDKSQFISTSIPGVIDYPIKSGKKAWTFVSSMNKAAYDYSLFERVKGIYNLAKNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGB4</accession>
    <name>A0A1E3NGB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TECPR1-like DysF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73930</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016265.1">
        <property type="nucleotide sequence ID" value="XM_019164624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGB4"/>
    <dbReference type="STRING" id="763406.A0A1E3NGB4"/>
    <dbReference type="GeneID" id="30181311"/>
    <dbReference type="OrthoDB" id="3993429at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="7">
        <location>
            <begin position="116"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="608" mass="70438" checksum="CABF3A960A8D6793" modified="2017-01-18" version="1">MPKVEDLMGRSVSINSGPNEIETSKKKNQLVRLMLKFAISVMMQGEPNIDLLLTDSDSYNGISSEKRKRRGHFIDCFFNETLFSSSQPQTNDTEMSTLQVKSNENKSSTRKKKQTLSLRQIAINFHLLSKRLHVITSFMQNAAGVVNWERNDKTLAILLLYTWACIHPHFFLIYPIILLIYYISGKYLTKHPVVEKPSMYVMNEEYSNHWDNGLGPRLKNTRANSVHGIFGFLWEVDDKKNKDSELRQSYGTQVLGNGRPFSATMSGDEDELDFQTVDDMLTFIEEAVANENNEPHLLLRRQLKKKSLVDDATKVNVEIEPKGGQIFYRNFIMKTLFDIQIQTTQILDHFDNIQLAVAENCEFIDEKESTLLLFKLFLIVSIGCILGSYIPWKTIFIASVWVGICMNHPKRKVLIDKIMGPTTEFDNINQNGEKGSDHDTNEAQVGLFSVDNVVINEPVFAREVQIFELQQQDIMNPNDYVPLGFTTCVFCISEDIRLQRRKPACANDIGDVIPPLKHEKDEAYSGNQINKENRGNDKFRYDNRKKKSVLYWDYISGETWRLSDTKWVNNVRSSQELYVRPCEDEGWVYDITGEFRRRRWVRRIFQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGE9</accession>
    <name>A0A1E3NGE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016324.1">
        <property type="nucleotide sequence ID" value="XM_019164640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGE9"/>
    <dbReference type="GeneID" id="30181327"/>
    <dbReference type="OrthoDB" id="60822at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417:SF2">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="198" mass="21509" checksum="6A6E217FCA56A814" modified="2017-01-18" version="1">MASDMASDTTQLQDPVAYPLVSILYCVKCNWLLRASWYQQELLQTFTSKATSADEAVVHSIVLRPSFVPGTFQVLVKRSADAEWVLVWDRLRDGGFPEAKALKQCIRDVVSPERSLGHSDKKSSGTLLTGSKASSASSPAPPPASGAVSSTGSIPLATPREHTPTEREIETYGSPKLASSHKTFCEECVENLPPAWEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGH7</accession>
    <name>A0A1E3NGH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_36069</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016368.1">
        <property type="nucleotide sequence ID" value="XM_019162982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGH7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGH7"/>
    <dbReference type="GeneID" id="30179669"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="299" mass="34218" checksum="D54634B66DCFE340" modified="2017-01-18" version="1">MFRAFSRNIHTITSGGKYTLEFKKYLADAKGRPQSFFHDVPLNYDRAEGTVNMVVEIPRYEQGKFEISKELPLNPIVQDTKKGKPRFINNIYPFHGYPCNYGAVPQTWEDPTLAIDVDGKQYYGDNDPLDICEIGSNVDQAATVGTLKRVKILGCLAMIDDGEMDWKLLAIDTADKLAPHLHDVPDVEEKMPLLLENLTRWFKNYKLPMNKPENEFAFQGRWLGRARALDVIDECHHRWQALVAGSHTGKKFPLIANETLPETPGFQTVQQLETVKTVFENNGSDHVPLEAREIFYYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGJ2</accession>
    <name>A0A1E3NGJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2-oxoglutarate reductase</fullName>
            <ecNumber evidence="4">1.1.1.399</ecNumber>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_74032</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 18">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.399" evidence="4"/>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016374.1">
        <property type="nucleotide sequence ID" value="XM_019164656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGJ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGJ2"/>
    <dbReference type="GeneID" id="30181343"/>
    <dbReference type="OrthoDB" id="1621027at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061759">
        <property type="term" value="F:2-oxoglutarate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04901">
        <property type="entry name" value="ACT_3PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12176">
        <property type="entry name" value="PGDH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000036">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008759">
        <property type="entry name" value="PRK11790.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="398"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="468" mass="51201" checksum="65EC81D647ADD207" modified="2017-01-18" version="1">MSIPQPISNLTQAFERTGLSPKNNNTVSTSPTASYFEQHRLAGGRRAKTLKPFSTGDVKILLLENVNQTALDIFGQQGYQVEFLKTSLPEEELIEKIKDVHAIGIRSKTRLTANVLKHAKNLVVIGCFCIGTNQVDLEYAAKNGIAVFNSPFSNSRSVAELTIAEIIALSRELGDRNMELHNGTWNKKSARCWEVRGKTLGIVGYGHIGSQLSVLAESLGMNVLYYDTLMIMSLGNSKQVGSLQELLNSSDFVTLHVPETAETKQMISTPQLAEMRDGAYLINNSRGTVVDLPALIEALRIGKLAGACIDVYPKEPRANGPYFNGHELLPFIDELKTLKNVILTPHIGGSTEEAQSAIGIEVGTVLTKYLNEGASIGAVNFPEVSLRALDLDQENACRILYIHQNVPGVLKTVNQILSDYNIEKQFSDSRGDIAYLMADVSGVNSDDIKNLSAELEATAFNISTRLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGL1</accession>
    <name>A0A1E3NGL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar protein sorting-associated protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74023</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016365.1">
        <property type="nucleotide sequence ID" value="XM_019164650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGL1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGL1"/>
    <dbReference type="GeneID" id="30181337"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="168"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25903" checksum="C77392B7FC760D67" modified="2017-01-18" version="1">MGNTSSNPKITRHDKAILELKLQRDKLLKSRQRLATVVNRETQIAKQCVKAKKFEKAKLALRKKKRQQSLLDNLERQSNTLEELIDTIEFKLIEKDVLYGLEQGNKVLKEINSEMSMEKVEKILDDTEEAVSYQNELSERLGSLLTNGEEEEVDEELRQLELEMGVADRQESVSVPKKAQSDSAVRRKLDSLPAAPSELHLPQKEAEGDGAPQPEKQEEETSRVAQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGM5</accession>
    <name>A0A1E3NGM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="7">2.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_18391</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + acetyl-CoA = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:17017"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="2.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="7">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="7"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015606.1">
        <property type="nucleotide sequence ID" value="XM_019161984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGM5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGM5"/>
    <dbReference type="GeneID" id="30178671"/>
    <dbReference type="OrthoDB" id="537444at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006257">
        <property type="entry name" value="LAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01349">
        <property type="entry name" value="PDHac_trf_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 7" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="42"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="10">
        <location>
            <begin position="186"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="136"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="481" mass="50662" checksum="308CC4EC8C431BA1" modified="2017-01-18" version="1">MSSILITRSTPLLRLSSRYAARRLPSLAQQSFRHYASKFPEHTVIDMPALSPTMTEGNLVKWYKKVGDKLAPGEAIAEVETDKAAMDFEFQDDGYLAKILVPDGTNEIPVNKPIAVYVEDEADVAAFESFTAADAGDSTTAAPAAAPAAESKSEPAPAAAEKSEASKPAKTGTSGSAASAQTDRIIASPLAKKIALDKGISLKGVKGTGPHGRITAKDVESIKAAPVAASVGTASASAAGAAAGSPAASYTDIPLTNMRKVISKRLTASKQGAPDYIVSSSISVSKLLKLRKSLNASADGRYKLTINDLLVKAIAKAALRVPESNSSYLEDKDVIRQYSTVDLSIAVSTPTGLITPIVKNADTKGLKSISTEIKELGKKAKDNKLKPEEFQGGTITISNLGMNHAVSMFTSILNPPQAVILAVGTVEKKAVPDKANQFGFVFDDVMTITGTFDHRVIDGAKGGDFMRELKTIVENPLEMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGY5</accession>
    <name>A0A1E3NGY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nas2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015941.1">
        <property type="nucleotide sequence ID" value="XM_019164792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGY5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGY5"/>
    <dbReference type="GeneID" id="30181479"/>
    <dbReference type="OrthoDB" id="72325at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nas2 N-terminal" evidence="1">
        <location>
            <begin position="28"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="109" mass="12677" checksum="8D805AFBA379877E" modified="2017-01-18" version="1">MSVTNQLPQTTYTLNITSRGQLNAMSFEELSKYRKELDEDLSFLFSYLKNTLHADMDTPLLSGDGFPRSDIDIVQVRLCRVKIIKLQNDYKWISETLLDKMQQQLAKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH23</accession>
    <name>A0A1E3NH23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74184</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016537.1">
        <property type="nucleotide sequence ID" value="XM_019164699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH23"/>
    <dbReference type="STRING" id="763406.A0A1E3NH23"/>
    <dbReference type="GeneID" id="30181386"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051698">
        <property type="term" value="F:saccharopine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF26">
        <property type="entry name" value="L-SACCHAROPINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="8"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="436" mass="48865" checksum="516CD1E3F3AC965A" modified="2017-01-18" version="1">MSAEDKTVLIVGCGIFGLASALDFAKAGYKVTAMDAYEPPSPWSASCDYNKIIRGEYDVEEYTKLSVEAIEMWKNEPRFKGIYKDCGRVMITPTDYKARQEFERVGIENLQKHGKALDIEYFDGGEELARKFPFLKYNGLQKGEQSKFNPYGGLGHSSNAMVAVYKEAKDHGVQFVFGDDGHAVEVERGDGNKVVIVTKSGQRRTAGTVIVSLGSNTGRLVNLNGQQSATGLFVTFIKLTDAEYERYKDCSILFDAKMGYFFPPDPETKLLKVALPGSGTCHKVHDPHAAVSEDRKISLPRFKNLNPTDTMPVHGEREAKLLLAKYVPELAYHELLHSKICWIGDTDDSNFIIDRVPGFTNLFVASGDSGHAFKFLPNIGKYILQRVKGELDPVLTRLWRWKNGSGFDPAKCSWRVQSEYPDVSEIDFYKELKAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH82</accession>
    <name>A0A1E3NH82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_74238</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016592.1">
        <property type="nucleotide sequence ID" value="XM_019164716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH82"/>
    <dbReference type="STRING" id="763406.A0A1E3NH82"/>
    <dbReference type="GeneID" id="30181403"/>
    <dbReference type="OrthoDB" id="10249309at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900036">
        <property type="term" value="P:positive regulation of cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1070:FF:000002">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="305" mass="33920" checksum="B1CA950B884A620F" modified="2017-01-18" version="1">MPDFDIKQTYLKFLEDDPEMTMPVAAIESLLTMLKVKQPSTSSELIRLLSDATTELKASVKNSVSLSAGCDLFTRFVLRNTHLYSDWESCKKHLVENGQLFLSRAKESRDMIAKIGFHLIKDDDTVLIHSYSRVVLSLLAYSKSKFVRFRVFVTEARPGSDDGKNGKDMVDALKSLGIPSCFIHDSQVGFVVNKVDKVFMGAEGVSESGGIINHVGSYQIGVLAKNANKPLYVVSESHKFVRMFPLSPDDVPGNKLEFTTNPEIGLLEDANIDFTPHQYITALVSDLGVLTPSAVSEELIKMWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHG1</accession>
    <name>A0A1E3NHG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_23434</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016677.1">
        <property type="nucleotide sequence ID" value="XM_019162307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHG1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHG1"/>
    <dbReference type="GeneID" id="30178994"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="3" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="19"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="292" mass="31465" checksum="6A7B8DE5AABA3F34" modified="2017-01-18" version="1" fragment="single">SLLLSFTLSTVVSAISDYNNVALYWGQNSAGTQQDLASYCSSEKADIYILSFIDVFGSSQQPNVNFANACTESYSSGLLHCPQIGKDIKTCQSLGKTVLISLGGASGAYGFSDDSTADFAGTLWNMFGGGSDSSVERPFDDAVVDGFDFDIENNNPKGYATLANKLRSYFSEDSSKTYYLSAAPQCPYPDASVGDLLENASIDFAFIQFYNNYCATTGSNFNWDTWNTFAENTSPNKNIKLFVGLPAATGAAGSGYATIDQIKSTFQNTILNKEDSHFGGFVLWDASWSTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHI5</accession>
    <name>A0A1E3NHI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH-ubiquinone oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18185</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the complex I NDUFA8 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015916.1">
        <property type="nucleotide sequence ID" value="XM_019161907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHI5"/>
    <dbReference type="STRING" id="763406.A0A1E3NHI5"/>
    <dbReference type="GeneID" id="30178594"/>
    <dbReference type="OrthoDB" id="276296at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016680">
        <property type="entry name" value="NDUFA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13344:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13344">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017016">
        <property type="entry name" value="NDUA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="182" mass="20558" checksum="BDBAC0A3260E9224" modified="2017-01-18" version="1">MSTSNTYDTISTSNFTDKKKGTEYVFENDKLPEEIPHVKEIGATTGPLLSASFFIADRCQDFNDDFMLCQKTNGTNGTVNCLQEGRKVTRCAASVLADLNAHCKKEFEMHYKCLNYSNMEFKNCRKAEHLLNDCVFTSLKLAKKIPGDGGREVFKEGHQIYKPIHPHFPSEKAYDLAKKESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHK6</accession>
    <name>A0A1E3NHK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ribosomal subunit protein uS4 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18202</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015936.1">
        <property type="nucleotide sequence ID" value="XM_019161912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHK6"/>
    <dbReference type="STRING" id="763406.A0A1E3NHK6"/>
    <dbReference type="GeneID" id="30178599"/>
    <dbReference type="OrthoDB" id="1697570at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000021">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="190" mass="21807" checksum="A262464D9E06C7C1" modified="2017-01-18" version="1">MPRAPRNYSKTYSVPKRPFETARLDGELKLAGEYGLKNKKEIYRIGFQLSKLRRAARDLLTRDEKDPKRLFEGNALIRRLVRVGILSEDKMKLDYVLSLKIEDFLERRLQTQVFKLGLARSVHHARVLITQRHIAVGKQIVSIPSFMVRLDSQKHIEFASTSPYGGGRAGRVKRKSQAAKSEEGAEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHL1</accession>
    <name>A0A1E3NHL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_12099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016753.1">
        <property type="nucleotide sequence ID" value="XM_019159880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHL1"/>
    <dbReference type="GeneID" id="30176567"/>
    <dbReference type="OrthoDB" id="3992489at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1037" mass="121424" checksum="9ED57B51DFB0AB4E" modified="2017-01-18" version="1">MDEIHKKKVLEVPVNERNGKLKQLRFPDKTGDSKSHIHVNYAAYLEYINKNYPDLHTFDIGIDNETGPNREKIQKQLKNDFMLLNQLDIEQYEGLNSPDDIWDSLANSVYGMGFIGRYSRIATSLETGINYIFTFEDFAHIYPFLKSCQGNALLIRRLSGLKDVENKKFSSYKLYYKSQPEHSFGAANSPGVADVELERRSIYNHSFVIKPEFDNPPGTEWSYLSVDKDKVEMGNSYRRGYSYVPDTTPVELDAGLGFRHELGENSPTHETPECAEQPELETEKVLADSSHDNRRYIYEKYLFCTYKWCPGSMRIILDCIHNVVILSMTNPHFDCHESHKRKIYDIVRKLFVLNQGDIDLIDAKYATIPRRYGLAFIESWIHSEEFELARSSEYTPSFQSYNINESDQSKSWASQKNRNWVVDSFYNADSAKSNSTLTINGQGQHKRKATHVKTKQDYVAHPQRLIDLRNYGIEYWDFDIFKYDFFGNVTPRSEWEFHVNRDSDEKNREVFRFWRKQYFGISDFVRQTSNHLSIGKSFGLVSYFMANNGEHTFQKKKVHIYSFGDQLHVKQQGQIKKDFLKDIYLFTRNYDWEKFDSILFKQTSVASTLPNPDLHILPYPLNRQVQKLSETFGEAGLLGDHESQSGCVFVCSSIRTIANSDKVVICERDIKYQDIILEEWRALHRRKSMIHPSDQIWNMEPNFIQALGMMAIYDKQFLKNRHIVDRFNRLRWKSMNDQYYLKTLGDALADEGCKYRSPQINRRRLPAKPVSESEFRYVGFRVLYETNGQVETGYIFCSENKLSSSLKLMLEQRTIQEHLPMVLLVDDSKCNPEQLQEIQDKVKIVQVRSFLFGKLKSLIPFDYLLFLMRALIMENVDMDTGEKLLKKRLPPQRKKDVEFYKLEFEGRRIASNAGLVRIAVQKLCKVNSYLTRYQDAWRIGKRWGSFDLAIRASGEDKWKFLPKSLPNFDNLQFAKMLHEAGRNEKNIQLAEDVESVKLLEWAIECVKSEEQKNSCEELVAGEFRDFFELIKRTTDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHY9</accession>
    <name>A0A1E3NHY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_78246</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016878.1">
        <property type="nucleotide sequence ID" value="XM_019164961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHY9"/>
    <dbReference type="STRING" id="763406.A0A1E3NHY9"/>
    <dbReference type="GeneID" id="30181648"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044280">
        <property type="entry name" value="Hac1/HY5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714:SF6">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0834">Unfolded protein response</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="42"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="310" mass="34399" checksum="DA8243515BEA15F2" modified="2017-01-18" version="1">MSSPLSDNSNSSCKLSAELIDVNSNGSHLPPRKRARTEAEKEQRRVERIIRNRKAAHASREKKRKHVEQLESYVKVLEAHLNLSLEANRTLLSKLAANNIPCDGVDIKKLQVPRPDGLLLSDEDETSASNNSNSTPDDHVADSIIAQRECRIGQSSKCVDAEEVDFNNDLELEAEQEDDEADSSVVDEMPVLSKSSPSSSVSASEFSNPPTPGNDENLFSLYRDPRDFNLCITDSNDFMDLNSYLEPEFSTSDFNEIDQSLDFKSSNDNIDSNGQNTMGYLSSYNSVHSAAPLSTEINNAPFDLDEFIMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI00</accession>
    <name>A0A1E3NI00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73761</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016076.1">
        <property type="nucleotide sequence ID" value="XM_019164578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI00"/>
    <dbReference type="STRING" id="763406.A0A1E3NI00"/>
    <dbReference type="GeneID" id="30181265"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF21">
        <property type="entry name" value="AROMATIC_AMINOADIPATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="80"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="449" mass="51073" checksum="484D94E9EBCA0973" modified="2017-01-18" version="1">MTRDWTEFLSEEALLRKRSVMAFPKGDVISLAVGLPNPACFPLKGVELDVESPGSQFRKTERIGYTIDTHPDQLVDSCQYMSSRGLAHFDEWVKAYVEKYFLPGYAGWKYLVQSGATQSLDAIFRMLLNPNEDTVLCEKLTYSCFLETCIPFRVKLFSVEMDDFGVVPEKMDELLCGWSAAGSATEGLKKPKVFYTMPTGHNPTGITLSTERRRALMEVCNKHNILVIEDDPYYHLQLDDAANIPSLLKFDVEGRVIRIDSFSKMLMPGLRVSIVTANDLFIDKLSMHNELSIHSASANSQLVLEMIFSKWGDGGFDAWLRHLQSMYRRRRDVMLGAFDRYLPKSLVTYNRPNHGMFIWVNAKLDQFAQPSDSTLKDYEWATHVEDEIFRVASEKYKVILTKGHWFMQDKHTVCSGFRATYAFASEDEMVKGAELFGKAISDVHSSLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI18</accession>
    <name>A0A1E3NI18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar sorting protein Vps3844 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016908.1">
        <property type="nucleotide sequence ID" value="XM_019161450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI18"/>
    <dbReference type="STRING" id="763406.A0A1E3NI18"/>
    <dbReference type="GeneID" id="30178137"/>
    <dbReference type="OrthoDB" id="5583277at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053065">
        <property type="entry name" value="Archenteron_Induction-Rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024382">
        <property type="entry name" value="Vps3844_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853:SF1">
        <property type="entry name" value="DUF3844 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12955">
        <property type="entry name" value="Vps3844_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133378" description="Vacuolar sorting protein Vps3844 C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting protein Vps3844 C-terminal" evidence="2">
        <location>
            <begin position="334"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="436" mass="48599" checksum="C7AA9242EA7FE104" modified="2017-01-18" version="1">MRFSALGTAIGGLAMAKCVAGIENAAVYKLKNNDCQSHGLTIGQTDATLNLAYDFGVSQFYNVEDVEDLNNVVLGNRKAEDGEEEKKLILIINGVEHPSDFFGSYDIAPTFDVEIKDDRHSSLLKSFLQDVPNKLYTLNKNSGYYLNKLSNEITILTDSNKKASYLKNLWNKYFHQEETNKVEGLWELLKDSVTLHPKAQKGENVLKINKRSMDYINDESFISELTQLEFFLNDELENNIKNDKVIINIDSLISIFKKTGVTQTYETCKNIISKVVIEKLQNHGDVSATIVVLPLDQSLVTVKGTEVFLKQQQHVSSLHRRSTESVFVKTSNACFANQLACIESTDSCSAHGLCTLVGSCWKCLCSATTDDKDRTSYWTGASCEKADYSSQFNLLLWTTIVLILSIVAGIKLMYQCGEQELPGVLLAATVQTKKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI58</accession>
    <name>A0A1E3NI58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_59451</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016948.1">
        <property type="nucleotide sequence ID" value="XM_019163369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI58"/>
    <dbReference type="STRING" id="763406.A0A1E3NI58"/>
    <dbReference type="GeneID" id="30180056"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF41">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="34"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="485" mass="53692" checksum="EEA4487DFACCE30A" modified="2017-01-18" version="1">MDAAAKYLAENEGKYEPMSETEMKKMVRKTDLFLIPVLLITATMGAVDKVALGTAAIFGLRTDNNLVGQQYSWLSTILFIGSLVGMWPMSYLIQRYKLGKVIGMCSLTWSSLTLLLCACHNFGGLAAIRFLMGFVECAIVPGCSLMVSVFYVKHEIPHRTAIVFIFMSSVINGALSALASTFGNHIPTWKYIFILIGSVSVVWSVFVLWFLPDSPMNAKFLTDREKYYAVKRVAENKTGVNNDEWKWAQVREGFLDVRMYIVMLFNFGINIPNGGLSAFSSIIIRNLGFSSVKSSLMGIPTGVIASIATVFFSWLAARWKNRRCLLAIISLVIPLIGSIISYTTPHSNTAAQLVGLYFQYFYFASYVVMISCVQANTAGSTKKAITYSFNYLGYCGGAITGTQTFRANQAPRYTGGFISMLVGYCACMLLSGTYWLVCVVLNRRKKAALQTLSGDSEAVFEVSGVEEEEVADLTDKQQAHFFYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI95</accession>
    <name>A0A1E3NI95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016954.1">
        <property type="nucleotide sequence ID" value="XM_019164451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI95"/>
    <dbReference type="STRING" id="763406.A0A1E3NI95"/>
    <dbReference type="GeneID" id="30181138"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF36">
        <property type="entry name" value="GLYCOGEN-BINDING SUBUNIT 76A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="345"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="665" mass="73427" checksum="472640EA07EF23C8" modified="2017-01-18" version="1">MSYLGPKSFSTAPLTSSNLAFLNDLSSSELHPSKNNASLHDSRKDNNFITDSNKTMDDESQNKPPTGTQPTSPSLLESEANKEESETINDKTHASTDSSSNSLSAFSTISTTNTPKSSETSIHSLGESHSHRTLIDLNEEDNKTVILTSTNNTVSNNSPNANESQLIIPSCPMKTYDLVSVSTSTDSVNDYNNNIHNSNALFDVESISNPSRFPLLRKKSGELVKSSLKLNSLQRSNSMPNAKSVRFASRLENVKFFKKSERPTAVSSRFAAPPRTHWEFDSNSSSDNDDSGFRHDDDGEDDAENGFKLNESYEDFLSDDEIDSASRWIITSNDCPYNPVSLNFAKLGSNNNVILESVKLNSSGNSLIGFVYVKNIAFTKKIVVKLTYDNWNSFIEIENANYISSNHIFKYSDSIANNYDKFSFIIKLDNLNTFGNNLTLIFCVQYLANSISFWDNNNGSNYKIVLRKANSIQSGDNIGVDNSIKNKKMIGRKNFSNLLDIEDSNIKLKDNNNFMNSSSCKPLTTDMSSLRISNSFGLKKIRSESSIPSMKSNILSEFNNKSTSSLSNSKSEDPAPVAIKQTKVQFSKSANASPALNSPAFYPSPKEFNDYDHIVKQFCFFSTSDQKKSGGGVGTGLCATNSINEQNYLDGFNHSKSQASLPVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIC2</accession>
    <name>A0A1E3NIC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_35848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016206.1">
        <property type="nucleotide sequence ID" value="XM_019162970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIC2"/>
    <dbReference type="STRING" id="763406.A0A1E3NIC2"/>
    <dbReference type="GeneID" id="30179657"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000516">
        <property type="entry name" value="FAD-dependent oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="497" mass="54930" checksum="9FCF1EE2066C52BC" modified="2017-01-18" version="1">MPTTTNNVPEEPEFITYSTFPSGKHQGKTHYIIVGAGIIGVCIAFYLTRHPKFDPKKHYITILESKRVAGGASGKAGGLLALWAFPQQIVPLSFQLHQDLANEFGGEDEWGYRRLTTISVEGDISDARLNYIQDKPMSRATTIKKNSIEIESNDSSAEKRSSHLPKNLMWIDDKVINDWNQLGNESTTAQVHPYQFTTFILKQCIATGCVELVIGKIDDIIIDEESGECNGIIYHPSSAKAENEGLTKQKFELHGDKVIMSIGPWTSKILPDCPISGLRAHSIIIQPERVEEISPYAVFTELKLNKNKFVSPEIYARKDEVYVCGEGDSSVLIPETTDDVEVVTEKCEELFKYVSKLSNNLKNGKVLKRQACYLPVLDIPSSSGPLIGETNVDNLYLASGHSCWGINNAPATGLLMSELLLDGEATSCDISALNPSLYFDATLIYDEEEADLENDDDEDAGSNASEKGSYEDSYDDDDGAEEDDDEDEQDEQDGNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIH3</accession>
    <name>A0A1E3NIH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Asp/Glu/hydantoin racemase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_34341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HyuE racemase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017027.1">
        <property type="nucleotide sequence ID" value="XM_019162878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NIH3"/>
    <dbReference type="GeneID" id="30179565"/>
    <dbReference type="OrthoDB" id="412018at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052186">
        <property type="entry name" value="Hydantoin_racemase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053714">
        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047:SF5">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="Aspartate/glutamate racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26264" checksum="E5B5012D35135611" modified="2017-01-18" version="1">MVAILVLNPNSTDAMTRSLQSEVESLNLPSSYQIDYYTGPVAKSPPSINNQEDADCSTKACIEDFDNRCPHIYKVYDGYLIACYSDHPLVNELQTKITRSGVVVMGIFQASMLYAMNFASQNNKAAILTSGHDWEPLLDRAITKFCANDRGSFPATKFIPTLAAGIPVLDLHKPENYPHLKDCIQQLVENDVGIILLGCAGLSSLSKRMKSEYPHLKFVDSVKVGARLLISYIEVGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIP6</accession>
    <name>A0A1E3NIP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_25348</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016571.1">
        <property type="nucleotide sequence ID" value="XM_019162388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIP6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIP6"/>
    <dbReference type="GeneID" id="30179075"/>
    <dbReference type="OrthoDB" id="5592486at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="2">
        <location>
            <begin position="75"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="267" mass="30201" checksum="AE7CFCFE68EF10B2" modified="2017-01-18" version="1" fragment="single">PRYPLVLCHGFSGFDQMLQLPGKYLIEYWNGVKSNLEAHGCTVLVAKVPPFESIEHRASMLDKFIRKSVPQIRLRHHIPKDEPVKLNLVAHSMGGLDCRYLIFQQQNSAEPRPPYKIMSLTTISTPHRGTSAADFALRYFPASLIRSNFPSIFQLSTKYAGKFNSVVTNDPTVKYFSYGAQMHPYPGSIFLASWKIISDREGPNDGMVSLKSCQWGHFVGYLDDVDHADLINWMGLVKKTKMALGIPNFNPKYFYLEIADNLAKNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIS2</accession>
    <name>A0A1E3NIS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016366.1">
        <property type="nucleotide sequence ID" value="XM_019164651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIS2"/>
    <dbReference type="STRING" id="763406.A0A1E3NIS2"/>
    <dbReference type="GeneID" id="30181338"/>
    <dbReference type="OrthoDB" id="3366546at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF26">
        <property type="entry name" value="PROTEIN TMA23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22793" checksum="70BE1FBE29BC67FB" modified="2017-01-18" version="1">MNSQKYLESYGWTPGQPLQKGGIKKPILVSHKFDLKGVGHGAKENVSWWETLFDGHLKSLDVNGKDGFGADNAKREALERAEKKKQSPLYQMFVKGEVLRGTAELPESSVGATGAAAKRKAEAEQPEQLMFFFSSDDESDGESRQRQKRAKKEKKVKDNKEKKEKKEKKEKKDKKDKKDKKREKLEKKARKDKKRQKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJG7</accession>
    <name>A0A1E3NJG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_73233</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016831.1">
        <property type="nucleotide sequence ID" value="XM_019164418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJG7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJG7"/>
    <dbReference type="GeneID" id="30181105"/>
    <dbReference type="OrthoDB" id="2996783at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF4">
        <property type="entry name" value="PEROXIREDOXIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="11"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="7">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="232" mass="25834" checksum="5C8EBD25C6494F5E" modified="2017-01-18" version="1">MSIPEPKNGTLRLGSTAPNFTTDTSVGEVTFYDYVGKDSWCILFSHPDDFTPVCTTELGAFAKLEPEFAKRNAKLIGLSVNDSDSHRAWIRDIDEITGSRLQFPIIADPERKIALLYDMIDYQDASNIDDKGVQLTIRSVFIIDPAKKIRLIMTYPASCGRNTAEVLRALDSLQTSDKLSRKITTPINWVPGDDVIIAPSLSDAEARALFPKFRAIKPYLRLTPLSKDLSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJK4</accession>
    <name>A0A1E3NJK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16225</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat rae1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017438.1">
        <property type="nucleotide sequence ID" value="XM_019161127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJK4"/>
    <dbReference type="STRING" id="763406.A0A1E3NJK4"/>
    <dbReference type="GeneID" id="30177814"/>
    <dbReference type="OrthoDB" id="256303at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000728">
        <property type="term" value="P:regulation of mRNA export from nucleus in response to heat stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000190">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="40761" checksum="B65B67B2D35D3548" modified="2017-01-18" version="1">MSFGSRLGGFGANVSTLGNTTTSASGTLQDLQNDIVVNNIGDDSVSDIAFSPQAEFMAISNWDKKNRIFEITNNGQVEGRALYEHEGPIMSTRFTLDGTKVISGGADKQVKLFDLASQQAQTIGVHNDTIRVVRHVTCGPQNTPCVVSGSWDKTLRYWDTRQQTPICTLEMPERVYTMDASQKLLVVGTAERHVVIINLDNPDKIFKSSISPLKYQTRCIGCYPKGDGFAIGSIEGRCGIQYIDDMQQKQSGFSFKCQRVNKTTTKETNIYSLNSVVFNPQFGTFATAGSDGSFSFWDKDSRHRLKGYPQLGATIPVVNFNRTGMLFAYALSYDWSKGYEGNTPNYTNTVRIHVCKEDEVKPKPKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJQ1</accession>
    <name>A0A1E3NJQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FIST domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_12033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016686.1">
        <property type="nucleotide sequence ID" value="XM_019159872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJQ1"/>
    <dbReference type="GeneID" id="30176559"/>
    <dbReference type="OrthoDB" id="4080562at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="44217" checksum="BFAA77085CBB4BA2" modified="2017-01-18" version="1">MLRFNRWVKPAFLLKRSLYTKTTLNAGGIEIAQPNPNHKSLILLTTPPQLNNIVSNVIDKQATNTGISQILVVCVDSIADSRNAYSELWLETPFRVTHHETIEERNERIKKKVEPLSVDPIKFDKSWKDTKEQTLFQINLTNLGLNSKVRLANTLFENGENSTCFYIGKESYDWYNLANVTVDVNLDNFSAVQNIEYANRLTEIPLVEAVEEGADEELFTITDFEGNLIKSINNQSASGYLTDNSIVMSSKKDLYFKLYDNETEDPTRPCLQEYYKLIVGGLGWGEKQAFLAVDPIVGTTGFRDVKLFYYDKDTAKHHVESPVVSGNSKLVFECSELESGYEEYKQPTDTGAQETVIKPGVFAMGCEQGFELDGIWHRSNGEFVQIEVAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJR8</accession>
    <name>A0A1E3NJR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_33593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017500.1">
        <property type="nucleotide sequence ID" value="XM_019162829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJR8"/>
    <dbReference type="GeneID" id="30179516"/>
    <dbReference type="OrthoDB" id="2125396at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="41327" checksum="2DECD975CC3A90F6" modified="2017-01-18" version="1">MEEEKLKLPLELITKILEYTPNPFCTNHLLLCRGIYWSFLWKVYSQPELVSNNLYPDESNNFVPKNLKLLMKRKFQNMVKCLDLSRVVQSGKNSYMSKLLRRTSSSLEIFISSQSSFGAAPLVSIKSCFRLKVLDLRLVSESVNLAELFKSIQCLPELEQLCFPRSSVTCDEFDFQWPQKLWYLRLQGGITDFFAANVRFPETITCLEFAHCSHLTRTGLDDILSHIGINLTRLSITYPMPKIGDDGADRSFWFCPNLKSFFVDIAYISWELFSEELLVILKEYDRPLRSMTIESTGYMGMCEKLSPNDITIAVDEERLPCFERLGLSAMLGWDFKSDDMNDMISELDHHNIEVFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJT6</accession>
    <name>A0A1E3NJT6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72457</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017494.1">
        <property type="nucleotide sequence ID" value="XM_019164204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJT6"/>
    <dbReference type="STRING" id="763406.A0A1E3NJT6"/>
    <dbReference type="GeneID" id="30180891"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="170"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="406" mass="42823" checksum="B535C8C85A69A8C9" modified="2017-01-18" version="1">MNDSRDHVSDSTTENMASSAASTSSSAGALALSSSITSLPSISTPLNDTVMGTLHPVKLFSLSSSSSLPTAASPQVPYTSVSHNPQGSFFITTSPNGSLQLFDSLRARSFKTTYSKKYGCSNAIFTLRTATHSVPTSCVIASTIPASSDDAANNALRFLDLNTNSFIRYFQAHTKQVTSTVSSPSSAFGLDAFYSASADGTVRAWDSRSDKCYACLANMGDSPIIALDRSGTILAIWHQNKSTVYLVPLESFPIGIIGKIEVANCPSERIEKIVWADKGLLILDPPGHDKIVIDTIKLGVAGTLSGVTPFAISSQSDDAARSGSVDVTPDGCWCLSGSGDGSTLAWNLSAFFGTGGRIPPVHPIRIANDELISKQIVPRILTINPKLGSVVTGDTEIVISIFNKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK19</accession>
    <name>A0A1E3NK19_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017574.1">
        <property type="nucleotide sequence ID" value="XM_019164224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK19"/>
    <dbReference type="GeneID" id="30180911"/>
    <dbReference type="OrthoDB" id="10333093at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="441" mass="50014" checksum="92BF59167EC4763B" modified="2017-01-18" version="1">MESDETICVVCQQGPTEYDPFVNPCHRCLVCFHEECLNLSIRTVANAIKDSKRIDPVNWLLWLFNRKVYEQLSTPRDPFSPGPIAIHYRQSLTCGFVDAKIRCTPSISDLLKLTSMRPNDTFTMLCPNCRASILVFGRKSVLSGVHKVVSHLKSFALATVSAGLVSGIALFSSFSMAATFAFSLNWASDFFQQTEEFDYTELLRFIMVPHFVYTMVTPNVGLLQLIFASIYSSFSYGIPASGITTTLQKFIYGKLITAITYRLTINRYYYESFKQTIPAVFGLKLSLDDAWAIQDYRNETVYEEYNTSPFWNKCISWVRDTWYCLWEDFGELYRVSALDVFFGNYGFMSTFAIGLLLGDGPMSNFLFNIYMTESQNQALSKLVGIAITQMAYFCVSTFNSCALANCYKNLKPGEGNMPVTKVRRAAEVVFQTFSARPTVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK23</accession>
    <name>A0A1E3NK23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein YTP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_24177</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016806.1">
        <property type="nucleotide sequence ID" value="XM_019162340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK23"/>
    <dbReference type="STRING" id="763406.A0A1E3NK23"/>
    <dbReference type="GeneID" id="30179027"/>
    <dbReference type="OrthoDB" id="4005299at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018825">
        <property type="entry name" value="DUF2427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10348">
        <property type="entry name" value="DUF2427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="530"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2427" evidence="3">
        <location>
            <begin position="27"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="4">
        <location>
            <begin position="247"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="61976" checksum="70E00E3604C9D170" modified="2017-01-18" version="1" fragment="single">PWAGGHAHGVPILQTKLTPEQQKYWESYNTTTYLTLESPYSGHIHAHLALLFISAFVLYPFVLVLNNLESNWFLPLLAVQASLSVVSCILYSVFINNVPDLYPNMAYTKMVTGWFVLTLLQALFAVVNSVRRWLNDFCSPSSTLFDDDNADTDSFNLDDDDNSASATMHGLGLNYNGNSTNNTLRSGSNSGSNSGSPLASTSQLTRSRNAVMQALSSSKALTVLSSSFGSLFTFLHSVTTWVLLAYFFVLFPTALSCLNLFGKGQRVFNLLAHFIKGGVFFTLGLLSLARYCGCFAGIGGGWNYAYIDLDRNKYGGLICTFESVESFLIFFYGCSNVFLEHLASTDGVWTAKDLQHVSIAFMYFGAGLCGLITEFKLSSWRRSLFFNVAGKQLATTENIQLDDNLTIDDQHHILGSNKMITPGFSPNPFPVFTIFWTGVLMSKHAQASALSSAIHVQWGSLLTYGSFFRVFTFFLMSYYPLHGSRECFLPSKPLTELVTSFCLLCGGMVFMESTDQVIEALAYRGLTPMFTINVSVGIISLLMAWVMFIFAVKDRMRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKE7</accession>
    <name>A0A1E3NKE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Exocyst complex component Sec10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16464</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017730.1">
        <property type="nucleotide sequence ID" value="XM_019161218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKE7"/>
    <dbReference type="GeneID" id="30177905"/>
    <dbReference type="OrthoDB" id="125856at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="7">
        <location>
            <begin position="55"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="6">
        <location>
            <begin position="178"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="457"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="909" mass="103494" checksum="694A3326F220A675" modified="2017-01-18" version="1">MSFGFVNTNQITLETFSSDTAPSEFVNKLSKLSEDTNGHDTYIHTDNNRVQYVDPVPYINSLRSVQDKLSLLMDECEKKKDVFERSTKKRENDHFTNVITQSTTAADLKSKFNDLLLTVEKLNTSKIDPLGEKLKKASTLKDNASNISFLLKCYNRFYINSEPPIELSTDRQRANTFETARILSQLLKLSSKLASDAKLSNAENAHTLILEFATTFESDQLKSFNTYYQSKNFARLQNITKTLFIYNNGINIVDFFVGGHPIFVQMQDNVKQPVSDSYWKTLRNPTSVDYSLDHVSAELLEAVRETILSEIDSITTIFQENAKQALISLIIKLVDNIVKPRMKLLLTTAMSQSTLCYLRILHLFYNGITQMAFAQLRSVLLDKSIDFGFEFDKIYNSLFNEHLKENSYFTLEKENLESLIDSLIGPFEDANRDAIKEKKLTMKIEKVKEDEILEEQSQELEGVHSHEDENPYEASSPTLANAKSKAPNSSAHSNIDLYLPDSRILRDKLRSARNYVPNSQKFKKMTGISSFIKINEKYSLFDRYKSSYGTTSTNATELFGNSVTEDLVAPQSKSALSLKVTQSIYKLVLEALTRSIELMPSQISLHSVELFKLMLYKVGPSYIAVGLESLYDTYVDSQLKNKSVFGRGATTDIDLSFLSQFYTIFVQLYLLSTVVKKSFYPLVSSEHDTNFISHSFNLFLQDVEIGVNVTVNDLVGIVQERINLILSKQPVNDYMVVSESDRTPTCDSVVAFLENILNSALEELSFDPVLKLRFVNKISSYFLSMLILHLSHLKVSMNGFTLLTHDLAQYILIFNSIKIDNTESLDLYADEEGSRNAKGDNDNKWVKEQLDQIQSAFKILNELPGLYTCQPESLKEFCSEGKLNELKKSVIRGYISNREDFQTWFLSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKH3</accession>
    <name>A0A1E3NKH3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16442</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017699.1">
        <property type="nucleotide sequence ID" value="XM_019161206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKH3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKH3"/>
    <dbReference type="GeneID" id="30177893"/>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF160">
        <property type="entry name" value="ERAD-ASSOCIATED E3 UBIQUITIN-PROTEIN LIGASE COMPONENT HRD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="243"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="420" mass="47791" checksum="CCAECBCA235F43A7" modified="2017-01-18" version="1">MKTRIGLTHLVRRYYSSGAASTTGNPLLLDILPSKKIISRLLFDYDARYNFSKYLPVVESIYDSLPDVFGKEPEIPERFRGNDLLIFQRVLAQIRKQTHTINPLLLKLENELVEHAAERGSRDALCTLSFMALDNNGEGWTEDDKSTAKKYIKDLMEMEHPLAFKLAADRELKGFTESLNQNEEEEDMSVDAKVLRTEESTADLSPTSDTSFTNITSSPKRLARAVSLYDHFLKLDPKSTVAAAAHRSLGMIFFRTQELVKAAEHFKHAVQLAPASDNAQAHFFLGLLNEIDTLKARYHFQMAAKEGFRESFANLGYIELNVFNELKKAKEWFALGAELGVPECIVGLFDVAYRGNDWKRAKDIITRAEKQGVGKMLYDSRKDNVEKVQEITSFDPIVDEQHAEETGDNNNQKRRSRWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKN1</accession>
    <name>A0A1E3NKN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_16516</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017793.1">
        <property type="nucleotide sequence ID" value="XM_019161235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKN1"/>
    <dbReference type="GeneID" id="30177922"/>
    <dbReference type="OrthoDB" id="1045173at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="93"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="522" mass="57099" checksum="362EFEC1FFD6EAA8" modified="2017-01-18" version="1">MPAELQDAVPDIVIGGDQPYDDVQYESENEAEANEDVKTEKKVSFAELDEESGETEEAIRQREFEEGGGLPEQTANPDITTLTPISPEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKNELERNITIKLGYANAKIYKCDNPECPEPDCYRSYKSDKEIHPKCQREGCSGRYNLIRHVSFVDCPGHDILMSTMLSGAAVMDAALLLIAGNEPCPQPQTSEHLAAIEIMKLKHVIILQNKVDLMKKEAAIEHEKSILKFIKGTIADGAPIIPISAQLKYNIDAVNMCMAKSIPIPMRDFSAQPRLMVIRSFDVNKPGAEIQDLKGGVAGGSILTGVFRIGDEIEIRPGIVTKDDNGKIQCKSIFSSIISLFAENNDLKFAVPGGLIGVGTKVDPTLCRADRLVGQVVGSKGNLPSVFTDIEVNYFLLRRLLGVKTDDQKKVKVRNLEVDDVLMVNIGSTATGARVVAVKADMARLTLTSPACTEINEKIALSRRIDKHFRLIGWATIKKGTTIDPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKN2</accession>
    <name>A0A1E3NKN2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_133368</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017759.1">
        <property type="nucleotide sequence ID" value="XM_019160046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKN2"/>
    <dbReference type="GeneID" id="30176733"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="129" mass="13665" checksum="507B1061B4110854" modified="2017-01-18" version="1">MASLPTPDPHPACYPRLPFAATPLSPPSSRLLRFFCPSCCLLLPSLTAASLPASLSALLSAQSRPPKASPRGLRGILTDLLRIRVRQFAIAGVRHCSVLASFLRLSVSAYICPATGRDILGRAGACRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKP6</accession>
    <name>A0A1E3NKP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_28055</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017838.1">
        <property type="nucleotide sequence ID" value="XM_019162530.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKP6"/>
    <dbReference type="GeneID" id="30179217"/>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1047"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="503"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="523"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1362"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1395" mass="157707" checksum="B720F46E0A8EEAF6" modified="2017-01-18" version="1" fragment="single">RISDLIDAMSTHKDPYLVMETLNEISNSLLMMTSIQSERQVPTYLLAEKLVAVISGYPDHLELQVAACRCIYNLAEVNYELMHEIVSAGVIECLNSKLLDLSYIDMAEQALQALEIISRKAGKQCLNKGSVPIVLTFLDFFTIHTQRKALQVAANASVFIPKTKYNDIIDVFPTIKNVATQYTDAQCVESAWIFIANTIRNIYTTPEVLTAMIDLEFIKKLFEIFPLCLGNGTKSSGLVTFKTCILLLDSLSLLTSYSPEFSNNLLQECDIGSMIMQIFLSYEHDDVSHSKNSTVTIDALLKCPKELLVTLLKLISSIIPLNSTAIKSQQKTDIGNYTLVPEEKHNINAEKIILIKSNSESLHDFNLLIFPLLLNIYDATVEFKVRRLVLLSVLRMIYMSSPDELKSIIFKSKITSILASTINRGKNLLQHTIKNSKNSEDFKHYSLIFGSLLIIQSLINGNPVTFLESFTKEGLIAQIQEFTLATTEEYNKIVDTSETIGEEAEGFSNSSGSNADHAGSEAVNEDEDGDGDYENWVEDDNNDDETGTSADDAETFGESSSVLHHFKSINQSFSILDDGLKLLSLRTILESLLSLSSSINFDYETLSVSNNFTKSESMKFLETFISTLRGDLTLVSENEWIDLWTKFATILDSSSNNKSTSSFELVSSGIINELLHTFQGRKNFESPVCKTFLYIFCSALSPCVNEKTSPLFFLVKKLEESLDRNESFDIVGSDSDASQSSRFRAASMTKQMRIKLVPMDSESSDKNKSVLLVVQAIATFESIQNFIKNSKSINLGNNFNLNSSSLDDDYHYEFFIDNERIPSDATIFGVIYKSSENQRNLNDLKGDLLKQAHVIKYKYVPGKLIPDDSSAFEETVDDALTNIGEKSTIDTLQLLKILYTMNQNVQNPSTSEVLFLNYKLSAKLNRQLDEPLLVASGILPDWAIIIPREFPFLFPLETRLFFLKSTSFGYSRLIDFWSSKSKEEDSNSTTRNPMLGRSIRHKLRISRDRIFSSAVKVMENYATNPGLIEIEYFGEVGSGLGPTLEFYSNVSKEFGRLKLRMWRSDQYSKLQYNNELYVHDDHGLFPRPLHKLDPHLNNTLLYFKVLGKFLARSFLDSRIVDFHFNPLFFEIAMKYSATKSFVHDISDSIKKIKSVDQSLSSSLQHLKLYLDEFARIPEADWEEVQISGCKVTDLMLTFVLPGYEEIKLIPDGDEIGVNSSNLGMYINKIIDFTIYGGIENQIKSFVSGFSEIFPFTSMILFSAEELTRLSGNDVENWNVETLITMIHADHGYNNKSSQVEWLIQIMSNFDKDERRKFLKFITGSPRLPFEGFKGLSPPFTVVLKHCEDKLQPDDYLPSVMTCANYLKLPCYTSKEVMYTKIVQAMNEGNDSFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKR3</accession>
    <name>A0A1E3NKR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017823.1">
        <property type="nucleotide sequence ID" value="XM_019161251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKR3"/>
    <dbReference type="GeneID" id="30177938"/>
    <dbReference type="OrthoDB" id="2141050at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39218">
        <property type="entry name" value="OXIDOREDUCTASE 14 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39218:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 14 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="71" mass="7977" checksum="BF635CB6D0227CF0" modified="2017-01-18" version="1">MLLAWTAFGVGVRALQMGIRQAPLLHAPMGFVYSAAFTTTVGYYFESWVEKNDELLELRLAKLKKLREASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKR8</accession>
    <name>A0A1E3NKR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017036.1">
        <property type="nucleotide sequence ID" value="XM_019164470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKR8"/>
    <dbReference type="GeneID" id="30181157"/>
    <dbReference type="OrthoDB" id="10250730at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07729">
        <property type="entry name" value="AHL_lactonase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF2">
        <property type="entry name" value="102 KBASES UNSTABLE REGION: FROM 1 TO 119443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="66"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="715" mass="80428" checksum="1E5E3EB9E5AEBB5C" modified="2017-01-18" version="1">MSREYAEGPGSIIKTQVANVAPEGTKVFILKLGNLEADASFFFEGGNVQLKSDKGIPKVHERRNVQMYTVLIDHPISGLILYEVGPGKPGWEEKWGSEVLDAYCRVNKDDETQALDVAIRNAGYDIKDVRHIIVGHLHIDHAGGLEYFKGRQDVIVWVHEIELKHAVWGIATKSEIGSYLKYYIDLDLQWNTFNEEKVDLFSGLTTHLMPGHTPGLIILQVNLPHTGTMIFTSDHTIFREHYENGGRKQGFLMRDHYQWYNSTQKLRRLQRDTNAQVVFGHDPSIVEELLATEPFFMESAMSRQQDSQTLQGFLAQKGPAMDAWFKRALEDESVAVKKPRALTTVEGRSGVRRISIRNHQILADSPPEWVGFNLGPSAPEILLGALGACLTHMYLVVAGIKKLPINKLEIETSGEVDLRKGAEGHEDEIPGIDNIHYVVYIDSELTDGQLDHLRTDVEKVCPLYNLVKNPATITSSYVRTTRVAFVRGYASTAEPVATAATEVRNTQKAADSRKTYLIDMYTHMWRENSIVLLAHHNNLLSTENENIRKQLKKIDEKNKTNKVEFRKLKGSLFKYFLRASSHSDPASKAAGRMIRRNKVRHPLENLLKGPTAAILIKDLDPKTVKDVSKVLATQKERLFVMGGKVGDEYMTLEDIETFKNLQSLPELRAELVGLLTMASGAGIVRTLESTTNSLALTLESRKNELDKAENPEQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKU3</accession>
    <name>A0A1E3NKU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017885.1">
        <property type="nucleotide sequence ID" value="XM_019164299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKU3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKU3"/>
    <dbReference type="GeneID" id="30180986"/>
    <dbReference type="OrthoDB" id="3998016at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="586" mass="66486" checksum="2848992C5D397AF8" modified="2017-01-18" version="1">MPVATKRKRSRKTTFKTNPYPVQETKTIQQSTSNPFTFGPDLKTSSFSNRQNITNGGNPVFQFHIPNVDPHKATLLNDEVDMYFPRKHFLSKYQMLNDLLENIIIKPIPTDKIIPPRLFPIAFVDGMPYEKKRAELLKDVSEIAKHKSQDDLVDLKNDEKSQDANLTLDQTKNEIKTEIEDNKEAAPKLNSGVVTDFNTTQERKKEENKTEVSKDGKNNSKNDSGKYDVNLKNEDGTRAFVKDIEDDLSDYDPDFDGYQIPYPKDSTETKKVINDYLKSRMKIKVRTDFLFGDLETMKMQEYSLAEIEKETQQKGQEPFEFGERFNYNMKAIDDLHQTFLSYTANSIEECENKVSEIESDLKSKFKKRFVDSNEKNQFKTQAFDSFKTGLTVEDYNSQFKKVSLQANDIPQNLSSALSLQTNASSIVRGENSDVKIDRNPGASPTSQILIEPPASISFSPRDLVATNEEATLVSTSQNQEQNISPSLPLAHGDTAVADQISGQGSMDLVPQDPSEINDHVSDVEKGSQDLVTDLTKKTSNNDMNNFLKSQNNFDFENEMLYDSADFNNDDNDDDFGSLSNEVFLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKV3</accession>
    <name>A0A1E3NKV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="5">3.2.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Endo-1,3-beta-glucanase btgC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Laminarinase btgC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_58996</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation. Active on laminarin and lichenan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="5"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017880.1">
        <property type="nucleotide sequence ID" value="XM_019163335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKV3"/>
    <dbReference type="GeneID" id="30180022"/>
    <dbReference type="OrthoDB" id="68336at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF17">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BTGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ46767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="321" mass="37244" checksum="95828EDE4384A788" modified="2017-01-18" version="1">MMETDDLKFMFSGIAYAPEGVMEPECGATLRNVILDIARLSKITGSVKTYGTQCRQAEFILEAIDQLQLNFTLSMGVWIGSDEATNQAQLHVMKDLIKRYPRKYFRSIFIGNEVIYRKEKTVPELMGYVREAKNYLLSLNITDLPVGVSEIGSQITKEMLLDCDFVGANVHPFFGGVDAQFGTRWVLDYYHNQLLPLKESINTTTEIIISEVGWPYQGGEFIRSVAGSWEYQQFLNDWLCTSPANILNNCFYFEAFDEPWKRIWWDNDRTWETEWGFFTSDRKMKKHIYIPDCSKYGNPSLVDFEYIEEVEDPYANDEDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL01</accession>
    <name>A0A1E3NL01_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017927.1">
        <property type="nucleotide sequence ID" value="XM_019161288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL01"/>
    <dbReference type="GeneID" id="30177975"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="68" mass="7584" checksum="3419172847E3FBEF" modified="2017-01-18" version="1">MQLPLPVRTFVVSAPSWAAKPKPISMTFLTQRKSRGPVGPVPVSCLIRPGLLNHNRSRSRNRYTFPIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL77</accession>
    <name>A0A1E3NL77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017206.1">
        <property type="nucleotide sequence ID" value="XM_019161543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL77"/>
    <dbReference type="STRING" id="763406.A0A1E3NL77"/>
    <dbReference type="GeneID" id="30178230"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="636" mass="70579" checksum="75EC560B04DFDD78" modified="2017-01-18" version="1">MSVASSDQDTRERPKKTAYRAVTRLPKTRTKTGCFTCRKRKKKCDEKGPVCLGCSRNFLKCVWPSYATETLPKNFTISSCTVDDFESGVPAVASRQAEMETGITAAETASPTSLGDDGPFDEDIPNIPSSLCSVMEVRSYKHKVFYPHTTTIPESIDFVIESISGSGRHRRRTASGVSACESDSTSSSSQFVPSLDDIGVDHHAISQMFSSLYSKSVDSDLPISSSDSDENFYEGLLNYSSKANIPAVSIKNPVIAAFREIFFARGCSYLAKSNLDENPEVSEKYTAAANRHYDNSVVIMSNHAVSYGAKTKTSTGVEHWMVFSMRTLCMADRLMGLISENCISNLISVEGRISSVEANSTLDGLTDCAARNFDKVLFSEVLFCYPFLIYFSKFDSLLEIVPPVKLFQNYNEEMVAIFLDSSSQPAPNLEVNEGSSGWLRNVLNTAVINVLQNLTKMMWLLRMRNSLETDQLNTSLKHLKTDISLIWTTIQTAEIQLDKEDPFVDFAKLSHMSSEILFLAVSDTPINASSPIIGFYLDQFMASYESYLKNSNTHGDTIAKCFFVLPLFVAACAAQTLYQKEFISKELYILSRDLGHDFVEQLMLSVEDAWCAEQNGDATTFSKLVSRDGFASLARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLE2</accession>
    <name>A0A1E3NLE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_11860</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018009.1">
        <property type="nucleotide sequence ID" value="XM_019159850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLE2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLE2"/>
    <dbReference type="GeneID" id="30176537"/>
    <dbReference type="OrthoDB" id="6093671at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002127">
        <property type="term" value="P:tRNA wobble base cytosine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057285">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057286">
        <property type="entry name" value="PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023270">
        <property type="entry name" value="RCMT_NCL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808">
        <property type="entry name" value="NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808:SF1">
        <property type="entry name" value="RNA CYTOSINE-C(5)-METHYLTRANSFERASE NSUN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25376">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25378">
        <property type="entry name" value="PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02011">
        <property type="entry name" value="RCMTNCL1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="12">
        <location>
            <begin position="51"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="415"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="662"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="415"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="662"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="168"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="695" mass="79260" checksum="4B8B19D08C624466" modified="2017-01-18" version="1">MGKKGRRGRGGNKLGKRDDRQSWDQVTQENEAWEKYYRALDLIPNDQFDLFKSTCKETLPLTFRVTGSRKHAKIIANLFQEKIMPLLENVEGTESYNKPFSLPWYPDNLAFQIDLPKQVIKKLPQYAAVQKYLLVENEVGNISRQEAVSMIPPLVLDVEPHHHVLDMCAAPGSKTAQLIESLHTFKDSNDETLKPTGFVMANDSDYRRSYMLVHQIKRLNSSNVIVVNHDAQMFPKMRLEENGPYVKFDRILCDVPCSGDATMRKNIMVWKEWRFGNGIGLHPLQLNILKRGLQLLKPGGRLVYSTCSLNPIENEAVVASALKLWDGKVKLVDSSDRLPGLIRHEGISSWRVFGKDGEEKQQPLEDDKLPATVFPPSDEEAKDLHLERCMRVYPQDQNTGGFFITVFEKEAEQEVEQEKEVTNAENKRGASETVESETTKKQKTEESEVTTVVKQRREKLPIDANEEPFIFIEKGNAELQKCWDFYEVSDDFSKDCTLVRNATGEPLRSIYYVAPIIKKILQLNEQKLRFIYTGIKLFVSQKSAVCPWRIQNECLNVIRPYIPASRQLETNLELLKLLLLEASPSVAEIRNSNADPKFMDDVALLGEGSCFVKIKTDKDGNEELFFPLWKGKSSFNLMVNKHETAELLYRLYGIDKAALDKKKAEENAAEEKEAKEAADADTNATESEAKSEEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLL5</accession>
    <name>A0A1E3NLL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial ornithine carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71156</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018142.1">
        <property type="nucleotide sequence ID" value="XM_019163847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLL5"/>
    <dbReference type="STRING" id="763406.A0A1E3NLL5"/>
    <dbReference type="GeneID" id="30180534"/>
    <dbReference type="OrthoDB" id="2139348at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF31">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="9"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="111"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="213"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="33671" checksum="4DCDCBE5D52E96F2" modified="2017-01-18" version="1">MPNDKSVFSNSLHEILLGSVSGACGKVVEFPFDTIKVRLQYSQSLPQPLFTSTFDAIRKTYHNEGIARGFYKGLRVPMMGASMEVSCLFFSYRLAQDLVKLSKGKALTDELKVSEKLICGAFSGICTSFILTPIELVKCKFQVENLKNLSNPNFVSKTIPQITRELYKSQGIRGLWRGHLATMIRECGGSMTWFGNYEFVIHYFANGEKNYKPKVHELMVAGACAGIGYHCTLFPADTVKSILQTNDGGNLTFMKVVKHIYRTKGIFGFYSGIGVTLFKSIPTSSIMFISYEKLKEWLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLQ2</accession>
    <name>A0A1E3NLQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="6">HMG-CoA synthase</shortName>
            <ecNumber evidence="6">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_71176</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="6"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018163.1">
        <property type="nucleotide sequence ID" value="XM_019163851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLQ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NLQ2"/>
    <dbReference type="GeneID" id="30180538"/>
    <dbReference type="OrthoDB" id="1269963at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="8">
        <location>
            <begin position="174"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00073381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="446" mass="49418" checksum="4F7960A4C915936A" modified="2017-01-18" version="1">MTVQNVGIKAIQFYVPGQYVNQTELETFDGVSAGKYTIGLGQTNMAFVNDREDIYSMSLTVLKNLIDNYNIDVNSIGRLEVGTETLLDKSKSVKSVLMQLFGENTDVEGIDNVNACYGGTSAVINCVNWVQSDSWDGRDAIVVCGDIAIYEKGAARPTGGAGTVAILIGPDAPIVFDPVRGSYMEHAYDFYKPDFTSEYPVVDGHFSLSCYVKAVDFCYKAYSKKAIAKGLSNSSTETVGIDYFDFNAFHVPTCKLVAKSYARLLYNDYKQDPSKFPDIDSTKLSSLDYETSLVDKTVEKTFMPLSKERFNKTVKSSLELPTNVGNMYTGSVWGSLCSLLSIIGSEELQNKRIGLFSYGSGLAATMLSVKVVSDISPIVSKLDVKQRLDSREHKSPTEYEAAIALREKAHLQKSFKPEGSTDYIPKGSFYLVEIDDKYRRFYEIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM10</accession>
    <name>A0A1E3NM10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">ATP synthase subunit d, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_11387</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ATPase d subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017496.1">
        <property type="nucleotide sequence ID" value="XM_019159783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM10"/>
    <dbReference type="STRING" id="763406.A0A1E3NM10"/>
    <dbReference type="GeneID" id="30176470"/>
    <dbReference type="OrthoDB" id="35799at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008689">
        <property type="entry name" value="ATP_synth_F0_dsu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036228">
        <property type="entry name" value="ATP_synth_F0_dsu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12700">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05873">
        <property type="entry name" value="Mt_ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005514">
        <property type="entry name" value="ATPase_F0_D_mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161065">
        <property type="entry name" value="ATP synthase D chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="173" mass="19064" checksum="535EC37048F943AC" modified="2017-01-18" version="1">MSVAKSAATKIDWSKIIPQLGLTGQTASSLTAFKKRNDEAKSALYVLKSQPTEVAFDNYSKLKNTEIVSKIKSDVSSFTASTIDLSKQLNVIKAFEVKAIENAKKTESLVLAELANLEKTLDNIESARPFEQLTVDDVVKARPDVEDKVSDMVSKGRFEVPGYKEKFGDLVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM23</accession>
    <name>A0A1E3NM23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_57740</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. This subunit is a non-integral membrane component of the membrane pore domain and is required for proper assembly of the V0 sector. Might be involved in the regulated assembly of V1 subunits onto the membrane sector or alternatively may prevent the passage of protons through V0 pores.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018305.1">
        <property type="nucleotide sequence ID" value="XM_019163256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM23"/>
    <dbReference type="STRING" id="763406.A0A1E3NM23"/>
    <dbReference type="GeneID" id="30179943"/>
    <dbReference type="OrthoDB" id="10250083at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000003">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018497">
        <property type="entry name" value="V-ATP_synth_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR018497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="352" mass="40492" checksum="61EE2611A39EEB0D" modified="2017-01-18" version="1">MEGLFFNIDGGYIEGLVRGYKNSLITSSQYINLTQCDNLEDLKLQLSATDYGNFLADISQTLSTSVIEQKLNKKLIEDFNYIKSQATYPLSKFMEYITYSYMIDNILLIITGTIHNRDKGEILKKCNPLGWFDTLPTLSITTDLNSLYETVLIDTPLAKYFHNCLSIDDLDDLNIEIIRNILYKEYLEDFYRFCVAELPNPTDEIMERLLSFEADKRTINICINSLDTDLSSDEKLKMLPSIGLLASNDAIKYQLSELADLENLKILMSSVGPYKDFFSDSASAGVSSKSLEDSFYEYEMNLNRNAFTQQFTYSTIWAFIKSKEQEIRNITWISECIAQNQKDKINNYISVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM82</accession>
    <name>A0A1E3NM82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018362.1">
        <property type="nucleotide sequence ID" value="XM_019160728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM82"/>
    <dbReference type="GeneID" id="30177415"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="51" mass="5658" checksum="87E6E7C34FE49779" modified="2017-01-18" version="1">MGVRAMGKRKKGKKEEKTFPRQCKGLAGVRDMLQAEGKNKKTTDAPQPLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMH9</accession>
    <name>A0A1E3NMH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_111269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018440.1">
        <property type="nucleotide sequence ID" value="XM_019159757.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NMH9"/>
    <dbReference type="GeneID" id="30176444"/>
    <dbReference type="OrthoDB" id="3998151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="646"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1139"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1191"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="758"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1147"/>
            <end position="1158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1375" mass="152269" checksum="25F646E0D28C667E" modified="2017-01-18" version="1">MADKEGSLSHANDAARDAYQIGLNYESLPSPFLGNGISSGNGNGNGSGIRNGDFTYNPGAVGNGVNSGNALDANRPQLSQHGSISATDMSLSNTNHSNLSGTRYPVQQQQQQQQYSTGIQYRGRVVHIPPEPGYLGPPTAGVYNKAYSMGDSQVPVSWVTHNYNQNIVQAPPYISAASPSPSPPDSASHIVSGNDNANQTEINPLKNGRRPGNIRNSEAGLNAELHENPPVFDFIKQASHTHSNQGNGNMTNMARGVMNSQEGELPSQTRNNTFTVSTAAPSSAVATLPSTTGNANFQFSNPNSISSGLPVYHHQHHPHYQASQELNRPYYQEFQSAHPNHRPSNISLNHSQQHMHHHPAPSQPQANHHIHQLKNQLIPAFNSPSTSLENSIKSSISSHNVRMQPIQQQSQIHVSKQQMLLQEAQPFVQQHSNPTQTHNYKQNHNLSSTGRKIIKSPATQISNIINHDVPAVSPINSTRNSRSSISSLLSDPIPEKIDNPSSKTPRPLKAEKIAVMADDLHKIGEADESEKVTELESAELDKSIKLNSNLISNDKALPDSKARDGNETPVNAVVHLDPFITSSPADKFITALPVDESYDTSLNESVIQANSVATTKTKCESKVTQKIPAKRGRKPKKVKTQVLVVSDRNKGNKSENETEAAPEKKKDGVTDPIKVTEKEEAKVDARGNEKDRQLFKKTASTSKFITLKINSNKLRNILDAATESTLTSLPPKSGDSELLVLIEERSPKEGNTINNETIEMTNQQLVQLQQNNRQLVSHLDDIKKALPDLFQLNAESIPHLPKGYVPLIMAPNGLLIPMEYISVDPRERKVSIMRPLWEATSGATDADSLANGISTNAIDENVEEIEGSTRNGRKDFGFDNADKLASLQLYLFSKEAKMNMNKVEKVRGCKRFRKQISYYARLLEREQMGFIENYRQERNKCQKMLNRLESGEEMTDTEKDLLEKKDYEMLREEIAKNYNDEHNYLSYLDHVIELESIQCLDYAARLVKLKNYLNMERVRLERHKNRLCRINTNKSHSIWKRYVKTGGNRGRRANVESNSSCGNNSNSNTNWNVNLNLISQNDFMLLTNPNSRAYGTYVLNNSMDKGNENQSEIVELVEYLLPEKSTLRELYREMKRLESDNHDRNAKNTSVNSVQKNDMISKYGLKESNSSTGNRLLRELQIDGMNVDFHKNDRVERRGSGQRKARNSVSSWRHGGHGGHGATRNAHAGSTDLSGVASENPDLHDGEDTPDSCQGDYSLRNAAAGINMLRERSASRSDEQDSAEQEAGAYGGLVDLAAAAGSPPPTVTGTGTGTRNVLNITSCLDPTLRDVDRVKMLNLNTEEIGLSFTRVYGMPKGLRPDEVDADLAYLRSALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMI4</accession>
    <name>A0A1E3NMI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_71420</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPase-activating proteins (GAPs), thereby resolving their association.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Fungal signal recognition particle consists of a 7S RNA molecule (scR1) and at least six protein subunits: srp72, srp68, srp54, sec65, srp21 and srp14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The M domain binds the 7SL RNA and the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit srp101. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018453.1">
        <property type="nucleotide sequence ID" value="XM_019163918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMI4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMI4"/>
    <dbReference type="GeneID" id="30180605"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.140:FF:000001">
        <property type="entry name" value="Signal recognition particle GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="14">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="528" mass="58134" checksum="F788CF45803D6C63" modified="2017-01-18" version="1">MVLADLGRRINNAVSEVTRSNVVDQSVVDTMLKEISNALMEADVDIKLVIQLRKKLKGKIEAEEEKPGVNKKKIIQKAVFDELCDLVDVKEAEVFKPKKKQTNVIMFVGLQGAGKTTTCTKLAVYYQRRGFKVGMVCADTFRAGAFDQLKQNATKARIPYFGSYTESDPVKVAHEGVEKFKKEKFEIIIVDTSGRHRQEEELFKEMVEIGEAVKPNQTIMVLDASIGQAAEAQARAFKESSNFGAIILTKMDGHAKGGGAISAVAASKTPIVFIGTGEHIHDLESFTAKQFVSKLLGIGDIQGLMEHVKTLNLDQNDSIKNFQEGKFTLRDLQSQMNNILKMGPLSKIAQMLPGGIGDMMNQVGEEETSKRFKRMICIMDSMTKTELDSDGSLFIDEPSRIIRVARGSGTSVTEVEAVLLQRNMFARMAQTTKNMQQNMPPGMGKNMNPQQMQKAMQQMQSNPGMMENIQKMMGGMGGMPGMPGLPGMGGGAGAGGMPGMPSQQDMMKMMNDPNIRQMAQQMRKQFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN78</accession>
    <name>A0A1E3NN78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71620</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018675.1">
        <property type="nucleotide sequence ID" value="XM_019163966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN78"/>
    <dbReference type="GeneID" id="30180653"/>
    <dbReference type="OrthoDB" id="5121955at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF40">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="611" mass="71347" checksum="0ED271222890B3D9" modified="2017-01-18" version="1">MTKISRKRPISSCKYCYKHKVKCDRGNPCGNCIRLNNSSCEYNFQSFLHHDLKNQKSTEKRNSSIVTYPSNALFPHLGSMMRKFIMNSISNKTASCIGFFDFSKEADSEYFRVHIHELLPEKKTLTKCFSMYWNCIHPLLPILDESAICKKFESFLKENNLAEKEEVYEPQFLIVIFAVLFSTTIQLELIISDSEKRKELQCQKDKCYVGFKRLLALLNFPNVPSFECVSSSLIVYEVGTFCYRQIAFEICVLCKTLCSMGIHLATNNNKATMQGITLRKQVMWHTAHKIETMSTIWCGSLPNANLHVDIVGFPNASGSGAQSSLSVKTVNPFIASQNLIYKVLPYFSDLTIFLNVDTESDYYRSFDFTIFERKLFSLCQHFRDVQDEIVRCDTTKFEKKTNRWLIANSRNMLYRLYFLYYVCLEDKTTRSRSSSVLQNSKEIENIKNKLYKADENLYTNFLKMRKPYDGKLVEIYLVILVKDIYNQPHKRYTFEDLNESVQLLVEEKIFSADGDLRVWLVSEVLKRLDSFKKFWPESSYVIYDFLVNLWNFVESPRQTLSDETFELLLNSSSLKEANGSPLSSIIDYLPFEFANNSVEEYYHYFDFPTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNC8</accession>
    <name>A0A1E3NNC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018722.1">
        <property type="nucleotide sequence ID" value="XM_019160868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNC8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNC8"/>
    <dbReference type="GeneID" id="30177555"/>
    <dbReference type="OrthoDB" id="4089008at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007147">
        <property type="entry name" value="TF_Vhr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04001">
        <property type="entry name" value="Vhr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="736" mass="82736" checksum="88EE825BDD0812E6" modified="2017-01-18" version="1">MSSEVVSSTLTQDDSLTRKKSTTNSNNGGENHGSRSSSSSNGTTSIIRKKLNFTDEALWKQFSSRRLQLVESLSLSSKKASEQDEEIKVCANTLMTEFHFAESSLPEFDKLVRLAIQSVRRNKKRSEKRLASKLETFTSDDEQFPHSIKRPKAERHGSGAGSRSDERSDENDDGAHNLNLLSTILIEEENHNRLHSHSSDYNGENDGIPNTDSEITTSNSIVTNPKKTSSVMDFSLINHNHHKSGDHKDTENIDSRVAIDSLVAPIIQDSDKLPSIKKLDESPEFANESQKILHKIKNSKSCFEFSKNSGNDYSRTQNYDLLEEFGSSCINAAVLYTLEKWFDHLLPDSSSYIKLRLKSDLTLGLIIKNLDTESVEVNRLSNYVASQLFKKLIGGCVKDYGFDSTLNPLCDIFHSIILKDYPIIAKDQLRKNQDLDVNRNYAKNVHSSKEVNFRESNTTEPKTNYFKNLSVIDSTISQPSAHSVDLFTTSPVNSSANSKTHDSDSALIEHKMNFLSHSEMPDFPLSTAHIPIPPANIPYLQDHQMMDSQMKSSTIAPPPLLRSKQYTNVTIKFKEKDLKFRYCMDSNAPPTVLELITNCKQAFGVVTSTRVLNLRNLRSGDIIKTDHELERVLRMAAMLSTDGNGEVGLELVFANVTQGYLNLNYDSSTKSKNKNEREHPAGGDDDRKLPAEHEHEHHLNSISTGDSLIGRYLPPPRPIRPDSQPRNTFMKFQPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNH8</accession>
    <name>A0A1E3NNH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of mRNA-decapping protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_10632</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018770.1">
        <property type="nucleotide sequence ID" value="XM_019159701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNH8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNH8"/>
    <dbReference type="GeneID" id="30176388"/>
    <dbReference type="OrthoDB" id="10030313at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612:SF0">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFDF" evidence="7">
        <location>
            <begin position="174"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal" evidence="6">
        <location>
            <begin position="329"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="89"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="589" mass="65553" checksum="817FB55845953B4D" modified="2017-01-18" version="1">MSQFYGYTVDLVFKEPAGKTVRGIIERIVENEIVLKNPALVLPDGSYEKVNKKELEIQTQEILDLNVVELAKSNSKYIKKQMKLLKGKEKSKDVASNDSKDSKDSKDSKEGRDVVPTGKKTKKYTDISNASTNGSFSSDSELIAASSSGSSSGASAKKELDVKYVDIYNEKPTITKPKKVSQLEEFDFASNLQKFDKESVFKNISKHDKVDQSSRLVSFNKVEHMDKYGNDEMILTQKQNNDWDNNKAFDYSNIVKPLANDHVTKYNETSPSDAGENTSLSSSYLNSLQGRHASNTSFERKASMTPTFAQFFSSVYDDSPIPTCSTLQLSSVLSACKEKFGLTNQILNENAGTSISELIITNIIGSFRIGFKNHNEPPTVLLLVGNNRSGSIAINTGRHLFNRGLKTIVFLLYDKEHSEEDLLAEVDDELKRYSDIGGKIVNSVPQLEKILKTVESPLEFALDGLQGFDSDINDLLETELDQVIQLIEWCNESGLPMMSLDIPTGLSASSGTNENDNTLLIKSKYLVSVGLPLSSALNMYKFGYFEKGNITHYLADCGIPRRVFNSKASLRKFDRRWFADSGTIDLKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNS9</accession>
    <name>A0A1E3NNS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15181</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018306.1">
        <property type="nucleotide sequence ID" value="XM_019160702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNS9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNS9"/>
    <dbReference type="GeneID" id="30177389"/>
    <dbReference type="OrthoDB" id="1661054at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="418" mass="46598" checksum="A28F0EB65DA93BE7" modified="2017-01-18" version="1">MASHTQLLLDTLSVNLPAGSQELLAEPAWQDTALQYLTELLDADDVETLLDTATPLPGSVKGGGGGGVGIAEQIAELANESVLLDKKIFGRLNSSATSNLALVLHTNTVFHESFENFDSLVLLAQELVELDTMAGWKTQVAAVNSSLGLAAGGSGSARPGPHDDIVSATYSTELSSILQNLQSLTEILELPAITHSLIKLGQYSECIEISSLSKRLRIRYNDIDLVARIEENITYEINDMVRKLSSLLMTNLKQASMIKILSYLKRIIGNRDQLKSIFFHSRYKFITDEFDTLLPLKQSRLIEKYLKRTLEIFREFCFQTVITFDSVFDRDRHLTYVFLRSLTDRLCAILVENLPLLAEDSACDSLLLQLIYCCQSLSRVGGDFTASVLRQLQPAVDQRRLLTIVRKQKELVRSLNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNW7</accession>
    <name>A0A1E3NNW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase</fullName>
            <shortName evidence="13">HMP-P synthase</shortName>
            <shortName evidence="13">Hydroxymethylpyrimidine phosphate synthase</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_32687</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-(pyridoxal phosphate)-L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + L-histidyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 2 Fe(3+) + 4 H2O = L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + (2S)-2-amino-5-hydroxy-4-oxopentanoyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 3-oxopropanoate + 2 Fe(2+) + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:65756"/>
            <dbReference type="Rhea" id="RHEA-COMP:16892"/>
            <dbReference type="Rhea" id="RHEA-COMP:16893"/>
            <dbReference type="Rhea" id="RHEA-COMP:16894"/>
            <dbReference type="Rhea" id="RHEA-COMP:16895"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:143915"/>
            <dbReference type="ChEBI" id="CHEBI:157692"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:65757"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the NMT1/THI5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018897.1">
        <property type="nucleotide sequence ID" value="XM_019162783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNW7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNW7"/>
    <dbReference type="GeneID" id="30179470"/>
    <dbReference type="OrthoDB" id="434407at2759"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106344">
        <property type="term" value="F:4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13650">
        <property type="entry name" value="PBP2_THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000187">
        <property type="entry name" value="4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027939">
        <property type="entry name" value="NMT1/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528">
        <property type="entry name" value="4-AMINO-5-HYDROXYMETHYL-2-METHYLPYRIMIDINE PHOSPHATE SYNTHASE THI11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528:SF1">
        <property type="entry name" value="4-AMINO-5-HYDROXYMETHYL-2-METHYLPYRIMIDINE PHOSPHATE SYNTHASE THI11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SsuA/THI5-like" evidence="14">
        <location>
            <begin position="16"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ47784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="340" mass="38217" checksum="67E77A2D1BFE03BD" modified="2017-01-18" version="1">MSTQKITFCLNWQAAPYHIPVYLAKTKNYFADEGLDIAILEPSNPSDVTELIGSGKVDMGLKAMIHTLAAKARGFPVTSVASLLDEPFTGVLYLKSSGITNDFQSIKGKKIGYVGEFGKIQLDELTKYHGLTKEDYTAVRCGMNVAKYIIEGKIDGGIGIECMQQVELEQYCKDAGLPESDVQMMRIDRVACLGCCCFCSICYICNDEFLSANPEKIKAFLRAIKRATNDVISNPEQSWKEYVDFKPQLNNDLSYKQYQRCFAYFSASLFNVHRDWNKVTNYGKRLDILPADYVPNYTNEYVPGSEPKEVDDPLEAQRLMAIHQENCKLNGGFLRLAINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNX6</accession>
    <name>A0A1E3NNX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_71809</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018890.1">
        <property type="nucleotide sequence ID" value="XM_019164014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNX6"/>
    <dbReference type="GeneID" id="30180701"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="89"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="336" mass="38404" checksum="BF931226AA5D1F3F" modified="2017-01-18" version="1">MCNAFYAAKVCHEQVQTFLASSPTNSLYSLKSTFDNDNNPVSNLHNKLHLPTLYPPSFNATLHQSAQNQVLRSYNDFLSVLNHWNLEQLSISYNGGKDCLVQLIIYLAAIYQFVEVEGKGSEREPHTIHVNAVYVHTENEFEDQLRFLEKSVLQYGLDFTAVYTTIDANDYDSSQILTGKANELSKGKITCLFSPTPTLPCGFLTYLKSNESVEAIVVGIRRTDPYGASLKLEQRTDTHRGWPDFMRINPILGWHTSDVWYFIKWLQHASINTDFAIDYCSLYDDGYTSLGGCDNTVRNPQLKRKDSGKKDMLFWPAWWILDDDIERLSRVQKGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP85</accession>
    <name>A0A1E3NP85_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_71921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019024.1">
        <property type="nucleotide sequence ID" value="XM_019164048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP85"/>
    <dbReference type="GeneID" id="30180735"/>
    <dbReference type="OrthoDB" id="3994818at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="453" mass="52486" checksum="92A22A38528F9299" modified="2017-01-18" version="1">MWENKLVKASQSIVRCFSLSSRKCLFDKSPPKIPMISPLLNHRMLGKSHFMSTVDEETIIELLQNISEIPVSVSEVKYVNFADLPHSKRLPTLPKDLSEKSIVDYVNKLVQYRYPRSFTNQIGEALLEIVLSSPELLTKKHYLSIILFYHFHTNHKVGLQVLNLMIANTQIRQDIDFDNVLMSPTVRPPVYRQIIERLDTLKGKDFAANTNTWYYLFNVFKNPEPKIKMLELMGEYKVSLKPVLGSLSPIIEYFTPEQLTDLYQSEGCFLENGEIDPVLVNQLVACYLKDSRVDEVWSMIQNNSDLEKFITPGLFVVFANHFLKNNQLAFAIAFADYLQKNYKVKTTPILTSMILNVYLKDCPFFDRWLSLVRIFMPGVKRYHVFLNTKTISNLNDYCSLYKISNDFDVMEGQDFTTMKAIYSDLVWNDKPTFELSENSPDFIKTAKFIGQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPH9</accession>
    <name>A0A1E3NPH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71935</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019039.1">
        <property type="nucleotide sequence ID" value="XM_019164052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPH9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPH9"/>
    <dbReference type="GeneID" id="30180739"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="577" mass="65237" checksum="3FA54551A123B296" modified="2017-01-18" version="1">MLGRAQHRASAKVKVRRSKSGCINCRRRKKKCDEIKPICTGCLNRNIVCEYRQLSSDILRFAGEIDTPSLPASAAAGTQISSGKISSISSLRSLVGDTVMRILENNTSTLNSLFGSSGIAAVGAGGEGDDDGTTGAIASLDELLPYDTDFLNLIFEDQPQYLHLFSNPTPSIPGSLEIKLPVALSSEELSYFEFYCKSILPDLSILPQEFNYYSKIYVPIAFKEKSVLYTLVGWGCRMRKNVNLNFQIENVEADSYINKVNDLMSHNELSLDREKFITNFVCYMLLVCMEISFGDTSKWSHYFTCCFNMINKMPGNFRYLSSTCSIEGNLLAENFAYFDVLASQSNENGTFYPMSDYHDLFSQNNTTKIVHDPLQGCIRPVILLIGEIVSMLVEYSSLQVSLDLPECDKYELINKMLDRSDKLDKMIRFAKPDLSCLQYLNSPDELESHLTLFEVYQLSSQIYLRQVIRKIPPVVPEVQVLAYNLREDLKILVKSAKLKNSLAFPMLLLGISSTSNEDRKEVSKMFKSLIQSCGYLSSYQKLLIVVQKIWTLNNNGSLYVDWFRVTKQLGWRLNLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPL9</accession>
    <name>A0A1E3NPL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15830</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCC4/mau-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019079.1">
        <property type="nucleotide sequence ID" value="XM_019160985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPL9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPL9"/>
    <dbReference type="GeneID" id="30177672"/>
    <dbReference type="OrthoDB" id="3997784at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019440">
        <property type="entry name" value="MAU2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10345">
        <property type="entry name" value="Cohesin_load"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="746" mass="85820" checksum="14A1715DB31EE42A" modified="2017-01-18" version="1">MAKDQDVLVWQLVQASDFFLKSAHSIGAKALRDTSGNTLNEYNTLIKASLNCLFKILRHYSAILDAKIQSILYHKTAHILFKETISSDLALDYCIKGIQICKRNEPGLTVIKLKLQYLNFQIQFQSSSSPDSKKQALSYLNSILERELPEDPSYLNIRVFFSLIKLDYFSSIYTPERKISDLKKLCSMVESNLSKENYLFLQIILANLIELQLLNSISLSEVKESLSKLKGYQQPDNVFSVKIPIQFRGISLLFEVLLSLYEFQFDRCTENIQKVDSFVKSVKQSTLKTWSSRLNFNFSLDKSNSSFPVEFKWHSFKDFSLISYLYCGILCSFKSWEKKSKSDKILRLVSSSLITDSNQFSLTFEDAQNKKAKQSYLQILTTAYQLLSDLIKDKFPALENTDDTNPNSLTSVLQNNYHGLFSFIDSYEKSRYSAYECAVYDNLLPLIQYIFAMIYQRNGMFHKALYHYFMAHISCSKSNTKVNFLSSNSTSHSDNEVFNDPIYADFVQSNMGIFGTYSQSLGLSNEIYMLSTINALPIIQHLIEQQKQRQRGLTEFDIGYDTYLTRLLKLLKIKDSLSIQINQLLRTLNSTPLNPAATLSIPQITISTLCYFYTTEDYTFLSKLNLKEIEKASPLLTSFLYLIMGYTYKNDLGLTELENLNCRVGFYTSACNYSAKACNSSCINAIAQLGYLEIWKIMNHNKNLYTPESIGRVYQRFQHFAKDLTEIPDGSSDDQNRKRTKRVKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPS9</accession>
    <name>A0A1E3NPS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_31062</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018831.1">
        <property type="nucleotide sequence ID" value="XM_019162674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPS9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPS9"/>
    <dbReference type="GeneID" id="30179361"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021967">
        <property type="entry name" value="Nup98_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12110">
        <property type="entry name" value="Nup96"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="11">
        <location>
            <begin position="105"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="978" mass="112081" checksum="D69266AE395C027A" modified="2017-01-18" version="1">MNYSTWLGLDIPKIPASKSEYRRLVIRNPRDAFLKYDEIDANSVLLAKGVDLQFNSAEIAKPKSNSRKVVDELKPPSKRNSDEAKGEAESSDQTHKIELKAETTESDYWCTPSISDLSKLSSLELTHVQNFSAGRKNHGNLMFKYPVDLTAFEGRWDSLLSRTIVFQNRILQVYPDEAEKPSQGNGLNVPAVITLENVFPKKYDPKHPNFELLERHIERLKSAHGMKFISFDSLTGNYVFEVEHFSIWGIIDEEEDDPEIVARWLKQQELEQNNEKRKHELQINALEKIAGYGQPGDNWKRKKPDFGLITPGAIDFNKQENEIDDDILIPNNEGDALELLKTSPHLDDIDDLVEIRAYEPEVKDVDMQFIRPKTELAISDNWDEQLSLSNGFFSVFNRNLDQRHNAKLNPKHVGELIFGDKDVSKLKKAAIEPPFNFENSLHYQKCLQAEMFESEFTLRSNGLPQVVENLEISLHIPLLSFENSSDYNFWELLSIIYDDSYLKSFLSSQLVEECENLKPKLEYIQGLKRKELLCRFLQKLILLDLESNFGSSVFSRDTLDKIYHFVCTDKLADAIQYAVNTKNNHIAVLLTMLDSDDSTVHKLAKSQLTVWSQELVGFIPSSILKIYKLLSGEILSKEHIDHLEGLTWPVVLFLMIKFGDSNKPLNETIKEFVHYAEKTGISRLPIFQTHFTILKMMNSSRDVLATFDVELQFLLMKHLKKFIQFTAEEFDIIIQKFAEKLEKKAMTKEASFVLEHLNNDEIAKDKITKLFYNNVSNLGFLENDKKLNELHEILHIPDTLLHEARSAEYSNLGKFYKSTLELILAGNLCKAHDLTLEKVAPEIIIRNVPNELDRLSRLVEKFSTLSVSKVGSAVYGDYIKMIALSGAVDDDSNDYNAKKEKLRFAFDSVLNGITSLADTNQTVKVAKTIMIKKLVSVAFKEEINIDAEQFLRLELPESEKNYLEAKFDNPDGKKLLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPX4</accession>
    <name>A0A1E3NPX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_63005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019277.1">
        <property type="nucleotide sequence ID" value="XM_019163470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPX4"/>
    <dbReference type="GeneID" id="30180157"/>
    <dbReference type="OrthoDB" id="3996466at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="424" mass="46070" checksum="34A98169AD4440E3" modified="2017-01-18" version="1">MTQISDMSASHVRKELQQQSLTAQGSGAELHKSSHGASFNSMDTLSTASVSFPVLPLEGEGAGRAKTLEPLVAGKHIQTLEATGNNQVSGKSTETDCYTEANAPPSLKLPKRFSISKTDTQPGLPQDNTPGNMPSANGTPFPERQLYYLNALGQGSPEYHQQARALQPAYQQTFYNPQNGTLVTLPMSAPPLSYPNAVSATTAAPQPQQQPVVLMPVRPQTYYTRAANGQLVPVAVPTLSTIPAVCNMAANSQQTLQRNTTSDSTPFEETSPRKKQKTSAHPSPEIKKAEELQRQHIQRQTLLYPKLHQEVQSPNVSDIYQTNNESALAGSEDEHVEEDGVENSIKLEATNHAETCSTMGDTPIMVTGQDAEDKQPKLQKITGTVSLGAFTYKYSQTLSGDPIKDRELFDCLTDNAWKSCMAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ35</accession>
    <name>A0A1E3NQ35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="12">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ALA1</name>
        <name evidence="14" type="ORF">PICMEDRAFT_16022</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="12"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019323.1">
        <property type="nucleotide sequence ID" value="XM_019161060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ35"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ35"/>
    <dbReference type="GeneID" id="30177747"/>
    <dbReference type="OrthoDB" id="2423964at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070143">
        <property type="term" value="P:mitochondrial alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059090">
        <property type="entry name" value="ALA1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26023">
        <property type="entry name" value="ALA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 12" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="13">
        <location>
            <begin position="16"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="610"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="614"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="729"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="733"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="963" mass="106793" checksum="6FD6A4746E2D76A1" modified="2017-01-18" version="1">MSIYTNPATNPKVQEWPASKVRDTFLDFFKQKQHTFVPSSSVIPHDDPTLLFANAGMNQYKPIFLGTVDPSSSFSTLKRAVNSQKCIRAGGKHNDLEDVGRDSYHHTFFEMLGNWSFGDYFKKEAIQYSWELLTKVYGIPADRLYVTYFEGDEKAGLEPDIEAKELWSAIGVPDDHILPGNAKDNFWEMGDQGPCGPCSEIHYDRIGGRNAASLVNEDDPDVLEIWNNVFIQFNREPDGSLKPLPAKHIDTGLGFERLVSLLQDVRSNYDTDVFEPLFLKIQEITGVRPYSGKFGKDDVDGIDTAYRVLADHVRTLTFALADGGVPNNEGRGYVLRRILRRGARYTRKYMNYPIGTFFSQLAPTLIEQDKHIFPELAKDPSSIFEILDEEEASFARTLDRGEKLFEQYALIASKTPEQTLSGKDVWRLYDTYGFPVDLTRLMAEEAGLKIDEEGFEKARLASKEASKGGASKSAKALLKLDVHALALLGEQKVPTTDDSAKYDTKDINSQVLAIYTDGGFVDSVTADSFKDKQIGILLDKTPFYAEQGGQEYDTGRIVIDGKSEFHVENVQVYAGYVLHTGFIDEGELKVNDIVISGYDELRRWPLRNNHTGTHILNFALRDVIGDGVEQKGSLVASEKLRFDFSHKKSVELKELDQVEQIVNQIIKENLKVYAKPVALETAKSINGLRAVFGESYPDPVRVVSVGIPVEDLLADPSNEKWKKYSIEFCGGTHVAKTDDIKEFVIIEESGIAKGIRRIVAVSGSEAREVQRKAAEFSKILDDVSKLPYGETKERRVKDLGTELGKLSISVVEKAALKAKFTAIEKSVKDETKLRVKQDTTKVMDAVKSFFAAPDHKSVCAVHVSAGPNAKIISEAINYMKKEQKDSCLYIVLAEVEGKAAQGVYLSPAVQEKLPAVEVATKGAAFIGGRAGGKGNTCQGMGDKAEGADAAVEEVKTLLEKLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQM8</accession>
    <name>A0A1E3NQM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine protease</fullName>
            <ecNumber evidence="11">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_27168</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019504.1">
        <property type="nucleotide sequence ID" value="XM_019162484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQM8"/>
    <dbReference type="GeneID" id="30179171"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="12">
        <location>
            <begin position="1"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="291" mass="32954" checksum="3F7BE31407F61C16" modified="2017-01-18" version="1" fragment="single">QEFLDDLYTRLWFTYRSGFPIIERDTNGPSPLSIGSILRGTLDINNLGKGFTTDSGWGCMIRTSQSLLANALIALKLGRDWQFSSSSPENLEQHWEIVEQFADVPEAAYSIQNYVLYAAKYCGKRPGEWFGPSNAAKSIQKLCEEKSSVSNMKVYISTDSGDIFEDEVLKLSAENDSESFTPILILCGVRLGVQNINSLYWEFLKFLLQLSYSVGISGGRPSSSHYFFGYQSDYLFYLDPHVPQSAILKKILETIHSKKLRKLHISKIDPSMLIGFLIKSKSDYDDFKEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQT8</accession>
    <name>A0A1E3NQT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mitochondrial inner membrane protease ATP23</fullName>
            <ecNumber evidence="8">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14004</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Has a dual role in the assembly of mitochondrial ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Peripheral membrane protein</topology>
            <orientation evidence="1 8">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M76 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="8"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019538.1">
        <property type="nucleotide sequence ID" value="XM_019160179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQT8"/>
    <dbReference type="STRING" id="763406.A0A1E3NQT8"/>
    <dbReference type="GeneID" id="30176866"/>
    <dbReference type="OrthoDB" id="285308at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019165">
        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF0">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09768">
        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="210" mass="24191" checksum="F41BC55EF852C4BC" modified="2017-01-18" version="1">MSAEKQQEQQQVTTTPLLAQPEKPVLSEEKKASLCSQCDEYKQWMTQYSPTVRFMFDQIKQAGGYVSPNKINCQICEDPKLGGFHPDLGIQLCANYIPDKWVLNDTLSHELIHWYDNTRFKVDWMNLKHHACSEIRAASLSGECAIMTEFWKNSGVMRIAKGHQACVKRRAALSVVGHPKCKDMEHAKEVVDEVFRSCFNDTRPFEMIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQW0</accession>
    <name>A0A1E3NQW0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_121754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019076.1">
        <property type="nucleotide sequence ID" value="XM_019159894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQW0"/>
    <dbReference type="GeneID" id="30176581"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="122" mass="13415" checksum="ED53B18B8546B08B" modified="2017-01-18" version="1">MVLTLSARLFCSARKNNGGVLSEESKGFFSPAPRSVSGRRHAAPRVCHFCFTTGTSEHNVVGARRRPDWPGAHEAGWRGESRRKRKVLQKKARITRTGRIGRVGRIRAGTAGRRHKGLGVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQW9</accession>
    <name>A0A1E3NQW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_82975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019604.1">
        <property type="nucleotide sequence ID" value="XM_019164996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQW9"/>
    <dbReference type="GeneID" id="30181683"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="170" mass="19061" checksum="F13BCD2886892426" modified="2017-01-18" version="1">MSAAAAAGLNRKSAMAPGGMTKKRQKRKRLLESRAGHVMWHVSCGWERDWRTVAARHLGVWCPKTGKREKGEQGKKAKKRKREKGEKVRVSRLSAELCGAVALRQRGGIWLRWLTGRLRFSLVAKSCLPVPFHGATTNTRTNQYSHLSPLQPLATPPTLRYTTPRPAETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR37</accession>
    <name>A0A1E3NR37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_28861</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019668.1">
        <property type="nucleotide sequence ID" value="XM_019162561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR37"/>
    <dbReference type="STRING" id="763406.A0A1E3NR37"/>
    <dbReference type="GeneID" id="30179248"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106142">
        <property type="term" value="F:rRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="385" mass="43593" checksum="93F2AB00D51C404D" modified="2017-01-18" version="1">MSLFEVDGWGLEKRKLGLDKSSVDIEPKKPSNNSSGEDRSQDKQDRREKSGKRDKNDKKGKKRSKDDTDYEASEISEQNETKRQKSEIPPGVPDGIDPSNLTPLQRKMLSKLSGSRFRWINEQLYTTDSATALSMISKQPELFEEYHKGFASQVESWPENPVDTFTRELVYRGVNKMVSSPGGLPGVVVNGHRKVVVSDMGCGEANLAVQIDNFMEEYKKDGKQALKKFKKKYGGGKDAALKNKKLDFEVHSFDLKKINDKITVADIKNVPMADESCSVVIFCLSLMGTNFLDFIKEASRILMKGGELWITEIKSRLSDPKCVDFIKAIEGLGFKLRNFDDANKMFTKFEFFKPIKSAKVSNVGEIRNGQTEGKWLLKPCIYKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR58</accession>
    <name>A0A1E3NR58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_31570</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019281.1">
        <property type="nucleotide sequence ID" value="XM_019162710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR58"/>
    <dbReference type="STRING" id="763406.A0A1E3NR58"/>
    <dbReference type="GeneID" id="30179397"/>
    <dbReference type="OrthoDB" id="5580488at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043224">
        <property type="term" value="C:nuclear SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140299">
        <property type="term" value="F:molecular sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22147">
        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000715">
        <property type="entry name" value="F-box protein MET30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000987">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.16, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF9">
        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="108"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="247"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="288"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="367"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="512"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="587" mass="66328" checksum="4DF75A8EB2AED841" modified="2017-01-18" version="1">MATPSSTSAGQEKDVQKEQAYVLDTKYCYRHNPRIRCNKMADKQQLTEIQKKLSTFSKQDQQTVTNVWSSFANSSSQQRALILQGLISQCCFPQLSVISHEISHLLKIDFLEMLPSELALKILCYLDCQSLCSAAQVSTKWKQLADDDRVWRYMCEQHIDRKCPQCGWGLPLMAVKKLQEKSSRKRRFSEAEEEMKAVKNLPQPLWKCGASAPKRLMRKKRPWKAVYSERYTVERHWRKGFYSVKKFEGHTDGVLCCQYDNNKLLMTGSYDTSIKIWNTDTGELIRTLTGHTMGVKSLIYDDQKLISGGLDCTIKVWNWRTGECISSYTGHREGVISVDFLDKVIVSGSADKTIKVWHVDIGTCYTLRGHSDWVTCVKIHSKSRTLFSASDDSTVRLWDLETNKYIGTYGGVENGGHTAQVQCVIPFTYTDDLVTDVANCQKTSNAVNDETGGSNSEVAVFDSDDEEDDGNIENSNNFVKTRRNLPTHLLTASLDNTIKLWDVKTGKCIRTQFGHIEGVWSIAADTFRIVSGAHDKSIKIWDLQNGQCMHTLANASSVSCVGLGDSRIVCGLENGEVKMYCFDCNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRB6</accession>
    <name>A0A1E3NRB6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vps53 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_38384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VPS53 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019724.1">
        <property type="nucleotide sequence ID" value="XM_019163106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRB6"/>
    <dbReference type="STRING" id="763406.A0A1E3NRB6"/>
    <dbReference type="GeneID" id="30179793"/>
    <dbReference type="OrthoDB" id="10261632at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.110">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 53, C-terminus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031745">
        <property type="entry name" value="Vps53_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038260">
        <property type="entry name" value="Vps53_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16854">
        <property type="entry name" value="VPS53_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 C-terminal" evidence="9">
        <location>
            <begin position="610"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="83292" checksum="FCC168E7FC4B73C2" modified="2017-01-18" version="1" fragment="single">MIDIMELFDSPTALVDVNQLLKYTSSYESQLQAEIQKKRTEYKQILIESGPDQGIDVLDGEVMKLLDSFQNMKSLAEVTGNTINSMTANIKTLDQSKKNLTFTMTTLKRLQMLITAYDRLEKQLEKQKQVKDYSEIKQLLGAVLELNEYFQDFKSIDEVNRLNKLISAMKNQIINDIFKDFESEFNEELNNDKLIDACHILEILGDAYKEQLQTWYINTALKDITQIFRSSEEAGSLDNLSRRFIYFQNTLTNFESKHLSMFPKSWHMSLKLTEKFCSYTKNDLKEVLSKESRVKNSVDVNILLNSLSQTLDFESFLNKKFKYYKDFDEQLKNAEPPSFTKSISDVFEPYLNIWIDHQSSTIEKKLIEFTNPSHMFQKTGEGIADDDAKRSDESLNVLESAAELFRIYRQILSQLSKLTTGKALIRLSKIFSKYLIQYQHKILDTILPDSKSLVSVDLKSQQEGIDIISLVLNTADYCSQTVLQLEEKIKTLIEPKELSAQVEFEGPNNGFLQLIIYCINLLFYKIENDIQISWRELSNFNWKVLNEVTGESRYLGSLKSIIREDCQLIFKKISKIIYIRNLIDKLLEMLLKNIILNIIKLEPISVIMAEQFKLDLQELKSFINILPTLTEGGDRILSSTSFKSNINTKFKNIDNLMKVLMVPSKPMDAFINSYFSIIGDSSFSNFMKVLQLKGVLKTEATEKDKFRYMDMFKLQLGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRG6</accession>
    <name>A0A1E3NRG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">E2 ubiquitin-conjugating enzyme 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ubiquitin-protein ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_9187</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019772.1">
        <property type="nucleotide sequence ID" value="XM_019165075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRG6"/>
    <dbReference type="STRING" id="763406.A0A1E3NRG6"/>
    <dbReference type="GeneID" id="30181762"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990389">
        <property type="term" value="C:CUE1-UBC7 ubiquitin-conjugating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000837">
        <property type="term" value="C:Doa10p ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000839">
        <property type="term" value="C:Hrd1p ubiquitin ligase ERAD-L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180027">
        <property type="term" value="P:inner nuclear membrane-associated protein degradation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000008">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="9"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="175" mass="19841" checksum="E0B903063048B17F" modified="2017-01-18" version="1">MPPKGNYTTAHKRLLRELRELVENPVEGIIACPISDDDLFHWRCVITGPEDTPYEFGVFEARLDFPRDYPLSPPTMKFVTPLLHPNVYKDGKVCISILHEPGNDPLNYESADERWGPLQSIEKILLSVSSMIADPNPNSPANVDAAKLWRYNRDSYNKTVEQHVFESLGLKPPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRJ1</accession>
    <name>A0A1E3NRJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein SVP26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70308</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVP26 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019804.1">
        <property type="nucleotide sequence ID" value="XM_019163608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRJ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRJ1"/>
    <dbReference type="GeneID" id="30180295"/>
    <dbReference type="OrthoDB" id="28257at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097020">
        <property type="term" value="F:COPII receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007277">
        <property type="entry name" value="Svp26/Tex261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144:SF0">
        <property type="entry name" value="PROTEIN TEX261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144">
        <property type="entry name" value="TEX261 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04148">
        <property type="entry name" value="Erv26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26139" checksum="4137DF70B19E4068" modified="2017-01-18" version="1">MFIQVLAVLGSLTGFITITLSVAAGLYYFSEIIEENLQLTKRFLSRSILVVSAILILLYLFDGFPFKLTLFTLFSNYVYNLNLRKFPNIQLTNPVFVVSCLLAFVNHYLWFNYFNNPYIPSIDERLSAGFVPPYYPSFTEIASFFGICIWMFPFALFISISSNENGLPLSNSDLATDSSSLENNKNVQKSVNLVRSLIKKSLAFLSNLFNALGINLKFAKSDSSNPNELYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRN8</accession>
    <name>A0A1E3NRN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_9275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019863.1">
        <property type="nucleotide sequence ID" value="XM_019165083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRN8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRN8"/>
    <dbReference type="GeneID" id="30181770"/>
    <dbReference type="OrthoDB" id="5348546at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22699">
        <property type="entry name" value="FHA_PLM2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="136"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="616" mass="68131" checksum="27C196CF7AF18811" modified="2017-01-18" version="1">MNINSQFPPSSPLLHSENELDNIPFKDGFESLSKAKPYKNEQREKDRYDAYLTPDPSSSLGDVTITSDHSEADESMVKITADSSPSKISRVAPEAGFLQESKRAKPDGMSGIDVLQPLSTKNFQNVMYIPLNGDVFKIGRSGLSCSFKLNSSNKLISRVHAEVQFDTETQMIVLKCLGFNGLNITIPKLIGVEHIKDKEFLITVNKDDSEEENDNVNEATSSESRILTRSSAFTNFYMLKDETIRMPLIEGTVLDFRGDLALLVYKKDPLNLVSKTSTGPSSAPVDEPNKRKLSTGDLLKIVEEKKLQFSTHPTLAELKEKKDLSSSSSGARPIMRKNLANTIIYKRASPAPCEKRPADEAAAKKASESPKADAVSPVMSEPAKTVKKPLADITNKNKSTAAAASSTEKKKPVSVVSSSPVHRAAVSQESVPPLLPASDTPAVEKPIFREERSKTPEPKGAELPAEEQKKRGRAKKTKQSEEDILRSMPQAEVDAVLSTVPELANISNLVTNHIAYSRILQTPFSSICELNSIKKHNLTKLQLRCILIHSVACIGVIFRQGKDAAGKPLDEEYYYMPEKDSDIHRVQLVEELKGSSSHLRSCRKTHKQYFWKKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS20</accession>
    <name>A0A1E3NS20_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA wybutosine-synthesizing protein 4</fullName>
            <ecNumber evidence="5">2.1.1.290</ecNumber>
            <ecNumber evidence="4">2.3.1.231</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_70474</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine + CO2 = wybutosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37119"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="Rhea" id="RHEA-COMP:11847"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73544"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.3.1.231"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36903"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.1.1.290"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.290" evidence="5"/>
    <dbReference type="EC" id="2.3.1.231" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019992.1">
        <property type="nucleotide sequence ID" value="XM_019163650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS20"/>
    <dbReference type="STRING" id="763406.A0A1E3NS20"/>
    <dbReference type="GeneID" id="30180337"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13418">
        <property type="entry name" value="Beta-prop_TYW4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="700" mass="79197" checksum="038582B411D11816" modified="2017-01-18" version="1">MDIYSSVVQKQLGKNSKPDKEKKNEKQGKSRNKKVVHDLFVQGTNDSSIVSKRSVEILYNDVEASNSKPFFHHFVKKSPRRTPVINRGYWIRMKSIRMAIEKIIEQQPPEQRINIINLGCGYDPLPFQLLDEEKFQHRNLYCIDVDFPELIDYKSQMIRMAPELTTLIGPQLCDSESKIAPGVVIRTTNYATMGCDLTNRKLYCEQLDSFKANSPSTTNIFIAEVSLAYMTPETANPIIETSSTFSNSHFLILEQLLPAGENHPFGKRMIHHFNKMEAPLQCVHTYPTIADQINRFRKLGYNHVNAKDLLACWDLVPNDIKLKVKNVEAFDEWEEFIFFGHHYINLHATNQPNVTVYAEKNCELYPQMKSAATEYTVQYQNELPVNAQRKFHSCLGFPNASFLTCGIQQSRLSDTIQLKLSTDNDAVIQVPTDFKARVGATSIDTRSGSYLVGGRRIPGAGMDEVWKLHRVKANHYTWELKSPLLSGRVKHTAACINEEDILIFGGSDGETFEYYSNESNIWFKLDYSSDSIFLKGLESSKLVVLDTTNEIYLLGGMIYDPAGGFDFSSTVYQVELDLESKKVFLTEIVKHKSLARYGFNVVTTGSKILVIGGVGKTLYCQNDTIVEVDIDTKKVASVRINESLWRKSPVFVGSDVISRKSLNQSWIIGGGVVCYGFGSVWGGIVSINFGEQPSERIEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS55</accession>
    <name>A0A1E3NS55_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_57106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020080.1">
        <property type="nucleotide sequence ID" value="XM_019163215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS55"/>
    <dbReference type="GeneID" id="30179902"/>
    <dbReference type="OrthoDB" id="3986620at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="294" mass="32494" checksum="F114826E1F35B809" modified="2017-01-18" version="1">MLQASYTRLLNSNNFAAIGDSDRVYNTTPEGLAELVAGVAGDDAGDGAAKPSLPDYIGVANYCKSLVLNPAINSFNLQVDDIMRIWETRLLCMFIVNLAAKKHFGSTSSTGLVSDKMLQVEAGNVLDQVAYLNSATAGASAAGDVLQKQSTHPRSRKDVKLDRQFANLLLWIKHAGRDLVLLDHYYKEAFRARTSGQHDQDYLGRLQFAILGVLLRRKELFTAYTIANQENQLTSEQMLRVRDRIDNNTPDTDLFCLRLSLEPVTPGTEPPSADRNRSPSPLYRQLAQTLDACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS96</accession>
    <name>A0A1E3NS96_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_89605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020082.1">
        <property type="nucleotide sequence ID" value="XM_019165049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS96"/>
    <dbReference type="GeneID" id="30181736"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="134" mass="14943" checksum="8BD53ED80F74D73C" modified="2017-01-18" version="1">MYPSQQLVRVMPPSKRSRQNKLETQGATPMPCSNVGTIKKNFIIFSMCIVRRKKGKESKELNSGACKAHEKRRHILMPSSFIRCSSSGQCFDDPAGHNVKPSSIQYHHWHLSSSISAWLTFFSPSAGLTPSHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSE1</accession>
    <name>A0A1E3NSE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor eIF2 assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CDC123 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020132.1">
        <property type="nucleotide sequence ID" value="XM_019163685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSE1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSE1"/>
    <dbReference type="GeneID" id="30180372"/>
    <dbReference type="OrthoDB" id="360540at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905143">
        <property type="term" value="P:eukaryotic translation initiation factor 2 complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007807">
        <property type="entry name" value="Cdc123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="361" mass="41962" checksum="8843200C49C46AB5" modified="2017-01-18" version="1">MTDGHFVVIPVTVEDVLYCSFSSWYAKFGKYTISDAAIFKPLPKEFVAYLESDGIELPKENKKDSVLFQDAKPNSDNEYSDWEESDAGILKNSEKMNSDPTEHFKSFHNELKETIKSFGSVAPKLNWSAPQDSTWIMTGRNMKCFSTSDLFLLLKSSDYVNHDMYHAFDETTDYDKANPPKFELELILRKWVDINPSTEFRCFVRDRQLIAISQRDLNYYSFLDEIKEEIMEKIFMFFDDVLMGKFDCNSFVFDVYIPKPLKKMYLIDINPFQRSTESLLFSWNELATMKPADDDVDIRFVTEHNSGRFASKAHSQNHVPVEFVNASVDTQSMIDFLKKCKNMELENSDSDDSSNEETERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSK9</accession>
    <name>A0A1E3NSK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70189</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019676.1">
        <property type="nucleotide sequence ID" value="XM_019163583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSK9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSK9"/>
    <dbReference type="GeneID" id="30180270"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF12">
        <property type="entry name" value="ASPARTIC PROTEINASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133496" description="Peptidase A1 domain-containing protein" evidence="10">
        <location>
            <begin position="20"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="78"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="564" mass="61457" checksum="641257519A30DE80" modified="2017-01-18" version="1">MVMLKQLLVASVLAVFAESAFVHVPVVKKRHGGSDTLGDGVPGAGQPHFLLKNKNRVVKRDHPDASFEMELRFDQLMYEAQLEIGSNKDKVILLVDTGSSDMIVNSRSNNECLADGEDDDSDDGNGINGGNENLSSSDENFDNNIGNFCKREPPSVEVSRTHPVYPSITRSISSGPTDEAYLNRIGSKLAQVFNCMVYGVYNQDNSTTMSNLTTPLSITYADGTGANGFFVSDDVYFGDTKIETVTFGLNINTYKQNGVLGLGFKANQSPFQHGYAQYDSFPYRLKTLGIIDKVVYSFCAPYGGSSTDSIIFGAYDSNGYDHSTGLTLVPIVDYTVSPKVGDGPYYISITLNSISFTSPGVNNKLVASGNAPAILDLGTTSSVFPYYIFNEIITRFNFQWSSQLNSYVVSESEIEKESSYVTFNFQNAFIKVPVIDFTYPVIDGDTLSATGLRSISISYTNDDYFLFGDDLLSSVFFVVDLEEKNIALGQVNTSGGESSDSIVVVTDGIVDAVKSSNWDYVYGYEGSTNLKLKEAENPDNIKTAQQSGSNLQLYIAGLGTQLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSP6</accession>
    <name>A0A1E3NSP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020193.1">
        <property type="nucleotide sequence ID" value="XM_019160435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSP6"/>
    <dbReference type="STRING" id="763406.A0A1E3NSP6"/>
    <dbReference type="GeneID" id="30177122"/>
    <dbReference type="OrthoDB" id="275748at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="16"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="110" mass="12422" checksum="4EDB4A4BCDAF0A0B" modified="2017-01-18" version="1">MSNSQKHYQMNQQQFPIVQLKNLSFNHASSDLFALMGQFGNIQEIRVGSASNVETRGQALVVYTTLKAASLAVEKLSGFNYHGRYLIVRMYTPEPHLWKELSENGTVSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSS8</accession>
    <name>A0A1E3NSS8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70754</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020308.1">
        <property type="nucleotide sequence ID" value="XM_019163729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSS8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSS8"/>
    <dbReference type="GeneID" id="30180416"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015188">
        <property type="term" value="F:L-isoleucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090513">
        <property type="term" value="P:L-histidine transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF349">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="195"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="587" mass="64223" checksum="CC59B5E7DC7BDF38" modified="2017-01-18" version="1">MSDGAGTPTPYSPLHHEHDDNRHRVGSSYQAIPIPDRSARKSRSHFFSSVASPGNINSLAAFANSYQRAQSFRSISIEPRITASRSYFKDDEELIDPSTFAPSPMGRKISTVFAVRPYTHSPNTTFDEAAIDDQHSFLDHSFTDYVASRQPSFASSIFPPPGSGLSRALSDEVGSVVLRQVETHDGTKLTMLAPQSTIPQTIFNCINALIGIGLLALSRAMVHAGLYCGTVLIIYSVAITYWTATLLSDCMDTDPSICTYADIGYKAYGPKARLFVSLLFTIELLGVGVSLMVLFADSLHALFPEISLIQFKWIAFVILTPFSFFPLRVLSHISLIGIISTISLVFLIFFCGIIKKTAPGSLIDPAYTSYYPASALSLFVSYGIILGPFGSHSLFPALKADLVSPHKFKSCLKTTYSVGFFADASMAYLGFFMFGAGLLNEITQSVLLTTGYPKFVYFLASVFVSLIPVAKTPINALPIINITEFLFNITPQQLEYAGKPETLRTKFASALIKIGVNLTFVVLGILYPEFDKIIGLSGSSLCTLICIILPCAFYLKLCKPRNKWFYYLVMVVALILGVVGTVSAFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT63</accession>
    <name>A0A1E3NT63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_28948</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020412.1">
        <property type="nucleotide sequence ID" value="XM_019162565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT63"/>
    <dbReference type="STRING" id="763406.A0A1E3NT63"/>
    <dbReference type="GeneID" id="30179252"/>
    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010828">
        <property type="term" value="P:positive regulation of D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000273">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="57"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="127" mass="14657" checksum="61F31791D759EA78" modified="2017-01-18" version="1">MDIENENENDSQSEVIGELPEGKKIDENKSNSKKKRFEIKRWTAVAFWSWDQTNETCAICRNHLMEPCIECQGKNEDHGVGTHIECPRAIGRCNHSFHLHCISTWIQTRNSCPLDSAEWSLKEVVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTD4</accession>
    <name>A0A1E3NTD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Elongin-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_29142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020474.1">
        <property type="nucleotide sequence ID" value="XM_019162574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTD4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTD4"/>
    <dbReference type="GeneID" id="30179261"/>
    <dbReference type="OrthoDB" id="21513at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051870">
        <property type="entry name" value="Elongin-A_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010684">
        <property type="entry name" value="RNA_pol_II_trans_fac_SIII_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141:SF76">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06881">
        <property type="entry name" value="Elongin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30053" checksum="EA8525D4EAB2E0AB" modified="2017-01-18" version="1">MKNGVLSLTHIARLKCINNAHIINDVGTTPYHLIEPVLLKKTAKSLRQIESVSPQIIADSEPLWKSLVKRDFPDRPVNQTVLRSGKKVNVSSRSLYDKYFSEREAQRQNATNNIKQIVRNLNEVKNRNKVKAVNKVFASNKPRLAHNRPASNQQHPFKSSLLQKARVANKQRVRNFSQAKNSNLRIPNSKIDLVKIGTAHRNIVRNDLRNSPGAGRPSDVTTGGSKRRTDLSAAAAPTGPDVKRQKISDSSREKSKKSNTYIYARGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTP6</accession>
    <name>A0A1E3NTP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ceramide very long chain fatty acid hydroxylase</fullName>
            <ecNumber evidence="18">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PICMEDRAFT_57539</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Ceramide hydroxylase involved in the hydroxylation of sphingolipid-associated very long chain fatty acids. Postulated to hydroxylate the very long chain fatty acid of dihydroceramides and phytoceramides at C-2.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 2 Zn(2+) ions per subunit that likely form a catalytic dimetal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the sterol desaturase family. SCS7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="18"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020634.1">
        <property type="nucleotide sequence ID" value="XM_019163244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTP6"/>
    <dbReference type="STRING" id="763406.A0A1E3NTP6"/>
    <dbReference type="GeneID" id="30179931"/>
    <dbReference type="OrthoDB" id="2204368at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102772">
        <property type="term" value="F:sphingolipid C4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000038">
        <property type="term" value="P:very long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005149">
        <property type="entry name" value="IPC-B_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17 18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10 18" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 20" id="KW-0349">Heme</keyword>
    <keyword evidence="14 18 20" id="KW-0408">Iron</keyword>
    <keyword evidence="5 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 19" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="22">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ49521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="44177" checksum="71580EA14DC6515A" modified="2017-01-18" version="1">MALPLLSRKAIEAHNTETSCWVTLYHRKVYDISGFLEEHPAGPEVIMEYAGKDVTEILADPESHVHSESAYEMLDDGMLVGYLATEEEERELLAKHADMEVVPDVGVGEDGMRIADLTEFGDIPDDLLHVKTDHSEDYKKHKFLDLDKPLIMQVLRSNWTREFYIDQIHRPRHYGKGSAQLFGNFLEPLSLTPWWVVPSVWLPVDFYILYVAATHLSPIVTLTFWAIGLFVWTLIEYCMHRFLFHLDDNLPDNSVAFTFHFLMHGVHHYLPMDKMRLVMPPTLFCVLAFPFYKVIFGIFPFYIACAGWAGGFLGYIMYDVTHYFLHHVKLPAYIQKLKKYHLEHHYKNYRLGFGVTSMFWDRVFHTYLNPDDVYEKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTR8</accession>
    <name>A0A1E3NTR8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14557</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020181.1">
        <property type="nucleotide sequence ID" value="XM_019160431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTR8"/>
    <dbReference type="GeneID" id="30177118"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170071">
        <property type="term" value="C:CROP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044090">
        <property type="term" value="P:positive regulation of vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="406"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="595"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="605"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="670" mass="73857" checksum="D6EFB09FDC014969" modified="2017-01-18" version="1">MQQNMEDSSVYSMQNDSTMMSVKLAPSEPSENITGLNFANFNQDYSCISVGYENGFRVYNCDPFTQCYEHLDGPIGIVEMLFCSSLLAIVGTGEQASLSPRRLKIINTKRHTTICELTFPTSILSIRMNRERLIVLLEEIIYIYDITNMRLLHTVEIPPNPMGLVALSINSDNNYLAYPSPQKVTKLNGSNSVPNGPGVDVTGINGQHPSASNASHLPNTGVNSHDDLAGLHDTSVTQSIRNGDVIIFDCKSLQPITVIDAHKTQLSTMAFSQDGTLLATASDKGTIVRVFSVERGVKLYQFRRGTYNTKIYSLAFSPRNMFLVASSATGTVHVFRLGEEEAKNTVVKSTRSSGWLNQRKNENDRNLEVTRQNELDRLKNHQAEMDTVDDFELTNDEDQTEVDVADFQRLGIHSDSEDDIDSSDDDDDDDEEDEDEDEEEGEGRHELVNNVDDNVVESAAVGKTMRGTEEDFENVDVLRPLPSELTNNTQSGAISIFSSSSGGSTSGKAQPIVDSNRRSVARMFRRTSQSLGRKAAEKMGTYLPPRFSSILEPNRHFASFKVPTSKENMSIVGVIELNVDSSIDANDELSIQSHTGHTLHDDSSSTHSLNSGVSGLSNGSRGNSRSNSRFSKKKAVHILVVSADGIFYTYGLNPERGGDCVLLMQRSLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTV9</accession>
    <name>A0A1E3NTV9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020700.1">
        <property type="nucleotide sequence ID" value="XM_019163836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTV9"/>
    <dbReference type="GeneID" id="30180523"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="130" mass="13617" checksum="0A577858F860D8D4" modified="2017-01-18" version="1" fragment="single">MNLAANEAQKLPDISTNTFDYQESDRAIPLVDPPSENPASVPSDEDHIDASSEPYFPSTSLPDCVPDAVPSVVPVLNPLPRVSSQLSLSSVASDTELLGLLQDDSPPDASANQAITDFSLALDLTSGPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUS3</accession>
    <name>A0A1E3NUS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_14862</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020511.1">
        <property type="nucleotide sequence ID" value="XM_019160571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NUS3"/>
    <dbReference type="GeneID" id="30177258"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140836">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat S5 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000554">
        <property type="entry name" value="CMGC/CDK/CDK7 protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="7"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="13"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ49398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="297" mass="33529" checksum="4BED690C6B00E9D7" modified="2017-01-18" version="1">MSGIQNYSKTKKLGEGTYAVVYLGTQLSTSREVAIKEIKTEGFKDGLDMSAIREVKYLQELRHANIIELVDVFSDSRQNLNLVLEVLPSDLEKVLRDKNTFIGGVDIKRWLLMILRGVYHCHRNGILHRDLKPNNLLMDPSGNLKIADFGLARSLDQPTEKLTSNVVTRWYRAPELLFGARLYTAAVDVWAIGTIFAEMMLRTPYLPGSDDANQLAVTIQALGTPTEENWPGVSHLPLYNNLTVYPAPTRAELRRRFVAASEMALEAMEGMLIMNPSERWTCERALQSGYFLEEPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV47</accession>
    <name>A0A1E3NV47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">V-type proton ATPase subunit d</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 11">Vacuolar proton pump subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_14973</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020651.1">
        <property type="nucleotide sequence ID" value="XM_019160615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV47"/>
    <dbReference type="STRING" id="763406.A0A1E3NV47"/>
    <dbReference type="GeneID" id="30177302"/>
    <dbReference type="OrthoDB" id="7676488at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3240:FF:000002">
        <property type="entry name" value="Vacuolar atp synthase subunit d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="285" mass="32060" checksum="A1AC12953F5A1603" modified="2017-01-18" version="1">MSGAANREQVFPTRMTLGVMKGKLKGAQTGHSLLKRKSEALTKRFRDITKRIDDAKRKMGRVMQTAAFSLAEVSYATGGNIGFQVQESISNNARFKVKARQENVSGVYLPQFEADINESINDFRMTGLGRGGQQVQKAREVYSKAVETLVDLASLQTAFVILDDVIKVTNRRVNAIEHVIIPRTENTIAYINSELDELDREEFYRLKKVQEKKQIATAAADAEMAAKKLREEAERLNELEKHEAEDVEKLVVKSEEDNEDEKEAGEEKTVDTTELVTETEGDVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDD2</accession>
    <name>A0A1E3NDD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_74966</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015243.1">
        <property type="nucleotide sequence ID" value="XM_019164928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDD2"/>
    <dbReference type="STRING" id="763406.A0A1E3NDD2"/>
    <dbReference type="GeneID" id="30181615"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF34">
        <property type="entry name" value="PROTEIN HOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60148" checksum="51E71D13CC1B3064" modified="2017-01-18" version="1">MLFGRDKKIKTDISVPGTTVLVGKAAEIKLLGLHEEGDSEIILFPQPSADPNDPLNWPSWRKHLHFGILFFFGLILAAASNFVGPIYTLLAEIYNVSLSQLNTGGALEFLFLAFSCLFCQPVAGKLGRRAVYLFSTLLTIISAVVFVGDKTYGGYLGYSILIGCAVGPIDSLIEVSITDIFFLHQHGKYIGIYSLTMGLGSAFGPFIAGYVTQNLGYLWCGYLLIIIAGGLLLVEAFLLEESVFKRTYESPELEDKLLEIVISNTSHVKSHTADIDPATKNNAVVELDSVSEPATIPRPKTYIELLRPFTITDTRFTPMTVVNTLKVLRYPAVLWCSIVYGIQICWLSLITVSESEFFMAPPYLFSANSVGLLSMAMVIGTVIGGAYTSCSDMVQLYFTRKNDGIFEPEFRLLMLPIPIFLNVVGLFMYGLGPYYGVHWIVGAIGIVFIAIALCCISGLSLNYVLECYPKQASATMTSVLFVRNLMGMVFTWVFQYWLDNVGVIGTTSMLAAFCLVINGSFIIIYLWGKNFRKYTQKWYENAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDG3</accession>
    <name>A0A1E3NDG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015278.1">
        <property type="nucleotide sequence ID" value="XM_019164864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDG3"/>
    <dbReference type="GeneID" id="30181551"/>
    <dbReference type="OrthoDB" id="3996886at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="131" mass="14787" checksum="721A33624C3DCE9A" modified="2017-01-18" version="1">MGLFNIIEEKFHNDKNNKNRMMSTINNQQKFGLHTGDSPRNRMMDSISNEQGAAIVGNRKVPDLEYSSTVINKNLMTHTISNEQADDLACGIDIHRDHSAPRNKLLDMKPNESFGRSEPKFASGLNSDRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDH6</accession>
    <name>A0A1E3NDH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_13738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015298.1">
        <property type="nucleotide sequence ID" value="XM_019160103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDH6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDH6"/>
    <dbReference type="GeneID" id="30176790"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF3">
        <property type="entry name" value="HALOTOLERANCE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="7">
        <location>
            <begin position="371"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="88369" checksum="F5D2D54A4C5A8AF3" modified="2017-01-18" version="1">MKKAADDALVESTDSETPAVKRRKAVFSCDRCKTRKRKCVRFINNDEGEKTSTFDIVYPCEECIQSKSICHTYLGQRKQTTLKKLSLTESALTKDCVGQDSEAKMKELEIKCQYLVSLLKQVSPDIKTDLFQDDDTSPLSVASTSSSDQSSLALELHSKPHKLPGAMQMEEIKEFSSPIDERKSLSHDDYIGPLGSSTLHETVIGLIGSPGSLRSSSTDLTHSVTGENLLLGNDMRLKFYDNNQLVYSIPRQEADLYVCEFFDRIHLICNCIDKEEFVRIYELFWLLLKHEDLRSINSTLFLSSTQICTIYLIWILGKRFKHRDDMSLSDTYMSIIQTTLSEIILRPSEAGIQMAILFSLYLESTHSRESAWIVIETAVTQAISIGMNKVKSSSVRDIDEKNKGYSKIWCTLFAQEVRLSNVMGRQSSIDFTEADVRLPKFQQLKYSNLPKYWVQQINSEEYQLYFNKYYELNKIFHEFLRYRSSVMEGTSIYTISSIKKAWELRSKFHAWINGLPPTLKNIFDKPPTSIFNYQVYLHLTYHYYFINLGLPFLMLILRIMQSERHIKLKKTDPIFVLAISAISCSKQLFTIINFEFEKDLFNGSVYNDIYILYHSTLIFTIAIVVVSNKNAESILDLKYLEAKKNVSKEKIWHIISKIGELNKKLQPLTSGSNREVSQNIEMLVENVSHFLAYDRADGKGDIQALDQSPVHTPNSKPFNNKLDSLFHTMNMNTDELWKMINWDWNSYEGGDVLFKEFNELDSYFKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE41</accession>
    <name>A0A1E3NE41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Protein PNS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_18312</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Probably involved in transport through the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cell membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015513.1">
        <property type="nucleotide sequence ID" value="XM_019161944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE41"/>
    <dbReference type="GeneID" id="30178631"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF4">
        <property type="entry name" value="PROTEIN PNS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="493" mass="53479" checksum="9515CDD8B7A33085" modified="2017-01-18" version="1">MQLEAGEEAAEEALEEETWPGEHARVSDAGFVVLFAGTCVAYCYCWLHVCDTASVGALLAPPETQHGHRHPPRTLPLLPLRPALPLLRHSPLLEPPRTVSAPVSSPVVAGLVIAWSTVALGLVVMAGAAKCCSSYAVFLRACHCTSSALLAAVAVYCVLHHHTYPGVVLLCAAAALLLCRRVLAAKFQRSAAVAALVASVARHSTSLALVASAGAVLALLVRLTWIVLLSQIYYKWEHLIRFHAGTFADPGMLLVTAFSLGSGAYLADVVCNITLLALSGMYASWYWRREISFRHTRTAVKNALLLKAGSACFASFFVSVSELLTLMHSILLAVFFVKRTTCLEKKLALTNSYVLTYVALSDLSIVGSVDRVSQSLGPEPWAALQKDGIVNHTLQICSFTISLLTAVVCHLYLAHRQPGFGQHHNFTILFPLFGFLLALEMCRMAFLIFTAGNNTLIVALKSHPEVANRGVSTNYRSDLLHIQEQWPTLNPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE92</accession>
    <name>A0A1E3NE92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_74657</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015529.1">
        <property type="nucleotide sequence ID" value="XM_019164835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE92"/>
    <dbReference type="STRING" id="763406.A0A1E3NE92"/>
    <dbReference type="GeneID" id="30181522"/>
    <dbReference type="OrthoDB" id="15808at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070183">
        <property type="term" value="P:mitochondrial tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000002">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050203">
        <property type="entry name" value="Trp-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="390" mass="43771" checksum="F9C51F1B121371BF" modified="2017-01-18" version="1">MKITQYTLQGLGSLVKARSYTRVSSASTVQTVSVTDPFFRLPNQSTILSGIQPTGTFHLGNYFGAIKSWHNLNTLVANSSNDASLFFFVADLHSLTVPQNYELLRQQRLEAFASIIACGVNPESSTLYYQSTIPEISSIQWVFSCITSMGYLNRMTQWKSKANLNESASVSDDSALGKVKLGLFTYPVLMSSDVLTFRATHVPVGQDQAQHLELTRETVHAFNKTVGQDYFKVPHTMLTPTKKVLSLKDPMKKMSKSDKEPLSKLFITDSPEEIRKKFNKATTDSVEGKLTFDERNRPGISNLISILAAATNSSIEETVKEVEHFTKKELKDRVAEEVIRELEEPARKFKELIANPELLERWSQRGTDKARSVANETLKDVMRLVGMGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NED6</accession>
    <name>A0A1E3NED6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_18381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015595.1">
        <property type="nucleotide sequence ID" value="XM_019161977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NED6"/>
    <dbReference type="GeneID" id="30178664"/>
    <dbReference type="OrthoDB" id="3997871at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="266" mass="29737" checksum="884C125844FBFC70" modified="2017-01-18" version="1">MSQRIPDLRSSVAASEDTVTLLQNRLSLVNANYTLSQSQSQSQSQASQSQLQNPDAERAQKLRVLALLRQLKTVPLKSVSSKTVPASLANCQLRTQTRQLSNTLSADVVASDLFICRSIAAMLESLLAALNASIADLQSQPSFRQQPDEIKASILLSKTDALKARARKLSSYIKVLVNDYLFGLEFSFLFKNLDEDSLKSRKQKFLKLLEVLLNNNILHPNSEHPTWVTLNSMDDPLVRFLLLNRMILVHPTIPNKIKLKDLSVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEF3</accession>
    <name>A0A1E3NEF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015617.1">
        <property type="nucleotide sequence ID" value="XM_019160098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEF3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEF3"/>
    <dbReference type="GeneID" id="30176785"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="383"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="582"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="621"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="776"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="790" mass="88908" checksum="A1891ABC0AAE715C" modified="2017-01-18" version="1">MMDIDSPSSRPDNISSLGESPSFTASLRSNSFGLNLTNNLKRPLLHKKAATQIHNGCFNNDFTFSTYNKENSFPSASSTSSVSASATTTSTNPLYKSFAFNGNPVISKKSSVKKQMKKKSSSNLFRSSQNALCHGHTLNHSHNHSHGHNRSNSVSHRHTKSNSSISSSSIFKNLSTKPSIDSIFNFSQETQPLNPSSKPIFSQSNNTFIVSPASNLSTPNGSENDSYYSSDDNVSDDEDHNIVLADESFDNDESDSSNLDFGSPLQKKQPNRTELLSNITHFKLQRHQSLMQFGVSGKNTNHPLNNQNYKFDTDQLASNINGLSVNSDDDDSIYGNSMLHDVPFEFSSNEYGKIPRITVDQFKKILHEYKGKSNAVNGKFCKHFDRLIIVDCRFKFEFKGGHIDGAINISSFTELENVFFTDEILQKTPLEFNKEERTLLIFHCEFSSHRGPMKADQLRYFDRSICGDFYPNLYYPDMVVLEGGYKDYYESEKSLHKSLSYIEMDNPLFKDERERNLTLLRRESSLSRKNSRNSSSVSLSRKTSHSSIKSINSSSILDFSSVNKVEFNQTLKPRPSKRFLPNANSSSTQSLLLNNSNGSTSTFSSDCHKLEIPKPQEQNDEPYTSSSSTPTEYGLFGGFGNHKIDLDHLVDEDDDDFETDSIQIFEDQGDSLKNTLRTSRLPYGGNEGFKVPLPRPKFNRRGSLHLRSNTVSSFSFSNHSGFKSVPLLSTPEKAEAKFANNVFFRHNNNSSVYSVQEGNENDSPNNTEYKSIYSNKHNEKHYDTPIKAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEX5</accession>
    <name>A0A1E3NEX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_36684</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015787.1">
        <property type="nucleotide sequence ID" value="XM_019163025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEX5"/>
    <dbReference type="GeneID" id="30179712"/>
    <dbReference type="OrthoDB" id="10254627at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_uS9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001099">
        <property type="entry name" value="PRK00132.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="248"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="263" mass="30068" checksum="4D2C9779EFC0B7D8" modified="2017-01-18" version="1">MFQPQNRLYNDPLVSRFIPELEARRLVPAIPTFYSRNPYHEMHMSKVQDLLNKNATLPFDRRTSTGSWLRFNQYKERAGGELLKETEYLQLVNVLKRLDAIDVELRSDELNSVLAEYMRKQNVNQAASDLKTLDDKGRAVAVGRRKASSAKIYVVEGDGQVMVNGKSLEIVFPKEQDRMKLLYPLQIVESESKFNIFGLVRGGGSTGQIEALQLALAKSLCIHNPLYKQRLSAAGVLHRDPRSVERKKPGKKKARKMPTWVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEY0</accession>
    <name>A0A1E3NEY0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015241.1">
        <property type="nucleotide sequence ID" value="XM_019160119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEY0"/>
    <dbReference type="STRING" id="763406.A0A1E3NEY0"/>
    <dbReference type="GeneID" id="30176806"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="530" mass="60644" checksum="6B7AEF40EECD8CE5" modified="2017-01-18" version="1">MPLRKAIKRTRTGCITCRLSKKKCDEIRPNCSTCARKGVSCVWPEEPSQTLNQYISKKGTDEFHDHISKRGRRTRRGCNQCKKIKKKCDETKPWCTRCVKKGVTCTWQEQKDGGEPSSDAAELLFSVSEDESDLNATETCSLIRTPDNNYMPFNDVKIRINDESSQIFLFKIFFNNIIDGLIPQKSLQSLSDLTFESLKNSPEFRNVVASLSAAFVYTVDDSKRNLVESMHDEALLTVMNLQTNDVTIEAKLHSLDFCILRALYTKTNNKELLRYLNMALDLTLQYTPRTPNLTFKVIVESLIYHFSVSRIVVPVEALDLRNPFIICGALRHYFPQYPDSQSNPLLGSALDIFISVAKVSYVFKKQDVSFEIQRLLLEEIECLMALQPMRAAPFAVADSFEENDSILVSIPSAKYITLLSCKLLLLHILPETVAGEFDAIVRDTISVLKRSRAYSCEVEICGLWGMFMLGINLKNTEDQEFMAEYFTQLWEKSHNLGYLKSISHMRYAWEKDVGFEILRDKNFSQDLCLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF04</accession>
    <name>A0A1E3NF04_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18129</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015848.1">
        <property type="nucleotide sequence ID" value="XM_019161888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF04"/>
    <dbReference type="STRING" id="763406.A0A1E3NF04"/>
    <dbReference type="GeneID" id="30178575"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="59"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="5">
        <location>
            <begin position="60"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="116"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="193"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="246"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="299"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="355"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="420"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="496" mass="54407" checksum="18DAA4428D4EDC65" modified="2017-01-18" version="1">MAKLVRKRKSASLVSEGNVTPSPAKKARSDAQHHHSFESIRASRYKTINDKPTAKSTPLDLFVWGSGSMCELGLGPDAKTKEVKRPRLNPFLKKEEVGIVDFAVGGMHVLTLDKSNNLWSWGTNDSGVLGRDTSGQADKNLRDIDSVKDEDDEDGDLNEAESTPGKVEGLPKDDQIVRVAASDNLSVVLLESGQVWAWGTFRNNEGILGFSKDVKIQKTPVRIEELENIVQIVAGKDHILALDSKGLVYAWGNGQQFQLGRKIMERSMMSSLEPRSFGLKNIKFIGSGEFHSFAITRDGKVLAWGLNQYGQCGVDMNIEDGAVVGKPTEIKSLKGKKIVYITGGEHHSIAVSESGELYTFGRYDMKEIGIEKEKLPMDDCIVDAHGNVRCLPVPTKLTTLPPVNTVACGSHHSLAITKDGLVFAWGFADTYALGLGNLDEDIAKPTRIDNTATREHQIEIVGCGGQFSVSAGIKLDDEKVEERLDKIEQFEEELEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF16</accession>
    <name>A0A1E3NF16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_18126</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015844.1">
        <property type="nucleotide sequence ID" value="XM_019161886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF16"/>
    <dbReference type="STRING" id="763406.A0A1E3NF16"/>
    <dbReference type="GeneID" id="30178573"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="112"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="87882" checksum="7092C58D669E345E" modified="2017-01-18" version="1">MAVEKVGNMPKAQMTGPGASAVTGGAGKPGPGVGLRIKRRRRRLLLVFAFLLTATLAWYTLLKGRSSIDADQSLLLKAKEQGAGNSNINSNSVDGGSSADENGNIIGALFDIDAVNKYFNRLTEETKKAEEQTEKKESQLLAFVNDESEALLNDAEELRKLYLSKEHNGMINLGSTGNMADAQLNVLKLKNKMSELEALEVKENLDKAKDSLKLNSANKDRSIPLSDSLIPDEESFSKIKTDDFIKGDITFQNFFSHILELISRNHLSFPLKRRMTLENGKPVIDNVLFYEDTAETLSEQQLYTFFDFPQNFIDDLKMKHENVINGIPDIAPHFYNGNGYVAVGGGIYSWYALLGIETLRKVGSTLPVEIFLPNESDYDYQYCEKILPQLNAKCIEMHRVFGSEGLKNFQVEGYQYKVFALLASSFENAFLMDSDTYAVSNPDVLFDSELYENYKMITWPDFWRRTTSPVFYEIRGTEIGMVPVRHLNDFFVNPEFLSKPEDDVSTAVTFHDRAGTIPEWTTESGEMLINKKEHFKTLILALYYNYDGPYGYYPLLSQGGAGEGDKETFVAAANFYNKKYYQVYKKPERFFGWFNNVQNYEHSTIVQYDPLLDYEMLQKVHTQLRQDMESQKEDFVYDYNKYYSDVFTPDVCKPIFYHVHDPKLNPYKIMEEKWTENLEGKKIRNLAEDFPRINFDLERFLWNVMNHYLCELKIKIPAFEGKDADKMCNEFMVDELAYLDKSSEKIEAVYSPTNNVEQMHGGRDWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF40</accession>
    <name>A0A1E3NF40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_18133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015855.1">
        <property type="nucleotide sequence ID" value="XM_019161890.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NF40"/>
    <dbReference type="GeneID" id="30178577"/>
    <dbReference type="OrthoDB" id="332390at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075:SF6">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAC" evidence="6">
        <location>
            <begin position="25"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="DDT" evidence="5">
        <location>
            <begin position="650"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="248"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="524"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="737"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1086"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1246"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1477"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="577"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="737"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="755"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="790"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="811"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1091"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1246"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1261"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1289"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1305"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1384"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1491"/>
            <end position="1516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1516" mass="174170" checksum="24E807304363E1C9" modified="2017-01-18" version="1">MVLFKRKQITFVPPPLLPADATPSTPVWVIPETGEWFLKYEDYLNRMSFYNVKKFVCETSGNSNFTFFEALKVEHNELFSMQSNFPEPVKEPILRHVSFSTVPRIDLLVDQVYTKFKDDFFPGDKVIVKYTGGYRAHGIVKEKVVFNSRMDSNGVIQSPFISYRIFLPADENEVVIEDASLIYRERNRFTKSYVKTFLKMSLVRSNRLGAPWVIRDEIAKKYKISLEWPADMKKVDYVSQNTTHNRTSISILNDDDNEDDSLNDLHRPKFVPMAGPHSAPKQASKQRQKQKQSPHMMIENGDAAPLSAVSTQGLSKSQMGIFNQNENSLDYGMRSSRGFSHTVFENLKETPPAYEDLYDKWCEQWYQILRRTTAYFEDLKTEDRARDKVIKLFEFAGVKIVDTFDPDNTHFIVTQRPFVSKEAYPESDPFSYVHMKRIKVWHYEKCQRFFKSIRISNRRIDQLAKQRAIQKGIEVIDYLSGNGSTENNGNITNQNEDSFTVNEEEDSHTMQPSAQSKSFVNIAPAPTIDDHSNGKTSGNGKGKGRAKGKGRQKPISDTASAKENEPANSKKSNSKTAKPDEKNNKPESKTDDSKSKAAETKIENEQADKKSHKPVIVDDLLLPIKYDVSKPNWKVLSGLENLHFDFENCPITTSNVLEVWIFINMFHEAFVIDTFTFDDFLYALQWNDPSKKSPLLLEIFCALLSCIISKEGELLITLPVDIQSEIEEKEAEMKEKKLAKEREKKEKDKKSIKVEDDEDDEVENEDEDEENENENEGQSKENGNGTGVQVKEERKENAGAEKSFEVKEESEKDDDDDDDDDDINHNAYSILDYKKLSWRERLQKRHFKEGQWLIILMGIFSITEFIPEYKNEITKTYELLAPLDEVPTPETLMANFCEKVSPVRRVSMLSILMGLLLGGEVVRAHTDFVVSRSASLRRERFELQKELKLKVESAHIANKDVLDSLRTIDVREIKQRIEDRELEALTTEEEKEKWIEIKNRGGRPIQNTLPPEPSALEKAVAAGHPEFLKLLSARTEKISEVESMKKQRLEIDRTLVELNCQRIRYLGRDKVWNRYWWFEKNGLPNLGGNKDDDDENDEIDREERDDADNGDDDESGDLRKKDDNSEYDSETYLMGRIWIQGPSAIDVHHLRGGDGTGLKRKMIEEGENILKNERDWVFIDQVEDFNKLVSWLNDKGLRERALKKELNECRDRVISSFKARRNFLKGGESQIKLSNYIKKLEEGAVNEKETTKEETEKVEVMEIDSEDDRPAELDFEDEEAEEEEENGEDERAGDKDQKDHKKEEDEVLAIDGDSDSDTSRVTRSTRSLRNRKPLVAEEKLRRSKRNAAASPTSVNTRSKRRRVNTSDSSDDVSLDGSVDAGTGNDDENDDSTTVEQEFDDETETSKFNGKTLQSEEGYIDRMVRLLQLPGSDDPPELRTNLLIRARAKLGELDALNREENMILWVNSAAIEKNGYTHYEGPRIINSSRASRGNKKGRASKSSRGSRGRGRGRQSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFG7</accession>
    <name>A0A1E3NFG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF300-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_26507</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015949.1">
        <property type="nucleotide sequence ID" value="XM_019162450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFG7"/>
    <dbReference type="STRING" id="763406.A0A1E3NFG7"/>
    <dbReference type="GeneID" id="30179137"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044395">
        <property type="term" value="P:protein targeting to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41845" checksum="21752C9341AD31A3" modified="2017-01-18" version="1" fragment="single">MELERALPRWVIVICGVTTTSSILVSSLGIFLHLRNYRKPFEQRLTVRILVLVPLFSLSCYCMLMSYKVGRLIEPVREIYEAFTIYTFYKLLVLMLGGERRIILMTVDKPPTSHPFPASLFLKKINISDPQQFLTIKRCILQYVWFKPLLYLVIFITSVLGIYDVNDVSSSSIFVWIGVCYNLSVTTSLYSLAMFWKCLYDQLSAFKPWRKFMCVKLIIFASYWQGIIIGLLNWLGVFREREKVVLLNSSGNLGIQIQNGLLCFEMIFFAFLHWSSFPYTDFIVLKYPDAARIKPLVALKDCISIGDLIHDIKITTMYGDSYNLRNFDSIADSTVYNSSETFNQKIYQGLRISADGKKYWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFN2</accession>
    <name>A0A1E3NFN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_12698</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016060.1">
        <property type="nucleotide sequence ID" value="XM_019159963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFN2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFN2"/>
    <dbReference type="GeneID" id="30176650"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052247">
        <property type="entry name" value="Meiotic_Crossover_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835:SF3">
        <property type="entry name" value="HELICASE FOR MEIOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835">
        <property type="entry name" value="HFM1, ATP DEPENDENT DNA HELICASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="20"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="243"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1027"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1033"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1242" mass="139639" checksum="AE02FCC30F6A48D1" modified="2017-01-18" version="1">MFRSIFPFSQFNAMQSKSFDAIYNKNDNCVISSPTGSGKTVLFEIAIIKLLEARSNQSDDSLLNAKILYMAPTKALCNERYADWCSKFQPLNCAVGILTGDSPFVELDSIRKSDLIICTPEKWDVLTRRWADYSKLLNLVKLLLVDEIHILREKRGTSLEVVITRMMSMPLSLRIIALSATVPNINDVANWLGKSHDSEKSTTATTLVYDDTYRAVSLGKTVYGYQTMGTSNPFKFEGFLNGKLPEVIRLHSKGKPTLIFCSTRNSTVTTAKALLQGLNNPNTRLRNIPSGVSRDLLDLAQQGVAYHHAGLSWNERKFVEENFIIGSIKILCSTSTLAVGVNLPAYLVIIKGTKAWNNNSLEEYNELDLLQMMGRAGRPQFETKGVCVIMTDAFNKERYIRLAKGTENLESSLHLNLHENITAEITLKTINTLGTAFTWLKSTFFYQCYLHNPAAYPTVFKKFNSRLTPEAQLKIFLKRILSELEKEQMIEIADGELNSTSYGIAMSKSYVLYETMKQFIKYGEKLSLQDSLLMISESDEFKDIRVKLADKKLFKDINSSPLILNPIKDNKIEKFHDKVFLLIQFELGGVEYPMYQGSMKLHHEFISEKLLIFKSLPRILKAAVEVFSHRKDAISLASILKLTRCIYAKSWENSFLVLRQVDGIGLTYAKKLESRGITDIDQLKSLTGDKLEYYLGIKAGAGQKIAKSIASIPELSLRVENIKCESSGTVQFDVAVDLFNTEDNISYIWNGSFAHINVLTNVSGRVWDFRRLPLKKLVGGEKYNLKVDLGYKSDYIKVYLNCDEIAGLGKTVELDTFSATECLGTRPSSRKKGQSFDFDDDDEVLRAIFGNNSIEENSKKKLVDMGVTRTPQMEFADSSDASFSTVSTSTDSSFTECNHRCSDKSTCRHICCKHGIPKQKIKLCKHVCKDKSKCRHMCCRDQFDYETRRKSKIERKRMRQNTFDFAQCATPNKSFTSVVITENIIGKADDLSDGNPLNCEPTASNNKGVPLVKDCPTKMPVFLKKVSKPSPVRTEEPTSTKALKDDNSELGTESKYGKLKTSRFFKTSIRDSGMKRNRGLLDMSDSDEFSDTFDSALDKALKRHKRVKPIPKKDSSKKRDEAVFSKTTRAADMIILSNSDLPKLQKVYESGNSSQLDSAEDIIIVNQTLPKVIPVRKLYPLSSSHDSKNNQVTHYADHGGGDAEDTNTGCSGEDTTAMTSVMEEAVNKEIFGFLDSDIEFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFR3</accession>
    <name>A0A1E3NFR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="8">eIF-2A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_12681</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016039.1">
        <property type="nucleotide sequence ID" value="XM_019159956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFR3"/>
    <dbReference type="GeneID" id="30176643"/>
    <dbReference type="OrthoDB" id="2194683at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000596">
        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017222">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="10">
        <location>
            <begin position="238"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="462"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="639" mass="70302" checksum="582646F364882E4B" modified="2017-01-18" version="1">MSASIDSAKVPQFFCRQPKSVELIQAEPVFRSLPNFQTPEPTADTRCAQFIAGGKAFLYTLPSGVQVVDTVTGEVLVRIPIEDVFETNISPNGNYVTTWSKLTKDPAAGADAAPAVYLKNVNIINIDLPNRTFQILHSFLNKTQNGWIPQFTADESVVCFYRNPYSLNFFKLAGASTVFDFSKPLYTLDLKEFGKIEDFQLSPGKNPSVAVFIPGQKGNPAYIKVYSLPNVKSPVSQKQFFKGESCTFKWNALGTSILALVSTDVDSTNKSYYGETQLYLLDISGLFDQKIHLPNEGPIHEITWSPTSREFAVIYGYMPAVTTFFDSRGNSIHSLPKAPRNTILYSPHAKYILVAGFGNLPGDIDILDRQNKFSKVVSFQASNTSVCKWSPDGRFILTATTSPRLRVDNSVKIWYFNGSLVYYKAYTELNSVDWRFQPLTDFPPITVSKADKVDCEPDQSAIDYLNKQPKKNAANGGKVGAYRPPHARSSASSSGTGRSLADLHEQRVGGGRSFVPGMSPSASTTNVSDPAGSESKSAQKNRRKRANKKNENDSPVSSPAAPAPAALSTPATAESLVVGGVFSIEEKKIRNLLKKLRAIEALKQKQANNEHLEDTQVLKIQTEDAVRKELESLGWKGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFU4</accession>
    <name>A0A1E3NFU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonia transport outward protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_36252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016069.1">
        <property type="nucleotide sequence ID" value="XM_019162997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFU4"/>
    <dbReference type="STRING" id="763406.A0A1E3NFU4"/>
    <dbReference type="GeneID" id="30179684"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28419" checksum="BF68D10A05E222F7" modified="2017-01-18" version="1">MKASKIEESSDGNYVYLDNKKFVKSDLIKAFGGAMNPGWAVPSVHKFGNPAPLGLSAFAYCTFVSSLINIGTRGTHNDSVNTGAALFYGGFIQMVAGLWEFSLENAFGGLAFCSFGGYWMSAATLKIPWFNATASYATEHELNNAMGFFYLGWLMFTLLLVLCTVKSTVLFFLLFVLVFLRLMLLTIFRFYDLHSCEVGAGVIGVMAALLAWYHAYAGLATRQNSYYVVDPIAMPIISMNGLRRAAPKDSQEDDIELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFW3</accession>
    <name>A0A1E3NFW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_36079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016140.1">
        <property type="nucleotide sequence ID" value="XM_019162985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFW3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFW3"/>
    <dbReference type="GeneID" id="30179672"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF142">
        <property type="entry name" value="RAN-BINDING PROTEIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="4">
        <location>
            <begin position="116"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="254" mass="28200" checksum="16D6F16FAD0B205B" modified="2017-01-18" version="1">MFGSDNNVFSSSCKKTQAKVGKDLHNAERTVLGNDLSEAASTVVETDSKEHKNETSESGNETTDKDNNVTSKKAPVFGSGSTFGNAFQAAINKKSVFEELDSRDTGKKADDTTEKDVYKKVHLEKQDVKSGEENEETIFQVKAKLYHMELSKMSLGWKERGFGIIKVNRFIKSPAENYTSRLVMRQNGNLKLILNLPIMNGFQVLKGMPSSLTANKFIRLQVLEGGEPVQYAIKVGQPENAEKLFGSIRDQIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG65</accession>
    <name>A0A1E3NG65_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_166194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016215.1">
        <property type="nucleotide sequence ID" value="XM_019161280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG65"/>
    <dbReference type="GeneID" id="30177967"/>
    <dbReference type="OrthoDB" id="417678at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="214" mass="23611" checksum="85A39295A00AD969" modified="2017-01-18" version="1">MASEQQLTAEIKIEDSEVPHDHTGASINNNDLNDLDHEEPPLKRQKVKSEGPEATSNTMLPVPANSVTESELKQEKLPTNIETPKGSDGSRSNSTFVTQQTLPIATVQTTTAAGTTIEVVKHTLESKHTIPTQPSKADLVRQSGRSVEDIIDGSDLRKFLNKGLTEYIVKGLDEIVELWEKGKFNFDGQEPSKEVLKRKVALQFADIIKQLAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGA1</accession>
    <name>A0A1E3NGA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide hydrolase</fullName>
            <ecNumber evidence="5">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_35455</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="5"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016262.1">
        <property type="nucleotide sequence ID" value="XM_019162946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGA1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGA1"/>
    <dbReference type="GeneID" id="30179633"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000013">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="729"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="864"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="874"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="888" mass="99641" checksum="A3DCAC6C0A999860" modified="2017-01-18" version="1">MSLRRSSADLTARTGASGAGVANGGGNSAPAAIVANAGYGSGDAGNAGNEQNNNHAGNNSRRPSSIAESIVSQVVHLKDLTVSQLKRRRFFAVSIFSIICIFIFDLIFLPRTSLDRDLRRLYGGFLTFDDVSRIYISQLNHINDVEKYINIYNDQTHEPGGNYHIMENLMKNYEYLDTDVEKYDVYMGTPIGNSLKLLDEEGGLLFEANIRESDQISYFPFSKNGTVTSEFVYVNYGLAEDYDLLKSSSIDLTDKIFIIRINDTHPSLLVERAQKQKASGLVFYKDPYDDGEYTEKNGYMDFPAGYARNHHAIDKYTGSFIYYQPGDPTTPGWSPFLFEDHKRLKDPDTIPKIPILPISFTEIQPILRRLAKKGPSLNWVGDVLDFDYRPGPSDGLQLSIVNHIDYGIKPIYNIISTIRGIISDEEIIIGSARDVISGQGGVSQGGIGMLEIARGFNELARRNWKPLRTIKLISWDGSSLGQFGSTEFGEYHSQKLINNCIAYINFDSVRGNDLSIESNPLFNKVLKKVMELILLNSNETLGEAFFAKNSTLQLISNGVGDYSVFQNHLGIPSINIGMIPNKEDDPVTYFNSKFDGEQLLKSFDPDLKLHNLLAQFIGMLTIELSEHEILNVAVTDYIEIIKREVELTVDRLPAEWLDRNMTYPFEYTKLGDEIDILRMQSQDVCELAKEFDIELKDLQHLITQDFPWFKLYKKIKTAIKVKLYNTRVYFDITIGGAKQPRMVFDLFDKQVPKTAENFRALCTGEKGFGYKDSIFHRVIPGFMVQGGDITHFNGYGGKSIYGNKFEDENFDIKHTKPGQLSMANAGPNTNGSQFFITTVPCPWLDGHHVVFGELIEGGETLKTVENNGSPSGKPKAEMKVVDSGELKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGK1</accession>
    <name>A0A1E3NGK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_17733</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016355.1">
        <property type="nucleotide sequence ID" value="XM_019161717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGK1"/>
    <dbReference type="GeneID" id="30178404"/>
    <dbReference type="OrthoDB" id="415315at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990616">
        <property type="term" value="P:magnesium ion export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000095">
        <property type="entry name" value="Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="69"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="164"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="280"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="41190" checksum="A6D32A439257C38D" modified="2017-01-18" version="1">MFTPLKSTQAIPHSHPYPPSDSPTSSSPGSPSFPSGKNGKDTEDRRTSSNHDTNNNTNTTENKGTENIPSIWACALAGGLGGLIGDSSMHSLDTVKTRQQGASHINKYRGTWAAYGTIFREEGIFRGLYGGYSAAMLGSLPSSAVFFLSYEAIKRYEVNELGIPETPAFLAAGFLGDLFSSVFYVPSEVLKTRLQLQGRHNNAHFKSGYNYKGFIDAAATIIRTEGPGTLFYGYKATLTRDLPFSAFQFTFYENFRQWAFQLTGTHGKVDSFKQAETISLPLYAELLTGAAAGGLAGILTTPLDVVKTRMQTQNGTSGVILKSTSIISSLRTIYTVQGVSGLFSGVAPRFVWTSMQSSVMLLLYQVFLKAFSTGELDLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGK8</accession>
    <name>A0A1E3NGK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016367.1">
        <property type="nucleotide sequence ID" value="XM_019164652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGK8"/>
    <dbReference type="GeneID" id="30181339"/>
    <dbReference type="OrthoDB" id="3981234at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="393" mass="42853" checksum="DFFC249A7F854609" modified="2017-01-18" version="1">MAINTMPSYNQTPLYAPNVHVQAAKLPGYAVPTKAHEYAEYTPAFVQIPWQAQHAQQQAQRSHTPHYTPAEFHQSTHPVQQQQLFQNYLLNSTRASPVPAVPAAYIQHTHSLSHSHSPISAYSSSANSSFSSTATTPPLCSTTVFPIRKPAFQPLPPSSKPEELDRRQLQLKLKLKDPFVAPFPSRSSSAISTPVSAPSSACSATFSAGSLAGSAGIGKIVKPKNRSGLQKQTGSAKQFKRPTSASASSLVPAPTPTPSSAHAPLSQSAANKQSEISPDMYVPADPSLDISGFTEEDIIILKNLLSFAEIHKWKYISNKLSKTRSKKLNAEYCITKFHAMYGLPFSPKNSLLHSNYFLKIDKERKPEEESFEGILGSSIPYIVSKDGWNLIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGL2</accession>
    <name>A0A1E3NGL2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016403.1">
        <property type="nucleotide sequence ID" value="XM_019161736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGL2"/>
    <dbReference type="GeneID" id="30178423"/>
    <dbReference type="OrthoDB" id="10333078at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="355" mass="40462" checksum="FFC69153408A32BB" modified="2017-01-18" version="1">MSASISDAESDDWNVVSGEWRSYLKSEDGDIDDSIINKAIDDAQNNEDASDEGKEMQEGTKEPISNSKSGPELDSVGFNFDSLSNLKKVLSEKRSTFTILIESIVSLLFLYYSYTLVLDYFYKYKISNMMGSIEELPANAGYIELHTKRCNEAYFYYQDVLDSCLDSNTDDASCLVSYMKLVQKNAKFCSWDPEKIHKQQQLHVVYKHGREILERLTETSMKLVNTVGENVIEKGSQQWESLKSASQKVGPYYKMQLLRFSRKENELTSFLAKKNKDLDVKKNLENFVKGGEKVVVGTYESVRRNIPLIWDSAKDKTESGVQFISEYINRENAKKVYDGVFGACDNAIKNLLALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGQ1</accession>
    <name>A0A1E3NGQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_37281</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015636.1">
        <property type="nucleotide sequence ID" value="XM_019163072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGQ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGQ1"/>
    <dbReference type="GeneID" id="30179759"/>
    <dbReference type="OrthoDB" id="406833at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="4"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="154" mass="17338" checksum="BE60A34B2D6982A1" modified="2017-01-18" version="1">MSNLANKRVLKELKDFVSNNSATNGPLKLVSSQDKVDCILIEMRITSNPLYDPNTPFTLSYQIGDDYPFSPPQIVFVGSNIPVHPHIYSNGHICLNILYDGWTPVQSISSIVLSLQSMLLNNRVLEKPHDDENHCKYGPSNPLLSKWVFHDDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGQ2</accession>
    <name>A0A1E3NGQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_169290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016405.1">
        <property type="nucleotide sequence ID" value="XM_019161396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGQ2"/>
    <dbReference type="GeneID" id="30178083"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="136" mass="14963" checksum="93B0C5DF33D01131" modified="2017-01-18" version="1">MELRRGSTCCCQLRNGGRHGARGTGHSVLIEALVSALSASGLRALWQPLGGWQRRRGLLRWICFRRWGGPCHAGEPPECLALDGVSDLFCVCLVVRNCHILAFCATCAKPKRPRSASCFSFSSGVLAWMDPRVRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGV6</accession>
    <name>A0A1E3NGV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_35821</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016467.1">
        <property type="nucleotide sequence ID" value="XM_019162968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGV6"/>
    <dbReference type="STRING" id="763406.A0A1E3NGV6"/>
    <dbReference type="GeneID" id="30179655"/>
    <dbReference type="OrthoDB" id="414569at2759"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00362"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000001">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF1">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="18"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="189"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="429" mass="48751" checksum="3624587DD52880B2" modified="2017-01-18" version="1">MTIDISPASDDPSAICRDFDHVVFYVSSPKLVANYLCRTFGFSVYAYKGLETGSKDISATVIKNGNVHFVLMGAIRPLHKQPKDQMIDNIHKHIATHGDGVKDVAFQTDDINLIIKRLAEKDHSHLIVDGPNTITDEAGTIKTVTIRGMNDTVHTLVDRTQYKGFLPGFKIVEQEDALSGTSPKVDMAAIDHCVQNEDWNQMLKTCEFYKENFNFHVFWSVDENQIYTQYSALKSTVMSSENEHIKMPVNEPAIGLKKSQIEEFIEFNNGPGIQHIAIRVDNIIDTIKKMREMGVQFIDVPDEYYVNLEQKLKSSKHPKIKETLKSVKKLGILVDFDEKGYLLQLFTKPIFERPSFFFEIIQRNNYNGFGAGNFKGLFEVLEKDQEKRGNLTAQDVKDRTRGLPPKPKTTNFKSEITDDDIADAINYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGY4</accession>
    <name>A0A1E3NGY4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_172054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016523.1">
        <property type="nucleotide sequence ID" value="XM_019161508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGY4"/>
    <dbReference type="GeneID" id="30178195"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="83" mass="9272" checksum="CC3D24F18F31CBDB" modified="2017-01-18" version="1">MAEELSGGQNVQNESVVLCNKKFVPNAVTHTIPAAIKTALPKRDAGCVNRQHLVFIMYSKKLGRSYEEMTTFKRMPTDVGCRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH04</accession>
    <name>A0A1E3NH04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_13088</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016479.1">
        <property type="nucleotide sequence ID" value="XM_019160008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH04"/>
    <dbReference type="STRING" id="763406.A0A1E3NH04"/>
    <dbReference type="GeneID" id="30176695"/>
    <dbReference type="OrthoDB" id="3995949at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF2">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="282"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="362"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="473"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="508" mass="53465" checksum="7D3C928F558CD2D3" modified="2017-01-18" version="1">MSDIGGSQLTVQQLRVLGNEKFRALNYGAALMHYTQAIDQDPSSFAIYYNRAITLVKMDAMKEAKEDLLRSLEINPEYIPSLCQLGFIYLYEGNTPDSLETYVKVVKLNQELPHQLNRFKAQLKEAMRLAESRCKQQEYPQDFIDSIITPDIRESLDSYPNLPTHMIESGIPPVAFGNVPISHQFENASAVIARASIPIPVTGIRAATTTATNNSNNATTANDATISGNANNNTANENIANVLNIPGLLNALNTPGVSASVSIGGPQSSEELMNALGNFTNTQLSTEAPTGNNPVTGASSGPSTPNLSTSASATTSTTPTIIHAPVSRTTSGLPTEVLQRHREAHQAALNRAAELRRQREQYLGSSTTEQTPAENLATDSNVDSNVNSNDNISATNSTNSTATATTGSDFARQFASTIASQLTNAAVQNASQSGTSATPNSGAIQSLAQNLTSIASGVLGSLSSGNNFVSMVSNGNGSGNGSGSRNSSGNNGSSSESPLDADLDMDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH21</accession>
    <name>A0A1E3NH21_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycosyltransferase family 62 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74148</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016499.1">
        <property type="nucleotide sequence ID" value="XM_019164688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH21"/>
    <dbReference type="STRING" id="763406.A0A1E3NH21"/>
    <dbReference type="GeneID" id="30181375"/>
    <dbReference type="OrthoDB" id="2405412at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140497">
        <property type="term" value="C:mannan polymerase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="43224" checksum="DD5EA29A4658FB5C" modified="2017-01-18" version="1">MSLAQYKRTVHRLLTRSNPLKLVVTVVLVLFLYFLVRGTGPREEESVIAANRKKSPYSYKPKAGGKNAWTPKNLPADHISHYDLNTLKASEDALQNNEKVLILTPMANFHEDYWQNLLELSYPRELIELGFILPRTAEGDAALKSLETAVKKVQGAKDASASKFSKITILRQDNDSPVSQLEKDRHALKVQKERRSQMATARNSLLFSTIGPFTSWVLWLDADIVETPRSLIQDMTSHNKPVLAANCYQRYFDQNENKESIRAYDFNNWAESDEGLRLASTMADDEIIVEGYQEIVTYRPLMAHFYDETGNVNDEMPLDGVGGTCLLVKADVHRDGAMFPNFPFYHLIETEGFSKMCKRLGYEVFGLPNYLVFHYDQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH22</accession>
    <name>A0A1E3NH22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">XPG-I domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016535.1">
        <property type="nucleotide sequence ID" value="XM_019160020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH22"/>
    <dbReference type="STRING" id="763406.A0A1E3NH22"/>
    <dbReference type="GeneID" id="30176707"/>
    <dbReference type="OrthoDB" id="2959108at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09870">
        <property type="entry name" value="PIN_YEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF72">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE YEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG-I" evidence="2">
        <location>
            <begin position="149"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="734"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="983" mass="111309" checksum="2B5B3D7052F54C37" modified="2017-01-18" version="1">MTIDGFWKEVNEKIPLEQYRVSFPDYIYSHYSRRRKFLTVGIDIFQWIFEHASFAESQSYNGFPESEKSVAVVIAGIASKLRYLISLPLNFVIVFDGKIKFDKHRWDIENESSNDIIFEERYKDDLDRIRKDEHIMNGRCITSLLELLDAWNISYVHAPGDAEIELARLNKAGIIDAIISNDADGFAYGASVILRNFSKFSNDKPSTATKGGIRKGNDYFVTPVDAELMKQLNLDTDKIIFIACCQGDDYSKGVKGLGIKKAWALATNKNADFDAVQGLKDIYVSKTNDEYIKGNMPYPYRKRLVLLSEYKTKLMEHIADNGKAYFGRVHSSDVVLPDDYVISSHFYPHFSKYLFEFESFETNFSEYSGLLNNKCYFPSLPQPLSIAKSIGNRYEVYRGNSLKGFMCFTVFKEQIGLTSERVKYTSFHWFQSLNYGSLLKWTKTGRSQREGMEFLAESLGRCYLWRAIVNTKELNLKAGDIFIKNQKSVQINCEGREFEQAFYQVKFKPREIFKSILGLEENMVDVNLNDIEEEHIHVWLSSYLFFVDDNGRKLVEIYNDEKTNKANTPKTTPKRTPRKNRTPLQKTTLDLLSGSSKSPIKILTLKPGLLEISEASKSVTVKRSVLDVDTSNILSSPKKKIKHIIIDDDEPAPNPILLEPFEEDAEISRVLENSDYVGGNSFIDNNSNINDNDSNKDSNMNEDIFFDSHENLNSFMVESHQPGVERSEQKDNIIDSGGKDIYTSSPGPFNKSTGLIPARKELKKDKLTIHRGVSNLDEMTRLPELPLGPENRGSHEDEGGDPLLGIHCEILDDTFSSTDSIEKLFAKENKNDGDVSNKVTAVCGQAEKITLDDTSSFTDTIGGIFGLQHHATHETHGTPEEMPATVSIDLTPNYRSENNSDVETLEEATSTPVKKLQASGPVKSGSLLQNALHQEHQQENSYSLPIGFDGKEILLAESTDDESMWVVPEKNQPSVFDEDILEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHC2</accession>
    <name>A0A1E3NHC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_34463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016635.1">
        <property type="nucleotide sequence ID" value="XM_019162887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHC2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHC2"/>
    <dbReference type="GeneID" id="30179574"/>
    <dbReference type="OrthoDB" id="2348401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2440">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="64" mass="6968" checksum="33AD16D148ECB7C2" modified="2017-01-18" version="1">MSDSARKNFSDKVSDAVTPDSQKSGWDKATEGVSNQYDKAASKVQPEEEKGVFQKISDTISGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHJ2</accession>
    <name>A0A1E3NHJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_22184</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016728.1">
        <property type="nucleotide sequence ID" value="XM_019162239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHJ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHJ2"/>
    <dbReference type="GeneID" id="30178926"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="160" mass="17484" checksum="F342C2B90135D0FE" modified="2017-01-18" version="1" fragment="single">MAIYSLYLISKSGSLLYQRDFKTAGSPITKQKSNDYLIIASNLHGIHAIASKLTPAAARGSLSGNTGGGRKNTGGGINVHSNKTGLRCVNTEKFNMFVHQTVTGVKVIILTSTDLSEPQIAGFQTTIHRLYADYVMKNPFYSLDMPIRIKLFDDRIMSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI02</accession>
    <name>A0A1E3NI02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_35384</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016883.1">
        <property type="nucleotide sequence ID" value="XM_019162940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI02"/>
    <dbReference type="STRING" id="763406.A0A1E3NI02"/>
    <dbReference type="GeneID" id="30179627"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="78"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="287"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="381"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="479" mass="53816" checksum="9EF500BC0D67E098" modified="2017-01-18" version="1">MAKQLGIEGEMENLSGDDYESEGEIIELDQSDEELENAADVDGLIGKVQQSGPNEGDASKEEENALYLPHRSRPLGPDEVLEADPTVYEMLHNVNMPWPCLTLDILPDRYGDERRSYPAKMYVATATQASKAKDNEMIIMKLSSLSKTQAKSEDDENDSDLDDDDDEQYSSVLESESIPLKHTTNRLRVSPWASKTNEYLTATMSESGEVYVFDVSSQFKAFDTPGYVIPKQSKRPVHIVKNHGNVEGYGLDWSSTEEGSLLSGDITGRVYYTTRNNSKWVTDRTPYQVNDSSIEDIQWSKSEKTVFATAGVDGYVRIWDTRSKKHKPALSVVASKSDVNVISWCDRIDYLLASGHDDGTWGVWDLRNFQPNTQPSPVVSYDFHKSPITSIAFNPLDESIIAVSSEDNTVTLWDLAVEADDEEIAQQRQEMKELSDIPPQLLFVHWQKDVKDVRWHKQIPGTLVSTGTDGLNIWKTISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI46</accession>
    <name>A0A1E3NI46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_17729</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016351.1">
        <property type="nucleotide sequence ID" value="XM_019161712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI46"/>
    <dbReference type="STRING" id="763406.A0A1E3NI46"/>
    <dbReference type="GeneID" id="30178399"/>
    <dbReference type="OrthoDB" id="5376590at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000005">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 64 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF50">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="559"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="712" mass="79671" checksum="DF16DA233CB17503" modified="2017-01-18" version="1">MIFSRPIRLLGINSTSATRRYSSQRYRGFAKPGVLLSALRAAGPAGLHSGRKPFSFSAAACSGGSTQPSNSEPPHVPAKIVPPPEKKHESSPGRYSFVKKSFRFFIVGSGVISGSLLLVLGSFFIYDYTTYSPPEVPQNLLIPFDSLNPPIGGPENLPILVKNLDSYDSASKISDMQKKKLVILGCGWGAVSLLRQLSKDEYDVTVISPTNYFLFTPMLPCAAVGTLDIKTLMESIRSLINNINGHYLHAKAERVEFSEKLVKVQDMETSKYFYVPYDKLVVAVGCTSNNHGVKGLEYTHRLKTAEDALTIRRKLLRNFEQACLPTTSDAERKKLLSFVVCGAGPTGVEVAAEIFDLINEDLPRLYPKILRQEVSIHIIQSRSHILNTYDKKISEYAMDRFSNDSIDLLVNSRVNEILPDKVIFNQKKENGEIEQREIPYGLCLWSTGIGLNDLTKQLSDDLKPFQRNKRALETDSHLRVIGAPYGDIYAIGDCSTVRTDIGDHCEDYVRQFILNHKSIRERDESKLITDADIKNFEITHSQLKGLCSEIAKHNPLALEALLLAEELVCKYDKENTGKLKFKDLKMLFKEVDSKVTALPATAQRAHQEGNYLGKKLSKLALAEENFLQHDIHNGDIDDAVSKPFKYKHLGSLAYLGNSAVFDLPGHSLFGGLVAMYLWRSVYFAQSVSIRIRVLMFFDWVKRGIFGRDTITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI91</accession>
    <name>A0A1E3NI91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016965.1">
        <property type="nucleotide sequence ID" value="XM_019161472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI91"/>
    <dbReference type="GeneID" id="30178159"/>
    <dbReference type="OrthoDB" id="3996873at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19311" checksum="F786F9DEABE1C373" modified="2017-01-18" version="1" fragment="single">MPPKKNNADLLLLKLKRQASTYLVECNVNDTLGELKQKLTKMINSTNGIKVNDEPISLDVRDSIIMQADTKRVPMPKIGLNDSDSDADAKGDEDIDIDDGKSTATEKNDASNVRVSVDAIKIGNFENSNDIYASKITEQSEPDETKLKGLGLQDFVALAFKLEGEEFRIYKPQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIB4</accession>
    <name>A0A1E3NIB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxidase</fullName>
            <ecNumber evidence="14">1.11.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_34873</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 Fe(II)-[cytochrome c] + H2O2 + 2 H(+) = 2 Fe(III)-[cytochrome c] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.11.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms a one-to-one complex with cytochrome c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Cytochrome c peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.-" evidence="14"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016993.1">
        <property type="nucleotide sequence ID" value="XM_019162911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIB4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIB4"/>
    <dbReference type="GeneID" id="30179598"/>
    <dbReference type="OrthoDB" id="2859658at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004130">
        <property type="term" value="F:cytochrome-c peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000009">
        <property type="entry name" value="Ascorbate peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF58">
        <property type="entry name" value="CYTOCHROME C PEROXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 14" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="16">
        <location>
            <begin position="107"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="365" mass="40639" checksum="B6D1CAA1436BC1D7" modified="2017-01-18" version="1">MSSASAYANRSLSAARQSARYRFRLSSPLLVSLAVAGSAAYAYNSNNNNGNGNGSFVSKALFGFGASSKVATNVREASAKHDQAYYQKVYNAIAQKLVDVDDYDYGSYAPLLVRLAWHNCGSYDQDDNSPTKGGSYAGTMRFKKEQGDPENSGLVLGMRYLDSIKDQFPDISHGDLYTLGGVVGVQEMGGPKIAWRPGRVDLPIDVTPPYHRLPDASQTTGSYVRSVFTDRLGFTDQELVVLIGVGHAIGRCHTKNSGFDGPWTFSPTTISNEFFKLLLSEDWQFKKWDGKKQYEDQGSHSLMMLPTDMVLKQDSKMREYVELYAKDEDRCMKDFADAFSKLLERGIKFDSKKMYFKTLDEQGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIF9</accession>
    <name>A0A1E3NIF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 8, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome c oxidase polypeptide VIII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_17839</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cytochrome c oxidase VIIc family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016481.1">
        <property type="nucleotide sequence ID" value="XM_019161769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NIF9"/>
    <dbReference type="GeneID" id="30178456"/>
    <dbReference type="OrthoDB" id="9974841at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.49.10">
        <property type="entry name" value="Cytochrome c oxidase subunit VIIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004202">
        <property type="entry name" value="COX7C/Cox8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036636">
        <property type="entry name" value="COX7C/Cox8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT VIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02935">
        <property type="entry name" value="COX7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81427">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="74" mass="8385" checksum="AA0947F8BEAAD7BB" modified="2017-01-18" version="1">MLARAFQRQAVRQFSASTKKLSGPHFPEGAYNNLPFKVKNRKVPYAIPHLIFWILGGGIPFFAVTVQQKRAGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIP1</accession>
    <name>A0A1E3NIP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Yos1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_148512</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the YOS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017104.1">
        <property type="nucleotide sequence ID" value="XM_019160565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NIP1"/>
    <dbReference type="GeneID" id="30177252"/>
    <dbReference type="OrthoDB" id="15356at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013880">
        <property type="entry name" value="Yos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858:SF0">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08571">
        <property type="entry name" value="Yos1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10909" checksum="EB1099AB5270D6F8" modified="2017-01-18" version="1">MLGTLVYAVLFFINGIAVLSEDRFLARVGWSAKSSSSNINGMPQQQYGYGDFNSMSSGLPSSGGEGIKSRLINLISATRTLLRIPLIIVNILVIVYELLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIW5</accession>
    <name>A0A1E3NIW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_12516</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017192.1">
        <property type="nucleotide sequence ID" value="XM_019159938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIW5"/>
    <dbReference type="STRING" id="763406.A0A1E3NIW5"/>
    <dbReference type="GeneID" id="30176625"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF13">
        <property type="entry name" value="SUBTILASE-TYPE PROTEINASE RRT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133377" description="Peptidase S8/S53 domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="9">
        <location>
            <begin position="31"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="141"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="45071" checksum="4D305D5A28E30F46" modified="2017-01-18" version="1">MRFGLSLVLFVCLLSYVHSFPLFNFNKKERYIIQLRKPDSVEVLCAQDDAVKSFSHLRREIKKVFSFGKFEGFVGEFSKDVIDRISQNPLVEKITQDFSIQALDEIAVQEDAPSHLVRLSQDEPVDEDEEMNYYYSEDFQGKDITVYVIDTGVYVEHPEFEGRAKLGPNFSTDLRNIDYVGHGTHVAGVLGSKTYGVAKEVNIHSVKVLDRFGQGSLSSVIAGLEYSVNHQKENGLHAVANLSLGAAKSSVLDAAVQAVYDSGLLVIVAAGNNNMNACRTSPAGSSYAFTVGAIDDKTDEIAHFSNWGNCVDIFASGVEVKSLNSNRHGSDIQILSGTSMASPIAAGLAAILIEQGIAHGQVFNEMRNLANLNSIPATSLRLRPGSPNAIANNGVIANSAAVSKINNTIANVQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIX8</accession>
    <name>A0A1E3NIX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan-branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycogen-branching enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_73571</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017210.1">
        <property type="nucleotide sequence ID" value="XM_019164519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIX8"/>
    <dbReference type="GeneID" id="30181206"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000001">
        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000003">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="12">
        <location>
            <begin position="168"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="697" mass="79549" checksum="E4C46A90099AAB87" modified="2017-01-18" version="1">MAEHTKVPASMKELTQVDPWLEPFVEELAYRSSLANQWIEKFNANEGGLLAFADSYKTMGLHVQKDNSIVYNEYAPNATHACLIGDFNNWAHDANVMTKSATGHFTVTVPPNADGSPGIPHNSRVKIFLTLPNGEKIARLPAYIQRATQPPKEYNNPSYEARFWNPPQKYTFQNKRPKLPASLKIYEAHVGISTPEPKVGTYNEFTENVLPIIKDLGYNTIQLMSVMEHAYYASFGYQITSFFAISSRFGTPEDLKRLIDTAHGMGLRVLLDVVHSHASKNVEDGLNMFDGTDYCYFHSGGKGVHDQWDSRLFNYGHYETLRFLLSNLKFYLDEYGFDGFRFDGVTSMLYLHHGVGAGFSGDYNEYLSPFAPVDKEALAYMMIANDLGSLYSAANDCTITTIAEDVSGYPTLCRPREHGGVGFDYRLAMSIPDMWIKILKHKKDEEWDMGDITHTLSNRRYREKCVAYCESHDQALVGDKTLAFWLMDAEMYTHMSVLSPLTQVIDRGIQLHKMIRLITQSMGGEAYLNFEGNEFGHPEWLDFPRIGNGESYHYARRQFNLIKDDLLRYQFLYQFDKAMNETETRFPWLNTEPAYISLKNESDKVVVYERANKLFLFNFHPTNSYVSYRVGVETPGTYKIVLNTDRIEYGGHGLIDEGKSTFFTTDLSWNGRRNFVEVYLPARTGLVLALESDIARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ50</accession>
    <name>A0A1E3NJ50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit G</fullName>
            <shortName evidence="5">eIF3g</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 RNA-binding subunit</fullName>
            <shortName evidence="5">eIF-3 RNA-binding subunit</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Translation initiation factor eIF3 p33 subunit homolog</fullName>
            <shortName evidence="5">eIF3 p33 homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF35</name>
        <name evidence="11" type="ORF">PICMEDRAFT_35014</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit G family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017209.1">
        <property type="nucleotide sequence ID" value="XM_019162916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ50"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ50"/>
    <dbReference type="GeneID" id="30179603"/>
    <dbReference type="OrthoDB" id="639027at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03006">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017334">
        <property type="entry name" value="eIF3_g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024675">
        <property type="entry name" value="eIF3g_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12353">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037949">
        <property type="entry name" value="Transl_init_eIF-3_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="10">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="186"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="265" mass="29132" checksum="9C1DCEF6C1ED1454" modified="2017-01-18" version="1">MASTNWADAEAELAPPQVIDNNDGTKTVISYRFNDQKKKVKVTQKIKFVKTMEKVNPLVAQRAHWKRFGVEKDNKTVGPDSKTTQITEEVKLILSTTWKADEDREQEQAKNKQVKSTFKCRVCGGTGHYTVKCPYRDTFGSDKMFNPSGAADADAKAGTPAPGSSRYVPPNMRAGGSDLGVGAEVPALRITNLNAAIDRDSLQMIVSKFGNYDRVSILKNRETGESLGVAFVNMTTQRGAEAVKEALDGRGLMNMIISVDWARPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJB0</accession>
    <name>A0A1E3NJB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_67767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017347.1">
        <property type="nucleotide sequence ID" value="XM_019163501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJB0"/>
    <dbReference type="GeneID" id="30180188"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133387" description="Secreted protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9757" checksum="225880E749414DD0" modified="2017-01-18" version="1">MDGWMISGGKTWPRGSTLGCFWALGACLPAFTFIEEASCRRSASCFIVAGSRRKQNATAWFPCGDKKNARFQKRRTAQGQLAPEPLLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJH9</accession>
    <name>A0A1E3NJH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative tyrosine-protein phosphatase OCA1</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_73242</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative tyrosine-protein phosphatase required for protection against superoxide stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016841.1">
        <property type="nucleotide sequence ID" value="XM_019164422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJH9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJH9"/>
    <dbReference type="GeneID" id="30181109"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14531">
        <property type="entry name" value="PFA-DSP_Oca1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF8">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="77"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="238" mass="26819" checksum="C14E23C4E67984FD" modified="2017-01-18" version="1">MSVLENQVGLDPSEKKDKKEKKDKKEKKDKKDKDVPPADAVLITDLPSDVIKTHGDAGDGVRIITDAPKFRVVPPLNFCPVEKHLYRSGQPSALNHSFLEQLNLKSIIWLAIEDPQDSFLKFIDENNINFFYNLGYDSIGSNSWDGLSEASIKQALEIICDRRNHPLLVCCGMGRHRTGTVIGCLRKLQGWNLASVGEEYRRFTGVKGGRILVELLIEAFDVHTIEINPQVAPEWLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJL0</accession>
    <name>A0A1E3NJL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Conserved oligomeric Golgi complex subunit 5 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016646.1">
        <property type="nucleotide sequence ID" value="XM_019164367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJL0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJL0"/>
    <dbReference type="GeneID" id="30181054"/>
    <dbReference type="OrthoDB" id="18786at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019465">
        <property type="entry name" value="Cog5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049176">
        <property type="entry name" value="COG5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228:SF3">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10392">
        <property type="entry name" value="COG5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 5 N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="418" mass="47842" checksum="6AF5AAFEABCE27F6" modified="2017-01-18" version="1">MESSNLSEYGDYLSKSFNAGELANKLLLETNNAQDSDIELETSIKRLDFDISDLNSKIDDNVRENSGLLIEEFAQVEKFKDEIKVIQPSVGQLNNSFQRLENEIIKPYNECVNLQMALKKVHQTNKLLRSLTFAIYLINKIEEIDKSENNLSVKPFKHLYSLSVLLRELTSYISNPSLKLIKLVRDYVQFSEILIKRCQNVIQVQTRNLLKFPIQEYVTNTGGAEPEQDTEKSLFNLLSSKLLLDEKNLVSSIELIYTASSKHSINLILRNLNNTKYLPSYINSLERPSRLIAQLERCIKSMKWVDEFGSGNTEVSVWDHLLSTNASLILGGDEERQSRGLLDRYWREIALGVDSGVREVVNRGGPIVRNLKNIKGELEKAIGEVVSGSYSEMGEPFKVDKLEYRMMLNSITNFERRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJU7</accession>
    <name>A0A1E3NJU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_129217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017512.1">
        <property type="nucleotide sequence ID" value="XM_019159988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJU7"/>
    <dbReference type="GeneID" id="30176675"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="316" mass="32789" checksum="ED0A42ED97B165BF" modified="2017-01-18" version="1">MCGLWQGNASVARGAGASWLACTRDTRPPPATSSSTTQRATFHLHNHLPVQKTVCLRPAFAVPRGLPGTIARGRLGPGRGGNCGDKPLSAFRPPSCPPSPGARAFSTPHPCTGYMPPHPCTCNWPFFLPVEAHRFPSAAFARLGALPPAASTPPLSATSLSCPVCSIFAAPTLWGRGDVSSARQASRAVGAAGWRHWVLRSGGASLWLGSGSTQTDPAPLVLAFCRTLGPFVLVQDLALAHTPSLPVFSVGVSPSSPVVTQTIEKIVAAAPAHHHSSDKLPGFTCTLIPTSGGTPLLPCVAPRTPPCVVIMFACQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJV5</accession>
    <name>A0A1E3NJV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ran-specific GTPase-activating protein 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017537.1">
        <property type="nucleotide sequence ID" value="XM_019161159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJV5"/>
    <dbReference type="GeneID" id="30177846"/>
    <dbReference type="OrthoDB" id="512915at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008812">
        <property type="entry name" value="Ran_GTP-bd-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010:SF2">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05508">
        <property type="entry name" value="Ran-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="49854" checksum="BBAC9A93A20639F5" modified="2017-01-18" version="1">MDQLLSKAGSTLVTFAVRSGVQVASTYVIKSVSTLMDHVPEQQKKKLDRKRNQLESKVETVTYSIEVLRLMAAKGNSNLSYVLKLADYLKEDIDNFSKDIEILTADLKKKKISSELLKIIEKTIDDLVEKIDNIIPILNLVLTTYGTSTVNNFQDYVSPGRLLNSTVLVNQSNEEFVKHKSGQEINVGPEFSLTFYNIYYNHTAASNGEHQITWREKYARCKFQVVRVPNNEVEYCYKLKILEDFDDERYHEEDEKPERKSYDIRQISKLFFSASGRLLKLEDRSSPVLVLKTMKTTSEELDSETLNGAEEKPHDDGGVEWIAVGDYEMGTTESDSDDDDEEEDEHDEEKSFTEAKQEIEAKATDKAKPNNKIGNSSPLSLLEYLIRLCTLQANDQMPLLEVKDERLRLYLADENQMNKTPNKVASLNKKMESLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJZ9</accession>
    <name>A0A1E3NJZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16332</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017554.1">
        <property type="nucleotide sequence ID" value="XM_019161170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJZ9"/>
    <dbReference type="GeneID" id="30177857"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="276" mass="30199" checksum="EAD5546E9D5E2B72" modified="2017-01-18" version="1">MSDDHSTNTAVPENGVPQKEESPVVPEQNVNTAYPSLYEGRPYNYDGQVHPPRTGLLRTSWYKRTGDHRPFPTSVAAGLACVATTLFGLGLIIAGARHTPSSAALTGSLFFSSGLVQVITGIWAIVDNNLFGSVFLLCYGAFFMSLGSILAPIFDIATAYADGGLTDALGIFLASWCVFTFFLWTATWKSTVPLFVLMFCLWMFFLLYTIAVFGNHKGCQTAAGVFCFLTSACAYYALYDALSEPYNSYGSLPEAKILRMPGSWNVPDDEKRIEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK02</accession>
    <name>A0A1E3NK02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="7">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72517</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="7"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017562.1">
        <property type="nucleotide sequence ID" value="XM_019164217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK02"/>
    <dbReference type="STRING" id="763406.A0A1E3NK02"/>
    <dbReference type="GeneID" id="30180904"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="8">
        <location>
            <begin position="29"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="430" mass="47796" checksum="44C99ED08B16BCC5" modified="2017-01-18" version="1">MSVRDVSLSYEAILHQAGPFTEPDYDYDAGKAELESSHVLVIGAGGLGCEILKDLALSGVKKISVIDMDTIELTNLNRQFLFREENVGKSKAIVASDVVVQRMQNGIEITPYFAKIQDFDDDFYSQFSIVVCGLDSIEARRWINAELVHLALDKEKVIPMIDGGTEGFQGSVKLIVPTFTACFECYMPLIPEKTTYPLCTLASTPRLPEHCIEWAHQLEWEKVNPEVAFDADDMGHVEQMYKLARARAEQFGIEGVTKTKTLGVVKNIIPAIASTNAVISAQCCNEAFKFLTSCNPSMKDTMFYNGENGVLCETDEYLPLPSCPVCGRRVEELVLPAADAAITLEQLALRIKDRYTLQKPVLMVDGQEVYNFKYVEDSLEKNDLPAVDVFSESEVTITVVDTSVENTLEVTVTFPLPEKKGEEKKVTNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKP2</accession>
    <name>A0A1E3NKP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_134060</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017803.1">
        <property type="nucleotide sequence ID" value="XM_019160055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKP2"/>
    <dbReference type="GeneID" id="30176742"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019603">
        <property type="entry name" value="Tom5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10642">
        <property type="entry name" value="Tom5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="56" mass="6312" checksum="7E0CEA8F59DD333A" modified="2017-01-18" version="1">MNGYSEEDIRNAMLYQEQMKKAQEEQTALTLKLAGLLAFGLWAIPTAIHFVKKSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKP4</accession>
    <name>A0A1E3NKP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinetochore-associated protein MTW1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_16525</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mis12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017802.1">
        <property type="nucleotide sequence ID" value="XM_019161241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKP4"/>
    <dbReference type="GeneID" id="30177928"/>
    <dbReference type="OrthoDB" id="1884855at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008685">
        <property type="entry name" value="Centromere_Mis12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527:SF2">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05859">
        <property type="entry name" value="Mis12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="36346" checksum="481CFDDDDA2916C0" modified="2017-01-18" version="1">MNEEEIQLNTTRLLTEHFGFQPITVIDDIINVINEIMYRCTEQLEQILINQKLQLEESANERLLQQRSDDIIVDANLSKTREEKQYSVEDIQTGTATLESLLEHSINRNFDKFEVYALRNVFTLPEDLVSGGHVRLKHHEGLQVYDNIVQKDREITQEMLETVRQIQQQVEFNKVLCDAVPKFQKLARVSKLVKMRLQPLIADEQSGPETRRTLAQLTPLNDTIFFLVTQLRSTCDKVNAMKDLISNDKLAERFRHGSKEEQQLNQRINVIIEKVTQGVTGKRAADPARTTEGPLVPVDSQSGQEIIDFFATDSAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL63</accession>
    <name>A0A1E3NL63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase ESA1</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Protein 2-hydroxyisobutyryltransferase ESA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Protein acetyltransferase ESA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein crotonyltransferase ESA1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PICMEDRAFT_72917</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalytic component of the NuA4 histone acetyltransferase (HAT) complex which is involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, and histone H2A to form H2AK4ac and H2AK7ac. The NuA4 complex is involved in the DNA damage response and is required for chromosome segregation. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) through homologous recombination. Recruitment to promoters depends on H3K4me. Also acetylates non-histone proteins. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA) or (2E)-butenoyl-CoA (crotonyl-CoA), and is able to mediate protein 2-hydroxyisobutyrylation and crotonylation, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2E)-butenoyl-CoA + L-lysyl-[protein] = N(6)-(2E)-butenoyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:53909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2-hydroxyisobutanoyl-CoA + L-lysyl-[protein] = N(6)-(2-hydroxyisobutanoyl)-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:131780"/>
            <dbReference type="ChEBI" id="CHEBI:144968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:24181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:45949"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017998.1">
        <property type="nucleotide sequence ID" value="XM_019164330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL63"/>
    <dbReference type="STRING" id="763406.A0A1E3NL63"/>
    <dbReference type="GeneID" id="30181017"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044016">
        <property type="term" value="F:histone H3K4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106226">
        <property type="term" value="F:peptide 2-hydroxyisobutyryltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140065">
        <property type="term" value="F:peptide butyryltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140064">
        <property type="term" value="F:peptide crotonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140861">
        <property type="term" value="P:DNA repair-dependent chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031453">
        <property type="term" value="P:positive regulation of heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000022">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF218">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE ESA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="24">
        <location>
            <begin position="176"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="22">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ODQ46885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="460" mass="53903" checksum="7D490DD2B23FF55D" modified="2017-01-18" version="1">MTSDSEQTLDTFDDAALAPPERAESPLFKTNNIIPGSKVFVLKDERYRLAEILAFQVRKSRPNYYVHYVEFNKRLDEWIDEDAIDFYRPLTLPKKQNNKKDHKKQKKKTAGQKSNTPDVDQKMVIKKEEKDEGVMDLDNLNVQGLVREGEELTREEEIKKLRTSGSMTQTNVSALAKIRNFNKVIIGKYEVEPWYFSPYPMEYTEEGSLYICDFSLNFYASKKQFERFRSKCTLKHPPGNEIYRDDYVSFFEIDGRNQRTWCRNLCLLCKLFLDHKTLYFDVDPFLFYCMTRRDELGHHLVGFFSKEKECAEGYNVACILTLPQYQRHGYGKLLIQFSYELSKKEGKVGSPEKPLSDLGLLSYRAFWAETIAELLIENKPAEISVEEISQQTSLTTTDILHTLQTMNMLRYYKGQHIICITDQVSQAHDKLKKKNRHKLDPNKLIWKPPVFTASQLRFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLB6</accession>
    <name>A0A1E3NLB6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_33784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018023.1">
        <property type="nucleotide sequence ID" value="XM_019162845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLB6"/>
    <dbReference type="STRING" id="763406.A0A1E3NLB6"/>
    <dbReference type="GeneID" id="30179532"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013945">
        <property type="entry name" value="Pkr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251:SF1">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08636">
        <property type="entry name" value="Pkr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="108" mass="11985" checksum="72D8998CD0342CAA" modified="2017-01-18" version="1">MSSFFVDLWESVFTPGTNPALIRATHSSFVMLVTSLVWMVYTSRSIHFFNLLVIALCLWASVTWFLSELAKEKLKSNEQLLKEAGGDAAAGGADQKEKAAGEEKKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLE4</accession>
    <name>A0A1E3NLE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_139397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018053.1">
        <property type="nucleotide sequence ID" value="XM_019160144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLE4"/>
    <dbReference type="GeneID" id="30176831"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="146" mass="16026" checksum="058490EE564FFB97" modified="2017-01-18" version="1">MVGYNERYDGLDRQGDKKKQKKKITATATVPTLATTTTMAGESGGIEQLFSVCWGRSEIGFDEFAELILGVTERCPDTDLRVIEKMLEFEDEAWETGCGGLDYSIDELRRTVLVEGITGGTRDVADIQGVLGGLLARVGEMSRQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLG9</accession>
    <name>A0A1E3NLG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018087.1">
        <property type="nucleotide sequence ID" value="XM_019164355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLG9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLG9"/>
    <dbReference type="GeneID" id="30181042"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF8">
        <property type="entry name" value="INOSITOLPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="253" mass="28227" checksum="68E8C547CD5B3A51" modified="2017-01-18" version="1">MGYAAGLVRIDSSLGTHLATNGFHKSPIVFGALPSLHSAMAVLSCMYISWFTTSKFLVVLSFTFVMVQWWATIYLDHHWRLDLFAGMCYATLAFLTFRSFNPQRFNTTHIYTSTAIPTPAGASTHSNTHPSYTSYTSVENRDLELDSDEFDLDCDLELQCLSKAIPTSPKKPTDNINGEAPDHYQCQLEDEEHKLDYRYCEYEDSNSSNSSSSSSEISSATASSNASVSLLVQEDRPFGLRLFAGTPLEFLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLH6</accession>
    <name>A0A1E3NLH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_16781</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018097.1">
        <property type="nucleotide sequence ID" value="XM_019161340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLH6"/>
    <dbReference type="STRING" id="763406.A0A1E3NLH6"/>
    <dbReference type="GeneID" id="30178027"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000287">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="20">
        <location>
            <begin position="126"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="19">
        <location>
            <begin position="467"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ46984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="733" mass="81170" checksum="A24B579B74CB7085" modified="2017-01-18" version="1">MWFRRVLVVRSPGLFPQQNHNHLRRYSSLLRQSVCSARSLSSRYAWGVSRNPLPLHSQKKSVLGSGAAKRYNSSSTPKKPNLVTSSKQAKAGPSLPNVSQLKIIANLMAYLWPKDKPWVKFRVVSALSLLIAAKLLNVQVPFFFKSIIDQMNIDWQSLSDSIAKTLPDSLVTQSIIDNAPALPSAIVALIIAYGVSRFGAVLFGELRNAIFASVAQGTIKQVARNTFVHLLNMDLSYHLARQTGGLTRAIERGTKGISYVLSAMVFHIIPITFEISVVCGILTYNYGAAYAAVTLLTMMAYSIFTIRTTTWRTGFRRQANKADQQAASVALDSLINYEAVKIFNNEALQTNKYDLALSKYRDASIKVATSLAFLNSGQNLIFTTALTSMMYMACQGVVAGDLTVGDLVLINQLVFQLSVPLNFLGSVYRELKQSLLDMEALFKLQKQPIEIKSKPNAPPIKINDGEIKFNNVTFSYTEGRKILDNCSFTIPAGQKVAIVGPSGSGKSTILRLLFRFYDIDPKIKNSSVLIDGQDVRDVDLDSLRREVAVVPQDTPLFNSSLLENLRYAKPSASDDEINDIIYQVKLDTLIKSLPEGVHTIVGERGLMISGGEKQRVAIARAVLKNSAIIGLDEATSALDTHTEQQILNTLKDVLGSRTGKKTTSIWVAHRLRTIVDADKIIVLREGKVAEEGTHRELMAKNGLYASMWEMQEVWEDEQEEVEGSQQEKSENKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLH8</accession>
    <name>A0A1E3NLH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_68347</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ47002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018115.1">
        <property type="nucleotide sequence ID" value="XM_019163505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLH8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLH8"/>
    <dbReference type="GeneID" id="30180192"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="181" mass="20201" checksum="5AC1AB1B884944B5" modified="2017-01-18" version="1">MDFFTSRFSGSNAGPVHLQDDNATVANNNNASDDTDSILKLSYFERISLFIVCILGCYACYAICFIFFPVLSLKPKKFSLIWTIGSVLFLVAFAILNGPTNFFKHAISVERLPFTLSFVGSIVTTLLFSLVWKNNLLVIISCVIQAICSVYYTVSYFPYGRQGLRLTTGVARSQVENWLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLK5</accession>
    <name>A0A1E3NLK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_10055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018127.1">
        <property type="nucleotide sequence ID" value="XM_019159619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLK5"/>
    <dbReference type="GeneID" id="30176306"/>
    <dbReference type="OrthoDB" id="10417232at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="918"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="918"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="931"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="973" mass="114320" checksum="1BEEC10158ED6A45" modified="2017-01-18" version="1">MTLYWRNTTLNQLLGSVERPKDHPLDVYMAIEKLSVEEYAFGAKTHIPKFSVHNERSLQNFLSTINSKLGRRVYVSCDGLLNLGIVLSVETGYLVHISSTANIENNFIFGLASNVNSVLKNIVKSCDFKGEPPVDIALVKKWIKVLYLENKFELKINRYRPSFHIEYNTELALEKPILPLKHEVGIMNLYVEPNPLVPILNSYQCDFCLKSLAKIKHVMVHINENHQVPTGFKSNDFISFSHSQMTPKGHITVLVSEGFIKLEKIIISAHRKKVPAYFVPESVPKWKNQDLELSHNKLEYTINEISEFDEMFNFNCIVNFINNLKMNDINYYLDYCKVDERYRLIKNFLTKILFNIIVCQTRKYEFDTTLTSHRERTLKFYISSASRPSFVFGKISNPQRILNYAKAISEGLIILFGSLNLKRQKINLNCEIPDYKVHFSSSQKKLLKEIFKYVVANYKNKEVRMFDDEELKDTGLKLELEGDVLFFWNNLSKFFINISLQMEGRHTPLQTIFCLSTCNYDFCMKKLNFHRPPKRSRTFKALLYIIRLSFLSSINKKTDEYYNARITEATIKGGLRKIMEKLYYPTLYYFLKRKAAMLQPFFNAEKYTYVHPVSFKDKLVGCKILGLFSVNIMLNKSIEYIKAEIMYIRRFSEIQCDFDDDDSIRRVLEDYSEDTNNRRMWPLRKQKEHDPSYVENMLTKDFRRSISGITCLLIGYLVITNKPLSRFSEYLNIKTDALEFKNNLLSISTEEFNFTVDDESVVRYFKYYQVIRIFLLKVDEKIDDSFFFLKLDYVKPKTFFDLMGSRYTTYRYFCSFLKACHEKNNYRGERFSKNWIEIVRHGLRERSEGYLVINLKLIAPDHQKFVGSEDAPNVDPRSYLPIDEDFSLKDIIESGFLDYNFADEEDVEDDQDSDLTTANIAGTENKGNQDNFDDINDYDEEGDDDKSNKEDYETMINRIKMQLQSQTEKKSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLN0</accession>
    <name>A0A1E3NLN0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_10070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018144.1">
        <property type="nucleotide sequence ID" value="XM_019159623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLN0"/>
    <dbReference type="GeneID" id="30176310"/>
    <dbReference type="OrthoDB" id="3999982at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="123" mass="14168" checksum="9B31DCA1220A6F6B" modified="2017-01-18" version="1">MSAKAAFPSIKHFWKQGLFLTVVAVTSAYFVKSNVTIRRRKQYIKEASSYDSLMQERVRERSGDSISTVKPGFPLDDGIERYQRKSEYEGAGLSYRSRRTGDKFTISHFFTDYSNDSNSKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLR1</accession>
    <name>A0A1E3NLR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15069</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018183.1">
        <property type="nucleotide sequence ID" value="XM_019160649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLR1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLR1"/>
    <dbReference type="GeneID" id="30177336"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="583" mass="64047" checksum="27958A4055047D3B" modified="2017-01-18" version="1">MTGKFRRESQVNESFSRAAIPILSEQHDASQHYGSIISDQDSLIQRRRASSMYSTRARQLHLASISVKNGRYQPIDTDTTTEVKMMMQSSIPLIITLLLQYSLTVASVFFVGNLGSNQLAAVSLSNLLANISSFGLIEGIASSLSTLCPQAYGRKDYKMVGMHSIRCFLMLMLLYIPIFCFWTWGAYPLLSAVVTEVEACILAAQYLKVLVWSVPGFIVFEVLKQYLQAQGIFHASTVVLLICAPFNFLLTYLLVWNKHFGIGFLGAPTAVAVTNTLMALMLLFYTCFINGYQCWCGLSMEVFKNWSRALKLAAPGVLMIEAEWLAFEIISFASSRFGTESLAAQSIVSTVCVTIYQIPFAVSVAGSTRIAWFIGSASKKAALISTHAAIYVSIGFGIFNCIVLGTGSSSIAKIFSQDEAVVNLASKVLIIGAIYQIPDCLCCVLGGVLRGQGRQYIGGWLNLFSYYVLALPVAFLFGFHFELELFGLWIGMIVALVFVSTCELFFVLASDWETIIKDSLVEDLDGQMPHTSHNDSGYEDAINDDTLSLRPSRSYGSLIDHNLLSPTISSATLGQLDPSVLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM41</accession>
    <name>A0A1E3NM41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Chitin synthase</fullName>
            <ecNumber evidence="2 7">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_33013</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the chitin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 7"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017761.1">
        <property type="nucleotide sequence ID" value="XM_019162794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM41"/>
    <dbReference type="STRING" id="763406.A0A1E3NM41"/>
    <dbReference type="GeneID" id="30179481"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902404">
        <property type="term" value="P:mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF38">
        <property type="entry name" value="CHITIN SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="646"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="684"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="715"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="753"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="789"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="900"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="931"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="9">
        <location>
            <begin position="236"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="983" mass="111126" checksum="19AA80ADDE7D7C7B" modified="2017-01-18" version="1">MSDPFRQNPFIVLQSPVKKPEEIYTPSGSPKRVSPSKAAMKYSPAVSFRRDSNNFMNSAVGRSFIQRADESPLRSPPTARKLFTEQESPKRKAALTTIIDPFNAPEAESDNENDIISHYSIFNKTEPGELKRSINSNRTELETPKPVFSNSLFAAAVQSSTNSELYKNKSAVSSYFSSDQSTIDVPMDQDFQYSNGGERFSNSNGSDFFDKRLMDKAPMVQPRRNNYHNKQTVFRSRTGNLVLDTPVPGLLHDLLPQKDSDEFDYMKYSAVTSDPDDFAEDGFTLRSIELERETEIVICITMYNEDEVALTRTLYGVFQNIAYLTKRKRSTTWGEDSWKKIVVCIVADGRNKISPGVLEVLGTMGVYQEGIAKSFVNQKEVKAHLFEYTAQVSVNEDLKIVGDKDSTPVQIVFCLKEKNAKKINSHRWLFNAICPVLNPNVCVLLDVGTRPNVGAVYNLWKAFDLDSNVAGAAGEIVAMKGKYWSKLINPLVASQNFEYKMSNILDKPCESAFGYISVLPGALSAYRYSALKNHPDGTGPLKAYFQGETMDNADMTNIFSANMYLAEDRILAWELVAKKGAQWVLKYCKDARGETDVPETLSEFVLQRRRWLNGAFFAALYSLRNSAQIFKTDHSVMRKVFFQIEFLYQGLTLLFSFFAIANFYITFYYLAGSIITIAGIGGKVFFEIMNYICICTLLGMLVISMGNKPTGAPMLFWVCVILLTACGLYAIISGFYFLALMIHQHQAGNAGGLSFASICVSLASTYGLYVLMSVLYLDPWHMISSSVQYFMMLPAYTCLLQIYAFCNTHDVSWGTKGDTEVAKSLGSAIIETDEKGQQYIKTGVLEQQDIDSMYSELVLKLKERRKKPVNMRNKKDSKDGGKKKLQISKEDYYRDIRSRVVLFWVIANVILVMTITQIWKADSIRSNKYLAFILWSVFGFSCFRFLGSFTYLAHLAVRRIIVTKHKWELKKNEKVPKMNQVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NME6</accession>
    <name>A0A1E3NME6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial cytochrome c oxidase assembly factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_30963</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018408.1">
        <property type="nucleotide sequence ID" value="XM_019162664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NME6"/>
    <dbReference type="STRING" id="763406.A0A1E3NME6"/>
    <dbReference type="GeneID" id="30179351"/>
    <dbReference type="OrthoDB" id="18175at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13184" checksum="723DB7A444B016C8" modified="2017-01-18" version="1">MPKLTRSQFELAKFTFYLMTPITIMYYVGIDTDRKFNVPGYWPDPDTLNKIPKEPHEIQAELARIRQAKIEKRRRLEEKAKLLGITPDEEEEQDRAAADGSTQDAVEAVLTTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMS2</accession>
    <name>A0A1E3NMS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein PXR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PinX1-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein pxr1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15383</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018548.1">
        <property type="nucleotide sequence ID" value="XM_019160801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMS2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMS2"/>
    <dbReference type="GeneID" id="30177488"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032211">
        <property type="term" value="P:negative regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF31">
        <property type="entry name" value="PROTEIN PXR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="G-patch" evidence="10">
        <location>
            <begin position="25"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="151"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="191"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="297" mass="34678" checksum="1B3BFE895585000F" modified="2017-01-18" version="1">MGLAGTRTKQRFGLDPRNTHWSNNTHRFGHQHLVKLGWEPGKGLGLTNDALTSHIKITMKTDNVGLGATLAKKRAKQDEFDSGENMHLDAFQRLLGRLNGNEEKISKEVDRKRNERILNGRWGINFVLGDTLSSTWDKENRHLISTEVVSNKKRKNEETEPVAEPPVKKSKKEKKEKKEKKEKKEKKEKKEKKEKTEKKEKKEKTEKKEKKEKTEKTEKKEKKEKKEKKEKKDKKEKKHKNDKTSKDVTRDSMLKPKTEVSSTNVLGSKMALRSKWIRQKRAAVMDEKALQEIFMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN33</accession>
    <name>A0A1E3NN33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">DNA repair protein RAD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_20538</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018171.1">
        <property type="nucleotide sequence ID" value="XM_019162160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN33"/>
    <dbReference type="STRING" id="763406.A0A1E3NN33"/>
    <dbReference type="GeneID" id="30178847"/>
    <dbReference type="OrthoDB" id="31113at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048256">
        <property type="term" value="F:flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09904">
        <property type="entry name" value="H3TH_XPG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000030">
        <property type="entry name" value="Flap endonuclease GEN-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000061">
        <property type="entry name" value="Single-stranded DNA endonuclease (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="15">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="14">
        <location>
            <begin position="754"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="655"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="986"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="989"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1012" mass="115738" checksum="D816E490EA19336B" modified="2017-01-18" version="1" fragment="single">MGVHGLWDIVGPSARPVRVEALRNKKLAVDASIWIYQFLKAVRDKEGNQMKNSHIVGFLRRICKLLYFGIKPVFVFDGGVPTLKKETIRKRKEKREGNKESIEQTAKRILAKQLQLFAEGKLKRPTTSAPNVTRREIPTEELYYDAYYDDQVGDSDVGPPKEAEVPQEKESRLFRGQDDYDLPNRGPIKVAEDDKRVMTGYEYDRLTQDINDDLDGLDLDNIDPQSKEFENLPLSTQYIALSHLRLRSRLRMGYTKDQLQSLFSDPMEFSKFQIKMVQKRNFFTQKVMNVTGMDGDSKQDVVSRRIASDKNRAYDLRRTGDGYALSISKDVVEGGSSSRPIFLGDVKEFVGVEQESPIELDASEEDVDWEDVEVVNKSGKLEKSKADSDIEVKDPLFMKDSDQSSDENDDIMKKIKYLYEYSNTHNKGKGPKNTAIEDDELKQAIEKSKQDYLNTLNEEKIGSGVRGTLDDEAPIILSTSALAKTLKLPKLNMKDNLLFGRPREKPSKVADLENKSKKNDEINPINEKRKALPLPEWFSNQTSLGPKPTMQVRKPSRNLTEDEKAGLVSYGDIENYISSEDENTVIDLNESSHDEKLIEVDEEGSDKNQHFVIDITESPKHLKDNSYLKTPNSNIIEIEDKKADDGIVISQSLVSKDFENETEPENQADKPQLDDDSDFSDEEEVNLLLDMEEENLAYDKFFSNIGGIKVDSNKDEGLWTFTDEAKLQERLRKQKRDADEVSTTMIYDVQDLLARFGIPFITAPMEAEAQCAELELIGLVDGIITDDSDCFLFGGDRVYKNLFNEKNFVECYQDADILGELGLNRSSMIELAILLGSDYTDGLKGIGKVTAVEILAEFGNLENFKQWWVDYQNGKIDETKDTPLRKKLRKTLSKSLFLGNDFPSRLIYDAYLHPEVDHDKTEFKWGYPDIDKLRTFLAFYVGWNKEKVDEIILPVIKSLNKPQLTIEEFFPVEVIQRRRQLNMSKRIKSATDKLKRGRSKQTDDENQGKRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN94</accession>
    <name>A0A1E3NN94_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15518</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018695.1">
        <property type="nucleotide sequence ID" value="XM_019160860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN94"/>
    <dbReference type="STRING" id="763406.A0A1E3NN94"/>
    <dbReference type="GeneID" id="30177547"/>
    <dbReference type="OrthoDB" id="1724687at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000554">
        <property type="entry name" value="Ribosomal_eS7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01251">
        <property type="entry name" value="Ribosomal_S7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="188" mass="21186" checksum="F1B62B4427B1FB6D" modified="2017-01-18" version="1">MSAAAKVLSANPTELELQVAQAFIDLEANSDLKAELRPLQFKSVKEIEVSGNKKAIVILVPVPLLSAYHKVQIRLTRELEKKFPDQHVVFLAERRILPKPARGSRLGQKRPKSRTLTAVHEKILDDLVFPTEIVGKRVRYMVGGNKIAKVLLDSKDSNNIDYKLETFQSIYQKLTGKQVVFEIPSKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNL2</accession>
    <name>A0A1E3NNL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71719</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018788.1">
        <property type="nucleotide sequence ID" value="XM_019163991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNL2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNL2"/>
    <dbReference type="GeneID" id="30180678"/>
    <dbReference type="OrthoDB" id="10266128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000006">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CP-type G" evidence="10">
        <location>
            <begin position="173"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="526" mass="58360" checksum="EDA0C24DF02DF8FF" modified="2017-01-18" version="1">MRVKKPTSKRMTTRLREGIKKKASAQRRKEKKTAKKDVTWKSRNKKDPGIPASFPYKEKLIEEIEQRKQEREEHKRQMKLQKRNAQINASAEDIVAEEEMVVEEDDGAGGMAALLESAEQAARDYEGEDTAGAAENDQDRMIEDGEELEVIDHEIVYDDDEEASNNNEIEKSRKQFDKVFKSVVDIADVVLYVLDARDPESTRSKKIEEAILQSQGKRLILLLNKVDLVPDEVVKQWLDFLGSSFPTIPIKGSSGAVTGKTFNKKLTQNATAGNLLLALKAYAQKSNLHRSIVVGVIGYPNVGKSSIINALTSQRGKNNTACPVGNQAGITRTLREIKIDNKLKIIDSPGIVFPENTKRGNSAKSKKDYEAKLAVLSAIPEKQIEDPIYAVGFLLKKLSQDTTMAENFKQFYKIPAVASINLDDFTRKVLIHIARNQGRLGRGGVANLHAAACIVLKDWRDGKFQGWTLPKSSKGQAASAEDDEAHGVNGGRGVTPPAKVEQTTVVKEWAQEFDLDSLFSDVFGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNL6</accession>
    <name>A0A1E3NNL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Amino-acid acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="5 13">2.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glutamate N-acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">N-acetylglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_10144</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">N-acetylglutamate synthase involved in arginine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.1" evidence="5 13"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018226.1">
        <property type="nucleotide sequence ID" value="XM_019159636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNL6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNL6"/>
    <dbReference type="GeneID" id="30176323"/>
    <dbReference type="OrthoDB" id="5585968at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011190">
        <property type="entry name" value="GlcNAc_Synth_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF4">
        <property type="entry name" value="AMINO-ACID ACETYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007892">
        <property type="entry name" value="NAGS_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="466"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="348"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR007892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="626" mass="70836" checksum="3186A1245D378078" modified="2017-01-18" version="1">MQLTRFSGRKIGVYRKSIFGGRRNHSASLLRAEDFDDESNLLKHSIASNEKHQLILTILNSTATKREARSYLSKYRLLGENDIYKNRQRIVNQDQSDSNSPLVTNKQYSKYIGKLLEKHVDPLYNYNNNIEQNPNVEAYNRRLLNSESPVLMTTPTSPKNRINDASNEIKLTQTIRACIFRIRKLHKWDTDTVKNLAVVLRKAMGLGASPVIIIDGDVIEGYKGEQVKKEIENYVDSKLESFVEGLGNFLPNRIIKGCFDLAENGDLQTIVPEMIGVPIFSGIIPIVSPFAVREGVLQVVKGFPTTRATVDCLESSEMKEILTIEKVIFVDEVGGFPSVERSEGSHVLINLLQEFDDIKNELQKELELSEEERETHLSNLMEMRQLLDLSPDITGILTTPEMATREEEKQVGKETNPIIYNILTDRPTISSSLPVSLRRTPQLNTTVVKKGISISVYQSSDPSKGLDLRAMEREGKVDLAQLKYLIDNSFGRDLNMDHYLNRINGKIAGLIIAGDYEGGAIITWENLGERSVAYLDKFAVIKKLQGIPGIADVVFKAMLKSFENELLWRSRKNNPVNKWYFERSKANYSIDGTQWRLFFTGKQELSVSLLAGYIKVCSSIEPSFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNN9</accession>
    <name>A0A1E3NNN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_27628</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018812.1">
        <property type="nucleotide sequence ID" value="XM_019162505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNN9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNN9"/>
    <dbReference type="GeneID" id="30179192"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071468">
        <property type="term" value="P:cellular response to acidic pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071248">
        <property type="term" value="P:cellular response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000181">
        <property type="entry name" value="C2H2 type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002218">
        <property type="entry name" value="Transcriptional regulator CRZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="81" mass="9963" checksum="F5CB226700CB3EDA" modified="2017-01-18" version="1" fragment="single">EERKYVCDICGKDFTRPYNLKSHLRTHTNERPYACRKCGKRFARQHDRKRHEDLHSGEKKFQCREWGCNKRFARTDALRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNP8</accession>
    <name>A0A1E3NNP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ureidoglycolate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_26364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-ureidoglycolate = urea + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:11304"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57296"/>
            <dbReference type="EC" id="4.3.2.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018411.1">
        <property type="nucleotide sequence ID" value="XM_019162444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNP8"/>
    <dbReference type="STRING" id="763406.A0A1E3NNP8"/>
    <dbReference type="GeneID" id="30179131"/>
    <dbReference type="OrthoDB" id="10266039at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004848">
        <property type="term" value="F:ureidoglycolate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050385">
        <property type="term" value="F:ureidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20298">
        <property type="entry name" value="cupin_UAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.480">
        <property type="entry name" value="Ureidoglycolate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047233">
        <property type="entry name" value="UAH_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007247">
        <property type="entry name" value="Ureidogly_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024060">
        <property type="entry name" value="Ureidoglycolate_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221">
        <property type="entry name" value="UREIDOGLYCOLATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221:SF1">
        <property type="entry name" value="UREIDOGLYCOLATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04115">
        <property type="entry name" value="Ureidogly_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19044" checksum="BC16B0C1AFDD5490" modified="2017-01-18" version="1" fragment="single">FERYGVAMSVDHLRDSVPIDAANQGSAIKLIKVAPCVNNFAEAPSKHVATTNWNIFRSCVIPEKWTTDSDGLIYNLTVLEKHPYSTQTFVPMGRDPEEDAYVVNVAPDKDGQPDYENVEAYVFKGNTAVTYNLGTWHSPMVVLNKTTDFCVVTNENDVSEENCVEVFYSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNQ5</accession>
    <name>A0A1E3NNQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pop1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71746</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018820.1">
        <property type="nucleotide sequence ID" value="XM_019163996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNQ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNQ5"/>
    <dbReference type="GeneID" id="30180683"/>
    <dbReference type="OrthoDB" id="442863at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039182">
        <property type="entry name" value="Pop1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009723">
        <property type="entry name" value="Pop1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012590">
        <property type="entry name" value="POPLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731:SF3">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06978">
        <property type="entry name" value="POP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08170">
        <property type="entry name" value="POPLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pop1 N-terminal" evidence="5">
        <location>
            <begin position="86"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="POPLD" evidence="6">
        <location>
            <begin position="597"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="919" mass="103898" checksum="EC1BE9ED0439F63F" modified="2017-01-18" version="1">MQSLPKKPSTGGSVAGGGKNNTTSNTASNNNPTSKRTKLYNSRQIRTQIQDESFTNPPKGGTTAQQLRKMLSNGSMIPNKLSIPDYLSAREFEIRALDSAMIKSKNSGASRVFQTLPRTLRRRTASHNVRRIPKRMRRKALREMGLQSKAGSATTTLGTKGVTVMGKPIKPKIGRGRKRWALIRKMKLLKYAAKWKIKGRLPNSQWISVSQINLRKKLKLLKKDLKLLDEKHDPEEAENSNSHKWVKHDVTKIKKLAFNIHNQLGSYDNTSVNKLSKIGKLTSVQYATRQKNFKWLPTHIWHAKRAKMMKRWEWTIPLEPTQRCYRSTSRASRLKGAVVSDTSYIGSLVINAYSKGSMEKVLKYISVITKNKVKIYESCLSKGIAWEGYVYDLHDSGDPIGRMTLVFVENSLQNTFRVMARLHPSIFSTVFSQLVGNLEGVEEVTIHDCKYSIGSIDLTGPKSLTALQSILQCRQENDSQFESFLKLHKLNDLTSVPENTIFTFNVCDPRFKTKPVIPAKSKINYDDQLDILIALKSKKKKSLSDDILNPESRAQTYDNQMSLKELGKRRNLHPGEAIPIKENDASICVMLIKSYEQWTILLPWFWVVPFWYSLVHVPHVQFGGFKQSEQLRLERKLLGWSDMVFTTNGYVQCELEREQAERKWEKKPKSKRVTYAKLKVDGLGSGETLSPFGLDWRGLQVIRLVTKKLKLEGNLGGMKNKQNNRIVTDDKLNIVPQTLSDLPIAVKAIQAAEQQLKKQNLHSVLLQYKPITLATNITTGIDHRKLTFNIKQLPALAVTPIYLTCVGKGNVVSNARIYEVNKKYENFWKETASGVVTNIRGNSVRVGSVAEAEKLYPKLHNVVGLVSSATFSLVEGKSISIGFVDSDAIDGDVSGYFLVRNVASTSYILMHWNCINMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNQ7</accession>
    <name>A0A1E3NNQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_31955</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018835.1">
        <property type="nucleotide sequence ID" value="XM_019162733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNQ7"/>
    <dbReference type="GeneID" id="30179420"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140824">
        <property type="term" value="F:thioredoxin-dependent peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000010">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029759">
        <property type="entry name" value="GPX_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00460">
        <property type="entry name" value="GLUTATHIONE_PEROXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="161" mass="18024" checksum="AF211DCE7C6863A3" modified="2017-01-18" version="1">MSKFYSFTATANNGTPFPLADLKGKVVLVVNTASKCGFTKQYADLEELYKKYHEQGLEVVGFPCNQFAGQEPGSSEEIAQFCKLNYGVSFPIMQKIDVNGDKEDPLYAWLKAEKPGLLGFKGIKWNFEKFLVDKEGNVYQRYSSIKNPASLSGDVELLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNS4</accession>
    <name>A0A1E3NNS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018845.1">
        <property type="nucleotide sequence ID" value="XM_019164000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNS4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNS4"/>
    <dbReference type="GeneID" id="30180687"/>
    <dbReference type="OrthoDB" id="5596576at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="228" mass="25426" checksum="B6CA24EAF46AB28D" modified="2017-01-18" version="1">MVFEDIESTLEVGTGSSSRFGGLTERWQRLRKEIVRYRLHVYLPIAIISIVLFSLITIFIVRVEPNLGVGVAEGTQFNADNVRLLGLGEQGGIDLQVSGINSNNFTNIDDFWIRQYFKKGGFVIRGLNLKIDELDLIVYDASKGEDLKLGKIEIQPFYVKIVDGANTALDLFVTLWPNAKGTRGLLRKILLDSGAKLRLRGDANVKVYVLNGFLPVSSISIPLDVEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNV7</accession>
    <name>A0A1E3NNV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_30783</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018875.1">
        <property type="nucleotide sequence ID" value="XM_019162656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNV7"/>
    <dbReference type="GeneID" id="30179343"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010607">
        <property type="term" value="P:negative regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF153">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="11"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="266"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="37202" checksum="DC9A8A8FCB4F1C0C" modified="2017-01-18" version="1" fragment="single">TTKGKYSLNDFQLIQTLGTGSFGRVHLVCSVHNGRYYAMKVFRKSHVVKAKQIEHTNDERRILAMCRHPFITRMWGTFQDCKSIFIIMDYIEGGELFSLLRKSKIFPNQVAKFYSAEVLLALEYLHSKNIIYRDLKPENILISRSGHIKLADFGFAKQVETTTYTLCGTPDYIAPEIIAVQPYTKAVDWWSFGILIYEMIAGTTPFYDPSPMKTYENITKCQVVYPNHFQMDVIDLLHGLITKDITFRLGNLKNGVDDIKNHPWFNEVIWENLLQGNIETPYEPNIPAGVGDASQFEKYPEDRYDYGISNVPDEYGHFFPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNZ7</accession>
    <name>A0A1E3NNZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15233</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018366.1">
        <property type="nucleotide sequence ID" value="XM_019160731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNZ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNZ7"/>
    <dbReference type="GeneID" id="30177418"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="6">
        <location>
            <begin position="71"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="587" mass="67682" checksum="3D157B0A85412FFB" modified="2017-01-18" version="1">MSDEALSLSNSDEEIYLRNHPLNDRMIELAKYIIGNYDFMKRCTFPIYQYALLLNFYQKIDGEKLYTQGETHLSSSILVQMAASLGMNRDPSKFANFSCNAICGSLGRKIWHTLVSIDNFQYFQEGVPKSIAESSCDTELPQFDPSFSNNNDPNIEKLTIQLIHTRHEIEAEIKPIADKICDIHNPPTVLSVLESLENFEETLVSRFDYLNTLFKKSNNNTYYNRLEKVGNTLIVFEAISFIFTILLHLGLKFESMKHFPAAKFFSSKILASSMSFLSVIPEIASSSHEFFGVGFDFVVIPSTEIIMCKNMVFITSNYVKYSIFKQKLLKYDPLDAVKLKLINQILHTVLYDVFYNRHLKVLKLLASNFFFAWRILKAQSYIFTLLKDGLLSYENQRGAFNFVETYTADDLMETLQLLDLSNYSNPNKPDCYFTSILRKYKADICDEDPRNEELFSNLNLELRRKKDELSNDSTMGRPNICRSLFNNDGKSSDNLFGSIFSVEDDQFWMDILQSNKLNDPQNGIFRILDNLYPSQKGNYQPELIFSDVEETPPLPEVIADPTVVGINDEKDELKNQFVDEAIFDFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP28</accession>
    <name>A0A1E3NP28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nucleotide-diphospho-sugar transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15714</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018940.1">
        <property type="nucleotide sequence ID" value="XM_019160933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP28"/>
    <dbReference type="STRING" id="763406.A0A1E3NP28"/>
    <dbReference type="GeneID" id="30177620"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="70"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="663"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="82734" checksum="3884D783884EE433" modified="2017-01-18" version="1">MMFIRRSSRTKLLVAAIATVAAIAMFTNRHHSAAYIDDLRKTALFPYGADTPTETEGVAGVAESTRTAHPADGEVVYPPPSHGTEQLAKPRESSEVAEKQEHGHGKAGPAGGEDAPPETLFDFGEAEIRAIHNVSTERFLSGDPHAQGFFGKLFDLVLRNEMSHPLKRRQGLDGNGKPHIDNVHFKSDPDEILSEEQLLRFFDFPPEFVDDLTVKHANVLNSLPDYVPSFYAGDGYAIVGGGHYSWYSLLAVKCLRIAGAVLPVEVILPTHDDYEPLLCETMLPLMNARCVDAENIFGKHRLDQMKISGYQLKSLALLASSFRNVFLMDSDSYVVTNPEPLFKSELFKMKHMLAWPDFWRRTTSPVYYQIAGINVTDEPVRHLNDVFTDINKMHLFKTEDPNFNVKKDVSFHDRGNTLIDWSSESGQLLVNKDIHFKTLLLAFYYNRDGPFGYHPLLSQGGAGEGDKETFVAAASRLNLPYYQVYKKSDGAYGFWNLLNSFEHGAIIQYDPVTDSENVVKAVNRIKHDQKEQGDQFVYDYSKYFIEGIRAEESKPLFYHCHDPKFDPYLIRERNIMFVREHGKILEKRRRVLGEDFPRGDVDLELNLWEIADDYLCRQKLHFSIFDGKDKDILCKEYIPEQLDFLRKSHDYIVEHYNPDTSRANLDGSNDVFGEKKKSEGETAATRLESDALQKAEEEKQVMENEAAEALEQVKAASAAAEEKAEQAAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP48</accession>
    <name>A0A1E3NP48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_120146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018965.1">
        <property type="nucleotide sequence ID" value="XM_019159869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP48"/>
    <dbReference type="STRING" id="763406.A0A1E3NP48"/>
    <dbReference type="GeneID" id="30176556"/>
    <dbReference type="OrthoDB" id="2420608at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018465">
        <property type="entry name" value="Scm3/HJURP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10384">
        <property type="entry name" value="Scm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="235" mass="26173" checksum="54E0F7B214BCAC28" modified="2017-01-18" version="1">MEYTDLDRRRQTSLHKVRDAWKCIIKKYSALSENEQGDVVDIATGEIIKDTGHLRSLRQDDDNLWAETEPPPRAHGAGDGHGDEGGDPGRKRAKSSSKTAIEIVDLGSASPPPSQRRRSQPPHSQPGDTSYVVSSSQHMTRSKVVGDDNLIVRRPKTHSGRHPSDRHHHPRSKRSGALDSTANDPLNMLSSSVDLGTPSKVRRLRRALDHSFQVKRSSGRRHLHKLILHEKLGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP49</accession>
    <name>A0A1E3NP49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_71885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018978.1">
        <property type="nucleotide sequence ID" value="XM_019164038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP49"/>
    <dbReference type="STRING" id="763406.A0A1E3NP49"/>
    <dbReference type="GeneID" id="30180725"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="210"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="36023" checksum="17E122A0069FE424" modified="2017-01-18" version="1">MKTDLPKESQDIIVQDVPEDIDPFPNDAGLNNTTQITGTDLHLRAPIETANIKENCAQDQIDHEIGKLQTNSTQISEDRLDCSTMSQVNVKDFAYALSDPKHFGIYPSDDEDEDVDEAPDGDDYSHDGINEKDLNYNENSDYHTQDGINHLQNSIVPHKFANSYSKIPDSSIDTDLQTNEVRNTYGKPTIENGTKVGRQESAYPTEDENNDILHAVALYEFTPENSNELPLSPDQLLIINYECGDGWLVAHDPFTGQTGLVPSEYIRILEIETTEEDCDAEVYSEFAEDAKDAQQFMPEILADDGSYPPEQGITENINKLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP66</accession>
    <name>A0A1E3NP66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15746</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proline oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018980.1">
        <property type="nucleotide sequence ID" value="XM_019160946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP66"/>
    <dbReference type="STRING" id="763406.A0A1E3NP66"/>
    <dbReference type="GeneID" id="30177633"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="6">
        <location>
            <begin position="134"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="501" mass="55077" checksum="F38E9738251DA805" modified="2017-01-18" version="1">MYRRILLQQSVVRNGLSKFVPLAQKQGASGAAAAAAATMLQHRGISNTTEKKISSAGQPTIPPASLDGVKSAEKTYLDTLTSGELFGYLVIGMATINRPILDLVIRLFPYTPLWIIRALVYQNYCGGDNLPQVLKTGERLAERGIRNMMVSYTVEACDGKQMSVSIEKIIEETQRSVSEALSPHTAAMIEEVVSQAEPSQISNAINEVPPGYVALKPTGLIDGAAEVLLNYENPKYADRFNELVKNCESVCDIAVAENQKLKKKYPAREAPFVVIVVDAERNDLQQGVYKLQRDLMAKYNRSSVPGARPTLGDACVVGTVQMYLKESSSILKLDEELAKKGNYILGWKLVRGAYIHSEPNRGVIHDTKKDTDVNYDAGISQTISNMNSENPTVGHLVVASHNGSTQLKATTLLEQLNGEQAQKVRSNVVLGQLLGMADNITYDLIKNHNTKNIIKYVPWGPPLETKEYLLRRLEENGDAVRADSGWPLVKGILGTFMKRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPB1</accession>
    <name>A0A1E3NPB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyridoxamine kinase/Phosphomethylpyrimidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71457</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018496.1">
        <property type="nucleotide sequence ID" value="XM_019163930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPB1"/>
    <dbReference type="GeneID" id="30180617"/>
    <dbReference type="OrthoDB" id="10028886at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19367">
        <property type="entry name" value="TenA_C_ScTHI20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="303"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="57991" checksum="DFDF03A35C30FDEE" modified="2017-01-18" version="1">MTIAGSDSSGGAGIEADIKTITTHKCYALTNIVILTAQNTKGVCDVTNIPGTMTSSILEENFSDIYIDSIKTGILTEESIPEIKNALDKYHFHGNLVVDPVMVSTSGYDFVNNKILEILTRYLAPYTTVITPNLIEAKAIINTLSGKVQYNGNCLEALEDVFSICNQIHKLTKIKNVLVKGGHQRWKNDAKLLTDVLYISEKNTYYVFYSEMLDSSSTHGTGCTLASAIASNLAHGLSLINAVGNSITYVQNGIKTAPNIGSGHGPLNHLQSVRQYNYEVLVNDEKYALPFKEGQAVQYMCNHPEISPFWEKYTHHPFMKKVGDYSLPLENFTKFVEQNVIYLSNYSHIIMYMAAKGSWKEDLCAVVDKLTTMAQEISKYTTILKKLGYSDKLIEKLKATKECQVYVDALLDVAKDAGDVFDIAVSLTPCFYGYLAACANVKHYPKVGDICSDDTKIELYEDWLKQNQSEWYSSAWIKGEADLNTKFRVNCLSDAKLTRAVQLFKKFTIMEINFLDSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPE2</accession>
    <name>A0A1E3NPE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LMBR1-like membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71976</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019087.1">
        <property type="nucleotide sequence ID" value="XM_019164062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPE2"/>
    <dbReference type="GeneID" id="30180749"/>
    <dbReference type="OrthoDB" id="203099at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="664"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="712" mass="80957" checksum="9028387C36D83875" modified="2017-01-18" version="1">MHVLKTLSAAPVLPLLAGCSTIALLVSFFILYRLTNVRTISVPLNITLTISLFLPLSTTYLLPIDMATPSSSATISILWKTNYWITYVSTWVILPFWQYFLSSGQFTLLNRIKNSAYSVLKFLFLLIIVGLLFLFYVLLYHRDALDFKYLKSLLITTSHIYSLTMAIWLMAHGLNHLPKAFWMTSYSKKLHLDYIKLPELQIHLEDSKFELRDVCNKINSLYEILDLNPSLNSELDVSLRDHILYLHAKIPEEFKLDRNLRSSNNNYQFLMDSIGVSTNEIDDVFLSKLNEDLKWKVWDYKHSKSIFEHKVLDIVYLEDVINYMNNSSFNTSFDNANTSLEIEWRSYKICWPRWVFVYIWPLLYKLIAVGFAIIGLVIIESEMMHGTRLSVMSWIIQNTSTLSCFKMVVILIIMMSCALLSLGMVKLFNIYKVEFNSNSDPVSSVFFISYALRLTIPLGYNFLMLLDTSVTSNSAFLKFVSGNLELIRIGDLLNDIIPRLILVPVLMSFFGVWGKLRRWLDGYFLFDYILEEMDFEDDTTERPTDLRANNDIESVVGSTDKTAALLQEGRTISQRYVSNGNLQLNDSLPRISPPSSSMSTSQFGRNAVGIFTFPFRLVKAAVESAGGLFGKLFGRSRDNQGLDSSIDGLRAYNIPSAGGFVLESRRNSQASEQSRLSGNSGLSGVSGLSGESGTEYDADNRVLGDEYISNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQD0</accession>
    <name>A0A1E3NQD0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_125555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019388.1">
        <property type="nucleotide sequence ID" value="XM_019159945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQD0"/>
    <dbReference type="GeneID" id="30176632"/>
    <dbReference type="OrthoDB" id="3987408at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018854">
        <property type="entry name" value="Psome_chaperone_3/4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10448">
        <property type="entry name" value="POC3_POC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="130" mass="14512" checksum="78CC0B59841633D1" modified="2017-01-18" version="1">MPATKDTQTLTAKISPVFGDEFEVAATLPHKRPSLRERNRIPISLHLSKVGNNAPLGCYVYTIVDSRTGKVYQTLLNNSEEQLVDMTRKIGGLVSKKFSVPTYISMSGDWSLEDMVTTVKEVVKFVDESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQG6</accession>
    <name>A0A1E3NQG6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019399.1">
        <property type="nucleotide sequence ID" value="XM_019164135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQG6"/>
    <dbReference type="GeneID" id="30180822"/>
    <dbReference type="OrthoDB" id="10648610at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="274" mass="31550" checksum="01FFC23D3F3C21FB" modified="2017-01-18" version="1">MPIENNAYVAIAIIPIALVLVDDFKFHSISIPKSLSFTVIFIVASLVCQNIYTNQMTQLQRKTMNHIKKRRTEPTKNKKKRKSLIHLVDNRKSFDLTKWKSKNHPFANRYSSYSEKNSPLSKPYKRVNSWHGELLNSDNPLFPRERIALMIPSPPSSSQGETFENNLYSGDFRSSVLSTSSTIFENDNSRITIDSDSNIVDMSIDLNINCDGQDSSFFQPASQSKTRYIATYLYDRFRKSKAENSSYNKNCALDPSHLKPFNLTNRDIHDRSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQX4</accession>
    <name>A0A1E3NQX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor eIF2B subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">eIF2B GDP-GTP exchange factor subunit epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019578.1">
        <property type="nucleotide sequence ID" value="XM_019160194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQX4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQX4"/>
    <dbReference type="GeneID" id="30176881"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04197">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05787">
        <property type="entry name" value="LbH_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11558">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000066">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035543">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051956">
        <property type="entry name" value="eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044123">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="9">
        <location>
            <begin position="535"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="439"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="712" mass="80418" checksum="2095EA6B23F35607" modified="2017-01-18" version="1">MPPKQNKETEDEPKIQAIVLADSFQTRFMPLTSVKPRCLLPLANVPLIEYTLEFLAQSSIVDEVYIMCCSHADQIQEYIDESKWVNLSSPFKKIQTIPSKESRSVGDAMRDIDSRGLITGDFILISGDVVTNVDITKVLEDHKKYKQSDRDYICTMVLTQAAPMHRSRSNIEPACFIMDKATNRCLYYQDLPPINGKKTSVNIDPEVLEDIGEFEVNNSLIDCHVDICTATVPTIFQENFDYQTLRSDFVKGVLGSDLLKKHIYSYITTDQYSARVESWQTYDGICQDVLERWCYPIVPERNLLEDQTYTYGSQHIYKENDVILSQSCKLQSCVVIGKDSFIGDHTEIQNSVVGRSCKIGNNVVVENSYIWDGAIIEDGCIIRHAIVANDAIIRKNVILNPGSVIGFGVVIDENVIVANNVRIIKNPIRSATSSTLISSEEEDEDEDKVEDDEFDVPSRVVSEMALRDPNVVGYNGLGFLYEDDEDSQGGNGGIVYEMAELNLSDSSIASSAITVHHTNKKKKRTHSSASGFVSEDEGESFDIEAIATVERAMENNHDLDTAMLELNTLRMSMNVTYHEVRESSCLAMVKRVNHFVDTDTLGVKNAAEKIFRQWGKMFERQVFDDEDQVDLLLILQNICHKLDAEYKALLFGCIAVVLYDIDILDDENIIAWWNSEESKNVPEIREKIAKWIEMLMADSSDEGSEEEDSEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRA8</accession>
    <name>A0A1E3NRA8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_31202</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019331.1">
        <property type="nucleotide sequence ID" value="XM_019162681.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRA8"/>
    <dbReference type="GeneID" id="30179368"/>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022125">
        <property type="entry name" value="U3snoRNP10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12397">
        <property type="entry name" value="U3snoRNP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 9" id="KW-0698">rRNA processing</keyword>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="564"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="BP28 C-terminal" evidence="11">
        <location>
            <begin position="1494"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="1736"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="830"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1776" mass="199495" checksum="EF4D0A5D6718D809" modified="2017-01-18" version="1">MTSLAEQLAGIAQESKAVALDRKRRTKIHSVSFLHDPKFAATQDYESIYYDALDALQRLESLDRRFARFENSIFSETSIQIDRQVQSKEENDDLNKTIDAFLSLLSPYWNLAIAVRAVEWPLRRFQLNIFNSEHLLLSTLAYFDQPIFPHVLYVISDLPKLFSWLVNFKKSGNNPSRNTLVKAFTEVEFFNLYSKFLQDELRHKNQYRKLLLFYVSTSISALATLSSTGSDKLKVFVPFALECVSSLLASSNEESKISAYTILVVISAAVPLSREIVLASVETVLLSAPQSAKESAFNCALKLLQSVQDQGLEPLPKKIVDLLPEGLLSDTSIPYLDFLSTTKNSGKFICAYFRSLIINNVSVDSAVLKNLETANIELSNQQFTLIAVEVLKSVESKNSSSYIDLLKFLIRKNRTLFFDILKTKKLALTNLEMMVQSTLVDQTTEASLETDDETINIKNTHSKGVDENVIQTYEKHQALVASFLSNDSEVGKSYDFYENLYLKSMLDNSTASFLEICLVKPSLIISFLLRVATSYCPLKARIQAITRLIEAINNLDPDVFVGPILPVVITLLVDNVAQIRRRASELISSLNDKVKKELSPKTILLNDNLYGDKSSDIAVISPKDAKLLLETLEANIPNFIVDSTALFSCLETLLTNKKLGKLYLAFFTTHALYVDIPVIKMNLIKISVKSASNVRGAAAPSSLFQDILSSYVSNRDDWILKCSQTNCDSHAFESNIVSLVSPKEKNDSAITFLENALASPSEQLSNLAQTRIIEIFSTLKFDFQIKIVNYILEEHLTDNFVTYDPIDVLESINMTNEIFTELLKNSALNTTNSQAQSNPPKRRRRSSQSTRQALKDDEVSNMATSHLKKVTMLLEVLDYFSKQCEFEPSFELLKLIFVVLDDLETLGKDGKLPILYAQETIASCLKNIIEKLKEMGVPIKDASSIRADVVVSAIRASDSPQVQNKLLLVIAALASLSPELVLHSVMPIFTFMGAHTIRQDDEFSGHVVEQTIICVVPALANAAQYGKIDEIEFLLASFVSAFLHVPRHRRVRLFTTLARTLGGDLSIHLILFLCGQQYVNAYMKHRMGDCSALVDFATVFLQVFSAKEELDATRRFLDLWKCIPMEPIEKDSPTFKELSSKVIFGPSIVSMSKSELYHWRKGLVSFIRHALTDSQGTNGVPKLRLKIASQILEDRNTNDLLSSFSETIKYLLDVVDMLSRNHEDDEIMNKMYKLLGDVLNLLPIQYYSQSVNEILKSPEVSLQTAKNLVTLTAFKFNLEHTENPYAHESITSLMPTILEKITLQADLELSQASLDTMASIFHRFGSHIESSSLIKFLSVISGKCGLVNEDSPELTISSINCITSIVVIVGVKMIGLFPKIVPPIFKIFDKYVENKQESSKLIQISIIVFFSTLVKKIPNFLTPNIKDILKTLFKAHAVSDSIRSNVLDVVVEYIDSKILLSSLCSLWTFVSELNVTAVGLFIGTMENTVEKMNKTTAISESTMFFRFLTNALEYRATSTFDANTIGRVESMIHNCCIVYVMKLNDKSFRPLLAMIVRWAFDGEGTISNIGEVERLESFYKLFNKLQENLKAIVTTYFNYIIDSTVALLERLASGEVISISLRRLVLISLTSSFKYDQTEYWQVGSRFDAISEALTSQLSNIEDPIGKHLVKCLCALAQDISSSDDHNKRLNELIISHMRVIGDVEPSAREKYWSIKALTTIYKRVGEAWLSLLPQLVPIVAELLEDDDDDVQAAVREGLAKIMEELMGESLEHLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRF1</accession>
    <name>A0A1E3NRF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_27166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019775.1">
        <property type="nucleotide sequence ID" value="XM_019162483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRF1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRF1"/>
    <dbReference type="GeneID" id="30179170"/>
    <dbReference type="OrthoDB" id="426235at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033883">
        <property type="term" value="F:pyridoxal phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43885">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43885:SF1">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13290)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22390" checksum="40EEFF5CE3AB2A92" modified="2017-01-18" version="1" fragment="single">PLRGVIFDMDGTLTRPQTWMFGEMRRQLQVPAGVDILDHVASLATVEAQTEAEAKLQAIEEKAMREQEPTKGLQRAFAMLSERGVYTSICTRNVAKPVGDFCGKFLQCDPFDGDGRSRIVTGPVVTREFKPPKPAPEPLLHIISAWGVAPQQVLMVGDSRDDMDAGLAAGCAVVLLRHEDNSHVETAYLEKNASSKLDAVVSDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRI0</accession>
    <name>A0A1E3NRI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoglycerate mutase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_85646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019799.1">
        <property type="nucleotide sequence ID" value="XM_019165017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRI0"/>
    <dbReference type="GeneID" id="30181704"/>
    <dbReference type="OrthoDB" id="3898179at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF27">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="698"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="771" mass="85205" checksum="2DCFB07BC8D5B5F1" modified="2017-01-18" version="1">MHIVLIRHCTRIDKSKHGISPGQSEVSLHAGDQNDNKVGVEDYDESSNWLSNFDPPLNEGIASLEMESAFKKISNDILSKSRTKQIMIHSSPYNRCIQTSELLLDKIIHGKQNDVEKINTKLRVDQALSEWLNENYNLKYLPPNDDGYSMINNVNAYLNQPSLSASNGAFTETTKNQLRNVKDSTWSYNQLGHCGEYGESASAFTRRCFNYLINLLQYYYTKQGSEVDRQMAVVIISHGAVISTLLQILLGRSIFSEIPLCTPIYFKQSQKRRSVFKLMDYDFNLNKLLGPSTDQEFYKILDSPIDLTKLDPDNLRSELTIGTTGFTTIIQSIPKQIGSPKQSGGKGEPTRRRRNTINIGDKEKDASEDNKIESLKQTRSSRQLYLLNKNTSEEKVIDLDKLHSYFGGDSGSDTNSEDETGADGGRSASFDEFKDLQENGLNKSVYKGSITSLSSFNEENKSKFQLNMKNFFSRPSPIKGDPFSHFFLSRSSTMDDDGSDYDEFNMSRKSSVTNGDDSIRLSAFADNVAAASSISHGLGVFAGDDDAESSGDDSDGSDTRILSFGTRNKLQNMILTERAKEKEKDARARMQADEPTTVLDGTFAKVPLGSNSKLSLAAQGAASASSSTGDIKGSGSGSGADGGQGSKIKLVMPRLISTRNMNLHVRGADGDGDGDGDAGVGGSSAHETEYSAREERQRRRQQQQRRHRPSRGSVSSEDTLKKILLQEDLRPGWPVRSLGADPPAGDDADENDAEEEEEEEETGWFGGFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRM5</accession>
    <name>A0A1E3NRM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_70336</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019832.1">
        <property type="nucleotide sequence ID" value="XM_019163614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRM5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRM5"/>
    <dbReference type="GeneID" id="30180301"/>
    <dbReference type="OrthoDB" id="2449818at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000006">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000002">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000009">
        <property type="entry name" value="Ribosomal protein L10a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="217" mass="24518" checksum="875CDF96745C4C52" modified="2017-01-18" version="1">MSKINAVQVRENVQELLKYSTETKKRNFLETVELQVGLKNYDPQRDKRFSGTLRLPQVPRPNMTICIFGDAFDVDRAKGLGCDAMSVDDLKKLNKNKKLIKKLAKKYNAFIASETLIKQVPRLLGPQLSKAGKFPTPVSHSDDLYQKVVDQKSTIKFQLKKVLCLAVAVGHVEMEEDALVSQIMMSINFLVSLLKKNWQNVGSLVIKSSMGPAFRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRN9</accession>
    <name>A0A1E3NRN9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019859.1">
        <property type="nucleotide sequence ID" value="XM_019160304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRN9"/>
    <dbReference type="GeneID" id="30176991"/>
    <dbReference type="OrthoDB" id="4009808at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="289" mass="33004" checksum="AEB8EB5E403ED3ED" modified="2017-01-18" version="1">MPIVESPAIYVQSVLTTIATYFIQHAYVKPIIDLHSTDANFIPLDKPTKALHIRRDNSSRQTKVNVFDNKGDKLYTIERESASTPTWSMFTYPHRHEVATIRAGFFLKSFDFHNKFGMQHRVLERENGLSGRLRTFYLNDGYKYVWTRGSKFLERVTNPGGNEEETRERIAKVRLMRQRKFDYELVVDENKVDLEVAIVTGFLCMMTFWGMGDITETVGPTYIPEPQAAPEVKAVEPVAAKQEPVIIEKVIERIIEKAPAAPEATKEPIPNITLEVQADDDADVIIEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV7</accession>
    <name>A0A1E3NRV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Porphobilinogen deaminase</fullName>
            <ecNumber evidence="4">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hydroxymethylbilane synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_27099</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019933.1">
        <property type="nucleotide sequence ID" value="XM_019162476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV7"/>
    <dbReference type="GeneID" id="30179163"/>
    <dbReference type="OrthoDB" id="564646at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13645">
        <property type="entry name" value="PBP2_HuPBGD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.40:FF:000002">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="12">
        <location>
            <begin position="232"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="35785" checksum="9A63119CE6D3745E" modified="2017-01-18" version="1" fragment="single">VRIGSRKSLLAVRQSEIVGEAINRFYPNLSINISKVFTLGDQVQNRPLYSFGGKALWTKELEILLLQDVGEYQKIDLIAHSLKDMPTILPEEFELGCIISREDPRDALVMKKDSPYKSLTDLPAGSIVGTSSVRRSAQINKNYPHLTFKSVRGNIHTRLSKLDAPDSDYACIILAAAGLIRCNLDDRITKFLNCNEMYHAVGQGAIGVEIQKNNEKMRLLCSVIGCRTTTWKCVAERSLLRRLEGGCSVPVGVWSTLEDNNVLRLKAIVLSVDGKESVEDVHEKVMACDKDALDLGLELAEKLIAKGAKKILDEINFSKVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRW6</accession>
    <name>A0A1E3NRW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019939.1">
        <property type="nucleotide sequence ID" value="XM_019160333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRW6"/>
    <dbReference type="STRING" id="763406.A0A1E3NRW6"/>
    <dbReference type="GeneID" id="30177020"/>
    <dbReference type="OrthoDB" id="432299at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000001">
        <property type="entry name" value="Cytochrome b5 reductase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="100"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="177" mass="19998" checksum="CB35AFFFD511E02C" modified="2017-01-18" version="1">MSHTKVPLINEPEKEPEKASAPANGSKTLRFSVPARSSKAAQHERLFPAPNSPQVIQPLSATPAASRREKVRLAPHHSALDWERHKHEKDVKHIDPSCFPVRITKEELSKHTSPHDCWVALGGKIYNISSYLDYHPGGVGILVKNAGKDCTALFMKYHRWVNFERILDECFIGFMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSC3</accession>
    <name>A0A1E3NSC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Mre11 DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_29427</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020170.1">
        <property type="nucleotide sequence ID" value="XM_019162592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSC3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSC3"/>
    <dbReference type="GeneID" id="30179279"/>
    <dbReference type="OrthoDB" id="30417at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010791">
        <property type="term" value="P:DNA double-strand break processing involved in repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010780">
        <property type="term" value="P:meiotic DNA double-strand break formation involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062176">
        <property type="term" value="P:R-loop processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003701">
        <property type="entry name" value="Mre11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00583">
        <property type="entry name" value="mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 16" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 16" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 16" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="17">
        <location>
            <begin position="298"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ49057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="415" mass="46934" checksum="7BEE448E01AF2FE3" modified="2017-01-18" version="1" fragment="single">MPEVSHIEPGPDTFRILLTTDNHVGYMENDQIRGDDSWRTFAEILDIAREQDVDMIVQGGDLFHVNAPSKKSYYHVMRTLREHCWCDKPIEYKLVSDPSDAMATKHFMYPAEYDSNVNVGMPLYAISGNHDDATGEELLSPLDVLSVAGLLNHYGRVIDNEQITVCPLLFNKGETNLALYGLHSVREERLMKTMASGNLEFLEPDTSADPGIQWFNLMCIHQNHVHRPGVKVVEETCLPSFLDFVLWGHEHDCHPSAVRNDITGAYILQAGSSIATSLSEGETLDKNVFVLSVKGKDFSLQPIRLKSVRPFVMKDVVLSQTGLSATSSNKKEVLNFLIMQVELMIGKANEIWKHNNRKLFEDGLMGESDAPLPLIRLRVEYSGGYEVENPRFFSNRFVGKVANINDVVLYYKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSC6</accession>
    <name>A0A1E3NSC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-dihydrouridine(47) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="5">1.3.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21 22">mRNA-dihydrouridine synthase DUS3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">tRNA-dihydrouridine synthase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">PICMEDRAFT_9475</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs. Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>5,6-dihydrouridine(47) in tRNA + NAD(+) = uridine(47) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53364"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:53366"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>5,6-dihydrouridine(47) in tRNA + NADP(+) = uridine(47) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53360"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="27">
            <dbReference type="Rhea" id="RHEA:53362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Dus family. Dus3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.89" evidence="5"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020085.1">
        <property type="nucleotide sequence ID" value="XM_019165096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSC6"/>
    <dbReference type="STRING" id="763406.A0A1E3NSC6"/>
    <dbReference type="GeneID" id="30181783"/>
    <dbReference type="OrthoDB" id="259935at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102265">
        <property type="term" value="F:tRNA-dihydrouridine47 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000145">
        <property type="entry name" value="tRNA-dihydrouridine(47) synthase [NAD(P)(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25585">
        <property type="entry name" value="zf-CCCH_DUS3L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="18" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-0521">NADP</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="29">
        <location>
            <begin position="254"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="DUS-like FMN-binding" evidence="29">
        <location>
            <begin position="444"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="28">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00033779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="ODQ48972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="622" mass="70594" checksum="7581871C28D3560E" modified="2017-01-18" version="1">MSEVESNKRPAEEVTDVSIEVKKSKTDEVPKGVASIKPEYIISASDADAIFDDDAAEASTAVEQEEMEKSNNNNGKGKGKNKNRGQNKARKLVQVKDEMKICPSIMDPESGKQCLKGSECSWCHDVTAYLKQKEPCIEGACPVWKELGYCPMGIKCRWLKSHFNCDTNTLIYEQGYARDENKGEVNFVSLADKRLLHAKKFDFKTSEVALKMVDEDRVAKMDAEKRKALENEFNESKVKASEKKKLDYKNKKVLSPLTTVGNLPYRRLMKTLGADVTYSEMALANSLISGQNSEWALLKAHQSEYPGFGVQIAASKPWQAAKSAEAIYKFANKNVSEINLNCGCPIDLLFKKGEGSALMSSSNRMKNLLKLMNDCSGDLPITVKMRMGILDKKPVAIDIVEKLLNAGDVGAITIHGRSRQQRYTKEANWDYIEEVGKVVKTYNEKYEEMKDVSDRPNPVYLIGNGDCFTHEDWYEHTARQGVDTVMIARGALIKPWIFEEIEAQQYLDKSSTERLRIIEDYAKYALQHWGTDEFGINTSRRFLCEFLSFTYRYIPVGILEKLPPKLNQRPPKWRGRDDMETLLGSSDYKDWIKITEMFMGPANESFSFIPKHKSNSYETPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSI7</accession>
    <name>A0A1E3NSI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_30003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019751.1">
        <property type="nucleotide sequence ID" value="XM_019162620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSI7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSI7"/>
    <dbReference type="GeneID" id="30179307"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="15"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="104" mass="12138" checksum="822F08F4CF4D2AF8" modified="2017-01-18" version="1">MPCAADLVKQHRFLMLSKSWCPDCHYAINIFKKYNVLDKVHLVELDKMNTVESLKLEKEFTAISGRKWVPSLFFNEKFWGNEASLKKLESLDQTEAEFKKVGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSN5</accession>
    <name>A0A1E3NSN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sas10 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_14519</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020129.1">
        <property type="nucleotide sequence ID" value="XM_019160410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSN5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSN5"/>
    <dbReference type="GeneID" id="30177097"/>
    <dbReference type="OrthoDB" id="1924577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018972">
        <property type="entry name" value="Sas10_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF8">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09368">
        <property type="entry name" value="Sas10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sas10 C-terminal" evidence="4">
        <location>
            <begin position="77"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="151" mass="16811" checksum="26C733228A4795E1" modified="2017-01-18" version="1">MAVVTYTREAAAEITPAAQKKAPATSHSPEELEAFLNSTQKEKEERKLARADAHKLATLAARDGKLMELQREESVGESGKRAIGYQIMKNKGLTAKRKKDNRNARVKKRKKYDIAKKKLKSVRAVYTGQQGPYVGELTGISKKISRSVKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSR3</accession>
    <name>A0A1E3NSR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MED9</name>
        <name evidence="9" type="ORF">PICMEDRAFT_70659</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020197.1">
        <property type="nucleotide sequence ID" value="XM_019163700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSR3"/>
    <dbReference type="GeneID" id="30180387"/>
    <dbReference type="OrthoDB" id="3991607at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011425">
        <property type="entry name" value="Med9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07544">
        <property type="entry name" value="Med9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="284" mass="30581" checksum="DBB8CCCC5205DC00" modified="2017-01-18" version="1">MESNVQAASQSFSASTVGNGSIPPSDESKVPPLASTTDAPAVQASPQIRGQSLSPVSISGSDSAVVSSAVSESLETVKANQEVINSAQDVPPPPSSVALLNIDDVFDKDQVRNSVSENGNRENQGQDKMDVDGPEPDGKPNAEQIDKADGESKDSSSNKARDDDVDFTDDDSERLNKIQSIHETLEKMENVRLELLPLLLDIIEQVKNGELSIKDVDNACGRIKVRINRLKESRNTVESGLQQLVDESNGTEHSQHETEERIKAKSDAISRLIADVNARNVNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NST8</accession>
    <name>A0A1E3NST8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_28851</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020233.1">
        <property type="nucleotide sequence ID" value="XM_019162560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NST8"/>
    <dbReference type="STRING" id="763406.A0A1E3NST8"/>
    <dbReference type="GeneID" id="30179247"/>
    <dbReference type="OrthoDB" id="10260443at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015169">
        <property type="term" value="F:glycerol-3-phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000185">
        <property type="term" value="P:regulation of phosphate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14478">
        <property type="entry name" value="SPX_PHO87_PHO90_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF92">
        <property type="entry name" value="LOW-AFFINITY PHOSPHATE TRANSPORTER PHO91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="565"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="635"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="704"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="745"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="787"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="7">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="816" mass="90566" checksum="32EEFAA8043BEAFD" modified="2017-01-18" version="1">MKFSHSLQFNAVPEWSSKYINYSTLKKIAYNLQKQYVQELNSKNLNEEASFTSDPNDPENPVVVFKQALDAEADRINKFYNQKEEEISKEYISVFHEFSDFESEFPYDSNDAKSRDQLKKILRSLLPNYQDINEPADHLSNHDENGLPHESHMESVDDLDSLRLQKKKSFPTLFYDDDFHVTFLEALKVEKKPLITRVFTHISELKSFIELNKIGFGKALKKFDKLLQTNIKSEYLSKLENDYYVFKNSTIEQLNAKISDLVTLYSIFTHHNINEAKLELGTNLREKVVFERNTVWRDMIGLERKSQAAYAKPEFAKEGAGSHGNPFTIKLGAGRQLTIPGFLTSLSTLKIVIIALITLVLLNISPFEDEQQKNCFAVLICSSLSWATEAIPLFTTSLLLPFLLVVLKVLKNPETGEVFSAPEASKFICAAMWSPIILLLLGGFTLAAALSKHNLDKYACTFLLSKVQQRPAPILFAIMVVSTIIAAFVSNVAAPVLMYSVVQPILNTLTDKTVNGKTVQNEFAQALVLGIALASSVAGMASPIASPQNVVALQFMDPPPNWIQWFSVSIPVCACCLVLIFIVLMISFSFKDVTIVPIHQHSDNFSSKQYFVLAISLVTIVLWCLASVLEPWLGDMGQIALISMVTFYATGALSQEDFNNYPWTIVILAMGGLALGKAVTVSGLLTTIANLIQKQLEDAELFTILAVFGFIVLIMATFVSHTVAALIIIPLIAEIGRSLPDPHPNILVFCTSLICSCAMGLPTSGFPNVTAIGLTNNLGDKYLNVGTFIRIGVPTSLGCLLIVITVGYLCLRLIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSY5</accession>
    <name>A0A1E3NSY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_21130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020295.1">
        <property type="nucleotide sequence ID" value="XM_019162185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSY5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSY5"/>
    <dbReference type="GeneID" id="30178872"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF10">
        <property type="entry name" value="PHO85 CYCLIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="172" mass="19685" checksum="6FE82201C721F99E" modified="2017-01-18" version="1" fragment="single">DLEALYVFLNKPVSAEMVHYLVATTASIISYDYPSPPQSPQHSATPSKRRPSLYSFIHRLIQHSHVQSTTLMTCLIYLHRLKQVIPPNSVGMSTTHHRIFLGAMLLAAKYTNDSSPTNKHWTTYTDGLLSLREVNALEIEMIQYIGWGNLRFENSDLIHSLSYFLEPIKRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT31</accession>
    <name>A0A1E3NT31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019836.1">
        <property type="nucleotide sequence ID" value="XM_019163615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT31"/>
    <dbReference type="STRING" id="763406.A0A1E3NT31"/>
    <dbReference type="GeneID" id="30180302"/>
    <dbReference type="OrthoDB" id="166134at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15737">
        <property type="entry name" value="FYVE2_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021565">
        <property type="entry name" value="Rbsn_Rab-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036531">
        <property type="entry name" value="Rbsn_Rab-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164:SF29">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11464">
        <property type="entry name" value="Rbsn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140125">
        <property type="entry name" value="Rabenosyn-5 Rab-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="277"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="554" mass="63594" checksum="D034AFEF06A69D23" modified="2017-01-18" version="1">MSKRRILGKSDGLPGTPRPATFLAQNGQAKSQSDNFICPMCNLPMKSLTQLNHHVDSIHLNIPTHNEPLDSEIGKSLERRTTSSSPPGSTISITSSGQKQHKRVDSKGTTSSRSTTISRGPLRLPTGHFVRPSNGMKCSDPRCHHKIGLKHGTINCSKCGRIYCSEHCHIPLKLNANLEVVDSNSLEGIWSKCCLQCLNDYDGWSGVIGNVPLVDKTNDFIRMRKTKIEDESLRNLVLEKRVEKVFSWIVNTFNSSGKFSETELRVFEAQLADWQDGHSVSECPICHTAFGFFIRKHHCRICGDIVCGDLRKGCSMVVPLGIIFDLMKPDENLNSKNQEIEYEKIEHFLKKDMFGVRVCSNCKRRVFNKKVFEVDKLKSENGDFMRSYKMWKMISKKIQTENMTDIKNDEENAMLVRLFTRLDKLAKQIQKIKSESQLSIDELKMYNTLESAIVNYVQAKLPILRKAQEEKLTREREVLQELIDGKPKLSMRDLREKREKLMVLNEQKYVVESMYQDFKKQRRFDDLKALDSNLEDIEREIQQLTEELGDDAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTA4</accession>
    <name>A0A1E3NTA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="2">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_70880</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020444.1">
        <property type="nucleotide sequence ID" value="XM_019163760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTA4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTA4"/>
    <dbReference type="GeneID" id="30180447"/>
    <dbReference type="OrthoDB" id="409189at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070981">
        <property type="term" value="P:L-asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000031">
        <property type="entry name" value="Asparagine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000050">
        <property type="entry name" value="Asparagine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006949">
        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="14">
        <location>
            <begin position="2"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="365"/>
            <end position="366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="13">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ49331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="572" mass="64080" checksum="02DFC41B80D78928" modified="2017-01-18" version="1">MCGIFAAYNVDDVLAFKPKALQYSKRIRHRGPDWSGNVIANSTILCHERLAIVGLDAGAQPIVSPDGRYSLSVNGEIYNHIELRTVLKDYDFKTLSDCETIIPLFEKYGVDAPNYLDGMFAWVLYDKETDRIVAARDPIGITTLYMGRSSKNPKTVYFASELKCLTDECDQIEAFPPGHIYDSNTDKMTRYFKPNWWDENKTATGDCDFTAIRESLELAVRKRLMAEVPYGVLLSGGLDSSLIASIAVRETQKAAAAEANVAIDANKTLTGVDAKGNLSFTGWTKLHSFAIGLPNAPDLIAAKKVAEYLGTIHHEHTFTLDDGFDALSDVIYHLETYDVTTIRASTPMYLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTESVKRVKNLHLADCLRANKSTMAWGLEARVPFLDKEFLNVCMNIDPKYKMINSKEGKIEKYILRKAFDTSDDPSAKPYLPKEILWRQKEQFSDGVGYSWIDGLKDLAEKQVSDEEFANPKPEWGSDIPTTKEAYLYRCKFDELFPSKAAASTVMRWIPKAEWGCHEDPSGRYAKIHEAAVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTD3</accession>
    <name>A0A1E3NTD3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_29294</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020031.1">
        <property type="nucleotide sequence ID" value="XM_019162585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTD3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTD3"/>
    <dbReference type="GeneID" id="30179272"/>
    <dbReference type="OrthoDB" id="25408at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019488">
        <property type="entry name" value="Nucl_pore_RNA_shuttling_Mtr2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10429">
        <property type="entry name" value="Mtr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20374" checksum="3B26C36D529B34BC" modified="2017-01-18" version="1">MGGFPITSTSPPQSDTSAVVEQFLSKILETLDGSIPNPQSITQILPQLHQHPRIIIDGNPFGSKEQFQQLWATLPLSTHQVASCDVHFIPSSDGQQVNPNQGQFIVLAHLKVRFDESGRNRVGETALFNSAPTSSTRAIWSHWFGVSLSFVVDSTISNNFNTESISTWDYRFTESPVNSIFQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTM6</accession>
    <name>A0A1E3NTM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Membrane protein PTM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14884</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LU7TM family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020537.1">
        <property type="nucleotide sequence ID" value="XM_019160580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTM6"/>
    <dbReference type="STRING" id="763406.A0A1E3NTM6"/>
    <dbReference type="GeneID" id="30177267"/>
    <dbReference type="OrthoDB" id="19932at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053938">
        <property type="entry name" value="PTM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF1">
        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21902">
        <property type="entry name" value="PTM1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133515" description="Membrane protein PTM1" evidence="9">
        <location>
            <begin position="23"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="PTM1-like N-terminal" evidence="11">
        <location>
            <begin position="33"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="10">
        <location>
            <begin position="185"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="514"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="514"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="533"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="66124" checksum="71AF377A277C21E4" modified="2017-01-18" version="1">MRLAFGLYFLLYSFLAVSTVSAQKIKLTGKAAEYCTGMYSRIDWGGPTEPFIKVDLNSFDSTNPATGNASISLIIFEYKDIDQLGVTDAEGHRRYICEEKLVNEGLCTSDQLYQFIVEDNITTYASVKTAVLTDLGTNDFSYLVSETGYYCIAAFNPPYSGGLNNFDMTVNFHNAFGNLPASEIPLLSLYGLLAVVYAVCLCIYLFPVFRHRSELLLLQKYLAGFFVFLTIEDILTWSLYDVQNNNKKYPLPGGVQFYIVFISILNAFKISFSLFILLIISLGYGVVYPKLPRRLMNFCKIACSIHFLFSVGFAWLSYYTTQSQPTASTTDTSKASDTFDSDSWIILLITIPLACIFMGLYFWILSSLRKTTQTLAENKQIVKLKMYQKLFKLIFGSLLLLVFAFIVSSIIVFNDNLTESIERFWKFDDVLTTFWPACIYFIVFTGIAIIWRPTDTSYLLAASTQVPTSAGNAIPTADTVDLEQYDNDFEFDDLRSLESNINPFETRENHNITQSATENPFDDSNQLQDPFDDGQHDLDEQLKQEKEKLKSSGNTDNFELEDETDDEAQNQGSDFESNPKTKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUH8</accession>
    <name>A0A1E3NUH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_99107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020411.1">
        <property type="nucleotide sequence ID" value="XM_019165142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUH8"/>
    <dbReference type="GeneID" id="30181829"/>
    <dbReference type="OrthoDB" id="10662929at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133523" evidence="3">
        <location>
            <begin position="30"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="360" mass="39299" checksum="82BD4ECBF751346D" modified="2017-01-18" version="1">MCTFYPLVVRLSILLVIPLSDLAVSLSAATPCHSLALSNGLSLLQYWLYAHHYYTTDKKLSLFSCCSFFIYAVSLLSQLRFRRRSADRSSLGGSSALFSSSAQDIPSRPHSGSSLINNFTNSLSNSISISPALTSLSKRSTDLLLFKIDDILSPHMQSSQQQIPARSDLHPITTKEKGPAYNSYSPSLIEYLPTGRPSLRSSKTSPIMATLLALSAVPPSNAAPVVTLNSASAHNYNISPVLNFLLATSINLVSMHACSHCSTLHLFKSLVTGITWLLILLMGCPFTPALPSTVVILFENDFLILINTIIPAVSFILCALRFFSNRYTPEKGNDINIDNSHGTPSKQKNIVVSYRTIPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDR1</accession>
    <name>A0A1E3NDR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">low-specificity L-threonine aldolase</fullName>
            <ecNumber evidence="8">4.1.2.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_18507</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="8"/>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015380.1">
        <property type="nucleotide sequence ID" value="XM_019162034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDR1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDR1"/>
    <dbReference type="GeneID" id="30178721"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06502">
        <property type="entry name" value="TA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="11">
        <location>
            <begin position="14"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="369" mass="40402" checksum="E081D78DDFE0DDD5" modified="2017-01-18" version="1">MTEATSQLPENHNEFRSDTFTTPTQSMISSIGLASVSDSVYNEDADTKALEKKVADLMGFEAGLFCMSGTLSNQIALRSHLKQPPHSVLCDYRGHVYVHEAGGLATLSQAMVTDIIPSNGLYLTLEDIVARYIPDDGDIHAAPTKVVSLENTIHGLIYPIEEIKRISEWCRANGLSIHLDGARIWDANIATGVPFTEYGKYFDSISVCLSKGLGAPIGSVLVGSADLIKKANHFKKQNGGGIRQCGMLCRMASIAIDENFSKMKQSHVMAREVADYAESIGLVLEIPCQTNFVFLDPLKNHIDPALISVVGEKYDIKIAIFRLSFSFQNSRVAVDNLKAALKELKEEAERNPYHSNKAIGMYNTGNGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDU1</accession>
    <name>A0A1E3NDU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_22620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015403.1">
        <property type="nucleotide sequence ID" value="XM_019162271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDU1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDU1"/>
    <dbReference type="GeneID" id="30178958"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000221">
        <property type="term" value="P:negative regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000030">
        <property type="entry name" value="Forkhead box protein K2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF1">
        <property type="entry name" value="FORK HEAD PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="64"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="238"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="238"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="39916" checksum="2E3BFFA636CB6D78" modified="2017-01-18" version="1" fragment="single">STTVTEDSADDVNFVIRHLSTPSDNLPTYVSKQYNNDSIMGNEIQAYAKIAGCNWTYYVKSLTISIGRNTDFHAAGNTNNNSATASKDLIDIDLGPSKVVSRKHATIEYNLNGRKWQLFINGRNGLKVNGMRLNLSTNTPYDLSSGNIIDIGGTQMMFVLPDAPPVIPDYFRHLLSNKRLKMNTMPSSKGNSVDFNSKSDMVNTQVKAFQLQEGLSSLNPHSASSEQDYSKDDAKDLKPPYSYATMITQAILSNPQGILSLAEIYNWISSHFAYYRHTKQGWQNSIRHNLSLNKAFEKVPRKPNEPGKGMKWQISESYRNEFLKKWQDGSLNKVRRGTSVSRQLQLHLIKNNALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDY7</accession>
    <name>A0A1E3NDY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015460.1">
        <property type="nucleotide sequence ID" value="XM_019161928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDY7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDY7"/>
    <dbReference type="GeneID" id="30178615"/>
    <dbReference type="OrthoDB" id="5572108at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="796"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="809"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="826"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="948" mass="104110" checksum="525E1689767CA042" modified="2017-01-18" version="1">MPPAKRGSLATDPGILLKRRKDRQKANERLQLRLDEQGVKRTEVENNLSFSSIPQVLLINQKNYYTEYLKKDDNLRIVRNTREKINKKLKVKAPSATTKKGDLDRVKNGSASVSVSASANVTENENDDDDDDADDDADDAAEEDEGRTLVIHPGSHNIKIGWAEDVDPVLVPNLIGYVRREVSERAAGVTSASGASGANSKRSAQDVSGLDPPRYLARGDGEPVNADEDEDEHDDGDRFYVLNDDKEFRAKRKPINTSFRERMKYYKRRILPNCHEACFNFNSKQQKHPPAVDDISEDVSLSSSAVNMFIRASRWLKTAGRNYVVGDEVTRLADEKEWVIRSPFLFSSAGGTSGNSKRSSVELGFNEQDVNYSSREEILGDIETMLVHTILHKFKVAKKSDLKKINVVLVIPSLYRKSYVESMMELLLTRMNFKNATLIQEGMSASFGLGLSTGCIVDIGATSIHICCVDDGAILEDTRVSLDYGMADVVRFWGKNLIAQQFPYAGVDFRQVKDWKLIEGTFADHGTFDDSKVAIQVGKFSVYESNTRVRRYQFKCFDEGVVCPMGVFYPEIFLEETAAAAAAAAAAAAAAAAANDDVDARQKERIVLGSIAPAKRVHEPLFDGRSNKFTGYSNSDPISILQDLQRTGVKLSELAVEPLLDLLVELSDLFINSSAIGSGRRGKTNDLSSKLKSRYRNAHYAQQSAAADRRPTTDGMRQNMTPLDRAIIESITVAGLGDQTRLEKMYTNICLVGGGGRLDGFDSMLMDRLHINRNEILSSTKLVEALGLIKQWRREYDKEHKPRPKDAAGGDTAAPAGEKSPAAKTPADKPDKTPADDDDEDGPADPAPAPAAGEDDDDDAAAAAAAAAGEDELRLSKEQLRTVTQLLLAGAPMAVDITGRGELDPLVLGWKGGCVYARLKIFEETCVSGADWDMLGSRALTYGSLFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEQ1</accession>
    <name>A0A1E3NEQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome c oxidase assembly factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_36618</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Required for assembly of cytochrome c oxidase (complex IV).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Component of 250-400 kDa complexes called cytochrome oxidase assembly intermediates or COA complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the COA3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015740.1">
        <property type="nucleotide sequence ID" value="XM_019163021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEQ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NEQ1"/>
    <dbReference type="GeneID" id="30179708"/>
    <dbReference type="OrthoDB" id="3995942at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041752">
        <property type="entry name" value="Coa3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018628">
        <property type="entry name" value="Coa3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15642:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 3 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15642">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09813">
        <property type="entry name" value="Coa3_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c oxidase assembly factor 3 mitochondrial coiled-coil" evidence="10">
        <location>
            <begin position="19"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="85" mass="9942" checksum="9E4A5687922A293F" modified="2017-01-18" version="1">MLSNNGYYEPHTYRMSPALLRARQPYFVKNMIGLAILIAVPVGIYTYTYNFLNQDDFDDIPIPPLDEDTIHQLQKEYEEEKKSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NET2</accession>
    <name>A0A1E3NET2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015754.1">
        <property type="nucleotide sequence ID" value="XM_019161858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NET2"/>
    <dbReference type="STRING" id="763406.A0A1E3NET2"/>
    <dbReference type="GeneID" id="30178545"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="296" mass="32721" checksum="F79D6997A5F56FA1" modified="2017-01-18" version="1">MSESKKTALITGTSSGIGHYLALEFAKRGYFVFACSRKAESMADICAEYPGQIFAFSMDVSSLESIKAGYKFVSEKLKDLNIAGLDVVYNNAGSSCTFPGVDVPDAALVQCMDTNFIGPVRVVHEFSRLVIAKEGTFAFTGSCSGYCPFPWGSVYGASKAAIGQYASILAFELEPFNVAVKNFITGGVHTDIADKRPLPADSIYNIEPMKHAFEQRQKMSERNNPMEPSIYAKKAIDAIEYGKKSTVDVYLGTWSILPHLVAIFPRSVILYFFRLKFYLTDVWKALAEKHRNKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEX3</accession>
    <name>A0A1E3NEX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP synthase subunit K, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015785.1">
        <property type="nucleotide sequence ID" value="XM_019161868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEX3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEX3"/>
    <dbReference type="GeneID" id="30178555"/>
    <dbReference type="OrthoDB" id="2094445at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021278">
        <property type="entry name" value="ATP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28074">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28074:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11022">
        <property type="entry name" value="ATP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="6723" checksum="A2B602A62755F2F5" modified="2017-01-18" version="1">MGAAYNILGKAVPPHQLALGTIGAVVLLVLPKPWATTPKKEAKIDAANADEEKFIKAYLEKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEZ9</accession>
    <name>A0A1E3NEZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_13335</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015779.1">
        <property type="nucleotide sequence ID" value="XM_019160045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEZ9"/>
    <dbReference type="GeneID" id="30176732"/>
    <dbReference type="OrthoDB" id="343623at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031632">
        <property type="entry name" value="SVIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15811">
        <property type="entry name" value="SVIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="409"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="223"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="271"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="546" mass="57379" checksum="3F6E2F2BB437A387" modified="2017-01-18" version="1">MTSNQETMDPSLSMLSAMVAHSRNGSSGEANDSSDEIHNHGNEQDVNGTAEPDGNRVGEDGSGGGEGMDAEMKGDHSTGLGNDGNIDGSLPGTHPGQTGAGEGVTEKKKRGRPSKQTQPQSQSQEQLLDLSGGDTLQPPNGIVKRGRGRPRKYPIELRPSRPKLPPLYDENGNVVKRKRGRPRKNPEESGLNSDGTYKEGYPKHQQHQQQQHANGSQRDDKAGNGSHGSQSMSGSGSANNKLPSLSNLPNLPNLSDLPDLPRMSGISSLPNMNSNDLSNMNGMNGMSGMGNMNGMNGMSGMNGMNGMNGINMTGLNMAGLNGMNMAGLNMGAMNGVNGMNLNGMSGMSMNLNDMSGISGMPGMNGMNPYGGNMHNSSVSGLSLEPITLHAIQSVIASASRCMPLPVRQLKQPVNKTKRTAPQQTAAAATKGNTVAKAERQASAAKKSGIKSETRRLGTKSADEPPPEAVPVSVPVPAATTPPAEPEPVSVGRSSTNTAREMAARAAESRLQQQKKGQLGKKLDAERRKNNKDFAMEEYAAKTKPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF63</accession>
    <name>A0A1E3NF63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_21870</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015877.1">
        <property type="nucleotide sequence ID" value="XM_019162229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF63"/>
    <dbReference type="STRING" id="763406.A0A1E3NF63"/>
    <dbReference type="GeneID" id="30178916"/>
    <dbReference type="OrthoDB" id="17927at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21673">
        <property type="entry name" value="SMP_Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03105">
        <property type="entry name" value="Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027536">
        <property type="entry name" value="MDM34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058825">
        <property type="entry name" value="MDM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26545">
        <property type="entry name" value="Mdm34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="10">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="340" mass="39412" checksum="252050D38CB1A9DD" modified="2017-01-18" version="1" fragment="single">MSFKLNWNAIEKDSFLSYARELLEEALNSGKRPAILSDDIRIVALNFGTIAPIFQILEIGDLGVDKFRGIFNFKYHGDASITVTTKISASLLKNYNNNIDNDFDFIKPNFIVSDCDFDIPLNLTLSNFKMSSIMIIVFSKTKGLTLVFKNDPLENIDVNSTFDRITPIANFLQKKIESQISDLFKEFLPSLLYKFSLKYTTQSFDQFHRDLLIEEQEEDDNRRKNKILLKELDPENPFRISPGSLMRSTRLSSMRQTLSLGNGIDKLSCDRFNKDLITKAFMNILINKSKSYNTNRIELSDKDFEFGDVHDKLNFIRNFQNRAYSRNVSHKPKRRTIKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF77</accession>
    <name>A0A1E3NF77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015341.1">
        <property type="nucleotide sequence ID" value="XM_019162015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF77"/>
    <dbReference type="STRING" id="763406.A0A1E3NF77"/>
    <dbReference type="GeneID" id="30178702"/>
    <dbReference type="OrthoDB" id="3995946at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="550" mass="61942" checksum="86DED694F8E1EA91" modified="2017-01-18" version="1">MSVQATPQRQSTGNAPSSPRTPRRSLTPLTKHALQMSTTPIRNRLLLTPTKNKQKQVYDPLDDLRMLGRLFNTKARSKSRVVPITKNVSPSKTTTTSSYKTPSPVPEGNTFPLGTDVSTNLSDLKRRHVSSPQLSISPVLGMGNVPSFINKDVRSFAKLFHQSSSFSTNMQSLEMSDSSGFLEETLQITQSQSQSQSQSQSQHQSQPHMLLRIPYNDEVPSRNVGAGSDEIPDFEDNNYIDNYNETFESHADTSNIALPEAILRDGSGILGMSDDELEEYNLSLSKLNESGSDSRGDSDSDTENEGDANGNSEHVRGLVDTDELSKELSLIKSHEQFITKRKQRRKTGRMGEFTSGQRPIIKDAMFSNKMIKGLLDNLQLGDTIESGLDASLFNEMAYRFIDMELMKTIEMSELTGLGGRIVHTEVFYGDSEKQKEEDEEEGILLYAMHNWDMEKVKELEGVLYSNKAVRKRRADYNLLKRKELAHKKVQQEVEQNSEEINVEAEEDIPGDEVDLKNLDTHIMLRIANYSDDMDDSSDYEIPFAEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF86</accession>
    <name>A0A1E3NF86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015914.1">
        <property type="nucleotide sequence ID" value="XM_019161906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF86"/>
    <dbReference type="STRING" id="763406.A0A1E3NF86"/>
    <dbReference type="GeneID" id="30178593"/>
    <dbReference type="OrthoDB" id="5344169at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="618"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="704"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="519"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="720"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="734" mass="78999" checksum="ECC01569D56CCDEB" modified="2017-01-18" version="1">MDDQLDFQWNDIHQLDKMFSMATSGDADGSHGQNEDQDEDQDQDHGAADDGSTGNGSSTPDSHGHKHALPSGRESTAAADRNDRHPGSAATTATTSNLNPNLTDSTTPSSANNDYMLFLNLATPNSGIDDADGGPARSRDKDKERERDAAGRHSTKDWGKDSDLDHRPPTGSAEIGDGDPNGAGAFGRFSFDNMEASHLGYIVDQRHQHPQQQLLYSAVPNPQPPSTQTRSRINSVEFTPLLSPMINPSLERVNSNKGKSGFSPLSSPVLEFQKYNNTTSLRQKRKDPHDAHAASSSVKANSANSTYSDQPSGNGSGADQSGNLSLKFENSDARSYKRSKTPVSTPLLSTMDRKSKVSSNRNSSSTGSSTQTTPYQTYVFKQQQFPPSKENSMTLKPTSISASSNMDYPGNSFLDAFDGELTLPPPPTAGGKGLISENDGFVMPSAAFNSNITGNHNDNNELTMALNSASTIFNSSSANHSKKNSVLLNPNHHKSAQSSPVILPSSSIVLGSPRMEPKSLNARSSHASSASSSFRPTINTSKFANSSSSSLNTISNLSSSNVSAVGTNILGTPQINSLPTIPPSHSEDDGAETRSGSNAGNISNGEYQHHHHHYHHSDKKMSHKLAEQGRRNRMNIAIQDLEKMIPESMKRNITVPSKATTVELGCRYIQDLLHQLQNVKDGSSDGSFGVGEKLVKTESISPLDHLENSSNHLHHHHQHNSTNNNNNDFSDSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFA6</accession>
    <name>A0A1E3NFA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor 100 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18186</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015917.1">
        <property type="nucleotide sequence ID" value="XM_019161908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFA6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFA6"/>
    <dbReference type="GeneID" id="30178595"/>
    <dbReference type="OrthoDB" id="64353at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027075">
        <property type="entry name" value="CPSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025069">
        <property type="entry name" value="Cpsf2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922:SF1">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13299">
        <property type="entry name" value="CPSF100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="16"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Cleavage and polyadenylation specificity factor 2 C-terminal" evidence="4">
        <location>
            <begin position="821"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="581"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="614"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="895" mass="100934" checksum="7456E29A5485B961" modified="2017-01-18" version="1">MFEFTAIGDGSSQAKILSLDNTITILADPGWNGTDSLEFYAPYVNSIDMIILSQTTTSFIGAYAYLLHQHPQLRHIPTYSTLPVTKLGRVSVCELYRSLGLLGPVDGNSIEFNDVLRYFNSIKCLNYSQTIHLPHVNNKLINLSLTPYNSGYTIGGSIWLLENNINEKIVYAPIWNHSKDEFLNNCKIFNNSDLVRPTTFITNCEFVSSKYSHSTRVQTFMQLIEKTLYSGTNVLLPTTLAGRFFELIIPILLNNKISASIYMLNFTGLENLKILSNFLEWMNQSIIKLWENENQSEILFERNRIQLIKFEDLDSLVNTSEFQRKPKIFFVEEMNLMDGSFFSQFLIDMNSRLSYSMILTEKPNLNSKLHQFYKIWRQGVDATEADPKEGSHIVLEIPDVRLASVKEELLRGKELAAYLKKIGERKELELKLEKEEMEKRRLEEMLEAGVKVETDTEDEEDEEDADAEKVLANIDQNIKADESALSGNTLTKKDSQTAVGVEITANIIKTAEIKFQLDDSKRLKMDEILMLPRDFDVRSLKHKNRVFPFVNNRYNADDYGVVVKHEDFQIYDDDRFPILGADATNAHENGNDNKGSGDESGGSGMESDEEQGRGKKRNRRGGDRRRRKRRGGNRNRRNGGNDGNDGSGRRGKEENALYQPAVYSLESTVDPVVREKTTSRVTVRCGISFIDLGGNHDLRSLKFTAKQIKPRKVIILPGVSGNADDLMLELSEDTKQSILSGNDHSANIDVEYIKSKMNQKIDLGDVITSYELLLDEKLASSLHWQTLNDGYSVSSVGGVVEKLKDWEFKLTELGSTASSTMAVSSTKIGDVKLAQLRRALATKKHRVELMGDGRLVVDEELVVGKASEGNLSIEGGLGGLFYEVKDLIENMLATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFE9</accession>
    <name>A0A1E3NFE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_35923</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015965.1">
        <property type="nucleotide sequence ID" value="XM_019162975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NFE9"/>
    <dbReference type="GeneID" id="30179662"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF13">
        <property type="entry name" value="L-XYLULOSE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="275" mass="29793" checksum="ABDEBBC8B80F737D" modified="2017-01-18" version="1">MFKVRETPEITAPVSELFSLKGKTCVVTGGSSGIGSSVVEAFASMGATVIIVYNSTPVDQVVKDLTKKYNVTIDSYKCNLLEMSEIEGLFDNVIKKYSKIDVLVANAGIGWLHGGVNEFESYEQLVNNWEKFMKVDLSSIYYCCSFIGKIFEKQGFGSLILTASMSGSIINVPQLQTPYNVAKAGVKQMARSLAVEWSAIGDGKIRVNSVSPGYVRTKLTGKMDPALVEKWCKLTPLGRMASPKEISGAYVYLASDASSYTTGCDLVVDGGYSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFM7</accession>
    <name>A0A1E3NFM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_35735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016053.1">
        <property type="nucleotide sequence ID" value="XM_019162963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFM7"/>
    <dbReference type="STRING" id="763406.A0A1E3NFM7"/>
    <dbReference type="GeneID" id="30179650"/>
    <dbReference type="OrthoDB" id="73612at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013861">
        <property type="entry name" value="TMEM115/Pdh1/Rbl19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377">
        <property type="entry name" value="PLACENTAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08551">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01160">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39374" checksum="6156BB49D1EDB9F4" modified="2017-01-18" version="1">MESLSNQVSQFTSLPVITLVYLAITVTLSAITSYVRTQSYKHLIAGNPELVFEDIIIPSLQLVPAHFVFHPWTIWTSAFVETSPFQFISGLIIVYVGMTFLESQWNPKSSQQPLSEALKFTSIIIIISNLVCIVLITLSNLLYGSLSNLNLPLHYGLYILVLPFATVAKQLTPETNIRVLSLFKFRLKRLPFILLTLTLVISVIKGNAAPFLPAFVSFFTSWCYLRYFQFSPAINAFEVLPSPVSQAGSVNTPSRGDPSDTFALVEFFPDLAKPYVKPLFDGIYQLSVLLGLVRPWNDEEVDIGNIRSTFRVSGTQGITTSKPTSGDPISSDIDERRRQVALKVLEQSVDPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFV6</accession>
    <name>A0A1E3NFV6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_17469</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016079.1">
        <property type="nucleotide sequence ID" value="XM_019161617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFV6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFV6"/>
    <dbReference type="GeneID" id="30178304"/>
    <dbReference type="OrthoDB" id="10250970at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
        <property type="entry name" value="SF3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031781">
        <property type="entry name" value="SF3A2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205:SF0">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16835">
        <property type="entry name" value="SF3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="SF3A2" evidence="7">
        <location>
            <begin position="113"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="216" mass="25452" checksum="9051A5F21A7097EE" modified="2017-01-18" version="1">MDYQNRVGSKKGGGGVAGTAETNQYRRERLKNLLQSKINIESDPYVFKNHLGILECKLCLTTHMSESSYITHTTGRRHQLNLMRRAESERKQRSSEKSGTEEEIIEKRHWTTIGKPVYKVFKIRDPVSLNKGFIFTFKLDKIKADIKPLYRFMSCFEQKKESPNPKFQYLLISAEPYENVGFKIPGDTIDLRTDRIWDYWDPDTKEYCIQFFYLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG24</accession>
    <name>A0A1E3NG24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_73852</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016175.1">
        <property type="nucleotide sequence ID" value="XM_019164607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG24"/>
    <dbReference type="STRING" id="763406.A0A1E3NG24"/>
    <dbReference type="GeneID" id="30181294"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03753">
        <property type="entry name" value="proteasome_alpha_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000015">
        <property type="entry name" value="Proteasome subunit alpha type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033812">
        <property type="entry name" value="Proteasome_alpha_type_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="256" mass="28326" checksum="797D89BC9DD924B3" modified="2017-01-18" version="1">MFLTRSEYDRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGISTNEGVILSVEKRVSSSLLESDSIEKIMEIDSHVGCAMSGLTADARSMIDHARVASVQHDLYYNEPISVESLTQSVCDLALRFGEGASGEKRLMSRPFGVALLIAGYDDDKGPQLYHAEPSGTFYRYDAKAIGSGSEGAQTELQNEYHKSLTLKEAEVLSLKVLKQVMEEKLDSNNTQLASVTKEGGYRIYSDEEVAKIIELVKEQERDDQKMEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG54</accession>
    <name>A0A1E3NG54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_45965</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015661.1">
        <property type="nucleotide sequence ID" value="XM_019163123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG54"/>
    <dbReference type="STRING" id="763406.A0A1E3NG54"/>
    <dbReference type="GeneID" id="30179810"/>
    <dbReference type="OrthoDB" id="6608471at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071454">
        <property type="term" value="P:cellular response to anoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF6">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="327" mass="35314" checksum="2DB42BBCD7A8A2F4" modified="2017-01-18" version="1">MASSIPGPVPLPDSRYDLSTYIGRVKHCAEISDPTMLLSSKRDIEESKRLIWDYRNGVIPEMSPRLWRAKKILDSTIHPDTGETVFLPFRMSCCVLSNLVVTAGMLTPGLGNLGTAFWQIANQSLNVAVNTANANKSHPLTTKQLATSYFLAVGASCGVALGLNSVVPRLKNVSAGTKLILGRLVPFAAVVSAGVVNVFLMRGQEIKKGITVFDQDGNELGTSKKAAFQAVGETALSRVINATPIMVVPPLILIQLQKSVLKNKSLGVLTFANLGLIFSTSLVALPFALAVFPQRRFVHASSLEEKFHGLKDKNGAEIDTVWFNRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGB0</accession>
    <name>A0A1E3NGB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dihydroorotase</fullName>
            <ecNumber evidence="3">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18299</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="3"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015496.1">
        <property type="nucleotide sequence ID" value="XM_019161938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGB0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGB0"/>
    <dbReference type="GeneID" id="30178625"/>
    <dbReference type="OrthoDB" id="1670005at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00219">
        <property type="entry name" value="PyrC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001237">
        <property type="entry name" value="DHOdimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="8">
        <location>
            <begin position="88"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="361" mass="40205" checksum="65933714E5A9E505" modified="2017-01-18" version="1">MSRIELGHTADYHVHLRQGEMMELVAPTVREGGVSIAYIMPNLTPPLTDIDRVVQYQKDLQKLAPKTTFLMTFYLSKLLTPEIVAKAADENVIRGIKCYPAGVTTNSQAGVDPNDFSAFYPIFEVMQEKGLVLNLHGEKPSSHDSGEDIHVLNAELKFLPALAKLHKDFPELKIVLEHCTSKAAVDAIHELNKDLKPNEEPKVVASLTAHHLFLTIDDWAGNPVNFCKPVAKLPEDRRALLNAAVSGKPYFFFGSDSAPHDLSKKSTHVGVCAGVYTQRYAIGYVAEIFDKAGKLDKLKDFVSTFGNTFYCMDESKLVCKDKCVLVKKEVEIPDVIAFDDSKLKIAPFKPVEKLSWSTEWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGD7</accession>
    <name>A0A1E3NGD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Monopolar spindle protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016323.1">
        <property type="nucleotide sequence ID" value="XM_019161702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGD7"/>
    <dbReference type="GeneID" id="30178389"/>
    <dbReference type="OrthoDB" id="10465288at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="34831" checksum="7E7E900BADEC844A" modified="2017-01-18" version="1">MSSADVLISRAWMQVTSYSDSKLRLTDLLALFNVLEDLVREKLLSDEYRDFLIEFVMECPQVEVNQEQFKALMERLFECSMEDILRGGLTNKSKSRFAAVDVPLHGADYENREDFTQTMNMESSKFNSFMLKGTLREKEDILRGRIRELESMLTRSKTHNTRDSRTVNKMRDLLLEYYKSLDKITVSKSASSVPETGPMQGIVQRLKDGIDKQNVLINELKRKVGDDQPKSVFGQVRFRIVQIYMLVWRIVRLPLYIVLLLLVLNFLLYVFYDDGYAENPDVALAEEYWDSAAHVGSVNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGT4</accession>
    <name>A0A1E3NGT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA damage-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_60418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RSE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015891.1">
        <property type="nucleotide sequence ID" value="XM_019163436.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NGT4"/>
    <dbReference type="GeneID" id="30180123"/>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001143">
        <property type="entry name" value="Pre-mRNA-splicing factor rse-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="8">
        <location>
            <begin position="40"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="9">
        <location>
            <begin position="482"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="7">
        <location>
            <begin position="960"/>
            <end position="1297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1331" mass="148400" checksum="B86B397CD3D16DCA" modified="2017-01-18" version="1">MATADVIILQRVRFFKPLNINPTFSLYHRTLRQPSSGLSSIIGNFSGSRKSQEIVRATQTTLELWKFNKSTSEVSRLVVQNAYCNIRAIASFKVVKSTKDLILLTSDSGTLSIIEFDSVNNKFTAVRSETFYKTGVRRLSPGEYISVDPNGRAAMIGAIERNKLVYTFARDNSHSNGLVVMSPLEANRNHLLTYTMVAMDSGFENPIFAAIETDYSAQAPKIVKLLNYYEFDLGLNHVILSESETINSKSNYLVAIPGGYDGPSGVLVCSEGMIQYKCPFKPTHYAPIPTRGKDTSTKSIIVSSVVHKMKNAFFILLQNQFGDLFKVTLDYTTTQETKYDVIGNDVGPGMVTSIRIRYFDTIPICTSLLILKSGFLYADCEQGDQYIYQFEKLGNGSDEKEWNSEDYPDEQAVLEEENEGVAFDLKPFDNIRLVEANSSLSPMMDVKLISGDLVSSLPELYSITGSGPRSAIKVLHKSIPLSEIVTQELPSKIMKAFTCKIHKKDQYDRFIVLSFFDGSIILSIGEEVEEAENSGFIDTITTLDACQTGSDSIVQIHATGLKQIFYSEDDEPVKSIDWNPPPGIEVLFSSCTNTQVALALSNGDVVYFETSPTGDALVEFSLHKEFNASITSISLGDIPKGRLRSPFIIVTCKDASIHVLSTDPKETMKTKYTTKLYSTASSTAITSFSSNIEDYTDEDEDYDEMKDISLKRNSQIINFVHVGLQNGIYVRYILTDEGELIKPNMKYVMASPLMIEPIRVTNKVGESVTVAAIRSLLSFLVIPGSNEDIKLIPLPIAEKFVENENEDEDEEAEETTYSKTYGCVVPLHSADVPQGLILAHGEKLTIASLPLFNAVDDKHNGDCQTCVINKLKKLDNIESIPMRYTPRAVCDSVNNEYKMSYIACSDLDIKSMFNKPKESTEANKDVDEMVTYGNIEDNDAEEFEKLQNFGYSKSTDGDCASCIHVFSTENCAIGQTIELLGNQTAHRLGAATLEHDHKVGDYLLVSTIIKFNPIGEGKKHEKCFIRVYHIEEDGSLQFVYKQEFPRPILAIHAFQGRVVLGFGDELGIYELGKLQMLRKSGLRMEEHGITNIVDIKSSGFRLFVSDIRSSVQIVLYQADSNSLVPVVDDCINRHITRGFVLDHDTIIVGDKFGTVTVLRNPVSNLEIEQGHGRKGNNLKDNIKFDMLMSFYVGDVIVGLFRGQIGVGGEDAVVYGGINGTIGVFSAVKTMKEVNFFRELEKLMRNVLQGMKIDEFGQHDSIDESRQLMLAERDILKFRSYYVPKRACIDGDVVEDYFSLDDKTKGEIAKALERTVPEIEAKIVEMMGRVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGV5</accession>
    <name>A0A1E3NGV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aminomethyltransferase</fullName>
            <ecNumber evidence="4">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_35996</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GcvT family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016474.1">
        <property type="nucleotide sequence ID" value="XM_019162977.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NGV5"/>
    <dbReference type="GeneID" id="30179664"/>
    <dbReference type="OrthoDB" id="431497at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03272">
        <property type="entry name" value="ABC_SMC3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000001">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000370">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000424">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041741">
        <property type="entry name" value="SMC3_ABC_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SMC hinge" evidence="16">
        <location>
            <begin position="856"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1395"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="593"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="772"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1056"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1186"/>
            <end position="1351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1554" mass="176002" checksum="96FC08570552DD4D" modified="2017-01-18" version="1">MVPFAGYSMPVLYKGQTHIQSHNWTRTHCGVFDVSHMLQHRISGPNTTRFLQQICPTDFSVLKPFQSHLSVLLNKTGGVVDDTIVTKHGDDSYYVVTNAGCRDKDTAFLKEELAKFDQAQQIKHENFEGTLLAIQGPKAQQVVAKFTNTPLDDLYFGNSRFIDLQGFGTDAKFHIARGGYTGEDGFEISIPDSKVGVDFFNALLEYDEIVKPIGLAARDSLRLEAGMCLYGHELNDDVSPIEASLNWLVSKSRRSPEGATFNGADRIISQINNPKSVSFKRVGLVSKGPSPRHGNKIFSAEEPSKQIGTVCSGSPSPSCGGNVAQVFLDKPYNKTGTKRVIIRGFKTYKNETVIEDFSPHYNVVIGRNGSGKSNFFAAIRFVLSDAYNNMSREERQSLIHESSVNIMSAFVEIVFDNTDRRIPVAKDEVSIRRTIGMRKDDYTLDYKSATKADIMNLLEASGFSKSNPYYIVPQGRVMALTNAKDSERLELLRDVAGAKVFENKLKDSLKEMAITGQKQDQIKEMLTDIEKRLDELDIEREDLKSFEKLMSKKKMLEFNIYDREMKALSDQIEAIDDNYSTSAHETEALITELEERETQISQLSLDLNNLSSKKKLLEIDLEINHRENQEVLQQISNMNIQLQDAQISLDTLHSGSSSSERLRALKVEIEEKQQQLEATRKTLKLQRESEVELKKKINDLRQKQMSIISKKNQTSRFSSKRERDRYLKKEIVSLKDSQLRKEEGLKELETALSNRKMILRNAIDEKLKIEENTNLNDELAKIRGQIAELNSEARNLVDDRSLLWRKESKLNNIILAKEENLATIEQSLRGGVDRMTLNALETVKAIAKRLGPTIESGVYGYLGELIDVSEKYKTAVDVVGGNSLFHIVVNNDQTASILIEELKKERNCRATFIPLNRLKNEEFKFPAGSESVPLIKKIAYDEFLAPAVKQIFGRTVVCISIQKGYELSDIYSVNAVTLDGDRCDNKGVLTGGFRDTKVSRVDYLKSLRSCKAEIYSLKEELKTAKEEINSKDFVITANYQKTSTLKDSLNVKLSEKSELAARLSKVNNSIAKYRQDISTIEQKLSAIQTSVQVIEQKINNYNDELETSFSQDSFSMEDEDLLREFYEELPLLEQNLQSLTEEMNPVELKISSLSTTIADNLVPRYNQLNSELESMDSMEKDTDYILADLKSRLQKLQKRKAELKDIENQLTIDVSDIKESIESTNTQIEKLNSAQESFVKQFEDYGKSTDKNLVKKVRLRNRKQELERNIGELGVLPEETLTIYAGQSATSLSKKLTQINEQLKAYNHVNKKAMEQFVKFAKQRDSLKERISELSEAKSSIEHLIKVLQNRKNEAIIRTFREVSVGFSKIFEKLVPAGKGRLIIQKKNLEQNANTISLSQYPDTNEERNGNSANEIEDADSNDNEKSIESFIGISISVSFNSKKNEQLRLEQLSGGQKSLCALALILAIQSCDPAPFYLFDEVDSNLDAQYRTAVANLIHNLSRNAQFICTTFRPEMLKVSDKFYGVMFNNKVSTVSDIDAERALDFVEDQHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGV7</accession>
    <name>A0A1E3NGV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein KTI12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the KTI12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016462.1">
        <property type="nucleotide sequence ID" value="XM_019164680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGV7"/>
    <dbReference type="GeneID" id="30181367"/>
    <dbReference type="OrthoDB" id="9972657at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="33021" checksum="7D3BD39F9DF41295" modified="2017-01-18" version="1">MPLVLFAGFPSSGKSTWAQRLAEQLQSRISTLAPHEEGANMKVILHSDDSLGIRREMYRESATEKAARAAQMSAVKRDISKNNIVILDSMSYNKGFRYQLFCEAKSAATSNCLVQVICPTAVCFELNAAREDRQHAWQDDLLAQMIARFEEPDGAKRWDSPLISVAHDEAELPFDRIWEAVALKKAPKPNHATVLKPAVAADYLQQLDRLTNQVVSDLVAYQQIDAFGTLRIKNTDASDCFVELPSLHTSTATLQRIRRNFLNLNRVRQLDPDRIIPLFTEYLSNHLSSDGAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGW0</accession>
    <name>A0A1E3NGW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein HRI1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_173616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015696.1">
        <property type="nucleotide sequence ID" value="XM_019161566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGW0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGW0"/>
    <dbReference type="GeneID" id="30178253"/>
    <dbReference type="OrthoDB" id="4045395at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.310">
        <property type="entry name" value="Protein HRI1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.320">
        <property type="entry name" value="Protein HRI1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031818">
        <property type="entry name" value="Hri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043047">
        <property type="entry name" value="Hri1_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16815">
        <property type="entry name" value="HRI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29752" checksum="69875DCFA0E6720B" modified="2017-01-18" version="1">MSLSKRHSLFWTESEDKESEPTSTLVVSSPNGLYVDIRPFTQESEESTEENGGFDWFFAGFEIPFADSSKVEFNHEFFDSAYINHYYKTGFSGKGFTIGTDLGDFSDSKNPDEVKRGIRVETGVLCNPKTGNVEPYVEKWVTCNSEHTPELKFIGDYKSKLRCIVLDTKDGGITDNELKTDAVIGRFIVLDKWVQGVIWDKSVTNKTDSLGVVRFCDETHPLITYGGQIDKFPKLLDLKRGLKVDDSVIVEGVKWIVKEVNSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH03</accession>
    <name>A0A1E3NH03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pre-rRNA-processing protein RIX1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_25035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016524.1">
        <property type="nucleotide sequence ID" value="XM_019162376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH03"/>
    <dbReference type="STRING" id="763406.A0A1E3NH03"/>
    <dbReference type="GeneID" id="30179063"/>
    <dbReference type="OrthoDB" id="20900at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012583">
        <property type="entry name" value="RIX1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08167">
        <property type="entry name" value="RIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein RIX1 N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="721" mass="79922" checksum="C152DF73871DA4AA" modified="2017-01-18" version="1" fragment="single">LLLKLLANPSIINSASAVNQETLLTRISTLLKSNHSFIRWKATKLITISLTHPVLLLSNHTTNVISALVKILDSKCFIANYENPGQRELVTLRSTADCLGFVLDQIRGKPTLTREVLTPKLPGIIGSLIETIVLIPESAIPVLAKLLITNTTTFRPFGSKLETALKNLLNNGDNWSKIGSDLRSMVLKSLALVSFILSREKQASSWKENVDAVILELKSVISIYEAFLELSNDTEYIVKFDSLPKLPENMANSKLIFGSLSIDINETPIAIFKVSQRIEILSDLLLAYIEITTPSAITVPFGNYLSIAEILGGLNLNYTPVKRDIRDVATRELIEQSVSEAKLSGIKILQAFAEKFPGELYPHMYDILALLDAAIPVQTYRGKIKVDKKAVYEQESLINRILQTASSYLSLTERFNDMTVLGRLLEAALILKETREPTIPTENQNTAKDNVSNGSRKKVSKNKSAISLSDILSHQQLFEHPPSATTLHVLRRFFNVLLTKCELSPGKLSLVLRFVIIDAVANVNAAKMGKHGVERKQVLQLLENALLFPGKANNSISIIPMVANLIGSQSPIFSLLTNPRFPLLQKKISGSSFENNEAAHTEEEEMEEAAKFEKGEFPIPGSVSEKRAAEGDSLVDTRKTKKQHTEDEDQVFSNEKEATDILKRLKDELKEESNHPQSSLADDVAEQTEMAADDDVALSERDESEEDIGSDFEIPEINVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH80</accession>
    <name>A0A1E3NH80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lipoyl(octanoyl) transferase</fullName>
            <ecNumber evidence="3">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_22121</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016597.1">
        <property type="nucleotide sequence ID" value="XM_019162236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH80"/>
    <dbReference type="STRING" id="763406.A0A1E3NH80"/>
    <dbReference type="GeneID" id="30178923"/>
    <dbReference type="OrthoDB" id="19908at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF7">
        <property type="entry name" value="LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="54"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="8">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="173" mass="19258" checksum="725DF17D990D8783" modified="2017-01-18" version="1" fragment="single">PTILTFEFSSVYTGGKRERDSKKATNIPFLSGNKNDVQEVTMTSAGVPYIQTDRGGQVTYHGPGQLVAYFIWDLKLWRKLSSKCFVNYIEQCSINTISHVGVADVCRTENTGVWVSKGKGTEEKISSIGLNIKRNVTSHGLSINLSTNLKYLNNPEFVMCGLKGYHQTSIEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH93</accession>
    <name>A0A1E3NH93_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016041.1">
        <property type="nucleotide sequence ID" value="XM_019164565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH93"/>
    <dbReference type="GeneID" id="30181252"/>
    <dbReference type="OrthoDB" id="3997714at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="251" mass="27084" checksum="FDEA9932E1958F2E" modified="2017-01-18" version="1">MSETPSSIAVNSTAATFGDDAVQDVAAGQEHGHEQMEQMEQDGGAPSPAPAPAPRGTHGSAGTLVAELLARVPADCDAELAQDPNLAAFVEFHKLVKDFQPAESSGKEAARVFLRPIPKYNWDTLSLAFEHVYKLYTAEINDILADFDRLDIKRSIWQESAFRMDGERASRRFRAIETWISDSDLYVNVMRKDLSSSVQIIKHTLDKLNNPDPGAGAAAAPGPDFDLDHDLDPAISDSTPGPAKTSTPTPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHK3</accession>
    <name>A0A1E3NHK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016743.1">
        <property type="nucleotide sequence ID" value="XM_019164396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHK3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHK3"/>
    <dbReference type="GeneID" id="30181083"/>
    <dbReference type="OrthoDB" id="5586015at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013962">
        <property type="entry name" value="DASH_Dam1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08653">
        <property type="entry name" value="DASH_Dam1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="400" mass="44871" checksum="27D7A4DDD5F69DE0" modified="2017-01-18" version="1">MGSVNGTPHKEKKMSANKDTIPTTPKRTHSTRYPLSSPYSGKSPLPMPILLPSQYSIGKSSDRPSSGRFADSGLTTGNRRHYGPSDSKRPVGGVATDDDIEELEKRIELFDKSVTREQPEIPPVGSSLEDMPFFDKHRSSEYVSALRGIHKQTGSLKKINDTVVDLNESLGAYLFGLFQNAWCVNLNENVTPATLVKLEEVKRLKGQVAELEKQVELVKRNAIVREQRRKSTMPPPSLSRFQNSSTRKPDSRVARPARTVEKGNVSKPHPRGRTFLRAGSRNATFMARATTNRSAKSTQLQGKTSYGKGLDLSYATKIQNVPSLEDNSMSESSENDTSEVKTLTNIMRLRETNLPKRDDFRSRVKSSNNGPQRRTKEASEFPRQQKGSESWEVRHRKPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHM3</accession>
    <name>A0A1E3NHM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16901</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016709.1">
        <property type="nucleotide sequence ID" value="XM_019161389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHM3"/>
    <dbReference type="STRING" id="763406.A0A1E3NHM3"/>
    <dbReference type="GeneID" id="30178076"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09250">
        <property type="entry name" value="AP-1_Mu1_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="198"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="482" mass="54975" checksum="CB9AC6FBBEDD86CA" modified="2017-01-18" version="1">MASIVYLLDAKARPLIHRDYKGDVPTSLVDSFPLLLIRNSSSSSNMTTNLSESECPPVIYHNGHSYSFLIHNDIYVLAISKSDINVFNVLVYLNSLVEVLTHHFKELNEISIKDNNSIIYELLDEMMDFGIPQVTDDNILKEYITQQSFTFEKLMSSVSASATSTHKKIKQGKQDRRAPPTTLTNQISWRPQGIMYKKNEAYLDVIESIDMLINSRGQILTSEIYGNIRLKSYLSGMPELQLGLNDKFINSGLNSIRGLTASSQTAAAPTTVDSKKSVEVEDVKFHQCVKLSKFEADKVISFVPPDGVCDLITYRVHSDVLKPLFLIDYKFKNHSNTRLEIMVKIKANFKNKVSANKVEVQIPVPADIDSPKFHYQKGKLKYMPSDNYIRWKFHKIEGGKEYVMVAELMLLSIINEQELMNFRKVPINVKFEMQGFVTSGLQVRYLKINEPKLNYPSYPYVRYITKSGDHYDIRSGRYGTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHQ7</accession>
    <name>A0A1E3NHQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_59333</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">MFS antiporter that does not display functional linkage as drug transporter and performs functions that significantly affect biofilm development and virulence. No substrate for transport has been identified yet, but plays an important role in the growth in the host.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016764.1">
        <property type="nucleotide sequence ID" value="XM_019163357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHQ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHQ7"/>
    <dbReference type="GeneID" id="30180044"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001765">
        <property type="term" value="P:membrane raft assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000172">
        <property type="entry name" value="MFS multidrug resistance transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF51">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="76"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="537" mass="59118" checksum="003B586B74FBBA05" modified="2017-01-18" version="1">MSSHTGSDDDESDGTSYVDGVRSLNNNLAIVNGRDDDDDNNANDDNDCKEKLELLRVTSTKENEYTILSKRERVSLALLLALVGMCSAMTMPIYWTALPEMAVAFHTTEEHINFTVTSYLCFQAVAPVFVSSLSDIFGRRPVILGCIIGGIATNVGLAVSRTYWLIVFLRCILATFLAPLVSITSAAVGDFTTRRNRGGLTGLTSGFTLIGQGVAPFLGAVMDTAWKWPAIFWFSAALEGLILAIVFVALPETHRGFVGDLSVRPKFWIHRSPILAYFGNRIVGYNQSLLDKRLHKYEPWKPLKLVTRISVFNILLPSSLLFSVWTISQTSLSVHLSSDYHYSTLHVGLCFFAPGSATIVGTLVSGRLLDIIYKKRKAKYDTKYISDIEKTSITPIPPFNILLVRLYCIPFAACIVCMASVVFGWCLEYRIALAVILVMAFLITFFCMFPLNITTTVLVDMFPEIAGGATALNNLFRCGMSAIFVSCLTKMEDNMTLGGTYTFMAGIAAVSTGLVLVLMKNSESILIKAREKKMRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI69</accession>
    <name>A0A1E3NI69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_12292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016958.1">
        <property type="nucleotide sequence ID" value="XM_019159909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI69"/>
    <dbReference type="GeneID" id="30176596"/>
    <dbReference type="OrthoDB" id="10431499at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="47350" checksum="471C12AD26A0FADF" modified="2017-01-18" version="1">MSNRLHIDDDWIDNIELHSSSIKDTINAALTADEDKATTDINYIKNLIDRLIPQNVNPEEKAILLLFVLQGYSEQNDTQLVAAEIRGFFTTLCLTLLHDLNFTKIYRNKILQALFNEMLLSYLRVCNELKTARLFEAFLKFKQQFLDNNLQFFQVHVVKQLFYHGSYDKINYILAKYNIDDFDGMLDRELIENGLFSYYHQLSHSATVSKSYERAYQIIHVTMDLYLFVNIPEFQLVLSEYIFLSLMLNKGAKTTDVTIYKYKTKLFPRLLKVYQSYQAYDLESFVFNYLLYIHHLHHNEQKLLSQLFNFASLDKLSHQLIDNKLGQIAGKFNKEFIFEQQTMINTLISEINKGLLVHNFQLSEYRYKTRSTAQNADHKLLLQDCKILSSFTEGLQHAQSLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIK6</accession>
    <name>A0A1E3NIK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="4">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_147148</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017019.1">
        <property type="nucleotide sequence ID" value="XM_019160495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIK6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIK6"/>
    <dbReference type="GeneID" id="30177182"/>
    <dbReference type="OrthoDB" id="15235at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="368"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="539"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="692" mass="77108" checksum="02E4EBC6A2246311" modified="2017-01-18" version="1">MCGIFGYANYNVGRSRGQIINTLVDGLTKLEYRGYDSAGIGIDGENDWSEDSPVRAIQLYKQVGKVSNLKEEIISHHPDVSETFYSHVGIAHTRWATHGGVTQVNCHPQSSDDTNQFVVVHNGIITNYRELKMFLKAEGESFKSDTDTEIIAKLFNYYYKKDPDLEFYQLTKDVLYQLQGSYGLMVKSVHYPNEIIATRKGSPLLIGVKTEQKLKVDFVDVEFINDSEQSTEANGSYLPSKEYTNSSLRKSESRAILPDHNMPIEFFVSSDASSVIEHTKKVLFLEDDDIAHINHGELHIHRARQSEGMSTTRPVQTLELQLAEIMRGGYDHFMMKEISEQPDSTFNTMRGRLDFKNKNIILGGIKQYLTSIRRSRRFQMIACGTSYHSCLAVRSLFEELTGVPVSVDLASDFLDRSPPIFRDDTCIFVSQSGETADSMQVLKYCRAAGCLNVGVVNSVGSSISRETDCGVHINAGPEISVASTKAYSSQFIALVMLALQISNNVIDNKQRHEEIINGLEEIPRLISQVLEKKEEIKEISLKYLHGKDSMLVVGRGYQYASSLEGALKIKEISYIHAEGVQAGELKHGVLALVDSNMPVVAFATQDSFSPKVNSAIQQITARDGRPIVVMTEGDNLIDRSKTSACIEVPKTVDCLQGLVNIIPLQLMSYYLSIAAGHNPDRPRNLAKSVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIM0</accession>
    <name>A0A1E3NIM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_34291</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017092.1">
        <property type="nucleotide sequence ID" value="XM_019162875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIM0"/>
    <dbReference type="STRING" id="763406.A0A1E3NIM0"/>
    <dbReference type="GeneID" id="30179562"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133379" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="19"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="204" mass="23214" checksum="176D47E5E5BCF620" modified="2017-01-18" version="1">MCCWLGLAQATALTFTLAPNEKQCFYQFNNKPDSNIGYYFAVQAGGSFDINYTIKGPDQVLIVVEEKQRQGDYAFVAAKTGEYEFCFDNEMSTFAEKVVDFEIKVEDEDEASQMKANLPNAPTENTAVAGMQLSVNKIERTLNLLEHSLNYYKTRNNRNQSTVKSTENRIFWFSIFDLGLMMVMGAFQVAVVKFFFQGSRKQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIU2</accession>
    <name>A0A1E3NIU2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017168.1">
        <property type="nucleotide sequence ID" value="XM_019164507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NIU2"/>
    <dbReference type="GeneID" id="30181194"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000131">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF123">
        <property type="entry name" value="TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="857" mass="97909" checksum="F2557907609C8E29" modified="2017-01-18" version="1">MTKPTEGIKKWASPKDFPSDLKLSGRIINCMTQLPNEITKTADGDWKISHLRGSSALYASNKFLVDETEWETHLVAWTGEIYTQDVKIATKVKDMHSACKETPEADPLYLTENDKTTVTKMIREKNENDNIHPVWLLRRDQDRWRKYAENILWPVFHYMMNEPNDGREEIDWWHDYVKFNEAYAVKIKEIAKPGDVIWIHDYYLLLLPQILRMELPDVYICHFLHVPFPSSEYFRCLPKRKILLDGMLGANQLGFQSYSFARHFISCCTRVNGYETTPNQVTVHSFTSNVVTLPLGIDVESLQHDAFTPKIEGKIKTLKELNQNRKIIIGRDRLDSVRGVVEKLQAFELFLDIYPEWIGKVVLIQVSFKPYYHSSKLEKKVNELISNINRKYSSLDYSPVLHYNMRINKDEYLALLRVADLCLITSIRDGMNTTALEYIVCQKLKCSPLILSEFSGTTTVLQKSIQVNPWDSVGVANAINESLLLSDGKKLSIEHNLYEAVCNNTNQDWSSKVISTLLNFVSTQTATHGTPYLNRPLLLKNYQEAKRRMFLFDYDGTLTPIVGDPNAAIPSARLKSFLEGLTQDPKNEIWIISGRDQAFLEKWIGSHYPRIGLSAEHGCFMKQAGVEKWVNLTESLDMSWQTVVGEVFSKYTEKTPGTFIERKKVALTWHYRKADPILGLYQAEKCREELVRRLEGKYDVEVMSGKANLEVRPKFVNKGEIVRRLVLCTNPIDQTLKSVEELPDFVLCLGDDTTDEDMFKALNTIERQWATDKIPVNDFGTHGIYPVTVGPANKSTVAKAYLADPNQVLDTIGLLLGKVSLFETGGTVELDDRGHLKNSESAINSQANRAAYEKASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ22</accession>
    <name>A0A1E3NJ22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_34882</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017255.1">
        <property type="nucleotide sequence ID" value="XM_019162912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ22"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ22"/>
    <dbReference type="GeneID" id="30179599"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090443">
        <property type="term" value="C:FAR/SIN/STRIPAK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030952">
        <property type="term" value="P:establishment or maintenance of cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990813">
        <property type="term" value="P:meiotic centromeric cohesion protection in anaphase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031030">
        <property type="term" value="P:negative regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000062">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="256"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="295"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="295"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="337"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="376"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="415"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="535"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="577"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="607" mass="69207" checksum="CDFD75749096C2CD" modified="2017-01-18" version="1">MSQSQVEELYPIALLMDELRHDDVASRVQAMKRLDTIAIALGPERTRNELIPFLEDVIPEDEDEVIAIAAEELGKFVPFVGGSQYATILIPVLEKVSACEEPIVREKAVESLNQISSSLSSQQITNDFIPLISRLAKERWFSLHIAATGLFKSVILKVSKSERLELMKLYSDLIHDDSPMVRKAAGKELPELINILCQQFNTPDNLEDLNWNLITNMYEALVNDNQDSVKFLSVDVLISILQFLKKINETSQVDDLFTSLITLINDPSWRVRYMVADRFDSLTNNFNDEKYTLKLVPNIISLMKDNEAEVRKAISKQLPGFAKLVNAADSSIVLKEIVPVVNQLSMDESETVRSALASEITGLAPILGKDATIEYLLPIFVEMLKDEFSDVRLNIISKLQIVNEVIGIQLLSESLMPAITALAHDKLWRVRLAIIQQIPLLAEQLGISFFDETLGQLCMEWLWDPVYSIREAAVENLRKLTEFFGEDWSKDEIINKILEKRQTKEFDNFICRITCLFTFDNLISVVSPETVKEYLYPFVNEMADDKVPNIRFNAAKSLYRVAQSLVKNYKDIVLEEIKPTLERLTDDEDEDVRYFAQDNLSKLEELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ59</accession>
    <name>A0A1E3NJ59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_21426</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017219.1">
        <property type="nucleotide sequence ID" value="XM_019162200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ59"/>
    <dbReference type="GeneID" id="30178887"/>
    <dbReference type="OrthoDB" id="1588579at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133385" description="Sugar phosphate transporter domain-containing protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="8">
        <location>
            <begin position="7"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="8">
        <location>
            <begin position="311"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="437" mass="49213" checksum="46D43DA971781BCC" modified="2017-01-18" version="1" fragment="single">LPMVNLKLISLCFIWYLTSVVSSSTTKEILKDFKYPVTLTEIQFVMSGIFCMVLLISVKYAKHMLNVNCFKNFPDGTFPPLLTDTHYSVRKNFLYPSSLILKTTVPMGMFQFIGQIASHNSTSIIPVSLVHTIKALSPLTTVLIYRVVFKKKFSLKTYLTLIPLVTGVMLCCTRNDNLNISDSLFFKGSAFAFISMLIFVSQNIFAKKILTWDDGSSTKETLKPDPHQSTNSDDEDDEDVERRAKDFLNRFNWRITRAESATSTPILPISMSPPKTPKSIFNMFGGSSAPPSPTSLPELSGFNVKGNYNLSNSSSQKLDKISVLFYCSVVGFVLTLPFYLLSEYRNMNNKLQFSLSDLNPELTYLILMYGLSHFVQSLMAFQILGMVSPVNYSIANILKRIIIISCSILIEGITLSMQQWVGLTLTFVGLYCYDKWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJF1</accession>
    <name>A0A1E3NJF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component SEC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_16186</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017385.1">
        <property type="nucleotide sequence ID" value="XM_019161118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJF1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJF1"/>
    <dbReference type="GeneID" id="30177805"/>
    <dbReference type="OrthoDB" id="10267033at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="8">
        <location>
            <begin position="52"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="7">
        <location>
            <begin position="411"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="770"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="812" mass="94555" checksum="3F3425182D541B8D" modified="2017-01-18" version="1">MPVKDNEVKTNANNDDKLIEYALSSQEYIDQFIPIIKSELLNKNSLDDLVDKLETITTQKDADLQEISFHSIDDITNSVKAINKIVNTSDNISKQILVINKNLNKTGKLLLEERKKVYNLKQSKQRIEETNNKINSCLETLNLTSKILNLIKSEDFYRSLILLQSLSTNYLKEGEVFDFINKFFEALPSFKLLIIDETFNQLNRWLNLSIEKNLSEVGELLFDNFNAINEQWFEQQSQNLQLINYKVNSPVELSFRPNKYKSFNPLMKVNVDLQPLLHSILVFDELDQIDELKEYIANDMVRRIDHLFIPIKDSINKFNVFSSNESLRINLFSVCSFSIIDKIVNEKTSFKLRSLKEVNSTFNLILGKILPVLSDHIDYHDTDLGDLMELNDIVGLFYQILTNYSFSCEPIYKLLLKIFKVSTKRLVENFKTSYQRISLDDNSQPLVIDSLKEFNQVSNEVFYIFHVQNPKFPMNVPFSSIYLDTCKLLKDFIDEIYTFISKYYNSENNMIISTISRSIDEILIDIILKDLDDKIHSTYKEVVSQNLINLDFYSSSIYEIEKYLNYSNNENIMRSRNFSNLIKLNSIEEFKKTRRLAIESMFNMIDVKVHSLFDMVDFNWDSDELRSDPNTSLIDLIEFLQDSFKLNFSHLPESIKSLLLLKILDKIAKFLNDSVFESDVLTESSIKNFQLDINYVESSIVKFYDSSLNADDPTLEPLKNMFTKLKQIIELLNEGNLDSYKQDEIRLRKYNKVDPEEAVSLMNKLPTPEVEDDGTLISPVDNDVVSSMDPSQDDMRSIFGLKRTATFQFKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJH0</accession>
    <name>A0A1E3NJH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_27483</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017392.1">
        <property type="nucleotide sequence ID" value="XM_019162498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJH0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJH0"/>
    <dbReference type="GeneID" id="30179185"/>
    <dbReference type="OrthoDB" id="29661at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="174" mass="19482" checksum="2126B57836B210FC" modified="2017-01-18" version="1" fragment="single">ILSNVLGFSIVVMGISRTKIPTFRIATLFLTLFFLYDIYFVFGTDVMLSVATKVDIPAKLVVPNVVSREESKILTSMLGLGDLALPGAFVALCLRFDLYRFHEQHANCEFHHLQRFGKPYFVAALLGYTAGLMLTFKIVQIYQVGQPALLYLCPGVLASVYATALYRGEFGRLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJL9</accession>
    <name>A0A1E3NJL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_32786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017457.1">
        <property type="nucleotide sequence ID" value="XM_019162786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJL9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJL9"/>
    <dbReference type="GeneID" id="30179473"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF279">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="39"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="457" mass="50911" checksum="24592444AE80FD40" modified="2017-01-18" version="1">MAAASNYFPVANPTLPFWHSEEDSFKNLRSTPELPQETDILIIGGGYAGAAVGYWTYKEAGSTPPKVLMLEARFIGSGATGRNGGHLKPDYYREYLSFEKRFGQKDAASIGNFEHDHLAALKEAIEDLNIDCDFVLTRACNVHLTKGAVAGCLKAMQALRKNPYTKVLNDIQVQFGSNAEITSICEDSPLSLTFTAGHLWPYKMVKGFLQYCIEKGMNVQTYTTVTSIEKQKDGSYIVHTNRGSVKTKKVVLATNAYTAGLAPEFEDRIIPVKGTCSHIKVQDGDKPAPYLSNTYGICRDFTNHEYLINRPDGTVIVGGAKPYYLKDKDAWYKNVDDSTLFAGDVKGWYETFMQNHFYTWKDAKTEVDYLWTGILGYSADALPWIGESPDQSNKYIIGGFHGHGMPRIFLSAKAIAQMMLNNVPVQMTDIPVPFMLTAERLRNARNTVLKDTGFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJT4</accession>
    <name>A0A1E3NJT4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_11406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017518.1">
        <property type="nucleotide sequence ID" value="XM_019159786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJT4"/>
    <dbReference type="GeneID" id="30176473"/>
    <dbReference type="OrthoDB" id="3993780at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="289" mass="33068" checksum="2F79CCBBE1C9B911" modified="2017-01-18" version="1">MVRKTPKTELPAEHQFSLSHILSRLPTGSQDLQNVNDSINTTVAAYTNDITEQLSSLANVENELLQKLQRVNDVYDVVHNIRVENVGNPQPLEADDGTIDSKKKKSKGMKIGLNNRLQQYNKIDSGFVELLDETVVQKKRINSLLTRLKTLEKNFSRRERLFDEKSPNKKHYAKLYNYAMKEKAQQQNSPEIKTDDKKNPQENESAPATKQTQPQTGNKLDPSKTMHRSNSLAHSEISQKKLNQYLDIENELEEIQKVQSHKLADIEREKVFNPVALVSKKTATCKVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJX7</accession>
    <name>A0A1E3NJX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Inositol oxygenase</fullName>
            <ecNumber evidence="4 14">1.13.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 14">Myo-inositol oxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_16306</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>myo-inositol + O2 = D-glucuronate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="EC" id="1.13.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13 14">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the myo-inositol oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.99.1" evidence="4 14"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017533.1">
        <property type="nucleotide sequence ID" value="XM_019161157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJX7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJX7"/>
    <dbReference type="GeneID" id="30177844"/>
    <dbReference type="OrthoDB" id="5151075at2759"/>
    <dbReference type="UniPathway" id="UPA00111">
        <property type="reaction ID" value="UER00527"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050113">
        <property type="term" value="F:inositol oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007828">
        <property type="entry name" value="Inositol_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588:SF0">
        <property type="entry name" value="INOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588">
        <property type="entry name" value="MYOINOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05153">
        <property type="entry name" value="MIOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="7 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="55"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="167"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="223"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="300"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ46420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="366" mass="42869" checksum="0D63566A5B6321D8" modified="2017-01-18" version="1">MGTDELVSSSAARVSKILDTSKYQNLDKAKLLEKIDDDIQELNLLKRRIRQLEQDGEGEKVESEVSSGSPAKQGGEEDARAVETPEEENKEVAHWKDRSKSTVLALSSSRQYRQYELAFDRVKLFYKEQHEKQTVAYNIQARINFKTKVRDRMTIWEALTKLNKLLDESDPDTELSQIDHALQTAEAIRKDNKPRWFQLVGLIHDLGKLLYFFDSQGQWDVVGDTFPVGCKFSKKIIYYDYFKENVDTKNPLYGTKYGLYSPHCGLDNVMLSWGHDEYMYHLAKEQTTLPPEGLAMIRYHSFYPWHSEGAYKYLMNEKDKEMLECVQAFNPYDLYSKTDVRYDIEELKPYYLELIDEFFPNKIVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK69</accession>
    <name>A0A1E3NK69_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_73439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017071.1">
        <property type="nucleotide sequence ID" value="XM_019164478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK69"/>
    <dbReference type="GeneID" id="30181165"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="122" mass="14403" checksum="5C9AA833185F3046" modified="2017-01-18" version="1">MVKSKKDRDNHHADDHKHEVEVKRLMKSYVKVGVSLRKIPLAFMTVKTRESTEFIHSFEYVMQYSYHNMFAKSERSSKINDRLYGLKASLKSCNETLILYAKLYSLSQQQFYTPTSYAFYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK91</accession>
    <name>A0A1E3NK91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_16419</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 15">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Cytoplasmic vesicle</location>
            <location evidence="1 15">COPII-coated vesicle membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 15">Endoplasmic reticulum membrane</location>
            <topology evidence="2 15">Peripheral membrane protein</topology>
            <orientation evidence="2 15">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
            <orientation evidence="15">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017670.1">
        <property type="nucleotide sequence ID" value="XM_019161200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK91"/>
    <dbReference type="STRING" id="763406.A0A1E3NK91"/>
    <dbReference type="GeneID" id="30177887"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000041">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000043">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 15" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0813">Transport</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="17">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="18">
        <location>
            <begin position="119"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="20">
        <location>
            <begin position="392"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="19">
        <location>
            <begin position="517"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="630"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ46557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="772" mass="86826" checksum="3762E1D34F68ED09" modified="2017-01-18" version="1">MDVLQSQFETYEDKTGLKFPFNVFPRTKEQERKCSIPISCLYQPLRPKEDPVIVENYPMACFNCKSIINSLCMADSRGWSCSICGNRNQFPNEINQLPIEMNPGLLDIEYIIPQQGILKPTIFVYIVDLAIDEEELASLLENLLISIDLLPVNSMISLITYSKHVNVHEIGYQGSNYSYTFNGAKDYSREEISQKLGILRKNVDGMKNATTSQIANRFFQQTAICEFSLASLIESLQKDAFKVPQFHRKERATGCAINVAFHMLGTLYPKIGARIMLFTGGGCTVGPGSIVSTSLKDPIRSHHDLSKDAKTIKKFKANAKFYNDIAEKASINGHTFDIFIGCYDQIGLSEMECLVDKTGGVIVQSDSFTSAIFKQSLNKFLSPNEYGESQFGLNATLEVKCKNVKVRGLIGHATALHLNKGKTLDGAHNKISFHTEEKGKKIGLGGTNCWKLGSVSTHSSYAVYFEMVDHPIGDYSVIQFIASYQHPDGTQHIHVTTSQRFMNQGSDSMDSIISNFDQEAATAIIARQAIDTVANNSSVNALTFVNKILIDFLSVFAKYRVNDTRSVVIPPNVNLLPQFIYHLRRSNFIQIFNSSPDETSFYRHCFLTEDCLNTLIMIQPTLTAYELEKDPEPVLLDSTSLKPNRILFLDTFFHILIFHGSLISDWRRQGYQDQPDYEYFKDFLELPRQEAADILVDRFPLPRFIDTEEGGSQARFLMSKLNPTTSYNSSDSLEQLATFGPQDTGDGAVIMTDDISLQTFMQYVYEAVVKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKA7</accession>
    <name>A0A1E3NKA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_21803</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017687.1">
        <property type="nucleotide sequence ID" value="XM_019162222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKA7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKA7"/>
    <dbReference type="GeneID" id="30178909"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="78" mass="8941" checksum="8ABC45EA250236AA" modified="2017-01-18" version="1" fragment="single">ERKNSAGDIAGSDIPRNYKCSNCGISFKRSSDLKRHEKIHLKVPPNICPLCHKGFARKDALKRHVDTLTCKRNRQRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKB7</accession>
    <name>A0A1E3NKB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017705.1">
        <property type="nucleotide sequence ID" value="XM_019164263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKB7"/>
    <dbReference type="GeneID" id="30180950"/>
    <dbReference type="OrthoDB" id="3995742at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="457"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="481" mass="52695" checksum="1D6CBF68DCCB2341" modified="2017-01-18" version="1">MSDSELLALDSQFNVRPVDIQKSYGFAAGPRLSSSSLSNCDKDSLDSGSFKNLSPNAILNEYPTKPIITKNSICFNYKHADLRNSDLNDKFYLIMLSSKSYSLSSLDYYINKISSKGDTIVICCSLSTSILGNDKNEMLDEFINSFTNLILSHLENSSSLVHPINITLEFFKSGAFFAEVFNLYQPSLIIVGTNNAKTKSTSMTTANRKLLSVVYVGNDQILNPPASDYSNGNAGQSVKSSNAQVTFKVPFKSKDEGSNDAVKQKQTPKIVVDPPRISHTVSHSSIDSNSSDSNKLSKMKTNDTQTSSLFDNLNDTPLLDLCKTSSNSTSTSYDFLRTDSNSCDTSRRRSMLDVLNSDTVLSKSSSNDDSYQCAIDDSDDASPECNRQNQLGRRTSRSSSGSFENPKPLVGHEKQKQELFEKYQRRLSAVKVGPQASKSGATPTTSNNVNPSATNDKAGNSKKPPSKGSESKSIFKKWFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKC5</accession>
    <name>A0A1E3NKC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016896.1">
        <property type="nucleotide sequence ID" value="XM_019161446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKC5"/>
    <dbReference type="GeneID" id="30178133"/>
    <dbReference type="OrthoDB" id="3996652at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="133" mass="14825" checksum="B7117264568FFD12" modified="2017-01-18" version="1">MVSPIPRPQPPQPRNTKTGATKPVSFSPQSETQTQAESVTQKQPQAELPPQGGSAKLSAKPSLYAKYRALSLKVKLYIWITTAGAAWLADSLSDRIFEQNMIDAEANRRVEMEIRKMRELEVLERNSASSNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKG4</accession>
    <name>A0A1E3NKG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016936.1">
        <property type="nucleotide sequence ID" value="XM_019161459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKG4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKG4"/>
    <dbReference type="GeneID" id="30178146"/>
    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="508" mass="57249" checksum="B33D58D765B6F8EA" modified="2017-01-18" version="1">MNTKVNKKVKLKPATVSSLNYPLSSNSNRKSESDLDVLLMIIADVERLFPEHPEMFIESKEDKLQMIEILYRYAKWINDSREAAGKKKLGYVQGMHELCGVIYAVLKVELVDNKKKEYTTENSTKSNIVDGGEDITDLSRATSKTKTSSSEQADSLVSGKINEKLEMQIKEFLDKNFFAHDVFAMFKQLMSPIIDKYFTASGIVRESIVFDLKLHHLDPGDAKHPGLAASFKESQIESQLWLTRWFRMILTREVGLAYAVRIWDGLIAYACAGAMADTATNGHDVSALLPYVIILLILRVRSALLKSLVPCLKKAFDEENEIDDDTEALSLLLHYPSHNKNSSFSRSTSADSDSDSEKERQPIMMYRKTRDKKNNLNTHRSQLTKALQIPKLPSAVDLFSDAAHICGLTDSELTQIGPSLIEKYSGGDIYEALAAYDKSKPAAPFIDGVLKHTKAWRVSTATNKETKLKLEKKLTPTILDSNRTRLELRLQERVQNRLRDRSHGSLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKI4</accession>
    <name>A0A1E3NKI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_16475</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017743.1">
        <property type="nucleotide sequence ID" value="XM_019161224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKI4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKI4"/>
    <dbReference type="GeneID" id="30177911"/>
    <dbReference type="OrthoDB" id="18884at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027512">
        <property type="entry name" value="EIF3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054711">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22591">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="355"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="625"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="681"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="819"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="893" mass="103342" checksum="DEA0A186E22279C5" modified="2017-01-18" version="1">MSYQAGRPENVLRRAEDLININQPDVALQNLYTFITAKRTKNIEPTNTQLLEIFKLFISLAVNNRLSKDIKDALYQYKKMAQNSSGTSGFENLEQLTKYFITIANSKLDEARSKANVAAEAAIEKSVASAAAAQKVIGSIDDDEEGEDDETFTFNVSPEDILLSSVTTDDTTDRSNKEFFLPWLRFAWESYRTVLELLRGNNKLESSYCQVTLQAFQFCVRYERKSEFKRLCEILKTHLLSFTKEYDDSVNLSNPDTLQRFLDTRFQQLNTAVELELWKESFKSIEEVHSLMKQSKRTPKPIAMINYYNNLAKIFQVSNNYVYATAARQKLFQLLIQSPVITDAELKENASIHLVTSLSIPLLEYFDHSFSAQSDLVDAEFFKRKNNKLSSLLNLNEIPTRESLLGSSTLKEVLKYADSEIVQLYTLLEKSFNPLSFHSSVNSILNTIVKTDKYAPYVEPLKKVFISKIISKVSTLYESIKFDFLYKICTFENAGFNYDEYTFEDIVTNGQYTGLINADIEFDEEAGVINFTDKLYVTPTSKVNSRLYDLRRALSSAIGLINVQQSADVEKERQAKIIKSAKENFEDEKKTILQTAALIKERAELLLETKKNEKLEAERERAEEALRKKRAEEERIQQENEKRENDKREKTQMSILMENKKTLVNEINSKGVIHIKFEDVKDMSEDDIRALRAEKLDAESKKLEEQTDAIVKRADYIERAQRQYEIPILEKEIEKDVSKQREEYEAMKAKNLEQSKKSHEEALHVKERLARFVPTYEEYTKKLDALVSDTVEQRKQKAAEAFEAEKKARIQAFIDAKKREFLEEARRQAEEERAEIEAAKEAERVRAQAAKEEERRRYAADPDRAEYESLMAKSKLSMSEKLKLRRLALKYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKL2</accession>
    <name>A0A1E3NKL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_34416</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017211.1">
        <property type="nucleotide sequence ID" value="XM_019162884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKL2"/>
    <dbReference type="STRING" id="763406.A0A1E3NKL2"/>
    <dbReference type="GeneID" id="30179571"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="149"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="153"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="519" mass="60159" checksum="65CF89E70BBFEF1E" modified="2017-01-18" version="1">MSDSEEHEGSYGYDDSDEASSLEFSDVDYGDDNSLNELDHEDTHAKVMFSFPTSTTSSGIPTHRMKYQALCIDEIQKYIEQKVKELSDLLQLDNGKCLVLLLTYGWNEHKLLDDYVNCDEQRKLLLQHGVYDKAYPKDLSCLLTKETGEEVICSICCCSPEPSEPMTFFSLFGCKHKFCTECYSHYIQTKNEGSQVVIDCPFSDPKCTLKITTLELGILKEMDSKRNRTILSKYWYNVSNQYCMTQVKRFKHCPYPDCESVVEFLGFDSDNIASIEEQISFLLIPLVRCGRKHQFCFACMEVSHAPCPCKVVEKWKQKCEDDSETLNWIQSNTKDCPKCHAIIEKNGGCNHLTCTACNYQFCWLCLGDWYMFYFDHFNNQRISHDKDREILEKFESRIRELQIKAGVSWIETVFYKECVTALLECRQILKWSYAFLFYVPKCRGKQLVEAAQWQLSDKVERLSKLFADTPAANVLACKESFLYIKSSMIVAQEKFLETCIDIFADPTTMNSFKRRLNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKV1</accession>
    <name>A0A1E3NKV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitination network signaling protein acrB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_16578</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017864.1">
        <property type="nucleotide sequence ID" value="XM_019161264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKV1"/>
    <dbReference type="GeneID" id="30177951"/>
    <dbReference type="OrthoDB" id="4158994at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="458"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="685"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="480"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="893" mass="101626" checksum="07EBEC4AEB4F52CE" modified="2017-01-18" version="1">MPSSLSTGYTVSSNENNFSSAGINRAAEALSSQNGSSSTSFESKTPLDQQKAFAQSGYPSEIDQQQKIASLPSYPLLDTFSFLCILMIFPHWFSTITLILYVFLGNPEFLDSILSFVLKHKLNRTPNSSTKSSSFSLVGRLSLISFVLYLLLDAIFMLFIFYFFPAFVPYIILLSKSLIASNLTSLKNRYILDAFAACILLLTLENTILYTIKHFEIFRGDSMLSLTSTLSSTDFLYPLYYRPTASVTKTLLFHLTFHKIRENSLLMFQMEYALQFLHSSLSLYIIMHNFNPVLRKFSLINKAYTYIESFSLQEQTEQLEPISQPSDFAAPNIALPSNFSAYNNAQKPKFQNIFQIPKTISGSNLPTLDSDRIIVDDSSKKESSFSKDEDISDDDNSTDTDTNDGSEPSKNKIPMWFPEDHQVVNMNDVSSPSFVVAQNFENFRKMIWSSSAHLLTSSSVSEMGPSPGTSGSTFIPTPNSPNLSSTLASSSNSNSNSMKYKSSSFHLSHLVNSQPKRVVSKSKSFLPSEKKAKMSQLKYQQRLWLFLNAARTMFSRQDYYSGDYYSQNAVVTTGYGMDDYARNANSSSQCFIWFTGETTLAFELHNISLEQLLIKVNGIIWEHVSSCAFFGRELVIINGLSPLSQYDIDFVKITLNGELIHLTTTTVSTVFQNKTVTESNVSSPLATLQRSVVTTQEAIEREKARLKKMKTDWRKKSSQIKSEIENLNNRSNFSDESRNYKKLDSLRQIVAKSDIEVTSLSKKSEETRLLQTEIEEKYLDAKRLYETELRSFNKFENDNKNSIMALEKGIKSLLSEKNQLLVKKEKVISKKLRIHHEVELLTNELKSLKKTEIALRVERRKFRSIQRDEKYKLLINDIKKIERQLRGKTFNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL22</accession>
    <name>A0A1E3NL22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor CWC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72855</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017934.1">
        <property type="nucleotide sequence ID" value="XM_019164311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL22"/>
    <dbReference type="STRING" id="763406.A0A1E3NL22"/>
    <dbReference type="GeneID" id="30180998"/>
    <dbReference type="OrthoDB" id="3938623at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="345"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="292"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="551"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="589"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="665"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="690" mass="79195" checksum="B46CFD93D23BA6EB" modified="2017-01-18" version="1">MYEKLQELLKLKSGGVYVPPAKLKALMAEVELEQDSEEYQKLQWETLKKKINSIINRVNKGNIKIMVIELFKLNLFRGRGALCKSLISSQQLSTAYTDVYSSLVSVLNSKIPEVGNLLIRRLIVRYKRLLKREDKYGCRAVLTFLSDLVIFHVCCLDLVMEIVLQLLEEPSDFKVELVLEILNICGVEMYDNESTWFDQVKNTLRNILSEDLVDSRSQLLIQSFFDTFNKKLKEGQALDEDLDLVDEDDYVVHKFSLKDRVQGDDQLDVFQYDKDYKQHNEKYNLLRKEILGSDVESEPEEKTGDNSDFSSDNEEDEEGDDDEETEDSKRLTVQIKDLTEQELTNYQKNVYLTVMSSMSPEEATHKLLRLPSIDPSRKEYMLVDMIVKCCAQEKVYSKYYGIIGECLVVMGKAWSGAFQQLFKENYENCYKFETALLRNIGAFWGHMLASDKLGWECLSAVKLTEKDTTSSGRIFLKFVFLKLLEESGINHLVERIEEAYIEPFVIGIFPKTDAENLRFSINFFTAIGLGKLTERMRVSLENLPVEAEAIADGDDDSDSSRGRSRSRSSSYSSYSSSRSRSYSRSRSASPKDEEKENRGRGREERSRSRSPADRERSFSRSRPTSPDRRPQRGQSTIPRGPRSHRGRGYNSRGSGYRGNRGRGFGSYNNNRGSRGYSSHSGYGNGRGRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL58</accession>
    <name>A0A1E3NL58_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_127365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017411.1">
        <property type="nucleotide sequence ID" value="XM_019159965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL58"/>
    <dbReference type="GeneID" id="30176652"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="105" mass="11371" checksum="4EFC4DF8ACCBB961" modified="2017-01-18" version="1">MSRGPSWMTAAASAGVVAQQRQPSCMLLALKKESEGRFFGLILLLFLVLLCFRYMLPATFCVLFPVSDADGSQGLRSLISSIDPQCPCSLLSQCVSLFCPGCLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLM9</accession>
    <name>A0A1E3NLM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_105843</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018165.1">
        <property type="nucleotide sequence ID" value="XM_019159693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLM9"/>
    <dbReference type="GeneID" id="30176380"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="308" mass="35974" checksum="A26B89B6B3B90DA5" modified="2017-01-18" version="1">MSCVCICRRFFRHQSTCNQAPLHHGQRILVRTLRHIHAGVRLHAPQHHDAQLRDIQHQRVGQKVPRPGEVPGRRRARRGGRRGRGGRAAPRRRRPLPDRGRGHMQLHVEAKTVVRVRGRVAALAHARRVVGRGVRRRLRRGRRHGGPRRLRRVPHNSVALRLRRRVEPGHHLPLPAPPKRLLQPIPQETAADLRLHPPPHPAPRRHAYVRGRRQLQPLALLQLVWTTPHPPPLFITSLHFYYRTLGCLLLLWVFGSLGLLSGRVLWHQALRFHHYVFHYGFLFFFIFILFFLFLFFPLILLCLSSFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLT8</accession>
    <name>A0A1E3NLT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_15088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018210.1">
        <property type="nucleotide sequence ID" value="XM_019160657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLT8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLT8"/>
    <dbReference type="GeneID" id="30177344"/>
    <dbReference type="OrthoDB" id="338814at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04063">
        <property type="entry name" value="DUF383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04064">
        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="4">
        <location>
            <begin position="101"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="5">
        <location>
            <begin position="293"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="391" mass="45102" checksum="B0A4BB1EE4D1153A" modified="2017-01-18" version="1">MPTELQELVGFLHDKALNVREIALENLVQFSPGGANNQQDIFKYDNCRAIEDLINLAHEKKGKTVNEALTILVNLCDDQELRSLISKDDTFLKFLIEALPNPQNLNAELMCMLLANLAKEDNILKVFDIKVDIRDDQKEIFKSNNAMDCLMDMFVKGADRQINKYAVYDYLSYFYADLSRFKEGREYFVTEQKYDDVIPLTKLLVFTEKWDAKVRREGVASTIKNSLFDIEKHMTFLEEPINILSYILSPIALPGNKGLSDDEILELPDELQFLDEDRKIEPLRDIISVHLESLLLLCATRKGREFLRSKSVYPVVRELHKESKDDKVSELCDRLVQMLMRDEGEDKPDEEELDEIKGMLHKVEEIGDDDDIQMTDDDDDDDEAEGMVVVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLV6</accession>
    <name>A0A1E3NLV6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017671.1">
        <property type="nucleotide sequence ID" value="XM_019164249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLV6"/>
    <dbReference type="GeneID" id="30180936"/>
    <dbReference type="OrthoDB" id="10542070at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="233" mass="27089" checksum="E0C8E67DFDA909BE" modified="2017-01-18" version="1">MHLEKHETLEQVVEGLGFLETDAEKDKQMRVTLVTVQHDKIEKIVLNYSFLTPKSWNQFYTNTSVCILHSLDLNQIHRVLCREILRMQMKKLESGGYVNPLEVQGDIFILDGFVASLSIIFSQFVSHERIDYDMMNFRKVFLILDRIKKLDGLLGENVRLNESNEIWEVVDKIFIPCKKESSYSKLVEYLSDTPEDPTQKEARNVVLGCDDFVSLRHILNFFGIKGVTNIEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM30</accession>
    <name>A0A1E3NM30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_107992</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Lipid-anchor</topology>
            <topology evidence="10">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018274.1">
        <property type="nucleotide sequence ID" value="XM_019159717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM30"/>
    <dbReference type="STRING" id="763406.A0A1E3NM30"/>
    <dbReference type="GeneID" id="30176404"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000032">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF14">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027322" description="1,3-beta-glucanosyltransferase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="470" mass="53136" checksum="5F85AD70DE18C0AB" modified="2017-01-18" version="1">MIRKSIKMFHIVLFVVLTLSTLTHAIIHPIEIRGNHFYDSITNKPFFIKGVDYQPGGSSGVNEHSDPLSNPALCARDIFLFQELGINTIRVYSVNPSLNHDKCMTLLAAAGIYLVLDVNSPMVGQHLNRYEPWTTYNDKYVGHVFHVVNEFSGYNNTLAFFAGNEIVNDKTSATNSPVYIKAVIRDMKHFIKSNSARIIPVGYSAADDLSYRISLADYLECYETDPLETVDFYGVNTYQWCGEQTFITSGYNNLVRDYSTYSKPIFFSEYGCNEVLPRLFKEVSSIYSSEMTSVFSGGLVYEFAQEPNNYGLVDYDEFGNVKLLPDFFAFKEQISKVQDVPLSKKLLLQNQQNFESKNVRKQKPKRKVCELKYANLDISKGIPRSMGESIVKSFNNQEKGKFVPLTQEDMTSNFKIFSVDGTVFNKKPIVKPVPEPLADPKDSISLTSKLNPLFSLICGISIQSLLNGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMB2</accession>
    <name>A0A1E3NMB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP11-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_33633</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017616.1">
        <property type="nucleotide sequence ID" value="XM_019162833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMB2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMB2"/>
    <dbReference type="GeneID" id="30179520"/>
    <dbReference type="OrthoDB" id="16535at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="37027" checksum="AC9F0B6F0CDAFAB9" modified="2017-01-18" version="1">MRTALSAAGKLGTKNRSLFAQHAVRTASVTNVRFIQSKPRTEKQVLERYKAKLEARAKQQGLESAERLMEEMKDTIEQTKKQLNAIDPLKELDDYEQAVNLRDSLSGKKKTLDPIQPGTPTKPYKTLDSYLVLEKIRELDVQHIEFLWRAKFQGDPQSLVAVVPGDTFDVMYGHARANPTFVLPLPKADAQLDEADRAPADADTTPMEVHFVQWSFVGPKTTHCMITTLMEYKLHKEYARPHTTLSFHQELEDDKGIVLMSGHVDKDAAITPAESQILLLNLQRFYGAFRTNSDIEHQRVQLVRDFNSGSPGFSMEKCIELSQSLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMC5</accession>
    <name>A0A1E3NMC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbamoyl phosphate synthase arginine-specific small chain</fullName>
            <ecNumber evidence="3">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Arginine-specific carbamoyl phosphate synthetase, glutamine chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_15257</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018390.1">
        <property type="nucleotide sequence ID" value="XM_019160738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMC5"/>
    <dbReference type="STRING" id="763406.A0A1E3NMC5"/>
    <dbReference type="GeneID" id="30177425"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000016">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000003">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="16">
        <location>
            <begin position="30"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ47277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="415" mass="45514" checksum="CF9C2F0792BBDC44" modified="2017-01-18" version="1">MLAFNILKTRLSSQLKTQFRFASSSAKTLDPAKLTIRNGPVFNGLSFGSPKTVSGEAVFTTSLVGYPESMTDPSYRGQIICFTQPLIGNYGVPSGNARDEYTLLKYFESPNIQCVGIVVTDVALEYSHWTAVESLSAWCQREGVAAITGVDTRELVTYLREQGSSLARLSVGDDSSAEFYDPGLVNMVSKVTTKEPFHIPVKNAKHHVLVVDCGAKENILRCLVSRGAAITVVPYNYPIHKVADKFDGVFISNGPGDPIYCSETVENLKDLIKSQPDLPIMGICMGHQLLSLAAGAKTLKLKYGNRAHNIPTLDMTTGKCHITSQNHGYAVDATTLPSDYQEYFINLNDLSNEGMMHKERPIFSVQFHPEAKGGPLDSSFLFDKYFELLDKYKVSGKGKDLGDLSSKNLAGARVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMK4</accession>
    <name>A0A1E3NMK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018468.1">
        <property type="nucleotide sequence ID" value="XM_019160761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMK4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMK4"/>
    <dbReference type="GeneID" id="30177448"/>
    <dbReference type="OrthoDB" id="48651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="100"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="593"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="647"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="720"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="739"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="752" mass="81772" checksum="C7071AED95D84EBC" modified="2017-01-18" version="1">MGTKKMSLTEFMADPSLGTPSWIEDDFDITSTLNSNPFKPAAPSPFGNSGNKSNLFERSHNGFGMREARNGDFHEPGAGTSWRSSAMPRGGQSHNYKNSNIIVFDNVPPFCTRQLIKELFESRYTKLEKCHVLLDPSDPTHKKKIAFVFLYTPQDMQKVLKWNGINIERSRIIIDSASSNEFQAILQFNHELNFDEDLEDKRIEDEQKEQREQRLKRQMSPTPITATNTQPSIPKVNPFGNAKPVVVPEIPIISESTKEIAKVLDQASKPSGEPHRVQPKVNPFGNAKPVLVPEIPIISENTAEIARKLELKVDSSNKSNRANSISKSPVRAPVHPKPNPFGNAKPVDTLAKQLEIEKKLTKAAVNETTFDMTLIHGKKNKEKAVAAAAATEKEKEKESDGKSHSPGSPVKILRRKDSVGSGAVDRRRDVEESKSPSSSSSSSSSGKGYKTTSGFERRIARDLSEEEYEQEMKKRNELRSHSAGKERGRGASRRMSAGRFGNKQRHNAPPGMAAHGGHGHGVSDNHDGTPQEPYTKSVEKGKDEEGEEGAGDEKPSDGFENYVQHEHNNEENVEPANLDAVSGAGERAASDAQNKEEGKPTSSEDHNLTKASEETPVGIEERGDAREAGAESPSSPSSPSTAAMSRGRGRGHGGRGGGPRRGRGGRGGRGDYSESGWGREGYGGYVRGRGRGSSNRSRGGYNGRGGYRNLVYHKPTTEAGAKEGSKEGADANTEPRTEASAAVPATAPAKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMV1</accession>
    <name>A0A1E3NMV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_68130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017836.1">
        <property type="nucleotide sequence ID" value="XM_019163504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMV1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMV1"/>
    <dbReference type="GeneID" id="30180191"/>
    <dbReference type="OrthoDB" id="3985954at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="139" mass="15755" checksum="C40634FC6EBD44D5" modified="2017-01-18" version="1">MPGEIEKPVFRKGRSRLGCTNCKKSKVKCDEVHPSCKRCLKRGTHCSYPFQVSFEKIMGMPQDEKMKSKKYGKITTLQTLPYQPDESSSNEKNNTNGYSKPAAASEFLRSLIKKNEQDRSKVDSIDDFAKVVQVKSKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN45</accession>
    <name>A0A1E3NN45_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_23535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018637.1">
        <property type="nucleotide sequence ID" value="XM_019162311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN45"/>
    <dbReference type="STRING" id="763406.A0A1E3NN45"/>
    <dbReference type="GeneID" id="30178998"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950:SF16">
        <property type="entry name" value="ZINC TRANSPORTER ZIP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="119"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="360" mass="37669" checksum="2EE4C23CA7F079BD" modified="2017-01-18" version="1" fragment="single">VFNKLFPFGPAGNALLATTYISGPPNLILALIPSDINVSSLSLLVSFAIGGLLGDVFMHLLPETFTGQSVDLVVGDQVYVLVDNRKNVLLGIGMFVGFLLFFGIDKLMRIIEHTGNASAGGASGHSHSHSHSHASATSSGLPEEKSGELKQRGAKKDGSAATATGAATETEAVIANPNASIKTSAYLNLISDFTHNITDGLAISSSFYISKSVGCTTAIATFMHEIPHEVGDFALLIQGGFTKWQAMGSQFVTAAGAYLGTFIGIALQQSLEKDRAAASAMASSLDSSAPSGLFGTSLSYGDMTLPFTAGGFLYIAFSVIPELLETEEDTDRSAELAKFAKQLVCMFLGIGFMAFIALND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN87</accession>
    <name>A0A1E3NN87_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018680.1">
        <property type="nucleotide sequence ID" value="XM_019160849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN87"/>
    <dbReference type="GeneID" id="30177536"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="55" mass="6040" checksum="5E3C1E473EA92075" modified="2017-01-18" version="1">MVTVIESSSAVCTHVGELRDSNFQLFSCLNNYTNTQEGRHCNAGICPVLALFFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NND0</accession>
    <name>A0A1E3NND0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tafazzin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_71665</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:33731"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64743"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33732"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:33733"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the taffazin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018728.1">
        <property type="nucleotide sequence ID" value="XM_019163976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NND0"/>
    <dbReference type="STRING" id="763406.A0A1E3NND0"/>
    <dbReference type="GeneID" id="30180663"/>
    <dbReference type="OrthoDB" id="193467at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF0">
        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="13">
        <location>
            <begin position="72"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="418" mass="48050" checksum="7D6533E9BEE5CE18" modified="2017-01-18" version="1">MSFHDVLKRGDDEILSVRMERSPLWNALSHATCVAVTGFSKLILATLYNVELKGLPNLDHAFANARASNRGILTIMNHMSTCDDPFLFACLPWRYFKSWEDIRWGLAASNVCFTNSFSKKFFSLGKIFPCDRFGRGPFQSGLDACIRILSPDDTINSQYILGDEDHLTKYRSDYFDETKKDNLSPDNVLSRTALSLFSPTYVSPVLRYKTSWVHIFPEGYVCQLKPPFQNSMRYFRWGTARLILEPTVAPVIVPIFTDGFEKVKPEEVDLKTDYLTPNNVGAQITVNIGKAIDDNIISKFRSEWKELCLKYSNNENPNDLSDELMFGKEARELRSRVCAFLREQVAKLRLENGFEEEDPRFKDVNFWTRFTKSRGASDPDIQFVGLNWAIKEYQKHVKVYDDKGNVIGERESERPEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNF2</accession>
    <name>A0A1E3NNF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_32400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018745.1">
        <property type="nucleotide sequence ID" value="XM_019162764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNF2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNF2"/>
    <dbReference type="GeneID" id="30179451"/>
    <dbReference type="OrthoDB" id="5322896at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3580.10">
        <property type="entry name" value="ATP12 ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2180.10">
        <property type="entry name" value="ATP12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011419">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042272">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023335">
        <property type="entry name" value="ATP12_ortho_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013:SF10">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2/ATP12 PROTEIN, MITOCHONDRIAL PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07542">
        <property type="entry name" value="ATP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160909">
        <property type="entry name" value="ATP12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="316" mass="35519" checksum="75D7E34207D18B9A" modified="2017-01-18" version="1">MLSFTRVLTKSQPRWTRAFAVHSSAPQPASYESNKQTETNKLEKTMAKFWEKVSVGECEDGTYVVQLDQKTIKTPLGFDMKIPAAKRSLAYLLGNEWKALPSLQIRPYLVPLTSLTSRCIDLHEAQHTDDVEVKTKVGDLSTVKTLLLRYLDTDTLLVFSPLEDCDGDLRKEQEKCYRPVIRSMEQFFSRFADDGQPVSLTYLDCSKDGLVGNSQTEHTKKAVLNYLDSLNTWDLAALEKATLVGKSFLAGTAILRNADYADDFILTVEELARLVTLETVLQTARWGEVEDTHDVDKVDIKRNLASAALVAFNAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNF9</accession>
    <name>A0A1E3NNF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_15560</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018739.1">
        <property type="nucleotide sequence ID" value="XM_019160870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNF9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNF9"/>
    <dbReference type="GeneID" id="30177557"/>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000006">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="17">
        <location>
            <begin position="291"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ47626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="592" mass="66806" checksum="EE4D6A9B6BD77B76" modified="2017-01-18" version="1">MPTVAVDKQDLFDLLGKNYTTKEFDELCFQFGIELDEDTTEDVKGTSERPQLKIEIGANRYDMLCIEGIAQALNQYLGRCEPPKYTLIPAKPEISLTIKESTTQIRPYAASAILRGVKFTQRSYDSFIALQDKLHANLCRQRTLVAMGTHDLDTLKAPFTYEAWKPEDIKFAPLNQTKVMDGHQMMEFYKSDKNLAKYLPIIEDSPVYPVILDANQTVCSMPPIINSNHSKITLDTKNVFLDITGTDKTKVEIVVNQLIAMFSRYCEKPFTIEPVQIISEHNNQTRVCPDLTERSLQVESEYINSILALELSNEKICSLLSKMSLNATPSTKDAKLVDVKIPITRPDVLHACDVMEDCAIAYGYDNLVKTYPKSAATTAYPLPINKVGDILRTASAQAGWLEVMPLTLCSHNENFEFLLDNDDSEAVKLENPKTVEYEVVRTSLVPGILKTIKENKNHALPIKVFESGDIVYKVPELERGAKNQRNWCAAYAGKKSGFEYVQGLLTKIMQTMRVPWLEKDQQNTETRGFWTEGTDEKKMFFPGRGAAIYFRARPEDKAVKIGHLGVLHPEVLANFEIPFALSVVELNAEVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNG5</accession>
    <name>A0A1E3NNG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71694</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018759.1">
        <property type="nucleotide sequence ID" value="XM_019163984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNG5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNG5"/>
    <dbReference type="GeneID" id="30180671"/>
    <dbReference type="OrthoDB" id="409956at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF16">
        <property type="entry name" value="D-AMINO ACID OXIDASE (AFU_ORTHOLOGUE AFUA_5G11290)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="47"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="364" mass="41343" checksum="4ACCE263FD1614A4" modified="2017-01-18" version="1">MTRIVVVGSGVVGITNAYCLLKKDPSNEVTIVSQNFPTDFEFKKVYTSPIAGANWASFATPEDKFVQEIDTVGYYKFQDLIKNRPEAGVTARKDINYVTKEKFAKDGHAKKFPWFAYGQIGTDCGFRELDASEFDNNKFEYGFEYDGLVIRTSYYMTFLINECWRLSGASEGPKARFSLRRGSVKKLSEAFDLHASGRRADVIVNCTGLLARELEDLEPEERSKIYPVRGVVYVVKNTTGVKKVTIVEIDKEDESLYLMPRREGEMIIGGCFQKNEESRYVDPSLRERILGRCSYYLPQFNWKNLEIVREQVGFRPYREGGYRIERKGRIVHCYGTGGAGYQSSWGCAEKVLKLVSQVGSGSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNK9</accession>
    <name>A0A1E3NNK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_117889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018803.1">
        <property type="nucleotide sequence ID" value="XM_019159844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNK9"/>
    <dbReference type="GeneID" id="30176531"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="272" mass="30712" checksum="F2E849D34A7BA860" modified="2017-01-18" version="1">MDFSLSSTTTVSFDVSTKQLSHYTSATPESLLTGYSDPLDLSESPLLFSSSYVPNSPLPSASSPSVADLHYSHNISISSNSCICTGDDSCNTACDRRHYCYNRYRQDCPESSTPHRMKNKQHTENMDSHMAYTHFETSPKQHFNYDKSCFAKVFSDQLVSTPKPSLLQAEYQMHQLQDHGKLLSPSTLDDTTDLNLSVTEGAITGYGAFLELDLNTDLEPSSEFEESQLPQELQVFQQQDPHHPYQQQQQQQQQHISISNNRVLTLYPPKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNR0</accession>
    <name>A0A1E3NNR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_15640</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018844.1">
        <property type="nucleotide sequence ID" value="XM_019160903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNR0"/>
    <dbReference type="STRING" id="763406.A0A1E3NNR0"/>
    <dbReference type="GeneID" id="30177590"/>
    <dbReference type="OrthoDB" id="2516at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="302" mass="33842" checksum="7C0C797A366A7342" modified="2017-01-18" version="1">MLYMIGLGLAHESDITVRGLAAVKKCKRVYLEAYTSILMSADKAKLEEFYGREVILADREMVESESDKILENADVDDVAFLVVGDVFGATTHTDLAIRARDLGIAVESIHNASVMNAVGACGLQLYNFGQAVSIVFFTEDWRPDSFYNKVMENRRIGLHTLLLLDIKVKEPNIKEMMRGRIVYDPPRYMSVSTCCEQLLEIEEKRKEEAYTPETPCIAVSRLGSPNQSFKAATLKELAGYDAGEPLHSVVILGRQVHDLELNYLLAFADDKEAYRKSVIADQEYFKPPPPPPAPEEIISDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNR5</accession>
    <name>A0A1E3NNR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LDB19 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_21011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018854.1">
        <property type="nucleotide sequence ID" value="XM_019162180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNR5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNR5"/>
    <dbReference type="GeneID" id="30178867"/>
    <dbReference type="OrthoDB" id="3832628at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024391">
        <property type="entry name" value="LDB19_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13002">
        <property type="entry name" value="LDB19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LDB19 N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="510" mass="56055" checksum="02A36574AB71550A" modified="2017-01-18" version="1" fragment="single">VKKVSLHLIQTVKYGKPFVPESSTLQNCAGCTKRVTELAHWDILSKKTAFAKGSSHSCPFSHVIPGSIPPTTILSNFNTSIKYELICLATFVDPKTGKDQLINLAQPIKISRSILREQDRNSTRIFPPTDVTSTAVIPNVVYPRSSFPVEIRMDHVSTPKRRWRMRKLNWRLEECVCVRANHCDSHNEKFSSVYQTMKKQKKSVKPSKNSGGLGHANVNYYFETPKSRLNNNNSSIASSNRTNERDPANPPTDDLFPSTSGSNHGSQPLAPSASNWSVPASPISSNLGSSNTLDQKQTEGIFVEEIRTISAGELKSGWKSDFSGCGRIELVVEVSLMNLISMGINNTIAYKGSINSQTCNLTFNELYEDANSGCNCSIDVDDPELGISVTHNLILELVVAEEMMQSTMASANQSAKARQHQQQTHGHQEDFPDDRVGPEYATGNKTDVSNHMQGIPTGVARVLRMQFKVILSERSGLGVAWDDEVPPTYHAVGALSPPTYDDSASNVTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNT1</accession>
    <name>A0A1E3NNT1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018849.1">
        <property type="nucleotide sequence ID" value="XM_019160907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNT1"/>
    <dbReference type="GeneID" id="30177594"/>
    <dbReference type="OrthoDB" id="10390067at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="111" mass="11889" checksum="FAF6CD12F9EE9172" modified="2017-01-18" version="1">MSQNLAAIRKNESNGVLSNTPSLSNLYNSTSQKGSNTTLAMTNSAVFNNGAGFVQKGTIAHINNTQHANSGANGDDHHGAEDEDDEDEAFELNWKPQVSSDSHTSGYYDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNW9</accession>
    <name>A0A1E3NNW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018885.1">
        <property type="nucleotide sequence ID" value="XM_019164012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNW9"/>
    <dbReference type="GeneID" id="30180699"/>
    <dbReference type="OrthoDB" id="4061106at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="362" mass="40621" checksum="5A41B109AC4227B4" modified="2017-01-18" version="1">MLRQSAHVRRFFATSAISRNSSSSSSSSKSSSLYAFKSQLDDYIYYHSLSSQWRPLVYRQRNAEVLTSLNAVDKKNNPLTPRQYPGVPSKKKLIKFISLLLTAEEVKLVRSSLEELSKIRISSKTRKGSTKKDSNKGKYLDPSVLNEFLYKSYELDSKLFTENLVWADQIREEDSVWSVKNTEAVAFVRSMIIKTSFPANPEVNFQTMVEKMKHWAAKAEMSETGSILYNASLVVASVYSNSLKENSTALNHLDALTKNKVYTVKPQTDYLQYDHAYSIISALKDASKHEYAENNAQLLELVSRWSTFLADVENLKQNTPSTYERFLSAEEGKRAAAATATAGAAAEPAPEQESADAAKAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNX5</accession>
    <name>A0A1E3NNX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15691</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018907.1">
        <property type="nucleotide sequence ID" value="XM_019160923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNX5"/>
    <dbReference type="GeneID" id="30177610"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="250"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="553" mass="61122" checksum="13E5620789EDC57C" modified="2017-01-18" version="1">MSTVYVHPTAGADAASATGAEGSPYQSAAYALYVCQADAKIFVHKDATEESDAGYYEISASALKKAKKGAEGLKKKEEKAKKQAEEKAAKDAEESAKMLEAMKITIAEDKSLPTAKTIKLAKISKNIDERVKVQGWIHRLRVQKGVAFIVLRDGSGYVQCVLSGPLANAYQTQQLTVESTVAIYGTIVKLPEGKSAPGGVELVADYYEVIGLAPGGKDSFTNQVAEDADPSLLLDRRHLTLRGETLSAVMKVRAALLASIRRVYQEEGLCEVTPPCMVQTQVEGGSTLFKMDYYGEEAYLTQSSQLYLETLLPALGDVYCIQESFRAEKSHTRRHLSEYTHIEAELSFLTFEDLLTHIETVITKTIQYVLEDPVAGPLVKQLNPGFVPPQMPFKRMEYAHAIEWLNENGIPNEEGQPFKFGDDIAEAAERKMTDTIGVPILLNRFPVAIKSFYMKKCVDDPLVTESVDVLMPTVGEITGGSMRIDDIDELLAAFKREGLDPAPYYWFVDQRVYGTCPHGGYGLGTERILAWLCNRFTVRDCSLYPRFTGRCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP14</accession>
    <name>A0A1E3NP14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Asparagine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15718</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018947.1">
        <property type="nucleotide sequence ID" value="XM_019160936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP14"/>
    <dbReference type="STRING" id="763406.A0A1E3NP14"/>
    <dbReference type="GeneID" id="30177623"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070145">
        <property type="term" value="P:mitochondrial asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000016">
        <property type="entry name" value="Asparagine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003037">
        <property type="entry name" value="PRK03932.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="168"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="500" mass="56451" checksum="E7FF41BE4FA65E56" modified="2017-01-18" version="1">MLSHRLARISTRPTKNLVNRVRRLATKPDAAKLDLSPCVKGILADVQNPDTPIEINGWVSSSRISKNVAFLDVNDGTTYEGIQCVIRPPSLLPSGIKNGTGIQVKGIWSEGKGKQKYEVHVNDTSQGKVNVLGPVDELFPMIKSNHSAQYLRTIPEYRWRDQSLAAILRFRSQVETTLVNFFDSQMFIKTHPPLVTASDCEGAGELFKIESSSKLKNDEQFFGKDAYLTVSTQLHLEVLCAALSRVWTLTPCFRAEESDTNRHLSEFWMLEAEIAFVDNVSQLTKFSEKMIKSVVTNLVADYNGMGSNLINTVDKSTTQIMTDRWEMLLSKDWDSITYTEAIKIIQNAVDSKSVKFETRPIWGDSLKSEHEKWLAGEYFKNPVFVTDYPLEEKAFYMKINDRTTEEAGTTVACFDLLVPDIGELIGGSMREHDLQTLKTEIERRGMDTTPLDWYLKLRENGSVPHGGFGMGFERLLQYLSCTENIRDVIPFPRSASTCLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP53</accession>
    <name>A0A1E3NP53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SIMPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_178399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018982.1">
        <property type="nucleotide sequence ID" value="XM_019161770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP53"/>
    <dbReference type="GeneID" id="30178457"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133458" description="SIMPL domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19792" checksum="2EF71FC26663BD7A" modified="2017-01-18" version="1">MKRVLFSLFCSTAVFWARSFSLYRLRVRDNTEEKNGPVKTEWWFVLSFYESIEENISCDSKILNIKMKGSIDKELLKRGIDDAIFMTAAADTRQLALAAALERARNDLLLEEKKVIIEAVENEENESYRRSRIRTLGFIGPQEFSPDETAAFDKIPKTIRYAEFLIKKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP61</accession>
    <name>A0A1E3NP61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GIT Spa2 homology (SHD) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_71903</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018999.1">
        <property type="nucleotide sequence ID" value="XM_019164044.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NP61"/>
    <dbReference type="GeneID" id="30180731"/>
    <dbReference type="OrthoDB" id="5588096at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902716">
        <property type="term" value="C:cell cortex of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036267">
        <property type="term" value="P:invasive filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039892">
        <property type="entry name" value="Spa2/Sph1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601:SF0">
        <property type="entry name" value="PROTEIN SPA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601">
        <property type="entry name" value="SPA2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="4">
        <location>
            <begin position="35"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="4">
        <location>
            <begin position="90"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="199"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="765"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="835"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="963"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="997"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1030"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1054"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1257"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="323"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="443"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="855"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="879"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1084"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1138"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1182"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1257"/>
            <end position="1271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1438" mass="159927" checksum="2064EC8C31255A1C" modified="2017-01-18" version="1">MSSQEWPRYQEALEGFLQSSQGNLTPATSSRVAKAREKLKKLTKIQFSDLSTDVYDEMQRRESTRLDPENTSLPKYLASQPNYHPKRNQARQKLAALPPSRFKDLVNDVLFEIGNRMHKEDKVVSKTKNQKSGGNIEREKDNITSQESDVADIHNNNNDGPFDTQPDFHASGHANGTGSDPTGNLKIDVSKTQQFNDDFFSDLKTPQTSDFLGTTKSPATPSQREIKPTTLVPKKSELTWSSDEDDDREDQQDTNHNANISQEDLVNATHNLFEKDIEKSHKPEEADFDTINRSPEKELNFGNNEILDTHTLRHDESNTGTDSTSLKNELQELRFKIDELEHENSHLKALATQQQELQSENFNLKSQLENLNQNHRDVNNHLENMTNQFADYDEMKQELYDVKGKLSAQSEELDTLRQMTDIGQEKQLSVEDSREFDTLKTYLDKVLEENEELKSRLATITSTNSDNGAKHLEELENLREQHEDLSQSHKILNENYKILKTHSDDLSKTLEKHQANATTAATKNVINTNIQANTSAVLDWQNKFETLRSNHIQLKLSSLSQLPKFADKKAFSPNGLISIQSISNVNASLETILIYMDSRDDSDESLIDPSVLFERVANFVSNANVLSKEVVVSSNDRNYVRIEEKKRILKNAISNALSTTKHFALYRHILPQLVLNAALNDVYFSICSLISLAKIRGDGTKGVPQIKTDYLEDNATITATPVALKKISEIEERSPSYGSIQAANNTETSVRPLRITQRLASGSTLDLNEFSKPGQPNGSRSGSPMGRKILGSPILPVIAAGKKNSEIDTVKLVPNVKRGVTRNGSVENIKDSVIKSGLNSPVDGESPLDNVKQFSNSNNSKLSVSNLASKFSSPEHENNSTTSVSKSNAPSPSRGKNIVDKLKKFDTSVDDLSIASNGKGSPSSSKVKVGHDVAKAFDKFGAKRRSVDLNSDVIAKDELREHSKENNIDNSEKSLSKETLKDSSKVLHNKDLNTVNDVANRNPFIDGSVGSKKGGLFSTDDQTDMSLKQRMKESANSGLETEADDENNEKDVFGLDKEQHTKNYTGESASDLKLNSEGCSSKEVLPEHESSLRVSKEPLTDIKSEKSGKVLVQADNEGKSKNVDNISNVVGDDVETFDGDKYFNAPDHEKKEKNPSAASEESLPELKAPYSAKSNAKVEPLNLNSGTSSEKLNGIDAKTNDAEEDEDFNNYGTTKDVLAVNDPLPSAGSALDIDENLVRRVSKRQSYRKSVLTNNVSAQPDTDKQGWNYNGESEEEEEEEFDVDKFNTLNPDNTLRELLLYLEHQTVEVIGAIQKTLQSIRDPKATKGLLRSGTAEINSVVKQMAEGTSTLMNQSRYSESMGHAKYVVGVLEDCVQRMETLYGKDTSSDGEYAGKNFKQRSAGIAFDVARSTKELVKTVEEASLRDEIAVLDSRLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP80</accession>
    <name>A0A1E3NP80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_15761</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019000.1">
        <property type="nucleotide sequence ID" value="XM_019160951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP80"/>
    <dbReference type="STRING" id="763406.A0A1E3NP80"/>
    <dbReference type="GeneID" id="30177638"/>
    <dbReference type="OrthoDB" id="360161at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048254">
        <property type="term" value="P:snoRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="151"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="350"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="552" mass="61879" checksum="A0BD2AF5237531AE" modified="2017-01-18" version="1">MDIFRVLSRGASLNKSKDYKADFTAPDKVKSATELKKSNDLNVQVEKEIDFFHSKQHKIVKEVESKVNGDESDDDVKDDETRKQEPVKITTKEEALELRKLNGSKVTGHDVPLPIGSFEDLISRFRLSKILLQNLIANNFNEPTAIQSEAIPCALFNRDLIACAPTGSGKTLAFLIPLVQNILASNIRSKKRKGGENKGSEEKRIKGLIISPTKELAQQINNQLNKITENLGIKTGYLNKSLASKFRSGVSKASKFDIIVTTPLRLIDLVKNSALDLSYVEFLVFDEADSLFQSDFLEQTDAILSALTSPKLCKSIFSATITSHVEELAHGIMKDPVRVIIGLKEAANSNIEQKIIYCGDEHGKLVAIRDMIQNGGFKPPVIIFLQSIDRAKALFNELLYDGLNVDVIHAERSQKQREDAIARFRSGELWCLITTDVLSRGIDFKGINLIINYDVPNSSQAYVHRIGRTGRAGRTGRAVTFYTKEDTDMIKNVVNVMRASGQIEGMPIWMRDETALSKLSKDRKRKLKREQLERGKISTVPKAFKKRKFRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPC2</accession>
    <name>A0A1E3NPC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_121751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019073.1">
        <property type="nucleotide sequence ID" value="XM_019159893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPC2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPC2"/>
    <dbReference type="GeneID" id="30176580"/>
    <dbReference type="OrthoDB" id="4096851at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="574" mass="65103" checksum="AACE028493F6E81D" modified="2017-01-18" version="1">MDEFTSGVESATVSVQSSIIKPRFRDEDTEFSTMGMYEDDDIFHQATASVKFALPGSNRTLFSRSNYYTGLFYTFLRYVLSLWFTMYQYYLWIDSQLKEWCTITTSLMWQLLMLIPFPEDVSVVGPASVQSATSVRTIKSPSSIKARVLDSFNQTRMLPTKIGNLLLEKSLLASVPRNRLQIRQVTGRLYVPEHVTFIFEMSALVVPQPPEVPVPFLDYEKRIVVPEKKEVAKVLAKRAEWSSLHHTHKAAETFRVIYEAAKCISWAACSGVRTVTVFESNGYAWKDMQKMLGVLAEELKNLTLESYQVSDSIKLVNLDTGEIFRAYDQEETFSHDSAKIQIQTEPQTKSYNDDGEKQGADVLVDNVKLRFREQEHAAEKEEPEVFMSGVHTSELSEEHNGIERRTGISELEPVPESIVDDSVGIDSMDPGSADLCHTFRTNLTIFFMSNKTTNPAAFRALQIKQEVKKRLSDSSLTTALADSEDEFVHYPINPDYTVSYTDPELIFKFCTSNQMPYSLSGYPLVVSNDSNYGPTTFPVFLSESRPANFPQFVRGLVRLNSVLREDAKNSCSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPU7</accession>
    <name>A0A1E3NPU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="8">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_51367</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="8"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019264.1">
        <property type="nucleotide sequence ID" value="XM_019163153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPU7"/>
    <dbReference type="STRING" id="763406.A0A1E3NPU7"/>
    <dbReference type="GeneID" id="30179840"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160203">
        <property type="term" value="P:mitochondrial disulfide relay system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="10">
        <location>
            <begin position="64"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="174" mass="19825" checksum="714861F85FB0797F" modified="2017-01-18" version="1">MSEEDNPVKNPAKPNFGASGRKIVYDENGKPCRACNTLLDFRMVTNKNIAAAAVANDAANARNCPPDVEELGRSTWTLLHSIAATYPDKPTKSQQENLSQFMNSFSNLYPCFHCADDFREHIKKDKIKVTNRDEFGKWLCNAHNEVNVKLGKPKFDCNLWKERWKDGWKDGRCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPW1</accession>
    <name>A0A1E3NPW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_58395</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019195.1">
        <property type="nucleotide sequence ID" value="XM_019163295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPW1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPW1"/>
    <dbReference type="GeneID" id="30179982"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="5">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="135" mass="15709" checksum="F6A814A717C0E3CC" modified="2017-01-18" version="1">MTQGDELSLSRYMDIFATAEKFKNPNRVVKKVRYKGARLVLNEESRRLNQLQEEEKTLHPDQPPKDRTTYFSLAAPPSLRPIRTYCDITGLPTNYKSPHNQIRFYDKECYEIVRSMPAGVDQQYLSLRGANVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ54</accession>
    <name>A0A1E3NQ54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MTHFR SAM-binding regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15731</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018961.1">
        <property type="nucleotide sequence ID" value="XM_019160941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ54"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ54"/>
    <dbReference type="GeneID" id="30177628"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="308"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="614" mass="70149" checksum="48E11F34F816A2C5" modified="2017-01-18" version="1">MKITDKLAQAHKDDKAAASFSFEFFTPKTSQGVQNLYDRMDRMYHLNPLFIDITWNAGGRLSTLTSEMVHTTSTVLGLETCMHLTCTNMPLQLIDDALQKAYLSGCQNILALRGDPPLDGSESTGDFKYAKDLIRHIRAKFGNHFCIGVAGYPEGHPEEPDHAKTLSYLKQKQDEGADFVVTQLFYDVDNFIDWCARCRGAGITLPIIPGIMPISNYNAFLRRAKWNNVAVPQSFIDRLAPIKEDDSRVREEGCLLMAELCQTLLDSKHVNHLHFYTMNLEKSTTMLLDSLHLVSNQTKQDYECENSKPWRKSLNPSRELENVRPIFWKNRKFSYIQRTNNWDEFPNGRWGDSRSPAFGSLDMFEHNIIRHSPTKILQLWDRPTNSHELGELIVRYLNNEIHCLPWSDTQATEEIDTIKPDLVQLNMNGVITINSQPKINGVKSTDHVFGWGPTNGYVYQKQYLEFLLPKTKLAKLVSQITAVNEENGYDTLNYFVTDAESNKLSETNVTDLSDVNAVTWGCFPGREIAQPTIVEKVSFLAWKDELYLILKKWSNVLLASSSDEEGEPDEANRLRDLKSSNFIQSLIKDYVLVNIVDNDYIQPSDTIFHILKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQJ9</accession>
    <name>A0A1E3NQJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Vacuolar fusion protein MON1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_13935</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 4">Prevacuolar compartment membrane</location>
            <topology evidence="1 4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MON1/SAND family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019458.1">
        <property type="nucleotide sequence ID" value="XM_019160142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQJ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NQJ9"/>
    <dbReference type="GeneID" id="30176829"/>
    <dbReference type="OrthoDB" id="272411at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF7">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="5">
        <location>
            <begin position="102"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="6">
        <location>
            <begin position="275"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="7">
        <location>
            <begin position="480"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="622" mass="70878" checksum="4681F067CBCAD2A7" modified="2017-01-18" version="1">MFKELRTVPMNSISLENVSLNRMNGAENSTNDDVLSVNQADDTASTILSPIITAANITDDVENEPTANSDFKETGFPFVLNECLEYSDDSDNISKFFGNRQHFFMLSSAGKPIYCMHGSYDIFVVYSGVIQTIVSFYKYSSNGSKSVKIIECKDKKTGQPIKFVFLDKSPIILMTIVRSNQSTYIELEQQLDFIYSFILSALSKPYIDKLFGKYANFDLRNLLGKTDVLTLDSICEGFANNLNVSQVLGGLQCLRMHLSIRSRLERKLMNFRSENLLYGLIVGPNEKLISIMRPKRHTLHTSDLMILFEMIFNTNTFKTKSDDKGQLKFITSETFWVPICLPKFNSSGHLYNLIQFHQLKDERLFKLHDIKDTDDLHLDEDSTKIGIILMSPYKDTFNEMRKISNEIAKTILFDHKIYRNIWSSLVGNGRIVVDKIIGDKGNEFKENVQGSTAFSSLINKLSLNSPKQKNLSDGTEPWSGILHFAVRSKRSVQCIFPESVYFDINDEKVRRNLLNVYKYLRHRLHVVWTDTACLDFSEGSEKIVLDGGEAAGGSNTVIYEDWYDEINEEKILGFGCKFGTYEVFIIGKGTVTESDMLAYGLKVVKWVRKQDSRIFISTGCIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQX3</accession>
    <name>A0A1E3NQX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">AdoMet-dependent rRNA methyltransferase SPB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_25879</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019569.1">
        <property type="nucleotide sequence ID" value="XM_019162416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQX3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQX3"/>
    <dbReference type="GeneID" id="30179103"/>
    <dbReference type="OrthoDB" id="1287559at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000004">
        <property type="entry name" value="AdoMet-dependent rRNA methyltransferase SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03163">
        <property type="entry name" value="RNA_methyltr_E_SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012920">
        <property type="entry name" value="rRNA_MeTfrase_SPB1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024576">
        <property type="entry name" value="rRNA_MeTfrase_Spb1_DUF3381"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028589">
        <property type="entry name" value="SPB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11861">
        <property type="entry name" value="DUF3381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07780">
        <property type="entry name" value="Spb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="9">
        <location>
            <begin position="25"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3381" evidence="11">
        <location>
            <begin position="235"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA methyltransferase SPB1-like C-terminal" evidence="10">
        <location>
            <begin position="607"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="482"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="578"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="500"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="604"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="614"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="94894" checksum="E16BEA15A86F8200" modified="2017-01-18" version="1" fragment="single">MGRVQKKNAKGRLDRYYYLAKEKGYRARSSFKILQINEKYGHFLEKSKVVIDLCAAPGSWCQVASQLCPVKSLIIGVDIVPMKAIPNVITFQSDITTEDCRSKLAGYMKTWKADTVMHDGAPNVGLNWVQDAYTQSQLTLQALKLAVEHLNVGGTFVTKVFRSKDYNNLMWVFNQLFEKVEATKPPASRNVSAEIFVVCKNFKAPKKLDPRFLDPKHVFEEVEDKKNNEAKVFNPELKRRSRQGYEEGDYTLFHPMKLMEWVKQEDDIITTLGSISMFEIDTEDPEWKILKKMKQTTTEFMECCKDLKVLGKKDFKMLLRWRKHARDELGILDPKDVKTSESLIEVEELNEDQKIDKELDQVKDRLRLKQKREKRRSNLQKQKEIQRMQMNMLTDMQIGIDAANIGSETLFNLKTAEKTGEMDKLAKGKKTIVFNNDHNHLSHDSEMVLEENAGSRQLIQREDEAENLEEELEDMYASYKDRKKERDAKMRAKEARGGDDDAWDGIKEDEEVVSSDDGEEQEDDDSDEDEDSEDDEAINKLIATIERKKKNSLSTKASNFFSDSIFQDVDLNIASNIKGDTKILGSEAKTKMKPLIPAPKQSKKKSDEKKEMDSETESEEEGENSDDEFEIARESHKADIDIATAQAMTMAHELALGKKNKKDLINDGFNKYSFRDKEGLPEWFIEEEDQHSKINRPVTKEAALALKEKMRQLNARPIKKVLEAQGRKRMRAMKRLDKLKKKSDGIMVDESKSEADKAAEIQALMGKMTKKQKTKPKVTLVVARGSNKRLAGRPKGVRGKYKMVDGVLKNEMRSLRRIEKKHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQZ3</accession>
    <name>A0A1E3NQZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ GRASP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70118</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019599.1">
        <property type="nucleotide sequence ID" value="XM_019163560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQZ3"/>
    <dbReference type="GeneID" id="30180247"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF0">
        <property type="entry name" value="GRASP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="167"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="354"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="385" mass="41680" checksum="2FA4162F0C252480" modified="2017-01-18" version="1">MFGFAKKLVNSIESQLSYDGSSDHNSRVDESRQGLRIQSVKPNSIGSDHGFESWFDFIVGLNGTDICAYLQLNTETGQVNYENMLNYIKHEVEVSDNDLTFSVFSSKGSVLRDVIITSAEVKDSLSKTTPPLEEVSLSADSTDTIGLQWTPNLGISFQLTPLTTGFFMWHVLRVHPGSPAYLAGIIPDEYIIQSQDGLLATGGEDLLAKVLQSQYAKAGDGCEVVLYVYNYDSDCVRPVRVILKAGSSWGGRGILGCDVGYGLLHRVPEVIGKFQTKSVDQHLPGSASTVTELVPEVPLTQPPEFLPLQVTAPSFKSDGVGESVDEAAKSVVSSTHRKRGHQSAHTAVNLEAYFDEQTEKSKAVDSASTKETGSVPPPPPPPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR16</accession>
    <name>A0A1E3NR16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_29411</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019680.1">
        <property type="nucleotide sequence ID" value="XM_019162590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR16"/>
    <dbReference type="STRING" id="763406.A0A1E3NR16"/>
    <dbReference type="GeneID" id="30179277"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000008">
        <property type="entry name" value="Glutamate carboxypeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA" evidence="3">
        <location>
            <begin position="139"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="5">
        <location>
            <begin position="323"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="4">
        <location>
            <begin position="608"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="726" mass="81831" checksum="0CD0A0B25D5F3B73" modified="2017-01-18" version="1">MGLALWKLGPSEAALPFHDSYGGSATTYGWASSILLSSLETNLAGEWLKDYTADNQLAGTNIAMVNYTLAKFQELGLEDAYVDQYTAYVSYPLDNSLSLIDNSSAVVYKPTLIEDELEEDPNSSYYVPAFFGYAASGNVTAEYVYCNYGTYKDFQALRELGVDLEGKIAIIRMGAIFRGLKVKFAQEQGMSAALLYTDTADDGEIVEENGFKPYPHGFARNPSSIQRGSVTFGLAGDPTTPGYAIKPGEKKPREDPSGLPKIPVLPISYRDVKPILEKLSGHGPKIDGWEGRIKDYDYSVGPNANYTLNLYNKQEFNISIMNNIMGKITGLDDSKFILIGNHHDSWTPAAADPHAGSASMLEIIRAFGDLVKTGWQPKISIVFASWDGEEYALLGSTEYAEYYQHELKRKCVAYINTDAAVIGPILSLEASPLLNDLLLETTKELQYPNSTITLYDHFMDKAGGKIGTLGSGSDFTVFLEHLGIPSVDLNFGNDPTKSSVYQYHSLYDSYYWMSKFGDPGFVFHNMMAKYISLLILHLSDEPVLRLRTHNYAVELQKYYHDLEIPSQWWNQTQGKHCGHGGSPSKESFETKKKYHPLKKLIKKVDKKMQVLELKTAEFDDYLWELVDMYDHWDSLTYWQRIKLYFKTKGANSVLQYFERHFLDHKGLNNRPWFKHVVYASGRYTGYKGQELPGLAEAIEDYNLDDFTERLGKLSEILDTLLRMCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR94</accession>
    <name>A0A1E3NR94_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent RNA helicase FAL1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent RNA helicase fal1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_14155</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019728.1">
        <property type="nucleotide sequence ID" value="XM_019160248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR94"/>
    <dbReference type="STRING" id="763406.A0A1E3NR94"/>
    <dbReference type="GeneID" id="30176935"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18045">
        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="54"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="235"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ48615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="396" mass="44554" checksum="E7F59B81C848AE08" modified="2017-01-18" version="1">MEFNREADSELKFKTSKKMPVTSTFEAMGLKESLLRGVYGYGFSLPSAIQSRAITQIISGRDTIAQAQSGTGKTATFSIGMLEVVDTSNKSSQAIVLSPTRELATQIQNVIRALGDFMNIRVHACVGGKSVGDDIKALSKGQHIVSGTPGRVLDMIKRRNINTRSIKMIILDEADELLGKGFQDQIYDIYRYLPPGVQVVVVSATLPKEVLNMTKKFMSDPVKILVKRDEITLEGIKQYFVQVEKEEWKFDTLCDLYDSLTITQAVIFCNTKKKVDWLTSELKRANFTVVSMHGDMKQDERDKIMNEFRLGNSRVLVSTDVWARGIDVQQVSLVINYDLPTDRENYIHRIGRSGRFGRKGVAINFVTEEDVAALHELEHYYSSQIEEMPVNISSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRD6</accession>
    <name>A0A1E3NRD6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019744.1">
        <property type="nucleotide sequence ID" value="XM_019160256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRD6"/>
    <dbReference type="STRING" id="763406.A0A1E3NRD6"/>
    <dbReference type="GeneID" id="30176943"/>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="38"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="791" mass="87594" checksum="415DC8FB2641EA71" modified="2017-01-18" version="1">MSTSSQSGDEDQNAFLAKIPHPLSSSGPMISSSQSTSPLNLNGFPLEVLIIVLSYVDPLTLRNIPLVCKTWNSILQDDTIWNAIFKLQYPYKREIFSSVTRSTQFKTELLHRTQLRYNYRRGRLLTQQYTTNHFVSGSSIAIDWSRNRLTIIDLPRSAILTCDIRNGKSPKFATDFVPEGVTSYDAGNTSGSLHGSRVLVFGRWDGSVSGALIDWKGLVLSDVHNWGSMSGGRVTCVTACVNSSISTNFAAEKKANLPPISSLTLSSPSSISSSTTTLSSTLKKNIITSPPSSSHNILTKSGMAGAFSADETGTINAWDIRTGECLCEYKTAVNSKIIKLQSDGKTAVIVLFQNGELWLLRNVFGSLKGDDVKVKFEKIGQVPFDRNGLITLNLFIDYGGESVVVWNEIEVLVLSYSNISEASRQVQNHDNIISYTPPERTLISSVSFESNDKLFIKRDPKIVGSDPLLAAVLLNNGIVDVINIREPPSSTALEPINTIVPKFLSEHESGVSMNYSDTRTSPVASVCLNSLVLAISNHLGKVELFDVMTGEYLRTAIDRVGKKKLQELEGLLPNRLINSSIKVYLDDSSTRGVLIIGSHIQYFLCGGPKNDDDATGVKKKTKSGKRGNDKKGDFSEDMKHSMSVYEEEKMQKKKERELLAKYNGEGLDDEKDQLNMAIVLSMSLNETGNKREDYNLNGNLNESSDSYHEDEEMRQLNEALKMSLNTTETTETTPIENHSLADSNQQTNTETETDPDIETDEDLLLAIELSKQENNMDWEAVEDRWESLVHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRE7</accession>
    <name>A0A1E3NRE7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_85093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019752.1">
        <property type="nucleotide sequence ID" value="XM_019165012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRE7"/>
    <dbReference type="GeneID" id="30181699"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="233" mass="25162" checksum="29D0E37197F395D1" modified="2017-01-18" version="1">MGCGLRQPPIAAYTASGSPHGGRGGVRTPVRLECTADGVSSSIPEVSGFRQILISGSTREKVQHSETGISVPKKTQIKQAEGGYAYFPRCPTLRIRINLLYLRTAPYLRNAVPRGPRTHRRISTLLDCGCMYATKICSMLCYLSKLLASVLSESSVFPPGSGSQTGRRTLTTALQPSRRIKRRSQPGAATCVPRHCTRAGAKTRALLSPWRAEAPLPRTTTSANATDPRAVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRL8</accession>
    <name>A0A1E3NRL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 17">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_16014</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17 18">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 17"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019316.1">
        <property type="nucleotide sequence ID" value="XM_019161057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRL8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRL8"/>
    <dbReference type="GeneID" id="30177744"/>
    <dbReference type="OrthoDB" id="10251079at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000003">
        <property type="entry name" value="CDP-diacylglycerol--inositol 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="16 17" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ48203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="228" mass="26007" checksum="C32FEEA23B071799" modified="2017-01-18" version="1">MVSLTEVPTPVTPEAIFYFIPNLIGYGRVITLILSFFTMAHHPLITMGFLYSASCLLDAFDGYYARKFNQSTQFGAVLDMVTDRCSTCSLIVFLGVLYPDYFIAWQALISLDLASHYVHMYAQIVSGSTSHKKMREGANWLLKLYYENRVFLFLVCALNETFYIALYLHHFDFLYVPGTSVSLAYWLTLLCSPVWFFKQICNVIQLVNACQMLAQLDAQNMNAARKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRY3</accession>
    <name>A0A1E3NRY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 8">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_29240</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 8"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019978.1">
        <property type="nucleotide sequence ID" value="XM_019162579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRY3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRY3"/>
    <dbReference type="GeneID" id="30179266"/>
    <dbReference type="OrthoDB" id="10248475at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071244">
        <property type="term" value="P:cellular response to carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00883">
        <property type="entry name" value="beta_CA_cladeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF76">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00705">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="253" mass="27498" checksum="0E1E03936D0A9ADC" modified="2017-01-18" version="1">MNESASTATAPAAAAAAAVSCSSLGHTHSDSYPFTLSKNSTLKDYLAANRRNMDKLKAEHPAVMPLSGKGQQPHTLWIGCGDSRINECTALGCLPGEVFTLRNIANVINNSDVSSMSAIQFAIEVLKVSKVIVCGHTDCGGVWASLSNKKAGGALDQWLSSVRHVRAQHLDELKAIDDINDRCKRLVELNVLNSVNTIQKNSSYVEAHARGDVELYALVYDVESGYLRELPLDHHDDYSEVFRLHENSDAPVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRY8</accession>
    <name>A0A1E3NRY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_9318</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019917.1">
        <property type="nucleotide sequence ID" value="XM_019165087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRY8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRY8"/>
    <dbReference type="GeneID" id="30181774"/>
    <dbReference type="OrthoDB" id="28322at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133481" description="Glucosidase 2 subunit beta" evidence="5">
        <location>
            <begin position="18"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="6">
        <location>
            <begin position="419"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="523" mass="58323" checksum="3CE01666F8CEABFC" modified="2017-01-18" version="1">MLLGLSSLFLIIIHSHALEVLRGVSPESIIRYQPDENGNWHCLNDPSIVISFDRVNDNYCDCPDGSDEPGTSACSNGLFFCENVGFESHFIPSFKVDDGVCDYEVCCDGSDEAPGICENRCISMKATHEKVVNEHNKKITQGFAIKQKMAEKSKNMRSDIEQAISKYNHYIASIEENIAELELVRGKTDENEEKINGNFKIIETDLASLTSSLLDSLNQLGGYISKLESLKSVLKMMTEEYNHNFNDPAVKQAAQEYLNFAASFDEASDDSSDDSGTIQRITVEKVIVAFQESLKGINEDIAKVRSDIVALKIEQNEKEHSNIVDSSSTSIFTETWEIVAVGLKRLVDSFLGVESRIVSYEESIEPHKDTLKSDISNKDAANQIASLSTSLEGLKSEVGKSESELRKNYGPDDILRSMSECITSKIGSYKYRLCPTSVLEQINSDGRGTKIGVFEDLIFSEETNNYQFIFKRGERCWNGPVREAVVDVLCGTKQEILVVTEPEKCIYHLKMVSPLGCFESDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS40</accession>
    <name>A0A1E3NS40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 10">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_70477</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 10"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019995.1">
        <property type="nucleotide sequence ID" value="XM_019163651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS40"/>
    <dbReference type="STRING" id="763406.A0A1E3NS40"/>
    <dbReference type="GeneID" id="30180338"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.710">
        <property type="entry name" value="Glutamate cysteine ligase subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="665" mass="76000" checksum="61664D60304528EB" modified="2017-01-18" version="1">MGLLSLGTPLHWNDSKKYADHVRSNGVIQLLNSYKISQGRENDKFYWGDEIEYMMISLDEASRSLKLSIDHDYVLTNLSEDGKNYQAALDNDVLFHPEYGRFMLEATPLNPYDGTSLGDYFKVENNMHRRRFLAIKEIQSENVYPLTLTAFPLMGVDDFCSPSTLPKGCASKSLFLPDEIINKHVRFPTLTANIRRRRGQKVAINIPLYKDKNTRSLDAADPSIPKRELFPYSDNEPHLSAVNPAAKPGHIYMDSMGFGMGSSCLQVTMQAKNLSEARYLFDSLVNIAPLMLAVTAAAPIFRGHLADQDVRWNVISGAVDDRTPFERNEAPLENHPLYGGQTDVEVQKSMLRIPKSRYDSIDQYLGGTEFYKSSYNDLNAPINKKVYDKLLESGLDEELSRHFGHLFIRDPLVLFSERIEQDNETEMDHFENIQSTNWQTLRFKVPSQDSVPNSNKPGWRIELRPMEISLTDFENAAYSVFSVLLSRAYIHYRFNNYLNISLVEDNMKRAHHRDAINREKFYVRTNIEDSGDAYVQELTLDEIFNGSEKRKFKGLIPIVKDFVAVEFGHDANPDDLEKLDMYLELISGRASGATPTTATYFRKLISEHPDYKYDSIVPESAAFDLCLFAKKLSEYNPTTVRQFFGDKIGNWLIAHGYSSIFAKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSH1</accession>
    <name>A0A1E3NSH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70627</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020162.1">
        <property type="nucleotide sequence ID" value="XM_019163692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSH1"/>
    <dbReference type="GeneID" id="30180379"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="74"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="185"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="42191" checksum="1A4713275F35A96F" modified="2017-01-18" version="1">MSAFTKPVIISLFPIPEAILNHTQLLNKYDVVKYQLTTPSDFESDMSKEPYRSAIAIFGSYPGFKPIGGLEEQSLIDALPPNLKVIGLCSAGYDGYDLQHLSSKDIKLCNVPVDKYIAMDVADCALWHVLSGIRKFNSWDRLTRINTTEDNHSHTLKLRDKVRNDFVKPGEKQVGFAFGHIYHGQPVRRVSGRKCVIYGYGLIGKAVAERMITIGMEVNVVIRDKSKYQDSDFFFYSSSSPSEVEKATQGANVIVVCLPGGKDTFNCINKQTFDNVGSDCVVVNIGRGSCIDTEALKAAIESNKVSHVGLDVFPNEPIIEAYWHDEKEVNSSSNRFYTTSVTPHLGSSTIDTLEFASFTCIENIMIGIETGQFKNVVNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSQ3</accession>
    <name>A0A1E3NSQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020187.1">
        <property type="nucleotide sequence ID" value="XM_019163697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSQ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSQ3"/>
    <dbReference type="GeneID" id="30180384"/>
    <dbReference type="OrthoDB" id="69269at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057826">
        <property type="entry name" value="WWE_C20G8.02"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23463">
        <property type="entry name" value="WWE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="2">
        <location>
            <begin position="536"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="763" mass="87471" checksum="34A17F5BA0BF9DA7" modified="2017-01-18" version="1">MLAKGLMLQYGILKKSVFLGARSYSSSASFTVTTQAKPTRVRWFYATDIPNTKPYHPNYKLVKRPAKFLPFTREDSKRLEDSFYKLSKETNKETSKYRHVNVNEDGLFSADLVTKTIKPTYWIGPTYEIRRGIWFLDENQPLPDLLSEQVEENYRKYRPDHFLGKELEKGEKHQIPPIQLKSEKDIYESKLKNGMKRRWPFHEFDDLSDAPKILQFKSDNLALLVNKGQLLPQFILENLAGYSNSVFGLYTITRGYEEKMNQSNENQNKRGKSSSDIDKKSEADSKFGTMQELAEKDTKMEEDISEGFKNSSSETNHKFQKSMENDFSNASVITENSQDREVEHLILCVHGIGQFLSSKYASVNFAHDCNHMRQLLKTEFVKKSHQFAPLAYGEEKIGNDARFKNCKTQILPIVWRYSIDFGLNYLYNEYGKDGEYRLPKLSNLNIDAVTPLRNLTADALLDILLFYEPKFKEEILECVTKSANQIYDKYLANHPNFKGKVSLIGHSLGSSIVLDILSRQPDKIPTGAGFNAKEHLKFDVENFFSLGSPNGVFKFLERKNVYPRSFRSKSDKTDYKLGSESAGTAVYPKVNNLYNIFYATDLVAYRLEPLIHTSMSRVKPKNIRPINEENIITSKIKDMSKSSSDILSNKVVRTIVENTATWQGSLSTANLFNGKESIIETSDLVKDLMFGLNKNGRVDYVLPQGMFDIDMINAVYSHIQYFDDADVADLILNELWRKPEPNRDIIGVPNTAPQPTNPDLDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSW9</accession>
    <name>A0A1E3NSW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_70378</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019881.1">
        <property type="nucleotide sequence ID" value="XM_019163625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSW9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSW9"/>
    <dbReference type="GeneID" id="30180312"/>
    <dbReference type="OrthoDB" id="10251342at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000001">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="6">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="38347" checksum="061CE650F826F68B" modified="2017-01-18" version="1">MSDDLIWNVINKQFCSFQIRPHQSQNNQNFCRNEYNVTGLCDRRSCPLANAQYATVKQVDGKLYLYMKTVERAHTPRNLWQRIRLSKEYAKALRQIDEHLMYWTEFNINKCKQRFTRLKQVQITERRMALREERGEVSERNYVGVKHKVKKREEKRERKALAAARIEKAIEKELLDRLKSGAYGDKPLNVDDKIWKKVLGKIENRDTEEELENEEDYDSDEDDVQLESEDEDEDEGQIEYVEDDENDDEEMVDMEDLEKWLGHDSDASESDEDDDEDDDEDDDEAKDTATGTKSTQPKRKKIKTKRARIEVEYEEEPLTNIATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSX3</accession>
    <name>A0A1E3NSX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_57283</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020322.1">
        <property type="nucleotide sequence ID" value="XM_019163227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSX3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSX3"/>
    <dbReference type="GeneID" id="30179914"/>
    <dbReference type="OrthoDB" id="3998078at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133504" description="WSC domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="10">
        <location>
            <begin position="20"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="113"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="412" mass="42103" checksum="A07BC83E1385C0F1" modified="2017-01-18" version="1">MLMLLPFLLVLLSSINRSSCYEKIGCFQGSAISGLTEKGSYEWQSSGYCEGQCTGNYVIAVTNGNECYCGESLDQSQEVDSSHCSTPCSGYPQENCGGQNYYLVYVDNTMTPSSESSSSTSSSSSSSTSTSSSTTSSSSSSSSSPSSSSSSSSSSSSSSSSSSSSTSSTSTTPTSTSTSSSSSSSSSSFSITPSSTSSTAPLTTSSSSSSSTTEPVSLMTTESTMSSVYVSTSIENSSEMLVTIVHSVTAVSVQTKTATAVMTATIAPSATNSNLSSENKSTSGGVSHGAIAGIVVGVVCGTALLGLLLLWFCGVSLFCLPGRVRNDKYNDDEKSVRDDDSMSIHNGDKNAAIAGLGGFGRTPAYMYNADELSIGYGNRRFSQSSLPDAAAGSAGSESDNSNKRGLRVINPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSZ6</accession>
    <name>A0A1E3NSZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_98713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020378.1">
        <property type="nucleotide sequence ID" value="XM_019165137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSZ6"/>
    <dbReference type="GeneID" id="30181824"/>
    <dbReference type="OrthoDB" id="10681957at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="448" mass="50576" checksum="87B7D8DD19D9D98C" modified="2017-01-18" version="1">MSEKFYLNSDFYKISTDINEIQKTFEMIKLTDFTEIVQMERYPSNSTLLSLPLDPPRKRYRELYNKRNKLKTKSEPNLLKEAEPSKSYCGDDESCSSAKELSVHDIDKLESAEEIDLTYRSLPERALPKKISKTSHFYSKLKEGFLYKFGRHKREKEEKLDEESSRVKKPMEPPKSLLPKLLTSGTGNTNDELHRGITILSGKSYYFRNGIYLDDQRSEYQMSCPKTMQSLDKRSEIQTLILPETSMIPIRKDSQVRLKSLKYLPTSTNKSSHAGEFSESQEEGTEAIRSLSVDVASSSLNLSDANSNNFESSTNEDSMVSESNTRDRIDDTDGNSEYYDDDDQYDNDSDEYDDCEQLVESELEDGVATTTVALTGTTTRVSTVLIRSLLGTQSTSHITLLPLPLPALMLSLDQASSASNPSSTSSSGSSYCSILLSPEYLHKRTQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTE2</accession>
    <name>A0A1E3NTE2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_9438</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020041.1">
        <property type="nucleotide sequence ID" value="XM_019165093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTE2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTE2"/>
    <dbReference type="GeneID" id="30181780"/>
    <dbReference type="OrthoDB" id="5840532at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="335" mass="38095" checksum="74AB9A8F149D38E2" modified="2017-01-18" version="1">MKTFESFIPSPLVVQSGWNLDTLIYASLFTIFNPITVWIFYFTEKVSGRYATAIAIFWVCRYLNRCMRKSYATPSTNKLVLISGGASGLGSELVKKWSSQKDIGKIIVVDVQKPDKKTGCGCDKIHYIQYDLNDTSLRFLKTQNGIEHCDDFVDLSKVDILVCNAGIRQMQTLQELQAEEIRKISHVNWLSHMLLIKQYIEAISSREKSRRFQVVVVGSVLGFVGPKQLGIYAGTKNALMSLMDSLREELPSNIVLSTVLPGQLDSSMFADVSVNHFLAPIIHTKKLAERIHTIVEEGWNGTFSYPTYGRLLPIYRVLPWWAQRFCRWFSGMDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTK0</accession>
    <name>A0A1E3NTK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_70964</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020535.1">
        <property type="nucleotide sequence ID" value="XM_019163785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTK0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTK0"/>
    <dbReference type="GeneID" id="30180472"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000016">
        <property type="entry name" value="Solute carrier family 25 member 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="122"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="189"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="314"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="421"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ49422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="517" mass="58210" checksum="05059843D93ABBF2" modified="2017-01-18" version="1">MFPDESAEDKRKRHEQIFKSIDKNGRGTVSFAEFQRAMMEENHPLKDSPVAMAEIFQSFSEGTERSHFWSRRKDIDFNKFNEYLMTAEAQIERGFLNVDKDHDGLIKPVDVSNYLTNIGCKPKPEEIAKFFSNIDIDKKGYVTYAAFRDSLLFMPRIDGSRIRTAYKFFNDDLDYLSSEGDVTVGDNILNSVGYFLAGGLSGVVSRTCTAPLDRIKVFLIARTDLSSTLLKNKAELRHHIEELRHKKIPPQKIESPLVRAIKTLYKQGGLKAFYVGNGLNVIKVFPESAMKFGSFEATKKIMCQLEGVDDEKKLSKFSTYLSGGIGGVLAQTTVYPIDTLKYRLQCASLDSKIKGVNLLFRTASDLYKEGGIRVFYRGLLVGLSGMFPYAALDLGTFTTVKKWYIKNQSLKLNCPVDDVHLPNYIVLTIGASSGTFGATMVYPINLLRTRLQAQGTYAHPYTYTGFLDVLGQTVQREGVQGLFKGLVPNLAKVIPAVSISYLMYENLKVLFRLDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTM8</accession>
    <name>A0A1E3NTM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_9956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020604.1">
        <property type="nucleotide sequence ID" value="XM_019165154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTM8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTM8"/>
    <dbReference type="GeneID" id="30181841"/>
    <dbReference type="OrthoDB" id="3996906at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="367" mass="42842" checksum="BD6888E8A9EDFA5E" modified="2017-01-18" version="1">MNHERLMMIQSAAQAEPQRTRSSRGKGFPEDDSNKRREDRHRRHGHHGHHHHHRRHHKERTRQKEEGTEDRFCRFPSLKIELPPLSFPALGVCEVQEAPDFDTSCDELADDANPNQPQSHEPRSVVDRLRGEDGGIDLGLNRTDLGRVETESDYHNKKTRKMINYDPYGQQGKESTTSISHVACETTKLNRNNADDVDRIPFTFGDRGSTWRMIKLRKLDSGHARRPSKAQIFEQYATVWDYDLACMERDELESRKRKRENEWVYTPPESFMKKRKYHIMANKRAMKDVLRAKEEEEKLKHDDSEKAKGDDDQVVYMKKHGDVKVSKSQKLRNELKLAHLTSLDHAEPESYLSSISREQIESLTNSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTX7</accession>
    <name>A0A1E3NTX7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020612.1">
        <property type="nucleotide sequence ID" value="XM_019160607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTX7"/>
    <dbReference type="STRING" id="763406.A0A1E3NTX7"/>
    <dbReference type="GeneID" id="30177294"/>
    <dbReference type="OrthoDB" id="5592879at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="143" mass="16429" checksum="8AAC2838F0337383" modified="2017-01-18" version="1" fragment="single">MIEKKVKLNQYIQKLDTLRDNWNSINIEKIKQANESLRLHGDKKTNIKQSISDLSKLVSHKTKNVSDKKLRKERLLREVESLRKQVSSSTVPTKTDLKTLMSQLNSLEALKSIKLLPGASITLLLLDKLKAVFIKRGDDMYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU14</accession>
    <name>A0A1E3NU14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020281.1">
        <property type="nucleotide sequence ID" value="XM_019160465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU14"/>
    <dbReference type="GeneID" id="30177152"/>
    <dbReference type="OrthoDB" id="4088353at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="448" mass="50585" checksum="71703CDE43578CFF" modified="2017-01-18" version="1">MSSCSGTHDCKKLIFSQVMPPSITLATPAVSMTCVHSIPSSSTLSLQNTFSSDSISSFGSDSCSSSSDLVHKYSSPSLRSALFSKKCSLYKARKSSSKDLLSLISPPQTSSLQISQNCHCNRFHPAAEETSSNFSETTFASSYLSSFISDVDNNSLYSNPVSTTISTIYEVDDDSHPLGDFLEDETLAEVFDIRSNDANGHRNEYKIDKVSFTSLWDKLVSSSFITSTKLFYQSNESFFTNAVNNINIENRDLVLHDLLPLETFHTAYKAIDERHDRNNIIVPRSRECRINPLFLRFFAINESIKKNCSSELLDGSTVDYHQYEFINSGCSSLDEYLSSFNFEEKTTTLKFQLLSRDKMWSEVVLPPRSDISYNKNYKSFRDPYVKVKNKPIENGSLVRENGKVMPWLNLNDCAMQNKRCFSPSGTLPGNIQYTVKNWEKKRWSPTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU64</accession>
    <name>A0A1E3NU64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gfd2/YDR514C-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_22365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020692.1">
        <property type="nucleotide sequence ID" value="XM_019162251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU64"/>
    <dbReference type="STRING" id="763406.A0A1E3NU64"/>
    <dbReference type="GeneID" id="30178938"/>
    <dbReference type="OrthoDB" id="5953249at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040151">
        <property type="entry name" value="Gfd2/YDR514C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048519">
        <property type="entry name" value="Gfd2/YDR514C-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083:SF1">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21762">
        <property type="entry name" value="DEDDh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfd2/YDR514C-like C-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="199" mass="22736" checksum="1F14D83B05EF63E1" modified="2017-01-18" version="1" fragment="single">KKESRNIYLSVEEAIEVVNKREFTLVSIDNEFFERSTSKVIEIGISIYSPTYQKFALFPHFLNFHFIIKEFINLRNGLFVPDSKMNNITGQSLIISKRDVPRAMSLIFEILGPKVCIVGHNVSGDIKSFKYLNYELPSNIKIIDTMHLWYSFIGSSSGKSSLSFILDKLNIPNAFLHNGVNDAYFTLVVSLMLTSPELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND10</accession>
    <name>A0A1E3ND10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015168.1">
        <property type="nucleotide sequence ID" value="XM_019164902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND10"/>
    <dbReference type="STRING" id="763406.A0A1E3ND10"/>
    <dbReference type="GeneID" id="30181589"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03044">
        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000142">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="83"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="22043" checksum="8A2ED2055CD33A01" modified="2017-01-18" version="1">MTFGTLYYHPDYARSNWLVALGDYLGLTIEGKVAADSTEFPESFPLKRTPAFISSDGFKLTESLAIAFYIVNSSSKPEIAGKTVQEQANNWRWYSYLTSDMVNHVGSIYFAKTEEEKASAVETMNKNLEYIDNRLSDFKFLVGDTILVCDIFARSYFRMLQHFKVDFSNYKNIENYVSVVAKHPIFSETKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND79</accession>
    <name>A0A1E3ND79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_56462</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015192.1">
        <property type="nucleotide sequence ID" value="XM_019163188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND79"/>
    <dbReference type="STRING" id="763406.A0A1E3ND79"/>
    <dbReference type="GeneID" id="30179875"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="11">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="674" mass="76629" checksum="075047D80E8C43E6" modified="2017-01-18" version="1">MQHREEARRKRKSGGKHQMAACCACKKKRKKCDGKYPRCSNCLRLGHECTIIYPPTGREIQRDYLERLETNVRELEEKLQSGDWHKSSSEACLSSSASESPNEEPPYPARYPPTANKSKDLTQEVGFISLGAAGEPCYIGETSAYSIAKVISSSINCFPLSVNTFMDKTNASPIFELPFKFPSLETASDLLVCYKKSVHCQYPFLDWPFIENCFDLVVKKKSHGHVPIFFIFMIFAISSQIQDASNSNAYSSYTKSYYMKAFECVGLVIESVNIYTVQAFLLMSVFAQKMPDGVPGWQASGLAIRTAVVLGLHRKSYHRKNQPVTNEQRLLQDLKSRIFWSAYGIERINGLILGRPFSISDIDIDAPFPLETPETRVGCYVVKLRRIQSSICTFIYKPIPLVDQQEDVDATRVEILLELNEWLSTFPYKDNAVSTFETNNWSLISYHNSVLLLWRPIILEVAKLKEESPKRHLEWFKVFTESASAVCINYKNMHLKRKLSYTWSAMHCCFVSGISFLFCLWLDRSLKVLKWKRKSLVYETISACQTILYVLAERWGSASIFRDSFERLSTIVKTCIDSENDMEPHNTMNDILTSGIFVDGSIGIDSYLTGKQFKQKFPTIATPATSAAAADTGREPMDSGSDNLDSLWEFLDTTGDKYLRDLFNEMEDSISMNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDA6</accession>
    <name>A0A1E3NDA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_37638</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015217.1">
        <property type="nucleotide sequence ID" value="XM_019163097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDA6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDA6"/>
    <dbReference type="GeneID" id="30179784"/>
    <dbReference type="OrthoDB" id="29646at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="67"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12968" checksum="16735FB69ACDE829" modified="2017-01-18" version="1">MSFQAQYDEYQQTLQSLQDHVVSLESQLYEHEIVVETLKGVPAERRAWRLDGGAVGSSSGALVETNAGEAQKKLEETIKGLKQLREKLEKEAAGVKKEFEEWRVKHNVKVVRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDB9</accession>
    <name>A0A1E3NDB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_60770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015227.1">
        <property type="nucleotide sequence ID" value="XM_019163459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDB9"/>
    <dbReference type="STRING" id="763406.A0A1E3NDB9"/>
    <dbReference type="GeneID" id="30180146"/>
    <dbReference type="OrthoDB" id="2011998at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034431">
        <property type="term" value="F:bis(5'-adenosyl)-hexaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034432">
        <property type="term" value="F:bis(5'-adenosyl)-pentaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008796">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052745">
        <property type="term" value="F:inositol phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052846">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052847">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052843">
        <property type="term" value="F:inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990174">
        <property type="term" value="F:phosphodiesterase decapping endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901911">
        <property type="term" value="P:adenosine 5'-(hexahydrogen pentaphosphate) catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901909">
        <property type="term" value="P:diadenosine hexaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901907">
        <property type="term" value="P:diadenosine pentaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071543">
        <property type="term" value="P:diphosphoinositol polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071545">
        <property type="term" value="P:inositol phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF0">
        <property type="entry name" value="DIPHOSPHOINOSITOL-POLYPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="5">
        <location>
            <begin position="26"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="197" mass="22661" checksum="541803F2088CF7A4" modified="2017-01-18" version="1">MSTLHVTKTAKPREGRAEQMYNARNHARLVAGGIVLDDALDKVLMISSSKHRDRWILPKGGIEYDEKENFAITALRETWEEAGAVGAVTKKLPVVEDARDPAERAQMEDIPLCEFHFYQMKLVELDTDWPEASNRLRRWCTYQEAKHELLKSKRPELLSALNDSDIVKDHVEILQYDEHSNTLLDTQLDNDNGYENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDG7</accession>
    <name>A0A1E3NDG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015275.1">
        <property type="nucleotide sequence ID" value="XM_019160101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDG7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDG7"/>
    <dbReference type="GeneID" id="30176788"/>
    <dbReference type="OrthoDB" id="3979508at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="189" mass="20617" checksum="9BD29F3A608A5A08" modified="2017-01-18" version="1">MVKIAIIYSSNNGHTARLAKAVLEGVNRVEGAEGKLITSEEALERTVLDDLSSNYSAIIFGTPTLMGSPSAKFKEFMEMTSGVFFKKLWKNKIAVNTLTDLAVFAAQHGMVWVPVTVTPTWNLSTQKEENSLNGCGFFLGVGAQANVDQDGNAAPAEWDLKTAIVYGERMSEFAKIYEPAYKALKAQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDH8</accession>
    <name>A0A1E3NDH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_18435</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015285.1">
        <property type="nucleotide sequence ID" value="XM_019161999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDH8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDH8"/>
    <dbReference type="GeneID" id="30178686"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="102"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="551" mass="61714" checksum="8385E5F85F8E3AAA" modified="2017-01-18" version="1">MSDATKEKFEVNVDSIHHTSNTDTIDGDDINDLWKLEEPDIHTRMFYAQRSIETQYDYVSQGSRLFKFWNDMIYRSLKIESLNDNGKEYPKWVNNNLSMRTAHWIVCVGLVTSEIMGAYLVPNSLSMLGYVPSNIMLVVCCFVTLLAGGVIWWVFLLFDSPEYPVKTFADLAYVVGGTPCKQAVIFLQLIATLLSSATILISAAECVIILRTDRMCWVGLMVLLSGVMMICSFMKTLSNIGKYCLVVSACNYVNLFVQLGFIGNSEPNWANAESILGIPAGTVATYAFAPGQSLMNKVVAVTNISFVFAGSIVFPEVISEMRRPWEFWKSMLVAQSIILVVYLVYGNYIYANQGQFSNSPAVFGVSNISALKGLAFMTFVTGFIQNIFYGHISCKIVYKNYMPLISKTIRFESKRGIIFWSLTVFIVWVIIFVIGAGVPQVSAVSAFTSSLTMIPLTYVIPFALHLFSLYRSANENYIEDFVPHQSNVGDTSVVEYLRRGYKKYPLLTIFYTTLCLSSLAFSGLGLWASVEYIKYIFDVTDATSFSCTSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDK6</accession>
    <name>A0A1E3NDK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="3">6.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pantoate-activating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pantothenate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_74808</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-pantoate + beta-alanine + ATP = (R)-pantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.1" evidence="3"/>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015313.1">
        <property type="nucleotide sequence ID" value="XM_019164878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDK6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDK6"/>
    <dbReference type="GeneID" id="30181565"/>
    <dbReference type="OrthoDB" id="2020436at2759"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004592">
        <property type="term" value="F:pantoate-beta-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00560">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000013">
        <property type="entry name" value="Pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.10">
        <property type="entry name" value="Pantoate-beta-alanine ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00158">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003721">
        <property type="entry name" value="Pantoate_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042176">
        <property type="entry name" value="Pantoate_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00018">
        <property type="entry name" value="panC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299">
        <property type="entry name" value="CYTIDYLATE KINASE/PANTOATE-BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299:SF1">
        <property type="entry name" value="PANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02569">
        <property type="entry name" value="Pantoate_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="320" mass="35545" checksum="EC1D309C9D83F79D" modified="2017-01-18" version="1">MTMADIPIITTVADLRVLRHQYARRNESVGFIPTMGYLHEGHLSLINQSLLSNDHTIVSIFVNPSQFAPGEDLDTYPRDLDHDVKVITEFVAAKFQGSSDKKVDAIFHPTVLQMYPSGFSMERSQQRGAFVEVLGVSEPLEGKTRPSFFRGVSTVVAKLLNATNPTVAYFGQKDIQQTVVLKTMVRDLLMDIELEIVPTVRNSSGLALSSRNAYLSPEILSQVTCIFRSMNTAYGKYSKSGVRDVSDLTETVSAEIRSTNANFKVDYISFNDPETLDYLTTIDPKKGAILSLAVYVPNSTETNETKTTRLIDNMIFNPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDL4</accession>
    <name>A0A1E3NDL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_179368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015317.1">
        <property type="nucleotide sequence ID" value="XM_019161814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDL4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDL4"/>
    <dbReference type="GeneID" id="30178501"/>
    <dbReference type="OrthoDB" id="435593at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061015">
        <property type="term" value="P:snRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035719">
        <property type="term" value="P:tRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057941">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058537">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF53">
        <property type="entry name" value="MRNA TRANSPORT REGULATOR MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24138">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24139">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="1">
        <location>
            <begin position="26"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="940" mass="110134" checksum="A1424A76391BA447" modified="2017-01-18" version="1">MESVQKIEEALTTLYGNTDPGLKAKANTYLLEFQRSQQAWDLLFPILENQASSFELRLFICQTLRSKIQYDYGQLPDLSTVESLRLSILKVLLHLSNFKTDKLLVIQLSIALSYLIIQDLTWEKPILDIMNYLANNPVNLLEFLKILPEEMSDIKKLPLTNEEFELQYKKLLVDNVENIYYVLIQSTNSLTPENKDLETLILACIKSWINEIPIHMILNDNSALWNLIVVGFKDHETFDTSVDCMITIVSQIDIFDQLNENLPYIEILYKQLVELRPLIAENWDDPMVIERFTELYSTAGEAWHTLVVKDPENFQILVEIILQLTTYTEDLDIVKYTFKFWYELKSMLVLENFKSSKVVFKPIFINLTQILINHLKYPTISDSTNTSDLFNNNKENEDKFKDFRYEIGDVLKDCCLVVGQYDSLRLPFDGLKALIEDKSASPKWQDIECLLFSIRAMAKEIDKSENKILPQIMNYLVQLPENPKIRYAATLVLGRYTLWTNEHPEFLQLELNYIIDGFKLENSHYNHDEKTQIIIATSHALKYFCMDCNNLLIDYLEPLYNLYSNIENFLDFQSLYDIVEGLSYVLKKYIEKNILTNEAQCLSIIQMFWSSTIQKLNEFNPNYAISTFVMTEVLPMIFAVIQKYGKSSKVSERCIKFIRKCIQNFRRFMIPSLKSIEGLLVEGFQNYKFGCYLWCSGALIKEFSEDDDDIDEFIKIDPSTMGDVWVFAQEQIKTFIKIYRDENDDVNEDIVEDFYRMMNDILMFKPIELLNSFEIVEVLYSVSLEIVDKFNEFGILNLVVNYLTDLLSWSLENPPISIYYDIPNVLKYKIYGLLNANNTELVVKFLNYSILKFNEDLVYSAIELVIETFKINNCYKNQFENLNTVNAFLASLPPDIITDSEKMKFYTNLEVSLGSKNFRKIRSTLLDFIHWYKRKIVNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDL7</accession>
    <name>A0A1E3NDL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="5 12">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_37615</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="5 12"/>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015330.1">
        <property type="nucleotide sequence ID" value="XM_019163094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDL7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDL7"/>
    <dbReference type="GeneID" id="30179781"/>
    <dbReference type="OrthoDB" id="10067394at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000090">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="14">
        <location>
            <begin position="40"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="13">
        <location>
            <begin position="121"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="297" mass="32970" checksum="49C95E2BD54077B6" modified="2017-01-18" version="1">MPDYSHLLPVSTHWKQQITDFLSEDIPSFDYGGYVVGSDEKNASLYMKASGVICGIPFLEEVYGQCKVKVEWLREEGEFVSPLEEDPINKKIVVAKLLGPSKNILQAERVSLNILSRLSGVATRTREIVELAAKAEYKGIIAGTRKTTPGLRIMEKYAMLVGGADMHRFDLSSMIMLKDNHIWSTGSITNAIRNAKKVGGFAIKVEIEVQSYEEAEEAINAGGDIIMLDNFTGVELQSVARDLKKEHLGERQFLLECSGGLTKENLSEYLCNDIDIYSTSSIHQGTPVVDFSLKIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDV7</accession>
    <name>A0A1E3NDV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HDA1 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015422.1">
        <property type="nucleotide sequence ID" value="XM_019164805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDV7"/>
    <dbReference type="GeneID" id="30181492"/>
    <dbReference type="OrthoDB" id="3647690at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070823">
        <property type="term" value="C:HDA1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038609">
        <property type="entry name" value="HDA1_su2/3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021006">
        <property type="entry name" value="Hda2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026216">
        <property type="entry name" value="HDA3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11496">
        <property type="entry name" value="HDA2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02093">
        <property type="entry name" value="HDA1SUBUNIT3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="489"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="561"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="682" mass="78849" checksum="C3E7379F18B43DE6" modified="2017-01-18" version="1">MDLLRILDAKPEPCIADTDHFKVDPKYQFSNVYKIATPMSDFQKELIDQIVSLHYSDILRFFEKLDSESWDNMSSNRKEIITSSLETLLINTKLVCLHPYLLIDHFFPKSLTTRDLPNRLAETSGKFRILNDLLTQLDTIYNPADKADKSKKGKRIDIAVIVQPGKAMDLVDALCIGHKCNLNRYAGIKLRDSSANQKKNMHLNLNVHLIPSDFANINEKELATNLNNFTTKMNFVILFDLTVNLNDQFLSGITDVNKTKFFKLVPINSVEHIELYYEQQIQSRAIDEFIRPITAAIVVLRDRVGQLPSILKPAYTKNLLFLKQYLTDPINTKWPLPDLPSIGNFTSRDVERSLLTEVKFSFDNEEILKEEEENRPSVQDSLNTNFGGKTQMIGHIIQPRFAKKNAACKNYYDNKRLEKNYLMNPLNKDYENLTGISKEVKQHEVLTHTLIYHFDASIKKLIDLDKEVQSFNEFGDIRLKDFRQMVDAFTKLEESVKEKDNKLNDIDKDITEYGTKSENLKNDIQRYEELIESIGSSDTIDSNSKKLISKDIEKYTLLRDISKLEQKLETSTNESKYMEEEIERAEKSIKESKNAIEKQNAINEDFNVTIEEIKMEDTEVTREKQKLDLLTEKLKALEDQSTEVKQKMEDSLISLNEIGHRSRHVNYYGRNSGASLKKHALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDW4</accession>
    <name>A0A1E3NDW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015417.1">
        <property type="nucleotide sequence ID" value="XM_019164802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDW4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDW4"/>
    <dbReference type="GeneID" id="30181489"/>
    <dbReference type="OrthoDB" id="497927at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098:SF2">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="1">
        <location>
            <begin position="175"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="343" mass="38920" checksum="6BD3FADBC49672BC" modified="2017-01-18" version="1">MVDLDKHNVVPYFTSLGMFIIDEISFPTFKIDGILGGGGTFAICGSRMVLKHFESKKSAWIVDIGNDCPNEILEELKSWDSGTIFRYDYTRKCTRGWNGYGENEFRSFKYLTPKKRITVDDLKEYPWMLESNSFHFVCSPKRCVDLLREFSELSSTKIANPDPVIAWEPIPDCCTPEFLPETLSILNRVDILTPNAAECASFFGELEPQDKQNCERIARKFLDYMTKTNSGIVLRCGSLGSLLIESKHKFIKWFPPYHHKTSDSSNKVIDPTGCGNTFVGALATAFIKFNRNFEVACVYATIASGACIEQHGLPKLTKSDTNEELWNGISVDERLKNYKNKFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE38</accession>
    <name>A0A1E3NE38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18304</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015502.1">
        <property type="nucleotide sequence ID" value="XM_019161941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE38"/>
    <dbReference type="STRING" id="763406.A0A1E3NE38"/>
    <dbReference type="GeneID" id="30178628"/>
    <dbReference type="OrthoDB" id="2504561at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="8">
        <location>
            <begin position="291"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="805" mass="92361" checksum="43DA17AAE7246B54" modified="2017-01-18" version="1">MESSSKKLVLTPVITTSKIPILAIISGYCYKKIPQRCIPPILSILVEMIENEPDKNCSSGAEYDIVQKFFPSLSEIMELVGSSIRSFFNSSGVKEKETVERDIKSIHYYLLQQMWQLKSYDDFFTFLLSSANLLIDPSPQLIGSARGLPNINKPKKSLYCSSFLGSFISVIALGVTTLTFEEGIQIWKGFLLYRKETELHWITLNGVDSTLEFNNAKDYSTYSMFEKDILPNATIGINESTSLVYSHMDLASLFQSQVSTLQRKSSSLPKSLTELLSSLSQSDRSLIPSSYHVEHLNSWRQADYDKSFDSLHRYFDYMMSNRRQYFYHYALLALATLHSSFGANKEALRAIDEAILVARENKDLDCLNYLLTWLLNFIISKPQHFVKLKDHPSRSEIMDFLRLKTKETKNLSLQSISFQFEVVVSLLEGSSLTVVMENMVKTFYLILNFEDTDELKGIFITACQVADTIWKRIGYPSIGNLYLETAIDFAKEKNNEFDLILLYIRKANDLYFVGEIDESFQLLSSVKDSALKDLSMSKKWKMGHDIILFYHYLNQCKYPQSDILMDNMIALAEGLDDQEVTNELIYQRAIYNLKINNITEGIGIITKQLTSMKENSLVYNNHWFIRFQVLLSYTFTNYTPYPERGISILLSAANRAHACSLIFNLCECIICLCNLLLKVDPIQSIPDVKDLLSEFLPKILEIKRVDMIADAYHILSIVEFIEFEETRGTLTSDEFNTKVAIVLKYLSISMNGYEKMFDYVNLRKSLAFEKKVAHTCFLHDLVKKNASKLKELDLSLLKELNDPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE63</accession>
    <name>A0A1E3NE63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein FYV4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_13625</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015538.1">
        <property type="nucleotide sequence ID" value="XM_019160087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE63"/>
    <dbReference type="STRING" id="763406.A0A1E3NE63"/>
    <dbReference type="GeneID" id="30176774"/>
    <dbReference type="OrthoDB" id="18595at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01238">
        <property type="entry name" value="IGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="6">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="165" mass="18930" checksum="2CD816FB4DA23406" modified="2017-01-18" version="1">MLSRRLFSTSAALLKQSPLSKKIPQPTKEIPDVPAFLNRIGHNTAEYAEAFPTWDALFSSSSKEMKAAGIDVKPRRYILEQVENFRKAAFIENDPKAVFESVAEIKLHPKKNGGERNLNKYLAQKKVLQRIELAKTQKQHRKAQRATELKYKQLDKLHFQANQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEP6</accession>
    <name>A0A1E3NEP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DUF221-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_13276</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015714.1">
        <property type="nucleotide sequence ID" value="XM_019160037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEP6"/>
    <dbReference type="STRING" id="763406.A0A1E3NEP6"/>
    <dbReference type="GeneID" id="30176724"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF26">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G10920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="467"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="515"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="593"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="656"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="13"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="183"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="379"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="10">
        <location>
            <begin position="781"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="886"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="886"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="904"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="914"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="929"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="938" mass="105934" checksum="36A540C05FA81B32" modified="2017-01-18" version="1">MSSTATSTSTQSVITAVIANGVVCAVFIVGFFALRMKFTRVYDPKACTDFLPADQRPERLPRTPLFWLRALVAKKPAFIIKHCGIDGYLFIRYLFIITTFGLGGICLWVILLPVNATNGKGETGLNQLGISNVGSVGRYYAHVFMSWIYYSCVMFTIYRELYFYTNLRYLLMATPFYAKKLSSRVVVFQTVPDQYLDEHEFYKLFGGVRRIWVAKVSKKLSKKVQERAGLVANLEIQLNKLLAKAVKKKMKADKKGEPIEPANELVCYIPQKKMPTMRLTKYIGKKVDLLEYCKERLPELNIEIAKLQESENNVKIKPLNSIAVEFESQYYAQLAYQTVVHDQPLHFQPKHIGVAPEDIFWPNMRMFWWEQLGRLSGSVALVIFVIIIWAIPVAFVGFISNLTYLTNKMKWLRFIYHLPKVLLGLITSLLPTILLAVLMMLLPIFIRSMAKLSGCLTTQAIEYYTQNAYFAFQVVQTFLVVTIASSVSAVATQIVENPTSALSLLSADLPKSSNFFISYIILNGFAVSGGTLFQIVTLVLFYVMSFALDNTVRKKYDRYTTLSTYAWGTTYPIYSCLAVISLVYAIISPIISLFTFVAFGLLTMTYMYTLNYIVGIGSDSVGKNYPRAMFHLMVGIYLGEVCLIGLFVVSKSWGCIVLEAIFLAATVFFHVQMNIAFDNLTNVLPNTCMRPLDGKSETLSWKDPRLIPRYDDQYWIDKANKDTGGADGDTFQENVADSPFNDHYALHDHRTATDIQDVINEPLLIDGDRADLSKPNFFWRYLQPWKYLTYHDLQDYVPSSYYDYPDAQDDGDDGLRHAHDYDCPDLSATCPTVWIPRDPMGLSKVQIDDFRGIVPMSDDNAEFDSKGKIVFRGDPPVLSLDSYERHLESGRGEKGGRDSDCEDSGASASGSADADADAEEPGPAPDEDGSMRKNFERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEW6</accession>
    <name>A0A1E3NEW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_13352</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0612 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015800.1">
        <property type="nucleotide sequence ID" value="XM_019160049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEW6"/>
    <dbReference type="STRING" id="763406.A0A1E3NEW6"/>
    <dbReference type="GeneID" id="30176736"/>
    <dbReference type="OrthoDB" id="3992590at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013902">
        <property type="entry name" value="Mug135-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08593">
        <property type="entry name" value="Mug135_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="7">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="242"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="319"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="402"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="957"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="435"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="968"/>
            <end position="989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1067" mass="118386" checksum="52A7A37223613F64" modified="2017-01-18" version="1">MARPPNSWVWEHFRQDTSQPATSTHTSVECQICSKVLRYSSRNGPTNLARHLTRSHEMHPPDSHITRRPFESSFIKEQNTETNSGLIKKFNEAPGTVRASTSLQNLPADAFAEPHSSLQGAGEAAQSITATTTSATDASIENVSVTNSRPNGYHAEDERNISVLKVSASDSSLNSRSNFNTVNVSNVSNNPNISSTAIFHRPSASGGTAKISPGNETHGISNEGRVGTIDSGEISDEAISSEFPNNAGQIPENQSKRNSNLYDHNNARGETEAMNIVERLESANTAESEKDNRERPVKELSDKLADNRDPEYFKRAIFHSPNSENKVKRVHSRTHSRSLSDDLFSSNPYSTAGNITRSPNDLSSPSIVHNLKNNSRSEYENFSKNVNLFPIYRMEVGSKSKNQKNKLFPPSNAKAESSSTSDGNEIVSGVESPRLPSQQFSSPQVNSKQWKRNQHQQLSPTTLRLSNMTPGAITSFGSQITPKHYNQVDQQYFNNQQSYLFPVGNVEATSSNKLINSQLINNGGNAGNADDHIQNRNHVTNISMNAMFNSSINTFGNLDNTTVTNMDDIGSENVEPVPVLISGMRHNSAVVEGNNLPHKENGSKDNYETNSLFNKVRRLKVSNEGSPHSMKSSTFGIPSNVMIMQGLAGCEISPGHTVNRHQRQTSQQLKLRLQLQMQRQQQQQQIEQLNQLEQFEQLGQLNTMDQLNQINQLSQLNQLNQLSQLSQMNQMNQFSQFSQLNQMGQISQINQLNQMGQLNQMGQLNQMGQANQINQMGNYQQKFGAHKFIRSNPNPTDSLNDMPQMGTFSLSSENGLSSADAISSKRTGSTVQVSAVEHTDDDFNEGAEEREDGRVNNTYVDGKSTIAMLMEVIRVLQNSILRIEEDTMQQRNTIKHLQQKVARGINEKRGSGSRTPYDLLPWSGDGEKLPLLTDVLTLFSLSDAQLQAYLSLYEIEEEEEEKGSESSAKSESEHSDGKKANDTDHHEGVSESVGRIYGIDHSDDVAANEGASAELEITKKQEKGYNDYKQGNHKIGKPRANLFKLARFIGCIDVEKYWKVFSSGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF33</accession>
    <name>A0A1E3NF33_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_175774</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015847.1">
        <property type="nucleotide sequence ID" value="XM_019161653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF33"/>
    <dbReference type="STRING" id="763406.A0A1E3NF33"/>
    <dbReference type="GeneID" id="30178340"/>
    <dbReference type="OrthoDB" id="2192561at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007955">
        <property type="entry name" value="Bystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821:SF0">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05291">
        <property type="entry name" value="Bystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="499" mass="57976" checksum="C1C8D3E64C832467" modified="2017-01-18" version="1">MGKVSTKDIKDKSRHVPLYKDIASTGGKLKSSQIKSKVYKKPSEQSSKSQLGEEEGDGRDEYDLDAATSRRVLKLAKEQQQEIEDEENFNEVATKPRFQLIEHESEDEVEREEEEEEEDYEVEIVDEDDLNPSEIAMFETFFKAKQGNNIPFQSINLADKIMEKIAEASSNTQQMDDEEEGEEEEEEGRKPDGVYLPPRVIEAYQKVGESLRAWRHGKLPKLFKVLPTIKNWEDLLYVTAPEMWTPNAVYEATRLFVSNLTAAKAEKFVTLVLYPRFHQDIEENEDHKLNYHIYRALKKSLYKPAAFFKGFLFQLVEDQCSVREAMIAASVLKKTSVPVQHAAVALSWLLEQEFSPAATVFIRVLIEKRYALPYQTIDDLVFYFMRFRVITDNKSSFKIDNEADDVMDEANIDITQQRKLQEAPPLPLVWHKAFLAFAQRYKNDITDDQRDFLMEVIRQRGHREIGPEIRRELLAGKPKEFTLEPTQQKIDDDDIMSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF80</accession>
    <name>A0A1E3NF80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7 9">2.1.1.216</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_36856</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7 9"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015910.1">
        <property type="nucleotide sequence ID" value="XM_019163039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF80"/>
    <dbReference type="STRING" id="763406.A0A1E3NF80"/>
    <dbReference type="GeneID" id="30179726"/>
    <dbReference type="OrthoDB" id="6349953at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140691">
        <property type="term" value="F:RNA folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160103">
        <property type="term" value="F:tRNA (guanine(26)-N2/guanine(27)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000051">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 9" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="546" mass="60313" checksum="0F11D42D8B8BCD5D" modified="2017-01-18" version="1">MTSSKTSTPDIDLEKYDLLTEGSTTILFPKNNVFYNPVQQYNRDLSSLAIRAYTQLCLEEGALKRNKQPSGDAEEEAAEEPSSKPFARLLEALSASGLRAIRYSKEVPLLEHIVANDLSSHAVKSIQLNCDYNKTTNVKAQEANAITHMANSPSAYHIIDLDPYGTVAPFVDSAINAAKNGALLLITCTDLGVLAGNGYPEKCYSLYGGTNVWGDATHESALRLVHGMIARTAAKYGVSIEPVLSLSIDFYVRVFIRVWHKPIKVKELMAANEVVVKCSGCHSTTTQQLGKMTEPDAKGRRKYGLAKIQPGISSHCSFCEYTNHMCGPMWGGPLHNKKFIDRILKLVDEEEARKAPNETTYGTLKRVRGMLTMAKNELGDAESSESDIHDSQFYFATTTISRVLKIPAPSLEDFCAALGNLGYDASLTHAASNSIKTNASWNVLWYIGQQFAKRAAVDAQRLSHTTAGYKILTNETIAKSIDLRAVMKEKLALTDDDAAAKTDKDVLEWLFTPNSVSNHVQKLRRIKIVRYQENPTKNWGPKARPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFG6</accession>
    <name>A0A1E3NFG6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_59752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015994.1">
        <property type="nucleotide sequence ID" value="XM_019163387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFG6"/>
    <dbReference type="GeneID" id="30180074"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="58" mass="6027" checksum="87B7CCC8DD57CDF8" modified="2017-01-18" version="1">MPFHLGCSASPDACSPLLPSASASFNLPRRDLVTAKIILVPVKPADDNLYSATEAGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFI5</accession>
    <name>A0A1E3NFI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 11</fullName>
            <shortName evidence="6">U3 snoRNA-associated protein 11</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_60751</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Nucleus</location>
            <location evidence="2 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UTP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015206.1">
        <property type="nucleotide sequence ID" value="XM_019163456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFI5"/>
    <dbReference type="STRING" id="763406.A0A1E3NFI5"/>
    <dbReference type="GeneID" id="30180143"/>
    <dbReference type="OrthoDB" id="29058at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015952">
        <property type="entry name" value="U3snoRNP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="245" mass="29155" checksum="5E3920B4B546AC34" modified="2017-01-18" version="1">MGKLVHNVQKKQHRERGQITSRARFGLLEKKKDYKLRSENFHKNQAKLKLLKEKAKNYNEDEFYHAMKNRETNEDGILIQKRQFSENLSNDQVLLLKGQDLNYLNTISSKENRKIEKSMNSMNLFGSNGKHTVFVQDKEEFENFNPEEYFETDKALLGRRENRLKTSQLLSSNKSLSQDTESLEESLAYKRRELDVLKQRIEREKKLKDVKSKLQLQRELMKPGSKKKITKDGQVVYKWKNERKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFQ1</accession>
    <name>A0A1E3NFQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Threonine dehydratase</fullName>
            <ecNumber evidence="14">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_36017</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="14"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016088.1">
        <property type="nucleotide sequence ID" value="XM_019162979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFQ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NFQ1"/>
    <dbReference type="GeneID" id="30179666"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04907">
        <property type="entry name" value="ACT_ThrD-I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1020.10:FF:000001">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000008">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000005">
        <property type="entry name" value="Threonine dehydratase catabolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="entry name" value="Biosynthetic Threonine Deaminase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006674">
        <property type="entry name" value="PRK09224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF11">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 14" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="15">
        <location>
            <begin position="444"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ44975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="525" mass="58466" checksum="6A852DA4B9F6D27F" modified="2017-01-18" version="1">MFPSLKPADLLPDGTPDYFKLILTSRVYDVIDETPANYAPLLSNKTNSHVFLKREDLLPVFSFKLRGAYNMIANLSPELRSKGVIACSAGNHAQGVAYSAQKLGISATIVMPVGSPSIKFQNVSRMGSRVVLYGEDFDAAKAECDRLAHEHGYTNVPPFDHPLVIAGQGTIAIELLRQIKSNKLSAVFVAIGGGGLIAGIGSYLKRLAPHVKVIGVETFDADDMYRSLKKGERVALNKVGLFADGTAVKLVGKETFRVCQKFGIDEIVRVSTDEISAAIKDVFEDTRTIMEPSGAMTVAGMKRYIQLNPDINHSDKTYVPVLSGANMNFDRLRFVSERAVLGEGKEVFLCVQIPDVPGTFRKLQNIIHPRVVTEFSCRMSDSDINAEFAEVYTSFAVIDREKELKQVLTAMNKEGFVAYDLSKNEMAKSHGRYLVGGKNSLREKLIHFEFPERPGALDKFLELLKTEWNLTLFHYRNQGDDIGKVLTGINVPEAEDDKFQEFLDKLGYKYTDETHNKVFELFLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFX2</accession>
    <name>A0A1E3NFX2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amine oxidase</fullName>
            <ecNumber evidence="4">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73839</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="4"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016158.1">
        <property type="nucleotide sequence ID" value="XM_019164604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFX2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFX2"/>
    <dbReference type="GeneID" id="30181291"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="475" mass="51391" checksum="DE5A53429070F12C" modified="2017-01-18" version="1">MTTDADVIVVGAGISGLKAAADLHENGVSTLILEARPRTGGRVYTERATSTGNHYDLGATWFHSTMENPIFDKFVGEWYTPSDARYDDHNVGFVLDTATGGFPDGCNVGPIVDEIKYFASKSPSDETLQNSLLSYFKSKKGLLKEDEKKYAAALYKFAELTGGCRWDMISSKLAYGPFTGRDSFNTLGYDKVLEKISANYPKDQIKLSTVVKSIEKVAAGDADKTVVKVTTKAGDIFTAKYVLVTIPLGVLKLSVEDPSAEGAITFQPELPKAITDNFSKTHFTTLAKVIVEFDEVFWPDNDKFLVLSVPQDGDIDESSVYSAAQLDDYSAAKPVKAFDFPCLVSNFNVVKGVPALMFLVPSQPAHQIESAENPEKYGFELIKPIIEKLSGGKPVPQPKLVLTTSWGSDPYSRGVIAANAPGDMLVNDALIDGFGSIRFAGEGPVYEGHTCAHGAYISGEREAKVILAKLGKTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG37</accession>
    <name>A0A1E3NG37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_21166</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016220.1">
        <property type="nucleotide sequence ID" value="XM_019162189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG37"/>
    <dbReference type="STRING" id="763406.A0A1E3NG37"/>
    <dbReference type="GeneID" id="30178876"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="67" mass="7304" checksum="3D206F1FE4A56DB7" modified="2017-01-18" version="1" fragment="single">CTNCHTKTTPLWRRNPEGQPLCNACGLFLKLHGVVRPLSLKTDVIKKRQRGSGVTKKRRGTGTGSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGB3</accession>
    <name>A0A1E3NGB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP-2 complex subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_59972</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016284.1">
        <property type="nucleotide sequence ID" value="XM_019163405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGB3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGB3"/>
    <dbReference type="GeneID" id="30180092"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="995" mass="112361" checksum="1E685D81FD840546" modified="2017-01-18" version="1">MSMNGLKQFITTVRQSKDSSDESNKIDKELKHIYEQFHNCAKVSSSTRLSNYHRRKYICKLMYIQLSGYDILPADKKDKDGLVRLGVEQCLILLNAQLSNYTDDSNKDEGVVEDIDLNLKELKNDRDIGFLGLKSLYNEHITVHDLESIVNSVLIDISSTIVLKNEKNKNVMESLWIIVGIGLQSVSELIIWVKNLSITEPFMSEFVSSVFELIHLESMKVPEFVSTRAMLCIAQLFKLNHTGDLKKEVLTWDNLAFVGKTLSQSNLNDTSIIISLCTLLEKWVVFDWEGIKSLEGLLITKLTEVSEILNSIEFENTKNNTQNSDWILQYGYMIISLFNVLSHITTVAHGTGVATRPDLKNTVDKIISNCVNTKAKLLTDNATNNAQNQLIVASVLFAALDLYINDVNDAGNLDRSDVSTVMEALVTFLQFNNDVNVKISCLKKLILVMEKTSDYGFGRKILLVESAMWRKFMHDKDLFVCKLTARVLILATDRNIRASDDIALNQMKDVLALLMYYLQKCENTERGIVLSDILTILDDYKETGIIQDVSRKILKIFVLIGNCDDEAWAKIYELIIFSTTTSDRKKLDLAQVAIDFIVKSLSIGCCENFIKLSCLVFRHNCTYWNLGDMDAQLSLLIDKYENSTLFTKLLILDTMDAYYDIVKDAKLKGIIKLAFQQEMQSNNIEIKQRAHEYFTIIKLDLSLNKKISLSNFETLPPLATSDSSLLPLPLTSPSLPALSEKWEEGYIRLLRFDQGILYDSANIRITFRVKRDKKFSQIDFNYKLKIKNTEGFGSKIVEANGSQDFYSIEVAESKPGLVNESENGTITVRFSIKRMYPENKEPVLQFRIPSAGEEINLRLSLAMKLLHISEGNPMSIQTFQSRWNQIGQFMPEKGCYKLDISTTTSSTTSDNVTHIVELITRCMKKMNFEVVSAEGGTNVGQEGFVVTCASILTLEDHPVGCLVVLTASSVEVRCTDVGIVEGMCERIAAVLGNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGL5</accession>
    <name>A0A1E3NGL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015596.1">
        <property type="nucleotide sequence ID" value="XM_019161978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGL5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGL5"/>
    <dbReference type="GeneID" id="30178665"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102:SF2">
        <property type="entry name" value="GAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102">
        <property type="entry name" value="SLR1143 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="64044" checksum="401EAA15D0D6A712" modified="2017-01-18" version="1">MRFEEYSPARRFRMEGYERQNNFNRPKASLVNNGNTAVNKVLLENNLISNKDYDLFPSPFTKEMFLEYYALGKQNLSTIEFPPSLEGTTFLKAPESYYEIRRLNTVEKFMNLPHWEHSNRFNVLLKKMMSMFNCKGAALSLIDSRMQIIKFQHGLGFDQCSRQVSLDSHAILSPGYFLLLDASKDWRFKTNPIVKSLPNIKFYLGVPLIASNKQVIGVLSIFDSFARCNNDESTVKIMKKMSSEIMEYLETPIKKRNHDHRDGTSRMLQSTCLPTGSNSRSGDQNSNNSKLLEIYGRATSNNKRNEEIIFEKDGSGTSYKYHSSLKFSKYSCPYDDLIDLNVWKKLSRCDNLRKASNLLCELLMNKLSLDCIYICNVKVTKNCWINKQFYPLNEREIEVENYKFEDKIEWADEENEYENEVMNDFGNMKMKVIGMAARETRVENMLDSDNAYEFHSKVIRSVNGLNYQSADSRVIFHSGYSLPFYRHPNKLVRKSRMKALQSDRNEIVELFFKSNGYLITCFDSNGRDISESEIGYVYGCASILRRIFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGP4</accession>
    <name>A0A1E3NGP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LCCL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015851.1">
        <property type="nucleotide sequence ID" value="XM_019160054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGP4"/>
    <dbReference type="GeneID" id="30176741"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051957">
        <property type="entry name" value="CRISP-LCCL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331:SF1">
        <property type="entry name" value="CYSTEINE RICH SECRETORY PROTEIN LCCL DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331">
        <property type="entry name" value="LCCL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G08630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="712"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="758" mass="80828" checksum="596BDEFF1E140EBF" modified="2017-01-18" version="1">MEHTAEHTVERSRSFRSDNSDGGDGGDGADSVEGVVRPREWVHSAESKYGEDGERQGDDIELATYRVGSGQSDSGSDGVCGGYHDDEGAGESDSDSDSDNGNGNGNGNESERHAAGSVVWRRLLRIWRGPSRPSDRIEDFRHGYSPWVYLVNRVPLALGQRAGRRTRLAVLAVYFAGLLVTLRLLYGHLLFGAPAVDGTPAVTFPCGAAPKLWLGANDKCGLHARRCMGPFRRHETLYVRCPAFCGGAGLAYDVVKYADRRVQYQPYIVQAPAPADDGSPVYRGDSFPCIAAYEQGAISQLFGGVVALHLDDHFPYDTAEPPRTNDSGPALLFDSSFPASFTIGPAVGAVRNGHDLRLLGIVAGLVFTAAAALLAYDARVFYLTTVAVLYTTIVLCSDPPVIVDFLDNRYGGGDSAWRLLSLYVRRLIPLGLAVGFVWKCVGEYTFRAGGAGGVQRLLFLAGAWLTALNNLTFDRLPLDRLLPSDIAHRPSSIAAFLFLVGLLLCCAVVQGRRIWRAGWFRRAAVGYAAGLAGLVWVGRGCSGLLALDGVPGGDVPGLVLRVHHWALGLALVFACKTRWTGSYIMQGLCVGLLLNGVGRWGFASVLEQHGAVLRDRDSVAGHLSRPAGVRFDPATRWAQVLFAPADSAKITVSAGPTPAEQTVSVNGRRARVKLLVNDVLVYAGPARGVLVPAPAPAAPLYLRAAVCYRPGPPAGPDSGLDGAGDDDDDDDDDDSAADGSAASDRGFRCSGWSAAMRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGS1</accession>
    <name>A0A1E3NGS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016468.1">
        <property type="nucleotide sequence ID" value="XM_019161760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGS1"/>
    <dbReference type="GeneID" id="30178447"/>
    <dbReference type="OrthoDB" id="3357002at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021460">
        <property type="entry name" value="DUF3112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35184:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35184">
        <property type="entry name" value="YALI0C10208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11309">
        <property type="entry name" value="DUF3112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="431" mass="49395" checksum="E9F111E2F22F649C" modified="2017-01-18" version="1">MSAITNLIKMETDNAADGTGIVKLVDLTKKLLHLDSAKKIPTSLINYVQGQQQSQFGSYPDHKDVAPSAVFLAIFVLIAIAHGSLFTINYMRGHKFWLSFAFCFYATLRWLGFALRIVWAKDILRLHVGIASEVLLVLPIVFLASFNLVLAQRIFTWRHPVFGNNNYFWYLMIALYSVVIAVVIMTIVAGVVPYLYFLSRSHYDMCRNVVKVTSILITLYSLLSLAFVLFTYLLPVSKANREALVYQPFWIKSFSPFYFPKKNAAQEGETVFLERYRNDPRTPKRTIVGGGMDIIDNEDKTEVEELREYEAVGEQKFSLKHNISICIILITSIFVFIGSLFRCIACFMDMVFLNQSWIYDPVVMYVLWGALETICNVLYLVGRVDLRFYRPDRLSKKQRELYTSNFPATNDAKDFGESTNLSSRQSSDTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGU3</accession>
    <name>A0A1E3NGU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 58</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_176898</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for pre-18S rRNA processing. May bind microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015901.1">
        <property type="nucleotide sequence ID" value="XM_019161697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGU3"/>
    <dbReference type="STRING" id="763406.A0A1E3NGU3"/>
    <dbReference type="GeneID" id="30178384"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902570">
        <property type="term" value="P:protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000003">
        <property type="entry name" value="Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="283"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="401" mass="44136" checksum="717E5DF7DF502A6E" modified="2017-01-18" version="1">MSYVLTETAAGYALLKASDKKIYKSTTLQTDLDSAEKVLSQFKIKAFSKFASAANALEEANLVNEGKVSDQLKKLLEEAKPDKKTTLIVDDTKLANSINKLGLSYSVVSDAASLDIYRAVREYLPELLPGMTDKDLSTMSLGLAHSIGRFKLKFSPDKVDVMIIQAIALLDDLDKELNTYAMRCKEWYGWHFPELAKIVVDSVAYAKIINKMGIRSNCEETDFSEILPEEIEQQVKAAAEVSMGTEITEDDLLNIRMLADQIIEFSQYREQLSNYLSARMKAIAPNLTTLVGELVGARLIAHAGSLISLAKAPASTIQILGAEKALFRALKTKHDTPKYGLIYHASLVGQATGKNKGKIARVLAAKAAVALRYDALAEERDDSGDYGLEVRSKNPKRHNHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGZ9</accession>
    <name>A0A1E3NGZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016469.1">
        <property type="nucleotide sequence ID" value="XM_019161761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGZ9"/>
    <dbReference type="GeneID" id="30178448"/>
    <dbReference type="OrthoDB" id="10262413at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051604">
        <property type="entry name" value="Ergot_Alk_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43162">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43162:SF1">
        <property type="entry name" value="PRESTALK A DIFFERENTIATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="292" mass="31892" checksum="898B8FA23C999FFF" modified="2017-01-18" version="1">MKIVLLGSRGNIANPLSKLLVSKGADVTVITRSQKNVDAIESDGAKAAVGSVDDVEFLTKTFDKATSVFILIPANLFEKNALKTMIAQCNKVCDAVLKAGVKHVVYLSGIGSDIPEVGFHSENELILKNKLAGLDSVYLVRPSDYFANFLRNIDSIKKDGAIYSNTPKEQVESYVHPIDVAEVCSGLLFSPGNDMFKIINVEGDFASGEQVEKLLMDAIHIPVKWVHLSDADASKALVSAGMPEENVMPLLTIFSETYHNGITNFIKNQGSVYGKHKLADYFKKEFAAEYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH17</accession>
    <name>A0A1E3NH17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17879</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016522.1">
        <property type="nucleotide sequence ID" value="XM_019161790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH17"/>
    <dbReference type="STRING" id="763406.A0A1E3NH17"/>
    <dbReference type="GeneID" id="30178477"/>
    <dbReference type="OrthoDB" id="10263185at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="378"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="591" mass="68553" checksum="3648372162FE9B31" modified="2017-01-18" version="1">MAAKKGTPQMVTKKAKSTSQKGKIPEKQVKVPSKASTKEKRSASKSTKQESKNEIGTLEEFELIRKSIEESPKNYNKLVRLLKSLHMVVSQINDENQREMASNFMNIARGLIYYLYKIFSHLISKRLLKVFKGISEQEETVRKWLISKYESFQKELFDLWSIEHFTESIATLKIDALENLMNLIQDEAKYLGPSIDEAYFPTSRYRKILQMLLLSGNELSINEEDSCANDYLLLEFKETYFEKYWDLKFYFFSELPHIEEDLRESEIGHRLVFSKLVALTKSAPVYDVENKEGITEIPVWVKNAPQNTMYSVNTFRNNFEKTWIGALNLQHLGKKEYSSALAILHKRIIPFFGDAQKLMDFLTASYTLGIKTKDGLLSILALNGLWELMKSYNLDYPNFYTNLYAILTPELLHLNEKSRFFRLLDLFMSSTHLPASIVASFIKRLARLCLTAPPSSIICVIPFIYNLIRKHGHSTCMLLIHSTTTAEEDADYVDPFDENETDPSKTEALGSSIWELEAMINHYHPNVSALVKIFTQHFTKYSYNMEDFLDWSYAKLIDSEMKKRFKGDLGVEFEKWNGMFGDEGYMSDYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH27</accession>
    <name>A0A1E3NH27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_74179</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016532.1">
        <property type="nucleotide sequence ID" value="XM_019164698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH27"/>
    <dbReference type="STRING" id="763406.A0A1E3NH27"/>
    <dbReference type="GeneID" id="30181385"/>
    <dbReference type="OrthoDB" id="3361892at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032931">
        <property type="term" value="F:histone H3K56 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016849">
        <property type="entry name" value="Rtt109"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF2">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE RTT109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51728">
        <property type="entry name" value="RTT109_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="456" mass="51388" checksum="96F484F86BEEA00A" modified="2017-01-18" version="1">MTVQHSIDDDGAVRQRAGGLFDRLAPHLKSPGKYVLFNVFSTKPCVPVVHKPKPLRPLLVTRTTHYCVLFSPDNVPLVAVEIFTYCTFYKDTINKLVYVSKADTTGLAGSADVNVGAFVTEYLKWVCQMPVEDLVKGVKLKKGQKQRDADSAQHGAENGDVPGRMFVSETAHALYVLQRRASGDIDYRMPASSRKIDALEYLAALGIEPAALANENVQTRLVLFTRSEGQYLFPESMRNPDKHVLDGRALLKWWLKNVDRIADDWTGPAKYLNILNSESRDIMRYFPSSSWKIGNVYDSHGNTNEPAIYAIPLLPDDPKGRFLEHLVVEGRAKKVEGKQFWQELAIRQEFSFGAVVGLIGISGLPSYNLPNCDQNEYNLLSPTSLNHFKELVTTKDYSDRSDWGTLLEELLLTPKLKQYDIHGRWEGRQSAKRKAETVKPVINTLMCVRSKKKQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH99</accession>
    <name>A0A1E3NH99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_16830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016638.1">
        <property type="nucleotide sequence ID" value="XM_019161352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH99"/>
    <dbReference type="GeneID" id="30178039"/>
    <dbReference type="OrthoDB" id="3997906at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="410" mass="44420" checksum="5AD9F5ADD5FB0BD3" modified="2017-01-18" version="1">MEGEGTGTSCGRFHRYGVSFEKSHSASSLSMISNAAPREDNGIGGPGHGQVPGPGQQLVGSCSLSSSSSSSETEAEPRLSTPANPGGASDASRMKSSFINVFSLKSKIGTDAGGTRAVGDPLVGSAQVIPPVVLPPKETSSPNIFKKIISRKALIPRMKAFKRIANDMQMESYPLDDEMQHEMIITTAMKEDDELSNSRSSYSFLLSQKNSPRNILNQDNLKKFEVINKANESWNNNRRKSSSSLTNSESYKSNSRQGSMSNASINLHKRKTSVTSLIPTGTNISASTTVADASTAREEGGDTPYHLLHGTFDTSSHSLLSNPFETIRKSRKRKAKAYDDCYTDVEEYLSDDGGVSPWNPMMNCKRRLVSGSVTNSPKSPALDAYPSRRNSSMMQSLQSASDDFEQMSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHS7</accession>
    <name>A0A1E3NHS7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein YTP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_178737</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016805.1">
        <property type="nucleotide sequence ID" value="XM_019161787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHS7"/>
    <dbReference type="STRING" id="763406.A0A1E3NHS7"/>
    <dbReference type="GeneID" id="30178474"/>
    <dbReference type="OrthoDB" id="4005299at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018825">
        <property type="entry name" value="DUF2427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10348">
        <property type="entry name" value="DUF2427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133380" description="Protein YTP1-like C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2427" evidence="3">
        <location>
            <begin position="74"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="4">
        <location>
            <begin position="309"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="73008" checksum="DFC8FADCE5AB1D9C" modified="2017-01-18" version="1">MKLNRLTLTALLLAFSGTVLSNSEPHAHNNIITMGTTTADWDMVKATPWAGGHVHGVPILNTTLTLGQQKYWKSYNTTTYLTLDSPYSPYVHLHLVLLFVSAFVFYPFVLVLNNLESNWFLPLLAVQASLSVVSCILYSVFINNVPDLYPNMAYTKMVTGWFVLTLLQALFAVVNSVRRWLPSNNSISGFQPFPTTDSSHYTDDVVNNSSLSNEAASTSIQLEDFFTPSSNLLANSMEGLNSSFNSDSSLRIPSGLDTDFSSALTTKVKSYSSLLGQTISRILYTLAFSFGSLFTILHNVTTWALLAYFFVLFPTGLSCLSLLGKERRVFNLLAHLIKGGIFFILGLVSLARYCGCFAGIAGAWNYAYVDLDSNKSQESSTVSETDRVMSGMGGTGSGGSNVSHSVWIRFHQALNSYENRGGGLICSFEAIESFLIFSMHLGSRDGSWSAMDLQHVSIAFMYFGAGLCGLVTELKLSSWRRSLFFNVAGKQLEIAENIKLDDDLTADEKLRIIGSKKMITPGFSPNPFPVFTIFWTGVLMSKHAQASALSSAIHVQWGSLLTYGSFFRVCTFLLMSYYPLHGSKECFLPSRPFTELVTSFCLLCGGMVFMESTDQVVEALAYRGLTPMFTLNVSVGIASLIMAWVMLIFAVKDRMRAEQLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHU6</accession>
    <name>A0A1E3NHU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase inhibitor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_35163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016807.1">
        <property type="nucleotide sequence ID" value="XM_019162926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHU6"/>
    <dbReference type="STRING" id="763406.A0A1E3NHU6"/>
    <dbReference type="GeneID" id="30179613"/>
    <dbReference type="OrthoDB" id="3996456at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24088" checksum="FE5C4EC6A5F7DC65" modified="2017-01-18" version="1">MPKGILKNAHTDAAYRTESPNELDREKAVFDRQKVIENTRLNAQLSHAKGVPGLQHIEQKKQRGEALSEEEQLRWDERNLLVNEMEKSATMKIDEPKTPYEGGFDPNNDYYRTDNEADDGADGADARDDPFDLGEGLDDADDVQRGHIDVVAGDPPHPVLAAAGPAEPSADEKHRRFEEKRKQHYHLKAAPLKEHHVLPAADDDDDDDDTAADRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI74</accession>
    <name>A0A1E3NI74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_25750</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016899.1">
        <property type="nucleotide sequence ID" value="XM_019162411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI74"/>
    <dbReference type="STRING" id="763406.A0A1E3NI74"/>
    <dbReference type="GeneID" id="30179098"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="241" mass="27300" checksum="64A47902AD2CC598" modified="2017-01-18" version="1" fragment="single">INKHKYKLFEQIGKGGSSKVFRASVIDSPHRYLAIKIVNLEDQELSTVSELKGEIKILHKLRHCPRVVRLLDYSLTSKVIFFVMECGDLDLATVLSNRHLMSRFYDLEFIRYHAQEMVKCINAIHEMDVVHLDLKPANFVFVSGVLKLIDFGISNSIKGHTVNVYREYQMGTPNYMAPETLLDCAESDNGSSVWKVGKPADIWSLGCILYQLTYGLTPYASYTGTKKILAITNSSINISYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIJ5</accession>
    <name>A0A1E3NIJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MATE efflux family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_12398</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017070.1">
        <property type="nucleotide sequence ID" value="XM_019159922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIJ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NIJ5"/>
    <dbReference type="GeneID" id="30176609"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="534"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="571"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="595"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="70872" checksum="7D7F69EC4912B9C3" modified="2017-01-18" version="1">MSDGDPLTPQKNTQRRRPTGLFSKTIGQLPRSLLTSNNVAINSKRRKIPLTFLPPSTKSPLFVYSNDYDQRSFLSLCSGSHIDEADSFHDNLRGDNESVSTFRSLPSTLEGNNDEIESIHQWLDEERERRSLVSGEDLGETYGAINEGSDDIMARADRYFPSNTRRSSFLSLEGIVKDEEEEDFEFEKVTTRKEIDKLFRYSIPLIITFFLEQIFSLVCVVFVGHLGKEELAAVSMASMTSTIVLAIFEGIATSLDTLCPQAYGAGQYKYVGIHTQRCSLFSLVMFVPAALFWYFSGYFLSFIIDDRKVIKLTQQFLRILILAGPPYILFENGKRFLQAQGIFDAGTYILFITAPMNIFLNWLLVYSETFGLGYVGAPIASVINFWAMLIFMILYIVLVDGSECWDGFTLDAFNHWYDLSKLAFPGIVMLLAESMAYEVLTLFASKFGTSALATQSALSSVVSLLYMIPFAIAVASSTRIANFVGAANIQGGRIAINVGFISSVLIALINSAVIFFGARHIALLFTDDEEVITMFVNLCPLISVFVIFDSLACVASGILRALAMQMIGGTISLVGYYIIAVPLALYLSFHSNLQLLGLWLGNGAGLLTIGITEIWIIYNVEWKEVIEHAKSLALHGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIR8</accession>
    <name>A0A1E3NIR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73504</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017142.1">
        <property type="nucleotide sequence ID" value="XM_019164502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIR8"/>
    <dbReference type="GeneID" id="30181189"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060567">
        <property type="term" value="P:negative regulation of termination of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000001">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="7">
        <location>
            <begin position="164"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="350" mass="40333" checksum="52DC1B4F9C4183B9" modified="2017-01-18" version="1">MGSEQPETYATDKKTLPFSEQHYFSSYDHFGIHEEMLKDRVRTLSYRNAIMKNKHLFKDKIVLDVGCGTGVLSMFAAQAGAKHVLAVDMSNIIEMAQKVIDVNGFSERITLFRGKLEDVKLPYDKVDIIISEWMGYFLLYESMLDTVLVARDMYLKEGGLLFPDKASIHVAMIEDEEYKNDKIHFWEDPMQLYGFDYSPFVKIAMVEPLVDTVDNGAVVTSHYKLIDFDLNTVTIEQLAFHRTFRLKATRDDRVHALLAWFDIDFPSDTPENVVSFSTGAHATYTHWKQTVFYLDRVLDVKKGEEISGSLASRPNEFNNRDLDIELEFNFPASGPDDSRRTTGKYNYFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIX4</accession>
    <name>A0A1E3NIX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017173.1">
        <property type="nucleotide sequence ID" value="XM_019164508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIX4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIX4"/>
    <dbReference type="GeneID" id="30181195"/>
    <dbReference type="OrthoDB" id="343092at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="779" mass="87903" checksum="21E76D4AB3BC9621" modified="2017-01-18" version="1">MSQDLVVLDSNDRRNTVGIGESQTSFALISDVDTDGDTDMDVVCSSSKQNRGSREGGTQRSKSGGSQGLDDEEFLEIFGLPRTSIRSSGSDTQQTRKDSGSSSWSSSSFGGSLQSQREKGEVIEIIDDPADVPAQDSVPGRLNFLDSELICIEDDDEDEAQVVEEAARVYPTSSPNTQLNRSIDLIDIDDHEEPEEVVQENHVHIHSGSNTNTSTVIDDSLSVIMNAGSVPLPFPYETPKRNRVSSSRQRANIGSMSSPIFETAGRRNAAERNLPKSKISKPAALSQKQKPSGVMQNVRHSILNVKDRDDRTTTASTKSPRSSVNVFNTGRPDRTLSSEVSEFSSSLNEIEYCNERLLTDEELKEKRRTMKSIKKRESNRVKSLISDLAGVRNKRVDSSSKYSKSVSSVLNFVKSAANAQSSETSEISNSSTLIKETDNNFMDNVTDEINLYTDERLELMLERAKQLEPEKLKKVNQAKYTKAELTEKITCCFTNVLNEKLELLNKDYTQHVQPAHFEMIEEALPIIRFKRYVDSALFKKKNTYVPIRPRHLFENFTILLYDSKDLIELFKKGIMKRHLNSVKAKYKETKIAVWVTGYDQYLQSLKTKANRAYKEKVQERLGSTADDSSRKKKVTQSYEEMIQPSHIKQKLLRYEVEHGFAFQSFKGLGDMLEWLKYIAYTLSSKYNDSLQRNGEVTNIGKVKSGETPKDCIVMMLSQIKGMTESRAKKFVDESSYKSVAALFDDIMSGKNITQSKTLRTDHERLIKKIFLSRNENDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ70</accession>
    <name>A0A1E3NJ70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_73630</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017273.1">
        <property type="nucleotide sequence ID" value="XM_019164538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ70"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ70"/>
    <dbReference type="GeneID" id="30181225"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180047">
        <property type="term" value="P:dolichol phosphate mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF0">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="251" mass="28956" checksum="45D047DE40F4F4A3" modified="2017-01-18" version="1">MSFADREQPKTICLFDVDGTLTPARLTVSPEMKQVLKDLRKKCAIGFVGGSDFAKQLEQLGPDVLQDFDYCFSENGLTAFKFGKPLPAQSFINWIGEEKYSELSRFILRYLADLDLPFRRGTFIEFRKGMINVSPVGRNCTTEERNEYEKYDVPNKVRVNMVNALKEKFSDLELTYSIGGQISFDVFPTGWDKTYCLRHLEDEKFDKIYFFGDKAYQGGNDWEIYSDDRTIGHAVTSPEDTIKQLKEIFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ91</accession>
    <name>A0A1E3NJ91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ribosomal subunit protein uS4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_16127</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017327.1">
        <property type="nucleotide sequence ID" value="XM_019161095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ91"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ91"/>
    <dbReference type="GeneID" id="30177782"/>
    <dbReference type="OrthoDB" id="3356781at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000025">
        <property type="entry name" value="30S ribosomal subunit S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="11">
        <location>
            <begin position="102"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="473" mass="54902" checksum="95868B969748F0C2" modified="2017-01-18" version="1">MTRRAQNLNSLVRGRIRPSMNKYNLFNLYKKSKPNFRIMTLYQQKWSSKQETRGYHGEHIKENRWQSFFEKKLEGVAQLDASLKGNVPSTPMVLQTFAPLEKRLDYAIFRALFASSVRQAKKFILNGHVTVNGVVIKHPSFSLTPGDIFSVDPERVLEALGAKKPSLKESYKIDKMQVIKWQSYVNEVQNDPRKVWEKKMEKQKKVNSLYKNYNVNGVLTENSFASTDAAERKKNMQDYKIKKMKEVQNSIDRKSILKDIIKTSPVESAAAFKEQFGDKLAAKAFQVYQELPSAIVKLQTDKELAAELEKIVPSYKDGKPVGDFYDDSNFKKIRSQLSELQTEYLEKIRQDFEEKPLAQDELIKIWSESLTKHPKLPPFNEVEEKGTYFVKLPWQRGMHGLADPSKSYFTPWKPRQFLPVFAVLPKHIEISFSTCHAVYLRDPVARPGESEVISPFDEDIHERSYMYYLKNGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJA9</accession>
    <name>A0A1E3NJA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_36244</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRL1 family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.3" evidence="1"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016546.1">
        <property type="nucleotide sequence ID" value="XM_019162995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJA9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJA9"/>
    <dbReference type="GeneID" id="30179682"/>
    <dbReference type="OrthoDB" id="276239at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051730">
        <property type="term" value="F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032056">
        <property type="term" value="P:positive regulation of translation in response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000622">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019039">
        <property type="entry name" value="T4-Rnl1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012387">
        <property type="entry name" value="Trl1_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015966">
        <property type="entry name" value="tRNA_lig_kin_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015965">
        <property type="entry name" value="tRNA_lig_PDEase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004:SF1">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09511">
        <property type="entry name" value="RNA_lig_T4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08302">
        <property type="entry name" value="tRNA_lig_CPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08303">
        <property type="entry name" value="tRNA_lig_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019634">
        <property type="entry name" value="tRNA_lig_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="T4 RNA ligase 1-like N-terminal" evidence="5">
        <location>
            <begin position="79"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase kinase" evidence="4">
        <location>
            <begin position="408"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase phosphodiesterase" evidence="3">
        <location>
            <begin position="578"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="2">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR019634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR019634-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="818" mass="95145" checksum="D26B1A82D9C32F48" modified="2017-01-18" version="1">MSSTQFEPIQFSAEEQQKQDNEARELVSHLLELTESVHDAKINHIRKIENVICGTDIPILSWRFSEWDYYSKKIELPTQARGLFTTKEGVIICRGYDKFFNFEELSSVKEEELRNNTKGPYTVTVKSNGCIVFISGLANGKLVVCSKHSTGTRTDLSRNHAMVAQEALEVQLKKYGKSLEDLGKLLHKYRITAVCEYCDDRFEEHVLEYKENKAGLYLHGLNMNTVEFKTYPMEKVQQFAEMFGFRETKFRKLDTFDQTLQFLKDSNKTGKFEGEEIEGFVVRCSKMVDEKANDFFFKYKFEEPYLLYRELREITKQFITQGPENLKFGKHKLICMDYMKYIMPYLVTNEKLKQEYLENKGIIELRKRYFESKNTTSMQLINDELDMIDLEEEMKKLKFGKSKPNRYVLVTLSTIGCGKTTTSVGVSNLFPEIIEHIQNDNIQSPGKDKLVLAALEVLSRKPVVIMDKNNHKTVERKQIFDGFQRLNNTIPKSKLKFICLNFIQRPPKGDKDLWDLTRERVYNRGDSHQSIKVEKDGIGNAEKIMRGFISRFQPLNTTTEPDCNFDYIIDLDVHQDNSSLQNMKKIVTALQSCTEDLDLKTPTEADYVKAFEKAKLYKPTFSKKISATKRKPVYFAIDVPQKEIIMNLMNTFNIDFFNRLVKGRRVQQDFHVTLVHSAMRKQNDTKKEFWDTYSNIFKSDISRFADTKTDIPQNKRCFLSGEYSADIQLEKVVWDSKAMCIQVKILGFQLKDEPIKLPCGIDFSHLTIGTIDKNFPPVLSGVMLKHLYGDEKEVSKIQEIKLSEEIVLTRLPLYVFMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJB1</accession>
    <name>A0A1E3NJB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017350.1">
        <property type="nucleotide sequence ID" value="XM_019161107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NJB1"/>
    <dbReference type="GeneID" id="30177794"/>
    <dbReference type="OrthoDB" id="92161at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133389" description="Glutamine amidotransferase domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="2">
        <location>
            <begin position="51"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="267" mass="29972" checksum="D61E39530DB59EC6" modified="2017-01-18" version="1">MTRVALLLLDDLLTAEGADFADLGRKLIQSAVAQYPQSDVDDLEFTKFDYIRNNDNLPELDGTYDLIYLTGSRKDSYENIQFNNKLIAFLKSVVSNADSKTKLLGICFGHQIIARALDLTTVPNTKGWEMGNTVVSIADKEYQRLNNANIPHEFVISEMHRDIVSTPFDSKQLKTLSELDVYTFGSSSICSVQGLYKRGKLLSFQGHPEFSAELTGAMIRDKFSQGAVDEEFYKDASARNQKLHEDGSDPDGGLKLQKWIAEFLYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJC3</accession>
    <name>A0A1E3NJC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein S5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_26699</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017324.1">
        <property type="nucleotide sequence ID" value="XM_019162459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJC3"/>
    <dbReference type="STRING" id="763406.A0A1E3NJC3"/>
    <dbReference type="GeneID" id="30179146"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03484">
        <property type="entry name" value="MutL_Trans_hPMS_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000017">
        <property type="entry name" value="PMS1 homolog 1, mismatch repair system component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="3">
        <location>
            <begin position="215"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="327" mass="37114" checksum="47B2ECE174A02455" modified="2017-01-18" version="1" fragment="single">ALRRISKEDVHNITSGQVITDLNNIVKELVENSIDANSTSINVTFKKLGSEGLEVSDNGDGIKVEDFPNLCRKNYTSKLENFEKLIDVKTLGFRGEALNSICNVSQVSILTAEASKAPRGYDLTFDKEGDLVAQKLINQSKGTTIKINDIFKNLPVRRLHLEKHSRKEFQKCLQTLMSYLLILTNIRIIVYNVDASGKKKIMMKTAGNSFLKDNIINVYGATGLQGLEEIDLKIDLDEKYSIKITGLLSNSSIGSGRLSKDRQYLYINQRPVEFKKIMKLVNDVYKKFNYLQYPMVLLNLEIHEHLIDINVTPDKKTILMSNKYEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJE5</accession>
    <name>A0A1E3NJE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_33190</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017377.1">
        <property type="nucleotide sequence ID" value="XM_019162805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJE5"/>
    <dbReference type="STRING" id="763406.A0A1E3NJE5"/>
    <dbReference type="GeneID" id="30179492"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.620">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009160">
        <property type="entry name" value="Acyl-CoA_deSatase_haem/ster-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000345">
        <property type="entry name" value="OLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="18">
        <location>
            <begin position="428"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ46264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="556" mass="63394" checksum="730E7B17C6F0A7C6" modified="2017-01-18" version="1" fragment="single">TEKDAQEIVSKARQYRAQKLKAAKTDAQAEIEAYKAKKAAELKKFEDEFAGSNQKLEADADTEVQTELVKIKKTAEEKKTQVVKLLLESRKSRQRVQTSHNKPSTLLRRHISERSWTLRNWYAHIHWKNLTVVAILPLVGVLLLVALHPPLVKNTFYFMVWCYIVTVISINMLYHRYWSHHSLNFCNESFVQILSIICAGGGITSAKNWCSSHRAHHRNCDVTDTDPHNIRRGLFFSHMGWMILIHHTKAARAIKESKLDELPNKQIVQWQMQHYFKLYILVGLILPSLFCGYLWDDYIGGLLYGGFLKTLLVQHSIFSINSIGHTIGSRPYNNAKSHRNNYLLSLITMGEGNQNFHHEFPMDYRNGYEWYSFDPTKWTIRLLQLLGQVNNLKIANQSTIDKSYIQQQQRVLDDIRSQLNWGIPIEKLPVFTADEFRKLAHSSKDRYLVVVSGIIHDVTPFALDHPGGVPLIRASHGKDATTAFSGAVYQHSNAAKNLLATMRIGKLGGSESLYWKQQRIENKSVPIDNDSEGKRIVRCGGQETSFGKLGSTAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJP9</accession>
    <name>A0A1E3NJP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16267</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017487.1">
        <property type="nucleotide sequence ID" value="XM_019161143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJP9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJP9"/>
    <dbReference type="GeneID" id="30177830"/>
    <dbReference type="OrthoDB" id="421448at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000006">
        <property type="entry name" value="Actin-related protein 3 (ARP3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="49206" checksum="140B905826AD6846" modified="2017-01-18" version="1">MSSINTPAVVMDNGTGFTKLGFAGNDSPSWVFPTAIATRNLSTGGSKPMAPSKPSFLSSGSGAGAGGAGSIGGGAGAGSKSNSIHGNRDMADLDFYIGDYALEAAKGPSYSLSYPIRHGQIENWDQMERFWETSIFKYLKCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVVDRSLTGTVIDSGDGVTHVIPVAEGYVIGSAIKNIPIAGRDITSFIQTMLRERGEADSSLRTAEQIKQKFCYVCPDIVKEFRKYDNDPEMFAKYVLDTVSHKKIEVDVGYERFLAPEIFFNPEICSSDFLTPLPVIVDQVIQASPIDVRKNLYKNIVLSGGSTMFKDFGRRLQRDLKTMVNERVEASERLSGAKSSGVQVQVISHKRQRNAVWFGGSLMAQTSEFKSYCHTKRDYDEYGPNIVRNFSLFSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJZ0</accession>
    <name>A0A1E3NJZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_17145</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017001.1">
        <property type="nucleotide sequence ID" value="XM_019161482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJZ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NJZ0"/>
    <dbReference type="GeneID" id="30178169"/>
    <dbReference type="OrthoDB" id="3997531at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133396" description="candidapepsin" evidence="14">
        <location>
            <begin position="18"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="69"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="667" mass="69487" checksum="6917EEA96C9C980A" modified="2017-01-18" version="1">MLGLYVLLTLFVSVGWALPLAEKDTAEKVKYWALPAKRVVRDTEVKRFVKRDENGDYLDLALTYESTFYIAEVLMGSNSQKLDVLLDTGSSDFWVVASKNSYCEAGTAGSLQKGRKISSIFGPVDNTTDTGMNKGAVSKSASDAIDCSQYGTFDQSASTSFHSNGTSFAIAYDDQTFAKGTWGQDTIRLGSVRIDHMNVAVCDNTDNAQGVLGIGLPGLEVTSSNTDTNKYEYENLPIKLVRDGIISKAAYSVYLDASSNSEILFGAVDSSKYEGELVALPIVNALASQGITSKIQLTVTVNSLAVLSAGGNKAAAIGAGAIPALLDTGTTLTYAPQNIIDSFVSLLGLEYSDSIGYYVGPCTLGDTNSVRFDFQGLQLDIPLSNFFISLTTASLQPSTQCAFGILSSNDETYLTLGQSFLQSVYMVADLEDMKIALAMSSSKKSPQDISPIDSVIPQAKNPQHTSTYGPGFNAFEVTALPSARAVDLSSVEFLHLPSSHLPFVASSVLKSTFSASTSTFASSSATVSSSSSHFSSSNAVSSSAASPAASSTAASTGSSASSLFSVSKGPSQSIQSTARSASSVSSSKVSPSASKKTLSRASASTSPLVSSSSATFASRKPLSSLRSSSISAAAASPVSSISQDKKNAGTRLSAGASGIALMFIALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK12</accession>
    <name>A0A1E3NK12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein kinase CBK1</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_35021</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. COT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016796.1">
        <property type="nucleotide sequence ID" value="XM_019162920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK12"/>
    <dbReference type="STRING" id="763406.A0A1E3NK12"/>
    <dbReference type="GeneID" id="30179607"/>
    <dbReference type="OrthoDB" id="3638488at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050708">
        <property type="term" value="P:regulation of protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000086">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000828">
        <property type="entry name" value="Serine/threonine-protein kinase CBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="303"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="610"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="131"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="691" mass="79868" checksum="D8F631BBF729A67E" modified="2017-01-18" version="1">MDGKAGSDYQGQYLGQNMAFNQANQYNEYQQQQQQYQQQQQQQQQQQQQQQQQQQQGFGFDQMQQPMSAFSANYMRQQNSVSNLSQNGSNSPHSFSQPSNMSAPALVSPQVQIQNNNGVFAQSPFYGSSPSKYSEMFTQPQPPVSPSKSLRSNNLTTVSTHSSFDSPQKSLSNNNNLNNISRMGSHSNLAMLQQAQQAQQVQQAQQIAQQRKQESEYVYFERHPEWFSKQSQDRASAVKLTLEHFYKTAIEHAIERNQRRIAFEQQLSTEISSERRNRETQLFGKNESHYLRLRRTRLSLDDFNTVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAERDVLADANSPWVVSLYYSFQDQLYLYLIMEFLPGGDLMTMLIKWQIFTEDVTRFYIAECVLAIEAVHKLGFIHRDIKPDNILIDMRGHIKLSDFGLSTGFHKTHDSGYYKKLLQQENDKTVKSARNSVLVDPINLTMSNRQQMQTWRKSRRLMAYSTVGTPDYIAPEIFINQGYGQECDWWSLGAIMFECLVGWPPFCSETPTETYKKILNWQETLVFPEDIHLSPEAEDLILRLLTSAENRLGRHNGADDIKRHPFFRGVNWEEIRNVEAPFIPKLRSVTDTRFFPTDELENVPDSPALLQAEKDRESLKNKGMDTKADLPFIGYTFSRFEYLTRKNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK29</accession>
    <name>A0A1E3NK29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">prephenate dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_33800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="2"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017615.1">
        <property type="nucleotide sequence ID" value="XM_019162846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK29"/>
    <dbReference type="STRING" id="763406.A0A1E3NK29"/>
    <dbReference type="GeneID" id="30179533"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13532">
        <property type="entry name" value="PBP2_PDT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="8">
        <location>
            <begin position="252"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="339" mass="38341" checksum="15023E0AC34361A8" modified="2017-01-18" version="1">MTRNKRVITVGYLGPPGTYSHQAAIQQFKEYESQPNTDVVYKPQKSIGACFTSVQDGAVDYSLVPFENSSNGQVVLTYDLFRDWFTSSASESAKKVVSTDTPRFTVISEQFVPIHHNLITFADDVTKITKIYSHPQVWTQCRRFLAEYELDNNIKVEKLDTSSTSKAVENLLHVSDPEERQKTAAIASSTASEIHNVPIKIPSVEDFSGNTTRFLCLGPDSAYLDQSGHQAALNFTPDTNSGTSNQKMHLLTFLIKRNDDFGSLCDILEKFKKYNLNLQTITTRPSIDKPWQYVFFVEVWDGHEPDALKACLAELEPLVFELSVVGSFYRSRRFFEMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK31</accession>
    <name>A0A1E3NK31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16356</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017592.1">
        <property type="nucleotide sequence ID" value="XM_019161178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK31"/>
    <dbReference type="STRING" id="763406.A0A1E3NK31"/>
    <dbReference type="GeneID" id="30177865"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000025">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF6">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="224"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="308"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="467"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="550"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="562"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="580" mass="65060" checksum="AC8A6C67B3915E7E" modified="2017-01-18" version="1">MSNFEDDEALFDDIYAGDQPKTVDTAPSNVTEKETSLTQQPSSYNNEAQATTDNTGNDAAVGTVPESESVNQSGSLQPYNNLPLTDSNSSNTTEQPQQNLLPSVNGQGQGQIPGQPDYATAFQQLQQFQMMQQQQQQQQPGQGQMPMHGMPQLSQYQQYQQFQNQNQNQNQNQGQGQIENQNQNQGDVQNQNQTNAHIPDITEVEVPTNYKDTVKADLSNKDVGKLFIGGLNWETDEDRLKQYFGKYGEVLETNVMRDSSTGKSRGFAFLTFAASDSVDAVLREKHILDGKLIDPKRAIPKDEQEKVGKVFVGGIAADVTPEEFRNYFEKFGTIIDSQLMINKDTGKSRGYGFVTYDSAEAVDRVTRNNYVMFHGKKMEIKKAEPRTQHLRNQGKTADGSSPMGGNQMNMMNNGGYMMNGYGDMSQYWQQMQQMQQMQQYWMQMQQQQQQNQQMSPNSQEAQINMYQMGNDNGNSNNNDDDVPNPQERGNQYRDDEEDSRSIPNLPSGPKKFREKGSGGSSGDRDRDRDRDRDRGNGGNGGSRFNERRSGGGGGGNGGGSRRSGKYRSGRRNNYHPYRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKH0</accession>
    <name>A0A1E3NKH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_133271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017755.1">
        <property type="nucleotide sequence ID" value="XM_019160044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKH0"/>
    <dbReference type="GeneID" id="30176731"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="86" mass="10424" checksum="2600E8234EDB090C" modified="2017-01-18" version="1">MLMKPFHHLPLLYSPYLIHYKEFYPKNLKGSYVYLYSVYSINLETYSFLFLPFFIYYFNSIYYNHNSSHICIKFGVIIIIISPTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKN8</accession>
    <name>A0A1E3NKN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73601</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017241.1">
        <property type="nucleotide sequence ID" value="XM_019164533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKN8"/>
    <dbReference type="STRING" id="763406.A0A1E3NKN8"/>
    <dbReference type="GeneID" id="30181220"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000106">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF29">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="62839" checksum="3AA94D231B3B76EC" modified="2017-01-18" version="1">MATEDLSDSSSFNEQDLIKGHEVDSFQQSILSEDEAPDSANNPFLNEKFREKYVNLYEDAQYECRGKFDPYFKWSKQAQRKLTFKLDFRVTFLACIFFSALQLDRGNLAQAVSDNLLEDLNMNTNNYNTGMTIFYVCFLAAELPSQLVSKRLGADVWIPIQITSWSIVTICQFRLTGKGSFYACRALLGFLEGGFIPDLVLWLSYFYTSAELSIRLSFFWTAMYATTIVNYLIAYGILHMSGVHGRAGWAWLFLIEGFITLAIGISAFFLMVPSAVQTGKPWNKNGWLTEEEQKIVVNKILRDDPSKGDMHNRQGLSLKMLFQALCDWHLWPIYLIGICAYIPENTITAYLTLVLRDMGFSTFHTNLLCIPYNFLKIIVLIVVTYFSERITNIFGTALIQPLWVVPLVGILRFWKGSLVNHWGTYVVLTIMLADPYIHAMMVSACSRNSQSIKTRTVSASLYNMFVQAGSIISSNIYRTPDKPLYHAGNSVLFAFGLAMFPILIGTKLFYMYLNKRRDKIWSAMSQEERDNYITTTTDSGSSRLDFRFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKX5</accession>
    <name>A0A1E3NKX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_16567</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017849.1">
        <property type="nucleotide sequence ID" value="XM_019161259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKX5"/>
    <dbReference type="GeneID" id="30177946"/>
    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="13">
        <location>
            <begin position="60"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="14">
        <location>
            <begin position="425"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="560" mass="63860" checksum="C58945ABDEB8F0C8" modified="2017-01-18" version="1">MLITKRHFHKSSANLAFNLRTILKENKSDGTGNSKCTDHHEHEHSHHKHSPLLVQPDYPVRTRFAPSPTGFLHLGSLRTALYNYLLAKSTDGQFLLRLEDTDQNRLVEGAEDNIYDTLKWLDVGVDEGPIVGGPYVPYRQSERSEIYRKYSDKLLQEGLAYRCFCSKERLDGLRDSARLLKPPTTVSYDRHCLEHISLEESNLLAKGGKSFTIRFKSPDSYPEFTDLLHGTINLQPQVNPTDRRYEDPVLLKSDGLPTYHFANVIDDHLMKITHVIRGEEWVASTPKHIALYNAFGWEKPNFVHIPLLTTVSGKKLSKRSGDIDIMSLKKKGYLPEALVNFSVLFGWNPKRELGEKTSEIHTLDELTKTWLLSGLTKGNAKVDWKKLDFFNKHYLSEKLKDSSSDFFKTCVAESHQNLSRTLKLDNLDVTKVEEVLKMIYPSLTTVNDFDTEQYHYFFVKPAYDKSELLSKLRVNEDVLKVISQELVSHLDKLTHSSFNATIGEILADIPSLKKRAVFQTLRYALSGPQSGINLPVILELLGPAEVNERVVAFDKYLGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL80</accession>
    <name>A0A1E3NL80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_16228</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10 11">Cytoplasmic vesicle</location>
            <location evidence="10 11">COPI-coated vesicle membrane</location>
            <topology evidence="10 11">Peripheral membrane protein</topology>
            <orientation evidence="10 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10 11">Golgi apparatus membrane</location>
            <topology evidence="10 11">Peripheral membrane protein</topology>
            <orientation evidence="10 11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017441.1">
        <property type="nucleotide sequence ID" value="XM_019161130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL80"/>
    <dbReference type="STRING" id="763406.A0A1E3NL80"/>
    <dbReference type="GeneID" id="30177817"/>
    <dbReference type="OrthoDB" id="10266042at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090167">
        <property type="term" value="P:Golgi distribution to daughter cells"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09254">
        <property type="entry name" value="AP_delta-COPI_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14830">
        <property type="entry name" value="Delta_COP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000003">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027059">
        <property type="entry name" value="Coatomer_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121:SF0">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="14">
        <location>
            <begin position="283"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="230"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ46328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="537" mass="60551" checksum="F77017FAEA688490" modified="2017-01-18" version="1">MVVLAASVCTHGGKLIISRQFRELTKERVTALLSNFPALLSDSTQSQHTAVEDEYVRYMYQPLEEFYVVLITNKHSNILQDIDTLHLFAQSITSILRTVDEKEIYENCFELLSAFDEIICLGYKENLTSSQVINFLEMDSHEERIQEIIERNKELEAAEASKKKAVELQFKEMMKKSNMQGMDQYSQPVQTYNPPPVANDRDDYTTYAEEQHSSLSSQNQIKRGGMQLGKKSATFGDSQPLLVSTPAPQRRAVSQSQQPQYQQQQQQYQQQHQQQSSKPIIDNNGILLTINEKYSGQITREGTITSAEIKGDLQIRINDPSLAHAQVKLTLQNDSDITTQYKTHPNVDKKLFNSSSIIGLKDPTKPFPSNDQNLGVLRWRSTRKSFDEEGSSGLLPIVLTTWVNNNNDGTVGLTFEYEVDTSKNVDEVSIIIPIINASFESSDNDNAALEYTDSGVNIKLTDLLKYESGSFEILCKDVEDEEALFPMELSFDELKKIEASGSEGIQVSVDSVVNSESQDALPFDIYINSSSESYYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL99</accession>
    <name>A0A1E3NL99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72426</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017461.1">
        <property type="nucleotide sequence ID" value="XM_019164193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL99"/>
    <dbReference type="STRING" id="763406.A0A1E3NL99"/>
    <dbReference type="GeneID" id="30180880"/>
    <dbReference type="OrthoDB" id="294295at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF8">
        <property type="entry name" value="7ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="293" mass="31873" checksum="2A2944B2D253EA16" modified="2017-01-18" version="1">MSAFTIENIYEIAGRQAIVTGGGSGIGQMICQGFAANNVDVAVVDLFQDRLDETVRLCEEAKKETGSTAKITTLCYDIGNEKSNAALVADINSHYENVDILVNCAGIRRQNQTTFTPGEPLEKLAEATNSIGWKDFRDTFDVNVFGMYFLTAGLVQKLGEAAAKGDGRGCVLCFSSPCSVHNNQFVPTYQLSKVSIDHMVRIMAAEFADKYIRVNAISPGLYASRMTPMSPDDASSNMKYVHLVPARRAGTAEEMVACVVFLCSKGGAYMDGRNLRMEGGRLLTLKGHIYSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLM3</accession>
    <name>A0A1E3NLM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017581.1">
        <property type="nucleotide sequence ID" value="XM_019164227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLM3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLM3"/>
    <dbReference type="GeneID" id="30180914"/>
    <dbReference type="OrthoDB" id="4076200at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012917">
        <property type="entry name" value="DUF3294"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07957">
        <property type="entry name" value="DUF3294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="220" mass="23858" checksum="8C1EAC5150CC2A2E" modified="2017-01-18" version="1">MSKTSIEDLQEQINDLKSQVVKQRAIIAKTAQQMLSMQVKDTKQHMDSIPDPLGLGGAGKAGGGAAGAAAAVDTADFATNEDLVQLVGELQGQLNLLEQKSVLRTVNSHQHRDSDVLLALPNLDGDMPASEVFPHSVAEFVNLGDDVVVQLCKFYQLLPPTLEERARMQAFVEGKIASPNEPLNGDYEPPADDYPPETLAELFVELANFIGVSHIKKRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLU9</accession>
    <name>A0A1E3NLU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_71228</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018225.1">
        <property type="nucleotide sequence ID" value="XM_019163865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLU9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLU9"/>
    <dbReference type="GeneID" id="30180552"/>
    <dbReference type="OrthoDB" id="10265243at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140018">
        <property type="term" value="P:regulation of cytoplasmic translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="85"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="399"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ47112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="551" mass="63147" checksum="E755BB61B80958C4" modified="2017-01-18" version="1">MPKGPKTHQKLPSEKERFIQACADISLELVSSLGTSKEVNLNGVIIKFSKKYKLKQQPRLTDVINSIPDQYRKYLLPKLKAKPVRTASGVAVVAVMSKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQSRGRIEQLRQLGHSIDKVEYIIMGGTFMSLPKDYREEFIVQLHNALTGYNGTDLDEAVAFSQQSQTKCVGITIETRPDYCTETHLDDMLRYGCTRLELGVQSVYEDVARDTNRGHTVKAVCETFAYAKDAGYKVVSHMMPDLPNVGMERDLEQFKEYFENPAFRTDGLKIYPTLVIRGTGLYELWKNGLYKSYNANMLIDLVARIMALVPPWTRIYRVQRDIPMPLVTSGVENGNLRELALARMKDFGTSCRDVRTREVGIQEVHHKIQPDQVELIRRDYYANGGWETFLSYEDPRQDILIGLLRLRKASKRYTYRKEFKKQQTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAKEEHGSSKISVISGVGVRNYYRRLGYELDGVYMSKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLZ8</accession>
    <name>A0A1E3NLZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_16261</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017476.1">
        <property type="nucleotide sequence ID" value="XM_019161141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLZ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLZ8"/>
    <dbReference type="GeneID" id="30177828"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="214" mass="23892" checksum="8D5AFF97E15231C9" modified="2017-01-18" version="1">MQPADTNTPCVLHAPLLLFCRIIRDRTTSRGDFVFYSDRIIRLLVEEGLNQLPVDPVKIETPLGVTYDGHKFQGKICGVSIIRAGESMEQGLRDCCRSVRLGKILIQRDEETATPKLFYVKLPDDISERYVLLLDPLLATGGSASMAIDVLAERGVKPERILFLNVLASPEGIQVFKEKYPAVKIITGMIDEGLDDKKYVVPGIGDFGDRYYCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM12</accession>
    <name>A0A1E3NM12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_31031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018270.1">
        <property type="nucleotide sequence ID" value="XM_019162670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM12"/>
    <dbReference type="STRING" id="763406.A0A1E3NM12"/>
    <dbReference type="GeneID" id="30179357"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="118"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="359" mass="38865" checksum="FBBC37EB535EC182" modified="2017-01-18" version="1">MSIFETKLNDDYSWPEPFAALPLALDASMVDKLDPEYVAFFREVLCERPDILETHKYPVAHTKAGGNVIPGQAPALAVADTYDIAIPRKHTQPDAGSIPARVFVPHGEPPAAGWPCTIWYHGGGWVLGSIDTENSYCTHLADRAQCVVIPVDYRLAPEFPFPACVHDSFEALLYVMENASALGIDNTRVCLGGSSAGGNLTAVMCHRYAASPLARGLPPIRFQLMVVPVTDNSATGETQPSWAENALTPQLPAAKMVWYRKIYLPDAGATLTDPESSPLFYPDESFRHVPPAFIAAAECDVLRSEAEAYDAKLRANGVPSTLHVYKGVPHTAMVMNARLTQGANLVRDTTAAVRAALHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM17</accession>
    <name>A0A1E3NM17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_15173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018297.1">
        <property type="nucleotide sequence ID" value="XM_019160699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM17"/>
    <dbReference type="STRING" id="763406.A0A1E3NM17"/>
    <dbReference type="GeneID" id="30177386"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000013">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="243" mass="26646" checksum="E6634D68E19F08DA" modified="2017-01-18" version="1">MVNVISKKRKLVADGVFYAELNEFFTRELPEEGYAGCEVKITPTKTEVIIKATRTNAVLGEKGRRIRELTMLIQKRFKLAPGSIILYAERVQNRGLSASTQCESLKFKLLNGLAVRRAAYGVIRNVIESGAKGVEVVISGKSKGARAKAMKFGSGFMIHSGQPVNDFIEVATRHVLLRQGVLGLKVKIMTDPALNKKGPKSLPDYVKVLDPKEDERIEEPSVIVHKSVARAPSAEATEVATEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM20</accession>
    <name>A0A1E3NM20_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">[Histone H3]-trimethyl-L-lysine(4) demethylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_15113</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018229.1">
        <property type="nucleotide sequence ID" value="XM_019160673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM20"/>
    <dbReference type="STRING" id="763406.A0A1E3NM20"/>
    <dbReference type="GeneID" id="30177360"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="10">
        <location>
            <begin position="17"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="306"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="11">
        <location>
            <begin position="473"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="228"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="743"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="834"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="755"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="834"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="856"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="953" mass="107686" checksum="E84EDB3B19F27B39" modified="2017-01-18" version="1">MGNMGNMDDTVIGLRSVPELRPSAEEFVHPIRYLSQPEVAEMGEKYGILKVVPPAEWRPRFSLDWDTFKFHTRIQRLHELNLRNRSRACFVEGFNCFLLSLGEDPLETTEDLCLGVSDEFPLRPAQLRRTHLNGWMELKDGSRVHVHDIFISREYRRWFYRVHDTDKELLKRLTTYSKRLLSMLGLAEGSPSEAGAEPSSLAVPAEVSHTTLRRLMVSPAALLAQPANLKRLQRMKSRMAKSDGGSRKRRHISEEDPLTPPAEKPQQFKADGDTRPTRTMRTTRTTPTTKPFFPLTPEIVSPEALDETCVVCHSSDSPETTLLCDGCSRAFHMRCLPVPLERVPKYDWFCGGCLAGSTGIYSDYGFEEEFENRFSLNEFRKYCQEWEIEFLQLLRSGELGAELKLKPEETMNQLSETTIEKVFWYLTNGKMRIPSGLDSMKIRYGADIHSSVPGELSGFPSADNPRMQEEDREYIQSEWNLTRLPFAQGSLLEYVCGSMSGGSGEAGKEQISGMSIPWLYVGGPLSTFCWHKEDHYTLSANYSHLGAPKKWYGVPAKDCGAFEEIISGIAPEYEAKQRDLMHQLVSMVSPDELRRTSAAGQRRLEIYETVQRPGEFIITFPKVYHSGFNYGFNVNEAVNFTLPLWVPYSVSAVDEYERVSKECVFETFALLRKILADLATDAGCAQWMRDTGVTRQATDDVRRWVSDKYHGEVRRFLGALEAPGLADALRGMKRVSLREYLDERPAAGGASGPGDSDEDEDEDEDAEGDEVDDKLCTDCKTRVHFQWVLVDLYADCLAQLPGAGAADAPLPTAGLGIRHRDVESEWRAIIERAEEQQQQQDRGHGDRPGDGDRLRRSSRRIQTKRLRPAAPTADEKERRELRGSLLALRRENAFFGTCVFCVRCFGLELRRLGPAERTRAVAAAVRVEEYTTAELRHAAELCTVAAAPPAQPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM48</accession>
    <name>A0A1E3NM48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_108797</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the eukaryotic ribosomal protein eS32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018327.1">
        <property type="nucleotide sequence ID" value="XM_019159725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM48"/>
    <dbReference type="GeneID" id="30176412"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007836">
        <property type="entry name" value="Ribosomal_eS32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05162">
        <property type="entry name" value="Ribosomal_L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="135" mass="15662" checksum="C47F6C13EF2F64F3" modified="2017-01-18" version="1">MRRGLPDLFFTCLGPAWAWPRRRAACSGPAPRRTPHTARPELPSPPRLPALSGPPPRPASRRSLPHYADELPIYNTHRFHLHCSSGLQRLSLPPPWLFFSSFPSATAYPTMRAKWRKKRVRRLKRKRRKVRARSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM81</accession>
    <name>A0A1E3NM81_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">threonine synthase</fullName>
            <ecNumber evidence="4">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_16344</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="4"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017566.1">
        <property type="nucleotide sequence ID" value="XM_019161174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM81"/>
    <dbReference type="STRING" id="763406.A0A1E3NM81"/>
    <dbReference type="GeneID" id="30177861"/>
    <dbReference type="OrthoDB" id="5203861at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01560">
        <property type="entry name" value="Thr-synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000024">
        <property type="entry name" value="Probable threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1380.10:FF:000003">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051166">
        <property type="entry name" value="Threonine_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690">
        <property type="entry name" value="THREONINE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24857">
        <property type="entry name" value="THR4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="10">
        <location>
            <begin position="92"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="511" mass="57136" checksum="FB07C75430A47930" modified="2017-01-18" version="1">MSQVYRSTRSAAEQAITFEEAIITGLAKDGGLFIPSEIPQAPKDFLQAWENLSFEELAFEIMRLYIADKEISDADLRQLIEKSYSTFRAKDVTPLVKIDSKDNLHLLELFHGPTYAFKDVALQFVGNLFEYFLQRKNASKKEGEQRDIITVVGATSGDTGSAAIYGLRNKKDVSVIIMYPTGRVSPLQEEQMTTVTDDNIHTFSVKGTFDDCQSLVKEVFADVKFNDTYHVGAVNSINWARILAQMTYYFYSYFQLKKQVGTSDFHVKYVVPSGNFGDILAGFFAKEMGLPVDKLVVATNENNILHRFINSGKYDKVPVKVTYSPAMDICVSSNFERFLWYMIKKTDGGNDDLKTGEIMSNFMAELSSTKGEFNVSQETVQAARELLDSASVTNEETVATIKETYDSTSNKYILDPHSAVGITTCLKQIDAAQDAPNTHYISLSTAHPAKFSEVVNLALGGFEGYSFENDVLPAELKQLSTMPKKIRLIEHADLKTIEDAIVEELAKEKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMT5</accession>
    <name>A0A1E3NMT5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Decapping nuclease</fullName>
            <ecNumber evidence="7">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_76503</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside-ribonucleotide in mRNA + H2O = a (N(7)-methyl 5'-triphosphoguanosine)-nucleoside + a 5'-end phospho-ribonucleoside in mRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66928"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17313"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172876"/>
            <dbReference type="ChEBI" id="CHEBI:172877"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:66929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60880"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:60881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78683"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78684"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018535.1">
        <property type="nucleotide sequence ID" value="XM_019164949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMT5"/>
    <dbReference type="GeneID" id="30181636"/>
    <dbReference type="OrthoDB" id="3989136at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034353">
        <property type="term" value="F:mRNA 5'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="8">
        <location>
            <begin position="24"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="360" mass="41944" checksum="82197774D7E19204" modified="2017-01-18" version="1" fragment="single">MPEYIIELEIPRTDVSSLSKFYGKEVAFFSDRAGTVTLDKEQFDFYSFSPPNSKCFANPFGWNTKLGYKNYTPKIFKSHESTIDNFLKSLVQISKVQNSGVNLSWYKVFCPSSFFQYVSVKRQSGFYIMWYNNRYIIANNETREGQNVLMSYAGIRFEDLLTHGTSQKTINNYNLYQSVIETKIDGMSCLYCAEIDSYDKESGAYTEIKMILCKSNIPHAKTTNKKSVLESLSKGNVHFGSFLFRLLIQCKFANDLKVVIGIRDQSFTIRNITDFSVKDDLLPFFHQLYPDYYEKYMAAVDLIRRVFHTITKRVTRENPVFYLEIGSQYILKSVDSMNERCRILKRFVPTEFINLIDSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMU1</accession>
    <name>A0A1E3NMU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-hydroxy-4-methyl-2-oxoglutarate aldolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018554.1">
        <property type="nucleotide sequence ID" value="XM_019160804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMU1"/>
    <dbReference type="STRING" id="763406.A0A1E3NMU1"/>
    <dbReference type="GeneID" id="30177491"/>
    <dbReference type="OrthoDB" id="1476984at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047443">
        <property type="term" value="F:4-hydroxy-4-methyl-2-oxoglutarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.40">
        <property type="entry name" value="Ribonuclease E inhibitor RraA/RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254:SF28">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605493-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26282" checksum="05A78BD4543DEDC3" modified="2017-01-18" version="1">MPYETKKLIEVLSKYATCDMSDALVKHKHKYGGSLPNLKRFSSNSQGKTMVGKAYTVLFAPKDDPREAIAGGYIDKLPEDSVLVIATTMNLQKKDAPFTKMSNALYGGLMSTRAQYLKSTGTVVFGRVRDIDEHLELHRDVFSYGLGSAAHGPVVKMVGINVPVEVLIDGYPEPFTETINPGDIIMGDDNGVVKVPHDDELESILEYIPKRVQADNLVKEDILNGEACNPAQKKRRAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN72</accession>
    <name>A0A1E3NN72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="3 15">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_71603</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="3 15"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018658.1">
        <property type="nucleotide sequence ID" value="XM_019163962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN72"/>
    <dbReference type="STRING" id="763406.A0A1E3NN72"/>
    <dbReference type="GeneID" id="30180649"/>
    <dbReference type="OrthoDB" id="258806at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
        <property type="entry name" value="TRM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="17">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ47545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="482" mass="56126" checksum="5B2FBAC9AD0D55E7" modified="2017-01-18" version="1">MSKVEELKRNEQGTTSSKRQRTDVLKKTPPESKVIYKCSFIKPKNNKKCGLQVRKGQKYCFAHINLVGRSENTESSKVSDVESDITRISKKGEIVKRVKCTVDPSHTVWEDHLSKHVKICNMVKKKNEVEEKEKTCPWFRINYNVRDAKGEEVNVTSKDIDHDEWKRWIPKWTLQHDLIFLEELPLKNIEFKDGLEERFAELSNQKHIRQQSSLIGQLIEHEIVVKDADPGVVIEFGCGRAEFTRYFNKAMGRYLDHSLDNSAKYLLVDRDNPRLKFDKKIVHDSAEAEISGVKLERLKVDIKDLKLIESLKSFRENNDGNSVMRYIGISKHLCGVATDLTLRCLINTSKDYETEKERLKFGGCLVAMCCRHCCKYDWLLCESKSYLKENFGIDETNFVYFRKMFAWATSGVNPQMSKTDTGDHFTGLSFEEREKIGLKMRRILDESRKYAMEQKGFDVQIVRYVEREVSLENNCIIIKNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNI3</accession>
    <name>A0A1E3NNI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018331.1">
        <property type="nucleotide sequence ID" value="XM_019160712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNI3"/>
    <dbReference type="STRING" id="763406.A0A1E3NNI3"/>
    <dbReference type="GeneID" id="30177399"/>
    <dbReference type="OrthoDB" id="5398391at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37987">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37987:SF1">
        <property type="entry name" value="OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="196" mass="22166" checksum="4456F5616FD7507A" modified="2017-01-18" version="1">MQLPDPTQMGKLSDTQLTAVLDNLFEPCATLNAYLLPYVRSTSFGTYTELIELCRIELIKLLERYKAGMDKPEFEEEQELRTKICKIVSAHPRLGVPKKQAATLSVHSQNEQKSLSAEGAGGEELARQLQSLNAEYEQTFPGLIFVVFVNGRSREEIMKNMRYRIAHSNWLDEVNTAFNAMADIALDRAKKLNAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNR8</accession>
    <name>A0A1E3NNR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_15631</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018830.1">
        <property type="nucleotide sequence ID" value="XM_019160898.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NNR8"/>
    <dbReference type="GeneID" id="30177585"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="318"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1321"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1373"/>
            <end position="1388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1630" mass="181893" checksum="198C727A9BE76181" modified="2017-01-18" version="1">MDIARPVGSSITHVDFGILSADEIRKLSAKQITNPTVFDNLGHPISGGLYDPSLGAFLKNLCTTCGLSDIYCPGHQGHIELPVPVYNPLFFNQLYMFLRTSCLYCNHFKLHSNEVHRFECKLKLLQYGLILEANEIDKIRVDSNDLDDTANDIEDADADSESANISVSSSRLLREKRELYVLESINNAIKEGRSTKAGIFTATVGEARKDLITDFYSKVLGKKKCENCGLFSSTFRKDGFSKIFENALKDKEATNNRIKLGSIKGKKSFIHTPPKAGSKYVLSTEVRSIMRALFNTEASIIDFLFHSKPLSAQSLTADMFFIQALVVPATRFRLPSKLGEEIHENSQNELLTKVLNTSLLIRDLNDQFSSLTKDLSIDEKKIIFNRLMNAFVTLQNDVNGFIDSTKNQNVSSKAPTPGIKQALEKKEGLFRKHMMGKRVNYAARSVISPDPMLETNQIGVPPVFAVKLTYPEPVTAYNVSELRQAVINGPDKWPGAIQVQNEDGSLISLIGMSLEQRKSIASQLMTPSSGFTVVNKKVHRHIRNNDVVLMNRQPTLHKASMMGHKVRVLPGEKTLRLHYANTGAYNADFDGDEMNMHFPQNENARAEALNLANTDSQYLTPTSGSPVRGLIQDHISAGVWLTNKDTFFTRERYQELIYSCIRPEDGHSSGSRIITVPPTIFKPVPLWTGKQVISTILLNIKPQDTPGINLKSKNKIKNDYWGEGSKENEVLFQNGELLCGILDKSQYGASKYGIIHSLHEVYGPDVAGKALSVLGRLFTTYIMMIGFTCGMDDLRLTGEGNKWRNEILKESVDTGRLAATEVTNLDSSTKVNDPEFKKRLEEILRDDDKLAILDAVTMSKVNSITTKVVSTCVPDGTMKKFPYNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPCFKPFDTDARAGGYIKNRFYSGIRPQEYYFHCMAGREGLIDTAVKTANSGYLQRCLTKQLEGATINYDNTVRNSDGTMIQFMYGGDSVDVTKESHLYEFKFFVDNYNSLLQRYSPSNVDNLDAETAIHYNKKSRKYLKKQGDLPHYAESYKYDPTISLYNPARYLGSVSDKFEDKLDAFIESNDKLFVSSKEAKNNGGLTSKKFKALMQLKYMRSLIHPGEAVGIIASQSVGEPSTQMTLNTFHFAGHGAANVTLGIPRLREIVMTASASIKTPQMTLPILNDVDDESADAFCKSVTKIKFSEFIDDVSVIETTADVSSGINATRSYEIKIKFFDSKDYQEEYDVTKEELENTVRRQFIYQLESVIVKELRKQSIKNNASMPDIGVAVAKTKATSKITNDEGTDEKSRTKQAVSYEDPDDEELEAMKQAEKSDEELIDASASESSGDESESDDEADDEEADKMAVDIKKDENSGKKNLSKTAKVRQHEVIVGHTFVTEYDFDDEDGSWCEFKLELSADTQKLLMVNIVEDVCKKCVVREVPNIGRCLRPTAEQGVRQLVTEGVNFQAMWEHDSFINVNDIRANDIAAVLRVYGVEAARNTIVNEINRVFGTYAISVSSRHLDLIADYMTREGTYLAFNRQGIDSCTSSFMKMSYETTCQFLTKAVLNSDREDLKSPSARIVMGKLNLAGTGSFDLLSKMPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNX2</accession>
    <name>A0A1E3NNX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018491.1">
        <property type="nucleotide sequence ID" value="XM_019160777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNX2"/>
    <dbReference type="GeneID" id="30177464"/>
    <dbReference type="OrthoDB" id="10344975at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="306" mass="35428" checksum="E7F3C331B1842064" modified="2017-01-18" version="1">MVENGRSFEKIARTDVKDLRFNAEPLVENVNEPTGEDERKFKKYASEIRARNKYENPLNREFAIYKDLPETKAERKNLQCFKHEQVSSSKIDLQHSKLQKKSNKYGGLRMISDTVLTKRNFSSDNELNPTKQAELSESSGFPEDTERAENLNQIYSKNYIAPEKTFERFRAPLQRKPLALISPFAGHDEIFNSERTKLLAANAKQESDYSDSDLDNSSDKENIYPSIELEKPLIGSDDSLESQKYGDSSSKYGAIHLFTPQHYWNLTFTRKQSGTTVSLEDFHLLLYDYERLWDRYVGDFAKDTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNY4</accession>
    <name>A0A1E3NNY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Signal recognition particle subunit SRP72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71819</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SRP72 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018900.1">
        <property type="nucleotide sequence ID" value="XM_019164016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNY4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNY4"/>
    <dbReference type="GeneID" id="30180703"/>
    <dbReference type="OrthoDB" id="5421607at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013699">
        <property type="entry name" value="Signal_recog_part_SRP72_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026270">
        <property type="entry name" value="SRP72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094:SF9">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08492">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038922">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="Signal recognition particle SRP72 subunit RNA-binding" evidence="11">
        <location>
            <begin position="536"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="531"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="601"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="545"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="629"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="640" mass="71303" checksum="557EE9368A13EC22" modified="2017-01-18" version="1">MSALTDLFGKLEVYSKTDSHRQVYDTAKLILQKDKTNVSALKRSLVALINMDNYTDAKKLLQQHKALVEANRGALVLELAYVHYKVGSREEELVQLGEFTTPSTVRAFKHILAQYYYRTGDDVKALELYQLLIREGGSDSLESLDLTVNERAVISQLKFRNTFPYTFGGHTEPVSAPHADSYDELFNESLILVIDGKYQDAQASLNRTYDLAERSLADYEPQEKFNELAPIKLQKAYLHILMGKYGEANALLDELNGELESVDSAGDVNARILKLIVQNNKLVCVQNLGSLDGVEPALLYREMDLQDAISLSKPRLTLPQLAIIERNHLLLSFLSGKQSKKSIKHYSEDFPSSLLAEALSSGITKEDLKSSNKKLFYSLSTKNPSNLSLALLSTQVAINSSNYQRAIALIENVVNHDEKSLLLPSVGKLLYSLYELLDCKKLIHQLFLKIQKLFMQKQNETFSNEDIKYAKFVSLKLLATDESIAKELLEKIDELDILSAKYSSADIQSLVAGVDIDVIVSEGITPLITSSISSSTQSNSSKLNKVSKPKRKRSTPKKLAKDTAQQIDPERWLPMKDRTYFKMKKSKKLKNTQGGIIDAATEKALNIGGSPSTVAAETKTSTTSGKPSSSKKSKKKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP46</accession>
    <name>A0A1E3NP46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_10809</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018974.1">
        <property type="nucleotide sequence ID" value="XM_019159719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP46"/>
    <dbReference type="STRING" id="763406.A0A1E3NP46"/>
    <dbReference type="GeneID" id="30176406"/>
    <dbReference type="OrthoDB" id="5392646at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048401">
        <property type="entry name" value="SLS1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048400">
        <property type="entry name" value="SLS1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20778">
        <property type="entry name" value="SLS1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20776">
        <property type="entry name" value="SLS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLS1 N-terminal" evidence="2">
        <location>
            <begin position="182"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 C-terminal" evidence="3">
        <location>
            <begin position="432"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="838" mass="96009" checksum="822B3E63AF9298D7" modified="2017-01-18" version="1">MLGRYKTILKPRIALPVPIYPTLRNPVSRRWNSTSRSASSIVQESNNHNDNENKGEEDHKQYESETATETEQVHSGEKIGRHQSVEPEFMSFPWENTSKENHDMEEDLPWASKNEAEAEPILEIDNTDPSKSELILAPLRKKSSPIERLTPRKSVSHRADRSSGVNFQIERDYKNLMQFKYNVSSDDFVNIIEELKPSTPVVTVKQLTELSLNLDKSFRVKQIRQYIENSCPEIVLKKSMSKKKLINKIISEHWNLKVTLDPTGDLVCETRIDLSNKRDLFLLLSNRGFLPQHWSLIGAQLSLGTTRKELVVRGSKNIVNFVQASWNDLLNNISTDTVNLGPMMDFYESLGKTLDLNTLQHECGVHFDKVSLDKKSYVLSAIKNASISNAKIEILKATEYRRGCTNSIITELIKEAEKEDKDMLMKMEVSDESLPWYIKPENYFRYSITKERLRESLLSDDSMIVESLSDEVQDLKLEVEEAREKFEYNYVQTEDEWNKKKLEKSKRSDEKFFHIKEGSRSSTEVLDKLDAAKIEEGLADGDFGGRDDPTKLMLDNVVSVKFGKLLFEKEKVPESVAETPRNTYFSENLSQVVRNIGRLELMDKDARLFGATGGIMNRFNRQIHVKLIPNGFWGGRFENFVESPSIEILFDLKDGKLTVNGFSAFTCESERSVEVAVPHLDVDMKFQSSYNSMLVYNRDQWLLNGRPEHAFRGLEGAATDNDITLNKRQINMFISQIAKRQLDLRRPGGLLAMLHQFRHKPYAFHFPQGPNRTNLVKYSAVKVELVRSIELDYAGLPVSFELHDDGEHQRVEVSMLRDGVDAAHFVRHSAGLAALLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP72</accession>
    <name>A0A1E3NP72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_23531</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018446.1">
        <property type="nucleotide sequence ID" value="XM_019162310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP72"/>
    <dbReference type="STRING" id="763406.A0A1E3NP72"/>
    <dbReference type="GeneID" id="30178997"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000919">
        <property type="entry name" value="NPR1p Protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF113">
        <property type="entry name" value="NITROGEN PERMEASE REACTIVATOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="357"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="183"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="251"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="736" mass="79905" checksum="057E499C9F5CF867" modified="2017-01-18" version="1" fragment="single">GVSSLTKLMNEQKQKPSSPLSLQINTSDSSSHMNSYNGTITTAVSVGTSPDEGEIDEHTHPGLLSHSSKTSVSSFKNSPSTSSLFKQTNSFTQLSSSIPYSVPGNHKPSTSGTPSSSSFTNMPVNGQFITGTQLSSPTVASNQIESRFVVSKQKIEARGLSSSVNTNSKSSSSLANFFSKSRRSTATNFDSLPPSPVTYSSNNNMSNYSANNAPQHDLHSPSSQSSNESNSSVSTRHSSMADLRRFFKRSASISNAPPSSPRVSNLSAGLSSQRQSSVSNTTNGNSYTPIQVPPVSAGSISQLSTLSNTPNSTHFRNPNFIGNNPPSSSPASSSSNYTQHLSVGGNSTSIPFSKRYVKFGESLGAGAGGQVKLVKRLSDQKVFAVKEFRAKFVTENKRDYTKKITSEYCIGSTLKHPNVIETIEISFENDRLVQVMEYCDYDLFAIVMSNKMSEEEVNCCFKQILNGLRYIHSIGLAHRDLKLDNCVINKHGIVKIIDFGSAVVYQYPFSNMLIEATGIVGSDPYLAPEVCVFNTYDPRPVDVWSTAIIYCCMILKKFPWKIPKLSDASFKAFACREPGVTFGELLKRIPEPPSYDAELNDSSTTIPVLKTEVIEAHEVETPQIATVGNTTDGGNVTTEDGAKHTSNLIGEERLLNALSSEVRPLISKMVRLAPACRITVDECFEDPWLESVQMCAMVDENGESSCDGKLIPANDHEHTQVDQSVAHIASLERAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPC7</accession>
    <name>A0A1E3NPC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019018.1">
        <property type="nucleotide sequence ID" value="XM_019160962.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NPC7"/>
    <dbReference type="GeneID" id="30177649"/>
    <dbReference type="OrthoDB" id="71307at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001280">
        <property type="term" value="P:positive regulation of unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001880">
        <property type="entry name" value="Mga2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057962">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23335">
        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR CAMTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23335:SF1">
        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25603">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1200"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="7">
        <location>
            <begin position="637"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="843"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="876"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="392"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="537"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="759"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="933"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="983"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1069"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="413"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="759"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="939"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="984"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1009"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1071"/>
            <end position="1083"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1286" mass="143412" checksum="12E37E0F879A1FF1" modified="2017-01-18" version="1">MDTFMTFISSGAYPGKREPERTRERDRTTDREGASRMEDRAELCTPKDRQPEGGLDEGEIARQILEDEVLNEFLNPQCFQQPNEILDMIDGTQEGANNGNDAVDTTVGSEYGSNSQMVPNGRVLNPSEELRMYEGSKKELMKVKFGLNQDAKYIHHSNVGFSYTRAPAVSSPDDPEVTTTLLPPAYQECFLRDEQSFPYHIQIADLPSHSRVETQIKLELSISPPPPQFLLHIPRDSITKPKFTLADNTLPDSIQDHLLYLDLFIVGSQTSNANDGRVKSCNVCKRCMRRELKRASRRKAGLVDDSSNWDINLPKRAIIINSKEIVSFPAPNGNTNERKIELLSRIVCYCRHHQELEGFQILIFLKNKEGAIVGKTISTPILIMDRKKSLKSSMERNKENVLDQEENGSDLYTKSSTMNKKNSSITSNNSNGDSSAELSVPTSTTTMNSVSNNNPATASSSSLFNPISSVTNMNSMNLLTSNGGKSQTGMFSSIFSFDPSNLLGDENSRQVKRQKRPWSPSESSTYFETINQQRGIPSAIGDSKKNTTKRDPISPISSDAISPQNHLFSSATSAFSTTPEKEYREAATTLSPHDSNILVDPSSNLISTNSSISAGLNVNMGLDIDMNLNMNVNNEDCPTIRRIIPSQGPIRGGIEITLLGSNFRPGLIVKFGRNVALATQCWSDSTIVTYLPPASQAGPVLVTFEDANYDFTNSNAHQIFTYTDDTDRQLIELALQIVGLKMNGKLEDAKNIAKRIVGSNQMDSSQQSQQQQHQASPNSTMSLADVPLNQQLQLNWMAVASNKIKELSKSTLNHEEILIKFLSMIKIPNSLISSPNWAVCNSEGQTMLHLACLQNYSKLCHFLMKNGSRVDYKDRNGFTPLHFAFIGGNRKIINLLNKFKANTSSKLENGVLLSHIADSNVLDLIHASRRSSTSSSDINFDEDDEDNENMDLSFENDFENDTDLSYDAAIVTEELDDYQLSYKTNRHKGKRSVHHKSKTFTRKHGNVDSEFEADNEDDTDSIPDKVEQIADHEKKTSLNDKPTNSFGINLWIAMKEAIKNKMVEISVGQEKHKKTGHERHGSKKQMGVKASNRGEQQVGSVVESEKDVSSEDHLPSYDDLFPQGTSLRSLINFRGHADDEQEGQKKAAPTRRVKESLLSTGNCDVINPDGGVDDDDEADSDVDMIAMNLRTSINSDTKLMFFWLPCMLLLLLIVIGNNLNIITIQKLQFFSYFLEQVREIVGSFMLGKDRFTNLLNENINYGRERMENLINDVNDAMMTAVAGVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPN8</accession>
    <name>A0A1E3NPN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_21016</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018626.1">
        <property type="nucleotide sequence ID" value="XM_019162181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPN8"/>
    <dbReference type="STRING" id="763406.A0A1E3NPN8"/>
    <dbReference type="GeneID" id="30178868"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF3">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="6">
        <location>
            <begin position="111"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="577" mass="66833" checksum="D5B70C5F95435D77" modified="2017-01-18" version="1" fragment="single">KLPPGIKIKNNSIDNDRLIMDKFGHTHFIGNFGTASLLNGLCDIIIKRSYNQQNPPTSTQDIRDSSVQTITSENVPVYQYPTHLYNLDTINVDRFPLINIVERNEADAYVKVFFEKVHPYYFIFIESHFMDKYEIFWEELDSDVIKDTERSHTLSRLSSAEICTIYTVWILGRRFQQYADPENLPEDSVLDQDMIGKFIDIIKLSLSDVVLTPSIDGIRLLILFSVYLSSIKVRESGYCLMELAAIQAKSLGLHRKSIVNKFNDQKSDVMKRILWSLAKAETTLCCSFGRASAIPWEEIDIHFPVIEDETDEHFKIYYLQSCNLTKIIFEILDYKKKTQRDPLSLVSIEKALYFKKKLESFWSNLPESWKDYKSLPIKRYKPKLHIQYHYYYITLTLPMFLHIVNSHNYIVKNDDPFLSLLVCGIRSSFKTAEIVSYTDSKGFFNGTIYYDVFYCYNAIMVLTLTYILFKSSQPFKAKQIIDLNNLNSNYNINLEAILGSINLIRKLVLNNLHQIDGTMKRMSDIIETLLEDLGIIQTLVKKYNAPASNVKKIEGKDESGKTSVTSIPTGYRRLKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPP1</accession>
    <name>A0A1E3NPP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 10">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_15916</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 10"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019187.1">
        <property type="nucleotide sequence ID" value="XM_019161020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPP1"/>
    <dbReference type="GeneID" id="30177707"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF28">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="11">
        <location>
            <begin position="18"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="834" mass="95044" checksum="3E4D709AA673D16B" modified="2017-01-18" version="1">MPSNINGNKTGMPLSHTIKVVHSDQKWNEYDSSILEKFLESLSSDLSTEYVNIPEVVEKIHTGIPDEITTEELINLVAETLASMTTRHYQYSVVASRVLSHHLQKRLPQTFSANFTAMYKYDLASSNERKNTVISTEVYQFILENKDAIDSAIRPIRDFDIDYFGLRTLERSYFLKIDGKIHETPQYVFMRVALGIHFNDLHRALETYELISKKFFIHASPTLFNSGLESACFSSCFLVAMKDDSIDGIYKTLYDTAMISKSAGGIGLHLSNIRSSGSYIRGSNGVSSGLVPLLKVFESTAKYVDQGGNKRPGAFCIYLEPWHSDVFEFLDLRKNHGKEEMRARDLFLALWIPDLFMQKVERNLEWCLFSPDKCPGLSESYGDEFNMLYDKYEKEGHYMKKVKARELWHAILTAQTETGNPFLLYKDQCNKLSNQKNLGVIKSSNLCCEIVEYSSPEETAVCNLASVALPSFVENGTFNFLKLHEICKVVCRNLDRIIDINKYPLENCEKSNLKNRPIGLGVQGLADVFFMLRMPFGSPESKQLNIQIFETIYHATLERSCELAQKQGKYQSYDGSPISKGLLQFDMWKVTPSNLWDWSTLRQKIKQFGVRNSLLVALMPTASTSQILGFNECFEPITSNIYVRRVLSGEHQVVNKYLVDDLVKLGLWSIEMKNTIIQNDGSIQSIKAIPREIKALYKTVWEISQRTIIDMAADRAPFVDQSQSMNLFLQDVTFSKLTSMHFYAWKKGLKTGMYYLRTKAAASAIQFSLDPLKLKKPTINNKRISELEDEYDVKRSKRELSMETEGSTDSFDIFSTSVVSCNLEDPENCDSCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPU4</accession>
    <name>A0A1E3NPU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15905</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRP1/MDG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019175.1">
        <property type="nucleotide sequence ID" value="XM_019161015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPU4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPU4"/>
    <dbReference type="GeneID" id="30177702"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF81">
        <property type="entry name" value="CRUCIFORM DNA-RECOGNIZING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="4"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="242"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="316" mass="32855" checksum="19269667FA07B5E6" modified="2017-01-18" version="1">MPNFVFKWPADKKVKDVIVTGTFDNWSKTLPLVKRPDGSFELEVPLAKGTEKIEFKFVLNGDEWVTSDTYWTVVDETGNTNNYIEFAKDGSVVKAEKNMGKTEFGKTENGKTTQPAIIPESGLSMGSAAPAAGAGAAAGAAVLAHGKKEPEEVKPTVMPSQEGVQTTLGEPGVVVPENPQEISAFKEVRNVDAKKLNADIKEQEAAAAAAPGSAASSEGKAKYVKKVKKLVSPEEANKAEADASAGATADAKETPASAGTASTTATGTASTAASKDKGKSPAKKDTAPSTAASSKTASSAKKEKRGFFSRVKKALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ00</accession>
    <name>A0A1E3NQ00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_31019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018736.1">
        <property type="nucleotide sequence ID" value="XM_019162669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ00"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ00"/>
    <dbReference type="GeneID" id="30179356"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="44892" checksum="A7FA9C5656DB44FB" modified="2017-01-18" version="1">MNTQKSLQTSALSKEPKLLNVAFNQDQSCFAICSEKGFQVFSTYPMEMKMERDFTLTGRRLTHDEETGSGHWKEADTAVKSSTTGIGIIKMLYKTNYVALVGGGKKPRFPLNKLCIWDDLKEKNSIILEFTTPILNVHLSRTHIIVLLKNMVLVYSFKLKPDLIFSFDTIDNYSGVSDHAITENSSILVFPCRNSGQLHIVDILNKKKNSSEGDEDKKTVSLIKAHKNPIQCVCLSPSGKMVASASDIGTIIRVHDTRSCALLYEFRRGLDNAIITDMKFSPNGMKLAVLSDKNTLHIYHVYGDEVNGKNKTHILKDLPLFPNYFKSTWSFVSKDVGNKNDVINDVGTLGWADDESVIIIWKFRGIWEKYDIIINNSIEHNKGSRWQIVKEGWRSVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ73</accession>
    <name>A0A1E3NQ73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase D1</fullName>
            <ecNumber evidence="3">3.1.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Choline phosphatase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphatidylcholine-hydrolyzing phospholipase D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_16026</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019328.1">
        <property type="nucleotide sequence ID" value="XM_019161062.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NQ73"/>
    <dbReference type="GeneID" id="30177749"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="936"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="1296"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="433"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="531"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="714"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="990"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1652"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="461"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="536"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="740"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="990"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1008"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1660"/>
            <end position="1704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1930" mass="221454" checksum="D4E7D694B966669C" modified="2017-01-18" version="1">MTTPVLSKITSSGSANSKRIPSNHTDTSSMKMATRTNSSASNVSSQYTDALQSVPSNSQDTRDFVMAKRIKESEVVGNKSNLNPLQAPFAYLDGSRTLGEEVQVVASGIKNMLHRRHMMLDDSGTKESQDKERMASIERKIHQLEDNDKNTGQPDIDELVGPDGTISPDIVRKSLEKEKENRIIRDVQHSTEPITLPNRNNTWQHSNHEQYLENSKLRLIRSENSSLEESPNQNLTVEDTTANLRNAIKPHDLSNDPKYILDAKNDKKTALMVDAFLLGYADMLYLTSSFFKDENGIRKGPLMIPLLSLYLTDITVEVFLSRKINLNHDNHITSSLNKEDLLKLATFTKNRIFRLHLEYGVGDHVLRWDMEKDTKALVVLHKKLLRRTKERLDKVKTKEAEIPRFPVLPSHFAKFRGLFKGDREILIANDNKSEKKKSLNSEGASYRNVHSNKTSIAKGYNPKEKNSSSKSEHSNVDRAPTIDNQIGSSQSVRTPPLQRLNSASSQNSREESLHSLSNTLSRFSTAAFANLRSYNKPHERNRDKDKEKVDKDKDKEKDKERQKEREKEKEKNKERERHLIALQERTRLNQKYRNDMQLYFDSLLENVSFKEQSNKVLQFFELSPFSLLLSNEIYRKRKEGYMFIVSSASKQGWRVGHFKFHDLSEMIKRHTSKWCILGDSFLIYTSDISSTTPEEVFLLDPKIQMSVKGFSEKKNNYGGTEDSDSDSENYYNYNINDNINENENENENENENDDVDDYYFANSKSVLKSAKSYPTIKLTNSERSIQLVAKNPRITRQWASCIRSIISETEWAKPHRFGSFAPVRHHAFAQWFVDARDYWYAASSAIEMAKDVIYIHDWWLSPELYLRRPANGNQNWRIDRLLEKKAKQGVKIFVIIYRNVGNTVVTDSMYTKHSLLDLHENIYVLRSPNQIMQNVFFWAHHEKLLIIDHSVCFLGGIDLCFGRYDTPDHVLVDDSKYAFKVESMNGNLSSSLTANTSSTPNTKRNRSSSKTPESGVTDSTSLEHHLADSNLTDSKWSAEYDFQEQFQVFPGKDYSNPRKKDFFELTSPHEDMYDRQTVPRMPWHDVHMVTGGQVARDLSRHFIQRWNYLLRQKRPSRPTPLLVPPRPFTDEELSKLNLKGNCEVQLLRSSGDWSLGLEEHEQSIQNAYIKCIEQSEHFVYMENQFFVTSCTVESCVVKNQIGDALVDRIITAHKNGEVWKAVIVIPLMPGFEADVDTKEGGSVRLIMQCQYMSISMGETSIFARLRRVGIKPEDYINFYSLRKWGLISEKKLLTTEQLYIHAKTMVVDDRIAIIGSANVNERSMRGTRDSEVCAIVRDKDLIESKMDGKAYHVGKFSHSLRIRLMREHLGVDVDLMDLVERRFAEIEKFCNTELGNNASVLKANEGKTSLSAMVEMGTRYLLGLNGGTPGFCKRYGEKNHKFGEKLTEEMIKTFTCIKTEQENTDSVDDIEFSFSFNHRAGNENAGIRSDKTLSSDTRVTTENHRNEVRGDVDGFNSKYYEEAKAQMKKFLAQRVNQCAQSVDIEKREAHDKHYPLPVFEDIVDVLSQHIVQNASSGEQNAINTDRWTMVKRLYYMKKLYCKLAANAHRENKSSSEIFEDAEDSYVRSRSNTNPEMLKSSFKNADVHLSNDSNGDAIAGSDTSKSASHPLDTASSTANRSHMNGGDTNRLTSTPAEGKTNNTSAKKLRLPDIPLIKLKNNDINDINKNMLPQLDFDFLDPYAFEDPLDISFYEGTWMTQAIRNTLLFQMVFHVQPDDLVQTWKDYKDFEELKNAFETYQRIYRDGVINGEGNNVFGGEEAQQEGFDRESRGSMGHPLDDDVVEHRRRLERSFKLAELERFQRTSGPVGTTLPGASGYGRGAVYDYPTSLRLMRMVRGHVVMFPTKWLKKEVEGSNWFYKADKIPPIQIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQI6</accession>
    <name>A0A1E3NQI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Halotolerance protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_30734</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018916.1">
        <property type="nucleotide sequence ID" value="XM_019162652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQI6"/>
    <dbReference type="STRING" id="763406.A0A1E3NQI6"/>
    <dbReference type="GeneID" id="30179339"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045807">
        <property type="term" value="P:positive regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903329">
        <property type="term" value="P:regulation of iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13994">
        <property type="entry name" value="STKc_HAL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000183">
        <property type="entry name" value="Serine/threonine-protein kinase hal4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF558">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="131"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ47803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="407" mass="46719" checksum="37CA810A90B4BDD7" modified="2017-01-18" version="1">MRFKILPDGSHQHHLARVKRQEKLGQMVKDWLGAGKKRSVAVSAVPNIFSSSNLNEMDSQIKAALNGENVSAAGAKLPPTLFSMYLREQKMNAHSPTAYAQPSFSVPAAGKAHEHDHDHDHDHESFFEKYGKCQEIIGRGTFGIVRVSHKKVNNREVLYAVKEFTRKLNEPDSKYSKRLTYEFCISSSLKHDGIINAYDLFKDSKGDYYEVMEYCSGGDLYSLIAAAGKLEYSEADCFFKQIMRAVHYMHNMGVSHRDLKPENILLTQDGRVKITDFGSAECFKTAWEDDIELSNDIKGSSPYIAPEQYTSTEFDPRFVDVWSCGVIYMAMRTGRQLWKEAQVDDEFFHEYLRKRKCEKGYEPIEMLKRARCRNVIYSILDPVPERRITTLQVLNSEWVREINCCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQP7</accession>
    <name>A0A1E3NQP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019543.1">
        <property type="nucleotide sequence ID" value="XM_019160180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQP7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQP7"/>
    <dbReference type="GeneID" id="30176867"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="688"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="476"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="717" mass="78767" checksum="C2961F1AD4D0B4D6" modified="2017-01-18" version="1">MITGYTFDTPSSSGTITELENAKIAKKSTGKRKFHKKSRKGCATCKRRRVKCDEARPLCGNCERMGIQCIFLSAEQHGNNEMYEHEHDQNDSSEHVYVKQEAEHSPSFPKKEPQLDIPKNILPATPFMMNESLANMSPVSPISQFLHQIPQSPAATAVASQFLNNFPPEVQMKLFQQFQQLPPEKQQQLYQSLPMSVKSSSESLTMPEESILSNISRTAGGNPLSDLLGDIVPNRNNFLKQSNTSKTPTPQELAAQYQQRSQASKDIPILAENGGPSLSPDSIRSPLPPITNGQSTTTGSISDLNMLDLRLMYHYTTKVWPTITAAGISDSKIWSDDIPMLAFKYPFLMHSVLAFSATHLSRTERGLDQCVTCHRAEALRLLREAVLEMSPENTDALVASALILIMDSLANASLSTSSSIKSLPPSAWIFHVKGAATILTAVWPLSEKSRFYKFISVDLGDLGEIGYDLISGELSNTSDSNENSRSSSVSQEKGTSKAQPSNTSSGHLGTTSNKRMRVSSPPITTNLAGYISGGGNKSKKPRFSSIECFDNEISDMFPISFNSPYFPTLAYMARLHNERDKSDFILRIFAFPALLDKRFLGLLINCDLSAMRIMRCYYKLLRNFTEEMKEKVWFLEGVSSVLPVDVEQYAGGGGMHMMLDFLGGPSTIDDDGNGENYVAKSAAQSGLLDTNNLPSASMTGSLGLDDTSGLNNDGNDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQS0</accession>
    <name>A0A1E3NQS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019557.1">
        <property type="nucleotide sequence ID" value="XM_019163542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQS0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQS0"/>
    <dbReference type="GeneID" id="30180229"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="924"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="759"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="762"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="789"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="818"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1135" mass="127217" checksum="200E63D6C131CCBE" modified="2017-01-18" version="1">MADLDGLNITFSNGDVNPPNHTVFSNTLHNDSQSTINSTRNQHEVSLANLQSENTMLHKKVEYLQKIIKLKDNTIDDLNKKLISLSHHSREGSTATNSSSATKTSHDVVSTESLESDDNKEPKELAESSFTGDAITLNSPISHRQQSQTNHLGTASVDSASSQTIWEIPQRNSNRTASSHSNLSSSKSSAHGSDNSITPQHITHFYSQSRSATHTPTSSGHAIRKSVSSTTSSHYSNDDEGEKKFEGYELGNDYENTNGLSSHDNTVADSSNNLTLDLQNQLHYADMTLGSSPRKLSPKRRPQASGNAINAKDLIAEGNHNDNDNDNDDDDDDLHFGIVSTPEEPVYKKLGQDSLYSNSSTALDYLPLKTPVNCNFDSSITDLPLATPVSEYSVSSSSISGTPNNMHLDSTFLHPATSYQNGKQERVALVPPTLPPHPPPPPHHPSNENLHFSRSQPRSRSQSQLQSQSESQPQSQSQSESLQPQKQIVQKAVEPTDDSEKFLLNSLKHCVIRIESLIDPQGLAVPIDTITNHVIAKSKRDNYKVSFLVYTDENPSQLTPIYRVKRSYVELLIMDKTVRPLIPSLPKLPDFQHVASLNCKFWAHSKKVIQNYINKLLTLLKSQNPLDQDNPIWKHFSNYFEFKMDPNNLEDPEYSVLNLQDKICYLLYIRKNFTMKTYDFAKLSSQPETNELVINFLLTKSQEVLLFDEVGIFFKGQEVTIKRKKKFSANKSWSFFAESEYDASELCSKVNAWIESYSGPHASDETEEKATAEGRTKHNSEESDHKSSSNDNMLSPSINTPWKIFKKSSKQIPPSPKPNHNNNSNNHNNSTSNSSSHSLPSTPSKMISSNSSTPSNNHNINDVLNFKAMPYESNKLTSESNNNSSSNLMPISPLRHGSDKPVEALFQPIDETPKYFKSTLKYSFDLCPKYKLYGLSVPSIVFQSITFLYQQSGEGFEGIFRLNGMMSEVNKIQEIFNNQYDCELAKLSPIPDVHSIATLLKRYLRNLKDRLLSDEVTSELCRIINNNDNHLKREQDKTEDEAEREVSGLILSPTTLLSPSSSEEFKQIYKYKVPQLNRNVLYALFKYLRDVLKMGKHNKMTVSAMSVLMGPNLTQSDGGGQICTVLLENFNQIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQU7</accession>
    <name>A0A1E3NQU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_28965</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019539.1">
        <property type="nucleotide sequence ID" value="XM_019162566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQU7"/>
    <dbReference type="STRING" id="763406.A0A1E3NQU7"/>
    <dbReference type="GeneID" id="30179253"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034737">
        <property type="term" value="F:ergosterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF23">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="441" mass="52148" checksum="9260B600929BDCF6" modified="2017-01-18" version="1">MRSNFGDLNFIPRETIFDSEIHFGAKFYGLYIAFWIAVAFFGVDVVSGYHQSHGSLLNSEIFKLMMKDLWKVALTDLAMYLSMYTSVVIQVLVKHNIIRWKYTGFVLQTVYEVALLYCPLSFADRMDYPWIAQIFIMLHTVVMLMKVHSYAFFNGYLWEITKELRFSAAFLKKREFLPENIKKALSNSVEFCNSEISSQEFPSNISFFNFFEYSFFPVLVYQSKYPRNEKIRWGYLFTKIAGIFGVIILMIAIAQHRLYPIVSYCLEIQKTTSLSHRLKEYPFILIQTIPPFLTIYLLVFYLIWELILNAIAELSRFADREFYSYWWNSVDWNEYARDWNVPVHKFLLRHVYHSSISAFKVNKTAATFFTFLLSSVIHELAMYVIFKKVRFYLLCLQMSQLSLVQLSKTKWMKGKYVLGNCIFWFGVVFGPSLMCTMYLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR87</accession>
    <name>A0A1E3NR87_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase III subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_56831</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RNA polymerase III (Pol III) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPC7 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019718.1">
        <property type="nucleotide sequence ID" value="XM_019163201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR87"/>
    <dbReference type="STRING" id="763406.A0A1E3NR87"/>
    <dbReference type="GeneID" id="30179888"/>
    <dbReference type="OrthoDB" id="5377312at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024661">
        <property type="entry name" value="RNA_pol_III_Rpc31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11705">
        <property type="entry name" value="RNA_pol_3_Rpc31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000777">
        <property type="entry name" value="RNA_polIII_C31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="233" mass="26678" checksum="3D81C6575C924F7F" modified="2017-01-18" version="1">MSFRDRGQRTILPFGLDWADIQQNATETLEFPVPVNGKPTRIETECFQQFSNLTTLMKDSSFYTGNLKSLLLDEVQNGGRKKASIVYLEGGVNDGLKRYSDKYRKKIKIGKSITDHPFILRFFPQELHSVMSSKSGKKSLNVSKYKQGSKMGMNIDKIIKTAEERQQEILQRLNEVATNNNDDDIDDADNQEEEENFDDEFEDDEDDDYNAEKYFDDGDDFGDGDEGDEEAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRL0</accession>
    <name>A0A1E3NRL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_61191</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019835.1">
        <property type="nucleotide sequence ID" value="XM_019163461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRL0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRL0"/>
    <dbReference type="GeneID" id="30180148"/>
    <dbReference type="OrthoDB" id="61815at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="8">
        <location>
            <begin position="189"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="694"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="701"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="716" mass="82347" checksum="9F5B402CDEFF1FD2" modified="2017-01-18" version="1">MPPKNVETRKWKPPKGPKNTPHKNKNTIGLGRTIKNAKALQNQVYYLPDGQMRFTTDKHEADWVKLRSVTQENALDEFLNTAQLADTDFTADKHSSVKIIRVNNQAVDTGSYNPYLLTSAEEANKFKVQKEHIKDLIVPRRPAWDSNTTKFELERMESDAFLEWRRRLAVLQEDHDLLLTPFERNIMVWKQLWRVVERSDLVVQIVDARNPLLFRSTDLEKYVKELNPLKRNLLLVNKADLLTYQQRLTWANYFKENGIKYTFFSAAKANEILEKQKEEEEALKRYADEHEGELPPVYRSQEKTESDEESENELDISELSEEQRAEFLKLQKELDELEELENEMQDYDIDDEDEDRDEDEEHHKVEENYFDEEDEPEDADLREMTRIITVEELEELFLRVAPEPLSVPPEGQPKRLQIGLVGYPNVGKSSTINALVGSKKVSVSSTPGKTKHFQTILLSDEVILCDCPGLVFPNFAYTNGELVCNGVLPIDQLREYTGPCQLVCQRIPKFFIEAVYGITIPVLSETEGGTGIPTAQELLTAYARARGYMTQGFGSADESRAARYVLKDLVSGKLLYVEPPPHDDGSKATESECREFNKELYNISHLPEQRRNQLIQAINAKGLSMKNFDLAKDLDKVTFSGHLSVEDESNKHRSRTYGGKQAALYSASNELDDEFFHMKSVEGKLTGPFYRNKATTNQEKKHNKANRKQKTRRLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRM3</accession>
    <name>A0A1E3NRM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14254</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019837.1">
        <property type="nucleotide sequence ID" value="XM_019160293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRM3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRM3"/>
    <dbReference type="GeneID" id="30176980"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="73"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="432" mass="49706" checksum="70F27A3D2C5A1725" modified="2017-01-18" version="1">MASSVPSNLSMNMNSNNTNTNMNMNMNVNVNSGGVGVGNSSSELLGRRQSVPMETVAEEVFDGKTEQPYKLLFSQRSMVGHGSFGYVYQINILPANKKAAIKTVLQDKRFKNRELEIMRLIHHKNVVNLIAYYYKTNDKDQLYLHLVLEYVPETLHTASHWYFSKHKSMPLLAIKLYSYQLLRSLNYIHSLGICHRDIKPQNLLIDPISGVLKLCDFGSAKILKPSEPNVSYICSRYYRAPELIFGARNYTSKIDVWSAGCVIAEMVLGQPLFPGQSGIDQLVEIIKLLGTPSKEEIRSMNPNYMEHKFPSVRPIPLIKIFPNVEPEMVTMFVKIFNYSPAKRITAAQAMYEPYFNLFREKSFQNTQMPNFRGYKLINETTVPDLLDFSKRELSFEPELWDKLLPEWKRQELDMHSFVPYSVQELQDTDNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRP7</accession>
    <name>A0A1E3NRP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPIase cyclophilin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_9285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019875.1">
        <property type="nucleotide sequence ID" value="XM_019165084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRP7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRP7"/>
    <dbReference type="GeneID" id="30181771"/>
    <dbReference type="OrthoDB" id="442970at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="34"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="246" mass="27185" checksum="0F9C79A130340F13" modified="2017-01-18" version="1">MPRYFVEFVNLQRKNGGKVSESPETQCPISFTATVKLFSNTLPNTTRAVAKLFSGDGSDGKDSTEPITYRKSQVTRVVAPEYLVQFGEPVRRSQAGVQKLGPLVDAEEFRRCEAKYAGKIRGRRGLVCLAAKDGEAADEADTRSVQLCIVFVPWGEYPELDGYVVVGECKEWAELRRWMAAVDVKENPATGSWEIPTGSGVWVNRCGQILDKPAPHGASSRLEEPGAKRPHRVKAVDLLFKRSRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRR2</accession>
    <name>A0A1E3NRR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_47403</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019491.1">
        <property type="nucleotide sequence ID" value="XM_019163129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRR2"/>
    <dbReference type="GeneID" id="30179816"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="510" mass="58937" checksum="82F5E18E8E729A3D" modified="2017-01-18" version="1">MSEKIRSTQAENQEDSGSDLDSQLNESKVDLLEANGSRVLKKRSSKGCITCKIRKKRCDEIKPICGDCKRLNRKCAYITSEMSSDEKKVLKEEMKLIEKNSKARHRKKKQQNQNRILSIIKSNTQPQIYTNPSIVLNTTLAGLTPIGKRLYEYYRDKLSFIVCSAPKVENMYLNTFLPMAHIDKSVLYGILAWSAFHLGGPKMEKQGGDDVEIYEDLELSRLNRDNMINMRLAAFLILCGVEICKGDVSKWSKYLTYGAKLIKLKGGLERFNESKDEHFLVTNYAYHDITAIQVINERTIHFDLNEYENMWTKSNELGFADPLHGISSPVFKILAEINKLVIVVQKLIKRSMKDETKTPENIEQRIAGVKPKIALCVSNRDLELQLTMFECFQVTAKIHLRQSVLRMNSASIEIQYLTTQLVKLLDVLLESDVEACLCFPMFIAGMNCVWKKDREGMTRRFKSFINRYKWKNVLRCQIVIRYIWELNHKGEKFVDWYAVVKTLGWDLSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRR4</accession>
    <name>A0A1E3NRR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70391</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019895.1">
        <property type="nucleotide sequence ID" value="XM_019163628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRR4"/>
    <dbReference type="STRING" id="763406.A0A1E3NRR4"/>
    <dbReference type="GeneID" id="30180315"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027323" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="548" mass="61499" checksum="A8E635D89D8435E4" modified="2017-01-18" version="1">MKLALSAAAVVSLLVSTASANPVLGYNKQNVLSFKDKQQHRHPILDKYLKEGLFDQGLVDEKISEAWELLEETVDASKIEEELKRYSSEEFKAPKSALRQPELKDTANYEHINLAEFSSLYGLRVKKHVESNPSVLGVDTVEQWTGYFDINEEDKHLFFWFFESRNDPENDPVILWLNGGPGCSSMTGLFFELGPSSINGTTLTPVSNPYSWNSNASVIFLEQPVGVGYSYAEKSTVSSTKQAAEDVYAFLQLFFAKFITFASNDFHIAGESYAGHYIPNIASVIANKEDKFFSLTSVMIGNGITDALIQYYYYIPMACNATESGYKQLIPDDECEDLEKMYPRCAALINTCYRSQTALTCLPANLYCERMMTPFEKTGLNYYDIRKPCLGDGCYPQMSAIDEYLNKPEVMSALGSEVKSYVGCDNQVFRNFILSGDEPKPFQQFVAEILELNIPVLIYAGDKDYICNWLGNQGWSNELSWSGKESFNAANTTNWYSKANGSHAGTAKSNGLLTFLRVFDAGHMVPFDQPSHSLDMVNRWISGDYLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRT8</accession>
    <name>A0A1E3NRT8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_87175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019913.1">
        <property type="nucleotide sequence ID" value="XM_019165023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRT8"/>
    <dbReference type="GeneID" id="30181710"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="71" mass="7669" checksum="936899706EB8ECE2" modified="2017-01-18" version="1">MPGKSRKTLHRRLAVAAPTRSSTVKLLRYPRVTCVMLAAAYAEKMDCGSAGITVQNCRRTDVSTPCGKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRT9</accession>
    <name>A0A1E3NRT9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="12">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="4 12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_13984</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. SSU72 is required for 3'-end formation of snoRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="1 12">Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the cleavage and polyadenylation factor (CPF) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4 12"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019521.1">
        <property type="nucleotide sequence ID" value="XM_019160164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRT9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRT9"/>
    <dbReference type="GeneID" id="30176851"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180007">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032215">
        <property type="term" value="P:positive regulation of telomere maintenance via semi-conservative replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090052">
        <property type="term" value="P:regulation of pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902801">
        <property type="term" value="P:regulation of siRNA-independent facultative heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009302">
        <property type="term" value="P:sno(s)RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030847">
        <property type="term" value="P:termination of RNA polymerase II transcription, exosome-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030846">
        <property type="term" value="P:termination of RNA polymerase II transcription, poly(A)-coupled"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="236" mass="27335" checksum="79EEF052B630D9FE" modified="2017-01-18" version="1">MEDVPNLRFCTVCASNNNRSMEAHKVLKEAGYQVRSYGTGSKVRLPGPSINKPNVFQFGTPYTEILKVLESQGIQVHKNNGVLDMTLRNMNIKKSPERWPYFNQPMQKLPEEEHPGFQDELDFEIVITCEERCFDSVIEDFLARNYFSPNELDRKVHVFNVEIRDDRENAHIGGQAILELANSLNQLFLKSKGAMEGDNADDPAHPEYWEDNIPDLITQWQQKHEMFPLLYSLCYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSL0</accession>
    <name>A0A1E3NSL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_90644</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020147.1">
        <property type="nucleotide sequence ID" value="XM_019165058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSL0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSL0"/>
    <dbReference type="GeneID" id="30181745"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062040">
        <property type="term" value="C:fungal biofilm matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="608"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="615"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="69972" checksum="8B142FAA8E074DA2" modified="2017-01-18" version="1">MSKAVGIDLGTTYSCVAHFANDRVEIIANDQGNRTTPSFVAFTDTERLIGEAAKNQAAMNPQNTVFDAKRLIGRKFDDTEVQTDMKHFPFKVINEGGKPKIQVEYKGETKVFTPEEISSMVLTKMRETAEAFLGTKVNDAVVTVPAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLDKKDSGKGEHNILIFDLGGGTFDVSLLSIDEGIFEVKATAGDTHLGGEDFDNRLVNFFATEFKRKYKKDLTSNQRALRRLRTACERAKRTLSSSAQTSIEIDSLFEGQDFYSSITRARFEELCADLFRSTLDPVEKVLKDSKLDKSSIEEIVLVGGSTRIPKVQKLVSDFFNGKEPNKSINPDEAVAYGAAVQAAILTGDQSSKTQDLLLLDVTPLSLGIETAGGVMTKLIPRNSTIPCKKSETFSTYADNQPGVLIQVFEGERAKTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSALEKGTGKTQKITITNDKGRLSKEDIERMVSEAEKFKEEDEKESKRIAAKNQLESYAYSLKNSANEAQFEEKVGAEDKEKLTKKCEEVIEWLDSNQTASTEEFEDRLKELEQEANPIMQKLYAAGGAPGGAPGGAPGGFPGADAGPSVEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSN4</accession>
    <name>A0A1E3NSN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Alkaline phosphatase</fullName>
            <ecNumber evidence="2 11">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_14599</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="2 11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020224.1">
        <property type="nucleotide sequence ID" value="XM_019160452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSN4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSN4"/>
    <dbReference type="GeneID" id="30177139"/>
    <dbReference type="OrthoDB" id="7392499at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047386">
        <property type="term" value="F:fructose-2,6-bisphosphate 6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000063">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133498" description="Alkaline phosphatase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="570"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="578"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="8">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="506"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="599" mass="66411" checksum="48210E16C11711DB" modified="2017-01-18" version="1">MALLSILSLLVLTYYVKDVKADGFGETSTSREVNSFFGEEDMNGQSPLYFGTQDVVAPQKSEKSEKKKSKNKNKKNIIMMVTDGTGPSSINMARSYNQYINNLNFDDILMLDKYLIGQSRTMSSSSLITDSAAGATAFSCGLKSYNGAIGVDPMQRPCGTILEALKLKGYKTGMVVTTSLTDATPAAFSSHADFRSMQDLIANQQLGLNATLGEMVDLMIGGGRCFFLPQSDAAGCRSDELNLVDFAKKQGWEVSVDRAGFDKLGLGLNKDVKLPVLSLLAYYNIPYEIDRDEKVFPSLYEETVTALNILSRETEDSDEGFFLMVEGSRIDHAGHHNDPHAQVREVLAYDKAFAEVIRFAQNSDVETYIVSTSDHETGGLDVGRQIGPDYPDYVWYPEVLKNAKHSGEYLNTKINRYLSLNKKIKRDELKSFIQKEILSEDLGIADFEEGEVKNILKMIVEEESEAEAGSDGFVTIKEVTGASAVVEYLKNMVSVRARIGWSTHGHTGVDVNIYGYCNRDEGMVRLLKGLGGSRENTDIGRFLEQVTGVDLDDLSDVLRRRGQVHNYLGDESERRSEEEIEEEIDAAETDNEGFINVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSP9</accession>
    <name>A0A1E3NSP9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_57247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020278.1">
        <property type="nucleotide sequence ID" value="XM_019163223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSP9"/>
    <dbReference type="GeneID" id="30179910"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="53" mass="6149" checksum="988A8F4515FCA4EF" modified="2017-01-18" version="1">MYGSAWLRPMVQMKPSPYVFYAAVQFYAAVDGASRTRHDELRAYHMKTVKPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT64</accession>
    <name>A0A1E3NT64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_99015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020404.1">
        <property type="nucleotide sequence ID" value="XM_019165141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT64"/>
    <dbReference type="GeneID" id="30181828"/>
    <dbReference type="OrthoDB" id="3987294at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="109" mass="12609" checksum="D9532B6E7C53CB0A" modified="2017-01-18" version="1">MIRALCKANLHEGQMRKQAVGIKGGLVRQLHVSSRKGQVNEKKSKLDGATDRKYYYEMPGNQLNPHSYFYRVLAVPYMKFCGVIIATYYGMSGLWEYLDSRDRRKSDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT69</accession>
    <name>A0A1E3NT69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="7 19">1.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_14679</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 19">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="19">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 19">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 19">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="7 19"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020319.1">
        <property type="nucleotide sequence ID" value="XM_019160483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT69"/>
    <dbReference type="STRING" id="763406.A0A1E3NT69"/>
    <dbReference type="GeneID" id="30177170"/>
    <dbReference type="OrthoDB" id="419183at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979:SF1">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16 19" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 19" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 19" id="KW-0520">NAD</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="20">
        <location>
            <begin position="6"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ49206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="364" mass="38491" checksum="BB65F04321A8A870" modified="2017-01-18" version="1">MSTTKNIVLLPGDHIGTEVVAEAVKVLKAISEVRPEVEFKFEKHLIGGAAIDATGVPLPEEALAAAKKADAVLLGAVGGPKWGTGSVRPEQGLLQIRKALNLYANLRPCHFASDKLLGLSPLRSEIVKGTDILIVRELVGGIYFGEREEDDGSGKAWDTEAYSVPEVQRITRMAAFLALQSDPPLPIWSVDKANVLASSRLWRKTVEKTIREEFPQLTVQNQLVDSAAMILVKSPTTLNGVVITSNMFGDILSDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPDLPANKANPVATVLSAAMMLKLSLDLVPEGKALEQAVKNVLDAGIMTGDLGGHAGTSEVGDAIAREVKKLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT78</accession>
    <name>A0A1E3NT78_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020458.1">
        <property type="nucleotide sequence ID" value="XM_019160545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT78"/>
    <dbReference type="GeneID" id="30177232"/>
    <dbReference type="OrthoDB" id="3993103at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="35467" checksum="A78B1F4596461AA7" modified="2017-01-18" version="1">MQRRVSLIVTEQGPDRNSAAGSPNDAFRKQQRSPHPSMRAFEENDKRPSESLQGSSMGHTRSMWCTSDMNNGSQSQQTADEGLHSRRVSLDDLDVASLRPRGGEGSGSVPMIGSLVYYRHKSDSAILNQNTQGDAQGNAQGDEHGAAVPRPATLTTAPPARPCISDDGDGADDEEALLSDAESVASPIPPHMRHRKYRHFSYPWIYADEADEFGMINSKIDSRIRQNDQRLSSYFQNQRKRSHFNNTHYHIPGLDPAPSPDSELSESSAPAALPSKSRSSSTSMRDSVSPNSTGNRSPHTNSRHRRRLTCAMIFTTPKVVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTE6</accession>
    <name>A0A1E3NTE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_98967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020399.1">
        <property type="nucleotide sequence ID" value="XM_019165140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTE6"/>
    <dbReference type="GeneID" id="30181827"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="133" mass="14647" checksum="B8DF330A104D6FE6" modified="2017-01-18" version="1">MRHIISLHHVLVAVWHITYAYVIAYVIWLYLSSPFLLLANAGPICVLDTSARQHSQSQRSKTSGQCRGLRQWTGRAKTATGSSRMLRGQKIFFSPAAAREGTCSPFRAGQSLFLLGLLTTATLSVSSQLQAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTI8</accession>
    <name>A0A1E3NTI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020544.1">
        <property type="nucleotide sequence ID" value="XM_019160583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTI8"/>
    <dbReference type="GeneID" id="30177270"/>
    <dbReference type="OrthoDB" id="2444812at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="191" mass="20257" checksum="58E39D9220FCF744" modified="2017-01-18" version="1">MGVKNSKPEVPKGWVAVWDEKYNEWFYVNESTGKSQWEAPKGTVFGGNESSPPSYNQATQAPQQQNSQTPGADRQYASQQQYVPQQSFAPQQPFAPQQPFAPQQQYVQQPYYQPVQQQPARSRFGGLGGGMGGMGGMLGGMALGTVGGLLIGDAIGHDDYGQGYGDYGGDYGGGDFGGGDFGGGDFGGGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTM2</accession>
    <name>A0A1E3NTM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair protein REV1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Reversionless protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_70885</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020449.1">
        <property type="nucleotide sequence ID" value="XM_019163761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTM2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTM2"/>
    <dbReference type="GeneID" id="30180448"/>
    <dbReference type="OrthoDB" id="427711at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017125">
        <property type="term" value="F:deoxycytidyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17719">
        <property type="entry name" value="BRCT_Rev1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000001">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000011">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21999">
        <property type="entry name" value="IMS_HHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="17">
        <location>
            <begin position="238"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="UmuC" evidence="18">
        <location>
            <begin position="451"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR036573-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ49336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1075" mass="121687" checksum="61F245F71A9F3635" modified="2017-01-18" version="1">MEDGEPTPESEKFKNFTESAHESSWGSLHDSELNIALRQQFSKNNSKKDTSNIEIATDVDTDEDILHYSNNNGRNAVATDIKPPTLPNYAKESVSDDWGSMNDSQFISFVNKISQTQRDSKSTASVKKTLLSSDKANNTAESSVFLDEDFQISSTPKCKVSNISSSPILLAQKLPQLREKGTEVEYQEDVMTKQDYEDSNRYAFEDHEGYFKVKNTKQRKQDQEYSEFLKGKKEDGQTFPPLFSNCAIHINGRTDPDILQLRKMILLHGGTNVPYLSSKGIATHIVAESLPPRKRIQFSNCKVVSPKWIVESIAAKKLLNWAQYRLEQLGEYGQQEINFLKVNTLMENNDVSEVQTGNIADHGTDMEVNDSLSEDKRAEKEDILSQELSRAGVDARDSNFLRIFFSKSRLHHLSTWKADLRSEFLNKAAALLKERQFDAPKNNIVPENRVILHVDFDCFFARVSANLHQPMIDFDNTPCCVTHGGASADVSSCNYVARQFGVKNGMWFSKAQKLCPNIVCLPYQFDDYERVSNIFYAKLLALNIDSILPVSVDEALVDISSLCMESGGDTMGVLKNIKCELDTATGCTVSCGSGKNVLLAKLALRKAKPNGVYVVPSDENQILRFLDDIDVRKLPGFGDRLFEKLLSLMGKGTNTTVTISSLRCIEKEKLISSFGIKTGEKLYLFSRGVDDTSIDLLSDPEKYLRKSLGIDVNWGIRFSSNAEVEDFLNRLAAELSKRLVDCCMTGSMLTLKLSIRHPDAPIEPPKYLGMGYCTFVSKSSRLGISTRTTGILSSEMKYLWRFLNVDPKELRGVGVSMNKLIPEGNGKIDTNNQMKLQFKPAAATSLHTAIQKELPEEVFEDPPALEPPAGIGHKIQTHQNFSPSKRTRGDDFLSEEIDWEVFDNLPASLQEEIKRELRKRKLQSSPIRKKVASTGKDIAFMLSPSKRTPNNIQQHDIYNLITPEKVNTKDENQIIFQGINVSEEPKILKKLLFWMDFTLEEKTGIEKKDLELFKDFMLKLLSRNDVIRYTRIVSAMSTYLHLKKDMRGYEEWFYQLKQLKALPDEQSFTAFEFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTY3</accession>
    <name>A0A1E3NTY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020655.1">
        <property type="nucleotide sequence ID" value="XM_019160617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTY3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTY3"/>
    <dbReference type="GeneID" id="30177304"/>
    <dbReference type="OrthoDB" id="42889at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF147">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="68293" checksum="D4C11BFD0086CB2E" modified="2017-01-18" version="1">MTEKLQALLEWARSNGLYVNEKLTFKDTAEHGTSCIIAQPLEEQDKTGLIKVPKELLITPILADAFASKYLKGVNTSTSKNANSNLLFLLAKLKFDKTNRTVFQESNVDLSEQFKPYLDYLPDTGKALGNPYFWTMEEKEMLDGTDAYTFMKRDFLKDLEDWKKIVLQMDIAEHPQLKDELLEYEAFKMGPVGGVAVDYLLNIKEVSWTSFTAYLWASCVVSSRAFPYILFDQSSQYKSQAFLLPIVDLLNSADDNQSKCRWTIEDNQFVFSTLDDLSKLRKNCELYNNYGDKSNLSFLLHYGFCLKNNDNESTTLTLKLDPSVIEGAKNYGVIIPKDSTEEVINFELRKNSPLSKDLINFFAYLVKLRSEKTGFTVRMKLEGSTQLRSIIKTKLKTLKKVDVVMSENVSEVNAKNIKLYRKSQKDIFQKYLEQIEQFEKQLLTEFKPFSFKKALQADKAFFNSFLLVFGTRTYNDLIEKGILDHAVLLWIMRIANKNIYTDIPDTSIFPEFIYNEFEKVKNSTEIDSEDIAEYLPMYQSLFPALCEKIPIVYNHGDWTLNNLIYAGTVADRLTYKRETNGEVFFIDPSRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU23</accession>
    <name>A0A1E3NU23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020697.1">
        <property type="nucleotide sequence ID" value="XM_019160635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU23"/>
    <dbReference type="STRING" id="763406.A0A1E3NU23"/>
    <dbReference type="GeneID" id="30177322"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805:SF1">
        <property type="entry name" value="GP-PDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="4"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="320" mass="36979" checksum="C3BC866683997770" modified="2017-01-18" version="1">MSAIILAGHRGYKALYPENTICAFQAAVDAGCDVIELDLHTSIDGFVVVTHDVSTHRVFGSEFQISKTKYVGVLDQLRTIAKPRSPMPLLEEVLDWCVKVNEEVKHTGREIKLMLDIKSDNDPTVLMKKLWNECQKSRLKSGDSTEMIHYWKNKLILGLWDSKFYDPTLSKNFTIVNITFDILKAQNFYREIKEKDKDATLHAISMINLILYKSQDKEEMLRWAKEENVKLWFWTVNENADLQNIISTCSLPTGNSLLEGIVTDDPIKVLDQNAMVKTPRWKYNLKWWLKSKIYSIFLFLSRGGYNLSLFVNCLKRIGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU43</accession>
    <name>A0A1E3NU43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Replication factor A protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71103</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020672.1">
        <property type="nucleotide sequence ID" value="XM_019163828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU43"/>
    <dbReference type="STRING" id="763406.A0A1E3NU43"/>
    <dbReference type="GeneID" id="30180515"/>
    <dbReference type="OrthoDB" id="188186at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013970">
        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114:SF1">
        <property type="entry name" value="REPLICATION PROTEIN A 14 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114">
        <property type="entry name" value="REPLICATION PROTEIN A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08661">
        <property type="entry name" value="Rep_fac-A_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11497" checksum="99C4D1D0FC509F7B" modified="2017-01-18" version="1">MESLRVDATLLAQSSNKTVRIIGQLKSLDTGNSSASLDSNGLISVSSSSNTDELVVDNWYEVIGVVQKDLSVNVLQWFDFGKDFNGNAAKKLVEVVHRVPELYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND81</accession>
    <name>A0A1E3ND81_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_37632</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015193.1">
        <property type="nucleotide sequence ID" value="XM_019163096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND81"/>
    <dbReference type="STRING" id="763406.A0A1E3ND81"/>
    <dbReference type="GeneID" id="30179783"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF23">
        <property type="entry name" value="FERRIC-CHELATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="319"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ44080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="589" mass="66531" checksum="E8586DB93250E519" modified="2017-01-18" version="1">MDPLQKRDVFHDGVWCIADMGTTAYVAAKKQSQAAVAWADQALYGHNTVYFCAVVVAVFIAKRLVYFLVDNAARTTKTTAGRPAETCYCKLAALNRWVCYRRLPTMLCSLFQLPSSLGNFLLIAGGSLYVLCYTFIPKFWYRECRGFGSPPLAVRAGMQATALVPFLFILSGKTNLISQLTDISYEKLNVYHRWVSVICCLLGWTHTIPFYVQAVREGGRARLAWFQSHEDLFRNGIPPLVFLTILTVFSHSYIRAAWYELWLQVHWICALGFYISLFYHCYPEMNSWKYMIATAVFWVAQLGWRAVNKALLRPNRGFLRPNKCRMRRFTSNGPDEHYFEVVIENSNDFSWVPGQHVFLRIPGLRFLESHPFSIISHFEPRQDTDIRLVVKACGWGGLTDHIYQKLPDSGFSDSTVFIDGPYGGCSRRVDAFHGVFLLASGTGVSAVLPFLEESCRQLKNPDALTSAVNFSWIVRSSDNIEWVLPELKKIPDDKDDHFTEASPVPATSPAPVAATLQLGLINITNSKPEVNQLVTNTGVSLLEKNIFIVSGSDSMKVQASNAVAALQRHVLRRQSPIREIYLHSESFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDS1</accession>
    <name>A0A1E3NDS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015398.1">
        <property type="nucleotide sequence ID" value="XM_019164797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDS1"/>
    <dbReference type="GeneID" id="30181484"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="111" mass="12854" checksum="15E07AFC62C6B7EC" modified="2017-01-18" version="1">MNALVADTNISFIDDTNREKVFAVFDRLCSAIVALVILSSGSTMRLQEYPRLTQCAPQSMFLFQGDTEEYCYFSTDVNKNNSLKRRFLFVNPEVSKYLVHYCCIVKRILNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDU9</accession>
    <name>A0A1E3NDU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_37109</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015420.1">
        <property type="nucleotide sequence ID" value="XM_019163060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDU9"/>
    <dbReference type="STRING" id="763406.A0A1E3NDU9"/>
    <dbReference type="GeneID" id="30179747"/>
    <dbReference type="OrthoDB" id="425925at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019459">
        <property type="entry name" value="GRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921">
        <property type="entry name" value="MYOSIN HEAVY CHAIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921:SF2">
        <property type="entry name" value="THYROID RECEPTOR-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10375">
        <property type="entry name" value="GRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="6">
        <location>
            <begin position="395"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="149"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="53"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="495" mass="56870" checksum="13DB92DD77CE7040" modified="2017-01-18" version="1">MAKKKSKNNGAKKQVNGEVETVKQESVERKEEIETSHHEEDRDNDDNSSSDPVAHKEDDSSREQIEQLQKELESLKLELSNKEDTLSKITSEKDEEINKLKTQIQNDQSKESARDPELNDIKQQLAMKTEESNKYKASYDSLLSRLSQMKSVFSKMKEAESKFEAVEAENGHLKSQLQSSTSKLSSLESNMSELKAEVLNLNSECDKLTAKNSELKQKLDSQDFELENESKQLEVENKKLKRELKEAKSDLEEYLILIQEEKMSKTNMNQELSDAKSKMDTIVRERKEWEKKCQQSSQEVTSLKETIVQLKKNSEDVVNSFESQLKSKSAQFNELVNEINELKESTADKDAKLETLKGLENDLKEKQLLIGKLRHETIILNEHLTKAMKLIKRESGKETVDRELVSNLFISFLQIPRGDTKKFEVLRLIANFLNWDDEKKRHAGLISSANYRSNSNSVVEVPHPGRHASQSHGDSFVSLWTEFLEKESTPREDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE07</accession>
    <name>A0A1E3NE07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74611</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015478.1">
        <property type="nucleotide sequence ID" value="XM_019164819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE07"/>
    <dbReference type="GeneID" id="30181506"/>
    <dbReference type="OrthoDB" id="3994232at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="579" mass="66349" checksum="3E7CED570AF8F542" modified="2017-01-18" version="1">MAALKKRTGHKYSHSGCTNCKKRSIKCDENFPECLACQKKNLKCEYKQKIFVYQPFKTSSKQNKKFKKIEIDHLSKEPTSTYVPEAVGSFDVIDKFIDEFKENFDQGRQSNISGNKEEQILECKKLDELSASSALQVASYVRYWKPYTEERYQELFEKLDATGAKSSNVHYHENDPEVQEFILHAFVQSKATYNYILAPAHELSLVTNWVLHFGTKYPIMGYVLNFITCNLLDIKCSDDRWSCILKRNMATALINLSARVGQCDSFIEMVCYLFSIMFLFSEQTASRQNVWRLHLRGAFAILEQCSDLFNKVSQNKDPFDSDLKLALQMFSFSKNWFVAAEIIACLSAPNGGVIKDIYSSKFYLGYNTQELDDGVLLGGFNLMKGYSQLLSPVVMEIITFMIAFKKSEGISLSGTRGLLENLPYSEERRILGQKLLARVKEVQTEELNLSTMQDHVMRAYIRSCNKCFCYALQIYISSIFLDDFIYGDNIQHCVQIIEEQLVTSQDIRSYGLSIHWPLFVASLCALSANQRLTFIKALKNISSNGTFVARNTVERVERFWKVIDSGGLIEEEDYDCTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE88</accession>
    <name>A0A1E3NE88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ERAD-associated E3 ubiquitin-protein ligase component HRD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015542.1">
        <property type="nucleotide sequence ID" value="XM_019161955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE88"/>
    <dbReference type="STRING" id="763406.A0A1E3NE88"/>
    <dbReference type="GeneID" id="30178642"/>
    <dbReference type="OrthoDB" id="27934at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF160">
        <property type="entry name" value="ERAD-ASSOCIATED E3 UBIQUITIN-PROTEIN LIGASE COMPONENT HRD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133325" description="ERAD-associated E3 ubiquitin-protein ligase component HRD3" evidence="2">
        <location>
            <begin position="18"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="862" mass="98762" checksum="6835A0018E90F156" modified="2017-01-18" version="1">MVYIVNILTIFVTLVCADTSKESPIIGSARDALKLLKDQSGTDHKYTYEVPKGTVNNDTFFHEYFEYGREVGDESLNGNLTQNQNGFLIEISDESSVFFNIDDIEQYGATNFDIPYGPLRQIETDCFAEVESNWTNLQLKAFAVLEKLAIEEDSEALRILGDLCTFGFFNTKVDLDRAKAYYGKILEIESDTKTEAHAHFMLGVFYSTGLFGKTEKNQPKGLIHYRFAADLNNSQAQMALAHKYMFGVNVAQDYETAIYYFSLVYQKAEEYLEPFRTEITKTTTDNQNITERLLIFEPILDKFSVRWSDVDGGLYDSSSEAVNTVRYFETFDSYDKIKTYGSGNFNHEEFPDGDDETLDAYSMLYFAAQKNYNGDYLHARNFDLSFKYAKTCVHNGFLEPEIDELLKGKDQNNNIAYSYESPNYSFNFKLGIDQEVSPLSIFVGRCSQYLGHLYLRGQGTEVDYQKAKYYLEVGRVLSGSDTFINDIALMNQYGLGVPKDIIGVSRMYSEKQRMAPSTKYYKAVSLIEYWNENMKGDEVNIITNDIYLLLTSSATYNKLARRKLIELYEGGRMNKPLDIIVGYYNWYLKIFESMYFDFKIPFFAFLNAKADDTTSNNMWTALVGMAIESELGYEAAQSSLGSILYPRVGPYQSKSARNLPTVYSKIYTPKRFHEAISYFELSALHDNRDSINMLGDMYYEGLNSVPGADDPMWRNNWWTYVLPVTEISEKPDSFLFSAIELTFNRLQNLTRFFKMKFNSFFFPHQATPFERPVAIIPKALDRAVSYYQVAASKGSQIGSYSVGWAYEYGMGVVQDLHLAKRYYDSVMTISDAGYIPVKLAVFRVKMKTILWNVLGYDGNGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEE9</accession>
    <name>A0A1E3NEE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015640.1">
        <property type="nucleotide sequence ID" value="XM_019161991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NEE9"/>
    <dbReference type="GeneID" id="30178678"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF39">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE YLR345W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="3">
        <location>
            <begin position="70"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="499" mass="57493" checksum="3254906F91D6190D" modified="2017-01-18" version="1">MSTENEVAEIEEAKIPGNYSARKIQRWNPSSIYATNIIHNNFRNNVKSNDAIHKNLPVNHVSPAQMYSTDSGRMFHAGSICIIMVGLPGRGKTNLSISLCRYLRWLGVRTELFHLGDYRRKNTDLNKETDLNFDKLNSNSYFSLQPENSNIKNLKSKIKSELIEDIIGFFETSNGQIAIYDAINGLSSERKRLLDFFKSKNIKCLFIESIIDDVELLNENIRDAVNSPDYKSWDSEAALKNFTNRIKANEAYYEHLDSKVANLPFIQSYNFGEKILINDLKHDFLTTKIIYYLLNAKIKSHSIYFARCSLNKLKFKDDPPITENGKVYINKIYNTLASSFTDQKIPKDLVVWTSTRLRTIQSSQIFKNAGCTVRHRPELTQLNPGAAEGLTDQELDEKFHESYVKHLSDPYHHRYPRAESYHDLALKIEPLILEMEKLSHDVLIIADETIIRIFYGYLMASNANDIPLIEFPQNEIIKITYHTYNNEIENLVVDGIAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEJ3</accession>
    <name>A0A1E3NEJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome biogenesis protein NSA2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17991</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the pre-66S ribosomal particle. Interacts with NOP7 and RRP1. Interacts with RSA4 (via WD repeats).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eS8 family. Ribosome biogenesis protein NSA2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015680.1">
        <property type="nucleotide sequence ID" value="XM_019161840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEJ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEJ3"/>
    <dbReference type="GeneID" id="30178527"/>
    <dbReference type="OrthoDB" id="1847590at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11381">
        <property type="entry name" value="NSA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000001">
        <property type="entry name" value="NSA2, ribosome biogenesis homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039411">
        <property type="entry name" value="NSA2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12642">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NSA2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="261" mass="29714" checksum="49B68C5D77D81ADC" modified="2017-01-18" version="1">MPQNDYIEQHIKKHGKRLDHDERKRKREARESHRIAKDAQSLKGWKGKQFAKKRYAEKVAMKKKIKAHEESKLKGPSKPKEDDGEALPTYLLDRQQQNTAKAISSSIKQKRMEKADKYSVPLPKVRGISEEEMFKVIRTGKSKSKSWKRMITKHTFVGEGFTRRPVKMERIIRPSALRQKKANVTHPELGVTVLLPILSVKKNPQSPMYTQMGVLTKGTIIEVNVSELGLVTAGGKVVWGKYAQITNEPDRDGVVNAVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEK2</accession>
    <name>A0A1E3NEK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_17977</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015662.1">
        <property type="nucleotide sequence ID" value="XM_019161834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEK2"/>
    <dbReference type="GeneID" id="30178521"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="662"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="701"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="741"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="767"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="820"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="943"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="995"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="12">
        <location>
            <begin position="279"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1032" mass="117919" checksum="21F6AB8DEF5A1827" modified="2017-01-18" version="1">MDQDVSPEAENSHGIEKKSLQHRYFRKLARKQNDHMNNGVEGLGSGGTPETTEDTLGYFHIPSRSYSPERLTARSRSHSISPIRHPSVLTFGDDALSRSPSPTRRLLTSTHTVNENPFVTSDQPKYPYLYPTELRPVRKNSDNPFSDISSSSSFHASSNQKVGSDSTTNSVFDTYAFDEDGATDDLDNEFTPFPTLENSYYPINMPYDVYNPFEAEYTSDDGTNSVEENSNASSVSLERRDTAFKNAKAVKDTVFYYDKKDSQHQPEMAADNNGTNSATKQVRMVDGNLVLDCPVSEILLSKYKVPLTEKDREFLFMRFQACTAEPEYYQEEKFSLRQNFYTKPRETELMIVVTMYNEDEFLLARTLKGVFKNIRYLYNLKHSSTWGTNAWQKIVVCIVSDGRQKINPRSKALLAALGVYQEGFAKNRINDDDVVSHIYEYTTMVGISKIYKDHITLTTDNQIPVQMIFCLKEQNQRKINSHKWAFKAFAPLLNPKVIILLDVGTAPESKSLYKLWKSFKDDPQVAGSCGEIKASLGTGWRLLLNPLVAAQNFEYKTSNILDKPMESVFGFVTVLPGAFSAYRYEALIGEPLEKYLKGENLKKNNDDGIFNANMYLAEDRILCFELIAKKNSNWILKYINTAGAYTDVPEQLDDFISQRRRWLNGSFFAAVYSVCNFYKIWRTGHSVGRKLALQIQFFYQLLNLLVSWFSLGSYFLVFRILSCYLAESSIGFPPGNVLSVFFLWLYMASLTTTFVLSFGNKPQGTKIFYLIIVIFFAVLMCYMIFAAILLSVKSIQQILDDSKSGFSWKFLFVETQFRNLMIAILSTYVLYFIGFLMFLQPVHMLTSFVQYLLLSPAYVNILNIYAFCNIHDISWGTKGQDKIIDLGIAKKGISGNQDELEIVIPTTKYQIDDGYSKMVKTLMTPDPSSSDDNSIDPEEKNKFYFAFFRSMTVLIWMFSNFILVAVVTETGGLSQFDKNDNKKESNDILPSKQTFIFLSVILYIVAFMAFFRFVGCTIYLMQRGFNKLRVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NES6</accession>
    <name>A0A1E3NES6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_74358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015760.1">
        <property type="nucleotide sequence ID" value="XM_019164747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NES6"/>
    <dbReference type="STRING" id="763406.A0A1E3NES6"/>
    <dbReference type="GeneID" id="30181434"/>
    <dbReference type="OrthoDB" id="10260285at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="396" mass="45366" checksum="C50B9BBDC97FD536" modified="2017-01-18" version="1">MSGADRSSHGQRPHGHHSHKSNIQYVAKVRYSNNLPPPACPAKILKDDENESKDDNLNTLVTSFFRKENFKNLIRLNDDLGMPLNLINLPHKDTVNGLKHNGMNIPLHPSDQLILGDPSKTIKTKSDSVSFLRRTQYISSDIQNSQKANNMSKMNVKKDDWLDPKTQLKTIEDMFEAPATGLSSIRHPTKKHLKAKKVWNFLPDTSMLDQTYYDVKFMSSASISKNKDKSKDSIKSMNDPRLLTSLLREIELNKTTKLVSFYLTDNADAEIVKQKIDDETENAPIDDNELDEILKDPSNSSVYKKQRDYDGTTKPIEPLRQLVITFDEKSNNAYYVPVSGKLELKKCRIDPYLVPKIKEMTYDQIHMFIREPTNSEIENRDISRSAYDPMEFGTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NET9</accession>
    <name>A0A1E3NET9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein RMD9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015778.1">
        <property type="nucleotide sequence ID" value="XM_019161867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NET9"/>
    <dbReference type="STRING" id="763406.A0A1E3NET9"/>
    <dbReference type="GeneID" id="30178554"/>
    <dbReference type="OrthoDB" id="4081443at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="712" mass="80819" checksum="F92FA9C666E659DB" modified="2017-01-18" version="1">MAIHPLTHTTAAMIRNVPHISSQTSKISLQSCLTFNSVFQQPGSSCAAATVGTSSPVSLNSPVDHQHQPVAKSVAPRRKSSGGRAGNSGNGSHHQNSGNGNSNGSSHQQRRNFHISSQHYSTSGSTRTAASASSAAGKGASAALPDFQKLTKGVPKHTLPYFKQLMAFDECLAHNSFFDPKTGDVPGGTAQFWDSVEKIMPLYDDLLITGELNQRRIDELISLLRNGLRMHRFELSKLKKNIDRDLNSPLQQIQAYLKNSIIKISNDIVHESDESNSNKLYSAAAVNNVRISSRGLTNLFKAFTDMGLIDEAAVIWNKGKKDPKLYDLFTSESVLGSILTLLVENKEFNFQEIWSIYETIKNSKTENDIIHSELQVAMIRACLLKNKTEKALEIFKEITSDVLKFYSRKNSSPPVAVKNYMTMAHLSFIGYCQDFETANIFFNDSFENQMPYLTPIQINYVKKFMSNTWKNTKDFDKVSQIWISTWQYFESNNKSTTAISSSLNDAYLDIFFEKYSSFNNDAALELKKLLKNYSEIKKMDEPFFNCLITKSKRWENVNVFQSIVKVADLYNFPKTNVYHRCVLKASGSVELPIEETFALFKRLISSNIQLGSNHLTNADWFAFRDATIHSKFLNGEKIDLYFKLFKVCCKYFLNLQNFKLYMNLDVGLDQRYRKVFDEFQRINVQDVDLNELQWFNKCNAIAKYWGNRGVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEY5</accession>
    <name>A0A1E3NEY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="9">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_36723</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015803.1">
        <property type="nucleotide sequence ID" value="XM_019163031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEY5"/>
    <dbReference type="STRING" id="763406.A0A1E3NEY5"/>
    <dbReference type="GeneID" id="30179718"/>
    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20912">
        <property type="entry name" value="RPC3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="12">
        <location>
            <begin position="27"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="11">
        <location>
            <begin position="157"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="13">
        <location>
            <begin position="493"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="659" mass="73978" checksum="6C743CE7334ED1A7" modified="2017-01-18" version="1">MTASTNGAKLPSELPNTAKTQSPSCFLHSVILRAYVGEQAAYIYSCLHAQRKMTLSQLSQKSGIKGSNLRKVLVTLIQLGCVVYMGGNGKNNTYYCYNEEGCWKLTYIDEIVRHVREKFGQVHASVIQNVILRGHLTIGAYVSDLELKSEVDKIEHAFVELVEDKWLVHVKDMDFKPLSETFATCVRAATREFASNSGVQNHIKDVSTDAKDQTGLAALFNDDDDGEDDDEGDNASKGGKLLRRLNGNVPLTASFDRFLKYERDLQLVSMCRHRIGSITSKIYNVVLKRVEKSSREVRCIEGIVDRLVADASVGAGSAGTSTTGYDPATGRDLIKRLELKDQQKGMNFGAADILKELSLSNKYGITKEDLVGTIYNEDEGGDDMVSSKKRKLEEEEKTDGAYKKTKLSKLAAHIKTFDDDEDEDEDGNAANEVDNGFDLANNSENGEILTLILQHLKLLTIDKKLSFLIETTPGQFYVPFTMLQGQLPDYHMKQIVRTIFGNSSLRILNCIEEKRLIEEKNIAKSVLMREGDVRKMISVLIKFGLVEIQEIPRTADRSAMRGIFAFKVNKNYQRGKSMLGKCFMFNMGEVLEQMEQIKMENKILLDKISREDVRGHEVELLLESELAQLKGVVESEKSGLAKFLRLRAMGELFWFTDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF49</accession>
    <name>A0A1E3NF49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015880.1">
        <property type="nucleotide sequence ID" value="XM_019160058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF49"/>
    <dbReference type="GeneID" id="30176745"/>
    <dbReference type="OrthoDB" id="5382952at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="784"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="826"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="894"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1079" mass="120479" checksum="90C0F7EEA3A8C2FF" modified="2017-01-18" version="1">MDANSPNNAATGQGSDPQRPQPRMVGGGEAEKGVVRGVGRAGSSNSSSSSSLAKEGEVRKGGGGGGGLLKHKKSRGGFLLHHHRNKDEEEPEQGTPTGSPGSSSFFHIHHHHSSGAGAGADSHSQPSRHHQSLANILPGSRAVRSANMDSTRNTSSSSSSPPVPHPRIEHNTNYGPHETIMMNRSASTPFPSNPPAHLHFPHHIPADVAAPAAASINSANTAASSMKSSGKKIFSFTKKATRLAGDLAFGRFEDTMDYPTYAIPNSPSHSSQSSFVQPPSRSSSHSNSHPDSPSQSLHSQSHSQLQTQTQPHSQSQSPSHQSHLPQFHPQVQRQKYQKQQLTIEYHTKMKNILSLTMNHSMSFYIDFLRFFLKYMDDSELDDPFLYSVTRNSFWNTWYIKYLRSINETLDTRASLLAEKDQNQQKQQGNQGNEQISVSDSLWSDSHDRVSASDPKLNSGSNLASTSTFDSQTSSNSPDDIKSKIAEIPSIDEIDAEIFQDRKMVLLLLWHNQIINRLSNHASVIHKLLSSHAKNSEICIITSNRLKPYQSTPGNASSKLSKNHISPAHLELIDGWQLRHDFPNYKVSLVNISDRFQVLLDLKNSAYLPDDLQQQYPHFNDISFLTEDFINSNVKPKEKAFLSDYSSFTAPSIHKLPVRDESQILVYAPDFTRLIFNENDVNMGLTEFERVLKPGGSIFLILYDVLSFKNTYTVNTEHEVRLGEYIQIFINNEISKLSKLPNLSQIIIKILKGKSFKKIKYVKLGIPVISFNEQQHDYDEISEKISQEQMHQAPKKASAEPDILIPRPKAGDEKDADVGTDYDYSEESNTTLIQPVSKNRNATYVNVNSQNQKTDSAASGNTRHAATSPFENSKSNQQRQQRENRHKHIHEAKSASRNGSPYAPNPTNTFDNDYENDVHYEHRHEKENLGDISDQPIASLYCMLSSYIDFLRLSQIVDLHSWMSDPINGHVRNSGSFSGVAGLTETTVHTLRLWLDWKLHGFEGEMVRKIVCERIKEGNIDREGHDVELGVKVKIHDIGNLEKRENPLIYFDDISQDVFNDGILAGLDSIMLVTAEKGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF92</accession>
    <name>A0A1E3NF92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Interferon-related developmental regulator N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015913.1">
        <property type="nucleotide sequence ID" value="XM_019164784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF92"/>
    <dbReference type="STRING" id="763406.A0A1E3NF92"/>
    <dbReference type="GeneID" id="30181471"/>
    <dbReference type="OrthoDB" id="3995788at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039777">
        <property type="entry name" value="IFRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007701">
        <property type="entry name" value="Interferon-rel_develop_reg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354:SF1">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05004">
        <property type="entry name" value="IFRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon-related developmental regulator N-terminal" evidence="2">
        <location>
            <begin position="270"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="549" mass="60753" checksum="B42372F26831F535" modified="2017-01-18" version="1">MSKLHKSIHKGTPFSGNASDISDEFHSTLSAKLSSLSIQGNAHSASSSTPGSAESRAGSLRSPLSRQESRQNSMLSLLVDPEADESISHDQSDGVSTGSGADDNQNNSRNGSTRGSASRTRMKIYTVPDLIHVLGMSARESTRSGIGKNEREILFAHLYRMVISSNSEVYMGEAGANDEDFINLFNLKNSIDNTGSDDEGNIGFEWDAWIRCVDAYGCIGVDEVASDVVELVFPLLLKTICQLESAKNGDDIDLTTKRRIDLCIWSFMSLVLFIFYDSGNHGMLEHAKLFLRFIQKDTLEDDGIISSCLYMVGLALGLAWESGRGVKELIEDEVLETIRMILCSKKRKGSKLTAAVLAGLCFELLQKDIDEETEAADSDEEDTSDFSNEEFTEIAKELDLLANEGGKKSGKKGKVAKNVFREVLNTINRTPDNEELDAIAVSKSKNIDVRTWLTYIRVQVLRFVLGSELSTWLAKSRDMRNMLKKKRKDGKFSSSGGDEYDHNDDDEVVDTTIGGSAGSRDRKANKDLEKERTKQLNKERRAKEEELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFD1</accession>
    <name>A0A1E3NFD1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_78624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015960.1">
        <property type="nucleotide sequence ID" value="XM_019164963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFD1"/>
    <dbReference type="GeneID" id="30181650"/>
    <dbReference type="OrthoDB" id="10431989at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="261" mass="30195" checksum="6CC92F7F1BAF8351" modified="2017-01-18" version="1">MTLHVLCLQRLRRNQHWHEPTTGLTTYFELNDFRSWLKKEYFPDDSIVYAVEIKLIMYYLFFHFVSKEHLMSLDVASTGTMVVVTAMSSHAVGIFDRYLTFWNTAYFYLLNVGKYQNAVEMNKEIVDKIKVLRDSVGDATDKQAYIIYARLEDKARLLAEIMVLMINDHAFFSFPIKAPVLDETGTETLPVLCQDIQVILAVHRGLAHDTNGKRAPREFPKAFEGERGYQPLKNFSAVYAIILEKRDNYTDTMARILTSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFK1</accession>
    <name>A0A1E3NFK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Pre-mRNA-splicing factor PRP46</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Pre-mRNA-processing protein 46</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_35718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing and required for cell cycle progression at G2/M.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the WD repeat PRL1/PRL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016017.1">
        <property type="nucleotide sequence ID" value="XM_019162962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFK1"/>
    <dbReference type="STRING" id="763406.A0A1E3NFK1"/>
    <dbReference type="GeneID" id="30179649"/>
    <dbReference type="OrthoDB" id="10256122at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="1 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="360" mass="40330" checksum="750A4D830D4A5C3E" modified="2017-01-18" version="1">MTLYNDNSYVNLKNDNNLVIQPNEDAFANSLIVPNRDTGHITQKKNHSPWKLLKLLLGHTGQVTSVTMDPFNSFFVTGSADRTMKIWDLASGNLQLTLSGHIMAVRDMVISPRHPYLFSCSEDKTVKCWDLEKNVVIRDYHGHLSAVYTIDIHPELDVIVTGGRDSSVRVWDIRTRTPIHVLTGHRSSVNKVKCQSNDPQVISCSMDSTVRTWDLAAGKCMKTLTFHSKSVRAFCLDEGNEELITSSSDGIKKFKLPECQYQQDLQFWKNHNVVEEGNLIVNTMCNSTDGVVFAGCDGGKFAFWDWQSGEMFQNDVQVPVPGSLDSEGGILCSTFDHSGERLITGNIDKSVRILNKVIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFK5</accession>
    <name>A0A1E3NFK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17439</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016038.1">
        <property type="nucleotide sequence ID" value="XM_019161603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFK5"/>
    <dbReference type="STRING" id="763406.A0A1E3NFK5"/>
    <dbReference type="GeneID" id="30178290"/>
    <dbReference type="OrthoDB" id="239812at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000001">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9 11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="288" mass="31214" checksum="45A43D31A3E931AD" modified="2017-01-18" version="1">MYRLGAQQIARASCAQARNYATLREIETRLKSIKNIEKITKTMKIVASTRLGKAEKAMTSSRVYHESDIDFFKTAETAAPEGSEKTLIIAITSDKGLCGSIHSQMGKKVKAAVSEYPSADIVSIGDKTKNQVLRSNSDKLVLSFNGVGKETPSFNEASLIVDEISKLGDYDKIIVLYNKFVSSVSFEPSAASIFSKESIDASTKLSVFDIEDDDTTGVLAKVALTNAVFAGMAEGYASEISARRNAMDNASKNAGDMINKYSILYNRTRQAVITNELVDIITGASSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFL3</accession>
    <name>A0A1E3NFL3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17436</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016033.1">
        <property type="nucleotide sequence ID" value="XM_019161602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFL3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFL3"/>
    <dbReference type="GeneID" id="30178289"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133351" evidence="6">
        <location>
            <begin position="34"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="392" mass="45507" checksum="A99F920CBE06A0EA" modified="2017-01-18" version="1">MNTVFPSRAVGTGLAHLVLVFQTLFAVAVTATALGGPSGELTNSTLYTLCRNSDLYDLLDTIVNYEARFNHRYHYDWVFLNDNPFDDNFKLLVSNAVSGTARFGLISEEMWEIPESVDRHVMEANIARVMADPEGPYPYADSLSYRKMCRFESGFFQWHPLLLEYDYFWRVEPGVKLHCDIPYDIFRHMVDNNFRYGFTISMLEYSKTIPTLFNSLWEVLDRSCQLELLNSDENYSNYILNVDSNSYSLCHFWTNFEIGDLNTFRSKEYSYLFNELDKYNGFFYERWGDAPVRTLILSLILKYPQITRFKDLGYQHDPYLQCPQDLTIRAANRCSCDTKLDITNKWFSCSWYNKRRDGVRRLIPKKPSLQLAFDFAPALAPSSSSSSSSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFU3</accession>
    <name>A0A1E3NFU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exosome complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73794</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for exosome-dependent processing of pre-rRNA and small nucleolar RNA (snRNA) precursors. Involved in processing of 35S pre-rRNA at the A0, A1 and A2 sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the C1D family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016107.1">
        <property type="nucleotide sequence ID" value="XM_019164589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFU3"/>
    <dbReference type="STRING" id="763406.A0A1E3NFU3"/>
    <dbReference type="GeneID" id="30181276"/>
    <dbReference type="OrthoDB" id="1421013at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011082">
        <property type="entry name" value="Exosome-assoc_fac/DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341:SF3">
        <property type="entry name" value="NUCLEAR NUCLEIC ACID-BINDING PROTEIN C1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341">
        <property type="entry name" value="SUN-COR STEROID HORMONE RECEPTOR CO-REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="215" mass="24703" checksum="B6CAA814FA618AD3" modified="2017-01-18" version="1">MEETDAVKKLLITLGDLTGTISSKLKENVLEKTLSESLDEITRHKESNKANDEALNELEQAKILNMYAYLLVSLSFTNAKLDGVKFNNEAPIMQEIKRVKQYMDRVKKAEESLYESAEKARKREAESETFINKHLGEPAVSKVHFQDREETNKVQTESKNKHQYFKDHDNLKQALQEEKRNDSTKQSKSKSKNKNKGRQIGNVDTKNGKVSKSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFW2</accession>
    <name>A0A1E3NFW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016127.1">
        <property type="nucleotide sequence ID" value="XM_019164596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFW2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFW2"/>
    <dbReference type="GeneID" id="30181283"/>
    <dbReference type="OrthoDB" id="276296at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052848">
        <property type="entry name" value="CHCH_domain-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031731">
        <property type="entry name" value="CX9C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16860">
        <property type="entry name" value="CX9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMS import disulfide relay-system CHCH-CHCH-like Cx9C" evidence="1">
        <location>
            <begin position="12"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="114" mass="13174" checksum="767E5DCF487D9150" modified="2017-01-18" version="1">MVSRVLDEYIIQEVARTCPEPFLGFHRCMEDPAIKDKSECATFQRDLQHCVKTQVTVYHHIESKCSDIIQKYQTCLMKDTEGNTSSKCYNELKSLRECAMGVIQDEQQKHQAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG19</accession>
    <name>A0A1E3NG19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trichothecene 3-O-acetyltransferase-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_59893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016182.1">
        <property type="nucleotide sequence ID" value="XM_019163398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG19"/>
    <dbReference type="STRING" id="763406.A0A1E3NG19"/>
    <dbReference type="GeneID" id="30180085"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054710">
        <property type="entry name" value="Tri101-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF64">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22664">
        <property type="entry name" value="TRI-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Trichothecene 3-O-acetyltransferase-like N-terminal" evidence="2">
        <location>
            <begin position="38"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="466" mass="51207" checksum="77FC4EB5EEEE1910" modified="2017-01-18" version="1">MSSREANSTHTAPEGHSLLTPSMFEAINILGQRSQLRIYTQITLGYETPGDYHVDDAVSVFKAGLARLAACVPWLAGSIEDENGVTGISSTPGETISLAVEDMRQEESTPCFAELKRMGFPARFFSEKHFCSRMTIPGTHGETLGGDEPVFLVQLNIVDGGIFLSFIASHAVLDMVGQASVMRLLCELCDPSKKPSHEALLNTQLKRSTIPIIPDTPSMAAAEELFIQKPISVAPEDLSGAWRYFDFTKEKLERLKTACAKEITETPFISTDDCLCALICKTVTRARSNRVDPSRHMKFARAVDARSLFEQDPHQIGVCLNMTFNRWPIREVTDQPLGVLASQLRGKLSDRVYLRETVMVFGSMLSRAGEAGTDGPLLSMAGTVDPSVDVQISSWSKLDCYENDFNLGLGNPTVVLRPTFVPVPGLVYFLPRRSDGGVSVTLCLRDDDMTQFVSDPLLLLYGTPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG38</accession>
    <name>A0A1E3NG38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_37403</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015641.1">
        <property type="nucleotide sequence ID" value="XM_019163083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG38"/>
    <dbReference type="STRING" id="763406.A0A1E3NG38"/>
    <dbReference type="GeneID" id="30179770"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="608"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="822" mass="92090" checksum="714DE83092E320F2" modified="2017-01-18" version="1">MANTNYTSINDRELSYIEVPEYNDKELVGGDDYVKNVFRSPVKRSLNYVKGLFPFMEWFPHYPSHPDWILSDFITGVTVAIVLVPQSMSYAKLAELSPEYGLYSAFIGVFFYFIFATSREICIGPVAVLSTEVGKIVTRVQAKYGDEFTANEIATTLALISGGIVLGIGLLRLGFIVELISLPAILAFTTGSAFNIVTGQLAGLMGYKNKVKKEDTTYLTMIQFLKHLPDTKVDAAYGLVGLFILYLWQFVLNKLIERNKHNTRRKMAFIYLLNLRTAFVIIISTCISYGILRYHPKKEKPPYSIIGVIPSGLRHVGRFVPKPTLAAKLASDIPISTIVLVLEHISISKSFARINGYRVNPNQEFIAIGFTNMVGTFFNAYPVTGSFSRTALSAKCGVKTPFKSAFSGGCVLLAIYCFTGGFYYIPNATLCAIIIHCVTGLLASYDLTAKLYMFSPVDFVIFIVGVFITVFASIEDGIYWAICASCATVLWRMLLPNGAFLGRVKVATIRNPLLVKNARPQNSEQQTDLDSAIEEAKGDSNSIQQLKNDNADVTSFVKTTEVETTVSISSLTSYQYKWVPLPNNASNPSFVHTRYVNAQINVEDPPSGILVYRVSENFIYTNCSLQIDQIISQVRDTMRPYKSDRERLWCEYTWEEKNWDFNFKKADEADDGTLKPKLRILHLDFSQVVAVDSTSVQALLDLRKTINDYCGPDWEMHFSGIINPWVIRGLLNAGFGDNSPDKADDLVDEYLKEEFRSIPLWKKRVADVDDDSEFDEETGSQKVVAHGGDLEVGIAEDGKMYPIFSTNHPNFHVDIPSYTEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG52</accession>
    <name>A0A1E3NG52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pyruvate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_36220</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016224.1">
        <property type="nucleotide sequence ID" value="XM_019162993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG52"/>
    <dbReference type="STRING" id="763406.A0A1E3NG52"/>
    <dbReference type="GeneID" id="30179680"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="12">
        <location>
            <begin position="12"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="10">
        <location>
            <begin position="219"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="11">
        <location>
            <begin position="422"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="65445" checksum="700DA984DDFDB581" modified="2017-01-18" version="1">MKAGSLNKSEITISEYIFHRILQLGIKSVFGVPGDFNLRFLEHIYDVKELNWIGCCNELNAAYAADAYGKTSGKMGVLLTTYGVGELSALNGVAGAYTEFAPMLHLVGTSALKFKRDPRTLNLHHLAGHRHTWKKSDHYVYEKVAENFSVDSASLEDDPEEACEMVDRVILSVWRNSRPGYIFLPCDMAEMKVDVARLSIPIKLEYEFNTHADKANVYVEKILAMMYKSKNMSIIADEFIRKFRMEDEMHAMLKKLDGKVNLYSTMFSKGLVDEEDPRFVGTYFGKYESPVAKIIESSDLVLHLGKFDNEMNCGHFTFNLDQDKLVEFNPQYMQIGKEFDETVNMMQILPLLVEKLDGQKISPAVKYERPKKLYELDEAPREYSLTEVDLVKSLNKNIRPNDVLIVETCSFLFAVPDIKFRNAKVILQGYWASIGYALPATLGASLALSDFNLPGRVITIEGDGSAQMSLQELSSMLRYNIDATMLILNNSGYTIERAITGPYSSYNDINADWQWGDMLKCFGDLKQEKSETFKVQNPAELDKSLGSENYANGKFKLLELILPMFDVPEKLTNAVSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGF4</accession>
    <name>A0A1E3NGF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein SLA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_17656</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SLA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016279.1">
        <property type="nucleotide sequence ID" value="XM_019161682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGF4"/>
    <dbReference type="STRING" id="763406.A0A1E3NGF4"/>
    <dbReference type="GeneID" id="30178369"/>
    <dbReference type="OrthoDB" id="26539at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11773">
        <property type="entry name" value="SH3_Sla1p_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11774">
        <property type="entry name" value="SH3_Sla1p_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11775">
        <property type="entry name" value="SH3_Sla1p_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.700:FF:000001">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.700">
        <property type="entry name" value="SLA1 homology domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056996">
        <property type="entry name" value="PH_SLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007131">
        <property type="entry name" value="SHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035800">
        <property type="entry name" value="Sla1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035821">
        <property type="entry name" value="Sla1_SH3_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF19">
        <property type="entry name" value="ACTIN CYTOSKELETON-REGULATORY COMPLEX PROTEIN SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24081">
        <property type="entry name" value="PH_SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03983">
        <property type="entry name" value="SHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="4"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="72"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="427"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="161"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="487"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="676"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="795"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1222"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="531"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="687"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="831"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1231"/>
            <end position="1243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ45166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1273" mass="135997" checksum="9FEC7D7AF026D512" modified="2017-01-18" version="1">MSAPFVGICTALYDYQAQNDEELTINENDILYLLQKSDFDDWWLVKKRVLDANAEEPQGLVPKTYIADAAPVARATALYDYTKQTEEELSFSEGTVLNVYDTSDPNWALVDINRQQFGFVPGNYIQLDSGTAQQPSLQQPAAANPVSNVMGLTPFPEPVVSPANITPLSQSFPPPPQRAARPSSSNATPPAQSSTRSQFPSSRDDDYDDVDDADEARPPMPTRPNTTTNNNNSNSASSQRRSNTYNDYSEDDNDYGDGDSDDNGHAKESKTKSFFSSSSSKSRSRSGTETSKMSRKKSISNFLSSHKDDDDAQPVHGDFAWKIHEIDGKKKRSGVLEIGNGNVYLDVDNKLSESWPITNLTNYNAEKKHIFLDFKSPSYSFELHVSNKSSAQAIMSILGDMKGLQSQSALADIKRAQREGAEAGAVSTLKKGVILYDFDGAADDELSCYEGDTVLIVNDKKNKEWWMVENSRGKRGVVPSNYVKLVSNKSKSGSGNGSAGASSSSKDGGASGFLKNLLGPNSKSPKKKGSKDRDREERELREMAKLQHERGELQKQRRRMEQKEKELSYRDRDQREKREKIRRADERLRERRLRESSKSSSANSEKDSSKPNTHRVRTWIDRSGAFKVEAEFLGVQQGKIHLHKTNGVKIAVAASKLSIEDLEYVERLTGTSLDKYKPEISKPAEPTSASNNDSNTANTASTPSLPSRESSSAVARQRTSSVPLRADLVDYWFNFFVEANVDVNVCDRYARNFAQEQVDNTVLDGVTPALMRTLGVREGDILKIKKKIDQALGRATTPDPVLGKNPTDPLPNTPATSGMSDDDWATKPAARSTTEPVKPITSPNASKSLTPQITGSIKDLLDVKPMEPTRTGITPVKPINTIGLASSNSATATGGNATGPLTGSATGNLNLQKTGAGMAPAFTGAMPMMAMPTGFMPITMVPMMTGMATGMGPLRTGGLTTGLTGGLNSAFTGGLNPAFTGGMPMTTFGVNSLAQQPQQQKTGGAYIMPLTTGSAMPATSFGNYPSMNSQATGGMVRLQPTGVRLQKTGGGLSSMPAMQPTMKTGGGFVPQSSFGIQLSNAMTGGGMMNPAMTGGFPQTSFGNPAMTGGLPQSSFGNPMMNPAITGGLPQNSFGNPMMNPAFTGGLQQAQYGNGMMTGGLPPNSFGNGMMNPALTGAMGGNGLPQTSFGNNNGMMNPALTGGLPPTSFGNGMMNPAYTGGLPPTSFGGAPNNGSQPVLQSQPTGFGFGNSIVPASTGKQANLANATADNPFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGK2</accession>
    <name>A0A1E3NGK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74042</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016384.1">
        <property type="nucleotide sequence ID" value="XM_019164659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGK2"/>
    <dbReference type="GeneID" id="30181346"/>
    <dbReference type="OrthoDB" id="5322661at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014801">
        <property type="entry name" value="Mediator_Med5_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="957" mass="110455" checksum="4C5BC51F8A139D07" modified="2017-01-18" version="1">MEPTFSNLLKISLERQYPPTTFAKLFRDFEKKQANTNNGRIDTTPLYIDSGDNYLQRSYIFEILVGSDLENNFEEFINFFSVVSEKLKFNDQHSVLLFINQTAEKFPWKNYAQNVLVLNCLSAYSKYLVSNFVESEEFTRISNLFVKILINIFKVSNAFKLNENFVQTTTEFKLDENFVQSTTEFMEWLNAKSLHDAYKPLNFYLGEYVKSSVKFGFSSNDPSHLQGSELVDSLNFSKRKPAKLSEKDNIKLLKIKKIIWLSQKITIEFVNLNDQFIIDFKNLISLSNVSTMESVSSLSMELLSGIIQCIKISNSRNKAIFREYLVCKIPWFLKYVLKINQNKLEKVFETLSNDESEFFTSDTNLLFELQTNMIELELLRENVLEFEKGNKLLTDDQREQSYTIEELNQNYTYKFIECNPEFTSIEETGIVDFVKKVNKSIRLKHKFCDLFMESLDSFMLTGDTLRLRRLLISASLNFDVLDNLLLYSSPYKLLIPLLKFLENQVTQTPENAGMNTEERYLDQSNQPDLMMDLDIPGDDSSNAQDYFSDLSTILVFSQFIIARYALTLKECDLSAIPKTLSLLKNTGLIYDKDTKDGNPFQETKLDDEIVNKWISSMFDPSNVDGISDSLIKLSTPLDYSQLIPRIVNEAIICNNMGWLDDDTLMGGLEYMHQKFLVGWMAYVIEEICNLKWKNGGKEPKMDAILEKVLQQLLETNTSDSIDIQVIIKLVKETEKEKISLNFPQVKLDEQISAGKLTLDQSCRNIIKFVSPNEKDKQEHPEFELAQVWSLLLSQNLAVDYLYEELKSLIQDSQISAELSYELVSILLVMFARWVVGDSIKTKWPVSLAQINDSDQSATELQMLSLIFNRKPATIAVKGYKKRIETKTENDENKVESGFFGFIQEPKSDESDNNMTNLVEEAEKGIDIYGENLLLIAYEHKGDRTTETFLRKVLDHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGV3</accession>
    <name>A0A1E3NGV3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17856</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016498.1">
        <property type="nucleotide sequence ID" value="XM_019161780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGV3"/>
    <dbReference type="GeneID" id="30178467"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="52" mass="5738" checksum="0CCFB4538B4921CB" modified="2017-01-18" version="1">MCLCAYVRACVPSLFCVFCSPHSLSIPPAFLPPPVRHGRPLSFYTCPVQKPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGX9</accession>
    <name>A0A1E3NGX9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17798</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016449.1">
        <property type="nucleotide sequence ID" value="XM_019161746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGX9"/>
    <dbReference type="STRING" id="763406.A0A1E3NGX9"/>
    <dbReference type="GeneID" id="30178433"/>
    <dbReference type="OrthoDB" id="339151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990861">
        <property type="term" value="C:Ubp3-Bre5 deubiquitination complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF20">
        <property type="entry name" value="AT27578P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NTF2" evidence="3">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="248"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="306"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="378"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="576" mass="61708" checksum="55375CFC23B508C5" modified="2017-01-18" version="1">MTLADYEKDPNHITNDFIKYYYSKLSSDCSKLYQLYAPDAVLKHMDHTSDPFKAISTNYHDFDNIKKYWKSLPSLSGAKLVILTVNTIHNSDNSLVTNVVGELLLREDYEIESVISPTRLFTQTFILQPHPSKDNYDIRSDILTFIPDSDYMNVELNGVSTSVSQDSHSADAFQKSVEEKPTESREQIQQEVVETPPTSKLNNGFNGAENGTSSDSKKYTKNTIPTSSSPSSPSSLATANNTTEQKKSTPTSQPITIPSSSSSEIPSSSTTATSVNTLTRSAETNKSNGVNPTATTSSEPGKKDLTTNSTKIPAQNGTQAAGGAENQTHPQQISTENKNPTPVSSNTPLSTTTIKSNKPSTEASKLHSASPTPPPSSAPSVKQKASSSSSSSSPAAPAQEAKAPAASQPASSASSTWASALKITNKPASPLLSQSQTVAQQSHSQPSPKSASTQLSSSAASSSTVTSSASTSGNANQLNRPQLYEIYVNFKNSAVPVDEIDLKSAFSSNKFKHFTIVTINKNNTAIVGFNSQEEQARALALGKIIQGETQFGIDKREKKTNGKKRSSTFKNANKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGY9</accession>
    <name>A0A1E3NGY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_17811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016459.1">
        <property type="nucleotide sequence ID" value="XM_019161757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGY9"/>
    <dbReference type="STRING" id="763406.A0A1E3NGY9"/>
    <dbReference type="GeneID" id="30178444"/>
    <dbReference type="OrthoDB" id="15688at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008111">
        <property type="entry name" value="RNA-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45894">
        <property type="entry name" value="RNA-BINDING PROTEIN 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="115" mass="13787" checksum="359543360376A881" modified="2017-01-18" version="1">MTNLHPETSEDHIYDVFEEYCRVVNLHLNLDKRTGYVKGYAFIEFRKLEDVKEIMELHKHQKFQILGRTIELDYAFVERPDWSDMARLKSRSVSHKNVRERLEGLDTRDFSPTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH25</accession>
    <name>A0A1E3NH25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inosine triphosphate pyrophosphatase</fullName>
            <shortName evidence="10">ITPase</shortName>
            <shortName evidence="10">Inosine triphosphatase</shortName>
            <ecNumber evidence="10">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">XTP/dITP diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HAM1</name>
        <name evidence="12" type="ORF">PICMEDRAFT_74214</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10 11">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="10"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016568.1">
        <property type="nucleotide sequence ID" value="XM_019164711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH25"/>
    <dbReference type="STRING" id="763406.A0A1E3NH25"/>
    <dbReference type="GeneID" id="30181398"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009204">
        <property type="term" value="P:deoxyribonucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000009">
        <property type="entry name" value="Inosine triphosphate pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03148">
        <property type="entry name" value="HAM1_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027502">
        <property type="entry name" value="ITPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="9"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="194" mass="20748" checksum="48E6F64CBFE4B28E" modified="2017-01-18" version="1">MSDTITFVTGNANKLREVTAILCNGQTEGATVGKYSITNQKIDLDEIQGSIEEITIHKAKQAAATVGGTVLVEDTCLGFNAYNNLPGPYIKWFVNSIGLGGLVKMLDGFEDKSANAITTFGFCDGPGKEVQLLQGVTTGTIVDSRGPTDFGWDSIFMPDGFDTTYAEMKGEAKNKISQRSKALAKVKAYLTGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH46</accession>
    <name>A0A1E3NH46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_73696</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016001.1">
        <property type="nucleotide sequence ID" value="XM_019164556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH46"/>
    <dbReference type="STRING" id="763406.A0A1E3NH46"/>
    <dbReference type="GeneID" id="30181243"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF243">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="125"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="196" mass="22258" checksum="31FAB49858E0E60D" modified="2017-01-18" version="1">MSNYEEEHNLNNRSVDGSLSSSRERNASEDRPDHERHPTLQQALDNFLNTPSYSTTNIVNFNRESLQASGLAIEPQMLEMLQSLVTSSNGVISFQDSAESKGVDDSFLDTLERVNVKKLKDDDTCPICTNDYKSDKHPLVVELPCNSQHRFDLECIGPWLKLNRTCPLCRTDVTVKKNKVELVDSEEEDEGWDMYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHC4</accession>
    <name>A0A1E3NHC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquinol-cytochrome c chaperone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_18134</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015856.1">
        <property type="nucleotide sequence ID" value="XM_019161891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHC4"/>
    <dbReference type="STRING" id="763406.A0A1E3NHC4"/>
    <dbReference type="GeneID" id="30178578"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061671">
        <property type="term" value="C:Cbp3p-Cbp6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021150">
        <property type="entry name" value="Ubiq_cyt_c_chap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007129">
        <property type="entry name" value="Ubiqinol_cyt_c_chaperone_CPB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX ASSEMBLY FACTOR 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03981">
        <property type="entry name" value="Ubiq_cyt_C_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquinol-cytochrome c chaperone" evidence="3">
        <location>
            <begin position="148"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="336" mass="38426" checksum="3F351509534C73E8" modified="2017-01-18" version="1">MLSTAARQAHILAKSRVPAANFGLAARRFASSQVQNEASKEDKATAVPSPEDLAKASHLPHISNVEMPNKDINLKNKLEKPDYEKEGYQVPEWKKAFGETFVSLFHIDMDNVRAGTIGGSKYYYMCKDQGLQFTDEELSETAVFWYDTLGLPRTFAMWFQITTLHVWLLFVRMRAMPFKMGKNYQQKLVDKLFKDIEMRLSEEMNVLSGQIRDSYMKDFHSQMLGMIFSLDEAFAQKSDDVLAAAVWRNVFNGDKNIDIVKLEAVVRYIRMQLYVLDSISDRAFGFGDFEFVGPNETVDLLTAEDKAQLLKMTKMKFEPEDGKTLPSQKSKLSLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHN1</accession>
    <name>A0A1E3NHN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_16932</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the BBP/SF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016750.1">
        <property type="nucleotide sequence ID" value="XM_019161399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHN1"/>
    <dbReference type="GeneID" id="30178086"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045131">
        <property type="term" value="F:pre-mRNA branch point binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02395">
        <property type="entry name" value="KH-I_BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="15">
        <location>
            <begin position="310"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="346"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="471"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="488"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="595" mass="66048" checksum="2E0DDC7C574052A3" modified="2017-01-18" version="1">MDSMANATFEEGVREKHKNGVNKSIEKSRGRTRERLFSSHHWSGPPTNAIYMEEAIATPTIIRNTLTPEQIEAYVILVRIEEICNIISSGNYVPKDERLRSPSPPPVYDASGRRKNTREVRYKRKYENERCFLVERALKLIPGFKTPDWYNPRPTTIVEKLYIKTKEYPQINFIGLLIGPRGNTLNKLQEDSGARIGIRGKGSVKEGKNTMNISPELNNLQDQLHCLITADSVEKVEKAKKLCQQVMDKAIFSPAGQNDLKRDQLRELAKLNGTFRDDTERACPVCGEMGHNRNTCPNKNRTNFVNSLVCSKCGNVGHLEKDCKIQSKADDHMDQEFADFMNELNEDAEGTGNDGGISSKDGDFKDRVPLHQPHMIPTKPSLQNNDNFTSNNNKRFSEHSHYDSYDGYGGGADGAGGANGYKRQHHGDAYSSQGISGYRQGYGHQYTQSYGGDRYGQDQQYQYRQSYGSRYGYGQQQDHQHHSHGKQYDTQPPSYSSSINPFDKQSRATHPRQQLPPPPPTSSSATIVSTTNYAPASTTGGALSVGLQAPPGLKNRSSVFSPPPPPPPPGGGSNSKTGTSLRKQVPPPPPPPPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI13</accession>
    <name>A0A1E3NI13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_59396</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016885.1">
        <property type="nucleotide sequence ID" value="XM_019163363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI13"/>
    <dbReference type="STRING" id="763406.A0A1E3NI13"/>
    <dbReference type="GeneID" id="30180050"/>
    <dbReference type="OrthoDB" id="14535at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20266">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045299">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016488">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="122" mass="14808" checksum="799B4851341863FC" modified="2017-01-18" version="1">MTFPTAFAETTRFSTTSADLRYRVLNLYRRYLRHSREFVNSYDLDIPAYQVKTKIRQEFERHRFVQDLATKNVLYMKGQMEFQELVNFWKQQCHVMQYFEDFEHYSTTSGNSFVQKFLKGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIH9</accession>
    <name>A0A1E3NIH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017055.1">
        <property type="nucleotide sequence ID" value="XM_019161501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIH9"/>
    <dbReference type="GeneID" id="30178188"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17316">
        <property type="entry name" value="Perilipin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="267" mass="30589" checksum="BB621E0784A4C070" modified="2017-01-18" version="1">MGIAYSSRLNSYTTSVDAYLDSQLNALDQAAPFVKTLRMRDIRNVILDDPIRYVAFRTANSLRDISDLTQRAVIKPSREGIHQLRDLRGEHLPFTGNQPLLRSQLNPIFRQVNQRLIEDINTYFPASSNSEEIPINYVTFDNQSNELSYTLRLINLAVLRARPVLQERFQQLTETPSEARKYVAAVYQESQEHRGEGRIVIIIATLETIRKILTDGYEAFSANRFFQFLLSEPTAEEKVSIAEETQEEENDDKADNKNAEEDTTKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIJ6</accession>
    <name>A0A1E3NIJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MICOS complex subunit MIC19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017088.1">
        <property type="nucleotide sequence ID" value="XM_019164481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIJ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIJ6"/>
    <dbReference type="GeneID" id="30181168"/>
    <dbReference type="OrthoDB" id="5544375at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012471">
        <property type="entry name" value="DUF1690"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07956">
        <property type="entry name" value="DUF1690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15689" checksum="4BBD9F9763D9F86D" modified="2017-01-18" version="1">MSESKAFPTLTPVQFSPNLLDSLDKSTDSTVTRQQRQSQAISAQVGKQLDELLSEKAEKLDKTIESKLLAPESDKQKLQGSPELNAKLDSIYNTLKQSAEAKVAKSPSVLAAEKAVSQCLLKNKGTPLNCWDEVQSFKKLAGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIP7</accession>
    <name>A0A1E3NIP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017117.1">
        <property type="nucleotide sequence ID" value="XM_019164492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIP7"/>
    <dbReference type="GeneID" id="30181179"/>
    <dbReference type="OrthoDB" id="10448101at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="447" mass="50862" checksum="A19DC05A26176C33" modified="2017-01-18" version="1">MTVFTTVVCPADLHDARFHNPTKIAPTIQYQIQPTSYFTLYHIQQYINDLTLLPNRIHANSVLSFSVSNSSFSSYRTSCLLKQHLNILWENCKIQRIDLDFTQFKFKREILQRVVALTNHIQKHGKYNVFLSVTVPLDKDLVIPEEFIQILRRFKQEFISFSMLNILITKNLKRKNVEWLEMAKMAYKNVASQLRKYDAANEIFIGSIEKYIGLSFDCDVTPNIHHYDVKTLAKLISPHSSSSYSSLNTHSMKTTVRQSVKHGEKKNMSELDFLMIWKWANKAGLGQVQLKSYLANSKNVKKVIEKNLSLLKPNAGTDSSLTLPQDMLYRLADEVGIRNITTVNYLEPAPFEQPTSRSASTTSALSSTQSSSPSTSSTETVNTLEPTQMTALAGRVAEPVTLLTVDNEYQHSLPDYETAMLHKVQEHERMMNSLAKLPSYAAETYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIR3</accession>
    <name>A0A1E3NIR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17212</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PIR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017069.1">
        <property type="nucleotide sequence ID" value="XM_019161512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIR3"/>
    <dbReference type="STRING" id="763406.A0A1E3NIR3"/>
    <dbReference type="GeneID" id="30178199"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133383" description="Cell wall mannoprotein PIR1-like C-terminal domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="9">
        <location>
            <begin position="297"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="379" mass="39093" checksum="CD455008156DE15F" modified="2017-01-18" version="1">MKFNTLLHLASAIALAKADGYVPGENYSTFTPTDGYLDGATTQFPQRFGIAINPITSSIALTSVTTSVDGTVVVTQIGDGQIQATTATPSPVVVTQISDGQIQASTYTPTTYEYPVVTQIGDGQIQATTLTTVTLSSASTSSAEADTQYRPLISTSSTSSTFSTFSTSSTSSTSSTSSASSASSTSSTSSTSSTSSTSSTSSTSSTSSSTSTFSSSSSSPLAPSSTSDDITTTSTVTPTSTVYSTVTSATLPTLDPTTVVVQKRFAEETAAVSIDKRDLPSTCSTTSSLSMTLVDSILYDSNGRIGAIVSNQQFQFDGPPPQAGSIYAAGWSIVNGKLALGKSTTFYQCLSGNFYNLYYNSIGEQCTAVELDVVVFNEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIY4</accession>
    <name>A0A1E3NIY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_17270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-25S rRNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM RBM34 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017139.1">
        <property type="nucleotide sequence ID" value="XM_019161528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIY4"/>
    <dbReference type="STRING" id="763406.A0A1E3NIY4"/>
    <dbReference type="GeneID" id="30178215"/>
    <dbReference type="OrthoDB" id="442677at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="281"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="416"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="456" mass="52324" checksum="E806AC75A35A56F3" modified="2017-01-18" version="1">MFGGLFAKPSVIDNEKLVSEKKRTIVQLRKKERKERKREEKREQRTNEESEEESELEAEDEDVEEKTQAAEKDNGDVRMDVDEQDEEDPGLEDKYMQKLLQDDEDEEEDEEDDDEDDKENSADEDAEKKQEEKAAGEGDSPAEGEASERAASAAKVLDLKEKEFEKAERTIFIGNVPSLIMSSKRDTKDFKQFINKFLETPENTSSIESIRFRSIHSTSNAPRKVAFIAKDVDLENVMNSYVVFKKKDDSLKALKLNGVLYKDHHLRVDHLTHPTKKDNKLSVFVGNLDFQEKEESLWKYFNDKLIAKEDKKDTTNVIDNVRIVRDPKTSFGKGFAIVQFSDTNYVTKALLLDGKKMGKRSLRITRCKKPMRNNEKMRSKFSKLNDQQKTVVGRAKQLGKIDRRTLGQVVIEGDRATKGQTVGGMGKKRHGGRKEKRAKKARITKRSSAFKEKSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIY8</accession>
    <name>A0A1E3NIY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16851</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016661.1">
        <property type="nucleotide sequence ID" value="XM_019161364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIY8"/>
    <dbReference type="STRING" id="763406.A0A1E3NIY8"/>
    <dbReference type="GeneID" id="30178051"/>
    <dbReference type="OrthoDB" id="10252509at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905754">
        <property type="term" value="C:ascospore-type prospore nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="6">
        <location>
            <begin position="47"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="7">
        <location>
            <begin position="926"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="673"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="686"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR015965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="983" mass="109386" checksum="B85D2B79786AC025" modified="2017-01-18" version="1">MAPVSEKEAAVATESAKTVATSSNKDQKKKNEEEELNEEDQQLKNELEMLVERLMSSQTELYKPALDSLKTFIRESTSSMTAVPKPLKFLRPFYPSLIQLDQSWKDKELLMQLYDVLSVLGMTYSEEDGEEYVRVCLKYRLKSHIDDTMSEWGHEYLRHLSLEIGDEYEAGLDAGEPKDKLVQLSSIIAPYFLKHNAEAEAVDLLLEIEEVEKLSKYVDKNTYKRVCSYMVACVPLLAPPDDLAFLNTAFTIYLENNRLTEALTLAIRLGDESLVKSVFESTNDVSVQKQLGFILGKQNYGFSVENEEVQQCINNNHMHRYFQYLVTELNLLAPKVPEDIYKSHLETSFYASSSKLESAKQNLAAAFVNGFLNAGYSDEKLISKEKWVYKTKDEGMLSTVASLGLVNLWNVENGLQSLDQFQYNENTKLRAGAVLGMGISTNGVHDDVESTYYLLEEFVHNRERDDELIVTYAITGLGIAYAGSENENVLNLLTPIVSDSNYTLEIQSLAALSLGHTFVGTCNGEITDTILQSLLDKDPTQLASKWIRFMCLGLGLLYMGKYDQVDDVLETIDAIEHPISKSLKVLIKICSYAGTGNVLQIQELLQMCTTKPTDSSEDDEDDEDDEDDNDFYEMEAAADAEVDAELDNDGGVMETDNQDNVEIADMPVDIENNAIEGGSDPTVKPSIEKGEAKIEDSKTETVSSEEASDAKKEDDLAEDDLYQGFAVLGLALICMGEEIGQEMSIRHFEHLMHYGKAIIKRAVPLAMGLVSASNPQMNVFETLSRYSHDQDMEVAYNAIFGMGLVGAGTNNARLAQLLRQLASYYINDQNGLFVSRISQGLIHLGKGTLTVSPFSIDKQILSKVSLAGLLTVCIALLDPKAFILDQNSNLLYYLTPAIRPRMLVTVDENLEPIKVNVRVGQAVDTVGAAGKPKTITGWVTHSTPVLLGFGERAELETDEYLAYASSMEGVVILKKNPDYVSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ26</accession>
    <name>A0A1E3NJ26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_74118</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016466.1">
        <property type="nucleotide sequence ID" value="XM_019164682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ26"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ26"/>
    <dbReference type="GeneID" id="30181369"/>
    <dbReference type="OrthoDB" id="4494341at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133395" description="FAD-binding FR-type domain-containing protein" evidence="14">
        <location>
            <begin position="19"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="404"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ45353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="706" mass="80691" checksum="915F7C933B528D5D" modified="2017-01-18" version="1">MKIVDVVLLSTLLSVASASHQWYHYNNDFYVYYGCLSALGKSATFCKKGNVGKCVCTNENALGSLLYCGYTSTKANNTGINKEISKGCVYYNVTDLTNSYYAEVYAKTLDTIVDVSNSTSFNKTEVVDYPVTSKKLKKLSHDFYISVRNRRGNIKTSHALGIAFVAASVFVMLASGVVNWSMRLSKGVSSSLDNALTRKTRKFFNLAIFRNHLHPTVLGFSPDVVETFWISIMFLYSILSCCIIGYKWYENDPSFTSYQTGTSRYWGDRSCILFSYQLPLLFLFPGRNNFFQWVTRWKYSRFVVYHKWLGRIVMMEVLIHSFAMASQTYGLHKNTRFHADWYRYGIVSTVCGCLASTVAIYAIRKHWYEIFLGAHVTLVVMFLWTGWKHTRSQLYEQFYWTCCAIWVFDRFIRLCRIALNGIHTATIEFFPGDDATIKVHVKKSKLYRNFTPGSHAFISFLSKNTFWQNHPFTAYESVTDPDTITFCCRVKKGVTRTIANKFKGSDAQSIQLKILVEGFYGEQSYYQQYDKSVFIAGNTGIVGPFSHIEKLVSSGSADRRIELYWGIRSYDALKWFGAELLSLKDKNVVPRVYISQPERSSINSSTDSTDSDHKNEEGDGILATEKKLSSPELETDLQQVLEFVEIIHGRMPVTEIIEAELKENTGTVAFGACAHTQVVDLVRMEIAKRLPSTCKRVDYFEEMQTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ33</accession>
    <name>A0A1E3NJ33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_153981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017267.1">
        <property type="nucleotide sequence ID" value="XM_019160808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ33"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ33"/>
    <dbReference type="GeneID" id="30177495"/>
    <dbReference type="OrthoDB" id="203678at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20220" checksum="7A50325B015CA15E" modified="2017-01-18" version="1">MEEQGTSEQRKSALDRIKEQRAALKNFYKLKEENEKEDKQAESDGEFGGSKIPLIHKNSSSFSIGGIDPSSIDDMEDFIANESYMNILKVENRVLDKLNSAKSEIKSTIYNNYYELIKINNVLGDLLRPNDSEVYSDTITDNLNTIRDNMQKIKSLDIDIFGDLEDQQARKNNETAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ45</accession>
    <name>A0A1E3NJ45_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_154124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017275.1">
        <property type="nucleotide sequence ID" value="XM_019160815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ45"/>
    <dbReference type="GeneID" id="30177502"/>
    <dbReference type="OrthoDB" id="3997966at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="164" mass="18543" checksum="A261A8DAFBE5DF05" modified="2017-01-18" version="1">MVLSSSARVPSFRKAGQRVHTQMQYRNFDPETNRPITALELLQKLEGNAELEVDILDITDVITKKEESRTQEQAQTSAHKDRDITSRMLDRYSKIAKTINKKSVKRRQAGTAANSTRTGSSGRTQIAEFEEYLSSKYPQAGVDTQKKLSLVLTQRIKELLGSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJK3</accession>
    <name>A0A1E3NJK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Required for respiratory growth protein 7, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72339</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017369.1">
        <property type="nucleotide sequence ID" value="XM_019164163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJK3"/>
    <dbReference type="GeneID" id="30180850"/>
    <dbReference type="OrthoDB" id="20734at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018828">
        <property type="entry name" value="RRG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133:SF1">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10356">
        <property type="entry name" value="RRG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="322" mass="36466" checksum="57803483FAD250DD" modified="2017-01-18" version="1">MLMLELLIFPKTSAKVPLSTGVGLTRIHHAIRINNLVKHSRMLNTKKPLTKQEGDITIEQKGERDSSSKDVKNKRPRGRPPKGKNAKQVVSPKLHGNSNLLDKFTDATSYLQYASYKLLKPTSSVFMGNLYELEVKNCLEQSFKIRSTLHQGGSNDKGIDINAIWDPTSVFHNENSDAPIEKKTKFEIVNSKKVKPVLQRSNQVLKLFVQCKCFDTSKIDPKLIREIKGSCQEYFKKNGNSAIFMVASTNGFTRTGKEDFDKAPIPLIYFKFSKPRLVNLKKPYDIRSWEMGNLGGLYLNPLAAALFKGLDWIKFTNKLLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJP2</accession>
    <name>A0A1E3NJP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elongation factor methyltransferase 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016676.1">
        <property type="nucleotide sequence ID" value="XM_019164375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJP2"/>
    <dbReference type="GeneID" id="30181062"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025784">
        <property type="entry name" value="EFM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF10">
        <property type="entry name" value="PROTEIN N-TERMINAL AND LYSINE N-METHYLTRANSFERASE EFM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51560">
        <property type="entry name" value="SAM_MT_NNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="28195" checksum="15804F3649FA675A" modified="2017-01-18" version="1">MTESMLPASLFAEPESFRPEQPQGTLAEYFTQTTDTGLQINLVGSSPLYGHILTHACLYLARYLEKHADTLVRNKTVLEVGSGGALPSILSAHLGARFVVSTDYPDADLIGNIRKNIAENKVGANCAALGYIWGNDTADIEATLRQHTAGECSTFDVIILSDVIFNHSEHRKLLHSCRSLLTPGTGKVLVTFSPHRPKLFHKDLEFFDLAQQAPFRFASRFVEMEHWSPLFKDDTDESTVELRSRVYFYMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK90</accession>
    <name>A0A1E3NK90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_25626</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017675.1">
        <property type="nucleotide sequence ID" value="XM_019162406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK90"/>
    <dbReference type="STRING" id="763406.A0A1E3NK90"/>
    <dbReference type="GeneID" id="30179093"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="60" mass="6702" checksum="FF9617D3899030EF" modified="2017-01-18" version="1" fragment="single">PICENCFTTTTPLWRKTSDNRLLCNACGLFFKLHGVIRPPTVNQQFKGNNNSNFNGNGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK99</accession>
    <name>A0A1E3NK99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_32836</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017685.1">
        <property type="nucleotide sequence ID" value="XM_019162787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK99"/>
    <dbReference type="STRING" id="763406.A0A1E3NK99"/>
    <dbReference type="GeneID" id="30179474"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF40">
        <property type="entry name" value="THIAMINE PATHWAY TRANSPORTER THI73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="32"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="476" mass="53969" checksum="D134E9C31F981B44" modified="2017-01-18" version="1">MSNLDSAENDQVSIIKSHKLSSKLKWKVDLMVMPFFMAIYFLQFLDKTLINYAAIMGLKKNLKGNEFSNLGTIFYVSYLATEPLAAYLIQKVHVGKFLGLNICCWGVVVACHAVTKTYASLMVIRVLLGIFEASVPACLVIIGGKWWTKPEQSRRTFFWYMQVGLATIVGSLMSFGFQHVHNTTIESWQIMFMFMGILTFVVGVLTFVFLPESPLKCRFLNDEEKEEVMQHIKENRTGFENKTYKFHQVKEMLMEKETWIIFFIIVFSLTDSGGLSTFSSQIMKTFGFSSKISALVQLPLGFVAIISTFLCCYLTSYVGERSIVMACVTVPTILGAALFIGLPNSYKVGKLFGVYLLNFNSAIIAQVYSWNAANTAGYTKRNARNAMTLMAFCIGNLIGPQLFQAKDAPEYVPAKIVLLVFLSLACVLSICLRFVVRHENKRRDRLTEGYTEEEKNKDVLLLDLTDRENLNFRYAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKC0</accession>
    <name>A0A1E3NKC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_77608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017682.1">
        <property type="nucleotide sequence ID" value="XM_019164957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKC0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKC0"/>
    <dbReference type="GeneID" id="30181644"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="32"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="271" mass="30319" checksum="A25F6596716CF805" modified="2017-01-18" version="1">MADPFSTFSVDEDFLDAPKFDNSYREREWILEHMAGAFRVLGSKDISEGLSGHISVKDPVDNNAFWINPLGLHYSMIKASDLVLYNENGDAIGGNPSGRISKPACQILAVVHKLRNAKVICHNHSPYGKAYSCFGKKLEMINQDVCFFYRVHNFYEAPKNVSGLEDIGRSIFERLGENGRALILQNHGLITIGSTVDEAAYLFLLLEKSCKIQLLVNSAANHGISKKLMDDAEAEANFKKSDSDSLYKAFQPDFQYQVSRDDSFLSFSKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKD4</accession>
    <name>A0A1E3NKD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell division control protein 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72637</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017693.1">
        <property type="nucleotide sequence ID" value="XM_019164256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKD4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKD4"/>
    <dbReference type="GeneID" id="30180943"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005825">
        <property type="term" value="C:half bridge of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000077">
        <property type="entry name" value="Centrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF48">
        <property type="entry name" value="CENTRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="158" mass="18405" checksum="786F6CF83F371AB8" modified="2017-01-18" version="1">MSLNKGVRQAKVELLNEQKQEIREAFTLFDMNNDGYLDYHELKVALRALGFDMPKKAVLDVIEEYDTNAKKLISYEDFYRYVAAKIVDRDPIDEIKRAFQLFDDDHTGKISLKNLRRVAKELGENMTDDELAAMIDEFDLDEDGEISQQEFINICLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKK2</accession>
    <name>A0A1E3NKK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_34979</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14 15">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14 15">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016976.1">
        <property type="nucleotide sequence ID" value="XM_019162915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKK2"/>
    <dbReference type="STRING" id="763406.A0A1E3NKK2"/>
    <dbReference type="GeneID" id="30179602"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040712">
        <property type="entry name" value="Pfk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF57">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18468">
        <property type="entry name" value="Pfk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 14 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase N-terminal" evidence="17">
        <location>
            <begin position="21"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="227"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="615"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="14">
        <location>
            <begin position="1"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interdomain linker" evidence="14">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="14">
        <location>
            <begin position="615"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="298"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="328"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="374"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="418"/>
            <end position="420"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="508"/>
            <end position="511"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="685"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="742"/>
            <end position="746"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="780"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="787"/>
            <end position="789"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="847"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="873"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="879"/>
            <end position="882"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="974"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ45863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1005" mass="110372" checksum="D823C96BB1AAAD67" modified="2017-01-18" version="1">MSAHVEAVSLDGKSDLASHVLYTSFAVISTSDYAALIQFYKKLGFRIVKTYSKDHYVSSSKTISGISTDSRKECWLESFPCSRTDTKGNLIPFQETIEYSHPELSSSQACMNRGVVLKVRLVSHDVHKNIELPGRIVLLTYSLDSVKKIAAKENLEIIQPSDAEKSVVSFFLKDPVGNLIGFTNKPLPDSGSTITSKDEYFTSKAVIDSLKEKEAEVDTRTKNSKKRIAVLTSGGDSQGMCAVVRAVVRAGIYLNCEVFGCYEGYSGLVQGGKLLKKLEWGDVRGWLSLGGTLIGTARCMEFREREGRLSAAKNMILRGIDALVVCGGDGSLTGADLFRSEWPDLVKELVTNGTFTDKQVEPYRNLTIVGLVGSIDNDMAMTDHTIGAFSSLERICEMVDFIDSTAASHSRAFVVEVMGRNCGWLALMAGICCGADYIFIPERPAKAESWKQSLKEICLRHRAKGRRKTTVIVAEGAIDDNLQKITSENVEECLVEMGLDTRITQLGHVQRGGTAVAFDRFLATVQGVEAVKAVLENTPSTPSPMIGILNNRVVRRPLMEAVKLTKSVATAISEKRFDDAMGLRDSTFKEYYSNFLSISQGDDNQNLLPESKRLKVAIIHVGAPTAALNAATRAAVLYLLSKGHEAYGIQNGFTGLIRHGAIRPLSWIDVEEWHNLGGSELGTNRSLPSLDMGAVAYHMQANHIQGLIVIGGFEGYQAVKELRDAREQYPILSMPIVCLPSTVSNNVPGTEYSIGSDTCLNVLVNYCDAVKQSASSSRRRVFVIEVQGGNCGYIAAFCGLVTGALAVYTPEVKVNLKSIQEDMDLLEKSYANDHGDERSGKIFIRNEKASKVYTTELVANIIKEAAHGRFESRTAIPGHVQQGKIPSSMDRISAVRFAIKACQFIEEDNEETAAKITNADFFKAEKDLRYTYKHGKKLEIAKGQNAVLCSIQGTSVCFKDIEKVWNEHTDVVGRKGLDMHWELLPVINDMLSGRLMIRENKPGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKQ0</accession>
    <name>A0A1E3NKQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72759</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017830.1">
        <property type="nucleotide sequence ID" value="XM_019164289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKQ0"/>
    <dbReference type="GeneID" id="30180976"/>
    <dbReference type="OrthoDB" id="10267950at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003877">
        <property type="term" value="F:ATP:ADP adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009163">
        <property type="entry name" value="Ap4A_phos1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043171">
        <property type="entry name" value="Ap4A_phos1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045759">
        <property type="entry name" value="Ap4A_phos1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019200">
        <property type="entry name" value="ATP_adenylylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_5G14690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19327">
        <property type="entry name" value="Ap4A_phos_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09830">
        <property type="entry name" value="ATP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000846">
        <property type="entry name" value="ATP_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ap4A phosphorylase 1/2 N-terminal" evidence="3">
        <location>
            <begin position="17"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="ATP adenylyltransferase C-terminal" evidence="2">
        <location>
            <begin position="225"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000846-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="350" mass="41528" checksum="0AD12BB789007880" modified="2017-01-18" version="1">MGEPLVIETLEELRDLDLFDRIVKIFDEAEKNGDLVFKEASDFERITDKKTKMEYEIKVVEALSKRPNNRASEPGDDVKVTQEKIADIQKKNPFLHPEPELTIIDSLFGNYRLILNKYPNTRYHFLLVTKEFEKQDTLLKPIELQLMHAILENLNRKIEEKNMTERYFSFFNSGPESGYSQFHKHIQFMMLPSNFHVYQDDVVRGVDYFIPKEIVENRRPLFHKKASFKHYILKLKDDPSDEEDEQDSLAILYMYLIKRALNIYKEHDMEDSMMSYNLLMMPDWIMIVPRRAAKYGEVWQNSLGFMGLFCVKNEELKNLVLELGFAKILEECGFPIEEDEEHIVYNEYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKR9</accession>
    <name>A0A1E3NKR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_58979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017858.1">
        <property type="nucleotide sequence ID" value="XM_019163332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKR9"/>
    <dbReference type="STRING" id="763406.A0A1E3NKR9"/>
    <dbReference type="GeneID" id="30180019"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="315"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="407" mass="46431" checksum="40B4C6B9606B28AE" modified="2017-01-18" version="1">MSTRPKRQVLNKLSSSPQGSYREDDQYGEGEDEDSIGLDEELEEEELSGEENLISDDEDEPMDDGELEADDVYDDGEAIYEGDEDEEDEEEEFEELARSKSRTKAKVPVESSKKEQNNVSVKNKSTNSLLISMNFVDKKKLSTVLSQPSSPGAKGTKPNAKTNSRTRNIKITYTEPNEDDFGSDIDTAEEVKPTSIKRSSNAKGNNKGRGRQTAATLNDDDDDDDDDDDDDEDKDDEEDEVDDDEDLDLEENMDDNEESDVDFSKLSERQRSKVLGVSEEPEEMTPELYEGKKLPKSVLALMEGSSKKKALTEEEIQLKKAEAARKRKTFNMRKLEAEKKETLKKLLHRKIDKVDAKKQEEENERRRRNQLKRKELITHRALFSWLSKTETVDDKKTNVSLFSLQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKW6</accession>
    <name>A0A1E3NKW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017321.1">
        <property type="nucleotide sequence ID" value="XM_019161094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKW6"/>
    <dbReference type="STRING" id="763406.A0A1E3NKW6"/>
    <dbReference type="GeneID" id="30177781"/>
    <dbReference type="OrthoDB" id="1612078at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="2">
        <location>
            <begin position="199"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="806" mass="88919" checksum="B4FCE7526479755F" modified="2017-01-18" version="1" fragment="single">MHLHVLFFTEKSLQCGVANNKSCSATRNIAMKQGALHREWSLWNQIVLGQAAPQDTTQRKDVSGISAEGEKEGYEEDGAKEGYTEKAEGMYSPYVGERGQDAGEKGRLGAGPLALATKKKVMKFALLWIPVFVCIRYLLINDVFSFKPFEPCSMSKMQAVPLVDDEPVFSLHNVLDPLFGIEEVNATLPHEVIDIPLMPYSNVSSPVYSSKLLNHTFALSWNKPAKVQYTPPPSNITYNRIVLTLDTTVQGVQYDRLLHVYLADTEIWRSSTIEPAGKLSHSYSQKDVTLYSSLFKEEGNLLVQLDNLVTSKLTGAFNVTINALFFNDPEGSKEAKDHFGGSNNSYPTFVPLTPSTVGDHVPPIVYYPDSPLSNIKLPAVNYNTTKLALLVSTSGNAAEEFWFSNVVDQYKDYFLAHNRHFYGHGSCRVINVYVNGIRVHSTNPKPYVFSGGVSPTLWNPIVSTGSFDLVPYFVDLTPILPLLWESPSSVLDIEITNCIDDDEKTVVKSGIGSNWITSASLAVWEDETVDDSFGTLESIDNTTAIKSFAIAPPFSGILTQILKASYQNALLSNITYVYKDGTQATNVTSYESSANQTSLILITKFGDSQSDLSVLKSNLSVSQLDPLTFEPIKDFTMLTNNTLKNKLKFLPPVTSTEEDGVADTDISFTTNLTVKFNIGLYSGLDPMFEIKSKENGTANFTISSAGNHGTGTMLHNYTLSKADGSLYSRVALADNGTIVYDNITETVSSLQNEEETASTTILSYFSKIAELDWLTQEEINEIGYFLNDQETTELVNLLDNCPISEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKX1</accession>
    <name>A0A1E3NKX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD-dependent protein deacetylase</fullName>
            <ecNumber evidence="6">2.3.1.286</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_72824</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="6"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017900.1">
        <property type="nucleotide sequence ID" value="XM_019164305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKX1"/>
    <dbReference type="GeneID" id="30180992"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099115">
        <property type="term" value="C:chromosome, subtelomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046970">
        <property type="term" value="F:histone H4K16 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045950">
        <property type="term" value="P:negative regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01408">
        <property type="entry name" value="SIRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017328">
        <property type="entry name" value="Sirtuin_class_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF6">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037938">
        <property type="entry name" value="SIR2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="12">
        <location>
            <begin position="7"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 10">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="34"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="116"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="233"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="352" mass="39512" checksum="434FC23708192971" modified="2017-01-18" version="1">MGKGQNTETITKQLDTVVSQLQRKNAKVAFFVGAGISTNCGIPDFRSPKTGLYSNLRKLNLPYPEAVFDIDYFRKKPKAFYTLAEELFPGKFVPSKFHYFIKLCQDKGILKRCYTQNIDTLERIAGVSDDKVLEAHGSFCGNHCIECSEEMPREKLRAFMDQKKIPTCANCEGYVKPDIVFFGEALPDKLWEFWEEDIDDIDVAIVAGTSLAVYPFASLPAEVGKKANRILINREKCGDFDTNPRKSDIVLLKDCDIIAELLCEKLGWSEDLEALWLAGNKSVRGKPENEVEPSSSQKDKAAATATEIAEKVEAGLEYDKNEEKNKRDKVKNKENDEDVDVLAGVVKKISIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLA3</accession>
    <name>A0A1E3NLA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor TFIIIC triple barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_33339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018022.1">
        <property type="nucleotide sequence ID" value="XM_019162812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLA3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLA3"/>
    <dbReference type="GeneID" id="30179499"/>
    <dbReference type="OrthoDB" id="414418at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000034">
        <property type="entry name" value="Transcription factor TFIIIC subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF51">
        <property type="entry name" value="TRANSCRIPTION FACTOR TAU 55 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="32252" checksum="F59A5E570AECB7C1" modified="2017-01-18" version="1">MTLKTVYIVRHGYRSNWLPLEQQVAPPTGVDSDPPLAPHGVDQAKELAVFLSRDLAARQLPVPQMIFSSPFYRCVETIHPTAQALGLEIHPERGLGEWFKPNRQVVPVPVDHAMLHTFFETVPSDMDWLWDTVVPSVKGETEESIFQRCQTFWARFIPKFEQKHPDVECIVLVTHAATKIALGMALAGYGSTREFLTEKDGGDGKTTRIGGSTCSVDAYRRNVAHNTWNMFMNAETSFLSLGPEMDWHFATSQFEAGSKEDVEYRARLQQEQSQANSRHARQCKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLQ0</accession>
    <name>A0A1E3NLQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_24199</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017386.1">
        <property type="nucleotide sequence ID" value="XM_019162342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NLQ0"/>
    <dbReference type="GeneID" id="30179029"/>
    <dbReference type="OrthoDB" id="369569at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="120" mass="13972" checksum="32915BC00B1664F3" modified="2017-01-18" version="1" fragment="single">QNHELKEFKKKKMWELATSPSKSIIMNIGMSYMSPNDIQVIPIMMLFMLFINTFKEMFVVNVKFKQLDSSIESEIDSYDVSIMKLIYILSCCGNLLVGLWKLYSMGLVPNKSSDWLGWEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLR9</accession>
    <name>A0A1E3NLR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018184.1">
        <property type="nucleotide sequence ID" value="XM_019160650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLR9"/>
    <dbReference type="GeneID" id="30177337"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF18">
        <property type="entry name" value="IPT_TIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="254" mass="27369" checksum="7C0F16F64B028C45" modified="2017-01-18" version="1">MTLADVALGAPSRKRKVGSVKFLSEITSSCNNNSNNNSSGIGKAAIVIKPILKQAHSVRNRLKGEGDKKVVVFTEAVAELSPSTTPTATTPGTAVDSTENVENTENVPGVSMQSVDKLFLASHSVFDPTSIDSLDHSYHTRASGNAGSTNSSTGSNLSLLLDYDSISNSPENFTAGNNNNNNNNNNNNNATLASIDSITMTGTKTNSALVYDINMYKNEIKHIKLTIKDLKRQKKCLTRNQHRVSLCKLFSRQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLV3</accession>
    <name>A0A1E3NLV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase subunit beta</fullName>
            <ecNumber evidence="15">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_31086</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="15"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018228.1">
        <property type="nucleotide sequence ID" value="XM_019162676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLV3"/>
    <dbReference type="GeneID" id="30179363"/>
    <dbReference type="OrthoDB" id="14523at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000004">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15" id="KW-0139">CF(1)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="173"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ47115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="502" mass="53677" checksum="DAB59B34E64022AC" modified="2017-01-18" version="1">MVARLFATSTRSALKAAKANLALNRGLATAAPAVGKVKSVIGAVVDVQFEQGQLPPILNALIMENNGSKLVLEVAQHLGENTVRTIAMDGTEGLVRGQAVNDTGAPISVPVGRGTLGRILNVIGDPVDERGPVECKERRPIHQDPPSFVDQSTTAEVLETGIKVVDLLAPYARGGKIGLFGGAGVGKTVFIQELINNVAKAHGGFSVFTGVGERTREGNDLYREMKETGVINLEGESKVALVFGQMNEPPGARARVALTGLTIAEYFRDEEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGLLQERITTTKKGSVTSVQAVYVPADDLTDPAPATTFAHLDATTVLSRGISELGIYPAVDPLDSKSRLLDASVVGQEHYDVATQVQETLQAYKSLQDIIAILGMDELSEQDKLTVERARKIQRFLSQPFSVAEVFTGIPGKLVRLEETVKSFRDVLGGKYDHLPENAFYMVGGIEDVIAKAEKLAAEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLV8</accession>
    <name>A0A1E3NLV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="5">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_15116</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018232.1">
        <property type="nucleotide sequence ID" value="XM_019160676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLV8"/>
    <dbReference type="GeneID" id="30177363"/>
    <dbReference type="OrthoDB" id="10263291at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="8">
        <location>
            <begin position="174"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="281" mass="29094" checksum="0D4724A13E21F31C" modified="2017-01-18" version="1">MSLPENYTLTILGAGVMGSAVLSAILKSKPSPFPSHIYCCTGSSKSADRLQSTYGDAISTSYGTNNAEAVRKADVIILGCKPFLCQAVIDSVKDALNDKQIVISLLAGWTIDQLSSAMGNPNIARVMTNTPAKYGCGTAIVSFAPVLQGSESVAVRDAVMALVDTVGMALELPEKNMDAATSLVGSGPAFVLLMMQALAEGGVRMGIPYEVAKKCAAKVMEGTAKMVQESGEHPEALKSMVCTPGGTTIGGLMVLEDKGVRGAISRSVEEAADIAAKLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM53</accession>
    <name>A0A1E3NM53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018338.1">
        <property type="nucleotide sequence ID" value="XM_019163890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM53"/>
    <dbReference type="STRING" id="763406.A0A1E3NM53"/>
    <dbReference type="GeneID" id="30180577"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF25">
        <property type="entry name" value="F-BAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="150"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="473"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="645"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="723"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="480"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="537"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="664"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="731"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="757"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="780"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="809" mass="89474" checksum="C7279DDC37C9068E" modified="2017-01-18" version="1">MSTVAFASKSPYPMKQKDAPPHAGKDKEKENRRRRSLSAFTLHSNTSFDHPKRRSFTSSRRSFSATFRLNEASSAAPHSSTGPDSGSHASSPVHTRDTDKLNLKTHTAAGKSRPASRFFSSPQFSSSTPNLTPKVAKYIDSELRVSHRPVQLDGVSLIIPTIVYKCCDFIRCNDPVEGLFRMNGSIKQVNLIESELQKNVDACEFADDPVTTVHDVAVVLKRWISKLDDGLITADVCNSLNQAHKELMLPDYPSEFEDADADADGELFLEAEDSLENIDQKLINMDQSFTLHSSPTKSTKSDIKSVLSTAAAAVSPLTRPRTTKPPTDTTITPTSTTTTATPTFKDHLVLDSCYASSLSKLPIENLHLLLYLLSFLHYLAKPEISDITKMHTSNLAKVFQLNFFKSIDLTVGTKSFSAEDLKTSYLMNEELLASMIQNSETIVRDLTFFVNNHRDEMDHLLNVKPEQKQSNIALAKNRKSSSASTKTTNTASSVSRNTSIDSGAPASNIRATELSTTLESCEEKEVTPVPRNVTKGSRLDDLERLEKANEEREVIRTPNRNITEDQSEHKAKKDTSYVHTGAHPDNSEDNTRGSAAPKKDLPKKELAKRRSFFGFLNKWKQSIPSSDAWRSSSYAQTRAISPKEIPIASQNSSSSLQTSKFEDSASSLPQMSMSEEATEHDSVPENRALTVPTAVAEENSDSLQKNAVDSHWAINRGIETSNNKVKYDSQPSVSASTAADSSTATPPVTAVEEEKSEDIKEQLQRLDPSEENGQAIIIPFKRDKPKSSKANTEKIAQKRFSLFKFKRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NML6</accession>
    <name>A0A1E3NML6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prephenate dehydrogenase [NADP(+)]</fullName>
            <shortName evidence="9">PRDH</shortName>
            <ecNumber evidence="9">1.3.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15329</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>prephenate + NADP(+) = 3-(4-hydroxyphenyl)pyruvate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21640"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Amino-acid biosynthesis; L-tyrosine biosynthesis; (4-hydroxyphenyl)pyruvate from prephenate (NADP(+) route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the prephenate/arogenate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.13" evidence="9"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018480.1">
        <property type="nucleotide sequence ID" value="XM_019160772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NML6"/>
    <dbReference type="STRING" id="763406.A0A1E3NML6"/>
    <dbReference type="GeneID" id="30177459"/>
    <dbReference type="OrthoDB" id="5399569at2759"/>
    <dbReference type="UniPathway" id="UPA00122">
        <property type="reaction ID" value="UER00962"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3660.10:FF:000002">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3660.10:FF:000004">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000339">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3660.10">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046825">
        <property type="entry name" value="PDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012385">
        <property type="entry name" value="Prephenate_DH_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20463">
        <property type="entry name" value="PDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036510">
        <property type="entry name" value="PDH_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="10">
        <location>
            <begin position="16"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="447" mass="50911" checksum="C675A5B285F0F68C" modified="2017-01-18" version="1">MTVLSKVDIEKLKQTKKIGIIGLGDMGLLYARRFSEAGWSVCGCDREERYQELKESMASLLPEVEIYRNGFYVSRTADYIIYSVEAENIEKIVALYGPATKVNAIVGGQTSCKSPEISSFEKHLPADVEIVSVHSLHGPRVDTTGQPLVLIQHRATQESFDFVESVMSCLRSKHVYLTYEEHDKITADTQAVTHAAFLSMGVAWHNSHQYPWETPKWTGGIENAKINISMRIFSNKWHVYAGLAITNPSAHKQISQYAISCEEIFTMMIQGRRDELRERILDAKQKVFGHLGKDHQLLLDDDLLQQYSLAKNPPGGHQRNSHLSLLSIVDSWSQLGIVPYDHIICSTPLFRIFLGVSEYLFCTPGLLEECINVAIEDITFRQDDLQFTMASREWANVVSYGDFNLYRKKFENTQVFFQHMFPEATKMGNEMIKTILQRVKDREAAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMQ9</accession>
    <name>A0A1E3NMQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_71479</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018520.1">
        <property type="nucleotide sequence ID" value="XM_019163937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMQ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMQ9"/>
    <dbReference type="GeneID" id="30180624"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="199"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="266"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="502" mass="55361" checksum="1EBEF112EF49D003" modified="2017-01-18" version="1">MAEPEVLPQERYAVWKKNTPLLYRQLQTSSLLWPSLTLDWLPDTTAPQKGDTTYTGHRLVLATFSAGANPLESVLFTALNLVDLHAAPCTTLQNYDYSPATGEFTYSLPTPRLDNTRELTERTKVNNSLGLLQRIPHLGDVHRVKHCPHNPDLLATASDSGAVRIFDRTRKPNNYNDSDFHRSDPASCAAAVETSDILLQYHTSETWTLDWNVQKPYTLATASNDGSIAVWNLDDQFKAPPRQKFSTLDSKFRFSTCTLTTPHHSVPAHNYGVNEVRWVPDHDSLLVSAGEDGSCKLWDVRSTSQSPSIEFPPLILADGTDALNTVDVDPFQTFNLISGTASGAVRFLDLRSPHQLPHEAHQTAHTGPITSSRRLHNMLKGSTKKFFIFVCIVLAMVATLGMTPATQRKKVLNPIKNLYNKKNSGNSAANAELDNQKDKSDSPKVIVVPDNAEKAPLNVEEDPEHPAYILPVVDAETKVVDDTEDTSVANAKAEAILDGAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMV5</accession>
    <name>A0A1E3NMV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_32265</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="12">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018529.1">
        <property type="nucleotide sequence ID" value="XM_019162753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMV5"/>
    <dbReference type="SMR" id="A0A1E3NMV5"/>
    <dbReference type="STRING" id="763406.A0A1E3NMV5"/>
    <dbReference type="GeneID" id="30179440"/>
    <dbReference type="OrthoDB" id="449280at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000001">
        <property type="entry name" value="Cytochrome c iso-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 10 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="13">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="110" mass="12111" checksum="E7490B2E89DE89B1" modified="2017-01-18" version="1">MPAPYEKGSAKKGATLFKTRCAQCHTIEKGGPNKVGPNLHGVMSRHSGQVEGYSYTEANKRAGVEWSEQTMSDYLENPKKYIPGTKMAFGGLKKPKDRNDLVTYIKEASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NND4</accession>
    <name>A0A1E3NND4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_31287</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018723.1">
        <property type="nucleotide sequence ID" value="XM_019162689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NND4"/>
    <dbReference type="STRING" id="763406.A0A1E3NND4"/>
    <dbReference type="GeneID" id="30179376"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF132">
        <property type="entry name" value="POLYAMINE TRANSPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="478"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="522"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="147"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="602" mass="66487" checksum="FCD432167406072C" modified="2017-01-18" version="1">MAPQAYDPEEATSQLSGNLSSTLSRADSLKMVRTETARSLKDKGLNKTVTLADPVRPTNVDNPIFPEEYTMETETGLVPVETLHEIGRSRAASRMLTRKSTRRSEASKPSNQEGYDKPFDPEIQFVTFTINDPANPHNWSPKARWFYTIVLSLMVVCVAFGSSIVTGALGLMSEKYHVSLEVSILSCSLMVLGFSVGPLLWSPLSEQIGRRPVYFISFGLYFIFNIPCAVAPNIGTILVCRFLCGVFAASGLANVGGSISDMFPTETRGKAIAYFAAAPYGGPVIGPLVGGFISRYDGRISLVFWVNFAFAGLMWIVGSAIPETYAPVILKKRAKKLRKDTGNPKIMTEQEAAGLSLKELIETCLYRPLKFAMTELVLDLMCFYVCLIYSLLYAFFFAYPVVFGELYNFGDDLVGMVLIPIIIGAGLALLTTPIIESYYLKTCQRRQATPEDRLIGAMIGAPFPAIALWILGATSEKHVFWVGPASSGLAFGYGMVLIYYSLNNYIIDTYAKYAASALATKVFLRSAGGAAFPLFTMQMYHKLGLHWASWLLAFITTAMIALPFAFYKWGKGLRKKFCRENYSAFDDDDDDEDENSTDGKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNT2</accession>
    <name>A0A1E3NNT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_32291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018873.1">
        <property type="nucleotide sequence ID" value="XM_019162755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNT2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNT2"/>
    <dbReference type="GeneID" id="30179442"/>
    <dbReference type="OrthoDB" id="275715at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20264">
        <property type="entry name" value="Complex1_LYR_LYRM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045297">
        <property type="entry name" value="Complex1_LYR_LYRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051522">
        <property type="entry name" value="ISC_assembly_LYR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13166:SF7">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13166">
        <property type="entry name" value="PROTEIN C6ORF149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="88" mass="10772" checksum="C5687B4BFA05C314" modified="2017-01-18" version="1">MAYAKTKVLTLYRSLLRNSYKFDHYNFREYFLRQTRSEFRKHRQVPDNEALVEDALKNLAVLKRQATINKMYHFDKLVVEKLDKHHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNW2</accession>
    <name>A0A1E3NNW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Very long-chain fatty acid transport protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Very-long-chain acyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_15680</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018894.1">
        <property type="nucleotide sequence ID" value="XM_019160921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNW2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNW2"/>
    <dbReference type="GeneID" id="30177608"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000019">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF15">
        <property type="entry name" value="FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="20">
        <location>
            <begin position="103"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ47781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="667" mass="76856" checksum="3F28B060CE9E4575" modified="2017-01-18" version="1">MWGQLCLLFGGIGFTLYVARKVKPGFWTNFNKKYRVKDDLYLFPLAIQFFRHYVTQSLITKKFSLWYEYEALSLGKNSDSQIAMKYPRQIKDFKEFDEQSFVVESLTYRELYNEVLRMSFILHHDYGIAKGDVVTMFFMNKPLFIIIWLALWNLGATPTFVNYNLTSNPLVHCIKVVESRIVLVDDECIDTFNKTRSQIENELPHLLIQIVDETSTTAQLKEPNRPTYRENDKIRDRGVRYWDPAVLVYTSGTTGLPKSAVNSWRKLFMATHLFPRSMHMDRSSNIYTAMPLYHGTASILGVLPALLVGGTISIGHKFSLSSYWTQVKVCDSNTIQYVGEVCRYLVDSKESVNEVECYGKIRLAYGNGLRPDIWMKLKKRFRIPAIGEFYSSSEAPFATTCYEYNGIGVGALRNNGYLLDKFLSLQYTLVRMDPEDESSIYRNPKTGLCENPGVNEKAEMIMRILNPKNIKTTFPGYVNDDAATYSKIIRNVFKKGDAWVRSDDLMRKDELGCIYFVDRMGDTYRWKSENVSTTEVENQIMEFVPDIQSCVIVGARVENHEGRAGYLLVQTVNNNTENEAQRLKILDDIYETGQKHLPHFARPVFIVFENISFTDNHKISKKLFRDPVLPNGKNGNLKVYYLDSQSKGYRPLDSVTYAQIANGKIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP32</accession>
    <name>A0A1E3NP32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_120141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018969.1">
        <property type="nucleotide sequence ID" value="XM_019159868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP32"/>
    <dbReference type="GeneID" id="30176555"/>
    <dbReference type="OrthoDB" id="3991441at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="97" mass="10985" checksum="12B5FBE2876E172A" modified="2017-01-18" version="1">MESTRIEYIKENIYIPPQVQPERPAIPARHPSLILNDKISDASVTFQCGCKGGKCIKETADWLYGPNGEINNPHDDCTKTEAYHFKNLMHDSVGRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP68</accession>
    <name>A0A1E3NP68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019003.1">
        <property type="nucleotide sequence ID" value="XM_019160953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP68"/>
    <dbReference type="STRING" id="763406.A0A1E3NP68"/>
    <dbReference type="GeneID" id="30177640"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF279">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="454" mass="51127" checksum="CBB662D8354D5F5E" modified="2017-01-18" version="1">MSKYLPVANSTRSFWLSAEEDDIKGYRSSEELPKEMDTVIIGSGYSGTATAYYLLKGNPSNKSILMFEARNICNGATSRNGGHLKCDLYYGYEGFLRKYGEKDAASIHNFEYDHIRATKALVEEEKIDCNFVITRACDIHFDKSGMDKAISGYNSVSKNSYVNYMHDLQINFGDEAKTISKVEKSPVCLTYTTGHLWPYKLVISLLRICIARGLQVQANTPVLKTKRLNDGRYLVKTPRGDVITKKLIVATNGYTVSVEPGFENKIIPVKGVVSRITSADPSKRTPHLTNTYALRYSSEDYDYLINRGDGSVIVGGGKPVYGDNLDSFYNVVDDSTIIPKTKEFFTNYMKERYYTWKDFDEKLDCIWSGILGLTDDTLPYVGEMLKEKNKFIIAGFHGHGMPRVLLCAKALARVINGESTSLEVPKPFKLTEARMENTENETFASLKTPRKHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP96</accession>
    <name>A0A1E3NP96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_71902</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018998.1">
        <property type="nucleotide sequence ID" value="XM_019164043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP96"/>
    <dbReference type="STRING" id="763406.A0A1E3NP96"/>
    <dbReference type="GeneID" id="30180730"/>
    <dbReference type="OrthoDB" id="5327978at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="416"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="24"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="449"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="449"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="467"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="544" mass="60289" checksum="7EE7198BFC72D2E3" modified="2017-01-18" version="1">MVWNQLDPDRTHVAYACLGFFTVLFALCSSVVKEKLFFGEATVSAVFGLVIGPHCFGWFDPHAWGNPLYNTLEISRILLCIELVAVGIELPPRYVYHKRTPLCTLLFFAMVVGWLVFAAFVHWLVPGYSFAWGLLVSACVTATDPVLAQAIIGKSKFASKYVPLHLKRLLTAESACNDGLAVPFVYLALNIIVHSGNSSQIAKNFICITVLYECLFGCVLGASLGYASSWAVRLAKKYDLIDNESNIFFPVAVAFFCAGISPILGIDDLLASFCAGCGFVWDDWYVRRREDESFIDSLDVFLNISYFIYFGSIVPWEQFNNAALGLNAWRLVVLAIVFLTLRRFPAVLMHYKINPEIRSFKEAIFVGHFGPIGVSGIFACILAISDLEVDAQGVAHGPVVGDVPDNVKMAQLINSIFPLVSFLVVVSIIVHGSSAAFIVFSSHLKKKKKEHRKMQSRRKQGSANDTQDNDAEDDDDIGDDDNDDNDDDNDDNDDDDDQRNEDDTDEEDDYTEQSNASDRSSEPIIQPIHIGESLKMADLPSAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPC5</accession>
    <name>A0A1E3NPC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15822</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019067.1">
        <property type="nucleotide sequence ID" value="XM_019160979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPC5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPC5"/>
    <dbReference type="GeneID" id="30177666"/>
    <dbReference type="OrthoDB" id="2445133at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424:SF1">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23187">
        <property type="entry name" value="UBX7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="280"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="490"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="539"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="551" mass="62164" checksum="554BC7E573A7DBEA" modified="2017-01-18" version="1" fragment="single">MLDDSALAERNLAFNSDVMLSIQSSITLRKPLMLFIEDPASAAHSDAWLGRMIFNNPGLTEPTKQLLSEDFIRLKIVKNSADFNNLVLIIPSFQSAATPCIFFIYSGQVFDAIAGDFDMRLIEEKIKQIHHNLHSLKGPQPTATSPPPPSTTTAVQQVPLTPTDLPPNTNIDNLNLNENQSMGQVFAEVPNRNSTPVKQEKKSLKEESAELAALKYRENLLKQQRQAKLDRERILYLLDLDRKEQRNKLIEKEKLKQQELEANTVLPQHIRENFHNSKVQNSSTYSIQLKLLDGTTARHKFKSDEKLSNVRDFVLRTYPDYNSFPFYFFKTIDRITFGEADENKSLMSLNLNMATLILKPVEPEESQQVSTAADNTSSSTFGWLKNKMFSYLWSADKLLHETDPSQENRSRPSVNDGSYTSSMMTHPYMEEESDNDTIYHTPVLNSTTPSSSSLRPTMSSFNLYGSQGLIQPASTTSSTHDLQNLLSRNNDQREEAFSEPSNEDSPVSHIVSNGNSNQSESSVDRSLRTVRNAEDVDVNNGNSISLQFPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPI0</accession>
    <name>A0A1E3NPI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inner kinetochore subunit AME1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71997</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019112.1">
        <property type="nucleotide sequence ID" value="XM_019164068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPI0"/>
    <dbReference type="GeneID" id="30180755"/>
    <dbReference type="OrthoDB" id="3998077at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048743">
        <property type="entry name" value="AME1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20994">
        <property type="entry name" value="CENPU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner kinetochore subunit AME1" evidence="3">
        <location>
            <begin position="148"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="367" mass="42614" checksum="F75B8CBCD210542D" modified="2017-01-18" version="1">MERTKLEVGREAKREFRKRGSARRELDLDDLVLDLPPPLPPHKTVLSDADAERNDDGEAEKAADRLLETASPNKKPKDALGLEEETEFPHLPPPRVISEAERRVVEKHRELAARRLQQRLEEKQMVIREAKHVKHIKRTAAVRSKSIKLKYRRMPSNKARLIYTDVISQVIKDYLRAPSTVERYTSGVAGRLHETAKKKKRGRKSKKLQQTESSSDKEIKQRAKTALKEYYDRVNEYFTSLIDLQLSNALIRSDLEKINAEKDKLRMQIFELRKERGVVGLEITEVRDEFRRLNSHFSVQDKHYKQLITLQEEGSFSKQGEVLSQDKLIDEINYRLDKYGNMPNTTPSCLKSLRRINALLGELPSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPI5</accession>
    <name>A0A1E3NPI5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018741.1">
        <property type="nucleotide sequence ID" value="XM_019160871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPI5"/>
    <dbReference type="GeneID" id="30177558"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="148" mass="15434" checksum="DADC32D5F0BEEF75" modified="2017-01-18" version="1">MFVKGLERRDEFYSDLTPITNDIMTTGTLTSSPASEVTNNLASQATDSSEALLTDSDTSAFHLTSGFGAISSSTASVTVTTGGKDSSDQKTTSAKLTHVTGSSSTVSPGDYKHQVSSSYKNAAGYHGGNKKKTLIFGLLGSSFLFSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPK4</accession>
    <name>A0A1E3NPK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II transcription factor B subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RNA polymerase II transcription factor B 38 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">RNA polymerase II transcription factor B p38 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_15852</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019108.1">
        <property type="nucleotide sequence ID" value="XM_019160996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPK4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPK4"/>
    <dbReference type="GeneID" id="30177683"/>
    <dbReference type="OrthoDB" id="5963at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16573">
        <property type="entry name" value="RING-HC_TFB3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000037">
        <property type="entry name" value="Cdk-activating kinase assembly factor MAT1, centre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004575">
        <property type="entry name" value="MAT1/Tfb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00570">
        <property type="entry name" value="cdk7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17121">
        <property type="entry name" value="zf-C3HC4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="12"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="MAT1 centre" evidence="10">
        <location>
            <begin position="64"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="126"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="329" mass="39273" checksum="7BCDC7B2658C4EE0" modified="2017-01-18" version="1">MVELDWNNVPKDLCPVCKTDKYLSPEIEFKINPECYHKICDACVDRIFALGPSPCPYPNCDKILRRNKFKAQVFDDLNVERECDIRRRVLSVYNKKEGDFQTPEMYDKYLEEIEDIVYKLLNRLDIEETEERLRQYSIENKQSIELNNARREQEYEKFIKLQKLERQYKSERNRLNNEVINEEKKLKTLQKEQVIDQLQNAASDKDPKLILETIRNVTMEKTKKCRDQLQKLEEKYLRQKEAIRKNLNPNDIKNKNKSKIPFTPFNGDRLVELPFKFDGNGYKDPFFSKSVDDIQYKASGYKLDEYYQRSLNDAFTGLECYIADEKMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPW8</accession>
    <name>A0A1E3NPW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small EDRK-rich factor-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15943</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019218.1">
        <property type="nucleotide sequence ID" value="XM_019161029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPW8"/>
    <dbReference type="GeneID" id="30177716"/>
    <dbReference type="OrthoDB" id="18018at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="62" mass="7195" checksum="DF5E377C0147F3D4" modified="2017-01-18" version="1">MARGNQRDLARQKNLKKQQELAKSTKKGDYQKRKENDADILREKQRLADERKAAEAAANLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ55</accession>
    <name>A0A1E3NQ55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA cap guanine-N(7) methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mRNA (guanine-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">mRNA cap methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16041</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for methylating the 5'-cap structure of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67008"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.56" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019343.1">
        <property type="nucleotide sequence ID" value="XM_019161067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ55"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ55"/>
    <dbReference type="GeneID" id="30177754"/>
    <dbReference type="OrthoDB" id="10248867at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004482">
        <property type="term" value="F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004971">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039753">
        <property type="entry name" value="RG7MT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189:SF2">
        <property type="entry name" value="MRNA CAP GUANINE-N7 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189">
        <property type="entry name" value="MRNA GUANINE-7- METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03291">
        <property type="entry name" value="mRNA_G-N7_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51562">
        <property type="entry name" value="RNA_CAP0_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MRNA cap 0 methyltransferase" evidence="13">
        <location>
            <begin position="170"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="453" mass="50859" checksum="9BEC0FF195006692" modified="2017-01-18" version="1">MSDFYDGPSRYKKRRVVTHRGGGNRRRDESRDAGASSSGVGSGHVGKPGRAVPEKVLPKPVWMTDEEYKKQQGSIVRAIQPLRKGAEADRGGQNPAERGNETGHEGFSRSGAAEAGAGAGAGDGTPMASEDVNVKLKYLKTLQAQGKDEIVRDYYNTQTFHSRQSKRTESPIYRLRSFNNCIKYILINKYGKPNGTVLELGCGKGGDLAKWGMLGTRQFVGIDLSDESIREAIKRYRNGRYDFQAVFATGDAFNVELPEILKDFNPDEVSHLQFDNVSMQFCMHYAFSSEASVESMLANVSKSLRVGGMFVGTIPSSDFIKWKLKKLPQGEHRWGNSIYSVTFPDLDCFDREKGAFVQPFGNVYNYFLKDAVEDVPEYVVPFEKFRSMCEEHGLELRYKKNFFDMFNKEVGHFFNQLPGPLIQSLRLPGGKYGVANEEREACSFYLAFAFERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQE4</accession>
    <name>A0A1E3NQE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019414.1">
        <property type="nucleotide sequence ID" value="XM_019164139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQE4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQE4"/>
    <dbReference type="GeneID" id="30180826"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019637">
        <property type="term" value="P:organophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16025">
        <property type="entry name" value="PAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF33">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="558" mass="63924" checksum="F6584EA8E59F4BC5" modified="2017-01-18" version="1">MSSTKKPNFMVIVADDLGFTDTSAFGGEINTPNLQRLAERGLRFTDFHTASACSPTRSMLLSGTDNHIAGLGQMNEFAERIPERFGGKPGYEGYLNFKVAALPEILKDSGYYNFMSGKWHLGLSPEHWPDKSGFEKSFALLPGAGNHFKYFPKDKKGEYLNFLSPIFEENGKRLDPEVDIPEDYYSTDYFTDKFIDFLRLDDRKERPFFGFLTYTAPHWPYQAPDEIIAKYKGVYDDGPEELRQQRLTRAIKLGILPEGVEPHKVETQRAKAWKELNDKEKEVESRIMEIYAAMVEIMDTNIGRVLDEIKHLGEEENTFVLFMSDNGAEGMMMEALSTTAERFQYFVGNYYKNEFHNLGKKDSFVWYSDQWAQAATAPYYMYKMWASEGGIRCPLIVSYPDLIEKKNDGIVKPAFVTVMDILPTILNIAGIEHPAPVFQNRQVACVKGTPWTKYLQGKSDRVHDEDKVTGWELFAQQAIRKGRFKALFIPKPLGEDKWQLFDLSKDPGETSDLALVKPDLLKELVNHWSEYVAETGLVECADIFYETLKGEYEPRVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQH2</accession>
    <name>A0A1E3NQH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_69996</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat WDR3/UTP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019463.1">
        <property type="nucleotide sequence ID" value="XM_019163528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQH2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQH2"/>
    <dbReference type="GeneID" id="30180215"/>
    <dbReference type="OrthoDB" id="407922at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000157">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25172">
        <property type="entry name" value="Beta-prop_WDR3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="109"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="151"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="383"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="466"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="557"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="599"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="641"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="6">
        <location>
            <begin position="766"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="716"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="913" mass="103151" checksum="9915F1127C722EC9" modified="2017-01-18" version="1">MVKSYARYIQDSVFGVISTKSNLVNNSKSNTVYASALDSVIEWNYKTQTKISVICDTPTPGSLNASSLLPPPEITRLAYSPFANLLAIGSSNGDIKVWDISTSSLIIKFQGHKSAVTCLEFDTNGTSLISGSNDSTIIIWDLISESGLIKFKGHRNAITSIYYYNDNYLISTSKDGMIKIWDLKINQCVETHLAHNGESWSMCVFNHENKDMILTSGLNGECKLWTLDVHQDDGHKITEFGTFQKQSKNKAVDIKFDPFNNLILIANSDKLAEVFRLRSATEIQKAVSKRTKRLSEKGMTQEEIEESLENSKVSMLITPFTIIFTDSKITNFCPTDSSSKDLNLLINNANNTLNYWNIKLPENVKKHNNVNDKISNLKYSVERPGHRGDIRSVDINDDNTLLYTGSNNQIKIWNLKTLNCIRSIEKIGNVLCGKFLPGGSLLVVGTKSGELKLIDLSTSEVVHHIEAAHDDSIWSIEITSDGKTIITGSSDKKVKYWEIKINSLENKLHLSHSKTLELNESVLAVRVSSDSKYLSVSLMDNTVKVFFFDTLKFYLSLYGHKLPVLSIDISFDSKMIITCSADKNVRIWGLDFGDCHKSIFAHDDSIMNIKFFPQSHNFLTCSKDKYIKYWDGDKFDQIQKFAAHTGEVWGLSISSDSEFFVSVSHDFSIRVWNMDNDEVFIQEEREKELDELYENELLTSLEGDDITPRKKIGGDGEEAEEDIDEAETVRAKTMENLKASEKLIEALDMLGDEDNALLLAMKTTPSEHLFDTLCKIKPNQLEDSLLILPFSHTVILLKAISKWTKEGDLLQKNTSKLNVICRTLFFIVRSNHMELISQSDDVFKTEITSLKDQLRSMILKTTKDVGFNIQGLKFLQNRWSQDHSHIYTDDSGSQQLSTDATDKVRKRVYTTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQP9</accession>
    <name>A0A1E3NQP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14007</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019544.1">
        <property type="nucleotide sequence ID" value="XM_019160181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQP9"/>
    <dbReference type="STRING" id="763406.A0A1E3NQP9"/>
    <dbReference type="GeneID" id="30176868"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000009">
        <property type="entry name" value="T-complex protein 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="554" mass="60189" checksum="B00D295F2C79E935" modified="2017-01-18" version="1">MFSNARSETLFLGGEKLSGDDVRDQNVLATQAVANVVKSSLGPVGLDKMLVDDIGDVTVTNDGATILSLLDVQHPAGKILVELAHQQDREVGDGTTSVVIIAAELLKRANELVKNKLHPTTIITGYRLALREAIRFINEVLSQSVESLGKETLINIAKTSMSSKIIGSDSNFFGEMVVNTMLSVKTMNNAGELKYPVKAVNILKAHGKSATESVLVDGYALNCTVASQAMLKKVTNAKIACLDINLQKARMAMGVQINIDDPEQLEEIRKREYGIVLERVKMIIDSGANVVLTTKGIDDLCLKEFVEHKVMAVRRCKKEDLKRIARATGATLVGNMSNMEGDETFESSYLGYAEEVVQEKFSDDECILVKRPKKHSAASIVLRGPNDYSLDEMERSIHDSLCVVKRTLESGQVVPGGGAVECALNIFLENFATTVGSREQLAIAEFATALLVIPKTLAVNAAKDASDLIAKLRSYHAASQQVSDADVKRKNYKNYGLDLIKGKVVNEVTSGVLEPTVSKIKSLKSALEACIAILRIDTMINVDPEPPKEDPHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQZ4</accession>
    <name>A0A1E3NQZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein bS6m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019608.1">
        <property type="nucleotide sequence ID" value="XM_019163564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQZ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQZ4"/>
    <dbReference type="GeneID" id="30180251"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15465">
        <property type="entry name" value="bS6_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000007">
        <property type="entry name" value="37S ribosomal protein Mrp17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_bS6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="Ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="122" mass="13488" checksum="B666DB4528C55A6C" modified="2017-01-18" version="1">MLYELIAIARVSNPLQVHTEAKALASTIGKLVINNKGVVREVQSLGCRPLPKIMTKAGEQHFQGSHFLMLFDSSSAVQREIIRSLKNDPRVIRVNLLRVDDKTNLNPGSSYARAVQSMSQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR08</accession>
    <name>A0A1E3NR08_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_65953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019629.1">
        <property type="nucleotide sequence ID" value="XM_019163489.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NR08"/>
    <dbReference type="GeneID" id="30180176"/>
    <dbReference type="OrthoDB" id="271111at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029453">
        <property type="entry name" value="Rictor_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029451">
        <property type="entry name" value="RICTOR_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029452">
        <property type="entry name" value="RICTOR_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14663">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14666">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14664">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14668">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01307">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01308">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01310">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR N-terminal" evidence="5">
        <location>
            <begin position="130"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR middle" evidence="4">
        <location>
            <begin position="768"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR" evidence="6">
        <location>
            <begin position="1477"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1398"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1512"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1703"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1797"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1880"/>
            <end position="1899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1979"/>
            <end position="2003"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1404"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1428"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1444"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1512"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1544"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1566"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1582"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1703"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1797"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1857"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1979"/>
            <end position="1988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2003" mass="222700" checksum="561A3C97963FA471" modified="2017-01-18" version="1">MALDIRDYLAKLLQKNILLKQGAVKLLNTPSITHSARYQAQQEFQEAQEQIVQLEKQLELLERLPQFSRKAGIPSAKKPAKNSETSTTYLTPKAVTPDVLNITGSATSAQNRLDTMIEALRFENDPRRQVELTNQICSLLISSETKNVRYPSMSVIIPIIWPMLMSKHKPVSACGYKLLRYFMTSHDDVVIVFYLYHKYHHHDRYYYFPYYDYSKIHNQQYINDTETDLYDFFNYSDLYKPESHYNTSERNDFNIKARETRTVSSKLTLPLVSDNTVKKWWPLIFLIVSTLVDDKGTDVSECIKFIRKVIELKGIRFLGSLSIRVSVALAESVIASISLKLNNFQNFTPSTLESIKLSINLVECLCEVVVADSKLAFETKVIKFLIQILIDPPFTVTHHQLTNMANSSASSASEITMTINMINNAVKNISTIPLPVIINLLRIPNTRKYLVDCGFIPLLLSALLDPPILAIANTTISPSHGHMNSPKMQHLASILSVVMCTWSGLGLLLENDARYLRVLIDGLYSEQVYVRTVVMSVIAGGLRIRKLTALTGNHDSYELWDWERAFIAANGSSKTKETVNPNDLNSLNVMKNFSLADIRTKLGGRCCPEYEDNIVNQFTAIFLQSCLQCGLIDVLKTLFNSFENDKRATRWIALLLSEILYLRSKIIPSQIEFDYTISLKMNRLIERESRKHAKLNQKVKDEININTNQLIKKSEIAANGSELAIVSIFLKTTTISSLHPFINLPASSAPLLDQVTLDNILQDLKNIPDDVDIRPIIVQTHLPATKEYREWNWALISVLVRGILWNDAKFEETLKTTKFFKRLISFYKPQKAGFVQVVYYSTGPIPGFTDRRQPLDVDFFLNRGKDSKDRDRDRRISRKQREKMAEMYVDVGIALIQLLLSKPEGAEILRASGIIQTVSTSLLRVSGVFINGPGNEPGKNWEADNVPDSGGSGNRNFAGFSLDHHDSMSLFNPNEATTAAGAASNDMHVSKSGSGIGESSASASTASTTNAISGSSGGTGISGSFGFFDSPGDNDTIIDDSNILKMKKEGLFGPTRLFSTLAWGYIRFIGVLSSHPVGIVIMEEYGVVDALFRLVQTGRDNFTSMKHSNDQNQNIDMIRFIIQEIDFRISGQLRFLIEKVAAVGDEDVRVEVVERIIGELCSLKKVNDNNDYTIERWCVGLLVRQSYDFSLKVSQLAVKGLIKYVEDERSVDILLSFNPDLGDINVNMNVRIGNKMYGGVLLWKVLGYERGVDYVNSRSSGLIEQIVEGWADSRREWVNKLETTTYKKMRGIVIEEEERYDYFELFQRLVKTEKGVQCIVNSGIIQHFVRVILIYCKFLKMGIEKELYGLGKRRRYAGKTERVKKGRKSFAGSKPGFIQSVEDRKKLIGKRPVWYDSSDTDYEDQSRRNSAEESHNSTHGGELRDADQEGEEPEETDREDDPEDSYKEDKEQQDEKAYIRRWEDKWLNDEADSQGLIEETKCSIWALGYLGQTDSGIFAIESVLENVIEEKNKARSRRRGEVRGRGRAKEPHTGDSSRHKYDDYSDCDEEEEEGESEGGSPERAKGAGGGDGDVGSSGEREYDAEESGSEDEEVLFDEDEHYENRKGWFADRSERYRDNKDIIENFIEIYKNSINWELRGSCITAIGKISECEMGCEILEESNIESIQRDERECDGRGIRVSLSKEGLIGYEQKKRVSEMIRRWKRENGAGGEGRRTEGDAGARGGGDAGVDDAGGDSNGGSHAVDYPGPNDNGHDASVDGAQAPGGGEPLREGPAAAEMGVDQIIRAVLHMTDLQDADEDDDDGGDDGDGDGDGADPAGDGANTVPGHDGDAPDTGPTHDPGHGLRQRLLSRSSADDGPGPGADVGPRNEAPARLLGDLRHAGDDAGGVGGGAARAAAPAETLGTEELRRRLRELCGGRALKRLRARARAGAGSVVGRVAVQVLFAVVAAGLVVLKSGVLRFLVAELELGSSGSAGAGTVGNRGCGRSGRRRRGRGEGGGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR98</accession>
    <name>A0A1E3NR98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="3">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_58486</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="3"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019321.1">
        <property type="nucleotide sequence ID" value="XM_019163304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR98"/>
    <dbReference type="STRING" id="763406.A0A1E3NR98"/>
    <dbReference type="GeneID" id="30179991"/>
    <dbReference type="OrthoDB" id="9995526at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="7">
        <location>
            <begin position="57"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="356"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="393" mass="44327" checksum="41382B109DF67A7D" modified="2017-01-18" version="1">MDGVFAVNKQSGATSRAVLDQLNRILSSSKVSQESLQKLQNTRKQAQGKQRIKKWKTTKLKMGHGGTLDPLASGVLVIGVGSGTKKLGDYTNGSVKKYECVGLLGGSTTTGDSEGELLLKTEVDHITNDLLEQTKERMVGTLDQTPPIFSALKMDGKRLYEYAREGLPLPRQIKSREVKITDMDIKNDTLTSEHEYVFLKSEVDENGKTLAEQLANNPTLNDHPVPFSREWKEKHKNDNLLPLEMRIIKDSKMYEDEEYRAPLLHFDATVSSGTYIRSLLSDMGRAVGSSAYMVKLVRWKQAEWELNKNVFEMEDFIKYGEEIWAPVLEKVLSSRDGNVNVREEMEKSIKAHPEAVKLEAEKVEGEEEEEGKKKQKLELSEDYGEPSSKKLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRE6</accession>
    <name>A0A1E3NRE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable NADPH dehydrogenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Estrogen-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70314</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Oxidoreductase that binds mammalian estrogens with high affinity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019810.1">
        <property type="nucleotide sequence ID" value="XM_019163610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRE6"/>
    <dbReference type="STRING" id="763406.A0A1E3NRE6"/>
    <dbReference type="GeneID" id="30180297"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="398" mass="44285" checksum="9D9420D8B940395C" modified="2017-01-18" version="1">MSNNLASSNLFKPVKVGPVELKNRLVYAPTTRFRNTEDFVATDSMLEYYTERATDNGGLLIVEATFPAPEFGLYERAPMIKTAQQVKAFKKIVDSVHSKGSAMAVQLWNLGRTADPKVLKKHNLPFIAPSAVYFDEASEKAAKEADNELRAMTIPEIKDMVAEYAACAKRSIDEAGFDIIEIHGAHMYLLDQFLQASSNKRTDEYGGSIENRARFMLEVVDACIGAVGAERVAIRLSPYAQFQGALGNDSEINPIVTWGYVLSELERRAKQGNRLAYISVVEPRVSGILDNPDAKSINFKWPDLIWKGIIIRAGGFLDKAVISKLPEVVNANDHTLIAVGRYFTSNPDLVKRLKNNLELTPYDRTTFYKVGNSGYLDYKGYGEPMDHSKDQVMPKALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRF3</accession>
    <name>A0A1E3NRF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pre-mRNA-processing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14191</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNW family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019767.1">
        <property type="nucleotide sequence ID" value="XM_019160264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRF3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRF3"/>
    <dbReference type="GeneID" id="30176951"/>
    <dbReference type="OrthoDB" id="666364at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="6">
        <location>
            <begin position="57"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="349"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="167"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="389" mass="44264" checksum="06326201F25FFD03" modified="2017-01-18" version="1">MSDKKLQLYDYDSEKKLSETASKTKDALNKILSRKLHNDKVTTLASTSTNSDTSYLQYTSAQLANLTNDEGLNAPKRRIIKITDEKVDPMLPQKFRIRKAPDGPPADGFAPVLHDETSTEKLTKADQKKWQIAPSVSNWKNNQGFIIGIENRLQNSNTPNKLTEQDINKSTQRFSALSDALKNAEQKAKEDLKTRANWRKRLESEEIARTQERLGRLAEEARNSRKTAQETITDFSIVSANDNLSRKGQEEDSSLSDKLQRRAERRRRAEEELKSEKLSTKQKIRKLAKEQGREISERVVLGVSEALRKKQKESVYDSELYLRTSHSAKGKPTDDLYDKPLFSQETALGDIYRSRNMSGHKGLGSTNDTEPQSTSVNFVRDSDAEKKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRF7</accession>
    <name>A0A1E3NRF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_11063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019256.1">
        <property type="nucleotide sequence ID" value="XM_019159746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NRF7"/>
    <dbReference type="GeneID" id="30176433"/>
    <dbReference type="OrthoDB" id="10264062at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF502">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="403"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="771" mass="88653" checksum="846BDF533D86EBE8" modified="2017-01-18" version="1">MAMLVTPRRFPPGKGQVLYAKAKVYLHLTSAKKDNVAGYLTIVKPYPDSKNDELIISFIADTDLSTEDKSSLDYFDLYGLDGEKFDFYSSSSNDAMDSSVKGVSNFPKVSRFIDRPRLSSISSYSFGVVINQLFTVQVRPKTTNLWQGSIILHPKQEGDRLPALFFHDDESAGTKREQKLKSQTFNPFSENQFGGNSLYWGGDRFISCLKNYATLTESTLEPGMLLVNCTPEDALNFVPNILDSKGKTTEDYDLNAEIGQALTTAKWTVLTGLASITGFAKKKINNAIENNVLPKSIQRFVDRAEVKKITDDFDSANVYLAKWALSVQEEAEKSRKMIIGNDYYKELIRSELGDSFVELTPLEVSKATRKERINKEKWSSFFDSAGCLQYTVDEVLGEIFHSGLEMDVRKEAWLFLLGVYPWDTSAAEREQLAKTLENNYYEYKNNWMADLERQRNDAFWKDQKSRIYKDLKRTDREIDLYENKKIAGQTNTRGEDILDTNGNDDDDDDGDDDIDSNGNKSLFNNPNLVVLRDILFSYNEINQNLGYVQGMSDLLSPLYYVFQDEPTTFWAFCRFMDRMERNFLRDLGGMKSQMLTLTELVQFMLPDLYVHLEKCDSSNLFFFFRMLLVWFKREVTFYETMDLWEIMWTDYYSSQFILFFTLAILQKHSKIIIQTLKGFDGILRYINDLSGKLDINDLLTRSELLFLKFKQMIELIDRSNSGVGYHSVERKSTDGTTAAEPIKVSKELRGLLSREVVVQKEESRTSETPFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRH4</accession>
    <name>A0A1E3NRH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019276.1">
        <property type="nucleotide sequence ID" value="XM_019161042.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRH4"/>
    <dbReference type="GeneID" id="30177729"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="630"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="768"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="895"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1113"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1234"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1319"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="772"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="914"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="925"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1113"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1234"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1369" mass="151883" checksum="016CEF3EC9EB6580" modified="2017-01-18" version="1">MRSSPHRPQQQQQQQHRQTPQIRVSDLSHLHLTSMDDLVGESGLDDSNLNPKLKLKYYNKALVSYLAQQGLNMNPLKLSARQLKEKHTNVTSLFSHGNPINSAASSNSSIETFITGNGQILFLPFNPQRKKRRRGNIRDEYNDDDDHHDHDNDSEEDHDDVDAEADENDNDEQDDDNEGDERNMNPEADEPYAPEDLECSPRKRSFILVKANRKSRTARTQTNASASAIPNDQPSHAHNITNHTFSVIVKLNKKCNLNKMVKVNYHTHSTVKWIPPEYSRKEPIDERFRTSDMIDWDLNLTNPDCYIPFKESAPHTLNMNSDDTYGVSNVDTNTLDDSLESDSMSLDSINNGNLTPLKKVVDVKFSNEPTQEIKTFKSLNPKDYINEPPLLSSNENLVESNLFKGKNHEPTDSSSDSNESFLPGYYIFLLPVVYPLNTPESIQVPNSLLSHEFTIQIQKGTIVAPTLQAPQSAFLSTSPSHHYKYDDQNFEFEQRFTPSHTQNIIPPPIGTNSNSNNTHNSKSSFFKKIGIHKSTSSNSKLDSSISKHNSISPRGSFSSSHNTAVSINENLHSRTRSSSASQGNPIVNFLHQSSNTPTIYNYSYKLPAVRLPPSDATSTLNKSIYVNKVWNKALNYELLLPKKFTQLSPPSNVSVSPNDKFLHDNTFLLQMKLVPLVKNLQLKRIKINIVEKVTYTPKSPNSKDHKVPKSRTTDKTVTMLEIKTKDKSNNSNVNKPNAPLKTQIIKGCVNDNLLTFCYNNEKSSCFKESDSSHGLSNSGSMKSSSGFLHGSKKMSRFLDNSLSISHNPITGNNHNTNNNIPQNQDDIVITNPVKLQCPLNFVANDDSKFIQKVYEHLCTGTSDLNGLHDGSDYVDRGNDTMSIFSVNSAGNDKSDVFSDDDQAVSRSPVRRQRNFSFSSTSNNNLGTSARNNLSNPNWSSVQHEDKANSHTFLPDTTFSNLKVRHRLQISFRISRPDPKIKNSDGEFKLHHYEVIVDTPIVFVSPFCVTEALDLPSYEDAVRIRMFEVAKNSSKFAMSHMNENDSNYGSGYGYESSSSDENAVFSPCSPLMSDSNVSPMLYSTTDGANNSSAQVTNLLATSITSTMSSLPAYENRDPSFNRKRSDSGCPSLPGTTPVNASSLLSAAFSRSKECSTSLNSVLASDFSNLSLGNATGVSNRNTIDGVIGVDTEAPPPEYTEIGFNNIKDINKNVDLHISSTTSEFSTPSILAFRSESTTNGTDNIDLTSKESKEAQENKGDMSAIKNYVAATDRNSVYEKENLSKKNLSSSFVDSPSLSGSRTSLKSERVALLSGAESSGFKANARNSTVESEMSAGNSFDLGERDDLESIINMNMGQIPTLNLTPTQNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV9</accession>
    <name>A0A1E3NRV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_81917</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019541.1">
        <property type="nucleotide sequence ID" value="XM_019164993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV9"/>
    <dbReference type="GeneID" id="30181680"/>
    <dbReference type="OrthoDB" id="5555409at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein L9" evidence="4">
        <location>
            <begin position="34"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="205" mass="22434" checksum="F3EF0ECCC4D7F781" modified="2017-01-18" version="1">MFSRSSSYVYAASKAELKKTGVRYTAQSKKNRINVQLLKDFPGVGIKGQIVAVKPSAMSNKLHPFNGAVYMNYEGAEPAIPVVTKAAAAAALAALKNAQKESKKTKKVKLNPILKSELEKSKKTDNLLSMDDLLAIDLNALSKDNLDLVFSKLPKKLIMVKRSRDSVLSSPGEPLDAIDKLGTYYAEVTHEEREQPITIVVNSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS86</accession>
    <name>A0A1E3NS86_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_75628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020130.1">
        <property type="nucleotide sequence ID" value="XM_019164939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS86"/>
    <dbReference type="GeneID" id="30181626"/>
    <dbReference type="OrthoDB" id="29596at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="162" mass="19292" checksum="BA18D3ABAD1C3248" modified="2017-01-18" version="1">MMLEIDSKYWDFYSLNKYKEFEIPYKLKSKIMPLSNRLPISYPLIGHINVMMFFPKVSFENKFIKPMIIDKEDGKVLMIDIKGSFHSLIEKSNFIHNELIFDKSRVVVITSPNYSEGYPIAIVNSHMKEIFHGDMMGMDKRIYFYHMKVLLENLKTFKINFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS94</accession>
    <name>A0A1E3NS94_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Outer spore wall assembly protein SHE10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019566.1">
        <property type="nucleotide sequence ID" value="XM_019160190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS94"/>
    <dbReference type="STRING" id="763406.A0A1E3NS94"/>
    <dbReference type="GeneID" id="30176877"/>
    <dbReference type="OrthoDB" id="3260408at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133501" description="Outer spore wall assembly protein SHE10" evidence="3">
        <location>
            <begin position="26"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="71518" checksum="8998DAA2F1188983" modified="2017-01-18" version="1">MNIARFLRNLVIFFASLFVFQHVLRVCPLKSPLQYNSLSEDFDPVCIYTHKSYDLISPYLSSAYEFYEKSPIKPYVDAPLDAYVKPGFDVVSEHLDKLSSYVSAYREAHEQSVIDTVEYSSKSISGLITTIAEFINRTIFPVVRECTKIAISRTNIYSRKLRLYCYLKWNIYAKPFLLRGLDKFLASRYGEFLIQFERSEYFQMFEQTVAYIYRSMVVTFHYMSEKVNYIYEIILASRETEAYKTMQFKQAFLNDFAKYLPGSFNFKSSQSTTSSSTTTTTSISVSLSSPPLLSISVSSTTVSPSVSTGEKYESLLKNTISSASEDFVSQVNELAEEYKARLHNKLQPEMRALAENVNTGYDDIHDLLNRINQVKDETNPLYVSRQDYRDALAAKKSSIEVQAQEIEDKMVKETENFLDDVLKIRVSIIETLEEFADSTLNAYSAAIISNGDDWKEWKKYKDLKNSLIKFRDELIDKKPVDDLTGFLNKLKRDTALFANEAGSYMSILRAKANIEFQSREKEEKEAREEELARSVVSEERAEEEQSTLAEIQAAESTVYEYDDDEEEETLTITQTRYVTQTLDSDVETEATSVGSVSSEYIVNLVQEGSDEVKVLLEPVDDEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSC1</accession>
    <name>A0A1E3NSC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GAE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_70579</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020112.1">
        <property type="nucleotide sequence ID" value="XM_019163679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSC1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSC1"/>
    <dbReference type="GeneID" id="30180366"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016482">
        <property type="term" value="P:cytosolic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1164"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1186"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="8">
        <location>
            <begin position="939"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1244" mass="138810" checksum="505D9BDBA373E1DF" modified="2017-01-18" version="1">MNSTKVLQEPWQFMGLEQSQAPDLLSEEEYFAETSDDVSTAQFQIPKIYCSTKDKKFDLFDKFSYAQLIEFLVRSRIAQCHRADKSPGRVPKQGKRDRGVFYEWLKAHVDLHSKQCNDFITENENSLLRLKNKRQLLMFLNRRFEKTHHLRNDASKYTDRVSIFRSLVKGSTSTNANPVVLPVLLFSMLLTFVFVLMLFTLFFKTNTFERLFYSYPPYMHLIKTSKTFLLAMSVRNSKTIAAERAVIRKESAKIRSSFRDIHLDNDKRKRNIQKLLYLYILGEPTYFGQVECLKLLASPRFTDKRVGYMATMLILDESQDILTLLTNSLDIDMKSSNQFIVGLALCTLGNIASPELAKDLYPDVEKLLTSQQSYIKKKAALVAAKLVEKDPMLTEVYLPYISDLLNEKSHGVLLGTCCLIDSIYYHDETSRPQLKQLVPKLLAQIKLLTTTGYSPEYSVKGISDPFLTVSLMKSLRTILQHDNDSQYLESLNDLLTQVCANFENTKGAGCAILYETVKTIFAVDSDSSLKVLGINILSKFLIQKDNNIRYVALDTLLQVIEFEPLAVQRHRATIVGCLHDGDISIRRRALELTFGIMNQQNIKLLTKELLKYLTNEEDEELKTYITTQLSISCEKYNANLEWTLETMISMLNTAGNYCNDTILSSILAMIMQVGNKSLTKKILTDMITTLKAGLYDQYGLSLVTVWCLGEYADLVIANIDKEITEESIVELINSVLAVANYSTEQQKIQIKIYVLTSCLKLSTKIQKPSLIEKLRQIINRSTTDMNLEIQSRAIEYAKIFSEPISIKRGLLERMPAPSIRKHESLSLLNRSHNLVSGNGAGDGSSKTGDILLDLLGDDFASSNNAGGNVKKEKESAIDLLSDIFGGSSITNNAANPAEQKNGNILGLFNSQAAGNTTGGFNGTTIADSPSLASFTAKIAEPESIEGFNDGMIKVGFVEKSVGNGAANLEAIVKNVSTTGASISGISVLCAVPKHQKLQLSSISKTVLATQDFTVLDVKITGNPGSKVKIRVKLSYVANGSKVDRQFDFAGQSDSAMDFKNIQVQAQQYLTKIDADVKLTLAVNDHKPRSPQSTKHVSVLDTFEAQTKLPRSYAVLGAGALYFLMVFLNFGGIGQLLSNLAGFALPGYLSLKALKTRTSTDDTKLLTYWVVFAALQIVEFWSKTILYWIPAYFLFKTLFLLYIALPSTNGAELIYKSVLAPLTDRFVVADGPAADLMDKVGEKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSN9</accession>
    <name>A0A1E3NSN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Topoisomerase I damage affected protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_9215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019801.1">
        <property type="nucleotide sequence ID" value="XM_019165077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSN9"/>
    <dbReference type="GeneID" id="30181764"/>
    <dbReference type="OrthoDB" id="4036304at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="58896" checksum="2908AE696ACC16A9" modified="2017-01-18" version="1">MSQDRSPSPNYLTVSKTETSTNHRSPEKPHSNTTAANNNSTIFKTPDARKIRGNNGGLTRSLTVGSGSKSPVRRHLRTMSITSNTSPACNSTTNSARKGINIGISLVGPVPSGSNGTVNKEKDLTSKTPRRKLFPSKQIFVQSASPNLNELDEEQHVEMQTRENLEVNNNDITSIMNSSPTPLSPSKLDSIPQDELSENFRLLASKEMEVLEIKNQLKDLLQRKKDKEFELQQLKLKIEKQLMANLKQQNNDQYRHRFEQHTPKSPTSLQKSVVQPSSSYVDPLMDDSFFPVSSNSADDERSKRQSWFAKPLNFIQQFDSLIYKEFEKLQIPDDDELNVCKDTSVLDMSYTLDQSKSLKPKEINAPIKSLAGDGDASGTDVVQSMSQHLWSFVNDVKSNLLVEENEPVSQPPSESSVKSSSQLVNPKNRLRTASISKRQSYSQHNHKKRSSSNSLKNEAVHSGNNDKQDIRDEFTTERISSVVQSISKHDDSGSEIATEMKSGVPDKTLDKYIKDSDLWDSEPLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSR8</accession>
    <name>A0A1E3NSR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="4">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="4">MTA phosphorylase</shortName>
            <shortName evidence="4">MTAP</shortName>
            <shortName evidence="4">MTAPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MEU1</name>
        <name evidence="6" type="ORF">PICMEDRAFT_70714</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020262.1">
        <property type="nucleotide sequence ID" value="XM_019163718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSR8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSR8"/>
    <dbReference type="GeneID" id="30180405"/>
    <dbReference type="OrthoDB" id="431409at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000008">
        <property type="entry name" value="S-methyl-5'-thioadenosine phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF2">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 4" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="62"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="95"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="234"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="4">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="4">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="323" mass="35336" checksum="8159973AF783E559" modified="2017-01-18" version="1">MSLPHTFNEKVPLAIIGGTGLYKLDSLIPVAKVTVSTPWGHPSSPITISKTASGMPIAFLARHGQTHNLLPTDVPSQANIAALKSLGVKIIIAFSAVGSLQEHIRPRDFVIPNQIIDRTKGIRKSTFFEKGFVAHAGFGDPFDGKIGDIIAKTCNDVLVNEEGVHDGRHKLHSKNTDGDDLTLICMEGPAFSTRAESKLYKSWGGSVINMSCIPESKLAREAEIAYQMICMSTDYDAWREDAEPVTVETVVGNLKSNSRNAHLLSERLIDVLEPLIKSGEIGNDLQGTMKMAVSTSENGRDKDVLEKMHFLFPGYWPVNDQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NST4</accession>
    <name>A0A1E3NST4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="14">Aconitase</shortName>
            <ecNumber evidence="14">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_70725</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="14"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020274.1">
        <property type="nucleotide sequence ID" value="XM_019163724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NST4"/>
    <dbReference type="STRING" id="763406.A0A1E3NST4"/>
    <dbReference type="GeneID" id="30180411"/>
    <dbReference type="OrthoDB" id="2224430at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01584">
        <property type="entry name" value="AcnA_Mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="63"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="16">
        <location>
            <begin position="579"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ49161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="779" mass="85156" checksum="711DCF3DA8176D24" modified="2017-01-18" version="1">MLSARSISRIPRTRGLATVSGLTKDSKVHMNNHEDHTFINYKNNVKNLEIVRGRLNRPLTYAEKILYSHLDKPESQDIQRGVSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPATVHCDHLIQAQKSGPEDLARAISLNKEVYDFLGSACAKYNIGFWKPGSGIIHQIVLENYAFPGALLIGTDSHTPNAGGLGQLAIGVGGADAVDVLAGFPWELKAPKVIGVKLTGKMSGWTSPKDIILKLAGITTVKGGTGAIVEYFGDGVDTFSCTGMATICNMGAEIGATTSVFPFNDSMVKYLEATERSEIAEFAKLYKKEYLSADEGAEYDQVIEIDLNTLEPHVNGPFTPDLATPISKMKEVAPAQGWPVDVRVALIGSCTNSSYEDMSRAASIIKDAEDHGLSAKTLFTVTPGSEQVRATIERDGFLQTFRDFGGSVLANACGPCIGQWDRQDIKKGDKNTIVSSFNRNFTARNDGNPETRAFVASPEMVVAYAIAGDLRFNPITDKLKDANGKEFLLKPPHGEGLPAKGYDAGENTYQAPPADRSTVDVKIAPTSDRLQKLTPFKPWDGKDALNMPILIKAFGKTTTDHISMAGPWLKYRGHLQNISNNYMIGAINIVNKKANTIQNHYNGAWGGVPDTAGWYRDHGHKWVVIGDENFGEGSSREHAALEPRYLGGFAIITKSFARIHETNLKKQGLLALNFKDVKDYDKINPDDEIDIVGLKDFAPGKDLTMVVHPKEGKPWNCSLTHTFNQEQIEWFKSGSALNRMKEIEAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT21</accession>
    <name>A0A1E3NT21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019826.1">
        <property type="nucleotide sequence ID" value="XM_019160288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT21"/>
    <dbReference type="STRING" id="763406.A0A1E3NT21"/>
    <dbReference type="GeneID" id="30176975"/>
    <dbReference type="OrthoDB" id="3998249at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="273" mass="29110" checksum="1969E12F7CCFEE3F" modified="2017-01-18" version="1">MMMIAGKPQSIPLTHSQKSSHERQHDINLLNAPKKTEPNAESSPSTPPGQKQLKSKSRSKAKASSRAKQQPQQQQQEQEAEAETQPRSQSKSSSSKRNASSGKIVIPPRSVEVSFNTNGSRGSQRAMLKSTADDLKHSTQDIKNLLVASPSTDGSKPNSRLSGGSSPNSSNASNHKKGGKKSPKSPNSQYAKLNNAINKVARLPDGSEPDFNNSRNHSAPLSRSSSSTSARTSKPNSASSNSSTSTDGIRYAGSSFHAEPKAVTLPKPSFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT26</accession>
    <name>A0A1E3NT26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase, mitochondrial</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA-splicing protein SPL1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_70770</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Plays a role in both tRNA-processing and mitochondrial metabolism. Involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mitochondrial tRNAs and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) in cytoplasmic tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020323.1">
        <property type="nucleotide sequence ID" value="XM_019163732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT26"/>
    <dbReference type="STRING" id="763406.A0A1E3NT26"/>
    <dbReference type="GeneID" id="30180419"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070903">
        <property type="term" value="P:mitochondrial tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000002">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="15">
        <location>
            <begin position="57"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ49210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="454" mass="50249" checksum="8AAFEB8DAB29724E" modified="2017-01-18" version="1">MQALAQFRQRLNVVRFLKQSPRYLSTAVNDVVAPPAGSAVSLKNSKRDSTQYGSRPIYLDMQATTPVDPRVLDKMLEFYTGFYGNPHSATHAYGWETETEVEKARSYVAEVIGADPKEIIFTSGATESNNAALKGVARFYSKTKKHIITTQTEHKCVLDTCRHLQDEGFDITYLKVGQDGLIDLEELEKSIRKDTAIVSIMGVNNEIGVIQPLEDIGRICRKHKVFFHSDCAQAFGKIPIDVNKMNIDLMSISSHKVYGPMGIGALFVRRRPRVRLDPIINGGGQERGLRSGTLAPPLVAGFGEASRLMVKEFENDHAHITKLSNKLIKNLLALEHTQLNGSPIHRYCGCVNVSFAYVEGESLLMALKDIALSSGSACTSASLEPSYVLHALGADDALAHSSIRFGIGRFTTEDEIDYVSQAMKERVGFLREMSPLWEMVKDGIDLNSIEWAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTF2</accession>
    <name>A0A1E3NTF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14366</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019946.1">
        <property type="nucleotide sequence ID" value="XM_019160338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTF2"/>
    <dbReference type="STRING" id="763406.A0A1E3NTF2"/>
    <dbReference type="GeneID" id="30177025"/>
    <dbReference type="OrthoDB" id="10250725at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="10"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="448" mass="52722" checksum="749FCE6D7FA33225" modified="2017-01-18" version="1">MSTREISGQFHELDVIGRGSFGIIRKVLHKPSGRTFVRKEISYKSMSRKEEKQLSGEFEILQNLDHPNIVQVYGYEKLPNEKVLNIYMEYCDGGDISELIKKYRESKQYIPETLIWQIFTQTLLALYRCHYGIDIEPVSSMFKSTPEVKPPEVPGLQVVIHRDIKPENIFFMSDKYTVKLGDFGLAKCLAPHTHFTQTYVGTPYYMPPEVISEQSYNTVCDVWSLGCVMYELCALTPPFTAKTHAQLQEKIKSGVYNELPSHYSNRLKLCIYACLITDPQERADVNQLLQETSFKIYRREWELNQKELELVKREIELKRKDSLFHRQGEEMNLKENELLLKEKQLIKFDKVLEDERKKINAQNEETRRVMRTEFKLALEDKLNKMLSELPTDIQVMLKSRSKENRMPNANQYYYNAAQTIQPLTSSPQKFRGPRTFEDYNEKNLRYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTI5</accession>
    <name>A0A1E3NTI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase assembly protein COX16, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cytochrome c oxidase assembly protein cox16, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70468</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. May participate in merging the COX1 and COX2 assembly lines.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019986.1">
        <property type="nucleotide sequence ID" value="XM_019163646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTI5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTI5"/>
    <dbReference type="GeneID" id="30180333"/>
    <dbReference type="OrthoDB" id="5516033at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020164">
        <property type="entry name" value="Cyt_c_Oxase_assmbl_COX16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130:SF14">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX16 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14138">
        <property type="entry name" value="COX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="120" mass="14143" checksum="AE56F66BD90BF524" modified="2017-01-18" version="1">MGFGSRSFMGSKEKASYENSFVGKYQKLVKKNSFLYFGMPMMLSIALGSVLLTNFAALRYERRDEKVKEMSEDEALNMINNRRKVDINDEYYKLQGILEEHQDWEPKRVERLPGESENKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTP5</accession>
    <name>A0A1E3NTP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_21481</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020479.1">
        <property type="nucleotide sequence ID" value="XM_019162205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTP5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTP5"/>
    <dbReference type="GeneID" id="30178892"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="373" mass="41296" checksum="13BC50C70E745136" modified="2017-01-18" version="1" fragment="single">VAVEQLHPALLEKAEALKEELAGLEHKMAQGSEFNVDENKRYAQVTGVVTVYEEFVALHENYEALVDMAHGGDEGGLADEARAELQTAVPELVETTARLKARLLPQHPFADKAAILELRPGAGGHEANIFAADLLAMYVRYCQLRGWKHEVLSKTDHASGSGITEAALVVNHHGAYDRLRHEAGVHRVQRIPSTETKGRVHTSAAGVVVLPKMDDDGAAGAAARTFRPDELRIDVMRASGAGGQHVNTTESAVRIVHLPTGIIVSIQDERSQHKNKEKALTVLRARLAEKELREKQERERSARTGQVSSVDRSDKIRTYNFQQNRVTDHRCNYTLHDLEGCMQGTKLDLVIDQVEKREADDAAEELIRKLETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTY2</accession>
    <name>A0A1E3NTY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_57141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020126.1">
        <property type="nucleotide sequence ID" value="XM_019163217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTY2"/>
    <dbReference type="GeneID" id="30179904"/>
    <dbReference type="OrthoDB" id="73788at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="40698" checksum="7A1CA4FBF3B78A75" modified="2017-01-18" version="1">MDPEAIENTLSREAQTAAGPESVAHDEKTDELVTSLETGVDKAYSTIQDSTLSGFTKISGIVAERLPELQKQWKDVKLPQLSNVNVNVNELTKNLKFDEYVKKEDLDLLKQRGGELMSKAGENTTKVLDDLDSDLEKIENLTINYAQQLGGQIGTFFKSQVGQGAASPAADEGAKQVSAGAWNWSGWGKQLTGLIAGDSPEAVLSQEKKTELLFSLPKGISAGTRAESQVHELQSDSSIYLKAVESGEFEDYKVSDEQHAEAKELLSNDSLHLMAVYKKICDAKESEAKPLAKEESDKDEKTDTDKDADKGTNKVEQKKISDADFWKVYFGKKDQIMQDEKKRRELLEKSTTVGEDDDEEDFNWDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTZ3</accession>
    <name>A0A1E3NTZ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020261.1">
        <property type="nucleotide sequence ID" value="XM_019160459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTZ3"/>
    <dbReference type="GeneID" id="30177146"/>
    <dbReference type="OrthoDB" id="429932at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012578">
        <property type="entry name" value="Nucl_pore_cmplx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003:SF1">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08058">
        <property type="entry name" value="NPCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="531" mass="59267" checksum="1846DA42D5944FAA" modified="2017-01-18" version="1">MSFNVSSFFSPAEDTFVNKRSTNKSFSSKNPNMLSSLPATADGRNEPETDEVYERSVYPETSLAHNLSATPISPYVPIKSLVESPFKASHAHAHSTPLVGSSSSYYNSTNGIRTQTPLKQDPMNADSLLKMKIKAHQQQFQDNMNVMQAGFNLADKQVSEETYLNSAFKPRRSTADVSMTHLKDIGSRKPGDRIIPNGNISQGNTNGMKLPLEPLRMPGYNDNISKHMSDSKGNMSVLQNLDDPYVAEALGRIVNKELEMRKLLYGTLTFLIYRFLRSIIRLFVYTNPVLAKSLRALSKLMRDYSSSIQNERLASFVKWLSQLFTFESFLRVGSYIEYMMSFILGTMIVATLYRLLKPQDKCLDLPLTRAQRKILGLTTDSNDKSIEGQDSKEGDNVENDDDEEEAILKKLLSSSPQRIEKPTRVFMPGDQGIDDVMGSLNSLAIGNAMHNAANVTWSSGMIPKANSTSPGNMENIRTQLSQRNGSVIADRNYSDGRNSRDYLSPNGKYMFAVNNELRQQGGFGASNSSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU19</accession>
    <name>A0A1E3NU19_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_76048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020599.1">
        <property type="nucleotide sequence ID" value="XM_019164944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU19"/>
    <dbReference type="GeneID" id="30181631"/>
    <dbReference type="OrthoDB" id="4084298at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="588"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="611" mass="68734" checksum="039576B6E9F3ECD7" modified="2017-01-18" version="1">MSEPRQGMASNGGVGGAPLVPLSITPSVGESGEPGSGRDRTREMQEQLKSTTSSVPPIPPRVSTRNVSAQLRNSRTASLIIPVMASSPSDDGNEDDEKDDIVDLRVRVGDGRKEEAVPDSPALPMRPPRTPTRPSNEFQPETLDMYGMDRIKGSEFSPITERRSRKSFASSEHGNSTSGDAEEPFLVLKERYLIQSRKYQVDYVVNMLTKSNLTVSSSVYFKKLHSSNDYIEVLSEIRTGSFDTGNLPILYSLLLNSLKLKENSFQVRHDTFDNETEAKFDATIDGLSPLLRSTPQQKQQFKNDLMKVYSLLTVTNKMPSEEPEENKVQQTIEPNAFVAAFVYRCASHNILAQAVIVALLIDEFIVKPNERSREFHFLLLRSIEAVDQEKLLAESEKTNPVAYVMKLISSSKFWCNLVLLLFKNDISTANHPLGLTNVLHRFVIYGVNSLVEVIVNLRYELGIFQPADDSDAADAKKDNCDLLRFHREFLILNDSAASQLVSYSLEHLSTKNQELQATLVERQKAYNELLHNYKQLNEEKISVGGQTEKARGENATLSAEKRSLNAQVEQALGQFDVIRQTMEKNKRVQELNDSMSKEIDRLTKENQRLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU88</accession>
    <name>A0A1E3NU88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">malate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_70700</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020246.1">
        <property type="nucleotide sequence ID" value="XM_019163711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU88"/>
    <dbReference type="STRING" id="763406.A0A1E3NU88"/>
    <dbReference type="GeneID" id="30180398"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006735">
        <property type="term" value="P:NADH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000009">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="152"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ49133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="338" mass="36464" checksum="7096049AFFAC7B76" modified="2017-01-18" version="1">MVKATILGAAGGIGQPLSLLLRLNKKIDELSLYDIVNTVGVAADLSHIPTHQVIKGYAPKDKNDSKTIKVALKDSDIVIIPAGIPRKPGMTRDDLFKINAGIVKNLVHQAGLSCPDAFICVISNPVNSTVPIAVEELKRLGVYNPRKMFGITTLDNLRLEEFLSSALAGKVPASDLYGDAIAIGGHSGDSIVPLINGWGKNFGSKEEEYTKLVKRVQFGGDEVVKAKDGKGSATLSMATAAYRFVNNLLDAITAADGSTVKEVAFVKIDELKTKKVPLFISKHTQYFSLPIVIGKDGIELVEFPEYLSEPEVLMTKHAVEKVKLDVIKGFNFVHGSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV41</accession>
    <name>A0A1E3NV41_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_14872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020526.1">
        <property type="nucleotide sequence ID" value="XM_019160576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV41"/>
    <dbReference type="GeneID" id="30177263"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="52" mass="5559" checksum="4D3CCA5591B3C8BB" modified="2017-01-18" version="1">MFMRLQLLQGGRACPPLGSACTLQASCFPVLSRRTPSAAGRTSSIRSSTCVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDH7</accession>
    <name>A0A1E3NDH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015292.1">
        <property type="nucleotide sequence ID" value="XM_019164871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDH7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDH7"/>
    <dbReference type="GeneID" id="30181558"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051604">
        <property type="entry name" value="Ergot_Alk_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43162">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43162:SF1">
        <property type="entry name" value="PRESTALK A DIFFERENTIATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="293" mass="31898" checksum="7E1CF1EFE79B3093" modified="2017-01-18" version="1">MKVVLFGSRGNVTKPLTQILLSKGVDTTVITRSAENGKAIETDGAKAAVGSTEDVEFLTKTFQGADAVFILVPANLFLPNTLENMASVCTKACDAILSAGVPNVVYLSSVGAFNPKIGFHYQNEQILKRKLSGVPSVSLVRPTGFFTNFYHNIGTIKKDNAVYFNVERETVESYVHPADIAQACADLILTPPTDEKFKMIYVESDHAKAADIEKMIHDALNIDVKWVTVSDEAALQGATSIGLPKENAELLTNLYSKVYQGEMLDDIKKHGSVIGTHKLADFFKTDFAAQFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDM9</accession>
    <name>A0A1E3NDM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylglycerol/phosphatidylinositol transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_37438</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015319.1">
        <property type="nucleotide sequence ID" value="XM_019163086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDM9"/>
    <dbReference type="GeneID" id="30179773"/>
    <dbReference type="OrthoDB" id="6409159at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00917">
        <property type="entry name" value="PG-PI_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033917">
        <property type="entry name" value="ML_PG-PI_TP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="8">
        <location>
            <begin position="26"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="158" mass="16793" checksum="CCF809DAA4BD700F" modified="2017-01-18" version="1">MVSLSFLPYVSSLLPLTRPVPGNSPVELCDLSARHSLRLDSVDITPNPPERGANLTITASGLLHTAVADGAYVDVVVNYGYIKLLTQTYDLCEELPNVDMTCPVAEGAYTLTKEVAIPGEVPPGKYTVFARAYNKDDTMITCLTGSVEFSAAGAVAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDS4</accession>
    <name>A0A1E3NDS4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_65706</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015394.1">
        <property type="nucleotide sequence ID" value="XM_019163488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDS4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDS4"/>
    <dbReference type="GeneID" id="30180175"/>
    <dbReference type="OrthoDB" id="1742084at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF20">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX SUBUNIT RSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="9">
        <location>
            <begin position="96"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="184" mass="21174" checksum="F205E23AA762415C" modified="2017-01-18" version="1">MIENKDMADGITNKTTTTLMISMLMIRTSDDDSDEEVYEEDVPSKKRQRLFRGSTAIIKENGKKVTAISTGKPAPAAWVQRAYSMLDELEELTDVDGNNVAAVFMKLPSRKLYADYYKIIKHVVLLDQMRKQLDSKMLLTWDDFIGEAKQMITNADTYNEQGSWVIKCADLIDKRMNEFIDQWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDV4</accession>
    <name>A0A1E3NDV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_13501</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015407.1">
        <property type="nucleotide sequence ID" value="XM_019160069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDV4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDV4"/>
    <dbReference type="GeneID" id="30176756"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="646"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="506"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="666" mass="73533" checksum="5CFCF2CEDF25BE34" modified="2017-01-18" version="1">MKVAIDFLAAKLVCCLSWLLYPVQYVLTALFQSSLLLIDGMIFDGSHSGSGRKTGGSRDEKTALDADSLFPPTIKFIKNLTALDARTSRSARGGRKNADEEEQRQEAAPATAKQCSTASGSVFLPLTNARGELEFQLGHAAAEGATPLYHSVQQLTPNSDSVSPNSSSGDSVHAHGKAKANPAGVAFKSEFNNLSPVSSIKSNNSQESLNAAANSSDACVKDSLSDEDDCDDLEDDVPDSEKIHQCPHCDSKFRIRGYLTRHMKKHSKTKAYHCPFYDDSTESRCHQTGGFSRRDTFKTHLKARHFRYPPGVKSNKRTGMMGWCGICGEKFLNNEIWVERHIEMGLCPGLTDDYIKTLKIGKKKTGKHSKFLDVDNHENIYNNNNNSSSSNILNHNSQNNHYLLHQHLHTRNASPENIYGSTTASAIATNAALPSNSTAGFYMQSPSSVNSSPSPFLSNPSLHKQATLAHLQPTSHPLFNSISDTENHHTDSTADDLETLELLLKQQELTRIISSLKQKQEEAQNMQQLQSLQQLQQFQQQQSIQQLQQLQQLQQQQQQQQQMHVQHVQAPATSNTDSSPASIVSPATPMRLSQYSQGQPAESAYSSNANANANITTNQLQHFLLQQLQLQQLDLPVLKDEYEDEYPSLDGEFSNPSDLIRAGGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEF2</accession>
    <name>A0A1E3NEF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase family 15 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74751</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015634.1">
        <property type="nucleotide sequence ID" value="XM_019164859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEF2"/>
    <dbReference type="STRING" id="763406.A0A1E3NEF2"/>
    <dbReference type="GeneID" id="30181546"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="45171" checksum="88CCEBA4A3E3C31A" modified="2017-01-18" version="1">MPIVPMSQVKSRINRFAAIAIVCLLIFYFTSNVSPNNTIVTKDELINTKAKTAPKRPSKKVKATFVTLARNEDLYELLESIQSMEDRFNHKFKYDWVFLNDAPFSEEFIMETTKSISGKTKYGVIPYEHWSAPIWINAKKAEEVRMDMKEREIIYGDSESYRYMCRYESGFFYRHPLMDEYDYYWRVEPSVKFYCDVDYDVFQFMQDNDYEYGFTVALKEYIETIPTLWDTTKKFLKDHPQYLAKDNSLELISDDNGETYNLCHFWSNFEVGKLDFLRGKAYRSYFDALDKSGGFFYERWGDAPVHSIAAALFLDKDKLHFFDDIGYFHGPFTSCPLKEDVRIKGRCSCDPKEDFTFHPASCINQYYKFKGLKKPAELAKYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEI3</accession>
    <name>A0A1E3NEI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_17966</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015645.1">
        <property type="nucleotide sequence ID" value="XM_019161829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEI3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEI3"/>
    <dbReference type="GeneID" id="30178516"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000907">
        <property type="term" value="F:sulfonate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044273">
        <property type="term" value="P:sulfur compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="6"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="195" mass="22596" checksum="1A519C1FBB3E960B" modified="2017-01-18" version="1">MLETGLGGDTQFIDSVELYNRFSPLLKTKLEGLRVLHSSREQANGTALIGAIQRKHVIDSIHPVVRYHPVLKKKSLFVNSGFSRRFLGLKQEESDNLLSFLLDHSKTCLDAHIRLQWDENTVVIWDNRRVVHSATADWDATSTRHAFRITTMAERPVETEEEYESWSPEAEEERLKLKNYYLNLSPSEYYEATMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NES8</accession>
    <name>A0A1E3NES8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015744.1">
        <property type="nucleotide sequence ID" value="XM_019160040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NES8"/>
    <dbReference type="STRING" id="763406.A0A1E3NES8"/>
    <dbReference type="GeneID" id="30176727"/>
    <dbReference type="OrthoDB" id="6278596at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021773">
        <property type="entry name" value="TPC11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374">
        <property type="entry name" value="FOIE GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11817">
        <property type="entry name" value="Foie-gras_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 11" evidence="2">
        <location>
            <begin position="360"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1227" mass="141316" checksum="DEFFD6838D542E3B" modified="2017-01-18" version="1">METYHRSLTSVPAPLVVFQGISSELRPQPPKRRIRTLKEQAYAGPGKKQNYGLQLQIDRELDEVKNGCVERLVSLIEKCNVNDRLWWPNQDSSGQTSQKQQQSQKLEKSSLKQKQQLHQSRQRLMSSLSSGSVNSKGLWFNIKYADSKYEIVVNGTKSPLSPYNKEGDFYGSLLPLQWSEKYYSYIPSVFIAFYEIGADKRWDDLLVNEVNRVKLKFSNTMIKFVCILINVHANTTDSDRIQSIVSRINMNPNSLFVINGLSDEISKREQTVFIRKLMIGLRQYSNDFFNVQIQKLKRREIKNDQYSEKFFSARNLIKMALFEQVKGITDYSTKLLEYAYDRLLQSLRSIDHVSQRSQHAEARAWLDVMCMHIVRSCIVLGDANIAYRKFTFHVQQIRELDPRNFTFEWLSTQYTWLGELLENINEDVIPIDPIMMPTLGLKEIRYNSYNMPQNGFNFLQAAEYRGLALQHEETGGSDNRVMLLTAALDSFNLAKSGKFSRIESRVYILLGDVYYSKKNYSMAVNNYFAGLSNYKQESCIFIVGCVLQKILHCYVGLSQVREAWAVYIELCSINDSVWKKAKALDSTKIFLDLMKEKIISLDLVNDDSQSILMDELRNSDDADAEVLAANTNSGVPFVKEGVFDFDVGIKEYTNMMNEGVDLQVLIRHKGDNMISSLEINKLEIDLGENSEFKIIRVTNDGENYDANNVLENEENSKSVVLYELTCDSKDISESITCPLAFKTIDGRINRKCIILQIHVCSSVVGKFYVPRVRVNGTLNDVQFSTVINIAGLDSTSRPFLKWFVKDGKDQVLNNHYPTNSFEVLPKIPKIKCNLKYDRVGFNGRVFPVFLKFVNEDTDSPVKVHIKGDGVLNKKYDVAVKWGEDHTDKSFDSQAIMKPGESVTTSCVLEMPQISGLPMSTIEGESDDNEVFNCNIKFHIEYELLNEGVSIEVKKEARVKIVEMIEWHTELRADQHGEFPNLFIIDEKKMLDVGANMNIPVHTRKWLFTLNLRNVSFEQMNITSCKFVVRGPKDVTLTLTPEVEDNEDDGTILKSMGEKKLKVWLDVVCYERIIRSVPVDVQCVIDYYIEEMKDVVQHYRLEMYKANLPHVDPRMLVVVNEGGSKEELVVTYIMENPTDRIFQYQTNLNPVSGVEIVDYVKSMQLSVMPFMQEKIELVYRVDRQYQEGRAEIALPEFTLYDRQFQVFVKPNVADDRLRFVDGHLFLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF29</accession>
    <name>A0A1E3NF29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_18138</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015860.1">
        <property type="nucleotide sequence ID" value="XM_019161894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF29"/>
    <dbReference type="STRING" id="763406.A0A1E3NF29"/>
    <dbReference type="GeneID" id="30178581"/>
    <dbReference type="OrthoDB" id="274828at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="460" mass="51527" checksum="6E18CD00BA9495C9" modified="2017-01-18" version="1">MLSFRSVGQCRAFSTNSAVLAAAATRKSFNKKSSYDKSKNKKGSGNDKTFKASVLTKNYKKLALKADENLIKELPSIVAENKDTIKNSFVKYPSESIQKLHIAGSFKPDQYNELYSQPLTLFRAVEDSRLLEIVNRNGSSAESRYIVNGHSGIGKSTMLAHFQAFALAHNNSGAILFPISNADLLVDGSSDFKLNPLTKKYDQPMYSRTFMKKFVNLNAESLAKIPLSGEITPLTNMYKASSHKVSGTLLDLANHILKASPIESNTTFAFTVMLDELSKQDKLPVYMTIDNFSAFIQHGMTKYRDTENRRIYFQNFTIADTLLQYVSGEKSFKNGAIMVATHGNHRLQRNGTLDVLVGDKPIEDFAYAKFKDFDYKLANRLLQNNGLQKFNVSKFNLDECKSLVSHLLKFNLIHNEYDLENQLLSGNLDTVIQRIASQKYLVSGNGIPKLVMDSCILSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF75</accession>
    <name>A0A1E3NF75_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_18162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015887.1">
        <property type="nucleotide sequence ID" value="XM_019161898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF75"/>
    <dbReference type="STRING" id="763406.A0A1E3NF75"/>
    <dbReference type="GeneID" id="30178585"/>
    <dbReference type="OrthoDB" id="496at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22881">
        <property type="entry name" value="Mdv1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="393"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="435"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="489"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="582"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="605"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="308"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="738" mass="81359" checksum="AABD8784BD979D7E" modified="2017-01-18" version="1">MEGINTDIAKLATTASVLVNSAAANANINDMLQKSKTYASTFESRDNNPSTTIVGTANKGLMGLLVKHGDDIRVAHSLRRSNFTGNGKKKKDFNVLSNAMLNEIVDASESGICESGENSGAAGTSNGVLADEETSFSLFQGFSAVLPEVDETIQSIKNMSGKMVQGGDNEKNIKNSLKNNKLASSRSKKIEEITMEKIKTCQNQRVLLNFKSDINYHLDLLEIRKGLGKSEIAEIDAKIDRLYNLRKEIYDNVNYYEKSEIDLENKLLEIQDRLEFIKDIEVLNGENVGEMNGDGTLGEEISGADEYTNEMNNDVNDPNKSSSKIIFKPKNSKQKGKGKSKDKGNDSGILNDIEGDEYDDDYVTVYMEEDSRSYSRVTSSTRNLRPGDQLHTFQAHNEAINCLAFNEPFEKLVTCSMDNTVRVWDMNRYKCVGLLEGHNAYVDCVTVSDNLAFTGSIDATVKMWDLDYFNKSHDTYSEGESLTPLLNSFEGHVDAITALTYNNGELVTGSEDKTIRQWDLSTGHILQTIDVMWASSMANSTINFGSSSSSNAALGASSSNIVVNRDTQYPYLSCMQVFDAALASGGSDGIVRLWDLRSGEVIRQLFGHTGAVTTLQFDKTFSLITGSADRSLRIWDLRTGGILDSFSYDNPIKKIMFDDLKVTSVVENERGIHVYDRTEQRHWMLGGDVLSSSTETIQTDPAPAPAAASCANFVNDLQFSVNYLVEGRSDGVVGVWNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFC2</accession>
    <name>A0A1E3NFC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_73652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015954.1">
        <property type="nucleotide sequence ID" value="XM_019164543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFC2"/>
    <dbReference type="GeneID" id="30181230"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="123" mass="14535" checksum="21E6697AAD1F8892" modified="2017-01-18" version="1">MSEEQNILASKRFDIIEGLIVDHTKKCVVTPYKKEIADKIPLISYIKELYDDWETYPTGTVIRYECPHTKRIVQENTKTKVFRKPDEEMMKKYNLDERFLTNQDLFFAVHNSMSGIEADCVCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFI7</accession>
    <name>A0A1E3NFI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_20620</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016004.1">
        <property type="nucleotide sequence ID" value="XM_019162166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFI7"/>
    <dbReference type="STRING" id="763406.A0A1E3NFI7"/>
    <dbReference type="GeneID" id="30178853"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15485">
        <property type="entry name" value="ZIP_Cat8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF12">
        <property type="entry name" value="REGULATORY PROTEIN CAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="781"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="823"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="926" mass="104253" checksum="DD600EB5D1479182" modified="2017-01-18" version="1" fragment="single">PREPSSVKSKTIKPPGSNTTRVSVACDRCRKKKIRCFYDNDDPIVEDEDGNRQCANCKVVGLECIFTDKLARKAFPRGYTESLEERVRELEYENKKLQKLLSLKNTNKNSLISSSNSISTENTPTTVPSHVLNTENVSRLDVDFKPVLHTHDENCNCGMHHSVINRPVSIAGSVDIDQGDLSDDESLYSTDSHYSRNNDSDSKNRFVYNPNSFEQINAPGAAAAISLQNKLRTKNFLNLANLIAASIPRSTEETLFIPTLLARIIKTHGFDSKAPYLTARSIALLKQAYNEEERYNLFPITFKGVNFNDLNKSESKAFFQSLNLPNNMNLDICITIFFNTWNTVIPVMNKEIFMANYKKFIKSRDANYEDGELVGFEKFGELLVIITCLVMISNERNNNGKSSDKKISSNSEILQFYDHIIKQLIQSNMSSICSISSLQIITIELLYCLATDDLTTSYELRGRMITMCQQLRLHRCPAAVLGSNGSSVSKLQQGERRILFWCIYTLDSFSAFILGVPRLLKDYEIECALPSSSSSDKENKDSDISLITFQNNVQLSLVGKVCDSALSVMRYSKVLGTIVDSIFRRSEGLKQTNINESKCLIMEDLLETWRRNLPANVSFNTVHNSKDINIIDNLNETQLTLYYLYYQAKALIYMPLLAGESTFNLTSSKCSPAFISIQQATTSILTTMRVLNTSKHNFYFLPLPINIARQTARFSLMAARNSLEYTRGGSLFQEAKLLLTSIINELKIENKIGLLGCLSNNCVGCLEHAIDAILSQPKTGSMNNLDPVESVKKESKKKSTGSPLKKVSNLESFAKKPSSMAPQQFLQTQQENLQKQIQLLQQQAKTQAMAEAEIQVQRQQVEQQRLQNSLFQDSSKNSHADVPFSVKSFSDLLMFNDFGVDASLGLPFIDFELDNTQTKKQKMDPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFQ6</accession>
    <name>A0A1E3NFQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Biosynthesis of nicotinic acid protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BNA5</name>
        <name evidence="6" type="ORF">PICMEDRAFT_73769</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-kynurenine + H2O = anthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016083.1">
        <property type="nucleotide sequence ID" value="XM_019164581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFQ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFQ6"/>
    <dbReference type="GeneID" id="30181268"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000031">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084:SF0">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22580">
        <property type="entry name" value="KYNU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ44970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="467" mass="52433" checksum="C898086F9C0EAA32" modified="2017-01-18" version="1">MLEAWEELREEARVLDGTHETHADMFVFPTFETMGLCARNPELDPQTRVNYLCGNSLGLMPVQTRRYVEKELEAWGARAVESHFRNEITTDWVDVDLPLVPKLARLVGAKETEVAVMGTLTMDLNSLLVGFYRPDVASGRTKILFEKGAFPSDYYALYNLARLNGLAPEETLVQLAPREGEYTLRTEDILSSLEKDGDMIALVCFSGIQYYSGQYFDIKQITAKAHEKGCVVGWDLAHAAGNVDLKLHEWDIDFAAWCSYKYLNAGPGAIGGVFVHERHTGGDDRTGRARLAGWWGNDAANRFEMLEKFRPIETALGFRQSNPSVLDVSALNSSLDVFEACGGIGKLRAKSIQLTAFLERCLHKSRFYRSIEDSNKTQDAADVHFVIITPQDPAQRGSQLSLLFKPLEKDVMMQVFQFMNQRGSICDERKPNVIRLGPTALYNTFADCVECVQLLEEGLLSYTSDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFU0</accession>
    <name>A0A1E3NFU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016114.1">
        <property type="nucleotide sequence ID" value="XM_019161626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFU0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFU0"/>
    <dbReference type="GeneID" id="30178313"/>
    <dbReference type="OrthoDB" id="2150604at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="55931" checksum="F669FD07A529E22F" modified="2017-01-18" version="1">MQQQTYTREFTPFEKYYFWRNKLNMYSNFRVAVEYMEELDTGSVFQALSLMVSQHTSLSMNAYPKKGCTFFEFPYTLGLMDSYRLSDVLEIINDENVTVGDVFNQLQDVHFEYGTSKPLWSLILLNKKTLIYYSDHLLFDGSSGLNFHKLFSHAIEVSEKAENFDAEASILFDITKVDNDFQLPPKPSDLIDYSVPRFYLLYALMLICFPKSLSDIVKYYFIDDASISKSKTYNTIVLSRKMMSYNEDGPETNKTHNNIEKNRDRCKLVHISNTKLQQLICDCRKYNVKLTSLLVVMSLLSISKITGKEKDTMVVIPVNSRSKIDMNRVKMHGSPDIKTDFGLYLGDLNMELPPVDKMCHNGKINWNFVKYVNDYIHGRMKDSQRDLGLVSWVDPKLFLEYRKKCYDDSMIGTLLVSNLGNLKDTNNAFLNAYFDQPCRSGVLSLFAMNVISTTRGGAHLALRSVNQRWLEVFNEGVINQLSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG17</accession>
    <name>A0A1E3NG17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane proteolipid 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_37177</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015621.1">
        <property type="nucleotide sequence ID" value="XM_019163064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG17"/>
    <dbReference type="GeneID" id="30179751"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF42">
        <property type="entry name" value="UPF0057 MEMBRANE PROTEIN ZK632.10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01309">
        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133353" description="Plasma membrane proteolipid 3" evidence="7">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6479" checksum="D41803AC7AB954EC" modified="2017-01-18" version="1">MSKFLLYILALIFPPIAVYSLEGANKTFWVNLFLCICIWFPGILHALYIVNKSEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGB9</accession>
    <name>A0A1E3NGB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74635</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015506.1">
        <property type="nucleotide sequence ID" value="XM_019164826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGB9"/>
    <dbReference type="STRING" id="763406.A0A1E3NGB9"/>
    <dbReference type="GeneID" id="30181513"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000004">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF9">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="38"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="327" mass="36478" checksum="B9D6F3D93DAA5837" modified="2017-01-18" version="1">MSAENLPWVEKYRPQCLDDVYGQTETVGTIRKFAKEGRIPHLLFYGPPGSGKTSTIIALAKELYGKNYKNMVLELNASDDRGIDVVRDQIKNFASTRQIFTTGFKLIILDEADAMTNVAQNALRRVIEKFTKNTRFCILANYSNKLNPALLSRCTRFRFAPLSNDAVRERVEYVAKMEHLHIEAEAKDTLVSLSHGDMRKALNVLQACQSALDNPGTDEIDSDMIYDCIGAPKPRSIETIFDAIVKNDWADAFSTMIKIRRVEGLALIDLINSFVDVLDKYELKPATKAKLLQGLADIEYGISKGGNDNIQSTAVIGCIKDALECEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGG7</accession>
    <name>A0A1E3NGG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_73978</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS26 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016315.1">
        <property type="nucleotide sequence ID" value="XM_019164637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGG7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGG7"/>
    <dbReference type="GeneID" id="30181324"/>
    <dbReference type="OrthoDB" id="3821113at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170071">
        <property type="term" value="C:CROP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000015">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="37934" checksum="50C07929C9FF3382" modified="2017-01-18" version="1">MSFIFKSPLDVEINLDSEDTREVVEVNSSKGRKERLLLYKDGETVRGVAILRTRDNKKVEHNGIRVQLLGTIETHNEGKVTNDDFLSFAQELAAPGELKNTESFNFEFRNVEKMYESYRGTNVRLRYYLKVTVSRKSADIIKERELWVYQYVHSNGAAAKNGSSSDKLSSYSASTTSDKSIASKNSSGQVDKSPSAVSTVKMDVGIESCLHIEFEYSKNRFPLKGVIVGRIYFLLVRLKIKHMELSLIRRETVGSGASQVSESTTVVRFEIMDGAPVKGETIPIRLFLGGFDLVPTYRDVNKKFSTRTFLSLVLIDEDARRYFKQSEIILYREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGH1</accession>
    <name>A0A1E3NGH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_64868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016350.1">
        <property type="nucleotide sequence ID" value="XM_019163482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGH1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGH1"/>
    <dbReference type="GeneID" id="30180169"/>
    <dbReference type="OrthoDB" id="1749473at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="271"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="359"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="504"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="598"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="435"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="439"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="476"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="868" mass="99384" checksum="DC74F62EFB888905" modified="2017-01-18" version="1">MTSNDLNQATKVSDLSLDETHSFPKSLRYIKSKWLGKEDKRIDASSKENIQSETEGQSPVSRPATNHKTAANINFSTNPTTINLDVDSDLENLSKSVTPSPEKGKRRKAFHTIKSYLEKNKSQQSIETKQSKEPQKEDFSSVLVPDSTPSSNQLTIFEEKHHKTAENCSSKQDYSIDTIDTKLDQPKTLFEIFKVIENKKQFRKELADDRVNQSESDSELSSDVLTISKNKNEQNENISRDEFGHPSKVSNAKVNGSVLPIPMFNYQTKMISLYVGDLDKSVTETDLYMFFSKYEGLISVKIPLDTIKNESLSYGYVNFDNQAHADLATDGLNYTSLKGSDVRIMPSLRDKTQRENMGANLFFSKLSHDLSSRIMFDRLKVFGKILSCKYSKEKGTCFVNFADKLQAYKICKQFNETKMDDRIINVSVHISKKERESFQQHSKPVSYTSKELIPNSTSTNLDTSPSSDVDKKSENVRSGSTDLENENQGVEKDAATSKPISTQYSIFIRNMPLHLKRDVVRSLVEPYGTVKNILTRDVPIKQGSWALVTMTNKAAVDKTIQSLNSVELEGKQLFVTRAIPREEKEYAKREEKYPKKKLKLLVSQIDMEKDKESLENWCSKCGSIKSAEFYASSNDANQSNGYGYIELNDENDADNLVKEIRELGVSCYKIKIEIPSKENNFETFRYPYVMNALSRNDNYNEFRKNPGVVSFSYVDPNKMFQLANFQKTVDNDKISVFQRLENNNKTKFKERGNPLSETDEEILKEKQNEMYRVIWEICVRLFIPKPEIWANQKYCAGISPTNEILSKSKISSLTNHLVKFFWANNFNEFYKFMKQNQFDKKNRLLYVAHPVLANQIVQSATYLGIVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGL0</accession>
    <name>A0A1E3NGL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74416</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015821.1">
        <property type="nucleotide sequence ID" value="XM_019164764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGL0"/>
    <dbReference type="STRING" id="763406.A0A1E3NGL0"/>
    <dbReference type="GeneID" id="30181451"/>
    <dbReference type="OrthoDB" id="1716531at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="248"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="287" mass="33688" checksum="22C3112E2C044ADC" modified="2017-01-18" version="1">MEAMPFQWLQSIPPVTRTYVACSTALSLADYMGYIKVSDIFLSPNSSFGLNQLWRILLNTAYNGTFSFEFCTRLYLFTSYSNWVETYVNSPRHYIWTLFVLIFMINLYSAFIKNLGLIGPVLKDTLLCIWSRKQSDDEVFFFIISLKVRWLPWATYLLDSSINYREDPKRFIAGPAGLIIGHCFWFINEELPRLHGNKSFLRPVWEWEIFNLETIKPEENGLEETTETGQMDLAQVDLQDHQGENQLEENLEGHLREMPLQHAEVVDDHDHPNYGRETRDTLHQRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGQ5</accession>
    <name>A0A1E3NGQ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016430.1">
        <property type="nucleotide sequence ID" value="XM_019164671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGQ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGQ5"/>
    <dbReference type="GeneID" id="30181358"/>
    <dbReference type="OrthoDB" id="3986885at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012470">
        <property type="entry name" value="Pup1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07954">
        <property type="entry name" value="DUF1689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="220" mass="24794" checksum="5A700A3F8059B1AF" modified="2017-01-18" version="1">MGTDCAPCLADSEAPGAFMIDINRLFSTDKKDAVAFYKQEHDLTADERQELHQFFEGTYKTTTVAKSFVGIAAGLTMVWLARRKRKISPVYAMAGGIGISAFVFSYMTPTIYQSKLQELEKRFGKDSKVYKVVEVTPEGAEYSYYWSEYFNNSILDQTVRLKDPSKPPAADNDVEFVDANVSFGHRSIEDHLIEDNPATAKGSSWEKVRAQNQQTVEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH59</accession>
    <name>A0A1E3NH59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015796.1">
        <property type="nucleotide sequence ID" value="XM_019161874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH59"/>
    <dbReference type="STRING" id="763406.A0A1E3NH59"/>
    <dbReference type="GeneID" id="30178561"/>
    <dbReference type="OrthoDB" id="5370059at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="339" mass="37740" checksum="916F7C0EF3C046DD" modified="2017-01-18" version="1" fragment="single">MYKVLCCGSNGSGQLGVGHKRDLCELTECFRNNHRVLDIACGSNHTLLLLESGEAYACGSNISGLTSLQTLSENEEEMFMLIGQDEKTYKKIAAGWDFSILVDSSNKVHTQGDLRLCGGLGEAVQEVKTLTEVYHSADDDVVEIHTSLHSIIIIHSSGKVLAWGNNKKGQLFGDCKTLDMVYSPLSLSFDDLDYEDKENLRVVRCAMGRDYTFFHVLNTVSQSELIIMRSKNDRYKILQGLCNAIGDGHIEGNGRRSRWFRIKKGICLKRLKSMWSSVHVMYSTEEKSSVLKSFGNNVFGQLFPGLSTEVADFDVGTEHGVCLEKSEKKVCSWGWGEHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH86</accession>
    <name>A0A1E3NH86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_17960</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016604.1">
        <property type="nucleotide sequence ID" value="XM_019161826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH86"/>
    <dbReference type="STRING" id="763406.A0A1E3NH86"/>
    <dbReference type="GeneID" id="30178513"/>
    <dbReference type="OrthoDB" id="193716at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="106"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="360"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="610"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="684" mass="76581" checksum="0DAC10B4081090AE" modified="2017-01-18" version="1">MSRVMLMQSWNSTCSNILRRSLYRSPAVFQTHRHYATDIKDHIEEVENVSKEEESAKPEPPVTESDSSRIITSNVIEDTKLHPALKAAFIKSVGPTLTPVQEQCINEFLESKHGIVVRAKTGTGKTFAFGIPVIHSVLKLKDQNMKQADRYVNSVVFSPTRDLAYQTRQSLSDLWNASLAKSNHAPSKRGSYPKKSRLPNSEKNLIPLVIGQTPYKATMSHFVSKEVPPIVVATPGRFMDMLQNEPKFRTSFKHLQNIIIDEADELLNGNFKEDINKIIDELVSIREPIPGVEAESPDLKPKTMLFSATVNDDVFYLAERAISNDFPFVDVTGNKAQEVNENISQTLVQTESVFDTYVAAVQFVLSHAHEKNFKPIIFLSTTSSVDFISKLLMDVLRNENSRRRVLAFHGKLTQGKRDASQRIFREKDNAILVASGIGARGMDFPNVSHVIQIGVSSEIDSHTHKIGRTGRAGKQGDALLFTSKLEEPFVKALRKNGNKFTETIEFDGESPEAQEVAETIVGQTRSYLDLDDTFFKSITHYRNIPSKVANLNLTELSIDVAKTYMKLMGTDGVQSDGESDDDNSGKLTMSYNMAKNLGLDTRKIEDYFTLTGRRPNDSRSNGSRGGRGGYSNDRNSKRGSWSNNDRSSNDRSSNDRGGYNRNRQSGKSSRGGREGDADKRYSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHC1</accession>
    <name>A0A1E3NHC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73044</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016632.1">
        <property type="nucleotide sequence ID" value="XM_019164366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHC1"/>
    <dbReference type="STRING" id="763406.A0A1E3NHC1"/>
    <dbReference type="GeneID" id="30181053"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133367" description="Peptidase S8/S53 domain-containing protein" evidence="8">
        <location>
            <begin position="23"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="81"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="215"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="57289" checksum="CE1D51BA1BFFCF7B" modified="2017-01-18" version="1">MKLSSSLCFSILTSLAATRVSALVLPNLKDMPAVVSTADKVPVVAENVVADNAAAESFPSVELADEAILDVEFNENAIPNSFIVILKDDISDDAFEFHKLWISDVQVQSFAKLNKYEVDAFEQSVSSLNFQQQTGGIGHVFDSPGFRGYSGLFTPEVINLLRRSKDVAVIELDSTVHTVGAATENGAPWGLSRISHRETLGLGNYNKYLYDADAGEGVTAYVIDTGVNIEHEQFGGRAVWGATIPTGDSDTDGNGHGTHCAGTIGSEEYGVSKKAKIVAVKVLKSNGSGTMSDVVKGVEFAANAHVKEAKNPKSGFKGSTANMSLGGGKSPALDLAVNAAVKAGLHFAVAAGNDDADACNYSPAAAESAVTVGASTLSDTRAYFSNYGKCVDIFAPGLNVLSTYIGSNTATAILSGTSMASPHVCGLLTYFLSLAPSSDSMFSTAGITPKQLKQNLLKYGTKNALSSIPDDGTPNILIYNGAGGDISDFWNSEGSAESSAEDLERTVEIANDFAETQLDKTKEEIKQIINEITQVVDSVENVISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHN8</accession>
    <name>A0A1E3NHN8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015966.1">
        <property type="nucleotide sequence ID" value="XM_019164545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHN8"/>
    <dbReference type="GeneID" id="30181232"/>
    <dbReference type="OrthoDB" id="10592173at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="220" mass="24219" checksum="1881C0526EFA09D6" modified="2017-01-18" version="1">MGFLLPSELCPVPLLFGLCSSLNTLSTGIDTGKCLCIYLTKNSNLLQLIESVMSNSNVVKKALSNKKKKAVSTKTSKVPNPTYSPRKNNQFEDIISNEDESRLITGTSTVTPTEASGESSLLVHQGKHALGVDLEANYDRHYGSISNNTLEASNDSYCYMLQNLTSTQLYLLVAVLALLVILTALLYSIGDTDQLKEIWNKIFCWLGVMNCNTNNVRKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHV8</accession>
    <name>A0A1E3NHV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 17">Ketol-acid reductoisomerase, mitochondrial</fullName>
            <ecNumber evidence="5 17">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 17">Acetohydroxy-acid reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 17">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_34621</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17 18">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="5 17"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016830.1">
        <property type="nucleotide sequence ID" value="XM_019162897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHV8"/>
    <dbReference type="GeneID" id="30179584"/>
    <dbReference type="OrthoDB" id="10255643at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000003">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000005">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000013">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000167">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016207">
        <property type="entry name" value="KetolA_reductoisomerase_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000119">
        <property type="entry name" value="Ilv5_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 17 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 17" id="KW-0521">NADP</keyword>
    <keyword evidence="11 17 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="19">
        <location>
            <begin position="58"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="20">
        <location>
            <begin position="249"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ45717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="397" mass="44061" checksum="5B1F02DF57D060CA" modified="2017-01-18" version="1">MYRNAAARLARSSRSSTRALSSMAKRPTLVASAAAGANVARSNVGAVRGLKKINFGGVEELVHERADWPREKLLEYFKNDTMALIGYGSQGYGQGLNLRDNGLNVIIGVRENGASWKAAIEDGWVPGENLFPINEAIEKGTIVMNLLSDASQSSTWADIKPLLTEGKTLYFSHGFSPVFKELTKVDPPTNIDVILAAPKGSGRTVRSLFKEGRGINSSYAVWNDVTGKAEEKAIALAVAIGSGYIYQTTFEKEVYSDLYGERGCLMGGIHGMFLAQYDVLRENGHSPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPIFKENLKPVFQKLYASVRDGSETQRSLDFNSQVNYREELEKELVTIRNMEIWRVGKEVRKLRPENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHW2</accession>
    <name>A0A1E3NHW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_17002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016824.1">
        <property type="nucleotide sequence ID" value="XM_019161425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHW2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHW2"/>
    <dbReference type="GeneID" id="30178112"/>
    <dbReference type="OrthoDB" id="17687at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000015">
        <property type="entry name" value="GTPase activating protein (Gyp2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="252"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="634"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="940"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="872"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1003" mass="114515" checksum="54759B1073D01435" modified="2017-01-18" version="1">MDFFKNVATLAQNAIESINNPNHLTPDQLMQETYHLPATEHINDKISAEYIITSRVLYSFSGGTKHAGNYLPMPNSANVRAVGELILTENYLLLKKNDSAYDKTIFHLATIDKVQKIPNVELVQVRLSTHNGMILDVLSDTIRTKAEYFWHTLSKHHQAAKHQVPKLIEFQKSLYSEYLISKNCKNSKLGEKHIDITSHPKGGLGLQFKFPGDQSKEQEVVKLKRWFEYSRRYGRNLELVENQIFSSLILVGIPNRLRGEIWELSYGAMYLRFENVGEYEKILADNSGKKSFAIEEIEKDLNRSLPEYPAFQTKEGINKLRRVLTAYSWKNPEIGYCQAMNIVVAALLIFMTEEQAYWTLIVLCDKLIPGYYSKTMYGVLLDQKVFEALVKKTIPAIGDHFVKHDIQLSIVSLPWFLSFFLNTMPLVFAFRVIDAFFYNGPKTLFQVALAIIKVNSEKLLSCSDDGECIAVFKEYFIGLDEVQPSHEKLKRKFDILWEVAFNEFHVVDDKVLQKNRNAFKNEVFQGIDLFVKRAEIRNLPKTLHLTNDNLSNIYDRYYSKLLQDNSAPNRGNVSMDFEAFKIFMSEITVWVNLERNTRDQDSFLQRLYDNWSDESGEMHLESLVLGLNKLVDKDLMNALSNFFALYDTDKNGKISKETVLELAEDLIFITTPWREGLIFDSIANRELEDQIAERLVQRRQLLKQQGVQLNDDAEIAIPDEVKFDEEKWRTKQSVRYLSASSNFLQLAFKYAQPYEDPDAPLINLEDDDNDASLSNKKLDSITHNKALDPTHPVYITASMFRMVILANETYSSFFENQFWRSFELDKPGSMENGVFGGMVGNIRGMVNNVMADGARVATQVRKKMDEARSRGDTVNSIESGSSSFELNSDVSNFAKANGNNRTSMDSKGADSVMTGWTLESGALADNFDDANIEDLMEVPEEKRLLENNDDDGIIEQAKNKEETSTNSKQNEDTDDDEFGSFIPAAGDNVAIEKVANGIKDMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI24</accession>
    <name>A0A1E3NI24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_73249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016849.1">
        <property type="nucleotide sequence ID" value="XM_019164425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI24"/>
    <dbReference type="STRING" id="763406.A0A1E3NI24"/>
    <dbReference type="GeneID" id="30181112"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="597" mass="67676" checksum="04F19F68E9FC49F8" modified="2017-01-18" version="1">MNPVVAPISSEKLITVEEEKRSESVFSQFSRRRTEANGASLHKESKPTLDEDALIRQYLRESANGTIPSSINGMIGLKLSSEERTFYNAFVNGFIVSISNQLAHQKLLPGSIFVPTAMFDPLIQKLCMTCGASFLYRCSSGNDKELLSKVKESNSLIVKELVHRLDEMPIMETREWILIYFTLQNNRQKFVYEGRHSQTMNLISGVQAIKMWLETKKKDANTKVVEKIIEIENETVIGSDNIFYTNSLNQKADFQNKKRNDIPYWEYNHNGDTCSVDEFTEADSYILLDKLVDKTNLLKSANSNCNDGVILSSCEEPKSRTGHMVDMLEIEAKMLLSSFSNADKVKNSAEVTAFERTLLETFIFNYSGTLLIIDKSLVGCLTSPFELFDLIAPLLFVPIYKCAVPWMNNPTAGAALPMIELQAKVIWLSFKEKLDQKDLKIVHTIQKLAQYYTRPILPDEVWRSYPKNVSKKLAESCYVSEIVAKGVYLFSMKILDRSLSEGDDKVQEIVDSMFKILDRISMHSQTSVIAKWAFLVIGCAIVSEEHKVKLKKRLIGFVEALKTGSLRTSLIFLEAVWKDGVGLNALFIEKYLDLLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI70</accession>
    <name>A0A1E3NI70_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_22409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016942.1">
        <property type="nucleotide sequence ID" value="XM_019162259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI70"/>
    <dbReference type="STRING" id="763406.A0A1E3NI70"/>
    <dbReference type="GeneID" id="30178946"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="345" mass="39632" checksum="F8B8C40AB6B29293" modified="2017-01-18" version="1" fragment="single">PLEVTVDNIREGFEDRPYMPFRYPNNQTMSLVKLDVNFWSIVDRHYFDFMSQKKRIFNHYDPEKLKEHPFYKTLDTAEATAALVELRDFVVDHLTHRFPKLFTRRGNLVCNHLLDETYDLDVVAPLVVVTRMSMEDYYVTVLDPHTQERTCVGVSVAFGGGGFPIAPIVGQGMDAIHGPVPYYESKLKKSMNKWFERFTDPVERASIHIVWDNDLSCSELYSKQRELGHAAYAEYIQAVPFEAFSVRVERQTLIKLPKSNAIIFANHPIYLNIAHALADEPGVPAIVHKMMYDSPEGIIKYKHYELIRDHISPGLLAMVDAQVQRGLVAKDDPVRTIPGFPFASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI92</accession>
    <name>A0A1E3NI92_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016401.1">
        <property type="nucleotide sequence ID" value="XM_019164664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI92"/>
    <dbReference type="GeneID" id="30181351"/>
    <dbReference type="OrthoDB" id="3992895at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="293" mass="33039" checksum="C27DD53C1CAF731A" modified="2017-01-18" version="1">MSDYRHNITVPLGLAAKYWKAVEYGTLIVLFLLKTTNPIFKIALALHVGLQTMLILSFSPLVYYRSSIGDDESSSKGLIQKFTGFVPGLIKKLSGLVLQVTLKSLRAMSICDFKVDINRLASVVTYFLIIFRKLLALMVFTYVTSEDDESTHKKAFALFTIFMAMEMKYILDLSCIKNGGGTPKPLNGSKNLVYKTIKLLYTLEPPKISGSSKNELLASLHLVRSRLYHAGVGLAYILMYYTFKHATELIDDDFIWMSIMKSLIAIDALFIMSFVFELSGLNLHFSNKEDTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIA3</accession>
    <name>A0A1E3NIA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_13023</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a zinc transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016411.1">
        <property type="nucleotide sequence ID" value="XM_019159997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIA3"/>
    <dbReference type="STRING" id="763406.A0A1E3NIA3"/>
    <dbReference type="GeneID" id="30176684"/>
    <dbReference type="OrthoDB" id="78669at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904257">
        <property type="term" value="P:zinc ion import into Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045316">
        <property type="entry name" value="Msc2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133384" description="Zinc transporter" evidence="10">
        <location>
            <begin position="23"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="631"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="663"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="11">
        <location>
            <begin position="384"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="513"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="569"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="786" mass="88620" checksum="30FC6434EB62F255" modified="2017-01-18" version="1">MFVELAPVLLALPTLLVSQVLTVTVPPESPDSVTAYLLLNWVQSMFFASLSLVTMMAIFSYISVHERSKPGNGGESIASPSETSWFATVNNMLYLWTPLVFFFLSTHYLGLLRVISLFTVCQMLSTTSKKSSHRSFQMENNYSVSDCSFLLLFYKKFQVYIIFLLSFIFDLIYGLYFEKEKLLCRLFGYVLFITPFFALTTNALNDKISSSNLAFDSNIKIHLLIAAAALLKLVYSYMDVYNFKMMLVSIGSLIILLFSQFESTTTNKLKLQVLIKDIVSPDLLLQHYKLNYLTLNAVVLVITFLNESVNPDFSFAEAFAIFVGNYAVKFSFNILVMFLLNVENSLMVGTRNAVFDTTKADAETTLFGLFVQLINSDESKSIFNFLLLNVAFMFIQLLYSFRSKSLSLLSDSLHMLLDCTSLLLGLMASIISKNNLKTPSQKYPFGLNRIKTLSGFTNGSLLLGIVFGIYNESIQRFLNPVKLENTTELLIVSFLGFLVNLVGIFAFNHGHDHSHGHSHGHSHSHGHGHSHSPEISQVQSQDQSHSHSHGHDSSYKSCPSKDQSTTCTHSHDHDHDEQHNDKEDNHTHSHNTHETANEKKELEIKIPIEPLEDDDCSGSIDHHDDDNMHGIFLHIMADTLGSVGVIISTIVVKMTGWNFVDPLASMMIASLIFLSAIPLLKSSSANLLLSLPDDTEEELKYLLNEVLKIPGVKSYTTPRFWPQDGPKSNLIGYLHVQYFRTENSTHIKTNIDRLFSKSSAVKTFYLQLENEVDECWCRKDGVFSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIC7</accession>
    <name>A0A1E3NIC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5-oxoprolinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016949.1">
        <property type="nucleotide sequence ID" value="XM_019161464.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NIC7"/>
    <dbReference type="GeneID" id="30178151"/>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="2">
        <location>
            <begin position="241"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="3">
        <location>
            <begin position="740"/>
            <end position="1278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1316" mass="143994" checksum="C4CDBAC141664787" modified="2017-01-18" version="1">MTISDNSIKIAIDRGGTFTDVFYKYGTSERAFKLLSVDPSNYSDANIEGIRRVLEEVSGEVIPRGKRLDTSFISSIRLGTTVATNALLERKGAPCALVTTKGFKDLLHIGDQSRPDLFALNIVKSGVLFKSVVEADERVTMPAFAADPTGYDAQDLVDDKEYVRGETKEVFQILKPLDTEALITDLKQLKEDGIDSVAIVFIHGYNYQEHEKQAGKIAKDLGFKHISMSHEIFPVIKAVPRGQSTVVDAYLTPIIKDYIKSLLDGFEKGFEKHTRIEFIQSDGGLCSWEKFTGLKSLLSGPAGGVVGEAKTCYDADSKIPIIGFDMGGTSTDVSRYAGDFEFSFESTTAGIKIASPQLDINTVAAGGGSILSYKNGIFIVGPESASAHPGPACYRKGGPLTITDANLFTGRILPEFFPKIFGPNEDQPLDYEVTKKKFEELAELINKENPSMKKSPQEIALGFLDVANFQMSKPIRELTDAKGFDVSSHTLASFGGAGGQHALSIAKILKIKRVVIHKHSSILSAYGISLADIVTDLQEPCAAVYSQKSRDTLIMDCVRLKEKCLKNLVSQGVTVSSINYKIYLNMGYKGSDTRIMILEPKDGDFLKAFYEIHEREFAFNDKDKDILVSDIRVRATGNTSETWSERSAYKDYETTEKAFVQSGMEEKVTEIYFDEGLLPSKFYFLDKLKVGTMIPGPALILDNNQTLLVTPGSVATILPRHVVMDVDVGSSIAVTTNYVDPIQLAIFANRFMSIAEEMSRTLQKISVSANIKERMDYSCALFDNEGNLTANAPNVPVHLGSMSAAIRYQLERWKDDLQDGDILCTNSPIVGGTHLPDITIISPVFFEGKIQFVVAARAHHSEIGGTAPGSSSSYAREIYEEGVNIESWKIVSKGKFDYDGLKEHFVEVPRSHGVSGTRNIDDNLSDLKAQIASNQRGINLLKELFSEYGSETVLFYMRNIKKTAESAVREFLKTYAAENKGRFPLTAVDYMDDGSRIQVKIDIDEKSGSAVFDFTGTSLESYSNLNAPKSITYSTVIYVLRCLVNMDIPLNQGCLDPCTVIIPDNSLINPSKFAAVCAGNGMTSQRITDCLFKAFGLTSATGGCMNGLNFGTGGLDENGNMVKGYGYTETIGQGNCAGIIVKDGAKIGFNGFSGTQTNMTNTKITDPEIFEQRYPVMLIQYAIRKNSGGKGKWRGGDGLVREICFTSPVHVSLVTQRRVFSPWGVYGGEDGKRGINKLGRNRGNGIIQWIQLPSLAEISMRKGDILWVATPGGGGFGKETDSDEYWGVATTMKKFIHIPISSGSIGAMQEAANTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIG5</accession>
    <name>A0A1E3NIG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_17139</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the PAM complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PAM17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016989.1">
        <property type="nucleotide sequence ID" value="XM_019161478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIG5"/>
    <dbReference type="STRING" id="763406.A0A1E3NIG5"/>
    <dbReference type="GeneID" id="30178165"/>
    <dbReference type="OrthoDB" id="5970083at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013875">
        <property type="entry name" value="Pam17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021:SF1">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08566">
        <property type="entry name" value="Pam17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="201" mass="22920" checksum="0506781B8599A4EB" modified="2017-01-18" version="1">MLAMRNIGIRVMKPSFLLQRQSVLRPFQKRLNSTAKKLYEPSPQEAQLASKMSWEEFLALRKQQRRIGALGSVAAALLTMGAAYSYLSQVEIDPTETLFGLDVFTVYIGAIMCTGFVGFMFGPALVGDPLFALINRKIMATYRTKQTLFLKHIAKMRVDASRQSMNNPVPDYYGEKIGSLAGYRQWLRDCNEYRRKAREFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ07</accession>
    <name>A0A1E3NJ07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-type lectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17792</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016446.1">
        <property type="nucleotide sequence ID" value="XM_019161744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ07"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ07"/>
    <dbReference type="GeneID" id="30178431"/>
    <dbReference type="OrthoDB" id="10265193at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133394" description="L-type lectin-like domain-containing protein" evidence="4">
        <location>
            <begin position="27"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="5">
        <location>
            <begin position="26"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="483" mass="53169" checksum="32038DE530482E98" modified="2017-01-18" version="1">MAMVGSISVLSLSLVLLIQFFTASSAAVGELAEFSLPNLVSISSIEELSSRWIRSGDTEYDEGRIVLTPKPKHIVSKDETGVQLGSIWSAHSPASQLDAFTIELTIRSLGSFGLTNAGLSLFLVDKGSASLGQSNFGGPVVYKGLQLLVNMDEKLGPVLRVYLNDGKSINLATDYLGAYRYEFQGSHVPLTMKVAYENKFLKVTCDNKLLFQTDKVNLSSLLSGDLKLGIAASSPKNLKFHEQFEILKLTSYDSATADMKDDNDETLVAKHADSLDSRDGTGAAKFLQQQQKLREKLATSKSGVDPGSSFSSPSSESSSTNEELEYVKTRLESIINLVQKNGQARLLEEFSQLSNTIEKMSQSLALLQDQFGTLNNKYNELSNMFKKQSDLLDNYDTTLRSFDKVMQNQLKSSDSLDSKLSTLSSYYSSSMRDNSTPDYSGADSISKLKSLIYMIFLPLLALVALVALWIHRLRNDIKHSKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJG5</accession>
    <name>A0A1E3NJG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017363.1">
        <property type="nucleotide sequence ID" value="XM_019161112.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NJG5"/>
    <dbReference type="GeneID" id="30177799"/>
    <dbReference type="OrthoDB" id="1893551at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1204"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1249"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1288"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1480"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1204"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1256"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1308"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1480"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1500"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1516"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1541"/>
            <end position="1550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1550" mass="172015" checksum="B9B76EA9C0E51B0F" modified="2017-01-18" version="1">MGDELTFSLDDLQKRDILGRSLLHFLAISNDVPSLVKVLKLITKTNNHHCLLAEDVENHWNVLHFAIDSFNFAFVKTILNFSTPVLGKLLKHKDKNNLTPLDLLQTFKTTFDASSSLVYSIDPEGNYKSRPRMDPSSAASHSSLSSSLILSLPSGEFSEISLTDNLFPPPSPNPLLPSIVSKFMSSSTHSALITTAGYLYISNPQPTLFNSSFCRIKFFDNLYENGEKVTDVALTSGHTVVLTSLNRVFAFGNNVKRLNLYPASSSSSNYHYSQDLHSLIPISISVKNRSSSISPPTSGTLPSGNNFSALGALGSFANLDSSAADAAFSFRGVAASNNHTVVYSKLSLHIHGLNLGQFGPLTNSIQTSNKPAEKNQGFLSTALSAVASSAHSPWKYEDDPIHQVVALDLATLVITQSSLIHVYVAGLHVKISLPFNKDLKDSWNKFKPRVLSMPRKIVKMLAPLSPDHFSGEKAVNNSAGNLHSTYSVLTLLNSGEVYVFTFPRYSSSKDSFKESVKFTLIWNPARTEMRATDVSVGALETNNTTGIGAVLCTQSGEAFKRTKTKWVRINDVSKIKNVAVGYGFPIIGSFSTDDNQFKTILLRQEDKMLKHEILPSKIIVDFAKLSPLQGHLGFNESDVPKQSAGCDVDFFSSCEYGDTDTYGDYDHDFSDKQIEDIKLGIKFEKHINPLTYKHRKISHVPFLDSKASVDISMGIHDFIDESMPHVATKFSNFYDYEVAVKNPAGDTQLNLFVHKKFLFNRLGFKHSTYSLERKDLKFDFGESSATIIGDMDVRNVALFLHMLYTDVAYDIWNNSDHASASLKTGWDRLLYSFPTVNLVKAIRATFTDDSTGDITVKLLDGDVTTNRYLLSCRCDYFKQFFTEFWSQSNVLDFSHVSKYTWSLVMKYLRGDSSDEIFYNTIKELATCCSENISQRCTNKKKSNRLLPKEEVKETGLQSSDDFVNIVLEVLYFSNELLLPNLRELCELAIKDCMNFDNYDIFLQHAFISGSKQLFANCGWFIFSNLTLCYKDQRLNANVIGEDCACALEAKIHELVEIYIPDRKKLKPKSPSRSKQSLRRKKLSIVNENEYHNTASFIRNTEQFNEYYLHPYLWDEHGISEDQIILRTPMNRRKSQTPIFKNLRSSSTFLSSPVDAADFKQEGSLPHIATGLASRSSSNESAISDDFNDSSEGFIVVQKTRRKSSSIPTLTNSGRSYSSSLTRPPFDVNTMRRESSGNILTSKSVNEPWKILKPGTSSADKSGSAGSDMGINSVGSSTTTLDEILTKSKSNTKLDTRPKSKITAPLRISQKERKLREREESEKAAKVDGNKGEKDPKSGKAPWASSSVWGMNAPRVVASSDDPVAVELPSGIVHAELNTATLLASNKMNTVHFPSLEELRSVKVNGKVANDSVFPVIGSNSSTTLLNDPGHYDGFNGGMSASSSMNSSAALNIAKTKSLEEIREEEEFAKWWKEESERVQRALKHPEDTNTRIGGGRRDSNSNNNGNSSRGNRGNRGNRGGRGRGGVKNGGRGGRGKSKVGNRYDDVQVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJG9</accession>
    <name>A0A1E3NJG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_72373</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017405.1">
        <property type="nucleotide sequence ID" value="XM_019164180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJG9"/>
    <dbReference type="STRING" id="763406.A0A1E3NJG9"/>
    <dbReference type="GeneID" id="30180867"/>
    <dbReference type="OrthoDB" id="167718at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12400">
        <property type="entry name" value="RRM_Nop6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034228">
        <property type="entry name" value="Nop6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF51">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="92"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="165"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="236" mass="26409" checksum="DA727B4139E53BCD" modified="2017-01-18" version="1">MSDTETAQSAQKLSKKQLKALRFKAKTSNNAAEELKTVEETKAKEIENAKKRKVEEEEAAAAAAAAPKKKRKTRRGKKHRGEREGDNSGPRFILFVGNLPYKVTEEDINTHFKNCKPSSIRLRTDKGCAFVEYKNADLKESKRQMDIALRLHKSTLGGRKINVELTAGGGGNSQDRKEKIKVKNEKLEDERTKRTVAEREKKRKADAAKEKEAGGESEPAGEDGVHPDRAKLLQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJH5</accession>
    <name>A0A1E3NJH5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Actin-related protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Actin-like protein ARP4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_72313</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Chromatin interaction component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Is required for NuA4 complex integrity. Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NuA4 histone acetyltransferase complex, of the INO80 chromatin remodeling complex, and of the SWR1 chromatin remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the actin family. ARP4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017339.1">
        <property type="nucleotide sequence ID" value="XM_019164157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJH5"/>
    <dbReference type="STRING" id="763406.A0A1E3NJH5"/>
    <dbReference type="GeneID" id="30180844"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000203">
        <property type="entry name" value="Actin-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="350"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ46226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="502" mass="56619" checksum="DF9C54D2920259B3" modified="2017-01-18" version="1">MSDKTAAPQVYGADEINAVVLDPGSYTTQAGYAGYDQPSIVTPSYYGIHTNSESGETTKVFDENLLFSRPKDGAEIRSLVSDNLVIDWDGAMDQYRYLFQQLEINPREQPLLLTESTMNSYKNKIKAMEFFLEEEQFCAFYSVKQPTCVSFAHGRPNCLVVDIGHDLTTATPVIDGLCLRNQVRGTKYAGAYLNQQLRQFLKSKAVDYSAPFMVKKKEGVYWENNQTKPIFETISNIDNIHPSVRDFSYLRTLREMKELLLECCTDDELTLPADAEKELDSERTRWFELPSGLNVPFTAYERHKISNSVFNPSDTLCDVVENWENPYDGNIINTIGTKNDLTSKEYVPLRRAKKTEDEENNNGNAGKDENSQNPSNDSNTKDNGAILKHEKNDSSVGVTDLIQNVLENLDIDLKPQLANNIVLTGSTTLIPRLNERLNNELSLLNPSLKIRVHSAGNTTERKYASWIGGSILSSLGTFHQLWVSKAEYDDVGPDQLIVNRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJR2</accession>
    <name>A0A1E3NJR2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_142195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016696.1">
        <property type="nucleotide sequence ID" value="XM_019160278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJR2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJR2"/>
    <dbReference type="GeneID" id="30176965"/>
    <dbReference type="OrthoDB" id="3997968at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="523" mass="60118" checksum="16CB0F8FFC9179E9" modified="2017-01-18" version="1">MSNRHPSTPPPMESESNPFFTTPQVKSTSKNTSTFEPTPKSSHTRKNSLTNYGSYKNKSKSSNPILFTPQTPYLNNNDDDYPSSLLPLSPQTTTKKNHPVIDIFSKQHHQSNFLNNQRNLTINPLRTPEATPRHLSRKRDVTAFMDKDTSKLGHPLFQTAPLTLGSGRRLSESMRRNDRAAIEPEQVQSNKKGLLEFGSSIKVQIEQDEEEEDDEKVREEEMEDDLQEKRSVQIRDKLLNTEYEDDREKMLMNYTSSESESDEDNKEKTDLLRRRLNIAGAGMSEESLSNAKGPITPPMQIMDESFILEKFGNEKCKFEDIEDPEDVQKDLKNRVKYSSPFLVSAGKIEKAQKKKSIFNPRFENEIEMVYHATGEKFYVGLSEEGKKIKPKKLNFEEFRGLGIGSSEDLLRTPPMSSSHKMSIKGLLNHDEDECIPSEDEDNRAAEEEGVRHEKIENPFRTKFATEKDNHRTRYTVDQRSDGTGNKEMHNIEYVNQTTGRHIVEEMDDEQLRIKPKRLDFSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJW6</accession>
    <name>A0A1E3NJW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Telomere-length maintenance and DNA damage repair domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_146575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016981.1">
        <property type="nucleotide sequence ID" value="XM_019160474.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NJW6"/>
    <dbReference type="GeneID" id="30177161"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere-length maintenance and DNA damage repair" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2193" mass="253414" checksum="3CF53796722590CA" modified="2017-01-18" version="1">MLRGSIDSICHSLSSSKIRERNEALEKLQDINFNSTSTKNAFDRDLLLKLVESLFFYIDTERTIFEKSNSNSIVEVRLNKVSSILRSVIVHLWATKKFISLYKPRQSEKIIELLLKNVMYTPVDSEIQIFDPTALNCVKAMNHLIRIQCFRDHLYLTSSESILSVSIRSLNLLVTDTINSSKNENLLVELFTLVHEFLNPSYSSNTILFQTTQSTTKGSYYSKFYHILLNYFKNIFEDSKRERESLIFVFKIVNKCIIDMSSANINLCHSFYKLGVKFILEVKTIVSKKLVFELAIFFNLIGPYILPMEVLKDNGSNHNINTTNVHITPSPELSLLPASKSEKLELTETASNIINPSSEADESLPFSDLEGSISRDLSTSAPMKKRKRMNSADSEKSSLLQEISDLSKIIEIGFNLFSDISLHKQLELESNSVKIHIFPDTTRSASNLFSLRYMYLSKGSHSTPWLLRLGIVHLLICFYELKAKLSTLDTETEFITASKRLRISGMGLGLTFQISDYIRSFDNPLDCLLSLLIDDPFKNNVLTVTISQLLAFCFEYLSSNRKLITVEKLSCSVLLNKEEILEELMIVFEKPDNQLKFWVLFAINIFYSVMATFRDEESNTHVHNLSKAGTSSFNKLIKYCLELLKDPYICRLACIFLTHFSMYNFLTKSNFSHLEKTIIQQYENVIDLSEINGPAILCYESILFWTTAVNICKNFKFKNIKLRNSLNAYDSQLFGHKVSHWFTGKIDQLSTLNNAYDIFSITMFVGWLSGFGSMKFAKRDWQNMERVYDGHSYRLSQEISEKAKLTQYILKSRSINSRMHVNNMDLYISEGTLSNNDVTKSRVRLMFINFIETSMDHHAIEWCLAMKLFLDVPCTDYSNLVEKTIDSLREHFHSMKNDKSLQLFLHKLLDAFSSISSESKDWISKTSHIIALPMVLEASLREINAVDKSYKDLEFELFDSFMPSASEEVLDESFPVYSVYNFASLSHCLTLEQKATDYCLKFSSVNDNVEQALETALKFTSKIKSKFQYIASQIEILKFIETNNVDTISEQVIDEMFSSLTSLLEDHLTKTNESSLILIARAFKAFCKRWMNPQLPGIDSDGKAVYEYLTNLYQKNMIYTESVLIAYLDLSMQILTCFNNSSIEFNNKEIWSTSIEIFAMLSNFNKCQVGEYIKTFIQDANGNKLEIYSSFVKSFKDPHFSIESSATFCFFMTRISAASESLTVATVCNLLELSNHSQILMYLPYAIRSIITTNNIPDVHTLFWNFKEIFFKCWWSFEFSIEEFPFKLFEFSTLDDFFLATYKEMTALSLAYNHKSEISRISKITRMQESSVVNDSMALSITLSWTRGGIRNKIFKSFEKYFTSSKTLKANIKEQFLLIVFQLFRFCDCSSENELKNTFAFETNEKHKEDLFSKNSQKISFLDEYELYIKPKSCVDMINYFSDTCGIDETWTVPTVYHLTSKILLLVDSSILELEKKIHLRRLKLLYMLASKGFANSNVCEIITRSLVPYLKDDNLMDDAASMLNCVLCENSKSLSNISIEVWVLLLCSLFDGHRSSQLDNLRITLSRFQSDINMGKFSEILDFGLNTIHKKNTTPVPNLIEFINVSCEDAKNTHILIKFLSLLFERSVQLHTLNYEKLHTSLNEQFVQNIYKIKIDYASELSTPMLIWVGKCLGIYYQFYGNIPESRILEYDSYMMQQNNRLEFNKLVKSLDMIFELMIKDLPTSNPRTRYCFETIVGVILYKKNVLHEDITSYISYDSLFEPFEDFIYPMGNYVCSLLIEQVENNNLTYYRHGLEQALNGFSTNIKAFKLEKWLTQILFSIINELTSHTSIIILLANYICHIASFSVKCFCPLILYFIENQPIKRGNLIGKMIHDFFAEDINQLPKEAIQLFVELVLLIRIGTKNGNDKFVSIFRHFDIPPIYEAALYIKKYKAALMLFEDYYCNVDLITQEEVLNDPSYNMSLKEAYEGIEDKDLVFGLPIVPKLDYGIDILKNNNLKWGEMMFSNAKFESSLLWGNFDKGNYLNSITSGMMELGWSGVSNIMSEYSYETPNNEKDSYSDMFYEQLWKLNQWDISASTEFVSENKCIYSILKQIKEFPFDAKSICEKSIEKLVKTEVKLFTKPGATSVESLESWVRTLSLCSIFEKMTSTNEGKFEDSLISFHESTSWFKNATVTEFENILLGRRSLFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJY4</accession>
    <name>A0A1E3NJY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Impact N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_58758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017560.1">
        <property type="nucleotide sequence ID" value="XM_019163317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJY4"/>
    <dbReference type="STRING" id="763406.A0A1E3NJY4"/>
    <dbReference type="GeneID" id="30180004"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF17">
        <property type="entry name" value="IMPACT FAMILY MEMBER YDL177C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="175" mass="19103" checksum="5CE12B4C47DF0D69" modified="2017-01-18" version="1">MNPKWSVSNVLVQKKSKFRAFCTPIKSNLSIPELLNDLTTLDKTIQKASHPTMYAWKTASSELETAGCNTAKSKKSKGKKTDELTVESSVLSKLVNLNQGFHDNNEGGSGLRLLGMLDRLHLVNILVVVSRWYGGIALGPARFKCMSDVGMEALQNGGYLASKPGKGWTDCSKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK26</accession>
    <name>A0A1E3NK26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_131043</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017618.1">
        <property type="nucleotide sequence ID" value="XM_019160017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK26"/>
    <dbReference type="STRING" id="763406.A0A1E3NK26"/>
    <dbReference type="GeneID" id="30176704"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010590">
        <property type="term" value="P:regulation of septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000780">
        <property type="entry name" value="Rho GTPase Rho3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="24816" checksum="B2220BE404FFB65E" modified="2017-01-18" version="1">MPFCGSPPKPVQRKIVILGDGACGKTSLLNVFTRGYFPKVYEPTVFENYIHDIFIDGQQIQLSLWDTAGQEEFDKLRSLSYSDTHCIILCFSIDSPDSLENVKNKWVGEILEHCEGVKLILVALKADLRQSENANTNNGDDGADNLGGTSAYNNDGQLKKLVTYEEGVSVAKQIGALRYLECSAMKNRGVKEVFTEAARIALTARPGAASANGQPVADNSGSSSSCAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKA2</accession>
    <name>A0A1E3NKA2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_132071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017698.1">
        <property type="nucleotide sequence ID" value="XM_019160027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKA2"/>
    <dbReference type="GeneID" id="30176714"/>
    <dbReference type="OrthoDB" id="3989460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="175" mass="19736" checksum="28E659FE5CE5197B" modified="2017-01-18" version="1">MLTQSTTNMTDEDGPDVHPIELTVQALLETPLETLNLNLKSLYESQMILHTILYKMERTLIDTANNLRPGSFPLQEQVVPGVRGDRNGNNNSNGHGPNSCCDSSKGTANCSSVNGKDDIGEEGIRIDHSDLDGELDLKMYLERMERLRRKLRRIEKIIDEIEARVGRIENSMSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKL1</accession>
    <name>A0A1E3NKL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Restriction of telomere capping protein 4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017739.1">
        <property type="nucleotide sequence ID" value="XM_019159813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKL1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKL1"/>
    <dbReference type="GeneID" id="30176500"/>
    <dbReference type="OrthoDB" id="128308at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028094">
        <property type="entry name" value="RTC4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14474">
        <property type="entry name" value="RTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01312">
        <property type="entry name" value="RTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Restriction of telomere capping protein 4 C-terminal" evidence="2">
        <location>
            <begin position="277"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="469" mass="53786" checksum="0A17C6827B64327B" modified="2017-01-18" version="1">MDRKPQFPFNKSLNSLQSSPRRYNSAGPDDDLVLTKPPQRETAIMPPSNQVPKSTHTVPSSIDSLMPVTSRLNRPTKRRSNAIYGRQIKNDLSPKKQKVTATERLSAKKKLDVYVYDLGEKESDLLHLGEDNDEMESIMKEFDVDEMEQKSHDLQDAVETYKREREREKEKERGKAREKGTSVSRHISEQLKNDTLLGMKTRDKTSKAIDSFHKKNSQQGHRLRDLSLQSLINNKDKSDAVKRKYAGKKKYIKQDLIQKVEHIREIVEKYRAVVIDILEGQIGSYFYNEAKKLQQASPLMTIRGEDISNLPKERFYGYIGAVRGFHIGKAIERDPTLRNLIKEKAKFSKVINFWGVDNFTQYVLAPEVIAHIVLEQGKLEDLDAAYDEMEETNDYGIFVTNTVDIEGKDSIDTPLDEENIEFIVSEEEDGHNTGPLGADQYIHDLESSRDKEDSDTEENSKDLIDDIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKN3</accession>
    <name>A0A1E3NKN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16552</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017828.1">
        <property type="nucleotide sequence ID" value="XM_019161253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKN3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKN3"/>
    <dbReference type="GeneID" id="30177940"/>
    <dbReference type="OrthoDB" id="27962at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058567">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058568">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26280">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26283">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="3">
        <location>
            <begin position="5"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="3">
        <location>
            <begin position="201"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="4">
        <location>
            <begin position="340"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="5">
        <location>
            <begin position="577"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="6">
        <location>
            <begin position="854"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 fourth Ig-like" evidence="7">
        <location>
            <begin position="1068"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1181" mass="135110" checksum="60DA7F2ABBFA975F" modified="2017-01-18" version="1" fragment="single">MDSVTFVSPATVRALLVPVDELSQSDFEKILETLKRVKDVRAVDLIHNPGKFNPQAYPQGHLYYNFITRDDDQESLFLHDFEPFRKTAIVIGILKWHADLTDDRIRSLKSTLKKAYPAPISQFLMIFDSPKTYSSKLSEVYTVDHDTANMETEICDITSRFLSNFSTYASAYEHTTLRSPGNLDGSIGRAKKKIASSFELNSEKVKQMSAKGRKLKLSANFYLMAGNLKSALSDLCEAIFNLKLANDLLWLASALDALAVCIFLLSSIGAPYQLPSFIESLLLKAKDIDSIFLSPNSSPRASLQLNGLGHDSSSTLNLPVVPLETVEKTIFNCGRLSSAFYEQAKLAHTDYVPQIAVSESLLRYASLLVSIDAKGSFDLDLIHEILFSSSTNTNRKTSKNFDLEHFNSLCSHVLDSGFRHLSLTQQSQIFFTLISLYSKTNMQMKKCLMIKNFLDRIIESKHVISINRFEYKELNAMLNQYCENYGIKVKNSAYVGFTNHLQKKLLFQLLSFCTKIKHYEGYTYYGSILLKHFRQILTEKEQMDVHQKIGEFCSLTGATPDYWDQNLFLDFEFDTAADNIVEGEETGAHIILRNAFAFEVEVRELSLYTENNFPLKLVTMDTKKYTEPITEFPSIFLKPLSEISISLAIIPEQHGKVAIKGIIASIDICKKQTFVSKTHIPKSFPPKLNKLSDKNQTDLVRTWEISVVYKQPLLKLMDVKLSDKWLMLLDGERKQFKVVLKNASNTEINHLISKFKDSTTDRLNAELNNKALQPNEVYEIEYQLLMMKPFKILNKNELAQIKGNESFHLEVEIRGKLGVNEANLVLEYSHQKDTASEFKRSLTIPVNLTVYPSVELAGCDIISLTSNTKISEWNEDPCWEYLRQMKEQNYKLSHFCLLALDFVNMWSEEMEICIECNPQNDKEGEINGSMNSVGKTVEGKFSVTSQLQSRKNIRMFIPLLRMDFDEEYLNQRIPSLRNKQFVVDKKTPQAEQEFIKHAFWYKEEILKRVSATWKVSSTSSSSSSIHAGKTGTIDLRSFRFSSKMIEVLEVEKIGILLTLLDQENQPVDLNNANLNRFYTVRLQLTNRNKHLIFGIIRHIPVCKDPPYTFEKKILINGVLQYSVETPLKPGESRTYDLGIVFLEKGECEWGALFDEVEGWEEGDINIKKQHLQREQLKVKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKZ6</accession>
    <name>A0A1E3NKZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_32937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017912.1">
        <property type="nucleotide sequence ID" value="XM_019162793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKZ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NKZ6"/>
    <dbReference type="GeneID" id="30179480"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="561" mass="61445" checksum="0E0BEAD7FF450DCC" modified="2017-01-18" version="1">MPMPDMSNAIVAQDELGRPFIIVRDQSSKTKSSGLEALRSHIQAARQVSDILRTSLGPRGLDKILVSPDGDINITNDGATILTEMQIDNQIAKLLVELSKSQDDEIGDGTTGVVILAGALLEQALKLIEMGIHPIKVANGYEKASKIISAHLDEIADEIFIDKEDLDSNLLASSDLFKAAKTSLGSKIVNKYHNQFAKMAVESVLKVADLDRDDVDFELIKLQGKLGGSISDSKTINGVCIDKDFSHPQMPKFINNAKIAILSCPFEPPKPKTKHKLEISNVEEFKSLQKYESDKFVQMIDSIKKSGANLVICQWGFDDEANSLLLANNLPAVRWVGGSDIESIAIATKGSIVSRFEDLSSEKLGHADKVYEVEFGTTRDKMIVVENEKSSTAAIGKTQVNTVTDLIRGSNKMIVDESIRSLHDSLCVVRNLIKNNKIVYGGGAVETSCSLKIMEEADKQKGIDQYAFRAFSQALDVIPMSLAENSGYNPIEELTKLKAAQVSTNSPHLGIDCLGSNNNDMKEMFVIETFIGKKQQLLLATQLTRMILKINNVIVSGKDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL41</accession>
    <name>A0A1E3NL41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16663</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017962.1">
        <property type="nucleotide sequence ID" value="XM_019161297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL41"/>
    <dbReference type="GeneID" id="30177984"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="421"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="112"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="522" mass="57991" checksum="7B40765CE4ACA326" modified="2017-01-18" version="1">MDPSSVPRRSGETRPESFSFVQGGSLNGHLPQAHIRADARLQEQEFTSYKALDYQKGGIEMHELTVKKVRTYAENDLDAGHRHRTTSLEEVVVEAEEENETEEDSYYPKGWKPMLVVLGSFLSCFTMFGIFNSVGAIEAFVQDHQLASASVSSVSWVFSIYMFVSLFLGLVVGPLYDTFGATYLLLTGSILTFVGLFACGSATTIYQFILSFGLCNGIGAGFIMFPGISVISSWFNKEKRSFYIGVAQTGGSIAGIFFPILLRYLFDKYGFKWAMRILALFNLGVTLVSLPFTRDRLTELRELRGEPADERPFWMKLRGSIDLNYFKDKKFMVLTSALFMNEFSLLIVLTYLASYAMAYGASASESYVMLTILNVAGTFGKFIPSYFAQKYGCFNMMILMSVSMTLECFIIWLPFGKHKAALYVFIVLFGFAYAATYALTGALVGAITAKTKDFGKRYGSAYAIVSFGNLISLPISGSFVVNKTTSDYNHMVAFASASCFLASFLFIVSRWTVVGSKLRAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL49</accession>
    <name>A0A1E3NL49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017972.1">
        <property type="nucleotide sequence ID" value="XM_019164321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL49"/>
    <dbReference type="GeneID" id="30181008"/>
    <dbReference type="OrthoDB" id="3998245at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="620"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="726" mass="83119" checksum="367B4C9E1F70A276" modified="2017-01-18" version="1">MYNALFVESVMICTCTNKFDNSLLNCVHVSQLEEFDVLEIPKRVKISYFLAPQFFHAVLKYLMAVNEDDLSETSNFNKEIEASKKSSLEKFLDIISIEFNKHSKVEDEYDDNPDNELHDSSDSSEKNESNGKGIHSKNMSATDSTIFQVEILNLFLTQIDIEILTAWKSDDYSSGEEKSFGHQSTRKKLMEEANKLIWGANKDSSYDDEVNKTLRNDATLYKKKVLHRNDYLMQKKVSSELYNLGITDVIDLINQLYLYMSVILCIGILGKRAAWGITQMNYLDNQSKLILKAWCKNEAKLKEFDLQVDEKEDLADVEIKGNIASTGSLNLQSSNLNYILGNAGWMNVSDSNDVELNCNAKILKKCEEFIARNELVESQNIRLLAANEELKIKIQSLYEAIENLTKESSNELQVKQLSQHTEEELSKADVSSQEVTQDQMRNKFRRENEQELMEMEEKYHGIIASLKGKLTESQNKNYDLKEDVKYLELKNVDIKQDKDLFIPKLSAEKQYFENLHYISCLEVGNEKKDKGGDGNKKAEKLEKDDKSSSGDVIMLLTKTLIKKQSEYQETVDGMKNEILLFSSKYEILKSFTKKIRERNLQYSLAVNEMLENDRKVWEWVMGLKLRIKKVEAQIGKMRDKVNEALTMAGVSPDMAREVEGLRRSINYGLNDMEGYLWSGKQRSRTSGGPEEIGRVGTAENGVAAGNEVGPEDIHVRCESEETRIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLG6</accession>
    <name>A0A1E3NLG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PXA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_73000</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018098.1">
        <property type="nucleotide sequence ID" value="XM_019164357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLG6"/>
    <dbReference type="STRING" id="763406.A0A1E3NLG6"/>
    <dbReference type="GeneID" id="30181044"/>
    <dbReference type="OrthoDB" id="5582218at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="3">
        <location>
            <begin position="85"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="492" mass="57899" checksum="1175CB60101E83E8" modified="2017-01-18" version="1">MDHSGSKSTFLQKSIKQRKLGLLKQQQQSSQQPSAQLSSNPRKKTEFNDDIEYLQQLTQELLFGTIETSETKRFTERNLPPLTSSNDLDVRLYALFALLLHNFVYEWYTKSLKLGSRDEFTKELVFLFAHISRNLQERINSADDEFIRLIVTDIPYLLEKHFESIQEVECMLARDGYEDTNEFSTNEIFVNEWMMRFSDGLVDDENIATYRRLLVKSVVCLILPHENIESSISREFVTSLLDGIVLKNVVESFCDSFVIWDIIGKICVKMSSQPKKEIEISATRRKSSIHRILEFFVIDEKFEVRTENMLEFADFIPLFSFVNFLTLFDFRFPILFTFLQVVLQLMMKVGYFKRFISSSIKRVVCEKALKFKNAEKLIDLLRHMMFPEDDRCQMKPRYIPQNENELKAIYDFNFAEVVKFLSSDSNLSRIFISKYEAQNPTAVKAKAKDTMQMFKHRQINQILIQKLIDLTIARIFPELQVRDTVSMFRIHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLL8</accession>
    <name>A0A1E3NLL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cystathionine gamma-synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_105364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018137.1">
        <property type="nucleotide sequence ID" value="XM_019159683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLL8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLL8"/>
    <dbReference type="GeneID" id="30176370"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000072">
        <property type="entry name" value="Putative cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF35">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE (AFU_ORTHOLOGUE AFUA_7G01590)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="41500" checksum="D5B02CA5A4F639C5" modified="2017-01-18" version="1">MPGISTNSIHADQELNRVIDVVPPINVTTTFRYPDDPEDIKPAVEYRDAEYDPSTSRPIYSRIGHPNSTRVEKVLGKITGGECVIYNSGLSAFFAAIMHFNPKTVAIGDGYHGCHNILHLLEKNYGLKIVGLDQLDQLSKGDLIHIETPENPFSIVHDIQYYVTKAKEIGAFTLVDATFAPPPLSDPFDFDVDMVMHSATKYFGGHSDLLAGTLTTKDPNLKLQLASVRPFLGTNIANLEAYLMLRSLRTYELRINQQSTSATKIVKYLNEHKDSYPSLTKIHHSSLQKDAFVAKQMKGGDAPVFSIELSTPLKAKSLPSKLKYFQHATSLGGVESLIEWRAVTDASASQNLLRVSVGIENTEDLIADLDAALKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLN1</accession>
    <name>A0A1E3NLN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peroxisomal membrane protein PMP47B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_15032</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018143.1">
        <property type="nucleotide sequence ID" value="XM_019160637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLN1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLN1"/>
    <dbReference type="GeneID" id="30177324"/>
    <dbReference type="OrthoDB" id="2019556at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080122">
        <property type="term" value="F:AMP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005347">
        <property type="term" value="F:ATP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015230">
        <property type="term" value="F:FAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044610">
        <property type="term" value="F:FMN transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF5">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="162"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="278"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="33"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ47030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="41863" checksum="9EA25F4645965AE7" modified="2017-01-18" version="1">MSDRSISHAISGGLSGLVSMAVTYPLVTLSTNAQAKTRNQEEETTEEEETAKIDTNDDEDDATSTKSSVSKLPLRTVHSEIPKGTDEIAQSTLSIARAKLLQLLRKLLHRLKFMIQNSKKYYSGLESALLGIVAVNFIYYYVYNLAGNYFKKLNKAPQSAGLNVKESLLTGLAAGIVSRVLTNPLWVANTRMTVKQKMDVQVDGNEGGEAKKVDVSQQNTFRVMYEIFEKEGFNGLFSGLGPALILVSSPMIQFTVYEQLKNLLTKLRQNGRAGYTNISSVEALVLGSFGKLVAILATYPYYTIRSRMHLSTSNENSFKVLYGVVQNEGISALYGGLNAKLLQSVASAGLIFYFKEEMMTLVGSAIQKLSVLSKESKVLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLX9</accession>
    <name>A0A1E3NLX9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_57708</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018265.1">
        <property type="nucleotide sequence ID" value="XM_019163252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLX9"/>
    <dbReference type="STRING" id="763406.A0A1E3NLX9"/>
    <dbReference type="GeneID" id="30179939"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF30">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04702)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="484"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="60240" checksum="228D50DCA907BC4F" modified="2017-01-18" version="1">MEKTEDGKDKVNLIPTPSDDPNDPLNWTFKRKMLQMFCIVLYCYGGSVPGCCIYSILPEIAADPNVNITVADLNAGTGYSFLFLGLGNLVLLPLAQQYGKRPVYLLGLIGVIAFNIWQPYIHTNGEWIASKIINGFFYCTIESLPEITVTDMFFEHERATYMGIYGAALFSSNYAAPMLAGFVNDALGWKWTIIIAVIFAAVCFVLMFFLMEETNYERKLRVTRDEEGNILNVITSNLSGENTDPLVIKSVTSRVTERQHLGNQAFLIDDSVPIEYAPEKTFWQKMSLTSGFKKDFHLWEYMIAPFYMVQFPIVLWSGFLYGSSLFWYSVLNGTEAIVLGAEPYNFSAAMCGLAYVSPVIFVFLIYPYAGWSTDWIKIFIAKKHNGISQAEDRLWVLIVYMILGPAALILWGVGAAHGVHWFGVVVGLGLQAGLCIVGCISSVTYTLDCYPEMGVSAITVLVIIRNTMNFAMDYGITAFVTNMGVQNCFITSAFICFFCIGTFFVMIYTGHYWRNRTKGQYWKLVSYYRSKGIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM60</accession>
    <name>A0A1E3NM60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Xaa-Pro aminopeptidase P</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_16505</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017781.1">
        <property type="nucleotide sequence ID" value="XM_019161229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM60"/>
    <dbReference type="STRING" id="763406.A0A1E3NM60"/>
    <dbReference type="GeneID" id="30177916"/>
    <dbReference type="OrthoDB" id="9995434at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000009">
        <property type="entry name" value="xaa-Pro aminopeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000003">
        <property type="entry name" value="Xaa-pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="8">
        <location>
            <begin position="87"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="7">
        <location>
            <begin position="403"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="9">
        <location>
            <begin position="630"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="37"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="693" mass="78930" checksum="71D475B8FA9A0E52" modified="2017-01-18" version="1">MSYVQEKWSPAKEKQLIESPDYKTSCLSTLKSFLSFSSGGSGSSNSSSEDGSDMDGTSDMRHAYSFSSNDDDVQPIRIFSQFDSTQRLKRLRTLMKLNNLTAYIIPSEDEHQSEYTSVKDQRRAYISGFTGSAGVAIVTLHSAALSTDSRYFLQASEQLDGNWTLLKQGVKGYPSWMVWGIDECVYQMDQMGETGDIGVGPRLITWSMGNDLIRKTHENNIRLVNDLDKNLIDAIRGKLKDTGNHEVSIYPYKYSGMDTSDKIVRVRKMMQQEGCFAYVSNMLDSIAYLLNLRGNDIIFNPVFFCYVIIYMDEVVLYIDKKKLTAEVQTYLSDLRIDVRVYDQIWNDLPAITNKSPEEKESNKICLELNSSYSIYINIPSTYEIVFRSILTELKGVKNDIEMENLRESQTIDSLAIVRFLSWFDANHPKRRLNELDVVDKLYEFRNKSLKFKGLSFETIAASGANAAIVHYEPTSDNFGEIETDNVLLLDSGGQYLEGTTDITRTTYVYRDKKPSDELRRSYTLVLKGHLNIAMLRFKFGTSSYYIDSLGRAPLRQYGLDYGHGTGHGIDNYICVHAGPCGLSPSRTSYNYKPLEAGNFISDEPGYYKAGEYGIRIESDVLVVNGDDGRLQFEYLTMVPFCRDLIDLAYLSSEQVKWVDEYHRTVYETLVPHLEAVDDVLAIEWLRRATEPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM61</accession>
    <name>A0A1E3NM61_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_25890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018345.1">
        <property type="nucleotide sequence ID" value="XM_019162418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM61"/>
    <dbReference type="STRING" id="763406.A0A1E3NM61"/>
    <dbReference type="GeneID" id="30179105"/>
    <dbReference type="OrthoDB" id="121932at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018607">
        <property type="entry name" value="Ctf8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09696">
        <property type="entry name" value="Ctf8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="115" mass="12284" checksum="554464AC2E24F060" modified="2017-01-18" version="1" fragment="single">TVVATPLGLALVEIQGELSIPKETPAGLSSPESREGGGSGDAEDVEVVKFGRLELDEELQRATLFISTTQRLIGTVEKIDPPLGVLKVVHAGQVCEMIDVVGTKIIFKHRPLPIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM71</accession>
    <name>A0A1E3NM71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018355.1">
        <property type="nucleotide sequence ID" value="XM_019160722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM71"/>
    <dbReference type="STRING" id="763406.A0A1E3NM71"/>
    <dbReference type="GeneID" id="30177409"/>
    <dbReference type="OrthoDB" id="5186at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="22275" checksum="6A35341B28C77B8D" modified="2017-01-18" version="1">MAESQGVDIAFQSVALVSKALSQLESGQLSIMKFGSQSEVVHPFEKQFGGTSGINVFREFKFDDTRTDIKKLVSKSLKVFSDARVFGNSDLWQLEIVLSDGVCEDHETIKRLVRRAREEKVMIVFVVIDGLNGKESILDMDQASYITDDSGKMKLQVNKYLDTFPFEFYVVVRHINELPEMLSLILRQYFTELVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM72</accession>
    <name>A0A1E3NM72_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018358.1">
        <property type="nucleotide sequence ID" value="XM_019163896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM72"/>
    <dbReference type="STRING" id="763406.A0A1E3NM72"/>
    <dbReference type="GeneID" id="30180583"/>
    <dbReference type="OrthoDB" id="2157498at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029164">
        <property type="entry name" value="PIG-Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39400:SF1">
        <property type="entry name" value="PIG-P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39400">
        <property type="entry name" value="YALI0E29227P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15159">
        <property type="entry name" value="PIG-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="215" mass="25736" checksum="A2C3A53B2B866E63" modified="2017-01-18" version="1">MAHPYDQQTAARFKSKTQIGLKAWGYSYSFLEKRRVSPTIMQNENRERGSRHQVVIQPPQENTCRKKRRTEDIQFQYRQHYRTTSSYSSKDERALLKDVFESDEAYEQQQEYLRNLKVSGYLIIIITWILFVFSIGTIFNLWHWCFNFNPQYMRKLKSIPWLKVLIEDISQQNDSAAVPNYYLYLFFLSFVILWIWAVASWISMKLFRHSKGGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMB4</accession>
    <name>A0A1E3NMB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tyrosine-protein phosphatase CDC14</fullName>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_16569</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017851.1">
        <property type="nucleotide sequence ID" value="XM_019161261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMB4"/>
    <dbReference type="STRING" id="763406.A0A1E3NMB4"/>
    <dbReference type="GeneID" id="30177948"/>
    <dbReference type="OrthoDB" id="5632at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120105">
        <property type="term" value="C:mitotic actomyosin contractile ring, intermediate layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071958">
        <property type="term" value="C:new mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030869">
        <property type="term" value="C:RENT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071470">
        <property type="term" value="P:cellular response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140013">
        <property type="term" value="P:meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051229">
        <property type="term" value="P:meiotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044878">
        <property type="term" value="P:mitotic cytokinesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031536">
        <property type="term" value="P:positive regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903501">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140429">
        <property type="term" value="P:positive regulation of mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902846">
        <property type="term" value="P:positive regulation of mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031031">
        <property type="term" value="P:positive regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14499">
        <property type="entry name" value="CDC14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17657">
        <property type="entry name" value="CDC14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000038">
        <property type="entry name" value="Tyrosine-protein phosphatase CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044506">
        <property type="entry name" value="CDC14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029260">
        <property type="entry name" value="DSPn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14671">
        <property type="entry name" value="DSPn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="182"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="254"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="429"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="505"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ46738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="540" mass="61631" checksum="13034D5F235A1610" modified="2017-01-18" version="1">MGKEEIAPIEFLKGKIYLVVSDEKPEDTDEFVYFTIEDSLPYNNFNLDFGPLHAGHLYRFAVALHKILNEPKNKSKCVGFYSSNDSRQRTNAACILCCYMVLVQNWAPHQVLLPIAQVDPPFMPFRDAGYSHADFELTIQDIVYGVWRAKERNLLDMRSFNLEEYEQYERVDQGDLNILANDFIPFASPKQSRPGAPLNEPFKKVLEFFTEHDVKLVVRLNSHLYDKREFTKKNIQHVDMIFEDGTCPSMEFVQKFIGASETVISKGGKVAVHCKAGLGRTGCLIGAYLIYTHGFTANECIAYMRMIRPGMVVGPQQHWLYLHQNEFRDWRHTMVLDTIPDEFIGGLFPLISYEEYKKREQEYEANPSEESSDDQMNFKNDQLIRTPVRRRKISNGLKIMTKAVPMASPGQPRKYRSLDNPVQAYANSDEEEAYTSTINQTHNQSTIEDKENKESRSNNGSPYYKKTTTTTTTTMIAPASSPDTSGRVKEDPNFLKIGKQRKTIVHAAKPRSRVSSSEKIIGGSGVRKASSSKKTSYVHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMH5</accession>
    <name>A0A1E3NMH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_111250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018438.1">
        <property type="nucleotide sequence ID" value="XM_019159756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMH5"/>
    <dbReference type="GeneID" id="30176443"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="92" mass="9917" checksum="9C9C586FCD6E3027" modified="2017-01-18" version="1">MQPAQAGRCAFSARRWICVARCTSRVLLCRRIGCGAAFVADTACAIMARLSEAGPLERPRCSPDRVYAPNGKIGCSSNDRNNAARGSSRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMI8</accession>
    <name>A0A1E3NMI8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_111427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018450.1">
        <property type="nucleotide sequence ID" value="XM_019159761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMI8"/>
    <dbReference type="GeneID" id="30176448"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="202" mass="21601" checksum="9AF4C6757DACF0DF" modified="2017-01-18" version="1">MPNFPTQASQTTMKDPDLHTRAGADPRPVIYCSHPVAAAPYPEVSSTCHLCLLPSCHSPPWPSSCPYMPDTAHFPARKAPTPATVRHVHSPPASPADLIPPTRRCSRFVSQCPPASLRPPPSEPANPSATTPPLVPAATGRPRRCSAASAPRYVRRCGRAPYVRKPGASFRSGFPPSHVDSAQWAISRLKGDHPTLVMGSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMJ7</accession>
    <name>A0A1E3NMJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Non-structural maintenance of chromosomes element 1 homolog</fullName>
            <ecNumber evidence="4 15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_71427</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the Smc5-Smc6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the NSE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4 15"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018460.1">
        <property type="nucleotide sequence ID" value="XM_019163920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMJ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMJ7"/>
    <dbReference type="GeneID" id="30180607"/>
    <dbReference type="OrthoDB" id="185455at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011513">
        <property type="entry name" value="Nse1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014857">
        <property type="entry name" value="Nse1_RING_C4HC3-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973">
        <property type="entry name" value="NON-SMC ELEMENT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07574">
        <property type="entry name" value="SMC_Nse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08746">
        <property type="entry name" value="zf-RING-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Non-structural maintenance of chromosomes element 1 RING C4HC3-type" evidence="16">
        <location>
            <begin position="255"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ47347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="309" mass="34909" checksum="CDF753ECA85CFC5F" modified="2017-01-18" version="1">MSEVVPHEFMDLHTGEKSYTALHAAMVDLVMSVRTIRQTSLLLYLKKALVTLYIDQEQDPSTIESLRSLVQSDDPLPDVDLSILSTILTSINTRLEPLDMEIAESKDMDDSSVNIFSFVNKKPTGAIRLSTKYSQNDVQLVKQVIERIFASEYVNSASATDEEGTVHPRITFSVPCMKMIKYLRDGPRQLDDDDAPVINRFTLEEADLFLKDLECYGWLERNLNNYTLAPRGLVELKKYLIDTYGKCPEGTVSVCYGCGDILTRGIACSNASCHVHFHRYCCELVQKSRDDNSCPNDGCSETFESFYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMR2</accession>
    <name>A0A1E3NMR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Oligomycin resistance ATP-dependent permease YOR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_15376</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018538.1">
        <property type="nucleotide sequence ID" value="XM_019160798.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NMR2"/>
    <dbReference type="GeneID" id="30177485"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001750">
        <property type="entry name" value="ATP-binding cassette transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="818"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="851"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="930"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="169"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="516"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="818"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1132"/>
            <end position="1385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ47425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1396" mass="157028" checksum="5E7996E783E62DAE" modified="2017-01-18" version="1">MGISTLFSTKRTKSIHYADEDIERRNARDSRIHPQHRLLTPFLLDKRVPPIPTEEERIEFPFRKTNLFSESFYLWCLPLLSVGYRRTIQPEDLYKIPRGSTYDVESRTKIFLGHFYAMKERAETHHMERYHIEDTPENREQLQFSPDFKYPRYLVLLALYKTYFFNFTFATLCKALADVASALNTLQIKALINYVHKKALHEEVGKSGYGLAVGVACVVFVIGLLYARNFNDASFCGAEIKGVLTKVLLDKSFRLSKDSRTKFPSSTVTAYLGNDLSKIDLATNFFSFIVCLPIGLGITIALLAVNLGGASLSGIAWFILITGFIMYSTRYLMRWRKSVNVQTDARVKHTKEVLINIKMIKYYAWEIPFTKLISKLRSQEMKIMLKVQFFRNMITGIAVTLPMCSALIGFLSMYGQLGGLKNASNIFSSVTLFNILTMHVAMLPTALTSASDAWIAFTRVQEFLLAEESKPDVNYHKTEYNPQKPAINIKNAAFDWLKPSDDTCSVGSGLTESVTSEKHSLTDELTEKQTLNLHGIDIQIKHGEFIVITGTIGSGKSSLLAAINGTMTRVTGAVDISGDLVLCANPWIQNTTVRENITFGLDYDRETYQTVIESCALPSDFEILPAGDMTEIGERGVNLSGGQKARISLARTVYRAYTLKEYNIVMFDDVLSAVDAKVGKHIMQECILGLLRDKTRILATHQLSLIGEADRIVYMNGDGTTDIGTHEELVERNDRFRALMDFQMEGEVKAEEDEDDEDLDVKESELKLIRKQTTKDERQGRMIQVEHIKSNAISKTMFAAYLRSGCGKLGPRIVLSNLLCAIILTSFCMLFENVWLSFWSLRKFKAYGDPFYIGIYVMVTCFFVLCAIWQFCTIAFISNRASKLLNLSCLRNIMYAPMSFFDTTPLGRIMNRFTKDTDVLDNEIAEQARLTCLGFGNVIGVLILCIIYLPWFAITIPVLFLFVIVIFSYYQATSREIKRIEGAARSFVFSNFDEALQGMSTIKLFSASRRFMDRNSKNINVMNESYFAAVCLMRWFSIPLHGAAGSISLVITMLCVSNAYPISAAGSGLLISFAVQFSMQIISSTRALGQLEQLLSSVERICEYATELPSEASYNTPESIELPKSWPQSGAIKFNKVSLKYREELPLVLKNMTLDIKAGEKIGICGRTGAGKSTIMTALYRLSEPEGGNIEIDGVDIQSIGLFDLRSRLAIIPQDPVLFRGDIRRNLDPFGELSDSVLKSALDIASGYASSDHDKDSEIDIIQASDSNTDNEKKFSLDTFVEDDGANFSLGERQVIALCRALIRNTKILILDEATSSVDYETDARIQETISNGFRDCTILCIAHRLRTILNYDRILVMDKGECAEFDTPKKLWMAEGIFRSMCDKSGIDENDFQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMY5</accession>
    <name>A0A1E3NMY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 55</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_20696</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OB-RGRP/VPS55 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018101.1">
        <property type="nucleotide sequence ID" value="XM_019162170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMY5"/>
    <dbReference type="STRING" id="763406.A0A1E3NMY5"/>
    <dbReference type="GeneID" id="30178857"/>
    <dbReference type="OrthoDB" id="14246at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034424">
        <property type="term" value="C:Vps55/Vps68 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007262">
        <property type="entry name" value="Vps55/LEPROT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050">
        <property type="entry name" value="LEPTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050:SF0">
        <property type="entry name" value="RH04491P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04133">
        <property type="entry name" value="Vps55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15131" checksum="B49644838D9484C9" modified="2017-01-18" version="1" fragment="single">SPLTTLFSLSLLLALGFFNIVLSCALYDTYYPLFVILVFLLAPIPNSIAGCVANSSSYYGGYGDMDGSDPVSNFEAFMKFLTAMLVSSGTFLPIVLWRNDIIPRGSFLLSLTGGFLVYLSFVLFGMGFQTSDEDNYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMZ9</accession>
    <name>A0A1E3NMZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">25S rRNA (uridine-N(3))-methyltransferase BMT5-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018605.1">
        <property type="nucleotide sequence ID" value="XM_019163953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMZ9"/>
    <dbReference type="GeneID" id="30180640"/>
    <dbReference type="OrthoDB" id="273345at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070042">
        <property type="term" value="F:rRNA (uridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019446">
        <property type="entry name" value="BMT5-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF26">
        <property type="entry name" value="FERREDOXIN-FOLD ANTICODON-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10354">
        <property type="entry name" value="BMT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="25S rRNA (uridine-N(3))-methyltransferase BMT5-like" evidence="2">
        <location>
            <begin position="82"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="371" mass="41706" checksum="6D4E7AE1E2E50CAC" modified="2017-01-18" version="1">MAKKGKGALASALARHQSEQQQRKTKLQKLNNKEIHYPGNKKKPKSKTVAADASKKDEEAASETPLDEKKVLVPFDKKDRILIVGDGDFSYTLSIVKKKLIKAKNVIATSFDTLDELHSKYPDTVDATLQELRELGVTKVYHGIDGTRLAETLGVNIRSKRQGDGSGKSIEVLGGLTVNSIIFNFPHIGKHISDVERNIIKNQELLSRFFGSCREFFSILRRQRELRTRSGYKALEQDDQDKDKDSDEDEDEDGDGDAHGAYFSALGKKQQPRDSDVITVTVFSGEPYDSWKIKKLARDSIGYSVQRSGRLEWRYYDGYSHRRTAGAGSTNKRASTRDARIYKFEKWDAAAHARTKKRRRGDASDTDSDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN06</accession>
    <name>A0A1E3NN06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase II-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16608</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the RPAP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017896.1">
        <property type="nucleotide sequence ID" value="XM_019161275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN06"/>
    <dbReference type="STRING" id="763406.A0A1E3NN06"/>
    <dbReference type="GeneID" id="30177962"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051966">
        <property type="entry name" value="RPAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025986">
        <property type="entry name" value="RPAP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423:SF1">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13877">
        <property type="entry name" value="RPAP3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-polymerase II-associated protein 3-like C-terminal" evidence="6">
        <location>
            <begin position="217"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="327" mass="37254" checksum="CAB6DE11102657E6" modified="2017-01-18" version="1">MSIDSPNKLKLEGNEFFGRKDFESALSKYTKAINLDEANPILYSNRAATYLNLSNWKRAIDDCDTGLKLVGRKDIAALSPVEVKLLCRKAIGLRNSDKPKEALKCINDALLIDPTNKALKSEWHLLVRNEQLAESNFGQITNNAHLVTVDIQEVDEIPKDFFSQNPNAEEISSEISNEGSPIRTEAHEEYHSASERMTVSGIDNHTGTPEYPSHPTVQFLSALRLQPETKRQSYYKYVLSVNPQQYRDIYKMTGLDPEFLNFFLEACVYALQNDHLQYKESILNLLSLFASLPRFSLTSMFADSSKIQSLKLLFKNKINKNFGEFWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NND5</accession>
    <name>A0A1E3NND5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_139850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018086.1">
        <property type="nucleotide sequence ID" value="XM_019160167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NND5"/>
    <dbReference type="GeneID" id="30176854"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="118"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="65"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="726" mass="82122" checksum="96C7D52764563D2A" modified="2017-01-18" version="1">MGDCYMVIDKIRYRNVIDNPPAREAKWRTPSEYGNRAEAASAQRLGRSGVQRLVKRSMGCPGRKAGGLGVGVGGAGESKRGHRDSKKTAQRQQSMAVGSTVRSRIRFVGSKTKRSIVSCNECRRRKKRCDEERPQCGACTKRRVDCVYQRDRLEEEVDGGSTVATSGDGQAGKREKESGGQLLAVANAGGGSTVLSPGWYKYMVFSPDGDTDFPPLESVSGSFDAGVDVHLNLGLNPGLSQDIGGLGGLGMKLEMDLDFVARSIIDTFSTARFAAIRMDEGVSAKAADKFLKSCALLLPLAYRSRPVRMVLCAWILSMKGDTRTSQFLDEALGICDRLELKIDYSNDWHEDDLIDYIVSLTCLTIISTINGDTELWKISFERLYTGLRKVGLDMLLLMIKRQENRHVLLWVINWFFYQDVFKMIKVTSNRLLGPLFSKKEYLKFITGGTPPPGVALVEELALSPSSTPPEGDGSSNSSVSTNSEKDFHLKKFFDTSISCCMNLYVVLGEINALYDQFAIKIQYPIFIYYKNVEPAMRNMTEEEQLEFVHTDVYLEYEEIRYGFHSWVQKKTDFLQKRVQECTMVSEGYGPIEEETVAYFEVMKLSVLLYLKFKLKELSATNYEIKQIVLGIFEKMRFLLRHTNGRFNSGQLLFPFLIIGANVCEMRDRIMVKSFYLSMVNSVGSNKRNLEQIWTIVQEFWRLNPDGVSFKMWQNILNKYDWNVCVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNL4</accession>
    <name>A0A1E3NNL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_10652</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018795.1">
        <property type="nucleotide sequence ID" value="XM_019159703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNL4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNL4"/>
    <dbReference type="GeneID" id="30176390"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF62">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="293"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="627"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="655"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="757"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="779" mass="84349" checksum="6016882F3301FE6B" modified="2017-01-18" version="1">MFRSGSPLTLDLKHKTASRSASPTGPSLGRHSGTPAYQDVRLHSTHKNVILVKGSPNESDPVAISGQVVFSLLATTCVSSISLNLRGTYNVHFVENFQASNGKVIATCPFLDSVDVLNCTWDNLLTSSQGRVVNSRAVPATPAVSASKTSSSSATPTSSKGLSILPRNFRSSQLLHNFSSCRTIRYNTDIRLGTTPFAGVDTENTVFELPPGNYSFPFHITLPGSIPETVESMKSVSIVYRFESKINALDPAKTAGSSDDGTTFTAHKYVRVIRTLSSLQLALNEDFLAENSWACKLQYKIRIPRKLVPVGSMLKIYLLIIPIMKQLKLGKITVQIAQYLKFHSSKPNEFGEKTFTDEKIVYQKSLPVVPHSHLPQDVWALEARLPTSDMLKNCSPDVETASHLISVKHKLIIFINLVNPDGHISQIKSKIPFGFYIKPNSRIYGKNVEINKDTGTVAFATGKSLVFDVDSDNNNISAGEDGDIPELIVSMCDELNPDSFLFKDNPNADEIDAAIADTPSPSPALMIPQLSENPNNCSTRCEVPPSYTESANDAVFEPNMWSEGTSPIPGTPRLETPTLEDFSPSSGSSSRLFKIAEDVPCYRKVYDDDASSIGEPAPLYSESHSHASATLRPRASSSSSSVLRSSMEKRHMKTVSASLPSSRPVSPLVSVSSTAFSLRRPPSNTLLNSNTTSSRNNNSSSGNNPPPGWLDACDVAARDSYNEDNSPSSNDGSKDSGENPIVSRHQTPRGQLDRSGLTSCFSSGMLSSSSSSELDLIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNL7</accession>
    <name>A0A1E3NNL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_58086</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018789.1">
        <property type="nucleotide sequence ID" value="XM_019163278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNL7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNL7"/>
    <dbReference type="GeneID" id="30179965"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133430" description="FAD-binding FR-type domain-containing protein" evidence="12">
        <location>
            <begin position="21"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="13">
        <location>
            <begin position="270"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding 8" evidence="14">
        <location>
            <begin position="409"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric reductase NAD binding" evidence="15">
        <location>
            <begin position="512"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ47676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="77488" checksum="E9EEF4AAFF092CAF" modified="2017-01-18" version="1">MKCFVQLLTLVLALVHRCYAEYWTCDTGVALFTFDYTPLGLGSYSYLYDAICGYPPAVGSILICSVELSYDKSEAYQEKIFKTAKKSCDKYSSYHYHWTYYKEQYENATKYYQPLDQISNISLPIYAPTTPNLTSLELEYKGYRGYYFDLDSGTWFSVGICGYFLLLIVISAIHNFFRATGISSSINNSKFSKLCQKYVIFPTLLPNGKFAQEYGWTYFSGLFPNRIQFITDVFLFGLQIAFYCVSYEYKGTWWFGSPDHAWQRFVADRTGIMAFGKIPLLILFAGRNNFLLYITGWSYSTFLHFHKVVALAYTINSLGYYVVYLHDPYFACGVAATVICGVILLQAMHPFRKFFYEYFLTIHVVLAICFIIMCWYHCRALGWLEWLIASCCVWFLDRLIRVIRMSAFGWRTATVTAVDEQIMKVEVPKPAWWFHSPGNYSYIYFAGWIFWENHPFTTVLEGNNLCAYIRVKRGVTYRMWNKLVANNNKLQCKVCLEGPYGGGGSPKLSKYEESLLVAGGSGAPGILENAAKATKGKMIWVAQSLQTVKAYQPLLEKVNIEIDLYLTKQDGSNRACTVQDLFSGSESENLSTESSGKESDSDKNVELKGDHLSNITINYGRPNLHELINASVRDSSSKSIGIMACGPPVMMDDIRNTITENVTTWDKSVDFFDEFQIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNM5</accession>
    <name>A0A1E3NNM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_117881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018802.1">
        <property type="nucleotide sequence ID" value="XM_019159843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNM5"/>
    <dbReference type="GeneID" id="30176530"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="115" mass="12935" checksum="E887A525E09BFB45" modified="2017-01-18" version="1">MTDNRGRALRLTRSEKLPTLIGWPQHGAAENCRQRLICTGRRAAVRPLNDFAAALLPTELCTIGVYVRQRRSLQLPSKISNGRAGPRTLRNGKLHSPATPYLAWRGRQEGQHRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNT7</accession>
    <name>A0A1E3NNT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_32423</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018859.1">
        <property type="nucleotide sequence ID" value="XM_019162765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNT7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNT7"/>
    <dbReference type="GeneID" id="30179452"/>
    <dbReference type="OrthoDB" id="5584028at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37852:SF1">
        <property type="entry name" value="HIG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37852">
        <property type="entry name" value="YALI0B21208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24795" checksum="36B15D5B577340CA" modified="2017-01-18" version="1">MATINPDDLDTTNLNAQSNAEHIQGIYDDIQKIHEESFNSRLGLPAVARVTLFTLGGAIVGGLGGMLGGWTDASLKYLAANSHRLPTSYNGWFFYHKRKTYYCTKNAMGQAFRTGAKVGGFVGIMFSLEAMLDYVRGQADFANTMVATCLPGFTYAWFGKMSKVQARELIHKGGKIGLLLGLSQDALQYIRGIDVWYLNQWFGIKPMKLSERLRQYAGEKGSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNY5</accession>
    <name>A0A1E3NNY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_28006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018917.1">
        <property type="nucleotide sequence ID" value="XM_019162528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNY5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNY5"/>
    <dbReference type="GeneID" id="30179215"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015188">
        <property type="term" value="F:L-isoleucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032974">
        <property type="term" value="P:amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF530">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="84"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="475" mass="53403" checksum="5F201F3E9E58F221" modified="2017-01-18" version="1" fragment="single">ESNLVTRNFLEFLDLYDHFAGQDLEEDDGETETEDEESRNGDENEVIDESEFHHDSASFMRKHNKRRAKQKVGRAQKSKSKEKKVSTPKAFLLLLKAFLGTGIIFLPKAFSNGGLLFSNLMIVSFSLISYYCFSILIRTTSRCKVSGYGDIGFKLFGKKMQFAILLSLVLSQLGFASTYTVFVCENLQQILQIVYGRIYELGFFFLLQVLIFVPVSLTRSISKLGVCALVADVFIFLGLLYIYGQSAVKLVSDGPSEKVAVFKPDTWTLFIGTAVFTYEGIGLLIPIQESMREPEKFNRLLVLVMFIVTLVFTSIASIAYLSYGDDIHTIILMDFPRNSKTNTVQLLYSVAILLSTPIQLFPAIKIVENYIFHQNRLTWRAKIRRNSEAISNCLRVAMVLLMCSIGYLGAENLDKLVSLIGSLTCVPLIYIYPPLLYCKGFAGELRQTEKALSAAVLLAGCVLMAYTSYETLANW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNY6</accession>
    <name>A0A1E3NNY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_71824</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flavodoxin-like protein (FLP) that plays a role in cell wall integrity, oxidative stress protection and virulence. FLPs act as NAD(P)H quinone oxidoreductases. Reduces ubiquinone (coenzyme Q), enabling it to serve as an antioxidant in the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018905.1">
        <property type="nucleotide sequence ID" value="XM_019164017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNY6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNY6"/>
    <dbReference type="GeneID" id="30180704"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="4">
        <location>
            <begin position="6"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="202" mass="21424" checksum="636A9CA00776851D" modified="2017-01-18" version="1">MSAKKVNIVIYSLYHHIAKMAESVKSGVESAGHECQIYQVQETLPESLVKKLGSPGKLPYPISDLNTLTECDGLTFGIPTRYGNMPAQLKAYFDSTGSLWAKGSLKNKPFGLFVSTGTGGGNEMTAVTALSTFVHHSMIYVPLGFASVFPQLTNLNEVHGGSAWGAGTIAGADGSRVPTALELEIAKIQGKEFAEVTAKLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPF2</accession>
    <name>A0A1E3NPF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">LEM-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_71642</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018701.1">
        <property type="nucleotide sequence ID" value="XM_019163971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPF2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPF2"/>
    <dbReference type="GeneID" id="30180658"/>
    <dbReference type="OrthoDB" id="2503928at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12935">
        <property type="entry name" value="LEM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025856">
        <property type="entry name" value="HeH/LEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044780">
        <property type="entry name" value="Heh2/Src1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018996">
        <property type="entry name" value="Man1/Src1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN HEH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808:SF2">
        <property type="entry name" value="LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12949">
        <property type="entry name" value="HeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09402">
        <property type="entry name" value="MSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="HeH/LEM" evidence="8">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Man1/Src1-like C-terminal" evidence="7">
        <location>
            <begin position="444"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="672" mass="75975" checksum="2D88A0940C24AD63" modified="2017-01-18" version="1">MLDPQEYLAPEYDIGKLTVANLRSILVEHEAAFPSNANKSQLINIFNEKVKSKKAEILKRYDVGASRNDIIDMTSGNDSRKRESLGSNEKIHLKEPSPVSEKDKIIPSKRHSDPTSPQKRNKRKTRKRVQSNSLSEDSSASPAASFLSMEKFEIDENDNIFRGPIDNTPITLLNKTKFSAKKSKVPVKDILSKFDKSVPSSKVFISGSTESKAKSVSPSPVKDSILSATKPVDTANQTVDNAYLTSTAVDTASILASNDYLPNRIENISAGIDQELNNGDVNDPQTSNTFDYKNDNDAAILINDIFSSNERSKQLNGPTKNIKAAEPVINIPSSDDDEAEVIEDKADEHLVSTLDVKSFSSLDTDDVSFSRESVDIEGKDEANEQESICHEMVLEGIDETETIEGKTDNENVKTELDTMVTRSTISKFSHVFKTLWKSFLLTSILAIAFFSFSLREIKNNTGYCGFEHHGKTLNLWNRLPDSLQNNLQPAKPYIELLESGMINVAHFECEECPENGTCDLNKLTCDYGYVKTYTWRSAFGLVPLQETCEYDYLREEKMRYLSKYTLSYLHRHNDKQLTLDELHDYLRSTKPSSMSVDEFEGYWRSFVEHELSQEPDLSINFNTKEITLSHRTPTEFYTRTFGNVQRGKKSKNLFKRTPPTVDLKNYYTKAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPL5</accession>
    <name>A0A1E3NPL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_58385</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019183.1">
        <property type="nucleotide sequence ID" value="XM_019163294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPL5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPL5"/>
    <dbReference type="GeneID" id="30179981"/>
    <dbReference type="OrthoDB" id="19981at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="335" mass="36340" checksum="8558BDBE07C1A886" modified="2017-01-18" version="1">MNYTFGDRVKKLVNASAIDKSEKDAILSLGRITYKELREAHQKCSGEPDVGSLMHYMSDLTPVFRVYDREGLAKAAKTDEFKALMSRLRAEEKEREYRTLLKRGHDAGAHVDGIGSYAFLAGRGQAGSAGQAAKEVKHQLTTIVNILITVVSAGYAVWYWSGSSMGLSRDAGAAGNMGVRVLLSLFAAVLVLVAEIVVFGGYLRKVDDARTKEHALVEDRSVVQTIVIKGKGATASSGPTRDRASIGKKRKRKGRAAYVSAPTRRDTDQIASFLRQQRAFGEAGTPEAILAARGAEQQALRCGGAGTAEKRCSCIKRKPIPLLAWSGQAWPCRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ27</accession>
    <name>A0A1E3NQ27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Stretch-activated cation channel Mid1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_20389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019322.1">
        <property type="nucleotide sequence ID" value="XM_019162158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ27"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ27"/>
    <dbReference type="GeneID" id="30178845"/>
    <dbReference type="OrthoDB" id="5405745at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024338">
        <property type="entry name" value="MID1/Yam8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142:SF1">
        <property type="entry name" value="AEL197CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142">
        <property type="entry name" value="MID1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12929">
        <property type="entry name" value="Mid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="47280" checksum="1F85A825D330274A" modified="2017-01-18" version="1" fragment="single">NMTNLAESIPVRDSVNYSTLVAYKFEPDLDSEWTHSYQIFVYISASLCGLPDTWDTDLSENGLSLYYTFNETVAKSTDTDEMTAIRFRNGYAEGLAQMQMQDNVSKYTLYMLVIPDSCDECTRDSNWVYEFAVSQRNLLFLYDVEPFISVVDVDYDTVIFEAGKINFGKNRSYGMYIFEDGNYIPTSLNQSWCAISESTGYGTRVEIQENTTESSRNEFLVSGLEISKHYSAVLVVTFTDIPYGGGVFKQFEFTMSTSKACKLAYSLGFCNEVAYAVPVSTGFYDGDESWEQLMAAYDNYSESLYTPFGYAMQQIPCDTELSARYSPIRTCDDCKYSYKQWLCSVTIPRCISPSHAGPQNMVYDAGSGRNHFIEQTIIPPLPYAEVLPCLNMCEAIVRDCPPAFGFACPQSADLVQLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ95</accession>
    <name>A0A1E3NQ95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019001.1">
        <property type="nucleotide sequence ID" value="XM_019160952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ95"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ95"/>
    <dbReference type="GeneID" id="30177639"/>
    <dbReference type="OrthoDB" id="2281895at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040030">
        <property type="entry name" value="Ribosomal_mL57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28160">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L15, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28160:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="106"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="254" mass="28621" checksum="7E2FC6918CAF5658" modified="2017-01-18" version="1">MQSIINKPVTIMAYEHTARGLQLRNLSKISQTRLYTINHHNTHRANTHQRLTITQQQHYHHHQQQQQRSIVFVRGQRVQGLIRDPSEVLSSDGVKYGAESDNLAPLKNYLSSLNLSTKIELPDELLLQIITHKSFAHGSKPYNAHISFLGEQILQLCATKHVVGKSKNLDAIGSLAHRIMWSDKLLAAFAESKNIDKVFFCKKALPGGNIDKLYKPKSIYSTITSSLIGAIASKYGKNAAEEFIEKELIPAYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQB9</accession>
    <name>A0A1E3NQB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein-tyrosine-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_11156</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019365.1">
        <property type="nucleotide sequence ID" value="XM_019159764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQB9"/>
    <dbReference type="STRING" id="763406.A0A1E3NQB9"/>
    <dbReference type="GeneID" id="30176451"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="209"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="35860" checksum="6E7523F295771577" modified="2017-01-18" version="1">MKSIPKGLLHSRQQLIHEYQELQKIDRSIYSREKKSLNHRAENRYRDVIPYESTRVKMQYPTKYTVDGYINASFVSPDGSDVHRYIASQGPLENTVGAFWQCVLEEIFLHDKIVVNVIMLTELKEGNHEKCFDYIKNIERDVPQAMHDVFNVDFDQVKFSGLQTLNEGGIELREFVVTTTGGQEKIARHVWVRRWPDFGVPSRLSDNNEYVIKYLSDADSKAGVSRNEVSSVVHCSAGVGRSGTYIAMDWYYNEVFQKQDKEMESRDDVVFSCVANLRKSRVMMVQRFEQYEYLYHALRDFYGSDEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQM2</accession>
    <name>A0A1E3NQM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">rRNA-processing protein FYV7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71868</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FYV7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018956.1">
        <property type="nucleotide sequence ID" value="XM_019164033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQM2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQM2"/>
    <dbReference type="GeneID" id="30180720"/>
    <dbReference type="OrthoDB" id="2135053at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013730">
        <property type="entry name" value="Fyv7/TAP26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08524">
        <property type="entry name" value="rRNA_processing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="189" mass="22195" checksum="DF59BB3D494DC4F0" modified="2017-01-18" version="1">MSGSIQRGGRSNRGRKDTRKYLQGKQGKTDEIRRALTHRARLRKNYFKLLEREGLDVPQRGTTGNESYDNGKKDVSSGEESVNEYEKTEDGSGKPRKPLSEIDIIKDKVKKHESLTFQERILLKKDRREKDRERKMNKTKEKVESMKLSDLKRQRQTERIQNTKTRKGQPLMGPRINNLLDKIRQDKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQS1</accession>
    <name>A0A1E3NQS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_177940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019515.1">
        <property type="nucleotide sequence ID" value="XM_019161745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NQS1"/>
    <dbReference type="GeneID" id="30178432"/>
    <dbReference type="OrthoDB" id="3980884at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="439"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="491" mass="57077" checksum="2C312472F29E736A" modified="2017-01-18" version="1">MSDRRSSRSVFRPIRDRFVNAWHHHVSKKYPQEASIRNPYSRTHADVSVIPPAVESELRHMFPLTTDNSWEPPVFNESFHRSATYDASSAMICPFPYPYTGPYPCHNPNPDFRFTTSDTVFDSAYFLSNDIETIATILEQVYANNLKFLVDNPYWNYGICPPLMENLGIANKYIVSQLKGEPLVTLDLHFPEFTNLLASLQYDNKIQRVLCEQKKKGENMRFLQAYKFDDKKTFIKNGLVPVVIVGIFCAEIPVSASYEYVPVDANAKRTIKFVDRNHIFHEFNTNITRLEQQISFSMKQYPIFNELTPYKGPRFSMRPIIFTNLSEYGWQLVQLTHIFQFQDPILKALPNLLKQMYNYLSNPVKQNGSPPHSPAPKIFESPDLTLLMSELTDHYTVPNMDLYLKQKPWGRPSLVSFNINSDTHIDAQYLTQLNQGDECAICLLEYKECEICLHLNCEHVFHKSCIEQWINSSNDSRCPLCRRINKRFGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQY5</accession>
    <name>A0A1E3NQY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_31908</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019231.1">
        <property type="nucleotide sequence ID" value="XM_019162730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQY5"/>
    <dbReference type="STRING" id="763406.A0A1E3NQY5"/>
    <dbReference type="GeneID" id="30179417"/>
    <dbReference type="OrthoDB" id="1697690at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:7770001">
        <property type="term" value="C:mitochondrial pyruvate carrier complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050833">
        <property type="term" value="F:pyruvate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="120" mass="13306" checksum="B28EB82066972A2A" modified="2017-01-18" version="1">MSSPVTNSAAQKFSQKVFSKETLKYVFTTHFWGPVSNFGIPIAAILDLKNKPEETISGPMTAALVVYSAVFMRYSLAVTPQNYLLFGCHVVNEIAQLGQGARFIHYNYLSSRPAAVEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQZ9</accession>
    <name>A0A1E3NQZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14066</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019618.1">
        <property type="nucleotide sequence ID" value="XM_019160207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQZ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NQZ9"/>
    <dbReference type="GeneID" id="30176894"/>
    <dbReference type="OrthoDB" id="26740at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21675">
        <property type="entry name" value="SMP_TEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019411">
        <property type="entry name" value="MMM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF19">
        <property type="entry name" value="NUCLEUS-VACUOLE JUNCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10296">
        <property type="entry name" value="MMM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15413">
        <property type="entry name" value="PH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="328"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="671"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="756"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="867"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="758"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="898"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="922" mass="102416" checksum="D964CE76A6232189" modified="2017-01-18" version="1">MGFLSYVLTYIVGGVTFIPLIIVLIWYKSPQVPNSVSERDRKDAIKAERESLEKGEPKGYRDLKAGEFFDKEKLGIKAYHSGWITVTKEFYRFPQINPEEFKQTNTFNSDGDLSNGSAGNALGNNASGGIFSKMMKGGGGGGNGSHSHSNSMDEEFKEGEQDTATLGAAKLKQIRKRNRFYGVIKHGNLFLYSDETQKNVKHAIVLDHYTVAIWPRNLRDGQLFTKRSAICLINTDELAHSKEKAQELLKLLKSKSDNIPPPKNCYFLYADVSIEKEDWYFAFLRATCTNNTLSKLNPNFDPTIMAKPAYYNTADMLDLIETLNATEQQLTTKWINALIGRLFLSTYKTDEFKAAFWLKIEDRLKKIRTPGFLDQLQINRIDVGHSGPFFTDPKLKTLSPEGDLEVLVNVSYQGRAMVEIATKLFVNLGVGFKQRQFDIVMKLIVNKIEGELLLKIKPQPSSRIWYTFSKMPEIDITIEPVFSSRAVSYGIITSIIESRFRDAIKSSLVYPFFDDFVFFRNPEEIFRGGIFDRSCRKSTTNDEDIKMAGDVVKDSISTLPPIAQPISTDPLSNSGEAGIKMEPIKSSNASVISRASSTTSTGASKFSEKDHVISKLSTISSEAEHYNEPTSNSELIEDDVSHTSELKDSVLKSYSRIKHWYKKSPAVPDTSGPAAIGSSATLPRRKTGSIGGKDPNYIPPEMISNRRIKPSKPENLQLDLPSQSDTISSGSSFQLLNSNINTRPSGDAFINLDRKRGTSQSSQFGASSFTEDNPMMLPLNNNNPPSSPEMFINEKFKSPTTATSNSNNSSVMNYAGNKNRVISSPSILRFSNDNDDSLSSPTAASVAALSAGLQESPILQFDGTNDFQKQDTERQQTPNSGEFGAPLSRPRTRLARKPVPPLPPLPPKDNEFSLPKNSSTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRD5</accession>
    <name>A0A1E3NRD5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetylglucosamine-induced protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_19916</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019361.1">
        <property type="nucleotide sequence ID" value="XM_019162147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRD5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRD5"/>
    <dbReference type="GeneID" id="30178834"/>
    <dbReference type="OrthoDB" id="10053431at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022036">
        <property type="entry name" value="DUF3605"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020:SF2">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12239">
        <property type="entry name" value="DUF3605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19822" checksum="4A283CE30FFDB938" modified="2017-01-18" version="1" fragment="single">WSQITHLVKTYQLERLGRSQSQLKSYHDFKEQMAQQGICLTTNLLVNTMHWLPADTDIRLPAEEAVQRVTYKDARLFAAAEDVYISINEFPYYIQERTLHLLVWVRSPMPPDPASDIGDIDSTTKETIERYVMATFVRKAGVPRDHLVWWKNYTKIQSIKAIPHVHVLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRG0</accession>
    <name>A0A1E3NRG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-alpha-acetyltransferase 40</fullName>
            <ecNumber evidence="4">2.3.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_70287</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-seryl-[histone H2A] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H2A] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12742"/>
            <dbReference type="Rhea" id="RHEA-COMP:12744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-seryl-[histone H4] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H4] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50596"/>
            <dbReference type="Rhea" id="RHEA-COMP:12740"/>
            <dbReference type="Rhea" id="RHEA-COMP:12743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. NAA40 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.257" evidence="4"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019779.1">
        <property type="nucleotide sequence ID" value="XM_019163601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRG0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRG0"/>
    <dbReference type="GeneID" id="30180288"/>
    <dbReference type="OrthoDB" id="424551at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043998">
        <property type="term" value="F:histone H2A acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039949">
        <property type="entry name" value="NAA40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="41"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="232" mass="26358" checksum="9D28B35DFE64A87E" modified="2017-01-18" version="1">MSDDIEQKESKILAQFAKGVFSAGLLKQSCPDVPEVTVDVIRVEKLSETELEECLSLLDRNLGSIYALVNGKGWKKPKRKEMIEPGLVYVLLRAAGTNKLIGLISMKIVNEYDLKVLYLYEIQVDESCRNKKLGTFAMLQLDRLVLMINSSDKLKQLWLREYEGEYLGLHDPEILLTGVALTVFSVNKGARKLYQRLGYKLHPDSAKDRVLRSGKVIEPIYYMLEKSVKDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRG3</accession>
    <name>A0A1E3NRG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019266.1">
        <property type="nucleotide sequence ID" value="XM_019164109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRG3"/>
    <dbReference type="GeneID" id="30180796"/>
    <dbReference type="OrthoDB" id="4207594at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF56">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="85"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="171" mass="19616" checksum="51DFE08455D78207" modified="2017-01-18" version="1">MSQLPYDVTKEQLSQAFERYGPIEELHLQMAADREHTKGHGRMVFADERNARRAAVQMQSYVLNNMPIKLTLGSEQRRGQATRYDVVMLRNLPHDASEDELMKVLRPYQALRVGLPRSPVTQESLGYGYVRFGSAEAAERAVRELKGTSVGGRRLRLHVAEPKKHHYRFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRW4</accession>
    <name>A0A1E3NRW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein mL38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70411</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphatidylethanolamine-binding protein family. Mitochondrion-specific ribosomal protein mL38 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019922.1">
        <property type="nucleotide sequence ID" value="XM_019163631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRW4"/>
    <dbReference type="STRING" id="763406.A0A1E3NRW4"/>
    <dbReference type="GeneID" id="30180318"/>
    <dbReference type="OrthoDB" id="2153661at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000004">
        <property type="entry name" value="Mitochondrial large ribosomal subunit YmL35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="354" mass="40284" checksum="9FC91B3E274A7B3B" modified="2017-01-18" version="1">MSTTVSRGIWSKFANRSTTLNVKSAALKNALLSPNLPKGPVSLKANASKLKYHSPLGLDKIYPLAYELLEKQSESIYESIVQLDKEAENIKDEAELKRLSQQKQELLAAAEAENPEVVYNSLFATNAVDRTQPVYRHYLKEQWKSYKRMLTMQRLETLAVIPDTLATLEPEVDVQLKFPHTNVDTWIEPGEVLSSNVTSRPPALEIVEFKESTKDLYTVLIVNPDTPDVENDSFTTTLHWGLKDVELTNSDCIIDIKKLDQHPEYTLVDYLPPVPEKNLGKQRFAVWVFRQDGKLDSALAAVERENFDIRKFVAGNKLAPIGAHIWRSTWDLNTDNVRKMYGLPQGRVFSRDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRX5</accession>
    <name>A0A1E3NRX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14389</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019968.1">
        <property type="nucleotide sequence ID" value="XM_019160348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRX5"/>
    <dbReference type="GeneID" id="30177035"/>
    <dbReference type="OrthoDB" id="10261452at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="25"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="426" mass="48076" checksum="AFFAFB7A156AA06C" modified="2017-01-18" version="1">MARRRQKKRTHAKQSEADLKSIPRSMVIHLGTALENNTLTQLVKDTRNMMQPHTAIRLRERKSNKLKDFVVMAGPLGITYLMLFSQNEKTGSTHLRLSSMPRGPTISFKVSEYSLCKDVSHIQKNPKSISASSHEFVNPPLLVISGFTNPKNASPEEKLVVTMFQNMFPPISPINTKVGTIRRVLLLNKDKETGVIELRHYVIDTKLVDVSRNVKKLINVKSHLNKKLPNLSKTKDVADIILDPYAQAGFTSDSEVEDDSIVEVNEGDDSSVLTKPTSEESKLETAQGQKRKKAVKLTEIGPRMKLELIKIEDGVCDGKVLYHSQFSKTKAQMNKLEQQHALRKKAKDLRKKEQAANVAKKNEKKLLKKQRREERKKAGEIGSDESSEEDSSSDEDVEMGDYSDVSDPNDDVEDSHDEKLFDEEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRY9</accession>
    <name>A0A1E3NRY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit KXD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">KxDL homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14372</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) involved in endosomal cargo sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KXD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019952.1">
        <property type="nucleotide sequence ID" value="XM_019160342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRY9"/>
    <dbReference type="GeneID" id="30177029"/>
    <dbReference type="OrthoDB" id="4089816at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051390">
        <property type="entry name" value="BLOC-1_subunit_KXD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019371">
        <property type="entry name" value="KxDL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37787">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT KXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37787:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT KXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10241">
        <property type="entry name" value="KxDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="KxDL" evidence="9">
        <location>
            <begin position="136"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="242" mass="26852" checksum="F52B9D5D7A28946C" modified="2017-01-18" version="1">MASDEGNYDEVTSGLSGERYAVVNREEGLANLEGLSPSSGRPNRSQAGEGGEPILEGHSEMELQRDAAARSHDDENGCEDANIGEENEYDFHDEDEDELVLERIRSSSPVAAHSAEKPSLIPNESRFNPIEYLIGSLNNALDSLAFDRTLVIQSKMAGELNNSANEVLKIVNEMESSLKEHIAKYEKLKQEILPEIESNLRKGNKMANKLTAHVKERYPVEYSKGRSKVLDNLTEDEEGLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS24</accession>
    <name>A0A1E3NS24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS17 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_14381</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019959.1">
        <property type="nucleotide sequence ID" value="XM_019160347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS24"/>
    <dbReference type="STRING" id="763406.A0A1E3NS24"/>
    <dbReference type="GeneID" id="30177034"/>
    <dbReference type="OrthoDB" id="10254436at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000001">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="155" mass="17608" checksum="647F391014111583" modified="2017-01-18" version="1">MTTELTVQSEKAFQKQPHIFTNPKAKATRRTKRWYKDAGLGFKTPKSAIEGTYIDKKCPFVGTVSIRGKILSGLVVSTKMHRTIIIRREYLHYIPKYNRYEKRHKNLAAHVSPAFRVQEGDHVTVGQCRPLSKTVRFNVVKVAAAAGKSKGFSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS53</accession>
    <name>A0A1E3NS53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_27440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019516.1">
        <property type="nucleotide sequence ID" value="XM_019162494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS53"/>
    <dbReference type="STRING" id="763406.A0A1E3NS53"/>
    <dbReference type="GeneID" id="30179181"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="6">
        <location>
            <begin position="35"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="285" mass="31367" checksum="0DC8D727FE4F3F99" modified="2017-01-18" version="1" fragment="single">QSFAPRHLVRLSQMNPIHKRQKMNFKFEMLNPVNIYILDTGINSKHPDFTNRVEVRKPSSKIDDDTGSYEDPIGHGTAVASVIGSQLLGVCKNCNLVSYQILNDLGQGKLKNLIIALTDIYHSEKRGTIVMPFTTPRSELLNDILHEMRVSNFTLVGAAGNLNDDACKYSPASSVDVLTVGSIDSTTDTIAKFSNYGPCVDIFADGINIITLNNDNELLKLRSGTSLSAGITAGLVATFLGLNEVIDGESNFDAIKKIKNTAIENRIKPQEFFKETKTPNRILHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS63</accession>
    <name>A0A1E3NS63_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019526.1">
        <property type="nucleotide sequence ID" value="XM_019160172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS63"/>
    <dbReference type="GeneID" id="30176859"/>
    <dbReference type="OrthoDB" id="3995840at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="462" mass="52767" checksum="E0E42819B6E37B9F" modified="2017-01-18" version="1">MDRVSPSPSISNCSQYKLESDAGSSALASAVRNKGVALQIIDKKGYARDPLSWTCETPQKYWEESDNAKFAVQNKRKEEKPKTDLRSGSRSNSKKNREYRSSYTNKNKEALNPPSESSLGSHLETDLEESDLSASVAHEQSEEGSLSQVPKLSIIPEFKGATAWLKTGMLLDNSANLSAFVEDPFVMQSVVEETRLPEPEPKEKVMLDENVPESVEEIEHLMLNSYKDDESKKIHDSSCLSSGLSSGVGVEDGLSCSYPLKQQSLPSKRDFSKNSKLRFPSKSGGYWIPHVKPLSEFLDFRFEEKVYTFSDQEMEIIKKLDYILACKFCYRGFNIVMMAFMFLVVSVFIMVTILYALEKMPPTFEAIHLMFFDLVVMFVLFAINSCASKEVRVEGQYAVRSYLRIYWHLIRRKFDSSFNKCYMFLDDWYGDDKYKFLKAKLQEISQIEKEISHEADSDPEFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS76</accession>
    <name>A0A1E3NS76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_14009</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019546.1">
        <property type="nucleotide sequence ID" value="XM_019160182.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NS76"/>
    <dbReference type="GeneID" id="30176869"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097308">
        <property type="term" value="P:cellular response to farnesol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF5">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="124"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="1884"/>
            <end position="2105"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="2269"/>
            <end position="2390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1594"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2239"/>
            <end position="2260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="2324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2395" mass="271982" checksum="960113117D273C11" modified="2017-01-18" version="1">MERKGQAEPTGNSAKNNDNANTNGDYFSINRTANLFPHQGLLSSPTSATSYSSTSTIFNLPKYGANRQSSNLNLLLSPIATSPASTNIEPSATESSEAVFSPLSASNTNSLLKDFLHPSVLHELEQKASFFSKHSKSTYLTSSLPHDYLNTSLDLQESASQKSPKSKNDMSQILSSYTLISKIHDDKHSDPINHYLAEHKTTKQKFVIRFIRQLTVGTLCRLVNSYYATYSPDSTQPIDEYPSFKDILEEKNEHRACPPDLPGIYPCTKLELLENQNGIVAFYPVADNVVPLSKMEFDSPVEITTIILLLVNILKVLKSIHKHGIVNNTLLPSDIFVNPSTYDVFIMGFDFSFSTDVDFQEVPYRSANKSHSLNYIPFTAPENFQNLVPVDFRADIYGAGAIIYSILSKRVFKQNNMFILVAAIITLNSTPLHIIDSSIPIEFSKIISKMIDKDAKLRYSSLDHIIHDLVKLYHNITQTNIPHDPVPTSQSRIPILIPSELVGRDEVIKSLSAALSSNSVKTFTLTGETGIGKSSILESLRIPAMTKKMFFTAWKCQDLNYVTSKFQTFDLILSQLLRDILSMDKLDIAFWRDLLSVEVKSDFSLLFDTVPELRILLGPRYAHMKQRKMNKNVYQKELGHQFILKKLFELFSRHAGWVLVIEDIGNLAEAEATVLEELWSHLKNDFKEGELNFTLLTSYTIRGEKTSDDEDNIPSFLRSNVMIEIPRLTYENVERYLSLSLQLYDSQFLWKDMQSKPNSYYFELSSNVRMNQDRRQMAELLHKYSQGNPLVINRFIIQMHFAQLRSKTSLKPIEVFKSIFATIKKTGPSGDVFFDCNLYTKNVPNDHTVQILKYAACVCVGNLFDMYDLSVVTGFDVSTLYIVLSTGIITQTLKSCSIVSKLPVDRLDDPDFPLSKLSAVEKRKLLKYTKFKFFHETIQHQLIQSLKDANELEEFHRNCGLRLFENLEAKYGSLKLTSADCFSIAYHFINSWKVARPEEYSIYTNILITAGWYAYSSYEHVSALRHFQIAKELTTDSQILKGLEWVEIHIYALKGKHEKSIDLIEKALEKYQENVEDVAQFLIAKMQSLRSMNNWEEAYEVCLNTLKVLEFPLDLSSLSGKEIEDYTNFVLRPKLPASPSEIRELRNLPLLKKRRVILVQMVLMDLNQFSVYLNKPYLFPFANLMNVILFMENGKSVYSSFSLIILGSLDATVDAKGLNRAHEYCKLAFSIVSSDSLEANELFIISFKWYCCLVGSLIGPIDKISQSFDLSIINSKAQMVETTFSKGIVVKFKFQLWITQGLTSKNIWERMEVIRDFYRPENDPRGLYDKIYKLVIGLLSVLEGNLSYDKYVDGWLSLGLDLKCVHMGLTYNVSRCFSCYILRKYEVGADIAIRELVGNWSEELASIEMAWARYVFVLIIYKDKVRRCDINDPYSDEISEKIENHLKNTQAFFKELSARNPSVFTCMFLTIDALIKVMDEEAFSQIDILAAFEIALASSVEYHHYLLEAVVAEECARWLKTVSKTNTMTTKYLKIAYNAYKAWGLTLKVEQIEQELILDIDLSLDLSPSPIVRSQRRNSRFYFQNDFIPFQHDSENSSADVSGSFKLPSDKQQDKSFSSYPLNALKAKSDDGIDDGDSYTSSRASVGIPATSVPLRVSDTLVSECGISESNEQDYTDKEMDLNNIEEEDWDNDAVMGLSTKIALSDNFEDIVRMLLLFSIHLVNADYGCFILNSDDGTPYLTAMCLKNAEVRFFTQEILSLKGDVLPVILVEESMKENMSIWRDSDKFYFDTTYKNRDPYFESNNCKNVICIPVKSGDSTVVGALYLENQRESTFVALKKIVLLEYLCLQGYISMSKMKLVEKLEVAKKAAEDATADRTNFLANMSHEIRTPFNSLMSCAIFLLDTKLTKSQRMYVETIRNSAMVTLSIIDGILSFSKIEHGSLLLSFEAFNLTKCIENAVQLVAEQAALKKLELVFIDDNYPVNTVYGDETRIVQVIINLLGNSTKFTNKGYIFVRSSVKEVAKDRYEFTITVKDSGIGIPEGSKARLFKMFNQLDGSSKRVYGGSGLGLAISKKLAEYMGGDVDYESKEGEGTSFCFTLTSKAEKHEAKRIEGIKEDDLVAILDPRELSSLSLKKCVERRGFEAKNVEIFDKLDDAARAKCKKAKFIFIYLEVMKQEHELKQLRGEFPNSVIIYQIPFGVRIPAFIEERDIPGDSEAKLVDFVLLTPFRREKLDEILQNQPQEPGASTGKKPESAKGLKLGKRCPLSILVAEDNMINTKMVRLQLKRLGYESDHAKDGVEAVEKSHQRFAEVGAPYDLILMDLQMPRKDGYEAAEEIKAEFADRTRIVALSANVYSEEKARCLRIGMSGFLSKPLLPDKLSLQLEEAYLQKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSA7</accession>
    <name>A0A1E3NSA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Damage-regulated import facilitator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_14487</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DIF1/spd1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020094.1">
        <property type="nucleotide sequence ID" value="XM_019160398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSA7"/>
    <dbReference type="GeneID" id="30177085"/>
    <dbReference type="OrthoDB" id="4072855at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013900">
        <property type="entry name" value="RNR_inhibitor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08591">
        <property type="entry name" value="RNR_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="161" mass="18300" checksum="2802173CDCE2A102" modified="2017-01-18" version="1">MQVYKRHQRSGFEQQQQQQQSAAPQDATSERLQTLGMRIRQSISNGYKLPEANTSNNAAYINDYEQQQLQNNSRRVPLPAHLGMNGPPALDYNGSTASSLSCWEEELNHSSVNRSQMLNDFYPAQGNPGGVKRGIEELNDKEMQVNDYVQRYGPLSFNEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSH4</accession>
    <name>A0A1E3NSH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_9519</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020135.1">
        <property type="nucleotide sequence ID" value="XM_019165098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NSH4"/>
    <dbReference type="GeneID" id="30181785"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="347" mass="38956" checksum="D373F3423DBC958D" modified="2017-01-18" version="1">MSESTVLVTGATGFIAQHVVDKLLKRKYRVIGTARSEARYSPILEEFKKKYPEGNLTFEVVADIAADDAFDEVLKNHPEIKYVLHTASPFSYGLNKPFDEAYLNPALHGTLNILNAIKKYAPQVTNVVITSSFAAVKQLGDAFKTEIHTNESWNPIKWEEVKNENEAYSASKTYAERAARKFYEEEKPAYNLATVNPPMVLGPQLFDSSVEKVLNTSNEFLNKITHLDPTSTAPQDKFALLAVDVRDVAEFHVLPLENKELESEREFIVSSPLIAQAVLNILNDNFPELHGKIARGDYSSVDDVIARSCPKYDVSSTLKKASDYKLISLEKSVVDVYKQYLSKYSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSR6</accession>
    <name>A0A1E3NSR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_19700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020310.1">
        <property type="nucleotide sequence ID" value="XM_019162136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSR6"/>
    <dbReference type="STRING" id="763406.A0A1E3NSR6"/>
    <dbReference type="GeneID" id="30178823"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="83"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="389" mass="45034" checksum="BBAAEB7DEA86B216" modified="2017-01-18" version="1" fragment="single">HQHSSRRRSKTISSLSSPKLEYNPYGIYSHHNLQSIGSAFGNGNEKNECDLMLTNPTSLPNDYLPIAFQEEVQFLEDKYELSEKSVIFGGSATIRKIYTKADLQQSRFYALKKFSIYTGESQEKYYSRVATEYTITRSIAHLHSIPCYELLQLPVTLQRAWGMVLDYYEYDLYKLVRNPDWKKVAFAEKMCIFKQVCFGLKYIHEQDITHLDIKADNIMVARNGLMKITDYGCSEIGHEEYGNFTSKVACKATRLGTPPYQPPEVAKYGLLDKESRQPYCPFRFDYWSLGVLLFVVVLGKSPFVSARDSDLGFQLYKKEYLKFIEANSLFSQDKSDSIPKNGNFSNPHGNDPNFIYLFWRLCDPNPATRMTLPKLFHNKFFQKLEMCVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSU8</accession>
    <name>A0A1E3NSU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14653</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020292.1">
        <property type="nucleotide sequence ID" value="XM_019160471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSU8"/>
    <dbReference type="STRING" id="763406.A0A1E3NSU8"/>
    <dbReference type="GeneID" id="30177158"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ49179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="213" mass="23577" checksum="4992AD67F3828871" modified="2017-01-18" version="1">MSEDNNAAFISNWRTTRTTNNNDFTNTASSFFTNLGTNISNTFQETVDGAQNILPLNSSDLLGASEEPSWFTLSRFERYLGFVLLLAGSTACFALGFFLFPVLTLKPRKFVMLWTLGSLLFFLSFGVLQGPVSYVKHLSSKERLPFTVVFVTSQVLTIYCAVVLKSTILSLIMGIVEILSILWYTASYFPFGTQTMQMVFGAGARQVSGMVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSW5</accession>
    <name>A0A1E3NSW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019776.1">
        <property type="nucleotide sequence ID" value="XM_019160266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSW5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSW5"/>
    <dbReference type="GeneID" id="30176953"/>
    <dbReference type="OrthoDB" id="870at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="168" mass="18595" checksum="E63F108E59C4D263" modified="2017-01-18" version="1" fragment="single">MGFVDAIFILDQTQFPILHHTLKPEVPSFDYVRSQLLIAKRELLSKCGVDISTEFSTNEIFDSSPSMLNLSTAGSTLHGTTSDYVTLDPVFKLDSDWLVSWCKIDGLYFLTLGTAPQEFLEVVSDDEADEAEDHEEAEEDDGEDYALTKNAGSTADGSNMHSRGEYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSX9</accession>
    <name>A0A1E3NSX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_98374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020370.1">
        <property type="nucleotide sequence ID" value="XM_019165135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSX9"/>
    <dbReference type="GeneID" id="30181822"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="63" mass="7052" checksum="00C534D6D8AA3DB1" modified="2017-01-18" version="1">MELRNSSIRGFFASRIRRIGSRGRAKSVGWLEIAARGGFAVVLKCVTMVKRETETSTHLFVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT75</accession>
    <name>A0A1E3NT75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_57357</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="5">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020414.1">
        <property type="nucleotide sequence ID" value="XM_019163232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT75"/>
    <dbReference type="STRING" id="763406.A0A1E3NT75"/>
    <dbReference type="GeneID" id="30179919"/>
    <dbReference type="OrthoDB" id="9984275at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="186"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="705" mass="80883" checksum="BCFF33B75D76DCAA" modified="2017-01-18" version="1">MNIQSMGSLVLWPRSLIYIRSSRFYSSKVSSDEKLLNSILNEQPENDTTETYNQQPPSTYRKNSLLSVPQKNNAFADSGDKTSKPLPIGPKYWKNKFRKSYRKVEKGLMSPLDEYVSNNIEVIESYNKKYKQSVEERIKVDFKKELHWNDVIPNHSLRKAILRYLASQNNASTNDNRFTSFQKRFFALMSCFASTVAKGPSGSGKSFALLIHALSIRRSHTRGKGINSLIVVKSNSLVRQYEQLTNDILAKMDSKQKVNVKLVSQFLYRGTPLEEMKQDDDLTDYQTPHILVTTPQRLLDVLSSRGMDFVKINSLSYIGVDDFTSMLDENQLLETQKKSPVVNLLDYVLKLQDYRRQHNDPHPQVVLMVEESATESLILQLKEYTKWIDWRKFALIGKFGEEEDIPYYKYIANKSAVSTVLVYPRFSQDGVKGNSTSRRFKVNLYDMKPFDYGSTSSTWLNKLYRESFGNSLVYKKHRNSKWSSIPNVVKKGELEILCSGLGKLLKKKNIIQWIGKNRALVVHPDEVNSKQVVDILSSKTRRKVKVFDIYKDAKIFRTRFSDEDEDDSQLFAINASSLTGLNFPGLSTIFVLGIDSIKSESHLSTIMGRARTSDGLVPEKEYTIFSERTNSSVDEKEDIRARTFIITAMLPDSTIDPYDRNFLERAYLVNGLVNQVDAIGVQENWTDKEKKDYKLAIEGNSANKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT91</accession>
    <name>A0A1E3NT91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_14762</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020405.1">
        <property type="nucleotide sequence ID" value="XM_019160519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT91"/>
    <dbReference type="STRING" id="763406.A0A1E3NT91"/>
    <dbReference type="GeneID" id="30177206"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="6">
        <location>
            <begin position="11"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="678"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="178"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ49292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="959" mass="108077" checksum="0C53990ED6E67E88" modified="2017-01-18" version="1">MADTGSVKESSISVAVRVRPLTPDEKVHLQEEEEEEQQYNATAADNSYEANNSSSSGIANKENLFLRKQRIRPLPKPHAIRKILDVVDDRMLVFDPKPAVEDEFEAGESHGHAYGIGRRRSVSGRGLGNHNSRHKEHKFIFDKLFDETSTQNDIYENTTKPSIEAVLDGYNSTVFAYGATGCGKTYTISGTRDNPGIIFLAVQELFSKIEDQTEKEIEVQVSYLEIYNETIRDLLNIETSPRKLTLLENENKEIIVSNLTKVDASNVEEVMDVIVRGNFNRAVSATEANLTSSRSHAVIQVYITQTPHVTDIYEKSTKSVLSIIDLAGCERASATKNKGARLHEGANINKSLLALGNCINALCDLKRSKHIPYRDSKLTRLLKFSLGGNCKTVMIVCVSPSSRHYDETLNALKFANRAKEIKTKVVRNRTTVMKHVGSYMKIIADQKRKIYELETLMDSTVKREILKYAEQRSNVKTQMGIMIDRLWNNVSKCEQLKSNKIYIVARRKMLLIYTKQLIDFCGSLEKFDIENKTVVLRQSLGKIVSDIKSQIALMEEEYNQRTELDIVLLDTSDSFLGKLRELESWCDEDEVSYAKEVEYLKTDIEKQIFYESSVLFDSWLSETSGVSSLDSIPSILAQFLCMIENIANNIQNVEDARMQCYQLSDSLIQNCMQQLGPEIDTYPPDTTHSNNRKRKNSGNNGIAKTDESGELSFIRQGNRINSINNKLMRINLNSVKDDSKNANIDSLSEVESSLILENDNKPSRISSRVRSSSHSLLLQSESTPQKTGIVDDLSGYISPGVIRKIENNTDDSQQKTTTIDIQSTIPEWDESNSSVIIRKGFDIDEENINPENKDNMLIPHMLGSPFRFMTGISKEATSPSPSHVRKHRNHSGKLTTKKLVASPRITSPHRLVGLDSSSSNMFQFKIRESMGNIKHGEDAGNPNYDRDNDIGMDVEVVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTG5</accession>
    <name>A0A1E3NTG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_70921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020485.1">
        <property type="nucleotide sequence ID" value="XM_019163770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTG5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTG5"/>
    <dbReference type="GeneID" id="30180457"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016278">
        <property type="entry name" value="DUSP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000941">
        <property type="entry name" value="DUSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="109"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="5">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000941-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="43046" checksum="7A0F296C3A70070D" modified="2017-01-18" version="1">MTEDPTLYRTLGPIYISSIQPLLENEDLHQKYKIKTIISVTKSDIPLTYTKEPYTHLQIPIDDLPTEILLPFIPEVNQLILKTIYRPLSKDALDSSLIKENKVQKPSLSGDAVLIHCQSGVSRSVAFACAYLMNRHNLPLSHALHAIRRNNSGKTFQPNDGFMEQLEIYQESGWCVDVSKLRKTSPRWRAYRMEYLITSMIHSGAIADDEKDSNFSKIETSSEKDPNWFGFKCAKCRSLLASSEVYIPHSPPFEKDDKQMYFIKNAFRSKRVVNVQKGHNECTLIFTEPLLWMRDKLVNENSSSADNTDTGRGNEDHEALEGRLDCYKCGAKVGGWNWKGGRCSCGKWMVPAINLLKSKVVQTDRNNKLILPNDDRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTL9</accession>
    <name>A0A1E3NTL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_70989</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020555.1">
        <property type="nucleotide sequence ID" value="XM_019163791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTL9"/>
    <dbReference type="STRING" id="763406.A0A1E3NTL9"/>
    <dbReference type="GeneID" id="30180478"/>
    <dbReference type="OrthoDB" id="47179at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000019">
        <property type="term" value="P:regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009303">
        <property type="term" value="P:rRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03488">
        <property type="entry name" value="Topoisomer_IB_N_htopoI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.41:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.11.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.15.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.41">
        <property type="entry name" value="Yeast DNA topoisomerase - domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013034">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048045">
        <property type="entry name" value="Topoisomer_I_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="10">
        <location>
            <begin position="249"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="21"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="583"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="6">
        <location>
            <position position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="735" mass="85352" checksum="7EF484857047C1F1" modified="2017-01-18" version="1">MSQSIVKKEIVVGDLKAPKRKLKTEAKEPSTKKVKTESTSTKVQKANAKKGTKAVKKETVKVTKKEVEKEVKKDSQNTDDNAEDEDTFKWWENPDDLNGEIKWETLEHNGVMFPPPYEPLPKSVKLYYDGKPVSLPLEAEEVTGFFGALLESEHAKNPVFQKNFFNDFRKVLAETSGCPDADITSFDKCDFSKIYQYYDKKREEKKALPSAAKKKAKEEREKTEEMYKYCIIDGRKEQVGNFHIEPPGLFRGRGAHPKTGALKRRVQPEDITLNLGKESPTPTAPEGHKWGEIRHDNTVGWLAMWKENIMGNIKYVRLSQNSSFKGISDLKKFEKARELKQHVDKIRTDYTKKLKSSMMIDRQIATATYLIDVFALRAGGEKRDDEADTVGCCSLRFEHIFLKPPNTVIFDFLGKDSIRFYQEVQVDSQVFKNMKIFKKEPKKPGDDLFDRLDPSILNKYFQGFMTGLTAKVFRTYNASKTMQDQLDLIPNVGTVKEKVVAFNAANREVAILCNHQRTIPKAHQAGVQKLNEKIEEMIWQKIKLKRIMLQLDKTMKKKDLEYFEEIEDLTKEKESEIVSRWSKNELEKLQKRFDREKEKLKYQKKEMSADEYKQKMAELKEENKKKAEKAKETIKQFKSEAKSQKYEEKSSYTAEKLKLQIDKLEERIKNTSLQLKDKEENSAVSLDTSKMNYIDPRLTVMFSKKYDVPIEKLFTKTLREKFAWAIESAGADWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTV3</accession>
    <name>A0A1E3NTV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Translocon Sec61/SecY plug domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_14916</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins and is essential for cell growth. It interacts with other membrane proteins required for protein translocation. Upon binding to SEC62/63 complex, secretory precursor polypeptides may engage SEC61 to begin membrane penetration event. A cycle of assembly and disassembly of SEC62/63 from SEC61 may govern the activity of the translocase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020583.1">
        <property type="nucleotide sequence ID" value="XM_019160594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTV3"/>
    <dbReference type="GeneID" id="30177281"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070843">
        <property type="term" value="P:misfolded protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000002">
        <property type="entry name" value="Transport Sec61 subunit alpha isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="15">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ49470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="478" mass="52655" checksum="A7743A9BA35F478C" modified="2017-01-18" version="1">MSGFRVLDLVKPFQPYLPEVLAPERKVPFNQKMMWTGVTLLIFLVMSEIPLYGITSSESSDPLFWLRMMLASNRGTLMELGVSPIVTSGMIFQLLQGTQILSMDPESKDDRELLQVAQKLLAILLSFGQATVYVLTGMYGRPSDLGSGVCLLLILQLVFAALMVILLDELLQKGYGLGSGTSLFMSTKICESIVWKCFAPTLVNRGRGSEFEGAILAFIHMLFTRSSTRAALVDSFYRDNAPNMSQVFSTLIVFSLVVYLQCLRVDLPIKSTRQRGPFNVYPIRLFYTSNMPIMLQSALTSNIFIISQMLYSRFPNNLAIKFIGTWEAKQGGGQQLFAISGVSYYMQPPLNLQEALLDPIKTVIYVLFVLGTCAFFSKTWIEVSGTAPRDVAKQFKDQGLVIAGHRETSVYKELKKIIPTAAAFGGATIGALSVVSDMFGCLGSGTSILMAATTIYSYYEMAAKEGGLVKSLGANFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU20</accession>
    <name>A0A1E3NU20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020653.1">
        <property type="nucleotide sequence ID" value="XM_019160616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU20"/>
    <dbReference type="GeneID" id="30177303"/>
    <dbReference type="OrthoDB" id="10438102at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="71" mass="7802" checksum="791D9FBDFAEE95D0" modified="2017-01-18" version="1">MLFDAMRQSLTRQTSISAPTLALGFMGGVVLPILLTNALASKQPNAIYQGKDKVDHVDVADEDYDFNDLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV27</accession>
    <name>A0A1E3NV27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_22290</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020631.1">
        <property type="nucleotide sequence ID" value="XM_019162245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV27"/>
    <dbReference type="STRING" id="763406.A0A1E3NV27"/>
    <dbReference type="GeneID" id="30178932"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="15"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="108" mass="11666" checksum="F7A9DFE8926BA456" modified="2017-01-18" version="1" fragment="single">LGPHQPKNQREIDSRSVYVSNIPFKISPWKLKVLFCQGADGVPGCGSTSVNRVTIVCDRVTGLPKGYAYIEFAHESDAAAAVRFDGLQVDGRVIGVKSKRTNVPGVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND82</accession>
    <name>A0A1E3ND82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_24486</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015195.1">
        <property type="nucleotide sequence ID" value="XM_019162353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND82"/>
    <dbReference type="STRING" id="763406.A0A1E3ND82"/>
    <dbReference type="GeneID" id="30179040"/>
    <dbReference type="OrthoDB" id="292693at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140104">
        <property type="term" value="F:molecular carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="4">
        <location>
            <begin position="11"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ44082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="151" mass="17157" checksum="2A272660FA1D0B3F" modified="2017-01-18" version="1" fragment="single">QKYTISKRFQHPQPLMYALVSRVDLYHEFVPYCTSSFITTRDPATGQPTVAGLRVGFQAFDEEFTCELDCVPSQTVVAKSISHSLFHFLETEWQIVPTDGVNGGADHCVATLNLRYEFKSTLYNHVSSLFAKQVAKLLTQAFERRAYEVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDG8</accession>
    <name>A0A1E3NDG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_18432</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015282.1">
        <property type="nucleotide sequence ID" value="XM_019161997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDG8"/>
    <dbReference type="STRING" id="763406.A0A1E3NDG8"/>
    <dbReference type="GeneID" id="30178684"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="714" mass="82488" checksum="04184C7D5D3C52AF" modified="2017-01-18" version="1">MKAKIRRRTRIDVNSVACNRCKSRKQKCTGNFSTGKPCLGCERANVACEYSKKKTPYYVKSLEKRIKYLEQLLKQEKGGNAEVDNGLAIFDQSYTESPVVEENEEEEEEEEEEDEEEVVLKNAKKIANKGNSNLGADVLTKCAALFPLKDDMEPLYVGSSGLNIASLLQENLKLEIADSLKFSNIQKDIYQSTTLNEYVSRSLDDLLYDGIKLNQFVETYISRIHNKYPFLRRDDIYELHNNRESYLKSGKAHDNKTDMLNSFILLMIYAIGSMMVSRKTNQPGMSNPSYENDDFVFFTSAIKLDLSVVFKHKGILNIHSMLLTVLYQLRLPNGPVIWDMIGFALRLCVSFGFHRKNIDLLHQRPLVYQQRMLTFWSAYSLERTISSSFGRPFTLSDLDIDVDLPINIDESITDGSLLIKEYYLTLKDRKSKNVTSRMQAIHHFKFVKIESEIQNKIYRVDTEFESIPREEINDIILKMKQWASDIPPSSSAEYDYYLYLFNKQIRFLLQPFLCKLKQDDPLFVECIEAASTVCQLSKRMYQNTKTRLSFIALQTVFLSGVTMVYGLLSRKFAWNFSISEGVRCCSDVLFSVAQRAYTCGIFSEIFVKLVAMVQEKDKGNDRDCNDKNTFRVGGRIDLFGQDELRKNVHDTHRKENLEVFNEISLKDENLKEFENLFDFANLDFLGTNKLDALVKRTSTSAQFIEQDTFMGFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDM2</accession>
    <name>A0A1E3NDM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_13769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015340.1">
        <property type="nucleotide sequence ID" value="XM_019160110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDM2"/>
    <dbReference type="GeneID" id="30176797"/>
    <dbReference type="OrthoDB" id="10542150at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="154" mass="17182" checksum="EAF0C28BE9E073CB" modified="2017-01-18" version="1">MVGNDTFTYAPMILDDILDNKASLILLKHENKDDNYLVSMLEANSNGTWALNFMDIAPTIKRDGVDGDQYLSGAGGIVIECQYGGGVGDSYDNDQGWVDAVSKRQGYAHAMSATYNFHHGYIGSSSRLKCATKMYTTDQTMQTWRNWNDVWPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDS6</accession>
    <name>A0A1E3NDS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015392.1">
        <property type="nucleotide sequence ID" value="XM_019162040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDS6"/>
    <dbReference type="GeneID" id="30178727"/>
    <dbReference type="OrthoDB" id="10612015at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1099"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1119" mass="130180" checksum="7A5D3A697AC946D6" modified="2017-01-18" version="1">MVFDRSDLRESIVQLGVRHADNIVDGHLVLPERLNWQRLVDDDFYEAVLEYYNDRPEIRLADQELLVLLGHYKPRELSLVQLLLNFVYRTLPKECQYDPSSYLEERVLVHSPFHVQELSVHFHEGLILGKHKFLSYEELLPFSYQKYLPYVKQLYFDDDVPPICCDTRASLSYKYYLPDRLEDPRYNEFLLVSEGNKFWEAVKSFPCEYNRGTFALCSEIALDIEKGYPHCFTFAQFIKVFPFMRGMKMGSSSTSTFVSRKPSYFNNYIQYAMMVCSSNYSEEQMRRKKKARTDSPKLRNRLKIVSIDCPVKYKITIDFKHKVVLIRSYEKHSKLCHLFQKRKGYTIMRKLMTIMDSNQSSTLYMNTMYDHSKLEYYGRDQICLKNIRVAKNISTNTRSKDLLGNDYMTDFHILNKLIQSKSGNFNFKDRQYSFTFKEEMVVENIFYDRLQGFLIGSKQSLLDLSTQVVFGIDATFNVTQYRSLKLLTVVYQNIQTRKIVVGCVGFLPGSESTGNYSAFISALKNRISLLKGGEEAFPNLKYIVTDDSKAAHNGLKEHFGSNVKVVKCLWHKRINFETHLDKVGVDIMMKVMWDRNPLKARAWLEALKEYYHLALKAAYMKPRSEKFTLLVYKRGMKKNCVKIKSLLLRRSRYLLKVKSFLNYIVSLESDCESWCLSYRCVLPDAYSIDNVPEITDEIRKRGVRALNQLAGEEEMSVLQQHELNLLYGNIYLSLANRNEGRSEEVYCHVKELEHEFNKSYSLFTDHLDNPQFGLLMLTTSNNESIHNALKNHYNMKHCPHLVDLYIKLQNYFYSKITKPNFYSIVVRHDFPFVRDMTLGQQVQFNREMDDFTKEFVAEEIVKAEVHGATVTATLCGCRVRAKNCVPCRHMLQKLHQSCTREARSQLGLDLESLSNSDLSVDIVDQDDKIDILASQLLETALKQNTCYRKIVKRLADIENVVRESKQWEDIDFSANLNLIRRKTSDNSALSYELAKEISKEAKINIDLVAEMFRDLRTESDKKKVEHFFSNINDNIKNLVDGVPSFKDAIRVIDFDLLLQSFGKGHDVEEDHTSGERSSSEDELSVVGTENEDDKEEDPGEGLLKESDDDEGMSLTDLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDZ7</accession>
    <name>A0A1E3NDZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI ethanolamine phosphate transferase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_13524</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI second mannose.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGG/PIGN/PIGO family. PIGG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015429.1">
        <property type="nucleotide sequence ID" value="XM_019160074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDZ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NDZ7"/>
    <dbReference type="GeneID" id="30176761"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051267">
        <property type="term" value="F:CP2 mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16024">
        <property type="entry name" value="GPI_EPT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037674">
        <property type="entry name" value="PIG-G_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039527">
        <property type="entry name" value="PIGG/GPI7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045687">
        <property type="entry name" value="PIGG/GPI7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19316">
        <property type="entry name" value="PIGO_PIGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="461"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 2 C-terminal" evidence="13">
        <location>
            <begin position="399"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ44316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="571" mass="64672" checksum="8108236A8EE4DA71" modified="2017-01-18" version="1">MRYFVLLLGLLLQLTAFLLFCRGFFPTKVLLTPAEVEQFSTPYPNNYHDPEPQFNKVVLMVVDAMRADFLFSNNSRMSFAHSLLNDGYGVGFTAFSNPPTVTLPRLKGITTGSTPNFIDAVLNIADDDTSSSLGDSDSWIRQMFLENWNINMFGDDTWLKLFPNYFAKSEGTASFYVSDFTIVDNNVTRHLDYELSKEGKSYWDCLILHYLGLDHIGHKGGPNSSNMPQKQEEMDSILERIFKEVVLQDPNSLFIVLGDHGMNDVGNHGGSSTAETSAALMMISQKFKGLGLNNDIKAPIALNDDYNYFSKIDQIDLVPTLTELIGLKVPINNLGAFVPQFLELYSNEEKNNILLKNALQLKVLLDKSHGGVHPFPEMDNTELLEFIKNAKEELSRSSSAYDYPFIYAGIGLFLIETLASYMFFAFHFKDHFHIAFSDVVFFVTYSANFIASSMVEEEHHLWWFFTTVYTAFIILKNLRGEKISWLGILAMLVGLRLLKAWNNSGQKHNMKANMKLSTYLSQLPENIGPNIYASLLIISVLPMGIIVNVFGKPESEMVFWFYLQFERSSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE27</accession>
    <name>A0A1E3NE27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015459.1">
        <property type="nucleotide sequence ID" value="XM_019161927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE27"/>
    <dbReference type="GeneID" id="30178614"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133321" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6765" checksum="58E5812088AE6B20" modified="2017-01-18" version="1">MCVVRCVCVCVCVCVCLRCACCSAAPTILSRRTQQQLSMRIRSRGSRHVFQAPQIVPSRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE37</accession>
    <name>A0A1E3NE37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_18297</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015495.1">
        <property type="nucleotide sequence ID" value="XM_019161937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE37"/>
    <dbReference type="STRING" id="763406.A0A1E3NE37"/>
    <dbReference type="GeneID" id="30178624"/>
    <dbReference type="OrthoDB" id="449241at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120513">
        <property type="term" value="C:2-(3-amino-3-carboxypropyl)histidine synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010014">
        <property type="entry name" value="DHP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00272">
        <property type="entry name" value="DPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00408">
        <property type="entry name" value="Diphthamide_biosynthesis_famil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="516"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="619"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="650" mass="72165" checksum="1851FED73D42D9DF" modified="2017-01-18" version="1">MEETLQASAAPALSTAQDEEDAFAKVQGLSEEMLREKRRHLGPRYIGKEKVTSEEIAELIEEYYSLEKLARFLNKRDALTGELKYKVIPLQFPDELVCDSTIVAEILQNKLDEWNKEFHDAKHEAKAADQEEVDFTTSGGAVGMSRDIESVEKTIDSKTCGGNCGSQCKNKDKFHQEVWILADTSYSPCCIDETAAGHVGGDIVVHFGDACLNPVEKLPSCYVFGKPYVDLEAVLSTFRFSFEDKTEKIVLMADTPHSHILPALYERLHAENYENLVVAHVDYEKSGKKSKVIDGYEVLMTTGEGRAQIRSVNRILSGFKQLDINDYLETVDFEDENFEESAKFDGLTQEYSLFHITRPHDARILQLSTKFGALKVLDPKNVQLVYDQFPTMMKRYRNMQVARTASTVGVLVNTLSLANTRVLLNHVTRSIVEAGKKHYMFVVGKPNVAKLANFECVDVWCVLGCGQSGIIIDSFGDYFKPIITPYELKLALMPQVTWTGRWVTDFDTMLAHDGAEDGAAEESLENPSAETGDIHTEGDNDYAPEFDPVSGKLRMSQPLRQLRHLELELELEGNRTSSSAGDNPNESSLVKKLSGTLAVGKTVSTSALGLQGRQWTGLGSDFAQDDGVSSAEGARVEEGRTGLARDYSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE40</accession>
    <name>A0A1E3NE40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Membrane protein TMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74648</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015520.1">
        <property type="nucleotide sequence ID" value="XM_019164833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE40"/>
    <dbReference type="STRING" id="763406.A0A1E3NE40"/>
    <dbReference type="GeneID" id="30181520"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="424"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="58545" checksum="302A5BEA9A256715" modified="2017-01-18" version="1">MGLLLSLPLAPVSIIGSWLSSCLGVSICTCCMNKSVNPLMRTFKSSVATRIMYAVIFMVNSIISWVSLSNSLTKFVEKLTWGMFKFGNMFCKDEQGCIGFTSVQRINFSLGVMHLILAGLLVGVKSTRNPRAVIQNGYWIMKLFVLFTFIIISYLIPDKFFVIWGNYFSIIFSTIFIGIGLILLVDFAHEWAETCIEKIEEGEIYLDNGGLDDEDAGGYVCCNFYGSNLWKQVLVGGTMLMYSGVVLMTVLMYIYFAQSGCSMNKAVITINLLFTLAITGLSITPVVQEYNPNAGLAQSSMCCVYCTYLIFSACLSEPDDKLCNPLIRSNGTRTLTVIIGAVFTFGAVAYTTTRAAANSAFNHKYGFDADDHYTTAMATLNNGAAQNPSYHDDAGAITEAPRSVRRDMRYEAVRQAVNQGSLPESALTDPAYYDPDDDDDDDESPATGEEREYTKYSYVLFHIIFFLATQYIAALLTINVGVSDADNGTFVPVGRTYFNSWLKIVSSWVCYALYGWTLLAPAVFPERFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE54</accession>
    <name>A0A1E3NE54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_13579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015489.1">
        <property type="nucleotide sequence ID" value="XM_019160081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE54"/>
    <dbReference type="STRING" id="763406.A0A1E3NE54"/>
    <dbReference type="GeneID" id="30176768"/>
    <dbReference type="OrthoDB" id="2392202at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="162"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="345" mass="39958" checksum="AA9CE81D015BB02D" modified="2017-01-18" version="1">MPTITSNSIFKTVITSNQSGRFKKLEFLGDAYLEFVITVLLFERFPNLQKKLLVEMMRDLLSNKHLGDLSRQIGLLKGTKSIPQGSKRHADVFEAYFGGIALEAGTTTYFDKRTSNVWEAWNKTTCFMLEKSKDKFMDFADLYASSTNERGCEIEIICGDTIDPLRKITQLRNNRMKDLTDLFNAEPDDYERRFSLERLQVLGNTTLKYFLTKIYSDNLTDYTVKDLANLRDSRMKDSKIIGLTGKYLDQPSYSLLYSLFNKNKKKLGFGPNAAIIKQFLGYLVATHYLNSSDKGMRELFLLGWNECESFVKSIYYDDMLKEARNKHRKFRHSKITDPRVLDSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE90</accession>
    <name>A0A1E3NE90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 10">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_74694</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 10"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015570.1">
        <property type="nucleotide sequence ID" value="XM_019164845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE90"/>
    <dbReference type="STRING" id="763406.A0A1E3NE90"/>
    <dbReference type="GeneID" id="30181532"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="546" mass="60893" checksum="279D2A608E6743F6" modified="2017-01-18" version="1">MSSPTQLPAWKKLEQLYESKGKGFSSRDAFKADTDRFNKFSHTFTNYDGSKILFDYSKNLIDESILSALFELAKEAKVESLRDDMFAGKHINTTEDRAVYHVALRNRANKPMHVDGVNVAPEVDAVLQHMKEFSDSVRSGEWKGYTGKRIENVVNIGIGGSDLGPVMVTEALKPYAHESIKVHFVSNVDGTHIAETLKLCDPETTLFLIASKTFTTAETCANAQSAKNWFLDAVKEKSHIAKHFVALSTNAPEVEKFGIDTKNMFGFENWVGGRYSVWSAIGLSVCIYIGYDNFDKFLKGAQAVDEHFTSTPIADNIPMIAGLLTVWYNNFHKAQTKLVAPFDQYMHRFPAYLQQLSMESNGKSVTKDSHFVNYETGVVLFGEPTTNAQHSFFQLVHQGTKLIPTDFILAAKSHNPIEQNKHQRMLASNYFAQAESLMLGKTEEQVKAEGVTGSLVPHKEFSGNRPTTSILAQMITPASLGALIAYYEHSTFVEGAIWGINSFDQWGVELGKVLAKVILKELENTDPVSSHDSSTRNLINQFKAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NED9</accession>
    <name>A0A1E3NED9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015630.1">
        <property type="nucleotide sequence ID" value="XM_019161988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NED9"/>
    <dbReference type="GeneID" id="30178675"/>
    <dbReference type="OrthoDB" id="3994480at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="415" mass="48085" checksum="C931DBAB26EC71D8" modified="2017-01-18" version="1">MYGIMPLEEYVANYDSILENKPFKYGNMKGKKLDKSKFFNDYSDYEILLRNEVPPKYFKYFDDFSSDSMIDDIEIGRSNNLNLSPVKNHQENGVKDSELLSKKESKLESDSTTLFRNLKKSLVNDENSFSLDLGNLDLPNSINRKRNLPAKSLKTNPTSNHKFHTSDTSEIDGLEDFDALYEKTFKLLNKNSLVDKEGKQLNMMIQSLLETLKTIKDENIQYKANSENMKLIIKDLNSLINSYKTKLRDYFEENRSLKTMIKDERKTARDIKSSNISRNTFEIESDDLQSIDKQIYLLQVKRQRLIERKKMIRETKEVSLDDLSEDIVQKLLKQLQEHSGHTDESMNSSDTGNGNHNDCPFCKKESKQSDNLLTKLLSTSHTSSLDQDEVIDLLASRIKFKLDTSNDGKKLPDIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEL7</accession>
    <name>A0A1E3NEL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_173667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015698.1">
        <property type="nucleotide sequence ID" value="XM_019161572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEL7"/>
    <dbReference type="GeneID" id="30178259"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="94" mass="10688" checksum="1928AD86B237477C" modified="2017-01-18" version="1">MCMMYVSMYVSMYVSMYVQKYLCENGTIQQQRQTNSVSASPSRHTEQQQCHPTANTQLLPPLCHSARPNHLSLPLLSLSLSPLTPEKNEPRTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEQ9</accession>
    <name>A0A1E3NEQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_60308</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Vacuole membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015748.1">
        <property type="nucleotide sequence ID" value="XM_019163426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEQ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NEQ9"/>
    <dbReference type="GeneID" id="30180113"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000001">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="91"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ44635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="160" mass="16350" checksum="26D375F456F5B667" modified="2017-01-18" version="1">MSDLCPVYAPFFGSIGCAAAIIFTCFGASYGTAKSGVGICATCVLRPDLLIKNTVPVIMAGIIAIYGLVVSVLISSSLKQQQALYTGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVALLMNSRATQDVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEX6</accession>
    <name>A0A1E3NEX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis protein NSA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the pre-66S ribosomal particle.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015818.1">
        <property type="nucleotide sequence ID" value="XM_019164763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEX6"/>
    <dbReference type="STRING" id="763406.A0A1E3NEX6"/>
    <dbReference type="GeneID" id="30181450"/>
    <dbReference type="OrthoDB" id="18388at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22858">
        <property type="entry name" value="Nsa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037379">
        <property type="entry name" value="WDR74/Nsa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038">
        <property type="entry name" value="NOP SEVEN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="381"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="48426" checksum="43D462138BC71A15" modified="2017-01-18" version="1">MKLVVACEDTGAIKVVTALHGTDTSKPADDASPAQAVSITTHAEGNTRRSKVLQLVHSKHTGNIIVSRLNGAVEVYDTSKLEPAAEPLKDGAEKNDNSSGTIDLLQLVQENKSLIPPFENKDSEIFIDLLLDDSGRVIVATNKGSVFIWASEDKISNEPLKFTLPLDANESIESLQIHPGKENVEYIAYGGKETDLRVVKLPSLEKTKKRKNQPEVIFKAKNLSNSRLELRVPIHIKKILFDQTSSPDHLKIYTFTQWGDMRFYDSALGRKPRSSVLVLPKKASIINAIWLDSNLVLCDNTGVVVKVDPSTGSLMSKFKGHIGTTHALDNFQNSILATTGSDRYVRAYDNETRECIIKVFVGTQSNAIAIIEDIESWKGKRQHKHTSGVSGEERMSGAGNGEKREEEEESSDEEELWNKLESNITQRRKRRRLALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF66</accession>
    <name>A0A1E3NF66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Regulatory protein MIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_22813</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in glucose repression of glucose metabolism genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the creA/MIG C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015894.1">
        <property type="nucleotide sequence ID" value="XM_019162279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF66"/>
    <dbReference type="STRING" id="763406.A0A1E3NF66"/>
    <dbReference type="GeneID" id="30178966"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000089">
        <property type="entry name" value="DNA-binding protein creA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000152">
        <property type="entry name" value="DNA-binding protein creA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ44781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="55" mass="6705" checksum="F74EDF1F6F0B9691" modified="2017-01-18" version="1" fragment="single">RPYKCPLCEKSFNRLEHQTRHIRTHTGEKPHQCNFPGCFKRFSRSDELTRHSRIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFE1</accession>
    <name>A0A1E3NFE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015970.1">
        <property type="nucleotide sequence ID" value="XM_019161582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFE1"/>
    <dbReference type="STRING" id="763406.A0A1E3NFE1"/>
    <dbReference type="GeneID" id="30178269"/>
    <dbReference type="OrthoDB" id="3996840at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013239">
        <property type="entry name" value="RNA_polI_Rpa14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08203">
        <property type="entry name" value="RNA_polI_A14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="124" mass="13812" checksum="61CD1E2C9B7DAC04" modified="2017-01-18" version="1">MSRQGQRFRAIKDDTNNPTLFKITQSQTFLEDETVLSHVDEFINNHPLNSITANTGDLASVSSSTYTGINPTVLSQLNRLQRNLRGLPPLFDDENPIATTSKPQAELSEVQVGPKVNKKIVFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFE7</accession>
    <name>A0A1E3NFE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_18196</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015929.1">
        <property type="nucleotide sequence ID" value="XM_019161910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NFE7"/>
    <dbReference type="GeneID" id="30178597"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048203">
        <property type="term" value="P:vesicle targeting, trans-Golgi to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000007">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="10">
        <location>
            <begin position="3"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="158" mass="18330" checksum="D19220A4A4FA225E" modified="2017-01-18" version="1">MGINFLILLSKQGKVRLNKWYQAVAEKEKQQIIRDITAIIPLRRAKMCNVIEYKDSKIVYKRYASLFFITSIANDDNELITLELIQRYVEIMDKAYGNVCELDIVFNFQLAYYILDELVIDGNIQESSSTEILRKLKTCDDIEEADLLAGASSRKYIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFR0</accession>
    <name>A0A1E3NFR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 13">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2 13">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_17479</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2 13"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016084.1">
        <property type="nucleotide sequence ID" value="XM_019161619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFR0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFR0"/>
    <dbReference type="GeneID" id="30178306"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007231">
        <property type="term" value="P:osmosensory signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016241">
        <property type="term" value="P:regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033262">
        <property type="term" value="P:regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051403">
        <property type="term" value="P:stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07856">
        <property type="entry name" value="STKc_Sty1_Hog1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000049">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000050">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038783">
        <property type="entry name" value="MAPK_Sty1/HOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="439" mass="49814" checksum="1069D419A7E77872" modified="2017-01-18" version="1">MSASQDFVRSNIFGTVFETTSRYSDLNPIGMGAFGLVCSAKDKLTNQNVAIKKVMKPFSTPILAKRTYRELKLLNHLRHENLISLEDIFLSPLEDIYFVTDLQGTDLHRLLTSRPLEKQFVQYFLYQILRGLKYVHSAGVIHRDLKPSNILVNENCDLKICDFGLARVQDPQMTGYVSTRYYRAPEIMLTWQKYDTEVDIWSVGCIFAEMIEGKPLFPGKDHVHQFSIITELLGSPPPGVIDTICSENTLKFVQSLPHRDPIPLTERFKDVEPEAVDLLSRMLVFDPRRRITAAEALAHPYLAPYHDPTDEPVAEEKFDWSFNNADLPVENWKIMMYSEILDFHEIEGAGALDNFNLQQYESQMLEQQQTQGQNNIAMDQAQAQAQAQAQAQAQAQAQAQVQAQVQAQVQAQVHAQQAQGQSQVPAGQMSHGQNLEFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFR6</accession>
    <name>A0A1E3NFR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminopeptidase P N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73779</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016093.1">
        <property type="nucleotide sequence ID" value="XM_019164584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFR6"/>
    <dbReference type="STRING" id="763406.A0A1E3NFR6"/>
    <dbReference type="GeneID" id="30181271"/>
    <dbReference type="OrthoDB" id="4215474at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF4">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="7">
        <location>
            <begin position="36"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="500" mass="55938" checksum="1892C254D9BD3322" modified="2017-01-18" version="1">MLKIARRCYATTFRAGQPIFETRPHLIKAGDLTPGISAVEYYQRRLKLAEKLPKGSLAVLPGSSVKYASGSVFYQFQQNTDLYYLTGWNEPDSVAIIERPEDSDDPHDHIFHMAVPETDAAAEQWEGERTGVEGVQEIFNADVGVSVQHFPAHLDKLLRKYKDIYYDIGGASGASGKSVNFFERMFPSTVLNDNNRLTNRANIEETLRKHSKLGFSIQPLSHLITDLRVIKSNAELKIMRLAGKISGRAYNQAYAQRFKSEKGLHSFLEYRFISGGCDKSAYFPVVAGGSHALCIHYVRNDDLLKDGEMVLVDAAGNLGGYCADISRTWPVNGTFTPAQAELYESLLNVQRRIIRECKVSRGHSLNDLHNLSVDLLTLELRNAGFQDVQSWEVAKYLYPHYIGHNLGLDVHDCPLYSRSAKLRKGQVVTVEPGVYIPDDERWPLEYRGMGIRIEDDVAVGDDNNIVLTAEAAKEIIDIESIASSGVTTKIEDEVVDIYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFS8</accession>
    <name>A0A1E3NFS8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamyl-tRNA(Gln) amidotransferase subunit F, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_73791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016104.1">
        <property type="nucleotide sequence ID" value="XM_019164588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFS8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFS8"/>
    <dbReference type="GeneID" id="30181275"/>
    <dbReference type="OrthoDB" id="4053592at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20977">
        <property type="entry name" value="GatF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19034" checksum="90E060069A7D4B72" modified="2017-01-18" version="1">MVRRGPKGFLQKIRGISTGSVVGKRVESSEELDAIFAKPSWSTSDLLDKEAEPVEITEDMLNGLLELSGLSKSLSKEEKTTIVNSLTEQLSFIRKLHNVELKRGAQNSARLVDDQSVQPLTFENLMERISTTQGMMSKGEVENSWNPISLASEHQNRFFLVKEGLLKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFV8</accession>
    <name>A0A1E3NFV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015561.1">
        <property type="nucleotide sequence ID" value="XM_019161961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFV8"/>
    <dbReference type="GeneID" id="30178648"/>
    <dbReference type="OrthoDB" id="5587367at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="104" mass="11450" checksum="22BC1FA610B9FB18" modified="2017-01-18" version="1">MHCLADVALTPVGPAEYGFEEALASCVPILEKSGLTYVVHDGGFTIDADWQAATDLIGLLHQRLHDAGFERVHSDIRIGTRTDKAQTMQDKVDTVERQLAFPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG22</accession>
    <name>A0A1E3NG22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Potassium transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_12828</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016203.1">
        <property type="nucleotide sequence ID" value="XM_019159971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG22"/>
    <dbReference type="STRING" id="763406.A0A1E3NG22"/>
    <dbReference type="GeneID" id="30176658"/>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="629" mass="72189" checksum="991F748A9C524D57" modified="2017-01-18" version="1">MNLYNSSQDLALRLKNIDNYSASVDQNPKKQDIRFADLPKPRKRASHYTDEASDRIFNFDSDIEGETDEEEDEANLLRTVSVQNSRGRKRGRFNIFKRKLELKKMLLNQDNELNGSINSSIVTTYGKTNDDKSSIFRGDYTYGSIDAEDTKFAEDNCSLRRSHTSVMSDTIRKIRNSVFKRSLTSYSNRTFTLMNLNELNDEELLDFATRNHVKPFVWGFHGRNFNHIPFTKQQKEELDGIEYQAMKLLGRVIFGYYFGILVISTLCLFWLVRQKKGYEEILVSDGCLNSTWWAFFTALSAFSNEGLTLNISSFKVFDQEISLLSIITIIVLAGNTVFPINLRILVWTLRLMTRPSTMAYDSLTFLLEHPRRCFTLIFPSGATWWLLFVLICMNMLDWALFAVLDFGRESLSYLPEWYKGFDGLFQVICTRTTGFNVFQLADFSHAFQLSYVVMMYLSALPLAISIRSTNVYEDQSLGIYKLDEETAYTKPKLEYIVAHIRKQLSDDIWFIFCAVFIICVVEGQHLDAGDIDFDIFRVIFEVVSAYGTVGLTLGYPGSFASFCGEFHAISKLVIMVAMLRGRHRDLPNAIDRSILLPGKKLNSIDDKENEIHLQRVGTDISGELTGGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG57</accession>
    <name>A0A1E3NG57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">STE12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_18699</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STE12 transcription factor family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016240.1">
        <property type="nucleotide sequence ID" value="XM_019162082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG57"/>
    <dbReference type="STRING" id="763406.A0A1E3NG57"/>
    <dbReference type="GeneID" id="30178769"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990526">
        <property type="term" value="C:Ste12p-Dig1p-Dig2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990527">
        <property type="term" value="C:Tec1p-Ste12p-Dig1p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000220">
        <property type="term" value="P:regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003120">
        <property type="entry name" value="Ste12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052127">
        <property type="entry name" value="STE12_transcription_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427:SF1">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02200">
        <property type="entry name" value="STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00424">
        <property type="entry name" value="STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="14230" checksum="CCDB1BD4E0FFB68E" modified="2017-01-18" version="1" fragment="single">FLATAPVNWHENQVIRRYFLNKEEGFVSCVYWNNLYFITGTDIVRCIAYKMAHIGRQIVDRKKFEEGIFSDLRALKCGTHAVLENSRSQFLKFLHRNQCLRTQKKQKVFFWFSVPHNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG71</accession>
    <name>A0A1E3NG71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_60493</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015456.1">
        <property type="nucleotide sequence ID" value="XM_019163439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG71"/>
    <dbReference type="STRING" id="763406.A0A1E3NG71"/>
    <dbReference type="GeneID" id="30180126"/>
    <dbReference type="OrthoDB" id="546861at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="7">
        <location>
            <begin position="137"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="229" mass="25677" checksum="19DA659085F6E6FD" modified="2017-01-18" version="1">MDPFNSLYASTTPLIDQLSSTSPQSLDFLTLQNEIVSCIHDLEESVKVVESNNGNILDNPFGEISQDEIAERKMQIQGLHESLNKILAEKNVSTLKGGYGANPFADNMKIHTTTDSYRSEDQDPPQLDSATREYHQQLLQQQDDLISNELSRSINNLHQQAVTIGDELDYQQDLLDQVENNMERLNFKIVNTGMKRINKFLATNEMGGNCCIAVLIVVLIIILVLLIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGI1</accession>
    <name>A0A1E3NGI1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016360.1">
        <property type="nucleotide sequence ID" value="XM_019164648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGI1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGI1"/>
    <dbReference type="GeneID" id="30181335"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035543">
        <property type="term" value="P:positive regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="669" mass="75056" checksum="52B9F3DC9B0DD9CA" modified="2017-01-18" version="1">MSLISSTQTLRVRQITNLKKMLHLNIDVDNELVTSTDETDLIWKVLILDNKSTAIVSSVLRVNDLLEFGITVHALINQRRAQLQDVPVIYFVEPTTENVATIISDLENDQYSQFYINFTSSLSRSLLEEFAKRVALTGKADRIKQVFDQYLDFVVTEPNLYSLDMKNVYFQFNNPQTTESIINEKVETIASGIFSSILTMGSVPIIRANKGGPAEFIAQRLDEKLRDHVINTRRLSLNNPNSSSGASSNISSDKAVLILLDRNVNLTSMFAHSWIYQCMVADVFKLERNTIKIEKNSGSNGSEKVEVKKFDIEPKDFFWNQNASLPFPDAVENVEKELNKYTSDAKQITAKTGYSSIQDIDVNDQADTQHIQEAIKSLPELSHRKNIIDMHMTVLSDLIKELDSKNLDSFFEIEQNLNDPKVQRQLLDILSTNSKADNSMDKLRTYIILFLSSDLPKSFCDECESKLAQLNIDLSPLNYIKKVKEFSKLTEMSLSTKTNPGNESSSYLANNGALFSNLSSKLINLADGSSKISEGFGSLVSGIKKLLPEKTHLAVTNIAEAIMNPTQANANSLKLTDDYLYYDPNITRGSHSKQPKRKTYNEGMVFVVGGGNYFEYSNLQDWCSDWNVKQGTSNGAVNGGSPTKMVCYGSTEIITATEFLEECRELAMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGS2</accession>
    <name>A0A1E3NGS2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_13223</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015656.1">
        <property type="nucleotide sequence ID" value="XM_019160030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGS2"/>
    <dbReference type="STRING" id="763406.A0A1E3NGS2"/>
    <dbReference type="GeneID" id="30176717"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="537"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="781" mass="87121" checksum="034A05159C9AD251" modified="2017-01-18" version="1">MSGTTLARSNIYPTDEDLFIAYSESLPIISSTPSILKSSNSLSPEERDSHPAPPAFIVHSDISKTHTYADDQIIPWRKLHAKQRIEESALQSSLTSSNVMKFLVKYNRWPIIITSSIIISFLAIFLDWSSTWLNDIKYGYCETNIFDVRESCGAEKWHFYGSGRSSILLGILKLMLITLVSIVLALIGLFISKTSFWISKSGISELKMIILGHVNNEFLQPSIIVKKFIALIFICSCGGLLVGYEGPLIHISCGVISFVIEKFSSSSIFFQSLNNEAIKREIISIGFVIGISLAFGAPIGGLLFSIENLRLGTRVNTLAWNGFVCSSIATFILVEIHPFKRISINEAFKVDIANGWILFETLPYVFVGLVCGLLSLLFCKLHMKLIDIRAKFKTYDAMPKIIYKLINNSFAEISLLVLITHLLTYPLEFSPLTLNNILSTLFYDCDENLPNGHNLSIFQYQNKLFALAYFFVVLFFLSNYSYCLDIPGGMLLPSLTIGATLGRIIGELVQIIQIKSGSNIFLQCYEENKKCVSPGSYAIVGAASFFAGFTNTSVAAVVIVFELTGAVTYLIPLMLGVVISKTVVDIFGYKGFDELWLMKISKTYLSPELTETMSLSQFSEVPISDAIGDYDPHILYVDDVLLTVKELSEKLESVYDENNGGNISNDGFVLLTNRFNCNLVGWINFNDLSQILKSSNFESERLVSFMTTSNEMKNDNIIELDSSIVPSNEIIKVNAEYSLLSAYELMNRMLITNIFVCSDQRDGSGFKGILRMADLAKLIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGT5</accession>
    <name>A0A1E3NGT5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">General negative regulator of transcription subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_74098</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016447.1">
        <property type="nucleotide sequence ID" value="XM_019164678.1"/>
    </dbReference>
    <dbReference type="SMR" id="A0A1E3NGT5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGT5"/>
    <dbReference type="GeneID" id="30181365"/>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032195">
        <property type="entry name" value="CNOT1_HEAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16419">
        <property type="entry name" value="CNOT1_HEAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2370"/>
            <end position="2391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2630"/>
            <end position="2649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2655"/>
            <end position="2676"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat 1" evidence="18">
        <location>
            <begin position="305"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="17">
        <location>
            <begin position="551"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="16">
        <location>
            <begin position="737"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="15">
        <location>
            <begin position="945"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="14">
        <location>
            <begin position="1299"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="19">
        <location>
            <begin position="1593"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="13">
        <location>
            <begin position="1946"/>
            <end position="2304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1494"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ45334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2710" mass="307488" checksum="4D8C979B6EF66CB2" modified="2017-01-18" version="1">MAPDDDTKSTPLPPSRYYFVLTFVKISNNRKATSPDPISINNSVSTSTTTTTTSPLFKTSPLSKDLTRAILSQLSILITELNKDNCIQIIHQIHTILDKHQEIFPIFLRRLINQISTEIVEDKSIDYQHSYTHKVLKHELNYLTLVELEKVSFISQALLVPESQFLIFTLDKFLSFFNLTEFQSLLISLSIEQFILNNHLEEKYSSLLEDLPTIYVKLSSGVVQWLDLLESSKSSTRDNLATFLRLFLSSKVFSAPHKFIVLSHISESFKKTGTVPEKLPYQTKFEKVNTVINQFLDTVKKMNYKDLILEFCNTKSSPDKLLSDLLAIQPESNLTDSVVILLSETLYPGSQRSTIDKSNEPSTGSLLTATSLPEATARGARMLQVINRVKSQVDWDASFDKILQMFDSTRYTITNISLSQFFASIQTDNLIDNFLSKLCKFSNVQLLTQVFVQLNNLDPNAGAIDLFSLELTPILQTESNVRHLLLYYKYIVMLEIKTVALLSFSRLNDNIVSQIFNKDLRAASEYMILACLHLLHDNPNGEENEIIINIMQNFFINLLDINSPYLSAIFALFEEYNAKEFGKLLLKYLELRKNQESIHKIASLTASFKDDTVIISILQNCSDFQEGFTISVIFSQYGWKNFKAFIDKQLELNANLVSATIVDYLEKQARVEYENAQQGKRALKSLNLETVYFLLTTLSSEQLPKELFEKLRNLQTLCLQAYPRLINFGQGHDAAILMNSSTNTFSVGVEKEMKLYYQKMYKKEIEIKDVVHMLQRLKNSDNPHDQDVFACMIHSLLDEYRFFPEYPVDALATTSVLFGNTIVSKLLEGSALSIALRYILESAREPAQSKMFKFAIQALYSFMKRLPEFPKFCSMLCEIPALKSQPELLDVCQNIASGKIVPAASDSPQVSDKNLKNNALDDDPVLVSKYVSIAIPELTYGIVTQELPPNEVSDRLLFMVNNASVTNVESRVNEMKGLLQPNHYKWFARYLVNQRAKLELNNQELYAAIVEKLNAHDFYTYVLIVTIRQIVSLLNKTYSDPSSSVSDLTSTEKTHLKNLGAWLGRISLSNDHPILRKDIAFKSLLVEGYDQRKLEYIAPFVCKVLDHTKNSTVFKYPNPWLLGILQVLKELYDVADLKLTLKFEIEVLCNDLNIKLDDIEASTVIRAHQPGALENLVNSQKILVNMAKMALNEKKGIPPITPTVEMANLGNALLQRQQQQQQSALQQVQQQRLLNRNGLQNSPAIANIAPLAQQAAILNAQQQQQQQQQQQQQQQQQQQQQSSQAFENLIGNTIFITHPALKRILQLSITKAVKDLLPPLVHRTNTVCLVTTKTLIQKDFAFEVDDFKIRKAYINTVRYFAEYLILASSTDLIRDTIQTNVQQYLQTYMNSLNTPVDTSFVEQIPVAVNDNLQLALSIIQKAAVEKAVHDLDDAMLPAIALRRQFKSTSSNQLFCDTQNASKYAMTLPEPLGIKPGGISHEQYRIYDEFGKARGAGVSGQPEGLPASNFNNENPLAGGAAPDVKQRLLQQQLLLQQQQQAQQAQQAQAQQAKLPAQVPGTILEQSMNFINQHLETIIKTINSIDNKNIRLSDNLPETEPIKSLLVEVIQALTRLGQQELYMKYAQIALNTLFGVAGPSQLLIDSFVFMLGNICDVSSFVVRFITAWLFNSDDERKLDVKVLTTFVLEGFISLNDLSYSLCKRIEANKDQKVISFTCDLIEELVFGEQPIALRSDFVRVIELLGSADETIQSMSKVKELFEKLASPEGDALSRLKNGILKTSDVKDYMAYMFSEWVKLYKFSSGSDLQIAFIEQLVGSGIINKPELFTQFFTVATEIAVVSFVKENDNFKKSSIETYTAVDSLAKLVVTLMMIQSDNAGDNQVKIAFLSKFCSAFLLAFANDHETNKASFNERPYFRFFSTLICELSTLKQNKYAPFASSVGEVESQNRLFVQIYQLLADVFLCLQPAAFPGFTYAWICLISHRMFMPTMLELDDGENSCSEKFSLLLVALLRFQSGYVKGKNIPESITVIYKGTLRIMLVLLHDFPEFLTQWFNPLICATSLTFIQLRNIILSAVPFDMVIPDPFQQGLKVDRLPEISTAPIVSSDPSQLLLKKNIKKTVDNYLRIPSNSLLRQILLVLELSEPVSEGGIGFRKVKYDIPLINSLVLYTGISYVDERPKNSLNFNSKSSQVSLLTSLMQEGDVELQFLLLQAISNNLRYPNAHTHWFSCVVLNFFSSKTLWAEKKQDVQQLITRVLLERIICNKPHPWGLLVTFIELLKNKDYEFSSLSFTKLSPEIEKLLTTLITHLDSTKMFSRIRNRFKRASDDDDVDDDSEFRERVARKIHSRRPPNTAFRQQRLKSWQPVLTAKVVLPLLLVVAVIFLPIGIGIFLTTYRVQLVQINYSNCDTLASSDLSDIASRNYHYHFKGTKKSSAQWSYDGDSKVCTVQFEIPEDIKGNLFMYYKLTNFYQNHRKYVESYDWNQLRGEAVSWDDLSDKCEPMRYRDNKIIYPAGLVANSLFNDTFSDLVNVDDPSRNYSFSHQGIAWKSDLGIYKTSQYNVSEIVPPINWLERYPSGYTADDLAEISSNEHLMNWMKTAALPSFMKLYGQNKEKDNVLHAGKYQVKIGMRYPVTIFGGTKTLIVTTSSVVGGRHMGLGICYLVAGGLSVLFMIVFLVKQLLTRNRREHAFLDSLNDGHPLNSESGGLRQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGX1</accession>
    <name>A0A1E3NGX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_79119</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFG1/PHD1/stuA family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016518.1">
        <property type="nucleotide sequence ID" value="XM_019164966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGX1"/>
    <dbReference type="STRING" id="763406.A0A1E3NGX1"/>
    <dbReference type="GeneID" id="30181653"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000003">
        <property type="entry name" value="Ascospore maturation 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029790">
        <property type="entry name" value="EFG1/Phd1/StuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792:SF1">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="6">
        <location>
            <begin position="344"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="482"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="629"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="137"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="506"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="546"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="662"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="685" mass="73202" checksum="1E4A371DEBE91608" modified="2017-01-18" version="1">MYNNQGYNGSYQNRYYSSQLGQPQTAAAAAAAAAAESQAPSGQQAHLQQQQANPDTQQVQQGALESNQQQQQQQQPPVQGQQQSYYPSNASYSSYSGVPQNYTNPVSYTGAMYPSSYIQDSTTPQAATANPLSASSQPQSLGPQDQAQQQQQQQPQQQQQQQQPQQSQSQQQQANSNPSGQAQSNYSSAYGYYSQPQNMLDPMSSYASQMYQSYNGYPNSKLGYRTYPSGSPLATYPIYPDYNNQLNAATPQISNLQKHYNTLNIPNLNMNVNPGQPNPQSQGQGQQQPQQQVPAQVQQSQSQNVRGLPSHNGSTSTVVSNANSSGFQLSPNAGQIQPPGSRPKITTTMWEDEKTLCYQVEANGVSVVRRADNNMINGTKLLNVAKMTRGRRDGILKAEKTRYVVKIGSMHLKGVWIPFERALIMAQREGIADSLYPLFVKDIQKIIQQGTPTVKMGQYALNPKPSHGVANDAALYAQMNPQAKTTKGPNDGSKVLDRYDAHTDGRGNAAYGAADGADSVDPTGSAKLSGSNHSESHVYGGPGSVNNPASSTNPSHSNSNNNNATTSAASATTATDATNPSSNATTAATSNHAPGSVAPMMYPSYYNSAHQSQPSQSQLNPSQSYYYGGYYAQNSQPASSSAIPSQKYAQGLSADVAGESVNPTANNSGSGSSSRPGNPNADNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH29</accession>
    <name>A0A1E3NH29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016550.1">
        <property type="nucleotide sequence ID" value="XM_019164705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH29"/>
    <dbReference type="STRING" id="763406.A0A1E3NH29"/>
    <dbReference type="GeneID" id="30181392"/>
    <dbReference type="OrthoDB" id="10014216at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110130">
        <property type="term" value="F:ribitol-5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000121">
        <property type="term" value="F:sn-glycerol 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043136">
        <property type="term" value="F:sn-glycerol 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050286">
        <property type="term" value="F:sorbitol-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052646">
        <property type="term" value="P:alditol phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050849">
        <property type="entry name" value="HAD-like_hydrolase_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006384">
        <property type="entry name" value="HAD_hydro_PyrdxlP_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01489">
        <property type="entry name" value="DKMTPPase-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181:SF2">
        <property type="entry name" value="PHOSPHORIC MONOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181">
        <property type="entry name" value="UPF0655 PROTEIN YCR015C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12710">
        <property type="entry name" value="HAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26039" checksum="57ABBAA7139DD6F3" modified="2017-01-18" version="1">MTPPRAIVFTDWDGTVTLQDSNDLLTDNLGMGHPQRMALNDRILDGSLSFRDAFALMLGSVAANGHSLPHCIEYLLQNVKLDPGFSDTIEWCVANDIPLIVVSSGMDVIIETLLKKLLRPQLHPFIQVYSNHVESPNSAGSWNIVYRDDSSFGHDKNQSIQHAAALYYPDPATRGTLFYCGDGVSDVSAARSCDLLFAKQGHDLVDICKRDGINYLEFASFHDILTQIQQELKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH79</accession>
    <name>A0A1E3NH79_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_22326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015816.1">
        <property type="nucleotide sequence ID" value="XM_019162248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH79"/>
    <dbReference type="STRING" id="763406.A0A1E3NH79"/>
    <dbReference type="GeneID" id="30178935"/>
    <dbReference type="OrthoDB" id="3990500at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="141" mass="15050" checksum="7AB2D6CD52FD0BF5" modified="2017-01-18" version="1" fragment="single">LLGVLGVSSLYLGAHKPLLNDAGYANGPVSITTPGSPNIYYDNKKPVYDGAFGGRLNYRQVALGSMTGLVIGYAVSRLSTVLFVFSIFAYLIGIYLRKQGVVVVDTKGLVKGAVNSVSWDELLFDQTSFSVPFLSSFCIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHB8</accession>
    <name>A0A1E3NHB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_59206</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016640.1">
        <property type="nucleotide sequence ID" value="XM_019163350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHB8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHB8"/>
    <dbReference type="GeneID" id="30180037"/>
    <dbReference type="OrthoDB" id="2099276at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="9">
        <location>
            <begin position="161"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="378" mass="43459" checksum="37907FB7AFB33BB2" modified="2017-01-18" version="1">MSRSRHFTRAATLRQTGSSVTIKHEDLGTFDSEIKKFDKERQNQPPTIARKKRNIKHIKLEYEDQDEEAAGAESVDISENFFPLYNKIKEMRELIVTPVDLIGCVKIPTLLKLITNHYKETGVIRHIEIAEISGLQDELKLIEKHGTDRFSRYQLLVTLLFSSQTKDEINFQVMQALHAHYLQKGYKEGLCIESINDTDEKELDKMIFQIGFHSRKALYLKETTASLQNGDIPDTIEALVKLKGIGYKMGHLILQGAWNKVVGISVDTHMVRLCNMFGWTPKNEKNPDNIRKILEQMLVNHKEMWNEINPVLVGFGQTVCTPVGRRCDLCLLSTIDKKNDVVCPAIDKKLLLRVQRGVEKDDRKIRGDIERLVEYKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHW8</accession>
    <name>A0A1E3NHW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_73936</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016271.1">
        <property type="nucleotide sequence ID" value="XM_019164627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHW8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHW8"/>
    <dbReference type="GeneID" id="30181314"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009027317" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="22"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="586"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="626" mass="71200" checksum="54E7DCCE90366E6E" modified="2017-01-18" version="1">MAGYTKLFCALTVFLSASANGFYLPGVAPTSYNEGDAVQLSVNHITPSLMKADEDSATFLYSFDYYFERFHFCVPEEGKQKQSESLGSILFGDRIFNSPFELKLLQNTTCNKLCTVTYPAEDASFVNKYIRAGFQFNMMIDGLPVAKELMDSQTDDIFYSSGFNIGYDDKENLPHLYNHYDFFIEYHPRSDGSYRVVGATVDATSIQYIDPDEINCNAEVFEPVRLKEDEQTDVTFSYSVYWVPSDTAWATRWDKYLHVYDPKIQWFALLNFSLIVVFLSIIMSHILLKTLRNDIKKYNEVNLDDDMIDEMGWKLIYGDVFRPPKNPMLLSVLVGSGVQILLMALTSCFLAAFGLLSPSNRGSLATVMFVLYSLYGLIGSYYSAYVYKFFQGQDWKINMFLTPVLVPGTIFSIFILINFFLISVHSSGAVPAGTMLAVFLIWFAVSIPYSCIGSLLALKKKAIELPVKVNQIARQIPPQPWYMRTRYLALIAGIFPFGAIAIEMYFIYNSIWFNRIFYMFGFLFFCFLLMLVTCMLVTLSLIYFSLCNENYNWQWKSFFVSSGIAIYVFLHAIILSRLKLGGLTSIVLYVGYSMLIAATVGIMCGTVGFLSSMYLTLNIYGQIKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHX0</accession>
    <name>A0A1E3NHX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAM192A/Fyv6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_73256</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016858.1">
        <property type="nucleotide sequence ID" value="XM_019164426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHX0"/>
    <dbReference type="GeneID" id="30181113"/>
    <dbReference type="OrthoDB" id="10491240at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019331">
        <property type="entry name" value="FAM192A/Fyv6_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10187">
        <property type="entry name" value="FAM192A_Fyv6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM192A/Fyv6 N-terminal" evidence="4">
        <location>
            <begin position="46"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="183" mass="20730" checksum="1DD939305A8CFE12" modified="2017-01-18" version="1">MSRFVDDEEAEIQVSSGLSGVEQSVKRGAAENDGTPAPDAAAGVPEEADEEATKDAGAHHYRKRKSLQEQLLNNRMYKYRERRKKLEKQNSSFKMRKDTREFYGKLSAEKQAKKQAERDTYMRELALFRRQREKQKRAEEEAEGETEEEQDLEEAAEPAAEPAAEPEEAPVAGLVAYSDSDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI31</accession>
    <name>A0A1E3NI31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_12243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016902.1">
        <property type="nucleotide sequence ID" value="XM_019159901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI31"/>
    <dbReference type="GeneID" id="30176588"/>
    <dbReference type="OrthoDB" id="3997806at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="335" mass="38040" checksum="6DFD5395D98B4552" modified="2017-01-18" version="1">MSHPATPATPVANSPSSLSDVPYSHTPTQLSTPSMSYSSVGDNKLPFQTTYEEYEKFIHEIPSIASTINRNDQVPQQTGNISASPQTPMTSLSSITVPNPQAFASHLDAVTHLQTRLNYRFLELEAKLRFLRLLTAGTELTSSELVSLDADSEQLSIKIDNLEQQVQEMKKIGRILRKKIGKCFHQLEIRRCIEGKPILEEEINAMVEKTLEYKTFFEENGMEYGDNKSSEPISWAKQFIEENVFGVDLLETNLNRITSELDELEKTLLDLESTSQSITKQEEELDEEIGTLEREKMELEKQLSVLSTHKKTPQEQELIKQHRVLTQLVGIWESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI35</accession>
    <name>A0A1E3NI35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_60022</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016341.1">
        <property type="nucleotide sequence ID" value="XM_019163409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI35"/>
    <dbReference type="STRING" id="763406.A0A1E3NI35"/>
    <dbReference type="GeneID" id="30180096"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF103">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="56693" checksum="0460C96DA3292460" modified="2017-01-18" version="1">MISVSSSAKSTEKNSANEKGLLGIISNEKGLLVADEYDDVTYTEEEEKAVVKKIDRIVLPLLCFVFFSQYLDKQSLSYCVVFGLKKDLHLTGNQYSWLTSGFYLAQLASQFVYIYLLSRFPIKLVTGISIIIWAAVCMCLAAPTNFAGMVTVRCFLGFFEGCVSPAFVMVTSNYYKKSEHGFRTAIWISMNAVAQILCTFLIYGIGKNTHLSIAVWRIAFIVCGCVTLVAGALFLAFVPFTLKNAWFFNDRERQIAVKRLLSESDQGEKDLWDWSQARECIGDWGTWFSFLSGILVTITCGPIIFSSLIINDMGYDVYKTLEYGSPSGGVQFLCIWVGVLLLYLFPKQRCVVISVLILIPIVGNIMLLVMRERGGWGVIVGSWLGSCITSFYAILLSLNASNIRGNTKKSIVNNMFYIGYSVGCVIYPQWWNYTKDHTYKNGLIVDIAAWAFMEVVIYIYRFKCIHENKRRDNLSEDEIPHYPENMDLTDVQDLRHRYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI60</accession>
    <name>A0A1E3NI60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_73316</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016932.1">
        <property type="nucleotide sequence ID" value="XM_019164446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI60"/>
    <dbReference type="STRING" id="763406.A0A1E3NI60"/>
    <dbReference type="GeneID" id="30181133"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007223">
        <property type="entry name" value="Peroxin-13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF150">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX PROTEIN RSC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04088">
        <property type="entry name" value="Peroxin-13_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="889"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="11">
        <location>
            <begin position="42"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="12">
        <location>
            <begin position="304"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="308"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="953"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="545"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="921"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="932"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1045" mass="113947" checksum="875D852DD731B60F" modified="2017-01-18" version="1">MAAVSDTNVPEATPPAAAVGGASTADLYTQANIYLAKQTKPVIIPSFAKWFDINEIHEIEKKSLPDFFSAQSNIRYKNPAIYKTVRNFIVNTYRLNPIEYLSITAIRRNIAMDVASLMRIYGFLAKWGLINYQIDPKTKSFSMGPQFTGHFQITLDKPTGFALQSAESAITVKEDDEVELNENMDLDSTGIKVVSLNNNTHEPSFKRQKISNSVTPNVKLNLQLRKNVYDSVDDVFILQDSSNGNSKQVKRIYCSITGNDITETKYHNLKNKSNISLECFENSQFEDYFKSSDFIKLEKTNLNNEFRPWTNNEVLLLLEGIEMFGDDWNLIAGHVGSRAKDQCIIKFIQLPIEDTYLFKHISKSSYVEYLKKLKDDKTIDLSSLLSVITNGASGDSTTTTTTTTTTSSTTTANSAKRIIDNVHGHASSELNSQDNVITSILKLSLRKFELKLQGFMNLESRISEQQKFVETEKENLVLDKMKVKRQCLKVRLQLMKASSIARETKGNPSITPEALEASITEALNLVDRAIGEANYDPNASSSLAIFGQDGKSRPEGSQEASVQNVKENEVDVTKPISVVEPKFILQHVTAKTKTLGNGVDGDDLCVTNWLNATINNNNNVNIYNKPIRKYRRRNSITNSSNITNGLSGYGSSSYGMNGMGSSMYGGYGSYGSGYGSGYGGYGSYGGMGGMGGLGGMSSYGGMSGLGGYGGYGGLGGMGGRYRQMGYGGVAGEENSSGSLNGVADATARTFQVLENVVYAVGAVAALLESTYYATHNSFFTILGVADQLGHLGSVGGMIKDTFGGVTDSVKQIGNGASGSNNGPLGLYAVLAWLKRILKKLLGFSTASPTDSSVSGSHALLQEFSNWKNGLISNNPSLKSKNSKLSLKPLLVFLLALVGLPVLMSKFVRYIEREQQRRVQGPDASISAGLKADNTTATNADQSGHGRSNKIDPRSLEFARAVYDFIPEREEEQPNGWKELKLGRGDLVAILSNLDGWSHCRARDGRVGYVPTHYLEVIKRAEKTVAIADSPAKEESSKQTTVGKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIC0</accession>
    <name>A0A1E3NIC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_73372</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016995.1">
        <property type="nucleotide sequence ID" value="XM_019164461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIC0"/>
    <dbReference type="STRING" id="763406.A0A1E3NIC0"/>
    <dbReference type="GeneID" id="30181148"/>
    <dbReference type="OrthoDB" id="441444at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_uS15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15, BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321:SF26">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="36052" checksum="9B358263A79C0C3C" modified="2017-01-18" version="1">MFVNSIRKTVAMSSSSSMVVPLAQLPTRGFHATGITSISKAEKKLKSIEKKKKNIAFKNAVESKKEKVDPVLGKPDNAFIQRLKLEISEPNILIKGYNLADVDKLLFGAKEIRLQKIKEQDPSNVTLELTEKEEREKRDVLLRIMTLRNADNNEKQKKLTTLAMLEFQRFEGDTGSSEVQAAVMTIEIYNLMDHIQKNPQDLLHIRKVRMLTQKRQKLLRYLKRDDPQRYFWTIEKLGLTDENVHMEFNMDRKYMDEFEIWPGRQLVKINKRENEEMKKKRRMEKQALRRALHAKTLEKAAAEASAEPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIE7</accession>
    <name>A0A1E3NIE7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017025.1">
        <property type="nucleotide sequence ID" value="XM_019161493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIE7"/>
    <dbReference type="GeneID" id="30178180"/>
    <dbReference type="OrthoDB" id="1918at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014848">
        <property type="entry name" value="Rgp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12507">
        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08737">
        <property type="entry name" value="Rgp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="710" mass="80710" checksum="4EA57052DE1D1F65" modified="2017-01-18" version="1">MVTYRVHQKQISDRLQVQFIYENSPVFAGTDELSLLVRFKYTGDISNINLGVPLHDPFTGANSLREDNSGRVQVHTSTSAEEQNAEPPKDNDSSSGWFGSRLSSQLSNATRSLFLQNLNSLSEEDEESNGLTIAKKAQEEKRLTLFLGYAQILGHYLINDTIIDYSIFKDLEKSTIIEGKYAGIQGLDAVSQESDNSGGLLSGLSATYNTEMNSSVDSKMVEKMKFIPFYSTNQNIIFSELVFDPETWTATSQHTESVKSFYINCQLPKDLPPTYCTEAVQINYNFILGYQMMEGNNIVSKTVFVPLKIQPFIDNCGRQPVYHLNKARLNTYMEDLTADDVSSHLSSVKSDSFFQSGNILPNGQVTQHQPSRRISFWNLKRKLKKSKSRSSSISTVGSGLEKRRLSSVTSISSVEKLDEKVSEFLDILDSLDQADVNNIIQIQEQFEKKMNETKSFRFNVRENLMQIMADYKYVQRQKLSKKDFDDHLEYDYLLPKEQQTKYIIKQNHGIISTLFLNKGIFKLGDLVSLNISFAECKYKTKGIEVQLLKHQIFHRDEYLKKGNYDEVSSRLDKNILETILYEKVMSAFDATSINMDILIPVEAEPQFKTNFFISKYYLQVRFIIIDSMGKARNAEAANHAAGNGTVDEEAADAEGKKINCDLRNIFIDTTGSMLFRAKEHLSNANEFYIRIPLVVLPTYEQDFGIVTTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIK1</accession>
    <name>A0A1E3NIK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_17197</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017057.1">
        <property type="nucleotide sequence ID" value="XM_019161504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIK1"/>
    <dbReference type="STRING" id="763406.A0A1E3NIK1"/>
    <dbReference type="GeneID" id="30178191"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="134"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="547" mass="63273" checksum="E0C475EEBEB4737D" modified="2017-01-18" version="1">MTVVDSINVVTPQSAMAGEDKINVDTVINKNVGESGLVSRDSMDVSQEDYEGTIENHYDHDYSSDGIDNQNHQHVMDHEYDYNAADVVTLNESMEELKLNKYHYNYASAIDNLSSDDVGLMCFPIQKRLQVYKNGSNFTLLCVGESGVGKTSFINTLFDSLLIEKKSSYNEDFSQHPHKTTVITHHKMDLIEDGFKMRLNIIDTPGFGDYIDNKYCWYPIARYIDEQYRRLVYQENQPNRSNLIHSEVHACLYFIAPSAIGLTALDIDAMRNLSTRVNLIPIITKADGFTNEELSAFKQRIRNTLKEENISICELIDEKSRIKSLINEMPFSTINSVDVYANDNGDNVRGRKYKWGLSEVENPKHCDFLKLRDFLIESYMGDLISSTENYYENYRRDFMKYRLGKAINLTINEEEMEKLGIKEYDREVIVPSSRGSWKSISKDTSPSHPSLDQMIDTKSSIEILELLNRISLGETEKELVELNPAYLEMEKQVKKKFTGIVQLQNQKFKDWKRTLFEKQDKFNKDIEQVHHRMIALQENVKSLDFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIM9</accession>
    <name>A0A1E3NIM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_73483</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017118.1">
        <property type="nucleotide sequence ID" value="XM_019164493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIM9"/>
    <dbReference type="STRING" id="763406.A0A1E3NIM9"/>
    <dbReference type="GeneID" id="30181180"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="938" mass="107071" checksum="C563784568389AD7" modified="2017-01-18" version="1">MPQPYYVSAIAQGPDYEVLDTDDHAHPSLQSKVVIVGDSGCGKTSLFSSYIRGSFPLAYEPTIFENHRTFVKQVQSKTVLSADLWDTAGQEEYERLRRLSYQDADVVVVAYSVDAPESLPNIHEVWAPEVMTYAPHASIVLVGLKDDMVHAVSPAHAYQVAESIGAVAHLACSSIRMHNVNELFNCVFNTAYNKARMKKHHSTKQAQQLCPKEVQEEEQMYNPVITAGLPPHPPNASFLCIYHYDFHQKKDYSLCKEILKIVKIKQAFFLWLDPNDVSVALYEVSAIHPPSEPSPTYGHPMTKHSTVLLHAAKKLKFVLLATATLICFLCLRSGENRKPTVAETIHNQASVDFFSPELAYRPIYKVIEDSYSVEDFAKKPLAEKCYFYFERLHAYNPNWWTSPKLGTEYTHENLQKSEDLVHMNIYNHCFMGNATGSDPTAYEKLHVLFNNIDTRVYPYLSGKLPLFQHWTGSIHVGPPLLEAYPQNVAESVIDESTFAYDPSQLKTWESSSIYPKDKPFWNHYKDKLNNVGIVLSVNDALVEETMALLSLLRTLNNELPIEFLHRGDLSSLNKYRLFQVARAIKAPTTLSTFPKQDIWFVDVSFCLSDNYQNEFPKFFNKMLAYGFNSFKTMIMLDTDVVLFQTPASLLDTSQFKQSGTLFFKDRNVDMHMSAAYIDFLKNTSPNYIDSFIFGLIPVRKETWEGEYFKERYFHYMESGVVVVNRDKYWNAVILSLHLSFIQSTMIGSWGDKEHFWVSMLLSGLDDYKFDDYWTASVGSPIRNEDGVKYHKICSAHPSHVLSDNNELSWINSGIINCPKVSHQLASKDFDLLKEISNPHGRSFANVQELEKFYTTPIAFDAFIIPPLAKNNIGNSNLEDGKYSNLGLQQLSTCKGYTWCSYDTIGEGQTPEWKGIYKHFTGKQIQWYNYIAESYVKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ90</accession>
    <name>A0A1E3NJ90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_11227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017325.1">
        <property type="nucleotide sequence ID" value="XM_019159771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ90"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ90"/>
    <dbReference type="GeneID" id="30176458"/>
    <dbReference type="OrthoDB" id="21254at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF10">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="2">
        <location>
            <begin position="9"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="220" mass="25622" checksum="5292D6D3812BCB73" modified="2017-01-18" version="1">MGSENLTPKKVITTLSLFLRVIIEQIWYHFEIYPKESFRPYTFYGFRVFSSRHPGVISYLDQLELQLQKLMERGILVRVFLEIYENKKRKYSFGFSFKSSLLFEQLENDTQFVKFDASTDIFNTFTLVNELKSLLFSIINVYSGLSIAPSEQSDSFKVLISSSDDVHLSTDGNWILEKFSPNQPTISVTDEKFRDIDFQPLQDVNLGYLNIKSYLAVHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK71</accession>
    <name>A0A1E3NK71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017650.1">
        <property type="nucleotide sequence ID" value="XM_019164244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK71"/>
    <dbReference type="STRING" id="763406.A0A1E3NK71"/>
    <dbReference type="GeneID" id="30180931"/>
    <dbReference type="OrthoDB" id="3997480at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="656"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="909"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="664"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="909"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="919"/>
            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1007" mass="115449" checksum="58BA32362E0C8991" modified="2017-01-18" version="1">MSRLAHSELHLTFRDWSSSESEEESYMPDSTSHGNPDQVDDRSGDDGDQFGTGTSGIREVSRGRHRNNLSDRELDNEERNHDGNNAEIDDDGNSDHDSIYQYNEGNGHNRSQINQTSPLVSLPLPLVYNSTGMVDNTSFTETFSPAMQEDENSNSSTPPHLLNGTINIEDIHSTDWIPRILHKDYNIRQSAFAGSSFFKYHPEHEFCYGIPLSMQKYGLINMMNDLLLTRSECVLSEACKTESQADDKTKKRTFNQLCLEDMNLAFLMDQSYSSKIQQLAPTSFLKSGSCFSLPVSLSGSFLSVRFTDVDYKALNTTGYLQLGDVRLGFTGELVDILKSDLRYTKNRKFLIEGNVFSNLLRNKLVLSKKFNKCFDRKRKKCYTKENWKPFSMNRMGYIVSNKPSSQSLSNERTIINPVKPSKFPNGSLFHKLQVTSESKFDNFKLLSKWFEMPPLNQFINTPSPPTPPNRQKKCTNNPENLLVCKDCVNLMMSKFLFIKMEVDIHELFENSFDSSTNGLIYPDLIYKHRRRFHDYANRLMSKSNSNFRRHLERQMRGMTHTSNSLFNDTRVRPRLSSFQSSGDDDSDDEGNRGVRLTTQGSFGNRSNLGSEESQNDTGFELNPVAYEEYDDDQIDLDFNWEDSIDFPEVNNNEAMVSDSEENDVGANDGNDDGVNTSGGENDNVSDDERTTSQYNGPENSGMLAVWPLSAERFSESHPILDPDVSSNEGEVTDEEIDHPYNIIFTNDRSRRVSRLLFNINMASEQHRHRREPVSSLSLYKNGNTRMKTIFLACINRNTGELHILPGNLDMNMWSNEQNDGELFEDVETFNKVYKLFMVNNNLETEKDSKYSRRLKNWIRTHRKTESEEIKKLLMLQMLANPSIINGYEKRKTAQRQKVDEKRRRRQLNREKLNELEKKSKQHGVKKKKGKGNEKVARVAKEQQNGILADCGTAVDQFNDDVKEQLYSFVGETSSFSIDNDKVLTFQSGLENGKDKQRHGSLFSFEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKJ3</accession>
    <name>A0A1E3NKJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Uridylate kinase</fullName>
            <shortName evidence="9">UK</shortName>
            <ecNumber evidence="9">2.7.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:UMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxycytidylate kinase</fullName>
            <shortName evidence="9">CK</shortName>
            <shortName evidence="9">dCMP kinase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Uridine monophosphate kinase</fullName>
            <shortName evidence="9">UMP kinase</shortName>
            <shortName evidence="9">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">URA6</name>
        <name evidence="11" type="ORF">PICMEDRAFT_16457</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and dUMP as phosphate acceptors, but can also use CMP, dCMP and AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Predominantly cytoplasmic.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.14" evidence="9"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017719.1">
        <property type="nucleotide sequence ID" value="XM_019161214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKJ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKJ3"/>
    <dbReference type="GeneID" id="30177901"/>
    <dbReference type="OrthoDB" id="442176at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000315">
        <property type="entry name" value="Adenylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03172">
        <property type="entry name" value="Adenylate_kinase_UMP_CMP_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006266">
        <property type="entry name" value="UMP_CMP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01359">
        <property type="entry name" value="UMP_CMP_kin_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="9">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="9">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="109"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="187"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="289" mass="32095" checksum="0F7274E9D989F4F2" modified="2017-01-18" version="1">MFATKPGVRFVRPVVKSIGRNQILSCRSIPSCHYSTGAGGQGGKANHFLPLTLLGVAAVGLTYYNMNNNTKPPTPSIDPTSSTIQGSPLFPSEKYPEAINVVFVLGGPGSGKGTQCSKLVKDYKFVHLSAGDLLRKEKSTPGSEFGDLIDHYIKEGLIVPQEITIQLLKNAIVEQYDKGNRKFLVDGFPRKMDQALSFEKTVSEGKLVLYFQCPEEVMLQRLLNRGKTSGRSDDNLESIKKRFTTFIQTSMPVVEYFDDQHKVVKVDCNEPVDDVYNHVVSQFKEKNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKK1</accession>
    <name>A0A1E3NKK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_72673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017729.1">
        <property type="nucleotide sequence ID" value="XM_019164270.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NKK1"/>
    <dbReference type="GeneID" id="30180957"/>
    <dbReference type="OrthoDB" id="418242at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23958">
        <property type="entry name" value="SCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="2">
        <location>
            <begin position="1286"/>
            <end position="1471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1583" mass="182014" checksum="219273FC1FB464BA" modified="2017-01-18" version="1">MSKRRREFAEILDQTTPNTSLPYHPLSFLIPKQYAVPLAVPYDANIPSDTITFSQNDYNDETNKTIPVSLLSSDIWKGEDPDLKFVKFKKPFIQNRATADSDKSESPVAQMLNNTLTENITPVTKKDYFKSNLKVNKSGPITQPIDDEHLLDEINKLIGADVLEEPDATNSFSRVISREMEQEQKPEFIYDSAVTQSVLISHLIDEFQQTISSLCEGNVGTETENDTLNMWDTYTTIFADELQIVRPEILASLKSKLSELVTTNSHVQIEQTVFSKLLDICMNSVKEALSLDWEFVYESEDFSNTNEYQRFAEVVMNCTSIIILLYSNNLVEKLTRHESGFSLVVDFLCIYAALLKSLYLRETFTELPDFFISTLKHFISTISTLSDNITKLPLDESSVTRLEYISFDIIFTDILSTRERLNLNLVLEEMRSNFAHLIIAIYSSYNDQRVFIFNEIIENFGTLNPLKSKAKNYRLDCGISVQLISYLIVSLIQSHNDYGKDFDYSQYEFLTLNHPTKAKKLELQEINDNFWNSVWRQTDSMMKAIDLFITSFLKKLLASYSPSLRKIVENLMADLFFMLELPEFPSCGIILNSMLSNLLNICSNTDITLTSAHTLFFEIIGSIGSKILALKGSSDVSILDVRIDSSSLEILIEDLLTILSYFKFSSATRFHNHSVFNFESLLFLGKLKKIETHINTLLAQKKESLLQKQLESNKMVLRSVESALMKLMELVSQNKSQVDIEILSDTQIREIYKNLLLSQELMDRYNDVLTFILSSLNHPKAKSRSLATKNLTLLIDREPGLIQDAKLRYMIKHRLMESYASVTDAVLDLLFKVLESKPQYIEEYSDIVSQKMSDPSIAVKKKTAALIKYMFSNTRSVNVKVRLSQALLNQLDDEEDRITDLSCYILCELLFVDIGHLVVDNNVTDPVTIQNKAYEVIYVLCGIFLQGSATWILFERFFNEKVIYPSDFNESFRLELKSSLGLLIECMLELITNSYEQENVKEKITSESIMGVMSTFVKCDERLISQDQLITIQPYIINDYKGGSICFYALEILNLALNHSKTLNKSFVKSCKESLMKRLTKFNSKELDRAIQCLWKLFLLDDDTTSVSKACISSLRMLLKYIGDLQRFSNNTSTDSIVPRLLYLIGDFGRYCNFERDRQLFLSSKLGLLEKEPISVFLLKYLLKFCDTSISKPLRKIAIKNTLNVCISHPKLFFSVPVSKLIDSTFKKKDLEITNIVIGSLLTFLENEEIQMIKKNGLDTKRSGSIKLDVAVFHGYTLSYVNDGLCSTLVQKYMGNILHICLEPENAMNSIKFLGMVIKFGFCNPKLCFPTVVSLESAKSNHIRHIALELHRFLFEKFETLIESTYSESIKLTARYVSNVYRLDELTNCSNFLKMFFRVVKERKSKQRIDKFIQAILRALKLVNIYKFQKMTREELIITQTQIMFLCININEMEFTTQHEILSVIDCIEKIILTEESVFSDQFHLLLDLFEDDDNDERLRHSAMAKSLVSLHCLSKCLMENYSISSELILKYQESEDKKEFWTHVPQPDNKKFFVGEIKTLLTENAANQLTLLYKKLQEFAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL28</accession>
    <name>A0A1E3NL28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_34051</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017968.1">
        <property type="nucleotide sequence ID" value="XM_019162862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL28"/>
    <dbReference type="STRING" id="763406.A0A1E3NL28"/>
    <dbReference type="GeneID" id="30179549"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="6">
        <location>
            <begin position="14"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="97" mass="10703" checksum="1FFC6FAACB12434B" modified="2017-01-18" version="1" fragment="single">CKTDSDLTMTLENGILIDSHKRIGSIVANRQFQFDGPTPQSGAIYANGWSIADGHLVLGDDYIFWQCLSGTFYNLYDESIADQCVPVVLNVIDLVDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL29</accession>
    <name>A0A1E3NL29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SEC14 homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72854</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Microsome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017933.1">
        <property type="nucleotide sequence ID" value="XM_019164310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL29"/>
    <dbReference type="STRING" id="763406.A0A1E3NL29"/>
    <dbReference type="GeneID" id="30180997"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000013">
        <property type="entry name" value="Phosphatidylinositol transfer protein PDR16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF29">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0492">Microsome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="6">
        <location>
            <begin position="143"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00083195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="397" mass="44901" checksum="0B1D6912B63F4C72" modified="2017-01-18" version="1">MFGFGKKKETPKKVVAKNLVKVDIPFEAPLPGIQPPKEKPLNDEQNHKYLKVLKHFQDPELQVSVKEHDKSEKAPLIEDEKAWITRECILRYLRATKWNTHEAITRIEGTLAWRREFGIQYQDDTKNAVNSELVAPESLTGKQVVLGFDNDARPCLYLKPGRQNTKTSHRQVQHLVFCLEKVIDFMPSGQDSLALLIDFKNHPEISGNVETSKVPPLSVGREVLHILQTHYPERLGKALLTNIPFLGRAFLKLIYPFIDPLTREKLVFDAEFINFVKGEQLDKDFDGFVDFEYKQDIYFPALVEMTEERRKHYMERFNKFGAVVGLSEVDLRGTGDSVTIPVGRHPYNADEPEITATASAVSPISTTATTTTAPASNVGSDKKDVDEVVGATEKLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLK4</accession>
    <name>A0A1E3NLK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar RNA-associated protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_49487</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018138.1">
        <property type="nucleotide sequence ID" value="XM_019163139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLK4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLK4"/>
    <dbReference type="GeneID" id="30179826"/>
    <dbReference type="OrthoDB" id="277439at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006709">
        <property type="entry name" value="SSU_processome_Utp14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150:SF12">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04615">
        <property type="entry name" value="Utp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="497"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="672"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="748"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="152"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="769"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="946" mass="108441" checksum="98572BE52F5803BA" modified="2017-01-18" version="1">MARPQYRNKKESSRKKTVSPKATGGKPSFEFGDAVLDASRFLSNGSSNNSSRGRGGKEDDEENSGFQFEDEELDSDDALGSEDDLDDYMSKSSKRNTEDDEKLDEYGENEDGWDSVDEGELLTLSEIWDRDDKELNSAKKSSTKAKDHDLVFNDEEDEEDEGEEENSESESDDDDDDDDDDDNDDDDPFDEMNISEDDQNDIQLDSVMKNLKKNLPKDSNDKLRKQLINDNSAENEYSLPNQGDALTFDDMLEDIENLDGEENGGFNNDLIHDIAKHSKQLKELKEAALDEEEQYELNTNENSAFAIPLPINIQKRHDRRAAYEIQKEQVNRWREVIAHNREKKVLKFIPEKIKLDKTAAFKADSNIAVNDFEAKLDSIIDQSNTESKKTEDLFENIETAKLSKAEMLKRTNELRLMRELMYRGMKDSKRLKKIKSKAYRKQMRKEKLKENLLISQANRQEGIEDDDEEDVENDAVYKRARERMTLKHKNTSQWARQMIKSGMSKDKSTRDEMEEMLKQSEHLRLKQLGKKNADDSSEDERNLSDLEKDVDNMDEVDDEKLSKIGKGVLAMDFMKNAEERDRLVKLKEIDFLKKMNSDNSQNYGFQDDQSASGVNITMNSGRRVYTPSARVAAEEALKADQKLMEELDEDDSRSLQNRLSVKERNGVKIVENSEKRTYSGDDNVASGESANNDEENPWLVDHSENDDDSEDDNKSKSSTKLKIIDSSSSKLVKAGARIAKKLSKRKDLLKKERDDDDDTGHDFAAKSDDEFEDDVDEDDDDNVRIFKQADLIKQAFAGDDVLVEEFEQEKREVEEEEGDKEVNLVVPGWGSWAGTEAGADDGWGVPRNNKKRKIVKTIQGVVSKDKRLDKGKANVIINEKVNKKNTKYQTDKIPYPYKTWEEYERSLRVPLGKEWTTTQTHHKMITPKVITKFGSVIDPLKAPFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLQ8</accession>
    <name>A0A1E3NLQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_105860</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018173.1">
        <property type="nucleotide sequence ID" value="XM_019159694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NLQ8"/>
    <dbReference type="GeneID" id="30176381"/>
    <dbReference type="OrthoDB" id="166907at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19496">
        <property type="entry name" value="Elp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="304" mass="35280" checksum="29DD82E6CC0A1BD5" modified="2017-01-18" version="1">MSVSVASPTVLLNRIFSLREPCPFLLVSDTFVQSATFLTDELVHKARTSNPELKLVYLSFETVCVPSYIKEGRDVFVRLLEADFETQFGRIFSDKETLKHTLIVVDSFNYIPQTEIIKFLKFIMKPNHTVYGVYHQDIPPLRSNSDFQGPSSYSYLHFLSSCVFEIKPTSFEEHDSLYHRTLENGPCFPVGVHSIQQYIFFIHLTYRRKSGRSLEYNFKINSKTHKYEDVKQTLTEVTQDDESLLKDLTTFNLGTTKKQKEQRDKVDLPFLEAQKSMGSVGGSIVYEFEKDDDYDEEDPYEDPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLV7</accession>
    <name>A0A1E3NLV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_71237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018235.1">
        <property type="nucleotide sequence ID" value="XM_019163867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLV7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLV7"/>
    <dbReference type="GeneID" id="30180554"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="95"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="307" mass="34304" checksum="7BC93ABE0C93C55C" modified="2017-01-18" version="1">MLSVSTLLNLSHYVLSSGILLVSSISVLSLAALYVFQRKLVYPSTLNNARKVVDTPAKYQLAYEAVTIETDDGEQLQAFLLLHERKDPAYTNKTILVLSPNAGNIGLFLPVVHHIYENMRYNVLIYSYRGYGHSTGTPSEDGLKRDADAVMRYITSNNQLSQSSVIPYGRSLGGAVAIYIAARYGHQIAGLILENTFLSIPKVVPHVFPVLSPVSWLCTEVWNSEEDIKLVSPQIPCLFLSGSEDEIVPPEHMKVLYDTVSDSIDSQRSVVKIWREFKANHNNTIVAPGYWDTWEQFAREMVVPIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLY5</accession>
    <name>A0A1E3NLY5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_107896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018262.1">
        <property type="nucleotide sequence ID" value="XM_019159715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLY5"/>
    <dbReference type="GeneID" id="30176402"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="400" mass="43336" checksum="BEE0C9832DD5CB28" modified="2017-01-18" version="1">MMEKKNRQDRWIEGWVCRSICGKSDKLGPEGGGILRDRQDETSPKTGEGKRNGPGGQRCLWRRSRNRPPVRGKVIRGSRATSRTRPVPPHPARYARSAPAGRQRAGGPTVEERGRRCRLQPRSRPERAGSRGPRANKTASGAGGGGLTRDPSSRRGARSAERTRQQRLRQQRREEAAGAVAAGAVAVVLAAAFLVLALQELGQAACFLAGAPALLCGGRAGGGAQAHAEGVQRRVPRRGGGAGRGRRRPQRVAEDRAHPAEQTLEHNRPLLAAVPGAARPPSRSGPWGGRRRPLEPPPTVLHAHTHVHPGHIHVAAAKAHVRVKPPALGVVQHRRRRPAPAQLDLHTRLQRARPELHTHVVARLLKLARQLRVVLLPERCASSIGAHHVAHRQVLAVHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM03</accession>
    <name>A0A1E3NM03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Transaldolase</fullName>
            <ecNumber evidence="4 10">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_16266</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="4 10"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017486.1">
        <property type="nucleotide sequence ID" value="XM_019161142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM03"/>
    <dbReference type="STRING" id="763406.A0A1E3NM03"/>
    <dbReference type="GeneID" id="30177829"/>
    <dbReference type="OrthoDB" id="2015515at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000088">
        <property type="entry name" value="Transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00492">
        <property type="entry name" value="Transaldolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00874">
        <property type="entry name" value="talAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0704">Schiff base</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="324" mass="35659" checksum="F714770384A07DFB" modified="2017-01-18" version="1">MSSQLEQLKAAGTVVVADTGDFESIARFTPQDATTNPSLILAATKQAQYAKLIDVAIDYAKSKSSKPEEQASLALDRLLVEFGKEILKIVPGRVSTEVDARLSFNTEATIKKALEIIELYKSQGISKDRVLIKIASTWEGIQAAKELEEKHGIHCNLTLLFTFVQAVACAEAKVTLISPFVGRILDWYKAKTGESYTSETDPGVLSVRQIFNYYKKYGYDTIVMGASFRNTGEIAALAGCDYLTISPKLLEELANSKDSLPQVLDAAKAKSLDIEKVSYIDNEPEFRFLLNDDAMGTEKLAEGIRKFAADCVTLYDELLKRVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM25</accession>
    <name>A0A1E3NM25_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_108259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018293.1">
        <property type="nucleotide sequence ID" value="XM_019159720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM25"/>
    <dbReference type="GeneID" id="30176407"/>
    <dbReference type="OrthoDB" id="3998173at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="572" mass="64843" checksum="5F17775DA00C5664" modified="2017-01-18" version="1">MMISRVCTSLRDGVFMSPLCIACPSSTISGPVSLLPAGRAVCLYTYSIQLVQISRDSSHTVKEMPLTHVENGYVVCGVCHCDSNNYPKKTYYCPACIGFKLLKYNLNKINLQNVNTLAAHDINSVLEACFGDNATQFLTKYTDDYDKEERDKKLDGTNVSVGSVARLAFMLMNVDLLKKQEHIKSIGQLVEAKRRQSENLESRIKLLQEKRKGKQRLIALHRERVAAEMTVELQNIAEIKRLLNIGIVTRQNEYIALQQRKRTYDLMILWNVRILDKTKISVLFCPILPITQLASHSMDLVLNSLMKSCELVEAFARMLDMELPFRVETGYNDFTIGDFCYRLKDKRSLFELKHVQMLKFSMGIARVISNIVVLLRRVDTTYQFESTSLADLLSYDAMLVRLVTVLLGSEGVAELQRRMQVLERRDSHLPAIPSRPAAKGRQSSPWCIWLPSPAEETQPYATDVSLQTSVARYGLATMKEYNSVLSSSTVSGRIPDETPERSLAGTVRPSNPAIEAAELVHDERRLAEEVFCFLVTQMQAIRHENSAKDPLAQKHLRKPKAMKHESQMWVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMD2</accession>
    <name>A0A1E3NMD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DBF4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_10297</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018405.1">
        <property type="nucleotide sequence ID" value="XM_019159659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMD2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMD2"/>
    <dbReference type="GeneID" id="30176346"/>
    <dbReference type="OrthoDB" id="21380at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3410:FF:000001">
        <property type="entry name" value="Protein DBF4 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3410">
        <property type="entry name" value="DBF zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055116">
        <property type="entry name" value="DBF4_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013939">
        <property type="entry name" value="Regulatory_Dfp1/Him1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051590">
        <property type="entry name" value="Replication_Regulatory_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038545">
        <property type="entry name" value="Znf_DBF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375">
        <property type="entry name" value="ACTIVATOR OF S-PHASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375:SF26">
        <property type="entry name" value="PROTEIN CHIFFON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22437">
        <property type="entry name" value="DBF4_BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08630">
        <property type="entry name" value="Dfp1_Him1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07535">
        <property type="entry name" value="zf-DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00586">
        <property type="entry name" value="ZnF_DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="6">
        <location>
            <begin position="870"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="919" mass="103469" checksum="694AF5E2315559E9" modified="2017-01-18" version="1">MNKAPAACGNRPPLKDTSKHFVNTPTSKSLAAPVGPVKKHQKSQETHSLQPADLSVVHMQKLNTSDRTPFLMGTSTPKKDGFLPSLTSRNLNNSTTTSIPATRSTASIAAAKNTAATTESNIESTAANHASIVGNNKNHTNSSNNNNTVYKKMTGNELSKWQQTWREILPKSYVYFEQDESNERDKKRAMIALKKLGATVEPFFCENVTIIVSKRSYNKSAQYPKGDIFRYAVKKQLKVWNVEKVFRFLSHLGEQVPEVEEYDPTTAITTTITTTVNTISNNALAPQTAINSNVHNNNLSNLLMNERIFGPSDRDPSVKRNDFKYFTELYLYIWDVTHKTRPVAIREWKDKSLYPKIHHTTNGKSLFVPESRSQNAISILKRHQRRLQCLNDTFSYRQSIIESAYDIDIPNHKVRHPSYEERVAFKRKWEETIYKFNPDENPKLKYKKLHDSLNDKDKKQFSDIFPAEEKFELPLADLSSSNKRHKGFTLSKMSECESQDDENVDNIRTVHIIQKKYEKDNDGAKIISNSKHSNSKTGVGAQGEILIHDYNTEHEKNSNDQKANYDIDDYDNDVDINDDNYKDILKLVSDKVDVIDVINNPLINGTRATDCQNSDITKKNDLQEVTEQCTTHTNYMKRLPKLMREDSIINARSQNVSADGKLLCDYGEIAASGIQASGVNPSGTFSHGNAVGGIGNGLGPSKSQVVNKTLANEHKRIVVLTPALNSRQKNGGVLATINPETLKNMENMQQKESCGALLIADDTIPETQEQMMAGYKDSSNKTTINEDELAALRSVEAANPFISEGLLKQSKNDANKDIDENSSTRTCPKVGKQALKQDVENLALFKRQSKKNVTAAIGIFKEQKIERSKHEMKPGYCENCRVKYSDFSDHVFSEKHRSFAEDDSNFKQIDSLIDLLLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMD3</accession>
    <name>A0A1E3NMD3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pseudouridine synthase RsuA/RluA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018374.1">
        <property type="nucleotide sequence ID" value="XM_019163901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMD3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMD3"/>
    <dbReference type="GeneID" id="30180588"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="1">
        <location>
            <begin position="179"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="474" mass="55309" checksum="18514C185F7070FF" modified="2017-01-18" version="1">MFKSYVAQSIRRESSLVSDRLFKVVITPRKLQELEEIPDDLPRVTPQPNPPLFGIKDGYQSVAPYTKLTYTNVNRLKKKKPMTVMKLFETSFLGKNMDFYKKQIMDEQLWILRPTKDPLSSGKKKLRDADYSDYETLKGSDLFELQLKENDVVVNHACIHELKVPDSPNLNIIYEDKSLLVVNKPAGIPVHPSGSSYKFNTLQYLLTVKMNPLFDPHDLIDKDTLEFRTSLWPCHRLDKDTSGVLIFAKNQNRCSELTKQIENKKGMTKRYLALVHGKFGPKMRIVKDPVVDVDLAKRYENGGIGRKILYGKTTFKMIHYDEKSDTSLLDCHLFTGRRHQIRQHLRNLGFHIVHDPLYGIEGILKERMFHYPEKAEFLKLRKKYELQLQNRVNFWEKESICSNCYHVTYNDPTNHLDKYMRLHSIEYTYQPKDFTEPVWKFKTPLPHWAEAILPANKAAEFILPGSENENASHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMD9</accession>
    <name>A0A1E3NMD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_71380</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018407.1">
        <property type="nucleotide sequence ID" value="XM_019163909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMD9"/>
    <dbReference type="STRING" id="763406.A0A1E3NMD9"/>
    <dbReference type="GeneID" id="30180596"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="2"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="180"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="242" mass="27716" checksum="4B1A3EC517981994" modified="2017-01-18" version="1">MSAAKLLWKEFRDITHPKTGLKQVHVELPDENIFVWNVSLIVVDPESSYNGAYLSGQLRFPSNYPFAPPSFKFTPAIFHPNVYVDGRLCISILHTADNETSDEPVNFTWSPAQNVESVLLSILSLLEDPNVNSPANIEASICYRKQKDVYKQKIRADVQRSRERMPSDVVLPSIEEITAHKSAANGNEEENDEWWDDNFYDDEEDDEDDDDDDDDDDDDEVDDSHSDHENYVDMTDKVNTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMH7</accession>
    <name>A0A1E3NMH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_30840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018414.1">
        <property type="nucleotide sequence ID" value="XM_019162659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMH7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMH7"/>
    <dbReference type="GeneID" id="30179346"/>
    <dbReference type="OrthoDB" id="278329at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="138" mass="16521" checksum="5B0B0E4EE972E65D" modified="2017-01-18" version="1">MRETRRLLNASKIPFKVTFKKTKENENPLLPPLQLYRAIMRAHRRLPFDQRSLGDMYVKAEFRAHKNIDNPLQIIGFLSSWQKYLQMVLQNTDQDWKKYHIPDNMLEKMSDDQIIQLHELMKGTKSLYDEDAEETNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMK6</accession>
    <name>A0A1E3NMK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15321</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018475.1">
        <property type="nucleotide sequence ID" value="XM_019160768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMK6"/>
    <dbReference type="STRING" id="763406.A0A1E3NMK6"/>
    <dbReference type="GeneID" id="30177455"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="6">
        <location>
            <begin position="71"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="403" mass="45013" checksum="EA5B28F3A9590612" modified="2017-01-18" version="1">MLRLLPKRAATIKVFPTLARTLATEATTEAQNLDDVVDITLPEFSFEGYKLDPPSLDYQTEKGTLLQMYKDMIIIRRMEMAADALYKAKKIRGFCHLSVGQEAIAVGIENAITKQDDIITSYRCHGITYMRGSSVQEVLAELMGRRSGVSRGKGGSMHMYTHGFYGGNGIVGAQVPLGTGLAFAHQYRDNKNCTWTLYGDGAANQGQVFESFNIAKLWNLPCIFTCENNKYGMGTSAARSSALTEYYKRGQYIPGLKVNGMDILAVYQAAKFAKDWCESSNGPLVIEFETYRYGGHSMSDPGTTYRTREEVQNMRSRNDPIAGLKAHLLDYQIATEDEIKAFDKSARKYVDEQVKLADASPPPEARMDILFEDVYVPGSEIPILRGRIRDDSWSFEKGGFAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNA6</accession>
    <name>A0A1E3NNA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic peptide chain release factor GTP-binding subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ERF-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">ERF2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Polypeptide release factor 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Translation release factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_15525</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018702.1">
        <property type="nucleotide sequence ID" value="XM_019160862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNA6"/>
    <dbReference type="STRING" id="763406.A0A1E3NNA6"/>
    <dbReference type="GeneID" id="30177549"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002184">
        <property type="term" value="P:cytoplasmic translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000017">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000503">
        <property type="entry name" value="Peptide chain release factor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003285">
        <property type="entry name" value="Sup35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01343">
        <property type="entry name" value="YEASTERF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tr-type G" evidence="16">
        <location>
            <begin position="282"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="120"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="185"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="188"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ47589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="708" mass="79486" checksum="D171D7AD3437882B" modified="2017-01-18" version="1">MSDQQQQQQQQAQQNQFDPNNLAQGLQNVNLGGQFDPNNANAFVPNMNFQQFVPQFDPSQVQQQNYNQYQNYNNLNSNRGGFNNRRGGSNNYNNRNNYNNNQNYNNNYNNYNNYNNYNSGYNNNYNGGFNNGYSNGYQDTDQQQETSGSQQMSFEEYQKQQQEKLNAAPKKKTLKLNLKSSTVKLATKKKEEPKVEEAKEVAKEEPKAEIKAEAPKIEENVEKQVEEPKSEAPKAESKKETTPAPSVSASKLQNNTATAEALAKEQADQVDPAVVNDMFGGKDHVSIIFMGHVDAGKSTMGGNILYLTGAVDKRTVEKYEREAKDAGRQGWYLSWIMDTNKEERSDGKTIEVGKSYFETDKRRYTILDAPGHKLYVSEMIGGASQADVGILVISARKGEYEAGFERGGQSREHAILAKTQGVNKLIVVINKMDDPTVGWSEERYNECISKLSLFLRGVGYQKEDVVFMPVSGYTGEGLKERIPSSHCPWYKGPSLLEYLDGMPLVQRKINDPFMLPISGKMKDLGTVVEGKIESGHIKKGQNLLMMPNRVSVEILTIYNETEAEADNAYCGEQVRLRLKNVEEEDVSTGYVLTSTLHPVHTVTKFEAQVAIVELKSILSSGFSCVMHVHTAIEEVNFVQLLHKLEKGTNRKSKKAPAFAKQGMKIIAVLETTEPVCLETYEDYPQLGRFTLRDQGQTIAIGKVTKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNB6</accession>
    <name>A0A1E3NNB6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018704.1">
        <property type="nucleotide sequence ID" value="XM_019160863.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NNB6"/>
    <dbReference type="GeneID" id="30177550"/>
    <dbReference type="OrthoDB" id="6109at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="5">
        <location>
            <begin position="16"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="6">
        <location>
            <begin position="537"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="4">
        <location>
            <begin position="984"/>
            <end position="1318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1348" mass="152659" checksum="38E49F969B094934" modified="2017-01-18" version="1">MDVYNQFVYPSTSLFSIHCNFISKDQNNLVVSKGTLLQIFNVLCIEKEDANVNEENVSLPEEVFKPAYKLELVSEYALNGKILDIHKFRSNENEDVDYLLVSTETAKISVVKWNPSDFLVSVVSLHYYETVLDSLLMEKLSTSDVLHRTDPNYGCSAMFVDDLFVFLPFKKADIGDFDLNDDYKPTIDQKENSKISELDSALGSDLFEESFLVNAKTLHTDLKNIVDIQFLHSYPNPTLAILYAPETLSWAGYLPQAKDNMKVLVLSLDLETKSADVIIEIADLPYDIDRIHPLERPINGFLLIGSNEIIHINSLGATKGIYTNEFYAETSSLTLKNQSSLDLLLELSEVCQIGDSEVLLVSKSGKFYTLIFDEIGGISNLNRISVNSESTYSSFKVRSVLDITYISNKNLIFICAQGSDGLLLNWYHEPTAKETTSKSEIGADIFDADDDFWLYEDELNQNNEGLTSSLTSCTLVVSDSIINVGPVSDFTLGYTSVESKILGLPNPSYKETAIFASSGLDECGAVSIITPSVKPLIKSSLKFSNASKIWTVSNHKGDTKFLITTDQKTAKTQIFEVEKNYKDLVSKNFKNNSYTIQFGSIVTEKRVRPVQVLAHKVMIYSMGFGRVTSMSFEKEINHSVIHENYIVVIMTTGEIEILEYDDATKKLSKMDLPALLNFLIFTNAWISRSSLLNHATPVKKRAASGEPAEVKINLEEEVLFWLVTADNRLLVFRKEHLEKVFEFKNIHKMSEYLQLSPMDPNYEADVDPILKQCIFTKIGDEFNIKNYLVILTFGGEIIMYELFFDPVQQYYRFIKANDLFQLPITGAPANSYSYATKIERNLFKIDDLDGSNVVMVTGATPFIICKQCNSFPRMFKFTAKPLLYFAPFSNEKCKSGLITIDDKKSCRMVQLDLEFDYSNKLPIRKCEVGETVNKIVYHELSNTFLISTLKREIFRPKPKESEGEDASEPEIKNDYRIAAQNYRGSIKVLSPEKWSIVDTLELEENESCTTLNVLNLKVNGTDGNRVVIFVGTGIFQSEDVSTDGTWKLCDLINVVPEPGKPEAKHRLKVLCSETAKGPVLDACAVDGRFAVIQGQRMLVRMLKGEGNAAPVAFTDTSLYSGKVKSFENLMLVGDCYHSVTLYGFDAEPYRMLTLGKDENHMSLSESEFIVHNQNLYILASDDNSILHIFQYDPYDPNSLKGLKLLKRSSFRSNAHTTKMMNCSRRRSLFSMVSTLPIRKDVDLGYEVIASNIDGSFYKVSPINEYQYRRLYSLQNYIAEKEHHWLGLNPKMNAVGNLQDELTVIKRPFIEYRLLTKFSSMNEDKKRMFAMRLGKDALVDIYRDMISLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNC5</accession>
    <name>A0A1E3NNC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_22763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018717.1">
        <property type="nucleotide sequence ID" value="XM_019162275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNC5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNC5"/>
    <dbReference type="GeneID" id="30178962"/>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053020">
        <property type="entry name" value="Smr_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417:SF1">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="1">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="175" mass="19977" checksum="AA489EAFC639DBC5" modified="2017-01-18" version="1" fragment="single">TDNNEKDYNHATDKEYAKYREYAQQAYSKRAELAHRSQEAYKAGDKKQAGELSAEAKQQLAIAEQNNAKAAEYVFIENNRDSDDNDIDLHGLYVKEAEYILKQRIISGIRKNQPTLDCIVGKGLHSRNGVAKLKPAVEQLCQDANLKTWIDKKNSGVLHIDIRNARIPQDWYNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNF5</accession>
    <name>A0A1E3NNF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RecF/RecN/SMC N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_32072</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018750.1">
        <property type="nucleotide sequence ID" value="XM_019162740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNF5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNF5"/>
    <dbReference type="GeneID" id="30179427"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="13">
        <location>
            <begin position="7"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="276"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="754" mass="85728" checksum="4F7741CD69F3E777" modified="2017-01-18" version="1">MCHKAFSLMLGPQTNFIIGSNGSGKSAVLTGISVALGAKATDTDRGSSLKNLIMHGKNVARAIVTLKNEGPEAYKPAEFGPRIIIERTLKMEGAHTLKIKNSENKVISTTRNTVDAILEYFGITIANPMTILTQTEAKTFLAHSTDKDKFNSFMSGTRLKESFDNIKNIESNTNEIKDILTQNKTVHEEMKIKFKEARKVWNSFKDSDEYSKKKELLIGKKIWVEYKENEKLYLKASELLDKKKAEISENETKKSSITEDIVTMEKAKEELENGLLENYKEKYIRCKEEKDNSKNLITHAKEEIDEINSKLSNIKGNIDESKKLVTKIDKDIELEKKKVESSSEENVERLKSFQDKQKEKLADLNKQKEEMSSNMEDLQKNLSNIKQQSREISQSIEKTNDKLESLAVGTFRLESLIEEKENTEANLSLEVQRLEPIQAELHKAQKGMETQIEIYRSISEDYNNSRKVYEKKLKELSGFDSNIQFGKGNIRTLDSTNVKLESDVAKLNDYLTKTSDVMRTLETQANEFCSLEEAQLRPGVSTKSIDEEISGINRYLEEIENRQGITKELAELNLIKANSELKEIETKFSDTIGLYNKLCAALENRLNNLLQTTYLTFQEVESVFISALRIRRFRGKIEFNVKKGTLTLKVATKETAPLRSVESFSGGEKSYGQIAFLFAIWGPMHSRVRGLDEFDVFMDNINRRIALKLILSKVAENPKRQTIFITPLGVANVEGLDKKTVNIHEISPPERANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNJ0</accession>
    <name>A0A1E3NNJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_109147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018351.1">
        <property type="nucleotide sequence ID" value="XM_019159727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNJ0"/>
    <dbReference type="GeneID" id="30176414"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="84" mass="9655" checksum="AAD2620665E31478" modified="2017-01-18" version="1">MQEDRLGNELTHDQTSISILTQSNQSKNLKRNESRGQGVNLVDKHLADFDVNRYALRQQQRLNDRRSNGSIVEGTNTLQKLYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNJ9</accession>
    <name>A0A1E3NNJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018783.1">
        <property type="nucleotide sequence ID" value="XM_019163989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNJ9"/>
    <dbReference type="GeneID" id="30180676"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="124" mass="14236" checksum="F14683CDB40F5C65" modified="2017-01-18" version="1">MVRWSCHIGEEGNHHPKIMSKKSIVFGKNDGTSDNDVYDAFGNSIGKMWEENNYIWSQEEQNTSDVTEIECKRAGISCNLKVCGIENTSTRTRRKTNGKTKRDKIRVQGEEVSNSIMREKNHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNV0</accession>
    <name>A0A1E3NNV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein mL67</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial homologous recombination protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_15670</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL67 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018883.1">
        <property type="nucleotide sequence ID" value="XM_019160916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNV0"/>
    <dbReference type="STRING" id="763406.A0A1E3NNV0"/>
    <dbReference type="GeneID" id="30177603"/>
    <dbReference type="OrthoDB" id="5333655at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024629">
        <property type="entry name" value="Ribosomal_mL67"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28184:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28184">
        <property type="entry name" value="MITOCHONDRIAL HOMOLOGOUS RECOMBINATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12829">
        <property type="entry name" value="Mhr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="199"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="215" mass="25809" checksum="E272E3D9FC9F73E3" modified="2017-01-18" version="1">MATKFRNSFWLKTNEFAPQVFLFRNIETGQVVYSQTPHVTKYQIKQQFFRPNKENKKPLPRHDIWRPLAVAQFNDYQKAVQAYHGLVELRYMRQVSRKDESQSLRKLNDYNRIWCSGQYRPTYTMESTADLSAVIDELKLSENCKIYWDGLWWRGDAKHWNENIEHIDLERFGRREKFVILDEIREKGLLDFQKNSQLEEAQPESQTQPQQQTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNV9</accession>
    <name>A0A1E3NNV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018884.1">
        <property type="nucleotide sequence ID" value="XM_019160917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNV9"/>
    <dbReference type="STRING" id="763406.A0A1E3NNV9"/>
    <dbReference type="GeneID" id="30177604"/>
    <dbReference type="OrthoDB" id="408954at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102772">
        <property type="term" value="F:sphingolipid C4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="148"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="314" mass="37065" checksum="3293D4E925B26585" modified="2017-01-18" version="1">MQLVDYNDIVLIDGISQPVAKVTLQQSYIEGVPDTMIALVAPIVAYWVYSFYFHIIDVYQLLEQYRIHPSEEQLSKNRVTLPVVIRDVIFQHVIQTVTGLIVYVFESPPLTGFENAKLWAWRQNLPPFVPTVAIYLFYWYGISMIKIMTAFLIIDTWQFFLHRSMHQNKFLYKHFHSRHHQLYVPYAFGALFNNPVEGFLLDTVGTGIASLALDLTPRESIFLYTFSTLKTVDDHCGYSLPWDLFQKVFPNNSIYHDIHHQMFGIKYHFSQPFFVFWDTIFSSQYHAIDDYKEQQKQITLAKYKQFLKDRKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP16</accession>
    <name>A0A1E3NP16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DWNN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_24078</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018386.1">
        <property type="nucleotide sequence ID" value="XM_019162334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP16"/>
    <dbReference type="STRING" id="763406.A0A1E3NP16"/>
    <dbReference type="GeneID" id="30179021"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="7">
        <location>
            <begin position="3"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="365"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="428" mass="47851" checksum="A01FD63AC0DF25C8" modified="2017-01-18" version="1" fragment="single">AVIYYRFRSQKPDHIATIKFDGTGLTVFELKRDIILANNLLHSTDVDIVLYSTEDIQDTKSWGYQNGGSSSAGERELDDDNEVVPRSTTVLVRRTMTPKKNKGNVQRYVAGKPRLQVSGTNSVNKSISLGNNVGGTMNFGDAATNGDEDDMIKKMFSVQDEQWSQQQDVMATATRVDNFRTNVNEPVPEYYICYKCGEKGKHHIKNCPKNNDPNWEGVRVRKTTGIPKSHLKAIENPEDTIRDSNSSGNTTYMVNDEGKYVVAVADTKAWEKYQKTKKGESGGYLNGDVDVDDGELKDPETGKLWKSPVRIPCCNKIFSRKIIEDKLIDSDFTCPSCGKEQIYLDTLVADEELQAKVDEYVKNLSENKNNDGNSPKRRQVNPAGATANTSQLPQIPMMPMPPINMQMPPMNIGMPPFMPFMPMPGMNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP57</accession>
    <name>A0A1E3NP57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome quality control complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_120140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018970.1">
        <property type="nucleotide sequence ID" value="XM_019159867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP57"/>
    <dbReference type="STRING" id="763406.A0A1E3NP57"/>
    <dbReference type="GeneID" id="30176554"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="76918" checksum="0974B51E5B900672" modified="2017-01-18" version="1">MSSRALKRLGKASLEDELAKYQKKAESDEDESNDEGYNHGPGKMNAANAFAFLQGSEEEGEDSEESEESEDEKEEVAKSTKHVAFSSADKKTGKNDLKGKGKGKYNKGAKTVDDLDEDELDNILAQVKLEDKTLKKSNSEIVEDILSDASSVVDSIEQDLENEETLPPWPVYTSGGKLLTPKKFHRCSGLLQVDPRDLNSDREYENLFGKLSSAAIDDADSTSSSAISPEVLAQIKKLAKRIRGWSGRDHRSIPGTTRKLRLTKIKDDWLPIPKKPLSMDEVTSENLLNLYTTKFKNDWKEILQGDIAKEYKYGIKYYNVEPGPAFSIAATTEFFMSVVVQPDHESLIHLLQKCPYSIETILQVACILQRQGDNSNTNGLIERALFVFDWSLPNSFELGNGKTRLPFEYFLNRQIYLTIFRYISVLTQKSIFFTALTYCKLLLSFDPMDDPYGVRYLIDYYAFLSDEFQYLIDFVNSPLCRCYEEWLTAPLMYTVSLCYFKLGKAEEAKQALKDAYLRHPYTGHQILEKLGDIAHPWLLQDVNDAVKIGTAMYTVRLNTLVETPELKNFLTKELAAIIEKTGKPNLGAYYTNNLEKIPDNLIRNAVLSNESSVMAKIPESFWSDNDVYEFDVLPPQIGTTIYNYVDDNQISAGVMQNTMQAEEIRQLQDLLQQNVVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPC4</accession>
    <name>A0A1E3NPC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_15827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019074.1">
        <property type="nucleotide sequence ID" value="XM_019160984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPC4"/>
    <dbReference type="GeneID" id="30177671"/>
    <dbReference type="OrthoDB" id="10424726at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="30"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="409"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="281"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="467" mass="53247" checksum="9E23BC4A51EAB3A0" modified="2017-01-18" version="1">MASVPDLPTYKFIDISSFPRNKVGPLKKTLCIGRAPQKNSSRSADEKHNIYIRNPVISATHIELYKANSKFFYLVNMSKNGFFLIDEYDNNVSFIGDGDEPILLKNGNIIGMCFSKKFMMEYNKEIPENQRVEEALKQCKILVQIFTDYDQILVAKVYNVIDHVKGIQLQLDELVQEIGSECFRKSGLHPLTIENIISLSTEEYKYNDDAAELSRQKKMFYTAYDNSYDDFFNEYSESIYDSEAETNSDPEEPTTSFGFLLKDEGKVKEFAKDHDLGDKGLEEDEENEEDEENGEDNEEEEDEEEDEEEVAEEDEEEVAEVAEEEEEEEEEEEEEEGEEHDEDAESEEEAKEIEEIEGDVKSFQSVIISDDKSSLDNSIFSSSSLKRSFNESNCSDFDEEVGSVAEHKLHQLTELCNLQSKEIKQLSEEVKRLKRSKSQTKAAMIGAAGGAAATLTALYQIGSRMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPE5</accession>
    <name>A0A1E3NPE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_71934</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019038.1">
        <property type="nucleotide sequence ID" value="XM_019164051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPE5"/>
    <dbReference type="STRING" id="763406.A0A1E3NPE5"/>
    <dbReference type="GeneID" id="30180738"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015190">
        <property type="term" value="F:L-leucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903801">
        <property type="term" value="P:L-leucine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF26">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="44"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="529" mass="58415" checksum="81AD3191AC4F3B5C" modified="2017-01-18" version="1">MLSKATSQMTVEAQLSQAYHENKAGDEILDSGLKAGLKNRMINLIALCGIIGPGVFVGMGSALASGGPVGLLAGFAIVGILVLSMMNSIGEMNAAFDQNFAILASKYVSKGFGATVAAYYVIIWITNIIAEYTSLTSSLETYSSTVPTWGWYLIFWFVFTMFQCLNVSWWGECEYVLGFLKLAFLSGFYLFAIIYAAGGIPGHKPDNPFKNYPLKSGFKGIANSFVYAGVFYAGIEGISIVAAEARNPRKAIPTAVRSTVFRVFFVYFGLSIFYGITVPYNDPMLNGADKVMRSPMTIAMTEAGWPGSKYFVTTMIWITCFSSINSAIYFGSRALFTWSKAGYGPKIFTTVNSRGVPYVAIHFVHIFGFLSILSYSSGSSVAYSYIVSVSGVACFMVWTSISITHLRFRKGWVAQGYSLDALPYKAMFFPWVNYVAIVIGVVLTLVQSWSAFKPFDYKTFIDGYIMLPLFFIIWFCYDMFYFKSGFIKCSDMDFESGKRPDLDDIVGAELDNYCGESVQAAESTTEKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPY0</accession>
    <name>A0A1E3NPY0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA replication licensing factor MCM3</fullName>
            <ecNumber evidence="11">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_15970</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="11"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019263.1">
        <property type="nucleotide sequence ID" value="XM_019161039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPY0"/>
    <dbReference type="STRING" id="763406.A0A1E3NPY0"/>
    <dbReference type="GeneID" id="30177726"/>
    <dbReference type="OrthoDB" id="1882346at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904931">
        <property type="term" value="F:MCM complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008046">
        <property type="entry name" value="Mcm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056575">
        <property type="entry name" value="WH_MCM3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23191">
        <property type="entry name" value="WHD_MCM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="13">
        <location>
            <begin position="306"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="676"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="696"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ48150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="874" mass="97648" checksum="16B2832528DBDBCA" modified="2017-01-18" version="1">MEATAASASTDMAFEDRVRTFQNFLSIRVNEIPIYEREIQRMLKKGEKRLSVNIDELRNFDNNFAAGLLDTPADFLPAALKALNEMVHAIYSPVETPGITLAEDDEFYLSFVGSFGAYSLSPRTINSSYLSKMVSIEGIITKASLVRPKIVKSVHYNEAKKFFFSREYRDQTTSFDPINTVVTYPTEDAEGNKLETEYGYCKYRDHQVVTIQELPELAPPGQLPRSLDVILDDDLADCAKPGDRIQVIGVFRSLGGGLNDNGAFKVVILANSVYQLHALSTSTRVTENLTAKDIDNIVRLSRKKQVFKLLAESLAPSIYGHTYIKQAVLLMLLGGTSKTLDNGARLRGDINILMVGDPSTAKSQMLRFVLNTAPLAITTTGRGSSGVGLTAAVTTDKETGERKLEAGAMVLADQGIVCIDEFDKMNDVDRVAIHEVMEQQTITISKAGIHTSLNARCAVIAAANPVYGQYDTFKTPQQNIALPDSLLSRFDLLFIVTDDINDERDRKISEHVMNMHRYVPAGYSEGEPIKEKSSIRLAVGDFDVEESNGDKTSKVFLKYDPNLHAGITITDRNGVIRPLVLSMPFLKKYVQYAKTKQPMLTENAKNLIVDIYASLRNDENTKNSRTTPITARTLETLIRLATAHAKIKLSATISVKDVRVAEELLRFSLFKEVKKKTASDRSPRKKRKTDETGLSSDEEEDEEGEEDARYTITPSRRRVHTRSQDVRIHEDTEEQVESMRKGLEHLDTSPSAGRTRTVASTELTTETTEEPMNDAQQDDLSISEETTLKPVTIQENANFDGEISDNRYQTFKKTLSALLRNNKKDQYGIQELINSINEQLPQEDRFGPAESQAALHLLEIDNIVMTSENYVYPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPZ1</accession>
    <name>A0A1E3NPZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_72141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019273.1">
        <property type="nucleotide sequence ID" value="XM_019164110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NPZ1"/>
    <dbReference type="GeneID" id="30180797"/>
    <dbReference type="OrthoDB" id="433955at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF156">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="433" mass="48292" checksum="089AC51B8F0DBF9C" modified="2017-01-18" version="1">MDFDPLSFFEPEPKIAIQNTDNENVGKLHNNDITEVGGNSSETSEETGARKNEDCASGEKDGTVRLQVLDLPIVNTSLPAGVLSTLLKLFEPDQTKNFGPASQATHPTSAQMTMLELEKYLSCKRITTLQLEELSNFLKRNIQSTLTLTYLPKQDHTGITSFLTKVISFPFLNYTEEERDSIYNLASHVMTANSSPALKGDTTRVVEIEGLSKDILLYEPALTEDKIGNITWGASLELAKQIVKDKTSVWLPKPAAAMMTPILELGAGTGLVTIVLGLLGYRVVSTDLPEIVDNLTRNIELNQLEFVKSSPAEKIVTDSLVHVTSLDWRAPNEFLDRTMCLNGYETVLLSDPVYSTSHPYWVRDAVSAVLSQSENAHVVFMVGRRDRFEDVRECLWQLISDLGLKLILSRIVDGYDDYGVLQYDYKVYGRKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ39</accession>
    <name>A0A1E3NQ39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase B12 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_15973</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019269.1">
        <property type="nucleotide sequence ID" value="XM_019161040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ39"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ39"/>
    <dbReference type="GeneID" id="30177727"/>
    <dbReference type="OrthoDB" id="521512at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012576">
        <property type="entry name" value="NDUFB3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08122">
        <property type="entry name" value="NDUF_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6529" checksum="4199D5C315EFDFEB" modified="2017-01-18" version="1">MATLSDPWKKRDAWRYQGVFSKSQRFKGLFPGFYIGLGAFVAYSVYEDYLAPKPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ67</accession>
    <name>A0A1E3NQ67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_125338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019360.1">
        <property type="nucleotide sequence ID" value="XM_019159940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ67"/>
    <dbReference type="GeneID" id="30176627"/>
    <dbReference type="OrthoDB" id="3988936at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="1">
        <location>
            <begin position="204"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="335" mass="39054" checksum="8C452455D429A90B" modified="2017-01-18" version="1">MSFNCPLSNGELEEKKVQQESKELLTEFDAMPKAPTKVYFPESEFLAKQPSMIRLNRLVETKLRLKIHGFDNLAQRQVAISEGKYNRVARYRKKLARVDNTNSNQGRGVCREVVSEREQQCWWKIVLSPYFRENKRKSRMIYRLNECHSMRSMACLMGCIGAIIPNNLTLRHFLEFHGRYGWSYSTNPAQKMEMLPLRWLGEQANFLEPLVLRYRTDSSGRRLGFEGLCPYCPMQHEDRLNGYDSLFFNLRSCSYEAHLARKHGVYGSGEELSPPVFVQKDFEAKFVCMECQRVNSISGGPLDANSFQLEYFRHCVNSHYRRKSAGSANVQFQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ71</accession>
    <name>A0A1E3NQ71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sodium/bile acid cotransporter 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_21861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018806.1">
        <property type="nucleotide sequence ID" value="XM_019162227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ71"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ71"/>
    <dbReference type="GeneID" id="30178914"/>
    <dbReference type="OrthoDB" id="188035at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016833">
        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640:SF5">
        <property type="entry name" value="SODIUM_BILE ACID COTRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 10 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13593">
        <property type="entry name" value="SBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026166">
        <property type="entry name" value="UCP026166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41496" checksum="7A9B533FC6FB78F5" modified="2017-01-18" version="1" fragment="single">KVHRVLKRVFDYLVKKWFFIGLASFIALSHSFPHATEGVEIDYWAVALIFFISGLSISTKELLLNMSNIHAHVSVLVMSFLITSAIVFGICCGIKAAHNGEISEWMLAGLIVTHTCPTTVSSNVVMTRQADGNVALCLCEVIIGNMLGAFVTPALSQMYLSGTWQFANPANGTSVSSVYRHVMMQIGLSVFVPMFVGQVVQNIIPKQVKWCLRFFMLNKVGTFCLLLIMWSSFCTAFRQNAFTSVSHSSIIMICFFNLGIYLFFTLVCFFYARPWFVLKLFPHKPDENSSRVYRINYWLFRPFFYSKKDTVTILLCGPAKTAALGVSLVTSQYGNNNPYLGQLLVPLVLYQAEQVFTAGILTSFMKKWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQA2</accession>
    <name>A0A1E3NQA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GLC7-interacting protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019400.1">
        <property type="nucleotide sequence ID" value="XM_019161086.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NQA2"/>
    <dbReference type="GeneID" id="30177773"/>
    <dbReference type="OrthoDB" id="8436363at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055028">
        <property type="term" value="C:cortical microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043622">
        <property type="term" value="P:cortical microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31949">
        <property type="entry name" value="GASTRIC MUCIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31949:SF2">
        <property type="entry name" value="OS05G0480600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="500"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1075"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1285"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="296"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1298"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1317"/>
            <end position="1327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1404" mass="153402" checksum="E3FE3BC379372976" modified="2017-01-18" version="1">MAPARKDTDEEQNLDTLLSTESSGDFTDALNSNFLDPGVSNGDVDWLFRGREVKKLTKRSNTINNEVQSQLLHAQQKALVQSKHGRDSPTSSSSTSLQNLANSPATVDQQSASTSSPGAAGAVAGPAGSAGFSSSHYSAAQPNSAPVAIPIPSTRKSVNSSNVNLNSAVSANSLSDLPIKSSLKSASPANIGNGLKIDTSMIHSSSPHSSTSPSNNDTSVPLAFSASSSSHRSISSPMDPAPPASALVSSPASALNSNPNSTSTPTTPTNRSRSSSLLFGRSKSPSNASEHKPAKKSLLSSLSSKLKPGSHSTSTAPATSTSPSPAAAPKASAPSSPSVSRHPTPVAPHPVKISPPIHNNSSDNSLTKLAHVHNFENTHPTTSLSASDKLLDSVSFSRVSFALDGLVEDPQQQIPSRRPKRGNVLIPEDMLAPPPKLSIGITNSFNEQTKAERPKVDPKLYEQALARQNFFTAESKKHSEEAHIAALRMAKEVSSFKKRRGSFLKSDDDDDDEEEDITDSRFVGGDDKLDIDTPLHEHIDYFGHEENNSSSSSTNEETDRDISLETLYTRCCHLREILPIPATLKQLKGRSKPLHVLKMLNPKPTLIDILSFSDFLAIAPVISVILDNVTIDTEMLRIILISLKNSKMLDKLSLRNVPIDAEGWKYLCKFLRLNTSIQKLDISQQRVKKSSDHSKTVRSDLDWDLFTDTLILRGGMEELVIHGCCLSTDQLNNLVSKALVLKTKRLGFASAQLDVEKMSILAKWISTPNTTCNGIDFAFNDLSQGQLMPINNMLKQRSSDIKLQFFSLNSTNVRLKECSELIQNLSALPSLRFLDLSNNAHLFPDIIPVLRDYLPKYPDLRRLHFDFDNLTEFAIVQLCVIFQQCPRLVHVSFLGNNKITSKSASALYSAVKHSHIYNIDMDYDGLDEELVSRIAFYLMRNMENLLNFSVNPKCSDIKTSSTKSVVDSESIITSGVIAKDEDLIFDGSLLTKAAEHLLENGSHISGEERMVIYRALVEKTVKLRTEIHDSMNKLFEAREKGRLSIEGKENLLRFCLLDDSLENILDIFSDEVSAEKGSSGGQSNSSDNNGDHVSSRPGMIRRLSSQLTRHHSSEDVIQTGPIVSGEFYQHNDEIIPTVATEAPHIVVADADSVPVDSTTGKRVLMRKFSQTSVHSKKLEEEEGELHRWGFFVQQQNDIMPDDSQNVAKLKEEEKERERLRIEAEKAEKERLKSEAEKKKLKINTIPSGSELRETIMKAKGIESITDLIKKVNKDFTTVETIYKHADAKRAASRNQDTSSSGGSPISPSGKSGIGSGNVQSTKSSDISKISAADTDISAAESKTVFGEDRVSNRASSPDIDASAIDNTLSPTVSISSINEGDIKDQADEVYERILNNVIRVRSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQF1</accession>
    <name>A0A1E3NQF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_69984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019450.1">
        <property type="nucleotide sequence ID" value="XM_019163522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQF1"/>
    <dbReference type="GeneID" id="30180209"/>
    <dbReference type="OrthoDB" id="3996694at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133476" evidence="3">
        <location>
            <begin position="24"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="797"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="839" mass="94011" checksum="A5313C965AC7A30D" modified="2017-01-18" version="1">MQLQHTPLLPVVLLLLLLQHATALVSQDTLYDFDNRTLYLYLHNHDADDVSHYGSSLNKIYRIPFNTRLASGDVLNCTIAAPPQQACDVLIASAVLYSLCPSQHGLLGVSKYDHVSDSWKNVTLRSNIEYFSDATYLYTDSDRTGIYIFSGTPSSDSQSLSTRMIRLDTTTWESSNATSQVQPSPFYKSTALQINSNTLALFGGVSSDNQMVSMMEIPVWQYNSWAERPCNISSAQDVQPRTGSLVLPVFSETNQFAYNDTSTDFEVSSVLMIGGIGVNNTDVYPKIAALNVSRNVWEWEDLSNDLILADNSNAKTGVKLSLDSILAAGIIYDTLLTISVSPSSSSASNNSISMNKRSLSSEFELTIQLYNATNLKRLSEVDYTTIAASAASVRPFSHSNKNLTIALSVIIPILVIIILTCLVVWLYKRYRKRQEDERNEKEIREIVDFYENQHKQFSETTFSSYDSDYKSHGGSDYDFEKININNYDDSDDLSLNSWRRKRQDFQRQKSLFSIVKPKVDNLPDYSSNSVRRSLSVASNFISHSLKRSASTQSSIATFVTAKSSLNQFDIGNDNINNAKYEFDNNANINGNVARKNSFENDLYTIHSDESTLSPTLPSPPPAIPKHTTLVSFPNRTSSTLNHIPEEASVSTFHSQTLGFIPMKQAYNSSLVSLPQHHYMQQIFSPTIDSSLTNSSSVYSYNSKSARRPLSMISTSTMSSNVIVNPYLTQVHTENTRVSPPYNSNDYDEQGSIADDVLDNMEVQILVGSKRRSKLRVVNPDEDEESLKDENDIVDISKGLNKDRQIEEKSRCSSSSKEQGEDNEVRKRVISDEYHDESFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQH4</accession>
    <name>A0A1E3NQH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_32683</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019409.1">
        <property type="nucleotide sequence ID" value="XM_019162782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQH4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQH4"/>
    <dbReference type="GeneID" id="30179469"/>
    <dbReference type="OrthoDB" id="25887at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031134">
        <property type="term" value="P:sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14273">
        <property type="entry name" value="UBA_TAP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01770">
        <property type="entry name" value="UBX_UBXN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000002">
        <property type="entry name" value="UBX domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF20">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEP" evidence="3">
        <location>
            <begin position="190"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="315"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="43448" checksum="CD9F264257EEBEEE" modified="2017-01-18" version="1">MSSEERAMEEFIGITGIAKSVAEQYLSRNDYDVELAVNDYYNVEHVEPGSGPNPNLTGNKPATPKPSGPVFKSFSDLQHSNDNGPDDDNDNDDDMNFFTGGQKSGLAVEDPNKKKSGNGRKLVDDLLEKAEKEAGEPDWRDNDNTDEEKDVKKMKTFKGTGHSLGSIENAVDSRTILGENSSRGGSRPEKVTRIITFWKEGFSIDDGELYKYDDPKNQEYLKQLNMGRAPLSLLNVQMFQDVDVSVVKKLEESYHDHQKAKPRVYGFEGEGQRLGSPIPGEPTTVDEALEKYGVNADPAESVEAAEEPEVAKEAEAEGDTSIQIRLATGERLVRKFNSTEKVETIYEYVASNGDSSRPWGLVTSFPTQPLDDKKGETIAGAGLKNAVIIQRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQP3</accession>
    <name>A0A1E3NQP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_30232</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019540.1">
        <property type="nucleotide sequence ID" value="XM_019162632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQP3"/>
    <dbReference type="STRING" id="763406.A0A1E3NQP3"/>
    <dbReference type="GeneID" id="30179319"/>
    <dbReference type="OrthoDB" id="2877at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000017">
        <property type="entry name" value="probable 18S rRNA (Guanine-N(7))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039769">
        <property type="entry name" value="Bud23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022238">
        <property type="entry name" value="Bud23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734:SF0">
        <property type="entry name" value="18S RRNA (GUANINE-N(7))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12589">
        <property type="entry name" value="WBS_methylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="10">
        <location>
            <begin position="54"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23 C-terminal" evidence="11">
        <location>
            <begin position="218"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30853" checksum="C82F9DFAE467737E" modified="2017-01-18" version="1">MSRPEQIAPPEVFYNDTESRKYTQSSRVQHIQAKMTFRALDLLNLEEGRSHYLLDVGCGSGLSGEILTEEGHMWVGMDISADMLATALDRDIEGDLFLADMGCGVPFRPGTFDAAISISAIQWLCNADTNTNEPKFRLARFFNTLYSALRKGGKFVGQFYPANEDQTVQITGAAKVAGFQCGVVVDEPESKRNRKHYLVLQAGSSARPLNVDGMEVENMAKAQHSKESSKLSRKRTKALETKKEYIKRKKILMEKRGKTVRKDSKFTGRARKARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQT0</accession>
    <name>A0A1E3NQT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA protein C-terminal winged helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019564.1">
        <property type="nucleotide sequence ID" value="XM_019160189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQT0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQT0"/>
    <dbReference type="GeneID" id="30176876"/>
    <dbReference type="OrthoDB" id="511599at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056808">
        <property type="entry name" value="HTH_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168:SF1">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24913">
        <property type="entry name" value="WHD_AAA_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA protein C-terminal winged helix" evidence="2">
        <location>
            <begin position="441"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="647" mass="73781" checksum="C9391C7A7DA7B18E" modified="2017-01-18" version="1">MLLLSKVSVKYGGLHPNLTGKVLKQFHTTSRLALNPLLPIDPTEILTELNDEEKARNEEEIKRVKGRIKSILHKPKKTINGSKNTDRKGTSSTSGSNGSNGSNNGGNGKGNGNSDGPKLNKNGNGYWKQFLGIVRKCTEAGLITMASLGVLVLGGILYHKIYKRNVLWKMDDSFDNHESLMLLKHQENVGNSDKELWAERVNQKWIDEIVTGKIKGKYYLLLGEKGTGKTSTVMESINRVDGKDCVVVDCSSDNELMRLRIGNALNFEFSEDYIGSLFSMKGPRESTPILDIERAFMKLEKVLIERRRKTKKPLILTFNNSHLVDSSIVELLQQKAETFSSSGMLTMIFISDDYWLFEKLKTFATRLQVINFEDTQLEEASKILKSSRIKFFQESLDDQKCEAIYNMIGGRPQHLNHVASQWDVFDAAHKLIDSEKLWFLNNCALLGADMDDDVMESGKFATSAMLLMRELVEMDRDRNENKSTTEFSKLPQLPLWRARQVMTRPDYIEKYDKMNIFTIGTDSNVRADSVPMMRAFHEIASDPKFDSLLRETIDRISEIESLGRTRELIFKDLTSKGQYVLHKDPNSHDMLISVKTADENKIEESIVNIQNQEERSSDDDLHHEGEKKKWWKRRLETYSQSGKGIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQV2</accession>
    <name>A0A1E3NQV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_82835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019593.1">
        <property type="nucleotide sequence ID" value="XM_019164995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NQV2"/>
    <dbReference type="GeneID" id="30181682"/>
    <dbReference type="OrthoDB" id="10263353at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBP-type" evidence="2">
        <location>
            <begin position="101"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="473" mass="54323" checksum="E819A11AC239373F" modified="2017-01-18" version="1">MSRQKIHLLTEQNGIESGVLDEVPRKKQKKEIESEITDDGGNENENENEKGNGNGKAEESDEFDDLEDTVRTETVGLKYTDSDIYLDTVNRKVLNFDMPLICSVSLATTNIHICLVCNRYLQGASLSSPAFTHAVDTNHHVYINTQTSKFIILPEQLVLSENRSKDLRDIQLLLQPKFGKQLISELDSRPLEGKTMAGEAYDVGFVPLVNDYMSDIIDTRFDAKEGNSILKHNTLYYALLHLPLVRDHLLEYKNSESTPLSNQLSNLTKKIWSPYLFKNFTSSFAIEYYLLSCRLPTKILDDVRLFYTWILNTLMKENKKLYKDLFTGKLVSKGGAKPIKFINLTVKLPQQSVFKGSTSLSIEQYDLMTLIKEKGYQVLKFPQYLVIYIDRTNDVQLEGIEQQLNKSIVKFDPNTLQLNERTNYKLLANITDSKVQVLDKSRKRWMEFDGVGVKEVEKELLFIANSQLQIWEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRA0</accession>
    <name>A0A1E3NRA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCT-theta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14153</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019725.1">
        <property type="nucleotide sequence ID" value="XM_019160247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRA0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRA0"/>
    <dbReference type="GeneID" id="30176934"/>
    <dbReference type="OrthoDB" id="1748577at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="555" mass="60962" checksum="D0282BC9C3A4EA17" modified="2017-01-18" version="1">MSLKLPGNPNASLFKQGYQVHSNTDGAINRNIQACREISTMISTSIGPCGKNKIIVNNLSKIFITNDAATMIRELDIVHPAVKVLVMHSEQQEEEMGDNTNFVLVLGGELLHLAEKLVVLGLTPTEIIQGYKMANKFAITELEKLVIDQVNDFQDKDELLKVIKPVIMSKQYGQESIISELVSTAVQHVIPKSNPMAFNVDSIRVVKIMGGSLANSFVIKGMVFPREAESKLKKITQTAKVAVFNCPVDISASETKGTVLLHNADEMMNFSKGEEDELHKVIKEIHDSGVKVIVAGQGIGELALHFVNKYDMLVLKVPSKFDLRRICRICGATPLVRLGAPMPEEMGTIDVVESTEIGSDRVTIFKQSEETSLTSTIVIRGATQNNMEDIERAIDDSVASIKSVLKDPRLLPGAGSTESELVKRITNYGEKTPGLLQLGIKKFAEAFEFLPRLLCETSGLDPSEILPRLYASHDENDNETLKYGIDIESDDVNDCLLDVKKEKIYDSFICKKNAINLATEAVCTILSVDQIIVAKKAGGPAMPKQPKPGNWDQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRE0</accession>
    <name>A0A1E3NRE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Regulator of free ubiquitin chains 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_27596</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Inhibitor of the DOA4 deubiquitinase involved in the regulation of protein degradation by the proteasome and maintenance of a normal level of free ubiquitin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the RFU1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67C family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019765.1">
        <property type="nucleotide sequence ID" value="XM_019162504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRE0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRE0"/>
    <dbReference type="GeneID" id="30179191"/>
    <dbReference type="OrthoDB" id="3640at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08066">
        <property type="entry name" value="MPN_AMSH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000033">
        <property type="entry name" value="AMSH-like protease sst2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044098">
        <property type="entry name" value="STAMBP/STALP-like_MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947">
        <property type="entry name" value="AMSH-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947:SF13">
        <property type="entry name" value="FI19924P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="14">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="162" mass="18396" checksum="0CB28FF60D31D88F" modified="2017-01-18" version="1" fragment="single">LRTIFLPAELSDSFLQIAKNNTLNKLETCGILVGKLNRNAFFITHLLIPEQDSTSETCSTKNEERMFEYIDNEDPDLFILGWIHTHPTQSCFLSSIDLHTQNSYQIMLNEAIAIVCSPSDQFPKKLGVFRLTDPPGIPTITNCSKTGFHPHHEPNLYTDCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRI8</accession>
    <name>A0A1E3NRI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14266</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019850.1">
        <property type="nucleotide sequence ID" value="XM_019160302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NRI8"/>
    <dbReference type="GeneID" id="30176989"/>
    <dbReference type="OrthoDB" id="2261376at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001406">
        <property type="term" value="F:glycerophosphodiester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000140">
        <property type="entry name" value="Putative MFS phospholipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0843">Virulence</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="56"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="533" mass="58445" checksum="668B24087D1B448B" modified="2017-01-18" version="1">MPFKSLPGILRKEITWGRNILTEEDHQHLEVTDKDGNIVEIDEEDIAPSGKKNYWPIFTAGAGLFSDGYVNNSIGTASTCLSVIYGDQYTHSHALRNVSAIAFVGTVVGQLTFGVFSDYISRKTGMLVSSGGLIIFSILAAGCWGVGTEAKYGGNAGGLFAALTAYRFFLGYFIGAEYPTGSAACAEASALLPAGKRNRYFAWFTNFMIDFGFVVSAFVPMVLLWICGTKHLQPVWRITLGIGAIPPISLFILRLFFKEGKQFQKLNLKNATIPFFLIIKYYWFRLLIVSIIWWIYDFSAYAFGIYSTPILKQIIKDKDMYKTFGWNVVLNLFYLPGSFLGAISTDYIGPRLTLALGVFLQAIIGYIMAGLYPKLETEIGAFVTVYGIFLTLGEFGPGDNIGLLAAKTSATPVRGVYYGIAAAIGKIGAFVGTYAFPSFQNHYPGVEGYQVPFWLASSLAIVAGLLALFGLPAVDQEAMQREDFEFLSYLSQNGFDISTLGDGTLMSGEKHSSSAANSVEEEVVEIFDDMKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRJ9</accession>
    <name>A0A1E3NRJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14237</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019819.1">
        <property type="nucleotide sequence ID" value="XM_019160286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRJ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NRJ9"/>
    <dbReference type="GeneID" id="30176973"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="389"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="341"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="436"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="522"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="586"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="701"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="103"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="459"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="522"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="589"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="610"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="701"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="724"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="811" mass="88404" checksum="8223850A027EBE79" modified="2017-01-18" version="1">MDSTSIWDIYSSAKKLLPLYERMQNRSIRQESRSIQTSKKEVQNSALDSMLSSTNSSMNSSNPTADFNMNYMNNEDILSVDLLTPLSASPAAIVSASGKQLKNFNQNDNTNNNTNTNSNRNNSGSNATNNSSNNNVNNNMSNTANGNNNMITPTSITESPNSTNNMGIKLAANSNSNDKMFDPFKSYAFADIVDFKNDEDISSSEVDFDILRELKRINESNNLNFQPTQTIRPLDIKIKSESSISINNQNILSTNDNNNTISSFENEKKNVNNENNVIKNEAMSSESTPTGMTPNKSIYENDRMINATTNTGTTPSTMNNTFQNKIKDTPIINNKQKFIKMESDSPSPSSHPRMGSTSQPNELQTQARQSKSTISSSAPTKENIKSISKCSNCGTTKTPLWRKDPNGNTLCNACGLFLKLHGTMRPLSLKTDVIKKRNSKRQSLGAQGEGYKNLSHMTPNPKSSSQSPNPSMYFPQSFPNSNINNFGVTGPNQPPVQSPHQLQYIQPQPGLSIPQSKTFVPVHSQSQIPVNSGNQSQNRSKIVPILPKPSRDSSSPNQNFNAINFNFSIGASPSQPQDIPQFKRRKSRLNLSSSQNSQPPSPLTAINTGTSYSPSSSVTYSPVTNMTASSPAHASSPAGGVLLVSRSLSRQNSISSNSSHNLYQDLNNKRGLTYNNVNQIANGTPTNSIISNSYNNTMNNNVISGNSNSSPSNSSGNSNLSVNKCSQQRQQKPSNFSNLSAGMNAIRISSAGKSGLSKCLANVNDKPQAMQKSLKINNKNSTNLSPFDGGSKNNIDTGINMDDLDWLKFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRL1</accession>
    <name>A0A1E3NRL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosyl-DNA phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_56920</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019833.1">
        <property type="nucleotide sequence ID" value="XM_019163205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRL1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRL1"/>
    <dbReference type="GeneID" id="30179892"/>
    <dbReference type="OrthoDB" id="47785at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ48720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="71890" checksum="132063ED38D1BD1C" modified="2017-01-18" version="1">MSLSDGVINLESGSETEESDGVIVEQSTNGGKSQVIEISSDEEVTVPPEPEEEVVHLSSESPPATSKEKEQDEAPKKCSTEELTPQQQLRLKLAEAAERRLIQKTEPEEMPKKRQKVRSTEKDERLEVRHQTKLLPTSIDDRSSRIRLISNPDYCAQFGVVADKDTVTLSDLVGSKDLIRTYQFNMLIDFDYLSRFVTSKDCEFTLINKSDDDHLHVKDESWERYKIRTIDVSSKLRKYGTHHTKMMVNFYRDNTCQIVLHTMNLTEADHKIQTQMCWVSPQLEMHKDVNDWFDFNQPDLSLIDETGLVFKRDFIAYLLSYENNDINKLIDQVAKYDFNPIDVVFVASTPGHYQFTDWNTLTKPSSKPMFGYGRLWQVIHILKLQSLMGKLVGQVSTIAGPCDNWKRNVFVHVLTSCVEKGYPISKKADYKYVVNKNKVEPVIVWPTVTEILKSCAAPLSGIALHLTSRGKWAAYERQFLEIKNNFHKWSCTDDPKLSRAGRSNLAPHVKTYTVTEDNFKTLKWFLMTSANISHQAWGKPKGYNTIEYDICSFEAGIFIAPQLLKVMSNVKEKVQILEPTYGRDSVIDEQSLSGSVCKIGLRLPYDTPLKKYNATDKAWSQPDSDQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRS1</accession>
    <name>A0A1E3NRS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_8934</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019501.1">
        <property type="nucleotide sequence ID" value="XM_019165042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRS1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRS1"/>
    <dbReference type="GeneID" id="30181729"/>
    <dbReference type="OrthoDB" id="189997at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="10"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="159"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="782" mass="90398" checksum="E5BA219018FC70F7" modified="2017-01-18" version="1">MFLQPDSIKSCSNCKHKKVKCSGELPVCSRCKKSAASCDIVNNVFYNFQTVQSLLGVIQKLEDKVAEAETSQSAYSGEDQPNSVDQPLHAQPLDENQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLKQQQSREQISPQSQQNSSQLCSPPDVSTPNSTSSSKEKTNRKGTTIESISTEVGSLTISNNDNTQGKYIGAATGSNFARLFLKQMHLGRLNELNSSAMDDFDSFDSSVTKSYAPLPPYRISKYAVLKYINCVHIYYPLLLLQNLKHTLHVMYSSPKEVSLHDKFVLFIVISIGLDRAEKDVEMTNYINQFKPVEYFNTAYRYLEEILSIRSEKSLQSLLLVIIWLLNTNVLRDDNGDLWHLGRFSMSLAMELGVHRFNPDWDFGEMKNELRNRLFWCTYILERTIAMKFGRGLSLRKQAIDTPIPQLFKDDYISDNTNFGTDLLNVYDQVQFKPSLLLINICEIYGDTLETVYISRAAHSEPALSLEEISNYKTQLQNSLTQWMVQVDKQIPNTLECYYELKIRYCLASVILNRPSPSFPIPDTESILRCKTDCQECISSYCWMLDHGWKINPTCLHDLVNVGLTMIYCCWKTETDSNTLKDFSLKILNIMNEVVKYYPTFTKFKNLFIIVSSIIIDGFNNHVNNSGGAVGNSNGDDSDTMTEVQQAGGHNEFMFQNLPQQLQQVKSSVQYPTSFHFTNPQANTEFNDWFTQELFQDVFRQYYFPSNDTIMEDLDHLFNFQTDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRT1</accession>
    <name>A0A1E3NRT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_18718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019857.1">
        <property type="nucleotide sequence ID" value="XM_019162085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRT1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRT1"/>
    <dbReference type="GeneID" id="30178772"/>
    <dbReference type="OrthoDB" id="7396853at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096:SF0">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006157">
        <property type="entry name" value="Doxgns_DODA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="6">
        <location>
            <begin position="11"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="293" mass="32450" checksum="44EE1D61C54BAC4C" modified="2017-01-18" version="1" fragment="single">EFHKSKPQPIFFLSHGGPTFADKHDKVGSNVGAWNKTRQIGNYIKNELKPDYIIVVSAHWQSDAPNTIEVSIPGPDENALIYDFYNFPQKFYDSQFHTVTNRALADDIVKTIGESGFFKAKTRERGIDHGVFVPFKIAFADTEVADSEELDVDVPVIQVSLAANSDIETHFRLGQALSKYRDQNGLVVLSGASVHNLKDLRSPGPGESYEYAAPFNHLLTDILTHEDSSAVLPKLIKIPETPELNKIYVGAHPTNEHFLPAVIGAGVSRGDKCKLLYSDLIASLGWNLYGWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRT5</accession>
    <name>A0A1E3NRT5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_27647</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019909.1">
        <property type="nucleotide sequence ID" value="XM_019162506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRT5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRT5"/>
    <dbReference type="GeneID" id="30179193"/>
    <dbReference type="OrthoDB" id="18894at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="4"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="309" mass="35051" checksum="0209023D011DF936" modified="2017-01-18" version="1" fragment="single">IFSILLWYAFSMGISVYNRWMFSSENLDFRYPIIITSFHQLILSLLAICTLIMFPSFRLSTVKYRRYVTKILPCSIASAGDIGLGNAAFRFITLSLYTMVKTSALIFVLLWGVFFKLEKLTVRIFTIVCVMTFGRLFYEIRETGELKGSHIIFIGVSLVLLSACMSGLRWALTQITLKKNKRTKNPILTMLYISPGMCCVLFLVGTVVEGVSNFLNAEIWEAKGVGMTIVLILIPGLLAFFMTLSEYVLLQYASLLTLSIAGIFKELLTIFVSWVVFGDELSFINIIGLSITFADIVWYNVYRFQQNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRV5</accession>
    <name>A0A1E3NRV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_87614</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019948.1">
        <property type="nucleotide sequence ID" value="XM_019165030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRV5"/>
    <dbReference type="STRING" id="763406.A0A1E3NRV5"/>
    <dbReference type="GeneID" id="30181717"/>
    <dbReference type="OrthoDB" id="273070at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="371"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="371"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="262"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="501" mass="57037" checksum="83F5100CA97B971A" modified="2017-01-18" version="1">MTAKRKPAKQSGPFGGNKRKPPSEILRPGVGGLPAKPVFSHNDVRADKHKDTRNLSGVTNAISTDTANYRRPNNGSFEMFPGMIVDLARENEANNNSNTSNIPQPSAEALNLFSSILGTLQSSPQQQERTQVQQPPVMPQTLPQTQQHTQQQFVQPFPQQYQYPPQYNPNPYLASPQNQQEYVQNPVSPYQYPQNYNQPTYQNYPLLNQPTQPVQSKFDGGNQQSNAFLSMFNQLLESYSSSSNRSLMKNSNVNDSVTSQHQKNDRPQKPAKSDQGKQKKNKKLEQIEIKDDNGSDDDLLADEEVELEKKIAVQGTNIVLENEEDIQKWIEERKKNWPTNSRVEEKRKELENAKRIVETINNQTQPDEKSTQKVKMCNFWLRTKKCRLGKDCKFSHDMSNYSKPKNSNGKPRTKTLSTKPLPNHKLKLIHGIPVQIPSRFTPLTNEGKSLHNLLMEGERFQNENVELLGLFTRLVTCGIIRQDWNGLKKKLKLDGESLTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRZ3</accession>
    <name>A0A1E3NRZ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_69980</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:23701"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:23702"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:22813"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:22814"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:20222"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:20223"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019446.1">
        <property type="nucleotide sequence ID" value="XM_019163520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRZ3"/>
    <dbReference type="STRING" id="763406.A0A1E3NRZ3"/>
    <dbReference type="GeneID" id="30180207"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000245">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001859">
        <property type="entry name" value="Formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1510.10">
        <property type="entry name" value="Domain 2, N(10)-formyltetrahydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF26">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="16">
        <location>
            <begin position="41"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="17">
        <location>
            <begin position="178"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ48333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="983" mass="106668" checksum="C0C39160F4A54E32" modified="2017-01-18" version="1">MLRLPTRRLSLSLSRSVASKQVLAASRNFSISNAQLQATIISGKELAKKIRSASFDHISEQKSKFPEFQPKLSIIQVGERPDSSVYVRMKLKASKESGIDCDIIKLPEDIDEISLITLINKLNDDAKVNGILIQLPLPKHLDEVKITNEVLSIKDVDGFDRFNVGELSKRNGKPHFLPCTPNGCMKLIQETGVDLNGKNAVVIGRSDIVGTPVAAMLRNANCTVTICHSKTKNIPELVKNADIVIAALGKPAFVKGDWIKPDAIVIDVGINYVPDSTKKSGQRLVGDVDFESVKAKAQYLTPVPGGVGPMTVAMLVDNVYKAALSQFEEANKPLNIKPLKLDCLDPVPSDIDISRAQTPKLITEVANEMNIAHKDFENYGHYKGKVSLDVYEELKDKRENGNYVLVAGITPTPLGEGKSTTTMGLVQALGAHLKIPSIANVRQPSMGPTFGVKGGAAGGGYAQVIPMDEFNLHLTGDIHSISIANNLLAAAIDTRMFHESTQKDETFYKRLVPKKKGVRKFTPSMLKRLQKLGINKTDPDSLTPEESKKFAKLNINPDSITIKRVVDVNDRFLREITVGQSSTEKGFTRQTGFDITVASEIMAILALSRDLKDLRERVGKMVIGSNFDGEPITTEDVGCAGAITALLKDTIKPNLMQTLEGTPVFVHAGPFANISIGASSVIADKVALKIVGKPKNTAIAENFEKGFVVTEAGFDFTMGGERFYNIKCRSSGLKPNVVVLVATTRALKLHGGAPDVKPGQTLPEAYVTENVELVRNGASSNLRKQIENAISYGAPVVVAINKFATDSDKEIECIKEEAIKAGAFDAVESDHWAKGGAGAIDLAKSVVRATKEAPVDDKTPPNFLYSLEGSLEDRLRTIATKMYGAKDIELSELAKKQIEDYERQGFGKLPVCIAKTQYSLSHDPTLKGVPTDFIFPIREVRISAGAGYLYALAAKIMTIPGLSTNAGFMNVEVNDEGEIEGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT65</accession>
    <name>A0A1E3NT65_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_86566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019866.1">
        <property type="nucleotide sequence ID" value="XM_019165021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT65"/>
    <dbReference type="GeneID" id="30181708"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="185" mass="19983" checksum="0FE531FB19790EBE" modified="2017-01-18" version="1">MQIGSVSVSSEAIAAYNVYKLRVWCACISKQRLFPETDLLGDTKGRCSDRLATANGYLERANRQRRTGQHECTHTSRNGGPTKDWQRKREASIAGHQPSDNRVSHRHQQRSAPAINTIPVINNNTAAAPAAAWAGPPPPSPRFFFLCRVPACAPACGDVTQGSGARGHGGLDRLSGATLADWPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT70</accession>
    <name>A0A1E3NT70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14800</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020448.1">
        <property type="nucleotide sequence ID" value="XM_019160538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT70"/>
    <dbReference type="STRING" id="763406.A0A1E3NT70"/>
    <dbReference type="GeneID" id="30177225"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133507" description="ML-like domain-containing protein" evidence="9">
        <location>
            <begin position="22"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="655"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="693"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ49335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="725" mass="81442" checksum="329E6402B9C7881F" modified="2017-01-18" version="1">MVVIRFLLSLVLLLQLHSADAYKFLRSNSLLTCMESSQFTSTFFDVYYYPHNSTAVFYIDGSSSISGKIKAEFSLVVYGLNVYQDSIDLCTLGYKTICPISSGHIDVQGTYAVETDLTSKIPSVAYTIPDIDAYVKVLVYSTDDNNNTSPLACLKAVVTNGKTVQTRYAGWPIACVSGFGLVVSSVVSILGHSATASHIASNAVSLFIYFQNLAIMAMMGVARVPPIAASWAQNFMWSLGIINADFMQDIIYWYVQSTGGDVTAVLTNKDIISISIQKAKSLLFKREISAAPASDDYINDSSMYYTDEYNLSSKTLVLRGIQRVAYLSDIEISNIFMTGVIFFLFVGFVLVFLISVFRGFCEILIRSGAIKEHRFAYFRTNWSLVIKGSVYRYVDITFSTIVLLCIWEFTQNNSRGCLAFAIIIFAVFMILMGVGTIRIIMEGLKSVSQFKNPAYLLYGNEKFYFKYGFLYSQYLAKRYYWVGIHLFYLFIRALLIAVLQEKGKVCACLIFAIEIIHTVVIIYFRPYMDKRTNVFNILIAVINLINSVFFLFYSNVFRQPEVVSSVAAVVYFVLNAIFALVLLIFTIVTCTISLIHKNPDVQYEQVKDDRAAFMPNENMASQNEEMELAALGATAMQGQDRNSYIFGSNNEKPRLSPYGIDNTDSKAHLEPYDPDYSNDSGLEETKPNKLNPFANTEDTISVSSNHGSSSYDVENPFNNKARYHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTF0</accession>
    <name>A0A1E3NTF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-aminobutyrate aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70930</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="3"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020494.1">
        <property type="nucleotide sequence ID" value="XM_019163772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTF0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTF0"/>
    <dbReference type="GeneID" id="30180459"/>
    <dbReference type="OrthoDB" id="10260828at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000073">
        <property type="entry name" value="Probable 4-aminobutyrate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="471" mass="52302" checksum="65928444EE52993A" modified="2017-01-18" version="1">MSYTSKLFPEEPAAPSVVTESIPGPKGQEAIKTLGDVFDNRCTYFVADYEKSTGNYIIDVDGNVLLDAYCQVASIALGYNNPNLIKAARSDKMIRAIVDRPALGNFPGADFETLQKDLLKHAPKGQTHVWNGLSGADANELAFKAVFFNYQSKKRGFSKNFSAEEEETCMLNQAPGNPEIAILSFKKAFHGRLFASGSVTRSKPVHKMDMPSFEWPTAEFPAYKYPLEANAEANKQEDARCLAIVEDRLQNWKCPIAGVIVEPVQSEGGDNHASAVFFQGLRDLTLKYGAKLIIDEVQAGCGGTGIMWDHERYNITPPPDLVTFSKKMQSAGYWFHDPEFIPNMPYRQFNTWCGDPKGIIMAAAICDEIKDNNLLEPLRETGLYFYNQLEALQTKYPQFITNLRGKGKACFLAFDMPSGEKRAEFLGKMRLAGINVGGCAEVGVRLRPGLIFENKHVDIFIAAMEKVLPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTJ6</accession>
    <name>A0A1E3NTJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_89958</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020111.1">
        <property type="nucleotide sequence ID" value="XM_019165054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTJ6"/>
    <dbReference type="GeneID" id="30181741"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="169" mass="18005" checksum="E7E8B8CE5DADAF46" modified="2017-01-18" version="1">MDACGKPGTGRAPRAQSPTEIQALGSGVGQTLLAPLQPSERRQGQPGEFRETQWQLPQTRTQTRIRLFCTGRSRSPKAQHTGPNTGRGVGVRVGASLQRPLAPPPYCGRRNPFPVRPAASASSASPSAFSSGCRLQTAGWGLRAATGSAYLCNSRPLNLGSRALRSDWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTK7</accession>
    <name>A0A1E3NTK7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_14839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020483.1">
        <property type="nucleotide sequence ID" value="XM_019160560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTK7"/>
    <dbReference type="GeneID" id="30177247"/>
    <dbReference type="OrthoDB" id="202195at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="72" mass="8257" checksum="9B797A25970DA56B" modified="2017-01-18" version="1">MRATYPRMNSAKNASLFKVPPELYPLYAACFVVCCSATYFSFKKLTTDRTLKLGREIPQFDDKLEEALAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTU4</accession>
    <name>A0A1E3NTU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_70672</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with G-actin; ADP-actin form.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020211.1">
        <property type="nucleotide sequence ID" value="XM_019163702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTU4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTU4"/>
    <dbReference type="GeneID" id="30180389"/>
    <dbReference type="OrthoDB" id="10006997at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11285">
        <property type="entry name" value="ADF_Twf-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028458">
        <property type="entry name" value="Twinfilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759:SF1">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ADF-H" evidence="9">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="ADF-H" evidence="9">
        <location>
            <begin position="177"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="300"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="337" mass="35769" checksum="F881BD46FDF73988" modified="2017-01-18" version="1">MSSQSGIVPSAEVVVQVHDFVASEGRALTLTIDASSLSVDVGATTKATADLDADLDAIASQQLSDVECQYLLLRYGNGGKLAFISYVPDNASVRYKMLYASSKNTLLRSLGGELFDPILFVNSADELTAAGWRKIVHSMDGAVPLTESEQNLRDVREKELYLNSSKASHSGQLVSDSSNALLFQIDPALEPLLAAGSASPASLISLEISEEVLKLIDSADSVAVEDLVDRVQPISGPSYHLYTADTGACFFILTCPSGSKVRERMLYAANKQGLLNHLKSNGWEFSKVVEAGDADEIELSELQTAAGGDSGATAPDTAGPGGSARLRFAKPKGPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTV8</accession>
    <name>A0A1E3NTV8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_101487</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020539.1">
        <property type="nucleotide sequence ID" value="XM_019159637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTV8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTV8"/>
    <dbReference type="GeneID" id="30176324"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133519" evidence="2">
        <location>
            <begin position="23"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="192" mass="20456" checksum="43D062966CE3E47E" modified="2017-01-18" version="1">MRLSNIGLLVTTLISNIGISNADTDNELEKRNSCSTQVSSCSLTPYNHGYPAASVSWGNTVTDLGNGIYEGTMNFQGKDCSQFDNTNEIKIDGGAATVVVYSYNGYSCSSANPYYSQCVQGTSSPTTTPSGSSSTTPSTTPSGSSSTTKPSTAPSDHHPPLSHRLLHLDHHPPLSHRLLHLAHRLPHALPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTY4</accession>
    <name>A0A1E3NTY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin-activating enzyme E1 1</fullName>
            <ecNumber evidence="6">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_29501</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="6"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020251.1">
        <property type="nucleotide sequence ID" value="XM_019162594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTY4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTY4"/>
    <dbReference type="GeneID" id="30179281"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.180:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018074">
        <property type="entry name" value="UBQ-activ_enz_E1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00536">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="15">
        <location>
            <begin position="889"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ49138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1018" mass="113911" checksum="956E3FDF81E74BD8" modified="2017-01-18" version="1">MQIDSPAPEDKIDEGLYSRQLYVLGKEAMLKMASSNVLIIGLKGLGLEIAKNVALAGVKSLSLYDPEPVVLQDLSSQFFLAESDIGTQRANATLPKLAELNQYVPINVVDALTPEVIKTFSVVVATNLPLSRQLELNELTHANNIRFISADVRGLFAQAFVDFGDSFAIFDQTGEEPKTGIVSDIEKDGTVTMLDDNRHGLEDGNFVKFSEVKGIEKLNDGSIYKIKVLGPFAFQLVDFDPAWGTYQKGGLYTEVKTPIKVSYQDLATQLRTPDYFMSDFAKFDRPPQLHLGFQALHEFQIENNGQLPGPHNEEDATTIVNLTKKLAAKYPDILAGADVDEDLIRELSYGARGDLPAMVAFYGGFLAQEVLKACSGKFVPIKQWLYFDSLESLPESDEYKRSEEACKPINSRYDNQIAVFGLNFQRKLANLKIFLVGSGAIGCEMLKNWAMMGIATGPDGKIFITDNDTIEKSNLNRQFLFRAKDVGRNKSEVSSNAVCAMNPDLKGKIEASIDKVGEDTEHIFNNDFWENLDFVTNALDNVEARTYVDRRCVFFRKPLLESGTLGTKGNTQVVIPNLTESYSSSRDPPEKSIPLCTLRSFPNKIDHTIAWAKSLFQGFFHDAPENVNLYLSQPNFVETVLKQSGDVKGILETINQYLTTDKPYTFEDCIKWARLQFEDKFSNDIKQLLYNFPKDAVTSKGEPFWTSPKRAPEPLVFDINDPNHFDFVTGGANLLAYVYGLKGDNGEPDKKYYTSVLSKIDIPEWNPKSDVKIQVNDNDPDPNAGLVTDNSVIEKLSSSLPSPSSLAGYRLSTVEFEKDDDTNHHIEFINAASNCRAENYFIEVADKSKTKFIAGRIIPAIATTTALVTGLVCLELYKVTDGKTNIEKYKNGFVNLALPFFAFSEPVASAKGKYGDKEYDKIWDRFDLYGDLTLQEIIDYFKKNEGLELSMMSYGVSLLFASFYVKKMEPRYKMKISEIIELVTKKPLTENDKSLILEVCLEDENGEEPDVPYAVLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NU39</accession>
    <name>A0A1E3NU39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_28845</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell wall beta(1-&gt;6) glucan synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRE9/KNH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020673.1">
        <property type="nucleotide sequence ID" value="XM_019162559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NU39"/>
    <dbReference type="STRING" id="763406.A0A1E3NU39"/>
    <dbReference type="GeneID" id="30179246"/>
    <dbReference type="OrthoDB" id="2432613at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045328">
        <property type="entry name" value="Kre9/Knh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008659">
        <property type="entry name" value="Kre9/Knh1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154:SF1">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05390">
        <property type="entry name" value="Kre9_KNH1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133499" evidence="5">
        <location>
            <begin position="24"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="7">
        <location>
            <begin position="29"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1 C-terminal" evidence="6">
        <location>
            <begin position="166"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="283" mass="30708" checksum="88CD63F10452597F" modified="2017-01-18" version="1">MANIHSFFCSFFILLLSLRSCLGQLVIDSPSAGSTFDVVGGEVTIPVKWEDDGNDPTDDQITSYAFKLCTGPNQPIEGLQLIKGITPSQVKGNSYDAVFDADNGADGSYFVQIYCSLSNGGYMIRYSDRIQLSGMSGSYKPSGSGPSPDGETNSDDNGQQNDADISKSFTVPYTLQTGKTRFAPMQMQPGSEITVSSWSRRFPSSAVTFYSSFASQPKIISTRTPGWSYTMSSFVNYATPAPFPSVVGWYAPSKRLQSASLDAPMPGASADAKRKLQKRRWVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUP1</accession>
    <name>A0A1E3NUP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14826</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020471.1">
        <property type="nucleotide sequence ID" value="XM_019160552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NUP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NUP1"/>
    <dbReference type="GeneID" id="30177239"/>
    <dbReference type="OrthoDB" id="3997979at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="80"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="3">
        <location>
            <begin position="657"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="489"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="83568" checksum="E703C2B757C4BDC7" modified="2017-01-18" version="1">MSQVTVPATVPLDAQQTNGEAVDGEEVTLLEDVLPSFEMHNYMFNRTIYDTENISSSGNPPSYEDMNVPSTTSNIAPRDLNNFVDPTKNPNLLLLNNLEKFQKIDLPLTVQVVLTKTVPRLGSKQERENPLRQYRPGEVVCGYITIENKSDDAIPFEMFLVSLEGELTIPHPHKPEELIKKKFLKTYDLSACFHYGCIDLASQGVSLQTRMDELDKSLIGFDNDRVITPKIKHKKFFAFKLPHYLLDTSCFDQLPSHLKLPPSFGVDKSAFKGLAKYIKVDPFLGYGRLDRYGSPIKTNDYAADGQSVSYYISVQFIGRKLDFYKKFYTNDTKHEYDFIFLKNVEYNFRVDTSSNTDEYSADDSLSDISTTQQLKMTENMITDKLHEIVERKNLKKIGITDPRKQDEIIFSNMSNNKKSQQLYRAELVPLLSDTKTSMSDGEGFSRVKTVKLVKDFFSKVEGDMTVRLSMNRNSQLRAIKPKQLKHASSKPSSKSNSSLSLNTQNQLNSNSSSFTSLRSLTLSPTTSSDSVSGLRPVTSAPERLYIYLSFKNPDLKRNTKPNLPSTITFSPSLRVYNIQSPYPIPVTFDNKYLFNGGLEPENLQNLKKKFSFYYQELVDVLKDLDSGLARSMYNKVNGLAKLHVSEQVVKRLFETQTIDLHNQWKFNKERNIYECEFGIPLVFDSKNMEKISSYCVVPGFQQCLLSRLYAVDVEVSIKKARAKMNMKFPVHVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDG1</accession>
    <name>A0A1E3NDG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_184953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015267.1">
        <property type="nucleotide sequence ID" value="XM_019162025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDG1"/>
    <dbReference type="STRING" id="763406.A0A1E3NDG1"/>
    <dbReference type="GeneID" id="30178712"/>
    <dbReference type="OrthoDB" id="2109411at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF9">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="209"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="211"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="265" mass="30744" checksum="9EAFFC1DD4D04602" modified="2017-01-18" version="1">MEDLTAQPSNIESTVLPLLSICSLTGEYQEKKVGRKRLKYRKLVDIEELPAAKSVQLVQLIQVCQDTFEKEADVLIDNQMAEEAVDELFLNYKQYLIGLFSLSTQYYEPSKQEYNNYEDTWFSGVDLNLDNTSSSFDTTDSFTFDLNDFDFDYANLTTSTNSEISQSFDFSQSQLCNGPDFLFSDYIQFHDETKENDVKADDYYYSGSRRRITKESRNMLEALFIVKNSPNSAERKLIAERCHLSPAQVRIWFTNKRSRCKRKGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDI4</accession>
    <name>A0A1E3NDI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_74775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015279.1">
        <property type="nucleotide sequence ID" value="XM_019164865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDI4"/>
    <dbReference type="STRING" id="763406.A0A1E3NDI4"/>
    <dbReference type="GeneID" id="30181552"/>
    <dbReference type="OrthoDB" id="9970124at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43123:SF3">
        <property type="entry name" value="NODB HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43123">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="81"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="338" mass="38715" checksum="0BF77DF2C00EF9F4" modified="2017-01-18" version="1">MDTIGYVPGPMPKYEPERDLLGYGENPVDVEWPNGAKIAISFILNYEEGGEHNILHGDDRSEAYIWEKSGANTEVLNGRHLMSESEFEYGSRSGCWRLIRLFKEFGWPMTLYAVGTAFQKNPEFARACVRDGHEIANHGLRWVNWAGLDDKTQIEMIKQAILTLEEASGEAPVGAYIGRGNIKTPELVKIAHEQLGYPFLWESDCYNDDIPYWRDAEHEKDLPQEEKKGLLMIPYNYVNNDYKFVASSPGPGFPGAFVYEDYLKNEFDQLYREGGRMMNIPMHSRILGKPGRANALRNFMKYISEKEGVWVCNRRDIALHFKKKFPYVPGKLAVKKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDP6</accession>
    <name>A0A1E3NDP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_37561</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015355.1">
        <property type="nucleotide sequence ID" value="XM_019163088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDP6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDP6"/>
    <dbReference type="GeneID" id="30179775"/>
    <dbReference type="OrthoDB" id="14446at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052373">
        <property type="entry name" value="Gamma-glu_amide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="3">
        <location>
            <begin position="2"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="317"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="356" mass="39948" checksum="ACEF337679BE3883" modified="2017-01-18" version="1">MCRFLIYKGKEPMTLSNLLTKPAHSIINQSYDSRLRIDAQPVNGDGFGVGYYTLDDDQESGDSSSSSSSKDNSPCLYKAITPAWNNLNLQRISEKTRSKLIFAHVRASTYGVLSETNCHPFTYKNIMFMHNGGISNFNKIKLRLINFIDEEYFLQIKGSTDSECCFALLLHCLKQNGFDPDSGDVCDLTVLRESLVDTIQHIKNWVKEVSNDPSLLNFAVTDGENIVVSRYVTSKTDEAASLYFSTGSSFVEFAPGRFKMERMNKSQNLVMVASEPLTFERNDWISVPTNSCISIRNLNVLLHPIVDEYYSKDPVQPRSNQFASSKGMLGSYPAADDQYNNAVPPLEREGRAVRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDW0</accession>
    <name>A0A1E3NDW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_21585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015438.1">
        <property type="nucleotide sequence ID" value="XM_019162211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDW0"/>
    <dbReference type="GeneID" id="30178898"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="646" mass="74232" checksum="BEFC7FAB525E5CC8" modified="2017-01-18" version="1" fragment="single">MFKVFKTTGIEGNYNISANPTFVSNNCWAIYPAKHKTTKKAYSVWQFQKKDWETRLTNDGIITKNNKKLVLNDIYENIRLFIGNQAKLKHPNFLTVIEPLEDHKNRILFVTEYVVSDLNNTDKSDLDEIMITKGLLQVSNGLKFLHDSVQSVDLNINPSSIVITENCDWKICGLPFLENIANGIAEKFVDPIDSRLPSFLSIDFRFSSPNLLLKHKVDYINDLFSLCCVIYFLFNDGEYMIKCHQSSGLTDYERQIKKLNQVLGSIQTNSTGNKHAFFNKIPDNYYITFIDILQNSQESNTDVIQLKKIITVNDLIESNIFNNELIRILNIIDEFETLPVTEKITFLKKLKLEIEKFPKPLLINKFIPILINAVDMDQVKKNVQPTPEMEELIIESCDNLVILSQNLSQLTFTDKVFPFILILLKRLQFDGFKILLLKNLPIIQKCLNASIDNNSSNDSFQKFSLDLFDKCINEKNSLAVQELTLVNLKTLLPFQPYSVITSDILPRICTIYSTTTSLKIKNLTLASFITMIVEVKNNSLDDFLVVDKILPLIYNTSSANYANTKFTNNILKLYNCIFDKLSKSTNKSFNSQGNETEIYDIIMEIGFHVWKIAKLITNRQDLNVAYGTWSKVENFLKKDLDSKVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE44</accession>
    <name>A0A1E3NE44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphinganine-1-phosphate aldolase</fullName>
            <ecNumber evidence="14">4.1.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Sphingosine-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_37263</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16 17">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.27" evidence="14"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015479.1">
        <property type="nucleotide sequence ID" value="XM_019163068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE44"/>
    <dbReference type="STRING" id="763406.A0A1E3NE44"/>
    <dbReference type="GeneID" id="30179755"/>
    <dbReference type="OrthoDB" id="10254570at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008117">
        <property type="term" value="F:sphinganine-1-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000020">
        <property type="entry name" value="sphingosine-1-phosphate lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 17" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="16">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ44366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="485" mass="52829" checksum="F017E82AF497BF91" modified="2017-01-18" version="1">MLPPVQKRVKQEVADARRLLENDLIVKAEHVEHHQLPEEGLSSDAVMAQLDALDSIKAVAWKEGKISGAVYHGGDDLINLQTESYHKFAVANQLHPNAFPGVRQMEAEVVSMVLGLFNAPSTGCGTTSSGGTESLLLACLAAREKAYAEKGITKPEIVAPKSIHAAVFKAAKYFNMTLRLADLDPKTFTADISHVKRLVTSNTAIILGSAPNFPYGTIDNIPELSKIAVKHNIPLHVDCCLGSFVIAFYEKAMGKKLPFGFDFRVPGVTSISCDTHKYGFAPKGSSIIMYRSPEYRKYQYYITSEWVGGLYGSPTLAGSRPGALTVGAWTTMIFLGQEGYVKNIRSILETSQILRHAIENEIPELDLIGDPLLSVVAFKSDVIDVHVLGDLLTKRHGWHLSSLQQPAALHLAVTKLTGQAILQLVEDLKKCVEECVAAVADNRQAIKSDTAQLYGVAGSLSTRGVVDQLVGVFLDVLYENDKTMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEG6</accession>
    <name>A0A1E3NEG6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_37060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015622.1">
        <property type="nucleotide sequence ID" value="XM_019163052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEG6"/>
    <dbReference type="GeneID" id="30179739"/>
    <dbReference type="OrthoDB" id="3991365at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020266">
        <property type="entry name" value="Tom6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17112">
        <property type="entry name" value="Tom6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="52" mass="5524" checksum="A9313B3E9F29BC90" modified="2017-01-18" version="1">MNGFAMPQDKPASEPGFFSSPLGKIGLNVVLFVAGVALIQSPIMDMLVPQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEJ4</accession>
    <name>A0A1E3NEJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_13220</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015652.1">
        <property type="nucleotide sequence ID" value="XM_019160029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEJ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NEJ4"/>
    <dbReference type="GeneID" id="30176716"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="483"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="11">
        <location>
            <begin position="90"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ44539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="590" mass="64758" checksum="2BAD6C5A5BA6411C" modified="2017-01-18" version="1">MSIEKTYDEKDMVDTSVSPVQDYSSKQGDANGYVDPYAQFEKEDNGFFRNFIDGFKPLDFGEIDPNLSDVERANLIAARAPLKRSLKNRHLQMIAIGGSIGTGLFVGSGSALATGGPAALVIAWILTGIMMYCTVQALGELCVAFPVAGSFVQYNTRFISASWGFCMAWNYALQWLVVLPLELVAAAITIDYWNAEINSAAWVTIFYFLVLFINVFGVKGYGEAEFIFSFLKVLAIVGYIILGIVINCGGGPEGGYIGGQFWKNPGAFAAGFKGVCAVFVTSAFSFAGTELCGLAAAETENPRKSLPSATKQVFWRIALFYIISLTLVGLNVPYTDPRLLGNSGAAASPFVISIRNAGIKGLPSVMNAVIMIAVCSVANSSVYGASRTIASLADQGFAPRIFGYIDREGRPLVGVAISMIIGLLCYLAASSHHADVFNWMLALSGLSSIFTWGSICACHLRFRRALQVQGRGTDELSFTSQCGIIGSCFGVLLNCLILMAQFWVALFPIGEAANPTSFFQVFLCVPILIVWYFFWVFYKKDYKFFLRSKDIDIDTGRREVDLELLKQEIAEEKAFIRSKPFYYRVYKFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEQ8</accession>
    <name>A0A1E3NEQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_74993</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454010">
        <property type="protein sequence ID" value="ODQ44048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015161.1">
        <property type="nucleotide sequence ID" value="XM_019164933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NEQ8"/>
    <dbReference type="GeneID" id="30181620"/>
    <dbReference type="OrthoDB" id="3994150at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="553"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="132"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ44048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="606" mass="68899" checksum="A435488DC2B70B93" modified="2017-01-18" version="1">MEKPAENHSNDTDSIDSDNQDVQKFNPLNDPEDQGNAENLSHHVSRILSTQEGMERVATLARVLSTKTKKDMDHFEINKENFDLELLLKYLRDRSAEQGIESANAGVSFRDLTCWGVDASAAHGPSVTEMSINYLSFWKMFQKDNRKQRMIIQNFVGVLEPGEMVLCLGKPGAGCSSLLKAVAGEIENFTKVEGSFSYDGLDQAEMMQKYKGYVVYNPELDFHFPYITVKETIQLALRCKTPQKRIDNMSRSEYVDNMLRVSFLQTTFFFSHSILTFVFDFLLQLWCTVFGLTHTYATRVGNDFVRGVSGGERKRVSIVEALALNASVYCFDNATRGLDASTALEFTQCLRTATNMLGCSAFVAIYQAGQNIYELFDKVSVLYKGRQIYWGPADQGVSYFEKMGYLKPSRMTAPEFLTAVTSPSSRQVQPGFEGKVPESSEEFAEYWVNSPEYANLMTEMDEFDANHNGDETRQRLDFVNQQRKQKGNRQKSQFMVTYWSQLYYLMLRGFQRTKGNITYTIVYISSFVTKGFVVGSMYYHIPKSTNGAYSRGGILFYVLLFCSVTSLAEIANSFSTRNILVKQKSYSMYHLSAEALQEIITEFPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEV3</accession>
    <name>A0A1E3NEV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GABA-specific permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_74936</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015211.1">
        <property type="nucleotide sequence ID" value="XM_019164922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEV3"/>
    <dbReference type="GeneID" id="30181609"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="59843" checksum="BA8EA09B644D68D8" modified="2017-01-18" version="1">MAEKLAAKTSGFSPITSAQSRIHDIAATASHLRAIDAKVVDNDDDLLAEIGYKQELKRRFSTFQIFGIAYSIMGILPSVSSLLGTALSAGPAGAVWSWAVASIFILTIGVSMSELASSIPTSGGLYYWTFYYAPEAWRVPLSFVIGLSNTMALCSGSVSVFYGNAEEILAAVYLTKDGNFDITTGKTYGVFAGCVITGAVVTCLSSKNVAMLQSISSICHTGLLVLFFIALPIGTRVNRHEFNSRGFIFGEVQNYSDWPIGWQFCLSFMTAVWTIGAFDSCVHMSEEAKNASYGVPIGICGSITACGFLGFFILICTTACMNPDIGTVLATDTGFPMAQVIYDSLGRRWAIAFMSLMAGCQWLMGSSLLTAASRQIWAFARDDGLPFCSIVKVVNKKLRVPIRAVILACLISLAIGCLCLAGTAAANALFSLGVLGNYLAWCTPVFLKLTFGKHKFNPGSFYLGPVFSPIVGWIACAWGAFITVLVCFPATKVVTKENMNYCIVITGGVWILSIVFFFTYKYKYYHGPKSNLDDDNIEVVSSAEDVSYPQDEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEV4</accession>
    <name>A0A1E3NEV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phytanoyl-CoA dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74365</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PhyH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015767.1">
        <property type="nucleotide sequence ID" value="XM_019164751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEV4"/>
    <dbReference type="STRING" id="763406.A0A1E3NEV4"/>
    <dbReference type="GeneID" id="30181438"/>
    <dbReference type="OrthoDB" id="445007at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883:SF45">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33477" checksum="7E572702FEBC6BA0" modified="2017-01-18" version="1">MIQRIDVKEPVESFVKALEQDGLVVVRNFASADDLVQSLQEVQPYLDADKPWEGKLFPPETKRCFRLIGRSKTVREKFFSHPLYQQVSEHFLARTTTNYYDDTPTTYTTHPLLSMSMTMAIGPGAKAQRIHRDEKNHHATHVKSETYVMGNDLLVGLMVPTCATTIENGATQVIPGSHLWGDHRPPYRDELVSAELDRGDAVLLLGSLYHCGANNYSTQIRPMHIMFQCPGVYRQEEIPWLSYDVQDVKTYSPLVQQRLGYVSSAPNLGWVDLKSPDFLLREEGDEVGHKDLDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF79</accession>
    <name>A0A1E3NF79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_74478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015895.1">
        <property type="nucleotide sequence ID" value="XM_019164780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF79"/>
    <dbReference type="GeneID" id="30181467"/>
    <dbReference type="OrthoDB" id="3998204at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="2">
        <location>
            <begin position="379"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="530" mass="60901" checksum="71F97AA38BB7F234" modified="2017-01-18" version="1">MMFKDTSNNTSSEYAPGETSVLSDLDHPEYLSHSVGDRYRAEKAEAFLWNVVENPVDLDPIISGEGMLASDDSEMQNWITSTSEASSVSPSSYFAVMNGTDMFESHLFGHFDPYDQETVNLPEHRATLTVDDMDFLEVTFDEAHEFIKSEEQQRLHLPTPPLSESSELRGSSQLEETHSKAKSLEVRPEAGEAKVEIKDEILVEEDEEEQRSEMIMPKKGKRTVVLYPNEILERARKYTNTVQEANRNTTKIKGRDIRLLQQSAAAGRVSIGSSVYTIVPESEWKINYMLYLIPNGYVSSVFLVSFLKSMVDDVWVYYDNINEQPHFALTNAKLPFRYCNQVANYYEPFIIREKRKDGRILTKEEKRARSDRRSRGKSKDDSLFHIEALCPYCPIKFDNVNKFDDYFYGRNDSDYLHHVTKHHGVYSDGSEHALPDLVLLDGRGRFYAHCEECEELVKIKDPTGEEIVGNRFLGYLRHCLKHRNRCKNGSGESRLRRRERVVENAARRNLLYEDGDKGELLVHSRIGAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFG0</accession>
    <name>A0A1E3NFG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleoporin Nup82</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74569</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015431.1">
        <property type="nucleotide sequence ID" value="XM_019164806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFG0"/>
    <dbReference type="STRING" id="763406.A0A1E3NFG0"/>
    <dbReference type="GeneID" id="30181493"/>
    <dbReference type="OrthoDB" id="341482at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="84870" checksum="C665DD9098A99349" modified="2017-01-18" version="1">MNSFFKNSIINHSKEFTSSEFYRTVTRKNGSEIITVTGGKITLTQLNTDKSPQIAIAEGLPFEPIEILMNECEQLLCAYDQTNCWLFSLNKEGTSIRFTVTYKVKLDLAENETVLQVVFNNISKYQSEIVVLTTTEIRAYNTNLSLKSPVIRYNFKKEYEKNISSNTYDISDPIIDPVSICFASCPPNSVFEFEDAGYFSGNTPQNDLTLFLLTSDASVYQIFPFFPYELSASKEWLGDLFDSTSILFKSKSDSIEQFEMMASVKVSALLSKASNPASIEISDALPSIYRKGKITGPLSMESFPEDLYVSEAIKLLSLPNDLLIIVFNHTVVVFNRNSKATMIFEDQKIETRDSFLLLDSIIFNKNEGDVFTAMVHPVTRDSVFVVSSNGSLIQVDFSKWIEPLTAGLNTGDLSEFNKLCQTGNLPTEVITLGKMHLSKEKPLTSRLTLRTHENSIWFSWNTRDVYAMVIKANTLDILSMFLVSTSKASTTEAADFAETQNEKISGNEKDKKSYKSLLLGNYEKDVMPLIKGSLAKITEINSAMQKFPSTILNEQNTTASELKTVHELSELATSGQLILFRILSIITDRLRLMTNEYHNQINTYHQIMLQKEAVVGKFLRLKGVYLDSIERQKKLNARMVNVMSSVEKLESTKNMTNVSISYQENAYFKELARIRDFTVKKESELEEVEFLLTNIKNAEKSVLTENKEVILKNFESRRILEKLKLQLETQDKFVEYLVSLLRNFNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFJ5</accession>
    <name>A0A1E3NFJ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_64537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015979.1">
        <property type="nucleotide sequence ID" value="XM_019163481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFJ5"/>
    <dbReference type="GeneID" id="30180168"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="105" mass="11955" checksum="7E8C456E89BB6314" modified="2017-01-18" version="1">MCYRSEGTFSGDDTFKLLVVALWSTAVYIFKHYSLISKQNTGKTGVRIIGVFRPVFPELVEEITLRSMIKTAAASIRFTSNKERSPTKDRKHLGFTQTIEDSLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG09</accession>
    <name>A0A1E3NG09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ras-related protein RSR1</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_164565</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016165.1">
        <property type="nucleotide sequence ID" value="XM_019161213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG09"/>
    <dbReference type="STRING" id="763406.A0A1E3NG09"/>
    <dbReference type="GeneID" id="30177900"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007120">
        <property type="term" value="P:axial cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000755">
        <property type="term" value="P:cytogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032507">
        <property type="term" value="P:maintenance of protein location in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000114">
        <property type="term" value="P:regulation of establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04177">
        <property type="entry name" value="RSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000631">
        <property type="entry name" value="Ras small monomeric GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041841">
        <property type="entry name" value="Rsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="173"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="190"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ45052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="265" mass="29486" checksum="9E12B57C8E8551D6" modified="2017-01-18" version="1">MRDYKLVVLGAGGVGKSCLTVQFVQGIYIDTYDPTIEDSYSKEIEVDGRACNLEILDTAGVAQFTAMRELYIKNGQGFLLVYSVTDKKSLQELLSIREQIIRIKGTSNIPMVLVGNKCDLAEERELEPEDGIDVSKQWNKVPFYETSAMYRMNVDDAFVDVVRQIIRKEVQQQQVTEDHQQQQQPQQQQLPSTAGSSALPKQNGLSSNKQNQSSAPGSATQSSRTPQNPQNPQKNPQQQRAALAAQNARSAPPKKAKKSRKCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG46</accession>
    <name>A0A1E3NG46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015651.1">
        <property type="nucleotide sequence ID" value="XM_019161831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG46"/>
    <dbReference type="STRING" id="763406.A0A1E3NG46"/>
    <dbReference type="GeneID" id="30178518"/>
    <dbReference type="OrthoDB" id="2014058at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008699">
        <property type="entry name" value="NDUFB8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12840:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12840">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE ASHI SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05821">
        <property type="entry name" value="NDUF_B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18551" checksum="5DC8EA9E9A6E183D" modified="2017-01-18" version="1">MFSRSILTKSSLLRVQAFKRFNSTSAKYGLPKFLPDAEKQFGIKTPENYVPPQYRDPYAKYDDQQNRVNFGEEIHPYHDYMDMWSPDHFQTVKDHVAVRELSIFFGLLGLFAYSIYAFDIWPERPNMPRSYPYEGLYKDLGGREGEQSLMQARVDKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG49</accession>
    <name>A0A1E3NG49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fatty acid synthase subunit beta</fullName>
            <ecNumber evidence="21">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="21">3-hydroxyacyl-[acyl-carrier-protein] dehydratase</fullName>
                <ecNumber evidence="21">4.2.1.59</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
                <ecNumber evidence="21">1.3.1.9</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">[Acyl-carrier-protein] acetyltransferase</fullName>
                <ecNumber evidence="21">2.3.1.38</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">[Acyl-carrier-protein] malonyltransferase</fullName>
                <ecNumber evidence="21">2.3.1.39</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">S-acyl fatty acid synthase thioesterase</fullName>
                <ecNumber evidence="21">3.1.2.14</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PICMEDRAFT_73908</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20 21">Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 21">
            <text>(9Z)-octadecenoyl-[ACP] + H2O = (9Z)-octadecenoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15057"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9924"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78783"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 21">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 21">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 21">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14 21">[Alpha(6)beta(6)] hexamers of two multifunctional subunits (alpha and beta).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the fungal fatty acid synthetase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="21"/>
    <dbReference type="EC" id="4.2.1.59" evidence="21"/>
    <dbReference type="EC" id="1.3.1.9" evidence="21"/>
    <dbReference type="EC" id="2.3.1.38" evidence="21"/>
    <dbReference type="EC" id="2.3.1.39" evidence="21"/>
    <dbReference type="EC" id="3.1.2.14" evidence="21"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016238.1">
        <property type="nucleotide sequence ID" value="XM_019164617.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NG49"/>
    <dbReference type="GeneID" id="30181304"/>
    <dbReference type="OrthoDB" id="5417908at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004313">
        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016409">
        <property type="term" value="F:palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03447">
        <property type="entry name" value="FAS_MaoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000017">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000078">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.100:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000006">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000007">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000169">
        <property type="entry name" value="Fatty acid synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2430:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000003">
        <property type="entry name" value="Fatty acid synthase subunit beta dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="FAS1-like_DH_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016452">
        <property type="entry name" value="Fas1/AflB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013565">
        <property type="entry name" value="Fas1/AflB-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041099">
        <property type="entry name" value="FAS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040883">
        <property type="entry name" value="FAS_meander"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003965">
        <property type="entry name" value="Fatty_acid_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08354">
        <property type="entry name" value="Fas1-AflB-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="FAS1_DH_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22235">
        <property type="entry name" value="FAS1_thioest_ins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17951">
        <property type="entry name" value="FAS_meander"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17828">
        <property type="entry name" value="FAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005562">
        <property type="entry name" value="FAS_yeast_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01483">
        <property type="entry name" value="FASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 21" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 21" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 21" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 21" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9 21" id="KW-0520">NAD</keyword>
    <keyword evidence="7 21" id="KW-0521">NADP</keyword>
    <keyword evidence="8 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="23">
        <location>
            <begin position="1670"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="active site" description="For acetyltransferase activity" evidence="22">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="active site" description="For malonyltransferase activity" evidence="22">
        <location>
            <position position="1814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR005562-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ODQ45125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2056" mass="228118" checksum="B4B2EF41A5EFEC47" modified="2017-01-18" version="1">MTVTRPLSLSHGSLETTILIPSDSYLFYQQLTSDFKKTLPEPTEGFADDDEPSSKSELLTKFLGYVIESNSIEAEKELAVKLILDDFETRYLQSSDIHTFASQLLASEEYPTTLQKINSNLIKNYFAAKVYLKEDFTSSNVSSALLKAASDDKASIFAIFGGQGNTDDYFEELREIYTTYNGLISGFLSKVQSKMQELISSTSDIDRVYTDGFDFINWLKNPSQTPSTDNLLSIPVSCPLICVIQLCHYIITCKTLGVTPGQFKSCLKGTTGHSQGLVTSLVVASADSWESFDVEALKAVELLFYLSVRCLQAYPSTSLSPSMVRDSEENGEGKPGPMLSIRDLSVVEVENFVNQTNQHLPSEKHIKISLINGARNVVVSGPPESLYGLNLNLRNAKAPSGLDQSRIPYSQRRLKFSSRFLPIHSPFHSNLLAPATPRILEDFKKSGLAFDQSKMTIPVYDTFDGKDMREFSGSLAARVVECINTLTVNWEPATDFKATHLVDFGPGGVSGLGVLTHKNKEGTGARIIVAGVLDAAKSDAEYGFKQELFDKRTSALTYAQNWLDEFRPKLIKNKDGKVYVDTKFSRLLSRAPLMVPGMTPTTVSPSFVIAAINAGYNIEIAGGGYFSEPAMEAALQEVAENVTPGSGIGINLIYVNPRMLQWGIPMIKKMRERGFPIQSLTIGAGVPSLEVAGEYIETLGLTHLGLKPGSIDAISQCIAIAKAYPTFPIVVQWTGGRGGGHHSFEDFHQPILQMYARIRRCSNIILVAGSGFGSAEDTYPYLTGTWSRSFNYPEMPFDGFLFGSRVMAAKEALTSEEAKKLIASCPGVSDSQWEQTYKKPTGGILTVKSEMGEPIHKIATRAVKFWKEIDDTVLSLPKNKMLEALEKKRDYIISKLNADYQKPWFGKNSEGVCDVKDMTYEEIAQRCVELMYIKKLGKWIDPSLREFTGKFLRRIEERFTTQSTVSVLQSFTELDVPAPVLKAVFSKYPDAKTQLVNAEDADFFIASCLNPAQKPVPFVPILNDKFEFFFKKDSLWQSEHLEAVVDEDVQRTCILHGPVAAQFVKKVDEPIKDILDTIHEGHIEKLLKDVYNGDESKIPVVEYFSSKPAYKPSEVPGVTVSESANEITYKIGSTVPDAEKWYTLLASDEYNWRHAFISSNIIVQGYNHVSNPAKKVLAPAKNITVKITNFSDSSKSTISLYSIVKGKEREVVNISFDGSTINMKLIETRTADGAPVPLELFYSYVPSDGFAPISEVISGRNDRIKEFYWKVWFGMKEPIDFDFDVSSKIDGGSMQINSKDIAQLTHAVGNSCEYFVPRAGRDQLAPMDFAIVIGWKAIMKSIFPKCVDGDILKLVHLSNAYTILPNSAPLKVGDKVSSDAEIVSVVNGPTGKTVEVVGTIYRDGTPVMQVTSAFFYRGSYEDFENTFEKTEETPSQVTISTAKDAAVLKSKEWFHVEDNVELLNKSLIFRLKSVYKFKSANVYSHVSTTGNVYYESPTKEIKKAAEIVYNAAFSYGNPVIDYLTRNGTLIEQPIMFENPIPLARALETKAPISNETYAAVSGDFNPIHVNRIFAAYADLPGTITHGMYSSASVRSLVEMYAADAVAPRVIAYKANFTGMVLPNDELTTKLEHIGMINGRKIIKIETKKVETDDVVLSGEAEIEQPVSTFVFTGQGSQEQGMGMDLYGSSEVARSVWDKADKHLIETYGFSILDIVKNNPNELTVHFGGPKGRTIRENYISMMFETIAADGSLKSEKIFKDIDENTSEFTFKSPSGLLSATQFTQPALTLMEKAAFEDMKSKGLVPAESMFAGHSLGEYSALSSLADVMPIESLVDVVFYRGMTMQVAVPRDSLGRSNYGMCAVNPSRVSATFNDAALRFVVEHISKQTGWLLEIVNYNVENQQYVTAGDLRALDALTNTLNVFKLQKIDIVKLQEIMPLEKVAEHLSEIVDEVSKKSLAKPQPIELERGFACIPLRGISVPFHSSYLKSGVKPFKRFLEKKIPMSAVKPASLIGKYIPNLTAKPFQLTKEYFQDVYDLTGSEKIKEILDNWEHYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG77</accession>
    <name>A0A1E3NG77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation protein SDO1/SBDS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_73913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016247.1">
        <property type="nucleotide sequence ID" value="XM_019164619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG77"/>
    <dbReference type="STRING" id="763406.A0A1E3NG77"/>
    <dbReference type="GeneID" id="30181306"/>
    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="104" mass="12007" checksum="E78A4319546147DD" modified="2017-01-18" version="1">MTYTDINRSSEHVFYKGNKEDFLIFLDDVDEYQKYIAGDTSIPMSRFIAKFDIYRNATGNGSEGILEIASEQNLNEEFGDFKSVEDEIIPKILRSGKLQNMHKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGE2</accession>
    <name>A0A1E3NGE2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_60311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015751.1">
        <property type="nucleotide sequence ID" value="XM_019163428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGE2"/>
    <dbReference type="GeneID" id="30180115"/>
    <dbReference type="OrthoDB" id="10473532at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="474" mass="53446" checksum="D4594F1A4EE5E5B4" modified="2017-01-18" version="1">MSVVMEPDTRPRIYKSCLIRVGGFQDEHEEEDNMEEKTEEEGAPEQMDDIEEEAEEGEVDEYMHNVEANELVNSINPSFWELPIEEGAEPQPEPQEEQDPIKGKPKGTAKGKGRTREANGNSNTKAKSKKKEKESESEEKRVPVRVSQMINNKSKQNTGSSASIVKKKPATTYGKRKNNNFSFKAYVHKPPRYVQKQIDLAKEAGAQPVEEEEAEAEVEEEAEETVDSEFRTPEQQQQLPPLHNQPPAQMYPVSRQEQRIREELAEVQVARSSPNIDSPMDDRDVAHDVRTPKPGQFQHNVACILSSSAVVEPDEDNAGPEPSFSDEATKIEEPGNDPQHAEHADHGPPDLGKLNRTLVFNSFDTADDPIKSVLARIVHETEARVAAMERELDAEHEAVLRDVQLHYEKLKTDARIEIAREINKDFFSTGLRQQGTRHGHQRPPVAGGARMGDWPMRNEYYGIIPHYGRGREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGH9</accession>
    <name>A0A1E3NGH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_12952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016332.1">
        <property type="nucleotide sequence ID" value="XM_019159991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGH9"/>
    <dbReference type="GeneID" id="30176678"/>
    <dbReference type="OrthoDB" id="3992193at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="504" mass="57011" checksum="20BC51E0DFCB4E02" modified="2017-01-18" version="1">MATEVIQQPPIQYFNKDTRSSSSNYSVYTSDAHHRIASGDFIFQSSANSKNPGKRKSLTKLTENELKEQPLSNLKSPGHGRFSKANLKKEFTVVRKNSYKKPSYFRLSHSASSPKSLKKAAKFQNSRQIEPAFDMYPSKSLLENPSYMVQYFTTTLNLIKKDPAIHYQILKAHIDTKNNTDNEAELTRRTRKNKYNSLPIMGSSKLLKEEMDQARNIKRNSAPAVLSYQPTDMTMVEYLNTSLDFYKHRKKDSEATTTGDSKETKLSSKAQTTSSRTQPGSENKGGSSGSGADSVSTSALYARRSKLTYHPEGTTSSQRSYPLKTAKRNSILKRRSALPYDTGSENASFHLQRELSASKKEEYFSKYGTELLENAAGKTIIEEENPNRKRISDCGDLSIATGNELYNQVKNEVASKRLLNWDSAYRYIAKKDPDLAENYFFLIRYMLELYLRRVLSAKIALKLGTDKSNKGEGNEAWIGLRECIASVYGEDNALFFESHNARKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGI9</accession>
    <name>A0A1E3NGI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016342.1">
        <property type="nucleotide sequence ID" value="XM_019161710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGI9"/>
    <dbReference type="GeneID" id="30178397"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="58" mass="6926" checksum="D58252DE61B5FF52" modified="2017-01-18" version="1">MHTYQNESLKSPYLIKPSVTLRYSISAIIVLRYNWMFLSLQLLERLEMTLRCRSTCLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGQ9</accession>
    <name>A0A1E3NGQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein ORM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_17802</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016453.1">
        <property type="nucleotide sequence ID" value="XM_019161751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGQ9"/>
    <dbReference type="STRING" id="763406.A0A1E3NGQ9"/>
    <dbReference type="GeneID" id="30178438"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018147">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ45340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24373" checksum="3AABE97791301943" modified="2017-01-18" version="1">MSPSSPVIKAEPFPAPPSSPEKLALSPTTSHAQDKGHRRRRSSSLISKVEPETFETKQDQDVMDNMNANWVHFKGAWIIHIVVIMMLKFFYDLMPIFSNEVSWTLTNVTYVIGSYIMFHQVKGTPFDFNNGAFDNLNMWEQIDNGAQFTPVKKFLLGVPICLFLISTHYSNYDLTLFVINLIACLIVVIPKLPSSHRLRINIPYITDQQIVQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGZ5</accession>
    <name>A0A1E3NGZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015951.1">
        <property type="nucleotide sequence ID" value="XM_019164796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGZ5"/>
    <dbReference type="GeneID" id="30181483"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="122" mass="12821" checksum="F5C724F1E3B490C7" modified="2017-01-18" version="1">MNPAAHEAQKLPDISTNTFDYQESDRAIALVDPPSENPASVPSDEDHIVASSEPYFPSASLPDCVPDAVPSVVPVLNLLPRVSSQLSLSSVASDTELLGLLQDDSPPDASANQAITVFFACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH44</accession>
    <name>A0A1E3NH44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_60190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016564.1">
        <property type="nucleotide sequence ID" value="XM_019163414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH44"/>
    <dbReference type="STRING" id="763406.A0A1E3NH44"/>
    <dbReference type="GeneID" id="30180101"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000066">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="48"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ45451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="443" mass="48670" checksum="EC95A17FB7115EF3" modified="2017-01-18" version="1">MLGRHTITARRLGSRGLSFFSGIELAPPDPILSLTTAYHADPKPEEYKINLGVGAYRDGQGRPWVLDSVRQAEQQIAADKRNNHEYVPILGTSTYVGLVQRFLFQQEGMPFSTRLLVDEDRIATGQGISGTGSLKVMAELLAKKVKSDSGRAGGRVFIPNPSWANHASIFASSGMEVGTYRYYDPQTQGLALDALLDDLAALLPGDAVLLHVCCHNPTGVDPSDAEWDQILQVVEDRQLLPIFDMAYQGFSHGIAEDLAVVEKLARRMLSSGLIENAMLSQSFAKNLGLYGERVGSFSLITASREEKAKVQSQLKQIVRSMYSSPPCHGSKIVERVLGDEQLYAQWCGEVAVMRDRLKQMRLLLFEQLRNLRCHGPGTTGPTGWEHLVQQQGMFCFTGLSPDQVNALQKQSIYMTSNGRISVAGINEANVERLSRAIAGVFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHE2</accession>
    <name>A0A1E3NHE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">V-ATPase proteolipid subunit C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_16850</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016660.1">
        <property type="nucleotide sequence ID" value="XM_019161363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHE2"/>
    <dbReference type="STRING" id="763406.A0A1E3NHE2"/>
    <dbReference type="GeneID" id="30178050"/>
    <dbReference type="OrthoDB" id="10264021at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18177">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18178">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000002">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="128"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ45547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="195" mass="20223" checksum="0D648603DE4EF588" modified="2017-01-18" version="1">MSSVYTSIVTFALLTVGGYMLFNESGEAFNVGAFLESVSPYMWANLGIVSCIGFSVVGAAWGIFITGSSVIGAGVKAPRITTKNLISIIFCEVVAIYGLIMAIVFSSKLVAVDNILTVENLYTGYSLFWAGLTVGLSNLICGVAVGITGSTAAISDAADSSLFVKILVIEIFGSILGLFGLIVGLLMAARAGEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHP4</accession>
    <name>A0A1E3NHP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_73131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016729.1">
        <property type="nucleotide sequence ID" value="XM_019164387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHP4"/>
    <dbReference type="STRING" id="763406.A0A1E3NHP4"/>
    <dbReference type="GeneID" id="30181074"/>
    <dbReference type="OrthoDB" id="5374328at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018287">
        <property type="entry name" value="Hap4_TF_heteromerisation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF7">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10297">
        <property type="entry name" value="Hap4_Hap_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="24"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="285"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="480" mass="52279" checksum="981FAC36C8777CB6" modified="2017-01-18" version="1">MPTSNRTSTNFSVKGDEGKPITKIVMSKEWVLPPRPKPGRKPCDDIPSSKRKAQNRAAQRAFRERRANRVSELEEKIMEMERDRSVSEGLLNNMIKTLQNENSQLKSENAGLRGDNAKLVQRLKDSAPAGGATSSPERMSSLESLFNLEPQSAVPLKRKSSSSVSPSTRSLSKGEIDFTNAFAFKKQRKMPVLNDIFFSKSESVKPESKEQTPSGSGIPLSARPGSSSSATPTPLAAPTTTAATTATTATTAATTNASTTTSTISNNIPFESCGFCSEDTPCVCREIETEQQQQMQREIRNIKESKLKIEIEKKERELIMELTRSAKKDDMAQIAKCTGNPGTCLQCRNDPLSNLFCKTIAEKSSEEEIVLPSISRNGSISSMNDAAVSISNSTSSSISNPGSTSSSKYRLPSLESLKLNEAGKQFVPCADAYKTISHHLDIRNVGVNNIANNLQTKGMFVEVGSVVSCLRELDRKFSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI52</accession>
    <name>A0A1E3NI52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RecA family profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_64176</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016925.1">
        <property type="nucleotide sequence ID" value="XM_019163477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI52"/>
    <dbReference type="STRING" id="763406.A0A1E3NI52"/>
    <dbReference type="GeneID" id="30180164"/>
    <dbReference type="OrthoDB" id="1861185at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF66">
        <property type="entry name" value="RE19845P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="4">
        <location>
            <begin position="92"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="464" mass="52414" checksum="87B147EF70A7A8B7" modified="2017-01-18" version="1">MDLTKYCADSSFILNKPYCESLLNTFRSNQITTPELLLSSISTVARKLNRTESEISAFIDKFEAETCDRVVKSLHKPIPFLTQKNDQPPAAEPNLFSVGDESIDNILNGGIPTGYLIELSGKSASGKTNFLLTLSVTIQLPLEFGGLGPSIFSTESTSHVGVKTMYIPTESPLATQRLKQIIDSFTELLETNGISFENQMFFPKLDNVLTTSNVMTNLEEQDHILRYQLPVMLERDPSIKLLIIDSLTHHMRAQLTWSEQRSYVQSLCTYLKGLAKHYNISIMVANQVTDKPVRGLYSGDNDVLWKLNTEYQLGWMNGWDDIGIMYRQLMRREGVIDEAGKSFERLDYLNDIQTCGHSNLSQREGDSYSSDSHYDSKSQSTMKNDLQNILKSEQKRLFDSNYKVKVSGIGTRPALGLALLDYIDMRIVLSKEYVPIFDEKLIDEFSVELGIDTSNLEDSFLCST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI81</accession>
    <name>A0A1E3NI81_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_73336</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016955.1">
        <property type="nucleotide sequence ID" value="XM_019164452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI81"/>
    <dbReference type="STRING" id="763406.A0A1E3NI81"/>
    <dbReference type="GeneID" id="30181139"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000007">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2420:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000015">
        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000183">
        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007638">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007639">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042558">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042559">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04558">
        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04557">
        <property type="entry name" value="tRNA_synt_1c_R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="15">
        <location>
            <begin position="13"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="14">
        <location>
            <begin position="179"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="262"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="13">
        <location>
            <begin position="579"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="16">
        <location>
            <begin position="692"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ45842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="815" mass="93305" checksum="AEFF1953B61DF6C4" modified="2017-01-18" version="1">MSKTSATEATSEEELSKLFSQVGFEDKKIKEIVKNKKVSQSLADVIESSDILENSAALPLDKSENALLHYLASLLKGKNVDGIDHISRSIIDKKLKSNLQVAEALKYVETHPDNFVESDFEIASGVGIEITETEVFKIICDYISEHKEEIETNRYRMVPALLANVRQLPALKWASPALFKPTIDTQLLELLGPKDERDVIKKEKKKKETKETTKDDKPSSLTNVHARNMFTEGFLGDLHKPGEEPQRWDDVIKAHRDFIKGKVYTRFPPEPNGFLHIGHSKAIMVNFGFAKFHDGKCYLRYDDTNPEAEEQVYFDSILRCVKWLGFEPWKITYSSDYFDQLYSLAEKLINSGYAYVDHSTAEEVKAQRGVKPDGTPGGERFPSPWRSRSVEENLIEFRKMRDGHYKPGEATLRMKQDIDSPSPQMWDLVAYRVLNASHARTGDKWKIYPTYDFTHCLVDSFENITHSLCTTEFYLSRESYEWLCDVLHVYRPAQREYGRLNITGTIMSKRKIAKLVKEGYVRGWNDPRLYTLESLRRRGIPPGAILSFINTLGVTTSTTNIQATRFDSAVRQYLDHTTPRLMMIENPLKVVLDNLPDDYEELIEIPYKPGNDEFGSRKIPLTKVVYIDSSDYRDETSKGYFRLAPDQPVGLMRVPFNIKVKSVEHDSNGKPVLIHAVYMNDLSEHKKPKAYIQWVPESAKYKSPVNVSEVRIYNQLFNSENPSSHPDGFLADINKESEEVIKGAMIEPALLDVKAKSPLNMPFTDEKFSIKEKAGFETVRFQALRVGYFCLDSDSTEDKLILNRIVTLKEDSDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI84</accession>
    <name>A0A1E3NI84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar ATPase assembly integral membrane protein VMA21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_59448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016944.1">
        <property type="nucleotide sequence ID" value="XM_019163368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI84"/>
    <dbReference type="STRING" id="763406.A0A1E3NI84"/>
    <dbReference type="GeneID" id="30180055"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="5997" checksum="0FFE126D02355AA1" modified="2017-01-18" version="1">MILAPLVAFFGCNSIFSNSLVSGGVAAVVANVVLIGYVYVAFNEEIDTEGEKSRKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIB6</accession>
    <name>A0A1E3NIB6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="9">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_17140</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="9"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016990.1">
        <property type="nucleotide sequence ID" value="XM_019161479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIB6"/>
    <dbReference type="STRING" id="763406.A0A1E3NIB6"/>
    <dbReference type="GeneID" id="30178166"/>
    <dbReference type="OrthoDB" id="10263760at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000294">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017751">
        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="10">
        <location>
            <begin position="36"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="11">
        <location>
            <begin position="234"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="41"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="310"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="388" mass="42839" checksum="BEE1577999B24CA4" modified="2017-01-18" version="1">MVSPAERLSSIASTMIPHRKDSTTLAPEDYPETPFKVTVVGSGNWGSTIAKVVAENTVERPLQFQRNVNMWVYEELIEGEKLTEIINTKHENVKYLPGIKLPANVVAVPDLVEACADADLIIFNIPHQFLPRILSQLKGKVNPKSRAISCLKGLEVTPDGCKLLSTYITEELGIYCGALSGANLAPEVAQCKWSETTVAYTIPHDFRGKGKDIDHQILRALFHRPYFHVRVISDVAGVSIAGALKNIVAMAAGFVEGLGWGDNAKAAVMRIGLTETIQFAQMFFQNCQPSTFTNESAGVADLITTCSGGRNVRVGKYMAQHQVSAQEAEAKLLNGQSCQGMHTCKEVFDFLSNMGKIDDYPLFDVTYRIIYENFPMEKLPEHLERVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NID8</accession>
    <name>A0A1E3NID8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017010.1">
        <property type="nucleotide sequence ID" value="XM_019161485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NID8"/>
    <dbReference type="GeneID" id="30178172"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="218" mass="24686" checksum="69B4C0D2E6370C1C" modified="2017-01-18" version="1">MTPRHRAYIARMQGHPEQAMFRSEDYRECTPGAARKTSEALKSEVFYVDQNLHNAVVFADISQEYRESNGGCMVQEMENSSDLTLVNSTFISSEPALEESDFQRATVEESCGLHPGLKRMLEPVEEEQPPGKRCRVGNERQLRQLHHSRYISSDFISSEGIEISTIDTHSYSPFEVSQPFSQFSQAKSSQYTPFPLYTAQDISHAVLSSFDPIFGSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIJ7</accession>
    <name>A0A1E3NIJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017009.1">
        <property type="nucleotide sequence ID" value="XM_019161484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIJ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NIJ7"/>
    <dbReference type="GeneID" id="30178171"/>
    <dbReference type="OrthoDB" id="6585699at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007902">
        <property type="entry name" value="Chl4/mis15/CENP-N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05238">
        <property type="entry name" value="CENP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="570" mass="64497" checksum="AB69E64A4E330D27" modified="2017-01-18" version="1">MLPRKRKLSNSGILSNSYIPPLSAAQIERELQKLPPDSLYSLTHVWLSIPATKPLPDANQKKQGYTKDSLSRELLKKLELLKNIKVKINMKKKLVNTILVEFYPRGLNTLQLAQIDVQLLVDKPSVNSWVSSTAKIVSSTSDIPEEGDKMREKKLNQLSNYTISLDSQLFLDNFVLNLSNLFLTHVYISRHPHYPLILIRVQMYNYSYRRPDDSTSSTIISDLTNSSLDRTAKLKFEQDFQRIVSNKTDSKRGKAATNGVQRQIQIQNKPQITPHKPFYILLPISSPHVIHTPPVGDSVGTKLILQTLETTLSYTNVQREAQKGNIGLNSSILTKTKIKIFPDSDVPTPIRNLDAIFTLKGISRFGSSMGPWIPYADDLVDMGVLGNELRHQILQPEQFVDLSPGAESDPEHAEREKVAALKFKGCMRKTKSKELSNAKNKENYEHDTQGEYDIPNNDQVDDGAREAVNDIYASIVPIHECNFEIEDTLSQFFSQRKRKGTTQRKPKRDPAAREPPFRFKMSFFGSDVFGGLHELAVNEYVNPYETPDWLTGESGTLGGVVKNGRFRKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ13</accession>
    <name>A0A1E3NJ13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_35366</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017247.1">
        <property type="nucleotide sequence ID" value="XM_019162938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ13"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ13"/>
    <dbReference type="GeneID" id="30179625"/>
    <dbReference type="OrthoDB" id="2161780at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF8">
        <property type="entry name" value="CYSTEINE SULFINIC ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="56067" checksum="D18720312FCA1105" modified="2017-01-18" version="1">MTRPADCDLEKNRVQELQDILKPAIDIIINYVKEIDNGERDLGPRFEDPKELYKILELEDLEAKFQNAVQTETSVSSTEMLKVFGKILDNSVTTWNPGFMDKLYVGSNPIGILSDLLLSILNTNSHVFTVSPALSAIERKVSQRYANLYGFSDKFAGGLTFSGGSWSNITSLQIARSIKYPETKLEGTSGHKFAIFTSAHCHYSVEKAAILLGIGANSVFKIPVDEVGRMNVKILSQSIQERIAEGYTPLYVNATAGTTVFGSFDPFEEISAVAKDYNMWVHIDGSWGGNVIFSPEKAHMLKGSHLVDSITASPHKMLGVPTTCSFLLVPDERIFTQANSLHAPYLFHNARDDEENFDLGNGTMGCGRRSDAVKLYLAWLYYGQGGLKKRVEHSYAIANYFVAKLKETEGFTLINDFPIPCLQVCFYYNPVGEKLTGAEMTVRTRRIAKALHCSGEFLIDFSPNPNDALTGHDNGEFFRCVFNSPNITSSIVDRLVDRIVALGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJ56</accession>
    <name>A0A1E3NJ56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73612</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017254.1">
        <property type="nucleotide sequence ID" value="XM_019164536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJ56"/>
    <dbReference type="STRING" id="763406.A0A1E3NJ56"/>
    <dbReference type="GeneID" id="30181223"/>
    <dbReference type="OrthoDB" id="3981028at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="450" mass="50211" checksum="567A4E9C2F5FC78F" modified="2017-01-18" version="1">MFAQHGSFDSRRTNGSPSPLVTIDLTTKFTTQYNLAVKYYLHRNFNKSFKLVQPVVEELRTNPNVVDPALVAKLAKIYFSLLSLLLNEVINTKLLRLSIPRHSLNFPDYLSEKEDTHFADAILDDFNHDGLYRLVGDLHLSDNEEIVLMCFMTESSNNMKTESLQRQVESYLSLRGLYPGPISDSDLGADNGEGSTLRKIVQLYLSQIILKYHGVEKAVNVIERMFVTDETGVQKWVAWILACDKTARSANSEEEDDDENDDAIYDESYGESDDDGYASDNTAITTNTTATITSKPSTSLAEDRVHGLAARTLSTQSALTHTTLTETPHAASSNGLELLESKKKGRSKTKSKGRGKSKSKTRSGKSDDSSLISMTNSYMTRQLTTFKDLVAHHVSRYPLLVRLATFVIFLLLILGRGSGGLNQVQLKKRLVAFYSKMVQTAQLAFKCSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJE7</accession>
    <name>A0A1E3NJE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_27463</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016811.1">
        <property type="nucleotide sequence ID" value="XM_019162497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJE7"/>
    <dbReference type="GeneID" id="30179184"/>
    <dbReference type="OrthoDB" id="532630at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ45698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="103" mass="12501" checksum="A176F7151DC6E70F" modified="2017-01-18" version="1" fragment="single">MHPILDRDRFQNCEDLIDALEECHRSPFFETVLGKCSDVKIQLSQCLHENRLANDRLQILQRKEKNKLLEEKKKKREEEEWGENGYLKKVVELEYQKRMQQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJI8</accession>
    <name>A0A1E3NJI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial carnitine carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_17020</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016851.1">
        <property type="nucleotide sequence ID" value="XM_019161430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJI8"/>
    <dbReference type="STRING" id="763406.A0A1E3NJI8"/>
    <dbReference type="GeneID" id="30178117"/>
    <dbReference type="OrthoDB" id="14252at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005476">
        <property type="term" value="F:carnitine:O-acyl-L-carnitine antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF4">
        <property type="entry name" value="CONGESTED-LIKE TRACHEA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="104"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="195"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="29402" checksum="1340C5F6699ED789" modified="2017-01-18" version="1">MEEIDSVLVDNVKSFAAGGFGGVCAVLTGHPFDLTKVLLQTNEYPSAAKAVRGIVAHDGPLGLYKGVTSPLLGVTPMFAVSFWGYDLGKRLVMGSRGKSPDVPFTIAEISAAGFFSAIPTTLIAAPFERIKVVMQIDKTQATFLQTVRTLYKQGGLRSIYKGSAATLARDGPGSAAYFATYEYVKKHLSGGSDELSLGAITVAGGCAGVAMWTAIFPVDTIKSIQQSSTVKTTMSEVARTIYRSKGVAGFFPGIGPALARSFPANAATFLGVELAKHVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJQ4</accession>
    <name>A0A1E3NJQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72436</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017472.1">
        <property type="nucleotide sequence ID" value="XM_019164195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJQ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NJQ4"/>
    <dbReference type="GeneID" id="30180882"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071232">
        <property type="term" value="P:cellular response to histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071264">
        <property type="term" value="P:positive regulation of translational initiation in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000882">
        <property type="entry name" value="Translation initiation regulator (Gcn20)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="196"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="529"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="287"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="749" mass="84512" checksum="10E44F09EAFF709A" modified="2017-01-18" version="1">MSNLGLQVRQAAGSIDSVVADYAVGYMQHILHSTEDAVLARQLDITKEVSFIEELLVSAGGDSNKVRQLGEKLQQNIEKQLKDNMSKLEITGDTSKRLLDISLINSKQRELDSSLALLQSTDIEHTGRSIESRVDKKKLQKAEAKIAKKVAKRNNRFVQYEASKLINDQQKDDYDSFFLKVNPIEFGAGAGKSKDIKIDTFDLYVGEGQRILSNASLTLAFGRRYGLVGQNGIGKSTLLRALSRRELDIPKHITILHVEQELRGDDTIALQSVLDADVWRKQLLTEQERIGERVDEIEKLKEEFDEESLEMKKLENEQVDLTDKLEEVHEKLGEIESDKAEARASSILYGLGFSQEAQQRATKSFSGGWRMRLSLARALFCKPDLLLLDEPTNMLDVPSSAYLGDYLQTYPATVLVVSHDRAFLNEVATDIIHQHSERLDYYRGANFDSFYATREERIKNQKREYDNQKAYREHLQTFIDKFRYNAAKSSEAQSRIKKLEKLPVLEAPEEDKQITFRFPDPEKLSPPILQLLDVSFGYDPKNMLLNHVDLDIQMNSRVALVGANGCGKTTLLKVLMGTNSPLDGSVSKNSRLRIGYFAQHHVDGMDLTKNAVEWMSEKYPGKTDEEYRRHLGSFGITGPLALQRMQLLSGGQKSRVAFAALCLTNPHVLIMDEPSNHLDTSGLDALSGALQRFQGGVLMVSHDVTMIKQVCKEIWVSEDGRVYRFDGTIDDYKKYILSKANASGVVKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJZ4</accession>
    <name>A0A1E3NJZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_72526</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017570.1">
        <property type="nucleotide sequence ID" value="XM_019164220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJZ4"/>
    <dbReference type="GeneID" id="30180907"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057407">
        <property type="entry name" value="HEAT_TANGO6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23565">
        <property type="entry name" value="ARM_TANGO6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TANGO6 HEAT repeat" evidence="3">
        <location>
            <begin position="253"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="2">
        <location>
            <begin position="680"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="933" mass="106203" checksum="53D2A79A5EA3F7F8" modified="2017-01-18" version="1">MPELDQERLQSLRQELAKFKRGERVEKRSRTTALGKLFDAITKKIEGYQTESDVISFLYAQFSPHQEQAGSAARRKYVIDELLGYLREINVLSAKKKDSNMISIPLYDIRVVGELTNIIIIHGVYAIMPSDILLPLEKRKLKNFKAPTAYTKMDFNSGIPILKDILSTFTEIFESESDLKDLILVGTGFTDALSIAIYFAVSIGLAEYQQYIDRLEAQSSTYQLLSFYSILLKNCRANRTVATFVSFLLSKQLVKRNGVESLIDLALGLREDEEVDIAKINSIVQVLLTSKPQSMSLIDYYKNIFSQIYGMLVLVNRPLMNTILVEMITVIYKRNKRIIADFLFSDVWENLNPALRKDRDISTDESVVLTNEIDLNNSFNVCLSISRSLNNLDQEIMNEFFQPILLSLWYYANYQRSKQKDYDIVLNLVKNVIILGDSDHFIDLITTNLIDYQMEWKFSDGESNLTFIKYNTKESETSKENKILELFNKIDFYMETFVNLTSKLNDSDIDHTNKVLIATLNKLYSNTALDDTDIPFKKIVYLKLIQSLLETFKSEIEGSPKSLLIFAKTYFNQYFESVKSKSKLDIVKTEDSDDEDEDEVLFDGSEGGDGITSLVPILDIISTLSPSSDSEKEQMESLQITLKTNIKYIPKSIYQVCEDIIGIDTKNTTIKSSSSGFNMETVLKQINDPTPSVRVYALDKLTNYTINSKTEERKQVSTKYTLNLLLSQLKDLEPFVYLNAIKNVVSVLSFDRTFFSYVIELYSTSKKSIDEKLRIGEILTKFISSHGKVLVGSQIKVIINTCIKISRAESKTEKKLADNSDLRMKMSSLSILGTTCHECGYSVIPYITEIADLVHGIITFETSAELKRAAVVIINDIVSSDNGLEIIKDHGEKLQILLDYISENDHDLLVCQLASATLDQIEETFNSRFTILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK07</accession>
    <name>A0A1E3NK07_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_16364</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017598.1">
        <property type="nucleotide sequence ID" value="XM_019161180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK07"/>
    <dbReference type="STRING" id="763406.A0A1E3NK07"/>
    <dbReference type="GeneID" id="30177867"/>
    <dbReference type="OrthoDB" id="2357318at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="669" mass="76468" checksum="87FCCD0777F7FBA0" modified="2017-01-18" version="1">MSGLGYKLGQQGRQILNSNNVTAQDSYGAIPKVQQFQLPTELDYLLYDIANPKPSTSADKVFSYLVHYYPRLKSSVNIQLLTKHFLRCPLFFSSLKDQTLENITKIIECYQYIITTKYKVSNPTISFHEFYISIYDSIRDVLLHDSTAHWKILPVLAGCISSISSMELYNPCPSHHNVIAKVNKLELSLFSDSFLYVIRSPLLPEFKDNILVSLIYTQEHLSSSFYAGLTNADPEILPELMRILFFSKYGLDKGSLLSSNISYNDIMRTKPILRQLNKWAFLFGKIAKASKDPLQNLKAISRSLDYIVSFAINISNAGLETLFEASDKWDLVKYIFFTVVMIFEHATREIVLGTSRINQFAFMISNQILRSFFYLSYVLDQIGTGGFDSYNFIFDSTTSLLGEYNPDLTESLLYSMLNEIPVSKRQTPANESKLNYFFRTTEATLQSLKKSFKETTLFPLMNSVFDSNDSSAPTVEFCHSIMVKHLNLLCMNLSESENEAHLQEAVFSCFNRVLAQFPKNLSLNQTSLITQTCGKAAAISSPNDPEFLNTLLDLVRFQINMSAYTPLPPRISFVNGDRMTVPEKLCTRRAGLISIYIDLLQFTSINSFTNILEDIKHQIDNLVGSQDVYFLYDVLWDNILLVNKYDCQKGQIGIDWWYRRVNKELVPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK83</accession>
    <name>A0A1E3NK83_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_72560</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017609.1">
        <property type="nucleotide sequence ID" value="XM_019164235.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NK83"/>
    <dbReference type="GeneID" id="30180922"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16567">
        <property type="entry name" value="RING-HC_RAD16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000753">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008626">
        <property type="entry name" value="Mediator_Med15_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05397">
        <property type="entry name" value="Med15_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="1596"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="1951"/>
            <end position="1995"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="2034"/>
            <end position="2188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="85"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="225"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="418"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="575"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="933"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1339"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1479"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="275"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="226"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="428"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="575"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="635"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="960"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1369"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1383"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1404"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1486"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1517"/>
            <end position="1533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ46496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2205" mass="247111" checksum="4887E8BAE5F16E2C" modified="2017-01-18" version="1">MNGSNQGFLPNNQQQHFRLQISPNDRLQFIKVLITSLQKIQGPSFSAQKVRPVAEEFEKYAFSHSSDKSQYVDFLKSKIASLTQARRANQQQQQQQVPQQMPQQMPQQMPPHMSPQMQQARTQQQQNLNQHHQPAPPQQQPQQPQQPQQQQLQQPQQGGKINLHTAPIPKELLDKLPKVFPPMLNTWQTIMPYLRNNQLSQEELQTIRNVHMMHVNLFKQNYANMQPQQQQPQQQQQQQPKPQQQQQQQPQQGQPQPNSSNASLNPQQILMMNRQREVQAQAAQAQAQAQAAQAQAQAQAAQAQAQAQAAQAQAQAQAQAQAQAAQAQAQAAQAQAQAQAQVQAAQAQAQAAQAAQAQSHSNSQQLSQAKQAQINAYKIQALQVIRKLQSLGKLPATLTPEQEAAYVRKYLLHILQNTNRDSQDPSKQQQQQQQQMMRQQQQQPQSTQQQMQQPPQQQPHYPQQPQQQQHQPQHQQQQLKPNVQANQNPMISPVLQQAHTQNMFQQSANYDNMQLPLQVPKHMANSNSIDMSTLSSPLIGMQNLNTNTNQGVSNFGSPTIQNPQLMVKNQLPIQMQQPQAAAPKQQLQQQLPQNFQQQQQQQQQPQYSLQQAAQQSSARKQQQQQQRQVYPSQQLPTHPVQQLPTNQNQFGNAISNQMPANQFINTNSSGAKLLPGLKATNEDWTALKEIYHETLISPINLKDMTTTLSDDDKRKVLGLAKQSKQLMVLCQSIIIPNFYFLTKQRDATKRIMYSELMLKQILEKLSLSGKYVADENLIHRIHDQIKKYLQFVKDQSSKIVKAQVQAQQVRAQQLQQQMELAGNVPPKGTFNQAPVPNATLTPSTIQNFQNRPVQDSFNADSSQFNLQMMNNVKGNNNMNATGNLMVPTPSAIQPGGDNMKPVMNNLQANGAQLYPSMNAPPMQNVPVKPAVISSSASTPSQIKKPKRKPSKSVGKISNPNTPASSNTVASTPTSTTVAPNRNAAGNTVGATAAPTAASPSNVNSAEIQAVLKVNNENVEKEEARFKAIDVARARRRMLVSSGTQPSNAASSPVASANLQPISGAAAYFLSTLCDTLGLESEEKAIISNANNSSLDGTNGGIPGFIRTKNNGNNGGKVLTPSAVLATPIGLNAKTPVAKYPSALSPEELKILPWTGKISADCIASCFRALDSKNLNPSPSFLSAAPATPVGHKCSQTMSAGSTKSFVQLVQERQSKRKTMSILCGGEDKKWGVDPDSPWNWNTKKQTDGLKVYPTPPEDADCAKKRKLVSVYPVKNEDDDSAAIKKFKTEDADYNLLWDFDKAELVYTHEDFISLLSDNDEVEVGIEAGFIITEMVPPRKRTKSGTRVNQPNVPVRSRRTRSTVKKEQDTDVADVEENGVDNNGDVKAEEISHSDKDEDFHISDIDEDIEDGDDDDEDQDYVDDIVITGHRSRGQKFNPVELSDDDIVVFSGKPSKNNGRHGPGIEVLELQDEVTELLDDQNGNESIEVEQEGQPQEGEEEGEEEGEGGGFVKDEKKTKKRKAPAKRAKKKKEPKPKLTPFERRTNKLYESHPELRDVFPRLEERAAKKVERAEQPKNMAVTLLNFQLEGLSWMLRQEEANEYKGGILADEMGMGKTIQMLSLMMNDTTKGPNLVVAPTVALMQWKSEIETHTNNFLKVGLFHGASRAKSLSELDGFDVILTSYAVVESCYRKERYGFTKKAKKVKEKSILHQKKFYRVILDEAHNIKDRTSSTAKACDYLNCEKRWCLSGTPLQNRIGELYSLIRFLDIAPFCEYFCTKCPCTSKEWNFPDYRVCAQCGHAPMLHMNFFNHFMLKNIQKYGLQFEGEESFHRIQLLLRQIMIRRTKVERADDLGLPPKIVEIRRDFFNPEEKDLYQSLYSNSKRQFDDYVSEGVVLNNYANIFTLITRMRQLADHPDLVLKRMKVPGKKGGPGAKKGNGFDMEVLLGVIICQLCDDEAEEPIESKCHHKFCRICISEYVESFNGDTSKLKCPVCHIGLSIDLEAAAIEMDMDAVEKSSIVNRIDMGGSWKSSTKVEALMEELYKSRSDRVTTKSIVFSQFTSMLDLVEWRLKRAGFETVKLQGSMTPTQRQSTIKYFLETPSVEVFLVSLKAGGVALNLVEANQVFILDSWWNPSLDTGQAADRIHRIGQHRPIRIVKLVIEDSIESRIIELQEKKANMVKATLDKDERAANQLSAADMQFLFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKG5</accession>
    <name>A0A1E3NKG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NEDD8-activating enzyme E1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72667</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Regulatory subunit of the dimeric UBA3-ULA1 E1 enzyme.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017723.1">
        <property type="nucleotide sequence ID" value="XM_019164268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKG5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKG5"/>
    <dbReference type="GeneID" id="30180955"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030667">
        <property type="entry name" value="APP-BP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF29">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039099">
        <property type="entry name" value="APP-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="7">
        <location>
            <begin position="10"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="516" mass="57376" checksum="7148072D01B46AA8" modified="2017-01-18" version="1">MEDSNFGSKYDRQVRLWSGNGQRSLAGSRVCVVNGSPWASEILKNLVLPGVGSVVVVDSAAVGEEDLASNFFLGGGDVGAQRAVRLAETVSTLNPDVCITAVVDDRALFGDGDEDAGFWSSFDCVVLCAGPAYHACESRLSERLWALGVPLLKVGAVGFYAYLRIQTGEQVIVETHESNLHDLRLDLPWPALQQHVDRTSLADDSYLRAPYSVVLSKVFQQLQQNADGSRRPAPAAVRAALKRLYRTGDELNLDEAYSKAYLLMRNSAEIPPSVADILADRKTTHLDGSSSLFWILCNALKCFYDTFHVLPLSGVLPDMESDTADYIRLRNIYTDKFEQDRRFIRDKALLSLRQLHRSPDELADNDSLLTLFVKNSRFLKVVRGSKWDTMPKILEVFKSGNEQLADKALIYLAFLTLESFAREHHRSPTFDDRPELRTTAIAILCNNHDVKTFPEGFDKVLDEMCRACGRSLHNVCAVIGGITAQEVIKILTNQYIPLDNCLVYDGITGRTSTFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKJ7</accession>
    <name>A0A1E3NKJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_72696</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 32 different proteins and 1 molecule of RNA (18S). The 60S subunit contains 45 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017754.1">
        <property type="nucleotide sequence ID" value="XM_019164275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKJ7"/>
    <dbReference type="STRING" id="763406.A0A1E3NKJ7"/>
    <dbReference type="GeneID" id="30180962"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="8">
        <location>
            <begin position="120"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ46641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="264" mass="28644" checksum="9B4C077272189C57" modified="2017-01-18" version="1">MAPKGKKVAPAPLTSKTSKSASAKNPLFESTPKNFGLGQAIQPKRNLSRFVKWPEYVRFQRQKKILSLRLKVPPAIAQFSNTLDKNTAAEAFKLLNKYRPETSAEKKERLTKEAAAIADGKKKEDVSDKPYVVKYGLNHVVGLIENKKAKLVLIANDVDPIELVLFLPALCHKMGVPYAIVKGKARLGTLVHQKTATVAALVDVKSEDESALAKLVSTINANYIEKYDDSKRHWGGGIMGAKAQAKISQRAKAAAAAAAASSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKP3</accession>
    <name>A0A1E3NKP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_16528</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IWS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017804.1">
        <property type="nucleotide sequence ID" value="XM_019161242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKP3"/>
    <dbReference type="STRING" id="763406.A0A1E3NKP3"/>
    <dbReference type="GeneID" id="30177929"/>
    <dbReference type="OrthoDB" id="21124at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="245"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="349"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="447" mass="49377" checksum="0C6BED9FF4E6DDA1" modified="2017-01-18" version="1">MVSEDLVAAKLEESSHDGLLDQSVDEHQESGESAAADGDEEKEGEEKADEDAGDLKEEASMAEAEVEARMDASESEETAANEAEQEEPLDAGDGDIEEDISKLNKFKKTDGRSASPGQKRGRDSDFQDDASSSQAAAASNGAGIDGNEEIEDEQTRKRREFEEKFNALLKKPTKRRKNNDVDLEAMQDEAISHLKNMMKDAAYMDIDCVNNKKPAANKLKLLPQVRDTLLKSNLYDSILDNNLLEAVRIWLEPLPDASLPSFEIQKTLLNELVKLPIKTIHLRESGLGKVMIFYQKSKKVDPALKRIAEKLIGDWTRPVMGRSDNYRAREVPTANFDIEKFKAKTKLQTGGADTAQSGRKSLYQESADRRKRAAAPEARTSVYTIAPQTSINALHAQRASGLSTGIGQSLNRDERFKALNKKLTKLSQKKKAGKKDGVSIEGRGLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKS5</accession>
    <name>A0A1E3NKS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_16580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017865.1">
        <property type="nucleotide sequence ID" value="XM_019161265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKS5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKS5"/>
    <dbReference type="GeneID" id="30177952"/>
    <dbReference type="OrthoDB" id="2015405at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050268">
        <property type="entry name" value="NADH-dep_flavin_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30466">
        <property type="entry name" value="FLAVIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30466:SF1">
        <property type="entry name" value="FMN REDUCTASE (NADH) RUTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="209" mass="23910" checksum="2F84F16DEBED1808" modified="2017-01-18" version="1">MILTASFSGKPEIGKMHGTTLSSVSSLTVNPEPIVQFNIQVPSATSKSIQDNKYLALHILKPSNDSVRLARNFSLGSRYINEPQPETRNTNNSSARKSTTPFERIDKDQWSLFENVMHNKRPGSIRLDSDLHLPILTKDSERILICQKYKVFKVYNHEIWTCKVKEILVNIDDDVKTGGLLYFDRKFHHVGEALIEVKKDEKEIQKLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKT4</accession>
    <name>A0A1E3NKT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">UDP-N-acetylglucosamine transferase subunit ALG13</fullName>
            <ecNumber evidence="4 12">2.4.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Asparagine-linked glycosylation protein 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ALG13</name>
        <name evidence="14" type="ORF">PICMEDRAFT_72787</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UDP-N-acetyl-alpha-D-glucosamine = an N,N'-diacetylchitobiosyl-diphospho-di-trans,poly-cis-dolichol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23380"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19510"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57269"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.4.1.141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Heterodimer with ALG14 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.141" evidence="4 12"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017860.1">
        <property type="nucleotide sequence ID" value="XM_019164295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKT4"/>
    <dbReference type="STRING" id="763406.A0A1E3NKT4"/>
    <dbReference type="GeneID" id="30180982"/>
    <dbReference type="OrthoDB" id="20273at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039042">
        <property type="entry name" value="Alg13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12867">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12867:SF6">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLDIPHOSPHODOLICHOL N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="13">
        <location>
            <begin position="95"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="222" mass="23436" checksum="81A89AE4F0D8A2B5" modified="2017-01-18" version="1">MTEGTAKQALVTTGATVTFEALIRAVLEPRFLETLAQHGYERVVVQYGRSERSVTVIRAALGALGDRVSVASERDEPDTGHTVCGTYHSDVGGVSLKCIQFDVRLIERYTRRSELVISHAGTGSIMDTLRVRAGAAQPARRPPRLIVMVNTGLQDNHQTEIAQAFESLGVVTSVATGAELTDAVVRTERAAAAAAAPTVVLPEACGGAVEAVVADELLPDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKT5</accession>
    <name>A0A1E3NKT5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">SWI5-dependent HO expression protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SHE3</name>
        <name evidence="13" type="ORF">PICMEDRAFT_72770</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SHE3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017843.1">
        <property type="nucleotide sequence ID" value="XM_019164291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKT5"/>
    <dbReference type="STRING" id="763406.A0A1E3NKT5"/>
    <dbReference type="GeneID" id="30180978"/>
    <dbReference type="OrthoDB" id="6088208at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031398">
        <property type="entry name" value="She3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17078">
        <property type="entry name" value="SHE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="271"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="14"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="278"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="394"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="425"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="483" mass="54525" checksum="60E626E8C31F9D64" modified="2017-01-18" version="1">MASHQPTSGTSKVIENLQTTIDSLKSELNETKATLSETKQKYAILSKRNESMVEQLSNVKHQAEVAESLLKRKERRVLDLESQITEVASSSDSYRFQCETLKNKMQNMESDKSRLQAENERLSKSYDILQSSFNDYKLSISHSVSSLKCQIPTFIDSTKNRLAENLSQLHSSQPQIDSSYNMMLKNSKRLEELYSQKYDKVNNYLILLAESTKQHGQSTSIIMEECEDILKKLNRNEDVLLKIKNETTGNLTDLDKMREFNKRRDFSILNDSSNIHNSQSAASSTISSPSLNSPSTSASPSFAAGTSTKAKNSLSSSLSNGAAKFSEKRRISPGILDSKFNPLNNTSRGSSPEVNENDSPRERSNNNNNTVNRDRKRHSLSNIPKKDQRRRSSYRQSMNLDDHANFNSDQQDDQSHIDRRKRVISSNSPGPYSPSSSNSSTLGRSNSTRRNNNRPNNRVSSTSSNPGNNNNHRRSWQPHDIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL52</accession>
    <name>A0A1E3NL52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_33461</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017977.1">
        <property type="nucleotide sequence ID" value="XM_019162826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL52"/>
    <dbReference type="STRING" id="763406.A0A1E3NL52"/>
    <dbReference type="GeneID" id="30179513"/>
    <dbReference type="OrthoDB" id="295033at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032302">
        <property type="term" value="C:MutSbeta complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000406">
        <property type="term" value="F:double-strand/single-strand DNA junction binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032137">
        <property type="term" value="F:guanine/thymine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000404">
        <property type="term" value="F:heteroduplex DNA loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032138">
        <property type="term" value="F:single base insertion or deletion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007534">
        <property type="term" value="P:gene conversion at mating-type locus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043570">
        <property type="term" value="P:maintenance of DNA repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006311">
        <property type="term" value="P:meiotic gene conversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000735">
        <property type="term" value="P:removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF35">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="11">
        <location>
            <begin position="793"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="995" mass="109912" checksum="2E84FA7463189040" modified="2017-01-18" version="1">MSANVYTEETESQPSIYRSYLRLAPTQQRTVRALVRSGSKSSSYYTLFDEDAILAAEIVYSSHSVLKTSPLIAHPKDLDKKVCVQYLPLSEQIFSSLLQTLILNKNYKVEIHTVEDGKNSTWKLLRSASPGHLDEIEDLIFASSSIIGALKLISSAKEGKKVGLAFYDPNLKFLGLSEYPDTDLFSNTESILIQLGIKECIIQSSPNQTASKDPDFARLTQTIDRCDILITQLPSSSFNSNNSDQDLVTLTNNEHILATSELASMTTALASSNALILYLGLLGNDSIRGSISVGKYDLDKYMRLDAAAVRALNLFPSGLENTSTVVNTTGISSAPTSIFQLLNHCKTSGGSRLLSQWIKQPLVDVKEIDDRHLLVEWFVQNPGLLGNIRSNFLTHVPDVSRLLRKLSGKRYSGLDEVVRLYQLVKKLDEGCTILLEGLEELQTADEDKKKRVSELVNFFWLDGLYEHMKVLGKFADMVEQTVDLDSLANATSAASASSLIQINPQYDKRLMELQEEREKIEQEMHVLHENAAIDLGMELDKKLKLEQNVNHGWCFRLTRNDAGVLRGKSSSSKSKNPSSSQYQELQTVKAGVFFTTVELSSLSSEFTRIMNAYASQQSAIVSEIRSITATYIPIFTKLSTVLDKLDVISSFAVVATCAPVEYIKPAKVWGLEEQGRQMRISQGRHPCLEVTEGISFIPNDYVLGPAQEKGKDFYLITGPNMGGKSTYIRTLGVVCLMNQIGSFVPCSESSELAIVDAIHARVGASDSQLKGVSTFMAEMLEMSSIIASAHKGSLVIVDELGRGTSTYDGFGLAWAISEHLAKSIKSWTLFATHFHELTALANEYENVGNLHVIAHVDEAAPEKDARETEDNVTLLYKVEPGISDQSFGIHVAELVKFPQKIINMAKRKAYELDEDAGDQAADAAKRPRYSKEEYAQGKELLKKILKEWKKQVLDAGGLESVDSAAAVRILHDLTTTGEYAAAVAASDVLRDTMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL53</accession>
    <name>A0A1E3NL53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_12501</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017176.1">
        <property type="nucleotide sequence ID" value="XM_019159936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL53"/>
    <dbReference type="GeneID" id="30176623"/>
    <dbReference type="OrthoDB" id="6428174at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF85">
        <property type="entry name" value="DUF803 DOMAIN MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G15880)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="38289" checksum="B524D63D74D568F9" modified="2017-01-18" version="1">MEDKYVGLFLAISSSFAIGSSFVLTKIGLLKDGSRGESLTYLRNPIWIVGTTMMAIGEIANFAAYTFAPPILVTPLGALSVIIGAVLASVFLREKLGVLGKLGCGICLLGSIIIVLHAPPDKEITTVDEILAYAERPLFLAYLFVVSTFSIFMIVKIAPKFGSKHPMIYISICSAVGSISVCAIKAFGIALKLTLSGNNQFSHPSTYIFIITVVVCILTQMNYFNKALAQFDTSIVNPLYYVTFTTATLCASFILFGGFNTASIVDVISLLSGFLIIFSGVYLLDISRHNPDTENQQLFSSNSEGLNDIPLNSDFSAYQFRKSMENGRPDYVDLENNDNVVRSRRSDSFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL54</accession>
    <name>A0A1E3NL54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_72900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017980.1">
        <property type="nucleotide sequence ID" value="XM_019164324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL54"/>
    <dbReference type="STRING" id="763406.A0A1E3NL54"/>
    <dbReference type="GeneID" id="30181011"/>
    <dbReference type="OrthoDB" id="860at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF93">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 25D9-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="243" mass="27188" checksum="DF7C682C5DAD1A6B" modified="2017-01-18" version="1">MSATKDAIARCNSRYCEQLQARPLTTKAVTLAFFALLNEQLASFFAGDIKQLRLQIPGSKRQLAVPHTVTSRVPLMGLFALLVNAPLTHYGYRIIQKLVPSPLTPRKRLLQILLSTGIITPVFCACFVSWVGLINNLAKIRDRFQNDKTACLLARVHHTLGFVSTIIASSLKASFVKVASTSVVTSPVFMVLAQKFVIPEGWAVFFAFCYFIVGTYNNTRVKLEQKRQQKKDQHTEPSEKKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL59</accession>
    <name>A0A1E3NL59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger C3HC4 RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_137959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017982.1">
        <property type="nucleotide sequence ID" value="XM_019160113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL59"/>
    <dbReference type="STRING" id="763406.A0A1E3NL59"/>
    <dbReference type="GeneID" id="30176800"/>
    <dbReference type="OrthoDB" id="4090114at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038886">
        <property type="entry name" value="E3_SLX5/Rfp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ46869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="67517" checksum="BBC525013849CA05" modified="2017-01-18" version="1">MLSRSSTLENQGQDSHGNGGVVDNDDNDDIQITSASSANTGIAEQLQNQHSRQRRRLRTENERQRLRQQRQQRRNRHGDGPRTSTSTGTSQGANATAGSGASSNIDDPIVIDLHASQEAVSGFYTNPNTHIQDDGPILITSDAEDLESIERNGLDDPGFHSDTEVSGSTSPRVRMALSADQTSIRNALSAQQQHLQHLINQQQQRENAPSTHEMDDDIQFVGERELPDLQILRSAPGYELHTPSGPLFVPTTTSSEIRSAGNRSRATMGRFGARTPGSSGFLGLVRSNRRATPSQHTLRQRQLEANRRARDAAGNTVADRVRRRPQRSSVRAGHELQNVTVNGRPRNHPILSLEGLQAYSNMYRFLHSLGFRDDSTVFQDVGGFPWISGGGDGGGNGEMENGDGSNVPANVMRILQNRDEERENQRIQMRNTIATTEKNKKAKEARVDLRQRMKFSNNLGEQGQTDVCLLCGVTLLQGIPENCFGEGAEDREKAVKRLVREGFISPWKAWTRFTKIEVDLSKKVFFGTCGHIYCGRCVNNIMKFRGLTARQRKDELNKTKKDPAASLDRVSAWDQNFENPAFAAPLKCIADGCTRRFTGKTPFNELYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLM4</accession>
    <name>A0A1E3NLM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YEATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15049</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018158.1">
        <property type="nucleotide sequence ID" value="XM_019160642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLM4"/>
    <dbReference type="STRING" id="763406.A0A1E3NLM4"/>
    <dbReference type="GeneID" id="30177329"/>
    <dbReference type="OrthoDB" id="1741717at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16905">
        <property type="entry name" value="YEATS_Taf14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016665">
        <property type="entry name" value="Sas5/TAF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016551">
        <property type="entry name" value="SAS5/TFIID_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YEATS" evidence="4">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="273" mass="29580" checksum="159EDFC11C8A1C25" modified="2017-01-18" version="1">MVEVKRTVRFTTVQHVLKDIPAVQEGFPMREWSISVSLVNDKGVEVPATIFDKVTYSLHPTFANPVRSFKKPPFTIQEQGWGGFDIPITLTLIEKGGDKKINHDLNFYKEKYVIDKQIVINTTKPNLLKELAKSGSVPSAGAEVTNGSADSAAAAAPATAVASTGVSTGATPGAVSAEKRKGAAGAAASEPKNVKRAKTTANKGGVDLEKMADLLTKLKEDDLLGVVQMINDNRTPEMNIKNDVDNGEFTMDMYTLPDTLLKSLWDYVKKRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLP3</accession>
    <name>A0A1E3NLP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_16367</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017601.1">
        <property type="nucleotide sequence ID" value="XM_019161182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLP3"/>
    <dbReference type="STRING" id="763406.A0A1E3NLP3"/>
    <dbReference type="GeneID" id="30177869"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF36">
        <property type="entry name" value="PROLINE-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="77"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ46488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="583" mass="64049" checksum="A87C4110151F9223" modified="2017-01-18" version="1">MNRLKDVFGTSGPVPESTVTTSDGEVYETQIGSATSKTEKNLYEFQVDHDEVDDSENSLMGTQQKGTEMKQGLKSRHIQIIALAGAIGTGLFVGSGSALASCGPAGLFVGYLVLSLLVWMVMNQIAEMVTFIPVPGQTTVYALTQRYTGNPSLAFTAGLNLYYAQALIAPSEVTAAAFVIQYWTPINSGAWITIFWVTMVALEFCAVQYFGEVEFWIGLIKIFTITGLIILGIVIFFGGAPASHGVLGFHYWKHPGAFTEHLVKGNTGKFLACWTSIIKSAFAFILSPELITACASEAKNPRVNLPKATSRFIYRMIFFYVFGSLTIGVIVGSNDPRLMSAISAGASSAAASPFVIGIQNAGIKVLNHIVNAAILTSAYSCGNSQFFSATRTLHSMAVRGEVPRIFGRTNRYGVPVYSVINTALISLVSYLNVSNSSAVVFTWLTNISTVSGFFSWVFIGIAYLRYRKAIDYHNLNDRVTFRPKFGIAGAYLTIFFFTLISFTNGYAVFFKFNASDFVAAYITIPIILVLYVGHMLWYRNFTLFAPPEQIDCITGLKEVEQQEAEYEAPVAKNWLMKVYFWLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLS6</accession>
    <name>A0A1E3NLS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_106839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018190.1">
        <property type="nucleotide sequence ID" value="XM_019159706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLS6"/>
    <dbReference type="GeneID" id="30176393"/>
    <dbReference type="OrthoDB" id="10348952at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="269" mass="30588" checksum="2895C908894C5DDD" modified="2017-01-18" version="1">MKMSHQVTLESNSDSISTKHTDNTNSCYYDEQSCIDKVEENKKEIHGNEWEELGESKTYSLIQSMLMTESPSWLAESTLFRKPVLENSDILHHYLTGENEYNLSNKQFTDQPLIETTLEDKTRSYVNLAGLGSINSDNKISLSNQLVEEKPLSAFRGFFDKCLGKIFGKGNIKANKSQQTFDVSNRVEESEASDISDNPIFSLDLDSNTYTNFSNLSLSSRVDVPNDFESFVSDDQWLFNVVSAQKFKYLCTNCSKHCWEESGKVPNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM21</accession>
    <name>A0A1E3NM21_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="11">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="11">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COQ6</name>
        <name evidence="13" type="ORF">PICMEDRAFT_33394</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="11"/>
    <dbReference type="EC" id="1.14.15.46" evidence="11"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017741.1">
        <property type="nucleotide sequence ID" value="XM_019162820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM21"/>
    <dbReference type="STRING" id="763406.A0A1E3NM21"/>
    <dbReference type="GeneID" id="30179507"/>
    <dbReference type="OrthoDB" id="683240at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120538">
        <property type="term" value="F:2-methoxy-6-polyprenolphenol 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106364">
        <property type="term" value="F:4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="21"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="342"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ46628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="469" mass="52180" checksum="6D15C835D3CCC760" modified="2017-01-18" version="1">MFRRFLATAAKGSAGPTIEAADIVIIGGGPAGLSLASAVKASPVLQGLKCTLVEGGKLVESLEKFYESPPQFIDKRVVSITPASMDFFKQIGSWDYVKKDRTETYDNIHTYDGVSGAKMEFDSPELATMIENINIQSSLLQRVKQLNNENPDNPLTILDSTKVVDITEDPQNEWPILKLDNGNSIRTRLLVGCDGYNSPARKFAKIESRGWAYNRWGNVATCKYKDNEFRFPTGWQRFLPTGTLAFLPLPNNWCSLVWAVPPEISAIVGKLSDEQFTYMLNAAAKLTPDELTYLYEIADKEPENLIEEIKWRLGLFNKTLTTVEQKENFPLEIDYIVPNSRAKFPLKLSNADSYVEERVALVGDAAHTTNPLAGQGLNMGQADVKSLVETLENSCKRGLDIGTKIALEPYWAERAIPNHVMLGIVDKIHKIYSTDFAPIVWARSLGVNVLNSLPFVKDFIVGEISHKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMJ2</accession>
    <name>A0A1E3NMJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_16454</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017716.1">
        <property type="nucleotide sequence ID" value="XM_019161209.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NMJ2"/>
    <dbReference type="GeneID" id="30177896"/>
    <dbReference type="OrthoDB" id="3998257at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133439" description="Flo11 domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="763"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1101"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1178"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ46603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1290" mass="133330" checksum="370043D51A1D3FB8" modified="2017-01-18" version="1">MIFSIVFKLVIFSALSTAELLFQNSSSSLISSTKPATVEPSALAISVNSQNEVQIDIPTSYNPLQYATILQDQLASSFVWNEAGSSFFQCSSDSSCTEFERSKVDYSRETTFQVDSTLNASPDPGKFVRFISPSISYPSNGIFPVTFDVILMTDDGMKIFTLQATIYQMSTTSSDQYTWIPTTICQYDHCVTTSVPEIKETQTTVIDGLTTTYETYTPVSIATKSVQLYLTTTVLVTSGSSITIVTYCPLTDDTLTTSSSPLSSTSSIPPTPSAPSSIHLTTSFSTLKYSSTPSSSSHLLSESSPVAVVSSSSLADLSSTKLSTSSIYPSSSLLSTASSQKFLSSMSTSITVSSTLIPSKPSLPTSYKSSVLHSSLSSLSTSLSSSMISSVPSSLQSFTFSSSSKSSNSLTPSTFPSLISLSSSSIQKMGSLSSTLQTSSDTTLPSKSCSSYSTITITSTTTECRAGSCATSTLINTVAYCPTTSSFVSRFSDSSSYSTSTTLAPSTFITDCLGSSRKVLCDVGVTTFLLGETNVVLPTTSCQIPHSVTGSYTNIENLYTYSNGITTTVLNFPKLIFPSVVCPVSRFVSSVPSTASSAITIPSLVPSSSTKTFVVTTTSCNSFTCDTSVITKTTTYCPLSSLLTSVSSKTSSTVSSLFSETISSVHAVPSSDISSAYYSITSSSSNPSASATSFPTASITSNRSIESTKTKDTSTSSSSASSLTTEVTTVTTTSCNSESCTESVITKTTTYCPLTDSSTSFTSKTSATTMSNPTLSTSASDSTSKLVSNSATSLLSSTLVTPSTSVTTVSVSSAPSDTSFASNPSVFTTSPIVTTLPSSITGPTSSTSSSLTTQVTTFTTTSCDFGTCSTSIVTITTTYCPLTSLSTTSATTSATTSATTSATTSATTSATINSATTPASSFSSTLTTISSSEISQDSTHSTPSFSSSTTLVVTPSASSLEPTSFTETSLSSSSSSFTTEIKTLTTTTCSSGNCYTSLTTVTTTYCPLSPVSTTLSSSEFFVLTTSSVDSNTIFSTAITTASPSSLPSSTTDVITSTITSCNKESCSSLIVTLTTTYCPLSSQGNPTETTEYELSSQGVSLSSSGIQSTSETETTLPSSTVSKSTDYASSTIVVSIKCTKDVCSAVTQTTGITTVTTVQSGISTVFTTFCPLSSTVSTLSTSPSPSSESSSAVLNTQTTQNSGTSTTTIQLTTLITKSCSDSTCTTFEVISTATNNKTLPSSVSVPTNVTAPVVSSAATAAPFENRAIRLGSVHGGGIMATVISLGMILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMM2</accession>
    <name>A0A1E3NMM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_16676</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017981.1">
        <property type="nucleotide sequence ID" value="XM_019161303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMM2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMM2"/>
    <dbReference type="GeneID" id="30177990"/>
    <dbReference type="OrthoDB" id="1859733at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000056">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000001">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ46868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="313" mass="33151" checksum="52FDE705A6DC14FA" modified="2017-01-18" version="1">MAFVQRGGRGGSRGGRGGSSRGGSRGGFSRGGGRGGFSRGGGRGAPRGGARGGRGAPRGGRGGARGGASGGAKVVIEPHRHDGVYIARGKEDLLVTKNVAPGESVYGEKRVTVEEPSKDPSVPATKVEYRVWNPFRSKLAAGIMGGLDQIFIKPGAKVLYLGAASGTSVSHVSDIVGPEGLVYAVEFSHRPGRELISMAKKRPNVIPIVEDARHPQKYRMLVGMVDVVFADVAQPDQARIIALNSHMFLKDGGGVVISIKANCIDSTVDAETVFAREVQKMREERIKPLEQLTLEPYERDHCIVVGVYKRSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMN7</accession>
    <name>A0A1E3NMN7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018492.1">
        <property type="nucleotide sequence ID" value="XM_019160778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMN7"/>
    <dbReference type="STRING" id="763406.A0A1E3NMN7"/>
    <dbReference type="GeneID" id="30177465"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000004">
        <property type="entry name" value="rRNA-processing protein FCF1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="62"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="188" mass="21629" checksum="DFCE4DEB76918AC5" modified="2017-01-18" version="1">MGKAKKTRKISVKRMVKADDQRIKKNQDKTKEPEEDKELIRSVPQVSSALFFQYNQSIKPPYQIIIDTNFFNFSIQKKIDIVRGMMDCLLAKCIPMVTDCVLAELEKLGHRYRIALTLAKDPRIQRLKCSHKGTYADDCIVSRVIQNKCYIVATNDADLKRRIRKVPGIPVMSVGGHSYLIERLPDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMP2</accession>
    <name>A0A1E3NMP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_178225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018484.1">
        <property type="nucleotide sequence ID" value="XM_019161763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMP2"/>
    <dbReference type="STRING" id="763406.A0A1E3NMP2"/>
    <dbReference type="GeneID" id="30178450"/>
    <dbReference type="OrthoDB" id="7763451at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF10">
        <property type="entry name" value="RNA-BINDING REGION RNP-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="281" mass="30123" checksum="7FB9EE65E26C11B7" modified="2017-01-18" version="1">MATITASQIPVSVPENKIKEFFSFCGKIKDIEVLDKTEKTKTIRVEFEKASAVSTALLLNGAEFEGSQIEVKEDSSSGAAAGASAGEKDVAGESGKGDSDIDQESKPKSTIIAELLANGYVLQSSLVEKAVDFDQQHGITDRFRDFISGLDQKYHLQEKNQQIANTQWGLESHWNQGVRTLNSYLDRFKKDKYGSQVHDFYTNAAKDVKSVNDEALRLAELKKKQHEGADAGAGTDADAGAASSGAPKTGAPTTVFPAINTITPDSSSAAAASFAPLEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMQ0</accession>
    <name>A0A1E3NMQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018510.1">
        <property type="nucleotide sequence ID" value="XM_019163934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMQ0"/>
    <dbReference type="STRING" id="763406.A0A1E3NMQ0"/>
    <dbReference type="GeneID" id="30180621"/>
    <dbReference type="OrthoDB" id="6021263at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024388">
        <property type="entry name" value="Ribosomal_mL58"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12824">
        <property type="entry name" value="MRP-L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="192" mass="21575" checksum="D299AA50BE458669" modified="2017-01-18" version="1">MFQCRAALSKCKGFSVRFNSSSSSGIPKLGTTPNKFNAKSSAFNLRPELPEGLFFHPAPAAPNPEITPKAFLPKSDARAASDLYYPEQDSLVTANVAYMPVVSKASQPKNYKFDASIVEQLQTLRDQGVSRKQIREEFGVTDNFISLVTKPNAQTQQQQKKLLKRSARKWSAKTLKARRLKEVKKSHWERDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMX8</accession>
    <name>A0A1E3NMX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thymidylate synthase</fullName>
            <ecNumber evidence="2">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_71543</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="2"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018585.1">
        <property type="nucleotide sequence ID" value="XM_019163949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMX8"/>
    <dbReference type="STRING" id="763406.A0A1E3NMX8"/>
    <dbReference type="GeneID" id="30180636"/>
    <dbReference type="OrthoDB" id="766at2759"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000002">
        <property type="entry name" value="Possible thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002497">
        <property type="entry name" value="PRK01827.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="8">
        <location>
            <begin position="15"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ47472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="304" mass="34722" checksum="D494E9EF3E9E4720" modified="2017-01-18" version="1">MAESLSTDGRNLEEQAYLDLLQRIIDDGEYRPDRTGTGTYSLFAPPQLRFDLSDGAFPLLTTKKVFMKGILHELLWFVHGSTDAKLLSAKGVKIWEGNGSREYLDSLGLTSRREGDLGPVYGFQWRHFGAEYKDCDADYSGQGYDQLASIVHTLKTNPYDRRIIMSAWNPPAFKEMALPPCHVFCQFYVSFPKSGRPRLSCQMYQRSCDMGLGVPFNIASYALLTYMIAMVVDMEPGEFIHVMGDAHVYSNHVEALKEQLQRVPRKFPKLVIKRKVTDIDDFKYEDFEVTDYDPYGPIKMKMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMY9</accession>
    <name>A0A1E3NMY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_114088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018598.1">
        <property type="nucleotide sequence ID" value="XM_019159788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMY9"/>
    <dbReference type="GeneID" id="30176475"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="121" mass="12959" checksum="1640247E7042BA0D" modified="2017-01-18" version="1">MTTSRRPRQTQGLVLHKGQLERSHSLAGRASQTWKQTVRCGCTSKVRAGRRSITVFPSIGSCLARNQALSAATCYLWSVSLTVLVEAATVATAVVAADPARLSAYRHPRRPNPPSAASISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN52</accession>
    <name>A0A1E3NN52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_31833</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018647.1">
        <property type="nucleotide sequence ID" value="XM_019162726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN52"/>
    <dbReference type="STRING" id="763406.A0A1E3NN52"/>
    <dbReference type="GeneID" id="30179413"/>
    <dbReference type="OrthoDB" id="18996at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000003">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="99"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ47534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="205" mass="23774" checksum="DF3786C78F67D349" modified="2017-01-18" version="1" fragment="single">MSIPLREGFVDETLERCLEDLIVRFVVNCPDEDLSSIERVLFQIEEAHWFYQDFLRTSNPLLPSMKMKQFTNRLIEQCPLIWKWGDPSEALAQFGKYKSTIPVRGCALLNSSMDKLLLVKGIESPSWGFPRGKISKGEKDLDCALRELEEETGFDASNLIDEDEFVERTIKGKNYKIFIIKGVPDDTNFLPKVRYEIVDIQWMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN58</accession>
    <name>A0A1E3NN58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translationally-controlled tumor protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_32463</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in protein synthesis. Involved in microtubule stabilization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TCTP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018660.1">
        <property type="nucleotide sequence ID" value="XM_019162768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN58"/>
    <dbReference type="STRING" id="763406.A0A1E3NN58"/>
    <dbReference type="GeneID" id="30179455"/>
    <dbReference type="OrthoDB" id="10248936at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.10:FF:000002">
        <property type="entry name" value="Translationally-controlled tumor protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018103">
        <property type="entry name" value="Translation_control_tumour_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01003">
        <property type="entry name" value="TCTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="5">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="168" mass="18759" checksum="28A274D65FBA44AC" modified="2017-01-18" version="1">MIIFKDLFSGDELLSDAYDVKLVDGVVYEADCDLVQVRPGADVDIGANPSADGGDEDVEEGVETVNNVVYSFRLQQTGFDKKSFLTYIKAYMKAVKAKLAETKPEEVEVFEKGAAAYVKKVIGSFKDWEFYTGESMDPDAMIVLMNYREDGETPFVAIWKHGVNEEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNK4</accession>
    <name>A0A1E3NNK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_26722</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018787.1">
        <property type="nucleotide sequence ID" value="XM_019162460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNK4"/>
    <dbReference type="STRING" id="763406.A0A1E3NNK4"/>
    <dbReference type="GeneID" id="30179147"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Deprotonates C-terminal active site Cys" evidence="2">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="2">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="2">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="3">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ47674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="99" mass="10776" checksum="CCB09B21EBB6727F" modified="2017-01-18" version="1" fragment="single">MVTVIQSEDEFKTAIAAQHLVVVDFFAVWCGPCKMIAPMLEKFSNDYSSAKFYKVDVDQLPSVAASNEVTSMPTLLFFKSGELVGKVIGANPAAIKQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNQ2</accession>
    <name>A0A1E3NNQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_71261</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018266.1">
        <property type="nucleotide sequence ID" value="XM_019163876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNQ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNQ2"/>
    <dbReference type="GeneID" id="30180563"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="91"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="243"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="555" mass="60784" checksum="DD16EBB8953BB47D" modified="2017-01-18" version="1">MTFDKTELKVNPENEELYKTFLPKVEKYRQALKDGILEEYKLPDEFLPKNVGVDVTKIPEQILSKKEIYITELKAVDLVSQIAKGELSSVEVFKAYAKRATMAHQLTNCAMELFTDEGLKRAGELDEYFSANGKTVGALHGVPVSLKEHYDYKGKITHAGYVGLIDNVSESTNQCVQILYDAGAVFYVRTTEPQLLMHLCSNNNITGIAKNPTNTALTTGGSSSGEGAIAAMKGSALGVGSDIGGSIRAPAAFCGVWGLRPTQKRIPMLPITPAGSGVQELVVPVLGPLARSAEDLELFMKVELGSKPWETDATLVPMPWRDVAVPEPKQLKVAVCYDDGVVKPTPPILRGLEAAAKKLKEAGVKVVEWKAFNVQECVTAAYDGYNADANKKQLSLLKLSGEPVLPLSKHILNSGCGFDGLSVYQYQNDSGVRDGTRTAYLEQMNELDVDFILCPVYSSVAPLPETCHYWGYTSLWNLLDFPNVIFPTGLKCDPAVDKPDTSYVPRNDTEKYEHELYDDAAKFAGAPISLQLTGKRWFDEELVKASQIVAEIIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNU2</accession>
    <name>A0A1E3NNU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_10346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018461.1">
        <property type="nucleotide sequence ID" value="XM_019159661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNU2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNU2"/>
    <dbReference type="GeneID" id="30176348"/>
    <dbReference type="OrthoDB" id="440385at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="115"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="261" mass="27884" checksum="D1BA0E0DFA492982" modified="2017-01-18" version="1">MSYYQQYSTNNAQGSAFLGSNNQNLHNANSSMNTGYGYGMATAGQGFAMPSGSMASVNTKAADYDAPLPEGILAAFSTSGYPGEAPLFEELGVNFSHIKAKTMAVLGMRSTSLSEDVIQDSDMAGPILFCLLFGTLLLLSGKTHFGYIYGVALFGTVSLHWLFKLMSSALDGGLDFLRTASVIGYCLLPLVLLSALAILVQLNNVIGYSLAVVCVSWCTWAASGFFVRVLNLSNARPLVAYPLGMFYSVFALMTIFAEKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP34</accession>
    <name>A0A1E3NP34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein ZIP4 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_10803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018968.1">
        <property type="nucleotide sequence ID" value="XM_019159718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP34"/>
    <dbReference type="STRING" id="763406.A0A1E3NP34"/>
    <dbReference type="GeneID" id="30176405"/>
    <dbReference type="OrthoDB" id="3995209at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090173">
        <property type="term" value="P:regulation of synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039057">
        <property type="entry name" value="Spo22/ZIP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013940">
        <property type="entry name" value="Spo22/ZIP4/TEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40375">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40375:SF2">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08631">
        <property type="entry name" value="SPO22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1005" mass="116280" checksum="F08B5B1FF187C594" modified="2017-01-18" version="1">MQAPEISLKVVSTLTELIASCETNIKILSGISESDDATFDLACIQNRLNSLIPMLESTEKVIRMEYLPLLSKSKEKLSPSIKKILPSLEEVSLNFWNALNIKQKESEEPEEDGSTVKTWKLEFKKMMSLKYFVSLLLSLFALLNFLNDKVKIRKTGKQVKKYVESLCAYYDNNKLGKEEKNKYETLKVEFLIMDMQSSLLSKNISMAKFYEHKANILDRCSAMKPGNVFSICRTLYNEGLRLYKEEQYHDSHYFLQKCYLILEKMDTFKDPSVESEIKVSTLIMLSKCCMKMNSKESFTEASKLLKLLQLNENEKIETLQLQIELIDYQKLANNDAEDLIMKTVIKICSKTEILKQVMVVLNAYSSKCPTTAKNCLFYVFTNKIDFENPKFREVIESYLISLIWIITSQLKTENSSEKLNAVKTILEVGNRKLRFELSIDSANCLVIMLWSMGRKNMKEENYQEAMSWFECCFVSLLNRSKESQQDTIGKIQRSILHCCLKMHDTGTFENTLRDMNDLSRKNPITLYYRFVNMLENHGTEIDDDTAHEILTALSRCNDLKTINMLALCVVESKNYLDSNCHDRDKPFLDEPLKHAVSKLLNKCNNIDTKNSEYLVLVAMRASVYIFGKRLENGLKKDETEKMSLENVEMIEKSVDQFLCYAKVNREGSHIKDINNDIEWFSSTCFNCGLLLLENNIFDTRGVKLFEATIRLIELFNESDDKNNDRFLKWKCKSIIFLSFCEREQIRQGGNNVKQKWNAIRVMNQQVVEQISLIHKDNDYKDIMLQGLYLINESLINCNEWTSLIKRIKDMNEYGKNHTEDNQQIDAMVENIFNWIPDGDAEQRGGMIRVIETIFAERGFKNEFAVRTRMLFRWLHLMLTKMTENVAFEDQQLCYIRMFRDILAETPTDGADRLKDYEIEWLAGVCWNKGIETILRCKAAIEAADVEDVGMDDSYADEDKEFFIAAVADPANSMLGGKGLPWCEIAIEITVPGSCNALECRTCSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP76</accession>
    <name>A0A1E3NP76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bud emergence protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_57954</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018611.1">
        <property type="nucleotide sequence ID" value="XM_019163268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP76"/>
    <dbReference type="STRING" id="763406.A0A1E3NP76"/>
    <dbReference type="GeneID" id="30179955"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06890">
        <property type="entry name" value="PX_Bem1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035550">
        <property type="entry name" value="Bem1/Scd2_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="37"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="172"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="6">
        <location>
            <begin position="311"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="543"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="279"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="460"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="543"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="76095" checksum="D9A4B7B2D11FE29D" modified="2017-01-18" version="1">MASIFKKAKDRSSKEKLRTTSGTSLYASNDPLNPLNAPTEVAQATSFHRARNSNELSFKRGDFFHVISAVQASDGTIEIFDPVKNVKGRAPAACFKIFEKTHHANGSGDGENRISGSSTSSGRMNSNSNSMGHKFASNGSQFSQHEFSQPRHTSTTSSSSFSSYGNKKESALGNLFGVVLYDFNAERSDELSVAAGDSIFICAHHEYEWYIAKFLNKVGEVGLVPVSYVQLIDAVTRIPYKGTPRSIIENENLPTVEEWKALKNKHKASSRTVGMHDNTVPSNGALSRQSSQGYVRSKSITKSPLNKNISESLVLPDNTNIESFSSTNNKFWFLIRVGMSDGSIRSLCRYYEDFFNFHQKLLSTWPREGGKFDTKDKKERILPFIPGPVIDVTESLCHKRLIDLNEYLKILLQLPDYISKSNLVVSFFDLMDGDETSNNTLSKTSYVPVEPNRPAPNLKIINSDNSHRPSSVSPSQQQQQQQQQHLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQKAQQGSIQPTETLSQSHYDRKSQYELNNRSSSLSNKVGDFTSTRKSSTNSVVSQGATVSQTPPTSKNSSSLKLKLKFYYKDDIFAISVPNDIKLSVLKALVVPKIDECDSPGVESKIEIYSKNSSNVDHSTYDKSKALRNDNDLWNDANFVDKGKFLIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPE9</accession>
    <name>A0A1E3NPE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_15849</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019104.1">
        <property type="nucleotide sequence ID" value="XM_019160995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPE9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPE9"/>
    <dbReference type="GeneID" id="30177682"/>
    <dbReference type="OrthoDB" id="5382797at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="7">
        <location>
            <begin position="318"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="446"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ47991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="768" mass="85790" checksum="5B6A15E8C85C8A04" modified="2017-01-18" version="1">MSHKVPPQSAPTAFSSPSASSYKRRSLQSPFILNDLAGDPDQEKSQPKRGSDHDSKSEEEKELEEREDSMNVFGANSYSTKDEDLVIHRGGSDDNTGPVGTPTPTNETFLYDFPQPLRTDSPNLPSQRYSMQSLSSVTSNLNPSLTTSTSTRKHNRSRSTASATYTPTNIPPSKPFSRQDTPPLTDSNGVFAGITNSPLRSPAHQPFKFNSMIMNNSSENLAQPVSSSPQILPKPSYRRGHRYKHSSVSMNMFQDPQRKASTNKPHILPKKYTIPTFNEVTSMITSSQKSKLSVCLIQGFFVLLAYIAGFHYSNGCLSTLAHILFYDVISNFSSIAVQIMSNFEVWRVSSLKYPFGLGRIEVLIGFALSVSLLFVGLDLFSHIVEEMMMSSFENDNENAINSQGHSHGHSNGESPHHLHPVLYEIFILGTIVTTLLTSHIVNDTTTSEKKEEHEELPNRPESKIAPNVKRLSSITLNEPAREGVFNRLGIYIKSKLGLKPNLFHSSTTNLSVLYAIYCLYYPFAQGILSLHTGNTLLSVIGLSSKDEHMIDSEHKHDEHEYEHELLNNDALEATKWINEVSTVIMAILVSVVAWRLIWRLGNILLLTSPSIAFDKKNKIATASAEVDRIGNHSDVESLIVSNVRQLDVYKNSYSIEEVKIARVNTRVYVVIIRILMPGASDDEESKFRFYTMRIVRGIMYKCVKGQLKKRKGFLGDGSSDTRQNTESIAEENRRSLIDLLNLSTSIEDVEGIDKSGDQFEITIDVGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPH7</accession>
    <name>A0A1E3NPH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_19001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019127.1">
        <property type="nucleotide sequence ID" value="XM_019162101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPH7"/>
    <dbReference type="GeneID" id="30178788"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19677">
        <property type="entry name" value="UBR-box_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040204">
        <property type="entry name" value="UBR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047506">
        <property type="entry name" value="UBR7-like_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="6">
        <location>
            <begin position="22"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="4">
        <location>
            <begin position="22"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="396" mass="45330" checksum="4D0A5E92104F3236" modified="2017-01-18" version="1" fragment="single">EYLEDQQALEKQAREQMPYDPNECTFAMGALRQELYACLTCYRQTAQLNAVCYACSIRCHTSHDLVELFTKRSRTCDCGTSRVSGPCLVRYPQWTKLDAEFQSENDSHRFTPDIPDSNNRYGHNFKGEFCDCNMPYNPLTDSNMHQCTFGVKCDEDWYHEECIMGLRPGVVNRSPKRAARSQAIHGRDRLDELSEPGLDASSDNKVKAEVDDEDEHDDNDEEYDILPLPGFPDLDSFDSTICWKCASEYPAEMEQLVNTLHCETVISMISPDVSTTDEKASKRTKKDFPKTIFLKEDYREKLKHHVDTEPSSKLSVLLKKYPFLYLDDPIYRPPDDDDDASSVFELGIRELGNMPSDQAAQGLAAYEKIKSKLTEFLKPFAQEGKIVTEDEVKAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPN2</accession>
    <name>A0A1E3NPN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_122937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019194.1">
        <property type="nucleotide sequence ID" value="XM_019159910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPN2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPN2"/>
    <dbReference type="GeneID" id="30176597"/>
    <dbReference type="OrthoDB" id="3996380at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22092" checksum="4B736247DAAC489C" modified="2017-01-18" version="1">MKIKFVSLISRDNRPLYIQSFVSTQNEKSPSSDATTPEEMNELLKYNFLSHMSLDVFESPFAPIGTHSAKGKPTLLFVQDGVLVYGSETLTGLKVVVGCSRYNREAEKGTGKEPEAEEGSTTVAAKVSSEKSLGNLDGFVDTDVIELSDLDSVTTAIRKEYLRYSCNPFLDPKEETEINSEKFDRQIKRIVDGFNSESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPV3</accession>
    <name>A0A1E3NPV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Genetic interactor of prohibitins 3, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Found in mitochondrial proteome protein 38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_15909</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019179.1">
        <property type="nucleotide sequence ID" value="XM_019161018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPV3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPV3"/>
    <dbReference type="GeneID" id="30177705"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050896">
        <property type="entry name" value="Mito_lipid_metab_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434:SF1">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CP-type G" evidence="5">
        <location>
            <begin position="210"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="650" mass="74345" checksum="559C0CB4F225C829" modified="2017-01-18" version="1">MRRVLFSIKTTGKPGRLGFKANPFPQYDFLRSNIPAFSFSTSNMNKSEPKPKVELPIFSSEYYTKTKAHAVMLNQEKVKHEYDLKNAYDEYLSFLKSREISEKLKCKSCGTGPLQSNDSGSDKYYKPPKFSAQIGFKKNIGHLQKNQLFTEAELALKKEMGIYDEASNLAEEKEIVFEQKVKQLAELANSKVECLRCQQLNSHGVFDIFGQQIDQVLEKIPSNGKIVNIVSILDFPLSCSKGIIKGRDPKDIWHVVTKADLFFRQEVQLNRTGLQYVQEVLEKYLDADPEKVFFVSTTKSWNNEELLQKLPGGDLYFVGRANAGKSSLIKSLIASTHGVDTQNVLAKKIEKKSMNYLEKLGLDSPGTSHIPGYTRDFQKFKINDRITVYDTPGIFPNDLGFYKYLTEKLARKQPKYTAFVAEDKKRYKKLDVKGPKVFSGKSLYSYGGFFYLQPPKGAIFKRCLAFKLGEKTFEARYSKLGRAEEINETRPKQIGNRFGVTKEAFYNLKRYVIPPFYGTVDIVIQDLGFLSIKMTSSPDDVDGLFQIWVPEGVRVIVRESIFNFLYKSHDLVDETGNKLKKENISKRGATRLRAIFDEDKLHFTELVQVPVNESDEQAFLSVCPPGDMKISNTADSQEEYKNQYWRKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPY3</accession>
    <name>A0A1E3NPY3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_58459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019287.1">
        <property type="nucleotide sequence ID" value="XM_019163300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPY3"/>
    <dbReference type="GeneID" id="30179987"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="64" mass="6940" checksum="333E9699C4F70048" modified="2017-01-18" version="1">MTSWLVPPSGFKVSAICTELGPCIAWKGLFRGQGPRSESTGPSFTRIAMLHPQPRVLRSRPAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ13</accession>
    <name>A0A1E3NQ13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_26145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019268.1">
        <property type="nucleotide sequence ID" value="XM_019162435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ13"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ13"/>
    <dbReference type="GeneID" id="30179122"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000016">
        <property type="entry name" value="mRNA cleavage factor complex component Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="3"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="605" mass="67655" checksum="140BA52D2546634B" modified="2017-01-18" version="1" fragment="single">DVTADQFAEDYYNSLQSLTFNSRPIIESHTNLAQENSTFAPQITAAIEKRIDKAIPSQKLFSLYLLDSISKNIGLPYTSLFSNNIFKTFTRTYGLVDDQTRCKLIKLFKTWKLPTALTGLPLFDSNQLDKIEQFLMKVTAASNPGINYVSSLNSSNSNSNSNSNSNASTPNSTPMSMNMAVNLKPNLIKETDDLLALVSSRLKSVPSDEKGQQRHKLLNQLKSILSSNSAIPKSQLDEVAKQLQSIREDEILKLNILRQQQLQQQQQQQQQQQQQQQQKSNFSASQLQSLLNFGKDNNQTSGVPPNNAASAQALFNMMSSVIGKSPPASGSNAQAINNINNNNSSSNGSSNGNNNSNNMTNPLGLKDISFLQNMLKKTSSQQTQSSSPSGPQVDFKFIRPTKEAIIEDFTLTQSMISNHNPTQKEISLLYDFKPNQCTRCSKRFAASPEGDSLMRKHNEWHDRVEQRLTMGGSGLGGVVNRSWYLTESAWVEFDDYRESDETKKDARLALASTSVSVTTDRNDAGRDYRDRDEKIDLSDAPNHTVTIPESSSNEVVCGICREKLVGAFDEDSGDWIWKNAVEQKGRVWHWTCWEEAKRTSRRDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ69</accession>
    <name>A0A1E3NQ69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_15999</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019299.1">
        <property type="nucleotide sequence ID" value="XM_019161049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ69"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ69"/>
    <dbReference type="GeneID" id="30177736"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="264" mass="28467" checksum="316D0F21437E3E68" modified="2017-01-18" version="1">MNMSGMDMSGMDMGSTTSSSAMQMTMTHSSASATSTGMDMSGMTMSMSSASMAGMPSMSMDMSGTATSATASATSTMDMSGMDMGAGNKSSMSMTMWMTTKYQNTPVFFRSLDAHSGAAAFGIFCVLFFCSFFFRGLIFLSSYLEQAVFHNYSNTIIVEEDCDCGPADDKASPAPTVPNSPPFGEVMKKLFWMGPRELMNDVIRLLLAFTIVMFGYAIMLAAMSFVILYFFAICLGLSFGEIFFNRLSIILNINKSFGACSGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQA4</accession>
    <name>A0A1E3NQA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit BET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_31347</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019339.1">
        <property type="nucleotide sequence ID" value="XM_019162694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQA4"/>
    <dbReference type="STRING" id="763406.A0A1E3NQA4"/>
    <dbReference type="GeneID" id="30179381"/>
    <dbReference type="OrthoDB" id="10262857at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033106">
        <property type="term" value="C:cis-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000001">
        <property type="entry name" value="Trafficking protein particle complex subunit BET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="199" mass="22688" checksum="C978D26DACC9604F" modified="2017-01-18" version="1">MSKQSKHAKQAKQLGEDIWKSQTVKINSELFTLTYGSLVAQICREEKFDYEKINNVLFDMGRNIGVRLIEEFLSKTSLERCSSFKETSEAICKIGFKMFLNVVPNVTYWSADGRTFQLTLLENPLSDFVELPDDIDEGSARKELWYSNILCGVLKGALEMVQLDCQVWFVSDVLRGDASTDIKIRLVQVLKDEIPDGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQF5</accession>
    <name>A0A1E3NQF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAA35-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_69977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019443.1">
        <property type="nucleotide sequence ID" value="XM_019163519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQF5"/>
    <dbReference type="STRING" id="763406.A0A1E3NQF5"/>
    <dbReference type="GeneID" id="30180206"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="1">
        <location>
            <begin position="36"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ48330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="744" mass="86457" checksum="FE16FF96849032AB" modified="2017-01-18" version="1">MADLIPSLESFQISNQKLRDVTDEFFKVTSSIEYSKIVKSNNFSLLHGTHALELLNTRLDTFLLQKAEYSIAKDLDLTEATSIISAQLKCLTCWLGQNVGVPNSLLSSEYISRILESYTKCGNFESIPLRDDSWRTVVFKFSILMISTAKFILNLALKSQIYEDEDINTNTMNLNWFFELSNNDIFKLTLVSNKTWTSLKSQLSTAEDAKYFDFIKNAFQILQTLSSLDSMFQWEIPLFNVNQSHKDFLVKFNNKLVQMEKIVEKLDGIDLLDMDSIQPPVGSFNSNAQIDFDNQSPPKELQILQSSWNDCTLNLTQLFNDLIDVMNILKSKNIIEFVEWLKHLESKRNNTDSNEINGLHIIARIVFYSLINSNQTPDSNPIFNIKNLTFKDLFWTYMKDFALKNSKIDKEMSSNKRNKNVAVLEQVDYYLDAISLFWKESVMLPTLNPSRQRQFKCKELKYWNMRQSETGSLEDYFINLNFYKANEKVYPLTFIIIYFKLKAISEVILKSIELSLFKDVREYVSVYYQLILISSQLNQQISDLILMANTNKNNDSVLYLSFLKNENNLIYQLSILKAKQFEMLSFLGFNTLPPNLIQTPMINPELLYKLQWKQFHNITDPEMLNFKEFQDRLHQSVTLISDVSTFVPILKQEMNGLVNEFTKSANLCNALVGKMRWFEELKDVKALKFDKLLEELAKSKLDTTELLEFVKLKGKDTVTKDYVVNIVRLGRYSCFPGVKLSKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQI0</accession>
    <name>A0A1E3NQI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_13953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019482.1">
        <property type="nucleotide sequence ID" value="XM_019160149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQI0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQI0"/>
    <dbReference type="GeneID" id="30176836"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="40310" checksum="BD88BF5A6418BCAF" modified="2017-01-18" version="1">MARYFSEYLPRLKTTSVQVELPRDQVDSVEVRDDSVVINSSIDIKLPSAIQSSQASHAVNAVSPGLTSIRIRDRQAAFLANDSIESFLQDQYQWNISNIQKYHMHDVLRCVSCDSPIIDLKEVKKLLPMPSELWYEMLDFWHCHKPTVENNNIGLLKKFNQLKPNPKTLIIGSYYFIANPEDWANIEACGQENESLECGCCHEILGELDSNIGNYKILKWQLKVENELFKPFAYISLKLVEEMNYSAVRVFQIIDEKSKRSIQVWCFGLGIDINVDTLGVMKNCLKILYKENMQEDSTSRLKGGNNNSEILVEYEKSFDDFCIWLQKVNQALPENLQKYNDWNVTYIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQN5</accession>
    <name>A0A1E3NQN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_15742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018976.1">
        <property type="nucleotide sequence ID" value="XM_019160944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQN5"/>
    <dbReference type="STRING" id="763406.A0A1E3NQN5"/>
    <dbReference type="GeneID" id="30177631"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031333">
        <property type="term" value="P:negative regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ47863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="256" mass="29698" checksum="542D864F57F4A38E" modified="2017-01-18" version="1">MDPLEEQAQELEILQSIYPDELTILTPIRFQINILLDTDSKRRHAVLLDVTYTENYPETVPILNILEGAVDYDALENEEDDDDEEDGEGNYDDDDDQTISKRLLNLVETIQLDRDDYAELLRKIRDEAEDNVGMPSAFSLASSLKEHAETQFTEKLKSKEKEIMDIREAREREEQKKFTGTKVTPASFENWRQKFRKEMGIDRRLDERYADVHKGRLTGKQIFEQGLASGEDLEDTTDGKEVDMSELKKKLEETTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR19</accession>
    <name>A0A1E3NR19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019645.1">
        <property type="nucleotide sequence ID" value="XM_019160215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR19"/>
    <dbReference type="STRING" id="763406.A0A1E3NR19"/>
    <dbReference type="GeneID" id="30176902"/>
    <dbReference type="OrthoDB" id="346910at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036042">
        <property type="term" value="F:long-chain fatty acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00435">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="2"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="87" mass="9886" checksum="6A51AC5DB4544F71" modified="2017-01-18" version="1">MVSAEFTQKAEAVNKLPTRPSDDELLELYGLYKQATVGDNETEKPGMFDFKGKYKWEAWTKLKGTSQEEAEQKYIALVDELSAKYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NR62</accession>
    <name>A0A1E3NR62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019677.1">
        <property type="nucleotide sequence ID" value="XM_019163584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NR62"/>
    <dbReference type="STRING" id="763406.A0A1E3NR62"/>
    <dbReference type="GeneID" id="30180271"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133468" description="Peptidase A1 domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="76"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="599"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="599"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="611"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="641" mass="67372" checksum="DCF725CC8159E756" modified="2017-01-18" version="1">MVCCTLLTAAAGAGLLAGAAADYVQLQATKVKGSWPQNADVHKFPIYAYYSRSTNGSDAAANGTVAAGVYNDRVYYAVDLALGSEAQNVTVLVDTVSSDLWVNSVDNAVCAAGVDSPSNFTVDYSYTVSGASQSSPALSSTSSSAASSAAPLSTYYTTSYDSASDVFYVDGYVSTVTTTTATEQTSAVTTRSYDVQFYPSAAVASYSQWLSAYPYPTNAINSLVVLEAEPHNCSAWGLFDSSSSESFVTSNDTFESVAEDGTTVSGIWAQDSVAYGNALVSNVSFGLVSNAESDSFGVLGLGLPSAESTWLAENTTYDNFLAKLKAQGAIHKQVYAIYDNYLSGNSSLLFGGIDHEAHVGNLTILPLVEVPIAYNDSRNASAIAITLSAVYLDDESNGANDTSLIASGLGAAIIDTSLATASFPYYLYDEIVAAAGFFYSENLKAFIAKASDIENKTLGLDFQGVEVDVPITDLSFPLVDIVNNKTSDYVVFGAEATANDTVVIGDAVLQYLYFAVDLEDLEIAVAHKNFFPTSQHIVAITSTFPHATSAASYNQTYGYHGVTDLKLATFEDPNSISQTSFSGSFLPSITLYQTTARSTTVAESTTSPEVTSASAKAANTSKSKTSKSKTAKATTTSTTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRC1</accession>
    <name>A0A1E3NRC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin reductase</fullName>
            <ecNumber evidence="8">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_14171</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="8"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019743.1">
        <property type="nucleotide sequence ID" value="XM_019160255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRC1"/>
    <dbReference type="STRING" id="763406.A0A1E3NRC1"/>
    <dbReference type="GeneID" id="30176942"/>
    <dbReference type="OrthoDB" id="371245at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000064">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="10">
        <location>
            <begin position="82"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="397" mass="42696" checksum="C1DEB12227E68EF1" modified="2017-01-18" version="1">MPGGTPGGCWIGMGVYKYTVSGCCFSSFSEAVVPTNVLETASPLSCMHLGHLVNQASLPLLLKRAATRQHPPTSAYRMVHNKVTIIGSGPAAHTAAIYLARAEMNPVLYEGMMANGIAPGGQLTTTTEIENFPGFPDGLTGSDLMDRMRKQSERFGTKIITETISKVDFSSKPFKLWSEFYEDGEPITTDAVVIATGASAKRLGLPGEDTYWQQGISACAVCDGAVPIFRNNPLAVIGGGDSACEEALFLTKFGSKVYMIVRKDHLRASTIMQKRVEKNEKLEILYNTVSLEAKGDGKLLSHLQIKNTETNEVKDLPVNGLFYAIGHTPATQVFKDQLKTDETGYILTKPGTASTSIEGVYAAGDVQDSKYRQAITSAGTGCMAALEAEHYLSSLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRK2</accession>
    <name>A0A1E3NRK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Metal resistance protein YCF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PICMEDRAFT_177992</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019823.1">
        <property type="nucleotide sequence ID" value="XM_019161748.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRK2"/>
    <dbReference type="GeneID" id="30178435"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="928"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="969"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1055"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1159"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="17">
        <location>
            <begin position="281"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="606"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="17">
        <location>
            <begin position="929"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="1249"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="837"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="837"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="857"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ODQ48710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1504" mass="170138" checksum="9E52E81C28D1C7FB" modified="2017-01-18" version="1">MFQFDLNGLFPCGCHDGEGYQWTSHLSDPSPCLLNGLFLNILSVYFLIHSLQVIHKLRKQKNINSKPNYIFASKLLLIVCQIFMQLAIVILTTKKSQNAYPPYSVFSDSVWWSTILVFISLLVSLVFSYIEFFKTYVGQTPLIIFWFFSTAIGFLRVVNSSLRHYPSTDAFLFSAFAVNSLLIFILEVSFAPKLNLETTEGYSIYDYSNIFSKLTFTWMTPLMRKGYQKFLLQSDLPPLPNSLKSKLLSSVLDKNWIFESNNSDNPSLFRALVKSFGGPFFVGAIFKFIQDCIAFTQPQLLKRLIIFVHDYHDDPSIPLTRGFMIVMSMFSLSVIQTASLHQYFERVYDTSIKVKSSMTALVYQKSLNLSIEAKKAKTTGDIVNLMSVDTQRLQDVCQNLQLIWSGPFQITLCLISLYNLLGNAMWVGVGILVITVPLNTRLFRFQKKLQKDQMKVKDERTGLISEILNNIKSLKLYAWETPYKEKLTYVRNEKELKNLKKIGIFQAGNQFIFNSTPFMVSCSTFAMFIILYKGTPLTTDLVFTALALFNLLGFPLMALPLTIGNIIESQVALSRLTEFLCSDEIEDDVINRYPAAKSVGDVSVKVEGADFLWSREPYKTALVNINYTAYKGQLSCVLGKVGSGKSALIQALLGSLHRTNGIASIAGSIAYVPQTAWIMNGTIKENILFGCKFDPDFYQKTIHACALSQDLEILPDGDETQVGEKGISMSGGQKARLSLARAVYARADVYLLDDVLSAVDEHVGKHISNNVLGPNGLLKNKCRILATNSMAVFKFSNHLTMMADGRIVEEGTYSDLLLSKKKTSLLYKLIKEYGRKDSTHSENKTEEQVSEPVSELSSAMAMSTTPSTDEVKDVKGDFRKAELEDFRIDIQNQEKVQENAGRSETHEQGKVDWNVYITYAKACGVGHVLLLIVFIVTAMALSVTSNVWLKHWSEINTAVGANPEPWKYLSIYFLFCISAVFCTMCQSLVQWLLCSISGSKYLHEIMLNSVLRAPMQFFETTPIGRILNRFSNDIYKIDETLFRVFSMFFSNSIRVLFTILIIIYSTWQFIFFVVPMFFIYRYYQKYYLYTSRELKRLESVSKSPIYAHFQETLTGVSVIKAYDQAQRFSFLNQYKMDVNMSAYHPSVTANRWLAVRLEFLGSFIILGSSGLLILTLRTGKVTPGLVGLSVSYALQVTQALNWIVRMTVDIESNIVSVERVMEYAALPHEAPEVIDDKRPSTNWPFNGEIQFKNYSTRYRSDLDLVLRDINLSVKKKEKIGIVGRTGAGKSSLTLAIFRIIEAVGGHIEIDDVNTSMIGLYDLRSKLSIIPQDSQIFRGTIRSNLDPVDQYNDDDLWEALSLSHLKEHVQRMYEEYENKEEIGFNGLLVPISEGGSNLSAGQRQLMCLARALTKKNCKVLILDEATANVDVDTDSIVQQTIRSAFEDRTILTIAHRLNTIIDSDRIIVLEQGRVKEFDTPANLLKNTDSIFFSLCEEGGLVSRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRL9</accession>
    <name>A0A1E3NRL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_70348</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019845.1">
        <property type="nucleotide sequence ID" value="XM_019163617.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NRL9"/>
    <dbReference type="GeneID" id="30180304"/>
    <dbReference type="OrthoDB" id="423559at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF16">
        <property type="entry name" value="ATP-DEPENDENT HELICASE ULS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="990"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="1337"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="1455"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="301"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="492"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1629" mass="185474" checksum="DDDF1AC0E1190103" modified="2017-01-18" version="1">MTGCLWMFTKQVPTALIIQAFRAKMATTVLPITYDSTSKKVSLEDTVPLSSSKDLQLEISQVNTLYTDFIKANSEFPPPPSKEAFTKNLSMMVKKMHESATTLMRQRQFADAAKKFDIALGLASARSKFEPFQPTMSELIICLMGRCDAYTNANMFVEALEDAEVLVLLGSQIPDNHLRRGICNLNLGEFLSAKSDFERGLAFNPKHPILLKLLDICLKIIDEENDEAKNYSPTLAFKKQQDNNLRNHLSNNSPQSPTPNPIQTNSKRNGVAHDSLNFKIDSLSFSHDPNIPKKDLQLQSHDNQFQPPHSQSSEHAPERAASNDNTGVNPDTSSDRLQTKTLIPPVADLQNLKSSKTTEPILHDHVQPRNRYPSPTQTSTHTLNSGVGRLNSILDSTFRKKVAIPTASKFLLSKSSTPELKDGKVAYDNIPNTVSMASIPRVSKAESQSSQNLGFIDLTSDDGSDDDINFTNVANMRSSTDLTRPAVFNPQEQKPFKFDSNPHNLASSLKSEPLSMKPSQENTEVTKPPFNKFAYNDSDDKSETDEEDDIIITGVGHNPTNAFDDDDDVIPLETKNSYFTQTVKRERQVETEEEMARKRQEILRNKMNMIQDNVKKEEIFQNELAERANIQKEKAERDRINKLLEKYPPIKALPLKYQIEFPTETNFVSEAMPILPDSIQAFEITQDGVTHLPPELRVTKFRDENLNAMHSVIHRLNKFSDLHSRMDAFLKKAAEMRNQVQEKAVKNSGIKKADIRYNQILRFLDNKIKTLSKYYGEVGSGIFKLKSYKMKIIERSRLLEELRKAYIFNGFTQSDITHIGTYMKVLPELNLVINTITEMYKLTGSSPAETLKTVREFSLTSQPIPPIQIPAHSDAIAPRIDNPLIPNNMQVIDVDNDEEYTNGYDNIFTQMDQYVNGRNPSYMPFSNVYDDNMNHLNPYRTNLNDSDGIRNLMESIKITEFKEEGLANTPKELCISLLKHQRIGVSWMLKIEEGSNKGGILADDMGLGKTIQAIALILANPSQREDVKTNLIIGPVSLLHQWQQEIGMKIKNENKLSTYLFHSTNKLSNFEELSQYDIVLISYQTLGSEWKKHYAKELEDAKKGNAPRVRRRDYKSPFFTANAIFHRIILDEAQFIKNKNTIASKAVASLSSTYRWCLSGTPIQNRIDELYPLIRFLGIKPYNDWTKFNQQIALSVKSRSSSANRKIHALLSAILLRRTKDSEIDGKPILTLPEKHIIEEKVEMEEKEKEFYSSLEHSSKKTADKLLRSQNKTYSSILTLLLRMRQAADHQYLVRLGDDGDRVSKLDRFKKGFEAVKDYPNNVIRRIEDERENGFTCLHCTEELAEGQVLLLSKCGHPLCYDCHTEYFEQHSEASWEGDVSAKCISCGVTNLSSMSVDLRVYESYREGLTWQEIRKRYQLDSRASDKNWRLATIKKFIEEDGKLMISAKANKTIEIAENILNNKPGEKVIVFSQFVGFFDILQITLRERNIDFLQYDGSMDMTTKNDCISAFYKDPSKRVLLLSLKAGNVGLTLTCANHVIIVEPFWNPYVEKQAQDRVHRISQTREVYVHRLLIAGTIEDRIMELQKEKEELVESALDPTARNKIGKLSRRELGFLFGLNGLAELEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSA5</accession>
    <name>A0A1E3NSA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Golgi apparatus membrane protein TVP23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_18818</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Golgi apparatus membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TVP23 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019576.1">
        <property type="nucleotide sequence ID" value="XM_019162087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSA5"/>
    <dbReference type="STRING" id="763406.A0A1E3NSA5"/>
    <dbReference type="GeneID" id="30178774"/>
    <dbReference type="OrthoDB" id="2151161at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="179" mass="20174" checksum="4150745996830CAC" modified="2017-01-18" version="1" fragment="single">PQTLYQKLSESSHPVALLSFLLLRLTPIAIYLFGMLFTSNYILLFITVILLLAADFWNVKNISGRLLVGLRWWNESNELGQSIWVFENADPARYINPIDSKVFWALMYVVPAVWTVLAVFAVLKLELLSLILVIVSISLSLTNTMAYTKCDKFGKANNIANEMLSSVGGSLFSRLNFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSA9</accession>
    <name>A0A1E3NSA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020053.1">
        <property type="nucleotide sequence ID" value="XM_019160380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSA9"/>
    <dbReference type="STRING" id="763406.A0A1E3NSA9"/>
    <dbReference type="GeneID" id="30177067"/>
    <dbReference type="OrthoDB" id="629492at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="655" mass="75492" checksum="A11CB5CEB90E62CF" modified="2017-01-18" version="1">MDAEERNLKKGIQLFKRQDYHGSIDVFVAVVHAYQDEFNKNRMRLERNAKRKTGRKGDEKVCYNKNYVNALDFLSAAYLKVDNLEKALRYARVMVSLQPLSCKGYLRLSKIHLLLRNDRRAYDILWKGYLKIREAKNDPAKKFVVNETLYRQLKDELQKSKNSLQSGTESVEEGMPGSQHVEAKDNDPLKVLPFELLCNIFAQFPLPFNLRCLCVSSHWNETLTSTARIFESFHLKKNIRKQELTEFFNFLGKIYRHNNGRVFLKFVNIEPHESVEKSILQLFFSKKLIIESLKINLNSTNLFGLNPIIKNGKVTFSHTKSLNLRVPLFVNETVSLLDILGRCENLEKLVLIIPKFDSRSKLVQQNAVRFAKLHLLSVSVEKEAANSIFNDTVMNNFLGQNVFPKLRHLTVSRVKLSQKALKKILNPHIETIELDSVPGISIASLLDAMLGNTEPLSTLRELKIVETETTMDSSHRDWRNELLNSKLLSNLSVLMLRNSCITPKMLEDILLASLCQIKSLHLVLNRYIVFQNRLTNPSSTQTPYEYANISNLIAKVPHIEELSIVGCPGFNQLTLSELSKEAMTNHLFDKLFYLNLSMNKVEDNSLIQLFRKPYNLKLDKLVIQYCEVSPGTVKYLLDNKMCRSIDYKMSDRVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSB5</accession>
    <name>A0A1E3NSB5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_90945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020160.1">
        <property type="nucleotide sequence ID" value="XM_019165065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSB5"/>
    <dbReference type="GeneID" id="30181752"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="237" mass="26919" checksum="3D25E0BE3329D59B" modified="2017-01-18" version="1">MCRYAVLVYVDRARRSSRYVLCLQAVWPASLLYHIERKRGKKRTTRENSMLRKRPLFVAGRDGYHLICKGVCECASSDWLAVSGRRGARRKHRGRPHTCAERGLDGALRGLTGPVLRDCVSGKTSASWAQWACEGVAGAREGEGGRARGRETEEGVKNRRRFSSCSDRKKAEDGDRGSRGVITSQLKTQIRMYRHHQEALRRQEILVSDKRRACQAQSEECRLQNANKEWRTITNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSK3</accession>
    <name>A0A1E3NSK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleolar complex protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_30584</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020228.1">
        <property type="nucleotide sequence ID" value="XM_019162641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSK3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSK3"/>
    <dbReference type="GeneID" id="30179328"/>
    <dbReference type="OrthoDB" id="441771at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="283"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="283"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ49115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="862" mass="98343" checksum="B66479085E61D8AD" modified="2017-01-18" version="1">MAGGTQLKKLKESLKNAGLTGQSNLGKKNKGKNRNGAQDKLRKDERQKALNEIREQFNPFDVKVTRNKRADAIDKKLTVGKPGINKQAGEEARRSEYESRLERRGKVGGIIDRRFGEGNSKLTPEEKMLERFTKERLAKANKSSMYNLEDGDNIDDEDNQDFGELTHLGQSLSGKTTTTGISIDNDDFFSRKRNGDTSSHEEETNGEPVRKKTKAEVMKEVIAKSKKYKHERQRAHLENMEKVDELDDAFDNVMDEISVVNRGKQDDAGKSKFDIDYEMKVTEAKLDKRAKPSDRTKTEDEIKQEIEEKKAENEKKRLQRMEGEEDDEDENRVSGAVADDLGDDFWAESGDEETGFSVGSPVDTVSSDENTDVESQSDDNESRKPKSSDSKENIIKIGNKTIVVKPKILKSKLTCPQNLKDFKSQIIEDDFENVVSTIKKVYEIYQPKLAEGNKEKLGVFTTVLFEYLLELSNKTIGAENHEYVRLMDFLARTICNLTEKYQELFLEAFRKHITDAHERLLVQDIAKFPLQSDAILLTLVGRTFSTSDKFHLVVIPALLVAGEALEFMSPENNKTQMFFGLYLCDLLLQYERISDRVIPEIISFLQRLLLATIPCPHALEDWQKVLICSVAPVPSDYTCTSPCDIPSETEKLSISKMWALSLSSESEDEDTFIKLIILKVVRTLDLVISRMIKNTSAAPELTSSFIPLMRHLIKHTSTPNTTIINVANKLTNIHRISTQERRPLKLQKHRAVGIAQITPRFNENFNPDRKSSRSVNMDPLDPVAVREEISKLKHQVKEERKQALKELRRDAKFEARAQIATKKKEYDDYHSKMAKIYNSIQTEEGTAKNEYEKEKKARKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSL1</accession>
    <name>A0A1E3NSL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_70695</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020238.1">
        <property type="nucleotide sequence ID" value="XM_019163707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSL1"/>
    <dbReference type="STRING" id="763406.A0A1E3NSL1"/>
    <dbReference type="GeneID" id="30180394"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000726">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="280"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="469"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ49125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="906" mass="102892" checksum="1C34991B815CA48B" modified="2017-01-18" version="1">MSGNKGRFGKRVRDSETWKDDLDLPSDLESVHTSKKRIDKKVSKTTDGNEEKNIDKTDAVLDEEQMLFGNKISLESNGNNGAALSPQQPIEALPHTLELPKREVDILRLEVTHFQERVARNGLTSLSAEDKSEFQSRKELLQKLQQNNHSDNYLLPDDYITEEGKIDLAKKAKALKMNMRAERKQRENEWYENQINRAQQVNQLSKLKKETTDETEIKYEYVFDKSHMIDYVADVPEDEMKELENKIELEELIEQEEKRVYSIEETKKSLPVFQFRDELLNAVKKYQVLIVVGETGSGKTTQLPQYLYEAGYGKHGKIIGCTQPRRVAAVSVAARVADEVGTRVGDKVGYSIRFDERSSENTVIKYMTDGMLVREFLTDHDLAKYSVMIIDEAHERTLQTDILLGLFKDLLKKRPDLKLIISSATINAKKFSDYFDSAPIFNVPGRRYPVEIFYTQQPEANYLSAAIITVFQIHISQNSAGDILVFLTGQDEIETMAESLAETCKKLEGQIKEMVICPIYANLPPEQQKKIFEPTPKGARKVVISTNIAETSLTIDGIVYVIDCGFVKENIYNATTGMESLEVLPCSKASADQRAGRAGRVGPGKCFRLYTKWVYLNDLPRNPTPEILRTDLTGVVLLMMTLGITDLVHFDFMDKPSTKTLVKALELLYALGALNERGELTNIGIKMAMFPTKPMLSKSLLASSELKCCDEVLSIVSMLEDTGSLFVNSKEKKEMAKNAHASFHSKIGGDHLTLLQIWNRFVESGFSPQWCHDNFLQYKALQKARNVKKQLQRICRRLGLLSDDRQEPERDPNTTALHVMRAITAGYFPQSARLSPRGDTYTTLSRKQPVLIHPSSTLHGAVPPVKLIVYHELVMTTKEFMRHVMPVQGKWLAEYGRNYFDKKDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSP0</accession>
    <name>A0A1E3NSP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nuclear control of ATPase protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_70691</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020232.1">
        <property type="nucleotide sequence ID" value="XM_019163705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSP0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSP0"/>
    <dbReference type="GeneID" id="30180392"/>
    <dbReference type="OrthoDB" id="413313at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013946">
        <property type="entry name" value="NCA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234:SF1">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08637">
        <property type="entry name" value="NCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ49119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="692" mass="79502" checksum="0F00AF21743084AE" modified="2017-01-18" version="1">MSTLTLQTFLGNSNIQELQDFATVLLSRMEKEYPGDSVETEKVKSLFIDVYQIIKPGLKSSPGEGSGTTAPKISQGEDRFKSLSQAEEQDFTLSATGVDVLNCAILQIRDNLWLESEDSNIRMLSEIMVLNILVTLMLEISKTLITSTLNTSNTINYYDCVLSKKYSVLLYFLQTLPQNTFAFLMEVKKVKLVSPSSTIVEIPSWIPNFFRDIYAATLKYFRLAYTMIDRSIEDFFRSPTTFLMERQQNSRSLFVKFWNSTFQLPYYYSKYEIERRRKNLVNLQKTNISKLGYLLTNSPHYDLKNDNLSTDFSTINSFLESFLSSDKFTQSSLPKEKQLNIDNLYSAFSTGSYELKHKLDRVESENAMPSYFTRNWPIIIPAGLFIGSYVPSALRNTRLLITDPQVRSESYEYFKQLVDYCIDTTVSFWKHWVVNPINNILKTIRHDDNSEIALMSQKSLASDLDSLERMAIDYIVDSSPTGGNMSLSELDQIKNAIKSGDMTLVMNNYERDLKTPIKSIVAGDMIRNILIQIQKTKVDGSLALNGVDQILKSQELVFGFVAASPSLFIVWMLKNSFMSWYNGESKSSYKNVQTREVKTRVNASLGKIEKLVDYMVSQKRDNSVSDDVDYYKNGLVFIEVRSLKRLSKKLLPLYVYYQFKEDVEELVGTSSDVEYKLLTIQRIWNVYAGYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT13</accession>
    <name>A0A1E3NT13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS72/YL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020354.1">
        <property type="nucleotide sequence ID" value="XM_019160492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT13"/>
    <dbReference type="STRING" id="763406.A0A1E3NT13"/>
    <dbReference type="GeneID" id="30177179"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046757">
        <property type="entry name" value="YL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 72 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275">
        <property type="entry name" value="YL-1 PROTEIN TRANSCRIPTION FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05764">
        <property type="entry name" value="YL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="3">
        <location>
            <begin position="633"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="462"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ49241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="708" mass="80566" checksum="DC2D6ED26C415181" modified="2017-01-18" version="1">MSESESSESEPEFESIIATRARRSNAGSRLRQLLDLEETNNGPVSINDDDENVNLLFMEDEDDGEFQDSEDEEQKDGDEDEDEDEEEEVDVEAELNKTPREKKKTTNDSGNTSEEEKDLPNVDADEMLSDSDISASDDDESEGEKELQKQEQLNRRRKRKASLPAVLQKKKPKVASSKDSTKAASKLATKSHRSNIFDSVPIAPYERRHSARRTTLQNSMATHEKMEKEYEKRQTQAPVVSKEYVEKTLEERLEEAKITEKENTLSLTRFYEQEIQKKKKQRDLANSRKFKMRNFIRFWSTGIFITPLDEVRLIEEEERRLKEEEENKNRRKLQYLKRKQARLAKTGSSSPIKTNTKEQSEIDLMANKIKDDEDKKVTSAIELKQETEVNPVKKEEITTAESVDENTPTNAVQFSEVSIPLTDEQKISNDAESNPVDTTEKKFKEVELETTDIPLKELSLGGSFDESMKETSTLEGVKREGNGKTDNEDENTQEDNVNESEVICDTPKLQFEGPAQHVARNYVIFEEFDHDVTSDGVKRYLFGQQAAIKGTHHDPQYETLCVIKQDESSNIDLHKIQAMREESFKSLIQLPKFGEKITAIDDGNGQLEEDENEVVQIHTPAPVGIHLPNGQKKICPTSGLPAMYYDPTNGVPYATVDAFRVLKSIVEGDYEWLQLDHGGINSRFKGGIGCYISKKNQRHAAGVPEGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT27</accession>
    <name>A0A1E3NT27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020420.1">
        <property type="nucleotide sequence ID" value="XM_019160525.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NT27"/>
    <dbReference type="GeneID" id="30177212"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF405">
        <property type="entry name" value="ARF GUANINE NUCLEOTIDE EXCHANGE FACTOR SYT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="450"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="721"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1261"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="733"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ49307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1489" mass="167360" checksum="50376B1EF2F61A92" modified="2017-01-18" version="1">MTLSPPLKQNEHGLLMPRQEPQNTECRSPTQLKVMITEASPSSIGTGSARSDSVAERDAVELSNTPNHAADQGHPDNREEEVKCRIEEHMSPSHDRHQTELSHTPQSKISLKFKHSVSTNWLPSMRKKYPATGENPVLVETEKEYDSYLLEKAQRNDNTLGKQTNSKGFFPKKSAKRISLGHTSLIPKRFSLNHQAHSQVKVDGTLGAAATFSSQSSGKKSNAKSPTIKDSKNLASLPPSISPLPLSPNTSAANNTTPMMPSSPTSIYQAAPYRQALDRNTSFSSSTPSEKSTIGKSLNAFKRTTKSILFLESNSNGSASQSSGANNCNYNYNYNYNLNIVDSPLLNRGNSEELSVIRRSISYTEAERKQAIFYADNSREITRRNIGYDSPIKACTYDDSDPNCECDETSVSDNDSNSIEGNDSDDIEPSSIRKHKNKPMPKDKRQTSKTLAQYRQRSKTIDNSNNPLAELANNTNTGLLSSLTNFVKINRSSVSTSSSAVKLNKISQQLPKLDHLEVIEAETPAEFLSRLLCIYPLTFVCEVLSSEDNEILKQTLQEYLKTYYDFSNVPLDIALRTFLMLNHLPKETQQIDRFIYQFAKYYHSQHPELGFDQDTVYILTYSLIMVHTDKFNPNNKRKMTRFEFVQNVLSALENNYNSFSNSNELTNLILKELLGYFYDNITYCPLIKISQEQSVFALEALTLKAFPFPYPNITFLNSSTTGKNSDHDYRKASVSSSSMSIPSSSTSTHRSNSQQVLQAQQPIRKKSSSFLWSSTSTMDPYDYIVKNDTGVFNELKFLANEKDLNLTCQNPFILPSTTEVDNALDEKSPTSDMIGQQLNEYGSLMDREIDPEFLCHIWKSLTDSRIGFTLKVPKEKGSYLVSSKTEAIPLNEADGKEFYLTRVIKVGLIDRQENSASQNDPTSTPVPTQELAELSINNDIKNSHAKSQLINKYKNTVWKRYFCILTVVGMFFFKDVSSFRMKFCGRNDTDGTKMVIIEESSRIKPQFESFSTLANGNSNTVSNGSSNSDSSGFISFPHFRSEKSDKFEKIEKEKSESVLPSFVMSNGSFAVRKLENIEYDEIINDMNTSTVVTTPQSTSESSAFNSVPSVLSVTTGVSPKLSAQLPQIRNFQSSVPEVNVHQISEGVMDPALDTQPKKKNIVNPETWKYTFFIYGRMSRNIFMVSSLTELKSWIHSINVMSVLNGVKVDHKPLDYHIIPKIYSSKCNEAEGEPKYYEVTPQCNISIEQRFAHRASDDLLSTDERDEGSESFESGKNDVQVDINNDRRRNRSSTTFLSKLAPSMPALGLGLNNGSSKIDSEKKTKLQDVDKFSDNFSSDSDSNQDYATASLKPSFICEDNNSESYLKEGAQTNSNESGAYKHEITQGDLDFKEEDTSEFNTSEVPSKSSSANSKNFHQYLLKHLYSVKRLTLTMPLQKKTREDMLSTAKILGVKLEWLWYEKCRSQTISVISKRIMVLNENSSDRVLFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT46</accession>
    <name>A0A1E3NT46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14262</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019846.1">
        <property type="nucleotide sequence ID" value="XM_019160299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT46"/>
    <dbReference type="STRING" id="763406.A0A1E3NT46"/>
    <dbReference type="GeneID" id="30176986"/>
    <dbReference type="OrthoDB" id="76567at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017340">
        <property type="entry name" value="U1_snRNP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148:SF1">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="4"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="124"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="247"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ48733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="282" mass="31734" checksum="741BF80B41AC1855" modified="2017-01-18" version="1">MGKYFCDYCQSYLTHDSLSVRKNHLHGKHHLRLYNEYYEKVANEHPEYDRKNTDASVGNPLLSEMYKGIPGLTNKYADQHQRDSGAKPAFKLYVPSTSVGLPNPPPNVLFVNYSNYVQKSRVTKPYYSNGNSNGYGHGHDRGHGFGGFNRGGNRGFRPRDREASPRTTAGTSGRGSTDHRQTHRRPAPAAASGTDLGHRNPSSSAPVRTGPHNGYGYGYGYDSKDRPSRPAGGQGYAQARHGQGDVSQQQQPPQRNRQSYQQRYKQGYQRYPGQQDRSRDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTA3</accession>
    <name>A0A1E3NTA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="11">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_14761</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11 14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11 14">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020403.1">
        <property type="nucleotide sequence ID" value="XM_019160518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTA3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTA3"/>
    <dbReference type="GeneID" id="30177205"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF21">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="34"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="119"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="345"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ49290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="441" mass="48800" checksum="F85F26820D88FCD7" modified="2017-01-18" version="1">MYSAAARAQGRLAAINRHFSTSSASLGKIRVKNPIVELDGDEMTRIIWSDIKKKLILPYLDVDLKYYDLGIESRDATDDKITVDAANAIRKYGVGVKCATITPDEARVKEFNLKKMWLSPNGTIRNILGGTVFREPIVIGSGPDKQPGDIEIPRLVPGWKKPIIIGRHAHADQYKATDFVVPEAGSLEITFTPKNPSGEKISRKVYDYQGPGVALAMYNTEDSIRGFAHSSFKMALSRKAPLFLSTKNTILKKYDGKFKDIFQEIYESSYKADFEKAGITYEHRLIDDMVAQMLKSQGGFVIALKNYDGDVESDIVAQGFGSLGLMTSVLITPDGKAFESEAAHGTVTRHYRRYQAGEKTSTNSIASIFAWTRGIAQRGKLDNTPDVVEFANTLEKCVLDTVSKDGIMTKDLALLVQRKDYADTEGFLDAVASRLANTLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTB4</accession>
    <name>A0A1E3NTB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial GTP/GDP carrier protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70890</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020454.1">
        <property type="nucleotide sequence ID" value="XM_019163763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTB4"/>
    <dbReference type="GeneID" id="30180450"/>
    <dbReference type="OrthoDB" id="409947at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001409">
        <property type="term" value="F:guanine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000010">
        <property type="entry name" value="Probable YHM1 (Mitochondrial carrier)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053042">
        <property type="entry name" value="Mito_GTP/GDP_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974">
        <property type="entry name" value="MITOCHONDRIAL GTP/GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974:SF1">
        <property type="entry name" value="MITOCHONDRIAL GTP_GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="207"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="32261" checksum="5DB96EFD24E404B9" modified="2017-01-18" version="1">MSTVDKKQSGLARILGPAVAGVCEIGVFHPVDTISKRLMSNTTRVNNLEKLNSVIFKDKASAPAGKRFLSLFPGLGYAACYKILQRIYKYGGQPFANEFLTSNFKKSYDDLFGPKTGKALLSATAGSLIGIGEVVLLPLDVLKIKRQTNPEAFKGRGFFKILKDEGFSLYNGAGWTAARNAPGSFALFGGSAFAKEFIFGLTDYSSASWYQNFVASICGASASLIVSAPLDVIKTRIQNKNFDANESGFTILKNMLKNEGPTAFFKGLIPKLLTTGPKLVFSFALAQSLIPAFDRMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTH4</accession>
    <name>A0A1E3NTH4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_101033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020524.1">
        <property type="nucleotide sequence ID" value="XM_019159629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTH4"/>
    <dbReference type="GeneID" id="30176316"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="186" mass="20729" checksum="438BBA4159A989FC" modified="2017-01-18" version="1">MGCRRNRRMRSAPLLPNGFRLCCGLRRRLHRSRRCARSSGERGRDGKKHGSGRTQGQAIAVSRQRAPPRSPARRSLYLVSAAPHRLSRLIALRFTPMRKASAGLEPALADTRTRSLSAACFIYFDRCTRSKSCSHGLCGRLQRLQQASAGFSRLQEASEDPNSRGLPVPTRSVRGTLGLRGVHRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTI1</accession>
    <name>A0A1E3NTI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial acidic protein MAM33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_9892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020534.1">
        <property type="nucleotide sequence ID" value="XM_019165139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTI1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTI1"/>
    <dbReference type="GeneID" id="30181826"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902775">
        <property type="term" value="P:mitochondrial large ribosomal subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29732" checksum="89FBA020AB3111BA" modified="2017-01-18" version="1">MFSRPIVHTARSSLRSSLLGSQHVAALRRVAAGAAPRAAAVNAGNRASFFSTSVSTKNEAKTQLHEVLSQELKFEESDAFGLDESFKTYLNENNIEIVKTDGKVLAELVKKTPTENIHIYFDILRISQTSYQLKQMQEQVENSEYLEDEIADIAFADVNVVIVKDKKATGFDLSLSLIDSSFSVSAITNFEDAKLALDESPEASAVRDLKYSGPEFNNLAEELQEAINQYLNSRGIDNALADFILAYSGVKENNEYLDWLDSLKKFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTM0</accession>
    <name>A0A1E3NTM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_18899</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020527.1">
        <property type="nucleotide sequence ID" value="XM_019162093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTM0"/>
    <dbReference type="STRING" id="763406.A0A1E3NTM0"/>
    <dbReference type="GeneID" id="30178780"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="51" mass="6198" checksum="20E24CFC7FD63739" modified="2017-01-18" version="1" fragment="single">HECPLCHMKFQRPEHVKRHMLSHSSEKPFECPESGCNKRFNRNDNLKQHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTN5</accession>
    <name>A0A1E3NTN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sm protein G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_29967</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020141.1">
        <property type="nucleotide sequence ID" value="XM_019162614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTN5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTN5"/>
    <dbReference type="GeneID" id="30179301"/>
    <dbReference type="OrthoDB" id="2146at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF2">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="4">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ49028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="74" mass="8280" checksum="A2ABF296197BAC57" modified="2017-01-18" version="1">MVAVPELKQYLNNQIKVELNGNRVVQGKLAGYDFFLNLTMSDCVEVKGPKTNPEYIQLGQSVIRGNSIINIELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTZ1</accession>
    <name>A0A1E3NTZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_14989</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020667.1">
        <property type="nucleotide sequence ID" value="XM_019160625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTZ1"/>
    <dbReference type="STRING" id="763406.A0A1E3NTZ1"/>
    <dbReference type="GeneID" id="30177312"/>
    <dbReference type="OrthoDB" id="10264038at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11368">
        <property type="entry name" value="RNase_PH_RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033100">
        <property type="entry name" value="Rrp45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF14">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="36"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="164"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="326"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="283"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ49554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="415" mass="44637" checksum="7CC0B3321D08B5C0" modified="2017-01-18" version="1">MPNTDNTLPIPNILSLHSLLSGNPPLRPSLRPLTEPRPLDISIASSSEYGHVEVKLGKTYVSCKVSAQITSPYEDRPFEGTFAIGTEITPMCSPAFTSGGNSDFSTAAPAIVPAPAISVSANNVENSEGKDSTSSSVLASSNNNTNSDSAVTMGGGAVMGIAADQTATEVLLSRLIEKAVRRSNSLDLESLCIIAGQKCWAVRADIRFLNYDGNLVDAASVAVIAALLHFRKPDVEVVGSGEGSELVMYDEKQREMVPLSVLHIPICITFQFFSTESVERNIKGENSGGLHSRGKNYVKEESDDEDNNRMEVDNDDDGPRGSNEIIIVDATAEEELLSQGSMTVTLNSNKELCQISKSGGLNVDAMHILECCQVASGIVDKWTKEIKKVLATDSEKRRDLRAERVLSVVNDRSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NV92</accession>
    <name>A0A1E3NV92_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_102262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020576.1">
        <property type="nucleotide sequence ID" value="XM_019159650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NV92"/>
    <dbReference type="GeneID" id="30176337"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="119" mass="12895" checksum="CC44B53AABACB90D" modified="2017-01-18" version="1">MVANRGNVWCRRVARSDGAKTRLEGFPAAIPGLSRSSGSGTCLSALCCSSDRPPQQYSPSNACLIHVCQKKTLHRLFLPSAGPSGPDGPSFLPARFAHEQSRRRFPFRTGRVPRRGVAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3ND70</accession>
    <name>A0A1E3ND70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_74908</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015183.1">
        <property type="nucleotide sequence ID" value="XM_019164906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3ND70"/>
    <dbReference type="STRING" id="763406.A0A1E3ND70"/>
    <dbReference type="GeneID" id="30181593"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF29">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="537"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="541"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="556"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ44070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="63460" checksum="A7C63BF7405C90E6" modified="2017-01-18" version="1">MFVDTVESRTSYAVPVSKDDEVIRSLGYKPEFKRDLSLWATFSVSFSVLGLIPSVASTMWYSIVYGGNAGLTWGFLIGMTGVMAVCCSMCEISSAYPTSGGLYYATAMLSPPKYKAVLSWTVGWSNYFVQLTGGPAVGWSCASIILALKTMTDPTYENKTWHTYLLTIGITFTCACIASCPTRWIARINSCTSILNMVFLFVSWIIILAANNRTNLGLPKFNDNRKAWGIDNFTDWPDGICMLMTFLAVIWTMSGFDIPFHIAEESSNAQISTPRAIFMTASIGGALCFVYQLSIAYTIVDVEGVVNSEIGQPYVSYLAQVLDRRSCLALASFSIILTFSMCFTSMIAASRVLFSYSRDNCFPMSHIWCHINTKTRTPVNAVWMNWFIGACLDLLMFGGVAINAIFSLGAIGSFISFTFPTLLRITYARNTFVPGPWNMGKWTYFCGWYAVGFVLLMIPILNFPQFRGKNLTPELMNWTCLVYYGAMFLALAWYFIYAHKIFKGPKSNITNTVIQNYEELEEQVDLVEKIADQEAEAKTTGHVSLNSVEATSSDNVSKKNSEFVKKEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDF5</accession>
    <name>A0A1E3NDF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_13902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454009">
        <property type="protein sequence ID" value="ODQ44152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015265.1">
        <property type="nucleotide sequence ID" value="XM_019160123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDF5"/>
    <dbReference type="GeneID" id="30176810"/>
    <dbReference type="OrthoDB" id="2392544at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="2">
        <location>
            <begin position="55"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="55"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="187" mass="21979" checksum="3EF00F89326CD78C" modified="2017-01-18" version="1">MYTFMIYESETKPNETESLQRSPVSFEFTDIVKAEDGLNQKKLREIKKSRVELIKRKPRNMFMIFRGLVKHLILKKIPNGSFADVSKISSMAWSKRTPDLEKYVKYLSKQEETYQNRHCLNNPSLKKDEARTRSPLNNASIVGFNCRVFKHYQVTKSRSKSKPHHSVTTRKLFPGKFRIEDIYVNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDN5</accession>
    <name>A0A1E3NDN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_18489</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015354.1">
        <property type="nucleotide sequence ID" value="XM_019162019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDN5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDN5"/>
    <dbReference type="GeneID" id="30178706"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815:SF1">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="174"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="437" mass="49268" checksum="E61CE8A2DEE9CF9F" modified="2017-01-18" version="1">MMPSISSITLDGPKDNPGDDPDGPDGETADIPLTPERVEKVKEEEANDDASESIEEVLNDDFHLPKDVGEINVKGHLHFDEEMPPDETTQNNTMHNAMMYQLHDDRDDHRDHIHHHHHHDHAEHEHEREEKEHDDRSGKRSIMFDTYSPNQDVSHYQMTIVHEDKHFHYRNTTRTFLVPYDDEKPSADALAWTFTEMAADNDIVIVLKVVSLQHVMKYGVQSHRKDCQNLFKMVCKCNEERRKLKVVVEIRIGSVEYSLARALKDFDPTFMVMGTRGIRKAKLTSFLNEDPSMTKHFLDDGWVPVIVLNPNYVHGKVAAAATAAAAATPAKNTFRNFLTSYPSVYDPLVAGQTLEMERSVSGESGTSSGAGRTSRFLRIGRSMSRSSSINRHNNSNDSPGGTLSPTTSNDLSPGRALSPRRALSPFRLFHNSRHGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDN6</accession>
    <name>A0A1E3NDN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_69833</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribonuclease III family. Mitochondrion-specific ribosomal protein mL44 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454008">
        <property type="protein sequence ID" value="ODQ44230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015343.1">
        <property type="nucleotide sequence ID" value="XM_019163513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDN6"/>
    <dbReference type="STRING" id="763406.A0A1E3NDN6"/>
    <dbReference type="GeneID" id="30180200"/>
    <dbReference type="OrthoDB" id="67027at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19873">
        <property type="entry name" value="DSRM_MRPL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044443">
        <property type="entry name" value="Ribosomal_mL44_DSRM_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044444">
        <property type="entry name" value="Ribosomal_mL44_DSRM_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF32">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22892">
        <property type="entry name" value="DSRM_MRPL44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="8">
        <location>
            <begin position="122"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="375" mass="41935" checksum="9308168EB54006C6" modified="2017-01-18" version="1">MFAAAKTTAQRGVSVSLWTRSISVSPAAPQHPPKNDTEVSRKYRFSYETAKNKYVKESDLNVPQDKFDFASYENKTNSTTVSEENAQLIPSIVALHARLQLPEEYKLSTLVKSLSCPMGEGNASNNQQMTIFGSHILSFYVSEYLLANYPRLPISILRSAIDAYIGNFSLYDVAKNSWGIDEDASSKLDKYLSNEPELFKYGRLRYLRKVSTPESNVVKYSPAQVNPYEISKSEAFANSVRSIIAGLYVHSGETATKEFITNHILSRQVDISALFEFKEPGKLLTRLLKTNNMHPPTVRLISETGRLSNSPIYVVGCFSGNDLLASGEGSSLREARVRSFVRALKAWYLYKPLDAKVPSDASFSGMFVDDGEKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDX0</accession>
    <name>A0A1E3NDX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aspartate--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_37021</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="3"/>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015409.1">
        <property type="nucleotide sequence ID" value="XM_019163048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDX0"/>
    <dbReference type="STRING" id="763406.A0A1E3NDX0"/>
    <dbReference type="GeneID" id="30179735"/>
    <dbReference type="OrthoDB" id="372395at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000013">
        <property type="entry name" value="Aspartate--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000132">
        <property type="entry name" value="Aspartyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="245"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="552" mass="62471" checksum="FA52AB64EA6E24F0" modified="2017-01-18" version="1">MSEEVTKKLDEVSLSAEQPILGPDGQPLSKKALKKLEKEKEKERKKAEKAAQLAEEQAAREAAALANDTAKDNYGKQPLMQSKAEDVTGVKRTKISTLSAANDGEEIVFRSRVHNARQQSANMCFFAFRQQDALVQGLLKSNGDSISKQMVKWAAGLSFESIVLVHGIVRKVEEPVKSATVQDVEIHITKIYLISAAPSQLPLLIEDASRSDAEAEASGLPIVNLDTRLDARVIDLRTNTNHCIFELQSGICALFREYLSNLNFREIHSPKLIGSPSEGGANVFEVTYFKTKAYLAQSPQFYKQQLIAADFERVFEIAPVFRAENSNTHRHLTEFVGLDMEMAIEEHYHEAVDVLADMFIFIFNELKKRYANEIATIRKQFPVEEFKVPEKMVKIHYKEAVKMLNDNGKDQGAFEDLSTENEKFLGKLVRDKYDTDFFILDKFPLAVRPFYTMPDPEDPNYSNSYDFFMRGEEILSGAQRIHDPELLRERMNFHGVDPDGPGTVDYVNAFTYGCPPHAGGGIGMERVLMFFLDLKNIRRASLFPRDPKRLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDX5</accession>
    <name>A0A1E3NDX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_74582</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the bZIP family. GCN4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015444.1">
        <property type="nucleotide sequence ID" value="XM_019164808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDX5"/>
    <dbReference type="STRING" id="763406.A0A1E3NDX5"/>
    <dbReference type="GeneID" id="30181495"/>
    <dbReference type="OrthoDB" id="5419235at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12193">
        <property type="entry name" value="bZIP_GCN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001491">
        <property type="entry name" value="Cross-pathway control protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF35">
        <property type="entry name" value="TRANSCRIPTION FACTOR JRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="10">
        <location>
            <begin position="210"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="120"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="260" mass="28737" checksum="4F2AA22C34683E6D" modified="2017-01-18" version="1">MLLGENMFSTFTSLDAGSDNVANSGDFMLNEVPKQESNDLLLDSLDFNIMNENLIDGFQNTTDSAPEDKMTPFVVDTKVFESVFKNSEDPLLDDIDSIDIADGDLKEMFEMVDSEINNDVVIKQEEQDDSEVRSKSTSALMSSRSSSASASASPMSSTSCKRSFSTACLEDSEAESLKKDSLGCTPYTRKQRNNPLPPVVPKGGDVASMKRARNTEAARRSRARKMERMSQLEDKCNDLLKENNDLKAQVQALKQLLGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NDZ8</accession>
    <name>A0A1E3NDZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SBE2/SBE22 middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74607</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015474.1">
        <property type="nucleotide sequence ID" value="XM_019164817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NDZ8"/>
    <dbReference type="GeneID" id="30181504"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053949">
        <property type="entry name" value="SBE2/SBE22_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13270">
        <property type="entry name" value="PROTEIN C20ORF116-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13270:SF14">
        <property type="entry name" value="SEX DETERMINATION AND DOSAGE COMPENSATION PROTEIN SDC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22874">
        <property type="entry name" value="SBE2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBE2/SBE22 middle" evidence="3">
        <location>
            <begin position="375"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="775" mass="86537" checksum="30FA647A46BF91D1" modified="2017-01-18" version="1">MPITEKKRVASLSRGFGISVIDTGDSKQMSTGKNRVGSRGQQQQLQHSPELPRVQTLVPGATSLDDMDGDVSIVSDSTLSEIVIDGGSNASTNANTSGGDKNDLRIDVSLSSSTLRRSSFNHQKQQQQQQQQQQQQQQQQQQQQQQQQKQQHHNNPQQIIRASAMSQRFRSTPSLHSPSMPSSSVTSPTLLQSRSNTSSPYLAQSRRSHHNLHSYTTTRLPSRRSCQQLTKKQKDKLYDDDNDTFDDTDNDILMYNVPIASTASLRLFQNGNALESSEALRKAWNESESNLIIQPSPLPGKLSDSMTAFTSPSEAIPPPTSSSTASTPTPTLSSMTAPPSATTTSAAITAITAASTSDLPRTPTTGPGMKFESPSLMKNPSYSTLSPTAQQISTFYEFSSHTHAEEELRRRKQQSTPTTAAPGLTSALDDLSMASAEKLSKLSVTRPSWIPPKDQTELHRHEKEFKKMIERTSKEALKQSRQQIKLQHERTIANARLNYLSEKNSLTSANCNEVRKYILITEVSRSTKFKLFRKMVSYKLGEGVLLSPPYQKQASSDDSFDENLEKLDIHTLLESVPTKPTHDEMSSLQTILQPLTRPTSLKDVDVSKLPKIPALPIQTLLPRFARIALTLLRSGYSTPQVRDIIYWLHAAIFTVKFKETFAKLLSKQYVLKLFKEFKDDYSILSVPTGLDLILDLDDFYVTKCIESLIVFWALGGGRGVRVFVAIVICVIREYHFGWNNLQVIFHSKAHVYLGDGEESQEKFFGRALSYYTLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE32</accession>
    <name>A0A1E3NE32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-binding protein RAP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_74639</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of telomere length, clustering and has a specific role in telomere position effect (TPE).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
            <location evidence="5">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015510.1">
        <property type="nucleotide sequence ID" value="XM_019164829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE32"/>
    <dbReference type="STRING" id="763406.A0A1E3NE32"/>
    <dbReference type="GeneID" id="30181516"/>
    <dbReference type="OrthoDB" id="435460at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070187">
        <property type="term" value="C:shelterin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031848">
        <property type="term" value="P:protection from non-homologous end joining at telomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11655">
        <property type="entry name" value="rap1_myb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015280">
        <property type="entry name" value="Rap1_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039595">
        <property type="entry name" value="TE2IP/Rap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466">
        <property type="entry name" value="TELOMERE REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466:SF6">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09197">
        <property type="entry name" value="Rap1-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="BRCT" evidence="8">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Rap1 DNA-binding" evidence="7">
        <location>
            <begin position="322"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ44397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="631" mass="71686" checksum="91EF49B56EAA218C" modified="2017-01-18" version="1">MSGKSDIFVDAAMKPYNFYLCPPLNRSERLVDMVEDNGGRVVHDFLQNCIVISSPDYVVPEDLKTAHIYSYEVIQDSANRGAQQEFANYLIHVPTKNRESVPHQYSERKDQPGAGGGDDLSGIGGTGDDYNFDVPGHGHAGAGEVSGNGNGNGNGNGADINDGDDGVEAEEAGSNHLGVHGSELIFPHNKSEGSIANYDDHSVRYFTDEEDKVLMEEIRKRHWMGIKGHSIYEAISKMPYFVSRRRTPASLRERMRTLKYKVGYVYKVDKKNRLLKDASGSYIKTTQITSKLTPYTAEDDLLLCKTIYLKLDIVTDDKGFESVVFPTNFFDKFAIVYDTHTPESWRQRYKNYLSIFGIANYIKYYIMEVKNDEEPLPANIANKEWLQARKHIKKTDCPRLYFPNIPQENEFIDENLYYIALPAYDTKIFEFDNPFRNIKKEESDGFSPEPIPSSSAGTTEMVNDTEKAIGVATAGSDYNGFVDEPTTEFIEVAFKKSYRKYGPPINLASVSDKKALITTFKKIFSVQGEKLAPRELSKRLLEEGVQEYYTVFLIYRCNSIKSLVQESLLNYVETDGAELLVMKPGIWSNKCIEMFEKHDPKLEKILKSYHGEKGYVKQARWLKTSKMDFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NE43</accession>
    <name>A0A1E3NE43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_74642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015514.1">
        <property type="nucleotide sequence ID" value="XM_019164830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NE43"/>
    <dbReference type="STRING" id="763406.A0A1E3NE43"/>
    <dbReference type="GeneID" id="30181517"/>
    <dbReference type="OrthoDB" id="432528at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="386"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="646" mass="74525" checksum="D873CDB93E6C4D51" modified="2017-01-18" version="1">MEGETSVQLKGSPPPYRINSTYCQFTRRGKPYLFVFGGYDDTNENFDDRIYLLDVQGQTWYPPHSTGVYRNGSSCVCLDDTGNVVIVGGLLQEDEYLECLERSNNVKSPYQDCFLLIFNIESYLFSREWNEKFKESLISEGEVGQTNWTQMNRLERHSICQSRSNHKIYISGGVISDLPYGMQKYMYILDYQKFTVEKVEFCKKIEHEIIEVGNKIWSFGGINETMKYSFMNIQTYDLQDHTVDEINLNINDNQMFYQNNKKRAGRSVNILGFKKFFYNQISITQILITDLINLRFVVLNIETHKMYHVPIQIANLNWLFVLTHNDNLSIIGGTELDDTVDEVAYLDYLVNIPLVTFGRLNNESGTSEEETLLGKLQKAYKDEKFVDFQIKSNDNKVISVHKLYLLIKWPYFEKMIMSGMSESHTNQMFIDEPYVNVKLLIDFLYNSEFPHMKVHDLLSFAHLVELYDLKDLKSLLVNRIYLSKLPINQLVEFWQLSDIINSKFLKKYLQSMIYRYWGYIVRLPSFQSLEKEQMLELFSSLSVESQIVTGNSPAKLVGNTSSSSALTPTPEVLKTSIPGSVPQNNSGQSVPFAYPDYNDTTIVTPEIDPTLTFDIFRSPGVLDCWTPLEPAISNDETSSSASFMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEL3</accession>
    <name>A0A1E3NEL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_36638</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015672.1">
        <property type="nucleotide sequence ID" value="XM_019163022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEL3"/>
    <dbReference type="STRING" id="763406.A0A1E3NEL3"/>
    <dbReference type="GeneID" id="30179709"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008989">
        <property type="term" value="F:rRNA (guanine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="10">
        <location>
            <begin position="82"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ44559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="359" mass="40883" checksum="41A831D7F3D4AA7D" modified="2017-01-18" version="1">MPAYVPKKAWERDGQDKEKWFKRKHAHHHILEKPHREQMEQRYEGIERKKRRERMEYVRQDEETRYYKDVLKQRRSNALIDHLFGTNAVLAALKSNKRKRFGKLYLHSPKDTDKFSEIMELAKELNINVKDSTKQELNILTDNAVHNGIVLETRPMEIETIKSMGSEVTEDNFSVRVLNDIVGDSSTEEIAVNSYGKRFPLGLYLDEISDPHNVGAILRSAYFLGVDFVVFSERNCAPLSPVVAKSSSGAIEFSKLFKVGKPLTFFDESKKNGWVFISTVAPNDSRNKSKQVDTDFISDILQKSPVILVVGSEGTGIRTNLINKSEYLVAVTNGREMNECVDSLNVSVATALLISKILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NER4</accession>
    <name>A0A1E3NER4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="12">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_60310</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="12">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="12"/>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015750.1">
        <property type="nucleotide sequence ID" value="XM_019163427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NER4"/>
    <dbReference type="STRING" id="763406.A0A1E3NER4"/>
    <dbReference type="GeneID" id="30180114"/>
    <dbReference type="OrthoDB" id="1637982at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit Rieske, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.270">
        <property type="entry name" value="Ubiquinol cytochrome reductase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037008">
        <property type="entry name" value="bc1_Rieske_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004192">
        <property type="entry name" value="Rieske_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02921">
        <property type="entry name" value="UCR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81502">
        <property type="entry name" value="ISP transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rieske" evidence="14">
        <location>
            <begin position="146"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ44637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="216" mass="23419" checksum="6A1ACA93C8C1847A" modified="2017-01-18" version="1">MLSRLSIRSARSLGLAQATRPISATVPAFKSTYDSPDFTHYLNNSKGSSDKSRDFTYFMVGSYGVLGAAAAKSTVEAFLTTMSASADVLAMAKVEVKLGAIPLGKNVVVKWQGKPVFIRHRTPGEIEEANEVDISKLRDPQADSDRVKNPEWLVMLGICTHLGCVPIGEAGDFDGWFCPCHGSHYDISGRIRKGPAPLNLEVPQYEFADPETLVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NEU9</accession>
    <name>A0A1E3NEU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EngB-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_18077</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015788.1">
        <property type="nucleotide sequence ID" value="XM_019161869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NEU9"/>
    <dbReference type="STRING" id="763406.A0A1E3NEU9"/>
    <dbReference type="GeneID" id="30178556"/>
    <dbReference type="OrthoDB" id="391988at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052279">
        <property type="entry name" value="EngB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498:SF1">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EngB-type G" evidence="6">
        <location>
            <begin position="124"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ44675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="353" mass="40132" checksum="F156A8CD12AACFF7" modified="2017-01-18" version="1">MRSCHSRLLGGTQLWQRYVSTVPHEKREILNLKALLAKSEEAELANKKIEVPKQNKAKKTDPHDNYGLEIMKELAKLTALPLKWKASHKEYTSIAGFLTLAHVALEWSIYDYRKIPTMEVAGKELPEVIFLGRCNVGKSSLINALLSKKKDAVVQPYARVKNQAGYTPCLNFYNVGGRFRLVDCPGYGVKGREWQGKLVFEYLQKRGNLQNTYLILDAEVGLNAYDELLLQNLTDVGVRFDIIFNKIDKIPRDKRKARILDLVTTGFIGGLSMTPRCYAVSSNEQSRTGITEVMVSVIESCGIWQDGEAGRLVAAKKKHVVRAEVARQQYKRGKKEHKELQRKQRENDQRLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF06</accession>
    <name>A0A1E3NF06_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_74428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015834.1">
        <property type="nucleotide sequence ID" value="XM_019164767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF06"/>
    <dbReference type="STRING" id="763406.A0A1E3NF06"/>
    <dbReference type="GeneID" id="30181454"/>
    <dbReference type="OrthoDB" id="5554402at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019419">
        <property type="entry name" value="AIM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28177">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28177:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10315">
        <property type="entry name" value="Aim19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ44721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="162" mass="16896" checksum="527C7E403D99297A" modified="2017-01-18" version="1">MSLAASDVQPPAVGDKNPKQLAAQWGVSPVPAWAFSAALLLSPALRPQFPVLNNPNAGGAGGANTGAGGMSSSMFRSAKAPLAAYPSNIQVLVFSSFIGLGGFMCYDHDPTNGAAVAGCWSLLYFLTNVKRSMWNLKVYPKALTSLALVNSAIYSAKYLNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NF99</accession>
    <name>A0A1E3NF99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Partial AB-hydrolase lipase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_36735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454006">
        <property type="protein sequence ID" value="ODQ44802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015915.1">
        <property type="nucleotide sequence ID" value="XM_019163033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NF99"/>
    <dbReference type="STRING" id="763406.A0A1E3NF99"/>
    <dbReference type="GeneID" id="30179720"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="2">
        <location>
            <begin position="95"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ44802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="450" mass="51472" checksum="F5DBD1E9F3D79A35" modified="2017-01-18" version="1">MLDSETSAGLSVESRGARRQWPVAVTVVAVGFTKVTSAVCSFLFIGFIFQFAVVHYVYHRYYLREAGRVWSAEEGYTEAYPVAPEGMRVTGDLGYYAQLLGLRLDEYEATTEDGFVITVQRLRDPKHGPDTLASGTLRPVLLVHGLMQSSGSFVTGGYKSLAYLLVQNGYDVWLGNNRCGFRPRHTHYGSNDPRMWDWDLHEMSQYDLPAMLRQIRAVKTNYAGKVSLVAHSQGTTQTVILISGQHRHPCAEDIDKCVLLAPAVYGGPLLNSKLFIKFMRFLPDGIYESFFGMNSFMPILMYLRQYTYKLPAFGLTSYMVFSYLFDWNDYLWDVRLRRYHFIFSPVFVSVKLMKWWLRSKHGRGFSTDKPIIDDPNAWFTKSTPDIFLVIGGKDDLVNGDLFVDRLERLEPDMTGRWSYVKVPEYSHLDVLWADDLLDRVGDALLEFLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFJ3</accession>
    <name>A0A1E3NFJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_154994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016013.1">
        <property type="nucleotide sequence ID" value="XM_019160852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFJ3"/>
    <dbReference type="GeneID" id="30177539"/>
    <dbReference type="OrthoDB" id="3997138at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF2">
        <property type="entry name" value="PH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="189" mass="21410" checksum="671DB424D2760929" modified="2017-01-18" version="1">MRIKSMCYWMDESIFGLKVSSFLNRLFGARESAEIQRDYTNENLNQIDGETAIEARANGVSAHNPSVTAYKSRRVGFGFGPLLQIVPFIGNYVVLTINMWIFCCMVTLGLGLDVSWNKGHVKVRRNPEQHFLGLKDMGTMIFNIVVDFGIGFIPFVGMFVMIIHRSSSRNLTVFWKSLDKKYAAKPKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFP2</accession>
    <name>A0A1E3NFP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_73733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ44932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016045.1">
        <property type="nucleotide sequence ID" value="XM_019164567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFP2"/>
    <dbReference type="STRING" id="763406.A0A1E3NFP2"/>
    <dbReference type="GeneID" id="30181254"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF1">
        <property type="entry name" value="REDUCED VIABILITY UPON STARVATION PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="255"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ44932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="315" mass="36741" checksum="F0793A5EAFFCE81A" modified="2017-01-18" version="1">MDGLTKAQIQHAKEAIQHYEGLQDAIRRHEASINEFTGHLFLMLVKFEEIIGAERVQVSPIVKPIQADVATRVSQFKSRIDHVLTDYLKLVKNIQKVFKEKGYLELDLRKYTSDNKKLKEKPQLLDKEYKRIFENQRKIEESREKLDNLNNVLKAELPSFLVLSNEIAKCISVIIFFHLHDLYKSMFDSFSLAKRFFPNIDLNNIYFENNTRKVKEVQKSICERLESLQMFNHYSNLYNVTPSEDAGKELVLCLKSSKLGKALYSFEGERRTDLSFSKGDYVTVLEESPTGWWKGRLVKNGATGNFPFNYFSFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFQ8</accession>
    <name>A0A1E3NFQ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_18317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454007">
        <property type="protein sequence ID" value="ODQ44408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019015521.1">
        <property type="nucleotide sequence ID" value="XM_019161947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFQ8"/>
    <dbReference type="STRING" id="763406.A0A1E3NFQ8"/>
    <dbReference type="GeneID" id="30178634"/>
    <dbReference type="OrthoDB" id="337660at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ44408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="371" mass="42238" checksum="783694A8F2D892ED" modified="2017-01-18" version="1">MDTKKEKNIVLKIGARTLECGLEDSSKPLGKFNVHHFAELQTFGVDSTAYDHSLIPDSASEKVTKEFRLQSMAKNDQEHFRHLFFPDTLIYEDLSLFLQSLKIHLRKVLNKIFYKCGISTINSKLILVQNSTFPKVYNKLLTEILINDLLMRAVVILPTPLMVSIASGSSFALIIDIGWSFTTVVPVFDYRVLDNLLTFTNRAGSRLHYELLSELREEGFDIESISFRELENTISLLDSLVCTSNDELIACGRFLVRKKIFTDAIEKLYLSFDSSVQYEDNEIPIIKLATDLIDKRLPIDLRKILSSRTIVTGGISNLKDFRQTLLARIDEAVAQQLHGIETLGDWAGASIYSTLMKHQKKSPDLWEIRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NFZ2</accession>
    <name>A0A1E3NFZ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_17551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016164.1">
        <property type="nucleotide sequence ID" value="XM_019161641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NFZ2"/>
    <dbReference type="GeneID" id="30178328"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ45051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="79" mass="8625" checksum="C0A7DF2278CA2B40" modified="2017-01-18" version="1" fragment="single">MQYTQNCLNKPQPTLRSICANGIWPSSPLTSRRITVRSQPVQARLALLVFSQTRIVSSVPGKGAFYCCAAKSVDVKDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG55</accession>
    <name>A0A1E3NG55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_165571</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016179.1">
        <property type="nucleotide sequence ID" value="XM_019161255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG55"/>
    <dbReference type="STRING" id="763406.A0A1E3NG55"/>
    <dbReference type="GeneID" id="30177942"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="145"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ45066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="305" mass="36094" checksum="2B5CD0FB7EB5FB86" modified="2017-01-18" version="1">MSNVFGSNSIDIPSKESIWQVFQTVKDIPGLNLVEKAWASYYVYMDNDLFATGLLFFLLHEVMYFGRCLPWYIIDRTPYFKKYKIQPNIYPSDKEQWECTKSVLKSHFFVEALPIWFFHPICEKIGVSYDVPFPSWSKIALQVSIFFFLEDAWHYWFHRGIHYGSFYRIIHKQHHRYAAPFGLAAEYAHPIEVMLLGFGTVGIPIVWCYFTRDLHLFTICIWITLRLFQAIDAHSGYEFPISLHHFLLIWAGADHHDEHHHHFIGNYASSFRLWDYFLDTEAGDTAKKTREKHSKTRAELMAQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NG74</accession>
    <name>A0A1E3NG74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/pantothenate metabolism flavoprotein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_73872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPC synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016199.1">
        <property type="nucleotide sequence ID" value="XM_019164615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NG74"/>
    <dbReference type="STRING" id="763406.A0A1E3NG74"/>
    <dbReference type="GeneID" id="30181302"/>
    <dbReference type="OrthoDB" id="70224at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990181">
        <property type="term" value="P:acetyl-CoA biosynthetic process from pantothenate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10300:FF:000002">
        <property type="entry name" value="Phosphopantothenate--cysteine ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="2">
        <location>
            <begin position="74"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="2">
        <location>
            <begin position="206"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ45086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="351" mass="39887" checksum="B97ABCCEBD2666A1" modified="2017-01-18" version="1">MATRENNEKRLLSRVATSVPDAQSAIDRSLPEFPAATPIDEDSYFRLHKPPAYLSQISTGVASFINENHGRRRKIVLVTSGGTTVPLENNTVRFIDNFSAGTRGATSAEYFLEQGYSVIFLHREFSLLPYSRHFSHTTNCLLDHLQFSQDGERVEVDGVLQKDLLSVLAQYQKVKANNMLLLVPFTTVHQYLFTLKLIAQELSVIGSDALFYLAAAVSDFFLPTSRIPEHKIQSQEHGGKLVVDLEPVPKFLRRLVDSWAPIAMIVSFKLETDNTILIKKARTALERYNHQLVIGNLLQTRKYEVVFVTPDNEHWIRLSKDAAEHKVEIESLIIPRIVAMHKTWIESRETM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGK9</accession>
    <name>A0A1E3NGK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_36403</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016362.1">
        <property type="nucleotide sequence ID" value="XM_019163003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGK9"/>
    <dbReference type="STRING" id="763406.A0A1E3NGK9"/>
    <dbReference type="GeneID" id="30179690"/>
    <dbReference type="OrthoDB" id="1107506at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00926">
        <property type="entry name" value="Cyt_c_Oxidase_VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003213">
        <property type="entry name" value="Cyt_c_oxidase_su6B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11387">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000278">
        <property type="entry name" value="Cyt_c_oxidase_6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ45249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="83" mass="9733" checksum="16DB1BAC0F38AE99" modified="2017-01-18" version="1">MADYKLQTPGFDARFPQMNQTKHCFQSYLDYHKCVAIKGEDFAPCKVFWQTFQSLCPGDWVETWDDQRANGTFPGDLSIERFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGU5</accession>
    <name>A0A1E3NGU5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_170610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016464.1">
        <property type="nucleotide sequence ID" value="XM_019161447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGU5"/>
    <dbReference type="STRING" id="763406.A0A1E3NGU5"/>
    <dbReference type="GeneID" id="30178134"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="26"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="204" mass="23052" checksum="D944C6C44FB5285C" modified="2017-01-18" version="1">MSGEQQAATNIDQDLQQSRYGKYSNPQLQKDVKGWITAILGDEADRAVLEQEELIESLKDGRVLCALVNRTFGDSTLKYKPSRMAFIQMENIEKFLNFCKLQGVAQDELFQTVDLYEAKDPYQVVIALQSFSRLLSEKFPGKYPQMGPSISKRHERPKVPAKPKHLVMGQGGVPWSSIEYGYMNGSNQKTEGVVFGARRDIVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NGU7</accession>
    <name>A0A1E3NGU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ESCRT-II complex subunit VPS25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_27213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016419.1">
        <property type="nucleotide sequence ID" value="XM_019162488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NGU7"/>
    <dbReference type="STRING" id="763406.A0A1E3NGU7"/>
    <dbReference type="GeneID" id="30179175"/>
    <dbReference type="OrthoDB" id="245150at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000141">
        <property type="entry name" value="vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.570">
        <property type="entry name" value="Winged helix' DNA-binding domain. Chain C. Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008570">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014041">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05871">
        <property type="entry name" value="ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="186" mass="22124" checksum="5C4A53AFEE99D25D" modified="2017-01-18" version="1" fragment="single">FELPRIYDFPPFFTEQPNLDTYQSQLEQWKDVIMKYCEHHKVWALSIVGKPINSENNDAEDDDFSIFKNDKIKRTASNEFIAKIYDYLIEQKSAEWIDPKSRNHGILIYWHSLEEWSQLLYDWIDNTGQQGTILTVYELRRGDLALNREFYGMNYHMMVRVLEKLVKQGKATIMKDDENNIGGVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NH77</accession>
    <name>A0A1E3NH77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_74245</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016600.1">
        <property type="nucleotide sequence ID" value="XM_019164720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NH77"/>
    <dbReference type="STRING" id="763406.A0A1E3NH77"/>
    <dbReference type="GeneID" id="30181407"/>
    <dbReference type="OrthoDB" id="2192830at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="383"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="212"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="266"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ45487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="446" mass="50320" checksum="4B86699CAE7B9AD0" modified="2017-01-18" version="1">MVHGNYICIHCGSSIPALYERYGGGHIRLSLCQYCNNVADKYVEYDNVLLFIDLILIKPCAYRHTIYNVFLSSSSEQESFEKKNRTIGSLVSTSKRQKVSRAVTPQISLSSSVDALMSPGEGQKSFRPDTSLIPLVFRLGILMLLFEVYVTWAYHEKSFMEKADHTSLIISLVLKGPIQYIYFLSTTLLQNILLCVSLSYYGLRWLPFPTSKPATSGDTPTSTDTSVNSTPFSSRSPSPSPVSPKFNSPRMSHLRLPPHQPREKQSTASFNSTSQLKSKQPTIAGEYVPQQYPDSLLMSSKPMFSRICGIMLTTVLISNIIKLFPIVMLIWPYDPPILHATRLLVRIVHIFLLIEALYIVLIDPKNTKRKSRRRYSSSSSNDYYRVTAVVLVSELTRIVVSHIIIVFVASAIWGVSVREIGLDDWKMLKVGIRMLEELGEYILGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHK7</accession>
    <name>A0A1E3NHK7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_12073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016725.1">
        <property type="nucleotide sequence ID" value="XM_019159877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHK7"/>
    <dbReference type="GeneID" id="30176564"/>
    <dbReference type="OrthoDB" id="10638401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="241" mass="27245" checksum="F57679C449E011DB" modified="2017-01-18" version="1">MSYIYQRRHVPTAAFNTAHSDPETVGSPLSYSDCDTEDQYYCSDNQQQQQQQQQQQQQQQQQHASSPSSRIESLLASMSNHIHNKKELVNLIILIIYENFEMKFNLGLQDTEVSGFCHLINLLVLKCGLSLSSLTKNLLILEKILLHHSSMYSSPKIPFNLLKRMVLTSFIIGTSNVDSNHQVVVNFDKWCLLTGLPSDLLKHDVCSFPDSVSIGSQISDGEVASRNAMLQLQVQKYVKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHK8</accession>
    <name>A0A1E3NHK8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cyclin-dependent kinase 8</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PICMEDRAFT_35339</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016722.1">
        <property type="nucleotide sequence ID" value="XM_019162935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NHK8"/>
    <dbReference type="STRING" id="763406.A0A1E3NHK8"/>
    <dbReference type="GeneID" id="30179622"/>
    <dbReference type="OrthoDB" id="6284126at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990508">
        <property type="term" value="C:CKM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060258">
        <property type="term" value="P:negative regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07842">
        <property type="entry name" value="STKc_CDK8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000408">
        <property type="entry name" value="Serine/threonine-protein kinase SSN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF495">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="27"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ45609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="451" mass="51238" checksum="12CEDB1BA5F2F7E1" modified="2017-01-18" version="1">MAPNNFFTMGPYRARKDKARLSVIDKYDVQGYIAAGTYGKVYKAVGKLGNDVDKLYAIKKFKSDSKDNEVMHYTGISQSAIREMSLCKEVKHKNISTLREIILERKCIYMVFEFAEHDLLQIIHYHSHPEMKPISQSTLKSAMFQILQGLSFLHQNWILHRDLKPANIMVTHDGVIKIGDLGLARKFSNQLQTLYTGDKVVVTIWYRAPELLLGARHYTPAVDLWAVGCILAELLSLRPLFKGEETKMDNKKHMPFQENQLLKILQILGTPTLYDWPSLNNYPEYPQLQRFTLFPSNIKAWYANNGGSDPNCFDLLSKLLIYDPAVRINSLDAMSHKWFLQSPKPVQNIFEGSTMKYPRRKIHKGDSDILGGANNSIYGGAVSNVNANSRANNKRKIPNSNNANIGNIGSINMNNMSNMSNSQINQKRNYAFMSGRGSNSSGFEDNSKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NHX1</accession>
    <name>A0A1E3NHX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_73229</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016827.1">
        <property type="nucleotide sequence ID" value="XM_019164415.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NHX1"/>
    <dbReference type="GeneID" id="30181102"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="8">
        <location>
            <begin position="345"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="8">
        <location>
            <begin position="479"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="608"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="625"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="640"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="1357"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ45714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1414" mass="161913" checksum="1D3B79B1A972B840" modified="2017-01-18" version="1">MGKAVKGRRKFRENWDFNTNRFRKKFLLLKHLRDTRFKISDTMALALMDEETRNREAILNDLEPVVIPHSVYGPGVKKSVKTSRTNSESSSSLPQAFIPANLSLNTSLITDQTRGSNTATRSGSIEMCPAHVEYTLPTISELEETGLKEFYNSFLNSATWQISNLNVRPGDDHFIFWFMASSYIPLICSCSGPLSNLFSLLAIICPWKINKHNPSFEHDPFWCYLVNSISIVFALISNIFLLLNYRKKIRYTYCQVISISGWAIACVILTVLIVVYHVWFYRYHYDEDYIIGEGFWFAIITVTLHFTNFLMLLLNELGFLLKKYKPVFNIDQVQETLIIQTTAMAVWLIIGASILTRLINLGLGDSLYYCIVSVVTIGEQDVVSTTDVAAQTVSAIWIVFGLVMFGLIVTSIRKMMIDFSSSTLYWHRLERLRRKSLETHQNETRISSTNQESFVLIKNIAKWAYTIQGVAELTTSIIIFMFTLMVGAMALSILESWPYKTTVYFCFFNLMTLGQGTEAPTTPGGKAIFCAWALAAIPVMTILVSTSSDFVFSRLTVWEEVPFSDAVIEYCLSKSFLKSLGYFLKSRQLYALDKASVFEMKNSSMLKVDDTKNHGDDNNNDNVEDYINNNNNNINNNNDNNDKDNTHGNDRDVEMDNTPGIRISEFDRGKHDVFADVPVICHPADMLYNVILDSTGVDNFGFITSRSFVRSNTATIQLANYFREGAYVNPNIDQLHESRQTLGQHFKDLDSKFDVNEYEKAYDIYATGQNHGVIQKDLRIMTGFKKKNDFVLNKLSRIQVILLELRSALHKVCTNLDHEYSYQDWEILFNITQSEEAIEDNLYWIESRSPLAFPMHQPKYFTLHYMRHLELFLQQFAAEWDELPIDGENIADDNIVNVDIPEDNSTVHAAVTISTLVLWGIIYPLYFHPLSKVPGPTICALTSYYILYKTWNEERNRYVQELHEKYGSVVRIGPNEVDINDVAYLKEIYIGDYDKTSFYSQFVNYGQPNTFSVTTKKEHTQSRKVSHKFYSKSNICSHDIMPKIEKVMSDTLSAFDRYNGKSINVFVLFCDMAMDAVTSFSFGINNYKSLLSDPFGNGSIIVNDFGLQSSSWFWVTHMPSFYDLVVSKKVVEASKRCYDWIENQFEYSMGRLSNDEEKQEKTLLSVYFDSVPSSTEAKQKSQVFDIMKTKSEFFDHIAAGHVTTGTTLSYLFYELAKYPEMQEKLRSEILSKINNNESTAPSNYVPFKYSLVDNEDLLPYMNAVILETFRVHAAIPGEEPRVVPAKGMIFRGNSTVPACKIPAGTTVVMQPWSLHRVSTLFPDPDRFDPERWLDVSPAQLKQMKGNLMHFGAGARMCIGMNLATTEIKIIVSSLMARYRIELVDNFDYDYDGAMLDIYTTLPRSEKMMLRFTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI14</accession>
    <name>A0A1E3NI14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">aromatic-amino-acid transaminase</fullName>
            <ecNumber evidence="9">2.6.1.57</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73266</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an aromatic L-alpha-amino acid + 2-oxoglutarate = an aromatic oxo-acid + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:17533"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:73309"/>
            <dbReference type="ChEBI" id="CHEBI:84824"/>
            <dbReference type="EC" id="2.6.1.57"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.57" evidence="9"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016874.1">
        <property type="nucleotide sequence ID" value="XM_019164428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI14"/>
    <dbReference type="STRING" id="763406.A0A1E3NI14"/>
    <dbReference type="GeneID" id="30181115"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000074">
        <property type="entry name" value="Aromatic amino acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF21">
        <property type="entry name" value="AROMATIC_AMINOADIPATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="95"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="474" mass="53523" checksum="653BD0F810E29ED9" modified="2017-01-18" version="1">MVKDLSHLLSEESKGRKPSPLKSAFKYYGKEDIVFLGGGLPLADYFPWEKIVAYSPEPPFEDGIGAQPSVTGKSTISEVHKHKVEEFDIPLARSLQYGHTQGNPELIDFLKEHTKMIHNVPYEEWDLCLTIGNTQSWEACIRSFCNPGDSIFIEEYTFPSAVETARNLGVNFVPMKMDAFGIIPSALAELLENWDKSKPLPKLLYTICTGQNPTGSSLGYERRKELYDLACKYDFVIIEDEPYYFLQMTEYQSGKEEEIKTPSHEEFLKSLVKSFISMDTEGRVLRLDSFSKVLAPGTRVGWIVGQAALLERFVRLHEVSIQAPSGFSQTIVQALLGRWGQSGYVDWLIAMRKEYSIKRDFTIKCLEKYMPKEVVSWTPPIAGMFFTILLDASKHPDFATKYDSDPLKVEDAVYEQGLKDGCLMIPGSWFVAPNRKNTSGTTFFYRGTYAAVPLDTLGVGLERFAKAVKSEFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI34</accession>
    <name>A0A1E3NI34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Histone-lysine N-methyltransferase, H3 lysine-4 specific</fullName>
            <ecNumber evidence="3 14">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_144560</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic component of the COMPASS (Set1C) complex that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3. COMPASS recognizes ubiquitinated H2B on one face of the nucleosome which stimulates the methylation of H3 on the opposing face.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60272"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60268"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the COMPASS (Set1C) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="3 14"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016859.1">
        <property type="nucleotide sequence ID" value="XM_019160381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI34"/>
    <dbReference type="STRING" id="763406.A0A1E3NI34"/>
    <dbReference type="GeneID" id="30177068"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024657">
        <property type="entry name" value="COMPASS_Set1_N-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017111">
        <property type="entry name" value="Set1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048669">
        <property type="entry name" value="SET1_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024636">
        <property type="entry name" value="SET_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11764">
        <property type="entry name" value="N-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21569">
        <property type="entry name" value="SET1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11767">
        <property type="entry name" value="SET_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037104">
        <property type="entry name" value="Histone_H3-K4_mtfrase_Set1_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01291">
        <property type="entry name" value="N-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51572">
        <property type="entry name" value="SAM_MT43_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="258"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="934"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="20">
        <location>
            <begin position="1060"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="62"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="633"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="356"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ45746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1076" mass="123028" checksum="413B57C70A8D4753" modified="2017-01-18" version="1">MAYRGGHYKKGYGYPPNSKGDYGGPQQRYDHGGRLSNGYSTDYPNEYSSDYANGYGAGYYDYYDDGYSIQPPPQQPPPPPLQRDPPSSLYESQSSSYYNSYSGYHSHRLPRHHSSGDDHTATNYPPSSSSAPSPSPKIVSASGTRRAHSRQGIHAPIVKTYALGDPKQGNFISQLAPLDEGQILEAKSFKTIHLKTENRKKNAIEHVQGLSKTVVDPRLNNSASYLKSLSKSFSKPVEKIYLPTFKFDSHSLGEKPSNEILIWNLHSTTTSLIIKNNFSQYGPVSEVKVIDDPSTAVPLGMCLLSFDGKVEQAHSTALKTVEMANKRLVIQGRYIRCGLNVKNQLYDEIYNKTMDVRKEKLRKQKVEEAKKEEKLKIERLREEKLAKEEQIRLAKEARMNREKYNKLLPSGPSSKGLTSSSKVSVHDRHIFPASSFSLSYKFQKYISNRPYIFISDKYVSSMHVSSDQLRRFLSRYNISRILQQRNGFYLVFNKLSEAMQCFDTEDGKKLMNYTVYMTLYVPDDQIDETRVGKAGNVKLAQKQVVKELNAYLLKDIREKVIGKMILDVLDTDEIAKLAQESKKKKDEERKLMKQQEKERSIAKDENTIRVIKRLDLNVVRKSVKRAFIPMSHSLNKGHDISDDDEEEDDEDEDEDEDASETDGTPIDDTLNAKDGSKSIKKRKPETHISKLDKKKIKFQENIESEEEDDEVDFDDNDEIKVEAEQTDITPPSETETIGKTIDNVFQPSTALPGPVFDDHLEPFSPTLQFLKNSIKTDEDFQILSNLCKGLNFEKPIKSVAFFAWEHMEAMKESKELREEKEDEGDDAEVFDFAEEVLNNKELRNSSGCFRTEGYHKIPDKYKREYLLHRRNLTNLNPVKHEDDDEANTLNYNNAQSSRVNRANTRRFVADISAQKQIIGETDLLDLNQLNKRKKPVQFARSAIHNWGLYALEPITAGEMIIEYVGERIRQQVAELREKKYLRSGIGSSYLFRIDENTVIDASKRGGIARFINHCCEPSCTAKIIKVGGKKRIVIYALRDVKRNEELTYDYKFEREINDEERIPCLCGAPGCKGYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI75</accession>
    <name>A0A1E3NI75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-5-taurinomethyluridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_26650</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="3"/>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016156.1">
        <property type="nucleotide sequence ID" value="XM_019162456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI75"/>
    <dbReference type="STRING" id="763406.A0A1E3NI75"/>
    <dbReference type="GeneID" id="30179143"/>
    <dbReference type="OrthoDB" id="3685at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103016">
        <property type="term" value="F:tRNA-uridine 2-sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990799">
        <property type="term" value="P:mitochondrial tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.280:FF:000001">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000115">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001138">
        <property type="entry name" value="PRK00143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like central" evidence="13">
        <location>
            <begin position="206"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like C-terminal" evidence="12">
        <location>
            <begin position="312"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ45043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="368" mass="41966" checksum="024CA6E4E80086DB" modified="2017-01-18" version="1" fragment="single">MSSGVDSSVTALMFAQRYRNVRGVFMANWSPDRMADSSSTCTVDTDWLQVRATCEQLQIPCERVNFEREYWTQVFEPMLEQYERGYTPNPDIGCNRYVKFGKMVEHLARRFEGSGRRWWLVTGHYARILRDEAGEHRLLRAQYRQKDQSYYLARMPHDVLARVLMPLGHYTKPQVRQIAREYDLVTKDKPDSQGLCFVSQAGKFADFLGDYLRPNPGNIVTADGHVWGRHKGLWTATIGQRSGISMPQGDPRYKGVWLVADKNVARNELVIARKDDREAFYRSTVAVDPASWYWQGARLGWDAVQHLLRTGANVNCQIRSLQTPNTVKSVSCHDGVLRVQLAEEIFGIAAGQTLAIYHDDVLLGSGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NI85</accession>
    <name>A0A1E3NI85_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_17761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016391.1">
        <property type="nucleotide sequence ID" value="XM_019161730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NI85"/>
    <dbReference type="STRING" id="763406.A0A1E3NI85"/>
    <dbReference type="GeneID" id="30178417"/>
    <dbReference type="OrthoDB" id="5561579at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019727">
        <property type="entry name" value="ATP_synth_F0_fsu_mt_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28161">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28161:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10791">
        <property type="entry name" value="F1F0-ATPsyn_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ45278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="97" mass="11033" checksum="7783DE60F4DC438D" modified="2017-01-18" version="1">MSNVFRRSLTTLIPPKIASPTNLSSNPAAKRMQSIVAFYSKLPRGTATFEAPKTPFAIYREAYRNKGSPVLHFALGFLFLGYGLEYYFHLSHEKEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NIB5</accession>
    <name>A0A1E3NIB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_73869</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMARCC family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454005">
        <property type="protein sequence ID" value="ODQ45083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016196.1">
        <property type="nucleotide sequence ID" value="XM_019164612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NIB5"/>
    <dbReference type="STRING" id="763406.A0A1E3NIB5"/>
    <dbReference type="GeneID" id="30181299"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048858">
        <property type="term" value="P:cell projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381:SF1">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="9">
        <location>
            <begin position="388"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="564"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="530"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="746"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="535"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ45083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="863" mass="95138" checksum="62B58ADCFC6E81C8" modified="2017-01-18" version="1">MDEETPQKNVAEAVGEASESHPAQSDKQPRQEHETEPPNGTAEKREEEDRATSSVSASGTPEAAAADEEDESAGEKEAGIPEQSATAESVPQDTSETAENAAADEIIEPEKAAGEGNTAEQQGNTSNVAKDGEGEALEETKMPEEKADVAKDTPAAVDQAKPTEDGIADDGADDGADAGADGASNQATGGEPQAAPKKKPASDPSEAAAVLAEPAQAEEKVKSDSPEDSKDSAEKQKFQGGSGPNSGSNNDMDDLFDDSSLPLLDNDDPDDGDFVMQNNDENNGDENHNEEGVDSKTVAIGDLSAGETEDKDEAALEEEKEEKENNEIVDKIGTGIDDKPENIEQGSPETVNDLQESAENLSNPQVGTSPSKEPEVSMSSLGRQTHTIVLPSYSSWFNLSTIHEIERESLPEFFQTVNKNKTAEIYMKYRNFMINSYRLNPNDYLSYTAVRRNLIGDAGSLLRLHKFLTKWGLINYQVNPETKPKQTEPPYTGDFVIDYDTPRGMFPFESYKPPTTFPDLKKLRAILAPPSTGADEEKQSTKDSEQKTPDSEPPLKKRKILKPDIDHNWDEISLQRLVEGVSKHKNDWYKVAEFVGGNKTPDECIIRFLQLPIEDKFLEDNKDVLGPLKYIPNLSFSPNDNPIMSTLSFLVNMVDTDVAVAASNRAIKVMDKKLEKKLNRFKDPEEGATPASDKDDALKDVKDAAVNTFGIIGARSHLFATFEEREMHKSLVNIIQHELKIVDMKLAKLTSLEKEFELQRKRLEKKSSELLEEKLGIFKYNNAATSKLLQVISLLESKDDYKEVDIGKIKNLITQSKEILYKPPRKQLNILEEGDFDESGENLNESVKPISFEAPMLYRYWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJD2</accession>
    <name>A0A1E3NJD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP proline-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_16169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017367.1">
        <property type="nucleotide sequence ID" value="XM_019161114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJD2"/>
    <dbReference type="STRING" id="763406.A0A1E3NJD2"/>
    <dbReference type="GeneID" id="30177801"/>
    <dbReference type="OrthoDB" id="10260794at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="315"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ46254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="494" mass="55796" checksum="0C56F4D09E08A1C6" modified="2017-01-18" version="1">MGRSSVTTQERRKSKNQLRRERAKLLKQQDLTSATKSISSAKVLPGQVRVEDTTAPASSAEDADTDEKNVFLEEAYKDIFEKFDLSKLPDTEQEEDDKQRTDNEVKTENHLLIGSDAEEEDEDSDDEGKRQGNEKKEVVKSDTNNEVETSVEDSNNLSKRKFKKLYSLPLAVLKGESKYPELIEWMDANSPDPRLHIYLKTLPNSVSIPSHWQSKKSFLSSKRGIERPPFELPEFIRNTGILEMRNTVEESENEQSTLKQRMRERVQPKSGQLDIDYNKLHDAFFKYQKKPPLLKFGELYTESTNNDDLVLKEKISHFKVGVLSPKLKHALGMVDEKGTLINRLPPWYYKMKEIGPPPSYPYMKISTSGKISFDNPNELNIGPPITTEHWGMLVNDLDASGDDELDEKDFQESNSNTQANNGFDRTKGDVLLTAFGEGPARSAPSIGQNSQAANDGKPKRLFTVLQSTRGGSNDGGNNKNSIFGSQTTSYRVPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJF7</accession>
    <name>A0A1E3NJF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Carbamoyl phosphate synthase arginine-specific large chain</fullName>
            <ecNumber evidence="14">6.3.4.16</ecNumber>
            <ecNumber evidence="5">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Ammonium-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Arginine-specific carbamoyl phosphate synthetase, ammonia chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Carbamoyl phosphate synthase arginine-specific large chain, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">PICMEDRAFT_16999</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Large subunit of the arginine-specific carbamoyl phosphate synthase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, hydrogencarbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CarB family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="14"/>
    <dbReference type="EC" id="6.3.5.5" evidence="5"/>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019016821.1">
        <property type="nucleotide sequence ID" value="XM_019161422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NJF7"/>
    <dbReference type="STRING" id="763406.A0A1E3NJF7"/>
    <dbReference type="GeneID" id="30178109"/>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000015">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="24">
        <location>
            <begin position="182"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="24">
        <location>
            <begin position="718"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="25">
        <location>
            <begin position="983"/>
            <end position="1138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00068891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="ODQ45708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1138" mass="125333" checksum="F1B8D6FBC9CDC3A8" modified="2017-01-18" version="1">MTRLISTPFKLRQIARLSSFKSVLRTQNKSYSTASYEGGNIVKKFKDDNGLHLVDVSKVLVIGSGGLSIGQAGEFDYSGSQAIKALKEENKKSILINPNIATNQTSHSLADEVYYLPVTPEYITYIIEREKPDGILLTFGGQTGLNVGVQLNKMGILEKYGVKVLGTPIKTLETSEDRDLFARALNEINIPIAESIAVNTVDDALAAAAKVGYPIIVRSAYALGGLGSGFANNESELRNLSAQSLSLAPQILVEKSLKGWKEVEYEVVRDRCDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEEYHMLRSAAIKIIRHLGVVGECNVQYCLQPDGLDYRVIEVNARLSRSSALASKATGYPLAYTAAKIGLGYTLPQLPNPVTKSTSANFEPSLDYIVTKIPRWDLAKFQHVKRDIGSAMKSVGEVMAIGRIFEESFQKAIRQVDPSFIGFQGGKFEDLDDTLANPTDRRWLAVGQALLHEGYSVDKVHELSKIDKWFLNKLMNIVKMQKEMEGLGSIEKIDEDVMSRAKKLGFSDKQIALAVGSDELSVRSLRKSYGITPFVKRIDTLAAEFPASTNYLYTTYNATCHDVEFQDQGTMVLGSGVYRIGSSVEFDWCAVSTARALRKAGKKTIMVNYNPETVSTDFDEVDRLYFEELSFERVMDIYELEHSEGIVVSVGGQLPQNIALKLQNTGAHILGTNPEDIDKAEDRHKFSSILDTLGVDQPEWKELTSVAAAEEFANAVGFPVLVRPSYVLSGAAMSVIRSQSELESKLLNAAEVSSEHPVVISKFIEGAQEIDIDAVAANGKVIVHAVSEHVENAGIHSGDATLVLPPQDLDDAIMGRLKEIADKVAAAWKITGPFNMQIIKAENAKTGVPDLKVIECNIRASRSFPFVSKVLGVNFIEVAARALLQDSTLKPVDLMGKKYDYVATKVPQFSFTRLAGADPFLGVEMSSTGEIACFGKDLIEAYWTTIQATMNFHLPLPPSGILFGGDLTKDFLGTVANRLGGLGYKYYATTPEVAQYLSEYTDVKVEVIEFPKTNKRELREVFQKYDIKGVFNLAKARAESLDDEDYVMRRNAIDFGIPLFNEPQTSMLFAECLKEKLPARLADLRKSEVVIPPEVRRWSEFIGGKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NJP8</accession>
    <name>A0A1E3NJP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">DNA topoisomerase 2</fullName>
            <ecNumber evidence="5 16">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PICMEDRAFT_51795</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 16">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5 16"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017419.1">
        <property type="nucleotide sequence ID" value="XM_019163158.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NJP8"/>
    <dbReference type="GeneID" id="30179845"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097047">
        <property type="term" value="C:DNA replication termination region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000019">
        <property type="term" value="P:regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097046">
        <property type="term" value="P:replication fork progression beyond termination site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009303">
        <property type="term" value="P:rRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13 15 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 16" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="18">
        <location>
            <begin position="499"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="19">
        <location>
            <begin position="752"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1232"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1304"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1250"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1273"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1316"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1331"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1398"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="15">
        <location>
            <position position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ODQ46306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1417" mass="159842" checksum="21836BCCD6AFDDC1" modified="2017-01-18" version="1">MSTGSGSDAYYSSDEEPATKKAKPSNSRAKPKSKTSASDQYQKLSQLEHILKRPDTYIGSVEKHSAEQWIFNDATKCMEKKVVSIVPGLFKIFDEILVNAADNKIRDPTMKKIDVVINAEENIISVKNDGKGIPIEIHDKERIYIPELIFGNLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSKEFTVHTADKSNGKEYNQTWRNNMSEKGTPKITAYKKQSEFTEIIFKPDLEKFGMTELDENILGVFKRRVYDVSGSVRGIKVTLNGELLKINNFKQYVELYVKAIEMAKEENPHVPVHIKTEGEENAESANDSEQNGSEPPPSMPEKLPTIVHEIVNDRWEIAFCISDGNFNQVSFVNSIATTVGGTHVDYVANMLVGKITDEIKKKNKKAIIKPFQIKSKMFLFINCLIENPAFTSQTKEYLTTQPSKFGGKRLELPDKFVKKVLSCGILDEVLDIAQANADKALKKNDGSRKSRITGYPKLEDANKAGTKDGYKCTLILTEGDSAKSLAVAGLAVVGRDFYGCFPLRGKLLNVREAGTDQIMKSGAIQAIKQIMGLQHKKHYGPDDIKGLRYGRIMIMADQDHDGSHIKGLIINFLETSFPGLLEIPNFLIEFITPIVKITIMSGPNRRKTIPFYSMPEYEHWRETEGTTCSYKQKYYKGLGTSSAPEMREYFGQLDKHLKKFDALKEEDVSSIDLAFSKKKADDRKEWLGAFQPGTYLDPTLTDIPISEFINKELILFSMADNVRSIPSVMDGFKPVQRKILYACYKRNLKDEIKVSQLQGYISEHTGYHHGEASLMQSIIGLAQDYVGSNNLNLLIPHGGFGSRAAGGKDAAAARYIFTELSKITKKIFNPLDNPLLKYLQDDEQTVEPEWYTPVLPMLLVNGCEGIGSGWSTSIPPFNPIDIIENIRLLMKGEEMKEMLPWFRGWEGTITRELNKDRWRLEGKIEEIDDNTLEITELPARMWTITMKEFLLNGLAGSDKQKAWIKDMEEDHGVGVKFIVKLSDEEMEKSRRTGLLERFKLISTISSSNMVAFDPNCKIKRYSNVNEIIQDYYYIRLDFYQKRKNYMVKEYSNQLEKISSQAKFVKLIIEGDLIVGNKQKKVLVEELKGLDFPGFDKNNEPVNLKHSSSVKVEEKDGTVDATGTESTGLVVVHKRNLSLLYDYLLGMPIWSLTRERYEALLRLQGEKEELLNTLLQKSPKDLWNEDIEEFLLEWEKFLKKDEENRSNLVSNGGEKNGTKRKRKAPEDSVEPKAKKPRANGKATMTKVKKEEIADAPKEETKDLNDFDSIFKEFTASSSAFKDGDDGIKETTVPPTAPSTTSAKSSSKKTTAKKATNGRKKTSLVSDDDEEGYSQSVDLTSGGEDEDDSVSGPARRSTRARRSKTPIQSYTLDSEEEAVSVNESDDNYSEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NK80</accession>
    <name>A0A1E3NK80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Altered inheritance of mitochondria protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_17219</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane raft</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ45968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017081.1">
        <property type="nucleotide sequence ID" value="XM_019161516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NK80"/>
    <dbReference type="STRING" id="763406.A0A1E3NK80"/>
    <dbReference type="GeneID" id="30178203"/>
    <dbReference type="OrthoDB" id="3998277at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031370">
        <property type="entry name" value="Aim3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17096">
        <property type="entry name" value="AIM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="89"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="318"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="581"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="663"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="757"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ45968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="805" mass="85113" checksum="97AAA3A043FCF49D" modified="2017-01-18" version="1">MSFWENNKGSIKSGGLAVLKGAGKATSAVGKAGYSAYKNHNNKKPGSKPEGEEEEEAAVESNSYGSMTYRDPKSFPPPPHHVGAGGQPGYTAAGGPAAGYQPQYPGYGQAQQQQQQQQNPAYGQQPQPYGQYGQPEYGQQTPAAPYGQSHQQQPPQPAAVPYGQPPASQYGQPQPAPANQYAQQPPASSYGQQPPGGQYPPSNPYVQQQPLAAPYGQSSANPYGQSSATPYGQAPPIANHYGQQPSASQYGQQPEQTQPLPQPPTPPYGQQPASSPYGQPVAPVYGQPEASLFGQQPAAPAYEQPTASPYGQPVATPYGQQQSPLPTPASQQQLPTHQQPPVAQQGSLPTPPDVLSYTNPYHSLPLAQPESSMPGYISQQSSNSVNVAPGAPAAQQAGANPYPAPPARTLPPVVSNPYQQQIGQSGANGIIPPAAYGQQQSSPPPPPVRDDQSLSSASSYGQYNQPPTADVSSRSSYYPAPPSRTATSEPLPPARTNTTSGAGGRDQAGGKPGEEVIVEPQFKTNLMDFDLKKFGAPPPRARLTKEEEIKYERRKGDANRLKQIKEQSLQKARGTAEKSISPYNSTFPTAAPSKSGSVEQLNSVEAETSYEVEAGSQSSFTQPAALARPEGQTEDSQPVPFHLPDISQFQPPPLAHRVPSVSDAYSSNSGHTAVLSTPQPVIEKHKPPKPPKKFPQEAISKADFPKPVASTPYTRPALSLPAGTSFEKPAKKTPPPKPAKISDTHSNSKKMPPPKPAKLGKPAKPTKPADLSTRSTTGEHNGEVESSAKGNHIRELQARLGNLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKB1</accession>
    <name>A0A1E3NKB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="10">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_72653</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017708.1">
        <property type="nucleotide sequence ID" value="XM_019164265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NKB1"/>
    <dbReference type="GeneID" id="30180952"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000069">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="30"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ46595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="120" mass="13755" checksum="2145F2D394A8D9B6" modified="2017-01-18" version="1">MSDLTTRPKSELSQNELQDLEDYEFNHGPLRVLAQAVQNSDSVLISLRNSHKLVARVKAFDRHCNMVLENVKEFWYEEGQGQGDVKSKTKPGNKVLRERFVSKMFLRGDSVVVVLKYNGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKJ6</accession>
    <name>A0A1E3NKJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear thiol peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PICMEDRAFT_16512</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="3"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017788.1">
        <property type="nucleotide sequence ID" value="XM_019161231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKJ6"/>
    <dbReference type="STRING" id="763406.A0A1E3NKJ6"/>
    <dbReference type="GeneID" id="30177918"/>
    <dbReference type="OrthoDB" id="338622at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000157">
        <property type="entry name" value="DOT5p Nuclear thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF23">
        <property type="entry name" value="PEROXIREDOXIN DOT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="100"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ODQ46675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="249" mass="27359" checksum="AF7611D9495E5C48" modified="2017-01-18" version="1">MSTAPRRSKRLEAIKDELAVSTEKPQPLKSEPVKKAASKGKVVKKENSKANGKTKAGKKDTKSATTESKGKSRSVKKEPIVIEDDEKAKEEEDEEAKTELEEGDILPKNLELETQDSKKINLLKYAKDAHILVIFAYPKASTPGCTRQAQGFRDEYSKLNGDLKASVLGLSADNAAAQTKFKEKQNLPYDLLCDPSKKLITLLGCKKTPSGTIRSHFIFVDGVLKVKKVKISPEASFKGALEQVTELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NKY0</accession>
    <name>A0A1E3NKY0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_11774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017918.1">
        <property type="nucleotide sequence ID" value="XM_019159839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NKY0"/>
    <dbReference type="STRING" id="763406.A0A1E3NKY0"/>
    <dbReference type="GeneID" id="30176526"/>
    <dbReference type="OrthoDB" id="438641at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="502" mass="57144" checksum="C4DE628E3E67D893" modified="2017-01-18" version="1">MENCTIPTNVVTDTLNPILKKILSKSKSTHLIRLDTVTNEIKLKVDKPHLGLIYLESPYIAIPSFDLLKLIERGKCCGLCGGVLKIYDNGKMKDDGNKSTRQKQTHIEKHERYINGLDCSECEVRWCDKQCKDLDFKHMLLNHRPSNKSTSHSFLDVAGNEKDIFYFDKWTKLKNLLVSENLDVVYNTFMCIIQIYYDETLVQGFESLKCVVCDDESQLELYINSWNILKVIDLKTLWQSFTGCFKKLNMSYLSFLKYVIIFKLNNYNGSIYLIFSAMTKSDSVEPNVKVEYYDGATQYDYEEFSVRTGEDNAVHMIKEHVVDRASVKPIYTNRSSGILDKKVIQVSNTARVSNTAPLVLPDEDFSNPADVEDDEIEFLSDGESDTKIPKIVLPVLPSYTQKTTNSKIRAASFTSSGASFGEGIIKYNRDQIREMLENMSHSMRLDEESSDEAEDAIDDEISSSVGSSISRNIVSMVKNLQVNNAASQRRKSVKFEEKVTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL11</accession>
    <name>A0A1E3NL11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_24230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017889.1">
        <property type="nucleotide sequence ID" value="XM_019162344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL11"/>
    <dbReference type="STRING" id="763406.A0A1E3NL11"/>
    <dbReference type="GeneID" id="30179031"/>
    <dbReference type="OrthoDB" id="271862at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="557"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="351"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="838" mass="96302" checksum="4ED903C4BC9D83BA" modified="2017-01-18" version="1" fragment="single">YQHSNHSMDSSSDDKLVLSFSLPYQYFIKPFNVHREFLIPSDWRLNNIVNSPNPNLANNDSFNDNSLIFDNLKNLGLVVNQLNESYASFGIIIILNEFRISDYKITISIIGTNRLKKEMLDIRLQILQNFSQISKSMIVLDPVKNKHCFNMDNEHDLKMELISFISSVSSFNKCSIYIEKSDNFAFSISIIGYRDQVKVTENKINLFIDDMNPQFYSDYLELSSVSILPLICGTDLFTLKRIIKETNCNIYLPNLLPNLYYNNSLEVELGNPKIFLAGLRSEVLLAKKCLSDMILKYTKTPFIKQLSLMPIKREFLILDQITKNGGDYFDNLMFHTSCYISMPSLGSKNSNTSTITANNNNNNNNNNNNNNNNNNNNSNPNTGIGDKINFQGNSIEEVELAINEFVNQISTYYSEKVEFTIKNNDHRVDINKLLNFNDSLAFNSNCFITCSKIDNKYTFQILGNSSNVKVATNCLSQFNSYIGETLKSQFVKFQIELPNNEKDFIAGKKNGKIIKIINMSSVSIKLLPFTDQNFVVEISGNDLMDSILGLGLFEDELPTIYNFNVPESFHRQIIGVGGQTIQIIMRKFNVFVKFSNSFELSDKTLDSQHSLQSTNFQQSFIRKNNVIIKCPSKNKFEIPLAKIELENLVNKFNILYNINRKKPTNFITELEKKTNTFIDYPSLDSISNNLNFITIKIYGIENNSKLCCLELKKIIPYSYEFKLQKDTKCLDLLHVLKLDKLLLFQSDQLSPLQLNFLNNIVVPMKLLYNIELQVKIGEQYDSIIVNYYPHSFGFKLNVTTPERLSEQSQLQVIYSNSFSQITGGMNKFLNDWSFRMIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL12</accession>
    <name>A0A1E3NL12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_33266</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017940.1">
        <property type="nucleotide sequence ID" value="XM_019162810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL12"/>
    <dbReference type="STRING" id="763406.A0A1E3NL12"/>
    <dbReference type="GeneID" id="30179497"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF46">
        <property type="entry name" value="SPS-SENSOR COMPONENT SSY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="67"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="635" mass="70893" checksum="6AC9F29965271C75" modified="2017-01-18" version="1">MVVSGQEQVDYDFHRIDQYQNIDPQEVPGSKPSSAFWSFLNSINPLRIIRIQRANRYQIQRKLNVRHLHQIALGGTLGVGLLLSSGKAFSIAGPLGCLLGFVITGFIVIATMLSFCEIVTLIPLCGGVSGIASRFVDDAFGFALGVVYWVTYMVSFPSEITASSIMLSFYPNLKVPGPNTAGWITLFYFFTLLINLFDIRVYGEFEYYSTIVKLLMLVGLMIYMIILNRGGSPPLHENIGFKYWNTELSDISKKITFGPFRPTFDIDDKGLGSLNGIGGNTGRFLQVLISSVIASYAYVGTEIVIIAGSESRNPRQAIPSATKNIFYRIVIFYLVAIFLIGLNVYSGDPRLLRYFSSDNNKAEHVAEENAIIAMIGANHCDSEIIDWGGFSNGNKSPWVIAIQSAGLCSFASAVNAFLLYFALTAASSQLYASSRTLYFMSIQNKAPSCFSLCTKRGVPYMSVLFTGSFGLLSYLAVRDNTVLVFERMVSVCSTAGLLVWSGMCLSFIRFYYALELRPDIISRNDDDYPYRSPFQPYLAYSGLILGMFLVFASGFVVFLKNEWSTAFFISSYGCLFLFVICYVGYKICRGTKTHRLDQVDLDSGRREIDRVIWEDEKNYVSSFREILIKGINLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL62</accession>
    <name>A0A1E3NL62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cysteine-rich transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72893</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYSTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017973.1">
        <property type="nucleotide sequence ID" value="XM_019164322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL62"/>
    <dbReference type="STRING" id="763406.A0A1E3NL62"/>
    <dbReference type="GeneID" id="30181009"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043240">
        <property type="entry name" value="CYSTM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028144">
        <property type="entry name" value="CYSTM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47564">
        <property type="entry name" value="CYSTEINE-RICH AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47564:SF1">
        <property type="entry name" value="CYSTEINE-RICH AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12734">
        <property type="entry name" value="CYSTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich transmembrane" evidence="5">
        <location>
            <begin position="68"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="17"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="102" mass="11809" checksum="6B3A5E88048AECDB" modified="2017-01-18" version="1">MLSRMSYQAPQGPPPGQGNQNQSYYQGEKGNMYQQQPQQGYYQQQQPPQQYYQQQPQQGYYQQQQPQPVYVQQQQPQQGRNDDCCMACLAALCVCCTLDALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NL84</accession>
    <name>A0A1E3NL84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_16711</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018015.1">
        <property type="nucleotide sequence ID" value="XM_019161315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NL84"/>
    <dbReference type="GeneID" id="30178002"/>
    <dbReference type="OrthoDB" id="10415776at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="357" mass="38475" checksum="1744FB519EC1C181" modified="2017-01-18" version="1">MTFLGELKSKTKRLHKSDKEKSKSKSSLKSIASLGSHHSHPGTSSPSKSLSSGNSLRSLYTSHSKSSKANLSSASISDSPSIAESNTASVISSPVQESLSTGETEETKISSAPLAVNDTTKTLLSSNTVDTVVSDQPDPQEKQTNTYNPTVQKTPVILDIPNFQSTTMPKQQYEDKAQKAANDVEAKAETVTKNAQKEAKNLSEKVKKDLPTSDEIDSFFEKIRKAFVSLYSSTSSTISSALGSTSRSLVALYQKSLSIIQTSAAKSIDFTKYYYSSASTKVSQNPLIAYDALYLTACSALGGAAYTYHRDFLTKKICEHCHHTNNTLHLATAVGVCLSILGVGNYLYLRKPGTKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLA7</accession>
    <name>A0A1E3NLA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72956</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018045.1">
        <property type="nucleotide sequence ID" value="XM_019164343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLA7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLA7"/>
    <dbReference type="GeneID" id="30181030"/>
    <dbReference type="OrthoDB" id="93019at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051178">
        <property type="entry name" value="TfdA_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779:SF2">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT XANTHINE DIOXYGENASE XAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779">
        <property type="entry name" value="DIOXYGENASE RV0097-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="27"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="377" mass="42122" checksum="507426C2FE5A85E9" modified="2017-01-18" version="1">MTMDILTVTPLEGQKLGAKITMPSYTNDPSKLKDEDFNTLKKALLTHSVLIVPNQEELTPESQHALNIRFDPSSAKNYGHKEELFHSSKSILAKDGKCVPRRPEVMMVGNGTFEAGHEGMKEFNLEHPSHKTFHKKLLTEEELAQGLTKFYRWHIDAALYELSPPVVTTLLGLIVPPSDERQKIIYEDTNESIDIAKGATAFASGKVAFDLLSDEEKEFALNTTVVYAPHPYIYINDCKATSDGLTIVSEEPGKEAAFESLPEWEESKVKKLPLVWTNPETGNHHLQVHGCCVYQLVDARTGKTIAELKEARETVHKMMRPAIGPDNVYCHPWTQGDLCIFHNRGVIHSVTGELDPEEKRLMHQCNIASGSDPVVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLE0</accession>
    <name>A0A1E3NLE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_22334</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454004">
        <property type="protein sequence ID" value="ODQ46163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017276.1">
        <property type="nucleotide sequence ID" value="XM_019162249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLE0"/>
    <dbReference type="STRING" id="763406.A0A1E3NLE0"/>
    <dbReference type="GeneID" id="30178936"/>
    <dbReference type="OrthoDB" id="442680at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010486">
        <property type="term" value="F:manganese:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049555">
        <property type="entry name" value="GDT1-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01214">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ46163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="250" mass="27003" checksum="766443BC57181A2D" modified="2017-01-18" version="1" fragment="single">NESSDSNSLTSPFYSFYLSISMIVVSEIGDKTFLIAAIMAMRYPRMVVFSAASSALIVMTILSGAIGHVLPQLLSPKLTRAAAAVLFLVFGVNLLREGLSVEKNQGVEEELAEVEEEIAAVELYELSSNIEKGEAGDGTTAATEQPVEWKAKLKNLAFYISSPVWFQTFSMTFLGEWGDRSQITTIAMAAGADWEMIILGGCVGHAICTLIAVLVGQYVSTKISLRTILLGGSFAFFIFSGLYGYAAVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLE7</accession>
    <name>A0A1E3NLE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_20501</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018085.1">
        <property type="nucleotide sequence ID" value="XM_019162159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLE7"/>
    <dbReference type="GeneID" id="30178846"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="26"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ46972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="356" mass="39538" checksum="42743448461CAC4C" modified="2017-01-18" version="1" fragment="single">YIHEVCITNTAKAAGDGDGDGEGIDIVLVHGYAAALGFFYTNLEGLSSIPGCRLHAIDMLGFGCSGRPSFPAPADGTTPMRKVLNAEAFFVDSFERWRLKRGIKKCHVIAHSLGGYLMSCYYLKYGRDVVEKLVLVSPVGVEDNDLSAYNRFKDRDGSEDGDEEGAGQLDDGDSAAAGDDDGDGNDRQSIDREYRELMRQIKTRVRPGKLLTRLWEWNYTPIDIVRWFGPFAGKVVAIWVYNRFARVGDAQQLWDICMYTTRMFLEPGSGEYCLGPILAPGSLGRLPLSCRLPNQLRVPVLLLYGELDWMDKTAGYALCKAINSTGGTAKFRVVPHAGHHLYVDNRAEFERQVLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLG7</accession>
    <name>A0A1E3NLG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PICMEDRAFT_16774</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018090.1">
        <property type="nucleotide sequence ID" value="XM_019161336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLG7"/>
    <dbReference type="STRING" id="763406.A0A1E3NLG7"/>
    <dbReference type="GeneID" id="30178023"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06867">
        <property type="entry name" value="PX_SNX41_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044106">
        <property type="entry name" value="PX_Snx41/Atg20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051079">
        <property type="entry name" value="Sorting_Nexin_Autophagy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979:SF2">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="9">
        <location>
            <begin position="108"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ODQ46977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="540" mass="61932" checksum="B8BD5DA7CEDD0E29" modified="2017-01-18" version="1">MDYDDFSHDNNPFAGSNHFYSNGILNPAPDLDTDEAKEDNEQHGDHNDDGNDDDDGDGDTFATSSLLDDEANTTSKSLPISASEQPTFYAPPPQLHRPAVPDSPTQQPSPYTIRTFEVGKDFKGSKTIFYKIENTINDTFTLRRYNDFKSLRSYLCKFYPYLFIPPIPEKHSISRFLKNPFNYKNDISIIELRIRLLNYFLSKLNSIPQLSNSSITIKFLDPLITNWLNCLKYPPFTNISSNSILLVSTRNPTKPSPYFSFLPIPPLALLKNFNTELNSTVFQKLELNLKIFLKISVNLESKIKKLIKTLHSLRLNMVEFGGFLNIFSIIENQNSKIEKFGNKIDLNFLNIEILINNLIIKIKEPLIILKNSIIYLLQMLHFRKLKELQLVYFQNIILKKQAKLRSLLNSINVNRSNTETQKKNFINMNSSNSPSLNLAIQNMKSTSSHSAASVNSLSDETKQLIKNEIKIMNNDLDQNLIPCFTNLLDDVNFLSTQVEKNVNLEFSSLLQLLTKILGDWNSNVWGEYLTNCLKLWESQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLJ5</accession>
    <name>A0A1E3NLJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="5 16">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PICMEDRAFT_23154</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Endoplasmic reticulum membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="5 16"/>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ47004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018117.1">
        <property type="nucleotide sequence ID" value="XM_019162299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLJ5"/>
    <dbReference type="STRING" id="763406.A0A1E3NLJ5"/>
    <dbReference type="GeneID" id="30178986"/>
    <dbReference type="OrthoDB" id="264532at2759"/>
    <dbReference type="UniPathway" id="UPA00282"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF0">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ODQ47004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="360" mass="41672" checksum="E934F5FF8739C14D" modified="2017-01-18" version="1" fragment="single">PLNLPLINRLQTVFILCHSTSILLFVFLYFSFWCIPYVWPLMAYYTLFYYLLDRSPVNGGVWYRRSDRIRRFRCWRVLVNYFPIKFHKLVDLEPTFKDTPDTENKRIDERIGPSYIFGYHPHGVIATGVSSGFTNNGTGWDEMFPGIKCFVTTLVNQFRVPFYRDYLMALGVTTVVKKNLKLLLNKGGSVVIVVGGARESLLAKPGLNSIVLNRRKGFVKLALESLDQNGLCLVPVYGFGENDIYNDSGVTFEEATKKFKKFVHQFQLFLKNNFGWTLPIVISRGIFNYDFGILPYRKSINVVFGKPIRISRLNGLTFGDPVTQDEIDYWHNEYVKGLLEVWKWGKPQFSEITDEDLKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLP1</accession>
    <name>A0A1E3NLP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_72346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017376.1">
        <property type="nucleotide sequence ID" value="XM_019164168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NLP1"/>
    <dbReference type="STRING" id="763406.A0A1E3NLP1"/>
    <dbReference type="GeneID" id="30180855"/>
    <dbReference type="OrthoDB" id="5121955at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF40">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="774"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ46263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="876" mass="100760" checksum="B30714F4069B578D" modified="2017-01-18" version="1">MSSNQMGKLKRNRQIRTCEYCYSHKLKCDRNSPCSTCVRTRSECVYQFKHLKKDQRDASAGEGRKKNLNKETSGLGGERGVKAKPKVTFKSSASSTPLYYSRAFYPFLEPSLNRMLLFKMSEVAELDFERLCSDKAEGDGFQRFGLHFSMGELADEYPGKEEFDEIIDLYWDYIHPLIPIIEKERTVANYQTFWNEFSDEENPQFDIDSGILFLSMLFAVKTAFEVNEKSPDRKEELKQQKNNILNIFEKFKLVFGLISNANLSFIQASLILYQSGSIYYIGIFTYTASLARQAEFMGLHRDPFLHDVNPNKELIKDVEIKRIVWHYIRFLDTATSITAGMSPHMIMTNSSTRFPSKRDYNSETQKFDGPLNPFMIFTICRFKCSLVMETIAHYLNSDFSSDQEKQLRWESITRTVMALYQDINALVKEIFSCSGNPKYSNVLLRWLVSNAAIIVHRTYLLHIVCDRRPYSHQNRVILKPLNGPHVGLTNLSRANSNREFFEQILTIRMPYHGAGVEVSMLLLYETSIRVGLSPELNKFKWFTKTANPFQYIYFILRDIYHYPTKVYNFTHIPKQISSFIFDDDLLNFEGDVRKFTVDISLNALYRLKDEWADPISDMMNFLLELRKFIYKSVATRNQAVPSSDFNAGGCEVEPDSTFEMDKYRSIYNLLSTLNEEQSSGSNVESIPSAKDFLDENSGHFLPRDPRNNDFMNMKYNVNVEQKDFFTNKLNNSLNSNFASNSPLDFSPDYMSPNTHNSTTYQLPSFQKSPAISPIQKLQQGPQLSPIHFSSVQSPTPPPIPSMQSPYMHPIGTLPPMLHNSGRIYTGTGRNVDFNRKEGVTYDSTVLTHTPNVSNTRLKSTISMVNRNSTSSTPPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLU2</accession>
    <name>A0A1E3NLU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_72391</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Preautophagosomal structure membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Vacuole membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="5">During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATG11 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017426.1">
        <property type="nucleotide sequence ID" value="XM_019164187.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NLU2"/>
    <dbReference type="GeneID" id="30180874"/>
    <dbReference type="OrthoDB" id="447953at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="7">
        <location>
            <begin position="1170"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="825"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="879"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1175"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ46313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1314" mass="152245" checksum="6291E3695B53C76B" modified="2017-01-18" version="1">MNNSVYNNNNRLFSASIVSTFLKGSTLLDPNRSDTNANPQSSLSIYNAFTGVKVTAPFDIFNNMDSFKVYIAQVLKIDIERLFLLTPFGIKLKFSMIVHEQINEVFAFDRKFFNPMLVRADRKDAIINELLSTIVNDELIFMIKPREPPILNANMDSFIKAIEALTYDSEEIRINSSDLDFDSLRLLLNLLKRNSGWASALLSDMKSALFNNVYYRDYEIVENILRALNSLIQYVSNLFVNLEKEFNAVLDTFNNLKVNSLSDKWESCFGLLEKISFSYTDKSTKQQRTLNLSDLINLDQTIGASKKSKILSKKINKFFGELRSLVENDIIKQKENIINEYESYKALYLKPEWEISERETMIKSKDIFLQLESHVNEMTSDSDRLPSFEELITTSSQLSTYLSQDAIKKIMSLVGQYRSQRDNYVKQITSLANELYEIQHKYSAVREELQKKVVRSMLFGVVQIQLSIRDATKLLNSELASNIELLQNSELQLSLVVDLPLLFGIWVIALLGNIKYGLSLKKISRKSNEVFEMLNYIERNNRTKWHEEFIEGIGVEKADSIFLSDNEYKNKFILENLFQFHLTSDDGGKINSTPTIKRQSSDTSNYLASFNKIIQNFNGFPNKSHQLDTIPSSTTVYNDLLKNSSISYFEGLLTSVTMKDIMDYLKCLENSEYKTSLLNQLKTYMKDMGLDEPIKLNTNKEKSVLYKSGNIDDLGSFDYEDQHYMKIFKKFIKSFESSGVTIELKIGTQKAEKPPVDTGLVRIYEERIQKLENLLHEKRFQQFNNRWSQTNSNTPIVNVVDNSAEEKIVASSSGVLGRKTIKLPPSHYTEKIQQLERENQRLNQEVEELKTSQVGEEITRMKREMEVNFLQVKNYQAKLDDNARNLEEKDKHIEGLQQRIEKLLEENKGLENKNYKLNSDITELNILNKDLLENMSHKENELMSENQLNQKEKNDLKLRIEELLDIKNQYDRVMDKVKDTDGIINNVLSILSFAFNKLRDLSKLVYGDMETFCLILEIMGLLLVDEKNGMEIKRVKGLRYTKKDLFSKKSSIDEVDDDMLFLEVVASDLINDSRSKLEWLPQMEEKLEELKGFSYQSDKVEFGESESVENTNEKLHSSKIKEIEGIFESLNNYDVKLRSVIEVYSEADLEEKYNEFIKHTLIEHDLILGRVHKRFEDVESLARKLQKEKVKLKGEIKSLTKKVSQQLVLRNFQNGDLVLFLKTLVPVTEGANTEDAKKQPWAVFNIGSPNYYLKSSILKDLSKLEEKEWFVGRIKNIEEYAITEENKDSIDKNPFNLNVGTKWYYVETKDDSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NLW1</accession>
    <name>A0A1E3NLW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_71227</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Involved in the initiation of assembly of the COPII coat required for the formation of transport vesicles from the endoplasmic reticulum (ER) and the selection of cargo molecules. Also involved in autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SEC16 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018224.1">
        <property type="nucleotide sequence ID" value="XM_019163864.1"/>
    </dbReference>
    <dbReference type="STRING" id="763406.A0A1E3NLW1"/>
    <dbReference type="GeneID" id="30180551"/>
    <dbReference type="OrthoDB" id="8918678at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024340">
        <property type="entry name" value="Sec16_CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF6">
        <property type="entry name" value="SECRETORY 16, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12932">
        <property type="entry name" value="Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="10">
        <location>
            <begin position="1052"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="9">
        <location>
            <begin position="1193"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="476"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="611"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="701"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="840"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1492"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1552"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1657"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1855"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1980"/>
            <end position="2060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2105"/>
            <end position="2194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2215"/>
            <end position="2303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="435"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="490"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="611"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="855"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="919"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1492"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1665"/>
            <end position="1679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1855"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1871"/>
            <end position="1886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1919"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1992"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2019"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="2106"/>
            <end position="2118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2119"/>
            <end position="2158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2178"/>
            <end position="2194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ47111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="2303" mass="250692" checksum="6ED1B76A7E4DC577" modified="2017-01-18" version="1">MAKGSKGKKSKAQSKNKKAKSKTSSGGNSNGVNNAENGDAIDSTNNVNTVLANESRSNPVAVENDDSESTMADDDSSLQSESSAAREAANDGDLPIDDTSLQETQTLSEGSVGLSNAHTEFVVPENDVSEIDQTSTETLEPEQVQVESTLDVALTESVPEEPPAETQKPSEPIQDKVDEAHQEPEDPFSDSNAIARCENTAKTAEQDLLQVEEPATAEDATTLEEALQDETVQGADASVLFSSEPEANIVTEETAPTGQEPLKDDVFPWDNTADQDLMPWDNKKEEESQSDIGNIELLSTQNQDIDGETVNPPATSEQVTKSLINSDAISDKADAPISFTCEQDIKIGTAPSAPAEDDKLPWDEEETADDISFPWNSAGASNGIEKESTLMISETANGIFKEDESFLNRLSPEETKTTKSNAEDKFAFLEELPEDEKLLGQNKEKTEVSDAEGPVGDKFAFFEQDDDLLLDDIMDDDLLEDDGPTNFDGANNSTANATTDTVVVSNGEKQSKYQPQQAPSNPSQNSSLLYQTNSNVFVPTHSLKQNFSHNVFTTPPPNFAEPNQKLVSNLQQQKKKTDAYDFPQDLYLKNKPKPVKEVKQNIYTQIENSIQQPNPNSAQVPSHNQEQVTNNLSFQPPPAIGGVGNRYSRSNSINSTKSSSFFAELPVSKINEKRDNLHLKNPYSLAESQSSINQVNNVERTSIGSNNSTSSPLMPPKRTNSYIPNITQSPQQENFSLQQRQPPVASNLNNKFIPSKSPPTIKKPANPYAPAAVGGHVRQISTTVTNPIDSKPNIVPVPMKSPISINSPLSPAMSVFSGNASNGAIPSNIPNAFAQKSFSSGKYAPVSSPQPPYQQHPQMIQQQYQQQQQQQQMPIQQQIQQQKQQSQPKTSMQYAPPTQMYNPPAQGSYFPSTQRGQGQPHSQVLSPQLQQQQQMIKEPQFSNGLGFDTQPQTRINSLGKNKASVKGHKHSGSSINEVYGSTIEMSNATSTGLRKSTVLPPSLSKGKHQIPPVSTLTPAPVVINPENLVRRQWPLFSFSAEDKVASMIPAFDGYSQNICNIKVVDTSSILKQDNYVSTFPGPLNKTKTKKKEVSKWLEDKIDSLNKSKDSTIFAEELTWKCLKLMLDKVEKPGDFMDRDYIKSIAALLNPSLNVTDAMNNTFDIIELTRLGKSFTPNKPFNSFSFDDSGFVTVHKMLEVGDKKSALEFAVAEGDWAMALLISNLMGPIAFDQTIKLYATTHFQGDSMGQDLSFFIQSKSQGGFSVEQLKGKESWLVENYRIVVPFVMMDNPEYGKILSGIGEALLKAGYTAYGKLSYILSGLPLIPKSLSQLPSSIYGMIVEEIYAFALLSTGNVPPNFASGFPHMIPLKIRHGGYLADIGYSVESKRYCDSTHASITSKQFFCEPATLLAQNNLCDRLSQVGSGWLSSKLSRPQLDRVWTTLDKSFNKFVSGEDIPQNEPKTEGVFAKFTSPASISRTSSTLDLTEVRNSITRKPNGNPYLSSPGHISQPLGSGSSYGGSAMVSGSRSYLPPPSMQENLMTRNVSLDSIGSYSGSSIPHPPGPNRYTPGNSLANISSESILTTASQPTSNSTLLGQPFDENRKPNIFNPAPAAKMQYSPKAPPARTLSNASLFTPAVKPMSSSPVAGRTQLAQPSFNANTFSTPSSLPPKSLKVSPVSQSYSPVLPPNLHETNTTILLEKEKHADTEIAQAHQEIEKPILPETSKSAPLPPKKKQIYLPPKEEYDSIYSVNESTKSGPALELVQPEQTLNAYEQEVGDATKDTIAVAINAEAAEKDVFPLPGGEVEDEAKKEILTKTEDSTLLSPAQKAIEADDDISQTSVDKQNTDAIATIDQTLSNDAGNTPALNESNRAHKEEVKEADELQESKQTNSNPIVSKPPPPPSKITPFRPNAGRAASRSVNRYGPSTASANKKAVNPYASIYAPKQLSPKLAYASETDSGELAADTGIPAGIPQVDMFNFGGYSTPPPAASSVNTENLDDNSNTNEVDQAEGETDAESNGKPREDIKSGLSETRSEENNFVPTYHPPTQMGVKSRKSPIQSEIKLTNMFSPPTGFAATANKASIISSPLHQFTEEKRYYAQDTGEYYDDVVDEDSDEDHGAEANRKAEEEKKKLEEDAKRRKQEEEQKRKEEEETAKKKNSSKGGESGRWFGWLGKGKSDDKPKPIKAKLGEENSFYYDEKLKRWINKKAPLEEQIAASAPPPPPAMKKVNTTPATPVGGPPTALPPTSTTNNHPSMGAPLAPRAAKKDGIDDLLTMGSAASARKGGRRGPRRGYVDVMAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM00</accession>
    <name>A0A1E3NM00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_71272</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018282.1">
        <property type="nucleotide sequence ID" value="XM_019163881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM00"/>
    <dbReference type="STRING" id="763406.A0A1E3NM00"/>
    <dbReference type="GeneID" id="30180568"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000827">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000825">
        <property type="term" value="F:inositol-1,3,4,5-tetrakisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000823">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000821">
        <property type="term" value="P:regulation of arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF103">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="281"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="374" mass="42646" checksum="DB9520DFD9F72EA4" modified="2017-01-18" version="1">MSMVPLQHKAAGHEGPMQSNDGSLFVKPTTNQEIEFYNETQNRKMQLGQEVPYGNSLCDWMPEFVGVLYPGASNDLIRETGGNIQKELLDKATDTLELEGENKQYLILNNSLYGFSQPSIMDIKLGSVLYDSSANHDKVKRMKHVSANTTSGSLKFRIAGMIIKDDFQGKLPAEIPGMKLEDVCTTEFERGYLTFNKYFGRKLTKDTVGEGLKIFFRYNKLPEKVQNLIIQNFHTRLQMLYNCLLDEEIRIVSGSLFFVFENDITRWEKLFFEDPIIAPQMTVEDDDDDDDDEEEDDEELPYYTQKDGKNMIQIRKGTTEDEYTSDSETENSDLAPLSTLKIIDFAHASYTPGKGHDEEIVSGIENLFGIIEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM74</accession>
    <name>A0A1E3NM74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018340.1">
        <property type="nucleotide sequence ID" value="XM_019160718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM74"/>
    <dbReference type="STRING" id="763406.A0A1E3NM74"/>
    <dbReference type="GeneID" id="30177405"/>
    <dbReference type="OrthoDB" id="449382at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF1">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="65743" checksum="6B5A82B9FF6CA5FE" modified="2017-01-18" version="1">MSQYKLQKNEVEVKLPSPSPDSQTYISCILSKPETDVHPETCRAAMLMHGIGGQKNYCYHSKLARKLSREQGMFVVRFDFRNCGDSSKTGVLGRTLQDDLEDMSTVYNWLTGGGFEGKKLYVDTLIGHSRGVVDVFNWQLQNQNKFVINLVACSGRFIGSGLPHSIKKLHPNFEKEGGHYIQGFQDGAYRKVWVPLKETESLGVLNMITVKNITQDTDTLSVYGSREQVIPLPDAAHYFNALAGRNTLILIPDADHCFRGVEKIPENEWETYGKPIAKPAGVVDYNPEVAEKVAEWMSSEKMHQRFYEKTKNIHKFLPRWKEVDGVANFRDIGGWNTLDGKVVRPNVAFRSAHLSTVTAKGIETLKKLGVKKVFDMRSPIESEHFKENVLSTPSGIEVVHLSEQSKGNSTLQNELFSKTLIKAALRSNAVSYVPLLETAIPTYKPIFEHLRDDIDTPIVFHCSLGKDRTGIITILLLLLCNVDPLIVAQESALSKVGVEALRPEMQHFFTAKTIDSGAEQYIKDNKPSPEWSLAKDGVDNILSIDSNAVLETITLLQNQYGGAEAYLTNKLGLSAADIAAIRKNLLFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NM80</accession>
    <name>A0A1E3NM80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TRIP4/RQT4 C2HC5-type zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_71333</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018350.1">
        <property type="nucleotide sequence ID" value="XM_019163894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NM80"/>
    <dbReference type="STRING" id="763406.A0A1E3NM80"/>
    <dbReference type="GeneID" id="30180581"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRIP4/RQT4 C2HC5-type zinc finger" evidence="3">
        <location>
            <begin position="196"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="534" mass="60840" checksum="5101B42428E6E87F" modified="2017-01-18" version="1">MSSYSMSQIKRFAADSIKDIMPLDEESINEMVQYSIRNYKTREQISNHFLDLLGPTDATFQFVTKFGDMLFGSQQPLTKSEPKPTMPTKALPTKASTPAKPTLGSNGKPAAKAKPAGIRLVKNKIVANPSSKGRLSNNSRNGATTSDMFNMKPSSIETQRVKRKEIKKKLDSIQDLDDVLLELELMDSRENLDDIRVCNCNATRHPLFEMYPNCLNCGKIICAKEGLQPCSFCGKSLMSDEEKMELKDIFNREKEELEGKNEKPKETSSTGRGKKKNVIKITLNTPGQNNFKIQEQFYKQIEENRKQEREQEQITKEEEKEIAQNRKEMEYYKSVHKKDPELIKAEERLATLLSFQDNSAERTKIIDNAADFELPTARGNLWASSMERALQLKRQQKRHKQMEDDELHRSGRGNTVVDMTVRNGKVVFNDRTRESGVFENVSDDEEIQELQQQVNKEKMKQFKEEAQNVYDYDSFNDKLFKPVYSGKPLASRGNSAGDGNATKDIISELPPLGSVVQLGEVEQQEDRLFSMIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMC3</accession>
    <name>A0A1E3NMC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_71312</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Exact function not known, phosphatase 2A performs an essential cellular function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018329.1">
        <property type="nucleotide sequence ID" value="XM_019163889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMC3"/>
    <dbReference type="STRING" id="763406.A0A1E3NMC3"/>
    <dbReference type="GeneID" id="30180576"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990813">
        <property type="term" value="P:meiotic centromeric cohesion protection in anaphase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010515">
        <property type="term" value="P:negative regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="11">
        <location>
            <begin position="155"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="350" mass="39947" checksum="4BA6D420797F8067" modified="2017-01-18" version="1">MDLDSDVQMEDAIDQQHTQQSPQSPQLINSASNLNDIPVSTVDPKTQINTLEGWMEQLSKCQPLSEDSVKELCNMAIEVVKNEQNVQPVHVPVTICGDIHGQFHDLMELFKIGGPCPDTNYLFMGDYVDRGYYSVETVSYLVAMKIRYPKRITILRGNHESRQITQVYGFYDECFRKYGSSNVWKIFTDLFDYFPITALVENSIFCLHGGLSPMIETIDQVRELNRFQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGFTFGQDISEQFNHTNNLSLTARAHQLVMEGYSWSHQDLVVTIFSAPNYCYRCGNQAAIMEVDENHNRDFLQYDPSVRPGEPTVSRKTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NMK1</accession>
    <name>A0A1E3NMK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome B pre-mRNA-processing protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_72872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454003">
        <property type="protein sequence ID" value="ODQ46838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019017951.1">
        <property type="nucleotide sequence ID" value="XM_019164315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NMK1"/>
    <dbReference type="GeneID" id="30181002"/>
    <dbReference type="OrthoDB" id="2107880at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061671">
        <property type="term" value="C:Cbp3p-Cbp6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037653">
        <property type="entry name" value="Cbp6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28250">
        <property type="entry name" value="CYTOCHROME B PRE-MRNA-PROCESSING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28250:SF1">
        <property type="entry name" value="CYTOCHROME B PRE-MRNA-PROCESSING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20180">
        <property type="entry name" value="UQCC2_CBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ46838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18126" checksum="75A912057FB9F09A" modified="2017-01-18" version="1">MSANGTTKKIVALLQQLPADKVKHYASFKYTQIERFCNIGGIPVPKAVKEEQAAEDKKKQVLIKIDAAKLKRMIFSEKEEQPDYQKNLFNEDILKQQYKSLKNIHEKKWGDFYAISSKLHEPKGNPNYYTRLLGDVNKGGQKKEGLFAAFKTIISGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NN39</accession>
    <name>A0A1E3NN39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alternative oxidase</fullName>
            <ecNumber evidence="17">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PICMEDRAFT_30997</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="16 17">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the alternative oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="17"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018634.1">
        <property type="nucleotide sequence ID" value="XM_019162666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NN39"/>
    <dbReference type="STRING" id="763406.A0A1E3NN39"/>
    <dbReference type="GeneID" id="30179353"/>
    <dbReference type="OrthoDB" id="16906at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000002">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005229">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="14 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005229-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ODQ47521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="293" mass="34139" checksum="3A4D33B8328419E4" modified="2017-01-18" version="1">MTTKEEKKEGPFLVKSVYEHPGYSKEDMHAVKYEHRPVQSFRDAITFGFMVGLRRSFDLCTGYIEPRDEKHQIEIGKGPNRMTVEKWLTRVIILESVAGIPGAVAGFLRHLNSIRMFRRDMAFIDTLYDEAFNERMHLLTFLKMAKPTIFTRALLWVGQGLFANAFFMVYLVSPKTCHRFVGYLEEEAVSTYTRCIRDMELGLCPELEQQPVPQIAKDYWNMGDDATMYDLIQYIRADEAKHREVNHTFANLQQKGEDRNPFALTIEDDPRPQPSRTLKNHHAKGWEKNELIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNA7</accession>
    <name>A0A1E3NNA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_15123</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018241.1">
        <property type="nucleotide sequence ID" value="XM_019160678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNA7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNA7"/>
    <dbReference type="GeneID" id="30177365"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042307">
        <property type="term" value="P:positive regulation of protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035617">
        <property type="term" value="P:stress granule disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000770">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="307"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="187"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="516"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="547"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="816" mass="92374" checksum="02C1FC0D8B47503D" modified="2017-01-18" version="1">MPSSNSGSNYTSNDNIHDEKIRHAVEASPNILTHDTEFDKLRRQEQLPHHSQPASSKLSLALQKQQKSNPGSLNTSVINRDESLINAEVERKLTIPDASSFTDSSSSNSKSSTLSHQNPNHQTLARSITSTDAKGKKNHGLSVLTSINEPFAEVSEEMVHSDVVGSNVVTITETKILKQTLKNNLLHDADSPDVNRRRNTSGSEHFNTHRELINDINGDTHRNDFYDMDTDKKKDMDDEEEEDTDDEDLNEQDLHVVDFSLIEDDEDDDEIDPNNRGVKEAEEDLSEYKEGGYHPAYIGETYKNGRYILVRKLGWGHFSTVWLAKDTLNNVHVAMKVVRSAQGYRETAVDEIKLLTKINITDEEHPGHQYLIKLIDYFDHKGCNGTHICIVFEVLGENLLGLIRRYKHKGLPIKFVKQIAKEILLAIDFLHRKCGIIHTDIKPENILIGIYEVEALLNYLEETQWERHILKKISSRILSNGNGSIDTKSLTNLAKARNASYGKTRYGKRRANTIVTDSQPLPSPLRSHSNSFFNSPLSRSLNNNNSANTDENQQNIPIPLSASSNLNSGSIARSSSFTSSELPSRMSYKDIQSIMDKQNETEKSLFDYVPPSADEIVDDDVIRVKIADLGNACWTFKHFTNDIQTRQYRSPEVILGADWGCSADIWSIGCLIFELLTGDFLFEPTVGQSFSKNDDHLAQIMELLGPIPEELVKSSHYGRRYFHSNLRTLRKIQNLKPWPLESVLLEKYKFSEQDSLEVADFLKGMLVLDPKRRMDAAGLSNHYWLNDANVEGHIDREVGTRGEDIGEGWYREISKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNB1</accession>
    <name>A0A1E3NNB1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71249</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018251.1">
        <property type="nucleotide sequence ID" value="XM_019163871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNB1"/>
    <dbReference type="STRING" id="763406.A0A1E3NNB1"/>
    <dbReference type="GeneID" id="30180558"/>
    <dbReference type="OrthoDB" id="515064at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22212">
        <property type="entry name" value="NDFIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF5">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1014"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1047"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1125"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="828"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="954"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="144"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="209"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="840"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1174" mass="131279" checksum="4321EB2C0130BEA4" modified="2017-01-18" version="1">MGGNENLGGMPLGLNQGESQIPMLQQHQQPQQQQQQPQQQQQQQQQQQQQQQQQQQQQQPQPQVSGQGQGQGRPGLSAQQILTYLMRLDPEQRNAVISKNPQLRAFLVQIEQQRRLQIQRMQQQQQPNAAPNTTGIASTDSDLNNNTRPHPNTNPNAQQLPMQNQLSHMQQQQQKQQQLQQQQLQQQQQHQLQQQQQQQHRQQKMPQQFSPQNISGSIASMKTGSVSASHSPAELHTSTANPSFATSTEGNTAFSFGPTLQPTDLSNLPKDKIPRKPLQKKGMKKKALTGGPIPIGVGSDTIASPPLVRANPAIVSTQVPGNNLNPIPQPLQMHTTQQPVPTVPLNANGPTAHPGAVEPKPSNETVLATEDPKGRFADFTNPLLQHPHEWSERLKKEDMEPSTDLKIYENIITKDLDYLKYASAIVNAAKENENKVNDKVGLNGKLFNRILKDLKFYQQVKAARMQSMKLPPEANGLTSNMWGDGYSGYGNGFTNGKIEFVFPENSKMKYTDENLNEDSENLYQDGESIPIRLEFDVDRDGFKLNDTFLWNSNDDDETLKVFVNELVKDYRIENNVSNVKQKILASIKEQIADLHPMVVNPNIDLRFPINLDITISNNQLVDRFDWDLMNQENSPEEFAETLCDEFALPNEFRTAIAHCIREQCQIYLKSLYMIGYKFDGGVIVSEDLKEFLRSGFDQNNVMMPRYLLSAFTPSVQELSIDNFERIIKERERESKRKKKGVTRVGRRGGFVLPDLSNMPKTFRTPLPTAIFPGGVAVVDNEHSAADADYTDIPIDIEVPADQLRRVHEKDVEEKRALELLVERVRRSSVKPDQHVSAHTAGAGDAADSADSAAVDSAMDVDMAGKDDIEADPDQDVKMENSEDVPLIMMPIQERIEDMAARGRESLRSIYNNAPLLRYINPNNRYNQVPSSEENDPARTRTVQGHVVHVGETHDGVFSNMPAKPSTSPASVPDQLPSYDEASHDPSPPYWETSIMSEFDEIYIDGIPVGNVFNFIWSAAVSVLFQFLGFVITYLLHTSHAAKNGAQFGLGITIINLGLSSLPIDITKHEIDSTTGRFEPADPSTIDVSLQNGADLPGQLDGYHSALSTHGDFRESPGSLLNGTPILAYFLFTLGGFIVAKATYDFYKVKRLEYSIMNPMSSAQVRPEETPEESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNB4</accession>
    <name>A0A1E3NNB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_71646</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AIM24 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018707.1">
        <property type="nucleotide sequence ID" value="XM_019163973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNB4"/>
    <dbReference type="GeneID" id="30180660"/>
    <dbReference type="OrthoDB" id="5295771at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ47594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="323" mass="36080" checksum="E7A89FE711F1DB2C" modified="2017-01-18" version="1">MIHQQSRSISFQSIKSLFKSTRPTPATELQQPLTSLPNATGNGAFVEPISFQVLGASSNLLNIKLPRSSILNIRYSNYQQKIIAINGHINSMYTELARSNENNLIFQRCFNQSEPMSLLMSLNAQNANFAVLNTLKFNWIIKRSSLFAWSGPSIKPASSDADANLIQMNGEGTFVVATPGQIIQINLSENESLQVNSKSLVGYSTTNDKLSDSIAELNNGVKSVVDVSVKKAPLFLRLNWMRRYFSVPEKFWNEPTVHQITQTLSSMSRALKTFFNYVRAKLISSKRSGYFIDVKGPRTVFLSNAVNVNDQILTDSELKKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNS5</accession>
    <name>A0A1E3NNS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription activator of gluconeogenesis ERT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PICMEDRAFT_25503</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transcription factor which regulates nonfermentable carbon utilization. Activator of gluconeogenetic genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERT1/acuK family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018857.1">
        <property type="nucleotide sequence ID" value="XM_019162396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNS5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNS5"/>
    <dbReference type="GeneID" id="30179083"/>
    <dbReference type="OrthoDB" id="2538135at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045991">
        <property type="term" value="P:carbon catabolite activation of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045013">
        <property type="term" value="P:carbon catabolite repression of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR OF GLUCONEOGENESIS ERT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="13">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ODQ47744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="453" mass="50543" checksum="E92F2A7A2E8542CE" modified="2017-01-18" version="1" fragment="single">RRVPAKKVSRACVHCRNAHITCDDSRPCQRCVKKGLSDSCVDAPRKVKKYLLGGNGLEKTAAGPGDAVYDSIDGTLGSPSRNDQAEINFLSSAADSEYAILGNIIDQTMFQNDGNSHNPASTATSQKYVSPALSTTSEDFEYLHYQPLMAQSSGNSPMSMGQSRSSMRMDDAPKSPFYSDLYPDKPQCDTSTNQYFIGTMSTIDGVKTHTFPEVVKQIAKFKNEHPKRFRERNRRSAISFSIGILDDVATVDGNGGRCGLLYHEPCEIYAKVKQPFTYVKPYHDLNLYLKSRFPKSDLVQMSKSIAEYRPSFIAGMIKLKEDDLIFAEQCFQRTLLEYDGYIGISGTPTLVWRRSSQIAYVGDEFCILTGWSKENLLGKSTFAVEIMDDRSCVEYFKIFSKIAFGDMSGDTITECTLLTPKGESIRTNSTWTLKRDVFGIPMMIIATFLPILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNU5</accession>
    <name>A0A1E3NNU5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_20925</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018867.1">
        <property type="nucleotide sequence ID" value="XM_019162175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNU5"/>
    <dbReference type="STRING" id="763406.A0A1E3NNU5"/>
    <dbReference type="GeneID" id="30178862"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="42"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="298"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ47754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="603" mass="68041" checksum="236D3194CED65614" modified="2017-01-18" version="1" fragment="single">DSDSEDELDDKKLKSEEALKDYCPGGYHPTFIGEKYGRSEEYLIVRKLGWGHFSTVWLAWDSARSRHVAIKIVRSSSNYSEAAMDEITLLEKVNSGNGAHEGKKHVVQLLDHFIHEGPNGKHVCMIFEVLGENMLNFESYGGLPLTLVKQISKQILLALDFLHRECGIIHTDIKPENILVEIHDVEKLVQLLEFERKSKKLNKMLEKRHNDHSYCLQHTNSYPVGQLQSSMTHSTTTSNTNIKPNNITNNNTNYNSISNTINSTTGLGNKNTITSSQRFSNHSYHSLSRSISIQKSLSNNNANSNATTGGININTPMRKNSIPIRSSKPLTSPVETSSVDNFFRSFSFSQRRNPSFSSGSVQNNIGNLTNNSSTINANNVSPVLNQNSSFERHGSIVSSTSQPSILNEFEEIISVKIADLGNACWYNKHYTPDIQTRQYRSPEVILGGDWGCSADIWSAACVIFELITSDYLFDPKSSASYSRNDDHLAQIVELLQTWPSKDYLRTCSRWREFFDRSGQNFRKIGKLKIWPLRMVLIDEYKMSEELATEVSDFLLPMLEFTPMKRVDAGSMCSHPWIKDVSCGADLGREYGLHGDGIKGWKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNV2</accession>
    <name>A0A1E3NNV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_32286</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018887.1">
        <property type="nucleotide sequence ID" value="XM_019162754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNV2"/>
    <dbReference type="STRING" id="763406.A0A1E3NNV2"/>
    <dbReference type="GeneID" id="30179441"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="42"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="42"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="97"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="97"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17322" checksum="A59233180C197D56" modified="2017-01-18" version="1" fragment="single">MNNSLSSGPSLKSKKTLKSPNKAHIPQHQDYSQHPHQTQQQSSKLRRGPWSPEEDKHLLDLVSLFGGEKNLNWVKISQMLETRSAKQARERYHQNLKPSLNRTPITPEEGLLIEELVDRYGKRWAEIARHLNGRSDNAIKNWWNGGASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NNX7</accession>
    <name>A0A1E3NNX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_27004</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018908.1">
        <property type="nucleotide sequence ID" value="XM_019162472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NNX7"/>
    <dbReference type="STRING" id="763406.A0A1E3NNX7"/>
    <dbReference type="GeneID" id="30179159"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ47795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="238" mass="26807" checksum="3515DD6DE06F736D" modified="2017-01-18" version="1" fragment="single">ELRQHILCSLLNEVEIMRGLNHRNIVHYNHTLPSPRHLYIIMEYSAHGSLLKYIRSQPRQCVSELISAGITAQVLEGLVYLANLSIVHGDVKAANVLLFPHGVIKLCDFGLSFQWDDENTEDYDDYHLVSKENSNKQLQKIAANGSAYWLAPEIILHRMATPKSDIWSLGATVLEMLTGFPPFSNRGPLSACHAVGSGTKIDYPHGISDGCKLFLESCFQYNPTLRSRARTLQNHPWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP56</accession>
    <name>A0A1E3NP56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_15741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018975.1">
        <property type="nucleotide sequence ID" value="XM_019160943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP56"/>
    <dbReference type="GeneID" id="30177630"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ47862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="52" mass="6019" checksum="41D604141978F0F4" modified="2017-01-18" version="1" fragment="single">MHDALSDYESMDESEQEPEDANVENQKAEYENSSSEEEDLKYTEMLSNAQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NP84</accession>
    <name>A0A1E3NP84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_41523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019002.1">
        <property type="nucleotide sequence ID" value="XM_019163115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NP84"/>
    <dbReference type="STRING" id="763406.A0A1E3NP84"/>
    <dbReference type="GeneID" id="30179802"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="309" mass="35581" checksum="BC18F944BADE226C" modified="2017-01-18" version="1">MTLALKKLDPNDYITLENTYLDRIALREKLFDETQFYACDDSAVGALKEMYRFIFDFLVKKYPQYFVLTEKETLIHNTITGKFFSSDPADMKKEEILRLIATNIEEDMLIMLKNPSTGQLDEYILRSFITLMPAGFNPMEKLNKSLTAIHGPVPGYLQKLQISMNKFFSRVQPHEFIVRQNWSLQTHSNLCAPKGSHATKEEAEKLHPLFPNDLDFNKCFFRVEKQCVTRLPESGAIMMIVRTYTTSLMDLRADLSEEQKDIMCSAIDGISGDLALYKRRVIWGDAAKSFIRGESNGSQPIPRKHVFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPJ7</accession>
    <name>A0A1E3NPJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_15816</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019059.1">
        <property type="nucleotide sequence ID" value="XM_019160977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPJ7"/>
    <dbReference type="GeneID" id="30177664"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ47946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="94" mass="10591" checksum="774B3AF034548898" modified="2017-01-18" version="1">MIELNKRLFATANRILNGNSRWLVITWSTFCGRSHIFIVCFFVVRGQAKGVLTQYVYSFTNEKCLFLAGEAFGSAVSSVVPVGKRNGILWEVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPQ2</accession>
    <name>A0A1E3NPQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_57972</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRP1/MDG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018636.1">
        <property type="nucleotide sequence ID" value="XM_019163269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPQ2"/>
    <dbReference type="STRING" id="763406.A0A1E3NPQ2"/>
    <dbReference type="GeneID" id="30179956"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF81">
        <property type="entry name" value="CRUCIFORM DNA-RECOGNIZING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="202" mass="22515" checksum="31983C03A28D5B30" modified="2017-01-18" version="1">MSTGYHVFKWPTVHANEVYVSGSFDGWTSRHRLEKDREQDCFRVQCRIPREKKVYYKFIVDGEWYTSEEDTTESDEHGNVNNVVVYHAGDGDGDGSSGDTDADAAVIVHDSDGSSEFTSISHPNSETRWEEIQQEVEAANAAGDEDAEGDEDEDLTSSVQITLKDTSSVTASIQSRTTPSTKLTVPKHGDSFMGRLRSIFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPS3</accession>
    <name>A0A1E3NPS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_31510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019139.1">
        <property type="nucleotide sequence ID" value="XM_019162707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPS3"/>
    <dbReference type="STRING" id="763406.A0A1E3NPS3"/>
    <dbReference type="GeneID" id="30179394"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015665">
        <property type="term" value="F:alcohol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:monoatomic cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015850">
        <property type="term" value="P:organic hydroxy compound transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF34">
        <property type="entry name" value="PROTEIN HOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="61396" checksum="ADB8D1D69B61EEAC" modified="2017-01-18" version="1">MEKYTDRYHPQFIPGTYSIYSSAVIRHQHHMAIKRYRYGVILQPQPTNSRNDPLNWSKRRKTLHSLILLLVTAFTAALSNDASAPTDSINAITGISYDLLNDSAGVLFVSIAVSTWLYSPFDYLLGRKSVILFGAIFAFFSSIWYALMNDGGDSYGSQVLTGFSFGCADAHVQLCFASIFFRHQLGAIITIYNLAYSLGTYLGPLCANFISFDRTFRWVGWSGAIASGVLILIVLFLFEENSFDYAKYSHRVDDLTLNLGFVQNGVISNEESDDALTQGYFDKPFSYLERYKPFRLPKDPQCRTLLGFVKHYFQLLLIPVRCIVFPPVIYAGCICGLQNAILTFYLTTEDTMLYSDPFNYSSAQVALMNIPCIIGSVIGCLYAGSMTDYFILWMARKNSGIVESEFRLYFAFLSGTVGAVGLLMFGFGISRNMDWRVFYIGLAFISYMFSSSTNLAMLYVMDTYRELTLEVLVCVAFINNIIGCIFTFACSPWLQSSGTENTYIALAVISLGVMHSSGGFIYFGKRWRKRTHAAYVRLAEIKADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPU9</accession>
    <name>A0A1E3NPU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inactive metallocarboxypeptidase ECM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Inactive metallocarboxypeptidase ecm14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PICMEDRAFT_15926</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Inactive carboxypeptidase that may play a role in cell wall organization and biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019198.1">
        <property type="nucleotide sequence ID" value="XM_019161024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPU9"/>
    <dbReference type="STRING" id="763406.A0A1E3NPU9"/>
    <dbReference type="GeneID" id="30177711"/>
    <dbReference type="OrthoDB" id="3626597at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000060">
        <property type="entry name" value="Putative metallocarboxypeptidase ecm14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF147">
        <property type="entry name" value="INACTIVE METALLOCARBOXYPEPTIDASE ECM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133475" description="Inactive metallocarboxypeptidase ECM14" evidence="13">
        <location>
            <begin position="24"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="14">
        <location>
            <begin position="216"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ODQ48085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="528" mass="60975" checksum="1F68E2E76E0A8CE6" modified="2017-01-18" version="1">MKLYTSQVLLLLACVCNLHVCNALLFFQNWFQPVENAVVDTKTFLLHDRVLRFANEVEVFEESRHDLSQTIAQLNSANIKHYSQDPHNVPVPLEQYQGQYILRLYDWNEKLENRLALKQQPSFFEFAFTIVRQLEVPIWEKSTTHKFIDVQVTKSQGEELLSLLSMVYSGPDDFQSKIMILDLPQSVYETFSVSELDSGDAVVDDISTNAEIFFKSYRDLDTIYSWFDLLVDTYPDILQVEYIGNTFEGRPMKALRLSVHSDSKVDSIKTVVITSGIHAREWVSISSSCFILFQLLSDYENGNLAVRRYLENLDFLFLPVMNPDGYVNTWKNERLWRKNKQQTYNPRCAGIDIDHSFGFHFTTGYESPCSEDYAGESEFEALESYNWDNYLNETKHSHPIYAYIDLHSYAEEVLYPYAYSCDILPRDEENLLELAYGLGQAIRLKSGKYYEVLKACEDKGADLLPSMGAGSALDYMYHNKAYWAFVLKLRDSGTHGFLLPSKYIVPVGKEIYAAIKYFAAFLLSDKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NPW4</accession>
    <name>A0A1E3NPW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_123971</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019267.1">
        <property type="nucleotide sequence ID" value="XM_019159921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NPW4"/>
    <dbReference type="STRING" id="763406.A0A1E3NPW4"/>
    <dbReference type="GeneID" id="30176608"/>
    <dbReference type="OrthoDB" id="201635at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.20.20:FF:000011">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="205" mass="23103" checksum="EF0AD72014051927" modified="2017-01-18" version="1">MFVQKPFATALRASARCAARQTRAGYAVTSYRGFASTRCFLNSEKAAGKETAAEEAKELVEMKDLYVRSIADFRNLQETTKREIQKAKDFALQKFAKDLIESIDNFGHALSAVKEDSVKANDEIKQLYDGVKLTRDVFEKTLTRHGMKKIDPMDEEFDPNKHEATFQAPNQEKEPGTVFFVQQPGYELNGRVLRAAKVGIVKAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ02</accession>
    <name>A0A1E3NQ02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PICMEDRAFT_15949</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019229.1">
        <property type="nucleotide sequence ID" value="XM_019161032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ02"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ02"/>
    <dbReference type="GeneID" id="30177719"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000117">
        <property type="entry name" value="Glycosyltransferase family 34 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF5">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ODQ48116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="366" mass="43581" checksum="F779ED20B244214D" modified="2017-01-18" version="1">MQRTGMLDAGSKETVDPMDLTSDYDNYTYDTSVSSLDYSKMLKGRNAFRLKGGFQKVKSFIQEHKFVRYVLFLIPLFVFAYLITYPSHKDNSPSKIVMILAVNEGGGVERWKTPQEWSVERSSIANKKAYAQRHGYTLTLKDTSLKRRYSHEWREGWEKVDILRQTMREYPDAEWFWWLDTYTFIMEPELSLESYLLEDINKKAYRSVEHFNPLGIKTDVPYVNTLDQPIDMILAQDCGGFNLGSFLIRRSEWTDLLLDAWWDPVLYEQMHMVWEHKEQDCLEVLYSQQPWIRERLGFVPLKAINSFPDGACEDQKDDKRYFYSKKDRDFIVNMAGCNFGDRSCWDEYENFRNLKAELHRPWYKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ34</accession>
    <name>A0A1E3NQ34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Initiation-specific alpha-1,6-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_31984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ47828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019018941.1">
        <property type="nucleotide sequence ID" value="XM_019162734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ34"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ34"/>
    <dbReference type="GeneID" id="30179421"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009133451" description="Initiation-specific alpha-1,6-mannosyltransferase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ47828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="45118" checksum="8C4A814FC0903C1A" modified="2017-01-18" version="1">MALGYLLFLVNGLSSTATSKAISEFYRFNPHTRGTNPYDYKTTADRLSKSFPYDDSPRRIDKNIFQMWKVPITDPDFPHRNLVSKWRNENPTYKYNLLTDAEIHDKLVMHFSNTVPEVVEAFDLMPNKIIQSDFARYLLIFLFGGLYSDIDTRLEKPVDTWFDSDRNVGFVVAIEEDMNVENWEHYMSRRIQFEQWTFKAKPRHPILRKLIAKIVETTFNAAKNNKLQAYYKDFKGVDKCSSVDIMEWTGPVVWTDTIYGHLNSLENPTIVDIDSTRSITGELYGPPLDKEQLISWKFFTGLVAPVMVDDVVIYPKASFREDTENNCGKYCYIFHEFGGSWKNNGKGEIKPKDGLLEDEDVAPQQKKKLVKRGLFSDDSDCDHPYSPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ42</accession>
    <name>A0A1E3NQ42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_32168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019347.1">
        <property type="nucleotide sequence ID" value="XM_019162746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ42"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ42"/>
    <dbReference type="GeneID" id="30179433"/>
    <dbReference type="OrthoDB" id="16516at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06143">
        <property type="entry name" value="PAN2_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050785">
        <property type="entry name" value="PAN2-PAN3_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048841">
        <property type="entry name" value="PAN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728">
        <property type="entry name" value="DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728:SF0">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20770">
        <property type="entry name" value="PAN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="474"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1081" mass="122086" checksum="D84E700480887876" modified="2017-01-18" version="1">MIQLNTTSVVRHPKNQAITSSDAQVLVSCVKFDDSHSLLWVGDSRGYLTSYSGPKMQLYTSVRAHNGPILKILNHKRGVLSLSFNSIRLGTKEGITLFNILSDELKGINSMSYTSNTQTELLVAGDKDATQSRIFKIDMINKRISSSFAYPHTVIFMETNLKYIVLGRSDGCVDILDPKSNRILESFECHSSGLSHISVKDSSLLTTGFSSKRGQFVPDNFVNSFDLKTLTASSPISFPAGASKVFHHPIIPNVILISSSMGHMNFLDIKTPSKLNIYQADITTYVTAFDLSSSGSFIAIVDALHNLHLWSRNAMGSSTEFALYNAPLTFPTPIAEVIPASNHLTSPNSPFSLIKLPAFHSQLLSAWPSDLVFDVGGLPNQIDPEILRSSELINGILIARYNKEKFGPRNVAEKYYNISQQTSEGANDLYNHEIFDLKSARGDVPNAYRQLSILYSKFGVDDFDFDFYNKTKFSGLEINSGNSFFNPILQLYRYIAPMFNYTLMALAEDVTEESNLLMELGYLYDMMHKSDGRHCAASNFQIIFSSIGETQNLGLTTDSNISRDDYKQRRMIQTFNRFLLERLSKDECKLYKTQTPNNLNDICGVYTETTIYSNFCSLTHKRVMMYHSIDINCLPSPPLIPTSVTILNYLEASMNKHIQQQIVCENCGYQHPVNASLVINNLSPVVVLNLDLNNQQMNEIRYLKGWLTPEFYYAQSPLGTPVLRSNVIGGVVSNLRKFELCGYTAQITNRQGESHLVTYSKIRTDPNDSGRWYLFNDFLVREVPEEEVLDISPWWKKPVVMIYKEVGIGNEFKPNVYMNNLNTSILHKDCLIEGTREGKTIEYHLLDNNETIKPGTLVALDAEFIELLPAEYEFSGEGTKSLVRPPTLSLARISVIRGEGPKEGECFIDDYIATSKHVHDYKTEFSGIVAGDLTPGVSKKSLVNLQVAYRRIWLLLNLGCVFVGHWLAGDFRMINIYVPPEQVRDTGLCFYLKKEKRKLGLKFLAHYVLDKEVQKGNHDSIEDSINALLLYKEYLKLKESGRLENMLFHLYFEGQMSRFKVPTEVTLRKPSNTKKDDNVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ52</accession>
    <name>A0A1E3NQ52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PICMEDRAFT_16006</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019308.1">
        <property type="nucleotide sequence ID" value="XM_019161052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ52"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ52"/>
    <dbReference type="GeneID" id="30177739"/>
    <dbReference type="OrthoDB" id="148331at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072697">
        <property type="term" value="P:protein localization to cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000009">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ODQ48195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="331" mass="38363" checksum="7C3347E66F5684A9" modified="2017-01-18" version="1">MLQLEPQNLLIQEAFAAALSPGYEPVSLDRILTDFDFTTYHVSTPQEKTRILLSIGIKCWQDLEKYGAMTLLEKKYGGYKFIQILSQGACEPGYDVSLYIDTEQCELDEEGKAELINELSLLKRNCFAAPFLKAFARYEVLAKENPVDPNNLYGEDSVATSNSNEEVLKLDYRGEESIYIKPSSDRVTVIFSTVFSDETDKIFSKVFLQEFSDARKRSIQKAPQVINSHKEVPLEIQHVAPPAGDKTYITFVLFPRHLSTDDVKWNTITHIQLFRSYFHYHIKIVKCYLHQRMRFRVQSFTKILNRARREVDEEEMRAERKTASGRRFEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQ56</accession>
    <name>A0A1E3NQ56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Polyadenylation factor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PICMEDRAFT_16047</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for 3'-end cleavage and polyadenylation of pre-mRNAs. Also involved in chromosome segregation where it has a role in chromosome attachment to the mitotic spindle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019350.1">
        <property type="nucleotide sequence ID" value="XM_019161071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQ56"/>
    <dbReference type="STRING" id="763406.A0A1E3NQ56"/>
    <dbReference type="GeneID" id="30177758"/>
    <dbReference type="OrthoDB" id="16717at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="170"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="212"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="392"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ODQ48237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="483" mass="53340" checksum="C4D2CDD0937EE971" modified="2017-01-18" version="1">MSGLSRRNHDAESELEKKLQQRRTVDYFTPVGKYMITKNVYNRRRRRALPIGSIRPAASYVTDLPPSLISSPSIVDLSSKFVHISTNKSKHAIHAVKWTPDARRVLVSSYSGEFTLWNGLTFNFESIMQAHDVPIFSLEYSHSGKWLLSGDMSGCIKFWQPNFNNVNILSKAHENTVGELAFSPNDSKFLSCSDDQTLKVWDFSTATEECTLKGHHWDVKGCDWHSSLGLVVSGSKDNLVKLWDPRDGTCITTLHDFKHTVSKTVFQKGGNERLLAACSRDHSTRIFDLRMLRAVSVIKSDKETDLTSLAWHPIHSTMLSIGGYDGSLSHYDVSKTADIEAFSRTAPKSELNSDKTSGSVSKSGGGNVPDYLLNTYVNRGAPIVSDAWHSIPYAHDRAIYTMEYHPLGHMLCTAGADKSMRFWCRARPGDEAGFNDAAHLGAAAANVAKVDDAREVSGTESRADLGDATEGSVLPGFSIPGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQC0</accession>
    <name>A0A1E3NQC0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_11167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454002">
        <property type="protein sequence ID" value="ODQ48265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019378.1">
        <property type="nucleotide sequence ID" value="XM_019159765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQC0"/>
    <dbReference type="STRING" id="763406.A0A1E3NQC0"/>
    <dbReference type="GeneID" id="30176452"/>
    <dbReference type="OrthoDB" id="4064682at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2790">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ48265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="311" mass="35388" checksum="FB10DD193A311BCE" modified="2017-01-18" version="1">MSFNEKPVSTIDLGLPSEAQVLAQFFDQDFNPSDYINALFMTSLNASAPISNALSNKSLELNSASSLKILFKRCSALSLHFNEYTNELVRRFDQSYEKLHSSSSQIISYDGQNQLGLSLKSSDDDTTAESKEIVTRLQYHLATLNTSMYSLLDDLKQTREKLDIIDPKKTNKNAVDDLQKLVLVKKRVEEVKISFNLLKSLVASSEIEDAREDFQNAATFKSDRITVAEFKNALAVLRKLMQEQLSQEIDLYNNSKKDGHPAEVNEKLMKIIDNMINLQPIFKSFLNFQSSYAAFVEFLKEQKSNYLNLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NQY1</accession>
    <name>A0A1E3NQY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_70100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019579.1">
        <property type="nucleotide sequence ID" value="XM_019163550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NQY1"/>
    <dbReference type="STRING" id="763406.A0A1E3NQY1"/>
    <dbReference type="GeneID" id="30180237"/>
    <dbReference type="OrthoDB" id="3994232at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="680" mass="76933" checksum="2B038E837FCB29FA" modified="2017-01-18" version="1">MEIKKKKKSTDYSRRGCLQCKKSHVKCDEKFPTCSRCLKRSTECKYFTNFLLESTASTIERNTTAKTTIVWENNQFTNVKDRAAANGGLVFPGDADYSARAAAVHDEANVSPSSSTSNLSTTDKILNPTHVFLPAPMTPANMNIHYHLGEDPAKLTALQHSKLIPSPSFLNNFNSKSPHNASVSDSSDVGSQFSFSHIKLNDLISVGQSDLDYLSSLNKEYKTMTYLYNEKTHPDLHVFDIPWDGGPMYYFVDTIKKYDPIVTNSEISLNDQSIIDFTWTLARITKFFYTFVLYSETSLMSVLDLCFKLGTKSSIFQSILTYHCSVHVVRLYNISNNENLADLWDVNVRIPTFKQCIDYLRAGLENSPTFSDLVILTFAVVIIFSGNASDESWRAHLNGCYQLISKSYTLKSSANLEDSFDVAALALYDVIVEWYNHTASLAAVSAGNGFLGRDLSSLRNITTSNIAVASNGMNLMAGHCSNITDLISNINKFMHTSQKKGLKLSGLNFVYFILNENKSNDAAAEITVNGFEFLRQLNKIKLNFEYERLELEDYKMDLSIKYCNLLYMDGLKLFITYFFIGTRDKSIIRSILRDILDLIYSMPYRSSCAIICHWNIYIGGLVSLLISDFEIYGHFVGILKVFQLNGMDVQSIDILEKIKSILFEKDYQQLLYADNDFVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRF2</accession>
    <name>A0A1E3NRF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70271</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019764.1">
        <property type="nucleotide sequence ID" value="XM_019163598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRF2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRF2"/>
    <dbReference type="GeneID" id="30180285"/>
    <dbReference type="OrthoDB" id="24683at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000299">
        <property type="entry name" value="Zinc finger protein 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051879">
        <property type="entry name" value="C2H2-ZF_Maturation_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="144" mass="16896" checksum="E92763521960C77F" modified="2017-01-18" version="1">MGRYSVKRYKTKRRTRDLDLILNDLSSPDSIQKLKQQPEDENLPGLGQYYCIHCAKYFLDNTALKGHLRGKVHKRRVKELSVNPYTSLESEAATGTNLEKFMEKVAKYKANEGPRREMEHGMLKDQTEEYDLRDRKKWEELVGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRF4</accession>
    <name>A0A1E3NRF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PICMEDRAFT_26393</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019762.1">
        <property type="nucleotide sequence ID" value="XM_019162448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRF4"/>
    <dbReference type="STRING" id="763406.A0A1E3NRF4"/>
    <dbReference type="GeneID" id="30179135"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="217"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ODQ48649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="547" mass="63234" checksum="5DBE01AEF24C1C5B" modified="2017-01-18" version="1" fragment="single">VAEPHSSSSKLTSSGSQYSNNASLNKDPRALRHTETVNYGATHLKHQREQVIKLAGGRITVYEYGRSHEHDYSKYKTHPNKPMMGIFNWMKKKESSKTEREDSYEIIEKSVSLLPDKYSGDLMRVKNNPKNYIWNYDDDEDEEDEYVEDDDDEEDDDDDDFDDEVDNMMIEPIIGKEQVFLINSMLNKIEHPEKFKSRLEEKEREGKIKLMLSQKYGHIEGVVGKGSYGTVCISSKTVGTTKIYFAIKQLKRKQGESLHHFGNRVTSEFMISSSLTHQAVINVYDLMVDPVSMTYSEIMEFIPCGDLFSLISTTNGLHIVECDCFFKQILNAITYLHSVGVSHNDLKVENLLLTRKGQLKIIDFGTSAVFRTAWESDVQLSRGACGSERYVAPEQYNKEREYDPRLGDVWSLGIIYLTMYYGKYAWEAAKMSDETYARFIDTRAVFDYSYKSSTKAHQFLCVKPGGYSPIEGIKGEYVNDSRKYVLYNILNPDPTFRMRTYQIWQSEWIKNFHVCDAGRGYVSYDNYIEMALRSAQDVEAREREYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRN0</accession>
    <name>A0A1E3NRN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_28731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019849.1">
        <property type="nucleotide sequence ID" value="XM_019162553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRN0"/>
    <dbReference type="STRING" id="763406.A0A1E3NRN0"/>
    <dbReference type="GeneID" id="30179240"/>
    <dbReference type="OrthoDB" id="2018507at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF65">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE AKL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="34"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="310" mass="35342" checksum="0B134A0DE3B81593" modified="2017-01-18" version="1" fragment="single">MRDVAAHQNAPVGRPPSLVKLPPGTQLTVGKHQVTIVKYLSEGGFAHIYVVKTNPMERGTDVACLKRVIVPNKEGLNQLRAEVEVMQRLAKADNIVRYYDSNASRLPNAPGCYEVLVLMELCPNKSLLDFMNSRLRTKLSASEILKIMLDISKAVYNMHRMKLIHRDIKIENVLLDENFNFKLADFGSTCPILRVPRNHQEFQILHNDILLQTTPQYRCPEMIDLYRGLPVDEKSDIWALGVFLYKLCYYTTPFEVAGELSILHAAYSFPAQPHFPSGIRNLINIMLQENPVFRPNIYQVLMEVCKLSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NRY2</accession>
    <name>A0A1E3NRY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Signal recognition particle subunit SRP68</fullName>
            <shortName evidence="10">SRP68</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70428</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SRP68 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019942.1">
        <property type="nucleotide sequence ID" value="XM_019163640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NRY2"/>
    <dbReference type="STRING" id="763406.A0A1E3NRY2"/>
    <dbReference type="GeneID" id="30180327"/>
    <dbReference type="OrthoDB" id="10255118at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15481">
        <property type="entry name" value="SRP68-RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034652">
        <property type="entry name" value="SRP68-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038995">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0733">Signal recognition particle</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="586" mass="67152" checksum="4B068795A37698D4" modified="2017-01-18" version="1">MDFPLANTYGARSELFLQSADDYHRQRHRINKRLNKLRRNLNIVTKDTKNYKEKCKENSISAENYDMDTAFGDLWLYRIERDLLYAQETRLLLDVHTSKSKERFLVSKYKKALKNAHHLLSVIANEQDQYKVLEILTYTAIIDGSLAVTRRKYGEALYTFSVARCSLQFLYAHQNLPATFTKELYYDIVDLVVDPALKVAALQTKSSTISDLSQLSKEQVFSNRGKVSYLGKAVQIVEKEAPSYITPSNEEESEVLREITWSSYTAKISSNEIALAIMKVNEQLKQVVDSDTSSYDSALMSYLDAISFQEQEMERSEADADDAESQEQHIVLTYLKYNYLLLRIRRDIAIMKDLDNKATAKNNLSRQKVLELWKDYLKVNDSILDSFNEIKELPGIANDDDIADMIIKLDVFFQIKKQMKLAQAYLIFNGYVKSLALVSHCDELIEGVKPFTDGDDLKGNLPKESDIEDLKMKVKEEKSKLFILASYFKDNGDSAPVGSKYLIDDVKKFPDYSATQLLENIAPLKVDFEPVNVKPVLFDIGYNYIKYEGAGERNILKKSTDEPMRDSVSDDSIDRKKGGFFGLFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS32</accession>
    <name>A0A1E3NS32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_9057</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019631.1">
        <property type="nucleotide sequence ID" value="XM_019165057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS32"/>
    <dbReference type="STRING" id="763406.A0A1E3NS32"/>
    <dbReference type="GeneID" id="30181744"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="11">
        <location>
            <begin position="23"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="437" mass="48759" checksum="E83F9AB7739A4597" modified="2017-01-18" version="1">MAKLIQRLSVLPLPNYKNTLKNSTLQLNANANDTLRCLLILLTRRLQRMSSLSFIMMMNPHMARIWDAYLHSYGELIQFIAQYNLPENGTLDLDFDHLLDFRVTREEENQKFVSVLQDIMDMHTDNVIDLRDGIHETKLMGKAGSDASDDDSLFAGFSEKQFLDEHLAERVLMRLIANNHILLSQQKKGNLPGDADVSSGVLDNNLNVLDVLSRSVEFVNDMSSLKYDERVDVRVDTRLIDENGDYTCEENVDLRNIGDHKKLVFPYIGNHIEYVLNEILKNSTRALIENRVQKPVDILIVLDRSKPDSAPTLQIKISDHGGGIGPDTLSKLWEYSFTTVSNGKSSSKGARNNDNQNALLSTQSGKDIELGAAHDLGFGKSDGEESGADSVVSDNLIAGMGYGLPLSLTYCQLFGGDIRLHSVWGKGTDVYVVLKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS38</accession>
    <name>A0A1E3NS38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PICMEDRAFT_14445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020038.1">
        <property type="nucleotide sequence ID" value="XM_019160377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS38"/>
    <dbReference type="GeneID" id="30177064"/>
    <dbReference type="OrthoDB" id="3993235at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CNH" evidence="2">
        <location>
            <begin position="772"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ODQ48925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1087" mass="123818" checksum="D62D4384CDA06EEB" modified="2017-01-18" version="1">MCCDGNGKAMQPSQKHKQSKYFESLKLRSSQSELAPIIPPLNVSPTTGTSPLLSPTLSSSTAATAATAATAATAATVETESSIERKPTITPLQSPFQGQSNKSSSTLSPLHSSMAVDRIDSSPLRLHLQTDSNGPVSSSSSSTLQNRFVSSSPASKSHTPSPPKRFSKHRLNNTENINYTLNANLSPPVISRLVSSSPTKTIVGGASPTSPNINISHSDNSDFVNNNDHHLYDDSHQLDVEFFDDLSLLENNETQISFFKEPMLSPVQTDNKALFESPTTTIFRKRSHQLFSPMKDNKTSFVNMSQLPLDMKIICNSRAENNFHTMNGFVNVSYMEFWNLTSEEGKTYKDWDKTQFEIQSLVFEIFTNLKKIRFNLHRLIFIYGLEFKDTGFISDNIYDKTFEVLQEFYYYLDKLVVKKLKPLFDDHFFVDDAQVLKILAGWFKQLKLQYRHISGSVVYLSKLSSNEDVRKLIIGISKKDLENSSNRSAVSPIELFNSYFVKLFTSIELLFDRLKSVYKGANYIKNFDVTDDLQQVIKEINNISDSTSELEKKISFNEKLSYKTEIDFSHMEMVDMFNVKRKSKEPLHLEMKSNLNWTQAILAPFDNYLVILQVKSNAINLNKKDEYVLCKYPISIQYLEVDTYVDGNYKMVIIKDIGNKAIYHFRRANDITVAILDRFLKDLHILKADFWAQGKLNKKIELKIFNANSFISKSESYDPFNLAPIPDTFNLVNLEVSKGGKDNELNLDEFEPILTNVLSCDYFSYKHEGVSEKLCLVGTTFGIFIGKLNDSASFHKVHQITNVKKITVMNNEVVFCIGGESLYRLSIEKLFHTYKFRLPASDINVFQENKRYISDFTVGYQSSAKDNGCPYLFAWNSKLVYYSKILKDTDWKFIWQSFKTHYNITRLQTVYANNFAVGHIVDGSAVWNLSKLSDIRSIGLNQLDVKDILKSETPVAIFPFPNQEKNISEVLVVFSKFATRMKNVKGKYIQSSDEIIWFGMQCDNASFDCEEKILITVGKQAVEIRALYDDITRKSDLIGCLFGSSFSLINEMPGKAILKVQDIKGHDEYKKQIIFKIRRLKNPGKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS44</accession>
    <name>A0A1E3NS44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PICMEDRAFT_14470</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020070.1">
        <property type="nucleotide sequence ID" value="XM_019160389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS44"/>
    <dbReference type="STRING" id="763406.A0A1E3NS44"/>
    <dbReference type="GeneID" id="30177076"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16489">
        <property type="entry name" value="mRING-CH-C4HC2H_ZNRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000510">
        <property type="entry name" value="Phosphatidylinositol 3,5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="22">
        <location>
            <begin position="165"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="478"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="249"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="310"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ODQ48957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="525" mass="57594" checksum="86BD8AB7AF3289B1" modified="2017-01-18" version="1">MLVRRDSDTPYQDVRVSPRASSVTLPTALSASHTSTISSSPPATTMATATVTNGVNTSRVRPTEISSENPAMNGEPPKEPSRISLSLSKLLPLIGKDGIGSSTNNSAASGNARNIDQTIGEGAHQPHGEEAVENDHLDVAEDTPTGTNIHNGVATRDEEAIWQEDNGVKACPLCDAKFTFFNRRHHCRKCGKIFCNKCCGKFCTFIPGSSVVEPEEEGGRSIVRNGYQYYKFRTCEKCYGEIKMLKEALGIADDSEEDSREEDTEEEEEEEEEEDESAEGGGDDNESFERHLSIMGEDRSGNACGPEWRRSRRRAGARTRSRTGRETSIEGPVRSQGEEGDDADAESLTCLIKHSQPREITMEVSESRVREAHGTTGGAGAGEGPRRGNGNTSIDHNNNSNNNNNSELNECPVCGIDIGGVSETERETHINRCLTDQEFGSPTHPGDVAKRDKNRMLVYSIPADADMAHMVLNDDNECVICLEEFCPGDKLSRLECLCCFHYKCIKDWIRKKGYCECPIHALHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NS72</accession>
    <name>A0A1E3NS72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PICMEDRAFT_29560</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy; may act as an adapter for ATG8 that supports mitophagosome assembly. Negatively regulates the proteolytic processing of ATG32 via the i-AAA protease. Acts as a negative regulator of the m-AAA protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. The mitochondrial prohibitin complex interacts with the m-AAA protease, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. The mitochondrial prohibitin complex interacts with ATG8 and the interaction may support mitophagosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the prohibitin family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020025.1">
        <property type="nucleotide sequence ID" value="XM_019162598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NS72"/>
    <dbReference type="STRING" id="763406.A0A1E3NS72"/>
    <dbReference type="GeneID" id="30179285"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035632">
        <property type="term" value="C:mitochondrial prohibitin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140580">
        <property type="term" value="F:mitochondrion autophagosome adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="10">
        <location>
            <begin position="63"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ODQ48912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="313" mass="34400" checksum="C562E7A8C0E55F6E" modified="2017-01-18" version="1">MNSNDWQRIAKDLQKRAEDISKSAGGPGRQGFRPGSGGSPKGALFGAGGLALAAAAFFVVQGSLYNVNGGERAVIYDRLTGVKAEVVGEGTHFKIPFLQFPTIYDIRAKPRTVASLTGTKDLQMVNISCRVLSKPDMHALPTIHRTLGKDYDERVLPSIVNEVLKAVVAQFNAIQLITQREKVSRLVRENLVRRAAYFNISLDDVSLTAMTFSPEFSTAVEAKQISQQDAQRATFVVDKAIQEKQYMVVKAQGEAKSAELIGDAIRKSKDYVELKRLETAKEIAEILSKSPNRVFLDNEALLLNTVTDSRSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSB7</accession>
    <name>A0A1E3NSB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Autophagy-related protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_70106</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ATG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019586.1">
        <property type="nucleotide sequence ID" value="XM_019163552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSB7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSB7"/>
    <dbReference type="GeneID" id="30180239"/>
    <dbReference type="OrthoDB" id="338614at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097632">
        <property type="term" value="C:extrinsic component of phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01486">
        <property type="entry name" value="Apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000243">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.100">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.70">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006285">
        <property type="entry name" value="Atg7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032197">
        <property type="entry name" value="Atg7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042522">
        <property type="entry name" value="Atg7_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042523">
        <property type="entry name" value="Atg7_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01381">
        <property type="entry name" value="E1_like_apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16420">
        <property type="entry name" value="ATG7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like modifier-activating enzyme Atg7 N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="10">
        <location>
            <begin position="310"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606285-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ48473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="650" mass="73239" checksum="65FCBDD4B60F8A9D" modified="2017-01-18" version="1">MTDQLMYAPAVSYADSSFFVELSALKLDVLKLDTSFQDIVAYYNYSALGTEQPPSLNVSDSSFKGDSYLAKQLPPQSFFLSRGKLFNVNSIEEFKNVNKKDLLTESALSIWNKIKDGSFTKDPSILSSFILLSYADLKKYKFYYWFAFPQLQSEWVQIKTSPNVQFNVRDRLFEQQFYVISSDSDDSLLPLTSLFDQDKSFLKILFIDTCSYENTTAYILRNFFSALAYYNFHKVEVQVVKHSQPSLSFTTCLENANYNSTELTDPQYLPPVSGWEKTSRGKLGPKLANLGSLIDPLQLADQAIDLNLKLMKWRLVPQLNLQLVKDSRVLILGSGTLGSYTARCLLAWGVRKITFVDNGKVSFSNPVRQPLFNYADCLDGGSPKAETAAANLKRIFPLVNATGFTLEVPMIGHSVKDEVKERADFEQLEKLVDEHDAIFLLLDSREARWLPTVLGNSKNKIVINAALGFESYLVMRHGCVDPTKSLSEQSSGRLGCYFCNDIVAPTDSTSDRTLDQMCTVTRPGVALMASSLATELFVSVLQTPERQYSKHVAVDRSNILGSLPHQLRGFLHNFELLKVSSNNFKCCTACSIPVIQAFNDEGWEFVKRSLNESTYLEDLTGLKKFHEEAERAALIMEDLDDLDLEDEEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSE7</accession>
    <name>A0A1E3NSE7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PICMEDRAFT_14497</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020105.1">
        <property type="nucleotide sequence ID" value="XM_019160402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSE7"/>
    <dbReference type="STRING" id="763406.A0A1E3NSE7"/>
    <dbReference type="GeneID" id="30177089"/>
    <dbReference type="OrthoDB" id="4055624at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018843">
        <property type="entry name" value="Utp8_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053881">
        <property type="entry name" value="Utp8_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10395">
        <property type="entry name" value="Utp8_b_propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22542">
        <property type="entry name" value="Utp8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Utp8 beta-propeller" evidence="2">
        <location>
            <begin position="6"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Utp8 C-terminal" evidence="3">
        <location>
            <begin position="384"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ODQ48992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="720" mass="80923" checksum="239E89041396DBBC" modified="2017-01-18" version="1" fragment="single">NNHLLNDKKLFAIGYTERKKSKLKFITYGIEENNNGSNLEDENQSSSMSVVNEEERHTNELENIKEVRDRSISVDSEIIGLKFSKDSKSVFSISANGVINVWKFSVDTNEIETPILNFNADGLAHKKRRVIFHKFFKPEELKIDSRSEKVEQLLLTAETCSKRDSIIVRIYSINSNEVLEISSSTISGLGSIDELQFTHEISGKLIILKHEPELVIKSYDLPLIDNEQTLKIGGVFKHEPQDAPTSIMCASTNRVLVTKGSTVALIDIQYEALLSSLDLYSRSKEQNVNAKPPRNATLLSVPTVAGNTLRSKKTFALLVLKNTKENYSQIQYVSIDVGLGKLRDALVSLPDSEENGAENFISFTPYISSNEISDVDAEVKYLNEQVLKNNEEMRDVYKKLKEIKGSGDMSLLESRMISYLKNAPYGESHSDNDFKVYEYEKDRFVDPKFFKLCTLLLFNYNAQTDSISLDSENIPETALTYVLTHPLFPTPYATGLLRVLEVSPRLQRQAIVTCVNIPCHDFILELSSAENDEIFKDIVNRLIDEFSSEEITQETVKIMKKKPGLGSKSNGTGFDLDKIIGKIIKLNYGYEVLNSFIDSNGLVLSLHYANDDTQLAKLIKQTQTKVKSMIDDTALLTLVNQTLTNAELGREQEKSKKASKKSLRNSKPSSVSAGASQLGEIEVETNKLDMMLKVGGRDSQFSKKTTRTKVSPYTIDRLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSI3</accession>
    <name>A0A1E3NSI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PICMEDRAFT_14581</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020208.1">
        <property type="nucleotide sequence ID" value="XM_019160447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSI3"/>
    <dbReference type="STRING" id="763406.A0A1E3NSI3"/>
    <dbReference type="GeneID" id="30177134"/>
    <dbReference type="OrthoDB" id="8191639at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF45">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="11">
        <location>
            <begin position="104"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ODQ49095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="275" mass="30932" checksum="AC8AF45A7C6E67B0" modified="2017-01-18" version="1">MSSNWLKLLQKQKQKQKQQEQKQPAAKDDVSSHRHSKSAAAGCSSRKSQGRKAPKSVVTMRTPLTKIARDQAEAKVEAAHKAQALRKALVLSQPLSDRQQEVGKYLAMDCEFVGVGPGGVQSELARVSIVNFHGHVIYDEYVRPKEKVTDWRTFVSGIRPSDMHKAITFDQAQRDVAQLLKDKVLVGHAIHHDLEALYLTHPKHSIRDTAKHTPFKTKYSKGKTPGLKKLAQEILGLDIQSGEHSSVEDARATMLIYKSDRKSFEEKVRPTYHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NSS0</accession>
    <name>A0A1E3NSS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PICMEDRAFT_14250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ48718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019019831.1">
        <property type="nucleotide sequence ID" value="XM_019160292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NSS0"/>
    <dbReference type="STRING" id="763406.A0A1E3NSS0"/>
    <dbReference type="GeneID" id="30176979"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="54"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ODQ48718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="319" mass="35715" checksum="78E5961FFC169919" modified="2017-01-18" version="1">MGSLFVLIRSFPSSLPWFRCFSTSHQLFKTASSNRWLNRQNNDYFTRRAKVENYKSRAAFKLLQIDERFKIFRSKSSSPRNTLNVLDLGAAPGAWNQVALDRSPHGSKILGVDILPYQPPDGASTLQGNILSKGTHQQIKNFFTLSELEKKDEIFNDVEIITSSKSTPEPMESDASILEKEIEVSQLMGKELSNKDNGPAADPASSGSDLTASYPVDVVLSDMMANTTGLALKDHVMSMDLCDAALITAVDLLKKNGSLVMKFFSGEDDVVLEKRLNAVFREVKRFKPKACRLESKECYFVCLGKRAYDVDKVELFSMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NST1</accession>
    <name>A0A1E3NST1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PICMEDRAFT_91682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020217.1">
        <property type="nucleotide sequence ID" value="XM_019165073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NST1"/>
    <dbReference type="GeneID" id="30181760"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ODQ49104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="222" mass="23390" checksum="B411DB2F645F076C" modified="2017-01-18" version="1">MSFLPIAATANATTASCTTVPQPYLAGRKHTCRPCTDQAPDAKNFAAPRYADTLLRPARRRRSRPTPLALLPQPNITNCLAASATAAAAAAPSPVARANHFMCTLHPPGAAASAATCSRHGREWRNNSGAAFAGCVVPILAGCVTACCQNCFSNGGIGPCVHSSFARERKLAVPGGKTSIVHMHAQHIKSHPQAHGLEADVCKWKWIICPFCLGLCPQLGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NT19</accession>
    <name>A0A1E3NT19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">5-aminolevulinate synthase</fullName>
            <ecNumber evidence="18">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PICMEDRAFT_14757</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>succinyl-CoA + glycine + H(+) = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6 18">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5 18">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 17">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="18"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020398.1">
        <property type="nucleotide sequence ID" value="XM_019160516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NT19"/>
    <dbReference type="STRING" id="763406.A0A1E3NT19"/>
    <dbReference type="GeneID" id="30177203"/>
    <dbReference type="OrthoDB" id="10263824at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902117">
        <property type="term" value="P:positive regulation of organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015118">
        <property type="entry name" value="5aminolev_synth_preseq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09029">
        <property type="entry name" value="Preseq_ALAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 17" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="5-aminolevulinate synthase presequence" evidence="20">
        <location>
            <begin position="8"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="19">
        <location>
            <begin position="139"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU910713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF09029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ODQ49285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="567" mass="62441" checksum="79A35A3B92BDED2A" modified="2017-01-18" version="1">MESLTKTSLRVCPFVKSHSSSAMKQLAKRSGLLAEAAHCPVMGPAIEAKRNYSHPSKAACAQATDSITATKINHDATAAAKAAAELHSFDSNEKATDYNGFFDNQLNKKREDNSYRFFNNINRLAKEFPKAHLQDEADKVMVWCSNDYLGMGKNKEVFDQMIKTMEKYGVGAGGTRNIAGHNRHAINLESEVAALHKKEGALVFSSCFVANDAVLSLFGKQFKDLVIFSDELNHASMITGIRNSRAKKHIFKHNDLEHLEAQLAMYPKSTPKIIAFESVYSMCGSVAPIEKICDLAEKYGALTFLDEVHAVGMYGPHGAGVAEHMDFKAHLASGIASPNYKTVMDRVDMITGTLGKSFGVVGGYVAANRSMTDWIRSFAPGFIFTTSLPPSVMAGLSTSIRIVRKNVEDRINQQLNVKYVKDEFKRAGIPVIPNPSHIVPLLVGNAADAKKASDCLLDKYKIYVQAINFPTVSTGEERLRITPSPHHDKQICDQLIESVDDLWRDLDLKRVKDWENIGGHCGVGSATHTPLENLWTDEFLQLTNADLNPNVLNPVVEQLEVSSGIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTG3</accession>
    <name>A0A1E3NTG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PHD finger-like domain-containing protein 5A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_29988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020419.1">
        <property type="nucleotide sequence ID" value="XM_019162616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTG3"/>
    <dbReference type="STRING" id="763406.A0A1E3NTG3"/>
    <dbReference type="GeneID" id="30179303"/>
    <dbReference type="OrthoDB" id="10248186at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005345">
        <property type="entry name" value="PHF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13120">
        <property type="entry name" value="PHD FINGER-LIKE DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03660">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016468">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12524" checksum="DE622704849A1853" modified="2017-01-18" version="1">MSRHQLDLVVCSRLPGTRVGVLCSKCDGRCPTCDSYVNPQHVVRVCDDCSFGSLADTCIICGNAAKPGEAMHDAYYCTQCCLLERDRDGCPRVLNVGVSRSDNFYQQKKKGISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTN6</accession>
    <name>A0A1E3NTN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_71070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL Y-2026</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020638.1">
        <property type="nucleotide sequence ID" value="XM_019163819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTN6"/>
    <dbReference type="GeneID" id="30180506"/>
    <dbReference type="OrthoDB" id="3997689at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ODQ49525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="503" mass="58496" checksum="AEFC965312126AC5" modified="2017-01-18" version="1">MTEKLSELEKRPSPEVVNVADSSDEEVYETDVPFPNRLRQRRAKVYQNYDENEEDEEDYNEDDEDYDDDDMGETSMPNGEDETTRETFSPEIVGALPERRIITDDVYTAQHGGERPVVNEEYIEYGDSSMDEEEMIDGRKVIAHIADDFFATYKPNKIQPTFPLLDLPAETKKFCNILNDANIDFATDLPLHLYNIYLLTTRNRELPPAKFTLWPLPAHELITPRSFLRTTDFESLGSHANDTARMLEETKYTYVNYDDNSKLIKPSAHMLEFWHPKINPLAELDEALDAIYERKINSQIKKYSQDHKVTKKGFPSRCTYQRRTPEDIHLSPLLKEKIITKLDSIIDKLTDYHTNSIVKTQKISRRLGTNGKTPKRASAVTYGLDWFSVLSCLDDKDKHSKMLLLKLFNLKLDKDTINGPVNSYPIENELYTATKIRRDKILKNLICKSKVHLKNAKPTQYAKFARKQKNNISYNLDSFLNLGLYYTKHKRLKERNYSTESDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTQ4</accession>
    <name>A0A1E3NTQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor SYF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_10003</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020650.1">
        <property type="nucleotide sequence ID" value="XM_019159615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTQ4"/>
    <dbReference type="STRING" id="763406.A0A1E3NTQ4"/>
    <dbReference type="GeneID" id="30176302"/>
    <dbReference type="OrthoDB" id="10067343at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056350">
        <property type="entry name" value="HAT_Syf1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23220">
        <property type="entry name" value="HAT_Syf1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="15"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor SYF1 central HAT repeats" evidence="10">
        <location>
            <begin position="268"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="11">
        <location>
            <begin position="425"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="830"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="870" mass="101497" checksum="88D59F317946F156" modified="2017-01-18" version="1">MQNEDPLLECIEEADIGFEMQLLKDLSPNEDLWNQYINHKREQVIENAKDVKTKQSLLALLYRRSRVWPESYEHWTQFIDYYIKTVQINAPRDMAEVNKIVQCFELASKTFQKKTVFWLKYLGFLINSVKVVDIGFILEMFSVSLLKLKPNEHLPLWTLLFSELLDKVKLPQNMKFDIFFSFFIYLKNSDKFGISTGEEHENETPSYDQIFSVLLSTLSSWQQVTKFQKFFNEMIKPETLLKLSDSVLELYRRYLEKLIKLSTENEFPEKVKLETKISSMFQEIVKKFPDQQSVFTIKFAKYLMMKGEFDAAMQTLEARLNSSLTIKDFSVIYDSLTEILEEKVSELSEKSQDETVLSVFLNKLEFLLSNRLLLLNDVKLRQNINSPSTWLERIHLFEEEGEESSDKLFKCYSKAVLSIDPKKIPKDEKIQFPQLWCDYAKVYLAAGEIQTARTLFETATKVPWTEMEQLEYVWIEWIKSEIGLKNLEHAEFVCSKAVSIPEQISSGKIDIDDENISVQMRLFKSTRLWSLYLDLAENCEDFDDICKVYEDAMELKVINGVMIINYCLFLEENGYIEKSFSIFERGMGMFNGESKSILYCIYLNKILKYWDKLQWDVERVREIFEEGLGFFSEEQNGKSLKQVYILYAEWEMKYGSKIRSLKILKEGISQMKRQGDKLVMYKILIVNTIECKGVEWAVEVFQEATENLSVRIPGYISDIICGFVEVEVALGNIGRGREVLQYAAENVMEFCKSHEDRISIWELFKAFELENGDENTYKEMLRRKRYLENVFGPTETAPTSVTDSAKPTEESANARIRKEIQDRIGFVASSEGPKKTQIIIEQSPHTSGDSSDTGEGGRRNNDAIDLDMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTT5</accession>
    <name>A0A1E3NTT5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lanosterol 14-alpha demethylase</fullName>
            <ecNumber evidence="11">1.14.14.154</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome P450 51</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cytochrome P450-14DM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Cytochrome P450-LIA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sterol 14-alpha demethylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="ORF">PICMEDRAFT_71099</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28 29">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75107"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:75108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75111"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:75112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta(14) steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:68068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:68069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68064"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:68065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54028"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:68061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>lanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25286"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:25287"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75103"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:75104"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 26">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.154" evidence="11"/>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020668.1">
        <property type="nucleotide sequence ID" value="XM_019163825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTT5"/>
    <dbReference type="STRING" id="763406.A0A1E3NTT5"/>
    <dbReference type="GeneID" id="30180512"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008398">
        <property type="term" value="F:sterol 14-demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11042">
        <property type="entry name" value="CYP51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000033">
        <property type="entry name" value="14-alpha sterol demethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 25 26" id="KW-0349">Heme</keyword>
    <keyword evidence="7 25 26" id="KW-0408">Iron</keyword>
    <keyword evidence="9 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 25 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 26" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 26" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="27" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="25">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="ODQ49555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="527" mass="59944" checksum="231936DEB9044F79" modified="2017-01-18" version="1">MSVIKTLVQDAHHLLSIAYGVFSELSTIQQIFSIIAIPFIYSLTWQFLYSLRKDRVPMVFYYLPWVGSAVTYGMQPYEFFESCREKHGDCFSFLLLGKVMTVYLGPKGHEFVLNAKLSDVSAEEAYTHLTTPVFGKGVIYDCPNWKLMEQKKFAKVALTKESFVRYVPLIKEEMMSYFNAAFKGSSGTADVLKAQSEMTLFTASRSLFGDELRSKLDTSYAEMYSDLDKGFTPLNFVFSYLPLPNYFKRDAAHNKIAHTYLDLITQRRDSGKIENRDLVDALLKNSVYRDGSRMTDREIANLMIGVLMGGQHTSSATSSWFLLHLGEKPELQEQLYQEIKSVLGDRSELTYDDLQKLDLVNSTIKETLRLHMPLHSIFRKVTRNLPVPNTSYVVPKGHYVMISPGYTMINERYFPNAGEFQPHRWDDIKSIDGGISLPATSNGGSDEQVDYGFGKISKGVASPYLPFGGGRHRCIGEPFAYTQLGTLLVTYILNFKWTSKCPPVDYTSMVTLPIQPALINWERREKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NTW8</accession>
    <name>A0A1E3NTW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PICMEDRAFT_14934</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="9882" last="9887">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goeker M."/>
                <person name="Salamov A.A."/>
                <person name="Wisecaver J.H."/>
                <person name="Long T.M."/>
                <person name="Calvey C.H."/>
                <person name="Aerts A.L."/>
                <person name="Barry K.W."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K.M."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E.A."/>
                <person name="Lipzen A.M."/>
                <person name="Meier-Kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J.L."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
            <dbReference type="PubMed" id="27535936"/>
            <dbReference type="DOI" id="10.1073/pnas.1603941113"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL Y-2026</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NRAP family.</text>
    </comment>
    <dbReference type="EMBL" id="KV454001">
        <property type="protein sequence ID" value="ODQ49489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_019020602.1">
        <property type="nucleotide sequence ID" value="XM_019160601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E3NTW8"/>
    <dbReference type="STRING" id="763406.A0A1E3NTW8"/>
    <dbReference type="GeneID" id="30177288"/>
    <dbReference type="OrthoDB" id="10251401at2759"/>
    <dbReference type="Proteomes" id="UP000094455">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="214"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="369"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="522"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="713"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="912"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="1096"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ODQ49489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000094455"/>
        </source>
    </evidence>
    <sequence length="1239" mass="139411" checksum="C0E77683FFA09FB7" modified="2017-01-18" version="1">MVKRQRRRDDVDGANGAVGAVGAEGASTGTDVSGAASNGSGAARNETDGGRSTDTAKHEKDEDDDEDEEEDGDEDEAAEQEQKPQRTKLAKRQKLSAHDLQVARETAELFKSNIFKLEIDELIKELKLKDSHCLLIEKILHKLHSIIQLIPQSDPLAFDDAVAYFDSSNVSIPFPDPKPNFLNYPLQYTAPDDVSLIGSFGLKTGIQQKNGMSVDVCLTMQKSLIQQKDYLNYRVLYKQAFYLAYLTHHLSILAPQHNLPIKLTYDYHNGNKLCPSLVISSDPEASSSKKDLLFNKTNFSIRILIGLPFGTFESKKLLPDRNCIRIQNSSVTSIEDLPPTPLYNASILSSTTYSYYLKYLYTTKKSTESFKDAAALGKLWLSQRGFSSNVNEGGFGHFEFNMLMAALLQGGGTSSNKILLHGYSCYQLFKGTIRYIATQDICDDGHLSFSSLIGDNKSVYKKSGFNIPTVFDKNTKINLLWKMSKSSYSILKKYAIETMDLLNDVVKDRFNQIFLTNNTNSYLKYDSVVSMPYSKLIELNEDKFGSLEKISFVTLENYLCNKIYKILLEALDDRVYQIDVHLSQNTPSWSTGKRKPSNNLNNSLHLIIGLFFNPSESDKRVTKGPLHTEKELGEKFVSFWGDKAQVRRYKDTTVQWSCLWDVSPTDSVVLTIVKYVLDRHMSNSANDSHLLSNDIIVNSSKFTSLLPSPLVPHSSNSQPLILPQHFILLKNSFDELAKDLLALDLTLKVRSLLPTSPSLRNTSPLLPVPFATGNPDFYNDCVLQFESSSRWPDEINALEDTKTAFLLKVCSELLEKNSDEYIDAYIVKDKSSVPYNSNISVLRILKSDGYGFAIRVITENDEFLYLRAIESASKATGPSQLASVELSYIRFMQKYINAVKHHRSISILATHFPYYSATVRLFKLWLDSQMILNHIPEEVVELIVLQSFVDPAPYGVPGSVMAGFLRVLSFLSKWNWREEPLILDLSREFNGNYEVNPDKESYVDRISDRLDLTTFQTINQSFTKLRESDPQGVKVPYFIGTKEDISGKVWTRGISLVIASRLTSLSRVVLDVLSKNGQNGVEISDQFIKLIFTPALKDYDVVIKLDSAKLNSKKLRRLSGVLPTNIKFKNLVEPLTRYEEAEKSSDPAINYYKDLISRFGEVVVWSVRKYGGLVDANSDRVITGLVVPGNQSRKFRVGIAYPIQPTDGGKDLVRLDVEDVLKNCAVLGGDMIHTIEQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E3NUE1</accession>
    <name>A0A1E3NUE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PICMEDRAFT_9735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pichia membranifaciens NRRL Y-2026</name>
        <dbReference type="NCBI Taxonomy" id="763406"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Pichiales</taxon>
            <taxon>Pichiaceae</taxon>
            <taxon>Pichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3"
[file cut: first 33554432 bytes kept]
[document cut: first 30696799 of 33554432 characters kept]
